U.S. patent number 3,858,796 [Application Number 05/306,218] was granted by the patent office on 1975-01-07 for container for use in treatment of liquid.
Invention is credited to Stephan L. Schwartz, Hans Peter Olof Unger, Eric J. H. Westberg.
United States Patent |
3,858,796 |
Unger , et al. |
January 7, 1975 |
CONTAINER FOR USE IN TREATMENT OF LIQUID
Abstract
A container for use in batch treatment of a liquid, particularly
blood, such as centrifugal separation of blood into fractions of
different densities and/or for washing blood cells suspended in a
liquid, comprises walls of flexible sheet material which define
between them a closed compartment for holding a quantity of liquid
and a conduit which communicates with the compartment. The conduit
is adapted to be acted on by a peristaltic pump for moving liquid
through the conduit to or from the compartment or alternatively the
conduit prevent liquid flow through the conduit.
Inventors: |
Unger; Hans Peter Olof
(Lidingo, SW), Westberg; Eric J. H. (Stockholm,
SW), Schwartz; Stephan L. (Lidingo, SW) |
Family
ID: |
26654433 |
Appl.
No.: |
05/306,218 |
Filed: |
November 14, 1972 |
Related U.S. Patent Documents
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Application
Number |
Filing Date |
Patent Number |
Issue Date |
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233538 |
Mar 10, 1972 |
3724747 |
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Foreign Application Priority Data
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Mar 15, 1971 [SW] |
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3309/71 |
Mar 15, 1971 [SW] |
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3310/71 |
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Current U.S.
Class: |
494/34; 494/27;
494/42 |
Current CPC
Class: |
B04B
5/0428 (20130101); B04B 2005/045 (20130101) |
Current International
Class: |
B04B
5/04 (20060101); B04B 5/00 (20060101); B04b
007/12 (); B04b 015/06 () |
Field of
Search: |
;233/2R,26,27,28,1R,3,7 |
References Cited
[Referenced By]
U.S. Patent Documents
Primary Examiner: Krizmanich; George H.
Attorney, Agent or Firm: Larson, Taylor and Hinds
Parent Case Text
This application is a division of my copending application Ser. No.
233.538, filed Mar. 10, 1972, now U.S. Pat. No. 3,724,747.
Claims
What is claimed is:
1. A container for use in treatment of liquid, particularly in
centrifugal separation of liquid into fractions of different
densities, comprising walls of flexible sheet material, the walls
defining between them a closed annular compartment for holding a
discrete quantity of liquid, said walls further defining a central
area located radially inwardly of the inner edge of the
compartment, said central area being fixed with respect to the
compartment, the walls forming said central area further defining a
collapsible conduit through which liquid may be conveyed into or
out of the container compartment, said conduit being integrally
connected with central area and hence also with the container walls
so as to be fixed relative to the walls, one end of the conduit
connected to the interior of the compartment, the conduit extending
from said one end within the central area to a further open end
also within the central area for communication with a further fluid
transporting means the fixed section of the conduit thus being
disposed and arranged to serve as the liquid conveying member of
peristaltic pump.
2. A container as set forth in claim 1 in which the conduit is
defined by heat seals joining the walls.
3. A container as set forth in claim 1 in which a connecting tube
is attached to one of the walls and communicates with the
conduit.
4. A container as set forth in claim 1 in which the walls are
generally circular and include an upper wall, a central wall and a
lower wall, the upper and central walls defining between them a
first generally annular compartment and a first conduit
communicating at one end thereof with the first compartment and at
the other end with a connecting tube secured to the upper wall, the
central and lower walls defining between them a second generally
annular compartment and a second conduit communicating at one end
thereof with the second compartment and at the other end with the
connecting tube, portions of the first and second conduits
extending along portions of respective ones of two concentric
circles and being adapted to cooperate with respective ones of two
concentric rotors of a peristaltic pump to pass liquid into and out
of the compartments.
5. A container as set forth in claim 1 in which the conduit extends
along a portion of a circular path.
6. A container for use in treatment of liquid, particularly in
centrifugal separation of liquid into fractions of different
densities, comprising walls of flexible sheet material, the walls
defining between them a closed compartment for holding a discrete
quantity of liquid and a collapsible conduit through which liquid
may be conveyed into or out of the container compartment, at least
a section of the conduit being integrally connected with the
container wall so as to be fixed relative to the walls and such
that the fixed section of the conduit is disposed and arranged to
serve as the liquid conveying member of a peristaltic pump, said
walls being generally circular and including an upper wall, a
central wall and a lower wall, the upper and central walls defining
between them a first generally annular compartment and a first
conduit communicating at one end thereof with the first compartment
and at the other end thereof with a connecting tube secured to the
upper wall, the central and lower walls defining between them a
second generally annular compartment and a second conduit
communicating at one end thereof with the second compartment and at
the other end with the connecting tube, portions of the first and
second conduits extending along portions of respective ones of two
concentric circles and being adapted to cooperate with respective
ones of two concentric rotors of a peristaltic pump to pass liquid
into and out of the compartments.
Description
This invention relates to a container for use in treatment of
liquid particularly for use in a centrifuge for treating discrete
quantities of a liquid by separating it into fractions of different
densities and, where desired, by washing soild particles suspended
in liquid. The invention has particular application to the
centrifugal treatment of blood and the present disclosure will be
devoted primarily to this application. It should be understood,
however, that the invention is applicable to the treatment of other
liquids than blood. It should also be noted that the term "liquid"
as used in this specification embraces not only true liquids but
also other materials resembling liquids such as the semi-liquid
mass of blood cells obtained from whole blood after separation of
the plasma.
It is known to treat discrete quantities of blood in a closed
system of collapsible containers in a centrifuge rotor. Thus, one
container may initially hold a batch consisting of either a mixture
of whole blood and a liquid preservative or a suspension of red
blood cells in a liquid preservative and a second container may
initially hold a quantity of a wash solution while a third
container is initially empty. The containers are interconnected
through conduits and during the treatment plasma and/or
preservative from the first container is passed into the empty
third container and temporarily replaced by wash solution from the
second container. After agitation of the contents of the first
container the used wash solution and the material washed off from
the blood cells is passed into the third container leaving the
washed red cells in the first container.
The use of collapsible closed containers for the blood, for
separated fractions and for wash solution enables the treatment to
be carried out under sterile conditions since the containers can be
interconnected in a closed system to communicate with each other
without their contents coming into contact with the ambient
atmosphere or exterior surfaces. The transfer of liquid between the
containers has presented problems, however, since the transfer
normally has to take place while the centrifuge rotor and the
container system rotate at high speed.
A general object of the present invention is to provide an improved
container construction permitting the transfer of liquid to be
effected and controlled in a simple and reliable manner.
A more specific object in accordance with the foregoing general
object is to provide a container for use in a a centrifuge in which
the rotor supports a pump for displacing liquid to and from the
container.
Another object is to provide a centrifuge which can be loaded with
the containers and made ready for operation with a minimum of
manual labor.
In one embodiment of the invention these and other objects are
realized in a container for use in a centrifuge in which the
transfer of liquid is effected by a peristaltic pump. The container
has three circular walls of flexible sheet material which walls
define between them two closed annular compartments for liquid. The
walls in the central portion, inwardly of the liquid compartments,
also define between them conduits communicating at one end with the
liquid compartments. The conduits are adapted to cooperate with the
peristaltic pump when liquid is to be transferred to or from the
liquid compartments, the peristaltic pump is caused to rotate
slowly with respect to the container to displace liquid through the
conduits. When the peristaltic pump member is stationary with
respect to the the container, it compresses the conduits to block
flow therethrough.
The above and other objects and features of the invention will
become apparent from the following detailed description taken in
conjunction with the accompanying diagrammatic drawings.
FIG. 1 is a view in vertical section of the rotor and associated
parts of a centrifuge provided with a container constructed in
accordance with the invention;
FIG. 2 is an enlarged view corresponding to the central portion of
FIG. 1;
FIG. 3 is an exploded partly cut away perspective view of the
container assembly in the centrifuge shown in FIGS. 1 and 2;
FIG. 4 is a plan view of the central portion of the two-compartment
container shown in the lower portion of FIG. 3.
The centrifuge diagrammatically illustrated in FIG. 1 has a frame
10 supporting a centrifuge rotor 11 for rotation about a vertical
axis at high speed, e.g., 3,000 rpm, by means of a motor 12. Rotor
11 includes a bowl 13 having a depending hollow journal member 14
mounted in a ball bearing 15 in frame 10. The rotor bowl and most
other elements of the rotor are circular in plan view.
Rotor bowl 13 houses a container assembly the details of which are
best seen in FIG. 3. It includes a lower two-compartment container
16 supported on the bottom wall of bowl 13 and an upper
single-compartment container 17 supported on top of container 16.
Both containers are generally disk shaped and concentric with the
rotor bowl. They are both closed, disregarding openings for the
introduction and removal of liquid, and made of a thin and flexible
sheet material so as to be collapsible. The sheet material may be,
for example, a laminate of polyethylene and polyester having a
total thickness of about 0.1 millimeter.
Lower container 16 is made of three circular sheets 18, 19, 20
disposed one on the other and sealingly joined along their
peripheries by a continuous heat seal 21 and at their central
portion by another circular heat seal 22. Lower and central sheets
18, 19 define between them a compartment 23 which initially holds
wash solution and central and upper sheets 19, 20 define between
them a compartment 24 which is initially empty.
In the central portion of container 16, heat seals 25 (marked by
closely spaced dash lines in FIG. 4) joining sheets 18, 19 define a
collapsible conduit 26 through which wash solution in compartment
23 can flow to a short connecting tube 27 secured to upper sheet 20
around an opening 28 in the latter via an opening 31 in sheet 19
(see also FIG. 2). Similar heat seals 29 (marked by closely spaced
full lines in FIG. 2) joining sheets 19, 20 define another
collapsible conduit 30 through which liquid can flow from
connecting tube 27 to compartment 24. Portions of conduits 26, 30
extend along two concentric circles and cooperate with a pump 32
described in more detail hereinafter. This pump is operable to
produce the liquid flow and to block the conduits when flow is not
desired. Heat seal 22 prevents liquid in the two compartments from
entering the central container portion except through the
conduits.
Upper container 17 initially holds a quantity of blood cells
suspended in a liquid preservative. It consists of two sheets 33,
34 which are joined by a heat seal 35 at their peripheries and a
heat seal 36 at their central portions so that they define between
them an annular compartment. A connecting tube 37 communicates with
this compartment through a conduit 38 defined by heat seals. An
opening 39 in the central portion permits tube 37 to be connected
with tube 37 of container 16.
Conduits 26 and 30 as well as conduit 38, owing to the
characteristics of the material and the manner in which they have
been produced, have a strong natural tendency to close themselves.
Thus, in order that they may permit the liquid in the containers to
pass through them, the liquid must be subjected to a substantial
pressure. Therefore, no special precautions are necessary to
prevent unwanted flow through the conduits during manual handling
of the containers.
Referring again to FIG. 1, a filler ring 40 and a backing plate 41
are disposed between containers 16 and 17. Connecting tube 27
extends through an opening in the backing plate and is connected to
connecting tube 37.
Rotor 11 includes a cover assembly with a rigid cover plate 42
which has an internally screw-threaded boss 43 and holds an annular
body 44 made of soft rubber mixed with lead granules so as to have
higher specific gravity than the liquids in the containers. A
clamping mechanism having an externally screw-threaded sleeve 45
screwed into boss 43 and a number of circumferentially distributed
wedges 46 connected to the sleeve through rods 47 cooperates with
cover plate 42 and bowl 13 to hold down the cover assembly against
the containers. A photoelectric detector 48 mounted in sleeve 45
signals the presence of red blood cells in connecting tube 37.
Pump 32 referred to above is of the well-known peristaltic type
which has a plurality of rollers moved in a circular path to
progressively collapse a resilient conduit so as to displace liquid
in the conduit. It has two concentric and independently movable
circular groups of rollers, each comprising three rollers spaced
apart 120.degree.. The outer group of rollers 49 are rotatably
mounted on an outer rotor member 50 secured to a hollow shaft 51
which is concentric with rotor 11. These rollers cooperate with
conduit 26. The inner group of rollers 52 are rotatably mounted on
an inner rotor member 53 secured to a shaft 54 extending coaxially
through shaft 51. These rollers cooperate with conduit 30.
As best seen in FIG. 2, rollers 49 and 52 engage conduits 26 and 30
through a flexible diaphragm 55 to locally compress and close these
conduits against backing plate 41.
Rotor members 50 and 53 normally are stationary with respect to the
rotating centrifuge rotor and the containers but when desired they
can be slowly rotated with respect to the centrifuge rotor during
rotation of the latter. Positive rotational movement of rotor
member 50 is derived from journal member 14 of rotor bowl 13 by
means of a gear 56 engaging a gear on the journal member and
another gear 57 engaging a gear on hollow shaft 51. Gears 56 and 57
are mounted for rotation about a common axis but normally there is
no driving connection between them. However, a magnetic clutch 58
can be actuated to cause these gears to rotate in unison so as to
bring about slow rotation of rotor member 50 with respect to
container 16 (clockwise as seen from above in FIGS. 1, 2 and in
FIG. 4). Similarly, positive rotational movement of rotor member 53
(anti-clockwise) is derived from journal member 14 through gears
59, 60 and a clutch 61.
The procedure for the treatment of the blood cells in container 17
will now be described. Rotor 11 is assumed to be stationary but
assembled as shown in FIG. 1, although compartment 24 of container
16 is empty so that sheets 19 and 20 engage each other face to face
under the influence of pressure from rubber body 44. Thus, the
peripheral portions of the containers are clamped between the
bottom of bowl 13 and filler ring 40 and between the latter and
rubber body 44. The central portions of the containers are clamped
between the rollers of pump 32 and the lower end of boss 43 of
cover plate 42. The rubber body in conjunction with the shape of
the parts ensure that unwanted air pockets adjacent the containers
are virtually eliminated.
Rotor 11 is then caused to rotate with clutches 58, 61 disengaged
so that pump rotor members 50, 53 rotate in unison with the
centrifuge rotor owing to the friction between these rotor members
and diaphragm 55 and other parts of the centrifuge rotor. Under the
influence of the centrifugal forces, the heavy soft rubber of body
44 is forced outwardly to apply an external pressure to containers
16, 17. Owing to the arrangement and shape of the parts, this
pressure forces the liquid in the containers inwardly and causes
conduits 26, 30 to assume the expanded form shown in FIG. 2.
However, since the rollers of the rotor members are stationary with
respect to the rotor and the containers and compress the conduits,
no liquid is permitted to pass through the latter.
The centrifugal field, which may be of the order of 1,000 g, causes
the formation of fractions of different densities in container 17,
that is, the red blood cells accumulate in the radially outer
portion of container 17 while the lighter preservative liquid is
collected in the radially inner portion. Clutch 61 is then engaged
to cause inner rotor member 53 to rotate anticlockwise (FIG. 4)
with respect to the centrifuge rotor and the containers so that the
preservative liquid is pumped from container 17 into compartment 24
of container 16 through conduit 38, connecting tubes 37, 27 and
conduit 30. Since outer rotor member 50 is still stationary with
respect to the centrifuge rotor and the containers, the
preservative liquid is prevented from flowing through conduit
26.
When detector 48 signals the presence of red blood cells in tube
37, clutch 61 is again disengaged and clutch 58 engaged so that
outer rotor member 50 is caused to rotate to pump wash solution
from compartment 23 into container 17 through conduit 26. tubes 27,
37 and conduit 38 while inner rotor member 53 is held stationary to
prevent flow through conduit 30. When a sufficient amount of wash
solution has been transferred, clutch 58 is disengaged so that both
conduits 26, 30 are closed whereupon rotor 11 is rapidly braked (by
means not shown) to low speed to agitate the contents of container
17 and thoroughly mix the wash solution and blood cells.
The wash solution is then separated from the blood cells and
transferred to compartment 24 of container 16 in the same manner as
has been described for the preservative liquid. The washing step
described above is repeated as many times as necessary and when the
treatment is completed, container 17 contains a concentrate of
washed blood cells while container 16 contains liquid preservative
and used wash solution in compartment 24. Compartment 23 may be
empty or contain a residue of wash solution.
It will be appreciated that the described two-compartment container
with its integral conduits offer significant advantages from a
manufacturing as well as from a handling point of view.
* * * * *