U.S. patent number 3,679,128 [Application Number 05/061,583] was granted by the patent office on 1972-07-25 for centrifuge.
This patent grant is currently assigned to AGA Aktiebolag. Invention is credited to Gunnar Ingemar Berndtsson, Stephen Lars Schwartz, Hans Peter Olof Unger, Johan E. H. Westberg.
United States Patent |
3,679,128 |
Unger , et al. |
July 25, 1972 |
CENTRIFUGE
Abstract
A centrifuge comprises a rotating chamber containing a
compressible container for the liquid to be centrifuged. The
centrifuge also includes means to compress the container to
transfer liquid from the container during centrifugation. The
compressing means is also capable of compressing one or more
further compressible containers to transfer liquid therefrom to the
first-mentioned compressible container. The compressing means is
preferably a diaphragm member which is urged by centrifugal force
against a compressible container. In one application, the container
is filled with blood to be centrifuged and, after the serum is
separated, a valve is opened permitting the diaphragm to compress
the container to transfer the serum to a further container. The
valve is then closed and speed reduced until the diaphragm returns
to its rest position causing compression of a still further
container provided with wash water. A valve is then opened to
permit transfer of some or all of the wash water into the
first-mentioned container and the device may then be braked to
cause the water to mix with the blood cells therein. The washed
blood cells are then centrifuged and the water transferred to the
water container or yet a still further container by the actuation
of an appropriate valve similarly as in the case of transferring
the serum. The washing operation is repeated if desired until all
of the wash water has been utilized.
Inventors: |
Unger; Hans Peter Olof
(Stockholm, SW), Westberg; Johan E. H. (Lidingo,
SW), Berndtsson; Gunnar Ingemar (Stockholm,
SW), Schwartz; Stephen Lars (Lidingo, SW) |
Assignee: |
AGA Aktiebolag (Lidingo,
SW)
|
Family
ID: |
20293390 |
Appl.
No.: |
05/061,583 |
Filed: |
August 6, 1970 |
Foreign Application Priority Data
|
|
|
|
|
Aug 11, 1969 [SW] |
|
|
11121/69 |
|
Current U.S.
Class: |
494/85;
494/32 |
Current CPC
Class: |
B04B
5/0428 (20130101) |
Current International
Class: |
B04B
5/00 (20060101); B04B 5/04 (20060101); B04b
011/00 () |
Field of
Search: |
;233/26,27,28,29,35,47R,2R,9,1R ;417/294,395 |
References Cited
[Referenced By]
U.S. Patent Documents
Primary Examiner: Franklin; Jordan
Assistant Examiner: Krizmanich; George H.
Claims
What is claimed is:
1. A centrifuge comprising:
a rotor;
a first chamber mounted for rotation with said rotor;
a first compressible container located in said chamber;
a second chamber mounted for rotation with said rotor;
at least one further compressible container located in said second
chamber;
passage means placing said first compressible container in fluid
communication with said further compressible container;
valve means for controlling the flow of fluid through said passage
means;
a first diaphragm for compressing said one flexible container in
said one chamber;
a second diaphragm spaced from said first diaphragm and including a
cavity therebetween;
said cavity between said diaphragms being filled with a
displacement fluid; and
said flexible diaphragms being inclined with respect to the axis of
rotation of said rotor such that, at a certain rotational speed,
said diaphragms are urged from a rest position to a high speed
position compressing said one compressible container.
2. A centrifuge according to claim 1 wherein said cavity includes a
rigid wall positioned between said diaphragms, said wall including
an opening therethrough for the passage of said displacement
fluid.
3. A centrifuge according to claim 2 wherein said diaphragms are
downwardly curved in said rest position and upwardly curved in said
high speed position.
4. A centrifuge according to claim 1 including a plurality of
further compressible containers each in fluid communication with
said first compressible container through said passage means, said
valve means controlling the fluid flow between said first
compressible container and each of said further compressible
containers.
5. A centrifuge according to claim 4 wherein said plurality of
further compressible containers are located in said second
chamber.
6. A centrifuge according to claim 5 wherein said first container
includes a liquid to be centrifuged, and said further compressible
containers comprise a container for receiving a phase separated
from the centrifugal liquid, a container for wash liquid to be
introduced into said first container after separation of said
phase, and a container for receiving wash liquid from said first
container, said centrifuge including means for arresting motion of
said first chamber whereby said wash liquid is mixed with the phase
in said first container.
Description
BACKGROUND OF THE INVENTION
The invention relates to a centrifuge and particularly to a
centrifuge suitable for the treatment of blood.
In the treatment of blood the problem often arises of separating
blood corpuscles from serum or replacing the serum by some other
liquid. In the latter case a careful washing of the blood
corpuscles may become necessary so as to remove as completely as
possible the blood serum from the same.
It is known that blood can be treated in a cylindrical centrifugal
rotor in which is arranged an equally cylindrical filter, the blood
being on the inside (centrally) around the filter wall and the
washing liquor being outside the same in the rotor chamber. During
centrifuging, the liquid flows towards the center and the blood
corpuscles are maintained in suspension by suitable adjustment of
the centrifugal force in relation to the flow of the washing
liquor. This device is bulky and clumsy and relatively complicated,
which renders it difficult to maintain sterility. The device is
also expensive.
It is an object of the present invention to provide a centrifuge
which is free of the disadvantages mentioned above. It is a further
object to provide a simple and inexpensive centrifuge allowing
separation of blood during centrifuging while maintaining sterile
conditions.
BRIEF SUMMARY OF THE INVENTION
The foregoing and other objects which will become apparent to those
having ordinary skill in the art are achieved according to the
present invention by providing a centrifuge comprising a rotor, a
chamber mounted for rotation with the rotor, a compressible
container located in said chamber, passage means in fluid
communication with said chamber, valve means for controlling fluid
flow through said passage means, and means for compressing said
flexible container to decrease the volume thereof.
DETAILED DESCRIPTION
There follows a detailed description of a preferred embodiment of
the invention, together with accompanying drawings. However, it is
to be understood that the detailed description and accompanying
drawings are provided solely for the purpose of illustrating a
preferred embodiment and that the invention is capable of numerous
modifications and variations apparent to those skilled in the art
without departing from the spirit and scope of the invention.
FIG. 1 is a radial elevation section along line I in FIG. 2 through
the top portion of a centrifuge according to the invention;
FIG. 2 is a schematic perspective view of the central portion of
the centrifuge of FIG. 1 showing the course of conduits;
FIG. 3 is a schematic expanded perspective view showing connections
between containers provided in the centrifuge of FIG. 1; and
FIG. 4 is a diagram illustrating the control of fluid flow between
the containers.
FIG. 1 is a radial section of a centrifuge according to the
invention to which correspond similar sections at 120.degree. and
240.degree. distance, as can be seen in FIG. 2. On the central
portion 1 of the centrifuge rotor is fitted a bottom disc 2 and on
this an outer ring 3, which has inside it three rectangular threads
with three entrances. A top disc 4 is fixed on a threaded inner
ring 5, whose threads match those of the outer ring 3. The outer
volume of the centrifuge formed in this manner is divided into two
chambers by a control device 6. This has a rigid conical partition
wall 7 which is penetrated by an opening 8. On both sides of the
partition wall 7 are situated clamped rubber diaphragms 9 and 10
and the diaphragm 10 is reinforced on the underside by a further
rubber diaphragm 11 vulcanized to it which is heavy and has a high
specific gravity. The diaphragms 9 and 10 are strongly biassed, so
that they are urged to be curved downards under their own stress as
shown in FIG. 1. Enclosed between the diaphragms 9 and 10 is a
cavity 12 containing a displacement liquid having a volume equal to
the liquid quantity which will be conveyed to or from the blood
being centrifuged as will be explained below. Details concerning a
suitable control device are described in U.S. application Ser. No.
61,573, filed Aug. 6, 1970, entitled "Rotating Sensitive Pumping
Device," herein incorporated by reference. The control device can
be raised and lowered within the centrifuge rotor through the
presence on it of three plugs 13 which fit into the threads of the
ring 3 and can so be adjusted up and down by twisting.
In the space between the bottom disc 2 and the control device 6 are
three compressible containers 14, 15, 16. A compressible blood
container 17 is arranged between the top disc 4 and the control
device 6. The container 17 is covered by a conforming plastic disc
18 with a number of ridges 19. The compressible containers are
annular in shape and conveniently made of polyethylene or other
plastic film inert to blood. Details of a preferred bag are
disclosed in U.S. application Ser. No. 61,574, filed Aug. 6, 1970
titled "Container for Blood" herein incorporated by reference.
A screen 20 surrounds a narrower part of the central portion 1 of
the centrifuge rotor. A slot 21 is provided in screen 20 through
which flexible tubes 14', 15', 16' connected to the containers 14,
15, 16 are introduced into the space between rotor 1 and the screen
20. Each tube 14', 15', 16' connects its respective container with
container 17 and each is provided with a valve 14", 15", and 16" to
control the flow of fluid between container 17 and each container
14, 15, and 16.
Valve 16" is visible in FIG. 1 and comprises an electro magnet 22
which acts on a valve arm 23, which forms a loop in which is
inserted the line 16', so that the line is closed when the magnet
is activated. Electromagnet 22 is actuated by electrical leads 22'
in a conventional manner. When current is broken, line 16' is free,
and the release of the line can be assisted by a spring 22". These
details are conventional and can obviously be varied in a number of
different ways.
FIG. 4 shows schematically how a program register 24 is arranged
for the control of the operation of the centrifuge 25 through lines
to the valves 14", 15", 16" and to a brake 26, which may be any
suitable electrically controlled brake. It should be noted that the
valves 14", 15", 16" are opened when the magnets are
currentless.
The separation of blood plasma from blood corpuscles and washing of
the latter takes place in the following manner:
It is assumed that the centrifuge is intended for five washings of
a quantity of blood, for example 250 ml. Container 14 is filled
with 5 .times. 250 = 1,250 ml of washing liquor. Containers 15 and
16 are empty. Containers 14, 15, and 16 are placed in the lower
chamber of the centrifuge on the bottom disc 2, as can be seen in
FIG. 1. The three connecting lines or flexible tubes are placed
around the central portion 1, as shown in FIG. 2 and clamped tight
at the valve devices 14", 15", 16".
Control device 6 is now threaded in until practically all the air
has been driven out from the lower centrifuge space. Control device
6 has then reached its bottom position, and the displacement fluid
(250 ml) in cavity 12 is below partition wall 7. Owing to the
substantial diaphragm stress it is not possible by hand to press
the displacement fluid up through the opening 8. Control device 6
is locked in this position by any suitable known device, for
example, a locking screw, which is not shown in the drawings.
Subsequently, the blood container 17 and the disc 18 are inserted,
whereupon the three connecting lines to container 17 are joined
together with three lines from the containers 14, 15, 16 as shown
in FIG. 3. The top disc 4 of the centrifugal rotor with ring 5 is
screwed down into position as shown and locked in a suitable
manner.
The centrifuge can now be started, and approximately three minutes
after it has reached the steady state with approximately 1,000 g in
the periphery, the blood corpuscles have been packed together and
the plasma, which constitutes approximately half the volume, can be
conveyed to empty container 16. Through the program register 24
valve 16" is opened. Thus, a connection is established between the
top and bottom space of the centrifuge. The heavy diaphragm 10,
whose centrifugal force is greater than the diaphragm stresses,
drives the displacement fluid through the opening 8. Through this,
a corresponding amount of plasma is driven from container 17 into
container 16. When the conveying has been completed, the program
register closes valve 16" and opens valve 14", so that a connection
is established between the containers 17 and 14. At the same time
the rotational speed of the centrifuge is lowered to a low value
such that the stresses in the rubber diaphragm become substantially
greater than the centrifugal force of the heavy diaphragm.
Therefore, a pumping action in the opposite direction sets in, and
container 17 is filled up again with 250 ml washing liquor from
container 14 owing to the displacement fluid being driven down
again through the opening 8. When container 17 is filled, valve 14"
is closed and the rotational speed of rotor 1 is increased. When
the operating speed has been attained, the motor is stopped and the
centrifuge is quickly braked by the brake 26 but the liquid in the
blood container 17 continues to rotate. The container, which is
flexible, has in the meantime engaged projections 19 which through
this provide a stirring action in container 17 such that an
effective mixing of the blood corpuscles with the washing liquor is
obtained.
The brake is now released and the centrifuge is re-started.
Approximately 3 minutes after the steady state has been attained,
the blood corpuscles have again been packed together and the liquor
can be tapped. This is done by opening valve 15", so that the
consumed washing liquor is conveyed to the container 15 in the same
manner as described above for the transport of plasma to container
16. The washing process is repeated five times whereupon the
centrifuge is stopped.
The result is that the blood corpuscles have been thoroughly washed
and a minimum of washing liquor remains in the blood container 17.
The plasma has been conveyed to the plasma container 16 and the
washing liquor to the discharge container 14. The displacement
fluid which at the beginning was in the lower part of control
device 16 is now in the top part thereof and the bottom part is
consequently exposed to a substantial overpressure caused by the
diaphragm stresses. After the stop screws have been loosened, the
top part 4, 5 is screwed off. The blood container 17 is detached
from the connecting lines and the plastic disc 18 is taken off. The
connecting tube of the discharge container 15 is connected up to a
discharge and the valve 15" is opened. As a result, 250 ml consumed
washing liquor runs off, so that the displacement fluid drops down
into the bottom part. The overpressure in the bottom part
disappears, and the centrifuge can be dismantled.
It will be apparent that without any other change of the centrifuge
than coupling to a different program and a different content in the
container 17, it may also be used for washing out protecting agent
from frozen blood and conversion of the blood to a condition
suitable for transfusion.
According to the modified embodiment, the function of control
device 6 may be exercised by means of a pressure variation across
the control device brought about, for example, by sealing off of
the chamber where the blood container is situated and supply of the
pumping pressure to that chamber via a rotating coupling.
Such a centrifuge operates in the same manner as the one described
above, except that at least one of the chambers of the centrifuge
must be in airtight separation from the atmosphere environment as
well as from the other chamber. The pumping action from the one
chamber to the other chamber is not obtained through variation of
speed, but through variation of the air pressure in the airtight
chamber surrounding the containers. The amount of liquid which is
transported is determined in this variant by the pressure set up
when, at a certain speed, equilibrium can permanently be made to
prevail at a certain level, through the application of a certain
external overpressure.
Centrifuges in accordance with the invention are intended in the
first place to be used for the treatment of one blood unit at a
time, but it will be apparent that it is quite possible to stack on
the same axle a number of compressible containers.
* * * * *