U.S. patent number 3,879,295 [Application Number 05/389,275] was granted by the patent office on 1975-04-22 for vacutainer with positive separation barrier.
This patent grant is currently assigned to Eastman Kodak Company. Invention is credited to Clyde P. Glover, Michael P. O'Neill.
United States Patent |
3,879,295 |
Glover , et al. |
April 22, 1975 |
**Please see images for:
( Certificate of Correction ) ** |
Vacutainer with positive separation barrier
Abstract
Apparatus providing a physical barrier between the phases of a
two-phase liquid, such as whole blood stratified into a serum phase
and a cells phase, which includes a transparent tubular receptacle
having a rigid wall capable of holding a vacuum. The receptacle has
an internal constriction for receiving a resilient plug, which
divides the receptacle into an upper chamber and a lower chamber.
In the preferred embodiment, the plug has a lower portion, a neck
portion and an upper portion. The end of the lower portion is
shaped to provide an open pocket for holding a volume of air. A rod
of suitable length is detachably mounted through the upper portion
of the plug to enable one to urge the plug through the serum in the
upper chamber and in sealing engagement with the constriction. The
air entrapped in the pocket at the lower portion of the plug
creates a slight increase in pressure in the lower chamber as the
plug is deformably urged into sealing engagement with the
constriction thereby sealingly urging the lower portion of the plug
against the constriction. The rod is thereafter detached from the
plug and the serum may be asperated out or decanted for analysis,
or the open end of the receptacle may be sealed with a stopper
thereby making the receptacle ready for use, storage or
transportation.
Inventors: |
Glover; Clyde P. (Rochester,
NY), O'Neill; Michael P. (Rochester, NY) |
Assignee: |
Eastman Kodak Company
(Rochester, NY)
|
Family
ID: |
23537582 |
Appl.
No.: |
05/389,275 |
Filed: |
August 17, 1973 |
Current U.S.
Class: |
210/516;
210/789 |
Current CPC
Class: |
A61B
5/15003 (20130101); B01L 3/5021 (20130101); A61J
1/20 (20130101); A61B 5/154 (20130101); A61B
5/150351 (20130101); A61B 5/150755 (20130101); A61B
5/150251 (20130101); B01L 2400/0633 (20130101) |
Current International
Class: |
A61J
1/00 (20060101); A61B 5/15 (20060101); B01d
021/26 () |
Field of
Search: |
;210/DIG.23,DIG.24,516-518,443,446,361,359,83,84 ;233/2,2B,26,1A,1R
;23/259 ;128/214R,2F,272,218M |
References Cited
[Referenced By]
U.S. Patent Documents
Primary Examiner: Hart; Charles N.
Assistant Examiner: Calvetti; F. F.
Attorney, Agent or Firm: Schmidt; D. M.
Claims
We claim:
1. Apparatus for separating with a physical barrier one phase from
the remainder of a liquid having at least two phases,
comprising:
a. a tubular unitary receptacle open at one end and closed at the
opposite said one end, for containing such liquid,
said receptacle having an internal constriction defining an
aperture which divides said receptacle into a first chamber for
containing such one phase only and a second chamber for containing
the remainder of such liquid,
said aperture having a cross-sectional dimension smaller than the
smallest internal cross-sectional dimension of said first
chamber;
b. a resilient plug for sealingly engaging said constriction to
form a physical barrier between said first chamber and said second
chamber,
said plug being symmetrical about a centerline and smaller in
cross-section at its widest diameter than the largest internal
cross-sectional dimension of said first chamber, and having a first
portion, a neck portion, and a second portion for engaging said
constriction at, respectively, said first chamber, said aperture,
and said second chamber; and
c. rod means detachably mounted through said first portion of said
plug in coincidence with said centerline for urging said plug into
sealing engagement with said constriction, said rod means being
thereafter removable from said plug.
2. The invention as defined in claim 1 wherein:
said second portion of said plug has an undeformed cross-section at
its widest diameter larger than such cross-sectional dimension of
said aperture, and a configuration operatively related to said
constriction for guiding said second portion through said aperture
and into said second chamber in response to a force applied through
said rod means,
said neck portion of said plug has an undeformed cross-section
slightly larger than such cross-sectional dimension of said
aperture and being compressable for sealing engagement with said
constriction at said aperture as said second portion is urged into
place in said second chamber, and
said first portion of said plug has a larger cross-section than
said neck portion for maintaining said neck portion in sealing
engagement with said constriction.
3. The invention as defined in claim 2 wherein said second portion
of said plug includes an open pocket at the end remote from said
neck portion for receiving a volume of air and retaining said air
as said plug is urged through such one phase of such liquid and as
said second portion is deformingly urged through said
constriction.
4. In an apparatus for maintaining separation by a physical barrier
of a stratified two-phase liquid, such as a blood specimen
following stratification of the heavier cells from the lighter
fluid, of the type having a tubular unitary receptacle open at one
end and closed at the end opposite said one end, and a resilient
plug forming said physical barrier, the improvement comprising:
an internal constriction in said receptacle defining an aperture
which divides said receptacle into a first chamber and a second
chamber, and which is of smaller cross-sectional dimension than the
smallest internal cross-sectional dimension of said first chamber,
for receiving said resilient plug in sealing engagement therewith
to form said physical barrier separating the lighter fluid in said
first chamber from the remaining liquid, which includes said
heavier cells, in said second chamber;
said plug having a first portion, a second portion, and a neck
portion between said first and second portions for engaging said
constriction at, respectively, said first chamber, said second
chamber, and said aperture.
5. The invention as defined in claim 4 wherein said second chamber
comprises about 55 percent of the operatively usable volume of said
tubular receptacle.
6. In an apparatus for maintaining the separation by a physical
barrier of a stratified two-phase liquid, such as a blood specimen
following stratification of the heavier cells from the lighter
fluid, of the type having a tubular receptacle, open at one end and
closed at the end opposite said one end, with an internal
constriction defining an aperture which divides said receptacle
into a first chamber and a second chamber, the improvement
comprising:
a. a resilient plug for sealingly engaging said constriction to
form said physical barrier between said first chamber and said
second chamber,
said resilient plug being symmetrical about a centerline and having
a first portion, a neck portion, and a second portion for engaging
said constriction at, respectively, said first chamber, said
aperture, and said second chamber; and
b. rod means detachably mounted through said upper portion in
coincidence with said centerline for urging said plug into sealing
engagement with said constriction, said rod means being thereafter
removable from said plug.
7. The invention as defined in claim 6, wherein said second portion
of said resilient plug includes an open pocket at the end remote
from said neck portion for receiving a volume of air and retaining
said air as said plug is urged through such lighter fluid of such
liquid and as said second portion is urged through said
constriction.
Description
BACKGROUND OF THE INVENTION
1. Field of the Invention
This invention relates generally to apparatus for the physical
separation of one phase from the remainder of a liquid having at
least two phases. More specifically, it relates to the separation
by a physical barrier of blood serum from blood cells after the
blood has been stratified, e.g., by centrifugation, in a tubular
receptacle.
2. Description of the Prior Art
The analysis of blood serum or plasma, extracted from whole blood,
is an invaluable tool to the medical profession in diagnosing and
treating a multiplicity of human disorders and diseases. The
separation of this blood serum or plasma from whole blood is an
important step in arriving at a correct medical analysis from which
proper medication or treatment can be prescribed. Currently, a
specimen of serum or plasma is obtained by injecting one end of a
hollow needle, open at both ends, into an appropriate vein of the
donor followed by injection of the other end of the needle into an
evacuated tube through a rubber stopper capable of holding a
vacuum; the negative pressure in the evacuated tube causes whole
blood to flow from the vein into the tube. Ordinarily, such
containers are designed to collect approximately 10 milliliters of
blood, although there are also containers designed for collecting 5
milliliters of blood.
Once a specimen of blood has been collected in the stoppered
container, if serum is the end product desired for analysis, the
technician will remove the stopper and add a clotting agent to the
specimen or let the blood stand for some period of time (around
one-half hour) to allow the blood to clot. The blood thereafter
undergoes centrifugation in the same container to separate the
lighter serum from the heavier cells. If plasma is the desired end
product for analysis, an anticoagulation agent is added to the
specimen or it is centrifuged immediately upon collection, before
appreciable coagulation occurs. In either case, the result of
centrifugation is the stratification of blood into a lighter phase
and a heavier phase. Since the more usual laboratory practice in
blood analysis is to work with serum rather than plasma, the
lighter phase of the stratified blood will be assumed to be serum,
although it is understood that it could equally be plasma.
Following stratification of the blood into serum and cells, the
serum is isolated from the cells by being asperated or decanted
from the first container into a second container. Isolation of the
serum is desirable to prevent possible chemical or physical
interaction, between the two interfaced phases, during storage or
transportation which could lead to erroneous results in the
analysis. Since some turbulance is normally unavoidable in the
process of transferring the serum, a second centrifugation may
follow to collect the remaining cells at the bottom of the second
container.
The aforementioned procedure in obtaining serum obviously involves
a number of time consuming steps. More significantly, however, the
transfer of the serum from the first container to the second
increases the risk of contamination, spillage, and loss of identity
of the donor. Therefore, an improved device for separating serum
from cells would preferably embody or provide a number of desirable
attributes, e.g., (1) obtaining a sample of blood and achieving
separation of the two phases under sterile conditions; (2)
minimizing the risk of loss of identity of the donor of the blood
throughout the entire clinical process; (3) minimizing the
migration of cells once the blood has been stratified into cells
and serum; (4) utilizing, storing, or transporting the serum
without interplay between the serum and the cells; (5) economic
feasibility in manufacturing a disposable device; (6) the ability
to physically separate the container at a particular location
determinable by the purpose of the test; (7) rapidity and
simplicity in inserting the physical barrier to separate the serum
from the cells.
Recently, some developments in the art have provided devices having
some of these attributes. Two such examples are found in U.S. Pat.
Nos. 3,508,653 to Coleman and 3,647,070 to Adler. The Coleman
patent discloses a method and apparatus for urging a piston through
the lighter upper phase, to the interface between the serum and the
cells. The container in the Coleman patent is a tubular receptacle
of regular cross-section. The piston is formed of resilient
material and has an undeformed cross-section slightly larger than
the inner diameter of the tubular receptacle. To position the
piston at the interface of the two phases, a force is applied,
either by pushing the piston with a rod or through centrifugation,
which causes the piston to move toward the closed end of the
receptacle while the fluid pressure of the light phase causes the
piston to be deformed sufficiently to allow the liquid to flow
around the piston toward the open end of the receptacle. The force
is removed when the piston reaches the interface between the light
phase and the heavy phase of the two-phase blood.
The Adler patent also discloses a device in which a physical
barrier is formed in a tubular container of regular cross-section
at the interface between the lighter phase and the heavier phase of
a two-phase liquid. Prior to insertion, the barrier has a
cross-section smaller than the inner diameter of the tubular
container. It has a specific gravity greater than the heavier phase
but less than the lighter phase so barrier sinks through the
lighter phase until it reaches the interface of the two phases.
Thereafter, through chemical reaction with the serum, the barrier
expands to form a seal. Also disclosed in the Adler patent is a
two-part container assembly including a tube which has a sleeve
insertable therein. The sleeve is generally shaped as a hopper,
i.e., it terminates in a generally truncated cone-shaped end
portion which has external threads for receiving a like-threaded
cap. To physically separate the cells from the serum, a plug is
provided which is fixedly attached to one end of a rod; the other
end of the rod is fixedly attached to the inner portion of the
stopper. The length of the rod is such that when the stopper is
inserted into the sleeve, the plug is forced into the truncated
opening at the bottom of the sleeve thereby providing a physical
barrier between the serum in the sleeve and the cells in the tube.
The sleeve is then removed from the tube and the threaded cap is
screwed into the truncated end.
Although the art has been advanced by the Coleman and Adler
patents, there remains an opportunity for improvement. For example,
in the Coleman patent due to the configuration of the piston, a
large force, either centrifugal or direct action as with a rod,
must be applied in order to deform the piston so that the serum
will flow around it. This may entail the necessity of obtaining new
centrifugation equipment to comply with the added force
requirement. In the case of using a detached rod to push the
piston, problems may arise in aligning the rod with the center of
the piston to avoid unequal forces in the piston, as well as in
applying the proper deforming force to avoid possible contaminating
spillage, or turbulance which can disturb the compacted cells and
contaminate the serum. The chemically activated barrier formed in
the Adler patent has the disadvantage that it is designed to be
formed at the interface between the light and heavy phase of a
two-phase liquid having a particular density differential. For
example, if for a particular purpose, such as to save time, only a
small amount of serum is required relative to the amount of whole
blood, the density change in the heavier phase may be insufficient
to buoy the barrier at the interface. Moreover, there is a waiting
period in which the physical barrier expands to form the seal at
the interface. That is, the container must be held in place until
the barrier expands to form the seal. This may inconvenience the
technician and add valuable time to the test schedule. Since it is
made up of numerous parts, the two-part container disclosed by
Adler has the disadvantages of complexity, expense and a
multiplicity of handling operations.
Regarding the desirable attributes enumerated above, it is clear
that the prior art does not disclose or provide a device having all
of them.
Accordingly, it is an object of the invention to provide a
disposable receptacle for containing blood stratified into a
lighter phase and a heavier phase wherein a physical barrier can be
inserted rapidly and easily to separate the lighter phase from the
remaining liquid.
It is another object of the invention to provide a device for
physically separating blood serum from the cells wherein no
migration of the cells or contamination of the serum occurs once
the physical barrier is positioned in place.
It is another object of the present invention to provide a
disposable receptacle for the separation of serum from cells which
is economically feasible and simple to manufacture.
It is another object of the invention to provide a disposable
receptacle for the separation of serum from cells in which the
location of the constriction receiving the physical barrier can be
varied in manufacture according to the purpose of the test.
SUMMARY OF THE INVENTION
These and other objects are accomplished according to the preferred
embodiment of the present invention by forming a constriction, for
sealingly receiving a plug, in a tubular receptacle used in
containing a specimen of blood from a donor. The constriction
defines an aperture which divides the receptacle into a lower
chamber and an upper chamber, and which has a crosssectional
dimension smaller than the smallest interior crosssectional
dimension of the upper chamber of the receptacle. After
centrifugation of the blood, the cells are compacted into the lower
chamber while the serum remains in the upper chamber. A plug is
then deformingly inserted into the constriction thereby placing a
physical barrier between the lower chamber and the upper chamber.
The resilient plug is symmetrical about a centerline and at its
widest diameter is smaller in cross-section than the largest
internal cross-sectional dimension of the upper chamber. The plug
has a lower portion, a neck portion and an upper portion for
sealingly engaging the constriction at, respectively, the lower
chamber, the aperture, and the upper chamber. Detachably mounted to
the upper portion of the plug, coincident with the centerline
thereof, is a rod of operative length for urging the plug into
sealing engagement with the constriction, the rod being thereafter
removable from the plug.
In one embodiment of the invention, the lower portion of the plug
has an undeformed cross-section larger than the aperture of the
constriction and a configuration operatively related to the
constriction for guiding the lower portion of the plug through the
aperture and into the lower chamber in response to a plug deforming
force applied through the rod. The neck portion has a cross-section
slightly larger than the aperture and is compressed into sealing
engagement with the constriction as the lower portion is urged into
place in the lower chamber. The upper portion of the plug has a
larger cross-section than the neck portion for maintaining the neck
portion in sealing engagement with the constriction. The sealing
properties of the plug can be further improved by forming an open
pocket at the end of the lower portion to receive a volume of air
and retain it as the plug is urged through the serum and as the
lower portion is deformingly urged through the constriction. The
effect of the trapped air is to increase slightly the pressure in
the lower chamber thereby sealingly urging the lower portion of the
plug against the constriction.
The invention and its objects and advantages will become more
apparent in the detailed description of the preferred embodiment
presented below.
BRIEF DESCRIPTION OF THE DRAWINGS
In the detailed description of the preferred embodiment of the
invention presented below, reference is made to the accompanying
drawings in which:
FIG. 1 is an isometric view of the preferred embodiment of the
invention showing a tubular receptacle having a constriction and a
resilient plug with a detachable handle;
FIG. 2 is an enlarged longitudinal cross-sectional view of the
constriction, the other portions of the receptacle cut-away for
clarity;
FIG. 3 is a longitudinal cross-sectional view of the plug of the
preferred embodiment showing a break-away rod and an open pocket at
the lower portion of the plug;
FIG. 4 is a cross-sectional view of the receptacle of the invention
following the collection of a specimen of whole blood;
FIG. 5 is a longitudinal cross-sectional view of the receptacle
after the whole blood has been subjected to centrifugation and the
plug is about to be inserted in the constriction to effect physical
separation;
FIG. 6 is an enlarged view of the constriction and plug shown in
FIG. 5 also showing the deformation of the lower portion of the
plug as the plug is urged into sealing engagement with the
constriction;
FIG. 7 is a longitudinal cross-sectional view of the receptacle
showing the plug in sealing engagement with the receptacle and the
handle being detached from the plug thereby effecting physical
separation between the cells in the lower chamber and the serum in
the upper chamber;and
FIG. 8 is a longitudinal cross-sectional view of another embodiment
of the plug showing a pull-away rod.
DESCRIPTION OF THE PREFERRED EMBODIMENT
Referring now to the drawings wherein like referenced numerals have
been used in several views and figures for like elements, FIG. 1
illustrates a tubular receptacle generally indicated by reference
numeral 10 and a resilient plug generally indicated by reference
numeral 30, having rod means 35. The receptacle is integrally
formed with a lower closed end 14 and an upper open end 13. "Upper"
and "lower" are used throughout this specification in relation to a
tubular receptacle which is parallel to a gravitational vector. The
constriction 20 formed in receptacle 10 divides the receptacle into
an upper chamber 11 and a lower chamber 12. It is common practice
in blood analysis to draw approximately 10 milliliters of blood
from a donor. Receptacle 10 is designed to accept approximately 10
milliliters of blood and the constriction is positioned such that
lower chamber 12 has approximately 55 percent of the usable volume
and upper chamber 11 has the remaining 45 percent. After
centrifugation, the compacted cells normally make up slightly over
50 percent of the volume. Hence, all the cells will be in the lower
chamber 12 leaving only serum in the upper chamber 11. If
appropriate, e.g., where a small amount of serum is needed relative
to the total blood specimen, the relative volume in upper chamber
11 may be reduced by forming constriction 20 closer to open end
13.
The tubular receptacle can be made from a variety of materials by a
variety of manufacturing techniques, both materials and techniques
being well known in the art and neither forming any part of the
present invention. For example, it can be made economically from
synthetic resins such as Plexiglass by, e.g., injection
blow-molding techniques. Structurally, the receptacle must be
capable of withstanding the pressure differential on the walls
resulting from the evacuation of air from the interior; chemically,
it must not appreciably react with the blood; esthetically, it is
usually made of a transparent material. A tubular receptacle made
of, e.g., Plexiglass would satisfy these requirements since the
tubular design would give it the structural strength required, the
molding material does not react appreciably with blood, and it is
transparent. However, a material of substantially comparable
characteristics would serve as well.
The plug 30 can be formed from a variety of materials, e.g.,
rubber, which are resilient and do not interact chemically with
serum or plasma. It is clear that constriction 20 may assume a
variety of shapes so long as plug 30, which provides a barrier at
the constriction, is formed in operative relation to the shape of
the constriction. For example, there should be an obvious
relationship between the shape and size of the constriction at the
aperture and that of the plug at the sealing area; similarly, the
shape of the constriction in the upper chamber and the end of the
plug which first comes in contact with the constriction should be
so related that the plug can be mechanically guided into place. As
seen more clearly in FIG. 3, plug 30 in the preferred embodiment
includes a lower portion 31 having an open pocket 34, a neck
portion 32 and an upper portion 33 from which detachable rod 35
protrudes. The toe 38 of rod 35 may be located in upper portion 33,
as shown in FIG. 3, or it may extend through the upper portion and
into the lower portion 31, above open pocket 34 (not shown). The
latter location would enable lower portion 31 to stretch in
response to a force transmitted through rod 35, thereby
facilitating insertion of plug 30 into constriction 20. Ordinarily,
however, the location of the toe 38 is determined by such factors
as the resilience of the plug, the relative dimensions and
configurations of the plug and the constriction, and the manner in
which the rod is to be detached from the plug.
In upper chamber 11, constriction 20, terminating in cylindrical
aperture 24, flares conically from aperture 24 to meet the interior
cylindrical wall 26 of upper chamber 11. In lower chamber 12, the
constriction steps from cylindrical aperture 24 to cylindrical bore
22 whose cross-sectional dimension is greater than that of aperture
24 but ordinarily less than that of the interior cylindrical wall
26 of lower chamber 12. The cross-sectional dimension of
cylindrical bore 22 is slightly less than that of the largest
cross-section of lower portion 31 of plug 30 so that lower portion
31, by being slightly compressed, fits snugly in the lower chamber.
The annular wall 29 defined by the difference between cylindrical
aperture 24 and cylindrical bore 22 is sealingly engaged by annular
shoulder 39 of plug 30 defined by the difference in cross-section
between neck portion 32 and lower portion 31. See FIG. 3. Following
cylindrical chamber 22, the constriction flares at 23 to meet
interior cylindrical wall 26 of lower chamber 12.
FIG. 4 is a view of the receptacle filled with approximately 10
milliliters of whole blood from a donor. The method for drawing
blood is well known in the art and will not be described in great
detail. Ordinarily the empty receptacle has a resilient stopper 15
which is capable of holding a vacuum. One end of a double-ended
needle is injected into a donor and the other end is injected into
the evacuated receptacle 10 through stopper 15. The negative
pressure in the receptacle draws approximately 10 milliliters of
blood, and afterwards the double-ended needle is removed first from
the receptacle and then from the donor. It is seen that if the
evacuated container and double-ended needle are sterile, the blood,
schematically represented by numeral 50, will not be contaminated.
It should be noted that the stopper, the double-ended needle, and
the mechanism for evacuating the receptacle, form no part of this
invention and are presented only in the interest of clarity and
understanding of the invention. However, it is contemplated that
these features will be used in the practice of the invention.
After obtaining a specimen of blood as shown in FIG. 4, the stopper
15 is removed and a clotting agent is introduced into the specimen
or the receptacle is allowed to stand, open to the atmosphere, long
enough for coagulation of the blood to occur. Thereafter, the
receptacle undergoes centrifugation until the cells 55 are
compacted in the lower chamber 12, as shown in FIG. 5. Upper
chamber 11 will then contain pure serum. Alternatively, if plasma
is desired rather than serum, the blood may be centrifuged
immediately following the collection of the blood in the
receptacle, or an anticoagulation agent may be added to the
specimen.
Once the cells are compacted through centrifugation in lower
chamber 12 as shown in FIG. 5, plug 20 is inserted into the
constriction. As seen more clearly in FIG. 6, as the plug is urged
through constriction 20 by a force applied through rod 35, the
lower portion 31 of the resilient plug 30 is deformed to comply
with the smaller cross-section of aperture The deformation of lower
portion 31 of the plug 30 also allows the flow of a small amount of
serum from above the compacted cells in lower chamber 12 to pass
into upper chamber 11 to replace the volume occupied by lower
portion 31 of the plug 30. This flow is schematically illustrated
in FIG. 6 as arrows 53. The amount of serum that must flow upward
is determined by the size of lower portion 31 of plug 30. Normally,
the flow is slight and will not cause any disturbing
turbulence.
As lower portion 31 of the plug is deformingly urged through the
constriction, the air bubble in open pocket 34 is slightly
compressed. The slight increase in pressure in lower chamber 12
created by the air bubble in pocket 34 forces the annular shoulder
39 of lower portion 31 of the plug, against the annular wall 29 of
the constriction 20. The result is a barrier producing a positive
seal between the compacted cells 55 in lower chamber 12 and the
serum 56 in upper chamber 11. Once the plug is seated in position,
rod 35 may be twisted and broken off at the weakened area 37. The
handle portion of the rod may then be removed and the serum may
then be decanted, aspirated, etc. for analysis purposes.
Alternatively, stopper 15 may be replaced at the open end of the
receptacle for shipping and/or storage purposes.
FIG. 8 illustrates another embodiment of the plug 30 of the
invention. Plug 30' has the same configuration as plug 30 of the
preferred embodiment; detachable handle 40, however, pulls-away
rather than breaks-away. Conical toe 41 is embedded in the
resilient plug and is simply pulled out once plug 30' is sealingly
positioned in the constriction.
It is readily apparent to those skilled in the art that the
receptacle and plug of the present invention provides a disposable
apparatus for physically separating stratified blood which: can
receive a specimen of blood under sterile conditions; minimizes the
risk of loss of identity of the donor; effectively halts the
migration of cells into the lighter phase once the plug has been
inserted and prevents chemical interaction between the two phases;
and which attains physical separation quickly and easily at a
location in the receptacle determined by the location of the
constriction.
The invention has been described in detail with particular
reference to a preferred embodiment thereof, but it will be
understood that variations and modifications can be effected within
the spirit and scope of the invention.
* * * * *