U.S. patent number 3,661,265 [Application Number 05/058,557] was granted by the patent office on 1972-05-09 for serum separator type container.
This patent grant is currently assigned to Contemporary Research and Development Corporation. Invention is credited to Donald J. Greenspan.
United States Patent |
3,661,265 |
Greenspan |
May 9, 1972 |
SERUM SEPARATOR TYPE CONTAINER
Abstract
Following mechanical or chemical separation of serum or plasma
from the formed elements of blood, the serum or plasma is
physically isolated from the precipitated formed elements by
inserting a plug into the collection tube. The plug consists of a
fibrous filter disc fastened to a rubber member having perforations
which open when the pressure underneath the plug is greater than
the pressure above the plug, but which are otherwise closed. A
handle for manipulating the plug functions as a container to
collect and remove serum or plasma. In an alternative embodiment,
the handle is removably attached to the plug so that the plug can
be left in place in the collection tube, maintaining isolation
between the serum and the formed elements. In another alternative,
the plug is provided with a container for collecting and storing
serum. A similar plug inside the container is used to effect an
additional filtration step.
Inventors: |
Greenspan; Donald J.
(Riverside, NJ) |
Assignee: |
Contemporary Research and
Development Corporation (Philadelphia, PA)
|
Family
ID: |
22017556 |
Appl.
No.: |
05/058,557 |
Filed: |
July 27, 1970 |
Current U.S.
Class: |
210/359; 210/390;
210/398; 210/444; 210/446; 422/44; 422/918 |
Current CPC
Class: |
G01N
33/491 (20130101); B01D 33/01 (20130101); B01L
3/5021 (20130101) |
Current International
Class: |
B01L
3/14 (20060101); B01D 33/01 (20060101); B01D
33/00 (20060101); G01N 33/49 (20060101); B01d
033/00 (); C01b 021/06 () |
Field of
Search: |
;210/497,532,533,390,398,433,429-432,444,448,449,59,354
;23/258.5 |
References Cited
[Referenced By]
U.S. Patent Documents
Primary Examiner: Miga; Frank W.
Claims
I claim:
1. A serum separator suitable to remove serum from a collection
tube of height sufficient to contain a quantity of serum which has
been separated from the formed elements of the blood and wherein
the formed elements of the blood precipitate at the bottom of the
collection tube and the serum positions above the formed blood
elements, the combination of
A. separator means which are insertable into and removable from the
said collection tube,
1. said separator means having a height greater than the height of
the collection tube and terminating in a first end and a second
end,
2. said separator means including means to telescope the separator
means into the collection tube to receive serum and out of the
collection tube to remove serum,
3. said separator means including a serum receiving elongate,
interior space;
B. plug means closing the said first end of the separator
means,
1. a portion of said plug means extending peripherally outwardly
from the separator means a distance sufficient to contact the
collection tube in a sliding, liquid-tight junction,
2. said plug means including one-way fluid valve means,
a. said one way fluid valve means opening into the separator
interior space to permit passage of serum from the collection tube
into the interior space,
b. said one way fluid valve means preventing serum from exiting the
interior space at the said first end; and
C. closure means closing the said second end of the separator
means,
1. said closure means including one way air valve means,
a. said one way air valve means opening in a direction away from
the separator interior space to permit the passage of air from the
interior space when serum enters the interior space,
2.
2. the said separator means, the said plug means and said closure
means cooperating to form a serum shipping container,
a. said shipping container being removable from the collection
tube,
b. said serum shipping container retaining the serum therein after
removal from the collection tube whereby the serum may be
transported to another
location. 2. The invention of claim 1 and filtering means covering
a portion of the plug means, said filtering means being positioned
to filter the serum before it enters the interior space through the
one way fluid valve means.
3. The invention of claim 2 wherein the one way fluid valve means
and the one way air valve means each include a disc of material
resistant to the passage of fluid and air, a hole penetrating a
portion of the disc and terminating within the disc in a resilient
flap, said flap opening in a direction away from the hole to permit
the passage of fluid or air through the disc and closing in a
direction towards the hole to prevent the passage of fluid or air
through the disc in said direction.
4. In a combination serum collection tube and serum shipping
container within which the formed elements of blood precipitate at
the bottom and serum positions above the formed blood elements, the
combination of
A. a hollow cylindrical tube having an open top and a closed
bottom,
1. said tube containing the formed elements of blood and serum
therein,
B. plug means having a bottom and a top,
1. said plug means being insertable into the open top of the tube
and forming a sliding, liquid-tight seal therewith,
2. said plug means including one way fluid valve means opening in a
direction away from the formed elements of the blood,
a. said valve means permitting passage of the serum as the plug
means insert into the tube;
C. an operating handle associated with the plug means and having
separable means to push the plug means into the open top of the
tube,
1. said handle serving to push the plug means into the hollow tube
through the serum toward the formed blood elements,
2. said handle disconnecting from the plug means at the separable
means when the plug means are positioned within the hollow tube;
and
D. a cap closing the open top of the tube after disconnecting the
said handle,
1. said cap sealing the serum within the tube between the plug
means and the cap to permit the tube to be employed as a shipping
container.
5. The invention of claim 4 wherein the plug means are provided
with retainers rising from the top, the retainers including
separable handle engaging means.
6. The invention of claim 4 and filtering means covering a portion
of the plug means, said filtering means being positioned to filter
the serum as it passes through the one way fluid valve means.
7. The invention of claim 6 wherein the one way fluid valve means
include a disc of material resistant to the passage of fluid, said
disc being partially pierced by a hole which penetrates the disc
from the bottom thereof, said hole penetrating a portion of the
disc and terminating upwardly within the disc in a resilient flap,
said flap opening in a direction away from the hole to permit the
passage of fluid through the disc and closing in a direction
towards the hole to prevent the passage of fluid through the disc
from top to bottom.
8. In a serum collection and shipping system for use in conjunction
with a collection tube wherein the formed elements of blood
precipitate at the bottom of the tube and the serum positions above
the formed elements of the blood, the combination of
A. a serum container slidable within the collection tube,
1. said serum container being provided at the bottom thereof with
first plug means and at the top thereof with handle attaching
means,
2. said first plug means including one way fluid valve means which
open in a direction from the bottom to the top,
a. said first plug means admitting serum into the serum container
as the serum container slides downwardly into the collection
tube;
B. second plug means slidable within the serum container,
1. said second plug means having a peripheral ridge in sealing,
sliding engagement with the serum container,
a. said second plug means offering less resistance to sliding
forces than the said first plug means,
2. said second plug means including one way valve means opening in
a direction towards the top of the serum container; and
C. a handle removably connected to the serum container at the
handle attaching means thereof,
1. said handle serving to insert the serum container into the
collection tube,
a. said serum entering the serum container as the serum container
is pushed into the collection tube,
b. said serum sliding the second plug means upwardly within the
serum container,
c. said serum being retained within the serum container by the
first plug means and the second plug means,
2. said handle serving to remove the serum container and serum from
the collection tube,
3. said handle being removed from the serum container after the
serum container has been pulled from the collection tube to permit
the serum container to be employed as a shipping container.
9. The invention of claim 8 wherein the serum container is provided
with fill indication mark, the said peripheral ridge of the second
plug means registering with the fill indication mark when
sufficient serum has passed into the serum container.
10. The invention of claim 1 wherein the portion of the plug means
extending peripherally outwardly from the separator means is
fabricated of resilient material.
11. The invention of claim 8 wherein the first and second plug
means are equipped with filtering means, said filtering means being
positioned to filter the serum as it passes through the one way
valve means.
12. The invention of claim 8 wherein the handle contacts the handle
attaching means near the outer periphery thereof, said handle
applying forces peripherally to the handle attaching means.
13. The invention of claim 1 wherein portion of the plug means
extending peripherally outwardly from the separator is equipped
with reverse valve means, said reverse valve means opening in a
direction opposite to the direction of opening of said one way
valve means.
Description
BACKGROUND OF THE INVENTION
This invention relates to blood analysis, and particularly to an
apparatus and method for maintaining isolation between the formed
elements of blood and the serum or plasma after mechanical or
chemical precipitation of the formed elements has taken place.
Ordinarily, separation of the formed elements (white cells, red
cells and platelets) of blood from the serum is accomplished by the
use of a centrifuge. In my copending application, Ser. No. 847,469,
filed Aug. 4, 1969, there is described a method of chemically
separating serum or plasma from the formed elements of blood by
adding a positively charged polymer and a lectin to the blood in a
collection tube.
With either method, a short time after the formed elements are
precipitated, the red blood cells begin to liberate potassium and
other contaminants which may interfere with the tests performed on
the serum or plasma. Consequently, it is desirable to isolate the
serum or plasma from the formed elements promptly after mechanical
or chemical precipitation. With chemical separation methods, small
amounts of red cells and fibrin may be left in suspension, and it
therefore is also desirable in the case of chemical separation to
remove all of the residual suspended matter from the serum or
plasma.
In the past, attempts have been made to isolate centrifugally
separated serum from the formed elements by the use of a pick-up
device consisting of a first tube fitted at the lower end with a
rubber element adapted to engage and slide along the walls of a
collection tube. The pick-up device has a second tube which passes
through an opening in the rubber element to a point near the upper
end of the first tube, and the second tube has a downwardly facing
opening which delivers serum into the first tube as the pick-up
device is pushed into the collection tube. The device is inserted
into the collection tube, fills with serum, and is then removed
from the collection tube. The serum retained in the device can then
be tested.
A problem with the use of the device just described is that it
cannot be used to transport the separated serum or plasma to the
laboratory for testing from the point at which the blood sample is
taken. Consequently, the collected serum must be transferred to a
suitable container in a time-consuming and error-prone procedure.
This problem becomes serious where blood tests are being performed
for a large number of persons.
If the pick-up device just described were used in conjunction with
a chemical separation method where the presence of suspended debris
in the serum or plasma is likely, the debris would pass into the
first tube of the pick-up device through the second tube.
SUMMARY OF THE INVENTION
In accordance with this invention, the serum separator includes a
plug consisting of a disc having one-way valve openings associated
with a fibrous filter disc which prevents suspended debris and
formed elements from passing through the plug. The plug thus
maintains the separated serum or plasma relatively free of
contaminants.
In accordance with a first embodiment of the invention, there is
provided a separation device which, itself, may be used for
transporting serum or plasma to the laboratory for testing.
In accordance with a second embodiment of the invention, a
separation plug is left in the blood collection tube, and maintains
the formed elements and the serum or plasma isolated from each
other. In this case, the collection tube itself can be used for
transporting the serum or plasma to the laboratory.
In accordance with a third embodiment of the invention, a
separation plug is provided with a container for collecting serum
or plasma. The container has its own internal separation plug
similar to the one to which it is attached. The internal separation
plug is used to effect a second filtration prior to testing of the
serum or plasma.
BRIEF DESCRIPTION OF THE DRAWINGS
FIG. 1 is a vertical section of a blood collection tube showing a
first embodiment of the serum separator as it is being inserted
into the collection tube;
FIG. 2 is a top plan view of the plug at the lower end of the serum
separator shown in FIG. 1;
FIG. 3 is a bottom plan view of the plug at the lower end of the
serum separator shown in FIG. 1;
FIG. 4 is a vertical section taken on the plane indicated at 4--4
in FIG. 2;
FIG. 5 is a vertical section of the closure shown at the upper end
of the serum separator in FIG. 1;
FIG. 6 is a sectional view showing, in detail, a perforation in the
plug of FIG. 4 in the condition which exists when the serum
separator is being inserted into the collection tube;
FIG. 7 is an exploded view of a second embodiment of the invention,
showing a removable handle, a perforated plug, and a fibrous filter
disc in a separated condition;
FIG. 8 is an elevation of a blood collection tube showing the
perforated plug and fibrous filter disc in place maintaining
separation between formed elements in the lower part of the
collection tube and serum or plasma in the upper part of the
collection tube;
FIG. 9 is a vertical section of a blood collection tube showing the
third embodiment of the invention; and
FIG. 10 is a top plan view of the serum container of the third
embodiment of the invention.
DESCRIPTION OF THE PREFERRED EMBODIMENTS
The first embodiment of the invention, by which serum or plasma is
removed from the collection tube in a separate container, is
illustrated in FIGS. 1 through 6.
The collection tube 10 is preferably a tube of a well-known type
used for drawing blood from a patient's vein by means of a vacuum.
A serum separator 12 is shown partially inserted into tube 10.
Separator 12 consists of a tube 14, sufficiently long that, when
the lower end is near the bottom of collection tube 10, its upper
end protrudes beyond the opening at the upper end 16 of the
collection tube.
A plug 18 is fitted into the opening at the lower end of the
separator tube 14, the fit being sufficiently tight that the plug
will not pull out of the separator tube in normal usage. Retaining
means such as inwardly extending ridges (not shown) in the
collection tube may be provided if desired, but are not
necessary.
The details of plug 18 are illustrated in FIGS. 2, 3 and 4. The
plug comprises a disc 20 and an integral upstanding short tube 22,
both preferably consisting of rubber or a synthetic polymer such as
polyvinylchloride. Various other resilient materials may be used so
long as they exhibit sufficient resiliency to provide an adequate
seal between the edge 24 of the disc and the collection tube and to
allow the one-way valves 26 to operate properly.
Valves 26, as shown in FIG. 6, consist of flaps 28 formed in the
rubber and adapted to close off holes 30 which extend part way
through the rubber disc. The valves may be formed in the rubber
disc by first producing holes 30, and then producing V-shaped slits
(FIG. 2) from the top of the disc, which meet the upper ends of the
holes 30.
The upstanding tube 22 fits into the lower end of separator tube
14. Edge 24 is tapered to allow the plug to deform easily as it is
moved into the collection tube.
At the upper end of the separator tube 14, there is provided a
closure 32, also preferably made of rubber or a synthetic polymer.
Closure 32 consists of a disc 34 having an integral tube 36
extending into the upper end of separator tube 14. Closure 32, as
shown in FIG. 5, has one-way valves 38 which are similar to those
in plug 18.
Underneath plug 18, there is fastened a disc 40. Disc 40 preferably
consists of compacted polypropylene fibers, but may also be made up
of fibers of other polyolefins or fibers of other substances which
do not react with blood. The disc 40 is adhered to the underside of
rubber member 20 by a suitable cement, and is arranged with respect
to holes 30 so that all fluid which passes upwardly through holes
30 must have first passed through the filter disc.
In operation of the device just described, blood is first collected
in tube 10 by a conventional collection method. Following
collection, the formed elements are precipitated either
mechanically by the use of a centrifuge, or chemically by the
addition of chemical substances. With a centrifuge, Fibrin is
separated out with the formed elements, leaving serum. With
chemical separation, ordinarily only the formed elements will
separate out, leaving plasma rather than serum. This may be
satisfactory, but if it is desired to obtain serum, a suitable
clotting agent such as thrombin may be used to precipitate out
Fibrin so that serum rather than plasma is left over.
After the formed elements are precipitated so that they collect at
the bottom of the collection tube 10 at 42, the separator 12 is
inserted into the collection tube as shown in FIG. 1. The serum or
plasma 44 passes through filter disc 40, and through the one-way
valves 26 into the interior of tube 14. The air which is displaced
passes through one-way valves 38 in closure 32. The separator is
moved downwardly to a point just above the interface between the
formed elements and the serum or plasma, and is then withdrawn from
the collection tube. As the separator is withdrawn, air is drawn
between edge 24 of the plug and the wall of the collection tube
into the lower part of the collection tube. The serum or plasma
within tube 14 cannot pass through valves 26 in the opposite
direction, and consequently remains within the separation tube. The
separation tube itself may then be marked for identification and
sent to the laboratory for testing. The separator 12 is suitable
for shipping in styrofoam containers.
An alternative device is shown in FIG. 7, which is an exploded
view. A plug comprises a resilient element 46 and a fibrous filter
disc 48. Element 46 and disc 48 are normally cemented together.
Element 46 is made of resilient rubber or rubber-like materials
such as polyvinyl-chloride, and disc 48 is made from olefin fibers
or other fibers such as nylon, which do not react with blood. Disc
46 has one-way valves 50 which are identical to the one-way valves
shown in FIG. 6. These valves are so arranged in element 46 that
any blood which passes through them must first have passed through
disc 48.
Retainers 52 are attached to the top surface 54 of element 46. The
retainers have overhanging parts 56 which are adapted to receive
tabs 58 attached at the lower end of a tubular plastic or paper
handle 60. Handle 60 is attached to the plug consisting of element
46 and disc 48 by twisting the tube 60 in a clockwise direction
(looking downwardly) so that tabs 58 are held between overhanging
parts 56 of retainers 52 and the surface 54. The entire assembly in
FIG. 7 can then be inserted into a collection tube 62 (FIG. 8) in
which formed elements have been precipitated out of serum or
plasma, and the tube 62 can be detached from the plug by
counterclockwise rotation when the plug is in place as shown in
FIG. 8. The collection tube 62 can then be capped and sent to the
laboratory for testing of the serum or plasma 64. The serum or
plasma remains isolated from the formed elements 66 at the bottom
of the tube by the plug; the one-way valves 50 remain closed, and
the potassium which is liberated by the red cells cannot
contaminate the serum or plasma 64.
FIG. 9 shows a collection tube 66, in which there is located a
separation plug 68 consisting of a rubber disc 70 attached to a
fibrous filter disc 72. Disc 70 has one-way valves 74 similar to
those previously described. It is also provided with an integral
upstanding short tube 76 having an outwardly extending lip 78
attached to engage the inwardly extending lip 80 of container 82 to
provide a secure and liquid-tight seal with inwardly extending lip
80 fitting tightly into the groove 84 between lip 78 and the upper
part of disc 70. Container 82 has a cylindrical inner wall 86, and
a top closure 88 having a relatively narrow central opening 90. Top
closure 88, as more clearly shown in FIG. 10, has retainers 92
similar to those indicated at 52 in FIG. 7. These retainers are
adapted to engage tabs 94 on a handle 96 which is similar to handle
60 in FIG. 7. Handle 96 is detachable from container 82 by virtue
of the removable engagement of tabs 94 underneath the overhanging
parts of retainers 92.
Within container 82 there is provided a second plug 98 consisting
of a rubber element 100 attached to a fibrous filter disc 102
similar to disc 72. Element 100 has a pair of ridges 101 and 103
which are in sealing engagement with wall 86, but which allow
relatively easy axial sliding of plug 98 within container 82, so
that the pressure required to cause the plug to slide is less than
that required to open the one-way valves. Disc 100 is also provided
with openings in the form of one-way valves, which permit flow of
liquid upwardly through the plug as it is moved downwardly within
container 82. One such opening is shown at 105. These one-way
valves, like the one-way valves in the other embodiments are
arranged so that any liquid which flows through the one-way valves
must first have passed through the fibrous filter disc.
A mark 104 is provided on the outer surface of container 82 for the
purpose of indicating that a particular volume of serum or plasma
has been collected when a predetermined one of the ridges, for
example ridge 101, comes into register with the mark.
In operation, the container 82 is first attached to handle 96, and
plug 98 is in the lowermost possible position within container 82.
The assembly is pushed downwardly into the collection tube 66, as
shown in FIG. 9. As the plasma or serum 106 passes through one-way
valve openings 74, it begins to fill space 108 underneath plug 98
and within container 82. Plug 98 moves upwardly with respect to
container 82 as the container is pushed downwardly into collection
tube 66. When ridge 101 comes in to register with mark 104, the
assembly is pulled out of the collection tube, handle 96 is
removed, and the container 82, now containing serum, can be packed
in a styrofoam container, for example, and shipped to the
laboratory for testing. At the laboratory, plasma or serum 108 in
container 82 can be subjected to a second filtration to remove any
residual debris by pushing plug 98 downwardly with a suitable
implement inserted through opening 90. The serum or plasma, now
above plug 98, can be poured through opening 90 into any suitable
container for testing.
Various modifications can be made to the three embodiments
disclosed. The valves in the plugs, for example, can take various
forms and need not consist of holes communicating with V-shaped
slits. Any similar valve which opens under the influence of a
differential pressure may be used. Valves which permit flow in one
direction, but which prohibit flow in the other direction are not
absolutely necessary for the operation of the invention. In the
embodiment shown in FIG. 1, for example, even if the valves in plug
18 were not one-way valves, the one-way valves in closure 32 would
prevent serum or plasma from escaping from the separator tube 14
when it is withdrawn. A closure having no valves at all could be
substituted for the closure 32, but it would have to be removed
when the separator is inserted into the collection tube. Various
other modifications to the invention can be made. The valves may be
used in any number or placed in any position along the separator to
assure its proper functioning. One or more check valves can be
located at edge 24 of plug 18 to further facilitate the flow of air
into the lower part of the collection tube when the serum separator
12 is withdrawn. Valves 25 inverted from the orientation shown in
FIG. 4 are suitable.
* * * * *