U.S. patent number 3,873,217 [Application Number 05/382,240] was granted by the patent office on 1975-03-25 for simplified rotor for fast analyzer of rotary cuvette type.
This patent grant is currently assigned to The United States of America as represented by the United States Atomic. Invention is credited to Norman G. Anderson, Damous D. Willis.
United States Patent |
3,873,217 |
Anderson , et al. |
March 25, 1975 |
Simplified rotor for fast analyzer of rotary cuvette type
Abstract
A simplified rotor design utilizing two or less cavities per
sample analysis station is described. Sample or reagent liquids are
statically loaded directly into respective sample analysis cuvettes
by means of respective apertures and centripetal ramps
communicating with each cuvette. According to one embodiment, a
single static loading cavity communicates with each sample analysis
cuvette in a conventional manner to facilitate dynamic transfer of
liquid from that cavity to the cuvette where mixing of sample and
reagent liquids and their photometric analysis take place. Dynamic
loading of sample or reagent liquids is provided in another
embodiment.
Inventors: |
Anderson; Norman G. (Oak Ridge,
TN), Willis; Damous D. (Clinton, TN) |
Assignee: |
The United States of America as
represented by the United States Atomic (Washington,
DC)
|
Family
ID: |
23508095 |
Appl.
No.: |
05/382,240 |
Filed: |
July 24, 1973 |
Current U.S.
Class: |
356/246; 422/547;
356/427; 250/576; 422/72 |
Current CPC
Class: |
B01F
5/0068 (20130101); B01F 15/0233 (20130101); G01N
21/07 (20130101); B01F 13/0059 (20130101) |
Current International
Class: |
G01N
21/03 (20060101); B01F 5/00 (20060101); G01N
21/07 (20060101); B01F 13/00 (20060101); G01n
001/10 () |
Field of
Search: |
;23/253R,259
;356/36,197,244,246 ;250/573,576 |
References Cited
[Referenced By]
U.S. Patent Documents
Primary Examiner: Wibert; Ronald L.
Assistant Examiner: Evans; F. L.
Attorney, Agent or Firm: Horan; John A. Zachry; David S.
Hamel; Stephen D.
Government Interests
BACKGROUND OF THE INVENTION
The invention described herein relates generally to photometers and
more particularly to an improved rotor for fast analyzers of the
rotary cuvette type characterized by two or less cavities per
sample analysis station. It was made in the course of, or under, a
contract with the U.S. Atomic Energy Commission.
Claims
What is claimed is:
1. An improved rotor for use in a fast photometric analyzer of the
rotary cuvette type comprising a disk-shaped member of laminated
construction with a central opaque disk sandwiched between top and
bottom transparent walls, said disk-shaped member defining:
a, circular array of sample analysis cuvettes extending through
said central opaque disk for receiving and holding samples and
reagents for photometric analysis;
b. a circular array of outwardly and downwardly extending ramps
defining cuvette loading passageways, each of said ramps being in
communication with the top end of the centripetal side of a
respective cuvette in said array of sample analysis cuvettes;
c. a circular array of first loading apertures extending through
said top transparent wall in axial register and liquid
communication with respective ramps in said circular array of ramps
for facilitating the static loading of liquid in said cuvettes;
and
d. means for dynamically injecting liquids into said sample
analysis cuvettes.
2. The improved rotor of claim 1 wherein said means for dynamically
injecting liquids into said sample analysis cuvettes comprises an
array of static loading cavities equal in number and disposed
centripetal to said sample analysis cuvettes, second loading
apertures extending through said top transparent wall in register
with each of said static loading cavities and connecting
passageways extending between respective cavities in said array of
static loading cavities and said sample analysis cuvettes.
3. The improved rotor of claim 2 wherein said disk-shaped member
further defines a central distribution chamber and a plurality of
distribution passageways communicating between said distribution
chamber and respective cuvettes in said circular array of sample
analysis cuvettes.
4. The improvement of claim 3 wherein each of said distribution
passageways intersects with adjacent distribution passageways to
form a serrated periphery about said distribution chamber.
5. The improvement of claim 3 wherein each of said distribution
passageways has a capillary-sized portion.
6. The improved rotor of claim 1 wherein said means for dynamically
injecting liquids into said sample analysis cuvettes comprises a
central distribution chamber and a plurality of distribution
passageways communicating between said distribution chamber and
respective cuvettes in said circular array of sample analysis
cuvettes.
7. The improved rotor of claim 6 wherein each of said distribution
passageways intersects with adjacent distribution passageways to
form a serrated periphery about said distribution chamber.
8. The improvement of claim 6 wherein each of said distribution
passageways has a capillary-sized portion.
Description
The general design and operation of fast analyzers of the rotary
cuvette type are generally described in U.S. Pat. No. 3,555,284,
issued Jan. 12, 1971, to common assignee in the name of Norman G.
Anderson. In the analyzer described in that patent, a central
loading disk is provided for statically receiving sample and
reagent liquids prior to an analysis operation. An annular array of
sample analysis cuvettes is disposed about the central loading disk
for receiving the liquids from the loading disk and holding them
for photometric analysis. A series of four serially interconnected
cavities are required per sampling station: two static loading
cavities for receiving sample and reagent liquids, one mixing
chamber, and one sample analysis cuvette. Because of space
limitations, more recently developed miniaturized fast analyzer
designs do not incorporate separate mixing chambers and require
only three cavities per sampling station. Typical three-cavity
rotor designs are shown in U.S. Pat. No. 3,798,459 issued Mar. 19,
1974, in the name of Anderson, et al., and U.S. Pat. No. 3,795,451,
issued Mar. 5, 1974, in the name of Mailen.
The radially innermost static loading cavities which are part of
each sampling station limit the total number of sampling stations
because of the lack of available rotor space at the smaller radius.
In the more recently developed miniaturized fast analyzers
identified above, rotors with diameters of 3.5 inches or less are
severely limited where three-cavity sampling stations are used.
It is, accordingly, a general object of the invention to provide an
improved rotor for a fast photometric analyzer of the rotary
cuvette type characterized by a minimum number of cavities per
sampling station.
Another, more particular object of the invention is to provide an
improved rotor for a fast photometric analyzer of the rotary
cuvette type having two or less cavities per sampling station.
SUMMARY OF THE INVENTION
In a fast photometric analyzer of the rotary cuvette type, a
simplified rotor design is provided requiring two or less cavities
per sampling station. Sample or reagent liquid is loaded directly,
by means of a centripetal ramp, into each sample analysis cuvette
under static conditions. A single additional static loading cavity
is disposed centripetal to each sample analysis cuvette according
to one embodiment. Dynamic transfer of liquid from the static
loading cavities to respective cuvettes is effected dynamically by
conventional means such as radially extending passageways adapted
to discharge tangentially into the cuvettes. According to another
embodiment, static loading of each cuvette is followed by dynamic
loading of a single sample or reagent liquid. Such arrangement
permits the greatest number of sample analysis stations for any
given size rotor and is especially suitable in miniaturized rotors
where space restrictions are greatest.
BRIEF DESCRIPTION OF THE DRAWINGS
FIG. 1 is a top plan view of a rotor made in accordance with the
invention.
FIG. 2 is a vertical section view of the rotor of FIG. 1.
FIG. 3 is a top plan view of an alternative rotor design suitable
for completely static or static and dynamic loading of sample and
reagent liquids.
FIG. 4 is a vertical section view of the rotor of FIG. 3.
FIG. 5 is a bottom plan view of the rotor of FIGS. 3 and 4.
FIG. 6 is a top plan view of an alternative rotor design suitable
for combined static and dynamic loading.
FIG. 7 is a vertical section view of the rotor of FIG. 6.
FIG. 8 is a bottom plan view of the rotor of FIGS. 6 and 7.
DESCRIPTION OF THE PREFERRED EMBODIMENTS
Referring to the drawings, initially to FIGS. 1 and 2, each
sampling station 1 (only two of thirty-two shown) is seen to
comprise a sample analysis cuvette 2 and a single static loading
cavity 3. The rotor 4 is a laminar construction with an opaque
mid-section 5 sandwiched between top and bottom transparent plates
6 and 7. As shown, sample analysis cuvettes 2 and static loading
cavities 3 are conveniently formed by means of holes or slots in
opaque mid-section 5 with plates 6 and 7 providing end closures.
Static loading is accomplished by respectively injecting, using a
syringe or other suitable means, sample and reagent liquids into
the sample analysis cuvettes 2 and cavities 3 under static
conditions. Apertures 8 and 9 are provided in top plate 6 to
facilitate such loading. A ramp 11 is provided on the centripetal
side of each cuvette 2 to permit direct static loading of liquid
into the cuvette without incurring spillage during rotation of the
rotor. Any liquid retained on the ramp following static loading of
cuvette 2 is dynamically transferred to the cuvette upon rotation
of the rotor.
Dynamic transfer of liquid from each static loading cavity to a
corresponding cuvette 2 occurs through passageway 12 which opens at
the top centrifugal side of cavity 3. As shown in FIG. 1,
passageway 12 discharges tangentially into cuvette 2 to enhance
mixing therein of sample and reagent liquids. A slight outward
inclination of each cavity 3 aids in the rapid dynamic transfer of
liquids from those cavities. Other conventional means for
permitting dynamic transfer of liquid from cavities 3 to cuvettes 2
could be used without departing from the invention such as the
capillary passageway and bubble trap described in copending
application Ser. No. 354,041 now U.S. Pat. No. 3,795,451, of common
assignee.
FIGS. 3, 4, and 5 show an alternative embodiment of the invention
in top, vertical section, and bottom views, respectively. Like,
though primed, reference numerals are used to designate like
features of the alternative rotor embodiment. As in the embodiment
of FIGS. 1 and 2, an annular array of sample analysis cuvettes 2'
and static loading cavities 3' is provided with passageways 12'
joining corresponding cuvettes and cavities. Loading apertures 8'
and 9' discharge directly into cuvette 2' and static loading cavity
3' . A dynamic liquid system is also included which may be used
where it is desired to perform a multiplicity of tests on a single
sample or a single test on a multiplicity of samples, thereby
providing a great degree of flexibility to the rotor and making it
amenable to virtually any testing situation. Operation of the rotor
using static loading only is possible in the manner described above
in reference to the embodiment of FIGS. 1 and 2.
The dynamic distribution system includes a central distribution
chamber 15 provided with a serrated periphery 16 which causes
liquid fed therein while the rotor is spinning to be substantially
equally distributed to the cuvettes 2'. Radial passageways 17,
which have capillary sized portions 18 to prevent flow from the
cuvettes under static conditions, extend between distribution
chamber 15 and each cuvette. In operation, either sample or reagent
liquids could be statically loaded in the cuvettes, the rotor spun,
and respective reagent or sample liquids dynamically injected to
mix with the statically loaded liquids. As shown, static loading
chamber 3' does not extend completely through opaque mid-section 5
in order that space be available for radial passageways 17.
Another embodiment, limited in use to dynamic loading of sample or
reagent liquids, is illustrated in FIGS. 6, 7, and 8. As shown in
those figures, an array of sample analysis cuvettes 2" is provided
in a manner similar to that of the embodiment of FIGS. 3, 4, and 5.
No separate static loading cavities are provided, however, since
only one liquid is statically loaded into the cuvettes. A dynamic
loading system as described with reference to the embodiment of
FIGS. 3, 4, and 5 is provided with like, though double primed,
reference numerals designating like features. In operation, sample
or reagent is statically loaded in the cuvettes followed by dynamic
loading of respective reagent or sample liquid through the dynamic
loading system. Rotors made in this manner are limited in number of
sample analysis stations only by the number of cuvettes which can
be spaced about their peripheries.
The foregoing description of three embodiments of the invention is
offered for illustrative purposes only and should not be
interpreted in a strictly limiting sense. For example, connecting
passageway 12 extending from the top of each static loading cavity
3, 3' may be replaced by a capillary passageway and bubble trap in
the manner described in U.S. Pat. No. 3,795,451. It is intended,
rather, that the invention be limited only be the scope of the
claims attached hereto.
* * * * *