U.S. patent number 3,795,451 [Application Number 05/354,041] was granted by the patent office on 1974-03-05 for rotor for fast analyzer of rotary cuvette type.
This patent grant is currently assigned to The United States of America as represented by the United States Atomic. Invention is credited to James C. Mailen.
United States Patent |
3,795,451 |
Mailen |
March 5, 1974 |
ROTOR FOR FAST ANALYZER OF ROTARY CUVETTE TYPE
Abstract
An improved rotor characterized by efficient premixing of sample
and reactant liquids prior to their being discharged into a sample
analysis cuvette is provided. Inner and outer concentric circular
arrays of static loading cavities are disposed within the rotor on
a one-to-one basis centripetal to an array of sample analysis
cuvettes. The centrifugal walls of cavities in the inner array of
loading cavities each slope downwardly and outwardly to a
capillary-sized passage having a bubble trap. Liquid communication
is provided by the capillary-sized passages between those cavities
and respective cavities in the outer array of loading cavities upon
rotation of the rotor while intercontact of the liquids in the
respective cavities is prevented under static loading conditions.
The centrifugal walls of cavities in the outer array of loading
cavities each slope upwardly and outwardly to a cuvette loading
passageway at an angle approaching the vertical in order to retain
liquids in those cavities at preselected low rotor rotational
speeds sufficient to cause liquid to flow through the
capillary-sized passage and to discharge liquids through the
cuvette loading passageways at preselected higher speeds.
Inventors: |
Mailen; James C. (Oak Ridge,
TN) |
Assignee: |
The United States of America as
represented by the United States Atomic (Washington,
DC)
|
Family
ID: |
23391648 |
Appl.
No.: |
05/354,041 |
Filed: |
April 24, 1973 |
Current U.S.
Class: |
356/246; 250/576;
422/72 |
Current CPC
Class: |
B01F
15/0233 (20130101); B04B 5/0407 (20130101); B01F
5/0068 (20130101); G01N 21/07 (20130101); B01F
13/0059 (20130101) |
Current International
Class: |
B04B
5/04 (20060101); B04B 5/00 (20060101); B01F
5/00 (20060101); G01N 21/07 (20060101); G01N
21/03 (20060101); B01F 13/00 (20060101); G01n
001/10 (); G01n 001/28 () |
Field of
Search: |
;23/253R,259 ;250/218
;356/36,180,185,197,244,246 |
References Cited
[Referenced By]
U.S. Patent Documents
Primary Examiner: Wibert; Ronald L.
Assistant Examiner: Evans; F. L.
Attorney, Agent or Firm: Horan; John A. Zachry; David S.
Hamel; Stephen D.
Claims
1. An improved rotor for a fast analyzer of the rotary cuvette type
characterized by efficient premixing of sample and reactant liquids
prior to their being discharged into sample analysis cuvettes for
photometric analysis, the improved rotor comprising a generally
disk-shaped member defining:
a. a circular array of sample analysis cuvettes for accepting
mixtures of liquid samples and reactants, said disk-shaped member
having transparent walls above and below said sample analysis
cuvettes;
b. an inner array of static loading cavities equal in number and
disposed centripetal to said sample analysis cuvettes, the
centrifugal walls of said cavities in said inner array of cavities
extending downwardly and outwardly;
c. an outer array of static loading cavities equal in number and
disposed radially intermediate said sample analysis cuvettes and
said cavities in said inner array of static loading cavities, the
centrifugal walls of said cavities in said outer array of cavities
extending upwardly and outwardly with a steep inclination selected
to retain liquids in said cavities at preselected low rotor
rotational speeds;
d. capillary-sized passages extending between respective cavities
in said inner and outer arrays of cavities, said passages being
sized to prevent flow between said cavities under static conditions
while permitting flow from cavities in said inner array of cavities
to cavities in said outer array of cavities at preselected low
rotational speeds; and
e. connecting passages extending between respective cavities in
said outer
2. The improved rotor of claim 1 further including a bubble trap
disposed
3. The improved rotor of claim 1 wherein cavities in said outer
array of loading cavities are generally round in horizontal cross
section and wherein said capillary-sized passages intersect said
cavities in said
4. The improved rotor of claim 3 wherein said sample analysis
cuvettes are generally round in horizontal cross section and
wherein said connecting
5. The improved rotor of claim 1 wherein said capillary-sized
passages extend between the lower ends of respective cavities in
said inner and
6. The improved rotor of claim 5 wherein said capillary-sized
passages intersect with the centrifugal walls of said cavities in
said inner array
7. The improved rotor of claim 1 wherein said connecting passages
extend between the upper ends of respective cavities in said outer
array of
8. The improved rotor of claim 7 wherein said connecting passages
intersect with the centrifugal walls of said cavities in said outer
array of cavities.
Description
BACKGROUND OF THE INVENTION
The invention described herein relates generally to photometers and
more particularly to an improved rotor characterized by efficient
premixing of sample and reagent liquids for use in a photometric
analyzer of the rotary cuvette type. It was made in the course of,
or under, a contract with the U. S. Atomic Energy Commission.
The design and operation of fast analyzers of the rotary cuvette
type are generally described in U. S. Pat. No. 3,555,284, issued
Jan. 12, 1971, to common assignee in the name of Norman G.
Anderson. In the analyzer described in that patent, a central
loading disk is provided for receiving liquid samples and reactants
prior to an analysis operation. An annular array of sample analysis
cuvettes is disposed about the loading disk for receiving the
liquids from the loading disk and holding them for photometric
analysis. Rotation of the loading disk and cuvette array causes the
liquid samples and reactants to flow from the loading disk to
cuvettes where they are photometrically analyzed while the entire
system of cuvettes and loading disk is rotating.
The loading disk described in the aforementioned patent consists of
three concentric annular arrays of chambers, the chambers in each
array being interconnected, in series, with corresponding chambers
in adjacent arrays. Rotation of the loading disk causes sample and
reagent liquids in the two innermost arrays of chambers (loading
chambers) to flow into the outermost array of chambers (mixing
chambers) and then outward through a discharge passage into
corresponding cuvettes. Such arrangement, although generally
satisfactory, is characterized by relatively low mixing
efficiencies since liquids in the innermost array of loading
chambers must overtake the liquid from the radially intermediate
array of chambers before mixing can occur.
In more recently developed fast analyzers of the rotary cuvette
type, miniaturized rotors are used wherein the loading cavities and
sample analysis cuvettes are provided in a single integral rotor.
Such rotors are described in copending applications of common
assignee Ser. Nos. 295,980 and 316,628. Due to the limited
aVailable space in the miniaturized rotors, separate mixing
chambers cannot be used without a substantial reduction in the
number of samples which can be accommodated in a single rotor. The
small cavities and passages used in the miniaturized rotors also
reduce turbulent mixing of the liquid samples and reagents because
of the relatively greater effect of surface tension in the
miniaturized rotors.
It is, accordingly, a general object of the invention to provide an
improved rotor for a fast analyzer of the rotary cuvette type
characterized by efficient premixing of statically loaded sample
and reactant liquids.
Another, more particular, object of the invention is to provide an
improved miniaturized multi-sample rotor for fast analyzers of the
rotary cuvette type characterized by efficient premixing of
statically loaded sample and reactant liquids.
Other objects of the invention will be apparent from an examination
of the following description of a preferred embodiment and the
appended claims.
SUMMARY OF THE INVENTION
In accordance with the invention, an improved rotor is provided for
use in fast analyzers of the rotary cuvette type. The rotor defines
inner and outer circular arrays of static loading cavities or
chambers disposed on a one-to-one basis centripetal to a circular
array of sample analysis cuvettes. The centrifugal walls of
cavities in the inner circular array of loading cavities each slope
downwardly and outwardly to a respective capillary-sized passage
having a bubble trap to prevent liquid flow therethrough under
static conditions. Liquid communication between cavities in the
inner and outer arrays of cavities is provided by the
capillary-sized passages upon rotation of the rotor at a first
preselected low speed. Tangential discharge of flow by the
capillary-sized passages at a point adjacent the lower ends of the
cavities in the outer array causes vortex flow therein and
facilitates mixing. The centrifugal walls of cavities in the outer
array of loading cavities each slope outwardly and upwardly to a
respective cuvette loading passageway at an angle approaching the
vertical in order to retain liquids in those cavities at selected
low rotational speeds sufficient to cause liquid flow through the
capillary-sized passage and to discharge liquids through the
cuvette loading passageways at preselected higher speeds. Such
arrangement permits, by selective rotor acceleration, selective
transfer of liquid from cavities in the inner array of loading
cavities to respective cavities in the outer array and mixing with
liquid retained in those cavities prior to transfer to respective
sample analysis cuvettes.
BRIEF DESCRIPTION OF THE DRAWINGS
FIG. 1 is a top plan view of a cuvette rotor made in accordance
with the invention.
FIG. 2 is a vertical section view of the rotor of FIG. 1.
DESCRIPTION OF PREFERRED EMBODIMENT
Referring now to the drawings, initially to FIG. 1, a rotor 1 made
in accordance with the invention is of laminated construction with
a central, preferably opaque, plastic disk 2 sandwiched between top
and bottom transparent plastic disks 3 and 4. The rotor is drawn
enlarged to clarify construction details.
As further illustrated in FIG. 2, a multiplicity (only three shown)
of sample analysis chambers or cuvettes 5 are disposed in a
circular array within rotor 1 at an equal distance from the center
of that rotor. Cuvettes 5 are conveniently formed by drilling
axially extending apertures through disk 2 prior to sandwiching
that disk between disks 3 and 4. Concentric annular arrays of
sample and reactant loading cavities 6 and 7 are disposed on a
one-to-one basis in general radial alignment with each cuvette 5.
As shown in FIG. 1, loading cavities 6 and 7 are formed as
apertures in disk 2 and are closed by top and bottom disks 3 and 4.
Sample loading cavities 6 are extended below disk 2 by means of a
depression 8 machined into bottom disk 3. Loading apertures 9 and
10 are provided in top transparent disk 3 in register with each
cavity in the respective arrays of sample and reactant loading
cavities. Static loading of reactants and samples through the
loading apertures is possible using a hyperdermic syringe or
automated dispensing equipment.
Successful operation of the invention depends upon the particular
inclinations of the centrifugal surfaces used in the loading
cavities 6 and 7 and the placement of connecting passages 12 and 13
used to connect respective reactant and sample loading cavities and
sample analysis cuvettes.
As shown in FIG. 1, the centrifugal surface 14 of reactant loading
cavity 7 extends downwardly and outwardly to a capillary-sized
passage 12 containing a bubble trap 15. As used herein, a
capillary-sized passage is one sized sufficiently small so as to
block passage of liquid having a head equal to that of a full
adjacent cavity under static conditions. Thus, passage 12 prevents
contact between sample and reactant liquids placed in loading
cavities 6 and 7 while the rotor is at rest as during a loading
operation. Bubble trap 15 supplements the blocking action of
passage 12 under static conditions. At a predetermined low
rotational speed, rotation induced pressure in the reactant liquid
overcomes the flow resistance of passage 12 and bubble trap 15 and
causes reactant to flow from cavity 7 into the bottom of sample
loading cavity 6 where it mixes with sample liquid loaded therein.
As shown in FIG. 2, passage 12 intersects sample loading cavity 6
tangentially to facilitate vortex mixing of reactant and sample
liquids. Sample loading cavity 6 is provided with a circular cross
section so as not to interfere with the vortex flow pattern
illustrated by the broken arrow 16 in FIG. 2. Depression 8 at the
bottom of each sample loading cavity also enhances mixing by
extending the sample loading cavity slightly below the discharge
point of passage 12, thereby ensuring that reactant discharging
from that passage will impinge upon the main body of sample liquid.
The inclination of surface 14 aids in the rapid and complete
transfer of reactant from cavity 7 through passage 12.
Centrifugal surface 17 of sample loading cavity 6 slopes upwardly
and outwardly at a very slight angle (about 5.degree. to
10.degree.) from the vertical. This steep inclination acts to
retain the sample and reactant liquids in cavity 6 until a
predetermined higher rotational speed is reached wherein rotation
induced acceleration forces overcome the gravitational, adhesive,
and surface tension forces tending to retain liquid in the bottom
of cavity 6 and cause it to flow upward and outward through passage
13 joining sample loading cavity 6 with a sample analysis cuvette
5. As shown in FIG. 2, passage 13 discharges into cuvette 5
tangentially to cause further mixing of the sample and reactant
liquids in the cuvette.
In operation, sample and reactant liquids are introduced into
respective loading chambers 7 and 6 with rotor 1 at rest. The
combination of possible reactants and samples is limited in number
only by the number of sample analysis cuvettes 5 available in a
particular rotor. The liquids remain out of contact while the rotor
is at rest as a result of the air lock in capillary-sized passage
12 and/or bubble trap 15. At a predetermined intermediate rotor
speed, reactant flows from each chamber 6 through passage 12 and
into a respective chamber 7 where it mixes with sample liquid.
After a suitable mixing interval, the rotor speed is increased
until the liquid in each chamber 7 passes through passage 13 to a
respective sample analysis cuvette.
The above description of one embodiment of the invention should not
be interpreted in a limiting sense. For example, sample and
reactant liquids could be loaded exactly opposite to the manner
described with sample being placed in cavity or chamber 6 and
reactant in chamber 7. It is intended rather, that the invention be
limited only by the scope of the appended claims.
* * * * *