U.S. patent number 3,814,258 [Application Number 05/341,457] was granted by the patent office on 1974-06-04 for blood plasma separator with filter.
This patent grant is currently assigned to Dickinson and Company. Invention is credited to Waldemar A. Ayres.
United States Patent |
3,814,258 |
Ayres |
June 4, 1974 |
BLOOD PLASMA SEPARATOR WITH FILTER
Abstract
A piston having spaced resilient annular seals and filter
material disposed about the piston between the spaced seals is
adapted to be inserted into an open end of a collection tube so
that the resilient seals engage the interior surface of the tube
and form a seal on each side of the filter material which also
contacts both the piston and the interior of the tube. The
collection tube is adapted to receive blood for separation into its
light and heavy phases. A pusher is detachably connected to the
piston for allowing the piston to be pushed in a downward direction
through the light phase of the blood so that a pressure is created
below the piston which causes the resilient seals to temporarily
deform and leak, thereby allowing the light phase to flow in an
upward direction around the piston and through the filter material
into a space above said piston. When the piston reaches an
interface between the light and heavy phases the downward movement
is terminated and the push rod is withdrawn after being detached
from the piston. The piston remains in place as a permanent barrier
between the light and heavy phases of the blood contained in the
collection tube. A stopper is provided to be inserted into the open
end of the collection tube so that the separated phases of the
fluid may be shipped or stored in the collection tube for future
laboratory tests.
Inventors: |
Ayres; Waldemar A. (Rutherford,
NJ) |
Assignee: |
Dickinson and Company
(Rutherford, NJ)
|
Family
ID: |
23337663 |
Appl.
No.: |
05/341,457 |
Filed: |
March 15, 1973 |
Current U.S.
Class: |
210/359; 210/446;
422/918; 210/390; 210/444 |
Current CPC
Class: |
B01D
33/01 (20130101); B01L 3/5021 (20130101) |
Current International
Class: |
B01L
3/14 (20060101); B01D 33/01 (20060101); B01D
33/00 (20060101); B01d 033/00 () |
Field of
Search: |
;210/DIG.23,359,390,398,444,446 ;23/258.5 |
References Cited
[Referenced By]
U.S. Patent Documents
Primary Examiner: Hart; Charles N.
Assistant Examiner: Calvetti; F.
Attorney, Agent or Firm: Kane, Dalsimer, Kane, Sullivan and
Kurucz
Claims
What is claimed is:
1. An apparatus for effecting a seal between separated light and
heavy phases of blood and for filtering the separated light phase,
comprising:
a collection tube having an open end adapted to receive a sample of
blood for separation into light and heavy phases wherein the heavy
phase precipitates at the bottom of the collection tube and the
light phase accumulates above the heavy phase;
a member adapted for insertion into the open end of the collection
tube;
resilient seal means associated with said member for engaging an
interior surface of said collection tube and for forming a seal
between the member and the tube;
means for inserting said member into the open end of the collection
tube and for exerting a force on the member to urge the member into
the collection tube, whereby a pressure is developed in the tube,
said resilient seal means being responsive to the pressure to
deform and leak; and
filter means disposed adjacent said seal means, said filter means
being formed and arranged to filter any fluid leaking past the seal
means so that when the member is urged into the collection tube
containing a separated blood sample the seal means leaks allowing
the light phase to flow past the seal and through the filter and
upon termination of the force a seal is effected between the
filtered light phase and the remainder of the sample.
2. An apparatus as described in claim 1, wherein the resilient seal
means is disposed about said member for engaging the interior
surface of said collection tube and for forming an annular
seal.
3. An apparatus as described in claim 1, wherein the member is in
the form of a piston and the resilient seal means comprises a pair
of spaced annular seals disposed about said member and the filter
means is disposed between the spaced annular seals.
4. An apparatus as described in claim 3, wherein the piston and
seals are resilient.
5. An apparatus as described in claim 1, wherein the resilient seal
means is formed integrally with said member.
6. An apparatus as described in claim 3, wherein the pair of spaced
annular seals are formed integrally with the member.
7. An apparatus as described in claim 6, wherein the annular seals
are formed by outwardly extending rings.
8. An apparatus as described in claim 7, wherein the outwardly
extending rings are disposed in a conical surface having a vertex
positioned towards the bottom end of the tube.
9. An apparatus as described in claim 1, wherein the filter means
is in the form of a sleeve disposed about said member.
10. An apparatus as described in claim 1, wherein the means for
inserting said member into the open end of the collection tube
comprises a pusher.
11. An apparatus as described in claim 10, wherein the pusher is
detachably connected to the member.
12. An apparatus as described in claim 11, wherein the member is
resilient and the pusher includes a needle extending from a lower
end thereof and inserted into the member for detachable connection
with said member.
13. An apparatus as described in claim 1, additionally comprising a
stopper for closing the open end of the tube after effecting the
sealed separation of the blood phases so that the separated blood
may be stored or shipped to a laboratory for testing.
14. A separator apparatus for use with a blood collection tube to
form a permanent barrier between separated light and heavy phases
of blood contained in the collection tube, comprising:
a piston adapted for insertion into the collection tube;
resilient seal means disposed about said piston for engaging an
interior surface of said collection tube and forming an annular
seal between said piston and said interior surface, said annular
seal being responsive to a predetermined pressure to temporarily
deform and leak;
filter means disposed about said piston, and filter means formed
and arranged to engage both said piston and the interior surface of
said collection tube when said piston is inserted into said
collection tube; and
means for inserting said piston into an open end of a collection
tube and for exerting a force on said piston urging said piston
into said collection tube.
15. An apparatus for effecting a seal between separated light and
heavy phases of blood and for filtering the separated light phase,
comprising:
a collection tube having an open end adapted to receive a quantity
of blood for separation into light and heavy phases wherein the
heavy phase precipitates to the bottom of the tube and the light
phase floats above the heavy phase;
a member adapted to be inserted into the open end of the collection
tube;
resilient sealing means associated with said member for forming a
seal between the member and an interior surface of said collection
tube when the member is inserted into the collection tube, at least
a portion of said sealing means adapted to leak when subjected to a
predetermined pressure;
filter means disposed adjacent the portion of the sealing means
adapted to leak and between the member and the interior surface of
the collection tube when the member is inserted into the collection
tube said filter means being in sealing engagement with said member
and the collection tube; and
means for inserting said member into the open end of the collection
tube and for applying a force to said member to urge the member
into the collection tube whereby a pressure exceeding the
predetermined pressure is developed in the tube and the portion of
the seal means caused to leak so that the light phase of separated
blood contained in the tube flows past the seal and through the
filter means as the member moves in a downward direction in
response to the force applied thereto, and upon termination of the
force the member stops and forms a permanent barrier between the
filtered light phase above the member and the heavy phase below the
member.
16. A separator apparatus for use with a blood collection tube to
form a permanent barrier between the separated light and heavy
phases of blood contained in the collection tube, comprising:
a member adapted to be inserted into the collection tube;
resilient sealing means associated with said member for forming a
seal between the member and an interior surface of said collection
tube when the member is inserted into the collection tube, at least
a portion of said sealing means adapted to leak when subjected to a
predetermined pressure;
filter means disposed adjacent the portion of the sealing means
adapted to leak and between the member and the interior surface of
the collection tube when the member is inserted into the collection
tube, said filter means being formed and arranged to engage both
said member and the interior surface of said collection tube;
and
means for inserting said member into the collection tube and for
exerting a force on said member for urging said member into said
collection tube.
Description
BACKGROUND OF THE INVENTION
1. Field of the Invention
The present invention relates to apparatus for separating the
phases of blood and more particularly to an apparatus for
separating blood serum or plasma from cellular material and for
filtering the serum or plasma that is separated from the cellular
material.
2. Description of the Prior Art
In normal practice a blood sample is withdrawn from a patient by
the use of an evacuated collection tube such as a VACUTAINER tube
manufactured and sold by Becton, Dickinson and Company. The
cellular portion of the blood including white blood cells, red
blood cells and platelets may be separated from the blood serum by
the use of a centrifuge in which the tube is rotated, for example,
at approximately 2,800 revolutions per minute for a period of 10
minutes. After centrifuging, the serum or plasma is completely
separated from the cellular material and a distinct interface is
observed. However, within a short time thereafter interaction
between the liquid phase and the cellular material of the blood
begins to occur and such interaction has an undesirable effect on
tests that are to be subsequently performed on both the liquid
phase and the cellular material.
Most prior art devices required that the serum or plasma be removed
from the collection tube immediately after separation and
transferred to another container. This procedure required
considerable time and also increased the possibility of
contamination of the blood sample. The procedure also necessitated
the use of two separate containers for a single sample and
therefore increased the risk of mismarking or loss of the true
identity of the blood sample especially where large numbers of
blood tests were performed simultaneously. Transfer of the serum or
plasma also increased the possibility of spillage and the resultant
exposure of laboratory personnel to infection from diseased blood.
The prior art procedure was more expensive since it required the
use of an additional container.
Thus, there was a need for an apparatus for providing a physical
separation between the serum or plasma and the cellular material of
the blood. The physical separation had to be inserted without
unduly disturbing the interface between the separated phases of the
blood while being sufficiently sturdy to allow for transportation
of the container by mail without movement of or leakage around the
separation device.
Many modern clinical laboratories are equipped with automatic blood
analyzing devices and heretofore these devices have experienced
difficulty with clogging because of the presence of fibrin
suspended in the serum or plasma phase of the blood. It therefore
became desirable that the serum or plasma be filtered to remove the
suspended fibrin or other particles.
SUMMARY OF THE INVENTION
The present invention contemplates a manually inserted serum-plasma
separator that includes a filter for removing particles from the
separated serum or plasma. The separator is designed for insertion
into a collection tube similar to the VACUTAINER type of blood
collection tube sold by Becton, Dickinson and Company. The
separator comprises a piston having two spaced annular resilient
seals. Filter material is disposed about the piston between the
spaced seals. The seals and filter material are resilient and of
such size that there is an interference fit with the interior
surface of the collection tube to establish seals between the
piston and the interior surface of the collection tube. A pusher is
detachably connected to the top of the piston so as to provide a
handle for inserting the piston into the collection tube and for
allowing the piston to be forced in a downward direction through
the collection tube.
In use the piston is inserted into the collection tube after a
blood sample has been taken and separated into its light and heavy
phases having a distinct interface therebetween. The piston is then
pushed downwardly through the light phase until it reaches a point
just above the interface. The downward movement of the piston
creates a pressure below the piston which causes the resilient
seals to temporarily deform and leak thereby allowing the serum or
plasma phase to flow in an upward direction about the piston and
through the filter material into the space above said piston. When
the bottom of the piston reaches a point just above the interface
the downward movement is terminated and the pusher is detached from
the piston and removed from the collection tube. A stopper may then
be inserted in the open end of the collection tube so that the
separated blood sample may be shipped or stored in the collection
tube for later use.
The piston, because of the interference fit, remains as a permanent
barrier between the serum or plasma and the cellular material of
the blood so that the separated blood sample may be shipped or
stored for future laboratory tests. The serum or plasma that passes
through the filter material is essentially free of fibrin strands
so that the serum may be analyzed in automated testing equipment
without fear of clogging the equipment. The piston is easily
inserted into the collection tube by the use of the attached pusher
so that the piston is not contaminated by foreign material
transferred from the fingers of the user. The piston may be readily
pushed through the serum or plasma phase by the use of the pusher
which may be easily removed from the piston so that the container
may be resealed for storage or shipment.
The primary objective of the present invention is to provide an
apparatus for permanently separating the serum or plasma phase of
blood from the cellular phase.
Another objective of the present invention is to provide a
permanent barrier between the separated phases of blood in the
original collection tube without the need for transferring a phase
to another container.
Another objective of the present invention is to provide a
serum-plasma separator that filters the separated serum or
plasma.
Another objective of the present invention is to provide an
apparatus in which a blood sample may be collected, separated and
stored or shipped for subsequent testing.
Another objective of the present invention is to provide a
separator that may be manually inserted into the original blood
collection tube for separating a centrifuged blood sample.
Another objective of the present invention is to provide a
separator that requires low insertion force so as not to disturb
the interface of the separated blood elements.
The foregoing objectives and advantages of the invention will
appear more fully hereinafter from a consideration of the detailed
description which follows, taken together with the accompanying
drawings, wherein one embodiment of the invention is illustrated by
way of example. It is to be expressly understood, however, that the
drawings are for illustrative purposes only and are not to be
considered as defining the limits of the invention.
DESCRIPTION OF THE DRAWINGS
FIG. 1 shows a collection tube filled with blood separated into its
light and heavy phases.
FIG. 2 shows a separator assembly including a pusher adapted for
insertion into the collection tube of FIG. 1.
FIG. 3 shows the separator assembly of FIG. 2 being inserted into
the collection tube of FIG. 1.
FIG. 4 shows the pusher being removed from the collection tube of
FIG. 3 while the separator remains in place.
FIG. 5 shows the collection tube of FIG. 4 with the pusher
completely removed and a stopper placed in the open end of the
collection tube.
DESCRIPTION OF THE INVENTION
Referring to FIG. 1 there is shown a collection tube 10 such as a
VACUTAINER type collection tube sold by Becton, Dickinson and
Company. Tube 10 contains blood which has previously been separated
into a light phase 11, which may be serum or plasma, and a heavy
phase 12 containing cellular material such as red blood cells,
white blood cells and platelets. A distinct interface 20 is visible
between the separated phases.
Referring now to FIG. 2 there is shown a separator assembly having
a pusher indicated generally as 13 formed of plastic or other
suitable material. The pusher may be formed in many different
configurations, as an example the configuration shown in FIG. 2 has
a substantially flat disc top portion 14 and an intermediate stem
portion 15 having an X cross-section which terminates at its lower
end in a second disc portion 16. Embedded in the lower end is a
needle 17 which is positioned along a central axis of the pusher.
The needle which is preferably made of stainless steel has a point
18 that is inserted into the center of the top of a resilient
piston 19.
The needle is used to detachably connect the piston to the pusher
so that the piston may easily be picked up, using the top portion
14 as a handle, for insertion into the open end of the collection
tube without the fingers of the user touching the piston itself.
This configuration is important since it prevents any foreign
matter or perspiration from the user's fingers from contaminating
the piston and thereafter contaminating the serum or plasma
contained in the collection tube. The analytical tests that are
performed on the serum or plasma are extremely sensitive and the
slightest amount of contamination can easily lead to erroneous test
results. In particular, the tests for sodium may be affected by the
sodium chloride in perspiration from the user's hand.
Piston 19 includes two annular resilient sealing rings 22 and 23
formed integrally therewith for effecting seals between the piston
and the interior surface of the collection tube 10. The rings are
formed of sufficient size so that they have an interference fit
with the interior surface of tube 10. The sealing rings are
preferably disposed at an angle so as to lie in a conical surface
having a vertex below the piston. Using this seal configuration the
piston may be easily slid in a downward direction for insertion
into the collection tube. The rings are formed of sufficient
thickness so that they will begin to leak when subjected to a
predetermined pressure.
A resilient filter sleeve 24 is disposed about the piston and
between the sealing rings 22 and 23. The filter sleeve should be
formed of a resilient material so that the filter material will
engage both the piston and interior surface of the tube and form a
seal therebetween. The thickness of the filter sleeve must be
somewhat larger than the space between the piston and the interior
surface of the tube so that the sleeve is slightly compressed when
inserted into the collection tube. Several materials may be used
for the filter sleeve such as felt, knitted wool or woven cloth.
Another form of filter could be made by depositing a circular band
of long strand flock onto a tacky adhesive surface of the piston,
provided the adhesive is of a type that would not have an
undesirable chemical reaction with the blood serum or plasma. A
filter could also be provided by extruding polyurethane foam in a
tubular configuration so that it could be stretched and slipped
over ring 23 and placed around piston 19. The foam may be a type
that has inter-connected cells.
In use, the collection tube 10 is provided with a blood sample in
the conventional manner and the sample is then separated into its
light and heavy phases to develop an interface as shown in FIG. 1.
The separator assembly is picked up by the disc portion 14 and the
piston is inserted into the open top of the collection tube 10. The
piston is moved in a downward direction by applying a force to disc
14 in a direction as shown by arrow 26 in FIG. 3. Since the light
phase 11 is liquid and incompressible the downward force exerted on
the piston causes a pressure to be exerted by the liquid on the
seals formed by the sealing rings 22 and 23. When the force is
sufficient to establish the predetermined pressure the sealing
rings begin to leak and allow the serum or plasma to flow in an
upward direction between the piston and the interior surface of the
collection tube. As the serum or plasma flows upwardly the piston
moves downwardly through the serum or plasma as best shown in FIG.
3. The serum or plasma flows through filter 24 so that foreign
particles and strands or portions of fibrin are removed from the
separated serum or plasma. The piston is pushed downwardly to a
position just above interface 20 at which position the downward
force is terminated. The sealing rings again engage the interior
surface of the collection tube and a permanent barrier is formed
between the light and heavy phases.
An upwardly directed force is then exerted on the pusher 13 as
shown by an arrow 27 in FIG. 4. The interference fit between the
rings and the collection tube creates a greater frictional force
than that between the needle and the piston and as a result the
piston remains in place while the needle point 18 is pulled out of
the resilient piston. The interference fit between rings 22 and 23
and the interior surface of the tube causes the piston to remain in
position as a permanent barrier between the filtered serum or
plasma and the cellular material.
It is to be noted that any other means may be used to detachably
connect the pusher to the top of the piston. For example, a
spherical bead like tip could be molded to the lower end of the
pusher and a matching spherical cavity could be formed in the
center of the top surface of the resilient piston 19. The pusher
and the piston could be snapped together during manufacture and
when the pusher is withdrawn the suction below the piston would
hold it in place while the resiliency of the piston would allow the
cavity in the top of the piston to yield permitting withdrawal of
the pusher.
It is also contemplated that the piston could be formed of a
non-resilient material such as hard rubber, plastic or metal. In
such an embodiment the pusher could be threaded into the piston or
detachably connected by other means familiar to the art. When a
non-resilient piston is used the resilient annular seal rings must
be mounted on the piston. The rings may be mounted by inserting
them in grooves formed in the piston or the rings could be
adhesively attached to the piston.
The use of two annular rings is preferred since they cooperate to
hold the filter sleeve in place; however, it is to be understood
that a single ring could be used provided the filter is held in
place by other means.
After the pusher 13 is removed, stopper 25 shown in FIG. 5 may be
inserted in the open end of the collection tube so that the
separated blood components may be stored or shipped to a laboratory
for analysis.
The invention has been described in its preferred embodiment using
a filter sleeve around the piston. Other embodiments are
contemplated wherein only a portion of the annular seal is designed
to leak at a predetermined pressure. In such an embodiment the
filter need only be disposed adjacent the portion of the seal that
leaks so as to filter the serum passing the seal. In such an
embodiment the filter would not be an annular sleeve and would
therefore be attached to the piston by a suitable adhesive.
Thus, the present invention provides an inexpensive device for
filtering and permanently separating serum or plasma from the
cellular portion of a blood sample. The separated sample may be
stored or shipped for subsequent testing. The separator device may
be used in the original collection tube and therefore eliminates
the need for a second container and also eliminates the time
required to transfer a portion of the blood into the second
container. The risk of contamination and misidentification of the
blood sample is substantially reduced by eliminating the need for
the second container. The filter portion of the separator removes
fibrin or other particles from the separated serum or plasma so
that the original collection tube may be inserted directly into
automatic blood analyzing equipment without fear of clogging the
analyzer. By providing a removable pusher the container may be
sealed so that the blood sample may be stored or shipped without
fear of the separated components interacting with each other or
being mixed together during shipment.
* * * * *