Compounds And Methods For Modulation Of Dystrophia Myotonica-protein Kinase (dmpk) Expression

Abstract

Provided herein are methods, compounds, and compositions for reducing expression of a DMPK mRNA and protein in an animal. Also provided herein are methods, compounds, and compositions for preferentially reducing CUGexp DMPK RNA, reducing myotonia or reducing spliceopathy in an animal. Such methods, compounds, and compositions are useful to treat, prevent, delay, or ameliorate type 1 myotonic dystrophy, or a symptom thereof.

Description

SEQUENCE LISTING

[0001] The present application is being filed along with a Sequence Listing in electronic format. The Sequence Listing is provided as a file entitled BIOL0171WOSEQ_ST25.txt created Aug. 1, 2014, which is approximately 276 Kb in size. The information in the electronic format of the sequence listing is incorporated herein by reference in its entirety.

FIELD

[0002] Provided herein are methods, compounds, and compositions for reducing expression of DMPK mRNA and protein in an animal. Also, provided herein are methods, compounds, and compositions comprising a DMPK inhibitor for preferentially reducing CUGexp DMPK RNA, reducing myotonia, or reducing spliceopathy in an animal. Such methods, compounds, and compositions are useful, for example, to treat, prevent, or ameliorate type 1 myotonic dystrophy (DM1) in an animal.

BACKGROUND

[0003] Myotonic dystrophy type 1 (DM1) is the most common form of muscular dystrophy in adults with an estimated frequency of 1 in 7,500 (Harper P S., Myotonic Dystrophy. London: W.B. Saunders Company; 2001). DM1 is an autosomal dominant disorder caused by expansion of a non-coding CTG repeat in DMPK1. DMPK1 is a gene encoding a cytosolic serine/threonine kinase (Brook J D, et al., Cell., 1992, 68(4):799-808). The physiologic functions and substrates of this kinase have not been fully determined. The expanded CTG repeat is located in the 3' untranslated region (UTR) of DMPK1. This mutation leads to RNA dominance, a process in which expression of RNA containing an expanded CUG repeat (CUGexp) induces cell dysfunction (Osborne R J and Thornton C A., Human Molecular Genetics., 2006, 15(2): R162-R169).

[0004] The DMPK gene normally has 5-37 CTG repeats in the 3' untranslated region. In myotonic dystrophy type I, this number is significantly expanded and is, for example, in the range of 50 to greater than 3,500 (Harper, Myotonic Dystrophy (Saunders, London, ed. 3, 2001); Annu. Rev. Neurosci. 29: 259, 2006; EMBO J. 19: 4439, 2000; Curr Opin Neurol. 20: 572, 2007).

[0005] The CUGexp tract interacts with RNA binding proteins including muscleblind-like (MBNL) protein, a splicing factor, and causes the mutant transcript to be retained in nuclear foci. The toxicity of this RNA stems from sequestration of RNA binding proteins and activation of signaling pathways. Studies in animal models have shown that phenotypes of DM1 can be reversed if toxicity of CUGexp RNA is reduced (Wheeler T M, et al., Science., 2009, 325(5938):336-339; Mulders S A, et al., Proc Natl Acad Sci USA., 2009, 106(33):13915-13920).

[0006] In DM1, skeletal muscle is the most severely affected tissue, but the disease also has important effects on cardiac and smooth muscle, ocular lens, and brain. The cranial, distal limb, and diaphragm muscles are preferentially affected. Manual dexterity is compromised early, which causes several decades of severe disability. The median age at death is 55 years, usually from respiratory failure (de Die-Smulders C E, et al., Brain., 1998, 121(Pt 8):1557-1563).

[0007] Antisense technology is emerging as an effective means for modulating expression of certain gene products and may therefore prove to be uniquely useful in a number of therapeutic, diagnostic, and research applications for the modulation of DMPK1. Intramuscular injection of fully modified oligonucleotides targeting with the CAG-repeat were shown in mice to block formation of CUGexp-MBNL1 complexes, disperse nuclear foci of CUGexp transcripts, enhance the nucleocytoplasmic transport and translation of CUGexp transcripts, release MBNL proteins to the nucleoplasm, normalize alternative splicing of MBNL-dependent exons, and eliminate myotonia in CUGexp-expressing transgenic mice (Wheeler T M, et al., Science., 2009, 325(5938):336-339; WO2008/036406).

[0008] Presently there is no treatment that can modify the course of DM1. The burden of disease, therefore, is significant. It is, therefore, an object herein to provide compounds, compositions, and methods for treating DM1

SUMMARY

[0009] Provided herein are methods, compounds, and compositions for inhibiting expression of DMPK and treating, preventing, delaying or ameliorating a DMPK related disease and or a symptom thereof. In certain embodiments, the compounds and compositions disclosed herein inhibit mutant DMPK or CUGexp DMPK.

[0010] Certain embodiments provide a method of reducing DMPK expression in an animal comprising administering to the animal a compound comprising a modified oligonucleotide as further described herein targeted to DMPK.

[0011] Certain embodiments provide a method of preferentially reducing CUGexp DMPK relative to wild-type DMPK, reducing myotonia, or reducing spliceopathy in an animal comprising administering to the animal a compound comprising a modified oligonucleotide, as further described herein, targeted to CUGexp DMPK. In certain instances, CUGexp DMPK transcripts are believed to be particularly sensitive to antisense knockdown via nuclear ribonucleases (such as RNase H), because of their longer residence time in the nucleus, and this sensitivity is thought to permit effective antisense inhibition of CUGexp DMPK transcripts in relevant tissues such as muscle despite the biodistribution barriers to tissue uptake of antisense oligonucleotides. Antisense mechanisms that do not elicit cleavage via nuclear ribonucleases, such as the CAG-repeat ASOs described in, for example, Wheeler T M, et al., Science., 2009, 325(5938):336-339 and WO2008/036406, do not provide the same therapeutic advantage.

[0012] Certain embodiments provide a method of treating an animal having type 1 myotonic dystrophy. In certain embodiments, the method includes administering to the animal a therapeutically effective amount of a compound comprising a modified oligonucleotide as further described herein targeted to DMPK. In certain embodiments, the method includes identifying an animal with type 1 myotonic dystrophy.

[0013] Certain embodiments provide a method of treating, preventing, delaying, or ameliorating symptoms and outcomes associated with development of DM1 including muscle stiffness, myotonia, disabling distal weakness, weakness in face and jaw muscles, difficulty in swallowing, drooping of the eyelids (ptosis), weakness of neck muscles, weakness in arm and leg muscles, persistent muscle pain, hypersomnia, muscle wasting, dysphagia, respiratory insufficiency, irregular heartbeat, heart muscle damage, apathy, insulin resistance, and cataracts. Certain embodiments provide a method of treating, preventing, delaying, or ameliorating symptoms and outcomes associated with development of DM1 in children, including, developmental delays, learning problems, language and speech issues, and personality development issues.

[0014] Certain embodiments provide a method of administering an antisense oligonucleotide to counteract RNA dominance by directing the cleavage of pathogenic transcripts.

[0015] In certain embodiments, the DMPK has a sequence as set forth in GenBank Accession No. NM_001081560.1 (incorporated herein as SEQ ID NO: 1). In certain embodiments, the DMPK has the sequence as set forth in GenBank Accession No. NT_011109.15 truncated from nucleotides 18540696 to Ser. No. 18/555,106 (incorporated herein as SEQ ID NO: 2). In certain embodiments, the DMPK has the sequence as set forth in GenBank Accession No. NT_039413.7 truncated from nucleotides 16666001 to Ser. No. 16/681,000 (incorporated herein as SEQ ID NO: 3). In certain embodiments, the DMPK has the sequence as set forth in GenBank Accession No. NM_032418.1 (incorporated herein as SEQ ID NO: 4). In certain embodiments, the DMPK has the sequence as set forth in GenBank Accession No. AI007148.1 (incorporated herein as SEQ ID NO: 5). In certain embodiments, the DMPK has the sequence as set forth in GenBank Accession No. AI304033.1 (incorporated herein as SEQ ID NO: 6). In certain embodiments, the DMPK has the sequence as set forth in GenBank Accession No. BC024150.1 (incorporated herein as SEQ ID NO: 7). In certain embodiments, the DMPK has the sequence as set forth in GenBank Accession No. BC056615.1 (incorporated herein as SEQ ID NO: 8). In certain embodiments, the DMPK has the sequence as set forth in GenBank Accession No. BC075715.1 (incorporated herein as SEQ ID NO: 9). In certain embodiments, the DMPK has the sequence as set forth in GenBank Accession No. BU519245.1 (incorporated herein as SEQ ID NO: 10). In certain embodiments, the DMPK has the sequence as set forth in GenBank Accession No. CB247909.1 (incorporated herein as SEQ ID NO: 11). In certain embodiments, the DMPK has the sequence as set forth in GenBank Accession No. CX208906.1 (incorporated herein as SEQ ID NO: 12). In certain embodiments, the DMPK has the sequence as set forth in GenBank Accession No. CX732022.1 (incorporated herein as SEQ ID NO: 13). In certain embodiments, the DMPK has the sequence as set forth in GenBank Accession No. S60315.1 (incorporated herein as SEQ ID NO: 14). In certain embodiments, the DMPK has the sequence as set forth in GenBank Accession No. S60316.1 (incorporated herein as SEQ ID NO: 15). In certain embodiments, the DMPK has the sequence as set forth in GenBank Accession No. NM_001081562.1 (incorporated herein as SEQ ID NO: 16). In certain embodiments, the DMPK has the sequence as set forth in GenBank Accession No. NM_001100.3 (incorporated herein as SEQ ID NO: 17).

[0016] The present disclosure provides the following non-limiting numbered embodiments:

EMBODIMENT 1

[0017] A compound comprising a modified oligonucleotide consisting of 10-30 linked nucleosides and having a nucleobase sequence comprising a complementary region comprising at least 8 contiguous nucleobases complementary to a target region of equal length of a DMPK nucleic acid.

EMBODIMENT 2

[0018] The compound of embodiment 1, wherein at least one nucleoside of the modified oligonucleotide comprises a bicyclic sugar selected from among cEt, LNA, .alpha.-L-LNA, ENA and 2'-thio LNA.

EMBODIMENT 3

[0019] The compound of any of embodiments 1 to 2, wherein the target region is exon 9 of a DMPK nucleic acid.

EMBODIMENT 4

[0020] The compound of any of embodiments 1 to 3, wherein the complementary region comprises at least 10 contiguous nucleobases complementary to a target region of equal length of a DMPK transcript.

EMBODIMENT 5

[0021] The compound of any of embodiments 1 to 3, wherein the complementary region comprises at least 12 contiguous nucleobases complementary to a target region of equal length of a DMPK nucleic acid.

EMBODIMENT 6

[0022] The compound of any of embodiments 1 to 3, wherein the complementary region comprises at least 14 contiguous nucleobases complementary to a target region of equal length of a DMPK nucleic acid.

EMBODIMENT 7

[0023] The compound of any of embodiments 1 to 3, wherein the complementary region comprises at least 16 contiguous nucleobases complementary to a target region of equal length of a DMPK nucleic acid.

EMBODIMENT 8

[0024] The compound of any of embodiments 1 to 7, wherein the DMPK nucleic acid is a DMPK pre-mRNA

EMBODIMENT 9

[0025] The compound of any of embodiments 1 to 7, wherein the DMPK nucleic acid is a DMPK mRNA.

EMBODIMENT 10

[0026] The compound of any of embodiments 1 to 9, wherein the DMPK nucleic acid has a nucleobase sequence selected from among SEQ ID NO: 1 and SEQ ID NO: 2.

EMBODIMENT 11

[0027] The compound of any of embodiments 1 to 10, wherein the modified oligonucleotide has a nucleobase sequence comprising a complementary region comprising at least 10 contiguous nucleobases complementary to a target region of equal length of SEQ ID NO: 1 or SEQ ID NO: 2.

EMBODIMENT 12

[0028] The compound of embodiments 1 to 10, wherein the modified oligonucleotide has a nucleobase sequence comprising a complementary region comprising at least 12 contiguous nucleobases complementary to a target region of equal length of SEQ ID NO: 1 or SEQ ID NO: 2.

EMBODIMENT 13

[0029] The compound of embodiments 1 to 10, wherein the modified oligonucleotide has a nucleobase sequence comprising a complementary region comprising at least 14 contiguous nucleobases complementary to a target region of equal length of SEQ ID NO: 1 or SEQ ID NO: 2.

EMBODIMENT 14

[0030] The compound of embodiments 1 to 10, wherein the modified oligonucleotide has a nucleobase sequence comprising a complementary region comprising at least 16 contiguous nucleobases complementary to a target region of equal length of SEQ ID NO: 1 or SEQ ID NO: 2.

EMBODIMENT 15

[0031] The compound of any of embodiments 1 to 14, wherein the target region is from nucleobase 1343 to nucleobase 1368 of SEQ ID NO.: 1.

EMBODIMENT 16

[0032] The compound of any of embodiments 1 to 14, wherein the target region is from nucleobase 1317 to nucleobase 1366 of SEQ ID NO.: 1.

EMBODIMENT 17

[0033] The compound of any of embodiments 1 to 14, wherein the target region is from nucleobase 2748 to nucleobase 2791 of SEQ ID NO.: 1.

EMBODIMENT 18

[0034] The compound of any of embodiments 1 to 14, wherein the target region is from nucleobase 730 to nucleobase 748 of SEQ ID NO.: 1.

EMBODIMENT 19

[0035] The compound of any of embodiments 1 to 14, wherein the target region is from nucleobase 10195 to nucleobase 10294 of SEQ ID NO.: 2.

EMBODIMENT 20

[0036] The compound of any of embodiments 1 to 14, wherein the target region is from nucleobase 10195 to nucleobase 10294 of SEQ ID NO.: 2.

EMBODIMENT 21

[0037] The compound of any of embodiments 1 to 14, wherein the target region is from nucleobase 10201 to nucleobase 10216 of SEQ ID NO.: 2.

EMBODIMENT 22

[0038] The compound of any of embodiments 1 to 14, wherein the target region is from nucleobase 10202 to nucleobase 10218 of SEQ ID NO.: 2.

EMBODIMENT 23

[0039] The compound of any of embodiments 1 to 22, wherein the modified oligonucleotide has a nucleobase sequence that is at least 80% complementary to the target region over the entire length of the oligonucleotide.

EMBODIMENT 24

[0040] The compound of any of embodiments 1 to 22, wherein the modified oligonucleotide has a nucleobase sequence that is at least 90% complementary to the target region over the entire length of the oligonucleotide.

EMBODIMENT 25

[0041] The compound of any of embodiments 1 to 22, wherein the modified oligonucleotide has a nucleobase sequence that is at least 100% complementary to the target region over the entire length of the oligonucleotide.

EMBODIMENT 26

[0042] The compound of any of embodiments 1-25 having a nucleobase sequence comprising at least 8 contiguous nucleobases of a sequence recited in any of SEQ ID NOs: 23-874.

EMBODIMENT 27

[0043] The compound of any of embodiments 1 to 25, wherein the modified oligonucleotide has a nucleobase sequence comprising at least 10 contiguous nucleobases of sequence recited in SEQ ID NOs: 23-32.

EMBODIMENT 28

[0044] The compound of any of embodiments 1 to 25, wherein the modified oligonucleotide has a nucleobase sequence comprising at least 12 contiguous nucleobases of sequence recited in SEQ ID NOs: 23-32.

EMBODIMENT 29

[0045] The compound of any of embodiments 1 to 25, wherein the modified oligonucleotide has a nucleobase sequence comprising at least 14 contiguous nucleobases of sequence recited in SEQ ID NOs: 23-32.

EMBODIMENT 30

[0046] The compound of any of embodiments 1 to 25, wherein the modified oligonucleotide has a nucleobase sequence comprising at least 16 contiguous nucleobases of sequence recited in SEQ ID NOs: 23-32.

EMBODIMENT 31

[0047] The compound of any of embodiments 1 to 30, wherein the modified oligonucleotide has a nucleobase sequence that consists of the sequence recited in SEQ ID NO: 23.

EMBODIMENT 32

[0048] The compound of any of embodiments 1 to 14, wherein the modified oligonucleotide has a nucleobase sequence that consists of the sequence recited in SEQ ID NO: 25.

EMBODIMENT 33

[0049] The compound of any of embodiments 1 to 14, wherein the modified oligonucleotide has a nucleobase sequence that consists of the sequence recited in SEQ ID NO: 26.

EMBODIMENT 34

[0050] The compound of any of embodiments 1 to 14, wherein the modified oligonucleotide has a nucleobase sequence that consists of the sequence recited in SEQ ID NO: 27.

EMBODIMENT 35

[0051] The compound of any of embodiments 1 to 14, wherein the modified oligonucleotide has a nucleobase sequence that consists of the sequence recited in SEQ ID NO: 28.

EMBODIMENT 36

[0052] The compound of any of embodiments 1 to 14, wherein the modified oligonucleotide has a nucleobase sequence that consists of the sequence recited in SEQ ID NO: 29.

EMBODIMENT 37

[0053] The compound of any of embodiments 1 to 14, wherein the modified oligonucleotide has a nucleobase sequence that consists of the sequence recited in SEQ ID NO: 30.

EMBODIMENT 38

[0054] The compound of any of embodiments 1 to 14, wherein the modified oligonucleotide has a nucleobase sequence that consists of the sequence recited in SEQ ID NO: 31.

EMBODIMENT 39

[0055] The compound of any of embodiments 1 to 14, wherein the modified oligonucleotide has a nucleobase sequence that consists of the sequence recited in SEQ ID NO: 32.

EMBODIMENT 40

[0056] The compound of any of embodiments 1 to 14, wherein the modified oligonucleotide has a nucleobase sequence comprising the sequence recited in SEQ ID NO: 23, 24, 25, 26, 27, 28, 29, 30, 31, or 32.

EMBODIMENT 41

[0057] The compound of any of embodiments 1 to 14, wherein the modified oligonucleotide has a nucleobase sequence comprising the sequence recited in SEQ ID NO: 23, 25, 26, 27, 28, 29, 30, 31, or 32.

EMBODIMENT 42

[0058] The compound of any of embodiments 1 to 14, wherein the modified oligonucleotide has a nucleobase sequence comprising the sequence recited in SEQ ID NO: 33-874.

EMBODIMENT 43

[0059] The compound of any of embodiments 1 to 42, wherein the nucleobase sequence of the modified oligonucleotide is at least 90% complementary to SEQ ID NOs: 1-19.

EMBODIMENT 44

[0060] The compound of any of embodiments 1 to 34, wherein the nucleobase sequence of the modified oligonucleotide is 100% complementary to SEQ ID NOs: 1-19.

EMBODIMENT 45

[0061] The compound of any of embodiments 1 to 30, wherein the modified oligonucleotide consists of 16 linked nucleosides.

EMBODIMENT 46

[0062] The compound of any of embodiments 1 to 30, wherein the modified oligonucleotide consists of 17 linked nucleosides.

EMBODIMENT 47

[0063] The compound of any of embodiments 1 to 30, wherein the modified oligonucleotide consists of 18 linked nucleosides.

EMBODIMENT 48

[0064] The compound of any of embodiments 1 to 30, wherein the modified oligonucleotide consists of 19 linked nucleosides.

EMBODIMENT 49

[0065] The compound of any of embodiments 1 to 30, wherein the modified oligonucleotide consists of 20 linked nucleosides.

EMBODIMENT 50

[0066] The compound of any of embodiments 1 to 49, wherein the modified oligonucleotide is a single-stranded oligonucleotide.

EMBODIMENT 51

[0067] The compound of any of embodiments 1 to 50 wherein at least one nucleoside comprises a modified sugar.

EMBODIMENT 52

[0068] The compound of any of embodiments 1 to 51 wherein at least two nucleosides comprise a modified sugar.

EMBODIMENT 53

[0069] The compound of embodiment 52, wherein each of the modified sugars have the same modification.

EMBODIMENT 54

[0070] The compound of embodiment 52, wherein at least one the modified sugars has a different modification.

EMBODIMENT 55

[0071] The compound of any of embodiments 51 to 54, wherein at least one modified sugar is a bicyclic sugar.

EMBODIMENT 56

[0072] The compound of embodiment 55, wherein the bicyclic sugar is selected from among cEt, LNA, .alpha.-L-LNA, ENA and 2'-thio LNA.

EMBODIMENT 57

[0073] The compound of embodiment 56, wherein the bicyclic sugar comprises cEt.

EMBODIMENT 58

[0074] The compound of embodiment 56, wherein the bicyclic sugar comprises LNA.

EMBODIMENT 59

[0075] The compound of embodiment 56, wherein the bicyclic sugar comprises .alpha.-L-LNA.

EMBODIMENT 60

[0076] The compound of embodiment 56, wherein the bicyclic sugar comprises ENA.

EMBODIMENT 61

[0077] The compound of embodiment 56, wherein the bicyclic sugar comprises 2'-thio LNA.

EMBODIMENT 62

[0078] The compound of any of embodiments 1 to 61, wherein at least one modified sugar comprises a 2'-substituted nucleoside.

EMBODIMENT 63

[0079] The compound of embodiment 62, wherein the 2'-substituted nucleoside is selected from among: 2'-OCH.sub.3, 2'-F, and 2'-O-methoxyethyl.

EMBODIMENT 64

[0080] The compound of any of embodiments 1 to 63, wherein at least one modified sugar comprises a 2'-O-methoxyethyl.

EMBODIMENT 65

[0081] The compound of any of embodiments 1 to 64, wherein at least one nucleoside comprises a modified nucleobase.

EMBODIMENT 66

[0082] The compound of embodiment 65, wherein the modified nucleobase is a 5-methylcytosine.

EMBODIMENT 67

[0083] The compound of any of embodiments 1 to 67, wherein each cytosine is a 5-methylcytosine.

EMBODIMENT 68

[0084] The compound of any of embodiments 1 to 67, wherein the modified oligonucleotide comprises: [0085] a. a gap segment consisting of linked deoxynucleosides; [0086] b. a 5' wing segment consisting of linked nucleosides; [0087] c. a 3' wing segment consisting of linked nucleosides; [0088] d. wherein the gap segment is positioned between the 5' wing segment and the 3' wing segment and wherein each nucleoside of each wing segment comprises a modified sugar.

EMBODIMENT 69

[0089] The compound of embodiment 68, wherein the modified oligonucleotide consists of 16 linked nucleosides.

EMBODIMENT 70

[0090] The compound of embodiment 68, wherein the modified oligonucleotide consists of 17 linked nucleosides.

EMBODIMENT 71

[0091] The compound of embodiment 68, wherein the modified oligonucleotide consists of 18 linked nucleosides.

EMBODIMENT 72

[0092] The compound of embodiment 68, wherein the modified oligonucleotide consists of 19 linked nucleosides.

EMBODIMENT 73

[0093] The compound of embodiment 68, wherein the modified oligonucleotide consists of 20 linked nucleosides.

EMBODIMENT 74

[0094] The compound of any of embodiments 68 to 73, wherein the 5'-wing segment consists of two linked nucleosides.

EMBODIMENT 75

[0095] The compound of any of embodiments 68 to 73, wherein the 5'-wing segment consists of three linked nucleosides.

EMBODIMENT 76

[0096] The compound of any of embodiments 68 to 73, wherein the 5'-wing segment consists of four linked nucleosides.

EMBODIMENT 77

[0097] The compound of any of embodiments 68 to 73, wherein the 5'-wing segment consists of five linked nucleosides.

EMBODIMENT 78

[0098] The compound of any of embodiments 68 to 73, wherein the 5'-wing segment consists of six linked nucleosides.

EMBODIMENT 79

[0099] The compound of any of embodiments 68 to 78, wherein the 3'-wing segment consists of two linked nucleosides.

EMBODIMENT 80

[0100] The compound of any of embodiments 68 to 78, wherein the 3'-wing segment consists of three linked nucleosides.

EMBODIMENT 81

[0101] The compound of any of embodiments 68 to 78, wherein the 3'-wing segment consists of four linked nucleosides.

EMBODIMENT 82

[0102] The compound of any of embodiments 68 to 78, wherein the 3'-wing segment consists of five linked nucleosides.

EMBODIMENT 83

[0103] The compound of any of embodiments 68 to 78, wherein the 3'-wing segment consists of six linked nucleosides.

EMBODIMENT 84

[0104] The compound of any of embodiments 68 to 83, wherein the gap segment consists of six linked deoxynucleosides.

EMBODIMENT 85

[0105] The compound of any of embodiments 68 to 83, wherein the gap segment consists of seven linked deoxynucleosides.

EMBODIMENT 86

[0106] The compound of any of embodiments 68 to 83, wherein the gap segment consists of eight linked deoxynucleosides.

EMBODIMENT 87

[0107] The compound of any of embodiments 68 to 83, wherein the gap segment consists of nine linked deoxynucleosides.

EMBODIMENT 88

[0108] The compound of any of embodiments 68 to 83, wherein the gap segment consists of ten linked deoxynucleosides.

EMBODIMENT 89

[0109] The compound of any of embodiments 1 to 31, 34, 37 to 45, or 53 to 88, wherein the modified oligonucleotide consists of 16 linked nucleosides and comprises: [0110] a. a gap segment consisting of ten linked deoxynucleosides; [0111] b. a 5' wing segment consisting of three linked nucleosides; [0112] c. a 3' wing segment consisting of three linked nucleosides; [0113] d. wherein the gap segment is positioned between the 5' wing segment and the 3' wing segment, and wherein each nucleoside of each wing segment comprises a bicyclic sugar.

EMBODIMENT 90

[0114] The compound of any of embodiments 1 to 31, 34, 37 to 45, or 53 to 88, wherein the modified oligonucleotide consists of 16 linked nucleosides and comprises: [0115] a. a gap segment consisting of eight linked deoxynucleosides; [0116] b. a 5' wing segment consisting of four linked nucleosides and having an AABB 5'-wing motif; [0117] c. a 3' wing segment consisting of four linked nucleosides and having a BBAA 3'-wing motif; [0118] d. wherein the gap segment is positioned between the 5' wing segment and the 3' wing segment.

EMBODIMENT 91

[0119] The compound of any of embodiments 1 to 30, 35, 36, 46, or 50 to 88, wherein the modified oligonucleotide consists of 17 linked nucleosides and comprises: [0120] a. a gap segment consisting of seven linked deoxynucleosides; [0121] b. a 5' wing segment consisting of five linked nucleosides and having an AAABB 5'-wing motif; [0122] c. a 3' wing segment consisting of five linked nucleosides and having a BBAAA 3'-wing motif; [0123] d. wherein the gap segment is positioned between the 5' wing segment and the 3' wing segment.

EMBODIMENT 92

[0124] The compound of any of embodiments 1 to 31, 34, 37 to 45, or 53 to 88, wherein the modified oligonucleotide consists of 16 linked nucleosides and comprises: [0125] a. a gap segment consisting of eight linked deoxynucleosides; [0126] b. a 5' wing segment consisting of four linked nucleosides and having a E-E-K-K 5'-wing motif; [0127] c. a 3' wing segment consisting of four linked nucleosides and having a K-K-E-E 3'-wing motif; [0128] d. wherein the gap segment is positioned between the 5' wing segment and the 3' wing segment, and wherein each E represents 2'-O-methoxyethyl sugar and each K represents a cEt sugar.

EMBODIMENT 93

[0129] The compound of any of embodiments 1 to 30, 35, 36, 46, or 50 to 88, wherein the modified oligonucleotide consists of 17 linked nucleosides and comprises: [0130] a. a gap segment consisting of seven linked deoxynucleosides; [0131] b. a 5' wing segment consisting of five linked nucleosides and having an E-E-E-K-K 5'-wing motif; [0132] c. a 3' wing segment consisting of five linked nucleosides and having a K-K-E-E-E 3'-wing motif; [0133] d. wherein the gap segment is positioned between the 5' wing segment and the 3' wing segment, and wherein each E represents 2'-O-methoxyethyl sugar and each K represents a cEt sugar.

EMBODIMENT 94

[0134] The compound of any of embodiments 1 to 30, 32, 33, or 49 to 88, wherein the modified oligonucleotide consists of 20 linked nucleosides and comprises: [0135] a. a gap segment consisting of ten linked deoxynucleosides; [0136] b. a 5' wing segment consisting of five linked nucleosides; [0137] c. a 3' wing segment consisting of five linked nucleosides; [0138] d. wherein the gap segment is positioned between the 5' wing segment and the 3' wing segment, and wherein each nucleoside of each wing segment comprises a 2'-O-methoxyethyl sugar.

EMBODIMENT 95

[0139] The compound of any of embodiments 1 to 31, 34, 37 to 45, or 53 to 88, wherein the modified oligonucleotide consists of 16 linked nucleosides and comprises: [0140] a. a gap segment consisting of ten linked deoxynucleosides; [0141] b. a 5' wing segment consisting of three linked nucleosides; [0142] c. a 3' wing segment consisting of three linked nucleosides; [0143] d. wherein the gap segment is positioned between the 5' wing segment and the 3' wing segment, and wherein each nucleoside of each wing segment comprises a cEt sugar.

EMBODIMENT 96

[0144] The compound of any of embodiments 1 to 67, wherein the modified oligonucleotide comprises at least 8 contiguous nucleobases complementary to a target region within nucleobase 1343 and nucleobase 1368 of SEQ ID NO.: 1, and wherein the modified oligonucleotide comprises: [0145] a. a gap segment consisting of linked deoxynucleosides; [0146] b. a 5' wing segment consisting of linked nucleosides; [0147] c. a 3' wing segment consisting of linked nucleosides; [0148] d. wherein the gap segment is positioned between the 5' wing segment and the 3' wing segment and wherein each nucleoside of each wing segment comprises a modified sugar.

EMBODIMENT 97

[0149] The compound of embodiment 96, wherein each modified sugar in the 5'-wing segment has the same modifications.

EMBODIMENT 98

[0150] The compound of embodiment 96, wherein at least two modified sugars in the 5'-wing segment have different modifications.

EMBODIMENT 99

[0151] The compound of any of embodiments 96 to 98 wherein each modified sugar in the 3'-wing segment has the same modifications.

EMBODIMENT 100

[0152] The compound of any of embodiments 96 to 98, wherein at least two modified sugars in the 3'-wing segment have different modification.

EMBODIMENT 101

[0153] The compound of embodiment 96, wherein at least one modified sugar is a bicyclic sugar selected from among cEt, LNA, .alpha.-L-LNA, ENA and 2'-thio LNAs.

EMBODIMENT 102

[0154] The compound of embodiment 90 to 91, wherein each B represents a bicyclic sugar selected from among cEt, LNA, .alpha.-L-LNA, ENA and 2'-thio LNA.

EMBODIMENT 103

[0155] The compound of embodiment 102, wherein the bicyclic sugar comprises BNA.

EMBODIMENT 104

[0156] The compound of embodiment 102, wherein the bicyclic sugar comprises cEt.

EMBODIMENT 105

[0157] The compound of embodiment 102, wherein the bicyclic sugar comprises LNA.

EMBODIMENT 106

[0158] The compound of embodiment 102, wherein the bicyclic sugar comprises .alpha.-L-LNA.

EMBODIMENT 107

[0159] The compound of embodiment 102, wherein the bicyclic sugar comprises ENA.

EMBODIMENT 108

[0160] The compound of embodiment 102, wherein the bicyclic sugar comprises 2'-thio LNA.

EMBODIMENT 109

[0161] The compound of embodiment 90 or 91, wherein each A represents a 2'-substituted nucleoside is selected from among: 2'-OCH.sub.3, 2'-F, and 2'-O-methoxyethyl.

EMBODIMENT 110

[0162] The compound of embodiment 109, wherein the 2'-substituted nucleoside comprises 2'-O-methoxyethyl.

EMBODIMENT 111

[0163] The compound of any of embodiments 1 to 111, wherein at least one internucleoside linkage is a modified internucleoside linkage.

EMBODIMENT 112

[0164] The compound of any of embodiments 1 to 111, wherein each internucleoside linkage is a phosphorothioate internucleoside linkage.

EMBODIMENT 113

[0165] A compound consisting of ISIS 486178.

EMBODIMENT 114

[0166] A compound consisting of ISIS 512497.

EMBODIMENT 115

[0167] A compound consisting of ISIS 598768.

EMBODIMENT 116

[0168] A compound consisting of ISIS 594300.

EMBODIMENT 117

[0169] A compound consisting of ISIS 594292.

EMBODIMENT 118

[0170] A compound consisting of ISIS 569473.

EMBODIMENT 119

[0171] A compound consisting of ISIS 598769.

EMBODIMENT 120

[0172] A compound consisting of ISIS 570808.

EMBODIMENT 121

[0173] A compound consisting of ISIS 598777.

EMBODIMENT 122

[0174] A compound having a nucleobase sequence as set forth in SEQ ID NO: 23, wherein the modified oligonucleotide consists of 16 linked nucleosides and comprises: [0175] a. a gap segment consisting of ten linked deoxynucleosides; [0176] b. a 5' wing segment consisting of three linked nucleosides; [0177] c. a 3' wing segment consisting of three linked nucleosides; [0178] d. wherein the gap segment is positioned between the 5' wing segment and the 3' wing segment; [0179] e. wherein each nucleoside of each wing segment comprises a bicyclic sugar; [0180] f. wherein each internucleoside linkage is a phosphorothioate internucleoside linkage; and [0181] g. wherein each cytosine residue is a 5-methyl cytosine.

EMBODIMENT 123

[0182] A compound having a nucleobase sequence as set forth in SEQ ID NO: 29, wherein the modified oligonucleotide consists of 16 linked nucleosides and comprises: [0183] a. a gap segment consisting of ten linked deoxynucleosides; [0184] b. a 5' wing segment consisting of three linked nucleosides; [0185] c. a 3' wing segment consisting of three linked nucleosides; [0186] d. wherein the gap segment is positioned between the 5' wing segment and the 3' wing segment; [0187] e. wherein each nucleoside of each wing segment comprises a bicyclic sugar; [0188] f. wherein each internucleoside linkage is a phosphorothioate internucleoside linkage; and [0189] g. wherein each cytosine residue is a 5-methyl cytosine.

EMBODIMENT 124

[0190] A compound having a nucleobase sequence as set forth in SEQ ID NO: 31, wherein the modified oligonucleotide consists of 16 linked nucleosides and comprises: [0191] a. a gap segment consisting of ten linked deoxynucleosides; [0192] b. a 5' wing segment consisting of three linked nucleosides; [0193] c. a 3' wing segment consisting of three linked nucleosides; [0194] d. wherein the gap segment is positioned between the 5' wing segment and the 3' wing segment; [0195] e. wherein each nucleoside of each wing segment comprises a bicyclic sugar; [0196] f. wherein each internucleoside linkage is a phosphorothioate internucleoside linkage; and [0197] g. wherein each cytosine residue is a 5-methyl cytosine.

EMBODIMENT 125

[0198] A compound having a nucleobase sequence as set forth in SEQ ID NO: 26, wherein the modified oligonucleotide consists of 16 linked nucleosides and comprises: [0199] a. a gap segment consisting of eight linked deoxynucleosides; [0200] b. a 5' wing segment consisting of four linked nucleosides and having a E-E-K-K 5'-wing motif; [0201] c. a 3' wing segment consisting of four linked nucleosides and having a K-K-E-E 3'-wing motif; [0202] d. wherein the gap segment is positioned between the 5' wing segment and the 3' wing segment; [0203] e. wherein each E represents 2'-O-methoxyethyl sugar and each K represents a cEt sugar; [0204] f. wherein each internucleoside linkage is a phosphorothioate internucleoside linkage; and [0205] g. wherein each cytosine residue is a 5-methyl cytosine.

EMBODIMENT 126

[0206] A compound having a nucleobase sequence as set forth in SEQ ID NO: 30, wherein the modified oligonucleotide consists of 16 linked nucleosides and comprises: [0207] a. a gap segment consisting of eight linked deoxynucleosides; [0208] b. a 5' wing segment consisting of four linked nucleosides and having a E-E-K-K 5'-wing motif; [0209] c. a 3' wing segment consisting of four linked nucleosides and having a K-K-E-E 3'-wing motif; [0210] d. wherein the gap segment is positioned between the 5' wing segment and the 3' wing segment; [0211] e. wherein each E represents 2'-O-methoxyethyl sugar and each K represents a cEt sugar; [0212] f. wherein each internucleoside linkage is a phosphorothioate internucleoside linkage; and [0213] g. wherein each cytosine residue is a 5-methyl cytosine.

EMBODIMENT 127

[0214] A compound having a nucleobase sequence as set forth in SEQ ID NO: 32, wherein the modified oligonucleotide consists of 16 linked nucleosides and comprises: [0215] a. a gap segment consisting of eight linked deoxynucleosides; [0216] b. a 5' wing segment consisting of four linked nucleosides and having a E-E-K-K 5'-wing motif; [0217] c. a 3' wing segment consisting of four linked nucleosides and having a K-K-E-E 3'-wing motif; [0218] d. wherein the gap segment is positioned between the 5' wing segment and the 3' wing segment; [0219] e. wherein each E represents 2'-O-methoxyethyl sugar and each K represents a cEt sugar; [0220] f. wherein each internucleoside linkage is a phosphorothioate internucleoside linkage; and [0221] g. wherein each cytosine residue is a 5-methyl cytosine.

EMBODIMENT 128

[0222] A compound having a nucleobase sequence as set forth in SEQ ID NO: 27, wherein the modified oligonucleotide consists of 17 linked nucleosides and comprises: [0223] a. a gap segment consisting of seven linked deoxynucleosides; [0224] b. a 5' wing segment consisting of five linked nucleosides and having an E-E-E-K-K 5'-wing motif; [0225] c. a 3' wing segment consisting of five linked nucleosides and having a K-K-E-E-E 3'-wing motif; [0226] d. wherein the gap segment is positioned between the 5' wing segment and the 3' wing segment; [0227] e. wherein each E represents 2'-O-methoxyethyl sugar and each K represents a cEt sugar; [0228] f. wherein each internucleoside linkage is a phosphorothioate internucleoside linkage; and [0229] g. wherein each cytosine residue is a 5-methyl cytosine.

EMBODIMENT 129

[0230] A compound having a nucleobase sequence as set forth in SEQ ID NO: 28, wherein the modified oligonucleotide consists of 17 linked nucleosides and comprises: [0231] a. a gap segment consisting of seven linked deoxynucleosides; [0232] b. a 5' wing segment consisting of five linked nucleosides and having an E-E-E-K-K 5'-wing motif; [0233] c. a 3' wing segment consisting of five linked nucleosides and having a K-K-E-E-E 3'-wing motif; [0234] d. wherein the gap segment is positioned between the 5' wing segment and the 3' wing segment; [0235] e. wherein each E represents 2'-O-methoxyethyl sugar and each K represents a cEt sugar; [0236] f. wherein each internucleoside linkage is a phosphorothioate internucleoside linkage; and [0237] g. wherein each cytosine residue is a 5-methyl cytosine.

EMBODIMENT 130

[0238] A compound having a nucleobase sequence as set forth in SEQ ID NO: 25, wherein the modified oligonucleotide consists of 20 linked nucleosides and comprises: [0239] a. a gap segment consisting of ten linked deoxynucleosides; [0240] b. a 5' wing segment consisting of five linked nucleosides; [0241] c. a 3' wing segment consisting of five linked nucleosides; [0242] d. wherein the gap segment is positioned between the 5' wing segment and the 3' wing segment; [0243] e. wherein each nucleoside of each wing segment comprises a 2'-O-methoxyethyl sugar; [0244] f. wherein each internucleoside linkage is a phosphorothioate internucleoside linkage; and [0245] g. wherein each cytosine residue is a 5-methyl cytosine.

EMBODIMENT 131

[0246] The compound of any of embodiments 1 to 130 comprising a conjugate.

EMBODIMENT 132

[0247] A composition comprising the compound of any of embodiments 1 to 131, and a pharmaceutically acceptable carrier or diluent.

EMBODIMENT 133

[0248] A method of treating DM1 in an animal comprising administering to an animal in need thereof a compound according to any of embodiments 1 to 130, or a composition according to embodiment 132.

EMBODIMENT 134

[0249] The method of embodiment 133, wherein the compound reduces DMPK mRNA levels.

EMBODIMENT 135

[0250] The method of embodiment 133, wherein the compound reduces DMPK protein expression.

EMBODIMENT 136

[0251] The method of embodiment 133, wherein the compound reduces CUGexp DMPK.

EMBODIMENT 137

[0252] The method of embodiment 133, wherein the compound preferentially reduces CUGexp DMPK.

EMBODIMENT 138

[0253] The method of embodiment 133, wherein the compound reduces CUGexp DMPK mRNA.

EMBODIMENT 139

[0254] The method of embodiment 133, wherein the compound preferentially reduces CUGexp DMPK mRNA.

EMBODIMENT 140

[0255] The method of embodiment 138 or 139, wherein the preferential reduction of CUGexp is in muscle tissue.

EMBODIMENT 141

[0256] A method of reducing myotonia in an animal comprising administering to an animal in need thereof a compound according to any of embodiments 1 to 131, or a composition according to embodiment 132.

EMBODIMENT 142

[0257] A method of reducing MBLN dependent spliceopathy in an animal comprising administering to an animal in need thereof a compound according to any of embodiments 1 to 131, or a composition according to embodiment 132.

EMBODIMENT 143

[0258] The method of embodiment 138, wherein splicing of any of Serca1, m-Titin, Clcn1, and Zasp is corrected.

EMBODIMENT 144

[0259] The method of any of embodiments 133 to 143, wherein the administering is systemic administration.

EMBODIMENT 145

[0260] The method of any of embodiments 133 to 143, wherein the administering is parenteral administration.

EMBODIMENT 146

[0261] The method of embodiment 144, wherein the systemic administration is any of subcutaneous administration, intravenous administration, intracerebroventricular administration, and intrathecal administration.

EMBODIMENT 147

[0262] The method of any of embodiments 133 to 143, wherein the administration is not intramuscular administration.

EMBODIMENT 148

[0263] The method of any of embodiments 133 to 143, wherein the animal is a human.

EMBODIMENT 149

[0264] A method of reducing spliceopathy of Serca1 in an animal in need thereof by administering a compound according to any of embodiments 1 to 131, or a composition according to embodiment 132, and thereby causing Serca1 exon 22 inclusion.

EMBODIMENT 150

[0265] A method of reducing spliceopathy of m-Titin in an animal in need thereof by administering a compound according to any of embodiments 1 to 131, or a composition according to embodiment 132, and thereby causing m-Titin exon 5 inclusion.

EMBODIMENT 151

[0266] A method of reducing spliceopathy of Clcn1 in an animal in need thereof by administering a compound according to any of embodiments 1 to 131, or a composition according to embodiment 132, and thereby causing Clcn1 exon 7a inclusion.

EMBODIMENT 152

[0267] A method of reducing spliceopathy of Zasp in an animal in need thereof by administering a compound according to any of embodiments 1 to 131, or a composition according to embodiment 132, and thereby causing Zasp exon 11 inclusion.

EMBODIMENT 153

[0268] A method of reducing DMPK mRNA in a cell, comprising contacting a cell with a compound according to any of embodiments 1 to 131, or a composition according to embodiment 132.

EMBODIMENT 154

[0269] A method of reducing DMPK protein in a cell, comprising contacting a cell with a compound according to any of embodiments 1 to 131, or a composition according to embodiment 132.

EMBODIMENT 155

[0270] A method of reducing CUGexp mRNA in a cell, comprising contacting a cell with a compound according to any of embodiments 1 to 131, or a composition according to embodiment 132.

EMBODIMENT 156

[0271] The method of any of embodiments 149 to 151, wherein the cell is in an animal.

EMBODIMENT 157

[0272] The method of embodiment 156, wherein the animal is a human.

EMBODIMENT 158

[0273] A method of achieving a preferential reduction of CUGexp DMPK RNA, comprising: [0274] a. selecting a subject having type 1 myotonic dystrophy or having a CUGexp DMPK RNA; and [0275] b. administering to said subject a compound according to any of embodiments 1 to 131, or a composition according to embodiment 132; [0276] wherein said compound according to any of embodiments 1 to 131, or a composition according to embodiment 132, when bound to said CUGexp DMPK RNA, activates a ribonuclease, thereby achieving a preferential reduction of said CUGexp DMPK RNA.

EMBODIMENT 159

[0277] A method of achieving a preferential reduction of CUGexp DMPK RNA, comprising: [0278] a. selecting a subject having type 1 myotonic dystrophy or having a CUGexp DMPK RNA; and [0279] b. systemically administering to said subject a compound according to any of embodiments 1 to 131, or a composition according to embodiment 132; [0280] wherein said chemically-modified antisense oligonucleotide, when bound to said CUGexp DMPK RNA, achieves a preferential reduction of said CUGexp DMPK RNA.

EMBODIMENT 160

[0281] A method of reducing spliceopathy in a subject suspected of having type 1 myotonic dystrophy or having a nuclear retained CUGexp DMPK RNA, comprising: [0282] administering to said subject a compound according to any of embodiments 1 to 131, or a composition according to embodiment 132, [0283] wherein the compound according to any of embodiments 1 to 131, or a composition according to embodiment 132, when bound to said mutant DMPK RNA, activates a ribonuclease, thereby reducing spliceopathy.

EMBODIMENT 161

[0284] A method of preferentially reducing CUGexp DMPK RNA, reducing myotonia or reducing spliceopathy in an animal comprising administering to the animal a compound according to any of embodiments 1 to 131 or a pharmaceutical composition of embodiment 132, wherein the compound reduces DMPK expression in the animal, thereby preferentially reducing CUGexp DMPK RNA, reducing myotonia, or reducing spliceopathy in the animal.

EMBODIMENT 162

[0285] A method for treating an animal with type 1 myotonic dystrophy comprising identifying said animal with type 1 myotonic dystrophy, [0286] administering to said animal a therapeutically effective amount of a compound according to any of embodiments 1 to 131 or a pharmaceutical composition of embodiment 132, [0287] wherein said animal with type 1 myotonic dystrophy is treated.

EMBODIMENT 163

[0288] A method of reducing DMPK expression comprising administering to an animal a compound according to any of embodiments 1 to 131 or a pharmaceutical composition of embodiment 132, wherein expression of DMPK is reduced.

EMBODIMENT 164

[0289] A compound according to any of embodiments 1 to 131 or a pharmaceutical composition of embodiment 132, for use in treating DM1 in an animal.

EMBODIMENT 165

[0290] A compound according to any of embodiments 1 to 131 or a pharmaceutical composition of embodiment 132, for use in reducing myotonia in an animal.

EMBODIMENT 166

[0291] A compound according to any of embodiments 1 to 131 or a pharmaceutical composition of embodiment 132, for use in reducing MBLN dependent spliceopathy in an animal.

DETAILED DESCRIPTION

[0292] It is to be understood that both the foregoing general description and the following detailed description are exemplary and explanatory only and are not restrictive of the invention, as claimed. Herein, the use of the singular includes the plural unless specifically stated otherwise. Herein, the use of "or" means "and/or" unless stated otherwise. Furthermore, the use of the term "including" as well as other forms, such as "includes" and "included", is not limiting. Also, terms such as "element" or "component" encompass both elements and components comprising one unit and elements and components that comprise more than one subunit, unless specifically stated otherwise.

[0293] The section headings used herein are for organizational purposes only and are not to be construed as limiting the subject matter described. All documents, or portions of documents, cited in this application, including, but not limited to, patents, patent applications, articles, books, and treatises, are hereby expressly incorporated-by-reference for the portions of the document discussed herein, as well as in their entirety.

DEFINITIONS

[0294] Unless specific definitions are provided, the nomenclature utilized in connection with, and the procedures and techniques of, analytical chemistry, synthetic organic chemistry, and medicinal and pharmaceutical chemistry described herein are those well known and commonly used in the art. Standard techniques can be used for chemical synthesis, and chemical analysis. Where permitted, all patents, applications, published applications and other publications, GENBANK Accession Numbers and associated sequence information obtainable through databases such as National Center for Biotechnology Information (NCBI) and other data referred to throughout in the disclosure herein are incorporated by reference for the portions of the document discussed herein, as well as in their entirety.

[0295] Unless otherwise indicated, the following terms have the following meanings:

[0296] "2'-O-methoxyethyl" (also 2'-MOE and 2'-O(CH.sub.2).sub.2--OCH.sub.3) refers to an O-methoxy-ethyl modification of the 2' position of a furanosyl ring. A 2'-O-methoxyethyl modified sugar is a modified sugar.

[0297] "2'-O-methoxyethyl nucleotide" means a nucleotide comprising a 2'-O-methoxyethyl modified sugar moiety.

[0298] "5-methylcytosine" means a cytosine modified with a methyl group attached to position 5. A 5-methylcytosine is a modified nucleobase.

[0299] "About" means within .+-.7% of a value. For example, if it is stated, "the compound affected at least about 70% inhibition of DMPK", it is implied that the DMPK levels are inhibited within a range of 63% and 77%.

[0300] "Active pharmaceutical agent" means the substance or substances in a pharmaceutical composition that provide a therapeutic benefit when administered to an animal. For example, in certain embodiments an antisense oligonucleotide targeted to DMPK is an active pharmaceutical agent.

[0301] "Active target region" or "target region" means a region to which one or more active antisense compounds is targeted. "Active antisense compounds" means antisense compounds that reduce target nucleic acid levels or protein levels.

[0302] "Administered concomitantly" refers to the co-administration of two agents in any manner in which the pharmacological effects of both are manifest in the patient at the same time. Concomitant administration does not require that both agents be administered in a single pharmaceutical composition, in the same dosage form, or by the same route of administration. The effects of both agents need not manifest themselves at the same time. The effects need only be overlapping for a period of time and need not be coextensive.

[0303] "Administering" means providing an agent to an animal, and includes, but is not limited to, administering by a medical professional and self-administering.

[0304] "Agent" means an active substance that can provide a therapeutic benefit when administered to an animal. "First Agent" means a therapeutic compound of the invention. For example, a first agent can be an antisense oligonucleotide targeting DMPK. "Second agent" means a second therapeutic compound of the invention (e.g. a second antisense oligonucleotide targeting DMPK) and/or a non-DMPK therapeutic compound.

[0305] "Amelioration" refers to a lessening of at least one indicator, sign, or symptom of an associated disease, disorder, or condition. The severity of indicators can be determined by subjective or objective measures, which are known to those skilled in the art.

[0306] "Animal" refers to a human or non-human animal, including, but not limited to, mice, rats, rabbits, dogs, cats, pigs, and non-human primates, including, but not limited to, monkeys and chimpanzees.

[0307] "Antisense activity" means any detectable or measurable activity attributable to the hybridization of an antisense compound to its target nucleic acid. In certain embodiments, antisense activity is a decrease in the amount or expression of a target nucleic acid or protein encoded by such target nucleic acid.

[0308] "Antisense compound" means an oligomeric compound that is capable of undergoing hybridization to a target nucleic acid through hydrogen bonding. Examples of antisense compounds include single-stranded and double-stranded compounds, such as, antisense oligonucleotides, siRNAs, shRNAs, snoRNAs, miRNAs, and satellite repeats.

[0309] "Antisense inhibition" means reduction of target nucleic acid levels or target protein levels in the presence of an antisense compound complementary to a target nucleic acid compared to target nucleic acid levels or target protein levels in the absence of the antisense compound.

[0310] "Antisense oligonucleotide" means a single-stranded oligonucleotide having a nucleobase sequence that permits hybridization to a corresponding region or segment of a target nucleic acid.

[0311] "Bicyclic sugar" means a furanosyl ring modified by the bridging of two non-geminal carbon ring atoms. A bicyclic sugar is a modified sugar.

[0312] "Bicyclic nucleic acid" or "BNA" refers to a nucleoside or nucleotide wherein the furanose portion of the nucleoside or nucleotide includes a bridge connecting two carbon atoms on the furanose ring, thereby forming a bicyclic ring system.

[0313] "Cap structure" or "terminal cap moiety" means chemical modifications, which have been incorporated at either terminus of an antisense compound.

[0314] "Chemically distinct region" refers to a region of an antisense compound that is in some way chemically different than another region of the same antisense compound. For example, a region having 2'-O-methoxyethyl nucleotides is chemically distinct from a region having nucleotides without 2'-O-methoxyethyl modifications.

[0315] "Chimeric antisense compound" means an antisense compound that has at least two chemically distinct regions.

[0316] "Co-administration" means administration of two or more agents to an individual. The two or more agents can be in a single pharmaceutical composition, or can be in separate pharmaceutical compositions. Each of the two or more agents can be administered through the same or different routes of administration. Co-administration encompasses parallel or sequential administration.

[0317] "Complementarity" means the capacity for pairing between nucleobases of a first nucleic acid and a second nucleic acid.

[0318] "Contiguous nucleobases" means nucleobases immediately adjacent to each other.

[0319] "CUGexp DMPK" means mutant DMPK RNA containing an expanded CUG repeat (CUGexp). The wild-type DMPK gene has 5-37 CTG repeats in the 3' untranslated region. In a "CUGexp DMPK" (such as in a myotonic dystrophy type I patient) this number is significantly expanded and is, for example, in the range of 50 to greater than 3,500 (Harper, Myotonic Dystrophy (Saunders, London, ed. 3, 2001); Annu. Rev. Neurosci. 29: 259, 2006; EMBO J. 19: 4439, 2000; Curr Opin Neurol. 20: 572, 2007).

[0320] "Diluent" means an ingredient in a composition that lacks pharmacological activity, but is pharmaceutically necessary or desirable. For example, the diluent in an injected composition can be a liquid, e.g. saline solution.

[0321] "DMPK" means any nucleic acid or protein of distrophia myotonica protein kinase. DMPK can be a mutant DMPK including CUGexp DMPK nucleic acid.

[0322] "DMPK expression" means the level of mRNA transcribed from the gene encoding DMPK or the level of protein translated from the mRNA. DMPK expression can be determined by art known methods such as a Northern or Western blot.

[0323] "DMPK nucleic acid" means any nucleic acid encoding DMPK. For example, in certain embodiments, a DMPK nucleic acid includes a DNA sequence encoding DMPK, an RNA sequence transcribed from DNA encoding DMPK (including genomic DNA comprising introns and exons), and an mRNA or pre-mRNA sequence encoding DMPK. "DMPK mRNA" means an mRNA encoding a DMPK protein.

[0324] "Dose" means a specified quantity of a pharmaceutical agent provided in a single administration, or in a specified time period. In certain embodiments, a dose can be administered in one, two, or more boluses, tablets, or injections. For example, in certain embodiments where subcutaneous administration is desired, the desired dose requires a volume not easily accommodated by a single injection, therefore, two or more injections can be used to achieve the desired dose. In certain embodiments, the pharmaceutical agent is administered by infusion over an extended period of time or continuously. Doses can be stated as the amount of pharmaceutical agent per hour, day, week, or month.

[0325] "Effective amount" or "therapeutically effective amount" means the amount of active pharmaceutical agent sufficient to effectuate a desired physiological outcome in an individual in need of the agent. The effective amount can vary among individuals depending on the health and physical condition of the individual to be treated, the taxonomic group of the individuals to be treated, the formulation of the composition, assessment of the individual's medical condition, and other relevant factors.

[0326] "Fully complementary" or "100% complementary" means each nucleobase of a nucleobase sequence of a first nucleic acid has a complementary nucleobase in a second nucleobase sequence of a second nucleic acid. In certain embodiments, a first nucleic acid is an antisense compound and a target nucleic acid is a second nucleic acid.

[0327] "Gapmer" means a chimeric antisense compound in which an internal region having a plurality of nucleosides that support RNase H cleavage is positioned between external regions having one or more nucleosides, wherein the nucleosides comprising the internal region are chemically distinct from the nucleoside or nucleosides comprising the external regions. The internal region can be referred to as a "gap segment" and the external regions can be referred to as "wing segments."

[0328] "Gap-widened" means a chimeric antisense compound having a gap segment of 12 or more contiguous 2'-deoxyribonucleosides positioned between and immediately adjacent to 5' and 3' wing segments having from one to six nucleosides.

[0329] "Hybridization" means the annealing of complementary nucleic acid molecules. In certain embodiments, complementary nucleic acid molecules include an antisense compound and a target nucleic acid.

[0330] "Identifying an animal with type 1 myotonic dystrophy" means identifying an animal having been diagnosed with a type 1 myotonic dystrophy, disorder or condition or identifying an animal predisposed to develop a type 1 myotonic dystrophy, disorder or condition. For example, individuals with a familial history can be predisposed to type 1 myotonic dystrophy, disorder or condition. Such identification can be accomplished by any method including evaluating an individual's medical history and standard clinical tests or assessments.

[0331] "Immediately adjacent" means there are no intervening elements between the immediately adjacent elements.

[0332] "Individual" means a human or non-human animal selected for treatment or therapy.

[0333] "Internucleoside linkage" refers to the chemical bond between nucleosides.

[0334] "Linked nucleosides" means adjacent nucleosides which are bonded or linked together by an internucleoside linkage.

[0335] "Mismatch" or "non-complementary nucleobase" refers to the case when a nucleobase of a first nucleic acid is not capable of pairing with the corresponding nucleobase of a second or target nucleic acid.

[0336] "Modified internucleoside linkage" refers to a substitution or any change from a naturally occurring internucleoside bond (i.e. a phosphodiester internucleoside bond).

[0337] "Modified nucleobase" refers to any nucleobase other than adenine, cytosine, guanine, thymidine, or uracil. An "unmodified nucleobase" means the purine bases adenine (A) and guanine (G), and the pyrimidine bases thymine (T), cytosine (C), and uracil (U).

[0338] "Modified nucleotide" means a nucleotide having, independently, a modified sugar moiety, modified internucleoside linkage, or modified nucleobase. A "modified nucleoside" means a nucleoside having, independently, a modified sugar moiety or modified nucleobase.

[0339] "Modified oligonucleotide" means an oligonucleotide comprising at least one modified nucleoside and/or modified internucleoside linkage.

[0340] "Modified sugar" refers to a substitution or change from a natural sugar moiety. Modified sugars include substituted sugar moieities and surrogate sugar moieties.

[0341] "Motif" means the pattern of chemically distinct regions in an antisense compound.

[0342] "Myotonia" means an abnormally slow relaxation of a muscle after voluntary contraction or electrical stimulation.

[0343] "Nuclear ribonuclease" means a ribonuclease found in the nucleus. Nuclear ribonucleases include, but are not limited to, RNase H including RNase H1 and RNase H2, the double stranded RNase drosha and other double stranded RNases.

[0344] "Naturally occurring internucleoside linkage" means a 3' to 5' phosphodiester linkage.

[0345] "Natural sugar moiety" means a sugar found in DNA (2'-H) or RNA (2'-OH).

[0346] "Nucleic acid" refers to molecules composed of monomeric nucleotides. A nucleic acid includes ribonucleic acids (RNA), deoxyribonucleic acids (DNA), single-stranded nucleic acids, double-stranded nucleic acids, small interfering ribonucleic acids (siRNA), and microRNAs (miRNA). A nucleic acid can also comprise a combination of these elements in a single molecule.

[0347] "Nucleobase" means a heterocyclic moiety capable of pairing with a base of another nucleic acid.

[0348] "Nucleobase sequence" means the order of contiguous nucleobases independent of any sugar, linkage, or nucleobase modification.

[0349] "Nucleoside" means a nucleobase linked to a sugar. In certain embodiments, a nucleoside is linked to a phosphate group.

[0350] "Nucleoside mimetic" includes those structures used to replace the sugar or the sugar and the base and not necessarily the linkage at one or more positions of an oligomeric compound such as for example nucleoside mimetics having morpholino, cyclohexenyl, cyclohexyl, tetrahydropyranyl, bicyclo or tricyclo sugar mimetics e.g. non furanose sugar units.

[0351] "Nucleotide" means a nucleoside having a phosphate group covalently linked to the sugar portion of the nucleoside.

[0352] "Nucleotide mimetic" includes those structures used to replace the nucleoside and the linkage at one or more positions of an oligomeric compound such as for example peptide nucleic acids or morpholinos (morpholinos linked by --N(H)--C(.dbd.O)--O-- or other non-phosphodiester linkage).

[0353] "Oligomeric compound" or "oligomer" means a polymer of linked monomeric subunits which is capable of hybridizing to at least a region of a nucleic acid molecule.

[0354] "Oligonucleotide" means a polymer of linked nucleosides, wherein each nucleoside and each internucleoside linkage may be modified or unmodified, independent one from another.

[0355] "Parenteral administration" means administration through injection or infusion. Parenteral administration includes subcutaneous administration, intravenous administration, intramuscular administration, intraarterial administration, intraperitoneal administration, or intracranial administration, e.g. intrathecal or intracerebroventricular administration. Administration can be continuous, or chronic, or short or intermittent.

[0356] "Peptide" means a molecule formed by linking at least two amino acids by amide bonds. Peptide refers to polypeptides and proteins.

[0357] "Pharmaceutical composition" means a mixture of substances suitable for administering to an individual. For example, a pharmaceutical composition can comprise one or more active agents and a sterile aqueous solution.

[0358] "Pharmaceutically acceptable salts" means physiologically and pharmaceutically acceptable salts of antisense compounds, i.e., salts that retain the desired biological activity of the parent oligonucleotide and do not impart undesired toxicological effects thereto.

[0359] "Phosphorothioate linkage" means a linkage between nucleosides where the phosphodiester bond is modified by replacing one of the non-bridging oxygen atoms with a sulfur atom. A phosphorothioate linkage is a modified internucleoside linkage.

[0360] "Portion" means a defined number of contiguous (i.e. linked) nucleobases of a nucleic acid.

[0361] In certain embodiments, a portion is a defined number of contiguous nucleobases of a target nucleic acid. In certain embodiments, a portion is a defined number of contiguous nucleobases of an antisense compound.

[0362] "Preferentially reducing CUG exp DMPK RNA" refers to a preferential reduction of RNA transcripts from a CUGexp DMPK allele relative to RNA transcripts from a normal DMPK allele.

[0363] "Prevent" refers to delaying or forestalling the onset or development of a disease, disorder, or condition for a period of time from minutes to indefinitely. Prevent also means reducing risk of developing a disease, disorder, or condition.

[0364] "Prodrug" means a therapeutic agent that is prepared in an inactive form that is converted to an active form within the body or cells thereof by the action of endogenous enzymes or other chemicals or conditions.

[0365] "Side effects" means physiological responses attributable to a treatment other than the desired effects. In certain embodiments, side effects include injection site reactions, liver function test abnormalities, renal function abnormalities, liver toxicity, renal toxicity, central nervous system abnormalities, myopathies, and malaise. For example, increased aminotransferase levels in serum can indicate liver toxicity or liver function abnormality. For example, increased bilirubin can indicate liver toxicity or liver function abnormality.

[0366] "Single-stranded oligonucleotide" means an oligonucleotide which is not hybridized to a complementary strand.

[0367] "Specifically hybridizable" refers to an antisense compound having a sufficient degree of complementarity between an antisense oligonucleotide and a target nucleic acid to induce a desired effect, while exhibiting minimal or no effects on non-target nucleic acids under conditions in which specific binding is desired, i.e. under physiological conditions in the case of in vivo assays and therapeutic treatments.

[0368] "Spliceopathy" means a change in the alternative splicing of one or more RNAs that leads to the expression of altered splice products in a particular tissue.

[0369] "Subcutaneous administration" means administration just below the skin.

[0370] "Substituted sugar moiety" means a furanosyl other than a natural sugar of RNA or DNA.

[0371] "Sugar" or "Sugar moiety" means a natural sugar moiety or a modified sugar.

[0372] "Sugar surrogate" overlaps with the slightly broader term "nucleoside mimetic" but is intended to indicate replacement of the sugar unit (furanose ring) only A sugar surrogate is capable of replacing the naturally occurring sugar moiety of a nucleoside, such that the resulting nucleoside sub-units are capable of linking together and/or linking to other nucleosides to form an oligomeric compound which is capable of hybridizing to a complementary oligomeric compound. Such structures include rings comprising a different number of atoms than furanosyl (e.g., 4, 6, or 7-membered rings); replacement of the oxygen of a furanosyl with a non-oxygen atom (e.g., carbon, sulfur, or nitrogen); or both a change in the number of atoms and a replacement of the oxygen. Such structures may also comprise substitutions corresponding to those described for substituted sugar moieties (e.g., 6-membered carbocyclic bicyclic sugar surrogates optionally comprising additional substituents). Sugar surrogates also include more complex sugar replacements (e.g., the non-ring systems of peptide nucleic acid). Sugar surrogates include without limitation morpholinos, cyclohexenyls and cyclohexitols.

[0373] "Targeting" or "targeted" means the process of design and selection of an antisense compound that will specifically hybridize to a target nucleic acid and induce a desired effect.

[0374] "Target nucleic acid," "target RNA," and "target RNA transcript" all refer to a nucleic acid capable of being targeted by antisense compounds. In certain embodiments, a target nucleic acid comprises a region of a DMPK nucleic acid.

[0375] "Target segment" means the sequence of nucleotides of a target nucleic acid to which an antisense compound is targeted. "5' target site" refers to the 5'-most nucleotide of a target segment. "3' target site" refers to the 3'-most nucleotide of a target segment.

[0376] "Therapeutically effective amount" means an amount of an agent that provides a therapeutic benefit to an individual.

[0377] "Treat" refers to administering a pharmaceutical composition to effect an alteration or improvement of a disease, disorder, or condition.

[0378] "Type 1 myotonic dystrophy" or "DM1" means an autosomal dominant disorder caused by expansion of a non-coding CTG repeat in DMPK. This mutation leads to RNA dominance, a process in which expression of RNA containing an expanded CUG repeat (CUGexp) induced cell dysfunction. The CUGexp tract interacts with RNA binding proteins and causes the mutant transcript to be retained in nuclear foci. The toxicity of this RNA stems from sequestration of RNA binding proteins and activation of signaling pathways.

[0379] "Unmodified nucleotide" means a nucleotide composed of naturally occurring nucleobases, sugar moieties, and internucleoside linkages. In certain embodiments, an unmodified nucleotide is an RNA nucleotide (i.e. .beta.-D-ribonucleosides) or a DNA nucleotide (i.e. .beta.-D-deoxyribonucleoside).

Certain Embodiments

[0380] Certain embodiments provide methods, compounds, and compositions for inhibiting DMPK expression.

[0381] Certain embodiments provide a method of reducing DMPK expression in an animal comprising administering to the animal a compound comprising a modified oligonucleotide targeting DMPK.

[0382] Certain embodiments provide a method of preferentially reducing CUGexp DMPK RNA, reducing myotonia or reducing spliceopathy in an animal comprising administering to the animal a compound comprising a modified oligonucleotide targeted to DMPK, wherein the modified oligonucleotide preferentially reduces CUGexp DMPK RNA, reduces myotonia or reduces spliceopathy in the animal.

[0383] Certain embodiments provide a method of administering an antisense oligonucleotide to counteract RNA dominance by directing the cleavage of pathogenic transcripts.

[0384] Certain embodiments provide a method of reducing spliceopathy of Serca1. In certain embodiments, methods provided herein result in exon 22 inclusion. In certain embodiments, the corrective splicing occurs in the tibialis anterior, gastrocnemius, and quadriceps muscles.

[0385] Certain embodiments provide a method of reducing spliceopathy of m-Titin. In certain embodiments, methods provided herein result in exon 5 inclusion. In certain embodiments, the corrective splicing occurs in the tibialis anterior, gastrocnemius, and quadriceps muscles.

[0386] Certain embodiments provide a method of reducing spliceopathy of Clcn1. In certain embodiments, methods provided herein result in exon 7a inclusion. In certain embodiments, the corrective splicing occurs in the tibialis anterior, gastrocnemius, and quadriceps muscles.

[0387] Certain embodiments provide a method of reducing spliceopathy of Zasp. In certain embodiments, methods provided herein result in exon 11 inclusion. In certain embodiments, the corrective splicing occurs in the tibialis anterior, gastrocnemius, and quadriceps muscles.

[0388] Certain embodiments provide a method for treating an animal with type 1 myotonic dystrophy comprising: a) identifying said animal with type 1 myotonic dystrophy, and b) administering to said animal a therapeutically effective amount of a compound comprising a modified oligonucleotide targeted to DMPK. In certain embodiments, the therapeutically effective amount of the compound administered to the animal preferentially reduces CUGexp DMPK RNA, reduces myotonia or reduces spliceopathy in the animal.

[0389] Certain embodiments provide a method of achieving a preferential reduction of CUGexp DMPK RNA, including administering to the subject suspected of having type 1 myotonic dystrophy or having a CUGexp DMPK RNA a modified antisense oligonucleotide complementary to a non-repeat region of said CUGexp DMPK RNA. The modified antisense oligonucleotide, when bound to said CUGexp DMPK RNA, achieves a preferential reduction of the CUGexp DMPK RNA.

[0390] Certain embodiments provide a method of achieving a preferential reduction of CUGexp DMPK RNA, including selecting a subject having type 1 myotonic dystrophy or having a CUGexp DMPK RNA and administering to said subject a modified antisense oligonucleotide complementary to a non-repeat region of said CUGexp DMPK RNA. The modified antisense oligonucleotide, when bound to the CUGexp DMPK RNA, activates a ribonuclease or nuclear ribonuclease, thereby achieving a preferential reduction of the CUGexp DMPK RNA in the nucleus.

[0391] Certain embodiments provide a method of achieving a preferential reduction of CUGexp DMPK RNA, including selecting a subject having type 1 myotonic dystrophy or having a mutant or CUGexp DMPK RNA and systemically administering to said subject a modified antisense oligonucleotide complementary to a non-repeat region of said CUGexp DMPK RNA. The modified antisense oligonucleotide, when bound to the mutant or CUGexp DMPK RNA, achieves a preferential reduction of the mutant or CUGexp DMPK RNA.

[0392] Certain embodiments provide a method of reducing myotonia in a subject in need thereof. The method includes administering to the subject a modified antisense oligonucleotide complementary to a non-repeat region of a DMPK RNA, wherein the modified antisense oligonucleotide, when bound to the DMPK RNA, activates a ribonuclease or nuclear ribonuclease, thereby reducing myotonia. In certain embodiments, the subject has or is suspected of having type 1 myotonic dystrophy or having a mutant DMPK RNA or CUGexp DMPK RNA. In certain embodiments, the DMPK RNA is nuclear retained.

[0393] Certain embodiments provide a method of reducing spliceopathy in a subject in need thereof. The method includes administering to the subject a modified antisense oligonucleotide complementary to a non-repeat region of a DMPK RNA, wherein the modified antisense oligonucleotide, when bound to the DMPK RNA, activates a ribonuclease or nuclear ribonuclease, thereby reducing spliceopathy. In certain embodiments, the subject has or is suspected of having type 1 myotonic dystrophy or having a nuclear retained CUGexp DMPK RNA. In certain embodiments, the DMPK RNA is nuclear retained. In certain embodiments, the spliceopathy is MBNL dependent spliceopathy.

[0394] In certain embodiments, the modified antisense oligonucleotide of the methods is chimeric. In certain embodiments, the modified antisense oligonucleotide of the methods is a gapmer.

[0395] In certain embodiments of the methods provided herein, the administering is subcutaneous. In certain embodiments, the administering is intravenous.

[0396] In certain embodiments, the modified antisense oligonucleotide of the methods targets a non-coding sequence within the non-repeat region of a DMPK RNA. In certain embodiments, the oligonucleotide targets a coding region, an intron, a 5'UTR, or a 3'UTR of the mutant DMPK RNA.

[0397] In certain embodiments of the methods provided herein, the nuclear ribonuclease is RNase H1.

[0398] In certain embodiments of the methods, the DMPK RNA is reduced in muscle tissue. In certain embodiments, the mutant DMPK RNA CUGexp DMPK RNA is preferentially reduced.

[0399] In certain embodiments, the DMPK has the sequence as set forth in GenBank Accession No. NM_001081560.1 (incorporated herein as SEQ ID NO: 1). In certain embodiments, the DMPK has the sequence as set forth in GenBank Accession No. NT_011109.15 truncated from nucleotides 18540696 to Ser. No. 18/555,106 (incorporated herein as SEQ ID NO: 2). In certain embodiments, the DMPK has the sequence as set forth in GenBank Accession No. NT_039413.7 truncated from nucleotides 16666001 to Ser. No. 16/681,000 (incorporated herein as SEQ ID NO: 3). In certain embodiments, the DMPK has the sequence as set forth in GenBank Accession No. NM_032418.1 (incorporated herein as SEQ ID NO: 4). In certain embodiments, the DMPK has the sequence as set forth in GenBank Accession No. AI007148.1 (incorporated herein as SEQ ID NO: 5). In certain embodiments, the DMPK has the sequence as set forth in GenBank Accession No. AI304033.1 (incorporated herein as SEQ ID NO: 6). In certain embodiments, the DMPK has the sequence as set forth in GenBank Accession No. BC024150.1 (incorporated herein as SEQ ID NO: 7). In certain embodiments, the DMPK has the sequence as set forth in GenBank Accession No. BC056615.1 (incorporated herein as SEQ ID NO: 8). In certain embodiments, the DMPK has the sequence as set forth in GenBank Accession No. BC075715.1 (incorporated herein as SEQ ID NO: 9). In certain embodiments, the DMPK has the sequence as set forth in GenBank Accession No. BU519245.1 (incorporated herein as SEQ ID NO: 10). In certain embodiments, the DMPK has the sequence as set forth in GenBank Accession No. CB247909.1 (incorporated herein as SEQ ID NO: 11). In certain embodiments, the DMPK has the sequence as set forth in GenBank Accession No. CX208906.1 (incorporated herein as SEQ ID NO: 12). In certain embodiments, the DMPK has the sequence as set forth in GenBank Accession No. CX732022.1 (incorporated herein as SEQ ID NO: 13). In certain embodiments, the DMPK has the sequence as set forth in GenBank Accession No. S60315.1 (incorporated herein as SEQ ID NO: 14). In certain embodiments, the DMPK has the sequence as set forth in GenBank Accession No. S60316.1 (incorporated herein as SEQ ID NO: 15). In certain embodiments, the DMPK has the sequence as set forth in GenBank Accession No. NM_001081562.1 (incorporated herein as SEQ ID NO: 16). In certain embodiments, the DMPK has the sequence as set forth in GenBank Accession No. NM_001100.3 (incorporated herein as SEQ ID NO: 17).

[0400] In certain embodiments, the modified oligonucleotide has a nucleobase sequence comprising at least 8 contiguous nucleobases of a nucleobase sequence recited in any one of SEQ ID NOs: 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, or 33-874. In certain embodiments, the modified oligonucleotide has a nucleobase sequence comprising at least 9, at least 10, or at least 11, contiguous nucleobases of a nucleobase sequence recited in any one of SEQ ID NOs: 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, or 33-874.

[0401] In certain embodiments, the modified oligonucleotide has a nucleobase sequence comprising at least 12 contiguous nucleobases of a nucleobase sequence recited in any one of SEQ ID NOs: 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, or 33-874. In certain embodiments, the modified oligonucleotide has a nucleobase sequence comprising at least 13, or at least 14, contiguous nucleobases of a nucleobase sequence recited in any one of SEQ ID NOs: 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, or 33-874.

[0402] In certain embodiments, the modified oligonucleotide has a nucleobase sequence comprising at least 15 contiguous nucleobases of a nucleobase sequence recited in any one of SEQ ID NOs: 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, or 33-874. In certain embodiments, the modified oligonucleotide has a nucleobase sequence comprising at least 16 contiguous nucleobases of a nucleobase sequence recited in any one of SEQ ID NOs: 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, or 33-874.

[0403] In certain embodiments, the modified oligonucleotide has a nucleobase sequence comprising at least 17 contiguous nucleobases of a nucleobase sequence recited in any one of SEQ ID NOs: 24, 25, 27, or 28.

[0404] In certain embodiments, the modified oligonucleotide has a nucleobase sequence comprising at least 18 contiguous nucleobases of a nucleobase sequence recited in any one of SEQ ID NOs: 24 or 25. In certain embodiments, the modified oligonucleotide has a nucleobase sequence comprising at least 19 contiguous nucleobases of a nucleobase sequence recited in any one of SEQ ID NOs: 24 or 25.

[0405] In certain embodiments, the modified oligonucleotides provided herein are targeted to any one of the following regions of SEQ ID NO: 1: 1343-1368, 1317-1366, 2748-2791, 2155-2208, 2748-2791, 730-748, 528-547, 531-567, 636-697, 1311-1331, 1314-1339, 1446-1475, 1635-1670, 1610-1638, 1457-1486, 2773-1788, 931-948, 934-949, 937-952, 942-957, 937-957, 943-958, 937-953, 1346-1363, 1346-1361, 1347-1363, 2162-2179, 2492-2508, 2696-2717, and 2683-2703. In certain embodiments, the modified oligonucleotides provided herein are targeted to any one of the following regions of SEQ ID NO: 1: 2773-2788, 1343-1358, and 1344-1359.

[0406] In certain embodiments, the modified oligonucleotides provided herein have a nucleobase sequence comprising a complementary region comprising at least 8 contiguous nucleobases complementary to a target region. In certain embodiments, the modified oligonucleotides provided herein have a nucleobase sequence comprising a complementary region comprising at least 8 contiguous nucleobases complementary to a target region, wherein the target region is targeted to nucleobases 1343-1368, 1317-1366, 2748-2791, 2155-2208, 2748-2791, 730-748, 528-547, 531-567, 636-697, 1311-1331, 1314-1339, 1446-1475, 1635-1670, 1610-1638, 1457-1486, 2773-1788, 931-948, 934-949, 937-952, 942-957, 937-957, 943-958, 937-953, 1346-1363, 1346-1361, 1347-1363, 2162-2179, 2492-2508, 2696-2717, or 2683-2703 of SEQ ID NO: 1. In certain embodiments, the modified oligonucleotides provided herein have a nucleobase sequence comprising a complementary region comprising at least 8 contiguous nucleobases complementary to a target region, wherein the target region is targeted to nucleobases 2773-2788, 1343-1358, or 1344-1359 of SEQ ID NO: 1.

[0407] In certain embodiments, the modified oligonucleotides provided herein have a nucleobase sequence comprising a complementary region comprising at least 10 contiguous nucleobases complementary to a target region. In certain embodiments, the modified oligonucleotides provided herein have a nucleobase sequence comprising a complementary region comprising at least 10 contiguous nucleobases complementary to a target region, wherein the target region is targeted to nucleobases 1343-1368, 1317-1366, 2748-2791, 2155-2208, 2748-2791, 730-748, 528-547, 531-567, 636-697, 1311-1331, 1314-1339, 1446-1475, 1635-1670, 1610-1638, 1457-1486, 2773-1788, 931-948, 934-949, 937-952, 942-957, 937-957, 943-958, 937-953, 1346-1363, 1346-1361, 1347-1363, 2162-2179, 2492-2508, 2696-2717, or 2683-2703 of SEQ ID NO: 1. In certain embodiments, the modified oligonucleotides provided herein have a nucleobase sequence comprising a complementary region comprising at least 10 contiguous nucleobases complementary to a target region, wherein the target region is targeted to nucleobases 2773-2788, 1343-1358, or 1344-1359 of SEQ ID NO: 1.

[0408] In certain embodiments, the modified oligonucleotides provided herein have a nucleobase sequence comprising a complementary region comprising at least 12 contiguous nucleobases complementary to a target region. In certain embodiments, the modified oligonucleotides provided herein have a nucleobase sequence comprising a complementary region comprising at least 12 contiguous nucleobases complementary to a target region, wherein the target region is targeted to nucleobases 1343-1368, 1317-1366, 2748-2791, 2155-2208, 2748-2791, 730-748, 528-547, 531-567, 636-697, 1311-1331, 1314-1339, 1446-1475, 1635-1670, 1610-1638, 1457-1486, 2773-1788, 931-948, 934-949, 937-952, 942-957, 937-957, 943-958, 937-953, 1346-1363, 1346-1361, 1347-1363, 2162-2179, 2492-2508, 2696-2717, or 2683-2703 of SEQ ID NO: 1. In certain embodiments, the modified oligonucleotides provided herein have a nucleobase sequence comprising a complementary region comprising at least 12 contiguous nucleobases complementary to a target region, wherein the target region is targeted to nucleobases 2773-2788, 1343-1358, or 1344-1359 of SEQ ID NO: 1.

[0409] In certain embodiments, the modified oligonucleotides provided herein have a nucleobase sequence comprising a complementary region comprising at least 14 contiguous nucleobases complementary to a target region. In certain embodiments, the modified oligonucleotides provided herein have a nucleobase sequence comprising a complementary region comprising at least 14 contiguous nucleobases complementary to a target region, wherein the target region is targeted to nucleobases 1343-1368, 1317-1366, 2748-2791, 2155-2208, 2748-2791, 730-748, 528-547, 531-567, 636-697, 1311-1331, 1314-1339, 1446-1475, 1635-1670, 1610-1638, 1457-1486, 2773-1788, 931-948, 934-949, 937-952, 942-957, 937-957, 943-958, 937-953, 1346-1363, 1346-1361, 1347-1363, 2162-2179, 2492-2508, 2696-2717, or 2683-2703 of SEQ ID NO: 1. In certain embodiments, the modified oligonucleotides provided herein have a nucleobase sequence comprising a complementary region comprising at least 14 contiguous nucleobases complementary to a target region, wherein the target region is targeted to nucleobases 2773-2788, 1343-1358, or 1344-1359 of SEQ ID NO: 1.

[0410] In certain embodiments, the modified oligonucleotides provided herein have a nucleobase sequence comprising a complementary region comprising at least 16 contiguous nucleobases complementary to a target region. In certain embodiments, the modified oligonucleotides provided herein have a nucleobase sequence comprising a complementary region comprising at least 16 contiguous nucleobases complementary to a target region, wherein the target region is targeted to nucleobases 1343-1368, 1317-1366, 2748-2791, 2155-2208, 2748-2791, 730-748, 528-547, 531-567, 636-697, 1311-1331, 1314-1339, 1446-1475, 1635-1670, 1610-1638, 1457-1486, 2773-1788, 931-948, 934-949, 937-952, 942-957, 937-957, 943-958, 937-953, 1346-1363, 1346-1361, 1347-1363, 2162-2179, 2492-2508, 2696-2717, or 2683-2703 of SEQ ID NO: 1. In certain embodiments, the modified oligonucleotides provided herein have a nucleobase sequence comprising a complementary region comprising at least 16 contiguous nucleobases complementary to a target region, wherein the target region is targeted to nucleobases 2773-2788, 1343-1358, or 1344-1359 of SEQ ID NO: 1.

[0411] In certain embodiments, the modified oligonucleotides provided herein are targeted to any one of the following regions of SEQ ID NO: 2: 10195-10294, 13553-13572, 13748-13767, 13455-13475, 13628-13657, 13735-13760, 13746-13905, 13836-13851, 13553-13568, 13563-13578, 13624-13639, 13686-13701, 13760-13775, 13763-13779, 13765-13780, 2580-2595, 6446-6461, 11099-11115, 11082-11099, 1974-1993, 4435-4456, 6035-6052, 6360-6385, 6445-6468, 6807-6824, 6789-6806, and 6596-6615. In certain embodiments, the modified oligonucleotides provided herein are targeted to any one of the following regions of SEQ ID NO: 2: 13836-13831, 8603-8618, and 8604-8619.

[0412] In certain embodiments, the modified oligonucleotides provided herein have a nucleobase sequence comprising a complementary region comprising at least 8 contiguous nucleobases complementary to a target region. In certain embodiments, the modified oligonucleotides provided herein have a nucleobase sequence comprising a complementary region comprising at least 8 contiguous nucleobases complementary to a target region, wherein the target region is targeted to nucleobases 10195-10294, 13553-13572, 13748-13767, 13455-13475, 13628-13657, 13735-13760, 13746-13905, 13836-13851, 13553-13568, 13563-13578, 13624-13639, 13686-13701, 13760-13775, 13763-13779, 13765-13780, 2580-2595, 6446-6461, 11099-11115, 11082-11099, 1974-1993, 4435-4456, 6035-6052, 6360-6385, 6445-6468, 6807-6824, 6789-6806, or 6596-6615 of SEQ ID NO: 2. In certain embodiments, the modified oligonucleotides provided herein have a nucleobase sequence comprising a complementary region comprising at least 8 contiguous nucleobases complementary to a target region, wherein the target region is targeted to nucleobases 13836-13831, 8603-8618, or 8604-8619 of SEQ ID NO: 2.

[0413] In certain embodiments, the modified oligonucleotides provided herein have a nucleobase sequence comprising a complementary region comprising at least 10 contiguous nucleobases complementary to a target region. In certain embodiments, the modified oligonucleotides provided herein have a nucleobase sequence comprising a complementary region comprising at least 10 contiguous nucleobases complementary to a target region, wherein the target region is targeted to nucleobases 10195-10294, 13553-13572, 13748-13767, 13455-13475, 13628-13657, 13735-13760, 13746-13905, 13836-13851, 13553-13568, 13563-13578, 13624-13639, 13686-13701, 13760-13775, 13763-13779, 13765-13780, 2580-2595, 6446-6461, 11099-11115, 11082-11099, 1974-1993, 4435-4456, 6035-6052, 6360-6385, 6445-6468, 6807-6824, 6789-6806, or 6596-6615 of SEQ ID NO: 2. In certain embodiments, the modified oligonucleotides provided herein have a nucleobase sequence comprising a complementary region comprising at least 10 contiguous nucleobases complementary to a target region, wherein the target region is targeted to nucleobases 13836-13831, 8603-8618, or 8604-8619 of SEQ ID NO: 2.

[0414] In certain embodiments, the modified oligonucleotides provided herein have a nucleobase sequence comprising a complementary region comprising at least 12 contiguous nucleobases complementary to a target region. In certain embodiments, the modified oligonucleotides provided herein have a nucleobase sequence comprising a complementary region comprising at least 12 contiguous nucleobases complementary to a target region, wherein the target region is targeted to nucleobases 10195-10294, 13553-13572, 13748-13767, 13455-13475, 13628-13657, 13735-13760, 13746-13905, 13836-13851, 13553-13568, 13563-13578, 13624-13639, 13686-13701, 13760-13775, 13763-13779, 13765-13780, 2580-2595, 6446-6461, 11099-11115, 11082-11099, 1974-1993, 4435-4456, 6035-6052, 6360-6385, 6445-6468, 6807-6824, 6789-6806, or 6596-6615 of SEQ ID NO: 2. In certain embodiments, the modified oligonucleotides provided herein have a nucleobase sequence comprising a complementary region comprising at least 12 contiguous nucleobases complementary to a target region, wherein the target region is targeted to nucleobases 13836-13831, 8603-8618, or 8604-8619 of SEQ ID NO: 2.

[0415] In certain embodiments, the modified oligonucleotides provided herein have a nucleobase sequence comprising a complementary region comprising at least 14 contiguous nucleobases complementary to a target region. In certain embodiments, the modified oligonucleotides provided herein have a nucleobase sequence comprising a complementary region comprising at least 14 contiguous nucleobases complementary to a target region, wherein the target region is targeted to nucleobases 10195-10294, 13553-13572, 13748-13767, 13455-13475, 13628-13657, 13735-13760, 13746-13905, 13836-13851, 13553-13568, 13563-13578, 13624-13639, 13686-13701, 13760-13775, 13763-13779, 13765-13780, 2580-2595, 6446-6461, 11099-11115, 11082-11099, 1974-1993, 4435-4456, 6035-6052, 6360-6385, 6445-6468, 6807-6824, 6789-6806, or 6596-6615 of SEQ ID NO: 2. In certain embodiments, the modified oligonucleotides provided herein have a nucleobase sequence comprising a complementary region comprising at least 14 contiguous nucleobases complementary to a target region, wherein the target region is targeted to nucleobases 13836-13831, 8603-8618, or 8604-8619 of SEQ ID NO: 2.

[0416] In certain embodiments, the modified oligonucleotides provided herein have a nucleobase sequence comprising a complementary region comprising at least 16 contiguous nucleobases complementary to a target region. In certain embodiments, the modified oligonucleotides provided herein have a nucleobase sequence comprising a complementary region comprising at least 16 contiguous nucleobases complementary to a target region, wherein the target region is targeted to nucleobases 10195-10294, 13553-13572, 13748-13767, 13455-13475, 13628-13657, 13735-13760, 13746-13905, 13836-13851, 13553-13568, 13563-13578, 13624-13639, 13686-13701, 13760-13775, 13763-13779, 13765-13780, 2580-2595, 6446-6461, 11099-11115, 11082-11099, 1974-1993, 4435-4456, 6035-6052, 6360-6385, 6445-6468, 6807-6824, 6789-6806, or 6596-6615 of SEQ ID NO: 2. In certain embodiments, the modified oligonucleotides provided herein have a nucleobase sequence comprising a complementary region comprising at least 16 contiguous nucleobases complementary to a target region, wherein the target region is targeted to nucleobases 13836-13831, 8603-8618, or 8604-8619 of SEQ ID NO: 2.

[0417] In certain embodiments, the animal is a human.

[0418] In certain embodiments, the compounds or compositions of the invention are designated as a first agent and the methods of the invention further comprise administering a second agent. In certain embodiments, the first agent and the second agent are co-administered. In certain embodiments the first agent and the second agent are co-administered sequentially or concomitantly.

[0419] In certain embodiments, administration comprises parenteral administration.

[0420] In certain embodiments, the compound is a single-stranded modified oligonucleotide. In certain embodiments, the nucleobase sequence of the modified oligonucleotide is at least 95% complementary to any one of SEQ ID NOs: 1-19 as measured over the entirety of said modified oligonucleotide. In certain embodiments, the nucleobase sequence of the modified oligonucleotide is 100% complementary to any one of SEQ ID NOs: 1-19 as measured over the entirety of said modified oligonucleotide. In certain embodiments, the compound is a single-stranded modified oligonucleotide. In certain embodiments, the nucleobase sequence of the modified oligonucleotide is at least 95% complementary to any one of SEQ ID NO: 1 as measured over the entirety of said modified oligonucleotide. In certain embodiments, the nucleobase sequence of the modified oligonucleotide is 100% complementary to any one of SEQ ID NO: 1 as measured over the entirety of said modified oligonucleotide.

[0421] In certain embodiments, the nucleobase sequence of the modified oligonucleotide is at least 90% complementary to any one of SEQ ID NO: 1 as measured over the entirety of said modified oligonucleotide. In certain embodiments, the nucleobase sequence of the modified oligonucleotide is 85% complementary to any one of SEQ ID NOs: 1 as measured over the entirety of said modified oligonucleotide.

[0422] In certain embodiments, the nucleobase sequence of the modified oligonucleotide is at least 90% complementary to any one of SEQ ID NO: 2 as measured over the entirety of said modified oligonucleotide. In certain embodiments, the nucleobase sequence of the modified oligonucleotide is 85% complementary to any one of SEQ ID NO: 2 as measured over the entirety of said modified oligonucleotide.

[0423] In certain embodiments, at least one internucleoside linkage of said modified oligonucleotide is a modified internucleoside linkage. In certain embodiments, each internucleoside linkage is a phosphorothioate internucleoside linkage.

[0424] In certain embodiments, at least one nucleoside of said modified oligonucleotide comprises a modified sugar. In certain embodiments, at least one modified sugar is a bicyclic sugar. In certain embodiments, at least one modified sugar comprises a 2'-O-methoxyethyl or a 4'-(CH.sub.2).sub.n--O-2' bridge, wherein n is 1 or 2.

[0425] In certain embodiments, at least one nucleoside of said modified oligonucleotide comprises a modified nucleobase. In certain embodiments, the modified nucleobase is a 5-methylcytosine.

[0426] In certain embodiments, the modified oligonucleotide comprises: a) a gap segment consisting of linked deoxynucleosides; b) a 5' wing segment consisting of linked nucleosides; and c) a 3' wing segment consisting of linked nucleosides. The gap segment is positioned between the 5' wing segment and the 3' wing segment and each nucleoside of each wing segment comprises a modified sugar.

[0427] In certain embodiments, the modified oligonucleotide comprises: a) a gap segment consisting often linked deoxynucleosides; b) a 5' wing segment consisting of five linked nucleosides; and c) a 3' wing segment consisting of five linked nucleosides. The gap segment is positioned between the 5' wing segment and the 3' wing segment, each nucleoside of each wing segment comprises a 2'-O-methoxyethyl sugar, each internucleoside linkage of said modified oligonucleotide is a phosphorothioate linkage, and each cytosine in said modified oligonucleotide is a 5'-methylcytosine.

[0428] In certain embodiments, the modified oligonucleotide consists of 20 linked nucleosides. In certain embodiments, the modified oligonucleotide consists of 19 linked nucleosides. In certain embodiments, the modified oligonucleotide consists of 18 linked nucleosides. In certain embodiments, the modified oligonucleotide consists of 17 linked nucleosides. In certain embodiments, the modified oligonucleotide consists of 16 linked nucleosides.

[0429] Certain embodiments provide a method of preferentially reducing CUGexp DMPK RNA, reducing myotonia or reducing spliceopathy in an animal comprising administering to the animal a compound comprising a modified oligonucleotide having a gap segment consisting often linked deoxynucleosides, a 5' wing segment consisting of five linked nucleosides and a 3' wing segment consisting of five linked nucleosides. The gap segment is positioned between the 5' wing segment and the 3' wing segment, each nucleoside of each wing segment comprises a 2'-O-methoxyethyl sugar, each internucleoside linkage of said modified oligonucleotide is a phosphorothioate linkage, each cytosine in said modified oligonucleotide is a 5'-methylcytosine.

[0430] In certain embodiments, the modified oligonucleotide comprises: a) a gap segment consisting of eight linked deoxynucleosides; b) a 5' wing segment consisting of four linked nucleosides and having a E-E-K-K 5'-wing motif; c) a 3' wing segment consisting of four linked nucleosides and having a K-K-E-E 3'-wing motif; and d) wherein the gap segment is positioned between the 5' wing segment and the 3' wing segment, and wherein each E represents 2'-O-methoxyethyl sugar and each K represents a cEt sugar.

[0431] In certain embodiments, the modified oligonucleotide comprises: a) a gap segment consisting of seven linked deoxynucleosides; b) a 5' wing segment consisting of five linked nucleosides and having an E-E-E-K-K 5'-wing motif; c) a 3' wing segment consisting of five linked nucleosides and having a K-K-E-E-E 3'-wing motif; and d) wherein the gap segment is positioned between the 5' wing segment and the 3' wing segment, and wherein each E represents 2'-O-methoxyethyl sugar and each K represents a cEt sugar.

[0432] In certain embodiments, the modified oligonucleotide comprises: a) a gap segment consisting often linked deoxynucleosides; b) a 5' wing segment consisting of five linked nucleosides; c) a 3' wing segment consisting of five linked nucleosides; and d) wherein the gap segment is positioned between the 5' wing segment and the 3' wing segment, and wherein each nucleoside of each wing segment comprises a 2'-O-methoxyethyl sugar.

[0433] In certain embodiments, the modified oligonucleotide comprises: a) a gap segment consisting often linked deoxynucleosides; b) a 5' wing segment consisting of three linked nucleosides; c) a 3' wing segment consisting of three linked nucleosides; and d) wherein the gap segment is positioned between the 5' wing segment and the 3' wing segment, and wherein each nucleoside of each wing segment comprises a cEt sugar.

[0434] Certain embodiments provide a method of preferentially reducing CUGexp DMPK RNA, reducing myotonia or reducing spliceopathy in an animal comprising administering to the animal a compound comprising a modified oligonucleotide having: a) a gap segment consisting of eight linked deoxynucleosides; b) a 5' wing segment consisting of four linked nucleosides and having a E-E-K-K 5'-wing motif; c) a 3' wing segment consisting of four linked nucleosides and having a K-K-E-E 3'-wing motif; and d) wherein the gap segment is positioned between the 5' wing segment and the 3' wing segment, and wherein each E represents 2'-O-methoxyethyl sugar and each K represents a cEt sugar.

[0435] Certain embodiments provide a method of preferentially reducing CUGexp DMPK RNA, reducing myotonia or reducing spliceopathy in an animal comprising administering to the animal a compound comprising a modified oligonucleotide having: a) a gap segment consisting of seven linked deoxynucleosides; b) a 5' wing segment consisting of five linked nucleosides and having an E-E-E-K-K 5'-wing motif; c) a 3' wing segment consisting of five linked nucleosides and having a K-K-E-E-E 3'-wing motif; and d) wherein the gap segment is positioned between the 5' wing segment and the 3' wing segment, and wherein each E represents 2'-O-methoxyethyl sugar and each K represents a cEt sugar.

[0436] Certain embodiments provide a method of preferentially reducing CUGexp DMPK RNA, reducing myotonia or reducing spliceopathy in an animal comprising administering to the animal a compound comprising a modified oligonucleotide having: a) a gap segment consisting often linked deoxynucleosides; b) a 5' wing segment consisting of five linked nucleosides; c) a 3' wing segment consisting of five linked nucleosides; and d) wherein the gap segment is positioned between the 5' wing segment and the 3' wing segment, and wherein each nucleoside of each wing segment comprises a 2'-O-methoxyethyl sugar.

[0437] Certain embodiments provide a method of preferentially reducing CUGexp DMPK RNA, reducing myotonia or reducing spliceopathy in an animal comprising administering to the animal a compound comprising a modified oligonucleotide having: a) a gap segment consisting of ten linked deoxynucleosides; b) a 5' wing segment consisting of three linked nucleosides; c) a 3' wing segment consisting of three linked nucleosides; and d) wherein the gap segment is positioned between the 5' wing segment and the 3' wing segment, and wherein each nucleoside of each wing segment comprises a cEt sugar.

[0438] Certain embodiments provide the use of any compound as described herein in the manufacture of a medicament for use in any of the therapeutic methods described herein. For example, certain embodiments provide the use of a compound as described herein in the manufacture of a medicament for treating, ameliorating, or preventing type 1 myotonic dystrophy. Certain embodiments provide the use of a compound as described herein in the manufacture of a medicament for inhibiting expression of DMPK and treating, preventing, delaying or ameliorating a DMPK related disease and or a symptom thereof. Certain embodiments provide the use of a compound as described herein in the manufacture of a medicament for reducing DMPK expression in an animal. Certain embodiments provide the use of a compound as described herein in the manufacture of a medicament for preferentially reducing CUGexp DMPK, reducing myotonia, or reducing spliceopathy in an animal. Certain embodiments provide the use of a compound as described herein in the manufacture of a medicament for treating an animal with type 1 myotonic dystrophy. Certain embodiments provide the use of a compound as described herein in the manufacture of a medicament for treating, preventing, delaying, or ameliorating symptoms and outcomes associated with development of DM1 including muscle stiffness, myotonia, disabling distal weakness, weakness in face and jaw muscles, difficulty in swallowing, drooping of the eyelids (ptosis), weakness of neck muscles, weakness in arm and leg muscles, persistent muscle pain, hypersomnia, muscle wasting, dysphagia, respiratory insufficiency, irregular heartbeat, heart muscle damage, apathy, insulin resistance, and cataracts. Certain embodiments provide the use of a compound as described herein in the manufacture of a medicament for counteracting RNA dominance by directing the cleavage of pathogenic transcripts.

[0439] Certain embodiments provide a kit for treating, preventing, or ameliorating type 1 myotonic dystrophy as described herein wherein the kit comprises: a) a compound as described herein; and optionally b) an additional agent or therapy as described herein. The kit can further include instructions or a label for using the kit to treat, prevent, or ameliorate type 1 myotonic dystrophy.

[0440] Certain embodiments provide any compound or composition as described herein, for use in any of the therapeutic methods described herein. For example, certain embodiments provide a compound or composition as described herein for inhibiting expression of DMPK and treating, preventing, delaying or ameliorating a DMPK related disease and or a symptom thereof. Certain embodiments provide a compound or composition as described herein for use in reducing DMPK expression in an animal. Certain embodiments provide a compound or composition as described herein for use in preferentially reducing CUGexp DMPK, reducing myotonia, or reducing spliceopathy in an animal. Certain embodiments provide a compound or composition as described herein for use in treating an animal with type 1 myotonic dystrophy. Certain embodiments provide a compound or composition as described herein for use in treating, preventing, delaying, or ameliorating symptoms and outcomes associated with development of DM1 including muscle stiffness, myotonia, disabling distal weakness, weakness in face and jaw muscles, difficulty in swallowing, drooping of the eyelids (ptosis), weakness of neck muscles, weakness in arm and leg muscles, persistent muscle pain, hypersomnia, muscle wasting, dysphagia, respiratory insufficiency, irregular heartbeat, heart muscle damage, apathy, insulin resistance, and cataracts. Certain embodiments provide a compound or composition as described herein for use in counteracting RNA dominance by directing the cleavage of pathogenic transcripts. Certain embodiments provide compounds comprising a modified oligonucleotide consisting of 12 to 30 linked nucleosides having a nucleobase sequence comprising at least 12 contiguous nucleobases of any of the nucleobase sequences of SEQ ID NOs: 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, or 33-874.

[0441] Other compounds which can be used in the methods described herein are also provided.

[0442] For example, certain embodiments provide compounds comprising a modified oligonucleotide consisting of 10 to 80, 12 to 50, 12 to 30, 15 to 30, 18 to 24, 19 to 22, or 20 linked nucleosides having a nucleobase sequence comprising at least 8, at least 9, at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, or at least 19, contiguous nucleobases of any of the nucleobase sequences of SEQ ID NOs: 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, or 33-874.

[0443] Certain embodiments provide compounds comprising a modified oligonucleotide consisting of 10 to 80, 12 to 50, 12 to 30, 15 to 30, 18 to 24, 19 to 22, or 20, linked nucleosides having a nucleobase sequence comprising at least 8, at least 9, at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, contiguous nucleobases of any of the nucleobase sequences of SEQ ID NOs: 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, or 33-874.

[0444] Certain embodiments provide compounds comprising a modified oligonucleotide consisting of 10 to 80, 12 to 50, 12 to 30, 15 to 30, or 15 to 17, linked nucleosides having a nucleobase sequence comprising a portion of at least 8, at least 9, at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, or at least 19, or more, contiguous nucleobases complementary to an equal length portion of nucleobases 1343-1368, 1317-1366, 2748-2791, 2155-2208, 2748-2791, 730-748, 528-547, 531-567, 636-697, 1311-1331, 1314-1339, 1446-1475, 1635-1670, 1610-1638, 1457-1486, 2773-1788, 931-948, 934-949, 937-952, 942-957, 937-957, 943-958, 937-953, 1346-1363, 1346-1361, 1347-1363, 2162-2179, 2492-2508, 2696-2717, or 2683-2703 of SEQ ID NO: 1.

[0445] Certain embodiments provide compounds comprising a modified oligonucleotide consisting of 10 to 80, 12 to 50, 12 to 30, 15 to 30, 18 to 24, 19 to 22, or 20, linked nucleosides having a nucleobase sequence comprising a portion of at least 8, at least 9, at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, or at least 19, or more, contiguous nucleobases complementary to an equal length portion of nucleobases 10195-10294, 13553-13572, 13748-13767, 13455-13475, 13628-13657, 13735-13760, 13746-13905, 13836-13851, 13553-13568, 13563-13578, 13624-13639, 13686-13701, 13760-13775, 13763-13779, 13765-13780, 2580-2595, 6446-6461, 11099-11115, 11082-11099, 1974-1993, 4435-4456, 6035-6052, 6360-6385, 6445-6468, 6807-6824, 6789-6806, or 6596-6615 of SEQ ID NO: 2.

[0446] In certain embodiments, the modified oligonucleotide is a single-stranded oligonucleotide.

[0447] In certain embodiments, the nucleobase sequence of the modified oligonucleotide is at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, or 100%, complementary to any of SEQ ID NOs: 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, or 33-874.

[0448] In certain embodiments, at least one internucleoside linkage is a modified internucleoside linkage.

[0449] In certain embodiments, each internucleoside linkage is a phosphorothioate internucleoside linkage.

[0450] In certain embodiments, at least one nucleoside comprises a modified sugar.

[0451] In certain embodiments, at least one modified sugar is a bicyclic sugar.

[0452] In certain embodiments, at least one modified sugar is a cEt.

[0453] In certain embodiments, at least one modified sugar comprises a 2'-O-methoxyethyl.

[0454] In certain embodiments, at least one nucleoside comprises a modified nucleobase.

[0455] In certain embodiments, the modified nucleobase is a 5-methylcytosine. In certain embodiments, each cytosine residue comprises a 5-methylcytosine.

[0456] In certain embodiments, the modified oligonucleotide consists of 16 linked nucleosides.

[0457] In certain embodiments, the modified oligonucleotide consists of 17 linked nucleosides.

[0458] In certain embodiments, the modified oligonucleotide consists of 20 linked nucleosides.

Antisense Compounds

[0459] Oligomeric compounds include, but are not limited to, oligonucleotides, oligonucleosides, oligonucleotide analogs, oligonucleotide mimetics, antisense compounds, antisense oligonucleotides, and siRNAs. An oligomeric compound can be "antisense" to a target nucleic acid, meaning that is capable of undergoing hybridization to a target nucleic acid through hydrogen bonding.

[0460] In certain embodiments, an antisense compound has a nucleobase sequence that, when written in the 5' to 3' direction, comprises the reverse complement of the target segment of a target nucleic acid to which it is targeted. In certain such embodiments, an antisense oligonucleotide has a nucleobase sequence that, when written in the 5' to 3' direction, comprises the reverse complement of the target segment of a target nucleic acid to which it is targeted.

[0461] In certain embodiments, an antisense compound targeted to DMPK as described herein is 10 to 30 nucleotides in length. In other words, the antisense compounds are in some embodiments from 10 to 30 linked nucleobases. In other embodiments, the antisense compound comprises a modified oligonucleotide consisting of 8 to 80, 10 to 80, 12 to 30, 12 to 50, 15 to 30, 15 to 18, 15 to 17, 16 to 16, 18 to 24, 19 to 22, or 20 linked nucleobases. In certain such embodiments, the antisense compound comprises a modified oligonucleotide consisting of 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, or 80 linked nucleobases in length, or a range defined by any two of the above values. In certain embodiments, antisense compounds of any of these lengths contain at least 8, at least 9, at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, or at least 19, contiguous nucleobases of the nucleobase sequence of any of the exemplary antisense compounds described herein (e.g., at least 8 contiguous nucleobases of a nucleobase sequence recited in any one of SEQ ID NOs: 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, or 33-874.

[0462] In certain embodiments, the antisense compound comprises a shortened or truncated modified oligonucleotide. The shortened or truncated modified oligonucleotide can have a single nucleoside deleted from the 5' end (5' truncation), or alternatively from the 3' end (3' truncation). A shortened or truncated oligonucleotide can have two nucleosides deleted from the 5' end, or alternatively can have two subunits deleted from the 3' end. Alternatively, the deleted nucleosides can be dispersed throughout the modified oligonucleotide, for example, in an antisense compound having one nucleoside deleted from the 5' end and one nucleoside deleted from the 3' end.

[0463] When a single additional nucleoside is present in a lengthened oligonucleotide, the additional nucleoside can be located at the 5' or 3' end of the oligonucleotide. When two or more additional nucleosides are present, the added nucleosides can be adjacent to each other, for example, in an oligonucleotide having two nucleosides added to the 5' end (5' addition), or alternatively to the 3' end (3' addition), of the oligonucleotide. Alternatively, the added nucleoside can be dispersed throughout the antisense compound, for example, in an oligonucleotide having one nucleoside added to the 5' end and one subunit added to the 3' end.

[0464] It is possible to increase or decrease the length of an antisense compound, such as an antisense oligonucleotide, and/or introduce mismatch bases without eliminating activity. For example, in Woolf et al. (Proc. Natl. Acad. Sci. USA 89:7305-7309, 1992), a series of antisense oligonucleotides 13-25 nucleobases in length were tested for their ability to induce cleavage of a target RNA in an oocyte injection model. Antisense oligonucleotides 25 nucleobases in length with 8 or 11 mismatch bases near the ends of the antisense oligonucleotides were able to direct specific cleavage of the target mRNA, albeit to a lesser extent than the antisense oligonucleotides that contained no mismatches. Similarly, target specific cleavage was achieved using 13 nucleobase antisense oligonucleotides, including those with 1 or 3 mismatches.

[0465] Gautschi et al (J. Natl. Cancer Inst. 93:463-471, March 2001) demonstrated the ability of an oligonucleotide having 100% complementarity to the bcl-2 mRNA and having 3 mismatches to the bcl-xL mRNA to reduce the expression of both bcl-2 and bcl-xL in vitro and in vivo. Furthermore, this oligonucleotide demonstrated potent anti-tumor activity in vivo.

[0466] Maher and Dolnick (Nuc. Acid. Res. 16:3341-3358, 1988) tested a series of tandem 14 nucleobase antisense oligonucleotides, and a 28 and 42 nucleobase antisense oligonucleotides comprised of the sequence of two or three of the tandem antisense oligonucleotides, respectively, for their ability to arrest translation of human DHFR in a rabbit reticulocyte assay. Each of the three 14 nucleobase antisense oligonucleotides alone was able to inhibit translation, albeit at a more modest level than the 28 or 42 nucleobase antisense oligonucleotides.

Target Nucleic Acids, Target Regions and Nucleotide Sequences

[0467] Nucleotide sequences that encode DMPK include, without limitation, the following sequences as set forth in GenBank Accession No. NM_001081560.1 (incorporated herein as SEQ ID NO: 1), GenBank Accession No. NT_011109.15 truncated from nucleotides 18540696 to Ser. No. 18/555,106 (incorporated herein as SEQ ID NO: 2), GenBank Accession No. NT_039413.7 truncated from nucleotides 16666001 to Ser. No. 16/681,000 (incorporated herein as SEQ ID NO: 3), GenBank Accession No. NM_032418.1 (incorporated herein as SEQ ID NO: 4), GenBank Accession No. AI007148.1 (incorporated herein as SEQ ID NO: 5), GenBank Accession No. AI304033.1 (incorporated herein as SEQ ID NO: 6), GenBank Accession No. BC024150.1 (incorporated herein as SEQ ID NO: 7), GenBank Accession No. BC056615.1 (incorporated herein as SEQ ID NO: 8), GenBank Accession No. BC075715.1 (incorporated herein as SEQ ID NO: 9), GenBank Accession No. BU519245.1 (incorporated herein as SEQ ID NO: 10), GenBank Accession No. CB247909.1 (incorporated herein as SEQ ID NO: 11), GenBank Accession No. CX208906.1 (incorporated herein as SEQ ID NO: 12), GenBank Accession No. CX732022.1 (incorporated herein as SEQ ID NO: 13), GenBank Accession No. S60315.1 (incorporated herein as SEQ ID NO: 14), GenBank Accession No. S60316.1 (incorporated herein as SEQ ID NO: 15), GenBank Accession No. NM_001081562.1 (incorporated herein as SEQ ID NO: 16), and GenBank Accession No. NM_001100.3 (incorporated herein as SEQ ID NO: 17). It is understood that the sequence set forth in each SEQ ID NO in the Examples contained herein is independent of any modification to a sugar moiety, an internucleoside linkage, or a nucleobase. As such, antisense compounds defined by a SEQ ID NO can comprise, independently, one or more modifications to a sugar moiety, an internucleoside linkage, or a nucleobase. Antisense compounds described by Isis Number (Isis No) indicate a combination of nucleobase sequence and motif.

[0468] In certain embodiments, a target region is a structurally defined region of the target nucleic acid. For example, a target region can encompass a 3' UTR, a 5' UTR, an exon, an intron, an exon/intron junction, a coding region, a translation initiation region, translation termination region, or other defined nucleic acid region. The structurally defined regions for DMPK can be obtained by accession number from sequence databases such as NCBI and such information is incorporated herein by reference. In certain embodiments, a target region can encompass the sequence from a 5' target site of one target segment within the target region to a 3' target site of another target segment within the target region.

[0469] Targeting includes determination of at least one target segment to which an antisense compound hybridizes, such that a desired effect occurs. In certain embodiments, the desired effect is a reduction in mRNA target nucleic acid levels. In certain embodiments, the desired effect is reduction of levels of protein encoded by the target nucleic acid or a phenotypic change associated with the target nucleic acid.

[0470] A target region can contain one or more target segments. Multiple target segments within a target region can be overlapping. Alternatively, they can be non-overlapping. In certain embodiments, target segments within a target region are separated by no more than about 300 nucleotides. In certain embodiments, target segments within a target region are separated by a number of nucleotides that is, is about, is no more than, is no more than about, 250, 200, 150, 100, 90, 80, 70, 60, 50, 40, 30, 20, or 10 nucleotides on the target nucleic acid, or is a range defined by any two of the preceding values. In certain embodiments, target segments within a target region are separated by no more than, or no more than about, 5 nucleotides on the target nucleic acid. In certain embodiments, target segments are contiguous. Contemplated are target regions defined by a range having a starting nucleic acid that is any of the 5' target sites or 3' target sites listed herein.

[0471] Suitable target segments can be found within a 5' UTR, a coding region, a 3' UTR, an intron, an exon, or an exon/intron junction. Target segments containing a start codon or a stop codon are also suitable target segments. A suitable target segment can specifically exclude a certain structurally defined region such as the start codon or stop codon.

[0472] The determination of suitable target segments can include a comparison of the sequence of a target nucleic acid to other sequences throughout the genome. For example, the BLAST algorithm can be used to identify regions of similarity amongst different nucleic acids. This comparison can prevent the selection of antisense compound sequences that can hybridize in a non-specific manner to sequences other than a selected target nucleic acid (i.e., non-target or off-target sequences).

[0473] There can be variation in activity (e.g., as defined by percent reduction of target nucleic acid levels) of the antisense compounds within an active target region. In certain embodiments, reductions in DMPK mRNA levels are indicative of inhibition of DMPK protein expression. Reductions in levels of a DMPK protein are also indicative of inhibition of target mRNA expression. Further, phenotypic changes, such as a reducing myotonia or reducing spliceopathy, can be indicative of inhibition of DMPK mRNA and/or protein expression.

Hybridization

[0474] In some embodiments, hybridization occurs between an antisense compound disclosed herein and a DMPK nucleic acid. The most common mechanism of hybridization involves hydrogen bonding (e.g., Watson-Crick, Hoogsteen or reversed Hoogsteen hydrogen bonding) between complementary nucleobases of the nucleic acid molecules.

[0475] Hybridization can occur under varying conditions. Stringent conditions are sequence-dependent and are determined by the nature and composition of the nucleic acid molecules to be hybridized.

[0476] Methods of determining whether a sequence is specifically hybridizable to a target nucleic acid are well known in the art (Sambrooke and Russell, Molecular Cloning: A Laboratory Manual, 3.sup.rd Ed., 2001). In certain embodiments, the antisense compounds provided herein are specifically hybridizable with a DMPK nucleic acid.

Complementarity

[0477] An antisense compound and a target nucleic acid are complementary to each other when a sufficient number of nucleobases of the antisense compound can hydrogen bond with the corresponding nucleobases of the target nucleic acid, such that a desired effect will occur (e.g., antisense inhibition of a target nucleic acid, such as a DMPK nucleic acid).

[0478] An antisense compound can hybridize over one or more segments of a DMPK nucleic acid such that intervening or adjacent segments are not involved in the hybridization event (e.g., a loop structure, mismatch or hairpin structure).

[0479] In certain embodiments, the antisense compounds provided herein, or a specified portion thereof, are, or are at least, 70%, 80%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100% complementary to a DMPK nucleic acid, a target region, target segment, or specified portion thereof. In certain embodiments, the antisense compounds are at least 70%, at least 80%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% complementary to a DMPK nucleic acid, a target region, target segment, or specified portion thereof, and contain at least 8, at least 9, at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, or at least 19, contiguous nucleobases of the nucleobase sequence of any of the exemplary antisense compounds described herein (e.g., at least 8 contiguous nucleobases of a nucleobase sequence recited in any one of SEQ ID NOs: 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, or 33-874). Percent complementarity of an antisense compound with a target nucleic acid can be determined using routine methods, and is measured over the entirety of the antisense compound.

[0480] For example, an antisense compound in which 18 of 20 nucleobases of the antisense compound are complementary to a target region, and would therefore specifically hybridize, would represent 90 percent complementarity. In this example, the remaining noncomplementary nucleobases can be clustered or interspersed with complementary nucleobases and need not be contiguous to each other or to complementary nucleobases. As such, an antisense compound which is 18 nucleobases in length having 4 (four) noncomplementary nucleobases which are flanked by two regions of complete complementarity with the target nucleic acid would have 77.8% overall complementarity with the target nucleic acid and would thus fall within the scope of the present invention. Percent complementarity of an antisense compound with a region of a target nucleic acid can be determined routinely using BLAST programs (basic local alignment search tools) and PowerBLAST programs known in the art (Altschul et al., J. Mol. Biol., 1990, 215, 403 410; Zhang and Madden, Genome Res., 1997, 7, 649 656). Percent homology, sequence identity or complementarity, can be determined by, for example, the Gap program (Wisconsin Sequence Analysis Package, Version 8 for Unix, Genetics Computer Group, University Research Park, Madison Wis.), using default settings, which uses the algorithm of Smith and Waterman (Adv. Appl. Math., 1981, 2, 482 489).

[0481] In certain embodiments, the antisense compounds provided herein, or specified portions thereof, are fully complementary (i.e. 100% complementary) to a target nucleic acid, or specified portion thereof. For example, antisense compound can be fully complementary to a DMPK nucleic acid, or a target region, or a target segment or target sequence thereof. As used herein, "fully complementary" means each nucleobase of an antisense compound is capable of precise base pairing with the corresponding nucleobases of a target nucleic acid. For example, a 20 nucleobase antisense compound is fully complementary to a target sequence that is 400 nucleobases long, so long as there is a corresponding 20 nucleobase portion of the target nucleic acid that is fully complementary to the antisense compound. Fully complementary can also be used in reference to a specified portion of the first and/or the second nucleic acid. For example, a 20 nucleobase portion of a 30 nucleobase antisense compound can be "fully complementary" to a target sequence that is 400 nucleobases long. The 20 nucleobase portion of the 30 nucleobase oligonucleotide is fully complementary to the target sequence if the target sequence has a corresponding 20 nucleobase portion wherein each nucleobase is complementary to the 20 nucleobase portion of the antisense compound. At the same time, the entire 30 nucleobase antisense compound can be fully complementary to the target sequence, depending on whether the remaining 10 nucleobases of the antisense compound are also complementary to the target sequence.

[0482] The location of a non-complementary nucleobase can be at the 5' end or 3' end of the antisense compound. Alternatively, the non-complementary nucleobase or nucleobases can be at an internal position of the antisense compound. When two or more non-complementary nucleobases are present, they can be either contiguous (i.e. linked) or non-contiguous. In one embodiment, a non-complementary nucleobase is located in the wing segment of a gapmer antisense oligonucleotide.

[0483] In certain embodiments, antisense compounds that are, or are up to 10, 12, 13, 14, 15, 16, 17, 18, 19, or 20 nucleobases in length comprise no more than 4, no more than 3, no more than 2, or no more than 1 non-complementary nucleobase(s) relative to a target nucleic acid, such as a DMPK nucleic acid, or specified portion thereof.

[0484] In certain embodiments, antisense compounds that are, or are up to 10, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, or 30 nucleobases in length comprise no more than 6, no more than 5, no more than 4, no more than 3, no more than 2, or no more than 1 non-complementary nucleobase(s) relative to a target nucleic acid, such as a DMPK nucleic acid, or specified portion thereof.

[0485] The antisense compounds provided herein also include those which are complementary to a portion of a target nucleic acid. As used herein, "portion" refers to a defined number of contiguous (i.e. linked) nucleobases within a region or segment of a target nucleic acid. A "portion" can also refer to a defined number of contiguous nucleobases of an antisense compound. In certain embodiments, the antisense compounds, are complementary to at least an 8 nucleobase portion of a target segment. In certain embodiments, the antisense compounds are complementary to at least a 10 nucleobase portion of a target segment. In certain embodiments, the antisense compounds are complementary to at least a 15 nucleobase portion of a target segment. Also contemplated are antisense compounds that are complementary to at least an 8, at least a 9, at least a 10, at least an 11, at least a 12, at least a 13, at least a 14, at least a 15, at least a 16, at least a 17, at least an 18, at least a 19, at least a 20, or more nucleobase portion of a target segment, or a range defined by any two of these values.

Identity

[0486] The antisense compounds provided herein can also have a defined percent identity to a particular nucleotide sequence, SEQ ID NO, or compound represented by a specific Isis number, or portion thereof. As used herein, an antisense compound is identical to the sequence disclosed herein if it has the same nucleobase pairing ability. For example, a RNA which contains uracil in place of thymidine in a disclosed DNA sequence would be considered identical to the DNA sequence since both uracil and thymidine pair with adenine. Shortened and lengthened versions of the antisense compounds described herein as well as compounds having non-identical bases relative to the antisense compounds provided herein also are contemplated. The non-identical bases can be adjacent to each other or dispersed throughout the antisense compound. Percent identity of an antisense compound is calculated according to the number of bases that have identical base pairing relative to the sequence to which it is being compared.

[0487] In certain embodiments, the antisense compounds, or portions thereof, are at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% or 100% identical to one or more of the exemplary antisense compounds or SEQ ID NOs, or a portion thereof, disclosed herein.

Modifications

[0488] A nucleoside is a base-sugar combination. The nucleobase (also known as base) portion of the nucleoside is normally a heterocyclic base moiety. Nucleotides are nucleosides that further include a phosphate group covalently linked to the sugar portion of the nucleoside. For those nucleosides that include a pentofuranosyl sugar, the phosphate group can be linked to the 2', 3' or 5' hydroxyl moiety of the sugar. Oligonucleotides are formed through the covalent linkage of adjacent nucleosides to one another, to form a linear polymeric oligonucleotide. Within the oligonucleotide structure, the phosphate groups are commonly referred to as forming the internucleoside linkages of the oligonucleotide.

[0489] Modifications to antisense compounds encompass substitutions or changes to internucleoside linkages, sugar moieties, or nucleobases. Modified antisense compounds are often preferred over native forms because of desirable properties such as, for example, enhanced cellular uptake, enhanced affinity for nucleic acid target, increased stability in the presence of nucleases, or increased inhibitory activity.

[0490] Chemically modified nucleosides can also be employed to increase the binding affinity of a shortened or truncated antisense oligonucleotide for its target nucleic acid. Consequently, comparable results can often be obtained with shorter antisense compounds that have such chemically modified nucleosides.

Modified Internucleoside Linkages

[0491] The naturally occurring internucleoside linkage of RNA and DNA is a 3' to 5' phosphodiester linkage. Antisense compounds having one or more modified, i.e. non-naturally occurring, internucleoside linkages are often selected over antisense compounds having naturally occurring internucleoside linkages because of desirable properties such as, for example, enhanced cellular uptake, enhanced affinity for target nucleic acids, and increased stability in the presence of nucleases.

[0492] Oligonucleotides having modified internucleoside linkages include internucleoside linkages that retain a phosphorus atom as well as internucleoside linkages that do not have a phosphorus atom. Representative phosphorus containing internucleoside linkages include, but are not limited to, phosphodiesters, phosphotriesters, methylphosphonates, phosphoramidate, and phosphorothioates. Methods of preparation of phosphorous-containing and non-phosphorous-containing linkages are well known.

[0493] In certain embodiments, antisense compounds targeted to a DMPK nucleic acid comprise one or more modified internucleoside linkages. In certain embodiments, the modified internucleoside linkages are phosphorothioate linkages. In certain embodiments, each internucleoside linkage of an antisense compound is a phosphorothioate internucleoside linkage.

Modified Sugar Moieties

[0494] Antisense compounds of the invention can optionally contain one or more nucleosides wherein the sugar group has been modified. Such sugar modified nucleosides may impart enhanced nuclease stability, increased binding affinity, or some other beneficial biological property to the antisense compounds. In certain embodiments, nucleosides comprise chemically modified ribofuranose ring moieties. Examples of chemically modified ribofuranose rings include without limitation, addition of substitutent groups (including 5' and 2' substituent groups, bridging of non-geminal ring atoms to form bicyclic nucleic acids (BNA), replacement of the ribosyl ring oxygen atom with S, N(R), or C(R.sub.1)(R.sub.2) (R, R.sub.1 and R.sub.2 are each independently H, C.sub.1-C.sub.12 alkyl or a protecting group) and combinations thereof. Examples of chemically modified sugars include 2'-F-5'-methyl substituted nucleoside (see PCT International Application WO 2008/101157 Published on Aug. 21, 2008 for other disclosed 5',2'-bis substituted nucleosides) or replacement of the ribosyl ring oxygen atom with S with further substitution at the 2'-position (see published U.S. Patent Application US2005-0130923, published on Jun. 16, 2005) or alternatively 5'-substitution of a BNA (see PCT International Application WO 2007/134181 Published on Nov. 22, 2007 wherein LNA is substituted with for example a 5'-methyl or a 5'-vinyl group).

[0495] Examples of nucleosides having modified sugar moieties include without limitation nucleosides comprising 5'-vinyl, 5'-methyl (R or S), 4'-S, 2'-F, 2'-OCH.sub.3, 2'-OCH.sub.2CH.sub.3, 2'-OCH.sub.2CH.sub.2F and 2'-O(CH.sub.2).sub.2OCH.sub.3 substituent groups. The substituent at the 2' position can also be selected from allyl, amino, azido, thio, O-allyl, O--C.sub.1-C.sub.10 alkyl, OCF.sub.3, OCH.sub.2F, O(CH.sub.2).sub.2SCH.sub.3, O(CH.sub.2).sub.2--O--N(R.sub.m)(R.sub.n), O--CH.sub.2--C(.dbd.O)--N(R.sub.m)(R.sub.n), and O--CH.sub.2--C(.dbd.O)--N(R.sub.l)--(CH.sub.2).sub.2--N(R.sub.m)(R.sub.n)- , where each R.sub.l, R.sub.m and R.sub.n is, independently, H or substituted or unsubstituted C.sub.1-C.sub.10 alkyl.

[0496] Examples of bicyclic nucleic acids (BNAs) include without limitation nucleosides comprising a bridge between the 4' and the 2' ribosyl ring atoms. In certain embodiments, antisense compounds provided herein include one or more BNA nucleosides wherein the bridge comprises one of the formulas: 4'-(CH.sub.2)--O-2' (LNA); 4'-(CH.sub.2)--S-2; 4'-(CH.sub.2).sub.2--O-2' (ENA); 4'-CH(CH.sub.3)--O-2' and 4'-CH(CH.sub.2OCH.sub.3)--O-2' (and analogs thereof see U.S. Pat. No. 7,399,845, issued on Jul. 15, 2008); 4'-C(CH.sub.3)(CH.sub.3)--O-2' (and analogs thereof see PCT/US2008/068922 published as WO/2009/006478, published Jan. 8, 2009); 4'-CH.sub.2--N(OCH.sub.3)-2' (and analogs thereof see PCT/US2008/064591 published as WO/2008/150729, published Dec. 11, 2008); 4'-CH.sub.2--O--N(CH.sub.3)-2' (see published U.S. Patent Application US2004-0171570, published Sep. 2, 2004); 4'-CH.sub.2--N(R)--O-2', wherein R is H, C.sub.1-C.sub.12 alkyl, or a protecting group (see U.S. Pat. No. 7,427,672, issued on Sep. 23, 2008); 4'-CH.sub.2--C(H)(CH.sub.3)-2' (see Chattopadhyaya et al., J. Org. Chem., 2009, 74, 118-134); and 4'-CH.sub.2--C(.dbd.CH.sub.2)-2' (and analogs thereof see PCT/US2008/066154 published as WO 2008/154401, published on Dec. 8, 2008).

[0497] Further bicyclic nucleosides have been reported in published literature (see for example: Srivastava et al., J. Am. Chem. Soc., 2007, 129(26) 8362-8379; Frieden et al., Nucleic Acids Research, 2003, 21, 6365-6372; Elayadi et al., Curr. Opinion Invens. Drugs, 2001, 2, 558-561; Braasch et al., Chem. Biol., 2001, 8, 1-7; Orum et al., Curr. Opinion Mol. Ther., 2001, 3, 239-243; Wahlestedt et al., Proc. Natl. Acad. Sci. U.S.A, 2000, 97, 5633-5638; Singh et al., Chem. Commun., 1998, 4, 455-456; Koshkin et al., Tetrahedron, 1998, 54, 3607-3630; Kumar et al., Bioorg. Med. Chem. Lett., 1998, 8, 2219-2222; Singh et al., J. Org. Chem., 1998, 63, 10035-10039; U.S. Pat. Nos. 7,399,845; 7,053,207; 7,034,133; 6,794,499; 6,770,748; 6,670,461; 6,525,191; 6,268,490; U.S. Patent Publication Nos.: US2008-0039618; US2007-0287831; US2004-0171570; U.S. patent application Ser. No. 12/129,154; 61/099,844; 61/097,787; 61/086,231; 61/056,564; 61/026,998; 61/026,995; 60/989,574; International applications WO 2007/134181; WO 2005/021570; WO 2004/106356; WO 94/14226; and PCT International Applications Nos.: PCT/US2008/068922; PCT/US2008/066154; and PCT/US2008/064591). Each of the foregoing bicyclic nucleosides can be prepared having one or more stereochemical sugar configurations including for example .alpha.-L-ribofuranose and .beta.-D-ribofuranose (see PCT international application PCT/DK98/00393, published on Mar. 25, 1999 as WO 99/14226).

[0498] In certain embodiments, bicyclic nucleosides comprise a bridge between the 4' and the 2' carbon atoms of the pentofuranosyl sugar moiety including without limitation, bridges comprising 1 or from 1 to 4 linked groups independently selected from --[C(R.sub.a)(R.sub.b)].sub.n--, --C(R.sub.a).dbd.C(R.sub.b)--, --C(R.sub.a).dbd.N--, --C(.dbd.NR.sub.a)--, --C(.dbd.O)--, --C(.dbd.S)--, --O--, --Si(R.sub.a).sub.2--, --S(.dbd.O).sub.x--, and --N(R.sub.a)--; wherein: x is 0, 1, or 2; n is 1, 2, 3, or 4; each R.sub.a and R.sub.b is, independently, H, a protecting group, hydroxyl, C.sub.1-C.sub.12 alkyl, substituted C.sub.1-C.sub.12 alkyl, C.sub.2-C.sub.12 alkenyl, substituted C.sub.2-C.sub.12 alkenyl, C.sub.2-C.sub.12 alkynyl, substituted C.sub.2-C.sub.12 alkynyl, C.sub.5-C.sub.20 aryl, substituted C.sub.5-C.sub.20 aryl, heterocycle radical, substituted heterocycle radical, heteroaryl, substituted heteroaryl, C.sub.5-C.sub.7 alicyclic radical, substituted C.sub.5-C.sub.7 alicyclic radical, halogen, OJ.sub.1, NJ.sub.1J.sub.2, SJ.sub.1, N.sub.3, COOJ.sub.1, acyl (C(.dbd.O)--H), substituted acyl, CN, sulfonyl (S(.dbd.O).sub.2-J.sub.1), or sulfoxyl (S(.dbd.O)-J.sub.1); and

[0499] each J.sub.1 is, independently, H, C.sub.1-C.sub.12 alkyl, substituted C.sub.1-C.sub.12 alkyl, C.sub.2-C.sub.12 alkenyl, substituted C.sub.2-C.sub.12 alkenyl, C.sub.2-C.sub.12 alkynyl, substituted C.sub.2-C.sub.12 alkynyl, C.sub.5-C.sub.20 aryl, substituted C.sub.5-C.sub.20 aryl, acyl (C(.dbd.O)--H), substituted acyl, a heterocycle radical, a substituted heterocycle radical, C.sub.1-C.sub.12 aminoalkyl, substituted C.sub.1-C.sub.12 aminoalkyl or a protecting group.

[0500] In certain embodiments, the bridge of a bicyclic sugar moiety is, --[C(R.sub.a)(R.sub.b)].sub.n--, --[C(R.sub.a)(R.sub.b)].sub.n--O--, --C(R.sub.aR.sub.b)--N(R)--O-- or --C(R.sub.aR.sub.b)--O--N(R)--. In certain embodiments, the bridge is 4'-CH.sub.2-2', 4'-(CH.sub.2).sub.2-2', 4'-(CH.sub.2).sub.3-2', 4'-CH.sub.2--O-2', 4'-(CH.sub.2).sub.2--O-2', 4'-CH.sub.2--O--N(R)-2' and 4'-CH.sub.2--N(R)--O-2'- wherein each R is, independently, H, a protecting group or C.sub.1-C.sub.12 alkyl.

[0501] In certain embodiments, bicyclic nucleosides are further defined by isomeric configuration. For example, a nucleoside comprising a 4'-(CH.sub.2)--O-2' bridge, may be in the .alpha.-L configuration or in the .beta.-D configuration. Previously, .alpha.-L-methyleneoxy (4'-CH.sub.2--O-2) BNA's have been incorporated into antisense oligonucleotides that showed antisense activity (Frieden et al., Nucleic Acids Research, 2003, 21, 6365-6372).

[0502] In certain embodiments, bicyclic nucleosides include those having a 4' to 2' bridge wherein such bridges include without limitation, .alpha.-L-4'-(CH.sub.2)--O-2', .beta.-D-4'-CH.sub.2--O-2', 4'-(CH.sub.2).sub.2--O-2', 4'-CH.sub.2--O--N(R)-2', 4'-CH.sub.2--N(R)--O-2', 4'-CH(CH.sub.3)--O-2', 4'-CH.sub.2--S-2', 4'-CH.sub.2--N(R)-2', 4'-CH.sub.2--CH(CH.sub.3)-2', and 4'-(CH.sub.2).sub.3-2', wherein R is H, a protecting group or C.sub.1-C.sub.12 alkyl.

[0503] In certain embodiments, bicyclic nucleosides have the formula:

##STR00001##

wherein:

[0504] Bx is a heterocyclic base moiety;

[0505] -Q.sub.a-Q.sub.b-Q.sub.c- is --CH.sub.2--N(R.sub.c)--CH.sub.2--, --C(.dbd.O)--N(R.sub.c)--CH.sub.2--, --CH.sub.2--O--N(R.sub.c)--, --CH.sub.2--N(R.sub.c)--O-- or --N(R.sub.c)--O--CH.sub.2;

[0506] R.sub.c is C.sub.1-C.sub.12 alkyl or an amino protecting group; and

[0507] T.sub.a and T.sub.b are each, independently H, a hydroxyl protecting group, a conjugate group, a reactive phosphorus group, a phosphorus moiety or a covalent attachment to a support medium.

[0508] In certain embodiments, bicyclic nucleosides have the formula:

##STR00002##

wherein:

[0509] Bx is a heterocyclic base moiety;

[0510] T.sub.a and T.sub.b are each, independently H, a hydroxyl protecting group, a conjugate group, a reactive phosphorus group, a phosphorus moiety or a covalent attachment to a support medium;

[0511] Z.sub.a is C.sub.1-C.sub.6 alkyl, C.sub.2-C.sub.6 alkenyl, C.sub.2-C.sub.6 alkynyl, substituted C.sub.1-C.sub.6 alkyl, substituted C.sub.2-C.sub.6 alkenyl, substituted C.sub.2-C.sub.6 alkynyl, acyl, substituted acyl, substituted amide, thiol or substituted thiol.

[0512] In one embodiment, each of the substituted groups, is, independently, mono or poly substituted with substituent groups independently selected from halogen, oxo, hydroxyl, OJ.sub.c, NJ.sub.cJ.sub.d, SJ.sub.c, N.sub.3, OC(.dbd.X)J.sub.c, and NJ.sub.eC(.dbd.X)NJ.sub.cJ.sub.d, wherein each J.sub.c, J.sub.d and J.sub.e is, independently, H, C.sub.1-C.sub.6 alkyl, or substituted C.sub.1-C.sub.6 alkyl and X is O or NJ.sub.c.

[0513] In certain embodiments, bicyclic nucleosides have the formula:

##STR00003##

wherein:

[0514] Bx is a heterocyclic base moiety;

[0515] T.sub.a and T.sub.b are each, independently H, a hydroxyl protecting group, a conjugate group, a reactive phosphorus group, a phosphorus moiety or a covalent attachment to a support medium;

[0516] Z.sub.b is C.sub.1-C.sub.6 alkyl, C.sub.2-C.sub.6 alkenyl, C.sub.2-C.sub.6 alkynyl, substituted C.sub.1-C.sub.6 alkyl, substituted C.sub.2-C.sub.6 alkenyl, substituted C.sub.2-C.sub.6 alkynyl or substituted acyl (C(.dbd.O)--).

[0517] In certain embodiments, bicyclic nucleosides have the formula:

##STR00004##

wherein:

[0518] Bx is a heterocyclic base moiety;

[0519] T.sub.a and T.sub.b are each, independently H, a hydroxyl protecting group, a conjugate group, a reactive phosphorus group, a phosphorus moiety or a covalent attachment to a support medium;

[0520] R.sub.d is C.sub.1-C.sub.6 alkyl, substituted C.sub.1-C.sub.6 alkyl, C.sub.2-C.sub.6 alkenyl, substituted C.sub.2-C.sub.6 alkenyl, C.sub.2-C.sub.6 alkynyl or substituted C.sub.2-C.sub.6 alkynyl; [0521] each q.sub.a, q.sub.b, q.sub.c and q.sub.d is, independently, H, halogen, C.sub.1-C.sub.6 alkyl, substituted C.sub.1-C.sub.6 alkyl, C.sub.2-C.sub.6 alkenyl, substituted C.sub.2-C.sub.6 alkenyl, C.sub.2-C.sub.6 alkynyl or substituted C.sub.2-C.sub.6 alkynyl, C.sub.1-C.sub.6 alkoxyl, substituted C.sub.1-C.sub.6 alkoxyl, acyl, substituted acyl, C.sub.1-C.sub.6 aminoalkyl or substituted C.sub.1-C.sub.6 aminoalkyl;

[0522] In certain embodiments, bicyclic nucleosides have the formula:

##STR00005##

wherein:

[0523] Bx is a heterocyclic base moiety;

[0524] T.sub.a and T.sub.b are each, independently H, a hydroxyl protecting group, a conjugate group, a reactive phosphorus group, a phosphorus moiety or a covalent attachment to a support medium;

[0525] q.sub.a, q.sub.b, q.sub.c and q.sub.f are each, independently, hydrogen, halogen, C.sub.1-C.sub.12 alkyl, substituted C.sub.1-C.sub.12 alkyl, C.sub.2-C.sub.12 alkenyl, substituted C.sub.2-C.sub.12 alkenyl, C.sub.2-C.sub.12 alkynyl, substituted C.sub.2-C.sub.12 alkynyl, C.sub.1-C.sub.12 alkoxy, substituted C.sub.1-C.sub.12 alkoxy, OJ.sub.j, SJ.sub.j, SOJ.sub.j, SO.sub.2J.sub.j, NJ.sub.jJ.sub.k, N.sub.3, CN, C(.dbd.O)OJ.sub.j, C(.dbd.O)NJ.sub.jJ.sub.k, C(.dbd.O)J.sub.j, O--C(.dbd.O)NJ.sub.jJ.sub.k, N(H)C(.dbd.NH)NJ.sub.jJ.sub.k, N(H)C(.dbd.O)NJ.sub.jJ.sub.k or N(H)C(.dbd.S)NJ.sub.jJ.sub.k;

[0526] or q.sub.e and q.sub.f together are .dbd.C(q.sub.g)(q.sub.h);

[0527] q.sub.g and q.sub.h are each, independently, H, halogen, C.sub.1-C.sub.12 alkyl or substituted C.sub.1-C.sub.12 alkyl.

[0528] The synthesis and preparation of adenine, cytosine, guanine, 5-methyl-cytosine, thymine and uracil bicyclic nucleosides having a 4'-CH.sub.2--O-2' bridge, along with their oligomerization, and nucleic acid recognition properties have been described (Koshkin et al., Tetrahedron, 1998, 54, 3607-3630). The synthesis of bicyclic nucleosides has also been described in WO 98/39352 and WO 99/14226.

[0529] Analogs of various bicyclic nucleosides that have 4' to 2' bridging groups such as 4'-CH.sub.2--O-2' and 4'-CH.sub.2--S-2', have also been prepared (Kumar et al., Bioorg. Med. Chem. Lett., 1998, 8, 2219-2222). Preparation of oligodeoxyribonucleotide duplexes comprising bicyclic nucleosides for use as substrates for nucleic acid polymerases has also been described (Wengel et al., WO 99/14226). Furthermore, synthesis of 2'-amino-BNA, a novel conformationally restricted high-affinity oligonucleotide analog has been described in the art (Singh et al., J. Org. Chem., 1998, 63, 10035-10039). In addition, 2'-amino- and 2'-methylamino-BNA's have been prepared and the thermal stability of their duplexes with complementary RNA and DNA strands has been previously reported.

[0530] In certain embodiments, bicyclic nucleosides have the formula:

##STR00006##

wherein:

[0531] Bx is a heterocyclic base moiety;

[0532] T.sub.a and T.sub.b are each, independently H, a hydroxyl protecting group, a conjugate group, a reactive phosphorus group, a phosphorus moiety or a covalent attachment to a support medium;

[0533] each q.sub.i, q.sub.j, q.sub.k and q.sub.l is, independently, H, halogen, C.sub.1-C.sub.12 alkyl, substituted C.sub.1-C.sub.12 alkyl, C.sub.2-C.sub.12 alkenyl, substituted C.sub.2-C.sub.12 alkenyl, C.sub.2-C.sub.12 alkynyl, substituted C.sub.2-C.sub.12 alkynyl, C.sub.1-C.sub.12 alkoxyl, substituted C.sub.1-C.sub.12 alkoxyl, OJ.sub.j, SJ.sub.j, SOJ.sub.j, SO.sub.2J.sub.j, NJ.sub.jJ.sub.k, N.sub.3, CN, C(.dbd.O)OJ.sub.j, C(.dbd.O)NJ.sub.jJ.sub.k, C(.dbd.O)J.sub.j, O--C(.dbd.O)NJ.sub.jJ.sub.k, N(H)C(.dbd.NH)NJ.sub.jJ.sub.k, N(H)C(.dbd.O)NJ.sub.jJ.sub.k or N(H)C(.dbd.S)NJ.sub.jJ.sub.k; and

[0534] q.sub.i and q.sub.j or q.sub.l and q.sub.k together are .dbd.C(q.sub.g)(q.sub.h), wherein q.sub.g and q.sub.h are each, independently, H, halogen, C.sub.1-C.sub.12 alkyl or substituted C.sub.1-C.sub.12 alkyl.

[0535] One carbocyclic bicyclic nucleoside having a 4'-(CH.sub.2).sub.3-2' bridge and the alkenyl analog bridge 4'-CH.dbd.CH--CH.sub.2-2' have been described (Frier et al., Nucleic Acids Research, 1997, 25(22), 4429-4443 and Albaek et al., J. Org. Chem., 2006, 71, 7731-7740). The synthesis and preparation of carbocyclic bicyclic nucleosides along with their oligomerization and biochemical studies have also been described (Srivastava et al., J. Am. Chem. Soc. 2007, 129(26), 8362-8379).

[0536] In certain embodiments, bicyclic nucleosides include, but are not limited to, (A) .alpha.-L-methyleneoxy (4'-CH.sub.2--O-2) BNA, (B) .beta.-D-methyleneoxy (4'-CH.sub.2--O-2) BNA, (C) ethyleneoxy (4'-(CH.sub.2).sub.2--O-2') BNA, (D) aminooxy (4'-CH.sub.2--O--N(R)-2') BNA, (E) oxyamino (4'-CH.sub.2--N(R)--O-2') BNA, (F) methyl(methyleneoxy) (4'-CH(CH.sub.3)--O-2) BNA (also referred to as constrained ethyl or cEt), (G) methylene-thio (4'-CH.sub.2--S-2) BNA, (H) methylene-amino (4'-CH.sub.2--N(R)-2) BNA, (I) methyl carbocyclic (4'-CH.sub.2--CH(CH.sub.3)-2') BNA, (J) propylene carbocyclic (4'-(CH.sub.2).sub.3-2') BNA, and (K) vinyl BNA as depicted below.

##STR00007## ##STR00008##

wherein Bx is the base moiety and R is, independently, H, a protecting group, C.sub.1-C.sub.6 alkyl or C.sub.1-C.sub.6 alkoxy.

[0537] In certain embodiments, nucleosides are modified by replacement of the ribosyl ring with a sugar surrogate. Such modification includes without limitation, replacement of the ribosyl ring with a surrogate ring system (sometimes referred to as DNA analogs) such as a morpholino ring, a cyclohexenyl ring, a cyclohexyl ring or a tetrahydropyranyl ring such as one having one of the formula:

##STR00009##

[0538] In certain embodiments, sugar surrogates are selected having the formula:

##STR00010##

wherein:

[0539] Bx is a heterocyclic base moiety;

[0540] T.sub.3 and T.sub.4 are each, independently, an internucleoside linking group linking the tetrahydropyran nucleoside analog to the oligomeric compound or one of T.sub.3 and T.sub.4 is an internucleoside linking group linking the tetrahydropyran nucleoside analog to an oligomeric compound or oligonucleotide and the other of T.sub.3 and T.sub.4 is H, a hydroxyl protecting group, a linked conjugate group or a 5' or 3'-terminal group;

q.sub.1, q.sub.2, q.sub.3, q.sub.4, q.sub.5, q.sub.6 and q.sub.7 are each independently, H, C.sub.1-C.sub.6 alkyl, substituted C.sub.1-C.sub.6 alkyl, C.sub.2-C.sub.6 alkenyl, substituted C.sub.2-C.sub.6 alkenyl, C.sub.2-C.sub.6 alkynyl or substituted C.sub.2-C.sub.6 alkynyl; and

[0541] one of R.sub.1 and R.sub.2 is hydrogen and the other is selected from halogen, substituted or unsubstituted alkoxy, NJ.sub.1J.sub.2, SJ.sub.1, N.sub.3, OC(.dbd.X)J.sub.1, OC(.dbd.X)NJ.sub.1J.sub.2, NJ.sub.3C(.dbd.X)NJ.sub.1J.sub.2 and CN, wherein X is O, S or NJ.sub.1 and each J.sub.1, J.sub.2 and J.sub.3 is, independently, H or C.sub.1-C.sub.6 alkyl.

[0542] In certain embodiments, q.sub.1, q.sub.2, q.sub.3, q.sub.4, q.sub.5, q.sub.6 and q.sub.7 are each H. In certain embodiments, at least one of q.sub.1, q.sub.2, q.sub.3, q.sub.4, q.sub.5, q.sub.6 and q.sub.7 is other than H. In certain embodiments, at least one of q.sub.1, q.sub.2, q.sub.3, q.sub.4, q.sub.5, q.sub.6 and q.sub.7 is methyl. In certain embodiments, THP nucleosides are provided wherein one of R.sub.1 and R.sub.2 is F. In certain embodiments, R.sub.1 is fluoro and R.sub.2 is H; R.sub.1 is methoxy and R.sub.2 is H, and R.sub.1 is methoxyethoxy and R.sub.2 is H.

[0543] Such sugar surrogates include, but are not limited to, what is referred to in the art as hexitol nucleic acid (HNA), altritol nucleic acid (ANA), and mannitol nucleic acid (MNA) (see Leumann, C. J., Bioorg. & Med. Chem., 2002, 10, 841-854).

[0544] In certain embodiments, sugar surrogates comprise rings having more than 5 atoms and more than one heteroatom. For example nucleosides comprising morpholino sugar moieties and their use in oligomeric compounds has been reported (see for example: Braasch et al., Biochemistry, 2002, 41, 4503-4510; and U.S. Pat. Nos. 5,698,685; 5,166,315; 5,185,444; and 5,034,506).

[0545] As used here, the term "morpholino" means a sugar surrogate having the following structure:

##STR00011##

[0546] In certain embodiments, morpholinos may be modified, for example by adding or altering various substituent groups from the above morpholino structure. Such sugar surrogates are referred to herein as "modified morpholinos."

[0547] In certain embodiments, antisense compounds comprise one or more modified cyclohexenyl nucleosides, which is a nucleoside having a six-membered cyclohexenyl in place of the pentofuranosyl residue in naturally occurring nucleosides. Modified cyclohexenyl nucleosides include, but are not limited to those described in the art (see for example commonly owned, published PCT Application WO 2010/036696, published on Apr. 10, 2010, Robeyns et al., J. Am. Chem. Soc., 2008, 130(6), 1979-1984; Horvath et al., Tetrahedron Letters, 2007, 48, 3621-3623; Nauwelaerts et al., J. Am. Chem. Soc., 2007, 129(30), 9340-9348; Gu et al. Nucleosides, Nucleotides & Nucleic Acids, 2005, 24(5-7), 993-998; Nauwelaerts et al., Nucleic Acids Research, 2005, 33(8), 2452-2463; Robeyns et al., Acta Crystallographica, Section F: Structural Biology and Crystallization Communications, 2005, F61(6), 585-586; Gu et al., Tetrahedron, 2004, 60(9), 2111-2123; Gu et al., Oligonucleotides, 2003, 13(6), 479-489; Wang et al., J. Org. Chem., 2003, 68, 4499-4505; Verbeure et al., Nucleic Acids Research, 2001, 29(24), 4941-4947; Wang et al., J. Org. Chem., 2001, 66, 8478-82; Wang et al., Nucleosides, Nucleotides & Nucleic Acids, 2001, 20(4-7), 785-788; Wang et al., J. Am. Chem., 2000, 122, 8595-8602; Published PCT application, WO 06/047842; and Published PCT Application WO 01/049687; the text of each is incorporated by reference herein, in their entirety). Certain modified cyclohexenyl nucleosides have the formula:

##STR00012##

wherein:

[0548] Bx is a heterocyclic base moiety;

[0549] T.sub.3 and T.sub.4 are each, independently, an internucleoside linking group linking the cyclohexenyl nucleoside analog to an antisense compound or one of T.sub.3 and T.sub.4 is an internucleoside linking group linking the tetrahydropyran nucleoside analog to an antisense compound and the other of T.sub.3 and T.sub.4 is H, a hydroxyl protecting group, a linked conjugate group, or a 5'- or 3'-terminal group; and q.sub.1, q.sub.2, q.sub.3, q.sub.4, q.sub.5, q.sub.6, q.sub.7, q.sub.8 and q.sub.9 are each, independently, H, C.sub.1-C.sub.6 alkyl, substituted C.sub.1-C.sub.6 alkyl, C.sub.2-C.sub.6 alkenyl, substituted C.sub.2-C.sub.6 alkenyl, C.sub.2-C.sub.6 alkynyl, substituted C.sub.2-C.sub.6 alkynyl or other sugar substituent group.

[0550] Many other bicyclic and tricyclic sugar surrogate ring systems are also known in the art that can be used to modify nucleosides for incorporation into antisense compounds (see for example review article: Leumann, Christian J., Bioorg. & Med. Chem., 2002, 10, 841-854). Such ring systems can undergo various additional substitutions to enhance activity.

[0551] Methods for the preparations of modified sugars are well known to those skilled in the art. Some representative U.S. patents that teach the preparation of such modified sugars include without limitation, U.S.: 4,981,957; 5,118,800; 5,319,080; 5,359,044; 5,393,878; 5,446,137; 5,466,786; 5,514,785; 5,519,134; 5,567,811; 5,576,427; 5,591,722; 5,597,909; 5,610,300; 5,627,053; 5,639,873; 5,646,265; 5,670,633; 5,700,920; 5,792,847 and 6,600,032 and International Application PCT/US2005/019219, filed Jun. 2, 2005 and published as WO 2005/121371 on Dec. 22, 2005, and each of which is herein incorporated by reference in its entirety.

[0552] In nucleotides having modified sugar moieties, the nucleobase moieties (natural, modified or a combination thereof) are maintained for hybridization with an appropriate nucleic acid target.

[0553] In certain embodiments, antisense compounds targeted to a DMPK nucleic acid comprise one or more nucleotides having modified sugar moieties. In certain embodiments, the modified sugar moiety is 2'-MOE. In certain embodiments, the 2'-MOE modified nucleotides are arranged in a gapmer motif.

Modified Nucleobases

[0554] Nucleobase (or base) modifications or substitutions are structurally distinguishable from, yet functionally interchangeable with, naturally occurring or synthetic unmodified nucleobases. Both natural and modified nucleobases are capable of participating in hydrogen bonding. Such nucleobase modifications can impart nuclease stability, binding affinity or some other beneficial biological property to antisense compounds. Modified nucleobases include synthetic and natural nucleobases such as, for example, 5-methylcytosine (5-me-C). Certain nucleobase substitutions, including 5-methylcytosine substitutions, are particularly useful for increasing the binding affinity of an antisense compound for a target nucleic acid. For example, 5-methylcytosine substitutions have been shown to increase nucleic acid duplex stability by 0.6-1.2.degree. C. (Sanghvi, Y. S., Crooke, S. T. and Lebleu, B., eds., Antisense Research and Applications, CRC Press, Boca Raton, 1993, pp. 276-278).

[0555] Additional unmodified nucleobases include 5-hydroxymethyl cytosine, xanthine, hypoxanthine, 2-aminoadenine, 6-methyl and other alkyl derivatives of adenine and guanine, 2-propyl and other alkyl derivatives of adenine and guanine, 2-thiouracil, 2-thiothymine and 2-thiocytosine, 5-halouracil and cytosine, 5-propynyl (--C.ident.C--CH.sub.3) uracil and cytosine and other alkynyl derivatives of pyrimidine bases, 6-azo uracil, cytosine and thymine, 5-uracil (pseudouracil), 4-thiouracil, 8-halo, 8-amino, 8-thiol, 8-thioalkyl, 8-hydroxyl and other 8-substituted adenines and guanines, 5-halo particularly 5-bromo, 5-trifluoromethyl and other 5-substituted uracils and cytosines, 7-methylguanine and 7-methyladenine, 2-F-adenine, 2-amino-adenine, 8-azaguanine and 8-azaadenine, 7-deazaguanine and 7-deazaadenine and 3-deazaguanine and 3-deazaadenine.

[0556] Heterocyclic base moieties can also include those in which the purine or pyrimidine base is replaced with other heterocycles, for example 7-deaza-adenine, 7-deazaguanosine, 2-aminopyridine and 2-pyridone. Nucleobases that are particularly useful for increasing the binding affinity of antisense compounds include 5-substituted pyrimidines, 6-azapyrimidines and N-2, N-6 and 0-6 substituted purines, including 2 aminopropyladenine, 5-propynyluracil and 5-propynylcytosine.

[0557] In certain embodiments, antisense compounds targeted to a DMPK nucleic acid comprise one or more modified nucleobases. In certain embodiments, gap-widened antisense oligonucleotides targeted to a DMPK nucleic acid comprise one or more modified nucleobases. In certain embodiments, the modified nucleobase is 5-methylcytosine. In certain embodiments, each cytosine is a 5-methylcytosine.

Certain Antisense Compound Motifs

[0558] In certain embodiments, antisense compounds targeted to a DMPK nucleic acid have chemically modified subunits arranged in patterns, or motifs, to confer to the antisense compounds properties such as enhanced the inhibitory activity, increased binding affinity for a target nucleic acid, or resistance to degradation by in vivo nucleases.

[0559] Chimeric antisense compounds typically contain at least one region modified so as to confer increased resistance to nuclease degradation, increased cellular uptake, increased binding affinity for the target nucleic acid, and/or increased inhibitory activity. A second region of a chimeric antisense compound can optionally serve as a substrate for the cellular endonuclease RNase H, which cleaves the RNA strand of an RNA:DNA duplex.

[0560] Antisense compounds having a gapmer motif are considered chimeric antisense compounds. In a gapmer an internal region having a plurality of nucleotides that supports RNaseH cleavage is positioned between external regions having a plurality of nucleotides that are chemically distinct from the nucleosides of the internal region. In the case of an antisense oligonucleotide having a gapmer motif, the gap segment generally serves as the substrate for endonuclease cleavage, while the wing segments comprise modified nucleosides. In certain embodiments, the regions of a gapmer are differentiated by the types of sugar moieties comprising each distinct region. The types of sugar moieties that are used to differentiate the regions of a gapmer can in some embodiments include .beta.-D-ribonucleosides, .beta.-D-deoxyribonucleosides, 2'-modified nucleosides (such 2'-modified nucleosides can include 2'-MOE, and 2'-O--CH.sub.3, among others), and bicyclic sugar modified nucleosides (such bicyclic sugar modified nucleosides can include those having a 4'-(CH.sub.2).sub.n--O-2' bridge, where n=1 or n=2). The wing-gap-wing motif is frequently described as "X--Y--Z", where "X" represents the length of the 5' wing region, "Y" represents the length of the gap region, and "Z" represents the length of the 3' wing region. As used herein, a gapmer described as "X--Y--Z" has a configuration such that the gap segment is positioned immediately adjacent each of the 5' wing segment and the 3' wing segment. Thus, no intervening nucleotides exist between the 5' wing segment and gap segment, or the gap segment and the 3' wing segment. Any of the antisense compounds described herein can have a gapmer motif. In some embodiments, X and Z are the same, in other embodiments they are different. In a preferred embodiment, Y is between 8 and 15 nucleotides. X, Y or Z can be any of 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 25, 30 or more nucleotides. Thus, gapmers include, but are not limited to, for example 5-10-5, 4-8-4, 4-12-3, 4-12-4, 3-14-3, 2-.beta.-5, 2-16-2, 1-18-1, 3-10-3, 2-10-2, 1-10-1, 2-8-2, 6-8-6, 5-8-5, 5-7-5, 1-8-1, or 2-6-2.

[0561] In certain embodiments, the antisense compound as a "wingmer" motif, having a wing-gap or gap-wing configuration, i.e. an X--Y or Y--Z configuration as described above for the gapmer configuration. Thus, wingmer configurations include, but are not limited to, for example 5-10, 8-4, 4-12, 12-4, 3-14, 16-2, 18-1, 10-3, 2-10, 1-10, 8-2, 2-13, or 5-13.

[0562] In certain embodiments, antisense compounds targeted to a DMPK nucleic acid possess a 5-10-5 gapmer motif. In certain embodiments, antisense compounds targeted to a DMPK nucleic acid possess a 5-7-5 gapmer motif. In certain embodiments, antisense compounds targeted to a DMPK nucleic acid possess a 3-10-3 gapmer motif. In certain embodiments, antisense compounds targeted to a DMPK nucleic acid possess a 4-8-4 gapmer motif.

[0563] In certain embodiments, an antisense compound targeted to a DMPK nucleic acid has a gap-widened motif.

[0564] In certain embodiments, antisense compounds of any of these gapmer or wingmer motifs contain at least 8, at least 9, at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, or at least 19, contiguous nucleobases of the nucleobase sequence of any of the exemplary antisense compounds described herein (e.g., at least 8 contiguous nucleobases of a nucleobase sequence recited in any one of SEQ ID NOs: 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, or 33-874.

[0565] In certain embodiments, the present invention provides oligomeric compounds comprising oligonucleotides. In certain embodiments, such oligonucleotides comprise one or more chemical modification. In certain embodiments, chemically modified oligonucleotides comprise one or more modified sugars. In certain embodiments, chemically modified oligonucleotides comprise one or more modified nucleobases. In certain embodiments, chemically modified oligonucleotides comprise one or more modified internucleoside linkages. In certain embodiments, the chemically modifications (sugar modifications, nucleobase modifications, and/or linkage modifications) define a pattern or motif. In certain embodiments, the patterns of chemical modifications of sugar moieties, internucleoside linkages, and nucleobases are each independent of one another. Thus, an oligonucleotide may be described by its sugar modification motif, internucleoside linkage motif and/or nucleobase modification motif (as used herein, nucleobase modification motif describes the chemical modifications to the nucleobases independent of the sequence of nucleobases).

[0566] Certain Sugar Motifs

[0567] In certain embodiments, oligonucleotides comprise one or more type of modified sugar moieties and/or naturally occurring sugar moieties arranged along an oligonucleotide or region thereof in a defined pattern or sugar modification motif. Such motifs may include any of the sugar modifications discussed herein and/or other known sugar modifications.

[0568] In certain embodiments, the oligonucleotides comprise or consist of a region having a gapmer sugar modification motif, which comprises two external regions or "wings" and an internal region or "gap." The three regions of a gapmer motif (the 5'-wing, the gap, and the 3'-wing) form a contiguous sequence of nucleosides wherein at least some of the sugar moieties of the nucleosides of each of the wings differ from at least some of the sugar moieties of the nucleosides of the gap. Specifically, at least the sugar moieties of the nucleosides of each wing that are closest to the gap (the 3'-most nucleoside of the 5'-wing and the 5'-most nucleoside of the 3'-wing) differ from the sugar moiety of the neighboring gap nucleosides, thus defining the boundary between the wings and the gap. In certain embodiments, the sugar moieties within the gap are the same as one another. In certain embodiments, the gap includes one or more nucleoside having a sugar moiety that differs from the sugar moiety of one or more other nucleosides of the gap. In certain embodiments, the sugar modification motifs of the two wings are the same as one another (symmetric gapmer). In certain embodiments, the sugar modification motifs of the 5'-wing differs from the sugar modification motif of the 3'-wing (asymmetric gapmer).

[0569] Certain 5'-Wings

[0570] In certain embodiments, the 5'-wing of a gapmer consists of 1 to 5 linked nucleosides. In certain embodiments, the 5'-wing of a gapmer consists of 2 to 5 linked nucleosides. In certain embodiments, the 5'-wing of a gapmer consists of 3 to 5 linked nucleosides. In certain embodiments, the 5'-wing of a gapmer consists of 4 or 5 linked nucleosides. In certain embodiments, the 5'-wing of a gapmer consists of 1 to 4 linked nucleosides. In certain embodiments, the 5'-wing of a gapmer consists of 1 to 3 linked nucleosides. In certain embodiments, the 5'-wing of a gapmer consists of 1 or 2 linked nucleosides. In certain embodiments, the 5'-wing of a gapmer consists of 2 to 4 linked nucleosides. In certain embodiments, the 5'-wing of a gapmer consists of 2 or 3 linked nucleosides. In certain embodiments, the 5'-wing of a gapmer consists of 3 or 4 linked nucleosides. In certain embodiments, the 5'-wing of a gapmer consists of 1 nucleoside. In certain embodiments, the 5'-wing of a gapmer consists of 2 linked nucleosides. In certain embodiments, the 5'-wing of a gapmer consists of 3 linked nucleosides. In certain embodiments, the 5'-wing of a gapmer consists of 4 linked nucleosides. In certain embodiments, the 5'-wing of a gapmer consists of 5 linked nucleosides.

[0571] In certain embodiments, the 5'-wing of a gapmer comprises at least one bicyclic nucleoside. In certain embodiments, the 5'-wing of a gapmer comprises at least two bicyclic nucleosides. In certain embodiments, the 5'-wing of a gapmer comprises at least three bicyclic nucleosides. In certain embodiments, the 5'-wing of a gapmer comprises at least four bicyclic nucleosides. In certain embodiments, the 5'-wing of a gapmer comprises at least one constrained ethyl nucleoside. In certain embodiments, the 5'-wing of a gapmer comprises at least one LNA nucleoside. In certain embodiments, each nucleoside of the 5'-wing of a gapmer is a bicyclic nucleoside. In certain embodiments, each nucleoside of the 5'-wing of a gapmer is a constrained ethyl nucleoside. In certain embodiments, each nucleoside of the 5'-wing of a gapmer is a LNA nucleoside.

[0572] In certain embodiments, the 5'-wing of a gapmer comprises at least one non-bicyclic modified nucleoside. In certain embodiments, the 5'-wing of a gapmer comprises at least one 2'-substituted nucleoside. In certain embodiments, the 5'-wing of a gapmer comprises at least one 2'-MOE nucleoside. In certain embodiments, the 5'-wing of a gapmer comprises at least one 2'-OMe nucleoside. In certain embodiments, each nucleoside of the 5'-wing of a gapmer is a non-bicyclic modified nucleoside. In certain embodiments, each nucleoside of the 5'-wing of a gapmer is a 2'-substituted nucleoside. In certain embodiments, each nucleoside of the 5'-wing of a gapmer is a 2'-MOE nucleoside. In certain embodiments, each nucleoside of the 5'-wing of a gapmer is a 2'-OMe nucleoside.

[0573] In certain embodiments, the 5'-wing of a gapmer comprises at least one bicyclic nucleoside and at least one non-bicyclic modified nucleoside. In certain embodiments, the 5'-wing of a gapmer comprises at least one bicyclic nucleoside and at least one 2'-substituted nucleoside. In certain embodiments, the 5'-wing of a gapmer comprises at least one bicyclic nucleoside and at least one 2'-MOE nucleoside. In certain embodiments, the 5'-wing of a gapmer comprises at least one bicyclic nucleoside and at least one 2'-OMe nucleoside. In certain embodiments, the 5'-wing of a gapmer comprises at least one bicyclic nucleoside and at least one 2'-deoxynucleoside.

[0574] In certain embodiments, the 5'-wing of a gapmer comprises at least one constrained ethyl nucleoside and at least one non-bicyclic modified nucleoside. In certain embodiments, the 5'-wing of a gapmer comprises at least one constrained ethyl nucleoside and at least one 2'-substituted nucleoside. In certain embodiments, the 5'-wing of a gapmer comprises at least one constrained ethyl nucleoside and at least one 2'-MOE nucleoside. In certain embodiments, the 5'-wing of a gapmer comprises at least one constrained ethyl nucleoside and at least one 2'-OMe nucleoside. In certain embodiments, the 5'-wing of a gapmer comprises at least one constrained ethyl nucleoside and at least one 2'-deoxynucleoside.

[0575] In certain embodiments, the 5'-wing of a gapmer comprises at least one LNA nucleoside and at least one non-bicyclic modified nucleoside. In certain embodiments, the 5'-wing of a gapmer comprises at least one LNA nucleoside and at least one 2'-substituted nucleoside. In certain embodiments, the 5'-wing of a gapmer comprises at least one LNA nucleoside and at least one 2'-MOE nucleoside. In certain embodiments, the 5'-wing of a gapmer comprises at least one LNA nucleoside and at least one 2'-OMe nucleoside. In certain embodiments, the 5'-wing of a gapmer comprises at least one LNA nucleoside and at least one 2'-deoxynucleoside.

[0576] In certain embodiments, the 5'-wing of a gapmer comprises three constrained ethyl nucleosides. In certain embodiments, the 5'-wing of a gapmer comprises two bicyclic nucleosides and two non bicyclic modified nucleosides. In certain embodiments, the 5'-wing of a gapmer comprises two constrained ethyl nucleosides and two 2'-MOE nucleosides. In certain embodiments, the 5'-wing of a gapmer comprises two bicyclic nucleosides and two non bicyclic modified nucleosides. In certain embodiments, the 5'-wing of a gapmer comprises two constrained ethyl nucleosides and two 2'-MOE nucleosides. In certain embodiments, the 5'-wing of a gapmer comprises two constrained ethyl nucleosides and three 2'-MOE nucleosides.

[0577] In certain embodiments, the 5'-wing of a gapmer comprises three LNA nucleosides. In certain embodiments, the 5'-wing of a gapmer comprises two LNAnucleosides and two non bicyclic modified nucleosides. In certain embodiments, the 5'-wing of a gapmer comprises two LNA nucleosides and two 2'-MOE nucleosides. In certain embodiments, the 5'-wing of a gapmer comprises two LNA and two non bicyclic modified nucleosides. In certain embodiments, the 5'-wing of a gapmer comprises two LNA nucleosides and two 2'-MOE nucleosides. In certain embodiments, the 5'-wing of a gapmer comprises two LNA nucleosides and three 2'-MOE nucleosides.

[0578] In certain embodiments, the 5'-wing of a gapmer comprises three constrained ethyl nucleosides. In certain embodiments, the 5'-wing of a gapmer comprises two bicyclic nucleosides and two non bicyclic modified nucleosides. In certain embodiments, the 5'-wing of a gapmer comprises two constrained ethyl nucleosides and two 2'-OMe nucleosides. In certain embodiments, the 5'-wing of a gapmer comprises two bicyclic nucleosides and two non bicyclic modified nucleosides. In certain embodiments, the 5'-wing of a gapmer comprises two constrained ethyl nucleosides and two 2'-OMe nucleosides. In certain embodiments, the 5'-wing of a gapmer comprises two constrained ethyl nucleosides and three 2'-OMe nucleosides.

[0579] In certain embodiments, the 5'-wing of a gapmer comprises three LNA nucleosides. In certain embodiments, the 5'-wing of a gapmer comprises two LNAnucleosides and two non bicyclic modified nucleosides. In certain embodiments, the 5'-wing of a gapmer comprises two LNA nucleosides and two 2'-OMe nucleosides. In certain embodiments, the 5'-wing of a gapmer comprises two LNA and two non bicyclic modified nucleosides. In certain embodiments, the 5'-wing of a gapmer comprises two LNA nucleosides and two 2'-OMe nucleosides. In certain embodiments, the 5'-wing of a gapmer comprises two LNA nucleosides and three 2'-OMe nucleosides.

[0580] In certain embodiments, the 5'-wing of a gapmer has an AABB motif, wherein each A is selected from among a 2'-MOE nucleoside and a 2'OMe nucleoside. In certain embodiments, the 5'-wing of a gapmer has an AABB motif, wherein each B is selected from among a cEt, LNA, .alpha.-L-LNA, ENA and 2'-thio LNA nucleoside. In certain embodiments, the 5'-wing of a gapmer has an AABB motif, wherein each A represents a 2'-MOE nucleoside and each B represents a constrained ethyl nucleoside.

[0581] In certain embodiments, the 5'-wing of a gapmer has an AAABB motif, wherein each A is selected from among a 2'-MOE nucleoside and a 2'OMe nucleoside. In certain embodiments, the 5'-wing of a gapmer has an AABB motif, wherein each B is selected from among a cEt, LNA, .alpha.-L-LNA, ENA and 2'-thio LNA nucleoside. In certain embodiments, the 5'-wing of a gapmer has an AABB motif, wherein each A represents a 2'-MOE nucleoside and each B represents a constrained ethyl nucleoside.

Certain 3'-Wings

[0582] In certain embodiments, the 3'-wing of a gapmer consists of 1 to 5 linked nucleosides. In certain embodiments, the 3'-wing of a gapmer consists of 2 to 5 linked nucleosides. In certain embodiments, the 3'-wing of a gapmer consists of 3 to 5 linked nucleosides. In certain embodiments, the 3'-wing of a gapmer consists of 4 or 5 linked nucleosides. In certain embodiments, the 3'-wing of a gapmer consists of 1 to 4 linked nucleosides. In certain embodiments, the 3'-wing of a gapmer consists of 1 to 3 linked nucleosides. In certain embodiments, the 3'-wing of a gapmer consists of 1 or 2 linked nucleosides. In certain embodiments, the 3'-wing of a gapmer consists of 2 to 4 linked nucleosides. In certain embodiments, the 3'-wing of a gapmer consists of 2 or 3 linked nucleosides. In certain embodiments, the 3'-wing of a gapmer consists of 3 or 4 linked nucleosides. In certain embodiments, the 3'-wing of a gapmer consists of 1 nucleoside. In certain embodiments, the 3'-wing of a gapmer consists of 2 linked nucleosides. In certain embodiments, the 3'-wing of a gapmer consists of 3 linked nucleosides. In certain embodiments, the 3'-wing of a gapmer consists of 4 linked nucleosides. In certain embodiments, the 3'-wing of a gapmer consists of 5 linked nucleosides.

[0583] In certain embodiments, the 3'-wing of a gapmer comprises at least one bicyclic nucleoside. In certain embodiments, the 3'-wing of a gapmer comprises at least one constrained ethyl nucleoside. In certain embodiments, the 3'-wing of a gapmer comprises at least one LNA nucleoside. In certain embodiments, each nucleoside of the 3'-wing of a gapmer is a bicyclic nucleoside. In certain embodiments, each nucleoside of the 3'-wing of a gapmer is a constrained ethyl nucleoside. In certain embodiments, each nucleoside of the 3'-wing of a gapmer is a LNA nucleoside.

[0584] In certain embodiments, the 3'-wing of a gapmer comprises at least one non-bicyclic modified nucleoside. In certain embodiments, the 3'-wing of a gapmer comprises at least two non-bicyclic modified nucleosides. In certain embodiments, the 3'-wing of a gapmer comprises at least three non-bicyclic modified nucleosides. In certain embodiments, the 3'-wing of a gapmer comprises at least four non-bicyclic modified nucleosides. In certain embodiments, the 3'-wing of a gapmer comprises at least one 2'-substituted nucleoside. In certain embodiments, the 3'-wing of a gapmer comprises at least one 2'-MOE nucleoside. In certain embodiments, the 3'-wing of a gapmer comprises at least one 2'-OMe nucleoside. In certain embodiments, each nucleoside of the 3'-wing of a gapmer is a non-bicyclic modified nucleoside. In certain embodiments, each nucleoside of the 3'-wing of a gapmer is a 2'-substituted nucleoside. In certain embodiments, each nucleoside of the 3'-wing of a gapmer is a 2'-MOE nucleoside. In certain embodiments, each nucleoside of the 3'-wing of a gapmer is a 2'-OMe nucleoside.

[0585] In certain embodiments, the 3'-wing of a gapmer comprises at least one bicyclic nucleoside and at least one non-bicyclic modified nucleoside. In certain embodiments, the 3'-wing of a gapmer comprises at least one bicyclic nucleoside and at least one 2'-substituted nucleoside. In certain embodiments, the 3'-wing of a gapmer comprises at least one bicyclic nucleoside and at least one 2'-MOE nucleoside. In certain embodiments, the 3'-wing of a gapmer comprises at least one bicyclic nucleoside and at least one 2'-OMe nucleoside. In certain embodiments, the 3'-wing of a gapmer comprises at least one bicyclic nucleoside and at least one 2'-deoxynucleoside.

[0586] In certain embodiments, the 3'-wing of a gapmer comprises at least one constrained ethyl nucleoside and at least one non-bicyclic modified nucleoside. In certain embodiments, the 3'-wing of a gapmer comprises at least one constrained ethyl nucleoside and at least one 2'-substituted nucleoside. In certain embodiments, the 3'-wing of a gapmer comprises at least one constrained ethyl nucleoside and at least one 2'-MOE nucleoside. In certain embodiments, the 3'-wing of a gapmer comprises at least one constrained ethyl nucleoside and at least one 2'-OMe nucleoside. In certain embodiments, the 3'-wing of a gapmer comprises at least one constrained ethyl nucleoside and at least one 2'-deoxynucleoside.

[0587] In certain embodiments, the 3'-wing of a gapmer comprises at least one LNA nucleoside and at least one non-bicyclic modified nucleoside. In certain embodiments, the 3'-wing of a gapmer comprises at least one LNA nucleoside and at least one 2'-substituted nucleoside. In certain embodiments, the 3'-wing of a gapmer comprises at least one LNA nucleoside and at least one 2'-MOE nucleoside. In certain embodiments, the 3'-wing of a gapmer comprises at least one LNA nucleoside and at least one 2'-OMe nucleoside. In certain embodiments, the 3'-wing of a gapmer comprises at least one LNA nucleoside and at least one 2'-deoxynucleoside.

[0588] In certain embodiments, the 3'-wing of a gapmer comprises three constrained ethyl nucleosides. In certain embodiments, the 3'-wing of a gapmer comprises two bicyclic nucleosides and two non bicyclic modified nucleosides. In certain embodiments, the 3'-wing of a gapmer comprises two constrained ethyl nucleosides and two 2'-MOE nucleosides. In certain embodiments, the 3'-wing of a gapmer comprises two bicyclic nucleosides and two non bicyclic modified nucleosides. In certain embodiments, the 3'-wing of a gapmer comprises two constrained ethyl nucleosides and two 2'-MOE nucleosides. In certain embodiments, the 5'-wing of a gapmer comprises two constrained ethyl nucleosides and three 2'-MOE nucleosides.

[0589] In certain embodiments, the 3'-wing of a gapmer comprises three LNA nucleosides. In certain embodiments, the 3'-wing of a gapmer comprises two LNAnucleosides and two non bicyclic modified nucleosides. In certain embodiments, the 3'-wing of a gapmer comprises two LNA nucleosides and two 2'-MOE nucleosides. In certain embodiments, the 3'-wing of a gapmer comprises two LNA and two non bicyclic modified nucleosides. In certain embodiments, the 3'-wing of a gapmer comprises two LNA nucleosides and two 2'-MOE nucleosides. In certain embodiments, the 3'-wing of a gapmer comprises two LNA nucleosides and three 2'-MOE nucleosides.

[0590] In certain embodiments, the 3'-wing of a gapmer comprises three constrained ethyl nucleosides. In certain embodiments, the 3'-wing of a gapmer comprises two bicyclic nucleosides and two non bicyclic modified nucleosides. In certain embodiments, the 3'-wing of a gapmer comprises two constrained ethyl nucleosides and two 2'-OMe nucleosides. In certain embodiments, the 3'-wing of a gapmer comprises two bicyclic nucleosides and two non bicyclic modified nucleosides. In certain embodiments, the 3'-wing of a gapmer comprises two constrained ethyl nucleosides and two 2'-OMe nucleosides. In certain embodiments, the 3'-wing of a gapmer comprises two constrained ethyl nucleosides and three 2'-OMe nucleosides.

[0591] In certain embodiments, the 3'-wing of a gapmer comprises three LNA nucleosides. In certain embodiments, the 3'-wing of a gapmer comprises two LNAnucleosides and two non bicyclic modified nucleosides. In certain embodiments, the 3'-wing of a gapmer comprises two LNA nucleosides and two 2'-OMe nucleosides. In certain embodiments, the 3'-wing of a gapmer comprises two LNA and two non bicyclic modified nucleosides. In certain embodiments, the 3'-wing of a gapmer comprises two LNA nucleosides and two 2'-OMe nucleosides. In certain embodiments, the 3'-wing of a gapmer comprises two LNA nucleosides and three 2'-OMe nucleosides.

[0592] In certain embodiments, the 3'-wing of a gapmer has a BBAA motif, wherein each A is selected from among a 2'-MOE nucleoside and a 2'OMe nucleoside. In certain embodiments, the 3'-wing of a gapmer has an BBAA motif, wherein each B is selected from among a cEt, LNA, .alpha.-L-LNA, ENA and 2'-thio LNA nucleoside. In certain embodiments, the 3'-wing of a gapmer has a BBAA motif, wherein each A represents a 2'-MOE nucleoside and each B represents a constrained ethyl nucleoside.

[0593] In certain embodiments, the 3'-wing of a gapmer has a BBAAA motif, wherein each A is selected from among a 2'-MOE nucleoside and a 2'OMe nucleoside. In certain embodiments, the 3'-wing of a gapmer has a BBAA motif, wherein each B is selected from among a cEt, LNA, .alpha.-L-LNA, ENA and 2'-thio LNA nucleoside. In certain embodiments, the 3'-wing of a gapmer has a BBAA motif, wherein each A represents a 2'-MOE nucleoside and each B represents a constrained ethyl nucleoside.

Compositions and Methods for Formulating Pharmaceutical Compositions

[0594] Antisense oligonucleotides can be admixed with pharmaceutically acceptable active or inert substance for the preparation of pharmaceutical compositions or formulations. Compositions and methods for the formulation of pharmaceutical compositions are dependent upon a number of criteria, including, but not limited to, route of administration, extent of disease, or dose to be administered.

[0595] Antisense compound targeted to a DMPK nucleic acid can be utilized in pharmaceutical compositions by combining the antisense compound with a suitable pharmaceutically acceptable diluent or carrier. A pharmaceutically acceptable diluent includes phosphate-buffered saline (PBS). PBS is a diluent suitable for use in compositions to be delivered parenterally. Accordingly, in one embodiment, employed in the methods described herein is a pharmaceutical composition comprising an antisense compound targeted to a DMPK nucleic acid and a pharmaceutically acceptable diluent. In certain embodiments, the pharmaceutically acceptable diluent is PBS. In certain embodiments, the antisense compound is an antisense oligonucleotide.

[0596] Pharmaceutical compositions comprising antisense compounds encompass any pharmaceutically acceptable salts, esters, or salts of such esters, or any other oligonucleotide which, upon administration to an animal, including a human, is capable of providing (directly or indirectly) the biologically active metabolite or residue thereof. Accordingly, for example, the disclosure is also drawn to pharmaceutically acceptable salts of antisense compounds, prodrugs, pharmaceutically acceptable salts of such prodrugs, and other bioequivalents. Suitable pharmaceutically acceptable salts include, but are not limited to, sodium and potassium salts.

[0597] A prodrug can include the incorporation of additional nucleosides at one or both ends of an antisense compound which are cleaved by endogenous nucleases within the body, to form the active antisense compound.

Conjugated Antisense Compounds

[0598] Antisense compounds can be covalently linked to one or more moieties or conjugates which enhance the activity, cellular distribution or cellular uptake of the resulting antisense oligonucleotides. Typical conjugate groups include cholesterol moieties and lipid moieties. Additional conjugate groups include carbohydrates, phospholipids, biotin, phenazine, folate, phenanthridine, anthraquinone, acridine, fluoresceins, rhodamines, coumarins, and dyes.

[0599] Antisense compounds can also be modified to have one or more stabilizing groups that are generally attached to one or both termini of antisense compounds to enhance properties such as, for example, nuclease stability. Included in stabilizing groups are cap structures. These terminal modifications protect the antisense compound having terminal nucleic acid from exonuclease degradation, and can help in delivery and/or localization within a cell. The cap can be present at the 5'-terminus (5'-cap), or at the 3'-terminus (3'-cap), or can be present on both termini. Cap structures are well known in the art and include, for example, inverted deoxy abasic caps. Further 3' and 5'-stabilizing groups that can be used to cap one or both ends of an antisense compound to impart nuclease stability include those disclosed in WO 03/004602 published on Jan. 16, 2003.

Cell Culture and Antisense Compounds Treatment

[0600] The effects of antisense compounds on the level, activity or expression of DMPK nucleic acids can be tested in vitro in a variety of cell types. Cell types used for such analyses are available from commercial vendors (e.g. American Type Culture Collection, Manassas, Va.; Zen-Bio, Inc., Research Triangle Park, N.C.; Clonetics Corporation, Walkersville, Md.) and cells are cultured according to the vendor's instructions using commercially available reagents (e.g. Invitrogen Life Technologies, Carlsbad, Calif.). Illustrative cell types include, but are not limited to, HepG2 cells, Hep3B cells, primary hepatocytes, A549 cells, GM04281 fibroblasts and LLC-MK2 cells.

In Vitro Testing of Antisense Oligonucleotides

[0601] Described herein are methods for treatment of cells with antisense oligonucleotides, which can be modified appropriately for treatment with other antisense compounds.

[0602] In general, cells are treated with antisense oligonucleotides when the cells reach approximately 60-80% confluence in culture.

[0603] One reagent commonly used to introduce antisense oligonucleotides into cultured cells includes the cationic lipid transfection reagent LIPOFECTIN.RTM. (Invitrogen, Carlsbad, Calif.). Antisense oligonucleotides are mixed with LIPOFECTIN.RTM. in OPTI-MEM.RTM. 1 (Invitrogen, Carlsbad, Calif.) to achieve the desired final concentration of antisense oligonucleotide and a LIPOFECTIN.RTM. concentration that typically ranges 2 to 12 ug/mL per 100 nM antisense oligonucleotide.

[0604] Another reagent used to introduce antisense oligonucleotides into cultured cells includes LIPOFECTAMINE 2000.RTM. (Invitrogen, Carlsbad, Calif.). Antisense oligonucleotide is mixed with LIPOFECTAMINE 2000.RTM. in OPTI-MEM.RTM. 1 reduced serum medium (Invitrogen, Carlsbad, Calif.) to achieve the desired concentration of antisense oligonucleotide and a LIPOFECTAMINE.RTM. concentration that typically ranges 2 to 12 ug/mL per 100 nM antisense oligonucleotide.

[0605] Another reagent used to introduce antisense oligonucleotides into cultured cells includes Cytofectin.RTM. (Invitrogen, Carlsbad, Calif.). Antisense oligonucleotide is mixed with Cytofectin.RTM. in OPTI-MEM.RTM. 1 reduced serum medium (Invitrogen, Carlsbad, Calif.) to achieve the desired concentration of antisense oligonucleotide and a Cytofectin.RTM. concentration that typically ranges 2 to 12 ug/mL per 100 nM antisense oligonucleotide.

[0606] Another technique used to introduce antisense oligonucleotides into cultured cells includes electroporation.

[0607] Cells are treated with antisense oligonucleotides by routine methods. Cells are typically harvested 16-24 hours after antisense oligonucleotide treatment, at which time RNA or protein levels of target nucleic acids are measured by methods known in the art and described herein. In general, when treatments are performed in multiple replicates, the data are presented as the average of the replicate treatments.

[0608] The concentration of antisense oligonucleotide used varies from cell line to cell line. Methods to determine the optimal antisense oligonucleotide concentration for a particular cell line are well known in the art. Antisense oligonucleotides are typically used at concentrations ranging from 1 nM to 300 nM when transfected with LIPOFECTAMINE2000.RTM., Lipofectin or Cytofectin. Antisense oligonucleotides are used at higher concentrations ranging from 625 to 20,000 nM when transfected using electroporation.

RNA Isolation

[0609] RNA analysis can be performed on total cellular RNA or poly(A)+mRNA. Methods of RNA isolation are well known in the art. RNA is prepared using methods well known in the art, for example, using the TRIZOL.RTM. Reagent (Invitrogen, Carlsbad, Calif.) according to the manufacturer's recommended protocols.

Analysis of Inhibition of Target Levels or Expression

[0610] Inhibition of levels or expression of a DMPK nucleic acid can be assayed in a variety of ways known in the art. For example, target nucleic acid levels can be quantitated by, e.g., Northern blot analysis, competitive polymerase chain reaction (PCR), or quantitaive real-time PCR. RNA analysis can be performed on total cellular RNA or poly(A)+mRNA. Methods of RNA isolation are well known in the art. Northern blot analysis is also routine in the art. Quantitative real-time PCR can be conveniently accomplished using the commercially available ABI PRISM.RTM. 7600, 7700, or 7900 Sequence Detection System, available from PE-Applied Biosystems, Foster City, Calif. and used according to manufacturer's instructions.

Quantitative Real-Time PCR Analysis of Target RNA Levels

[0611] Quantitation of target RNA levels can be accomplished by quantitative real-time PCR using the ABI PRISM.RTM. 7600, 7700, or 7900 Sequence Detection System (PE-Applied Biosystems, Foster City, Calif.) according to manufacturer's instructions. Methods of quantitative real-time PCR are well known in the art.

[0612] Prior to real-time PCR, the isolated RNA is subjected to a reverse transcriptase (RT) reaction, which produces complementary DNA (cDNA) that is then used as the substrate for the real-time PCR amplification. The RT and real-time PCR reactions are performed sequentially in the same sample well. RT and real-time PCR reagents are obtained from Invitrogen (Carlsbad, Calif.). RT, real-time-PCR reactions are carried out by methods well known to those skilled in the art.

[0613] Gene (or RNA) target quantities obtained by real time PCR are normalized using either the expression level of a gene whose expression is constant, such as cyclophilin A, or by quantifying total RNA using RIBOGREEN.RTM. (Invitrogen, Inc. Carlsbad, Calif.). Cyclophilin A expression is quantified by real time PCR, by being run simultaneously with the target, multiplexing, or separately. Total RNA is quantified using RIBOGREEN.RTM. RNA quantification reagent (Invitrogen, Inc. Eugene, Oreg.). Methods of RNA quantification by RIBOGREEN.RTM. are taught in Jones, L. J., et al, (Analytical Biochemistry, 1998, 265, 368-374). A CYTOFLUOR.RTM. 4000 instrument (PE Applied Biosystems) is used to measure RIBOGREEN.RTM. fluorescence.

[0614] Probes and primers are designed to hybridize to a DMPK nucleic acid. Methods for designing real-time PCR probes and primers are well known in the art, and can include the use of software such as PRIMER EXPRESS.RTM. Software (Applied Biosystems, Foster City, Calif.).

Analysis of Protein Levels

[0615] Antisense inhibition of DMPK nucleic acids can be assessed by measuring DMPK protein levels. Protein levels of DMPK can be evaluated or quantitated in a variety of ways well known in the art, such as immunoprecipitation, Western blot analysis (immunoblotting), enzyme-linked immunosorbent assay (ELISA), quantitative protein assays, protein activity assays (for example, caspase activity assays), immunohistochemistry, immunocytochemistry or fluorescence-activated cell sorting (FACS). Antibodies directed to a target can be identified and obtained from a variety of sources, such as the MSRS catalog of antibodies (Aerie Corporation, Birmingham, Mich.), or can be prepared via conventional monoclonal or polyclonal antibody generation methods well known in the art.

In Vivo Testing of Antisense Compounds

[0616] Antisense compounds, for example, antisense oligonucleotides, are tested in animals to assess their ability to inhibit expression of DMPK and produce phenotypic changes. Testing can be performed in normal animals, or in experimental disease models, for example, the HSA.sup.LR mouse model of myotonic dystrophy (DM1).

[0617] The HSA.sup.LR mouse model is an established model for DM1 (Mankodi, A. et al. Science. 289: 1769, 2000). The mice carry a human skeletal actin (hACTA1) transgene with 220 CTG repeats inserted in the 3' UTR of the gene. The hACTA1-CUG.sup.exp transcript accumulates in nuclear foci in skeletal muscles and results in myotonia similar to that in human DM1 (Mankodi, A. et al. Mol. Cell 10: 35, 2002; Lin, X. et al. Hum. Mol. Genet. 15: 2087, 2006). Hence, it is expected that amelioration of DM1 symptoms in the HSA.sup.LR mouse by antisense inhibition of the hACTA1 transgene would predict amelioration of similar symptoms in human patients by antisense inhibition of the DMPK transcript.

[0618] Expression of CUG.sup.exp RNA in mice causes extensive remodeling of the muscle transcriptome, much of which is reproduced by ablation of MBNL1. Hence, it is expected that normalization of the transcriptome in HSA.sup.LR mice would predict normalization of the human transcriptome in DM1 patients by antisense inhibition of the DMPK transcript.

[0619] For administration to animals, antisense oligonucleotides are formulated in a pharmaceutically acceptable diluent, such as phosphate-buffered saline. Administration includes parenteral routes of administration. Following a period of treatment with antisense oligonucleotides, RNA is isolated from tissue and changes in DMPK nucleic acid expression are measured. Changes in DMPK protein levels are also measured.

Splicing

[0620] Myotonic dystrophy (DM1) is caused by CTG repeat expansions in the 3' untranslated region of the DMPK gene (Brook, J. D. et al. Cell. 68: 799, 1992). This mutation leads to RNA dominance, a process in which expression of RNA containing an expanded CUG repeat (CUGexp) induces cell dysfunction (Osborne R J and Thornton C A., Human Molecular Genetics., 2006, 15(2): R162-R169). Such CUGexp are retained in the nuclear foci of skeletal muscles (Davis, B. M. et al. Proc. Natl. Acad. Sci. U.S.A. 94:7388, 1997). The accumulation of CUGexp in the nuclear foci leads to the sequestration of poly(CUG)-binding proteins, such as, Muscleblind-like 1 (MBLN1) (Miller, J. W. et al. EMBO J. 19: 4439, 2000). MBLN1 is a splicing factor and regulates the splicing of genes such as Serca1, CIC-1, Titin, and Zasp. Therefore, sequestration of MBLN1 by CUGexp triggers misregulated alternative splicing of the exons of genes that MBLN1 normally controls (Lin, X. et al. Hum. Mol. Genet. 15: 2087, 2006). Correction of alternative splicing in an animal displaying such disregulation, such as, for example, in a DM1 patient and the HSALR mouse model, is a useful indicator for the efficacy of a treatment, including treatment with an antisense oligonucleotide.

Certain Antisense Mechanisms

[0621] Myotonic dystrophy (DM1) is caused by CTG repeat expansions in the 3' untranslated region of the DMPK gene. In certain embodiments, expansions in the 3' untranslated region of the DMPK gene results in the transcription of RNA containing an expanded CUG repeat, and RNA containing an expanded CUG repeat (CUGexp) is retained in the nuclear foci of skeletal muscles. In certain instances, the cellular machinery responsible for exporting mRNA from the nucleus into the cytoplasm does not export RNA containing an expanded CUG repeat from the nucleus or does so less efficiently. In certain embodiments, cells do not export DMPK CUGexp mRNA from the nucleus or such export is reduced. Accordingly, in certain embodiments, DMPK CUGexp mRNA accumulates in the nucleus. In certain embodiments, more copies of DMPK CUGexp mRNA are present in the nucleus of a cell than are copies of wild-type DMPK mRNA, which is exported normally. In such embodiments, antisense compounds that reduce target in the nucleus will preferentially reduce mutant DMPK CUGexp mRNA relative to wild type DMPK mRNA, due to their relative abundences in the nucleus, even if the antisense compound does not otherwise distinguish between mutant and wild type. Since RNase H dependent antisense compounds are active in the nucleus, such compounds are particularly well suited for such use.

[0622] In certain instances, wild-type DMPK pre-mRNA and mutant CUGexp DMPK pre-mRNA are expected to be processed into mRNA at similar rate. Accordingly, approximately the same amount of wild-type DMPK pre-mRNA and mutant CUGexp DMPK pre-mRNA are expected to be present in the nucleus of a cell. However, after proceesing, wild type DMPK mRNA is exported from the nucleus relatively quickly, and mutant CUGexp DMPK mRNA is exported slowly or not at all. In certan such embodiments, mutant CUGexp DMPK mRNA accumulates in the nucleus in greater amounts than wild-type DMPK mRNA. In certain such embodiments, an antisense oligonucleotide targeted to the mRNA, will preferentially reduce the expression of the mutant CUGexp DMPK mRNA compared to the wild-type DMPK mRNA because more copies of the mutant CUGexp DMPK mRNA are present in the nucleus of the cell. In certain embodiments, antisense compounds targeted to pre-mRNA and not mRNA (e.g., targeting an intron) are not expected to preferentially reduce mutant DMPK relative to wild type, because the nuclear abundance of the two pre-mRNAs is likely to be similar. In certain embodiments, antisense compounds described herein are not targeted to introns of DMPK pre-mRNA. In certain embodiments, antisense compounds described herein are targeted to exons or exon-exon junctions present in DMPK mRNA. In certain embodiments, use of an antisense oligonucleotide to target the mRNA is therefore preferred because an antisense oligonucleotide having one or more features described herein (i) has activity in the nucleus of a cell and (2) will preferentially reduce mutant CUGexp DMPK mRNA compared to wild-type DMPK mRNA.

Certain Biomarkers

[0623] DM1 severity in mouse models is determined, at least in part, by the level of CUG.sup.exp transcript accumulation in the nucleus or nuclear foci. A useful physiological marker for DM1 severity is the development of high-frequency runs of involuntary action potentials (myotonia).

Certain Indications

[0624] In certain embodiments, provided herein are methods of treating an individual comprising administering one or more pharmaceutical compositions as described herein. In certain embodiments, the individual has type 1 myotonic dystrophy (DM1).

[0625] Accordingly, provided herein are methods for ameliorating a symptom associated with type 1 myotonic dystrophy in a subject in need thereof. In certain embodiments, provided is a method for reducing the rate of onset of a symptom associated with type 1 myotonic dystrophy. In certain embodiments, provided is a method for reducing the severity of a symptom associated with type 1 myotonic dystrophy. In certain embodiments, symptoms associated with DM1 include muscle stiffness, myotonia, disabling distal weakness, weakness in face and jaw muscles, difficulty in swallowing, drooping of the eyelids (ptosis), weakness of neck muscles, weakness in arm and leg muscles, persistent muscle pain, hypersomnia, muscle wasting, dysphagia, respiratory insufficiency, irregular heartbeat, heart muscle damage, apathy, insulin resistance, and cataracts. In children, the symptoms may also be developmental delays, learning problems, language and speech issues, and personality development issues.

[0626] In certain embodiments, the methods comprise administering to an individual in need thereof a therapeutically effective amount of a compound targeted to a DMPK nucleic acid.

[0627] In certain embodiments, administration of an antisense compound targeted to a DMPK nucleic acid results in reduction of DMPK expression by at least about 15%, by at least about 20%, by at least about 25%, by at least about 30%, by at least about 35%, by at least about 40%, by at least about 45%, by at least about 50%, by at least about 55%, by at least about 60%, by least about 65%, by least about 70%, by least about 75%, by least about 80%, by at least about 85%, by at least about 90%, by at least about 95% or by at least about 99%, or a range defined by any two of these values.

[0628] In certain embodiments, pharmaceutical compositions comprising an antisense compound targeted to DMPK are used for the preparation of a medicament for treating a patient suffering or susceptible to type 1 myotonic dystrophy.

[0629] In certain embodiments, the methods described herein include administering a compound comprising a modified oligonucleotide having a contiguous nucleobases portion as described herein of a sequence recited in SEQ ID NOs: 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, or 33-874.

Administration

[0630] In certain embodiments, the compounds and compositions as described herein are administered parenterally.

[0631] In certain embodiments, parenteral administration is by infusion. Infusion can be chronic or continuous or short or intermittent. In certain embodiments, infused pharmaceutical agents are delivered with a pump. In certain embodiments, parenteral administration is by injection (e.g., bolus injection). The injection can be delivered with a syringe.

[0632] Parenteral administration includes subcutaneous administration, intravenous administration, intramuscular administration, intraarterial administration, intraperitoneal administration, or intracranial administration, e.g., intrathecal or intracerebroventricular administration. Administration can be continuous, or chronic, or short, or intermittent.

[0633] In certain embodiments, the administering is subcutaneous, intravenous, intracerebral, intracerebroventricular, intrathecal or another administration that results in a systemic effect of the oligonucleotide (systemic administration is characterized by a systemic effect, i.e., an effect in more than one tissue) or delivery to the CNS or to the CSF.

[0634] The duration of action as measured by inhibition of alpha 1 actin and reduction of myotonia in the HSA.sup.LR mouse model of DM1 is prolonged in muscle tissue including quadriceps, gastrocnemius, and the tibialis anterior (see Examples, below). Subcutaneous injections of antisense oligonucleotide for 4 weeks results in inhibition of alpha 1 actin by at least 70% in quadriceps, gastrocnemius, and the tibialis anterior in HSA.sup.LR mice for at least 11 weeks (77 days) after termination of dosing. Subcutaneous injections of antisense oligonucleotide for 4 weeks results in elimination of myotonia in quadriceps, gastrocnemius, and the tibialis anterior in HSA.sup.LR mice for at least 11 weeks (77 days) after termination of dosing.

[0635] In certain embodiments, delivery of a compound of composition, as described herein, results in at least 70% down-regulation of a target mRNA and/or target protein for at least 77 days. In certain embodiments, delivery of a compound or composition, as described herein, results in 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, or 100% down-regulation of a target mRNA and/or target protein for at least 30 days, at least 35 days, at least 40 days, at least 45 days, at least 50 days, at least 55 days, at least 60 days, at least 65 days, at least 70 days, at least 75 days, at least 76 days, at least 77 days, at least 78 days, at least 79 days, at least 80 days, at least 85 days, at least 90 days, at least 95 days, at least 100 days, at least 105 days, at least 110 days, at least 115 days, at least 120 days, at least 1 year.

[0636] In certain embodiments, an antisense oligonucleotide is delivered by injection or infusion once every 77 days. In certain embodiments, an antisense oligonucleotide is delivered by injection or infusion once every month, every two months, every three months, every 6 months, twice a year or once a year.

Certain Combination Therapies

[0637] In certain embodiments, a first agent comprising the modified oligonucleotide of the invention is co-administered with one or more secondary agents. In certain embodiments, such second agents are designed to treat the same type 1 myotonic dystrophy as the first agent described herein. In certain embodiments, such second agents are designed to treat a different disease, disorder, or condition as the first agent described herein. In certain embodiments, such second agents are designed to treat an undesired side effect of one or more pharmaceutical compositions as described herein. In certain embodiments, second agents are co-administered with the first agent to treat an undesired effect of the first agent. In certain embodiments, second agents are co-administered with the first agent to produce a combinational effect. In certain embodiments, second agents are co-administered with the first agent to produce a synergistic effect.

[0638] In certain embodiments, a first agent and one or more second agents are administered at the same time. In certain embodiments, the first agent and one or more second agents are administered at different times. In certain embodiments, the first agent and one or more second agents are prepared together in a single pharmaceutical formulation. In certain embodiments, the first agent and one or more second agents are prepared separately.

Certain Comparator Compounds

[0639] In certain embodiments, the compounds disclosed herein benefit from one or more improved in vitro and/or in vivo properties relative to an appropriate comparator compound.

[0640] In certain embodiments, ISIS 445569, a 5-10-5 MOE gapmer, having a sequence of (from 5' to 3') CGGAGCGGTTGTGAACTGGC (incorporated herein as SEQ ID NO: 24), wherein each internucleoside linkage is a phosphorothioate linkage, each cytosine is a 5-methylcytosine, and each of nucleosides 1-5 and 16-20 comprise a 2'-O-methoxyethyl moiety, which was previously described in WO 2012/012443, incorporated herein by reference, is a comparator compound.

[0641] ISIS 445569 is an appropriate representative comparator compound because ISIS 445569 demonstrates statistically significant reduction of human DMPK in vitro as measured using a plurality of primer probe sets (see e.g. Example 1 and Example 2 of WO 2012/012443). Additionally, ISIS 445569 demonstrates statistically significant dose-dependent inhibition of human DMPK in vitro in both human skeletal muscle cells and DM1 fibroblasts (see e.g. Example 4 and Example 5 of WO 2012/012443 and Example 28 of WO 2012/012467). ISIS 445569 also reduces human DMPK transcript expression in transgenic mice (Examples 23 and 24 of WO 2012/012443 and Examples 29 and 30 of WO 2012/012467). ISIS 445569 was a preferred human DMPK antisense compound in WO 2012/012443 and WO 2012/012467.

Certain Compounds

[0642] In certain embodiments, the compounds disclosed herein benefit from improved activity and/or improved tolerability relative to appropriate comparator compounds, such as ISIS 445569. For example, in certain embodiments, ISIS 598769, ISIS 598768, and/or ISIS 486178 have more activity and/or tolerability than appropriate comparator compounds, such as ISIS 445569.

[0643] In certain embodiments, the compounds disclosed herein are more potent than appropriate comparator compounds, such as ISIS 445569. For example, as provided in Example 10 (described herein), ISIS 598769 achieved an IC.sub.50 of 1.9 .mu.M, ISIS 598768 achieved an IC.sub.50 of 1.2 .mu.M, and ISIS 486178 achieved an IC.sub.50 of 0.7 .mu.M in a 6 point dose response curve (61.7 nM, 185.2 nM, 555.6 nM, 1666.7 nM, 5000.0 nM, and 15000.0 nM) in cultured in HepG2 cells when transfected using electroporation, whereas ISIS 445569 achieved an IC.sub.50 of 2.3 .mu.M. Thus, ISIS 598769, ISIS 598768, and ISIS 486178 are more potent than the comparator compound, ISIS 445569.

[0644] In certain embodiments, the compounds disclosed herein have greater activity than appropriate comparator compounds, such as ISIS 445569, at achieving dose-dependent inhibition of DMPK across multiple different muscle tissues. In another example, as provided in Example 16 (described herein), ISIS 598768 and ISIS 598769 achieved greater dose-dependent inhibition than the comparator compound ISIS 445569 across several different muscle tissues when administered subcutaneously to DMSXL transgenic mice twice a week for 4 weeks with 25 mg/kg/week, 50 mg/kg/wk, or 100 mg/kg/wk. In some muscle tissues, for example, in the tibialis anterior, both ISIS 598768 and ISIS 598769 achieved greater inhibition of DMPK at 25, 50 and 100 mg/kg/wk than ISIS 445569 achieved at 200 mg/kg/wk. In the quadriceps and gastrocnemius, both ISIS 598768 and ISIS 598769 achieved equal or greater inhibition of DMPK at 50 mg/kg/wk than ISIS 445569 achieved at 100 or 200 mg/kg/wk. Thus, ISIS 598768 and ISIS 598769 have greater activity than ISIS 445569 at achieving dose-dependent inhibition of DMPK across multiple different muscle tissues.

[0645] In certain embodiments, the compounds disclosed herein are more tolerable than appropriate comparator compounds, such as ISIS 445569, when administered to CD-1 mice. In another example, as provided in Example 17 (described herein), ISIS 598769, ISIS 598768, and ISIS 486178 exhibited more favorable tolerability markers than ISIS 445569 when administered to CD-1 mice. ISIS 598769, ISIS 598768, and ISIS 486178 were administered subcutaneously twice a week for 6 weeks at 50 mg/kg/wk. ISIS 445569 was administered subcutaneously twice a week for 6 weeks at 100 mg/kg/wk. After treatment, ALT, AST, and BUN levels were lower in ISIS 486178 and ISIS 598768 treated mice than in ISIS 445569 treated mice. After treatment, ALT and AST levels were lower in ISIS 598769 treated mice than in ISIS 445569 treated mice. Therefore, ISIS 598769, ISIS 598768, and ISIS 486178 are more tolerable than the comparator compound, ISIS 445569 in CD-1 mice.

[0646] In certain embodiments, the compounds disclosed herein are more tolerable than appropriate comparator compounds, such as ISIS 445569, when administered to Sprague-Dawley rats. In another example, as provided in Example 18 (described herein), ISIS 598769, ISIS 598768, and ISIS 486178 exhibited more favorable tolerability markers than ISIS 445569 when administered to Sprague-Dawley rats. ISIS 598769, ISIS 598768, and ISIS 486178 were administered subcutaneously twice a week for 6 weeks at 50 mg/kg/wk. ISIS 445569 was administered subcutaneously twice a week for 6 weeks at 100 mg/kg/wk. After treatment, ALT and AST levels were lower in ISIS 486178, ISIS 598769, and ISIS 598768 treated mice than in ISIS 445569 treated mice. Therefore ISIS 598769, ISIS 598768, and ISIS 486178 are more tolerable than the comparator compound, ISIS 445569 in Sprague-Dawley rats.

[0647] In certain embodiments, the compounds disclosed herein exhibit more favorable tolerability markers in cynomolgous monkeys than appropriate comparator compounds, such as ISIS 445569. In another example, as provided in Example 19 (described herein), ISIS 598769, ISIS 598768, and ISIS 486178 exhibited more favorable tolerability markers in cynomolgous monkeys including Alanine aminotransferase (ALT), aspartate aminotransferase (AST), lactate dehydrogenase (LDH), and creatine kinase (CK) assessment. In certain embodiments, ALT and AST levels are used as indicators of hepatotoxicity. For example, in certain embodiments, elevated ALT and AST levels indicate trauma to liver cells. In certain embodiments, elevated CK levels are associated with damage to cells in muscle tissue. In certain embodiments, elevated LDH levels are associated with cellular tissue damage.

[0648] In certain embodiments, the compounds disclosed herein are more tolerable than appropriate comparator compounds, such as ISIS 445569, when administered to cynomolgous monkeys. As provided in Example 19, groups of cynomolgous monkeys were treated with 40 mg/kg/wk of ISIS 598769, ISIS 598768, ISIS 486178, and ISIS 445569. Treatment with ISIS 445569 resulted in elevated ALT and AST levels at 93 days into treatment. Treatment with ISIS 598768, and ISIS 486178 resulted in lower ALT and AST levels at 58 and 93 days into treatment compared to ISIS 445569. Treatment with ISIS 598769, resulted in lower AST levels at 58 and 93 days into treatment and lower ALT levels at 93 days of treatment compared to ISIS 445569. Furthermore, the ALT and AST levels of monkeys receiving doses of ISIS 598769, ISIS 598768, and ISIS 486178 were consistent with the ALT and AST levels of monkeys given saline. Treatment with ISIS 445569 resulted in elevated LDH levels compared to the LDH levels measured in animals given ISIS 598769, ISIS 598768, and ISIS 486178 at 93 days into treatment. Additionally, treatment with ISIS 445569 resulted in elevated CK levels compared to the CK levels measured in animals given ISIS 598769, ISIS 598768, and ISIS 486178 at 93 days into treatment. Therefore, ISIS 598769, ISIS 598768, and ISIS 486178 are more tolerable than the comparator compound, ISIS 445569.

[0649] As the data discussed above demonstrate, ISIS 598769, ISIS 598768, and ISIS 486178 possess a wider range of well-tolerated doses at which ISIS 598769, ISIS 598768, and ISIS 486178 are active compared to the comparator compound, ISIS 445569. Additionally, the totality of the data presented in the examples herein and discussed above demonstrate that each of ISIS 598769, ISIS 598768, and ISIS 486178 possess a number of safety and activity advantages over the comparator compound, ISIS 445569. In other words, each of ISIS 598769, ISIS 598768, and ISIS 486178 are likely to be safer and more active drugs in humans than ISIS 445569.

[0650] In certain embodiments, ISIS 445569 is likely to be a safer and more active drug in humans for reducing CUGexp DMPK mRNA and\or treating conditions or symptoms associated with having myotonic dystrophy type 1 than the other compounds disclosed in WO 2012/012443 and/or WO 2012/012467.

[0651] In certain embodiments, ISIS 512497 has a better safety profile in primates and CD-1 mice than ISIS 445569. In certain embodiments, ISIS 512497 achieves greater knockdown of human DMPK nucleic acid in multiple muscle tissues when administered at the same dose and at lower doses than ISIS 445569.

[0652] In certain embodiments, ISIS 486178 has a better safety profile in mice, rats, and primates than ISIS 445569. In certain embodiments, ISIS 486178 achieves greater knockdown of human DMPK nucleic acid in one or more muscle tissues when administered at the same dose and at lower doses than ISIS 445569.

[0653] In certain embodiments, ISIS 570808 achieves much greater knockdown of human DMPK nucleic acid at least five different muscle tissues when administered at the same dose and at lower dose than ISIS 445569.

[0654] In certain embodiments, ISIS 594292 achieves greater knockdown of human DMPK nucleic acid in one or more muscle tissues when administered at the same dose as ISIS 445569. In certain embodiments, ISIS 486178 has a better safety profile in primates than ISIS 445569.

[0655] In certain embodiments, ISIS 569473 achieves greater knockdown of human DMPK nucleic acid in one or more muscle tissues when administered at the same dose as ISIS 445569. In certain embodiments, ISIS 569473 has a better safety profile in primates than ISIS 445569.

[0656] In certain embodiments, ISIS 594300 achieves greater knockdown of human DMPK nucleic acid in one or more muscle tissues when administered at the same dose as ISIS 445569. In certain embodiments, ISIS 594300 has a better safety profile in primates than ISIS 445569.

[0657] In certain embodiments, ISIS 598777 achieves greater knockdown of human DMPK nucleic acid in one or more muscle tissues when administered at the same dose as ISIS 445569. In certain embodiments, ISIS 598777 has a better safety profile in primates than ISIS 445569.

[0658] In certain embodiments, ISIS 598768 achieves greater knockdown of human DMPK nucleic acid in one or more muscle tissues when administered at the same dose as ISIS 445569. In certain embodiments, ISIS 598768 has a better safety profile in primates than ISIS 445569.

[0659] In certain embodiments, ISIS 598769 achieves greater knockdown of human DMPK nucleic acid in one or more muscle tissues when administered at the same dose as ISIS 445569. In certain embodiments, ISIS 598769 has a better safety profile in primates than ISIS 445569.

Nonlimiting Disclosure and Incorporation by Reference

[0660] While certain compounds, compositions and methods described herein have been described with specificity in accordance with certain embodiments, the following examples serve only to illustrate the compounds described herein and are not intended to limit the same. Each of the references, GenBank accession numbers, and the like recited in the present application is incorporated herein by reference in its entirety.

[0661] Although the sequence listing accompanying this filing identifies each sequence as either "RNA" or "DNA" as required, in reality, those sequences may be modified with any combination of chemical modifications. One of skill in the art will readily appreciate that such designation as "RNA" or "DNA" to describe modified oligonucleotides is, in certain instances, arbitrary. For example, an oligonucleotide comprising a nucleoside comprising a 2'-OH sugar moiety and a thymine base could be described as a DNA having a modified sugar (2'-OH for the natural 2'-H of DNA) or as an RNA having a modified base (thymine (methylated uracil) for natural uracil of RNA).

[0662] Accordingly, nucleic acid sequences provided herein, including, but not limited to those in the sequence listing, are intended to encompass nucleic acids containing any combination of natural or modified RNA and/or DNA, including, but not limited to such nucleic acids having modified nucleobases. By way of further example and without limitation, an oligomeric compound having the nucleobase sequence "ATCGATCG" encompasses any oligomeric compounds having such nucleobase sequence, whether modified or unmodified, including, but not limited to, such compounds comprising RNA bases, such as those having sequence "AUCGAUCG" and those having some DNA bases and some RNA bases such as "AUCGATCG" and oligomeric compounds having other modified or naturally occurring bases, such as "AT.sup.meeCGAUCG," wherein .sup.meC indicates a cytosine base comprising a methyl group at the 5-position.

EXAMPLES

Non-Limiting Disclosure and Incorporation by Reference

[0663] While certain compounds, compositions and methods described herein have been described with specificity in accordance with certain embodiments, the following examples serve only to illustrate the compounds described herein and are not intended to limit the same. Each of the references recited in the present application is incorporated herein by reference in its entirety.

Example 1

Design of Antisense Oligonucleotides Targeting Human Dystrophia Myotonica Protein Kinase (hDMPK)

[0664] A series of antisense oligonucleotides (ASOs) were designed to target hDMPK. The newly designed ASOs were prepared using standard oligonucleotide synthesis well known in the art and are described in Tables 1 and 2, below. Subscripts "s" indicate phosphorothioate internucleoside linkages; subscripts "k" indicate 6'-(S)--CH.sub.3 bicyclic nucleosides (cEt); subscripts "e" indicate 2'-O-methoxyethyl (MOE) modified nucleosides; and subscripts "d" indicate .beta.-D-2'-deoxyribonucleosides. ".sup.mC" indicates 5-methylcytosine nucleosides.

[0665] The antisense oligonucleotides are targeted to either SEQ ID NO: 1 (GENBANK Accession No. NM_001081560.1) and/or SEQ ID NO: 2 (the complement of GENBANK Accession No. NT_011109.15 truncated from nucleotides 18540696 to Ser. No. 18/555,106). "Start site" indicates the 5'-most nucleoside to which the gapmer is targeted in the human gene sequence. "Stop site" indicates the 3'-most nucleoside to which the gapmer is targeted human gene sequence.

TABLE-US-00001 TABLE 1 Design of antisense oligonucleotides targeting hDMPK and targeted to SEQ ID NO 2 Start Stop SEQ ID ISIS No. Composition (5' to 3') Motif Length Site Site No. 486178 A.sub.ks.sup.mC.sub.ksA.sub.ksA.sub.dsT.sub.dsA.sub.dsA.sub.dsA.sub- .ds kkk-10-kkk 16 13836 13851 23 T.sub.dsA.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsG.sub.dsA.sub.ksG.sub.ksG.sub- .k 445569 .sup.mC.sub.esG.sub.esG.sub.esA.sub.esG.sub.es.sup.mC.sub.dsG.sub.d- sG.sub.dsT.sub.dsT.sub.dsG.sub.ds e5-d10-e5 20 13226 13245 24 T.sub.dsG.sub.dsA.sub.dsA.sub.ds.sup.mC.sub.esT.sub.esG.sub.esG.sub.es.su- p.mC.sub.e 512497 G.sub.es.sup.mC.sub.esG.sub.es.sup.mC.sub.esA.sub.es.sup.mC.sub.ds.- sup.mC.sub.dsT.sub.dsT.sub.ds.sup.mC.sub.ds e5-d10-e5 20 8608 8627 25 .sup.mC.sub.ds.sup.mC.sub.dsG.sub.dsA.sub.dsA.sub.dsT.sub.esG.sub.esT.sub- .es.sup.mC.sub.es.sup.mC.sub.e 598768 .sup.mC.sub.es.sup.mC.sub.es.sup.mC.sub.ksG.sub.ksA.sub.dsA.sub.dsT- .sub.dsG.sub.ds eekk-d8-kkee 16 8603 8618 26 T.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsG.sub.dsA.sub.ks.sup.mC.sub.ksA.sub.e- sG.sub.e 594300 .sup.mC.sub.esG.sub.esG.sub.esA.sub.ksG.sub.ks.sup.mC.sub.dsG.sub.d- sG.sub.ds eeekk-d7-kkeee 17 13229 13245 27 T.sub.dsT.sub.dsG.sub.dsT.sub.dsG.sub.ksA.sub.ksA.sub.es.sup.mC.sub.esT.s- ub.e 594292 A.sub.es.sup.mC.sub.esA.sub.esA.sub.ksT.sub.ksA.sub.dsA.sub.dsA.sub- .dsT.sub.ds eeekk-d7-kkeee 17 13835 13851 28 A.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsG.sub.ksA.sub.ksG.sub.esG.sub.esA.sub- .e 569473 G.sub.ksA.sub.ks.sup.mC.sub.ksA.sub.dsA.sub.dsT.sub.ds.sup.mC.sub.d- sT.sub.ds kkk-d10-kkk 16 5082 5097 29 .sup.mC.sub.ds.sup.mC.sub.dsG.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsA.sub.ksG- .sub.ksG.sub.k 598769 T.sub.es.sup.mC.sub.es.sup.mC.sub.ks.sup.mC.sub.ksG.sub.dsA.sub.dsA- .sub.dsT.sub.ds eekk-d8-kkee 16 8604 8619 30 G.sub.dsT.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsG.sub.ksA.sub.ks.sup.mC.sub.e- sA.sub.e 570808 T.sub.ksG.sub.ksT.sub.ksA.sub.dsA.sub.dsT.sub.dsG.sub.dsT.sub.ds kkk-d10-kkk 16 10201 10216 31 T.sub.dsG.sub.dsT.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsA.sub.ksG.sub.ksT.sub- .k 598777 G.sub.esT.sub.esG.sub.ksT.sub.ksA.sub.dsA.sub.dsT.sub.dsG.sub.ds eekk-d8-kkee 16 10202 10217 32 T.sub.dsT.sub.dsG.sub.dsT.sub.ds.sup.mC.sub.ks.sup.mC.sub.ksA.sub.esG.sub- .e

TABLE-US-00002 TABLE 2 Design of antisense oligonucleotides targeting hDMPK and targeted to SEQ ID NO 1 Start Stop ISIS No. Composition (5' to 3' ) Motif Length Site Site 486178 A.sub.ks.sup.mC.sub.ksA.sub.ksA.sub.dsT.sub.dsA.sub.dsA.sub.dsA.sub- .ds kkk-10-kkk 16 2773 2788 T.sub.dsA.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsG.sub.dsA.sub.ksG.sub.ksG.sub- .k 445569 .sup.mC.sub.esG.sub.esG.sub.esA.sub.esG.sub.es.sup.mC.sub.dsG.sub.d- sG.sub.dsT.sub.dsT.sub.dsG.sub.ds e5-d10-e5 20 2163 2182 T.sub.dsG.sub.dsA.sub.dsA.sub.ds.sup.mC.sub.esT.sub.esG.sub.esG.sub.es.su- p.mC.sub.e 512497 G.sub.es.sup.mC.sub.esG.sub.es.sup.mC.sub.esA.sub.es.sup.mC.sub.ds.- sup.mC.sub.dsT.sub.dsT.sub.ds.sup.mC.sub.ds e5-d10-e5 20 1348 1367 .sup.mC.sub.ds.sup.mC.sub.dsG.sub.dsA.sub.dsA.sub.dsT.sub.esG.sub.esT.sub- .es.sup.mC.sub.es.sup.mC.sub.e 598768 .sup.mC.sub.es.sup.mC.sub.es.sup.mC.sub.ksG.sub.ksA.sub.dsA.sub.dsT- .sub.dsG.sub.ds eekk-d8-kkee 16 1343 1358 T.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsG.sub.dsA.sub.ks.sup.mC.sub.ksA.sub.e- sG.sub.e 594300 .sup.mC.sub.esG.sub.esG.sub.esA.sub.ksG.sub.ks.sup.mC.sub.dsG.sub.d- sG.sub.ds eeekk-d7-kkeee 17 2166 2182 T.sub.dsT.sub.dsG.sub.dsT.sub.dsG.sub.ksA.sub.ksA.sub.es.sup.mC.sub.esT.s- ub.e 594292 A.sub.es.sup.mC.sub.esA.sub.esA.sub.ksT.sub.ksA.sub.dsA.sub.dsA.sub- .dsT.sub.ds eeekk-d7-kkeee 17 2772 2788 A.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsG.sub.ksA.sub.ksG.sub.esG.sub.esA.sub- .e 569473 G.sub.ksA.sub.ks.sup.mC.sub.ksA.sub.dsA.sub.dsT.sub.ds.sup.mC.sub.d- sT.sub.ds kkk-d10-kkk 16 730 745 .sup.mC.sub.ds.sup.mC.sub.dsG.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsA.sub.ksG- .sub.ksG.sub.k 598769 T.sub.es.sup.mC.sub.es.sup.mC.sub.ks.sup.mC.sub.ksG.sub.dsA.sub.dsA- .sub.dsT.sub.ds eekk-d8-kkee 16 1344 1359 G.sub.dsT.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsG.sub.ksA.sub.ks.sup.mC.sub.e- sA.sub.e

Example 2

Antisense Inhibition of Human DMPK in Human Skeletal Muscle Cells (hSKMc)

[0666] Antisense oligonucleotides targeted to a human DMPK nucleic acid were tested for their effect on DMPK RNA transcript in vitro. Cultured hSKMc cells at a density of 20,000 cells per well were transfected using electroporation with 10,000 nM antisense oligonucleotide. After approximately 24 hours, RNA was isolated from the cells and DMPK transcript levels were measured by quantitative real-time PCR. DMPK RNA transcript levels were adjusted according to total RNA content, as measured by RIBOGREEN.RTM.. Results are presented as percent expression of DMPK, relative to untreated control cells.

[0667] The antisense oligonucleotides in Tables 3, 4, 5, and 6 are 5-10-5 gapmers, where the gap segment comprises ten 2'-deoxynucleosides and each wing segment comprises five 2'-MOE nucleosides. The internucleoside linkages throughout each gapmer are phosphorothioate (P.dbd.S) linkages. All cytosine residues throughout each gapmer are 5-methylcytosines. `Target start site` indicates the 5'-most nucleoside to which the antisense oligonucleotide is targeted in the human genomic gene sequence. `Target stop site` indicates the 3'-most nucleoside to which the antisense oligonucleotide is targeted in the human genomic sequence. All the antisense oligonucleotides listed in Table 3, 4, or 5 target SEQ ID NO: 1 (GENBANK Accession No. NM_001081560.1). All the antisense oligonucleotides listed in Table 6 target SEQ ID NO: 2 (the complement of GENBANK Accession No. NT_011109.15 truncated from nucleotides 18540696 to Ser. No. 18/555,106).

[0668] Several of the antisense oligonucleotides in Tables 2, 3, 4, and 5 demonstrated significant inhibition of DMPK mRNA levels under the conditions specified above.

TABLE-US-00003 TABLE 3 Inhibition of human DMPK RNA transcript in hSKMc by 5-10-5 gapmers targeting SEQ ID NO: 1 Start Site Stop Site SEQ ISIS % Target on Seq ID: on Seq ID No. Sequence Expression 1 ID: 1 NO. UTC N/A 100.0 N/A N/A 33 444401 TTGCACTTTGCGAACCAACG 7.3 2490 2509 34 512326 CGACACCTCGCCCCTCTTCA 13.4 528 547 35 512327 ACGACACCTCGCCCCTCTTC 40.8 529 548 36 512328 CACGACACCTCGCCCCTCTT 27.8 530 549 37 512329 GCACGACACCTCGCCCCTCT 16.5 531 550 38 512330 AGCACGACACCTCGCCCCTC 17.9 532 551 39 512331 AAGCACGACACCTCGCCCCT 18.8 533 552 40 512332 GAAGCACGACACCTCGCCCC 23.3 534 553 41 512333 GGAAGCACGACACCTCGCCC 28.1 535 554 42 512334 CGGAAGCACGACACCTCGCC 16.3 536 555 43 512335 ACGGAAGCACGACACCTCGC 28.7 537 556 44 512336 CACGGAAGCACGACACCTCG 15.9 538 557 45 512337 TCACGGAAGCACGACACCTC 18.8 539 558 46 512338 CTCACGGAAGCACGACACCT 16.4 540 559 47 512339 CCTCACGGAAGCACGACACC 20.2 541 560 48 512340 TCCTCACGGAAGCACGACAC 19.3 542 561 49 512341 CTCCTCACGGAAGCACGACA 15.2 543 562 50 512342 TCTCCTCACGGAAGCACGAC 16.2 544 563 51 512343 CTCTCCTCACGGAAGCACGA 16.4 545 564 52 512344 CCTCTCCTCACGGAAGCACG 15.7 546 565 53 512345 CCCTCTCCTCACGGAAGCAC 14.7 547 566 54 512346 TCCCTCTCCTCACGGAAGCA 20.6 548 567 55 512347 GTCCCTCTCCTCACGGAAGC 32.6 549 568 56 512348 CGTCCCTCTCCTCACGGAAG 31.5 550 569 57 512349 GGTCCCCATTCACCAACACG 41.6 568 587 58 512350 CGGTCCCCATTCACCAACAC 31.6 569 588 59 512351 CCGGTCCCCATTCACCAACA 38.1 570 589 60 512352 GCCGGTCCCCATTCACCAAC 55.5 571 590 61 512353 CGCCGGTCCCCATTCACCAA 42.9 572 591 62 512354 CCGCCGGTCCCCATTCACCA 35.7 573 592 63 512355 ACCGCCGGTCCCCATTCACC 51.4 574 593 64 512356 CACCGCCGGTCCCCATTCAC 34.4 575 594 65 512357 CCACCGCCGGTCCCCATTCA 40.4 576 595 66 512358 TCCACCGCCGGTCCCCATTC 35.5 577 596 67 512359 ATCCACCGCCGGTCCCCATT 41.7 578 597 68 512360 GATCCACCGCCGGTCCCCAT 51.0 579 598 69 512361 TGATCCACCGCCGGTCCCCA 35.9 580 599 70 512362 GTGATCCACCGCCGGTCCCC 53.2 581 600 71 512363 CGTGATCCACCGCCGGTCCC 28.2 582 601 72 512364 TTCTCATCCTGGAAGGCGAA 34.6 611 630 73 512365 GTTCTCATCCTGGAAGGCGA 57.1 612 631 74 512366 AGTTCTCATCCTGGAAGGCG 72.1 613 632 75 512367 GTAGTTCTCATCCTGGAAGG 47.1 615 634 76 512368 GGTAGTTCTCATCCTGGAAG 56.0 616 635 77 512369 AGGTAGTTCTCATCCTGGAA 48.3 617 636 78 512370 CAGGTAGTTCTCATCCTGGA 20.2 618 637 79 512371 TACAGGTAGTTCTCATCCTG 44.0 620 639 80 512372 GTACAGGTAGTTCTCATCCT 64.1 621 640 81 512373 GGTACAGGTAGTTCTCATCC 54.2 622 641 82 512374 AGGTACAGGTAGTTCTCATC 65.6 623 642 83 512375 CCAGGTACAGGTAGTTCTCA 45.7 625 644 84 512376 ACCAGGTACAGGTAGTTCTC 60.4 626 645 85 512377 GACCAGGTACAGGTAGTTCT 62.2 627 646 86 512378 TGACCAGGTACAGGTAGTTC 64.9 628 647 87 512379 CATGACCAGGTACAGGTAGT 39.2 630 649 88 512380 CCATGACCAGGTACAGGTAG 27.7 631 650 89 512381 TCCATGACCAGGTACAGGTA 21.6 632 651 90 512382 CTCCATGACCAGGTACAGGT 25.7 633 652 91 512383 ACTCCATGACCAGGTACAGG 28.6 634 653 92 512384 TACTCCATGACCAGGTACAG 23.7 635 654 93 512385 ATACTCCATGACCAGGTACA 20.8 636 655 94 512386 AATACTCCATGACCAGGTAC 22.0 637 656 95 512387 TAATACTCCATGACCAGGTA 14.7 638 657 96 512388 CGTAATACTCCATGACCAGG 10.4 640 659 97 512389 AGCAGTGTCAGCAGGTCCCC 15.0 665 684 98 512390 CAGCAGTGTCAGCAGGTCCC 13.0 666 685 99 512391 TCAGCAGTGTCAGCAGGTCC 22.3 667 686 100 512392 CTCAGCAGTGTCAGCAGGTC 16.4 668 687 101 512393 GCTCAGCAGTGTCAGCAGGT 22.2 669 688 102 512394 TGCTCAGCAGTGTCAGCAGG 26.2 670 689 103 512395 TTGCTCAGCAGTGTCAGCAG 27.4 671 690 104 512396 CTTGCTCAGCAGTGTCAGCA 15.7 672 691 105 512397 ACTTGCTCAGCAGTGTCAGC 43.5 673 692 106 512398 AACTTGCTCAGCAGTGTCAG 26.9 674 693 107 512399 AAACTTGCTCAGCAGTGTCA 20.0 675 694 108 512400 CAAACTTGCTCAGCAGTGTC 23.1 676 695 109 512401 CCAAACTTGCTCAGCAGTGT 20.5 677 696 110 512402 CCCAAACTTGCTCAGCAGTG 13.5 678 697 33

TABLE-US-00004 TABLE 4 Inhibition of human DMPK RNA transcript in hSKMc by 5-10-5 gapmers targeting SEQ ID NO: 1 Start Site Stop Site SEQ ISIS % Target on Seq ID: on Seq ID No. Sequence Expression 1 ID: 1 NO. UTC N/A 100 N/A N/A 444401 TTGCACTTTGCGAACCAACG 13.4 2490 2509 33 512480 GTGAGCCCGTCCTCCACCAA 29.8 1310 1329 111 512481 AGTGAGCCCGTCCTCCACCA 15.6 1311 1330 112 512482 CAGTGAGCCCGTCCTCCACC 10.7 1312 1331 113 512483 GCAGTGAGCCCGTCCTCCAC 33.3 1313 1332 114 512484 GGCAGTGAGCCCGTCCTCCA 9.6 1314 1333 115 512485 TGGCAGTGAGCCCGTCCTCC 8.8 1315 1334 116 512486 CATGGCAGTGAGCCCGTCCT 10.5 1317 1336 117 512487 CCATGGCAGTGAGCCCGTCC 10.1 1318 1337 118 512488 TCCATGGCAGTGAGCCCGTC 13.7 1319 1338 119 512489 CTCCATGGCAGTGAGCCCGT 16.9 1320 1339 120 512490 TCTCCATGGCAGTGAGCCCG 29.1 1321 1340 121 512491 GTCTCCATGGCAGTGAGCCC 41.3 1322 1341 122 512492 CCTTCCCGAATGTCCGACAG 8.8 1343 1362 123 512493 ACCTTCCCGAATGTCCGACA 12.1 1344 1363 124 512494 CACCTTCCCGAATGTCCGAC 6 1345 1364 125 512495 GCACCTTCCCGAATGTCCGA 8.5 1346 1365 126 512496 CGCACCTTCCCGAATGTCCG 5.6 1347 1366 127 512497 GCGCACCTTCCCGAATGTCC 7.7 1348 1367 25 512498 GGCGCACCTTCCCGAATGTC 15 1349 1368 128 512499 ACAAAAGGCAGGTGGACCCC 22.8 1373 1392 129 512500 CACAAAAGGCAGGTGGACCC 22 1374 1393 130 512501 CCACAAAAGGCAGGTGGACC 16.4 1375 1394 131 512502 CCCACAAAAGGCAGGTGGAC 15.8 1376 1395 132 512503 GCCCACAAAAGGCAGGTGGA 25.1 1377 1396 133 512504 AGCCCACAAAAGGCAGGTGG 24.7 1378 1397 134 512505 TAGCCCACAAAAGGCAGGTG 20.7 1379 1398 135 512506 GTAGCCCACAAAAGGCAGGT 20.7 1380 1399 136 512507 AGTAGCCCACAAAAGGCAGG 27.8 1381 1400 137 512508 GAGTAGCCCACAAAAGGCAG 43.9 1382 1401 138 512509 GGAGTAGCCCACAAAAGGCA 29.9 1383 1402 139 512510 AGGAGTAGCCCACAAAAGGC 31.9 1384 1403 140 512511 TAGGAGTAGCCCACAAAAGG 59.9 1385 1404 141 512512 GTAGGAGTAGCCCACAAAAG 40.1 1386 1405 142 512513 AGTAGGAGTAGCCCACAAAA 48.1 1387 1406 143 512514 GAGTAGGAGTAGCCCACAAA 53.3 1388 1407 144 512515 GGAGTAGGAGTAGCCCACAA 24.7 1389 1408 145 512516 AGGAGTAGGAGTAGCCCACA 22.1 1390 1409 146 512517 CAGGAGTAGGAGTAGCCCAC 15.4 1391 1410 147 512518 GCAGGAGTAGGAGTAGCCCA 32.8 1392 1411 148 512519 TGCAGGAGTAGGAGTAGCCC 37.6 1393 1412 149 512520 ATGCAGGAGTAGGAGTAGCC 47.4 1394 1413 150 512521 CATGCAGGAGTAGGAGTAGC 67.2 1395 1414 151 512522 CCATGCAGGAGTAGGAGTAG 58.8 1396 1415 152 512523 GCCATGCAGGAGTAGGAGTA 42.4 1397 1416 153 512524 GGCCATGCAGGAGTAGGAGT 34.1 1398 1417 154 512525 GGGCCATGCAGGAGTAGGAG 44.5 1399 1418 155 512526 AGGGCCATGCAGGAGTAGGA 42 1400 1419 156 512527 GAGGGCCATGCAGGAGTAGG 46.3 1401 1420 157 512528 CTGAGGGCCATGCAGGAGTA 25.3 1403 1422 158 512529 CCTGAGGGCCATGCAGGAGT 28.1 1404 1423 159 512530 CCCTGAGGGCCATGCAGGAG 22.8 1405 1424 160 512531 TCCCTGAGGGCCATGCAGGA 25.7 1406 1425 161 512532 GTCCCTGAGGGCCATGCAGG 17 1407 1426 162 512533 TGTCCCTGAGGGCCATGCAG 18.9 1408 1427 163 512534 CTGTCCCTGAGGGCCATGCA 27.3 1409 1428 164 512535 ACTGTCCCTGAGGGCCATGC 16.5 1410 1429 165 512536 CACTGTCCCTGAGGGCCATG 26 1411 1430 166 512537 TCACTGTCCCTGAGGGCCAT 22.7 1412 1431 167 512538 CTCACTGTCCCTGAGGGCCA 20.2 1413 1432 168 512539 CCTCACTGTCCCTGAGGGCC 19.3 1414 1433 169 512540 ACCTCACTGTCCCTGAGGGC 31 1415 1434 170 512541 GACCTCACTGTCCCTGAGGG 51.4 1416 1435 171 512542 GGACCTCACTGTCCCTGAGG 28 1417 1436 172 512543 GGGACCTCACTGTCCCTGAG 42.6 1418 1437 173 512544 CCTCCAGTTCCATGGGTGTG 16.7 1444 1463 174 512545 GCCTCCAGTTCCATGGGTGT 21.9 1445 1464 175 512546 GGCCTCCAGTTCCATGGGTG 19 1446 1465 176 512547 CGGCCTCCAGTTCCATGGGT 14.9 1447 1466 177 512548 TCGGCCTCCAGTTCCATGGG 23 1448 1467 178 512549 CTCGGCCTCCAGTTCCATGG 15.7 1449 1468 179 512550 GCTCGGCCTCCAGTTCCATG 16.2 1450 1469 180 512551 TGCTCGGCCTCCAGTTCCAT 17.7 1451 1470 181 512552 CTGCTCGGCCTCCAGTTCCA 18.4 1452 1471 182 512553 GCTGCTCGGCCTCCAGTTCC 22 1453 1472 183 512554 AGCTGCTCGGCCTCCAGTTC 32.4 1454 1473 184 512555 CAGCTGCTCGGCCTCCAGTT 15.7 1455 1474 185 512556 GCAGCTGCTCGGCCTCCAGT 16.3 1456 1475 186

TABLE-US-00005 TABLE 5 Inhibition of human DMPK RNA transcript in hSKMc by 5-10-5 gapmers targeting SEQ ID NO: 1 Start Site Stop Site SEQ ISIS % Target on Seq ID: on Seq ID No. Sequence Expression 1 ID: 1 NO. UTC N/A 100.0 N/A N/A 444401 TTGCACTTTGCGAACCAACG 7.0 2490 2509 33 512557 AGCAGCTGCTCGGCCTCCAG 25.2 1457 1476 187 512558 AAGCAGCTGCTCGGCCTCCA 16.1 1458 1477 188 512559 CAAGCAGCTGCTCGGCCTCC 21.9 1459 1478 189 512560 TCAAGCAGCTGCTCGGCCTC 24.8 1460 1479 190 512561 CTCAAGCAGCTGCTCGGCCT 19.8 1461 1480 191 512562 GCTCAAGCAGCTGCTCGGCC 11.6 1462 1481 192 512563 GGCTCAAGCAGCTGCTCGGC 19.8 1463 1482 193 512564 TGGCTCAAGCAGCTGCTCGG 31.9 1464 1483 194 512565 GTGGCTCAAGCAGCTGCTCG 27.5 1465 1484 195 512566 TGTGGCTCAAGCAGCTGCTC 35.4 1466 1485 196 512567 GTGTGGCTCAAGCAGCTGCT 24.8 1467 1486 197 512568 CCACTTCAGCTGTTTCATCC 43.1 1525 1544 198 512569 TGCCACTTCAGCTGTTTCAT 35.0 1527 1546 199 512570 CTGCCACTTCAGCTGTTTCA 27.8 1528 1547 200 512571 ACTGCCACTTCAGCTGTTTC 78.9 1529 1548 201 512572 AACTGCCACTTCAGCTGTTT 36.4 1530 1549 202 512573 GAACTGCCACTTCAGCTGTT 30.3 1531 1550 203 512574 GGAACTGCCACTTCAGCTGT 66.7 1532 1551 204 512575 TGGAACTGCCACTTCAGCTG 22.6 1533 1552 205 512576 CTGGAACTGCCACTTCAGCT 22.9 1534 1553 206 512577 GCTGGAACTGCCACTTCAGC 59.5 1535 1554 207 512578 CGCTGGAACTGCCACTTCAG 24.9 1536 1555 208 512579 CCGCTGGAACTGCCACTTCA 42.5 1537 1556 209 512580 GCCGCTGGAACTGCCACTTC 20.0 1538 1557 210 512581 AGCCGCTGGAACTGCCACTT 19.4 1539 1558 211 512582 CTCAGCCTCTGCCGCAGGGA 22.1 1560 1579 212 512583 CCTCAGCCTCTGCCGCAGGG 33.7 1561 1580 213 512584 GGCCTCAGCCTCTGCCGCAG 24.6 1563 1582 214 512585 CGGCCTCAGCCTCTGCCGCA 55.1 1564 1583 215 512586 TCGGCCTCAGCCTCTGCCGC 60.8 1565 1584 216 512587 CTCGGCCTCAGCCTCTGCCG 31.8 1566 1585 217 512588 CCTCGGCCTCAGCCTCTGCC 16.4 1567 1586 218 512589 ACCTCGGCCTCAGCCTCTGC 31.1 1568 1587 219 512590 CACCTCGGCCTCAGCCTCTG 39.7 1569 1588 220 512591 TCACCTCGGCCTCAGCCTCT 24.8 1570 1589 221 512592 GTCACCTCGGCCTCAGCCTC 28.7 1571 1590 222 512593 CGTCACCTCGGCCTCAGCCT 20.3 1572 1591 223 512594 AGCACCTCCTCCTCCAGGGC 18.4 1610 1629 224 512595 GAGCACCTCCTCCTCCAGGG 19.9 1611 1630 225 512596 TGAGCACCTCCTCCTCCAGG 15.6 1612 1631 226 512597 GTGAGCACCTCCTCCTCCAG 22.3 1613 1632 227 512598 GGTGAGCACCTCCTCCTCCA 19.4 1614 1633 228 512599 GGGTGAGCACCTCCTCCTCC 17.3 1615 1634 229 512600 CGGGTGAGCACCTCCTCCTC 12.2 1616 1635 230 512601 CCGGGTGAGCACCTCCTCCT 15.9 1617 1636 231 512602 GCCGGGTGAGCACCTCCTCC 15.7 1618 1637 232 512603 TGCCGGGTGAGCACCTCCTC 15.1 1619 1638 233 512604 CTGCCGGGTGAGCACCTCCT 24.5 1620 1639 234 512605 TCTGCCGGGTGAGCACCTCC 33.8 1621 1640 235 512606 GCTCTGCCGGGTGAGCACCT 26.1 1623 1642 236 512607 GGCTCTGCCGGGTGAGCACC 50.4 1624 1643 237 512608 AGGCTCTGCCGGGTGAGCAC 42.9 1625 1644 238 512609 CAGGCTCTGCCGGGTGAGCA 39.2 1626 1645 239 512610 TCAGGCTCTGCCGGGTGAGC 20.2 1627 1646 240 512611 GCTCAGGCTCTGCCGGGTGA 22.5 1629 1648 241 512612 CGGCTCAGGCTCTGCCGGGT 27.0 1631 1650 242 512613 CCGGCTCAGGCTCTGCCGGG 68.8 1632 1651 243 512614 CCCGGCTCAGGCTCTGCCGG 58.8 1633 1652 244 512615 TCCCGGCTCAGGCTCTGCCG 24.8 1634 1653 245 512616 CTCCCGGCTCAGGCTCTGCC 10.4 1635 1654 246 512617 TCTCCCGGCTCAGGCTCTGC 12.8 1636 1655 247 512618 ATCTCCCGGCTCAGGCTCTG 13.3 1637 1656 248 512619 CATCTCCCGGCTCAGGCTCT 7.7 1638 1657 249 512620 CCATCTCCCGGCTCAGGCTC 2.8 1639 1658 250 512621 TCCATCTCCCGGCTCAGGCT 2.6 1640 1659 251 512622 CTCCATCTCCCGGCTCAGGC 1.5 1641 1660 252 512623 CCTCCATCTCCCGGCTCAGG 1.4 1642 1661 253 512624 GCCTCCATCTCCCGGCTCAG 2.0 1643 1662 254 512625 GGCCTCCATCTCCCGGCTCA 8.3 1644 1663 255 512626 TGGCCTCCATCTCCCGGCTC 9.4 1645 1664 256 512627 ATGGCCTCCATCTCCCGGCT 6.3 1646 1665 257 512628 GATGGCCTCCATCTCCCGGC 2.7 1647 1666 258 512629 GGATGGCCTCCATCTCCCGG 1.3 1648 1667 259 512630 CGGATGGCCTCCATCTCCCG 1.5 1649 1668 260 512631 GCGGATGGCCTCCATCTCCC 2.4 1650 1669 261 512632 TGCGGATGGCCTCCATCTCC 2.2 1651 1670 262 512633 GTTCCGAGCCTCTGCCTCGC 29.2 1701 1720 263

TABLE-US-00006 TABLE 6 Inhibition of human DMPK RNA transcript in hSKMc by 5-10-5 gapmers targeting SEQ ID NO: 2 Start Site Stop Site SEQ ISIS % Target on Seq ID: on Seq ID No. Sequence Expression 2 ID: 2 NO. UTC N/A 100.0 N/A N/A 444401 TTGCACTTTGCGAACCAACG 7.0 13553 13572 33 444436 GTCGGAGGACGAGGTCAATA 9.7 13748 13767 264 512634 AGGGCCTCAGCCTGGCCGAA 31.7 13452 13471 265 512635 CAGGGCCTCAGCCTGGCCGA 39.5 13453 13472 266 512636 GTCAGGGCCTCAGCCTGGCC 20.5 13455 13474 267 512637 CGTCAGGGCCTCAGCCTGGC 23.3 13456 13475 268 512638 AGCAAATTTCCCGAGTAAGC 14.7 13628 13647 269 512639 AAGCAAATTTCCCGAGTAAG 21.2 13629 13648 270 512640 AAAAGCAAATTTCCCGAGTA 23.0 13631 13650 271 512641 CAAAAGCAAATTTCCCGAGT 19.7 13632 13651 272 512642 GCAAAAGCAAATTTCCCGAG 26.6 13633 13652 273 512643 GGCAAAAGCAAATTTCCCGA 12.8 13634 13653 274 512644 TGGCAAAAGCAAATTTCCCG 12.2 13635 13654 275 512645 TTTGGCAAAAGCAAATTTCC 24.2 13637 13656 276 512646 GTTTGGCAAAAGCAAATTTC 25.5 13638 13657 277 512647 GGGTTTGGCAAAAGCAAATT 43.0 13640 13659 278 512648 CGGGTTTGGCAAAAGCAAAT 27.2 13641 13660 279 512649 AAGCGGGTTTGGCAAAAGCA 27.0 13644 13663 280 512650 AATATCCAAACCGCCGAAGC 45.7 13728 13747 281 512651 AAATATCCAAACCGCCGAAG 56.6 13729 13748 282 512652 ATAAATATCCAAACCGCCGA 39.0 13731 13750 283 512653 AATAAATATCCAAACCGCCG 34.7 13732 13751 284 512654 TCAATAAATATCCAAACCGC 34.7 13734 13753 285 512655 GTCAATAAATATCCAAACCG 19.1 13735 13754 286 512656 GGTCAATAAATATCCAAACC 24.3 13736 13755 287 512657 AGGTCAATAAATATCCAAAC 23.5 13737 13756 288 512658 GAGGTCAATAAATATCCAAA 24.2 13738 13757 289 512659 ACGAGGTCAATAAATATCCA 28.3 13740 13759 290 512660 GACGAGGTCAATAAATATCC 17.8 13741 13760 291 512661 AGGACGAGGTCAATAAATAT 45.7 13743 13762 292 512662 GAGGACGAGGTCAATAAATA 27.6 13744 13763 293 512663 CGGAGGACGAGGTCAATAAA 15.8 13746 13765 294 512664 TCGGAGGACGAGGTCAATAA 10.8 13747 13766 295 512665 AGTCGGAGGACGAGGTCAAT 15.4 13749 13768 296 512666 GAGTCGGAGGACGAGGTCAA 18.8 13750 13769 297 512667 GCGAGTCGGAGGACGAGGTC 26.0 13752 13771 298 512668 AGCGAGTCGGAGGACGAGGT 21.7 13753 13772 299 512669 CAGCGAGTCGGAGGACGAGG 13.7 13754 13773 300 512670 TCAGCGAGTCGGAGGACGAG 16.5 13755 13774 301 512671 GTCAGCGAGTCGGAGGACGA 17.4 13756 13775 302 512672 CTGTCAGCGAGTCGGAGGAC 25.2 13758 13777 303 512673 CCTGTCAGCGAGTCGGAGGA 18.4 13759 13778 304 512674 AGCCTGTCAGCGAGTCGGAG 16.8 13761 13780 305 512675 GTCTCAGTGCATCCAAAACG 11.8 13807 13826 306 512676 GGTCTCAGTGCATCCAAAAC 17.7 13808 13827 307 512677 GGGTCTCAGTGCATCCAAAA 11.2 13809 13828 308 512678 GGAGGGCCTTTTATTCGCGA 17.8 13884 13903 309 512679 TGGAGGGCCTTTTATTCGCG 13.2 13885 13904 310 512680 ATGGAGGGCCTTTTATTCGC 19.3 13886 13905 311 512681 GATGGAGGGCCTTTTATTCG 30.5 13887 13906 312 512682 AGATGGAGGGCCTTTTATTC 50.8 13888 13907 313 512683 CAGATGGAGGGCCTTTTATT 46.1 13889 13908 314 512684 GCAGATGGAGGGCCTTTTAT 50.4 13890 13909 315 512685 CCCTCAGGCTCTCTGCTTTA 34.7 655 674 316 512686 GCCCTCAGGCTCTCTGCTTT 47.9 656 675 317 512687 AGCCCTCAGGCTCTCTGCTT 47.4 657 676 318 512688 TAGCCCTCAGGCTCTCTGCT 54.1 658 677 319 512689 TTAGCCCTCAGGCTCTCTGC 48.0 659 678 320 512690 TTTAGCCCTCAGGCTCTCTG 50.7 660 679 321 512691 ATTTAGCCCTCAGGCTCTCT 47.3 661 680 322 512692 AATTTAGCCCTCAGGCTCTC 44.8 662 681 323 512693 AAATTTAGCCCTCAGGCTCT 39.2 663 682 324 512694 TAAATTTAGCCCTCAGGCTC 48.0 664 683 325 512695 TTAAATTTAGCCCTCAGGCT 54.9 665 684 326 512696 GTTAAATTTAGCCCTCAGGC 48.1 666 685 327 512697 AGTTAAATTTAGCCCTCAGG 39.3 667 686 328 512698 CAGTTAAATTTAGCCCTCAG 47.5 668 687 329 512699 ACAGTTAAATTTAGCCCTCA 68.2 669 688 330 512700 GACAGTTAAATTTAGCCCTC 59.2 670 689 331 512701 GGACAGTTAAATTTAGCCCT 63.7 671 690 332 512702 CGGACAGTTAAATTTAGCCC 50.7 672 691 333 512703 TCGGACAGTTAAATTTAGCC 39.6 673 692 334 512704 CTCGGACAGTTAAATTTAGC 36.5 674 693 335 512705 ACTCGGACAGTTAAATTTAG 59.1 675 694 336 512706 GACTCGGACAGTTAAATTTA 50.0 676 695 337 512707 CGACTCGGACAGTTAAATTT 63.0 677 696 338 512708 CCGACTCGGACAGTTAAATT 34.3 678 697 339 512709 TCCGACTCGGACAGTTAAAT 39.5 679 698 340

Example 3

Design of Antisense Oligonucleotides Targeting Human Dystrophia Myotonica Protein Kinase (hDMPK)

[0669] A series of antisense oligonucleotides (ASOs) were designed to target hDMPK. The newly designed ASOs were prepared using standard oligonucleotide synthesis well known in the art and are described in Table 7, below. Subscripts "s" indicate phosphorothioate internucleoside linkages; subscripts "k" indicate 6'-(S)--CH.sub.3 bicyclic nucleosides (cEt); subscripts "e" indicate 2'-O-methoxyethyl (MOE) modified nucleosides; and subscripts "d" indicate .beta.-D-2'-deoxyribonucleosides. ".sup.mC" indicates 5-methylcytosine nucleosides.

[0670] The antisense oligonucleotides targeted to a human DMPK nucleic acid were tested for their effect on DMPK RNA transcript in vitro. Cultured HepG2 cells at a density of 20,000 cells per well were transfected using electroporation with 4,500 nM antisense oligonucleotide. After approximately 24 hours, RNA was isolated from the cells and DMPK transcript levels were measured by quantitative real-time PCR. DMPK RNA transcript levels were adjusted according to total RNA content, as measured by RIBOGREEN.RTM.. Results are presented as percent expression of DMPK, relative to untreated control cells. `Target start site` indicates the 5'-most nucleoside to which the antisense oligonucleotide is targeted in the human genomic gene sequence. `Target stop site` indicates the 3'-most nucleoside to which the antisense oligonucleotide is targeted in the human genomic sequence. All the antisense oligonucleotides listed in Table 7 target SEQ ID NO: 1 (GENBANK Accession No. NM_001081560.1).

[0671] Several of the antisense oligonucleotides demonstrated significant inhibition of DMPK mRNA levels under the conditions specified above.

TABLE-US-00007 TABLE 7 Inhibition of human DMPK RNA transcript in HepG2 cells targeting SEQ ID NO: 1 Start Site Stop Site Seq ISIS % Target on Seq ID: on Seq ID No. Sequence Expression 1 ID: 1 No. UTC N/A 100 N/A N/A 533424 T.sub.es.sup.mC.sub.esT.sub.es.sup.mC.sub.ds.sup.mC.sub.dsT.sub.ds.- sup.mC.sub.dsA.sub.ds.sup.mC.sub.dsG.sub.dsG.sub.dsA.sub.dsA.sub.dsG.sub.k- s.sup.mC.sub.ksA.sub.k 34.4 548 563 341 533425 .sup.mC.sub.esT.sub.es.sup.mC.sub.esT.sub.ds.sup.mC.sub.ds.sup.mC.s- ub.dsT.sub.ds.sup.mC.sub.dsA.sub.ds.sup.mC.sub.dsG.sub.dsG.sub.dsA.sub.dsA- .sub.ksG.sub.ks.sup.mC.sub.k 32.1 549 564 342 533426 .sup.mC.sub.esienC.sub.esT.sub.es.sup.mC.sub.dsT.sub.ds.sup.mC.sub.- ds.sup.mC.sub.dsT.sub.ds.sup.mC.sub.dsA.sub.ds.sup.mC.sub.dsG.sub.dsG.sub.- dsA.sub.ksA.sub.ksG.sub.k 52.1 550 565 343 533427 A.sub.esA.sub.esA.sub.es.sup.mC.sub.dsT.sub.dsT.sub.dsG.sub.ds.sup.- mC.sub.dsT.sub.ds.sup.mC.sub.dsA.sub.dsG.sub.ds.sup.mC.sub.dsA.sub.ksG.sub- .ksT.sub.k 36.8 679 694 344 533428 .sup.mC.sub.esA.sub.esA.sub.esA.sub.ds.sup.mC.sub.dsT.sub.dsT.sub.d- sG.sub.ds.sup.mC.sub.dsT.sub.ds.sup.mC.sub.dsA.sub.dsG.sub.ds.sup.mC.sub.k- sA.sub.ksG.sub.k 59.9 680 695 345 533429 .sup.mC.sub.es.sup.mC.sub.esA.sub.esA.sub.dsA.sub.ds.sup.mC.sub.dsT- .sub.dsT.sub.dsG.sub.ds.sup.mC.sub.dsT.sub.ds.sup.mC.sub.dsA.sub.dsG.sub.k- s.sup.mC.sub.ksA.sub.k 39.3 681 696 346 533430 .sup.mC.sub.es.sup.mC.sub.es.sup.mC.sub.esA.sub.dsA.sub.dsA.sub.ds.- sup.mC.sub.dsT.sub.dsT.sub.dsG.sub.ds.sup.mC.sub.dsT.sub.ds.sup.mC.sub.dsA- .sub.ksG.sub.ks.sup.mC.sub.k 37.6 682 697 347 533431 .sup.mC.sub.es.sup.mC.sub.es.sup.mC.sub.es.sup.mC.sub.dsA.sub.dsA.s- ub.dsA.sub.ds.sup.mC.sub.dsT.sub.dsT.sub.dsG.sub.ds.sup.mC.sub.dsT.sub.ds.- sup.mC.sub.ksA.sub.ksG.sub.k 39.6 683 698 348 533432 T.sub.es.sup.mC.sub.es.sup.mC.sub.es.sup.mC.sub.ds.sup.mC.sub.dsA.s- ub.dsA.sub.dsA.sub.ds.sup.mC.sub.dsT.sub.dsT.sub.dsG.sub.ds.sup.mC.sub.dsT- .sub.ks.sup.mC.sub.ksA.sub.k 52.1 684 699 349 533433 G.sub.esT.sub.esT.sub.esT.sub.dsG.sub.dsA.sub.dsT.sub.dsG.sub.dsT.s- ub.ds.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsT.sub.dsG.sub.ksT.sub.ksG.s- ub.k 53.9 782 797 350 533434 G.sub.esG.sub.esT.sub.esT.sub.dsT.sub.dsG.sub.dsA.sub.dsT.sub.dsG.s- ub.dsT.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsT.sub.ksG.sub.ksT.s- ub.k 38.1 783 798 351 533435 G.sub.esG.sub.esG.sub.esT.sub.dsT.sub.dsT.sub.dsG.sub.dsA.sub.dsT.s- ub.dsG.sub.dsT.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.ksT.sub.ksG.s- ub.k 43.7 784 799 352 533436 A.sub.es.sup.mC.sub.esA.sub.esG.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsT- .sub.dsG.sub.ds.sup.mC.sub.dsA.sub.dsG.sub.dsG.sub.dsA.sub.dsT.sub.ks.sup.- mC.sub.ksT.sub.k 29.5 927 942 353 533437 .sup.mC.sub.esA.sub.es.sup.mC.sub.esA.sub.dsG.sub.ds.sup.mC.sub.ds.- sup.mC.sub.dsT.sub.dsG.sub.ds.sup.mC.sub.dsA.sub.dsG.sub.dsG.sub.dsA.sub.k- sT.sub.ks.sup.mC.sub.k 48.6 928 943 354 533438 .sup.mC.sub.es.sup.mC.sub.esA.sub.es.sup.mC.sub.dsA.sub.dsG.sub.ds.- sup.mC.sub.ds.sup.mC.sub.dsT.sub.dsG.sub.ds.sup.mC.sub.dsA.sub.dsG.sub.dsG- .sub.ksA.sub.ksT.sub.k 46.9 929 944 355 533439 .sup.mC.sub.es.sup.mC.sub.es.sup.mC.sub.esA.sub.ds.sup.mC.sub.dsA.s- ub.dsG.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsT.sub.dsG.sub.ds.sup.mC.sub.dsA.s- ub.dsG.sub.ksG.sub.ksA.sub.k 43.6 930 945 356 533440 G.sub.es.sup.mC.sub.es.sup.mC.sub.es.sup.mC.sub.dsA.sub.ds.sup.mC.s- ub.dsA.sub.dsG.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsT.sub.dsG.sub.ds.sup.mC.s- ub.dsA.sub.ksG.sub.ksG.sub.k 26.9 931 946 357 533441 .sup.mC.sub.esG.sub.es.sup.mC.sub.es.sup.mC.sub.ds.sup.mC.sub.dsA.s- ub.ds.sup.mC.sub.dsA.sub.dsG.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsT.sub.dsG.s- ub.ds.sup.mC.sub.ksA.sub.ksG.sub.k 31.3 932 947 358 533442 .sup.mC.sub.es.sup.mC.sub.esG.sub.es.sup.mC.sub.ds.sup.mC.sub.ds.su- p.mC.sub.dsA.sub.ds.sup.mC.sub.dsA.sub.dsG.sub.ds.sup.mC.sub.ds.sup.mC.sub- .dsT.sub.dsG.sub.ks.sup.mC.sub.ksA.sub.k 20.5 933 948 359 533443 A.sub.es.sup.mC.sub.es.sup.mC.sub.esG.sub.ds.sup.mC.sub.ds.sup.mC.s- ub.ds.sup.mC.sub.dsA.sub.ds.sup.mC.sub.dsA.sub.dsG.sub.ds.sup.mC.sub.ds.su- p.mC.sub.dsT.sub.ksG.sub.ks.sup.mC.sub.k 13.7 934 949 360 533444 .sup.mC.sub.esA.sub.es.sup.mC.sub.es.sup.mC.sub.dsG.sub.ds.sup.mC.s- ub.ds.sup.mC.sub.ds.sup.mC.sub.dsA.sub.ds.sup.mC.sub.dsA.sub.dsG.sub.ds.su- p.mC.sub.ds.sup.mC.sub.ksT.sub.ksG.sub.k 29.4 935 950 361 533445 .sup.mC.sub.es.sup.mC.sub.esA.sub.es.sup.mC.sub.ds.sup.mC.sub.dsG.s- ub.ds.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsA.sub.ds.sup.mC.sub.dsA.sub- .dsG.sub.ds.sup.mC.sub.ks.sup.mC.sub.ksT.sub.k 32 936 951 362 533446 .sup.mC.sub.es.sup.mC.sub.es.sup.mC.sub.esA.sub.ds.sup.mC.sub.ds.su- p.mC.sub.dsG.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsA.sub.ds.sup.- mC.sub.dsA.sub.dsG.sub.ks.sup.mC.sub.ks.sup.mC.sub.k 8.3 937 952 363 533447 G.sub.es.sup.mC.sub.es.sup.mC.sub.es.sup.mC.sub.dsA.sub.ds.sup.mC.s- ub.ds.sup.mC.sub.dsG.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsA.sub- .ds.sup.mC.sub.dsA.sub.ksG.sub.ks.sup.mC.sub.k 18.3 938 953 364 533448 .sup.mC.sub.es.sup.mC.sub.esA.sub.esG.sub.dsG.sub.ds.sup.mC.sub.ds.- sup.mC.sub.ds.sup.mC.sub.dsA.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsG.sub.ds.su- p.mC.sub.ds.sup.mC.sub.ks.sup.mC.sub.ksA.sub.k 19.4 942 957 365 533449 .sup.mC.sub.es.sup.mC.sub.es.sup.mC.sub.esA.sub.dsG.sub.dsG.sub.ds.- sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsA.sub.ds.sup.mC.sub.ds.sup.mC.sub- .dsG.sub.ds.sup.mC.sub.ks.sup.mC.sub.ks.sup.mC.sub.k 24.2 943 958 366 533450 T.sub.es.sup.mC.sub.es.sup.mC.sub.es.sup.mC.sub.dsA.sub.dsG.sub.dsG- .sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsA.sub.ds.sup.mC.sub.ds.su- p.mC.sub.dsG.sub.ks.sup.mC.sub.ks.sup.mC.sub.k 39.2 944 959 367 533451 T.sub.esG.sub.es.sup.mC.sub.es.sup.mC.sub.dsT.sub.dsG.sub.dsT.sub.d- s.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsA.sub.dsG.sub.dsG.sub.ds.sup.mC- .sub.ks.sup.mC.sub.ks.sup.mC.sub.k 44.2 950 965 368 533452 .sup.mC.sub.esT.sub.esG.sub.es.sup.mC.sub.ds.sup.mC.sub.dsT.sub.dsG- .sub.dsT.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsA.sub.dsG.sub.dsG- .sub.ks.sup.mC.sub.ks.sup.mC.sub.k 55.6 951 966 369 533453 G.sub.es.sup.mC.sub.esT.sub.esG.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsT- .sub.dsG.sub.dsT.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsA.sub.dsG- .sub.ksG.sub.ks.sup.mC.sub.k 71.2 952 967 370 533454 G.sub.esG.sub.esT.sub.esG.sub.dsG.sub.ds.sup.mC.sub.dsA.sub.ds.sup.- mC.sub.ds.sup.mC.sub.dsT.sub.dsT.sub.ds.sup.mC.sub.dsG.sub.dsA.sub.ksA.sub- .ksA.sub.k 39.6 1276 1291 371 533455 .sup.mC.sub.esG.sub.esG.sub.esT.sub.dsG.sub.dsG.sub.ds.sup.mC.sub.d- sA.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsT.sub.dsT.sub.ds.sup.mC.sub.dsG.sub.k- sA.sub.ksA.sub.k 52.9 1277 1292 372 533456 T.sub.es.sup.mC.sub.esG.sub.esG.sub.dsT.sub.dsG.sub.dsG.sub.ds.sup.- mC.sub.dsA.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsT.sub.dsT.sub.ds.sup.mC.sub.k- sG.sub.ksA.sub.k 27 1278 1293 373 533457 A.sub.esG.sub.esT.sub.esG.sub.dsA.sub.dsG.sub.ds.sup.mC.sub.ds.sup.- mC.sub.ds.sup.mC.sub.dsG.sub.dsT.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsT.sub.k- s.sup.mC.sub.ks.sup.mC.sub.k 51.5 1315 1330 374 533458 .sup.mC.sub.esA.sub.esG.sub.esT.sub.dsG.sub.dsA.sub.dsG.sub.ds.sup.- mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsG.sub.dsT.sub.ds.sup.mC.sub.ds.sup.mC- .sub.ksT.sub.ks.sup.mC.sub.k 55.1 1316 1331 375 533459 G.sub.es.sup.mC.sub.esA.sub.esG.sub.dsT.sub.dsG.sub.dsA.sub.dsG.sub- .ds.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsG.sub.dsT.sub.ds.sup.mC.sub.k- s.sup.mC.sub.ksT.sub.k 33.7 1317 1332 376 533460 T.sub.es.sup.mC.sub.es.sup.mC.sub.es.sup.mC.sub.dsG.sub.dsA.sub.dsA- .sub.dsT.sub.dsG.sub.dsT.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsG.sub.dsA.sub.k- s.sup.mC.sub.ksA.sub.k 28.7 1344 1359 377 533461 T.sub.esT.sub.es.sup.mC.sub.es.sup.mC.sub.ds.sup.mC.sub.dsG.sub.dsA- .sub.dsA.sub.dsT.sub.dsG.sub.dsT.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsG.sub.k- sA.sub.ks.sup.mC.sub.k 36.2 1345 1360 378 533462 .sup.mC.sub.esT.sub.esT.sub.es.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.s- ub.dsG.sub.dsA.sub.dsA.sub.dsT.sub.dsG.sub.dsT.sub.ds.sup.mC.sub.ds.sup.mC- .sub.ksG.sub.ksA.sub.k 23 1346 1361 379 533463 .sup.mC.sub.es.sup.mC.sub.esT.sub.esT.sub.ds.sup.mC.sub.ds.sup.mC.s- ub.ds.sup.mC.sub.dsG.sub.dsA.sub.dsA.sub.dsT.sub.dsG.sub.dsT.sub.ds.sup.mC- .sub.ks.sup.mC.sub.ksG.sub.k 11.5 1347 1362 380 533464 A.sub.es.sup.mC.sub.es.sup.mC.sub.esT.sub.dsT.sub.ds.sup.mC.sub.ds.- sup.mC.sub.ds.sup.mC.sub.dsG.sub.dsA.sub.dsA.sub.dsT.sub.dsG.sub.dsT.sub.k- s.sup.mC.sub.ks.sup.mC.sub.k 19.9 1348 1363 381 533465 .sup.mC.sub.esA.sub.es.sup.mC.sub.es.sup.mC.sub.dsT.sub.dsT.sub.ds.- sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsG.sub.dsA.sub.dsA.sub.dsT.sub.dsG- .sub.ksT.sub.ks.sup.mC.sub.k 30.2 1349 1364 382 533466 G.sub.es.sup.mC.sub.esA.sub.es.sup.mC.sub.ds.sup.mC.sub.dsT.sub.dsT- .sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsG.sub.dsA.sub.dsA.sub.dsT- .sub.ksG.sub.ksT.sub.k 30.2 1350 1365 383 533467 .sup.mC.sub.esG.sub.es.sup.mC.sub.esA.sub.ds.sup.mC.sub.ds.sup.mC.s- ub.dsT.sub.dsT.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsG.sub.dsA.s- ub.dsA.sub.ksT.sub.ksG.sub.k 35.5 1351 1366 384 533468 A.sub.esT.sub.es.sup.mC.sub.es.sup.mC.sub.dsG.sub.ds.sup.mC.sub.dsT- .sub.ds.sup.mC.sub.ds.sup.mC.sub.dsT.sub.dsG.sub.ds.sup.mC.sub.dsA.sub.dsA- .sub.ks.sup.mC.sub.ksT.sub.k 47.4 1746 1761 385 533469 .sup.mC.sub.esA.sub.esT.sub.es.sup.mC.sub.ds.sup.mC.sub.dsG.sub.ds.- sup.mC.sub.dsT.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsT.sub.dsG.sub.ds.sup.mC.s- ub.dsA.sub.ksA.sub.ks.sup.mC.sub.k 51.2 1747 1762 386 533470 .sup.mC.sub.es.sup.mC.sub.esA.sub.esT.sub.ds.sup.mC.sub.ds.sup.mC.s- ub.dsG.sub.ds.sup.mC.sub.dsT.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsT.sub.dsG.s- ub.ds.sup.mC.sub.ksA.sub.ksA.sub.k 35.5 1748 1763 387 533471 G.sub.es.sup.mC.sub.esT.sub.es.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.s- ub.dsT.sub.ds.sup.mC.sub.dsT.sub.dsG.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsT.s- ub.dsG.sub.ks.sup.mC.sub.ksA.sub.k 65.6 1770 1785 388 533472 A.sub.esG.sub.esG.sub.esT.sub.dsG.sub.dsG.sub.dsA.sub.dsT.sub.ds.su- p.mC.sub.ds.sup.mC.sub.dsG.sub.dsT.sub.dsG.sub.dsG.sub.ks.sup.mC.sub.ks.su- p.mC.sub.k 51.8 1816 1831 389 533473 G.sub.esG.sub.esG.sub.esA.sub.dsA.sub.dsG.sub.dsG.sub.dsT.sub.dsG.s- ub.dsG.sub.dsA.sub.dsT.sub.ds.sup.mC.sub.ds.sup.mC.sub.ksG.sub.ksT.sub.k 44.9 1820 1835 390 533474 A.sub.es.sup.mC.sub.esA.sub.esG.sub.dsG.sub.dsA.sub.dsG.sub.ds.sup.- mC.sub.dsA.sub.dsG.sub.dsG.sub.dsG.sub.dsA.sub.dsA.sub.ksA.sub.ksG.sub.k 80.8 1955 1970 391 533475 .sup.mC.sub.esA.sub.esG.sub.esA.sub.ds.sup.mC.sub.dsT.sub.dsG.sub.d- s.sup.mC.sub.dsG.sub.dsG.sub.dsT.sub.dsG.sub.dsA.sub.dsG.sub.ksT.sub.ksT.s- ub.k 95.5 2034 2049 392 533476 G.sub.esG.sub.es.sup.mC.sub.esT.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsT- .sub.dsG.sub.dsG.sub.dsG.sub.ds.sup.mC.sub.dsG.sub.dsG.sub.ds.sup.mC.sub.k- sG.sub.ks.sup.mC.sub.k 55.7 2050 2065 393 533477 G.sub.esG.sub.es.sup.mC.sub.esG.sub.dsG.sub.ds.sup.mC.sub.dsT.sub.d- s.sup.mC.sub.ds.sup.mC.sub.dsT.sub.dsG.sub.dsG.sub.dsG.sub.ds.sup.mC.sub.k- sG.sub.ksG.sub.k 45.8 2053 2068 394 533478 .sup.mC.sub.esG.sub.es.sup.mC.sub.esG.sub.dsG.sub.dsG.sub.ds.sup.mC- .sub.dsG.sub.dsG.sub.ds.sup.mC.sub.dsT.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsT- .sub.ksG.sub.ksG.sub.k 83.7 2057 2072 395 533479 G.sub.esA.sub.esG.sub.es.sup.mC.sub.dsG.sub.ds.sup.mC.sub.dsG.sub.d- sG.sub.dsG.sub.ds.sup.mC.sub.dsG.sub.dsG.sub.ds.sup.mC.sub.dsT.sub.ks.sup.- mC.sub.ks.sup.mC.sub.k 79.8 2060 2075 396 533480 G.sub.esG.sub.esT.sub.esT.sub.ds.sup.mC.sub.dsA.sub.dsG.sub.dsG.sub- .dsG.sub.dsA.sub.dsG.sub.ds.sup.mC.sub.dsG.sub.ds.sup.mC.sub.ksG.sub.ksG.s- ub.k 49.4 2068 2083 397 533481 A.sub.esG.sub.esT.sub.esT.sub.ds.sup.mC.sub.dsT.sub.dsA.sub.dsG.sub- .dsG.sub.dsG.sub.dsT.sub.dsT.sub.ds.sup.mC.sub.dsA.sub.ksG.sub.ksG.sub.k 37 2076 2091 398 533482 .sup.mC.sub.esA.sub.esG.sub.esT.sub.dsT.sub.ds.sup.mC.sub.dsT.sub.d- sA.sub.dsG.sub.dsG.sub.dsG.sub.dsT.sub.dsT.sub.ds.sup.mC.sub.ksA.sub.ksG.s- ub.k 28.5 2077 2092 399 533483 A.sub.es.sup.mC.sub.esA.sub.esG.sub.dsT.sub.dsT.sub.ds.sup.mC.sub.d- sT.sub.dsA.sub.dsG.sub.dsG.sub.dsG.sub.dsT.sub.dsT.sub.ks.sup.mC.sub.ksA.s- ub.k 42 2078 2093 400

533484 G.sub.esA.sub.es.sup.mC.sub.esA.sub.dsG.sub.dsT.sub.dsT.sub.ds.sup.- mC.sub.dsT.sub.dsA.sub.dsG.sub.dsG.sub.dsG.sub.dsT.sub.ksT.sub.ks.sup.mC.s- ub.k 37.4 2079 2094 401 533485 A.sub.esG.sub.esA.sub.es.sup.mC.sub.dsA.sub.dsG.sub.dsT.sub.dsT.sub- .ds.sup.mC.sub.dsT.sub.dsA.sub.dsG.sub.dsG.sub.dsG.sub.ksT.sub.ksT.sub.k 66.5 2080 2095 402 533486 A.sub.esA.sub.esG.sub.esA.sub.ds.sup.mC.sub.dsA.sub.dsG.sub.dsT.sub- .dsT.sub.ds.sup.mC.sub.dsT.sub.dsA.sub.dsG.sub.dsG.sub.ksG.sub.ksT.sub.k 62.4 2081 2096 403 533487 G.sub.esA.sub.esA.sub.esG.sub.dsA.sub.ds.sup.mC.sub.dsA.sub.dsG.sub- .dsT.sub.dsT.sub.ds.sup.mC.sub.dsT.sub.dsA.sub.dsG.sub.ksG.sub.ksG.sub.k 56.9 2082 2097 404 533488 .sup.mC.sub.esG.sub.esA.sub.esA.sub.dsG.sub.dsA.sub.ds.sup.mC.sub.d- sA.sub.dsG.sub.dsT.sub.dsT.sub.ds.sup.mC.sub.dsT.sub.dsA.sub.ksG.sub.ksG.s- ub.k 36.8 2083 2098 405 533489 T.sub.es.sup.mC.sub.esG.sub.esA.sub.dsA.sub.dsG.sub.dsA.sub.ds.sup.- mC.sub.dsA.sub.dsG.sub.dsT.sub.dsT.sub.ds.sup.mC.sub.dsT.sub.ksA.sub.ksG.s- ub.k 49.6 2084 2099 406 533490 G.sub.esT.sub.es.sup.mC.sub.esG.sub.dsA.sub.dsA.sub.dsG.sub.dsA.sub- .ds.sup.mC.sub.dsA.sub.dsG.sub.dsT.sub.dsT.sub.ds.sup.mC.sub.ksT.sub.ksA.s- ub.k 40.4 2085 2100 407 533491 A.sub.esG.sub.esT.sub.es.sup.mC.sub.dsG.sub.dsA.sub.dsA.sub.dsG.sub- .dsA.sub.ds.sup.mC.sub.dsA.sub.dsG.sub.dsT.sub.dsT.sub.ks.sup.mC.sub.ksT.s- ub.k 37.4 2086 2101 408 533492 G.sub.esA.sub.esG.sub.esT.sub.ds.sup.mC.sub.dsG.sub.dsA.sub.dsA.sub- .dsG.sub.dsA.sub.ds.sup.mC.sub.dsA.sub.dsG.sub.dsT.sub.ksT.sub.ks.sup.mC.s- ub.k 36.6 2087 2102 409 533493 G.sub.esG.sub.esA.sub.esG.sub.dsT.sub.ds.sup.mC.sub.dsG.sub.dsA.sub- .dsA.sub.dsG.sub.dsA.sub.ds.sup.mC.sub.dsA.sub.dsG.sub.ksT.sub.ksT.sub.k 33.2 2088 2103 410 533494 .sup.mC.sub.esG.sub.esG.sub.esA.sub.dsG.sub.dsT.sub.ds.sup.mC.sub.d- sG.sub.dsA.sub.dsA.sub.dsG.sub.dsA.sub.ds.sup.mC.sub.dsA.sub.ksG.sub.ksT.s- ub.k 45.3 2089 2104 411 533495 .sup.mC.sub.es.sup.mC.sub.esG.sub.esG.sub.dsA.sub.dsG.sub.dsT.sub.d- s.sup.mC.sub.dsG.sub.dsA.sub.dsA.sub.dsG.sub.dsA.sub.ds.sup.mC.sub.ksA.sub- .ksG.sub.k 45.9 2090 2105 412 533496 .sup.mC.sub.es.sup.mC.sub.es.sup.mC.sub.esG.sub.dsG.sub.dsA.sub.dsG- .sub.dsT.sub.ds.sup.mC.sub.dsG.sub.dsA.sub.dsA.sub.dsG.sub.dsA.sub.ks.sup.- mC.sub.ksA.sub.k 51.3 2091 2106 413 533497 .sup.mC.sub.es.sup.mC.sub.es.sup.mC.sub.es.sup.mC.sub.dsG.sub.dsG.s- ub.dsA.sub.dsG.sub.dsT.sub.ds.sup.mC.sub.dsG.sub.dsA.sub.dsA.sub.dsG.sub.k- sA.sub.ks.sup.mC.sub.k 49.2 2092 2107 414 533498 G.sub.es.sup.mC.sub.es.sup.mC.sub.es.sup.mC.sub.ds.sup.mC.sub.dsG.s- ub.dsG.sub.dsA.sub.dsG.sub.dsT.sub.ds.sup.mC.sub.dsG.sub.dsA.sub.dsA.sub.k- sG.sub.ksA.sub.k 52.3 2093 2108 415 533499 G.sub.esG.sub.es.sup.mC.sub.es.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.s- ub.dsG.sub.dsG.sub.dsA.sub.dsG.sub.dsT.sub.ds.sup.mC.sub.dsG.sub.dsA.sub.k- sA.sub.ksG.sub.k 54.9 2094 2109 416 533500 G.sub.esG.sub.esG.sub.es.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.- sup.mC.sub.dsG.sub.dsG.sub.dsA.sub.dsG.sub.dsT.sub.ds.sup.mC.sub.dsG.sub.k- sA.sub.ksA.sub.k 46.7 2095 2110 417 533809 A.sub.es.sup.mC.sub.esA.sub.esA.sub.dsT.sub.dsA.sub.dsA.sub.dsA.sub- .dsT.sub.dsA.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsG.sub.dsA.sub.ksG.sub.ksG.s- ub.k 51.4 2773 2788 418

Example 4

Design of Antisense Oligonucleotides Targeting Human Dystrophia Myotonica Protein Kinase (hDMPK)

Dose Response HepG2

[0672] A series of antisense oligonucleotides (ASOs) were designed to target hDMPK. The newly designed ASOs were prepared using standard oligonucleotide synthesis well known in the art and are described in Table 8, below. Subscripts "s" indicate phosphorothioate internucleoside linkages; subscripts "k" indicate 6'-(S)--CH.sub.3 bicyclic nucleosides (cEt); subscripts "e" indicate 2'-O-methoxyethyl (MOE) modified nucleosides; and subscripts "d" indicate .beta.-D-2'-deoxyribonucleosides. ".sup.mC" indicates 5-methylcytosine nucleosides.

[0673] The antisense oligonucleotides are targeted to SEQ ID NO: 1 (GENBANK Accession No. NM_001081560.1). "Start site" indicates the 5'-most nucleoside to which the gapmer is targeted in the human gene sequence. "Stop site" indicates the 3'-most nucleoside to which the gapmer is targeted human gene sequence.

TABLE-US-00008 TABLE 8 Design of antisense oligonucleotides targeting hDMPK SEQ ISIS ID No. Composition (5' to 3') Start Site Stop Site NO 533440 G.sub.es.sup.mC.sub.es.sup.mC.sub.es.sup.mC.sub.dsA.sub.ds.sup.mC.s- ub.dsA.sub.dsG.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsT.sub.dsG.sub.ds.sup.mC.s- ub.dsA.sub.ksG.sub.ksG.sub.k 931 946 357 533442 .sup.mC.sub.es.sup.mC.sub.esG.sub.es.sup.mC.sub.ds.sup.mC.sub.ds.su- p.mC.sub.dsA.sub.ds.sup.mC.sub.dsA.sub.dsG.sub.ds.sup.mC.sub.ds.sup.mC.sub- .dsT.sub.dsG.sub.ks.sup.mC.sub.ksA.sub.k 933 948 359 533443 A.sub.es.sup.mC.sub.es.sup.mC.sub.esG.sub.ds.sup.mC.sub.ds.sup.mC.s- ub.ds.sup.mC.sub.dsA.sub.ds.sup.mC.sub.dsA.sub.dsG.sub.ds.sup.mC.sub.ds.su- p.mC.sub.dsT.sub.ksG.sub.ks.sup.mC.sub.k 934 949 360 533446 .sup.mC.sub.es.sup.mC.sub.es.sup.mC.sub.esA.sub.ds.sup.mC.sub.ds.su- p.mC.sub.dsG.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsA.sub.ds.sup.- mC.sub.dsA.sub.dsG.sub.ks.sup.mC.sub.ks.sup.mC.sub.k 937 952 363 533447 G.sub.es.sup.mC.sub.es.sup.mC.sub.es.sup.mC.sub.dsA.sub.ds.sup.mC.s- ub.ds.sup.mC.sub.dsG.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsA.sub- .ds.sup.mC.sub.dsA.sub.ksG.sub.ks.sup.mC.sub.k 938 953 364 533448 .sup.mC.sub.es.sup.mC.sub.esA.sub.esG.sub.dsG.sub.ds.sup.mC.sub.ds.- sup.mC.sub.ds.sup.mC.sub.dsA.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsG.sub.ds.su- p.mC.sub.ds.sup.mC.sub.ks.sup.mC.sub.ksA.sub.k 942 957 365 533449 .sup.mC.sub.es.sup.mC.sub.es.sup.mC.sub.esA.sub.dsG.sub.dsG.sub.ds.- sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsA.sub.ds.sup.mC.sub.ds.sup.mC.sub- .dsG.sub.ds.sup.mC.sub.ks.sup.mC.sub.ks.sup.mC.sub.k 943 958 366 533462 .sup.mC.sub.esT.sub.esT.sub.es.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.s- ub.dsG.sub.dsA.sub.dsA.sub.dsT.sub.dsG.sub.dsT.sub.ds.sup.mC.sub.ds.sup.mC- .sub.ksG.sub.ksA.sub.k 1346 1361 379 533463 .sup.mC.sub.es.sup.mC.sub.esT.sub.esT.sub.ds.sup.mC.sub.ds.sup.mC.s- ub.ds.sup.mC.sub.dsG.sub.dsA.sub.dsA.sub.dsT.sub.dsG.sub.dsT.sub.ds.sup.mC- .sub.ks.sup.mC.sub.ksG.sub.k 1347 1362 380 533464 A.sub.es.sup.mC.sub.es.sup.mC.sub.esT.sub.dsT.sub.ds.sup.mC.sub.ds.- sup.mC.sub.ds.sup.mC.sub.dsG.sub.dsA.sub.dsA.sub.dsT.sub.dsG.sub.dsT.sub.k- s.sup.mC.sub.ks.sup.mC.sub.k 1348 1363 381 533529 .sup.mC.sub.esG.sub.esG.sub.esT.sub.dsT.sub.dsG.sub.dsT.sub.dsG.sub- .dsA.sub.dsA.sub.ds.sup.mC.sub.dsT.sub.dsG.sub.dsG.sub.ks.sup.mC.sub.ksA.s- ub.k 2162 2177 23 533530 A.sub.esG.sub.es.sup.mC.sub.esG.sub.dsG.sub.dsT.sub.dsT.sub.dsG.sub- .dsT.sub.dsG.sub.dsA.sub.dsA.sub.ds.sup.mC.sub.dsT.sub.ksG.sub.ksG.sub.k 2164 2179 419 533599 G.sub.es.sup.mC.sub.esA.sub.es.sup.mC.sub.dsT.sub.dsT.sub.dsT.sub.d- sG.sub.ds.sup.mC.sub.dsG.sub.dsA.sub.dsA.sub.ds.sup.mC.sub.ds.sup.mC.sub.k- sA.sub.ksA.sub.k 2492 2507 420 533600 T.sub.esG.sub.es.sup.mC.sub.esA.sub.ds.sup.mC.sub.dsT.sub.dsT.sub.d- sT.sub.dsG.sub.ds.sup.mC.sub.dsG.sub.dsA.sub.dsA.sub.ds.sup.mC.sub.ks.sup.- mC.sub.ksA.sub.k 2493 2508 421

Example 5

Dose Response HepG2

[0674] Antisense oligonucleotides targeted to a human DMPK nucleic acid were tested for their effect on human DMPK RNA transcript in vitro. Cultured HepG2 cells at a density of 20,000 cells per well were transfected using electroporation with 625 nM, 1250 nM, 2500 nM, 5000 nM, and 10000.0 nM concentrations of each antisense oligonucleotide. After approximately 24 hours, RNA was isolated from the cells and DMPK RNA transcript levels were measured by quantitative real-time PCR using primer probe set RTS3164 (forward sequence AGCCTGAGCCGGGAGATG, designated herein as SEQ ID NO: 20; reverse sequence GCGTAGTTGACTGGCGAAGTT, designated herein as SEQ ID NO: 21; probe sequence AGGCCATCCGCACGGACAACCX, designated herein as SEQ ID NO: 22). Human DMPK RNA transcript levels were adjusted according to total RNA content, as measured by RIBOGREEN.RTM.. Results are presented in the table below as percent expression of human DMPK, relative to untreated control (UTC) cells. The tested antisense oligonucleotide sequences demonstrated dose-dependent inhibition of human DMPK mRNA levels under the conditions specified above.

TABLE-US-00009 TABLE 9 Inhibition of human DMPK RNA transcript in HepG2 cells targeting SEQ ID NO: 1 ISIS Dose % Target Start Site Stop Site No. (nM) Expression on Seq ID: 1 on Seq ID: 1 UTC N/A 100 N/A N/A 486178 625.0 39.4 2773 2788 486178 1250.0 31.2 2773 2788 486178 2500.0 20.6 2773 2788 486178 5000.0 13 2773 2788 486178 10000.0 11.5 2773 2788 533440 625.0 55.4 931 946 533440 1250.0 40.4 931 946 533440 2500.0 25.4 931 946 533440 5000.0 22.6 931 946 533440 10000.0 10.3 931 946 533442 625.0 55.2 933 948 533442 1250.0 33.1 933 948 533442 2500.0 29 933 948 533442 5000.0 16.9 933 948 533442 10000.0 7.2 933 948 533443 625.0 44.8 934 949 533443 1250.0 29.4 934 949 533443 2500.0 19.9 934 949 533443 5000.0 10.8 934 949 533443 10000.0 7 934 949 533446 625.0 50.9 937 952 533446 1250.0 35.5 937 952 533446 2500.0 30.4 937 952 533446 5000.0 14.6 937 952 533446 10000.0 14 937 952 533447 625.0 53.3 938 953 533447 1250.0 31.7 938 953 533447 2500.0 16.8 938 953 533447 5000.0 11.7 938 953 533447 10000.0 4.4 938 953 533448 625.0 58.8 942 957 533448 1250.0 36.9 942 957 533448 2500.0 24.8 942 957 533448 5000.0 11.5 942 957 533448 10000.0 10.1 942 957 533449 625.0 61.1 943 958 533449 1250.0 42.8 943 958 533449 2500.0 30.4 943 958 533449 5000.0 20.2 943 958 533449 10000.0 10.1 943 958 533462 625.0 50.7 1346 1361 533462 1250.0 32.3 1346 1361 533462 2500.0 29.2 1346 1361 533462 5000.0 12.5 1346 1361 533462 10000.0 5.8 1346 1361 533463 625.0 39.1 1347 1362 533463 1250.0 23.7 1347 1362 533463 2500.0 12.6 1347 1362 533463 5000.0 9.3 1347 1362 533463 10000.0 3.2 1347 1362 533464 625.0 48.8 1348 1363 533464 1250.0 36.4 1348 1363 533464 2500.0 24.5 1348 1363 533464 5000.0 11.7 1348 1363 533464 10000.0 5 1348 1363 533529 625.0 35.8 2162 2177 533529 1250.0 26.4 2162 2177 533529 2500.0 18.3 2162 2177 533529 5000.0 14.8 2162 2177 533529 10000.0 14.7 2162 2177 533530 625.0 47.4 2164 2179 533530 1250.0 22.1 2164 2179 533530 2500.0 21.5 2164 2179 533530 5000.0 14.4 2164 2179 533530 10000.0 8 2164 2179 533599 625.0 31.3 2492 2507 533599 1250.0 21.9 2492 2507 533599 2500.0 13.1 2492 2507 533599 5000.0 8.8 2492 2507 533599 10000.0 7.3 2492 2507 533600 625.0 33.8 2493 2508 533600 1250.0 20.9 2493 2508 533600 2500.0 16.5 2493 2508 533600 5000.0 10.4 2493 2508 533600 10000.0 12.1 2493 2508

Example 6

Design of Antisense Oligonucleotides Targeting Human Dystrophia Myotonica Protein Kinase (hDMPK)

[0675] A series of antisense oligonucleotides (ASOs) were designed to target hDMPK. The newly designed ASOs were prepared using standard oligonucleotide synthesis well known in the art and are described in Table 10, below. Subscripts "s" indicate phosphorothioate internucleoside linkages; subscripts "k" indicate 6'-(S)--CH.sub.3 bicyclic nucleosides (cEt); subscripts "e" indicate 2'-O-methoxyethyl (MOE) modified nucleosides; and subscripts "d" indicate .beta.-D-2'-deoxyribonucleosides. ".sup.mC" indicates 5-methylcytosine nucleosides.

[0676] The antisense oligonucleotides are targeted to SEQ ID NO: 2 (the complement of GENBANK Accession No. NT_011109.15 truncated from nucleotides 18540696 to Ser. No. 18/555,106). "Start site" indicates the 5'-most nucleoside to which the gapmer is targeted in the human gene sequence. "Stop site" indicates the 3'-most nucleoside to which the gapmer is targeted human gene sequence.

TABLE-US-00010 TABLE 10 Design of antisense oligonucleotides targeting hDMPK Start Site Stop Site Seq ISIS on Seq on Seq ID No. Sequence ID: 2 ID: 2 No. UTC N/A N/A N/A 486178 A.sub.ks.sup.mC.sub.ksA.sub.ksA.sub.dsT.sub.dsA.sub.dsA.sub.dsA.sub- .dsT.sub.dsA.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsG.sub.dsA.sub.ksG.sub.ksG.s- ub.k 13836 13851 23 533597 A.sub.es.sup.mC.sub.esT.sub.esT.sub.dsT.sub.dsG.sub.ds.sup.mC.sub.d- sG.sub.dsA.sub.dsA.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsA.sub.dsA.sub.ks.sup.- mC.sub.ksG.sub.k 13553 13568 422 533603 A.sub.esA.sub.esA.sub.esG.sub.ds.sup.mC.sub.dsT.sub.dsT.sub.dsT.sub- .dsG.sub.ds.sup.mC.sub.dsA.sub.ds.sup.mC.sub.dsT.sub.dsT.sub.ksT.sub.ksG.s- ub.k 13563 13578 423 533617 T.sub.es.sup.mC.sub.es.sup.mC.sub.es.sup.mC.sub.dsG.sub.dsA.sub.dsG- .sub.dsT.sub.dsA.sub.dsA.sub.dsG.sub.ds.sup.mC.sub.dsA.sub.dsG.sub.ksG.sub- .ks.sup.mC.sub.k 13624 13639 424 533649 G.sub.es.sup.mC.sub.esA.sub.esG.sub.ds.sup.mC.sub.dsG.sub.ds.sup.mC- .sub.dsA.sub.dsA.sub.dsG.sub.dsT.sub.dsG.sub.dsA.sub.dsG.sub.ksG.sub.ksA.s- ub.k 13686 13701 425 533694 G.sub.esT.sub.es.sup.mC.sub.esA.sub.dsG.sub.ds.sup.mC.sub.dsG.sub.d- sA.sub.dsG.sub.dsT.sub.ds.sup.mC.sub.dsG.sub.dsG.sub.dsA.sub.ksG.sub.ksG.s- ub.k 13760 13775 426 533697 .sup.mC.sub.es.sup.mC.sub.esT.sub.esG.sub.dsT.sub.ds.sup.mC.sub.dsA- .sub.dsG.sub.ds.sup.mC.sub.dsG.sub.dsA.sub.dsG.sub.dsT.sub.ds.sup.mC.sub.k- sG.sub.ksG.sub.k 13763 13778 427 533698 G.sub.es.sup.mC.sub.es.sup.mC.sub.esT.sub.dsG.sub.dsT.sub.ds.sup.mC- .sub.dsA.sub.dsG.sub.ds.sup.mC.sub.dsG.sub.dsA.sub.dsG.sub.dsT.sub.ks.sup.- mC.sub.ksG.sub.k 13764 13779 428 533699 A.sub.esG.sub.es.sup.mC.sub.es.sup.mC.sub.dsT.sub.dsG.sub.dsT.sub.d- s.sup.mC.sub.dsA.sub.dsG.sub.ds.sup.mC.sub.dsG.sub.dsA.sub.dsG.sub.ksT.sub- .ks.sup.mC.sub.k 13765 13780 429 533711 G.sub.esG.sub.esG.sub.esT.sub.ds.sup.mC.sub.dsT.sub.ds.sup.mC.sub.d- sA.sub.dsG.sub.dsT.sub.dsG.sub.ds.sup.mC.sub.dsA.sub.dsT.sub.ks.sup.mC.sub- .ks.sup.mC.sub.k 13813 13828 430 533721 A.sub.esG.sub.esG.sub.esT.sub.dsT.sub.dsT.sub.dsT.sub.dsT.sub.ds.su- p.mC.sub.ds.sup.mC.sub.dsA.sub.dsG.sub.dsA.sub.dsG.sub.ksG.sub.ks.sup.mC.s- ub.k 2580 2595 431 533722 A.sub.esA.sub.esG.sub.esG.sub.dsT.sub.dsT.sub.dsT.sub.dsT.sub.dsT.s- ub.ds.sup.mC.sub.ds.sup.mC.sub.dsA.sub.dsG.sub.dsA.sub.ksG.sub.ksG.sub.k 2581 2596 432 533751 G.sub.esG.sub.esT.sub.es.sup.mC.sub.dsA.sub.ds.sup.mC.sub.dsT.sub.d- sG.sub.ds.sup.mC.sub.dsT.sub.dsG.sub.dsG.sub.dsG.sub.dsT.sub.ks.sup.mC.sub- .ks.sup.mC.sub.k 6446 6461 433 533786 G.sub.esT.sub.esG.sub.esG.sub.dsT.sub.dsT.sub.dsT.sub.ds.sup.mC.sub- .dsT.sub.dsG.sub.dsT.sub.ds.sup.mC.sub.dsT.sub.dsG.sub.ks.sup.mC.sub.ksT.s- ub.k 11099 11114 434 533787 .sup.mC.sub.esG.sub.esT.sub.esG.sub.dsG.sub.dsT.sub.dsT.sub.dsT.sub- .ds.sup.mC.sub.dsT.sub.dsG.sub.dsT.sub.ds.sup.mC.sub.dsT.sub.ksG.sub.ks.su- p.mC.sub.k 11100 11115 435

Example 7

Dose Response for ASOs Targeted to a Human DMPK RNA Transcript in HepG2 Cells

[0677] Antisense oligonucleotides targeted to a human DMPK nucleic acid were tested for their effect on human DMPK RNA transcript in vitro. Cultured HepG2 cells at a density of 20,000 cells per well were transfected using electroporation with 625 nM, 1250 nM, 2500 nM, 5000 nM, and 10000.0 nM concentrations of each antisense oligonucleotide. After approximately 24 hours, RNA was isolated from the cells and DMPK RNA transcript levels were measured by quantitative real-time PCR using primer probe set RTS3164 (forward sequence AGCCTGAGCCGGGAGATG, designated herein as SEQ ID NO: 20; reverse sequence GCGTAGTTGACTGGCGAAGTT, designated herein as SEQ ID NO: 21; probe sequence AGGCCATCCGCACGGACAACCX, designated herein as SEQ ID NO: 22). Human DMPK RNA transcript levels were adjusted according to total RNA content, as measured by RIBOGREEN.RTM.. Results are presented as percent expression of human DMPK, relative to untreated control (UTC) cells and are shown in the table below. The tested antisense oligonucleotide sequences demonstrated dose-dependent inhibition of human DMPK mRNA levels under the conditions specified above.

TABLE-US-00011 TABLE 11 Inhibition of human DMPK RNA transcript in HepG2 cells targeting SEQ ID NO: 1 ISIS Dose % Target Start Site Stop Site on No. (nM) Expression on Seq ID: 2 Seq ID: 2 UTC NA 100 N/A N/A 486178 625.000 39.4 13836 13851 486178 1250.000 27.3 13836 13851 486178 2500.000 14 13836 13851 486178 5000.000 16.3 13836 13851 486178 10000.000 8.3 13836 13851 533597 625.000 42.4 13553 13568 533597 1250.000 30.3 13553 13568 533597 2500.000 15.3 13553 13568 533597 5000.000 10 13553 13568 533597 10000.000 10.6 13553 13568 533603 625.000 48.2 13563 13578 533603 1250.000 31.1 13563 13578 533603 2500.000 22.4 13563 13578 533603 5000.000 15.6 13563 13578 533603 10000.000 9.9 13563 13578 533617 625.000 38.4 13624 13639 533617 1250.000 26.3 13624 13639 533617 2500.000 21.6 13624 13639 533617 5000.000 15.8 13624 13639 533617 10000.000 14.6 13624 13639 533649 625.000 52.2 13686 13701 533649 1250.000 27.8 13686 13701 533649 2500.000 24.6 13686 13701 533649 5000.000 20.5 13686 13701 533649 10000.000 14.5 13686 13701 533694 625.000 53.3 13760 13775 533694 1250.000 29.4 13760 13775 533694 2500.000 23.6 13760 13775 533694 5000.000 18.7 13760 13775 533694 10000.000 13.5 13760 13775 533697 625.000 30.6 13763 13778 533697 1250.000 14.9 13763 13778 533697 2500.000 13.8 13763 13778 533697 5000.000 9.7 13763 13778 533697 10000.000 7.1 13763 13778 533698 625.000 23.4 13764 13779 533698 1250.000 15.5 13764 13779 533698 2500.000 13.8 13764 13779 533698 5000.000 12.4 13764 13779 533698 10000.000 10.2 13764 13779 533699 625.000 38.2 13765 13780 533699 1250.000 26.9 13765 13780 533699 2500.000 17.6 13765 13780 533699 5000.000 12.9 13765 13780 533699 10000.000 9.3 13765 13780 533711 625.000 35.1 13813 13828 533711 1250.000 34.6 13813 13828 533711 2500.000 22.4 13813 13828 533711 5000.000 22 13813 13828 533711 10000.000 13 13813 13828 533721 625.000 36.3 2580 2595 533721 1250.000 29.8 2580 2595 533721 2500.000 23.2 2580 2595 533721 5000.000 17.8 2580 2595 533721 10000.000 17.2 2580 2595 533722 625.000 48.5 2581 2596 533722 1250.000 28.6 2581 2596 533722 2500.000 21.9 2581 2596 533722 5000.000 28.1 2581 2596 533722 10000.000 13.8 2581 2596 533751 625.000 37.7 6446 6461 533751 1250.000 21.6 6446 6461 533751 2500.000 12.6 6446 6461 533751 5000.000 9.7 6446 6461 533751 10000.000 8.5 6446 6461 533786 625.000 53.6 11099 11114 533786 1250.000 26.6 11099 11114 533786 2500.000 14.7 11099 11114 533786 5000.000 9.6 11099 11114 533786 10000.000 5.5 11099 11114 533787 625.000 43.8 11100 11115 533787 1250.000 27.7 11100 11115 533787 2500.000 16.3 11100 11115 533787 5000.000 7 11100 11115 533787 10000.000 4.5 11100 11115

Example 8

ASOs Designed to Target a Human DMPK RNA Transcript

[0678] A series of antisense oligonucleotides (ASOs) were designed to target hDMPK. The newly designed ASOs were prepared using standard oligonucleotide synthesis well known in the art and are described in Table 12, below. Subscripts "s" indicate phosphorothioate internucleoside linkages; subscripts "k" indicate 6'-(S)--CH.sub.3 bicyclic nucleosides (cEt); subscripts "e" indicate 2'-O-methoxyethyl (MOE) modified nucleosides; and subscripts "d" indicate .beta.-D-2'-deoxyribonucleosides. ".sup.mC" indicates 5-methylcytosine nucleosides.

[0679] The antisense oligonucleotides targeted to a human DMPK nucleic acid were tested for their effect on DMPK RNA transcript in vitro. Cultured hSKMC cells at a density of 20,000 cells per well were transfected using electroporation with 800 nM antisense oligonucleotide. After approximately 24 hours, RNA was isolated from the cells and DMPK transcript levels were measured by quantitative real-time PCR. DMPK RNA transcript levels were adjusted according to total RNA content, as measured by RIBOGREEN.RTM.. Results are presented as percent expression of DMPK, relative to untreated control cells.

[0680] `Target start site` indicates the 5'-most nucleoside to which the antisense oligonucleotide is targeted in the human genomic gene sequence. `Target stop site` indicates the 3'-most nucleoside to which the antisense oligonucleotide is targeted in the human genomic sequence. All the antisense oligonucleotides listed in Table 12 target SEQ ID NO: 1 (GENBANK Accession No. NM_001081560.1).

[0681] Several of the antisense oligonucleotides demonstrated significant inhibition of DMPK mRNA levels under the conditions specified above.

TABLE-US-00012 TABLE 12 Inhibition of human DMPK RNA transcript in HepG2 cells using ASOs targeting SEQ ID NO: 1 Start Site Stop Site Seq ISIS % Target on Seq on Seq ID No. Sequence Expression ID: 1 ID: 1 No. UTC N/A 100 N/A N/A 444401 T.sub.esT.sub.esG.sub.es.sup.mC.sub.esA.sub.es.sup.mC.sub.dsT.sub.d- sT.sub.dsT.sub.dsG.sub.ds.sup.mC.sub.dsG.sub.dsA.sub.dsA.sub.ds.sup.mC.sub- .ds.sup.mC.sub.esA.sub.esA.sub.es.sup.mC.sub.esG.sub.e 25.2 2490 2509 33 444436 G.sub.esT.sub.es.sup.mC.sub.esG.sub.esG.sub.esA.sub.dsG.sub.dsG.sub- .dsA.sub.ds.sup.mC.sub.dsG.sub.dsA.sub.dsG.sub.dsG.sub.dsT.sub.ds.sup.mC.s- ub.esA.sub.esA.sub.esT.sub.esA.sub.e 30.8 2685 2704 264 486072 A.sub.ksA.sub.ksGkSA.sub.ds.sup.mC.sub.dsA.sub.dsG.sub.dsT.sub.dsT.- sub.ds.sup.mC.sub.dsT.sub.dsA.sub.dsG.sub.dsG.sub.ksG.sub.ksT.sub.k 36.8 2081 2096 403 486073 .sup.mC.sub.ksG.sub.ksA.sub.ksA.sub.dsG.sub.dsA.sub.ds.sup.mC.sub.d- sA.sub.dsG.sub.dsT.sub.dsT.sub.ds.sup.mC.sub.dsT.sub.dsA.sub.ksG.sub.ksG.s- ub.k 22.4 2083 2098 405 486075 G.sub.ksT.sub.ks.sup.mC.sub.ksG.sub.dsA.sub.dsA.sub.dsG.sub.dsA.sub- .ds.sup.mC.sub.dsA.sub.dsG.sub.dsT.sub.dsT.sub.ds.sup.mC.sub.ksT.sub.ksA.s- ub.k 41.3 2085 2100 407 486076 A.sub.ksG.sub.ksT.sub.ks.sup.mC.sub.dsG.sub.dsA.sub.dsA.sub.dsG.sub- .dsA.sub.ds.sup.mC.sub.dsA.sub.dsG.sub.dsT.sub.dsT.sub.ks.sup.mC.sub.ksT.s- ub.k 22.4 2086 2101 408 486077 G.sub.ksA.sub.ksG.sub.ksT.sub.ds.sup.mC.sub.dsG.sub.dsA.sub.dsA.sub- .dsG.sub.dsA.sub.ds.sup.mC.sub.dsA.sub.dsG.sub.dsT.sub.ksT.sub.ks.sup.mC.s- ub.k 35.2 2087 2102 409 486078 .sup.mC.sub.ksG.sub.ksG.sub.ksA.sub.dsG.sub.dsT.sub.ds.sup.mC.sub.d- sG.sub.dsA.sub.dsA.sub.dsG.sub.dsA.sub.ds.sup.mC.sub.dsA.sub.ksG.sub.ksT.s- ub.k 12.4 2089 2104 411 486079 .sup.mC.sub.ks.sup.mC.sub.ks.sup.mC.sub.ksG.sub.dsG.sub.dsA.sub.dsG- .sub.dsT.sub.ds.sup.mC.sub.dsG.sub.dsA.sub.dsA.sub.dsG.sub.dsA.sub.ks.sup.- mC.sub.ksA.sub.k 36.5 2091 2106 413 486080 .sup.mC.sub.ks.sup.mC.sub.ks.sup.mC.sub.ks.sup.mC.sub.dsG.sub.dsG.s- ub.dsA.sub.dsG.sub.dsT.sub.ds.sup.mC.sub.dsG.sub.dsA.sub.dsA.sub.dsG.sub.k- sA.sub.ks.sup.mC.sub.k 19.9 2092 2107 414 486085 G.sub.ksA.sub.ksA.sub.ks.sup.mC.sub.dsT.sub.dsG.sub.dsG.sub.ds.sup.- mC.sub.dsA.sub.dsG.sub.dsG.sub.ds.sup.mC.sub.dsG.sub.dsG.sub.ksT.sub.ksG.s- ub.k 30.1 2155 2170 436 486086 T.sub.ksG.sub.ksT.sub.ksG.sub.dsA.sub.dsA.sub.dsr.sup.mC.sub.dsT.su- b.dsG.sub.dsG.sub.ds.sup.mC.sub.dsA.sub.dsG.sub.dsG.sub.ks.sup.mC.sub.ksG.- sub.k 17.2 2158 2173 437 486087 G.sub.ksG.sub.ksT.sub.ksT.sub.dsG.sub.dsT.sub.dsG.sub.dsA.sub.dsA.s- ub.ds.sup.mC.sub.dsT.sub.dsG.sub.dsG.sub.ds.sup.mC.sub.ksA.sub.ksG.sub.k 11.5 2161 2176 438 486088 G.sub.ksA.sub.ksG.sub.ks.sup.mC.sub.dsG.sub.dsG.sub.dsT.sub.dsT.sub- .dsG.sub.dsT.sub.dsG.sub.dsA.sub.dsA.sub.ds.sup.mC.sub.ksT.sub.ksG.sub.k 21.7 2165 2180 439 486094 A.sub.ks.sup.mC.sub.ksT.sub.ksG.sub.dsG.sub.dsA.sub.dsG.sub.ds.sup.- mC.sub.dsT.sub.dsG.sub.dsG.sub.dsG.sub.ds.sup.mC.sub.dsG.sub.ksG.sub.ksA.s- ub.k 30.2 2193 2208 440 486096 A.sub.ksG.sub.ksG.sub.ksA.sub.ds.sup.mC.sub.dsT.sub.dsG.sub.dsG.sub- .dsA.sub.dsG.sub.ds.sup.mC.sub.dsT.sub.dsG.sub.dsG.sub.ksG.sub.ks.sup.mC.s- ub.k 43.5 2196 2211 441 486097 T.sub.ks.sup.mC.sub.ksA.sub.ks.sup.mC.sub.dsA.sub.dsG.sub.dsG.sub.d- sA.sub.ds.sup.mC.sub.dsT.sub.dsG.sub.dsG.sub.dsA.sub.dsG.sub.ks.sup.mC.sub- .ksT.sub.k 54.5 2200 2215 442 486098 A.sub.ksT.sub.ks.sup.mC.sub.ksA.sub.ds.sup.mC.sub.dsA.sub.dsG.sub.d- sG.sub.dsA.sub.ds.sup.mC.sub.dsT.sub.dsG.sub.dsG.sub.dsA.sub.ksG.sub.ks.su- p.mC.sub.k 77.3 2201 2216 443 486099 G.sub.ksG.sub.ksA.sub.ksT.sub.ds.sup.mC.sub.dsA.sub.ds.sup.mC.sub.d- sA.sub.dsG.sub.dsG.sub.dsA.sub.ds.sup.mC.sub.dsT.sub.dsG.sub.ksG.sub.ksA.s- ub.k 24.8 2203 2218 444 486101 .sup.mC.sub.ksA.sub.ksG.sub.ks.sup.mC.sub.ds.sup.mC.sub.dsT.sub.dsG- .sub.dsG.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsG.sub.dsA.sub.dsA.sub.dsA.sub.k- sG.sub.ksA.sub.k 31.6 2386 2401 445 486102 .sup.mC.sub.ksT.sub.ks.sup.mC.sub.ksA.sub.dsG.sub.ds.sup.mC.sub.ds.- sup.mC.sub.dsT.sub.dsG.sub.dsG.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsG.sub.dsA- .sub.ksA.sub.ksA.sub.k 35.1 2388 2403 446 486104 G.sub.ksT.sub.ks.sup.mC.sub.ksA.sub.dsG.sub.dsG.sub.dsG.sub.ds.sup.- mC.sub.ds.sup.mC.sub.dsT.sub.ds.sup.mC.sub.dsA.sub.dsG.sub.ds.sup.mC.sub.k- s.sup.mC.sub.ksT.sub.k 26.9 2396 2411 447 486105 .sup.mC.sub.ksG.sub.ksT.sub.ks.sup.mC.sub.dsA.sub.dsG.sub.dsG.sub.d- sG.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsT.sub.ds.sup.mC.sub.dsA.sub.dsG.sub.k- s.sup.mC.sub.ks.sup.mC.sub.k 48.4 2397 2412 448 486110 T.sub.ksT.sub.ksT.sub.ksG.sub.ds.sup.mC.sub.dsA.sub.ds.sup.mC.sub.d- sT.sub.dsT.sub.dsT.sub.dsG.sub.ds.sup.mC.sub.dsG.sub.dsA.sub.ksA.sub.ks.su- p.mC.sub.k 31.6 2495 2510 449 486111 G.sub.ksA.sub.ksA.sub.ksA.sub.dsG.sub.ds.sup.mC.sub.dsT.sub.dsT.sub- .dsT.sub.dsG.sub.ds.sup.mC.sub.dsA.sub.ds.sup.mC.sub.dsT.sub.ksT.sub.ksT.s- ub.k 31.9 2501 2516 450 486112 A.sub.ksA.sub.ksT.sub.ksT.sub.dsT.sub.ds.sup.mC.sub.ds.sup.mC.sub.d- s.sup.mC.sub.dsG.sub.dsA.sub.dsG.sub.dsT.sub.dsA.sub.dsA.sub.ksG.sub.ks.su- p.mC.sub.k 47.4 2565 2580 451 486115 G.sub.ks.sup.mC.sub.ksA.sub.ksA.sub.dsA.sub.dsT.sub.dsT.sub.dsT.sub- .ds.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsG.sub.dsA.sub.dsG.sub.ksT.sub- .ksA.sub.k 20.8 2568 2583 452 486116 A.sub.ksG.sub.ks.sup.mC.sub.ksA.sub.dsA.sub.dsA.sub.dsT.sub.dsT.sub- .dsT.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsGdA.sub.ksG.sub.ksT.s- ub.k 23.9 2569 2584 453 486117 A.sub.ksA.sub.ksG.sub.ks.sup.mC.sub.dsA.sub.dsA.sub.dsA.sub.dsT.sub- .dsT.sub.dsT.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsG.sub.ksA.sub- .ksG.sub.k 22 2570 2585 454 486118 A.sub.ksA.sub.ksA.sub.ksG.sub.ds.sup.mC.sub.dsA.sub.dsA.sub.dsA.sub- .dsT.sub.dsT.sub.dsT.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.ksG.sub- .ksA.sub.k 26.7 2571 2586 455 486119 A.sub.ksA.sub.ksA.sub.ksA.sub.dsG.sub.ds.sup.mC.sub.dsAdA.sub.dsA.s- ub.dsT.sub.dsT.sub.dsT.sub.ds.sup.mC.sub.ds.sup.mC.sub.ks.sup.mC.sub.ksG.s- ub.k 33.5 2572 2587 456 486120 G.sub.ks.sup.mC.sub.ksA.sub.ksA.sub.dsA.sub.dsA.sub.dsG.sub.ds.sup.- mC.sub.dsA.sub.dsA.sub.dsA.sub.dsT.sub.dsT.sub.dsT.sub.ks.sup.mC.sub.ks.su- p.mC.sub.k 51.4 2574 2589 457 486121 G.sub.ksG.sub.ks.sup.mC.sub.ksAdA.sub.dsA.sub.dsA.sub.dsG.sub.ds.su- p.mC.sub.dsA.sub.dsA.sub.dsA.sub.dsT.sub.dsT.sub.ksT.sub.ks.sup.mC.sub.k 60.8 2575 2590 458 486123 T.sub.ksG.sub.ksG.sub.ds.sup.mC.sub.dsA.sub.dsA.sub.dsA.sub.dsA.sub- .dsG.sub.ds.sup.mC.sub.dsA.sub.dsA.sub.dsA.sub.ksT.sub.ksT.sub.k 39.8 2577 2592 459 486125 G.sub.ksT.sub.ksT.sub.ksT.sub.dsG.sub.dsG.sub.ds.sup.mC.sub.dsA.sub- .dsA.sub.dsA.sub.dsA.sub.dsG.sub.ds.sup.mC.sub.dsA.sub.ksA.sub.ksA.sub.k 32.7 2579 2594 460 486126 G.sub.ksG.sub.ksT.sub.ksT.sub.dsT.sub.dsG.sub.dsG.sub.ds.sup.mC.sub- .dsA.sub.dsA.sub.dsA.sub.dsA.sub.dsG.sub.ds.sup.mC.sub.ksA.sub.ksA.sub.k 19.2 2580 2595 461 486127 G.sub.ksG.sub.ksG.sub.ksT.sub.dsT.sub.dsT.sub.dsG.sub.dsG.sub.ds.su- p.mC.sub.dsA.sub.dsA.sub.dsA.sub.dsA.sub.dsG.sub.ks.sup.mC.sub.ksA.sub.k 36.1 2581 2596 462 486128 G.sub.ks.sup.mC.sub.ksG.sub.ksG.sub.dsG.sub.dsT.sub.dsT.sub.dsT.sub- .dsG.sub.dsG.sub.ds.sup.mC.sub.dsA.sub.dsA.sub.dsA.sub.ksA.sub.ksG.sub.k 39.1 2583 2598 463 486129 A.sub.ksG.sub.ks.sup.mC.sub.ksG.sub.dsG.sub.dsG.sub.dsT.sub.dsT.sub- .dsT.sub.dsG.sub.dsG.sub.ds.sup.mC.sub.dsA.sub.dsA.sub.ksA.sub.ksA.sub.k 31.4 2584 2599 464 486130 A.sub.ksA.sub.ksG.sub.ks.sup.mC.sub.dsG.sub.dsG.sub.dsG.sub.dsT.sub- .dsT.sub.dsT.sub.dsG.sub.dsG.sub.ds.sup.mC.sub.dsA.sub.ksA.sub.ksA.sub.k 35.7 2585 2600 465 486133 .sup.mC.sub.ksT.sub.ks.sup.mC.sub.ks.sup.mC.sub.dsG.sub.dsA.sub.dsG- .sub.dsA.sub.dsG.sub.ds.sup.mC.sub.dsA.sub.dsG.sub.ds.sup.mC.sub.dsG.sub.k- s.sup.mC.sub.ksA.sub.k 45.9 2631 2646 466 486134 G.sub.ks.sup.mC.sub.ksT.sub.ks.sup.mC.sub.ds.sup.mC.sub.dsG.sub.dsA- .sub.dsG.sub.dsA.sub.dsG.sub.ds.sup.mC.sub.dsA.sub.dsG.sub.ds.sup.mC.sub.k- sG.sub.ks.sup.mC.sub.k 29.5 2632 2647 467 486135 G.sub.ksG.sub.ks.sup.mC.sub.ksT.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsG- .sub.dsA.sub.dsG.sub.dsA.sub.dsG.sub.ds.sup.mC.sub.dsA.sub.dsG.sub.ks.sup.- mC.sub.ksG.sub.k 51.4 2633 2648 468 486142 T.sub.ksA.sub.ksA.sub.ksA.sub.dsT.sub.dsA.sub.dsT.sub.ds.sup.mC.sub- .ds.sup.mC.sub.dsAdA.sub.dsA.sub.ds.sup.mC.sub.ds.sup.mC.sub.ksG.sub.ks.su- p.mC.sub.k 64.4 2671 2686 469 486147 G.sub.ksT.sub.ks.sup.mC.sub.ksA.sub.dsA.sub.dsT.sub.dsA.sub.dsA.sub- .dsA.sub.dsT.sub.dsA.sub.dsT.sub.ds.sup.mC.sub.ds.sup.mC.sub.ksA.sub.ksA.s- ub.k 16.1 2676 2691 470 486148 A.sub.ksG.sub.ksG.sub.ksT.sub.ds.sup.mC.sub.dsA.sub.dsA.sub.dsT.sub- .dsA.sub.dsA.sub.dsA.sub.dsT.sub.dsA.sub.dsT.sub.ks.sup.mC.sub.ks.sup.mC.s- ub.k 18.3 2678 2693 471 486149 .sup.mC.sub.ksG.sub.ksA.sub.ksG.sub.dsG.sub.dsT.sub.ds.sup.mC.sub.d- sA.sub.dsA.sub.dsT.sub.dsA.sub.dsA.sub.dsA.sub.dsT.sub.ksA.sub.ksT.sub.k 37.9 2680 2695 472 486150 A.sub.ks.sup.mC.sub.ksG.sub.ksA.sub.dsG.sub.dsG.sub.dsT.sub.ds.sup.- mC.sub.dsA.sub.dsA.sub.dsT.sub.dsA.sub.dsA.sub.dsA.sub.ksT.sub.ksk 45.3 2681 2696 473 486151 G.sub.ksA.sub.ks.sup.mC.sub.ksG.sub.dsA.sub.dsG.sub.dsG.sub.dsT.sub- .ds.sup.mC.sub.dsA.sub.dsA.sub.dsT.sub.dsA.sub.dsA.sub.ksA.sub.ksT.sub.k 52.2 2682 2697 474 486152 G.sub.ksG.sub.ksA.sub.ks.sup.mC.sub.dsG.sub.dsA.sub.dsG.sub.dsG.sub- .dsT.sub.ds.sup.mC.sub.dsA.sub.dsA.sub.dsT.sub.dsA.sub.ksA.sub.ksA.sub.k 19.8 2683 2698 475 486153 A.sub.ksG.sub.ksG.sub.ksA.sub.ds.sup.mC.sub.dsG.sub.dsA.sub.dsG.sub- .dsG.sub.dsT.sub.ds.sup.mC.sub.dsA.sub.dsA.sub.dsT.sub.ksA.sub.ksA.sub.k 19.9 2684 2699 476 486154 G.sub.ksA.sub.ksG.sub.ksG.sub.dsA.sub.ds.sup.mC.sub.dsG.sub.dsA.sub- .dsG.sub.dsG.sub.dsT.sub.ds.sup.mC.sub.dsA.sub.dsA.sub.ksT.sub.ksA.sub.k 19.6 2685 2700 477 486155 G.sub.ksG.sub.ksA.sub.ksG.sub.dsG.sub.dsA.sub.ds.sup.mC.sub.dsG.sub- .dsA.sub.dsG.sub.dsG.sub.dsT.sub.ds.sup.mC.sub.dsA.sub.ksA.sub.ksT.sub.k 38.3 2686 2701 478 486156 .sup.mC.sub.ksG.sub.ksG.sub.ksA.sub.dsG.sub.dsG.sub.dsA.sub.ds.sup.- mC.sub.dsG.sub.dsA.sub.dsG.sub.dsG.sub.dsT.sub.ds.sup.mC.sub.ksA.sub.ksA.s- ub.k 14.1 2687 2702 479 486157 T.sub.ks.sup.mC.sub.ksG.sub.ksG.sub.dsA.sub.dsG.sub.dsG.sub.dsA.sub- .ds.sup.mC.sub.dsG.sub.dsA.sub.dsG.sub.dsG.sub.dsT.sub.ks.sup.mC.sub.ksA.s- ub.k 23.2 2688 2703 480 486158 G.sub.ksT.sub.ksC.sub.ksG.sub.dsG.sub.dsA.sub.dsG.sub.dsGdA.sub.ds.- sup.mC.sub.dsG.sub.dsA.sub.dsG.sub.dsG.sub.ksT.sub.ks.sup.mC.sub.k 34.5 2689 2704 481 486159 A.sub.ksG.sub.ksT.sub.ks.sup.mC.sub.dsG.sub.dsG.sub.dsA.sub.dsG.sub- .dsGdA.sub.ds.sup.mC.sub.dsG.sub.dsA.sub.dsG.sub.ksG.sub.ksT.sub.k 23.7 2690 2705 482 486160 G.sub.ksA.sub.ksG.sub.ksT.sub.ds.sup.mC.sub.dsG.sub.dsG.sub.dsA.sub- .dsG.sub.dsG.sub.dsA.sub.ds.sup.mC.sub.dsG.sub.dsA.sub.ksG.sub.ksG.sub.k 14.3 2691 2706 483 486161 .sup.mC.sub.ksG.sub.ksA.sub.ksG.sub.dsT.sub.ds.sup.mC.sub.dsG.sub.d- sG.sub.dsA.sub.dsG.sub.dsG.sub.dsA.sub.ds.sup.mC.sub.dsG.sub.ksA.sub.ksG.s- ub.k 29 2692 2707 484 486162 A.sub.ksG.sub.ks.sup.mC.sub.ksG.sub.dsA.sub.dsG.sub.dsT.sub.ds.sup.- mC.sub.dsG.sub.dsG.sub.dsA.sub.dsG.sub.dsG.sub.dsA.sub.ks.sup.mC.sub.ksG.s- ub.k 20.6 2694 2709 485

486163 .sup.mC.sub.ksA.sub.ksG.sub.ks.sup.mC.sub.dsG.sub.dsA.sub.dsG.sub.d- sT.sub.ds.sup.mC.sub.dsG.sub.dsG.sub.dsA.sub.dsG.sub.dsG.sub.ksA.sub.ks.su- p.mC.sub.k 29 2695 2710 486 486164 T.sub.ks.sup.mC.sub.ksA.sub.ksG.sub.ds.sup.mC.sub.dsG.sub.dsA.sub.d- sG.sub.dsT.sub.ds.sup.mC.sub.dsG.sub.dsG.sub.dsA.sub.dsG.sub.ksG.sub.ksA.s- ub.k 17 2696 2711 487 486165 G.sub.ksT.sub.ks.sup.mC.sub.ksA.sub.dsG.sub.ds.sup.mC.sub.dsG.sub.d- sA.sub.dsG.sub.dsT.sub.ds.sup.mC.sub.dsG.sub.dsG.sub.dsA.sub.ksG.sub.ksG.s- ub.k 14.2 2697 2712 426 486166 T.sub.ksG.sub.ksT.sub.ks.sup.mC.sub.dsA.sub.dsG.sub.ds.sup.mC.sub.d- sG.sub.dsA.sub.dsG.sub.dsT.sub.ds.sup.mC.sub.dsG.sub.dsG.sub.ksA.sub.ksG.s- ub.k 25.1 2698 2713 488 486167 .sup.mC.sub.ksT.sub.ksGkT.sub.ds.sup.mC.sub.dsA.sub.dsG.sub.ds.sup.- mC.sub.dsG.sub.dsA.sub.dsG.sub.dsT.sub.ds.sup.mC.sub.dsG.sub.ksG.sub.ksA.s- ub.k 15 2699 2714 489 486168 .sup.mC.sub.ks.sup.mC.sub.ksT.sub.ksG.sub.dsT.sub.ds.sup.mC.sub.dsA- .sub.dsG.sub.ds.sup.mC.sub.dsG.sub.dsA.sub.dsG.sub.dsT.sub.ds.sup.mC.sub.k- sG.sub.ksG.sub.k 12.4 2700 2715 427 486169 G.sub.ks.sup.mC.sub.ks.sup.mC.sub.ksT.sub.dsG.sub.dsT.sub.ds.sup.mC- .sub.dsA.sub.dsG.sub.ds.sup.mC.sub.dsG.sub.dsA.sub.dsG.sub.dsT.sub.ks.sup.- mC.sub.ksG.sub.k 24.5 2701 2716 428 486170 A.sub.ksG.sub.ks.sup.mC.sub.ks.sup.mC.sub.dsT.sub.dsG.sub.dsT.sub.d- s.sup.mC.sub.dsA.sub.dsG.sub.ds.sup.mC.sub.dsG.sub.dsA.sub.dsG.sub.ksT.sub- .ks.sup.mC.sub.k 16.3 2702 2717 429 486171 .sup.mC.sub.ksA.sub.ksG.sub.ksT.sub.dsG.sub.ds.sup.mC.sub.dsA.sub.d- sT.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsA.sub.dsA.sub.dsA.sub.dsA.sub.ks.sup.- mC.sub.ksG.sub.k 31.8 2744 2759 490 486172 T.sub.ks.sup.mC.sub.ksA.sub.ksG.sub.dsT.sub.dsG.sub.ds.sup.mC.sub.d- sA.sub.dsT.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsA.sub.dsA.sub.dsA.sub.ksA.sub- .ks.sup.mC.sub.k 23.1 2745 2760 491 486173 .sup.mC.sub.ksT.sub.ks.sup.mC.sub.ksA.sub.dsG.sub.dsT.sub.dsG.sub.d- s.sup.mC.sub.dsA.sub.dsT.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsA.sub.dsA.sub.k- sA.sub.ksA.sub.k 23 2746 2761 492 486174 T.sub.ks.sup.mC.sub.ksT.sub.ks.sup.mC.sub.dsA.sub.dsG.sub.dsT.sub.d- sG.sub.ds.sup.mC.sub.dsA.sub.dsT.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsA.sub.k- sA.sub.ksA.sub.k 50.9 2747 2762 493 486175 G.sub.ksT.sub.ks.sup.mC.sub.ksT.sub.ds.sup.mC.sub.dsA.sub.dsG.sub.d- sT.sub.dsG.sub.ds.sup.mC.sub.dsA.sub.dsT.sub.ds.sup.mC.sub.ds.sup.mC.sub.k- sA.sub.ksA.sub.k 17.2 2748 2763 494 486176 G.sub.ksG.sub.ksG.sub.ksT.sub.ds.sup.mC.sub.dsT.sub.ds.sup.mC.sub.d- sA.sub.dsG.sub.dsT.sub.dsG.sub.ds.sup.mC.sub.dsA.sub.dsT.sub.ks.sup.mC.sub- .ks.sup.mC.sub.k 37.6 2750 2765 430 486177 .sup.mC.sub.ksA.sub.ksA.sub.ksT.sub.dsA.sub.dsA.sub.dsA.sub.dsT.sub- .dsA.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsG.sub.dsA.sub.dsG.sub.ksG.sub.ksA.s- ub.k 40 2772 2787 495 486178 A.sub.ks.sup.mC.sub.ksA.sub.ksA.sub.dsT.sub.dsA.sub.dsA.sub.dsA.sub- .dsT.sub.dsA.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsG.sub.dsA.sub.ksG.sub.ksG.s- ub.k 11.3 2773 2788 23 486179 A.sub.ksG.sub.ksA.sub.ks.sup.mC.sub.dsA.sub.dsA.sub.dsT.sub.dsA.sub- .dsA.sub.dsA.sub.dsT.sub.dsA.sub.ds.sup.mC.sub.ds.sup.mC.sub.ksG.sub.ksA.s- ub.k 13.5 2775 2790 496 486180 .sup.mC.sub.ksA.sub.ksGkA.sub.ds.sup.mC.sub.dsA.sub.dsA.sub.dsT.sub- .dsA.sub.dsA.sub.dsA.sub.dsT.sub.dsA.sub.ds.sup.mC.sub.ks.sup.mC.sub.ksG.s- ub.k 18.6 2776 2791 497

Example 9

ASOs Designed to Target a Human DMPK RNA Transcript

[0682] A series of antisense oligonucleotides (ASOs) were designed to target hDMPK. The newly designed ASOs were prepared using standard oligonucleotide synthesis well known in the art and are described in Table 13 to 18, below. Subscripts "s" indicate phosphorothioate internucleoside linkages; subscripts "k" indicate 6'-(S)--CH.sub.3 bicyclic nucleosides (cEt); subscripts "e" indicate 2'-O-methoxyethyl (MOE) modified nucleosides; and subscripts "d" indicate .beta.-D-2'-deoxyribonucleosides. ".sup.mC" indicates 5-methylcytosine nucleosides.

[0683] The antisense oligonucleotides targeted to a human DMPK nucleic acid were tested for their effect on DMPK RNA transcript in vitro. Cultured HepG2 cells at a density of 20,000 cells per well were transfected using electroporation with 4,500 nM antisense oligonucleotide. After approximately 24 hours, RNA was isolated from the cells and DMPK transcript levels were measured by quantitative real-time PCR. DMPK RNA transcript levels were adjusted according to total RNA content, as measured by RIBOGREEN.RTM.. Results are presented as percent expression of DMPK, relative to untreated control cells, with "% Target Expression" representing the percent expression of DMPK relative to untreated control cells

[0684] All the antisense oligonucleotides listed in Table 13 target SEQ ID NO: 1 (GENBANK Accession No. NM_001081560.1). All the antisense oligonucleotides listed in Table 14 to 18 target SEQ ID NO: 2 (the complement of GENBANK Accession No. NT_011109.15 truncated from nucleotide; 185406 to 18555106). `Target start site` indicates the 5'-most nucleoside to which the antisense oligonucleotide is targeted in the human genomic gene sequence. `Target stop site` indicates the 3'-most nucleoside to which the antisense oligonucleotide is targeted in the human genomic sequence.

TABLE-US-00013 TABLE 13 Inhibition of human DMPK RNA transcript in HepG2 cells targeting SEQ ID NO: 1 Start Site Stop Site ISIS % Target on Seq on Seq Seq ID No. Sequence Expression ID: 1 ID: 1 No. UTC N/A 100 N/A N/A 445569 .sup.mC.sub.esG.sub.esG.sub.esA.sub.esG.sub.es.sup.mC.sub.dsG.sub.d- sG.sub.dsT.sub.dsT.sub.dsG.sub.dsT.sub.dsG.sub.dsA.sub.dsA.sub.ds.sup.mC.s- ub.esT.sub.esG.sub.esG.sub.es.sup.mC.sub.e 36.7 2163 2182 24 486178 A.sub.ks.sup.mC.sub.ksA.sub.ksA.sub.dsT.sub.dsA.sub.dsA.sub.dsA.sub- .dsT.sub.dsA.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsG.sub.dsA.sub.ksG.sub.ksG.s- ub.k 21.3 2773 2788 23 569403 .sup.mC.sub.ksA.sub.ks.sup.mC.sub.ksG.sub.dsG.sub.dsA.sub.dsA.sub.d- sG.sub.ds.sup.mC.sub.dsA.sub.ds.sup.mC.sub.dsG.sub.dsA.sub.ds.sup.mC.sub.k- sA.sub.ks.sup.mC.sub.k 18.8 542 557 498 569404 T.sub.ks.sup.mC.sub.ksA.sub.ks.sup.mC.sub.dsG.sub.dsG.sub.dsA.sub.d- sA.sub.dsG.sub.ds.sup.mC.sub.dsA.sub.ds.sup.mC.sub.dsG.sub.dsA.sub.ks.sup.- mC.sub.ksA.sub.k 25.2 543 558 499 569405 .sup.mC.sub.ksT.sub.ks.sup.mC.sub.ksA.sub.ds.sup.mC.sub.dsG.sub.dsG- .sub.dsA.sub.dsA.sub.dsG.sub.ds.sup.mC.sub.dsA.sub.ds.sup.mC.sub.dsG.sub.k- sA.sub.ks.sup.mC.sub.k 21.2 544 559 500 569406 .sup.mC.sub.ks.sup.mC.sub.ksT.sub.ks.sup.mC.sub.dsT.sub.ds.sup.mC.s- ub.ds.sup.mC.sub.dsT.sub.ds.sup.mC.sub.dsA.sub.ds.sup.mC.sub.dsG.sub.dsG.s- ub.dsA.sub.ksA.sub.ksG.sub.k 27.9 550 565 343 569407 G.sub.ksT.sub.ks.sup.mC.sub.ks.sup.mC.sub.ds.sup.mC.sub.dsT.sub.ds.- sup.mC.sub.dsT.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsT.sub.ds.sup.mC.sub.dsA.s- ub.ds.sup.mC.sub.ksG.sub.ksG.sub.k 30.9 553 568 501 569408 .sup.mC.sub.ksG.sub.ksT.sub.ks.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.s- ub.dsT.sub.ds.sup.mC.sub.dsT.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsT.sub.ds.su- p.mC.sub.dsA.sub.ks.sup.mC.sub.ksG.sub.k 32.8 554 569 502 569409 .sup.mC.sub.ks.sup.mC.sub.ks.sup.mC.sub.ksA.sub.dsT.sub.dsT.sub.ds.- sup.mC.sub.dsA.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsA.sub.dsA.sub.ds.sup.mC.s- ub.dsA.sub.ks.sup.mC.sub.ksG.sub.k 33 568 583 503 569410 .sup.mC.sub.ks.sup.mC.sub.ks.sup.mC.sub.ks.sup.mC.sub.dsA.sub.dsT.s- ub.dsT.sub.ds.sup.mC.sub.dsA.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsA.sub.dsA.s- ub.ds.sup.mC.sub.ksA.sub.ks.sup.mC.sub.k 42.1 569 584 504 569411 T.sub.ks.sup.mC.sub.ks.sup.mC.sub.ks.sup.mC.sub.ds.sup.mC.sub.dsA.s- ub.dsT.sub.dsT.sub.ds.sup.mC.sub.dsA.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsA.s- ub.dsA.sub.ks.sup.mC.sub.ksA.sub.k 68.6 570 585 505 569412 G.sub.ksT.sub.ks.sup.mC.sub.ks.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.s- ub.dsA.sub.dsT.sub.dsT.sub.ds.sup.mC.sub.dsA.sub.ds.sup.mC.sub.ds.sup.mC.s- ub.dsA.sub.ksA.sub.ks.sup.mC.sub.k 60.7 571 586 506 569413 G.sub.ksG.sub.ksT.sub.ks.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.- sup.mC.sub.dsA.sub.dsT.sub.dsT.sub.ds.sup.mC.sub.dsA.sub.ds.sup.mC.sub.ds.- sup.mC.sub.ksA.sub.ksA.sub.k 65.1 572 587 507 569414 .sup.mC.sub.ksG.sub.ksG.sub.ksT.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.- sup.mC.sub.ds.sup.mC.sub.dsA.sub.dsT.sub.dsT.sub.ds.sup.mC.sub.dsA.sub.ds.- sup.mC.sub.ks.sup.mC.sub.ksA.sub.k 54.4 573 588 508 569415 .sup.mC.sub.ks.sup.mC.sub.ksG.sub.ksG.sub.dsT.sub.ds.sup.mC.sub.ds.- sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsA.sub.dsT.sub.dsT.sub.ds.sup.mC.s- ub.dsA.sub.ks.sup.mC.sub.ks.sup.mC.sub.k 51.3 574 589 509 569416 G.sub.ks.sup.mC.sub.ks.sup.mC.sub.ksG.sub.dsG.sub.dsT.sub.ds.sup.mC- .sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsA.sub.dsT.sub.dsT.sub.ds.- sup.mC.sub.ksA.sub.ks.sup.mC.sub.k 57.9 575 590 510 569417 .sup.mC.sub.ksG.sub.ks.sup.mC.sub.ks.sup.mC.sub.dsG.sub.dsG.sub.dsT- .sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsA.sub.dsT.s- ub.dsT.sub.ks.sup.mC.sub.ksA.sub.k 43.2 576 591 511 569418 .sup.mC.sub.ks.sup.mC.sub.ksG.sub.ks.sup.mC.sub.ds.sup.mC.sub.dsG.s- ub.dsG.sub.dsT.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub- .dsA.sub.dsT.sub.ksT.sub.ks.sup.mC.sub.k 79.3 577 592 512 569419 A.sub.ks.sup.mC.sub.ks.sup.mC.sub.ksG.sub.ds.sup.mC.sub.ds.sup.mC.s- ub.dsG.sub.dsG.sub.dsT.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.su- p.mC.sub.dsA.sub.ksT.sub.ksT.sub.k 36 578 593 513 569420 .sup.mC.sub.ksA.sub.ks.sup.mC.sub.ks.sup.mC.sub.dsG.sub.ds.sup.mC.s- ub.ds.sup.mC.sub.dsG.sub.dsG.sub.dsT.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.su- p.mC.sub.ds.sup.mC.sub.ksA.sub.ksT.sub.k 36.2 579 594 514 569421 .sup.mC.sub.ks.sup.mC.sub.ksA.sub.ks.sup.mC.sub.ds.sup.mC.sub.dsG.s- ub.ds.sup.mC.sub.ds.sup.mC.sub.dsG.sub.dsG.sub.dsT.sub.ds.sup.mC.sub.ds.su- p.mC.sub.ds.sup.mC.sub.ks.sup.mC.sub.ksA.sub.k 34.7 580 595 515 569422 T.sub.ks.sup.mC.sub.ks.sup.mC.sub.ksA.sub.ds.sup.mC.sub.ds.sup.mC.s- ub.dsG.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsG.sub.dsG.sub.dsT.sub.ds.sup.mC.s- ub.ds.sup.mC.sub.ks.sup.mC.sub.ks.sup.mC.sub.k 40 581 596 516 569423 A.sub.ksT.sub.ks.sup.mC.sub.ks.sup.mC.sub.dsA.sub.ds.sup.mC.sub.ds.- sup.mC.sub.dsG.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsG.sub.dsG.sub.dsT.sub.ds.- sup.mC.sub.ks.sup.mC.sub.ks.sup.mC.sub.k 31.6 582 597 517 569424 G.sub.ksA.sub.ksT.sub.ks.sup.mC.sub.ds.sup.mC.sub.dsA.sub.ds.sup.mC- .sub.ds.sup.mC.sub.dsG.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsG.sub.dsG.sub.dsT- .sub.ks.sup.mC.sub.ks.sup.mC.sub.k 56 583 598 518 569425 T.sub.ksG.sub.ksA.sub.ksT.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsA.sub.d- s.sup.mC.sub.ds.sup.mC.sub.dsG.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsG.sub.dsG- .sub.ksT.sub.ks.sup.mC.sub.k 53.9 584 599 519 569426 G.sub.ksT.sub.ksG.sub.ksA.sub.dsT.sub.ds.sup.mC.sub.ds.sup.mC.sub.d- sA.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsG.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsG- .sub.ksG.sub.ksT.sub.k 54.1 585 600 520 569427 .sup.mC.sub.ksG.sub.ksT.sub.ksG.sub.dsA.sub.dsT.sub.ds.sup.mC.sub.d- s.sup.mC.sub.dsA.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsG.sub.ds.sup.mC.sub.ds.- sup.mC.sub.ksG.sub.ksG.sub.k 34.8 586 601 521 569428 .sup.mC.sub.ksA.sub.ksT.sub.ks.sup.mC.sub.ds.sup.mC.sub.dsT.sub.dsG- .sub.dsG.sub.dsA.sub.dsA.sub.dsG.sub.dsG.sub.ds.sup.mC.sub.dsG.sub.ksA.sub- .ksA.sub.k 71 611 626 522 569429 T.sub.ks.sup.mC.sub.ksA.sub.ksT.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsT- .sub.dsG.sub.dsG.sub.dsA.sub.dsA.sub.dsGdaG.sub.ds.sup.mC.sub.ksG.sub.ksA.- sub.k 51.1 612 627 523 569430 A.sub.ksG.sub.ksT.sub.ksT.sub.ds.sup.mC.sub.dsT.sub.ds.sup.mC.sub.d- sA.sub.dsT.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsT.sub.dsG.sub.dsG.sub.ksA.sub- .ksA.sub.k 69.2 617 632 524 569431 T.sub.ksA.sub.ksG.sub.ksT.sub.dsT.sub.ds.sup.mC.sub.dsT.sub.ds.sup.- mC.sub.dsA.sub.dsT.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsT.sub.dsG.sub.ksG.sub- .ksA.sub.k 48.6 618 633 525 569432 G.sub.ksT.sub.ksA.sub.ksG.sub.dsT.sub.dsT.sub.ds.sup.mC.sub.dsT.sub- .ds.sup.mC.sub.dsA.sub.dsT.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsT.sub.ksG.sub- .ksG.sub.k 29.6 619 634 526 569433 .sup.mC.sub.ksA.sub.ksG.sub.ksG.sub.dsT.sub.dsA.sub.ds.sup.mC.sub.d- sA.sub.dsG.sub.dsG.sub.dsT.sub.dsA.sub.dsG.sub.dsT.sub.ksT.sub.ks.sup.mC.s- ub.k 36.5 628 643 527 569434 .sup.mC.sub.ks.sup.mC.sub.ksA.sub.ksG.sub.dsG.sub.dsT.sub.dsA.sub.d- s.sup.mC.sub.dsA.sub.dsG.sub.dsG.sub.dsT.sub.dsA.sub.dsG.sub.ksT.sub.ksT.s- ub.k 51 629 644 528 560435 G.sub.ksA.sub.ks.sup.mC.sub.ks.sup.mC.sub.dsA.sub.dsG.sub.dsG.sub.d- sT.sub.dsA.sub.ds.sup.mC.sub.dsA.sub.dsG.sub.dsG.sub.dsT.sub.ksA.sub.ksG.s- ub.k 49.9 631 646 529 569436 .sup.mC.sub.ksT.sub.ks.sup.mC.sub.ks.sup.mC.sub.dsA.sub.dsT.sub.dsG- .sub.dsA.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsA.sub.dsG.sub.dsG.sub.dsT.sub.k- sA.sub.ks.sup.mC.sub.k 41 637 652 530 569437 A.sub.ks.sup.mC.sub.ksT.sub.ks.sup.mC.sub.ds.sup.mC.sub.dsA.sub.dsT- .sub.dsG.sub.dsA.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsA.sub.dsG.sub.dsG.sub.k- sT.sub.ksA.sub.k 32.9 638 653 531 569438 T.sub.ksA.sub.ks.sup.mC.sub.ksT.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsA- .sub.dsT.sub.dsG.sub.dsA.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsA.sub.dsG.sub.k- sG.sub.ksT.sub.k 25.7 639 654 532 569439 A.sub.ksT.sub.ksA.sub.ks.sup.mC.sub.dsT.sub.ds.sup.mC.sub.ds.sup.mC- .sub.dsA.sub.dsT.sub.dsG.sub.dsA.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsA.sub.k- sG.sub.ksG.sub.k 9.4 640 655 533 569440 A.sub.ksA.sub.ksT.sub.ksA.sub.ds.sup.mC.sub.dsT.sub.ds.sup.mC.sub.d- s.sup.mC.sub.dsA.sub.dsT.sub.dsG.sub.dsA.sub.ds.sup.mC.sub.ds.sup.mC.sub.k- sA.sub.ksG.sub.k 21.2 641 656 534 569441 T.sub.ksA.sub.ksA.sub.ksT.sub.dsA.sub.ds.sup.mC.sub.dsT.sub.ds.sup.- mC.sub.ds.sup.mC.sub.dsA.sub.dsT.sub.dsG.sub.dsA.sub.ds.sup.mC.sub.ks.sup.- mC.sub.ksA.sub.k 30.8 642 657 535 569442 G.sub.ksT.sub.ksA.sub.ksA.sub.dsT.sub.dsA.sub.ds.sup.mC.sub.dsT.sub- .ds.sup.mC.sub.ds.sup.mC.sub.dsA.sub.dsT.sub.dsG.sub.dsA.sub.ks.sup.mC.sub- .ks.sup.mC.sub.k 29.8 643 658 536 569443 .sup.mC.sub.ksG.sub.ksT.sub.ksA.sub.dsA.sub.dsT.sub.dsA.sub.ds.sup.- mC.sub.dsT.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsA.sub.dsT.sub.dsG.sub.ksA.sub- .ks.sup.mC.sub.k 25.3 644 659 537 569444 .sup.mC.sub.ksT.sub.ksT.sub.ksG.sub.ds.sup.mC.sub.dsT.sub.ds.sup.mC- .sub.dsA.sub.dsG.sub.ds.sup.mC.sub.dsA.sub.dsG.sub.dsT.sub.dsG.sub.ksT.sub- .ks.sup.mC.sub.k 19.3 676 691 538 569445 A.sub.ks.sup.mC.sub.ksT.sub.ksT.sub.dsG.sub.ds.sup.mC.sub.dsT.sub.d- s.sup.mC.sub.dsA.sub.dsG.sub.ds.sup.mC.sub.dsA.sub.dsG.sub.dsT.sub.ksG.sub- .ksT.sub.k 35 677 692 539 569446 A.sub.ksA.sub.ks.sup.mC.sub.ksT.sub.dsT.sub.dsG.sub.ds.sup.mC.sub.d- sT.sub.ds.sup.mC.sub.dsA.sub.dsG.sub.ds.sup.mC.sub.dsA.sub.dsG.sub.ksT.sub- .ksG.sub.k 30 678 693 540 569447 A.sub.ksA.sub.ksA.sub.ks.sup.mC.sub.dsT.sub.dsT.sub.dsG.sub.ds.sup.- mC.sub.dsT.sub.ds.sup.mC.sub.dsA.sub.dsG.sub.ds.sup.mC.sub.dsA.sub.ksG.sub- .ksT.sub.k 32.2 679 694 344 569448 .sup.mC.sub.ks.sup.mC.sub.ksA.sub.ksA.sub.dsA.sub.ds.sup.mC.sub.dsT- .sub.dsT.sub.dsG.sub.ds.sup.mC.sub.dsT.sub.ds.sup.mC.sub.dsA.sub.dsG.sub.k- s.sup.mC.sub.ksA.sub.k 30.1 681 696 346 569449 .sup.mC.sub.ks.sup.mC.sub.ks.sup.mC.sub.ksA.sub.dsA.sub.dsA.sub.ds.- sup.mC.sub.dsT.sub.dsT.sub.dsG.sub.ds.sup.mC.sub.dsT.sub.ds.sup.mC.sub.dsA- .sub.ksG.sub.ks.sup.mC.sub.k 18.4 682 697 347 569450 .sup.mC.sub.ks.sup.mC.sub.ks.sup.mC.sub.ks.sup.mC.sub.dsA.sub.dsA.s- ub.dsA.sub.ds.sup.mC.sub.dsT.sub.dsT.sub.dsG.sub.ds.sup.mC.sub.dsT.sub.ds.- sup.mC.sub.ksA.sub.ksG.sub.k 44.8 683 698 348 569451 G.sub.ks.sup.mC.sub.ksT.sub.ks.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.s- ub.ds.sup.mC.sub.dsA.sub.dsA.sub.dsA.sub.ds.sup.mC.sub.dsT.sub.dsT.sub.dsG- .sub.ks.sup.mC.sub.ksT.sub.k 47 686 701 541 569452 .sup.mC.sub.ksG.sub.ks.sup.mC.sub.ksT.sub.ds.sup.mC.sub.ds.sup.mC.s- ub.ds.sup.mC.sub.ds.sup.mC.sub.dsA.sub.dsA.sub.dsA.sub.ds.sup.mC.sub.dsT.s- ub.dsT.sub.ksG.sub.ks.sup.mC.sub.k 35.4 687 702 542 569453 .sup.mC.sub.ks.sup.mC.sub.ksG.sub.ks.sup.mC.sub.dsT.sub.ds.sup.mC.s- ub.ds.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsA.sub.dsA.sub.dsA.sub.ds.su- p.mC.sub.dsT.sub.ksT.sub.ksG.sub.k 46.6 688 703 543 569454 T.sub.ks.sup.mC.sub.ks.sup.mC.sub.ksG.sub.ds.sup.mC.sub.dsT.sub.ds.- sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsA.sub.dsA.sub.dsA.s- ub.ds.sup.mC.sub.ksT.sub.ksT.sub.k 29.4 689 704 544 569455 A.sub.ksT.sub.ks.sup.mC.sub.ks.sup.mC.sub.dsG.sub.ds.sup.mC.sub.dsT- .sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsA.sub.dsA.s- ub.dsA.sub.ks.sup.mC.sub.ksT.sub.k 36.9 690 705 545 569456 A.sub.ksA.sub.ksT.sub.ks.sup.mC.sub.ds.sup.mC.sub.dsG.sub.ds.sup.mC- .sub.dsT.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsA.s- ub.dsA.sub.ksA.sub.ks.sup.mC.sub.k 32.9 691 706 546 569457 G.sub.ksA.sub.ksA.sub.ksT.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsG.sub.d- s.sup.mC.sub.dsT.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.s- ub.dsA.sub.ksA.sub.ksA.sub.k 41.7 692 707 547 569458 G.sub.ksG.sub.ksA.sub.ksA.sub.dsT.sub.ds.sup.mC.sub.ds.sup.mC.sub.d- sG.sub.ds.sup.mC.sub.dsT.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.- sup.mC.sub.ksA.sub.ksA.sub.k 36.4 693 708 548 569459 .sup.mC.sub.ksG.sub.ksG.sub.ksA.sub.dsA.sub.dsT.sub.ds.sup.mC.sub.d- s.sup.mC.sub.dsG.sub.ds.sup.mC.sub.dsT.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.- sup.mC.sub.ks.sup.mC.sub.ksA.sub.k 30 694 709 549 569460 .sup.mC.sub.ks.sup.mC.sub.ksG.sub.ksG.sub.dsA.sub.dsA.sub.dsT.sub.d- s.sup.mC.sub.ds.sup.mC.sub.dsG.sub.ds.sup.mC.sub.dsT.sub.ds.sup.mC.sub.ds.- sup.mC.sub.ks.sup.mC.sub.ks.sup.mC.sub.k 26.5 695 710 550

569461 G.sub.ks.sup.mC.sub.ks.sup.mC.sub.ksG.sub.dsG.sub.dsA.sub.dsA.sub.d- sT.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsG.sub.ds.sup.mC.sub.dsT.sub.ds.sup.mC- .sub.ks.sup.mC.sub.ks.sup.mC.sub.k 36.5 696 711 551 569462 A.sub.ksG.sub.ksA.sub.ksA.sub.dsG.sub.ds.sup.mC.sub.dsGdg.sup.mC.su- b.dsG.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsA.sub.dsT.sub.ds.sup.mC.sub.ksT.su- b.ks.sup.mC.sub.k 26 713 728 552 569463 T.sub.ksA.sub.ksG.sub.ksA.sub.dsA.sub.dsG.sub.ds.sup.mC.sub.dsG.sub- .ds.sup.mC.sub.dsG.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsA.sub.dsT.sub.ks.sup.- mC.sub.ksT.sub.k 40.3 714 729 553 569464 G.sub.ksT.sub.ksA.sub.ksG.sub.dsA.sub.dsA.sub.dsG.sub.ds.sup.mC.sub- .dsG.sub.ds.sup.mC.sub.dsG.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsA.sub.ksT.sub- .ks.sup.mC.sub.k 28.9 715 730 554 569465 G.sub.ksG.sub.ksT.sub.ksA.sub.dsG.sub.dsA.sub.dsA.sub.dsG.sub.ds.su- p.mC.sub.dsG.sub.ds.sup.mC.sub.dsG.sub.ds.sup.mC.sub.ds.sup.mC.sub.ksA.sub- .ksT.sub.k 35.7 716 731 555 569466 A.sub.ksG.sub.ksG.sub.ksT.sub.dsA.sub.dsG.sub.dsA.sub.dsA.sub.dsG.s- ub.ds.sup.mC.sub.dsG.sub.ds.sup.mC.sub.dsG.sub.ds.sup.mC.sub.ks.sup.mC.sub- .ksA.sub.k 31.1 717 732 556 569467 .sup.mC.sub.ksA.sub.ksG.sub.ksG.sub.dsT.sub.dsA.sub.dsG.sub.dsAdACk- G.sub.ds.sup.mC.sub.dsG.sub.ds.sup.mC.sub.dsG.sub.ks.sup.mC.sub.ks.sup.mC.- sub.k 14.8 718 733 557 569468 .sup.mC.sub.ks.sup.mC.sub.ksA.sub.ksG.sub.dsG.sub.dsT.sub.dsA.sub.d- sG.sub.dsA.sub.dsA.sub.dsG.sub.ds.sup.mC.sub.dsG.sub.ds.sup.mC.sub.ksG.sub- .ks.sup.mC.sub.k 32.1 719 734 558 569469 G.sub.ks.sup.mC.sub.ks.sup.mCkA.sub.dsG.sub.dsG.sub.dsT.sub.dsA.sub- .dsG.sub.dsA.sub.dsA.sub.dsG.sub.ds.sup.mC.sub.dsG.sub.ks.sup.mC.sub.ksG.s- ub.k 54.5 720 735 559 569470 .sup.mC.sub.ksG.sub.ks.sup.mC.sub.ks.sup.mC.sub.dsA.sub.dsG.sub.dsG- .sub.dsT.sub.dsA.sub.dsG.sub.dsA.sub.dsA.sub.dsG.sub.ds.sup.mC.sub.ksG.sub- .ks.sup.mC.sub.k 50.5 721 736 560 569471 .sup.mC.sub.ks.sup.mC.sub.ksG.sub.ks.sup.mC.sub.ds.sup.mC.sub.dsA.s- ub.dsG.sub.dsG.sub.dsT.sub.dsA.sub.dsG.sub.dsA.sub.dsA.sub.dsG.sub.ks.sup.- mC.sub.ksG.sub.k 56.6 722 737 561 569472 T.sub.ks.sup.mC.sub.ks.sup.mC.sub.ksG.sub.ds.sup.mC.sub.ds.sup.mC.s- ub.dsA.sub.dsG.sub.dsG.sub.dsT.sub.dsA.sub.dsG.sub.dsA.sub.dsA.sub.ksG.sub- .ks.sup.mC.sub.k 44.1 723 738 562 569473 G.sub.ksA.sub.ks.sup.mC.sub.ksA.sub.dsA.sub.dsT.sub.ds.sup.mC.sub.d- sT.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsG.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsA- .sub.ksG.sub.ksG.sub.k 14.2 730 745 29 569474 T.sub.ksG.sub.ksA.sub.ks.sup.mC.sub.dsA.sub.dsA.sub.dsT.sub.ds.sup.- mC.sub.dsT.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsG.sub.ds.sup.mC.sub.ds.sup.mC- .sub.ksA.sub.ksG.sub.k 25.9 731 746 563 569475 A.sub.ksT.sub.ksG.sub.ksA.sub.ds.sup.mC.sub.dsA.sub.dsA.sub.dsT.sub- .ds.sup.mC.sub.dsT.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsG.sub.ds.sup.mC.sub.k- s.sup.mC.sub.ksA.sub.k 28.7 732 747 564 569476 .sup.mC.sub.ksA.sub.ksT.sub.ksG.sub.dsA.sub.ds.sup.mC.sub.dsA.sub.d- sA.sub.dsT.sub.ds.sup.mC.sub.dsT.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsG.sub.k- s.sup.mC.sub.ks.sup.mC.sub.k 27.4 733 748 565 569477 .sup.mC.sub.ks.sup.mC.sub.ksA.sub.ksT.sub.dsG.sub.dsA.sub.ds.sup.mC- .sub.dsA.sub.dsA.sub.dsT.sub.ds.sup.mC.sub.dsT.sub.ds.sup.mC.sub.ds.sup.mC- .sub.ksG.sub.ks.sup.mC.sub.k 52.4 734 749 566 569478 G.sub.ks.sup.mC.sub.ks.sup.mC.sub.ksA.sub.dsT.sub.dsG.sub.dsA.sub.d- s.sup.mC.sub.dsA.sub.dsA.sub.dsT.sub.ds.sup.mC.sub.dsT.sub.ds.sup.mC.sub.k- s.sup.mC.sub.ksG.sub.k 50.5 735 750 567 569479 G.sub.ksG.sub.ks.sup.mC.sub.ks.sup.mC.sub.dsA.sub.dsT.sub.dsG.sub.d- sA.sub.ds.sup.mC.sub.dsA.sub.dsA.sub.dsT.sub.ds.sup.mC.sub.dsT.sub.ks.sup.- mC.sub.ks.sup.mC.sub.k 48.4 736 751 568

TABLE-US-00014 TABLE 14 Inhibition of human DMPK RNA transcript in HepG2 cells targeting SEQ ID NO: 2 Start Site Stop Site ISIS % Target on Seq on Seq Seq ID No. Sequence Expression ID: 2 ID: 2 No. UTC N/A 100 N/A N/A 445569 .sup.mC.sub.esG.sub.esG.sub.esA.sub.esG.sub.es.sup.mC.sub.dsG.sub.d- sG.sub.dsT.sub.dsT.sub.dsG.sub.dsT.sub.dsG.sub.dsA.sub.dsA.sub.ds.sup.mC.s- ub.esT.sub.esG.sub.esG.sub.es.sup.mC.sub.e 31.4 13226 13245 24 486178 A.sub.ks.sup.mC.sub.ksA.sub.ksA.sub.dsT.sub.dsA.sub.dsA.sub.dsA.sub- .dsT.sub.dsA.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsG.sub.dsA.sub.ksG.sub.ksG.s- ub.k 25.3 13836 13851 23 570801 .sup.mC.sub.ks.sup.mC.sub.ksA.sub.ksA.sub.ds.sup.mC.sub.dsT.sub.dsG- .sub.dsT.sub.dsT.sub.ds.sup.mC.sub.dsT.sub.ds.sup.mC.sub.dsT.sub.dsT.sub.k- sA.sub.ksG.sub.k 22.7 10165 10180 569 570802 A.sub.ksA.sub.ks.sup.mC.sub.ks.sup.mC.sub.dsA.sub.dsA.sub.ds.sup.mC- .sub.dsT.sub.dsG.sub.dsT.sub.dsT.sub.ds.sup.mC.sub.dsT.sub.ds.sup.mC.sub.k- sT.sub.ksT.sub.k 22.6 10167 10182 570 570803 .sup.mC.sub.ks.sup.mC.sub.ksA.sub.ksG.sub.dsT.sub.dsA.sub.dsA.sub.d- sT.sub.dsA.sub.dsA.sub.dsA.sub.dsA.sub.dsG.sub.ds.sup.mC.sub.ksT.sub.ksG.s- ub.k 37.4 10190 10205 571 570804 G.sub.ksT.sub.ks.sup.mC.sub.ks.sup.mC.sub.dsA.sub.dsG.sub.dsT.sub.d- sA.sub.dsA.sub.dsT.sub.dsA.sub.dsA.sub.dsA.sub.dsA.sub.ksG.sub.ks.sup.mC.s- ub.k 24.9 10192 10207 572 570805 G.sub.ksT.sub.ksT.sub.ksG.sub.dsT.sub.ds.sup.mC.sub.ds.sup.mC.sub.d- sA.sub.dsG.sub.dsT.sub.dsA.sub.dsA.sub.dsT.sub.dsA.sub.ksA.sub.ksA.sub.k 23.8 10195 10210 573 570806 A.sub.ksT.sub.ksG.sub.ksT.sub.dsT.sub.dsG.sub.dsT.sub.ds.sup.mC.sub- .ds.sup.mC.sub.dsA.sub.dsG.sub.dsT.sub.dsA.sub.dsA.sub.ksT.sub.ksA.sub.k 21.9 10197 10212 574 570807 TkA.sub.ksA.sub.ksT.sub.dsG.sub.dsT.sub.dsT.sub.dsG.sub.dsT.sub.ds.- sup.mC.sub.ds.sup.mC.sub.dsA.sub.dsG.sub.dsT.sub.ksA.sub.ksA.sub.k 20 10199 10214 575 570808 T.sub.ksG.sub.ksT.sub.ksA.sub.dsA.sub.dsT.sub.dsG.sub.dsT.sub.dsT.s- ub.dsG.sub.dsT.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsA.sub.ksG.sub.ksT.sub.k 11.5 10201 10216 31 570809 T.sub.ksT.sub.ks.sup.mC.sub.ksA.sub.dsA.sub.dsT.sub.ds.sup.mC.sub.d- s.sup.mC.sub.dsT.sub.dsG.sub.dsA.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC- .sub.ksA.sub.ks.sup.mC.sub.k 34.7 10279 10294 576 570810 G.sub.ksG.sub.ksT.sub.ksT.sub.ds.sup.mC.sub.dsA.sub.dsA.sub.dsT.sub- .ds.sup.mC.sub.ds.sup.mC.sub.dsT.sub.dsG.sub.dsA.sub.ds.sup.mC.sub.ks.sup.- mC.sub.ks.sup.mC.sub.k 76.4 10281 10296 577 570811 T.sub.ksG.sub.ksG.sub.ksG.sub.dsT.sub.dsT.sub.ds.sup.mC.sub.dsA.sub- .dsA.sub.dsT.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsT.sub.dsGkA.sub.ks.sup.mC.s- ub.k 72.4 10283 10298 578 570812 G.sub.ksA.sub.ksT.sub.ksG.sub.dsG.sub.dsG.sub.dsT.sub.dsT.sub.ds.su- p.mC.sub.dsA.sub.dsA.sub.dsT.sub.ds.sup.mC.sub.ds.sup.mC.sub.ksT.sub.ksG.s- ub.k 49 10285 10300 579 570813 A.sub.ksG.sub.ksG.sub.ksA.sub.dsT.sub.dsG.sub.dsG.sub.dsG.sub.dsT.s- ub.dsT.sub.ds.sup.mC.sub.dsA.sub.dsA.sub.dsT.sub.ks.sup.mC.sub.ks.sup.mC.s- ub.k 80.8 10287 10302 580 570814 A.sub.ksG.sub.ksA.sub.ksG.sub.dsG.sub.dsA.sub.dsT.sub.dsG.sub.dsG.s- ub.dsG.sub.dsT.sub.dsT.sub.ds.sup.mC.sub.dsA.sub.ksA.sub.ksT.sub.k 43.3 10289 10304 581 570815 A.sub.ksT.sub.ksA.sub.ksG.sub.dsA.sub.dsG.sub.dsG.sub.dsA.sub.dsT.s- ub.dsG.sub.dsG.sub.dsG.sub.dsT.sub.dsT.sub.ks.sup.mC.sub.ksA.sub.k 63.2 10291 10306 582 570816 .sup.mC.sub.ks.sup.mC.sub.ks.sup.mC.sub.ksT.sub.ds.sup.mC.sub.ds.su- p.mC.sub.dsT.sub.dsG.sub.dsT.sub.dsG.sub.dsG.sub.dsG.sub.dsA.sub.dsA.sub.k- s.sup.mC.sub.ksA.sub.k 38.8 10349 10364 583 570817 G.sub.ksT.sub.ks.sup.mC.sub.ks.sup.mC.sub.ds.sup.mC.sub.dsT.sub.ds.- sup.mC.sub.ds.sup.mC.sub.dsT.sub.dsG.sub.dsT.sub.dsG.sub.dsG.sub.dsG.sub.k- sA.sub.ksA.sub.k 91 10351 10366 584 570818 .sup.mC.sub.ksA.sub.ksG.sub.ksT.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.- sup.mC.sub.dsT.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsT.sub.dsG.sub.dsT.sub.dsG- .sub.ksG.sub.ksG.sub.k 64.8 10353 10368 585 570819 A.sub.ksG.sub.ks.sup.mC.sub.ksA.sub.dsG.sub.dsT.sub.ds.sup.mC.sub.d- s.sup.mC.sub.ds.sup.mC.sub.dsT.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsT.sub.dsG- .sub.ksT.sub.ksG.sub.k 28.5 10355 10370 586 570820 A.sub.ks.sup.mC.sub.ksT.sub.ks.sup.mC.sub.dsA.sub.dsG.sub.ds.sup.mC- .sub.dsT.sub.dsG.sub.dsT.sub.dsG.sub.dsG.sub.dsG.sub.dsA.sub.ksA.sub.ksG.s- ub.k 62.9 10417 10432 587 570821 .sup.mC.sub.ks.sup.mC.sub.ks.sup.mC.sub.ksA.sub.ds.sup.mC.sub.dsT.s- ub.ds.sup.mC.sub.dsA.sub.dsG.sub.ds.sup.mC.sub.dsT.sub.dsG.sub.dsT.sub.dsG- .sub.ksG.sub.ksG.sub.k 79.9 10420 10435 588 570822 A.sub.ks.sup.mC.sub.ks.sup.mC.sub.ks.sup.mC.sub.ds.sup.mC.sub.dsA.s- ub.ds.sup.mC.sub.dsT.sub.ds.sup.mC.sub.dsA.sub.dsG.sub.ds.sup.mC.sub.dsT.s- ub.dsG.sub.ksT.sub.ksG.sub.k 47.5 10422 10437 589 570823 A.sub.ks.sup.mC.sub.ksA.sub.ks.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.s- ub.ds.sup.mCdA.sub.ds.sup.mC.sub.dsT.sub.ds.sup.mC.sub.dsA.sub.dsG.sub.ds.- sup.mC.sub.ksT.sub.ksG.sub.k 78.1 10424 10439 590 570824 G.sub.ks.sup.mC.sub.ksA.sub.ks.sup.mC.sub.dsA.sub.ds.sup.mC.sub.ds.- sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsA.sub.ds.sup.mC.sub.dsT.sub.ds.su- p.mC.sub.dsA.sub.ksG.sub.ks.sup.mC.sub.k 82.5 10426 10441 591 570825 T.sub.ks.sup.mC.sub.ksA.sub.ksG.sub.ds.sup.mC.sub.dsA.sub.ds.sup.mC- .sub.dsA.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsA.s- ub.ds.sup.mC.sub.ksT.sub.ks.sup.mC.sub.k 52.6 10429 10444 592 570826 G.sub.ksT.sub.ksG.sub.ksG.sub.dsT.sub.ds.sup.mC.sub.ds.sup.mC.sub.d- sT.sub.dsA.sub.dsA.sub.dsG.sub.dsA.sub.ds.sup.mC.sub.dsT.sub.ksG.sub.ksG.s- ub.k 30.9 10474 10489 593 570827 G.sub.ksA.sub.ksT.sub.ksG.sub.dsT.sub.dsG.sub.dsG.sub.dsT.sub.ds.su- p.mC.sub.ds.sup.mC.sub.dsT.sub.dsA.sub.dsA.sub.dsG.sub.ksA.sub.ks.sup.mC.s- ub.k 25.5 10477 10492 594 570828 .sup.mC.sub.ksA.sub.ksG.sub.ksA.sub.dsT.sub.dsG.sub.dsT.sub.dsG.sub- .dsG.sub.dsT.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsT.sub.dsA.sub.ksA.sub.ksG.s- ub.k 18.6 10479 10494 595 570829 .sup.mC.sub.ks.sup.mC.sub.ksT.sub.ks.sup.mC.sub.ds.sup.mC.sub.dsA.s- ub.ds.sup.mC.sub.dsA.sub.dsG.sub.dsA.sub.dsT.sub.dsG.sub.dsT.sub.dsG.sub.k- sG.sub.ksT.sub.k 44.5 10485 10500 596 570830 .sup.mC.sub.ksA.sub.ks.sup.mC.sub.ks.sup.mC.sub.dsT.sub.ds.sup.mC.s- ub.ds.sup.mC.sub.dsA.sub.ds.sup.mC.sub.dsA.sub.dsG.sub.dsA.sub.dsT.sub.dsG- .sub.ksT.sub.ksG.sub.k 67.4 10487 10502 597 570831 G.sub.ksG.sub.ks.sup.mC.sub.ks.sup.mC.sub.dsA.sub.ds.sup.mC.sub.ds.- sup.mC.sub.dsT.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsA.sub.ds.sup.mC.sub.dsA.s- ub.dsG.sub.ksA.sub.ksT.sub.k 56.3 10490 10505 598 570832 T.sub.ksG.sub.ks.sup.mC.sub.ksT.sub.dsT.sub.dsG.sub.dsG.sub.ds.sup.- mC.sub.dsT.sub.ds.sup.mC.sub.dsT.sub.dsG.sub.dsG.sub.ds.sup.mC.sub.ks.sup.- mC.sub.ksA.sub.k 42.4 10501 10516 599 570833 A.sub.ks.sup.mC.sub.ksT.sub.ksG.sub.ds.sup.mC.sub.dsT.sub.dsT.sub.d- sG.sub.dsG.sub.ds.sup.mC.sub.dsT.sub.ds.sup.mC.sub.dsT.sub.dsGtsG.sub.ks.s- up.mC.sub.k 16 10503 10518 600 570834 A.sub.ksG.sub.ksA.sub.ks.sup.mC.sub.dsT.sub.dsG.sub.ds.sup.mC.sub.d- sT.sub.dsT.sub.dsG.sub.dsG.sub.ds.sup.mC.sub.dsT.sub.ds.sup.mC.sub.ksT.sub- .ksG.sub.k 47.5 10505 10520 601 570835 G.sub.ksG.sub.ksA.sub.ksG.sub.dsA.sub.ds.sup.mC.sub.dsT.sub.dsG.sub- .ds.sup.mC.sub.dsT.sub.dsT.sub.dsG.sub.dsG.sub.ds.sup.mC.sub.ksT.sub.ks.su- p.mC.sub.k 37.2 10507 10522 602 570836 T.sub.ksG.sub.ks.sup.mC.sub.ksA.sub.dsG.sub.dsA.sub.ds.sup.mC.sub.d- s.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsT.sub.ds.sup.mC.sub.dsT.sub.dsT- .sub.ksMC.sub.ksT.sub.k 63.1 10556 10571 603 570837 .sup.mC.sub.ksT.sub.ks.sup.mC.sub.ks.sup.mC.sub.dsT.sub.ds.sup.mC.s- ub.ds.sup.mC.sub.ds.sup.mC.sub.dsT.sub.dsT.sub.dsG.sub.dsA.sub.ds.sup.mC.s- ub.dsA.sub.ksT.sub.ksG.sub.k 60.7 10579 10594 604 570838 .sup.mC.sub.ks.sup.mC.sub.ksA.sub.ksG.sub.dsA.sub.ds.sup.mC.sub.ds.- sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsA.sub.dsT.sub.dsG.s- ub.dsT.sub.ksT.sub.ks.sup.mC.sub.k 42.9 10609 10624 605 570839 G.sub.ksT.sub.ks.sup.mC.sub.ks.sup.mC.sub.dsA.sub.dsG.sub.dsA.sub.d- s.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsA.s- ub.dsT.sub.ksG.sub.ksT.sub.k 64.3 10611 10626 606 570840 G.sub.ksG.sub.ksG.sub.ksT.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsA.sub.d- sG.sub.dsA.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.- sup.mC.sub.ksA.sub.ksT.sub.k 68.5 10613 10628 607 570841 A.sub.ks.sup.mC.sub.ks.sup.mC.sub.ksT.sub.dsT.sub.ds.sup.mC.sub.dsT- .sub.dsG.sub.ds.sup.mC.sub.dsA.sub.dsG.sub.dsG.sub.dsG.sub.dsA.sub.ks.sup.- mC.sub.ksT.sub.k 14.9 10631 10646 608 570842 T.sub.ksA.sub.ksA.sub.ksA.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsT.sub.d- sT.sub.ds.sup.mC.sub.dsT.sub.dsG.sub.ds.sup.mC.sub.dsA.sub.dsG.sub.ksG.sub- .ksG.sub.k 51.7 10634 10649 609 570843 G.sub.ksA.sub.ksA.sub.ksA.sub.dsA.sub.dsG.sub.ds.sup.mC.sub.ds.sup.- mC.sub.ds.sup.mC.sub.dsT.sub.dsG.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC- .sub.ks.sup.mC.sub.ksT.sub.k 46.3 10684 10699 610 570844 T.sub.ksA.sub.ksG.sub.ksG.sub.dsA.sub.dsA.sub.dsA.sub.dsA.sub.dsG.s- ub.ds.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsT.sub.dsG.sub.ks.sup.mC.sub- .ks.sup.mC.sub.k 52.3 10687 10702 611 570845 .sup.mC.sub.ksT.sub.ksT.sub.ksA.sub.dsG.sub.dsG.sub.dsA.sub.dsAdA.s- ub.dsA.sub.dsG.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.ksT.sub.ksG.s- ub.k 53.8 10689 10704 612 570846 T.sub.ksG.sub.ks.sup.mC.sub.ksT.sub.dsT.sub.dsA.sub.dsG.sub.dsG.sub- .dsA.sub.dsA.sub.dsA.sub.dsA.sub.dsG.sub.ds.sup.mC.sub.ks.sup.mC.sub.ks.su- p.mC.sub.k 47.8 10691 10706 613 570847 T.sub.ks.sup.mC.sub.ksT.sub.ksG.sub.ds.sup.mC.sub.dsT.sub.dsT.sub.d- sA.sub.dsG.sub.dsG.sub.dsA.sub.dsA.sub.dsA.sub.dsA.sub.ksG.sub.ks.sup.mC.s- ub.k 43.9 10693 10708 614 570848 .sup.mC.sub.ksT.sub.ks.sup.mC.sub.ks.sup.mC.sub.dsT.sub.ds.sup.mC.s- ub.dsT.sub.dsG.sub.ds.sup.mC.sub.dsT.sub.dsT.sub.dsA.sub.dsG.sub.dsG.sub.k- sA.sub.ksA.sub.k 67.9 10697 10712 615 570849 .sup.mC.sub.ks.sup.mC.sub.ks.sup.mC.sub.ksT.sub.ds.sup.mC.sub.ds.su- p.mC.sub.dsT.sub.ds.sup.mC.sub.dsT.sub.dsG.sub.ds.sup.mC.sub.dsT.sub.dsT.s- ub.dsA.sub.ksG.sub.ksG.sub.k 50.8 10699 10714 616 570850 .sup.mC.sub.ksT.sub.ksG.sub.ksA.sub.dsT.sub.dsT.sub.dsT.sub.dsG.sub- .dsA.sub.dsG.sub.dsG.sub.dsA.sub.dsA.sub.dsG.sub.ksG.sub.ksG.sub.k 41.1 10759 10774 617 570851 T.sub.ks.sup.mC.sub.ks.sup.mC.sub.ksT.sub.dsG.sub.dsA.sub.dsT.sub.d- sT.sub.dsT.sub.dsG.sub.dsA.sub.dsG.sub.dsG.sub.dsA.sub.ksA.sub.ksG.sub.k 87.4 10761 10776 618 570852 .sup.mC.sub.ks.sup.mC.sub.ksT.sub.ks.sup.mC.sub.ds.sup.mC.sub.dsT.s- ub.dsG.sub.dsA.sub.dsT.sub.dsT.sub.dsT.sub.dsG.sub.dsA.sub.dsG.sub.ksG.sub- .ksA.sub.k 75.8 10763 10778 619 570853 G.sub.ksA.sub.ks.sup.mC.sub.ks.sup.mC.sub.dsT.sub.ds.sup.mC.sub.ds.- sup.mC.sub.dsT.sub.dsG.sub.dsA.sub.dsT.sub.dsT.sub.dsT.sub.dsG.sub.ksA.sub- .ksG.sub.k 87.4 10765 10780 620 570854 A.sub.ksA.sub.ksG.sub.ksA.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsT.sub.d- s.sup.mC.sub.ds.sup.mC.sub.dsT.sub.dsG.sub.dsA.sub.dsT.sub.dsT.sub.ksT.sub- .ksG.sub.k 60.3 10767 10782 621 570855 .sup.mC.sub.ks.sup.mC.sub.ksA.sub.ksA.sub.dsG.sub.dsA.sub.ds.sup.mC- .sub.ds.sup.mC.sub.dsT.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsT.sub.dsG.sub.dsA- .sub.ksT.sub.ksT.sub.k 61.4 10769 10784 622 570856 .sup.mC.sub.ksT.sub.ksG.sub.ks.sup.mC.sub.dsT.sub.dsT.sub.ds.sup.mC- .sub.ds.sup.mC.sub.dsA.sub.dsA.sub.dsG.sub.dsA.sub.ds.sup.mC.sub.ds.sup.mC- .sub.ksT.sub.ks.sup.mC.sub.k 40.4 10775 10790 623 570857 A.sub.ksG.sub.ks.sup.mC.sub.ksT.sub.dsG.sub.ds.sup.mC.sub.dsT.sub.d- sT.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsA.sub.dsA.sub.dsG.sub.dsA.sub.ks.sup.- mC.sub.ks.sup.mC.sub.k 48.5 10777 10792 624 570858 G.sub.ks.sup.mC.sub.ksA.sub.ksG.sub.ds.sup.mC.sub.dsT.sub.dsG.sub.d- s.sup.mC.sub.dsT.sub.dsT.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsA.sub.dsA.sub.k- sG.sub.ksA.sub.k 87.7 10779 10794 625

570859 .sup.mC.sub.ksT.sub.ksG.sub.ksG.sub.dsT.sub.dsG.sub.dsG.sub.dsA.sub- .dsG.sub.dsA.sub.dsA.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsA.sub.ksG.sub.ksA.s- ub.k 92.6 10816 10831 626 570860 .sup.mC.sub.ksT.sub.ks.sup.mC.sub.ksT.sub.dsG.sub.dsG.sub.dsT.sub.d- sG.sub.dsG.sub.dsA.sub.dsG.sub.dsA.sub.dsA.sub.ds.sup.mC.sub.ks.sup.mC.sub- .ksA.sub.k 86.6 10818 10833 627 570861 T.sub.ksT.sub.ks.sup.mC.sub.ksT.sub.ds.sup.mC.sub.dsT.sub.dsG.sub.d- sG.sub.dsT.sub.dsG.sub.dsG.sub.dsA.sub.dsG.sub.dsA.sub.ksA.sub.ks.sup.mC.s- ub.k 82.6 10820 10835 628 570862 G.sub.ksA.sub.ksT.sub.ksT.sub.ds.sup.mC.sub.dsT.sub.ds.sup.mC.sub.d- sT.sub.dsG.sub.dsG.sub.dsT.sub.dsG.sub.dsG.sub.dsA.sub.ksG.sub.ksA.sub.k 76.1 10822 10837 629 570863 A.sub.ks.sup.mC.sub.ksT.sub.ksT.sub.dsA.sub.ds.sup.mC.sub.dsT.sub.d- sG.sub.dsT.sub.dsT.sub.dsT.sub.ds.sup.mC.sub.dsA.sub.dsT.sub.ks.sup.mC.sub- .ks.sup.mC.sub.k 80.6 10981 10996 630 570864 .sup.mC.sub.ksG.sub.ksG.sub.ksA.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.- sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsT.sub.ds.sup.mC.sub.ds.sup.mC.sub- .ds.sup.mC.sub.ds.sup.mC.sub.ksT.sub.ks.sup.mC.sub.k 58.7 11002 11017 631 570865 G.sub.ksA.sub.ks.sup.mC.sub.ksG.sub.dsG.sub.dsA.sub.ds.sup.mC.sub.d- s.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsT.sub.ds.sup.mC.s- ub.ds.sup.mC.sub.ks.sup.mC.sub.ks.sup.mC.sub.k 61.5 11004 11019 632 570866 .sup.mC.sub.ksT.sub.ksG.sub.ksA.sub.ds.sup.mC.sub.dsG.sub.dsG.sub.d- sA.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.s- ub.dsT.sub.ks.sup.mC.sub.ks.sup.mC.sub.k 47.6 11006 11021 633 570867 .sup.mC.sub.ks.sup.mC.sub.ks.sup.mC.sub.ksT.sub.dsG.sub.dsA.sub.ds.- sup.mC.sub.dsG.sub.dsG.sub.dsA.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.s- ub.ds.sup.mC.sub.ks.sup.mC.sub.ksT.sub.k 69.5 11008 11023 634 570868 A.sub.ksA.sub.ksG.sub.ks.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsT- .sub.ds.sup.mC.sub.dsA.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsT.sub.dsT.sub.dsT- .sub.ksT.sub.ks.sup.mC.sub.k 54 11036 11051 635 570869 G.sub.ksG.sub.ksA.sub.ksA.sub.dsG.sub.ds.sup.mC.sub.ds.sup.mC.sub.d- s.sup.mC.sub.dsT.sub.ds.sup.mC.sub.dsA.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsT- .sub.ksT.sub.ksT.sub.k 37.5 11038 11053 636 570870 .sup.mC.sub.ksG.sub.ksG.sub.ksG.sub.dsA.sub.dsA.sub.dsG.sub.ds.sup.- mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsT.sub.ds.sup.mC.sub.dsA.sub.ds.sup.mC- .sub.ks.sup.mC.sub.ksT.sub.k 70.7 11040 11055 637 570871 .sup.mC.sub.ks.sup.mC.sub.ks.sup.mC.sub.ksG.sub.dsG.sub.dsG.sub.dsA- .sub.dsA.sub.dsG.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsT.sub.ds.- sup.mC.sub.ksA.sub.ks.sup.mC.sub.k 71.2 11042 11057 638 570872 .sup.mC.sub.ksA.sub.ks.sup.mC.sub.ks.sup.mC.sub.ds.sup.mC.sub.dsG.s- ub.dsG.sub.dsG.sub.dsA.sub.dsA.sub.dsG.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.- sup.mC.sub.ksT.sub.ks.sup.mC.sub.k 51.6 11044 11059 639 570873 G.sub.ks.sup.mC.sub.ks.sup.mC.sub.ksA.sub.ds.sup.mC.sub.ds.sup.mC.s- ub.ds.sup.mC.sub.dsG.sub.dsG.sub.dsG.sub.dsA.sub.dsA.sub.dsG.sub.ds.sup.mC- .sub.ks.sup.mC.sub.ks.sup.mC.sub.k 45.8 11046 11061 640 570874 A.sub.ks.sup.mC.sub.ksG.sub.ks.sup.mC.sub.ds.sup.mC.sub.dsA.sub.ds.- sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsG.sub.dsG.sub.dsG.sub.dsA.sub.dsA- .sub.ksG.sub.ks.sup.mC.sub.k 31.8 11048 11063 641 570875 .sup.mCkT.sub.ksG.sub.ksT.sub.dsT.sub.ds.sup.mC.sub.dsA.sub.dsG.sub- .dsG.sub.dsA.sub.dsA.sub.dsG.sub.dsT.sub.ds.sup.mC.sub.ks.sup.mC.sub.ks.su- p.mC.sub.k 14.3 11082 11097 642 570876 T.sub.ksT.sub.ks.sup.mC.sub.ksT.sub.dsG.sub.dsT.sub.dsT.sub.ds.sup.- mC.sub.dsA.sub.dsG.sub.dsG.sub.dsA.sub.dsA.sub.dsG.sub.ksT.sub.ks.sup.mC.s- ub.k 18 11084 11099 643 570877 G.sub.ks.sup.mC.sub.ksT.sub.ksT.sub.ds.sup.mC.sub.dsT.sub.dsG.sub.d- sT.sub.dsT.sub.ds.sup.mC.sub.dsA.sub.dsG.sub.dsG.sub.dsA.sub.ksA.sub.ksG.s- ub.k 44 11086 11101 644

TABLE-US-00015 TABLE 15 Inhibition of human DMPK RNA transcript in HepG2 cells targeting SEQ ID NO: 2 Start Site Stop Site ISIS % Target on Seq on Seq Seq ID No. Sequence Expression ID: 2 ID: 2 No. UTC N/A 100 N/A N/A 445569 .sup.mC.sub.esG.sub.esG.sub.esA.sub.esG.sub.es.sup.mC.sub.dsG.sub.d- sG.sub.dsT.sub.dsT.sub.dsG.sub.dsT.sub.dsG.sub.dsA.sub.dsA.sub.ds.sup.mC.s- ub.esT.sub.esG.sub.esG.sub.es.sup.mC.sub.e 55 13226 13245 24 486178 A.sub.ks.sup.mC.sub.ksA.sub.ksA.sub.dsT.sub.dsA.sub.dsA.sub.dsA.sub- .dsT.sub.dsA.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsG.sub.dsA.sub.ksG.sub.ksG.s- ub.k 33.9 13836 13851 23 570647 Gkg.sup.mC.sub.ksT.sub.ksT.sub.dsG.sub.dsG.sub.dsG.sub.ds.sup.mC.su- b.ds.sup.mC.sub.ds.sup.mC.sub.dsA.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.sup.m- C.sub.ks.sup.mC.sub.ksT.sub.k 80.3 5718 5733 645 570648 A.sub.ksG.sub.ksG.sub.ks.sup.mC.sub.dsT.sub.dsT.sub.dsG.sub.dsG.sub- .dsG.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsA.sub.ds.sup.mC.sub.k- s.sup.mC.sub.ks.sup.mC.sub.k 92.3 5720 5735 646 570649 .sup.mC.sub.ksG.sub.ksA.sub.ksG.sub.dsG.sub.ds.sup.mC.sub.dsT.sub.d- sT.sub.dsG.sub.dsG.sub.dsG.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.k- sA.sub.ks.sup.mC.sub.k 100.7 5722 5737 647 570650 A.sub.ksG.sub.ks.sup.mC.sub.ksG.sub.dsA.sub.dsG.sub.dsG.sub.ds.sup.- mC.sub.dsT.sub.dsT.sub.dsG.sub.dsG.sub.dsG.sub.ds.sup.mC.sub.ks.sup.mC.sub- .ks.sup.mC.sub.k 75.8 5724 5739 648 570651 A.sub.ksG.sub.ksA.sub.ksG.sub.ds.sup.mC.sub.dsG.sub.dsA.sub.dsG.sub- .dsG.sub.ds.sup.mC.sub.dsT.sub.dsT.sub.dsG.sub.dsG.sub.ksG.sub.ks.sup.mC.s- ub.k 99.8 5726 5741 649 570652 G.sub.ks.sup.mC.sub.ksA.sub.ksG.sub.dsA.sub.dsG.sub.ds.sup.mC.sub.d- sG.sub.dsA.sub.dsG.sub.dsG.sub.ds.sup.mC.sub.dsT.sub.dsT.sub.ksG.sub.ksG.s- ub.k 135.4 5728 5743 650 570653 G.sub.ksA.sub.ksG.sub.ks.sup.mC.sub.dsA.sub.dsG.sub.dsA.sub.dsG.sub- .ds.sup.mC.sub.dsG.sub.dsA.sub.dsG.sub.dsG.sub.ds.sup.mC.sub.ksT.sub.ksT.s- ub.k 111.5 5730 5745 651 570654 A.sub.ksA.sub.ksA.sub.ksG.sub.dsG.sub.dsA.sub.dsG.sub.ds.sup.mC.sub- .dsA.sub.dsG.sub.dsA.sub.dsG.sub.ds.sup.mC.sub.dsG.sub.ksA.sub.ksG.sub.k 87.5 5734 5749 652 570655 .sup.mC.sub.ksA.sub.ksA.sub.ksA.sub.dsA.sub.dsG.sub.dsG.sub.dsA.sub- .dsG.sub.ds.sup.mC.sub.dsA.sub.dsG.sub.dsA.sub.dsG.sub.ks.sup.mC.sub.ksG.s- ub.k 94.5 5736 5751 653 570656 T.sub.ksG.sub.ksG.sub.ksA.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsA.sub.d- sA.sub.dsA.sub.dsA.sub.dsG.sub.dsG.sub.dsA.sub.dsG.sub.ks.sup.mC.sub.ksA.s- ub.k 75.4 5741 5756 654 570657 .sup.mC.sub.ks.sup.mC.sub.ksT.sub.ksG.sub.dsG.sub.dsA.sub.ds.sup.mC- .sub.ds.sup.mC.sub.dsA.sub.dsA.sub.dsA.sub.dsA.sub.dsG.sub.dsG.sub.ksA.sub- .ksG.sub.k 87.3 5743 5758 655 570658 .sup.mC.sub.ksA.sub.ks.sup.mC.sub.ks.sup.mC.sub.dsT.sub.dsG.sub.dsG- .sub.dsA.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsA.sub.dsA.sub.dsA.sub.dsA.sub.k- sG.sub.ksG.sub.k 93.2 5745 5760 656 570659 .sup.mC.sub.ksG.sub.ks.sup.mC.sub.ksA.sub.ds.sup.mC.sub.ds.sup.mC.s- ub.dsT.sub.dsG.sub.dsG.sub.dsA.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsA.sub.dsA- .sub.ksA.sub.ksA.sub.k 70 5747 5762 657 570660 G.sub.ksA.sub.ks.sup.mC.sub.ks.sup.mC.sub.dsG.sub.ds.sup.mC.sub.dsA- .sub.ds.sup.mC.sub.ds.sup.mC.sub.dsT.sub.dsG.sub.dsG.sub.dsA.sub.ds.sup.mC- .sub.ks.sup.mC.sub.ksA.sub.k 46.4 5750 5765 658 570661 A.sub.ks.sup.mC.sub.ks.sup.mC.sub.ksT.sub.dsT.sub.dsG.sub.dsT.sub.d- sA.sub.dsG.sub.dsT.sub.dsG.sub.dsG.sub.dsA.sub.ds.sup.mC.sub.ksG.sub.ksA.s- ub.k 44 5951 5966 659 570662 T.sub.ks.sup.mC.sub.ksA.sub.ks.sup.mC.sub.ds.sup.mC.sub.dsT.sub.dsT- .sub.dsG.sub.dsT.sub.dsA.sub.dsG.sub.dsT.sub.dsG.sub.dsG.sub.ksA.sub.ks.su- p.mC.sub.k 76.8 5953 5968 660 570663 Gkc.sup.mC.sub.ksT.sub.ks.sup.mC.sub.dsA.sub.ds.sup.mC.sub.ds.sup.m- C.sub.dsT.sub.dsT.sub.dsG.sub.dsT.sub.dsA.sub.dsG.sub.dsT.sub.ksG.sub.ksG.- sub.k 69.5 5955 5970 661 570664 G.sub.ksG.sub.ksA.sub.ksG.sub.dsA.sub.dsG.sub.dsG.sub.dsA.sub.dsG.s- ub.dsG.sub.ds.sup.mC.sub.dsG.sub.dsA.sub.dsT.sub.ksA.sub.ksG.sub.k 88.2 6015 6030 662 570665 A.sub.ksG.sub.ksG.sub.ksG.sub.dsA.sub.dsG.sub.dsA.sub.dsG.sub.dsG.s- ub.dsA.sub.dsG.sub.dsG.sub.ds.sup.mC.sub.dsG.sub.ksA.sub.ksT.sub.k 96.9 6017 6032 663 570666 .sup.mC.sub.ksT.sub.ks.sup.mC.sub.ks.sup.mC.sub.dsT.sub.dsG.sub.ds.- sup.mC.sub.dsT.sub.ds.sup.mC.sub.dsA.sub.dsG.sub.dsA.sub.dsG.sub.dsG.sub.k- sG.sub.ksA.sub.k 74.7 6028 6043 664 570667 G.sub.ksT.sub.ksgsk.sup.mC.sub.dsT.sub.ds.sup.mC.sub.ds.sup.mC.sub.- dsT.sub.dsG.sub.ds.sup.mC.sub.dsT.sub.ds.sup.mC.sub.dsA.sub.dsG.sub.ksA.su- b.ksG.sub.k 77.5 6031 6046 665 570668 A.sub.ksG.sub.ksG.sub.ksT.sub.dsG.sub.ds.sup.mC.sub.dsT.sub.ds.sup.- mC.sub.ds.sup.mC.sub.dsT.sub.dsG.sub.ds.sup.mC.sub.dsT.sub.ds.sup.mC.sub.k- sA.sub.ksG.sub.k 76.7 6033 6048 666 570669 A.sub.ksG.sub.ksA.sub.ksG.sub.dsG.sub.dsT.sub.dsG.sub.ds.sup.mC.sub- .dsT.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsT.sub.dsG.sub.ds.sup.mC.sub.ksT.sub- .ks.sup.mC.sub.k 43.3 6035 6050 667 570670 A.sub.ksG.sub.ksA.sub.ksG.sub.dsA.sub.dsG.sub.dsG.sub.dsT.sub.dsG.s- ub.ds.sup.mC.sub.dsT.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsT.sub.ksG.sub.ks.su- p.mC.sub.k 27.1 6037 6052 668 570671 A.sub.ks.sup.mC.sub.ks.sup.mC.sub.ks.sup.mC.sub.ds.sup.mC.sub.dsG.s- ub.ds.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.d- sG.sub.ds.sup.mC.sub.dsT.sub.ks.sup.mC.sub.ksA.sub.k 42.6 6291 6306 669 570672 .sup.mC.sub.ksT.sub.ksA.sub.ks.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.s- ub.ds.sup.mC.sub.dsG.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.sup.- mC.sub.ds.sup.mC.sub.dsG.sub.ks.sup.mC.sub.ksT.sub.k 44.9 6293 6308 670 570673 A.sub.ks.sup.mC.sub.ks.sup.mC.sub.ksT.sub.dsA.sub.ds.sup.mC.sub.ds.- sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsG.sub.ds.sup.mC.sub.ds.sup.mC.sub- .ds.sup.mC.sub.ds.sup.mC.sub.ks.sup.mC.sub.ksG.sub.k 36.6 6295 6310 671 570674 G.sub.ksT.sub.ksA.sub.ks.sup.mC.sub.ds.sup.mC.sub.dsT.sub.dsA.sub.d- s.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsG.sub.ds.sup.mC.s- ub.ds.sup.mC.sub.ks.sup.mC.sub.ks.sup.mC.sub.k 52 6297 6312 672 570675 A.sub.ksG.sub.ksG.sub.ksTdaA.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsT.su- b.dsA.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsG.sub.- ks.sup.mC.sub.ks.sup.mC.sub.k 56.4 6299 6314 673 570676 G.sub.ksG.sub.ksG.sub.ksA.sub.dsG.sub.dsG.sub.dsT.sub.dsT.sub.ds.su- p.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsG.sub.ds.sup.mC.sub.dsA.sub.ksG.sub- .ks.sup.mC.sub.k 51.4 6329 6344 674 570677 G.sub.ksT.sub.ks.sup.mC.sub.ks.sup.mC.sub.dsT.sub.dsT.sub.dsA.sub.d- s.sup.mC.sub.dsT.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsA.sub.dsA.sub.ds.sup.mC- .sub.ksT.sub.ksT.sub.k 28 6360 6375 675 570678 .sup.mC.sub.ksT.sub.ksG.sub.ksT.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsT- .sub.dsT.sub.dsA.sub.ds.sup.mC.sub.dsT.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsA- .sub.ksA.sub.ks.sup.mC.sub.k 33.6 6362 6377 676 570679 .sup.mC.sub.ksA.sub.ks.sup.mC.sub.ksT.sub.dsG.sub.dsT.sub.ds.sup.mC- .sub.ds.sup.mC.sub.dsT.sub.dsT.sub.dsA.sub.ds.sup.mC.sub.dsT.sub.ds.sup.mC- .sub.ks.sup.mC.sub.ksA.sub.k 7.9 6364 6379 677 570680 G.sub.ksG.sub.ks.sup.mC.sub.ksA.sub.ds.sup.mC.sub.dsT.sub.dsG.sub.d- sT.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsT.sub.dsT.sub.dsA.sub.ds.sup.mC.sub.k- sT.sub.ks.sup.mC.sub.k 20.2 6366 6381 678 570681 T.sub.ksA.sub.ksG.sub.ksG.sub.ds.sup.mC.sub.dsA.sub.ds.sup.mC.sub.d- sT.sub.dsG.sub.dsT.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsT.sub.dsT.sub.ksA.sub- .ks.sup.mC.sub.k 38.3 6368 6383 679 570682 G.sub.ksG.sub.ksT.sub.ksA.sub.dsG.sub.dsG.sub.ds.sup.mC.sub.dsA.sub- .ds.sup.mC.sub.dsT.sub.dsG.sub.dsT.sub.ds.sup.mC.sub.ds.sup.mC.sub.ksT.sub- .ksT.sub.k 13.9 6370 6385 680 570683 G.sub.ksT.sub.ks.sup.mC.sub.ksA.sub.ds.sup.mC.sub.dsT.sub.dsG.sub.d- s.sup.mC.sub.dsT.sub.dsG.sub.dsG.sub.dsG.sub.dsT.sub.ds.sup.mC.sub.ks.sup.- mC.sub.ksT.sub.k 29 6445 6460 681 570684 G.sub.ksG.sub.ksT.sub.ks.sup.mC.sub.dsA.sub.ds.sup.mC.sub.dsT.sub.d- sG.sub.ds.sup.mC.sub.dsT.sub.dsG.sub.dsG.sub.dsG.sub.dsT.sub.ks.sup.mC.sub- .ks.sup.mC.sub.k 21.3 6446 6461 43 570685 A.sub.ksG.sub.ksG.sub.ksT.sub.ds.sup.mC.sub.dsA.sub.ds.sup.mC.sub.d- sT.sub.dsG.sub.ds.sup.mC.sub.dsT.sub.dsG.sub.dsG.sub.dsG.sub.ksT.sub.ks.su- p.mC.sub.k 16.9 6447 6462 682 570686 .sup.mC.sub.ksT.sub.ksA.sub.ksG.sub.dsG.sub.dsT.sub.ds.sup.mC.sub.d- sA.sub.ds.sup.mC.sub.dsT.sub.dsG.sub.ds.sup.mC.sub.dsT.sub.dsG.sub.ksG.sub- .ksG.sub.k 19.6 6449 6464 683 570687 G.sub.ksT.sub.ks.sup.mC.sub.ksT.sub.dsA.sub.dsG.sub.dsG.sub.dsT.sub- .ds.sup.mC.sub.dsA.sub.ds.sup.mC.sub.dsT.sub.dsG.sub.ds.sup.mC.sub.ksT.sub- .ksG.sub.k 15.7 6451 6466 684 570688 A.sub.ksA.sub.ksG.sub.ksT.sub.ds.sup.mC.sub.dsT.sub.dsA.sub.dsG.sub- .dsG.sub.dsT.sub.ds.sup.mC.sub.dsA.sub.ds.sup.mC.sub.dsT.sub.ksG.sub.ks.su- p.mC.sub.k 16.6 6453 6468 685 570689 G.sub.ks.sup.mC.sub.ksA.sub.ks.sup.mC.sub.dsT.sub.ds.sup.mC.sub.ds.- sup.mC.sub.dsA.sub.dsT.sub.dsT.sub.dsG.sub.dsT.sub.ds.sup.mC.sub.dsT.sub.k- s.sup.mC.sub.ksA.sub.k 13.2 6530 6545 686 570690 .sup.mC.sub.ksT.sub.ksG.sub.ks.sup.mC.sub.dsA.sub.ds.sup.mC.sub.dsT- .sub.ds.sup.mC.sub.ds.sup.mC.sub.dsA.sub.dsT.sub.dsT.sub.dsG.sub.dsT.sub.k- s.sup.mC.sub.ksT.sub.k 50.1 6532 6547 687 570691 .sup.mC.sub.ks.sup.mC.sub.ks.sup.mC.sub.ksT.sub.dsG.sub.ds.sup.mC.s- ub.dsA.sub.ds.sup.mC.sub.dsT.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsA.sub.dsT.s- ub.dsT.sub.ksG.sub.ksT.sub.k 48.4 6534 6549 688 570692 .sup.mC.sub.ks.sup.mC.sub.ks.sup.mC.sub.ks.sup.mC.sub.ds.sup.mC.sub- .dsT.sub.dsG.sub.ds.sup.mC.sub.dsA.sub.ds.sup.mC.sub.dsT.sub.ds.sup.mC.sub- .ds.sup.mC.sub.dsA.sub.ksT.sub.ksT.sub.k 74 6536 6551 689 570693 .sup.mC.sub.ksT.sub.ksT.sub.ksG.sub.ds.sup.mC.sub.dsT.sub.dsG.sub.d- sA.sub.dsG.sub.dsT.sub.ds.sup.mC.sub.dsA.sub.dsG.sub.dsG.sub.ksA.sub.ksG.s- ub.k 25.3 6559 6574 690 570694 T.sub.ks.sup.mC.sub.ks.sup.mC.sub.ksT.sub.dsT.sub.dsG.sub.ds.sup.mC- .sub.dsT.sub.dsG.sub.dsA.sub.dsG.sub.dsT.sub.ds.sup.mC.sub.dsA.sub.ksG.sub- .ksG.sub.k 39.5 6561 6576 691 570695 .sup.mC.sub.ksT.sub.ksT.sub.ks.sup.mC.sub.ds.sup.mC.sub.dsT.sub.dsT- .sub.dsG.sub.ds.sup.mC.sub.dsT.sub.dsG.sub.dsA.sub.dsG.sub.dsT.sub.ks.sup.- mC.sub.ksA.sub.k 22.9 6563 6578 692 570696 A.sub.ks.sup.mC.sub.ks.sup.mC.sub.ksT.sub.dsT.sub.ds.sup.mC.sub.ds.- sup.mC.sub.dsT.sub.dsT.sub.dsG.sub.ds.sup.mC.sub.dsT.sub.dsG.sub.dsA.sub.k- sG.sub.ksT.sub.k 52.5 6565 6580 693 570697 G.sub.ksG.sub.ksA.sub.ks.sup.mC.sub.ds.sup.mC.sub.dsT.sub.dsT.sub.d- s.sup.mC.sub.ds.sup.mC.sub.dsT.sub.dsT.sub.dsG.sub.ds.sup.mC.sub.dsT.sub.k- sG.sub.ksA.sub.k 37.6 6567 6582 694 570698 .sup.mC.sub.ksA.sub.ksG.sub.ksG.sub.dsA.sub.ds.sup.mC.sub.ds.sup.mC- .sub.dsT.sub.dsT.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsT.sub.dsT.sub.dsG.sub.k- s.sup.mC.sub.ksT.sub.k 44.2 6569 6584 695 570699 A.sub.ksG.sub.ks.sup.mC.sub.ks.sup.mC.sub.ds.sup.mC.sub.dsT.sub.ds.- sup.mC.sub.ds.sup.mC.sub.dsA.sub.dsG.sub.dsG.sub.dsA.sub.ds.sup.mC.sub.ds.- sup.mC.sub.ksT.sub.ksT.sub.k 26.6 6576 6591 696 570700 TkA.sub.ksG.sub.ks.sup.mC.sub.dsT.sub.ds.sup.mC.sub.ds.sup.mC.sub.d- s.sup.mC.sub.ds.sup.mC.sub.dsA.sub.ds.sup.mC.sub.dsT.sub.ds.sup.mC.sub.ds.- sup.mC.sub.ksA.sub.ksG.sub.k 33.6 6594 6609 697 570701 G.sub.ksA.sub.ksT.sub.ksA.sub.dsG.sub.ds.sup.mC.sub.dsT.sub.ds.sup.- mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsA.sub.ds.sup.mC.sub.dsT- .sub.ks.sup.mC.sub.ks.sup.mC.sub.k 20.4 6596 6611 698 570702 .sup.mC.sub.ksA.sub.ksG.sub.ksA.sub.dsT.sub.dsA.sub.dsG.sub.ds.sup.- mC.sub.dsT.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsA- .sub.ks.sup.mC.sub.ksT.sub.k 33.8 6598 6613 699 570703 .sup.mC.sub.ksT.sub.ks.sup.mC.sub.ksA.sub.dsG.sub.dsA.sub.dsT.sub.d- sA.sub.dsG.sub.ds.sup.mC.sub.dsT.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC- .sub.ks.sup.mC.sub.ksA.sub.k 25.8 6600 6615 700 570704 A.sub.ksG.sub.ks.sup.mC.sub.ksT.sub.ds.sup.mC.sub.dsA.sub.dsG.sub.d- sA.sub.dsT.sub.dsA.sub.dsG.sub.ds.sup.mC.sub.dsT.sub.ds.sup.mC.sub.ks.sup.- mC.sub.ks.sup.mC.sub.k 29.1 6602 6617 701

570705 T.sub.ks.sup.mC.sub.ksA.sub.ksG.sub.ds.sup.mC.sub.dsT.sub.ds.sup.mC- .sub.dsA.sub.dsG.sub.dsA.sub.dsT.sub.dsA.sub.dsG.sub.ds.sup.mC.sub.ksT.sub- .ks.sup.mC.sub.k 47.4 6604 6619 702 570706 T.sub.ks.sup.mC.sub.ksT.sub.ks.sup.mC.sub.dsA.sub.dsG.sub.ds.sup.mC- .sub.dsT.sub.ds.sup.mC.sub.dsA.sub.dsG.sub.dsA.sub.dsT.sub.dsA.sub.ksG.sub- .ks.sup.mC.sub.k 33.4 6606 6621 703 570707 G.sub.ksA.sub.ksG.sub.ksT.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsT.sub.d- s.sup.mC.sub.dsT.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsT.sub.dsG.sub.ds.sup.mC- .sub.ksT.sub.ksT.sub.k 49 6636 6651 704 570708 G.sub.ksG.sub.ksA.sub.ksG.sub.dsG.sub.dsA.sub.dsG.sub.dsT.sub.ds.su- p.mC.sub.ds.sup.mC.sub.dsT.sub.ds.sup.mC.sub.dsT.sub.ds.sup.mC.sub.ks.sup.- mC.sub.ksT.sub.k 79.2 6640 6655 705 570709 G.sub.ksA.sub.ksG.sub.ksGdA.sub.dsG.sub.dsG.sub.dsA.sub.dsG.sub.dsT- .sub.ds.sup.mC.sub.ds.sup.mC.sub.dsT.sub.ds.sup.mC.sub.ksT.sub.ks.sup.mC.s- ub.k 63.3 6642 6657 706 570710 .sup.mC.sub.ksA.sub.ksA.sub.ksA.sub.dsA.sub.dsG.sub.dsG.sub.dsG.sub- .ds.sup.mC.sub.dsA.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsA.sub.k- sG.sub.ksA.sub.k 38.8 6713 6728 707 570711 A.sub.ksG.sub.ks.sup.mC.sub.ksA.sub.dsA.sub.dsA.sub.dsA.sub.dsG.sub- .dsG.sub.dsG.sub.ds.sup.mC.sub.dsA.sub.ds.sup.mC.sub.ds.sup.mC.sub.ks.sup.- mC.sub.ksA.sub.k 13.7 6715 6730 708 570712 G.sub.ksG.sub.ksA.sub.ksT.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC- .sub.ds.sup.mC.sub.dsA.sub.dsG.sub.dsT.sub.dsA.sub.dsT.sub.dsT.sub.ksG.sub- .ksT.sub.k 45.8 6733 6748 709 570713 .sup.mC.sub.ksT.sub.ksG.sub.ksG.sub.dsA.sub.dsT.sub.ds.sup.mC.sub.d- s.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsA.sub.dsG.sub.dsT.sub.dsA.sub.k- sT.sub.ksT.sub.k 45.6 6735 6750 710 570714 T.sub.ksG.sub.ks.sup.mC.sub.ksT.sub.dsG.sub.dsG.sub.dsA.sub.dsT.sub- .ds.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsA.sub.dsG.sub.k- sT.sub.ksA.sub.k 43.6 6737 6752 711 570715 A.sub.ksT.sub.ksT.sub.ks.sup.mC.sub.dsT.sub.ds.sup.mC.sub.dsT.sub.d- sA.sub.dsG.sub.dsA.sub.ds.sup.mC.sub.dsT.sub.dsG.sub.ds.sup.mC.sub.ksA.sub- .ksA.sub.k 18.3 6789 6804 712 570716 T.sub.ksA.sub.ksA.sub.ksT.sub.dsT.sub.ds.sup.mC.sub.dsT.sub.ds.sup.- mC.sub.dsT.sub.dsA.sub.dsG.sub.dsA.sub.ds.sup.mC.sub.dsT.sub.ksG.sub.ks.su- p.mC.sub.k 15.1 6791 6806 713 570717 T.sub.ks.sup.mC.sub.ksT.sub.ksA.sub.dsA.sub.dsT.sub.dsT.sub.ds.sup.- mC.sub.dsT.sub.ds.sup.mC.sub.dsT.sub.dsA.sub.dsG.sub.dsA.sub.ks.sup.mC.sub- .ksT.sub.k 49.9 6793 6808 714 570718 T.sub.ks.sup.mC.sub.ksT.sub.ks.sup.mC.sub.dsTdA.sub.dsA.sub.dsT.sub- .dsT.sub.ds.sup.mC.sub.dsT.sub.ds.sup.mC.sub.dsT.sub.dsA.sub.ksG.sub.ksA.s- ub.k 77.6 6795 6810 715 570719 .sup.mC.sub.ksT.sub.ks.sup.mC.sub.ks.sup.mC.sub.dsA.sub.dsT.sub.dsA- .sub.dsA.sub.dsT.sub.dsT.sub.ds.sup.mC.sub.dsT.sub.ds.sup.mC.sub.dsT.sub.k- sA.sub.ksA.sub.k 42 6804 6819 716 570720 A.sub.ks.sup.mC.sub.ksT.sub.ks.sup.mC.sub.dsT.sub.ds.sup.mC.sub.ds.- sup.mC.sub.dsA.sub.dsT.sub.dsA.sub.dsA.sub.dsT.sub.dsT.sub.ds.sup.mC.sub.k- sT.sub.ks.sup.mC.sub.k 28.5 6807 6822 717 570721 A.sub.ks.sup.mC.sub.ksA.sub.ks.sup.mC.sub.dsT.sub.ds.sup.mC.sub.dsT- .sub.ds.sup.mC.sub.ds.sup.mC.sub.dsA.sub.dsT.sub.dsA.sub.dsA.sub.dsT.sub.k- sT.sub.ks.sup.mC.sub.k 27.4 6809 6824 718 570722 .sup.mC.sub.ks.sup.mC.sub.ksA.sub.ks.sup.mC.sub.dsA.sub.ds.sup.mC.s- ub.dsT.sub.ds.sup.mC.sub.dsT.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsA.sub.dsT.s- ub.dsA.sub.ksA.sub.ksT.sub.k 35.4 6811 6826 719 570723 T.sub.ksG.sub.ks.sup.mC.sub.ks.sup.mC.sub.dsA.sub.ds.sup.mC.sub.dsA- .sub.ds.sup.mC.sub.dsT.sub.ds.sup.mC.sub.dsT.sub.ds.sup.mC.sub.ds.sup.mC.s- ub.dsA.sub.ksT.sub.ksA.sub.k 45 6813 6828 720

TABLE-US-00016 TABLE 16 Inhibition of human DMPK RNA transcript in HepG2 cells targeting SEQ ID NO: 2 Start Site Stop Site ISIS % Target on Seq on Seq Seq ID No. Sequence Expression ID: 2 ID: 2 No. UTC N/A 100 N/A N/A 445569 .sup.mC.sub.esG.sub.esG.sub.esA.sub.esG.sub.es.sup.mC.sub.dsG.sub.d- sG.sub.dsT.sub.dsT.sub.dsG.sub.dsT.sub.dsG.sub.dsA.sub.dsA.sub.ds.sup.mC.s- ub.esT.sub.esG.sub.esG.sub.es.sup.mC.sub.e 33.9 13226 13245 24 486178 A.sub.ks.sup.mC.sub.ksA.sub.ksA.sub.dsT.sub.dsA.sub.dsA.sub.dsA.sub- .dsT.sub.dsA.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsG.sub.dsA.sub.ksG.sub.ksG.s- ub.k 21.5 13836 13851 23 570339 .sup.mC.sub.ks.sup.mC.sub.ks.sup.mC.sub.ksA.sub.dsT.sub.dsG.sub.ds.- sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsA.sub.dsT.sub.ds.sup.mC.sub.ds.su- p.mC.sub.dsT.sub.ksG.sub.ks.sup.mC.sub.k 56.2 1534 1549 721 570340 G.sub.ksG.sub.ksA.sub.ks.sup.mC.sub.dsA.sub.dsG.sub.dsA.sub.dsG.sub- .dsA.sub.dsA.sub.dsA.sub.dsT.sub.dsG.sub.dsT.sub.ksT.sub.ksG.sub.k 46.7 1597 1612 722 570341 G.sub.ksG.sub.ks.sup.mC.sub.ksA.sub.dsT.sub.dsA.sub.dsG.sub.dsG.sub- .dsA.sub.ds.sup.mC.sub.dsA.sub.dsG.sub.dsA.sub.dsG.sub.ksA.sub.ksA.sub.k 35.6 1603 1618 723 570342 G.sub.ksT.sub.ksG.sub.ksG.sub.es.sup.mC.sub.dsA.sub.dsT.sub.dsA.sub- .dsG.sub.dsG.sub.dsA.sub.ds.sup.mC.sub.dsA.sub.dsG.sub.ksA.sub.ksG.sub.k 34.8 1605 1620 724 570343 T.sub.ksG.sub.ksG.sub.ksT.sub.dsG.sub.dsG.sub.ds.sup.mC.sub.dsA.sub- .dsT.sub.dsA.sub.dsG.sub.dsG.sub.dsA.sub.ds.sup.mC.sub.ksA.sub.ksG.sub.k 60.3 1607 1622 725 570344 .sup.mC.sub.ksT.sub.ksT.sub.ksA.sub.ds.sup.mC.sub.dsT.sub.ds.sup.mC- .sub.dsT.sub.dsG.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.s- ub.dsT.sub.ks.sup.mC.sub.ks.sup.mC.sub.k 49.6 1627 1642 726 570345 A.sub.ks.sup.mC.sub.ks.sup.mC.sub.ksT.sub.dsT.sub.dsA.sub.ds.sup.mC- .sub.dsT.sub.ds.sup.mC.sub.dsT.sub.dsG.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.- sup.mC.sub.ks.sup.mC.sub.ksT.sub.k 48.6 1629 1644 727 570346 T.sub.ksG.sub.ksA.sub.ks.sup.mC.sub.ds.sup.mC.sub.dsT.sub.dsT.sub.d- sA.sub.ds.sup.mC.sub.dsT.sub.ds.sup.mC.sub.dsT.sub.dsG.sub.ds.sup.mC.sub.k- s.sup.mC.sub.ks.sup.mC.sub.k 36.8 1631 1646 728 570347 G.sub.ks.sup.mC.sub.ksT.sub.ksG.sub.dsA.sub.ds.sup.mC.sub.ds.sup.mC- .sub.dsT.sub.dsT.sub.dsA.sub.ds.sup.mC.sub.dsT.sub.ds.sup.mC.sub.dsT.sub.k- sG.sub.ks.sup.mC.sub.k 53.5 1633 1648 729 570348 .sup.mC.sub.ksT.sub.ksG.sub.ks.sup.mC.sub.dsT.sub.dsG.sub.dsA.sub.d- s.sup.mC.sub.ds.sup.mC.sub.dsT.sub.dsT.sub.dsA.sub.ds.sup.mC.sub.dsT.sub.k- s.sup.mC.sub.ksT.sub.k 59 1635 1650 730 570349 .sup.mC.sub.ksT.sub.ks.sup.mC.sub.ksT.sub.dsG.sub.ds.sup.mC.sub.dsT- .sub.dsG.sub.dsA.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsT.sub.dsT.sub.dsA.sub.k- s.sup.mC.sub.ksT.sub.k 70.8 1637 1652 731 570350 G.sub.ks.sup.mC.sub.ks.sup.mC.sub.ksT.sub.ds.sup.mC.sub.dsT.sub.dsG- .sub.ds.sup.mC.sub.dsT.sub.dsG.sub.dsA.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsT- .sub.ksT.sub.ksA.sub.k 54 1639 1654 732 570351 .sup.mC.sub.ks.sup.mC.sub.ksA.sub.ksT.sub.dsG.sub.dsG.sub.ds.sup.mC- .sub.dsT.sub.ds.sup.mC.sub.dsT.sub.dsG.sub.dsA.sub.dsG.sub.dsT.sub.ks.sup.- mC.sub.ksA.sub.k 52.6 1666 1681 733 570352 A.sub.ksG.sub.ks.sup.mC.sub.ks.sup.mC.sub.dsA.sub.dsT.sub.dsG.sub.d- sG.sub.ds.sup.mC.sub.dsT.sub.ds.sup.mC.sub.dsT.sub.dsG.sub.dsA.sub.ksG.sub- .ksT.sub.k 60.7 1668 1683 734 570353 T.sub.ksA.sub.ksA.sub.ksG.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsA.sub.d- sT.sub.dsG.sub.dsG.sub.ds.sup.mC.sub.dsT.sub.ds.sup.mC.sub.dsT.sub.ksG.sub- .ksA.sub.k 82.3 1670 1685 735 570354 T.sub.ksA.sub.ksG.sub.ks.sup.mC.sub.ds.sup.mC.sub.dsT.sub.dsG.sub.d- s.sup.mC.sub.dsT.sub.dsG.sub.dsT.sub.dsG.sub.dsA.sub.ds.sup.mC.sub.ksT.sub- .ks.sup.mC.sub.k 40.8 1687 1702 736 570355 A.sub.ksT.sub.ksG.sub.ksG.sub.dsG.sub.dsA.sub.dsG.sub.dsG.sub.ds.su- p.mC.sub.dsT.sub.dsG.sub.dsT.sub.dsT.sub.dsG.sub.ksG.sub.ks.sup.mC.sub.k 90.7 1707 1722 737 570356 .sup.mC.sub.ks.sup.mC.sub.ksA.sub.ksT.sub.dsG.sub.dsG.sub.dsG.sub.d- sA.sub.dsG.sub.dsG.sub.ds.sup.mC.sub.dsT.sub.dsG.sub.dsT.sub.ksT.sub.ksG.s- ub.k 73.9 1709 1724 738 570357 G.sub.ksG.sub.ks.sup.mC.sub.ks.sup.mC.sub.dsA.sub.dsT.sub.dsG.sub.d- sG.sub.dsG.sub.dsA.sub.dsG.sub.dsG.sub.ds.sup.mC.sub.dsT.sub.ksG.sub.ksT.s- ub.k 94.9 1711 1726 739 570358 G.sub.ksT.sub.ksG.sub.ks.sup.mC.sub.dsA.sub.dsG.sub.dsA.sub.dsG.sub- .dsA.sub.dsG.sub.dsG.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsA.sub.ksT.sub.ksG.s- ub.k 73.5 1720 1735 740 570359 G.sub.ksA.sub.ksG.sub.ks.sup.mC.sub.dsT.sub.ds.sup.mC.sub.ds.sup.mC- .sub.ds.sup.mC.sub.dsA.sub.dsG.sub.ds.sup.mC.sub.dsA.sub.dsT.sub.dsG.sub.k- sA.sub.ks.sup.mC.sub.k 70.2 1759 1774 741 570360 A.sub.ksG.sub.ksG.sub.ksG.sub.dsA.sub.dsG.sub.ds.sup.mC.sub.dsT.sub- .ds.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsA.sub.dsG.sub.ds.sup.mC.sub.k- sA.sub.ksT.sub.k 56.1 1762 1777 742 570361 G.sub.ks.sup.mC.sub.ks.sup.mC.sub.ksA.sub.dsT.sub.dsA.sub.dsG.sub.d- sA.sub.dsG.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsA.sub.ds.sup.mC- .sub.ksT.sub.ksT.sub.k 54.9 1799 1814 743 570362 G.sub.ksG.sub.ksG.sub.ks.sup.mC.sub.ds.sup.mC.sub.ksA.sub.dsT.sub.d- sA.sub.dsG.sub.dsA.sub.dsG.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.k- sA.sub.ks.sup.mC.sub.k 78.1 1801 1816 744 570363 A.sub.ksT.sub.ksG.sub.ks.sup.mC.sub.dsT.sub.dsG.sub.dsG.sub.ds.sup.- mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsT.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsT- .sub.ksG.sub.ksG.sub.k 76.2 1848 1863 745 570364 A.sub.ksG.sub.ks.sup.mC.sub.ksT.sub.dsG.sub.ds.sup.mC.sub.ds.sup.mC- .sub.ds.sup.mC.sub.ds.sup.mC.sub.dsA.sub.dsT.sub.dsG.sub.ds.sup.mC.sub.dsT- .sub.ksG.sub.ksG.sub.k 92.6 1857 1872 746 570365 .sup.mC.sub.ksG.sub.ks.sup.mC.sub.ks.sup.mC.sub.ds.sup.mC.sub.ds.su- p.mC.sub.dsT.sub.dsG.sub.dsG.sub.ds.sup.mC.sub.dsA.sub.dsG.sub.ds.sup.mC.s- ub.dsT.sub.ksG.sub.ks.sup.mC.sub.k 73.6 1867 1882 747 570366 T.sub.ksG.sub.ks.sup.mC.sub.ksG.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.- sup.mC.sub.ds.sup.mC.sub.dsT.sub.dsG.sub.dsG.sub.ds.sup.mC.sub.dsA.sub.dsG- .sub.ks.sup.mC.sub.ksT.sub.k 76.6 1869 1884 748 570367 G.sub.ks.sup.mC.sub.ksT.sub.ksG.sub.ds.sup.mC.sub.dsG.sub.ds.sup.mC- .sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsT.sub.dsG.sub.dsG.sub.ds.- sup.mC.sub.ksA.sub.ksG.sub.k 79.1 1871 1886 749 570368 .sup.mC.sub.ksG.sub.ksG.sub.ks.sup.mC.sub.dsT.sub.dsG.sub.ds.sup.mC- .sub.dsG.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsT.s- ub.dsG.sub.ksG.sub.ks.sup.mC.sub.k 82.9 1873 1888 750 570369 G.sub.ksT.sub.ks.sup.mC.sub.ksG.sub.dsG.sub.ds.sup.mC.sub.dsT.sub.d- sG.sub.ds.sup.mC.sub.dsG.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.- sup.mC.sub.ksT.sub.ksg.sub.k 47.5 1875 1890 751 570370 .sup.mC.sub.ksT.sub.ksG.sub.ksT.sub.ds.sup.mC.sub.dsG.sub.dsG.sub.d- s.sup.mC.sub.dsT.sub.dsG.sub.ds.sup.mC.sub.dsG.sub.ds.sup.mC.sub.ds.sup.mC- .sub.ks.sup.mC.sub.ks.sup.mC.sub.k 79.6 1877 1892 752 570371 G.sub.ks.sup.mC.sub.ks.sup.mC.sub.ksT.sub.dsG.sub.dsT.sub.ds.sup.mC- .sub.dsG.sub.dsG.sub.ds.sup.mC.sub.dsT.sub.dsdG.sub.ds.sup.mC.sub.dsG.sub.- ks.sup.mC.sub.ks.sup.mC.sub.k 58.4 1879 1894 753 570372 .sup.mC.sub.ksT.sub.ksG.sub.ks.sup.mC.sub.ds.sup.mC.sub.dsT.sub.dsG- .sub.dsT.sub.ds.sup.mC.sub.dsG.sub.dsG.sub.ds.sup.mC.sub.dsT.sub.dsG.sub.k- s.sup.mC.sub.dsg.sub.k 49.9 1881 1896 754 570373 A.sub.ks.sup.mC.sub.ks.sup.mC.sub.ksT.sub.dsG.sub.ds.sup.mC.sub.ds.- sup.mC.sub.dsT.sub.dsG.sub.dsT.sub.ds.sup.mC.sub.dsG.sub.dsG.sub.ds.sup.mC- .sub.ksT.sub.ksg.sub.k 27.4 1883 1898 755 570374 A.sub.ks.sup.mC.sub.ksA.sub.ks.sup.mC.sub.ds.sup.mC.sub.dsT.sub.dsG- .sub.ds.sup.mC.sub.ds.sup.mC.sub.dsT.sub.dsG.sub.dsT.sub.ds.sup.mC.sub.dsG- .sub.ksG.sub.ks.sup.mC.sub.k 54.3 1885 1900 756 570375 G.sub.ksA.sub.ksA.sub.ks.sup.mC.sub.dsA.sub.ds.sup.mC.sub.ds.sup.mC- .sub.dsT.sub.dsG.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsT.sub.dsG.sub.dsT.sub.k- s.sup.mC.sub.ksg.sub.k 50.5 1887 1902 757 570376 .sup.mC.sub.ks.sup.mC.sub.ksG.sub.ksA.sub.dsA.sub.ds.sup.mC.sub.dsA- .sub.ds.sup.mC.sub.ds.sup.mC.sub.dsT.sub.dsG.sub.ds.sup.mC.sub.ds.sup.mC.s- ub.dsT.sub.ksG.sub.kst.sub.k 57.7 1889 1904 758 570377 .sup.mC.sub.ksG.sub.ks.sup.mC.sub.ks.sup.mC.sub.dsG.sub.dsA.sub.dsA- .sub.ds.sup.mC.sub.dsA.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsT.sub.dsG.sub.ds.- sup.mC.sub.ks.sup.mC.sub.kst.sub.k 69.3 1891 1906 759 570378 .sup.mC.sub.ks.sup.mC.sub.ksT.sub.ksG.sub.dsG.sub.dsG.sub.ds.sup.mC- .sub.dsA.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsT.sub.dsG.sub.dsT.sub.dsT.sub.k- sG.sub.ksg.sub.k 188.2 1925 1940 760 570379 G.sub.ksT.sub.ksG.sub.ks.sup.mC.sub.ds.sup.mC.sub.dsT.sub.dsG.sub.d- sG.sub.dsG.sub.ds.sup.mC.sub.dsA.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsT.sub.k- sG.sub.kst.sub.k 111.5 1928 1943 761 570380 .sup.mC.sub.ksG.sub.ks.sup.mC.sub.ks.sup.mC.sub.ds.sup.mC.sub.dsT.s- ub.ds.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsA.sub.dsG.sub.dsT.sub.dsG.s- ub.ds.sup.mC.sub.ks.sup.mC.sub.kst.sub.k 78 1938 1953 762 570381 A.sub.ks.sup.mC.sub.ks.sup.mC.sub.ksG.sub.ds.sup.mC.sub.ds.sup.mC.s- ub.ds.sup.mC.sub.dsT.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsA.sub- .dsG.sub.dsT.sub.ksG.sub.ks.sup.mC.sub.k 74.9 1940 1955 763 570382 T.sub.ks.sup.mC.sub.ksA.sub.ks.sup.mC.sub.ds.sup.mC.sub.dsG.sub.ds.- sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsT.sub.ds.sup.mC.sub.ds.sup.mC.sub- .ds.sup.mC.sub.dsA.sub.ksG.sub.kst.sub.k 71.6 1942 1957 764 570383 A.sub.ksG.sub.ksT.sub.ks.sup.mC.sub.dsA.sub.ds.sup.mC.sub.ds.sup.mC- .sub.dsG.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsT.sub.ds.sup.mC.s- ub.ds.sup.mC.sub.ks.sup.mC.sub.ksa.sub.k 62.1 1944 1959 765 570384 T.sub.ksG.sub.ksA.sub.ksG.sub.dsT.sub.ds.sup.mC.sub.dsA.sub.ds.sup.- mC.sub.ds.sup.mC.sub.dsG.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsT- .sub.ks.sup.mC.sub.ks.sup.mC.sub.k 65.6 1946 1961 766 570385 .sup.mC.sub.ksG.sub.ksT.sub.ksG.sub.dsA.sub.dsG.sub.dsT.sub.ds.sup.- mC.sub.dsA.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsG.sub.ds.sup.mC.sub.ds.sup.mC- .sub.ks.sup.mC.sub.kst.sub.k 37.3 1948 1963 767 570386 .sup.mC.sub.ksA.sub.ksA.sub.ksA.sub.dsG.sub.ds.sup.mC.sub.dsT.sub.d- sG.sub.dsG.sub.dsT.sub.dsT.sub.ds.sup.mC.sub.dsT.sub.ds.sup.mC.sub.ks.sup.- mC.sub.ks.sup.mC.sub.k 30.5 1974 1989 768 570387 T.sub.ksG.sub.ks.sup.mC.sub.ksA.sub.dsA.sub.dsA.sub.dsG.sub.ds.sup.- mC.sub.dsT.sub.dsG.sub.dsG.sub.dsT.sub.dsT.sub.ds.sup.mC.sub.ksT.sub.ks.su- p.mC.sub.k 35.8 1976 1991 769 570388 T.sub.ks.sup.mC.sub.ksT.sub.ksG.sub.ds.sup.mC.sub.dsA.sub.dsA.sub.d- sA.sub.dsA.sub.dsG.sub.ds.sup.mC.sub.dsT.sub.dsG.sub.dsG.sub.dsT.sub.ksT.s- ub.ks.sup.mC.sub.k 30.1 1978 1993 770 570389 T.sub.ksG.sub.ksT.sub.ks.sup.mC.sub.dsT.sub.dsG.sub.ds.sup.mC.sub.d- sA.sub.dsA.sub.dsA.sub.dsG.sub.ds.sup.mC.sub.dsT.sub.dsG.sub.ksG.sub.kst.s- ub.k 50.1 1980 1995 771 570390 .sup.mC.sub.ks.sup.mC.sub.ksT.sub.ksG.sub.dsT.sub.ds.sup.mC.sub.dsT- .sub.dsG.sub.ds.sup.mC.sub.dsA.sub.dsA.sub.dsA.sub.dsG.sub.ds.sup.mC.sub.k- sT.sub.ksg.sub.k 36 1982 1997 772 570391 .sup.mC.sub.ksG.sub.ks.sup.mC.sub.ks.sup.mC.sub.dsT.sub.dsG.sub.dsT- .sub.ds.sup.mC.sub.dsT.sub.dsG.sub.ds.sup.mC.sub.dsA.sub.dsA.sub.dsA.sub.k- sG.sub.ks.sup.mC.sub.k 31.1 1984 1999 773 570392 T.sub.ksT.sub.ksG.sub.ksT.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC- .sub.dsT.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsT.sub.dsG.sub.dsG.sub.dsA.sub.k- sT.sub.ks.sup.mC.sub.k 62.9 2022 2037 774 570393 A.sub.ksG.sub.ksT.sub.ksT.sub.dsG.sub.dsT.sub.ds.sup.mC.sub.ds.sup.- mC.sub.ds.sup.mC.sub.dsT.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsT.sub.dsG.sub.k- sG.sub.ksa.sub.k 57.1 2024 2039 775 570394 A.sub.ksA.sub.ksA.sub.ksG.sub.dsT.sub.dsT.sub.dsG.sub.dsT.sub.ds.su- p.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsT.sub.ds.sup.mC.sub.ds.sup.mC.sub.k- sT.sub.ksg.sub.k 56.2 2026 2041 776 570395 .sup.mC.sub.ks.sup.mC.sub.ksA.sub.ksA.sub.dsA.sub.dsG.sub.dsT.sub.d- sT.sub.dsG.sub.dsT.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsT.sub.k- s.sup.mC.sub.ks.sup.mC.sub.k 48.9 2028 2043 777 570396 A.sub.ks.sup.mC.sub.ks.sup.mC.sub.ks.sup.mC.sub.dsA.sub.dsA.sub.dsA- .sub.dsG.sub.dsT.sub.dsT.sub.dsG.sub.dsT.sub.ds.sup.mC.sub.ds.sup.mC.sub.k- s.sup.mC.sub.kst.sub.k 59.9 2030 2045 778

570397 G.sub.ksA.sub.ksA.sub.ks.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsA- .sub.dsA.sub.dsA.sub.dsG.sub.dsT.sub.dsT.sub.dsG.sub.dsT.sub.ks.sup.mC.sub- .ks.sup.mC.sub.k 47.9 2032 2047 779 570398 G.sub.ksA.sub.ksA.sub.ksG.sub.dsA.sub.dsA.sub.ds.sup.mC.sub.ds.sup.- mC.sub.ds.sup.mC.sub.dsA.sub.dsA.sub.dsA.sub.dsG.sub.dsT.sub.ksT.sub.ksg.s- ub.k 60 2035 2050 780 570399 .sup.mC.sub.ks.sup.mC.sub.ksA.sub.ksG.sub.dsA.sub.dsA.sub.dsG.sub.d- sA.sub.dsA.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsA.sub.dsA.sub.k- sA.sub.ksg.sub.k 51.2 2038 2053 781 570400 .sup.mCkA.sub.ks.sup.mC.sub.ks.sup.mC.sub.ds.sup.mC.sub.dsA.sub.dsG- .sub.dsA.sub.dsA.sub.dsG.sub.dsA.sub.dsA.sub.ds.sup.mC.sub.ds.sup.mC.sub.k- s.sup.mC.sub.ksa.sub.k 51.1 2041 2056 782 570401 G.sub.ks.sup.mC.sub.ksA.sub.ksG.sub.dsA.sub.dsA.sub.ds.sup.mC.sub.d- s.sup.mC.sub.dsT.sub.dsA.sub.ds.sup.mC.sub.dsA.sub.dsA.sub.dsA.sub.ksA.sub- .ksg.sub.k 44.9 2066 2081 783 570402 G.sub.ksT.sub.ksG.sub.ks.sup.mC.sub.dsA.sub.dsG.sub.dsA.sub.dsA.sub- .ds.sup.mC.sub.ds.sup.mC.sub.dsT.sub.dsA.sub.ds.sup.mC.sub.dsA.sub.ksA.sub- .ksA.sub.k 53 2068 2083 784 570403 G.sub.ksG.sub.ksG.sub.ksT.sub.dsG.sub.ds.sup.mC.sub.dsA.sub.dsG.sub- .dsA.sub.dsAdg.sup.mC.sub.ds.sup.mC.sub.dsT.sub.dsA.sub.ks.sup.mC.sub.ksA.- sub.k 51.5 2070 2085 785 570404 G.sub.ksT.sub.ksG.sub.ksG.sub.dsG.sub.dsT.sub.dsG.sub.ds.sup.mC.sub- .dsA.sub.dsG.sub.dsA.sub.dsA.sub.ds.sup.mC.sub.ds.sup.mC.sub.ksT.sub.ksA.s- ub.k 57.4 2072 2087 786 570405 .sup.mC.sub.ks.sup.mC.sub.ksA.sub.ks.sup.mC.sub.dsA.sub.ds.sup.mC.s- ub.dsG.sub.dsG.sub.ds.sup.mC.sub.dsT.sub.ds.sup.mC.sub.dsA.sub.dsT.sub.dsA- .sub.ksG.sub.ksG.sub.k 54.3 2116 2131 787 570406 A.sub.ks.sup.mC.sub.ks.sup.mC.sub.ks.sup.mC.sub.dsA.sub.ds.sup.mC.s- ub.dsA.sub.ds.sup.mC.sub.dsG.sub.dsG.sub.ds.sup.mC.sub.dsT.sub.ds.sup.mC.s- ub.dsA.sub.ksT.sub.ksA.sub.k 43.6 2118 2133 788 570407 T.sub.ksG.sub.ksA.sub.ks.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsA- .sub.ds.sup.mC.sub.dsA.sub.ds.sup.mC.sub.dsG.sub.dsG.sub.ds.sup.mC.sub.dsT- .sub.ks.sup.mC.sub.ksA.sub.k 44 2120 2135 789 570408 G.sub.ks.sup.mC.sub.ksT.sub.ksG.sub.dsA.sub.ds.sup.mC.sub.ds.sup.mC- .sub.ds.sup.mC.sub.dsA.sub.ds.sup.mC.sub.dsA.sub.ds.sup.mC.sub.dsG.sub.dsG- .sub.ks.sup.mC.sub.ksT.sub.k 56.5 2122 2137 790 570409 T.sub.ksG.sub.ksG.sub.ks.sup.mC.sub.dsT.sub.dsG.sub.dsA.sub.ds.sup.- mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsA.sub.ds.sup.mC.sub.dsA.sub.ds.sup.mC- .sub.ksG.sub.ksG.sub.k 54.8 2124 2139 791 570410 G.sub.ksG.sub.ksT.sub.ksG.sub.dsG.sub.ds.sup.mC.sub.dsT.sub.dsG.sub- .dsA.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsA.sub.ds.sup.mC.sub.k- sA.sub.ks.sup.mC.sub.k 46.8 2126 2141 792 570411 A.sub.ksT.sub.ksG.sub.ksG.sub.dsT.sub.dsG.sub.dsG.sub.ds.sup.mC.sub- .dsT.sub.dsG.sub.dsA.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.ksA.sub- .ks.sup.mC.sub.k 73.8 2128 2143 793 570412 G.sub.ksA.sub.ksA.sub.ksT.sub.dsG.sub.dsG.sub.dsT.sub.dsG.sub.dsG.s- ub.ds.sup.mC.sub.dsT.sub.dsG.sub.dsA.sub.ds.sup.mC.sub.ks.sup.mC.sub.ks.su- p.mC.sub.k 43.5 2130 2145 794 570413 .sup.mC.sub.ksT.sub.ksA.sub.ksA.sub.dsA.sub.dsG.sub.dsG.sub.dsA.sub- .ds.sup.mC.sub.dsG.sub.ds.sup.mC.sub.dsA.sub.dsG.sub.dsG.sub.ksG.sub.ksA.s- ub.k 54.4 2159 2174 795 570414 A.sub.ksA.sub.ks.sup.mC.sub.ksT.sub.dsA.sub.dsA.sub.dsA.sub.dsG.sub- .dsG.sub.dsA.sub.ds.sup.mC.sub.dsG.sub.ds.sup.mC.sub.dsA.sub.ksG.sub.ksg.s- ub.k 49.1 2161 2176 796 570415 G.sub.ksA.sub.ksG.sub.ksA.sub.dsA.sub.ds.sup.mC.sub.dsT.sub.dsA.sub- .dsA.sub.dsA.sub.dsG.sub.dsG.sub.dsA.sub.ds.sup.mC.sub.ksG.sub.ks.sup.mC.s- ub.k 35.4 2164 2179 797

TABLE-US-00017 TABLE 17 Inhibition of human DMPK RNA transcript in HepG2 cells targeting SEQ ID NO: 2 Start Site Stop Site ISIS % Target on Seq on Seq Seq ID No. Sequence Expression ID: 2 ID: 2 No. UTC N/A 100 N/A N/A 445569 .sup.mC.sub.esG.sub.esGsAsG.sub.es.sup.mC.sub.dsG.sub.dsG.sub.dsT.s- ub.dsT.sub.dsG.sub.dsT.sub.dsG.sub.dsA.sub.dsA.sub.ds.sup.mC.sub.esT.sub.e- sG.sub.esG.sub.es.sup.mC.sub.e 41.4 13226 13245 24 486178 A.sub.ks.sup.mC.sub.ksA.sub.ksA.sub.dsT.sub.dsA.sub.dsA.sub.dsA.sub- .dsT.sub.dsA.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsG.sub.dsA.sub.ksG.sub.ksG.s- ub.k 24 13836 13851 23 570493 A.sub.ksT.sub.ksT.sub.ksG.sub.dsG.sub.dsTdg.sup.mC.sub.ds.sup.mC.su- b.ds.sup.mC.sub.dsA.sub.dsA.sub.dsG.sub.ds.sup.mC.sub.ds.sup.mC.sub.ks.sup- .mC.sub.ks.sup.mC.sub.k 112.1 3973 3988 798 570494 .sup.mC.sub.ks.sup.mC.sub.ksA.sub.ksT.sub.dsT.sub.dsG.sub.dsG.sub.d- sT.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsA.sub.dsA.sub.dsG.sub.k- s.sup.mC.sub.ks.sup.mC.sub.k 91.3 3975 3990 799 570495 G.sub.ks.sup.mC.sub.ks.sup.mC.sub.ks.sup.mC.sub.dsA.sub.dsT.sub.dsT- .sub.dsG.sub.dsG.sub.dsT.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsA- .sub.ksA.sub.ksG.sub.k 103.4 3977 3992 800 570496 A.sub.ks.sup.mC.sub.ksG.sub.ks.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.s- ub.dsA.sub.dsT.sub.dsT.sub.dsG.sub.dsG.sub.dsT.sub.ds.sup.mC.sub.ds.sup.mC- .sub.ks.sup.mC.sub.ksA.sub.k 67.8 3979 3994 801 570497 .sup.mC.sub.ks.sup.mC.sub.ksA.sub.ks.sup.mC.sub.dsG.sub.ds.sup.mC.s- ub.ds.sup.mC.sub.ds.sup.mC.sub.dsA.sub.dsT.sub.dsT.sub.dsG.sub.dsG.sub.dsT- .sub.ks.sup.mC.sub.ks.sup.mC.sub.k 77.3 3981 3996 802 570498 .sup.mC.sub.ksA.sub.ks.sup.mC.sub.ks.sup.mC.sub.dsA.sub.ds.sup.mC.s- ub.dsG.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsA.sub.dsT.sub.dsT.s- ub.dsG.sub.ksG.sub.ksT.sub.k 98.3 3983 3998 803 570499 A.sub.ksG.sub.ksA.sub.ks.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsA- .sub.dsA.sub.ds.sup.mC.sub.dsT.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsA.sub.ds.- sup.mC.sub.ks.sup.mC.sub.ks.sup.mC.sub.k 63.7 4036 4051 804 570500 T.sub.ks.sup.mC.sub.ksA.sub.ks.sup.mC.sub.ds.sup.mC.sub.dsT.sub.ds.- sup.mC.sub.dsG.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub- .dsT.sub.ds.sup.mC.sub.ksT.sub.ksT.sub.k 43 4181 4196 805 570501 .sup.mC.sub.ks.sup.mC.sub.ksT.sub.ks.sup.mC.sub.dsA.sub.ds.sup.mC.s- ub.ds.sup.mC.sub.dsT.sub.ds.sup.mC.sub.dsG.sub.ds.sup.mC.sub.ds.sup.mC.sub- .ds.sup.mC.sub.ds.sup.mC.sub.ksT.sub.ks.sup.mC.sub.k 38.1 4183 4198 806 570502 A.sub.ksG.sub.ks.sup.mC.sub.ks.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.s- ub.dsT.sub.ds.sup.mC.sub.dsA.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsT.sub.ds.su- p.mC.sub.dsG.sub.ks.sup.mC.sub.ks.sup.mC.sub.k 85.4 4187 4202 807 570503 .sup.mC.sub.ksT.sub.ks.sup.mC.sub.ksA.sub.dsA.sub.dsA.sub.dsG.sub.d- s.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.su- p.mG.sub.dsA.sub.ks.sup.mC.sub.ksG.sub.k 115.8 4210 4225 808 570504 A.sub.ksT.sub.ks.sup.mC.sub.ks.sup.mC.sub.dsT.sub.ds.sup.mC.sub.dsA- .sub.dsA.sub.dsA.sub.dsG.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.- sup.mC.sub.ks.sup.mC.sub.ks.sup.mC.sub.k 114.5 4213 4228 809 570505 G.sub.ksG.sub.ksA.sub.ksT.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsT.sub.d- s.sup.mC.sub.dsAchA.sub.dsA.sub.dsG.sub.ds.sup.mC.sub.ds.sup.mC.sub.ks.sup- .mC.sub.ks.sup.mC.sub.k 88.1 4215 4230 810 570506 G.sub.ks.sup.mC.sub.ksG.sub.ksG.sub.dsA.sub.dsT.sub.ds.sup.mC.sub.d- s.sup.mC.sub.dsT.sub.ds.sup.mC.sub.dsA.sub.dsA.sub.dsA.sub.dsG.sub.ks.sup.- mC.sub.ks.sup.mC.sub.k 93.1 4217 4232 811 570507 G.sub.ks.sup.mC.sub.ksG.sub.ks.sup.mC.sub.dsG.sub.dsG.sub.dsA.sub.d- sT.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsT.sub.ds.sup.mC.sub.dsA.sub.dsA.sub.k- sA.sub.ksG.sub.k 102.9 4219 4234 812 570508 G.sub.ksG.sub.ksG.sub.ks.sup.mC.sub.dsG.sub.ds.sup.mC.sub.dsG.sub.d- sG.sub.dsA.sub.dsT.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsT.sub.ds.sup.mC.sub.k- sA.sub.ksA.sub.k 78.5 4221 4236 813 570509 G.sub.ksA.sub.ksG.sub.ks.sup.mC.sub.dsT.sub.dsG.sub.ds.sup.mC.sub.d- sA.sub.dsG.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsG.sub.dsG.sub.dsA.sub.ksG.sub- .ksA.sub.k 192.2 4239 4254 814 570510 A.sub.ksG.sub.ksG.sub.ksA.sub.dsG.sub.ds.sup.mC.sub.dsT.sub.dsG.sub- .ds.sup.mC.sub.dsA.sub.dsG.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsG.sub.ksG.sub- .ksA.sub.k 219.8 4241 4256 815 570511 .sup.mC.sub.ksG.sub.ksG.sub.ksA.sub.dsG.sub.dsG.sub.dsA.sub.dsG.sub- .ds.sup.mC.sub.dsT.sub.dsG.sub.ds.sup.mC.sub.dsA.sub.dsG.sub.ks.sup.mC.sub- .ks.sup.mC.sub.k 128.6 4244 4259 816 570512 A.sub.ks.sup.mC.sub.ks.sup.mC.sub.ks.sup.mC.sub.dsG.sub.dsG.sub.dsA- .sub.dsG.sub.dsG.sub.dsA.sub.dsG.sub.ds.sup.mC.sub.dsT.sub.dsG.sub.ks.sup.- mC.sub.ksA.sub.k 89.9 4247 4262 817 570513 G.sub.ks.sup.mC.sub.ksA.sub.ks.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.s- ub.dsG.sub.dsG.sub.dsA.sub.dsG.sub.dsG.sub.dsA.sub.dsG.sub.ds.sup.mC.sub.k- sT.sub.ksG.sub.k 96.1 4249 4264 818 570514 G.sub.ksG.sub.ksG.sub.ks.sup.mC.sub.dsA.sub.ds.sup.mC.sub.ds.sup.mC- .sub.ds.sup.mC.sub.dsG.sub.dsG.sub.dsA.sub.dsG.sub.dsG.sub.dsA.sub.ksG.sub- .ks.sup.mC.sub.k 67.8 4251 4266 819 570515 .sup.mC.sub.ksA.sub.ksG.sub.ksG.sub.dsG.sub.ds.sup.mC.sub.dsA.sub.d- s.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsG.sub.dsG.sub.dsA.sub.dsG.sub.k- sGkA.sub.k 64.2 4253 4268 820 570516 T.sub.ksG.sub.ks.sup.mC.sub.ksA.sub.dsG.sub.dsG.sub.dsG.sub.ds.sup.- mC.sub.dsA.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsG.sub.dsG.sub.k- sA.sub.ksG.sub.k 62.2 4255 4270 821 570517 .sup.mC.sub.ks.sup.mC.sub.ksT.sub.ksG.sub.ds.sup.mC.sub.dsA.sub.dsG- .sub.dsG.sub.dsG.sub.ds.sup.mC.sub.dsA.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.- sup.mC.sub.ksG.sub.ksg.sub.k 77.7 4257 4272 822 570518 .sup.mC.sub.ksG.sub.ksA.sub.ks.sup.mC.sub.dsA.sub.ds.sup.mC.sub.ds.- sup.mC.sub.dsT.sub.dsG.sub.ds.sup.mC.sub.dsA.sub.dsG.sub.dsG.sub.dsG.sub.k- s.sup.mC.sub.ksA.sub.k 79 4262 4277 823 570519 .sup.mC.sub.ksA.sub.ks.sup.mC.sub.ksG.sub.dsA.sub.ds.sup.mC.sub.dsA- .sub.ds.sup.mC.sub.ds.sup.mC.sub.dsT.sub.dsG.sub.ds.sup.mC.sub.dsA.sub.dsG- .sub.ksG.sub.ksG.sub.k 68.5 4264 4279 824 570520 A.sub.ksG.sub.ks.sup.mC.sub.ksA.sub.ds.sup.mC.sub.dsG.sub.dsA.sub.d- s.sup.mC.sub.dsA.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsT.sub.dsG.sub.ds.sup.mC- .sub.ksA.sub.ksG.sub.k 39.8 4266 4281 825 570521 G.sub.ksA.sub.ksA.sub.ksG.sub.ds.sup.mC.sub.dsA.sub.ds.sup.mC.sub.d- sG.sub.dsA.sub.ds.sup.mC.sub.dsA.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsT.sub.k- sG.sub.ks.sup.mC.sub.k 32.4 4268 4283 826 570522 .sup.mC.sub.ks.sup.mC.sub.ksA.sub.ksG.sub.dsG.sub.dsT.sub.dsA.sub.d- sG.sub.dsT.sub.dsT.sub.ds.sup.mC.sub.dsT.sub.ds.sup.mC.sub.dsA.sub.ksT.sub- .ks.sup.mC.sub.k 41 4353 4368 827 570523 .sup.mC.sub.ksA.sub.ks.sup.mC.sub.ks.sup.mC.sub.dsA.sub.dsG.sub.dsG- .sub.dsT.sub.dsA.sub.dsG.sub.dsT.sub.dsT.sub.ds.sup.mC.sub.dsT.sub.ks.sup.- mC.sub.ksA.sub.k 71.9 4355 4370 828 570524 .sup.mC.sub.ksT.sub.ks.sup.mC.sub.ksA.sub.ds.sup.mC.sub.ds.sup.mC.s- ub.dsA.sub.dsG.sub.dsG.sub.dsT.sub.dsA.sub.dsG.sub.dsT.sub.dsT.sub.ks.sup.- mC.sub.ksT.sub.k 105.9 4357 4372 829 570525 A.sub.ksG.sub.ks.sup.mC.sub.ksT.sub.ds.sup.mC.sub.dsA.sub.ds.sup.mC- .sub.ds.sup.mC.sub.dsA.sub.dsG.sub.dsG.sub.dsT.sub.dsA.sub.dsG.sub.ksT.sub- .ksT.sub.k 99.3 4359 4374 830 570526 G.sub.ksG.sub.ksA.sub.ksG.sub.ds.sup.mC.sub.dsT.sub.ds.sup.mC.sub.d- sA.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsA.sub.dsG.sub.dsG.sub.dsT.sub.ksA.sub- .ksG.sub.k 85.2 4361 4376 831 570527 .sup.mC.sub.ks.sup.mC.sub.ksG.sub.ksGdA.sub.dsG.sub.ds.sup.mC.sub.d- sT.sub.ds.sup.mC.sub.dsA.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsA.sub.dsG.sub.k- sG.sub.ksT.sub.k 82.5 4363 4378 832 570528 G.sub.ks.sup.mC.sub.ks.sup.mC.sub.ks.sup.mC.sub.dsG.sub.dsG.sub.dsA- .sub.dsG.sub.ds.sup.mC.sub.dsT.sub.ds.sup.mC.sub.dsA.sub.ds.sup.mC.sub.ds.- sup.mC.sub.ksA.sub.ksG.sub.k 60.5 4365 4380 833 570529 T.sub.ksA.sub.ksG.sub.ksA.sub.dsG.sub.ds.sup.mC.sub.dsT.sub.dsT.sub- .ds.sup.mC.sub.ds.sup.mC.sub.dsT.sub.ds.sup.mC.sub.dsT.sub.ds.sup.mC.sub.k- s.sup.mC.sub.ks.sup.mC.sub.k 35.4 4435 4450 834 570530 .sup.mC.sub.ks.sup.mC.sub.ksT.sub.ksA.sub.dsG.sub.dsA.sub.dsG.sub.d- s.sup.mC.sub.dsT.sub.dsT.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsT.sub.ds.sup.mC- .sub.ksT.sub.ks.sup.mC.sub.k 29.4 4437 4452 835 570531 A.sub.ksT.sub.ks.sup.mC.sub.ks.sup.mC.sub.dsT.sub.dsA.sub.dsG.sub.d- sA.sub.dsG.sub.ds.sup.mC.sub.dsT.sub.dsT.sub.ds.sup.mC.sub.ds.sup.mC.sub.k- sT.sub.ks.sup.mC.sub.k 30.4 4439 4454 836 570532 .sup.mC.sub.ksA.sub.ksA.sub.ksT.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsT- .sub.dsA.sub.dsG.sub.dsA.sub.dsG.sub.ds.sup.mC.sub.dsT.sub.dsT.sub.ks.sup.- mC.sub.ks.sup.mC.sub.k 30.3 4441 4456 837 570533 .sup.mC.sub.ks.sup.mC.sub.ks.sup.mC.sub.ksA.sub.dsA.sub.dsT.sub.ds.- sup.mC.sub.ds.sup.mC.sub.dsT.sub.dsA.sub.dsG.sub.dsA.sub.dsG.sub.ds.sup.mC- .sub.ksT.sub.ksT.sub.k 54.1 4443 4458 838 570534 .sup.mC.sub.ks.sup.mC.sub.ks.sup.mC.sub.ks.sup.mC.sub.ds.sup.mC.sub- .dsA.sub.dsA.sub.dsT.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsT.sub.dsA.sub.dsG.s- ub.dsA.sub.ksG.sub.ks.sup.mC.sub.k 60.1 4445 4460 839 570535 .sup.mC.sub.ksA.sub.ks.sup.mC.sub.ks.sup.mC.sub.ds.sup.mC.sub.ds.su- p.mC.sub.ds.sup.mC.sub.dsA.sub.dsA.sub.dsT.sub.ds.sup.mC.sub.ds.sup.mC.sub- .dsT.sub.dsA.sub.ksG.sub.ksA.sub.k 68.5 4447 4462 840 570536 A.sub.ksG.sub.ks.sup.mC.sub.ksA.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.- sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsA.sub.dsA.sub.dsT.sub.ds.sup.mC.s- ub.ds.sup.mC.sub.ksT.sub.ksA.sub.k 37.5 4449 4464 841 570537 G.sub.ks.sup.mC.sub.ksA.sub.ksG.sub.ds.sup.mC.sub.dsA.sub.ds.sup.mC- .sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsA.sub.dsA.s- ub.dsT.sub.ks.sup.mC.sub.ks.sup.mC.sub.k 50.9 4451 4466 842 570538 G.sub.ksG.sub.ksG.sub.ks.sup.mC.sub.dsA.sub.dsG.sub.ds.sup.mC.sub.d- sA.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.s- ub.dsA.sub.ksA.sub.ksT.sub.k 67.7 4453 4468 843 570539 T.sub.ksG.sub.ksA.sub.ks.sup.mC.sub.dsA.sub.ds.sup.mC.sub.dsA.sub.d- s.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsT.sub.ds.sup.mC.sub.dsT.sub.dsT- .sub.ksA.sub.ks.sup.mC.sub.k 55.9 4498 4513 844 570540 .sup.mC.sub.ks.sup.mC.sub.ksT.sub.ksG.sub.dsA.sub.ds.sup.mC.sub.dsA- .sub.ds.sup.mC.sub.dsA.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsT.s- ub.ds.sup.mC.sub.ksT.sub.ksT.sub.k 45.1 4500 4515 845 570541 .sup.mCkA.sub.ks.sup.mC.sub.ks.sup.mC.sub.dsT.sub.dsG.sub.dsA.sub.d- s.sup.mC.sub.dsA.sub.ds.sup.mC.sub.dsA.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.- sup.mC.sub.ksT.sub.ks.sup.mC.sub.k 30.9 4502 4517 846 570542 T.sub.ks.sup.mC.sub.ks.sup.mC.sub.ksA.sub.ds.sup.mC.sub.ds.sup.mC.s- ub.dsT.sub.dsG.sub.dsA.sub.ds.sup.mC.sub.dsA.sub.ds.sup.mC.sub.dsA.sub.ds.- sup.mC.sub.ks.sup.mC.sub.ks.sup.mC.sub.k 35 4504 4519 847 570543 .sup.mC.sub.ksA.sub.ksT.sub.ks.sup.mC.sub.ds.sup.mC.sub.dsA.sub.ds.- sup.mC.sub.ds.sup.mC.sub.dsT.sub.dsGdA.sub.ds.sup.mC.sub.dsA.sub.ds.sup.mC- .sub.ksA.sub.ks.sup.mC.sub.k 48 4506 4521 848 570544 .sup.mC.sub.ksT.sub.ks.sup.mC.sub.ksA.sub.dsT.sub.ds.sup.mC.sub.ds.- sup.mC.sub.dsA.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsT.sub.dsG.sub.dsA.sub.ds.- sup.mC.sub.ksA.sub.ks.sup.mC.sub.k 37.1 4508 4523 849 570545 .sup.mC.sub.ks.sup.mC.sub.ks.sup.mC.sub.ksT.sub.ds.sup.mC.sub.dsA.s- ub.dsTd.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsA.sub.ds.sup.mC.sub.ds.sup.mC.su- b.dsT.sub.dsG.sub.ksA.sub.ks.sup.mC.sub.k 46 4510 4525 850 570546 G.sub.ks.sup.mC.sub.ks.sup.mC.sub.ks.sup.mC.sub.ds.sup.mC.sub.dsT.s- ub.ds.sup.mC.sub.dsA.sub.dsT.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsA.sub.ds.su- p.mC.sub.ds.sup.mC.sub.ksT.sub.ksG.sub.k 79.2 4512 4527 851 570547 A.sub.ksG.sub.ksG.sub.ks.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.- sup.mC.sub.dsT.sub.ds.sup.mC.sub.dsA.sub.dsT.sub.ds.sup.mC.sub.ds.sup.mC.s- ub.dsA.sub.ks.sup.mC.sub.ks.sup.mC.sub.k 40.7 4514 4529 852 570548 G.sub.ksA.sub.ksA.sub.ksG.sub.dsG.sub.ds.sup.mC.sub.ds.sup.mC.sub.d- s.sup.mC.sub.ds.sup.mC.sub.dsT.sub.ds.sup.mC.sub.dsA.sub.dsT.sub.ds.sup.mC- .sub.ks.sup.mC.sub.ksA.sub.k 35.9 4516 4531 853 570549 A.sub.ksG.sub.ksG.sub.ksT.sub.dsA.sub.dsA.sub.dsGdA.sub.dsG.sub.dsA- .sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.ks.sup.mC.sub- .ks.sup.mC.sub.k 18.8 4613 4628 854 570550 .sup.mC.sub.ks.sup.mC.sub.ksA.sub.ksG.sub.dsG.sub.dsT.sub.dsA.sub.d- sA.sub.dsG.sub.dsA.sub.dsG.sub.dsA.sub.ds.sup.mC.sub.ds.sup.mC.sub.ks.sup.- mC.sub.ks.sup.mC.sub.k 16.2 4615 4630 855

570551 T.sub.ksT.sub.ks.sup.mC.sub.ks.sup.mC.sub.dsA.sub.dsG.sub.dsG.sub.d- sT.sub.dsA.sub.dsA.sub.dsG.sub.dsA.sub.dsG.sub.dsA.sub.ks.sup.mC.sub.ks.su- p.mC.sub.k 38.9 4617 4632 856 570552 .sup.mC.sub.ks.sup.mC.sub.ksA.sub.ksT.sub.dsT.sub.ds.sup.mC.sub.ds.- sup.mC.sub.dsA.sub.dsG.sub.dsG.sub.dsT.sub.dsA.sub.dsA.sub.dsG.sub.ksA.sub- .ksG.sub.k 28.6 4620 4635 857 570553 T.sub.ks.sup.mC.sub.ks.sup.mC.sub.ks.sup.mC.sub.dsA.sub.dsT.sub.dsT- .sub.ds.sup.mC.sub.ds.sup.mC.sub.dsA.sub.dsG.sub.dsG.sub.dsT.sub.dsA.sub.k- sA.sub.ksG.sub.k 42.6 4622 4637 858 570554 T.sub.ksA.sub.ksT.sub.ks.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsA- .sub.dsT.sub.dsT.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsA.sub.dsG.sub.dsG.sub.k- sT.sub.ksA.sub.k 31.8 4624 4639 859 570555 .sup.mC.sub.ks.sup.mC.sub.ksT.sub.ksA.sub.dsT.sub.ds.sup.mC.sub.ds.- sup.mC.sub.ds.sup.mC.sub.dsA.sub.dsT.sub.dsT.sub.ds.sup.mC.sub.ds.sup.mC.s- ub.dsA.sub.ksG.sub.ksG.sub.k 62 4626 4641 860 570556 G.sub.ksA.sub.ks.sup.mC.sub.ks.sup.mC.sub.dsT.sub.dsA.sub.dsT.sub.d- s.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsA.sub.dsT.sub.dsT.sub.ds.sup.mC- .sub.ks.sup.mC.sub.ksA.sub.k 20 4628 4643 861 570557 A.sub.ksA.sub.ksG.sub.ksA.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsT.sub.d- sA.sub.dsT.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsA.sub.dsT.sub.k- sT.sub.ks.sup.mC.sub.k 29.8 4630 4645 862 570558 T.sub.ksG.sub.ksAkA.sub.dsG.sub.dsA.sub.ds.sup.mC.sub.ds.sup.mC.sub- .dsT.sub.dsA.sub.dsT.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.ksA.sub- .ksT.sub.k 45.5 4632 4647 863 570559 T.sub.ksG.sub.ksG.sub.ks.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.- sup.mC.sub.dsG.sub.dsT.sub.dsT.sub.dsA.sub.dsG.sub.dsA.sub.dsA.sub.ksT.sub- .ksT.sub.k 72.7 4650 4665 864 570560 A.sub.ksG.sub.ksT.sub.ksG.sub.dsG.sub.ds.sup.mC.sub.ds.sup.mC.sub.d- s.sup.mC.sub.ds.sup.mC.sub.dsG.sub.dsT.sub.dsT.sub.dsA.sub.dsG.sub.ksA.sub- .ksA.sub.k 33.7 4652 4667 865 570561 G.sub.ks.sup.mC.sub.ksA.sub.ksG.sub.dsT.sub.dsG.sub.dsG.sub.ds.sup.- mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsG.sub.dsT.sub.dsT.sub.k- sA.sub.ksG.sub.k 17.5 4654 4669 866 570562 A.sub.ksG.sub.ksG.sub.ks.sup.mC.sub.dsA.sub.dsG.sub.dsT.sub.dsG.sub- .dsG.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsG.sub.k- sT.sub.ksT.sub.k 27.9 4656 4671 867 570563 .sup.mC.sub.ksT.sub.ksA.sub.ksG.sub.dsG.sub.ds.sup.mC.sub.dsA.sub.d- sG.sub.dsT.sub.dsG.sub.dsG.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.k- s.sup.mC.sub.ksG.sub.k 31.3 4658 4673 868 570564 .sup.mC.sub.ks.sup.mC.sub.ks.sup.mC.sub.ksT.sub.dsA.sub.dsG.sub.dsG- .sub.ds.sup.mC.sub.dsA.sub.dsG.sub.dsT.sub.dsG.sub.dsG.sub.ds.sup.mC.sub.k- s.sup.mC.sub.ks.sup.mC.sub.k 23.8 4660 4675 869 570565 A.sub.ksG.sub.ksG.sub.ksT.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC- .sub.dsA.sub.dsG.sub.dsA.sub.ds.sup.mC.sub.dsA.sub.ds.sup.mC.sub.dsT.sub.k- s.sup.mC.sub.ks.sup.mC.sub.k 17.2 4678 4693 870 570566 A.sub.ksT.sub.ksA.sub.ksG.sub.dsG.sub.dsT.sub.ds.sup.mC.sub.ds.sup.- mC.sub.ds.sup.mC.sub.dsA.sub.dsG.sub.dsA.sub.ds.sup.mC.sub.dsA.sub.ks.sup.- mC.sub.ksT.sub.k 33.1 4680 4695 871 570567 G.sub.ksA.sub.ksA.sub.ksT.sub.dsA.sub.dsG.sub.dsG.sub.dsT.sub.ds.su- p.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsA.sub.dsG.sub.dsA.sub.ks.sup.mC.sub- .ksA.sub.k 51.8 4682 4697 872 570568 G.sub.ksA.sub.ksG.sub.ksA.sub.dsA.sub.dsT.sub.dsA.sub.dsG.sub.dsG.s- ub.dsT.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsA.sub.ksG.sub.ksA.s- ub.k 20.3 4684 4699 873 570569 .sup.mC.sub.ksA.sub.ksG.sub.ksA.sub.dsG.sub.dsA.sub.dsA.sub.dsT.sub- .dsA.sub.dsG.sub.dsG.sub.dsT.sub.ds.sup.mC.sub.ds.sup.mC.sub.ks.sup.mC.sub- .ksA.sub.k 19 4686 4701 874

TABLE-US-00018 TABLE 18 Inhibition of human DMPK RNA transcript in HepG2 cells targeting SEQ ID NO: 2 Start Site Stop Site ISIS % Target on Seq on Seq Seq ID No. Sequence Expression ID: 2 ID: 2 No. UTC N/A 100 N/A N/A 445569 .sup.mC.sub.esG.sub.esG.sub.esA.sub.esG.sub.es.sup.mC.sub.dsG.sub.d- sG.sub.dsT.sub.dsT.sub.dsG.sub.dsT.sub.dsG.sub.dsA.sub.dsA.sub.ds.sup.mC.s- ub.esT.sub.esG.sub.esG.sub.es.sup.mC.sub.e 33.8 13226 13245 24 486178 A.sub.ks.sup.mC.sub.ksA.sub.ksA.sub.dsT.sub.dsA.sub.dsA.sub.dsA.sub- .dsT.sub.dsA.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsG.sub.dsA.sub.ksG.sub.ksG.s- ub.k 24.4 13836 13851 23 570647 G.sub.ks.sup.mC.sub.ksT.sub.ksT.sub.dsG.sub.dsG.sub.dsG.sub.ds.sup.- mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsA.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.- sup.mC.sub.ks.sup.mC.sub.ksT.sub.k 60.6 5718 5733 645 570648 A.sub.ksG.sub.ksG.sub.ks.sup.mC.sub.dsT.sub.dsT.sub.dsG.sub.dsG.sub- .dsG.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsA.sub.ds.sup.mC.sub.k- s.sup.mC.sub.ks.sup.mC.sub.k 82 5720 5735 646 570649 .sup.mC.sub.ksG.sub.ksA.sub.ksG.sub.dsG.sub.ds.sup.mC.sub.dsT.sub.d- sT.sub.dsG.sub.dsG.sub.dsG.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.k- sA.sub.ks.sup.mC.sub.k 133.4 5722 5737 647 570650 A.sub.ksG.sub.ks.sup.mC.sub.ksG.sub.dsA.sub.dsG.sub.dsG.sub.ds.sup.- mC.sub.dsT.sub.dsT.sub.dsG.sub.dsG.sub.dsG.sub.ds.sup.mC.sub.ks.sup.mC.sub- .ks.sup.mC.sub.k 54.1 5724 5739 648 570651 A.sub.ksG.sub.ksA.sub.ksG.sub.ds.sup.mC.sub.dsG.sub.dsA.sub.dsG.sub- .dsG.sub.ds.sup.mC.sub.dsT.sub.dsT.sub.dsG.sub.dsG.sub.ksG.sub.ks.sup.mC.s- ub.k 88.5 5726 5741 649 570652 G.sub.ks.sup.mC.sub.ksA.sub.ksG.sub.dsA.sub.dsG.sub.ds.sup.mC.sub.d- sG.sub.dsA.sub.dsG.sub.dsG.sub.ds.sup.mC.sub.dsT.sub.dsT.sub.ksG.sub.ksG.s- ub.k 162.9 5728 5743 650 570653 G.sub.ksA.sub.ksG.sub.ks.sup.mC.sub.dsA.sub.dsG.sub.dsA.sub.dsG.sub- .ds.sup.mC.sub.dsG.sub.dsA.sub.dsG.sub.dsG.sub.ds.sup.mC.sub.ksT.sub.ksT.s- ub.k 130 5730 5745 651 570654 A.sub.ksA.sub.ksA.sub.ksG.sub.dsG.sub.dsA.sub.dsG.sub.ds.sup.mC.sub- .dsA.sub.dsG.sub.dsA.sub.dsG.sub.ds.sup.mC.sub.dsG.sub.ksA.sub.ksG.sub.k 66.5 5734 5749 652 570655 .sup.mC.sub.ksA.sub.ksA.sub.ksA.sub.dsA.sub.dsG.sub.dsG.sub.dsA.sub- .dsG.sub.ds.sup.mC.sub.dsA.sub.dsA.sub.dsA.sub.dsG.sub.ks.sup.mC.sub.ksG.s- ub.k 79 5736 5751 653 570656 T.sub.ksG.sub.ksG.sub.ksA.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsA.sub.d- sA.sub.dsA.sub.dsA.sub.dsG.sub.dsG.sub.dsA.sub.dsG.sub.ks.sup.mC.sub.ksA.s- ub.k 57.4 5741 5756 654 570657 .sup.mC.sub.ks.sup.mC.sub.ksT.sub.ksG.sub.dsG.sub.dsA.sub.ds.sup.mC- .sub.ds.sup.mC.sub.dsA.sub.dsA.sub.dsA.sub.dsA.sub.dsG.sub.dsG.sub.ksA.sub- .ksG.sub.k 129.2 5743 5758 655 570658 .sup.mC.sub.ksA.sub.ks.sup.mC.sub.ks.sup.mC.sub.dsT.sub.dsG.sub.dsG- .sub.dsA.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsA.sub.dsA.sub.dsA.sub.dsA.sub.k- sG.sub.ksG.sub.k 66.3 5745 5760 656 570659 .sup.mC.sub.ksG.sub.ks.sup.mC.sub.ksA.sub.ds.sup.mC.sub.ds.sup.mC.s- ub.dsT.sub.dsG.sub.dsG.sub.dsA.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsA.sub.dsA- .sub.ksA.sub.ksA.sub.k 58.7 5747 5762 657 570660 G.sub.ksA.sub.ks.sup.mC.sub.ks.sup.mC.sub.dsG.sub.ds.sup.mC.sub.dsA- .sub.ds.sup.mC.sub.ds.sup.mC.sub.dsT.sub.dsG.sub.dsG.sub.dsA.sub.ds.sup.mC- .sub.ks.sup.mC.sub.ksA.sub.k 55.4 5750 5765 658 570661 A.sub.ks.sup.mC.sub.ks.sup.mC.sub.ksT.sub.dsT.sub.dsG.sub.dsT.sub.d- sA.sub.dsG.sub.dsT.sub.dsG.sub.dsG.sub.dsA.sub.ds.sup.mC.sub.ksG.sub.ksA.s- ub.k 45.4 5951 5966 659 570662 T.sub.ks.sup.mC.sub.ksA.sub.ks.sup.mC.sub.ds.sup.mC.sub.dsT.sub.dsT- .sub.dsG.sub.dsT.sub.dsA.sub.dsG.sub.dsT.sub.dsG.sub.dsG.sub.ksA.sub.ks.su- p.mC.sub.k 63.5 5953 5968 660 570663 G.sub.ks.sup.mC.sub.ksT.sub.ks.sup.mC.sub.dsA.sub.ds.sup.mC.sub.ds.- sup.mC.sub.dsT.sub.dsT.sub.dsG.sub.dsT.sub.dsA.sub.dsG.sub.dsT.sub.ksG.sub- .ksG.sub.k 56.6 5955 5970 661 570664 G.sub.ksG.sub.ksA.sub.ksG.sub.dsA.sub.dsG.sub.dsG.sub.dsA.sub.dsG.s- ub.dsG.sub.ds.sup.mC.sub.dsG.sub.dsA.sub.dsT.sub.ksA.sub.ksG.sub.k 125.6 6015 6030 662 570665 A.sub.ksG.sub.ksG.sub.ksG.sub.dsA.sub.dsG.sub.dsA.sub.dsG.sub.dsG.s- ub.dsA.sub.dsG.sub.dsG.sub.ds.sup.mC.sub.dsG.sub.ksA.sub.ksT.sub.k 64.2 6017 6032 663 570666 .sup.mC.sub.ksT.sub.ks.sup.mC.sub.ks.sup.mC.sub.dsT.sub.dsG.sub.ds.- sup.mC.sub.dsT.sub.ds.sup.mC.sub.dsA.sub.dsG.sub.dsA.sub.dsG.sub.dsG.sub.k- sG.sub.ksA.sub.k 59 6028 6043 664 570667 G.sub.ksT.sub.ksG.sub.ks.sup.mC.sub.dsT.sub.ds.sup.mC.sub.ds.sup.mC- .sub.dsT.sub.dsG.sub.ds.sup.mC.sub.dsT.sub.ds.sup.mC.sub.dsA.sub.dsG.sub.k- sA.sub.ksG.sub.k 82.3 6031 6046 665 570668 A.sub.ksG.sub.ksG.sub.ksT.sub.dsG.sub.ds.sup.mC.sub.dsT.sub.ds.sup.- mC.sub.ds.sup.mC.sub.dsT.sub.dsG.sub.ds.sup.mC.sub.dsT.sub.ds.sup.mC.sub.k- sA.sub.ksG.sub.k 96.2 6033 6048 666 570669 A.sub.ksG.sub.ksA.sub.ksG.sub.dsG.sub.dsT.sub.dsG.sub.ds.sup.mC.sub- .dsT.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsT.sub.dsG.sub.ds.sup.mC.sub.ksT.sub- .ks.sup.mC.sub.k 26.2 6035 6050 667 570670 A.sub.ksG.sub.ksA.sub.ksG.sub.dsA.sub.dsG.sub.dsG.sub.dsT.sub.dsG.s- ub.ds.sup.mC.sub.dsT.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsT.sub.ksG.sub.ks.su- p.mC.sub.k 18.2 6037 6052 668 570671 A.sub.ks.sup.mC.sub.ks.sup.mC.sub.ks.sup.mC.sub.ds.sup.mC.sub.dsG.s- ub.ds.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.d- sG.sub.ds.sup.mC.sub.dsT.sub.ks.sup.mC.sub.ksA.sub.k 29.2 6291 6306 669 570672 .sup.mC.sub.ksT.sub.ksA.sub.ks.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.s- ub.ds.sup.mC.sub.dsG.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.sup.- mC.sub.ds.sup.mC.sub.dsG.sub.ks.sup.mC.sub.ksT.sub.k 50.3 6293 6308 670 570673 A.sub.ks.sup.mC.sub.ks.sup.mC.sub.ksT.sub.dsA.sub.ds.sup.mC.sub.ds.- sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsG.sub.ds.sup.mC.sub.ds.sup.mC.sub- .ds.sup.mC.sub.ds.sup.mC.sub.ks.sup.mC.sub.ksG.sub.k 26.8 6295 6310 671 570674 G.sub.ksT.sub.ksA.sub.ks.sup.mC.sub.ds.sup.mC.sub.dsT.sub.dsA.sub.d- s.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsG.sub.ds.sup.mC.s- ub.ds.sup.mC.sub.ks.sup.mC.sub.ks.sup.mC.sub.k 40.8 6297 6312 672 570675 A.sub.ksG.sub.ksG.sub.ksT.sub.dsA.sub.ds.sup.mC.sub.ds.sup.mC.sub.d- sT.sub.dsA.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsG- .sub.ks.sup.mC.sub.ks.sup.mC.sub.k 56.1 6299 6314 673 570676 G.sub.ksG.sub.ksG.sub.ksA.sub.dsG.sub.dsG.sub.dsT.sub.dsT.sub.ds.su- p.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsG.sub.ds.sup.mC.sub.dsA.sub.ksG.sub- .ks.sup.mC.sub.k 95 6329 6344 674 570677 G.sub.ksT.sub.ks.sup.mC.sub.ks.sup.mC.sub.dsT.sub.dsT.sub.dsA.sub.d- s.sup.mC.sub.dsT.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsA.sub.dsA.sub.ds.sup.mC- .sub.ksT.sub.ksT.sub.k 23 6360 6375 675 570678 .sup.mC.sub.ksT.sub.ksG.sub.ksT.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsT- .sub.dsT.sub.dsA.sub.ds.sup.mC.sub.dsT.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsA- .sub.ksA.sub.ks.sup.mC.sub.k 23.4 6362 6377 676 570679 .sup.mCkA.sub.ks.sup.mC.sub.ksT.sub.dsG.sub.dsT.sub.ds.sup.mC.sub.d- s.sup.mC.sub.dsT.sub.dsT.sub.dsA.sub.ds.sup.mC.sub.dsT.sub.ds.sup.mC.sub.k- s.sup.mC.sub.ksa.sub.k 7.4 6364 6379 677 570680 G.sub.ksG.sub.ks.sup.mC.sub.ksA.sub.ds.sup.mC.sub.dsT.sub.dsG.sub.d- sT.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsT.sub.dsT.sub.dsA.sub.ds.sup.mC.sub.k- sT.sub.ks.sup.mC.sub.k 20.6 6366 6381 678 570681 T.sub.ksA.sub.ksG.sub.ksG.sub.ds.sup.mC.sub.dsA.sub.ds.sup.mC.sub.d- sT.sub.dsG.sub.dsT.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsT.sub.dsT.sub.ksA.sub- .ks.sup.mC.sub.k 29 6368 6383 679 570682 G.sub.ksG.sub.ksT.sub.ksA.sub.dsG.sub.dsG.sub.ds.sup.mC.sub.dsA.sub- .ds.sup.mC.sub.dsT.sub.dsG.sub.dsT.sub.ds.sup.mC.sub.ds.sup.mC.sub.ksT.sub- .ksT.sub.k 10.5 6370 6385 680 570683 G.sub.ksT.sub.ks.sup.mC.sub.ksA.sub.ds.sup.mC.sub.dsT.sub.dsG.sub.d- s.sup.mC.sub.dsT.sub.dsG.sub.dsG.sub.dsG.sub.dsT.sub.ds.sup.mC.sub.ks.sup.- mC.sub.ksT.sub.k 23 6445 6460 681 570684 G.sub.ksG.sub.ksT.sub.ks.sup.mC.sub.dsA.sub.ds.sup.mC.sub.dsT.sub.d- sG.sub.ds.sup.mC.sub.dsT.sub.dsG.sub.dsG.sub.dsG.sub.dsT.sub.ks.sup.mC.sub- .ks.sup.mC.sub.k 22.5 6446 6461 433 570685 A.sub.ksG.sub.ksG.sub.ksT.sub.ds.sup.mC.sub.dsA.sub.ds.sup.mC.sub.d- sT.sub.dsG.sub.ds.sup.mC.sub.dsT.sub.dsG.sub.dsG.sub.dsG.sub.ksT.sub.ks.su- p.mC.sub.k 10.2 6447 6462 682 570686 .sup.mCsT.sub.ksA.sub.ksG.sub.dsG.sub.dsT.sub.ds.sup.mC.sub.dsA.sub- .ds.sup.mC.sub.dsT.sub.dsG.sub.ds.sup.mC.sub.dsT.sub.dsG.sub.ksG.sub.ksG.s- ub.k 11.1 6449 6464 683 570687 G.sub.ksT.sub.ks.sup.mC.sub.ksT.sub.dsA.sub.dsG.sub.dsG.sub.dsT.sub- .ds.sup.mC.sub.dsA.sub.ds.sup.mC.sub.dsT.sub.dsG.sub.ds.sup.mC.sub.ksT.sub- .ksG.sub.k 11.7 6451 6466 684 570688 A.sub.ksA.sub.ksG.sub.ksT.sub.ds.sup.mC.sub.dsT.sub.dsA.sub.dsG.sub- .dsG.sub.dsT.sub.ds.sup.mC.sub.dsA.sub.ds.sup.mC.sub.dsT.sub.ksG.sub.ks.su- p.mC.sub.k 14.6 6453 6468 685 570689 G.sub.ks.sup.mC.sub.ksA.sub.ks.sup.mC.sub.dsT.sub.ds.sup.mC.sub.ds.- sup.mC.sub.dsA.sub.dsT.sub.dsT.sub.dsG.sub.dsT.sub.ds.sup.mC.sub.dsT.sub.k- s.sup.mC.sub.ksA.sub.k 10.1 6530 6545 686 570690 .sup.mC.sub.ksT.sub.ksG.sub.ks.sup.mC.sub.dsA.sub.ds.sup.mC.sub.dsT- .sub.ds.sup.mC.sub.ds.sup.mC.sub.dsA.sub.dsT.sub.dsT.sub.dsG.sub.dsT.sub.k- s.sup.mC.sub.ksT.sub.k 35.4 6532 6547 687 570691 .sup.mC.sub.ks.sup.mC.sub.ks.sup.mC.sub.ksT.sub.dsG.sub.ds.sup.mC.s- ub.dsA.sub.ds.sup.mC.sub.dsT.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsA.sub.dsT.s- ub.dsT.sub.ksG.sub.ksT.sub.k 33.6 6534 6549 688 570692 .sup.mC.sub.ks.sup.mC.sub.ks.sup.mC.sub.ks.sup.mC.sub.ds.sup.mC.sub- .dsT.sub.dsG.sub.ds.sup.mC.sub.dsA.sub.ds.sup.mC.sub.dsT.sub.ds.sup.mC.sub- .ds.sup.mC.sub.dsA.sub.ksT.sub.ksT.sub.k 77.3 6536 6551 689 570693 .sup.mC.sub.ksT.sub.ksT.sub.ksG.sub.ds.sup.mC.sub.dsT.sub.dsG.sub.d- sA.sub.dsG.sub.dsT.sub.ds.sup.mC.sub.dsA.sub.dsG.sub.dsG.sub.ksA.sub.ksG.s- ub.k 18.9 6559 6574 690 570694 T.sub.ks.sup.mC.sub.ks.sup.mC.sub.ksT.sub.dsT.sub.dsG.sub.ds.sup.mC- .sub.dsT.sub.dsG.sub.dsA.sub.dsG.sub.dsT.sub.ds.sup.mC.sub.dsA.sub.ksG.sub- .ksG.sub.k 30.9 6561 6576 691 570695 .sup.mC.sub.ksT.sub.ksT.sub.ks.sup.mC.sub.ds.sup.mC.sub.dsT.sub.dsT- .sub.dsG.sub.ds.sup.mC.sub.dsT.sub.dsG.sub.dsA.sub.dsG.sub.dsT.sub.ks.sup.- mC.sub.ksA.sub.k 21 6563 6578 692 570696 A.sub.ks.sup.mC.sub.ks.sup.mC.sub.ksT.sub.dsT.sub.ds.sup.mC.sub.ds.- sup.mC.sub.dsT.sub.dsT.sub.dsG.sub.ds.sup.mC.sub.dsT.sub.dsG.sub.dsA.sub.k- sG.sub.ksT.sub.k 50.3 6565 6580 693 570697 G.sub.ksG.sub.ksA.sub.ks.sup.mC.sub.ds.sup.mC.sub.dsT.sub.dsT.sub.d- s.sup.mC.sub.ds.sup.mC.sub.dsT.sub.dsT.sub.dsG.sub.ds.sup.mC.sub.dsT.sub.k- sG.sub.ksA.sub.k 28.3 6567 6582 694 570698 .sup.mC.sub.ksA.sub.ksG.sub.ksG.sub.dsA.sub.ds.sup.mC.sub.ds.sup.mC- .sub.dsT.sub.dsT.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsT.sub.dsT.sub.dsG.sub.k- s.sup.mC.sub.ksT.sub.k 47.6 6569 6584 695 570699 A.sub.ksG.sub.ks.sup.mC.sub.ks.sup.mC.sub.ds.sup.mC.sub.dsT.sub.ds.- sup.mC.sub.ds.sup.mC.sub.dsA.sub.dsG.sub.dsG.sub.dsA.sub.ds.sup.mC.sub.ds.- sup.mC.sub.ksT.sub.ksT.sub.k 17.9 6576 6591 696 570700 T.sub.ksA.sub.ksG.sub.ks.sup.mC.sub.dsT.sub.ds.sup.mC.sub.ds.sup.mC- .sub.ds.sup.mC.sub.ds.sup.mC.sub.dsA.sub.ds.sup.mC.sub.dsT.sub.ds.sup.mC.s- ub.ds.sup.mC.sub.ksA.sub.ksG.sub.k 24.1 6594 6609 697 570701 G.sub.ksA.sub.ksT.sub.ksA.sub.dsG.sub.ds.sup.mC.sub.dsT.sub.ds.sup.- mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsA.sub.ds.sup.mC.sub.dsT- .sub.ks.sup.mC.sub.ks.sup.mC.sub.k 12.9 6596 6611 698 570702 .sup.mC.sub.ksA.sub.ksG.sub.ksA.sub.dsT.sub.dsA.sub.dsG.sub.ds.sup.- mC.sub.dsT.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsA- .sub.ks.sup.mC.sub.ksT.sub.k 24 6598 6613 699 570703 .sup.mC.sub.ksT.sub.ks.sup.mC.sub.ksA.sub.dsG.sub.dsA.sub.dsT.sub.d- sA.sub.dsG.sub.ds.sup.mC.sub.dsT.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC- .sub.ks.sup.mC.sub.ksA.sub.k 22.3 6600 6615 700 570704 A.sub.ksG.sub.ks.sup.mC.sub.ksT.sub.ds.sup.mC.sub.dsA.sub.dsG.sub.d- sA.sub.dsT.sub.dsA.sub.dsG.sub.ds.sup.mC.sub.dsT.sub.ds.sup.mC.sub.ks.sup.- mC.sub.ks.sup.mC.sub.k 31.8 6602 6617 701

570705 T.sub.ks.sup.mC.sub.ksA.sub.ksG.sub.ds.sup.mC.sub.dsT.sub.ds.sup.mC- .sub.dsA.sub.dsG.sub.dsA.sub.dsT.sub.dsA.sub.dsG.sub.ds.sup.mC.sub.ksT.sub- .ks.sup.mC.sub.k 33.9 6604 6619 702 570706 T.sub.ks.sup.mC.sub.ksT.sub.ks.sup.mC.sub.dsA.sub.dsG.sub.ds.sup.mC- .sub.dsT.sub.ds.sup.mC.sub.dsA.sub.dsG.sub.dsA.sub.dsT.sub.dsA.sub.ksG.sub- .ks.sup.mC.sub.k 28.1 6606 6621 703 570707 G.sub.ksA.sub.ksG.sub.ksT.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsT.sub.d- s.sup.mC.sub.dsT.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsT.sub.dsG.sub.ds.sup.mC- .sub.ksT.sub.ksT.sub.k 37.2 6636 6651 704 570708 G.sub.ksG.sub.ksA.sub.ksG.sub.dsG.sub.dsA.sub.dsG.sub.dsT.sub.ds.su- p.mC.sub.ds.sup.mC.sub.dsm.sub.ds.sup.mC.sub.dsT.sub.ds.sup.mC.sub.ks.sup.- mC.sub.ksT.sub.k 66.3 6640 6655 705 570709 G.sub.ksA.sub.ksG.sub.ksG.sub.dsA.sub.dsG.sub.dsG.sub.dsA.sub.dsG.s- ub.dsT.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsT.sub.ds.sup.mC.sub.ksT.sub.ks.su- p.mC.sub.k 52.7 6642 6657 706 570710 .sup.mC.sub.ksA.sub.ksA.sub.ksA.sub.dsA.sub.dsG.sub.dsG.sub.dsG.sub- .ds.sup.mC.sub.dsA.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsA.sub.k- sG.sub.ksA.sub.k 31.8 6713 6728 707 570711 A.sub.ksG.sub.ks.sup.mC.sub.ksA.sub.dsA.sub.dsA.sub.dsA.sub.dsG.sub- .dsG.sub.dsG.sub.ds.sup.mC.sub.dsks.sup.mC.sub.ds.sup.mC.sub.ks.sup.mC.sub- .ksA.sub.k 12.3 6715 6730 708 570712 G.sub.ksG.sub.ksA.sub.ksT.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC- .sub.ds.sup.mC.sub.dsA.sub.dsG.sub.dsT.sub.dsA.sub.dsT.sub.dsT.sub.ksG.sub- .ksT.sub.k 37.1 6733 6748 709 570713 .sup.mC.sub.ksT.sub.ksG.sub.ksG.sub.dsA.sub.dsT.sub.ds.sup.mC.sub.d- s.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsA.sub.dsG.sub.dsT.sub.dsA.sub.k- sT.sub.kst.sub.k 42.4 6735 6750 710 570714 T.sub.ksG.sub.ks.sup.mC.sub.ksT.sub.dsG.sub.dsG.sub.dsA.sub.dsT.sub- .ds.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsA.sub.dsG.sub.k- sT.sub.ksA.sub.k 31.4 6737 6752 711 570715 A.sub.ksT.sub.ksT.sub.ks.sup.mC.sub.dsT.sub.ds.sup.mC.sub.dsT.sub.d- sA.sub.dsG.sub.dsA.sub.ds.sup.mC.sub.dsT.sub.dsG.sub.ds.sup.mC.sub.ksA.sub- .ksA.sub.k 12.1 6789 6804 712 570716 T.sub.ksA.sub.ksA.sub.ksT.sub.dsT.sub.ds.sup.mC.sub.dsT.sub.ds.sup.- mC.sub.dsT.sub.dsA.sub.dsG.sub.dsA.sub.ds.sup.mC.sub.dsT.sub.ksG.sub.ks.su- p.mC.sub.k 9 6791 6806 713 570717 T.sub.ks.sup.mC.sub.ksT.sub.ksA.sub.dsA.sub.dsT.sub.dsT.sub.ds.sup.- mC.sub.dsT.sub.ds.sup.mC.sub.dsT.sub.dsA.sub.dsG.sub.dsA.sub.ks.sup.mC.sub- .ksT.sub.k 32.1 6793 6808 714 570718 T.sub.ks.sup.mC.sub.ksT.sub.ks.sup.mC.sub.dsT.sub.dsA.sub.dsA.sub.d- sT.sub.dsT.sub.ds.sup.mC.sub.dsT.sub.ds.sup.mC.sub.dsT.sub.dsA.sub.ksG.sub- .ksa.sub.k 71.4 6795 6810 715 570719 .sup.mC.sub.ksT.sub.ks.sup.mC.sub.ks.sup.mC.sub.dsA.sub.dsT.sub.dsA- .sub.dsA.sub.dsT.sub.dsT.sub.ds.sup.mC.sub.dsT.sub.ds.sup.mC.sub.dsT.sub.k- sA.sub.ksA.sub.k 36.9 6804 6819 716 570720 A.sub.ks.sup.mC.sub.ksT.sub.ks.sup.mC.sub.dsT.sub.ds.sup.mC.sub.ds.- sup.mC.sub.dsA.sub.dsT.sub.dsA.sub.dsA.sub.dsT.sub.dsT.sub.ds.sup.mC.sub.k- sT.sub.ks.sup.mC.sub.k 17.1 6807 6822 717 570721 A.sub.ks.sup.mC.sub.ksA.sub.ks.sup.mC.sub.dsT.sub.ds.sup.mC.sub.dsT- .sub.ds.sup.mC.sub.ds.sup.mC.sub.dsA.sub.dsTdA.sub.dsA.sub.dsT.sub.ksT.sub- .ks.sup.mC.sub.k 23.7 6809 6824 718 570722 .sup.mC.sub.ks.sup.mC.sub.ksA.sub.ks.sup.mC.sub.dsA.sub.ds.sup.mC.s- ub.dsT.sub.ds.sup.mC.sub.dsT.sub.ds.sup.mC.sub.ds.sup.mC.sub.dsA.sub.dsT.s- ub.dsA.sub.ksA.sub.ksT.sub.k 34.4 6811 6826 719 570723 T.sub.ksG.sub.ks.sup.mC.sub.ks.sup.mC.sub.dsA.sub.ds.sup.mC.sub.dsA- .sub.ds.sup.mC.sub.dsT.sub.ds.sup.mC.sub.dsT.sub.ds.sup.mC.sub.ds.sup.mC.s- ub.dsA.sub.ksT.sub.ksA.sub.k 38.7 6813 6828 720

Example 10

Dose Response Studies with Antisense Oligonucleotides Targeting Human Dystrophia Myotonica-Protein Kinase (DMPK) in HepG2 Cells

[0685] Antisense oligonucleotides targeted to a human DMPK nucleic acid were tested for their effect on human DMPK RNA transcript in vitro. Cultured HepG2 cells at a density of 20,000 cells per well were transfected using electroporation with 61.7 nM, 185.2 nM, 555.6 nM, 1666.7 nM, 5000.0 nM, and 15000.0 nM concentrations of each antisense oligonucleotide. After approximately 24 hours, RNA was isolated from the cells and DMPK RNA transcript levels were measured by quantitative real-time PCR using primer probe set RTS3164 (forward sequence AGCCTGAGCCGGGAGATG, designated herein as SEQ ID NO: 20; reverse sequence GCGTAGTTGACTGGCGAAGTT, designated herein as SEQ ID NO: 21; probe sequence AGGCCATCCGCACGGACAACCX, designated herein as SEQ ID NO: 22). Human DMPK RNA transcript levels were adjusted according to total RNA content, as measured by RIBOGREEN.RTM.. Results are presented as percent expression of human DMPK, relative to untreated control (UTC) cells. For example, if the UTC is 100 and a dose of 5000 nM of ISIS No. 445569 yields a % Expression of human DMPK of 35 then the 5000 nM dose of ISIS reduced expression of human DMPK by 65% relative to the UTC. The half maximal inhibitory concentration (IC.sub.50) of each oligonucleotide is presented in the table below and was calculated by plotting the concentrations of oligonucleotides used versus the percent inhibition of human DMPK mRNA expression achieved at each concentration, and noting the concentration of oligonucleotide at which 50% inhibition of human DMPK mRNA expression was achieved compared to the control. The results are presented in Table 19.

[0686] The tested antisense oligonucleotide sequences demonstrated dose-dependent inhibition of human DMPK mRNA levels under the conditions specified above.

TABLE-US-00019 TABLE 19 Dose response studies for with antisense oligonucleotides targeting hDMPK in HepG2 Cells % Expression of ISIS No. Dose (nM) human DMPK IC.sub.50 UTC ND 100 ND 445569 61.7 115.3 2.3 185.2 87.9 555.6 69.0 1666.7 57.2 5000.0 35.0 15000.0 22.6 512497 61.7 108.6 2 185.2 98.4 555.6 77.9 1666.7 57.2 5000.0 28.0 15000.0 12.8 486178 61.7 88.2 0.7 185.2 67.1 555.6 49.4 1666.7 32.8 5000.0 26.7 15000.0 11.8 569473 61.7 107.9 0.6 185.2 66.5 555.6 33.6 1666.7 23.5 5000.0 12.8 15000.0 9.2 570808 61.7 77.2 0.2 185.2 52.7 555.6 20.6 1666.7 8.1 5000.0 7.2 15000.0 5.4 594292 61.7 96.2 5.5 185.2 99.6 555.6 80.0 1666.7 59.0 5000.0 45.5 15000.0 42.8 594300 61.7 101.7 >15 185.2 104.3 555.6 101.6 1666.7 93.6 5000.0 74.9 15000.0 66.8 598768 61.7 95.5 1.2 185.2 83.6 555.6 70.6 1666.7 40.7 5000.0 22.2 15000.0 7.3 598769 61.7 103.9 1.9 185.2 105.3 555.6 76.1 1666.7 50.4 5000.0 29.8 15000.0 12.1 598777 61.7 96.4 0.9 185.2 69.4 555.6 41.8 1666.7 42.8 5000.0 16.4 15000.0 27.1

Example 11

Dose Response Studies with Antisense Oligonucleotides Targeting Human Dystrophia Myotonica-Protein Kinase (hDMPK) in Steinert DM1 Myoblast Cells

[0687] Antisense oligonucleotides targeted to a human DMPK nucleic acid were tested for their effect on human DMPK RNA transcript in vitro. Cultured Steinert DM1 myoblast cells at a density of 20,000 cells per well were transfected using electroporation with 61.7 nM, 185.2 nM, 555.6 nM, 1666.7 nM, 5000.0 nM, and 15000.0 nM concentrations of each antisense oligonucleotide. After approximately 24 hours, RNA was isolated from the cells and DMPK RNA transcript levels were measured by quantitative real-time PCR using primer probe set RTS3164 described above. Human DMPK RNA transcript levels were adjusted according to total RNA content, as measured by RIBOGREEN.RTM.. Results are presented as percent (%) expression of human DMPK, relative to untreated control (UTC) cells. The half maximal inhibitory concentration (IC.sub.50) of each oligonucleotide is presented in the table below and was calculated by plotting the concentrations of oligonucleotides used versus the percent inhibition of human DMPK mRNA expression achieved at each concentration, and noting the concentration of oligonucleotide at which 50% inhibition of human DMPK mRNA expression was achieved compared to the control. The results are presented in Table 20.

[0688] The tested antisense oligonucleotide sequences demonstrated dose-dependent inhibition of human DMPK mRNA levels under the conditions specified above.

TABLE-US-00020 TABLE 20 Dose response studies for with antisense oligonucleotides targeting hDMPK in Steinert DM1 Cells % Expression of ISIS No. Dose (nM) human DMPK IC.sub.50 UTC ND 100 ND 445569 61.7 58.3 0.4 185.2 56.7 555.6 58.5 1666.7 40.9 5000.0 26.0 15000.0 23.5 512497 61.7 78.1 5.1 185.2 77.5 555.6 98.8 1666.7 71.2 5000.0 51.3 15000.0 22.8 486178 61.7 78.0 0.5 185.2 61.3 555.6 43.3 1666.7 27.4 5000.0 24.6 15000.0 16.9 569473 61.7 83.3 0.6 185.2 54.8 555.6 64.5 1666.7 26.1 5000.0 19.4 15000.0 15.4 570808 61.7 103.6 0.9 185.2 77.8 555.6 46.7 1666.7 25.2 5000.0 20.8 15000.0 19.3 594292 61.7 100.1 5.6 185.2 109.7 555.6 72.6 1666.7 66.2 5000.0 39.5 15000.0 45.7 594300 61.7 96.2 5.6 185.2 87.1 555.6 70.3 1666.7 66.4 5000.0 58.1 15000.0 33.2 598768 61.7 77.0 0.7 185.2 62.9 555.6 62.0 1666.7 35.6 5000.0 24.5 15000.0 21.0 598769 61.7 70.3 0.4 185.2 49.2 555.6 55.3 1666.7 33.2 5000.0 27.1 15000.0 13.4 598777 61.7 87.7 1 185.2 61.7 555.6 57.3 1666.7 37.9 5000.0 30.0 15000.0 29.7

Example 12

Dose Response Studies with Antisense Oligonucleotides Targeting Rhesus Monkey Dystrophia Myotonica-Protein Kinase (DMPK) in Cynomolgus Monkey Primary Hepatocytes

[0689] Antisense oligonucleotides targeted to a rhesus monkey DMPK nucleic acid were tested for their effect on rhesus monkey DMPK RNA transcript in vitro. Cultured cynomolgus monkey primary hepatocytes cells at a density of 20,000 cells per well were transfected using electroporation with 61.7 nM, 185.2 nM, 555.6 nM, 1666.7 nM, 5000.0 nM, and 15000.0 nM concentrations of each antisense oligonucleotide. After approximately 24 hours, RNA was isolated from the cells and DMPK RNA transcript levels were measured by quantitative real-time PCR using primer probe set RTS3164 described above. Rhesus monkey DMPK RNA transcript levels were adjusted according to total RNA content, as measured by RIBOGREEN.RTM.. Results are presented as percent (%) expression of rhesus monkey DMPK, relative to untreated control (UTC) cells. The half maximal inhibitory concentration (IC.sub.50) of each oligonucleotide is presented in the table below and was calculated by plotting the concentrations of oligonucleotides used versus the percent inhibition of rhesus monkey DMPK mRNA expression achieved at each concentration, and noting the concentration of oligonucleotide at which 50% inhibition of rhesus monkey DMPK mRNA expression was achieved compared to the control.

[0690] The tested antisense oligonucleotide sequences demonstrated dose-dependent inhibition of rhesus monkey DMPK mRNA levels under the conditions specified above.

TABLE-US-00021 TABLE 21 Dose response studies for with antisense oligonucleotides targeting rhesus monkey DMPK in cynomolgus monkey primary hepatocytes % Expression of ISIS No. Dose (nM) human DMPK IC.sub.50 UTC ND 100 ND 445569 61.7 79.7 1.4 185.2 41.1 555.6 58.1 1666.7 33.5 5000.0 46.9 15000.0 50.0 512497 61.7 123.4 1.5 185.2 63.7 555.6 44.8 1666.7 34.1 5000.0 51.2 15000.0 23.5 486178 61.7 51.1 <.06 185.2 30.6 555.6 22.0 1666.7 23.5 5000.0 9.8 15000.0 19.2 569473 61.7 82.1 .2 185.2 39.4 555.6 17.7 1666.7 28.5 5000.0 20.0 15000.0 15.6 570808 61.7 74.6 0.1 185.2 27.6 555.6 16.4 1666.7 25.6 5000.0 8.8 15000.0 21.9 594292 61.7 93.0 >15 185.2 82.1 555.6 106.0 1666.7 91.1 5000.0 62.2 15000.0 70.4 594300 61.7 105.5 >15 185.2 91.8 555.6 114.9 1666.7 65.7 5000.0 110.2 15000.0 118.8 598768 61.7 70.3 0.4 185.2 57.8 555.6 58.5 1666.7 16.5 5000.0 24.0 15000.0 13.4 598769 61.7 76.5 1.1 185.2 65.1 555.6 64.0 1666.7 34.4 5000.0 60.9 15000.0 8.6 598777 61.7 161.4 2.1 185.2 51.7 555.6 47.5 1666.7 34.6 5000.0 27.8 15000.0 52.9

Example 13

In Vivo Antisense Inhibition of hDMPK in DMSXL Transgenic Mice

[0691] To test the effect of antisense inhibition for the treatment of myotonic dystrophy type 1 (DM1), an appropriate mouse model was required. The transgenic mouse model, DMSXL carrying the hDMPK gene with large expansions of over 1000 CTG repeats was generated (Huguet et al., PLOS Genetics, 2012, 8(11), e1003034-e1003043). These DMSXL mice express the mutant hDMPK allele and display muscle weakness phenotype similar to that seen in DM1 patients.

[0692] ISIS 486178 from Table 1 was selected and tested for antisense inhibition of hDMPK transcript in vivo. ISIS 445569 was included in the study for comparison.

Treatment

[0693] DMSXL mice were maintained on a 12-hour light/dark cycle and fed ad libitum normal Purina mouse chow Animals were acclimated for at least 7 days in the research facility before initiation of the experiment. Antisense oligonucleotides (ASOs) were prepared in PBS and sterilized by filtering through a 0.2 micron filter. ASOs were dissolved in 0.9% PBS for injection.

[0694] DMSXL mice received subcutaneous injections of ISIS 445569 at 50 mg/kg or ISIS 486178 at 25 mg/kg twice per week for 4 weeks. The control group received subcutaneous injections of PBS twice weekly for 4 weeks. Each treatment group consisted of 4 animals.

Inhibition of hDMPK mRNA Levels

[0695] Twenty four hours after the final dose, the mice were sacrificed and tissues were collected. mRNA was isolated for real-time PCR analysis of hDMPK and normalized to 18s RNA. Human primer probe set RTS3164 was used to measure mRNA levels. The results are expressed as the average percent of hDMPK mRNA levels for each treatment group, relative to PBS control.

[0696] Human primer probe set RTS3164 (forward sequence AGCCTGAGCCGGGAGATG, designated herein as SEQ ID NO: 20; reverse sequence GCGTAGTTGACTGGCGAAGTT, designated herein as SEQ ID NO: 21; probe sequence AGGCCATCCGCACGGACAACCX, designated herein as SEQ ID NO: 22).

[0697] As presented in Table 22 below, treatment with antisense oligonucleotides reduced hDMPK transcript expression. The results indicate that treatment with ISIS 445569 and 486178 resulted in reduction of hDMPK mRNA levels in DMSXL mice.

TABLE-US-00022 TABLE 22 Effect of antisense oligonucleotides on hDMPK inhibition in DMSXL mice hDMPK Dosage mRNA levels ISIS No. (mg/kg) Tissue Type (% PBS) Motif/Length PBS 0 486178 25 Tibialis 70.7 kkk-d10-kkk Anterior (16 mer) Soleus 67.3 Quadriceps 73.9 Latissiumus 71.0 grand dorsi Triceps 67.1 Diaphragm 68.9 Heart 30.8 Brain 11.8 445569 50 Tibialis 38.4 e5-d10-e5 Anterior (20 mer) Soleus 47.5 Quadriceps 41.3 Latissiumus 35.7 grand dorsi Triceps 30.5 Diaphragm 44.7 Heart 7.6 Brain 13.1

Example 14

Effect of ASO Treatment on Muscle Strength in DMSXL Mice Targeting hDMPK

Griptest

[0698] Mice were assessed for grip strength performance in wild-type (WT) and DMSXL forelimb using a commercial grip strength dynamometer as described in the literature ((Huguet et al., PLOS Genetics, 2012, 801), e1003034-e1003043).

[0699] DMSXL mice received subcutaneous injections of ISIS 486178 at 25 mg/kg or ISIS 445569 at 50 mg/kg twice per week for 4 weeks. The control DMSXL group received subcutaneous injections of PBS twice weekly for 4 weeks. Each treatment group consisted of 4 animals. The forelimb force for each treatment group and WT was measured at day 0, 30, and 60 using the griptest. The grip strength performance was determined by measuring the force difference between day 60 and day 0. Results are presented as the average forelimb force from each group.

[0700] As illustrated in Table 23, below, treatment with ASOs targeting hDMPK improved muscle strength in DMSXL mice compared to untreated control. ISIS 486178, an ASO with cEt modifications, demonstrated substantial improvement in the forelimb strength (+3.4) compared to ISIS 445569 with MOE modifications (+0.38).

TABLE-US-00023 TABLE 23 Effect of ASO treatment on muscle strength in DMSXL mice targeting hDMPK Forelimb force (g) Treatment group Day 0 Day 30 Day 60 .DELTA. = Day 60-Day 0 Untreated control 72.2 70.2 67.5 -4.6 ASO 486178 62.3 65.7 65.6 +3.4 ASO 445569 64.3 68 64.7 +0.38 Wild type (WT) 75.2 76.5 78.4 +3.2

Example 15

Effect of ASO Treatment on Muscle Fiber Distribution in DMSXL Mice Targeting hDMPK

[0701] The muscle fiber distribution in DMSXL mice targeting hDMPK in the presence and absence of ISIS 445569 and 486178 was also assessed. Both ASOs were previously described in Table 1, above.

[0702] DMSXL mice received subcutaneous injections of ISIS 486178 at 25 mg/kg or ISIS 445569 at 50 mg/kg twice per week for 4 weeks. The control DMSXL group received subcutaneous injections of PBS twice weekly for 4 weeks. Each treatment group consisted of 4 animals. The muscle fiber distribution was assessed and the results are presented Table 44, below.

[0703] As illustrated, treatment with ASOs targeting hDMPK decreased the distribution of DM1 Associated Type 2c muscle fiber in the tibialis anterior (TA) of DMSXL mice compared to untreated control. The results demonstrated that normal pattern of fiber distribution in the skeletal muscles can be restored with ASO treatment. ISIS 445569 demonstrated an improvement in the muscle fiber distribution as compared to the untreated control; however ISIS 486178, an ASO with cEt modifications, demonstrated muscle fiber distribution that was more consistent with the muscle fiber distribution found in the wild-type mice.

TABLE-US-00024 TABLE 24 Effect of ASO treatment on muscle fiber distribution in DMSXL mice targeting hDMPK Fiber Type Distribution in TA muscle Treatment group Fiber 1 Fiber 2a Fiber 2c Untreated control 4% 25% 5.90% ASO 486178 3.10% 15% 0.70% ASO 445569 4% 21% 2% Wild type (WT) 3.30% 15% 0.00%

Example 16

Dose-Dependent Antisense Inhibition of hDMPK in DMSXL Transgenic Mice

[0704] The newly designed ASOs from Table 1, above, were further evaluated in a dose-response study for antisense inhibition of hDMPK transcript in vivo. ISIS 445569 was included in the study for comparison.

Treatment

[0705] DMSXL mice were maintained on a 12-hour light/dark cycle and fed ad libitum normal Purina mouse chow Animals were acclimated for at least 7 days in the research facility before initiation of the experiment. Antisense oligonucleotides (ASOs) were prepared in PBS and sterilized by filtering through a 0.2 micron filter. ASOs were dissolved in 0.9% PBS for injection.

[0706] DMSXL mice received subcutaneous injections of PBS or ASOs from Table 1, above, targeting hDMPK. The ASO was dosed twice per week for 4 weeks at the indicated doses in Table 25, below. The control group received subcutaneous injections of PBS twice weekly for 4 weeks. Each treatment group consisted of 4 animals.

Inhibition of hDMPK mRNA Levels

[0707] Forty eight hours after the final dose, the mice were sacrificed and tissue from the tibialis anterior muscles, quadriceps muscles (left), gastrocnemius muscles, heart and diaphragm was isolated. mRNA was isolated for real-time PCR analysis of hDMPK and normalized to RIBOGREEN.RTM.. Human primer probe set RTS3164 was used to measure mRNA levels. The results summarized in Table 25, below, were independently generated from various dose-response studies. The results are presented as the average percent of hDMPK mRNA expression levels for each treatment group, relative to PBS control.

[0708] As presented, treatment with antisense oligonucleotides reduced hDMPK transcript expression in a dose-dependent manner.

TABLE-US-00025 TABLE 25 Dose-dependent inhibition of hDMPK mRNA levels in DMSXL mice ISIS hDMPK mRNA levels (% PBS) No. mg/kg/wk TA Quad (Left) Gastroc Heart Diaphragm PBS 0 100 100 100 100 100 445569 50 54.7 80.3 97.1 55.4 21.7 100 28.3 42.1 71.3 48.9 19.7 200 22.2 33.9 45.2 34.2 10.0 512497 50 23.8 48.9 52.9 44.4 35.0 100 9.7 28.7 24.8 43.8 24.2 200 11.4 22.4 16.4 42.0 15.2 486178 25 59.1 56.1 63.1 75.3 39.1 50 33.8 61.9 58.7 59.2 32.5 100 36.6 65.8 51.6 47.3 26.2 570808 25 26.3 41.1 39.8 44.9 17.3 50 12.2 13.0 36.3 18.4 8.1 100 6.1 5.4 7.9 10.2 3.0 594292 25 48.8 32.2 68.8 70.6 72.7 50 32.0 30.4 41.1 85.1 48.3 100 31.6 39.6 53.3 63.9 40.2 598768 25 16.9 27.1 27.5 56.3 26.9 50 10.2 33.6 24.1 30.8 20.2 100 6.8 22.0 25.5 22.6 13.1 598769 25 21.6 50.8 48.1 61.0 30.3 50 12.7 25.1 42.3 36.4 16.7 100 12.8 18.4 33.2 32.0 20.2 569473 25 42.0 21.8 48.9 51.8 34.8 50 41.6 16.2 47.6 55.6 23.6 100 31.9 19.2 31.9 35.6 20.5 594300 25 114.5 56.7 96.2 91.0 62.6 50 44.3 22.3 52.8 69.3 54.7 100 73.0 22.6 56.6 78.3 44.5 598777 25 49.4 28.8 76.1 97.1 58.7 50 44.8 13.6 36.5 87.4 40.8 100 31.8 10.1 22.5 86.8 33.6 TA = Tibialis Anterior; Quad = Quadriceps; Gastroc = Gastrocnemius

Example 17

Six Week In Vivo Tolerability Study in CD-1 Mice

[0709] The newly designed ASOs from Table 1, above, were further evaluated in a 6 week study to assess plasma chemistry, body/organ weights and histology. Groups of CD-1 mice were administered 100 mg/kg/wk of ISIS 445569 or ISIS 512497. Further groups of CD-1 mice were administered 50 mg/kg/wk of ISIS 486178, ISIS 570808, ISIS 594292, ISIS 598768, ISIS 598769, ISIS 569473, ISIS 594300, and ISIS 598777. After six weeks and two days after each group of mice received the last dose, the mice were sacrificed and tissues were collected for analysis. For each group of mice, analysis to measure alanine transaminase levels, aspartate aminotransferase levels, blood urea nitrogen (BUN) levels, albumin levels, total bilirubin, and creatine levels was measured. Additionally, organ weights were also measured, the results of which are presented in the tables below.

TABLE-US-00026 TABLE 26 Plasma Chemistry in CD-1 mice ALT AST BUN Albumin T. Bil Creatinine ISIS No. (U/L) (U/L) (mg/dL) (g/dL) (mg/dL) (mg/dL) PBS 31.75 60.75 32.73 2.99 0.23 0.16 486178 65.00 103.00 27.18 2.90 0.19 0.13 445569 162.75 195.25 29.70 3.38 0.26 0.14 570808 313.50 332.50 32.40 2.81 0.28 0.15 594292 58.75 133.00 28.15 2.94 0.21 0.13 598768 45.50 92.00 26.85 2.90 0.21 0.11 598769 69.25 94.25 32.73 2.89 0.18 0.13 512497 101.25 144.50 26.90 2.90 0.19 0.12 569473 75.75 137.00 28.98 3.05 0.26 0.13 594300 46.00 76.75 24.70 2.94 0.18 0.11 598777 186.50 224.25 24.68 2.97 0.30 0.11

TABLE-US-00027 TABLE 27 Body & Organ Weights in CD-1 mice ISIS No. *Kidney % BW *Liver % BW *Spleen % BW PBS 1.00 1.00 1.00 486178 1.05 1.05 1.03 445569 1.07 1.09 1.23 570808 0.94 1.27 1.43 594292 1.03 1.03 1.16 598768 1.14 1.08 0.97 598769 0.97 1.05 1.04 512497 0.99 1.17 1.38 569473 1.02 1.01 1.09 594300 1.14 1.07 1.02 598777 1.05 1.20 1.01 *Fold change over Saline control group

Example 18

Six Week In Vivo Tolerability Study in Sprague-Dawley Rats

[0710] The newly designed ASOs from Table 1, above, were further evaluated in a 6 week study to assess plasma chemistry, body/organ weights and histology. Groups of Sprague-Dawley rats were administered 100 mpk/wk of ISIS 445569 or ISIS 512497. Further groups of Groups of Sprague-Dawley rats were administered 50 mpk/wk of ISIS 486178, ISIS 570808, ISIS 594292, ISIS 598768, ISIS 598769, ISIS 569473, ISIS 594300, and ISIS 598777. After six weeks and two days after each group of mice received the last dose, the mice were sacrificed and tissues were collected for analysis. For each group of mice, analysis to measure alanine transaminase levels, aspartate aminotransferase levels, blood urea nitrogen (BUN) levels, albumin levels, total bilirubin, creatine levels, and urinary creatine levels was measured. Additionally, organ weights were also measured, the results of which are presented in the tables below.

TABLE-US-00028 TABLE 28 Plasma Chemistry & Urine Analysis in Sprague-Dawley Rats ALT AST BUN Total protein T. Bil Creatinine Urine ISIS No. (U/L) (U/L) (mg/dl) (mg/dl) (mg/dl) (mg/dl) MTP/Creatine Saline 59.25 100.35 18.05 3.47 0.158 0.30 1.09 569473 101 198.25 25.9 2.74 0.195 0.4025 4.59 512497 211 240.25 19.32 3.58 0.17 0.39 6.18 598768 78.2 103.5 20.6 3.36 0.14 0.38 3.85 598769 84.5 104.5 18.6 3.52 0.15 0.34 3.02 570808 82 141 23.8 3.08 0.21 0.4 2.71 598777 109 119.5 21.65 3.79 0.22 0.37 2.56 445569 117.5 163.2 22.45 3.86 0.18 0.47 6.4 594300 66 80.75 17.53 3.59 0.12 0.29 4.72 486178 56.8 80.75 23.3 5.28 0.08 3.0 4.5 594292 64.5 80.5 19.62 3.38 0.098 0.29 5.17

TABLE-US-00029 TABLE 29 Plasma Chemistry & Urine Analysis in Sprague-Dawley Rats Kidney Liver Spleen ISIS No (fold)* (fold)* (fold)* Saline 1 1 1 569473 1.46 1.20 0.82 512497 1.03 1.22 1.94 598768 0.92 0.92 1.49 598769 0.93 1.04 0.98 570808 1.18 0.98 2.43 598777 1.07 0.93 2.31 445569 1 1.13 3.25 594300 1.03 1.04 1.94 486178 0.87 0.89 1.45 594292 1.08 1.01 2.04 *Fold change over Saline control group

Example 19

Thirteen (13) Week In Vivo Study in Cynomolgus Monkeys

[0711] Groups of 4 cynomolgus male monkeys were administered 40 mg/kg/wk of ISIS 445569, ISIS 512497, ISIS 486178, ISIS 570808, ISIS 594292, ISIS 598768, ISIS 598769, ISIS 569473, ISIS 594300, and ISIS 598777 via subcutaneous injection. Thirteen weeks after the first dose, the animals were sacrificed and tissue analysis was performed. mRNA was isolated for real-time PCR analysis of rhesus monkey DMPK and normalized to RIBOGREEN.RTM. Primer probe set RTS3164 (described above) was used to measure mRNA levels and the results are shown in Table 30 below. Additionally, further mRNA was isolated for real-time PCR analysis of rhesus monkey DMPK and normalized to RIBOGREEN.RTM. using primer probe set RTS4447 and the results are shown in Table 31 below. RTS4447 (forward sequence AGCCTGAGCCGGGAGATG, designated herein as SEQ ID NO: 20; reverse sequence GCGTAGTTGACTGGCAAAGTT, designated herein as SEQ ID NO: 21; probe sequence AGGCCATCCGCATGGCCAACC, designated herein as SEQ ID NO: 22).

TABLE-US-00030 TABLE 30 Dose-dependent inhibition of DMPK mRNA levels in Cynomolgus Monkeys using Primer Probe Set RTS3164 hDMPK mRNA levels (% PBS) ISIS Quad No. mg/kg/wk TA (Left) Gastroc Kidney Heart Liver PBS 0 100 100 100 100 100 100 486178 40 26.1 30.8 49.3 55.3 45.8 44.9 445569 40 68.5 82.2 128.9 65.6 91.2 113.5 512497 40 60.3 58.7 66.7 61.9 74.2 68.1 598768 40 69.1 64.9 80.7 58.1 70.6 100.8 594300 40 73.6 80.2 106.0 57.9 97.5 91.6 594292 40 55.6 52.0 71.9 46.2 72.1 81.6 569473 40 44.8 31.7 61.6 44.0 58.7 28.0 598769 40 31.7 28.9 49.7 26.8 45.0 38.6 570808 40 2.5 4.4 6.4 29.7 17.5 7.2 598777 40 53.3 31.8 76.4 42.7 44.6 111.6

TABLE-US-00031 TABLE 31 Dose-dependent inhibition of DMPK mRNA levels in Cynomolgus Monkeys using Primer Probe Set RTS4447 hDMPK mRNA levels (% PBS) ISIS Quad No. mg/kg/wk TA (Left) Gastroc Kidney Heart Liver PBS 0 100.0 100.0 100.0 100.0 100.0 100.0 486178 40 26.7 29.0 32.9 57.0 49.4 58.1 445569 40 85.4 87.4 147.1 77.1 97.2 93.6 512497 40 66.4 70.4 94.2 81.9 87.6 79.5 598768 40 48.3 76.4 106.7 73.7 81.0 85.1 594300 40 100.9 113.5 219.6 96.9 131.0 118.9 594292 40 76.5 75.7 151.7 86.6 107.1 108.6 569473 40 52.6 51.7 114.2 72.9 87.2 53.7 598769 40 45.2 57.6 86.3 56.6 65.4 72.5 570808 40 6.6 8.3 14.8 60.7 27.9 35.0 598777 40 55.1 56.8 124.1 78.6 88.9 131.2

Example 20

Thirteen (13) Week In Vivo Tolerability Study in Cynomolgus Monkeys

[0712] Groups of cynomolgus male monkeys were administered 40 mg/kg of ISIS 445569, ISIS 512497, ISIS 486178, ISIS 570808, ISIS 594292, ISIS 598768, ISIS 598769, ISIS 569473, ISIS 594300, and ISIS 598777 via subcutaneous injection on days 1, 3, 5, and 7. Following administration on day 7, each monkey was administered 40 mg/kg/wk of ISIS 445569, ISIS 512497, ISIS 486178, ISIS 570808, ISIS 594292, ISIS 598768, ISIS 598769, ISIS 569473, ISIS 594300, and ISIS 598777 via subcutaneous injection.

[0713] 48 hours after each monkey received a subcutaneous dose on days 28 and 91, blood and urine samples were taken for analysis. Some of the monkeys had blood and urine taken 48 hours after the dose given on day 56. Alanine aminotransferase (ALT), aspartate aminotransferase (AST), lactate dehydrogenase (LDH), and creatine kinase (CK) were measured for each animal in a treatment group and the average values are presented in the table below. Day of Sample values with a negative represent time point before treatment began. For example, a Day of Treatment value of -7 represents a sample taken 7 days before the first dose. Thirteen weeks after the first dose, the animals were sacrificed and tissue analysis was performed.

TABLE-US-00032 TABLE 32 Plasma Chemistry & Urine Analysis in Cynomolgus Monkeys Day of ALT AST LDH CK ISIS No. Sample (U/L) (U/L) (mg/dl) (mg/dl) Saline -14 34.2 25.9 604.0 160.8 -7 38.8 27.8 861.3 249.0 30 43.0 34.4 1029.0 300.0 93 66.1 43.0 1257.3 898.8 486178 -14 37.6 40.5 670.0 236.8 -7 49.8 55.0 1039.8 380.8 30 47.0 41.2 875.4 415.0 93 59.7 43.6 960.6 809.6 594292 -14 38.9 32.0 776.3 375.8 -7 37.8 38.4 877.3 210.0 30 35.4 39.6 666.0 93.8 93 49.8 46.3 958.5 339.0 569473 -14 49.4 49.8 1185.3 365.3 -7 50.4 59.7 1609.5 261.0 30 46.7 52.5 1390.8 107.8 93 56.3 49.8 1483.3 524.5 570808 -14 47.1 46.8 896.0 448.3 -7 44.4 63.6 913.3 257.3 30 47.1 57.7 660.5 125.0 93 79.8 92.2 813.5 294.0 598768 -14 37.9 41.6 666.3 253.8 -7 41.4 53.5 754.0 231.5 30 37.2 38.9 652.3 106.3 93 45.8 41.5 721.3 238.3 598769 -14 44.2 36.1 1106.8 456.8 -7 45.7 41.5 1323.3 214.0 30 40.3 42.0 981.0 147.8 58 56.7 49.9 1101.5 552.3 93 69.0 50.3 1167.3 749.5 512497 -14 31.5 34.3 689.3 293.8 -7 39.0 45.4 1110.3 286.0 30 47.2 60.2 960.5 202.5 93 69.6 87.1 997.0 1118.5 594300 -14 42.0 34.0 935.5 459.5 -7 42.1 53.6 1020.5 272.0 30 28.0 34.6 620.8 124.5 58 42.9 48.5 883.5 169.8 93 45.7 45.7 835.5 252.3 598777 -14 45.6 37.7 707.0 558.5 -7 43.3 50.0 705.8 200.3 30 50.2 47.3 585.3 159.3 93 79.2 56.1 1029.0 785.0 445569 -14 40.2 44.2 835.8 404.0 -7 41.0 46.1 1074.3 305.5 30 45.9 61.7 994.8 283.0 58 51.6 85.1 739.0 117.8 93 99.3 97.5 1583.5 2114.0

Sequence CWU 1 SEQUENCE LISTING <160> NUMBER OF SEQ ID NOS: 874 <210> SEQ ID NO 1 <211> LENGTH: 2877 <212> TYPE: DNA <213> ORGANISM: homo sapiens <400> SEQUENCE: 1 aggggggctg gaccaagggg tggggagaag gggaggaggc ctcggccggc cgcagagaga 60 agtggccaga gaggcccagg ggacagccag ggacaggcag acatgcagcc agggctccag 120 ggcctggaca ggggctgcca ggccctgtga caggaggacc ccgagccccc ggcccgggga 180 ggggccatgg tgctgcctgt ccaacatgtc agccgaggtg cggctgaggc ggctccagca 240 gctggtgttg gacccgggct tcctggggct ggagcccctg ctcgaccttc tcctgggcgt 300 ccaccaggag ctgggcgcct ccgaactggc ccaggacaag tacgtggccg acttcttgca 360 gtgggcggag cccatcgtgg tgaggcttaa ggaggtccga ctgcagaggg acgacttcga 420 gattctgaag gtgatcggac gcggggcgtt cagcgaggta gcggtagtga agatgaagca 480 gacgggccag gtgtatgcca tgaagatcat gaacaagtgg gacatgctga agaggggcga 540 ggtgtcgtgc ttccgtgagg agagggacgt gttggtgaat ggggaccggc ggtggatcac 600 gcagctgcac ttcgccttcc aggatgagaa ctacctgtac ctggtcatgg agtattacgt 660 gggcggggac ctgctgacac tgctgagcaa gtttggggag cggattccgg ccgagatggc 720 gcgcttctac ctggcggaga ttgtcatggc catagactcg gtgcaccggc ttggctacgt 780 gcacagggac atcaaacccg acaacatcct gctggaccgc tgtggccaca tccgcctggc 840 cgacttcggc tcttgcctca agctgcgggc agatggaacg gtgcggtcgc tggtggctgt 900 gggcacccca gactacctgt cccccgagat cctgcaggct gtgggcggtg ggcctgggac 960 aggcagctac gggcccgagt gtgactggtg ggcgctgggt gtattcgcct atgaaatgtt 1020 ctatgggcag acgcccttct acgcggattc cacggcggag acctatggca agatcgtcca 1080 ctacaaggag cacctctctc tgccgctggt ggacgaaggg gtccctgagg aggctcgaga 1140 cttcattcag cggttgctgt gtcccccgga gacacggctg ggccggggtg gagcaggcga 1200 cttccggaca catcccttct tctttggcct cgactgggat ggtctccggg acagcgtgcc 1260 cccctttaca ccggatttcg aaggtgccac cgacacatgc aacttcgact tggtggagga 1320 cgggctcact gccatggaga cactgtcgga cattcgggaa ggtgcgccgc taggggtcca 1380 cctgcctttt gtgggctact cctactcctg catggccctc agggacagtg aggtcccagg 1440 ccccacaccc atggaactgg aggccgagca gctgcttgag ccacacgtgc aagcgcccag 1500 cctggagccc tcggtgtccc cacaggatga aacagctgaa gtggcagttc cagcggctgt 1560 ccctgcggca gaggctgagg ccgaggtgac gctgcgggag ctccaggaag ccctggagga 1620 ggaggtgctc acccggcaga gcctgagccg ggagatggag gccatccgca cggacaacca 1680 gaacttcgcc agtcaactac gcgaggcaga ggctcggaac cgggacctag aggcacacgt 1740 ccggcagttg caggagcgga tggagttgct gcaggcagag ggagccacag ctgtcacggg 1800 ggtccccagt ccccgggcca cggatccacc ttcccatcta gatggccccc cggccgtggc 1860 tgtgggccag tgcccgctgg tggggccagg ccccatgcac cgccgccacc tgctgctccc 1920 tgccagggtc cctaggcctg gcctatcgga ggcgctttcc ctgctcctgt tcgccgttgt 1980 tctgtctcgt gccgccgccc tgggctgcat tgggttggtg gcccacgccg gccaactcac 2040 cgcagtctgg cgccgcccag gagccgcccg cgctccctga accctagaac tgtcttcgac 2100 tccggggccc cgttggaaga ctgagtgccc ggggcacggc acagaagccg cgcccaccgc 2160 ctgccagttc acaaccgctc cgagcgtggg tctccgccca gctccagtcc tgtgatccgg 2220 gcccgccccc tagcggccgg ggagggaggg gccgggtccg cggccggcga acggggctcg 2280 aagggtcctt gtagccggga atgctgctgc tgctgctgct gctgctgctg ctgctgctgc 2340 tgctgctgct gctgctgctg ctggggggat cacagaccat ttctttcttt cggccaggct 2400 gaggccctga cgtggatggg caaactgcag gcctgggaag gcagcaagcc gggccgtccg 2460 tgttccatcc tccacgcacc cccacctatc gttggttcgc aaagtgcaaa gctttcttgt 2520 gcatgacgcc ctgctctggg gagcgtctgg cgcgatctct gcctgcttac tcgggaaatt 2580 tgcttttgcc aaacccgctt tttcggggat cccgcgcccc cctcctcact tgcgctgctc 2640 tcggagcccc agccggctcc gcccgcttcg gcggtttgga tatttattga cctcgtcctc 2700 cgactcgctg acaggctaca ggacccccaa caaccccaat ccacgttttg gatgcactga 2760 gaccccgaca ttcctcggta tttattgtct gtccccacct aggaccccca cccccgaccc 2820 tcgcgaataa aaggccctcc atctgcccaa aaaaaaaaaa aaaaaaaaaa aaaaaaa 2877 <210> SEQ ID NO 2 <211> LENGTH: 14411 <212> TYPE: DNA <213> ORGANISM: homo sapiens <400> SEQUENCE: 2 ctcccagccc agcgcctccc accccttttc atagcaggaa aagccggagc ccagggaggg 60 aacggacctg cgagtcacac aactggtgac ccacaccagc ggctggagca ggaccctctt 120 ggggagaaga gcatcctgcc cgcagccagg gcccctcatc aaagtcctcg gtgtttttta 180 aattatcaga actgcccagg accacgtttc ccaggccctg cccagctggg actcctcggt 240 ccttgcctcc tagtttctca ggcctggccc tctcaaggcc caggcacccc aggccggttg 300 gaggccccga cttccactct ggagaaccgt ccaccctgga aagaagagct cagattcctc 360 ttggctctcg gagccgcagg gagtgtgtct tcccgcgcca ccctccaccc cccgaaatgt 420 ttctgtttct aatcccagcc tgggcaggaa tgtggctccc cggccagggg ccaaggagct 480 attttggggt ctcgtttgcc cagggagggc ttggctccac cactttcctc ccccagcctt 540 tgggcagcag gtcacccctg ttcaggctct gagggtgccc cctcctggtc ctgtcctcac 600 caccccttcc ccacctcctg ggaaaaaaaa aaaaaaaaaa aaaaaaagct ggtataaagc 660 agagagcctg agggctaaat ttaactgtcc gagtcggaat ccatctctga gtcacccaag 720 aagctgccct ggcctcccgt ccccttccca ggcctcaacc cctttctccc acccagcccc 780 aacccccagc cctcaccccc tagcccccag ttctggagct tgtcgggagc aagggggtgg 840 ttgctactgg gtcactcagc ctcaattggc cctgtttcag caatgggcag gttcttcttg 900 aaattcatca cacctgtggc ttcctctgtg ctctaccttt ttattggggt gacagtgtga 960 cagctgagat tctccatgca ttccccctac tctagcactg aagggttctg aagggccctg 1020 gaaggaggga gcttgggggg ctggcttgtg aggggttaag gctgggaggc gggagggggg 1080 ctggaccaag gggtggggag aaggggagga ggcctcggcc ggccgcagag agaagtggcc 1140 agagaggccc aggggacagc cagggacagg cagacatgca gccagggctc cagggcctgg 1200 acaggggctg ccaggccctg tgacaggagg accccgagcc cccggcccgg ggaggggcca 1260 tggtgctgcc tgtccaacat gtcagccgag gtgcggctga ggcggctcca gcagctggtg 1320 ttggacccgg gcttcctggg gctggagccc ctgctcgacc ttctcctggg cgtccaccag 1380 gagctgggcg cctccgaact ggcccaggac aagtacgtgg ccgacttctt gcagtggggt 1440 gagtgcctac cctcggggct cctgcagatg gggtgggggt ggggcaggag acaggtctgg 1500 gcacagaggc ctggctgttg ggggggcagg atggcaggat gggcatgggg agatcctccc 1560 atcctggggc tcagagtgtg gacctgggcc ctggggcaac atttctctgt cctatgccac 1620 cactctggag gggcagagta aggtcagcag aggctagggt ggctgtgact cagagccatg 1680 gcttaggagt cacagcaggc taggctgcca acagcctccc atggcctctc tgcaccccgc 1740 ctcagggtca gggtcagggt catgctggga gctccctctc ctaggaccct ccccccaaaa 1800 gtgggctcta tggccctctc ccctggtttc ctgtggcctg gggcaagcca ggagggccag 1860 catggggcag ctgccagggg cgcagccgac aggcaggtgt tcggcgccag cctctccagc 1920 tgccccaaca ggtgcccagg cactgggagg gcggtgactc acgcgggccc tgtgggagaa 1980 ccagctttgc agacaggcgc caccagtgcc ccctcctctg cgatccagga gggacaactt 2040 tgggttcttc tgggtgtgtc tccttctttt gtaggttctg cacccacccc cacccccagc 2100 cccaaagtct cggttcctat gagccgtgtg ggtcagccac cattcccgcc accccgggtc 2160 cctgcgtcct ttagttctcc tggcccaggg cctccaacct tccagctgtc ccacaaaacc 2220 ccttcttgca agggctttcc agggcctggg gccagggctg gaaggaggat gcttccgctt 2280 ctgccagctg ccttgtctgc ccacctcctc cccaagccca ggactcgggc tcactggtca 2340 ctggtttctt tcattcccag caccctgccc ctctggccct catatgtctg gccctcagtg 2400 actggtgttt ggtttttggc ctgtgtgtaa caaactgtgt gtgacacttg tttcctgttt 2460 ctccgccttc ccctgcttcc tcttgtgtcc atctctttct gacccaggcc tggttccttt 2520 ccctcctcct cccatttcac agatgggaag gtggaggcca agaagggcca ggccattcag 2580 cctctggaaa aaccttctcc caacctccca cagcccctaa tgactctcct ggcctccctt 2640 tagtagagga tgaagttggg ttggcagggt aaactgagac cgggtggggt aggggtctgg 2700 cgctcccggg aggagcactc cttttgtggc ccgagctgca tctcgcggcc cctcccctgc 2760 caggcctggg gcgggggagg gggccagggt tcctgctgcc ttaaaagggc tcaatgtctt 2820 ggctctctcc tccctccccc gtcctcagcc ctggctggtt cgtccctgct ggcccactct 2880 cccggaaccc cccggaaccc ctctctttcc tccagaaccc actgtctcct ctccttccct 2940 cccctcccat acccatccct ctctccatcc tgcctccact tcttccaccc ccgggagtcc 3000 aggcctccct gtccccacag tccctgagcc acaagcctcc accccagctg gtcccccacc 3060 caggctgccc agtttaacat tcctagtcat aggaccttga cttctgagag gcctgattgt 3120 catctgtaaa taaggggtag gactaaagca ctcctcctgg aggactgaga gatgggctgg 3180 accggagcac ttgagtctgg gatatgtgac catgctacct ttgtctccct gtcctgttcc 3240 ttcccccagc cccaaatcca gggttttcca aagtgtggtt caagaaccac ctgcatctga 3300 atctagaggt actggataca accccacgtc tgggccgtta cccaggacat tctacatgag 3360 aacgtggggg tggggccctg gctgcacctg aactgtcacc tggagtcagg gtggaaggtg 3420 gaagaactgg gtcttatttc cttctcccct tgttctttag ggtctgtcct tctgcagact 3480 ccgttacccc accctaacca tcctgcacac ccttggagcc ctctgggcca atgccctgtc 3540 ccgcaaaggg cttctcaggc atctcacctc tatgggaggg catttttggc ccccagaacc 3600 ttacacggtg tttatgtggg gaagcccctg ggaagcagac agtcctaggg tgaagctgag 3660 aggcagagag aaggggagac agacagaggg tggggctttc ccccttgtct ccagtgccct 3720 ttctggtgac cctcggttct tttcccccac caccccccca gcggagccca tcgtggtgag 3780 gcttaaggag gtccgactgc agagggacga cttcgagatt ctgaaggtga tcggacgcgg 3840 ggcgttcagc gaggtaagcc gaaccgggcg ggagcctgac ttgactcgtg gtgggcgggg 3900 cataggggtt ggggcggggc cttagaaatt gatgaatgac cgagccttag aacctagggc 3960 tgggctggag gcggggcttg ggaccaatgg gcgtggtgtg gcaggtgggg cggggccacg 4020 gctgggtgca gaagcgggtg gagttgggtc tgggcgagcc cttttgtttt cccgccgtct 4080 ccactctgtc tcactatctc gacctcaggt agcggtagtg aagatgaagc agacgggcca 4140 ggtgtatgcc atgaagatca tgaacaagtg ggacatgctg aagaggggcg aggtgagggg 4200 ctgggcggac gtggggggct ttgaggatcc gcgccccgtc tccggctgca gctcctccgg 4260 gtgccctgca ggtgtcgtgc ttccgtgagg agagggacgt gttggtgaat ggggaccggc 4320 ggtggatcac gcagctgcac ttcgccttcc aggatgagaa ctacctggtg agctccgggc 4380 cggggtgact aggaagaggg acaagagccc gtgctgtcac tggacgagga ggtggggaga 4440 ggaagctcta ggattggggg tgctgcccgg aaacgtctgt gggaaagtct gtgtgcggta 4500 agagggtgtg tcaggtggat gaggggcctt ccctatctga gacggggatg gtgtccttca 4560 ctgcccgttt ctggggtgat ctgggggact cttataaaga tgtctctgtt gcggggggtc 4620 tcttacctgg aatgggatag gtcttcagga attctaacgg ggccactgcc tagggaagga 4680 gtgtctggga cctattctct gggtgttggg tggcctctgg gttctctttc ccagaacatc 4740 tcagggggag tgaatctgcc cagtgacatc ccaggaaagt ttttttgttt gtgttttttt 4800 ttgaggggcg ggggcggggg ccgcaggtgg tctctgattt ggcccggcag atctctatgg 4860 ttatctctgg gctggggctg caggtctctg cccaaggatg gggtgtctct gggaggggtt 4920 gtcccagcca tccgtgatgg atcagggcct caggggacta ccaaccaccc atgacgaacc 4980 ccttctcagt acctggtcat ggagtattac gtgggcgggg acctgctgac actgctgagc 5040 aagtttgggg agcggattcc ggccgagatg gcgcgcttct acctggcgga gattgtcatg 5100 gccatagact cggtgcaccg gcttggctac gtgcacaggt gggtgcagca tggccgaggg 5160 gatagcaagc ttgttccctg gccgggttct tggaaggtca gagcccagag aggccagggc 5220 ctggagaggg accttcttgg ttggggccca ccggggggtg cctgggagta ggggtcagaa 5280 ctgtagaagc cctacagggg cggaacccga ggaagtgggg tcccaggtgg cactgcccgg 5340 aggggcggag cctggtggga ccacagaagg gaggttcatt tatcccaccc ttctcttttc 5400 ctccgtgcag ggacatcaaa cccgacaaca tcctgctgga ccgctgtggc cacatccgcc 5460 tggccgactt cggctcttgc ctcaagctgc gggcagatgg aacggtgagc cagtgccctg 5520 gccacagagc aactggggct gctgatgagg gatggaaggc acagagtgtg ggagcgggac 5580 tggatttgga ggggaaaaga ggtggtgtga cccaggctta agtgtgcatc tgtgtggcgg 5640 agtattagac caggcagagg gaggggctaa gcatttgggg agtggttgga aggagggccc 5700 agagctggtg ggcccagagg ggtgggccca agcctcgctc tgctcctttt ggtccaggtg 5760 cggtcgctgg tggctgtggg caccccagac tacctgtccc ccgagatcct gcaggctgtg 5820 ggcggtgggc ctgggacagg cagctacggg cccgagtgtg actggtgggc gctgggtgta 5880 ttcgcctatg aaatgttcta tgggcagacg cccttctacg cggattccac ggcggagacc 5940 tatggcaaga tcgtccacta caaggtgagc acggccgcag ggagacctgg cctctcccgg 6000 taggcgctcc caggctatcg cctcctctcc ctctgagcag gagcacctct ctctgccgct 6060 ggtggacgaa ggggtccctg aggaggctcg agacttcatt cagcggttgc tgtgtccccc 6120 ggagacacgg ctgggccggg gtggagcagg cgacttccgg acacatccct tcttctttgg 6180 cctcgactgg gatggtctcc gggacagcgt gccccccttt acaccggatt tcgaaggtgc 6240 caccgacaca tgcaacttcg acttggtgga ggacgggctc actgccatgg tgagcggggg 6300 cggggtaggt acctgtggcc cctgctcggc tgcgggaacc tccccatgct ccctccataa 6360 agttggagta aggacagtgc ctaccttctg gggtcctgaa tcactcattc cccagagcac 6420 ctgctctgtg cccatctact actgaggacc cagcagtgac ctagacttac agtccagtgg 6480 gggaacacag agcagtcttc agacagtaag gccccagagt gatcagggct gagacaatgg 6540 agtgcagggg gtgggggact cctgactcag caaggaaggt cctggagggc tttctggagt 6600 ggggagctat ctgagctgag acttggaggg atgagaagca ggagaggact cctcctccct 6660 taggccgtct ctcttcaccg tgtaacaagc tgtcatggca tgcttgctcg gctctgggtg 6720 cccttttgct gaacaatact ggggatccag cacggaccag atgagctctg gtccctgccc 6780 tcatccagtt gcagtctaga gaattagaga attatggaga gtgtggcagg tgccctgaag 6840 ggaagcaaca ggatacaaga aaaaatgatg gggccaggca cggtggctca cgcctgtaac 6900 cccagcaatt tggcaggccg aagtgggtgg attgcttgag cccaggagtt cgagaccagc 6960 ctgggcaatg tggtgagacc cccgtctcta caaaaatgtt ttaaaaattg gttgggcgtg 7020 gtggcgcatg cctgtatact cagctactag ggtggccgac gtgggcttga gcccaggagg 7080 tcaaggctgc agtgagctgt gattgtgcca ctgcactcca gcctgggcaa cggagagaga 7140 ctctgtctca aaaataagat aaactgaaat taaaaaatag gctgggctgg ccgggcgtgg 7200 tggctcacgc ctgtaatctc agcactttgg gaggccgagg cgggtggatc acgaggtcag 7260 gagatcgaga ccatcttggc taacacggtg aaaccccatc tctcctaaaa atacaaaaaa 7320 ttagccaggc gtggtggcgg gcgcctgtag tcccagctac tcaggaggct gaggcaggag 7380 aatggcgtga acccgggagg cagagtttgc agtgagccga gatcgtgcca ctgcactcca 7440 gcctgggcga cagagcgaga ctctgtctca gaaaaaaaaa aaaaaaaaaa aaaaaatagg 7500 ctggaccgcg gccgggcgct gtggctcatg cctgtaatcc cagcactttg ggagtccaag 7560 gccggtgggt catgagatca ggagttttga gactaggctg gccaacacgg tgaaaccccg 7620 tctctactaa aaatacaaga aaattagctg ggtgtggtct cgggtgcctg taattccagt 7680 tactggggaa gctgaggcag gagaattgct tgaacctggg aggcagagtt tgcagtgagc 7740 caagatcatg ccactacact ccagtctggg tgacagagtg agactctgtc tcaaaaaaaa 7800 aaaaaaaaaa aagggttggg caaggtggtt cacgcctgta atcccagaac tttgggaggc 7860 tgaggcaggc agatcactgg aagtcaggag ttcaagacca gcctggccaa catggtgaaa 7920 ccctgtgtct actaaaaata caaaatttag ccaggcttgg tggcgtatgc ctgtaatgcc 7980 agctactcag gaggctgagg caggagaatc gcttgattga acctgggagg cagagtttgc 8040 agtgggctgg ggttgtgcca ctgcactcta ggctgggaga cagcaagact ccatctaaaa 8100 aaaaaaaaca gaactgggct gggcacagtg gcttatattt gtaatcccag cactttggga 8160 ggctgaggtt ggaggactgc ttgagcccag agtttgggac tacaacagct gaggtaggcg 8220 gatcacttga ggtcagaaga tggagaccag cctggccagc gtggcgaaac cccgtctcta 8280 ccaaaaatat aaaaaattag ccaggcgtgg tagagggcgc ctgtaatctc agctactcag 8340 gacgctgagg caggagaatc gcctgaacct gggaggcgga ggttgcagtg agctgagatt 8400 gcaccactgc actccagcct gggtaacaga gcgagactcc gtatcaaaga aaaagaaaaa 8460 agaaaaaatg ctggaggggc cactttagat aagccctgag ttggggctgg tttgggggga 8520 acatgtaagc caagatcaaa aagcagtgag gggcccgccc tgacgactgc tgctcacatc 8580 tgtgtgtctt gcgcaggaga cactgtcgga cattcgggaa ggtgcgccgc taggggtcca 8640 cctgcctttt gtgggctact cctactcctg catggccctc aggtaagcac tgccctggac 8700 ggcctccagg ggccacgagg ctgcttgagc ttcctgggtc ctgctccttg gcagccaatg 8760 gagttgcagg atcagtcttg gaaccttact gttttgggcc caaagactcc taagaggcca 8820 gagttggagg accttaaatt ttcagatcta tgtacttcaa aatgttagat tgaattttaa 8880 aacctcagag tcacagactg ggcttcccag aatcttgtaa ccattaactt ttacgtctgt 8940 agtacacaga gccacaggac ttcagaactt ggaaaatatg aagtttagac ttttacaatc 9000 agttgtaaaa gaatgcaaat tctttgaatc agccatataa caataaggcc atttaaaagt 9060 attaatttag gcgggccgcg gtggctcacg cctgtaatcc tagcactttg ggaggccaag 9120 gcaggtggat catgaggtca ggagatcgag accatcctgg ctaacacggt gaaaccccgt 9180 ctctactaaa aatacaaaaa aattagccgg gcatggtggc gggcgcttgc ggtcccagct 9240 acttgggagg cgaggcagga gaatggcatg aacccgggag gcggagcttg cagtgagccg 9300 agatcatgcc actgcactcc agcctgggcg acagagcaag actccgtctc aaaaaaaaaa 9360 aaaaaaaagt atttatttag gccgggtgtg gtggctcacg cctgtaattc cagtgctttg 9420 ggaggatgag gtgggtggat cacctgaggt caggagttcg agaccagcct gaccaacgtg 9480 gagaaacctc atctctacta aaaaacaaaa ttagccaggc gtggtggcat atacctgtaa 9540 tcccagctac tcaggaggct gaggcaggag aatcagaacc caggaggggg aggttgtggt 9600 gagctgagat cgtgccattg cattccagcc tgggcaacaa gagtgaaact tcatctcaaa 9660 aaaaaaaaaa aaaaagtact aatttacagg ctgggcatgg tggctcacgc ttggaatccc 9720 agcactttgg gaggctgaag tggacggatt gcttcagccc aggagttcaa gaccagcctg 9780 agcaacataa tgagaccctg tctctacaaa aaattgaaaa aatcgtgcca ggcatggtgg 9840 tctgtgcctg cagtcctagc tactcaggag tctgaagtag gagaatcact tgagcctgga 9900 gtttgaggct tcagtgagcc atgatagatt ccagcctagg caacaaagtg agacctggtc 9960 tcaacaaaag tattaattac acaaataatg cattgcttat cacaagtaaa ttagaaaata 10020 cagataagga aaaggaagtt gatatctcgt gagctcacca gatggcagtg gtccctggct 10080 cacacgtgta ctgacacatg tttaaatagt ggagaacagg tgtttttttg gtttgttttt 10140 ttccccttcc tcatgctact ttgtctaaga gaacagttgg ttttctagtc agcttttatt 10200 actggacaac attacacata ctatacctta tcattaatga actccagctt gattctgaac 10260 cgctgcgggg cctgaacggt gggtcaggat tgaacccatc ctctattaga acccaggcgc 10320 atgtccagga tagctaggtc ctgagccgtg ttcccacagg agggactgct gggttggagg 10380 ggacagccac ttcatacccc agggaggagc tgtccccttc ccacagctga gtggggtgtg 10440 ctgacctcaa gttgccatct tggggtccca tgcccagtct taggaccaca tctgtggagg 10500 tggccagagc caagcagtct ccccatcagg tcggcctccc tgtcctgagg ccctgagaag 10560 aggggtctgc agcggtcaca tgtcaaggga ggagatgagc tgaccctaga acatgggggt 10620 ctggacccca agtccctgca gaaggtttag aaagagcagc tcccaggggc ccaaggccag 10680 gagaggggca gggcttttcc taagcagagg aggggctatt ggcctacctg ggactctgtt 10740 ctcttcgctc tgctgctccc cttcctcaaa tcaggaggtc ttggaagcag ctgcccctac 10800 ccacaggcca gaagttctgg ttctccacca gagaatcagc attctgtctc cctccccact 10860 ccctcctcct ctccccaggg acagtgaggt cccaggcccc acacccatgg aactggaggc 10920 cgagcagctg cttgagccac acgtgcaagc gcccagcctg gagccctcgg tgtccccaca 10980 ggatgaaaca gtaagttggt ggaggggagg gggtccgtca gggacaattg ggagagaaaa 11040 ggtgagggct tcccgggtgg cgtgcactgt agagccctct agggacttcc tgaacagaag 11100 cagacagaaa ccacggagag acgaggttac ttcagacatg ggacggtctc tgtagttaca 11160 gtggggcatt aagtaagggt gtgtgtgttg ctggggatct gagaagtcga tctttgagct 11220 gagcgctggt gaaggagaaa caagccatgg aaggaaaggt gccaagtggt caggcgagag 11280 cctccagggc aaaggccttg ggcaggtggg aatcctgatt tgttcctgaa aggtagtttg 11340 gctgaatcat tcctgagaag gctggagagg ccagcaggaa acaaaaccca gcaaggcctt 11400 ttgtcgtgag ggcattaggg agctggaggg attttgagca gcagagggac ataggttgtg 11460 ttagtgtttg agcaccagcc ctctggtccc tgtgtagatt tagaggacca gactcaggga 11520 tggggctgag ggaggtaggg aagggagggg gcttggatca ttgcaggagc tatggggatt 11580 ccagaaatgt tgaggggacg gaggagtagg ggataaacaa ggattcctag cctggaacca 11640 gtgcccaagt cctgagtctt ccaggagcca caggcagcct taagcctggt ccccatacac 11700 aggctgaagt ggcagttcca gcggctgtcc ctgcggcaga ggctgaggcc gaggtgacgc 11760 tgcgggagct ccaggaagcc ctggaggagg aggtgctcac ccggcagagc ctgagccggg 11820 agatggaggc catccgcacg gacaaccaga acttcgccag gtcgggatcg gggccggggc 11880 cggggccggg atgcgggccg gtggcaaccc ttggcatccc ctctcgtccg gcccggacgg 11940 actcaccgtc cttacctccc cacagtcaac tacgcgaggc agaggctcgg aaccgggacc 12000 tagaggcaca cgtccggcag ttgcaggagc ggatggagtt gctgcaggca gagggagcca 12060 caggtgagtc cctcatgtgt ccccttcccc ggaggaccgg gaggaggtgg gccgtctgct 12120 ccgcggggcg tgtatagaca cctggaggag ggaagggacc cacgctgggg cacgccgcgc 12180 caccgccctc cttcgcccct ccacgcgccc tatgcctctt tcttctcctt ccagctgtca 12240 cgggggtccc cagtccccgg gccacggatc caccttccca tgtaagaccc ctctctttcc 12300 cctgcctcag acctgctgcc cattctgcag atcccctccc tggctcctgg tctccccgtc 12360 cagatatagg gctcacccta cgtctttgcg actttagagg gcagaagccc tttattcagc 12420 cccagatctc cctccgttca ggcctcacca gattccctcc gggatctccc tagataacct 12480 ccccaacctc gattcccctc gctgtctctc gccccaccgc tgagggctgg gctgggctcc 12540 gatcgggtca cctgtccctt ctctctccag ctagatggcc ccccggccgt ggctgtgggc 12600 cagtgcccgc tggtggggcc aggccccatg caccgccgcc acctgctgct ccctgccagg 12660 gtacgtccgg ctgcccacgc ccccctccgc cgtcgcgccc cgcgctccac ccgccccttg 12720 ccacccgctt agctgcgcat ttgcggggct gggcccacgg caggagggcg gatcttcggg 12780 cagccaatca acacaggccg ctaggaagca gccaatgacg agttcggacg ggattcgagg 12840 cgtgcgagtg gactaacaac agctgtaggc tgttggggcg ggggcggggc gcagggaaga 12900 gtgcgggccc acctatgggc gtaggcgggg cgagtcccag gagccaatca gaggcccatg 12960 ccgggtgttg acctcgccct ctccccgcag gtccctaggc ctggcctatc ggaggcgctt 13020 tccctgctcc tgttcgccgt tgttctgtct cgtgccgccg ccctgggctg cattgggttg 13080 gtggcccacg ccggccaact caccgcagtc tggcgccgcc caggagccgc ccgcgctccc 13140 tgaaccctag aactgtcttc gactccgggg ccccgttgga agactgagtg cccggggcac 13200 ggcacagaag ccgcgcccac cgcctgccag ttcacaaccg ctccgagcgt gggtctccgc 13260 ccagctccag tcctgtgatc cgggcccgcc ccctagcggc cggggaggga ggggccgggt 13320 ccgcggccgg cgaacggggc tcgaagggtc cttgtagccg ggaatgctgc tgctgctgct 13380 gctgctgctg ctgctgctgc tgctgctgct gctgctgctg ctgctggggg gatcacagac 13440 catttctttc tttcggccag gctgaggccc tgacgtggat gggcaaactg caggcctggg 13500 aaggcagcaa gccgggccgt ccgtgttcca tcctccacgc acccccacct atcgttggtt 13560 cgcaaagtgc aaagctttct tgtgcatgac gccctgctct ggggagcgtc tggcgcgatc 13620 tctgcctgct tactcgggaa atttgctttt gccaaacccg ctttttcggg gatcccgcgc 13680 ccccctcctc acttgcgctg ctctcggagc cccagccggc tccgcccgct tcggcggttt 13740 ggatatttat tgacctcgtc ctccgactcg ctgacaggct acaggacccc caacaacccc 13800 aatccacgtt ttggatgcac tgagaccccg acattcctcg gtatttattg tctgtcccca 13860 cctaggaccc ccacccccga ccctcgcgaa taaaaggccc tccatctgcc caaagctctg 13920 gactccacag tgtccgcggt ttgcgttgtg ggccggaggc tccgcagcgg gccaatccgg 13980 aggcgtgtgg aggcggccga aggtctggga ggagctagcg ggatgcgaag cggccgaatc 14040 agggttgggg gaggaaaagc cacggggcgg ggctttggcg tccggccaat aggagggcga 14100 gcgggccacc cggaggcacc gcccccgccc agctgtggcc cagctgtgcc accgagcgtc 14160 gagaagaggg ggctgggctg gcagcgcgcg cggccatcct ccttccactg cgcctgcgca 14220 cgccacgcgc atccgctcct gggacgcaag ctcgagaaaa gttgctgcaa actttctagc 14280 ccgttccccg cccctcctcc cggccagacc cgccccccct gcggagccgg gaattccgag 14340 gggcggagcg caggccgaga tggggaatgt gggggcctgc agaggaccct ggagacggag 14400 gcgtgcagaa g 14411 <210> SEQ ID NO 3 <211> LENGTH: 15000 <212> TYPE: DNA <213> ORGANISM: mus musculus <400> SEQUENCE: 3 cagtgtcccc actgcccaag gctggctcca tcacgtaccg ctttggctca gctggccagg 60 acacacagtt ctgcctgtgg gacctcacag aagatgtgct ctcccctcat ccgtctctgg 120 cccgtacccg cacccttccg ggcacacctg gtgccacccc accagcttct ggtagttctc 180 gggccggaga gacaggtgca ggccccctgc cccgctccct gtctcgttcc aacagtctcc 240 cacacccagc tggtggtggc aaggctggtg ggcctagtgc atcgatggag cctggcatac 300 cattcagcat tggccgcttt gccacactga ccctgcagga gcggcgggac cggggagctg 360 agaaggaaca caaacgctac catagcctgg gaaacatcag ccgcggtggc agtgggggca 420 atagcagcaa tgacaagctc agtggtcctg ccccccgaag ccgattggac ccagctaagg 480 tgctgggcac ggcactgtgc cctcggatcc atgaggtgcc actgctggag cctctcgtgt 540 gcaagaagat tgctcaggaa cgcctgaccg tgctactgtt cctggaggat tgtatcatca 600 ctgcctgcca agagggcctc atctgcacct gggcccggcc aggcaaggcg gtgagtccgc 660 acctgcccaa gcgctgaggg gcaccagttc tgtccctacc ggatgccagt tatccgtcag 720 cagaaaggtc aggtatagga gacagaatgg ggggaaccac agctaacgtc tttagagcct 780 ctgctggccc atatggctca tccttagtac ttcacactca aggcagaacc tgtgtttata 840 ggaaatctga agtgtagatg gtgaaacttt attcaggtct agggatgtga ttgagctggg 900 ggcccacttc tggcctgcct cttagacact gtttctgagc cagctgctga aggcctggat 960 gggaattagc cagggtccag gcctgcactt cctcttgctg ctgtgtggtc ctggtcattg 1020 ggtctcacag atgggctgtg cagtggctgt gctcttagtt ggtgaggtgc aggcctgtca 1080 cctggtcagg cttgagcatg tggtctcagt gtctaggacc ctactctgcc ctcagtcctt 1140 cagtcccttg ctttggaagg ctagagtcca gaagccttag aacgtcaggc agttgcagag 1200 ccactgccag gctagtaggg ctgcgggagt tgactgagtt ctcacagaca cccctctgtc 1260 tccctagttc acagacgagg agaccgaggc ccaggcaggg caagcaagtt ggcccaggtc 1320 acccagcaag tcagttgtag aggtaggaca acccctgaag ctgcaagtgg accccagttt 1380 cttttctctc cactgtcgtc ccctgtatgc ccaggacacc tggggccaca ttactgtgga 1440 agtgctactc tgggtcagtg gagacggccg agctgtttgt tcctagctag gacagcagct 1500 ttaggcctgg ggggcagatc ccagctgggg cagcagctcc aaggcctttg ggtggctcct 1560 tctccgggtt ctggcagaag cccaggtgct gtctaatcca cctttctcct cttgttctcc 1620 ccagggcatc tcctcccaac caggcagctc ccccagtggc actgtggtgt gaaatgtgga 1680 tgtcccatgt tcccggcctc ctagccataa ccctccccgc tgacctcaag aatcactgta 1740 ttaacaagac taatcatgat ggaaggactg ctccaagccc cacgctgcac acatactggg 1800 ggtcccctag gttggcccag ccatggggat gtagtgtcct gtgtggcctt ggccctgtcc 1860 tccacccact gccaagtaca atgacctgtt ctctgaaaca tcagtgttaa ccatatccct 1920 gtcccagcat gtgactgttc actcctggga gagacttagc ccacagtacc cctgggtgag 1980 agggcagggc aggggccatc cccactcctg cccaaactcc accccttgct atggtctgtg 2040 attttgaaag tgttaaatta tggaagccct gagggccctc cttgttcccc tggacctctt 2100 atttatacta aagtccttgt ttgcacagtg tttctgttcc ctggggcagg gtagggtggg 2160 ggttgcagta cttggcctcc aagctgtgct ctgaccaaag gaagcccaat cttagctgtt 2220 tccccatccc tagccccgag cagagagccc tctgaaagat gagtctcgac ccccaaagtc 2280 aagaggctga gatggccttc ctactaggtc cttggagatg tttgaaactt gttttaaaca 2340 ccaggactat ccaagcatgc tctccttggg gagaggagga tgctggaatt gactgcactc 2400 cctgcctcct ctgaacatgc ctttgcagtc tgctgcccct ggcccattta tgactggcca 2460 tctagtgcca gctggaggtc atgatttcct ccccagagaa ctggccaccc tagaaagaag 2520 ctaacttgtc gcctggcttg ctgtccaggc agctccgccc tcaaccccta aaatgtttct 2580 gtctctaatc ctagcccagg caggaatgtg gctgccccgg cctgtggcca aggagctatt 2640 ttggggttct cttttgctta aggagggcct ggatccacca cttgcctccc ccaggctggg 2700 gccagcaggt cacccctggc cctggcggct gagcaaactc tctcctgatc ttccttctac 2760 ctcctgccaa aaaatggggg ggcgggtaat acagcaggca caggggctaa atttaactgt 2820 cccaaagtcg gaatccattg ctgagtcacg aagaagctgc ccctggcctt tgcccccccc 2880 actaccccct caccccctgt tgcccaggca tcagcccttt cccccaaccc ctcccagctc 2940 tgagtctata gactggctct cctgggcact gacacctccc acctgtaact ccctgtgctc 3000 tctttatggg tgggtagagt caatgggggg gggcaaccct ggagtattac tctgtcccct 3060 gacattgggc tctgaagagt tttgaggggc cctggaagaa gggagttggg gtgttggctc 3120 aggaggggtt aaaaactggg aggcgggagg ggggctgggc caaggggtgg agaaaagagg 3180 aggaggcctt aagcatagaa ctggccagag agacccaagg gatagtcagg gacgggcaga 3240 catgcagcta gggttctggg gcctggacag gggcagccag gccctgtgac gggaagaccc 3300 cgagctccgg cccggggagg ggccatggtg ttgcctgccc aacatgtcag ccgaagtgcg 3360 gctgaggcag ctccagcagc tggtgctgga cccaggcttc ctgggactgg agcccctgct 3420 cgaccttctc ctgggcgtcc accaggagct gggtgcctct cacctagccc aggacaagta 3480 tgtggccgac ttcttgcagt ggggtgagta tggataggaa gcctggggtt gggtgcaagg 3540 cagaggtggg tctacagggc aagaatgggc tatggagggg caggagggcc tggaaagggc 3600 tttttgtaag ggagccaagc agagctcatg acctgacccc aagctcccct ggtgaggcac 3660 cagggtcagt gaggccacct atgactcagc cagtgcaggc tggggtgggc atagcctcct 3720 gctatctcag cacccacact aggacctggc agctttctct tttaggaccc ttggctcctc 3780 aaactggctt catagccctc cccagtttcc cagagtgtgg ggagggacag cgtggggcag 3840 ctgccagggt gtggcccata ggcaggtgtt tggcgtctgc ctccccagct gccctgacag 3900 gtgtccagga gctatgaggg cactgtgact cacagaggcc ctgggggaga accagcccgg 3960 cagacaggcg ccaccgagca ccctttctgt tccccaaatt aagaggaagg aacaacttca 4020 gcttctgagt gtgcccatcc ctagcactct gatcccgccc agcctttgtg ggccagattg 4080 gtcatccctc ctggcttctc atctgctttt gtggttctag ctcaagacct ctaattcctc 4140 tgctgactta aatgcccttc cccagaggtc ttctcaggcc tagtggacaa gcttggagcc 4200 ttatctgctc ctgcccaaca ttgagccaaa gctccagctt accccagctt ccttacaagt 4260 aacgacctgt tttgttgctc tgtgcctatt attaagggtc caggtcttga ttcttggctg 4320 tctgcccatg tgtgtgaccc tagtgcattc tcccctcctc ccccgtttca cagatggaaa 4380 ggttgaggcc atcggttaga ctgctaagcc tgtgaaagac tttttctcct ctccagtctt 4440 tagtgtctcc ctcaaccttt cttttgaagg atggggtttg ggctggcagg gtaaactgag 4500 aactggggtg ggggcagggg gtctgaccct ctgggaagga gcagtccttt tgtggcctga 4560 gcagcatcct gtgggcccct cccctgccag gcctgggcgg gggagggggc ctgggttccc 4620 gctgccttaa aagggctcaa cgccttggct ctctcctcct ccccaccccc cagccttggc 4680 cctagctgta tcttccccgg ctgcccactt tcccaaaccc ctttcttctc tgtgacccca 4740 tctccccgct tccccacacg tccctcctcc atccttactc cccggcctta gaacttccct 4800 aagggagatc tgacctccct ctgcccaccc cgcaccccca gtcgccagcc tcagacctag 4860 ctgctctccc ctctggctga accaccctag cacaggacct tataccctgg agctttggtt 4920 ataagaagac tctccttcac cctttggaaa ccaagaaagc ccttccaaca gtgtccagga 4980 tgctggaggg cagtgaccct cccccacttc ttcttcgtgc tggctgtgct gacacagctc 5040 cagttcgagg ttgtggcccg agacattaag tgagagcccc gggtgacctg acttagcacc 5100 ctgatcatca catgggagtg aaaggcctga tgcgccagct tctcccactg cctccctttc 5160 tgccctgcaa ccctgtggaa acaggcagtt ctgggtccca caaacatcac agaggttttg 5220 aaagcagaat cctaaagccg atttaagggg cagaaggaag gaggctataa agtcactacc 5280 cttaccgcta gtgttctgat gacccttggt tcttcttccc ccacccccgc ccagtggagc 5340 ccattgcagc aaggcttaag gaggtccgac tgcagaggga tgattttgag attttgaagg 5400 tgatcgggcg tggggcgttc agcgaggtga gtcttcagtg gcctgggaat ggaactttac 5460 ttgatgtggg tggggcataa cagctggggc agagccttaa aaattgatga atgagcttga 5520 atttaaggct ggaggggtgg gggcggagct tgtggtcagt gggcggtgtg cacgtgaggg 5580 cggggctaag gttgggtgga gataagggtg gagtcctgtc tgggtgagcc ttgctggttt 5640 tccctgccac ctcttgctgt catctcggtt ccgtatttag gtagcggtgg tgaagatgaa 5700 acagacgggc caagtgtatg ccatgaagat tatgaataag tgggacatgc tgaagagagg 5760 cgaggtgagg gccagggatt agggcagcgc cctcatctct ccaactcacc tcctgtagct 5820 tctctcctac ctcacaggtg tcgtgcttcc gggaagaaag ggatgtatta gtgaaagggg 5880 accggcgctg gatcacacag ctgcactttg ccttccagga tgagaactac ctggtaagct 5940 ccgggttcag gtgactagga aagagtgaca gttacatcgc cccaagtcaa gaaggctgga 6000 gaagggagaa gctgctgtag atcggggggg tgggggtggg ggggacacac acaggggatg 6060 ggggacgggg gtaggattgt gtctcaagta taggagagac cttccttgag acaggagtga 6120 tatctggttt ggcctttgga tggggcgctc tctcactgtg cgggggtcct ctgtgcttgg 6180 gaacggggtg tctttgggag tcttgggggc taccaaaccc ctgtgacaca cccgctccca 6240 gtacctggtc atggaatact acgtgggcgg ggacctgcta acgctgctga gcaagtttgg 6300 ggagcggatc cccgccgaga tggctcgctt ctacctggcc gagattgtca tggccataga 6360 ctccgtgcac cggctgggct acgtgcacag gtgggcgtgg cggggccctt ggagggttag 6420 cagaatttgt gtgggaagga agggtacctg aaggtcagat cccattgggg acagaatcgg 6480 ggtctagaat tgtagaatcc tgggtggggt ggaagtggat cgagctgacg ggccctaaga 6540 gggaaggttt tcaagaaagc acactttccc tcttctctct atgcacaggg acatcaaacc 6600 agataacatt ctgctggacc gatgtgggca cattcgcctg gcagacttcg gctcctgcct 6660 caaactgcag cctgatggaa tggtaagaag agcctggcga aactctcctc attggtgaag 6720 gaccggatta gggggcgggg ctgggttgag gagcaggagg ggagcttggt ctgggatgtc 6780 ctgcgcacca tatttggaca gtcaagggaa aggttttaag cattcaggtc tgattggcac 6840 aggtgaggtc gctggtggct gtgggcaccc cggactacct gtctcctgag attctgcagg 6900 ccgttggtgg agggcctggg gcaggcagct acgggccaga gtgtgactgg tgggcactgg 6960 gcgtgttcgc ctatgagatg ttctatgggc agaccccctt ctacgcggac tccacagccg 7020 agacatatgc caagattgtg cactacaggg tgagcacaag caccatgcag gggggctgac 7080 ttagtggctt gtgctcccag actgtctttt ttaaaagata tttatttata tgtgtgtgtt 7140 ttctgtgtat gtatatctgt gcactgagta ggtgtgcgaa ggtcagaggg catgggatcc 7200 cctggaactg gagtcacaga ctattgtgtg ctgccatgct gagtgctggg aaacagaacc 7260 ttgatcactc tgcaagagca gccagtgcac tgaaacgaca gagccagctc tgcagcccag 7320 ggctaactgt tgcttttctt tctaaatagg aacacttgtc gctgccgctg gcagacacag 7380 ttgtccccga ggaagctcag gacctcattc gtgggctgct gtgtcctgct gagataaggc 7440 taggtcgagg tggggcaggt gatttccaga aacatccttt cttctttggc cttgattggg 7500 agggtctccg agacagtgta ccccccttta caccagactt cgagggtgcc acggacacat 7560 gcaatttcga tgtggtggag gaccggctca ctgccatggt gagcgggggc ggggtacgta 7620 cctgcagttc ctgatccgtt gaggggactt ccctagcctc ttccataaaa ttggggtgat 7680 tggccaggtg tggtggtgca tacctttaat cgtagaactt cataggcaga ggcaggtggc 7740 tctctggtaa atcaaggcca tcttggtcta catagtgact tctaggccag tcaggagtga 7800 gatcctccct tgaaaaataa aaaagggggt gttgaccttc ctgggtccca aattattatc 7860 ctagagcact gctatgtatc cactcaggta tgaggacaca caggtgacca gtcccaaaga 7920 cagtgagtga ggcctcactc ttggcagtac taaaattgat tgtagggggc tgggctcttg 7980 acccagcctg gaaagtgctg gagggcttcc tggaggagga gactagctga gcccagaagg 8040 atgcaggaga tcctttctcg ggtgagtgct ctcagcattt taacaagctc taggccctgc 8100 agagagaagt ctggtgtggg cagagcccca atagaaagca acaagataga agagaaaatg 8160 gtggagtttg ttagtggggg cagttatgcc gtgaacatag aggggcgaag ggccatctcg 8220 gataactgct agccacaaga gccctgtctg tcttcctagg agacgctgtc agacatgcag 8280 gaagacatgc cccttggggt gcgcctgccc ttcgtgggct actcctactg ctgcatggcc 8340 ttcaggtgag cacgactgcc ccctgctggg gcctgtgtgc aggcccacca cagccactca 8400 attgaaggct cagtcttcaa accaagtatt cctaggagct gtctaagtta ggctttctgc 8460 tgctgcgatg aaccctgact aaaagcaagc tggggaggaa aaggcttatc gggcttacgt 8520 ttccacatgg gagcccatca ctgaaggaag ccaggacagg aactcacagc ggggcaggaa 8580 cgtggagctg atgcagaggc aatggagggg agctgcttac tgacttgatc cttatgtctt 8640 cctcagcctg tttccttgta gagcccagga ccaccaggcc agtgagggct ccactcacaa 8700 tgggctgagc tctcatctat gatcactagt tatgaaaatg cccgataggc ttgcctgcag 8760 cttcagtttt tgaggcactt tccttccttc cttccttcct tccttccttc cttccttcct 8820 ttctttcttt ctttctttct ttctttcttt ctttctttct ttctttcttt ctttctttct 8880 tagtctttta gagacagggt ctttctatgt agctctggct gtcttggaat tcattctgta 8940 gaccaggctg gtcttattta tttattttat gtatgtgagt ccactatcac tgtcctcaga 9000 cacaccagaa gagggcatca gatcccatta cagatggctg tgagccacca tgtggttgct 9060 gggaattgaa ctcaggacct ctggaagagc agccagtgct cctgccctgt agaggcattt 9120 tcttcatgaa ggctgtctcc tctctgatga cttgatgact ctagcttgtt gtgtcaagtg 9180 gacataagac taggaaagca gctacacatg cactttgttt atttttgttt tgctttttga 9240 gactgggtct ctccatctca tagctctggc catcctgcct ggtgacattc cagtccagtt 9300 gtataaccta agaatctgag actcagcctt gcagaatcct gctattaacg ggtctaggac 9360 actccataga atccaggatc ttagaaaaac aaacctgaag tgtgacagtt tattttaaga 9420 acacaattgg agcacataac aataatacaa cttttcagtt ttaaaaagtt ttctgtcttg 9480 ttttttgagg caggagctcc ttaatatagt ctaagccgcc ctgcgagtgc tgtgattgat 9540 gggcatgtac caccatgcct agtcaataaa gcctttaaaa agcatccgtt atgctggctg 9600 tggtgccaca aacctgtaat cccagcactt agaaggtaga ggcaagatta tcagaaattc 9660 aaggccatcc tgggctatac agtaatctaa ggctagcctg gtctacaaga gactctgtct 9720 aaagaaacaa aagataaata gcacccacta ttgctaggca atataaccct ataaccccac 9780 cattgaggag gctgaggctg gagcatcact gcaaatttga ggccaggatg gtcaacaaat 9840 aagtcccaga gctggcatag aggaactctg tctcaacaat aaagagaact tatctagcat 9900 ttatgagggt aaataaaaat ttaccattgc cacaaaaaat gtaaatgaag agactgcttt 9960 taggagtgaa ctgggaagca gggaacactt agaggatgct cactcacaca ggtatccacc 10020 atcaggcatg cctcaggcct gcacagggaa ggacaacttg tttcatgatt tgcaagcagc 10080 atcccatgct ccttagagcg ggttgggccc agcccaccct ctgtggagtt atcgctcagc 10140 caggcagcaa ggcagccaag gtgctgaggc cctggcagtc tgctctcttc tctgctctga 10200 acctccttta gctttagcct aggagcctgg cctggtgccc acaggctagg gagtccctag 10260 cctcttcctc ttctcagaga caatcaggtc ccggacccca cccctatgga actagaggcc 10320 ctgcagttgc ctgtgtcaga cttgcaaggg cttgacttgc agcccccagt gtccccaccg 10380 gatcaagtgg tgagtagact gagaggtggg caaagcttcc tgggtgggtg tacctgcagt 10440 gccaactgcc aggctgttaa ttcagtagga cactgtcccc aactggccca actgcacatc 10500 ctgtagtcag gaggcacagg cagaaaaatc ccaaattcaa ggcttgctcc cgttatgtaa 10560 tgagatcctg tcttggagta aaaaacaaag aagagaacta gggatagctc agaggtagat 10620 gctctcctgg catgggggtg gggtcagaaa gcaacaccaa ccggggcctg ggagggaggg 10680 actgccaacc acctggagga gtctggggta gacttggtga acaaagttca gaggccatca 10740 ggtgggatgc tggtttctta aaagccacag ataggtgggt agcattggaa agaggagtgg 10800 ggggttgcag aaagtgacaa gacacaaact ggggaggcct aagggtaaag ccagggttgt 10860 ctgaagcact gtggagctgg gaggaacacg ctaaacttct gacttcagcc cttcagttcc 10920 cctgttgact acactgtccc cagggaccca gggatgggga gaggtggacg ggggagggaa 10980 gtacgggact gatccagctc caggtcccaa ctctgatccc caccgacagg ctgaagaggc 11040 tgacctagtg gctgtccctg cccctgtggc tgaggcagag accacggtaa cgctgcagca 11100 gctccaggaa gccctggaag aagaggttct cacccggcag agcctgagcc gcgagctgga 11160 ggccatccgg accgccaacc agaacttctc caggtcaggg tcacagtgct ggggtgaggg 11220 gagaggagag cagcaaccct cgcagtctcc tcaccgatag gtcggctcac tcccctatct 11280 ttcccagcca actacaggag gccgaggtcc gaaaccgaga cctggaggcg catgttcggc 11340 agctacagga acggatggag atgctgcagg ccccaggagc cgcaggcgag tccctcacct 11400 gcttccagcc aagggggcac tgggtggaga tggggggcat gttgggtgtg tgaaccctcg 11460 gggcagggga ggagtccagg ctggggcacc gcagccgcgc cactgccttt ctcctccatc 11520 ctccacactc catacacctc tctcttctcc ttccagccat cacgggggtc cccagtcccc 11580 gggccacgga tccaccttcc catgtaagac ccctctctcc cctccccgat ccccatctta 11640 gatatgctac ccacagccct tctcccgtcc acgtttaggg tccattctcc ttgggggttc 11700 cagaagaaag ctgcccttca ctcatccatt cagcatgcac tatctaccag ctctccctcg 11760 tttcaggctt ctcgccaaat cctccccaag ggaactccct atactcccgt tctggcctcg 11820 actagattcc cgcactgcct ctcgccctgc tgctgggctc cgatcgggtc acctgtccct 11880 tctctctcca gctagatggc cccccggccg tggctgtggg ccagtgcccg ctggtggggc 11940 caggccccat gcaccgccgt cacctgctgc tccctgccag ggtatgtccc acgtccgccc 12000 accacgggcc tctgcctagc tctgcccact gagtgtcacc actgcttgct gtgcctctgt 12060 ggagctcggc ccaccgcagg gagggggggt attcgggcgg ccaatcaaca caggctgctg 12120 ctaagtagcc aatgacgagt tccaacagga gctctttctt gcgagcagac caactttagc 12180 tgcgggctgt ggggaccaga gatgcgctca gaggcccatc tatgggtata ggctgggcgg 12240 ctcccaggag ccagtgggcc cctgtagcct agtgctaatc caaccttctc tcctgcagat 12300 ccctaggcct ggcctatccg aggcgcgttg cctgctcctg ttcgccgctg ctctggctgc 12360 tgccgccaca ctgggctgca ctgggttggt ggcctatacc ggcggtctca ccccagtctg 12420 gtgtttcccg ggagccacct tcgccccctg aaccctaaga ctccaagcca tctttcattt 12480 aggcctccta ggaaggtcga gcgaccaggg agcgacccaa agcgtctctg tgcccatcgc 12540 gccccccccc cccccccacc gctccgctcc acacttctgt gagcctgggt ccccacccag 12600 ctccgctcct gtgatccagg cctgccacct ggcggccggg gagggaggaa cagggctcgt 12660 gcccagcacc cctggttcct gcagagctgg tagccaccgc tgctgcagca gctgggcatt 12720 cgccgacctt gctttactca gccccgacgt ggatgggcaa actgctcagc tcatccgatt 12780 tcactttttc actctcccag ccatcagtta caagccataa gcatgagccc cctatttcca 12840 gggacatccc attcccatag tgatggatca gcaagacctc tgccagcaca cacggagtct 12900 ttggcttcgg acagcctcac tcctgggggt tgctgcaact ccttccccgt gtacacgtct 12960 gcactctaac aacggagcca cagctgcact cccccctccc ccaaagcagt gtgggtattt 13020 attgatcttg ttatctgact cactgacaga ctccgggacc cacgttttag atgcattgag 13080 actcgacatt cctcggtatt tattgtctgt ccccacctac gacctccact cccgaccctt 13140 gcgaataaaa tacttctggt ctgccctaaa tcccgcgcaa tatctctgtt gtggaaagga 13200 aaccgccccg caggccaatg gagagtccaa tagagacaac caatggcttg agtgggagct 13260 agaggggagg caaagcgcac gaatcaggtt gaagggtggg gcttaggcat ccagccagta 13320 ggagagaagc aacaagccac cagagacacc accgcccccc accctccccc ccagctgtga 13380 cccagctgtg ccactcaagt ttggaaaaaa gtagggggtt gggccagcag cgggcacacc 13440 atcttcccac tgcgcctgcg caagccacgc gcatccgctt tttggaccga cactccagaa 13500 aagttgctgc aaactttcta gcgcgattcc ccgcccctcc tcccagctag atccaccgcc 13560 tacccgcggg gccgggaatt ccgaggggcg gagcacggcg cggagatggg aagggagggg 13620 gcccttcaag ggacccggga gatgggagcg gcttcgcgcc cttaaccctc cggacggccc 13680 attaccttct ccgttgctct gatagggaaa ctgaggccct gagtcagagg cacacaaggg 13740 gggaaggcca aaagcgcggc cagagacgga gggaaaacaa agaatcctga cagcccggga 13800 ggggggcgga cacacaggga caaggacaga cccgagtgca gagctgggtc tagtctttgg 13860 gagggggcca gaagactgca aggggaccgg gggggggggc ggcgaggagg actgggcgga 13920 ggagggggct ggggaagccc gcgggaggcg gcaaaggagg gaggaacttt ccaaagttgc 13980 caaacatggc tacctcgcct gcggagccga gcgcggggcc cgcggctcgg ggggaggcgg 14040 cggcggcgac cgaggagcag gaggaggaag cgcgccagct tctgcagact ctgcaggcag 14100 ccgaggggga ggcggcggcg gccggggcgg gagatgcggc ggcggcggcg gactctgggt 14160 ccccgagtgg cccggggtct ccccgggaga ccgtgaccga ggtgcccact ggccttcgct 14220 tctcgcccga acaggtggca tgcgtgtgcg aggcgctgct gcaggcgggc cacgccggcc 14280 gcttgagccg cttcctgggc gcgctgcccc cggccgagcg cctacgtggc agcgatccgg 14340 tgctgcgcgc gcgggcccta gtggccttcc agcggggtga atacgccgag ctctaccaac 14400 ttctcgagag ccgccctttc cccgccgccc accacgcctt cctgcaggac ctctacctgc 14460 gcgcgcgcta ccacgaggcc gagcgggccc gtggccgtgc gctgggcgct gtggacaaat 14520 accggctgcg caagaagttc cctctgccca agaccatctg ggatggcgag gagaccgtct 14580 attgcttcaa ggagcgctcg cgagcggcgc tcaaggcctg ctaccgcggc aaccgctatc 14640 ccacgcctga cgagaagcgc cgcctggcca cgctcaccgg cctctcgctt acacaggtca 14700 gcaactggtt caagaaccgg cgacagcgcg accgcactgg gaccggcggt ggagcgcctt 14760 gcaaaaggtg aggggggaac cgaccctcct tcctcggtgg ccgctggagt ctgcgcaagt 14820 gacccttcac atccctcttc ggtggcgtcg gcgagtgcat aggctgagcg tggagagacc 14880 aggcacaccc tgggttctct gggcatcact gcctcagggg cagaggttgt tccagctact 14940 tctaagctgg gaacgcagtg ccaggaatgg gggggggggc gggggcggga cgggcagtga 15000 <210> SEQ ID NO 4 <211> LENGTH: 1896 <212> TYPE: DNA <213> ORGANISM: mus musculus <400> SEQUENCE: 4 atgtcagccg aagtgcggct gaggcagctc cagcagctgg tgctggaccc aggcttcctg 60 ggactggagc ccctgctcga ccttctcctg ggcgtccacc aggagctggg tgcctctcac 120 ctagcccagg acaagtatgt ggccgacttc ttgcagtggg tggagcccat tgcagcaagg 180 cttaaggagg tccgactgca gagggatgat tttgagattt tgaaggtgat cgggcgtggg 240 gcgttcagcg aggtagcggt ggtgaagatg aaacagacgg gccaagtgta tgccatgaag 300 attatgaata agtgggacat gctgaagaga ggcgaggtgt cgtgcttccg ggaagaaagg 360 gatgtattag tgaaagggga ccggcgctgg atcacacagc tgcactttgc cttccaggat 420 gagaactacc tgtacctggt catggaatac tacgtgggcg gggacctgct aacgctgctg 480 agcaagtttg gggagcggat ccccgccgag atggctcgct tctacctggc cgagattgtc 540 atggccatag actccgtgca ccggctgggc tacgtgcaca gggacatcaa accagataac 600 attctgctgg accgatgtgg gcacattcgc ctggcagact tcggctcctg cctcaaactg 660 cagcctgatg gaatggtgag gtcgctggtg gctgtgggca ccccggacta cctgtctcct 720 gagattctgc aggccgttgg tggagggcct ggggcaggca gctacgggcc agagtgtgac 780 tggtgggcac tgggcgtgtt cgcctatgag atgttctatg ggcagacccc cttctacgcg 840 gactccacag ccgagacata tgccaagatt gtgcactaca gggaacactt gtcgctgccg 900 ctggcagaca cagttgtccc cgaggaagct caggacctca ttcgtgggct gctgtgtcct 960 gctgagataa ggctaggtcg aggtggggca ggtgatttcc agaaacatcc tttcttcttt 1020 ggccttgatt gggagggtct ccgagacagt gtacccccct ttacaccaga cttcgagggt 1080 gccacggaca catgcaattt cgatgtggtg gaggaccggc tcactgccat ggtgagcggg 1140 ggcggggaga cgctgtcaga catgcaggaa gacatgcccc ttggggtgcg cctgcccttc 1200 gtgggctact cctactgctg catggccttc agagacaatc aggtcccgga ccccacccct 1260 atggaactag aggccctgca gttgcctgtg tcagacttgc aagggcttga cttgcagccc 1320 ccagtgtccc caccggatca agtggctgaa gaggccgacc tagtggctgt ccctgcccct 1380 gtggctgagg cagagaccac ggtaacgctg cagcagctcc aggaagccct ggaagaagag 1440 gttctcaccc ggcagagcct gagccgcgag ctggaggcca tccggaccgc caaccagaac 1500 ttctccagcc aactacagga ggccgaggtc cgaaaccgag acctggaggc gcatgttcgg 1560 cagctacagg aacggatgga gatgctgcag gccccaggag ccgcagccat cacgggggtc 1620 cccagtcccc gggccacgga tccaccttcc catctagatg gccccccggc cgtggctgtg 1680 ggccagtgcc cgctggtggg gccaggcccc atgcaccgcc gtcacctgct gctccctgcc 1740 aggatcccta ggcctggcct atccgaggcg cgttgcctgc tcctgttcgc cgctgctctg 1800 gctgctgccg ccacactggg ctgcactggg ttggtggcct ataccggcgg tctcacccca 1860 gtctggtgtt tcccgggagc caccttcgcc ccctga 1896 <210> SEQ ID NO 5 <211> LENGTH: 771 <212> TYPE: DNA <213> ORGANISM: mus musculus <220> FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION: (89)..(89) <223> OTHER INFORMATION: n is a, c, g, or t <220> FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION: (238)..(238) <223> OTHER INFORMATION: n is a, c, g, or t <220> FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION: (506)..(506) <223> OTHER INFORMATION: n is a, c, g, or t <400> SEQUENCE: 5 cctgcccctg tggctgaggc agagaccacg gtaacgctgc agcagctcca ggaagccctg 60 gaagaagagg ttctcacccg gcagagctng agccgcgagc tggaggccat ccggaccgcc 120 aaccagaact tctccagcca actacaggag gccgaggtcc gaaaccgaga cctggaggcg 180 catgttcggc agctacagga acggatggag atgctgcagg ccccaggagc cgccggantc 240 cctcacctgc ttccagccaa gggggcactg ggtggagatg gggggcatgt tgggtgtgtg 300 aaccctcggg gcaggggagg agtccaggct ggggcaccgc gccgcgccac tgcctttctc 360 ctccatcctc cacactccat acacctctct cttctccttc cagccatcac gggggtccca 420 gtccccgggc cacggatcca ccttcccatc tagatggccc cccggcggtg gctgtgggcc 480 agtgcccgct ggtggggcca ggacantgtc accgccgtca cctgctgctc cctgccagga 540 ttcctaggcc tggctatccg aggcgcgttg ctgctcctgt tcgccgctgc tctggctgct 600 gcgccacact gggctgcact gggttggttg gctataccgg cggtcttcac ccagtctggt 660 gtttcccgtg agcacccttc gcccctgaaa cctaagactt caagccatct ttcatttagg 720 ccttctagga aggtcgagcg acaggggagc gacccaaagc gtctctgtgc c 771 <210> SEQ ID NO 6 <211> LENGTH: 434 <212> TYPE: DNA <213> ORGANISM: mus musculus <400> SEQUENCE: 6 gagagaccca aggggtagtc agggacgggc agacatgcag ctagggttct ggggcctgga 60 caggggcagc caggccctgt gacgggaaga ccccgagctc cggcccgggg aggggccatg 120 gtgttgcctg cccaacatgt cagccgaagt gcggctgagg cagctccagc agctggtgct 180 ggacccaggc ttcctgggac tggagcccct gctcgacctt ctcctgggcg tccaccagga 240 gctgggtgcc tctcacctag cccaggacaa gtatgtggcc gacttcttgc agtgggtgga 300 gcccattgca gcaaggctta aggaggtccg actgcagagg gatgattttg agattttgaa 360 ggtgatcggg cgtggggcgt tcagcgaggt agcggtggtg aagatgaaac acacggagtc 420 tttggcttcg gaca 434 <210> SEQ ID NO 7 <211> LENGTH: 2688 <212> TYPE: DNA <213> ORGANISM: mus musculus <400> SEQUENCE: 7 ccacgcgtcc gcccacgcgt ccggggcaga catgcagcta gggttctggg gcctggacag 60 gggcagccag gccctgtgac gggaagaccc cgagctccgg cccggggagg ggccatggtg 120 ttgcctgccc aacatgtcag ccgaagtgcg gctgaggcag ctccagcagc tggtgctgga 180 cccaggcttc ctgggactgg agcccctgct cgaccttctc ctgggcgtcc accaggagct 240 gggtgcctct cacctagccc aggacaagta tgtggccgac ttcttgcagt gggtggagcc 300 cattgcagca aggcttaagg aggtccgact gcagagggat gattttgaga ttttgaaggt 360 gatcgggcgt ggggcgttca gcgaggtagc ggtggtgaag atgaaacaga cgggccaagt 420 gtatgccatg aagattatga ataagtggga catgctgaag agaggcgagg tgtcgtgctt 480 ccgggaagaa agggatgtat tagtgaaagg ggaccggcgc tggatcacac agctgcactt 540 tgccttccag gatgagaact acctgtacct ggtcatggaa tactacgtgg gcggggacct 600 gctaacgctg ctgagcaagt ttggggagcg gatccccgcc gagatggctc gcttctacct 660 ggccgagatt gtcatggcca tagactccgt gcaccggctg ggctacgtgc acagggacat 720 caaaccagat aacattctgc tggaccgatg tgggcacatt cgcctggcag acttcggctc 780 ctgcctcaaa ctgcagcctg atggaatggt gaggtcgctg gtggctgtgg gcaccccgga 840 ctacctgtct cctgagattc tgcaggccgt tggtggaggg cctggggcag gcagctacgg 900 gccagagtgt gactggtggg cactgggcgt gttcacctat gagatgttct atgggcagac 960 ccccttctac gcggactcca cagccgagac atatgccaag attgtgcact acagggaaca 1020 cttgtcgctg ccgctggcag acacagttgt ccccgaggaa gctcaggacc tcattcgtgg 1080 gctgctgtgt cctgctgaga taaggctagg tcgaggtggg gcaggtgatt tccagaaaca 1140 tcctttcttc tttggccttg attgggaggg tctccgagac agtgtacccc cctttacacc 1200 agacttcgag ggtgccacgg acacatgcaa tttcgatgtg gtggaggacc ggctcactgc 1260 catggtgagc gggggcgggg agacgctgtc agacatgcag gaagacatgc cccttggggt 1320 gcgcctgccc ttcgtgggct actcctactg ctgcatggcc ttcagagaca atcaggtccc 1380 ggaccccacc cctatggaac tagaggccct gcagttgcct gtgtcagact tgcaagggct 1440 tgacttgcag cccccagtgt ccccaccgga tcaagtggct gaagaggctg acctagtggc 1500 tgtccctgcc cctgtggctg aggcagagac cacggtaacg ctgcagcagc tccaggaagc 1560 cctggaagaa gaggttctca cccggcagag cctgagccgc gagctggagg ccatccggac 1620 cgccaaccag aacttctcca gccaactaca ggaggccgag gtccgaaacc gagacctgga 1680 ggcgcatgtt cggcagctac aggaacggat ggagatgctg caggccccag gagccgcaga 1740 tccctaggcc tggcctatcc gaggcgcgtt gcctgctcct gttcgccgct gctctggctg 1800 ctgccgccac actgggctgc actgggttgg tggcctatac cggcggtctc accccagtct 1860 ggtgtttccc gggagccacc ttcgccccct gaaccctaag actccaagcc atctttcatt 1920 taggcctcct aggaaggtcg agcgaccagg gagcgaccca aagcgtctct gtgcccatcg 1980 cccccccccc cccccccacc gctccgctcc acacttctgt gagcctgggt ccccacccag 2040 ctccgctcct gtgatccagg cctgccacct ggcggccggg gagggaggaa cagggctcgt 2100 gcccagcacc cctggttcct gcagagctgg tagccaccgc tgctgcagca gctgggcatt 2160 cgccgacctt gctttactca gccccgacgt ggatgggcaa actgctcagc tcatccgatt 2220 tcactttttc actctcccag ccatcagtta caagccataa gcatgagccc cctatttcca 2280 gggacatccc attcccatag tgatggatca gcaagacctc tgccagcaca cacggagtct 2340 ttggcttcgg acagcctcac tcctgggggt tgctgcaact ccttccccgt gtacacgtct 2400 gcactctaac aacggagcca cagctgcact cccccctccc ccaaagcagt gtgggtattt 2460 attgatcttg ttatctgact cactgacaga ctccgggacc cacgttttag atgcattgag 2520 actcgacatt cctcggtatt tattgtctgt ccccacctac gacctccact cccgaccctt 2580 gcgaataaaa tacttctggt ctgccctaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa 2640 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaa 2688 <210> SEQ ID NO 8 <211> LENGTH: 2862 <212> TYPE: DNA <213> ORGANISM: mus musculus <400> SEQUENCE: 8 gggatagtca gggacgggca gacatgcagc tagggttctg gggcctggac aggggcagcc 60 aggccctgtg acgggaagac cccgagctcc ggcccgggga ggggccatgg tgttgcctgc 120 ccaacatgtc agccgaagtg cggctgaggc agctccagca gctggtgctg gacccaggct 180 tcctgggact ggagcccctg ctcgaccttc tcctgggcgt ccaccaggag ctgggtgcct 240 ctcacctagc ccaggacaag tatgtggccg acttcttgca gtgggtggag cccattgcag 300 caaggcttaa ggaggtccga ctgcagaggg atgattttga gattttgaag gtgatcgggc 360 gtggggcgtt cagcgaggta gcggtggtga agatgaaaca gacgggccaa gtgtatgcca 420 tgaagattac gaataagtgg gacatgctga agagaggcga ggtgtcgtgc ttccgggaag 480 aaagggatgt attagtgaaa ggggaccggc gctggatcac acagctgcac tttgccttcc 540 aggatgagaa ctacctgtac ctggtcatgg aatactacgt gggcggggac ctgctaacgc 600 tgctgagcaa gtttggggag cggatccccg ccgagatggc tcgcttctac ctggccgaga 660 ttgtcatggc catagactcc gtgcaccggc tgggctacgt gcacagggac atcaaaccag 720 ataacattct gctggaccga tgtgggcaca ttcgcctggc agacttcggc tcctgcctca 780 aactgcagcc tgatggaatg gtgaggtcgc tggtggctgt gggcaccccg gactacctgt 840 ctcctgagat tctgcaggcc gttggtggag ggcctggggc aggcagctac gggccagagt 900 gtgactggtg ggcactgggc gtgttcacct atgagatgtt ctatgggcag acccccttct 960 acgcggactc cacagccgag acatatgcca agattgtgca ctacagggaa cacttgtcgc 1020 tgccgctggc agacacagtt gtccccgagg aagctcagga cctcattcgt gggctgctgt 1080 gtcctgctga gataaggcta ggtcgaggtg gggcaggtga tttccagaaa catcctttct 1140 tctttggcct tgattgggag ggtctccgag acagtgtacc cccctttaca ccagacttcg 1200 agggtgccac ggacacatgc aatttcgatg tggtggagga ccggctcact gccatggaga 1260 cgctgtcaga catgcaggaa gacatgcccc ttggggtgcg cctgcccttc gtgggctact 1320 cctactgctg catggccttc agagacaatc aggtcccgga ccccacccct atggaactag 1380 aggccctgca gttgcctgtg tcagacttgc aagggcttga cttgcagccc ccagtgtccc 1440 caccggatca agtggctgaa gaggctgacc tagtggctgt ccctgcccct gtggctgagg 1500 cagagaccac ggtaacgctg cagcagctcc aggaagccct ggaagaagag gttctcaccc 1560 ggcagagcct gagccgcgag ctggaggcca tccggaccgc caaccagaac ttctccagcc 1620 aactacagga ggccgaggtc cgaaaccgag acctggaggc gcatgttcgg cagctacagg 1680 aacggatgga gatgctgcag gccccaggag ccgcagccat cacgggggtc cccagtcccc 1740 gggccacgga tccaccttcc catgcttctc gccaaatcct ccccaaggga actccctaga 1800 ctcccgttct ggcctcgact agattcccgc actgcctctc gccctgctgc tgggctccga 1860 tcgggtcacc tgtcccttct ctctccagct agatggcccc ccggccgtgg ctgtgggcca 1920 gtgcccgctg gtggggccag gccccatgca ccgccgtcac ctgctgctcc ctgccaggat 1980 ccctaggcct ggcctatccg aggcgcgttg cctgctcctg ttcgccgctg ctctggctgc 2040 tgccgccaca ctgggctgca ctgggttggt ggcctatacc ggcggtctca ccccagtctg 2100 gtgtttcccg ggagccacct tcgccccctg aaccctaaga ctccaagcca tctttcattt 2160 aggcctccta ggaaggtcga gcgaccaggg agcgacccaa agcgtctctg tgcccatcgc 2220 cccccccccc ccccccaccg ctccgctcca cacttctgtg agcctgggtc cccacccagc 2280 tccgctcctg tgatccaggc ctgccacctg gcggccgggg agggaggaac agggctcgtg 2340 cccagcaccc ctggttcctg cagagctggt agccaccgct gctgcagcag ctgggcattc 2400 gccgaccttg ctttactcag ccccgacgtg gatgggcaaa ctgctcagct catccgattt 2460 cactttttca ctctcccagc catcagttac aagccataag catgagcccc ctatttccag 2520 ggacatccca ttcccatagt gatggatcag caagacctct gccagcacac acggagtctt 2580 tggcttcgga cagcctcact cctgggggtt gctgcaactc cttccccgtg tacacgtctg 2640 cactctaaca acggagccac agctgcactc ccccctcccc caaagcagtg tgggtattta 2700 ttgatcttgt tatctgactc actgacagac tccgggaccc acgttttaga tgcattgaga 2760 ctcgacattc ctcggtattt attgtctgtc cccacctacg acctccactc ccgacccttg 2820 cgaataaaat acttctggtc tgccctaaaa aaaaaaaaaa aa 2862 <210> SEQ ID NO 9 <211> LENGTH: 2611 <212> TYPE: DNA <213> ORGANISM: mus musculus <400> SEQUENCE: 9 cgggaagacc ccgagctccg gcccggggag gggccatggt gttgcctgcc caacatgtca 60 gccgaagtgc ggctgaggca gctccagcag ctggtgctgg acccaggctt cctgggactg 120 gagcccctgc tcgaccttct cctgggcgtc caccaggagc tgggtgcctc tcacctagcc 180 caggacaagt atgtggccga cttcttgcag tgggtggagc ccattgcagc aaggcttaag 240 gaggtccgac tgcagaggga tgattttgag attttgaagg tgatcgggcg tggggcgttc 300 agcgaggtag cggtggtgaa gatgaaacag acgggccaag tgtatgccat gaagattatg 360 aataagtggg acatgctgaa gagaggcgag gtgtcgtgct tccgggaaga aagggatgta 420 ttagtgaaag gggaccggcg ctggatcaca cagctgcact ttgccttcca ggatgagaac 480 tacctgtacc tggtcatgga atactacgtg ggcggggacc tgctaacgct gctgagcaag 540 tttggggagc ggatccccgc cgagatggct cgcttctacc tggccgagat tgtcatggcc 600 atagactccg tgcaccggct gggctacgtg cacagggaca tcaaaccaga taacattctg 660 ctggaccgat gtgggcacat tcgcctggca gacttcggct cctgcctcaa actgcagcct 720 gatggaatgg tgaggtcgct ggtggctgtg ggcaccccgg actacctgtc tcctgagatt 780 ctgcaggccg ttggtggagg gcctggggca ggcagctacg ggccagagtg tgactggtgg 840 gcactgggcg tgttcaccta tgagatgttc tatgggcaga cccccttcta cgcggactcc 900 acagccgaga catatgccaa gattgtgcac tacagggaac acttgtcgct gccgctggca 960 gacacagttg tccccgagga agctcaggac ctcattcgtg ggctgctgtg tcctgctgag 1020 ataaggctag gtcgaggtgg ggcagacttc gagggtgcca cggacacatg caatttcgat 1080 gtggtggagg accggctcac tgccatggtg agcgggggcg gggagacgct gtcagacatg 1140 caggaagaca tgccccttgg ggtgcgcctg cccttcgtgg gctactccta ctgctgcatg 1200 gccttcagag acaatcaggt cccggacccc acccctatgg aactagaggc cctgcagttg 1260 cctgtgtcag acttgcaagg gcttgacttg cagcccccag tgtccccacc ggatcaagtg 1320 gctgaagagg ctgacctagt ggctgtccct gcccctgtgg ctgaggcaga gaccacggta 1380 acgctgcagc agctccagga agccctggaa gaagaggttc tcacccggca gagcctgagc 1440 cgcgagctgg aggccatccg gaccgccaac cagaacttct ccagccaact acaggaggcc 1500 gaggtccgaa accgagacct ggaggcgcat gttcggcagc tacaggaacg gatggagatg 1560 ctgcaggccc caggagccgc agccatcacg ggggtcccca gtccccgggc cacggatcca 1620 ccttcccatc tagatggccc cccggccgtg gctgtgggcc agtgcccgct ggtggggcca 1680 ggccccatgc accgccgtca cctgctgctc cctgccagga tccctaggcc tggcctatcc 1740 gaggcgcgtt gcctgctcct gttcgccgct gctctggctg ctgccgccac actgggctgc 1800 actgggttgg tggcctatac cggcggtctc accccagtct ggtgtttccc gggagccacc 1860 ttcgccccct gaaccctaag actccaagcc atctttcatt taggcctcct aggaaggtcg 1920 agcgaccagg gagcgaccca aagcgtctct gtgcccatcg cccccccccc cccccccacc 1980 gctccgctcc acacttctgt gagcctgggt ccccacccag ctccgctcct gtgatccagg 2040 cctgccacct ggcggccggg gagggaggaa cagggctcgt gcccagcacc cctggttcct 2100 gcagagctgg tagccaccgc tgctgcagca gctgggcatt cgccgacctt gctttactca 2160 gccccgacgt ggatgggcaa actgctcagc tcatccgatt tcactttttc actctcccag 2220 ccatcagtta caagccataa gcatgagccc cctatttcca gggacatccc attcccatag 2280 tgatggatca gcaagacctc tgccagcaca cacggagtct ttggcttcgg acagcctcac 2340 tcctgggggt tgctgcaact ccttccccgt gtacacgtct gcactctaac aacggagcca 2400 cagctgcact cccccctccc ccaaagcagt gtgggtattt attgatcttg ttatctgact 2460 cactgacaga ctccgggacc cacgttttag atgcattgag actcgacatt cctcggtatt 2520 tattgtctgt ccccacctac gacctccact cccgaccctt gcgaataaaa tacttctggt 2580 ctgccctaaa aaaaaaaaaa aaaaaaaaaa a 2611 <210> SEQ ID NO 10 <211> LENGTH: 988 <212> TYPE: DNA <213> ORGANISM: mus musculus <220> FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION: (531)..(531) <223> OTHER INFORMATION: n is a, c, g, or t <220> FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION: (942)..(942) <223> OTHER INFORMATION: n is a, c, g, or t <400> SEQUENCE: 10 gctggaccgg tccggaattc tccggatcgc cagcctttgt gggccatatt cgtcatccct 60 cctggcttct catctgcttt tgtggtccta gctcaagacc tctaattcct ctgctgactt 120 aaatgccctt ccccagaggt cttctcaggc ctagtggaca agcttggagc cttatctgct 180 cctgcccaac attgagccaa agctccagct taccccagct tccttacaat ggaccccatt 240 gcagcaaggc ttaaggaggt ccgactgcag agggatgatt ttgagatttt gaaggtgatc 300 gggcgtgggg cgttcagcga ggtagcggtg gtgaagatga aacagacggg ccaagtgtat 360 gccatgaaga ttatgaataa gtgggacatg ctgaagagag gcgaggtgtc gtgcttccgg 420 gaagaaaggg atgtattagt gaaaggggac cggcgctgga tcacacagct gcactttgcc 480 ttccaggatg agaactacct gtacctggtc atggaatact acgtgggcgg ngacctgcta 540 acgctgctga gcaagttttg gggagcggat ccccgccgag atggctcgct tctacctggc 600 cgagattgtc atggccatag actccgtgca ccggctgggc tacgtgcaca gggacatcaa 660 accagataac attctgctgg accgatgtgg gcacattcgc ctggcagact tcggctcctg 720 gcctcaactg cagcctgatg gaatggtgga gtcccctggt ggctgtgggc acccccggac 780 tacctgtctc ctgaaattct gcagggcctt ggtgggaggc cctggggaag gcaactacgg 840 gccaaaagtt ggaagggggg ggcctggggg gggttcccct atgaaaagtt ctatggggag 900 gacccccttt aagcggaatc ccaggccgaa aaatatgccc angattgggc cctaacaggg 960 aaaacttttc ccctgcccct gggacaat 988 <210> SEQ ID NO 11 <211> LENGTH: 649 <212> TYPE: DNA <213> ORGANISM: mus musculus <400> SEQUENCE: 11 ggcgtgttcg cctatgagat gttctatggg cagaccccct tctacgcgga ctccacagcc 60 gagacatatg ccaagattgt gcactacagg gaacacttgt cgctgccgct ggcagacaca 120 gttgtccccg aggaagctca ggacctcatt cgtgggctgc tgtgtcctgc tgagataagg 180 ctaggtcgag gtggggcagg tgatttccag aaacatcctt tcttctttgg ccttgattgg 240 gagggtctcc gagacagtgt accccccttt acaccagact tcgagggtgc cacggacaca 300 tgcaatttcg atgtggtgga ggaccggctc actgccatgg agacgctgtc agacatgcag 360 gaagacatgc cccttggggt gcgcctgccc ttcgtgggct actcctactg ctgcatggcc 420 ttcagagaca atcaggtccc ggaccccacc cctatggaac tagaggccct gcagttgcct 480 gtgtcagact tgcaagggct tgacttgcag cccccagtgt ccccaccgga tcaagtggtc 540 ccaactctga tccccaccga caggctgaag aggctgacct agtggctgtc cctgcccctg 600 tggctgaggc agagccacgg taacgctgca gcagctccag gaagccctg 649 <210> SEQ ID NO 12 <211> LENGTH: 527 <212> TYPE: DNA <213> ORGANISM: mus musculus <400> SEQUENCE: 12 atttcgatgt ggtggaggac cggctcactg ccatggtgag cgggggcggg gagacgctgt 60 cagacatgca ggaagacatg ccccttgggg tgcgcctgcc cttcgtgggc tactcctact 120 gctgcatggc cttcagagac aatcaggtcc cggaccccac ccctatggaa ctagaggccc 180 tgcagttgcc tgtgtcagac ttgcaagggc ttgacttgca gcccccagtg tccccaccgg 240 atcaagtggc tgaagaggct gacctagtgg ctgtccctgc ccctgtggct gaggcagaga 300 ccacggtaac gctgcagcag ctccaggaag ccctggaaga agaggttctc acccggcaga 360 gcctgagccg cgagctggag gccatccgga ccgccaacca gaacttctcc aggaggccga 420 ggtccgaaac cgagacctgg aggcgcatgt tcggcagcta caggaacgga tggagatgct 480 gcaggcccca ggaaccgcag ccatcacggg ggtccccagt cccccgg 527 <210> SEQ ID NO 13 <211> LENGTH: 567 <212> TYPE: DNA <213> ORGANISM: mus musculus <400> SEQUENCE: 13 atggtgaggt cgctggtggc tgtgggcacc ccggactacc tgtctcctga gattctgcag 60 gccgttggtg gagggcctgg ggcaggcagc tacgggccag agtgtgactg gtgggcactg 120 ggcgtgttcg cctatgagat gttctatggg cagaccccct tctacgcgga ctccacagcc 180 gagacatatg ccaagattgt gcactacagg gaacacttgt cgctgccgct ggcagacaca 240 gttgtccccg aggaagctca ggacctcatt cgtgggctgc tgtgtcctgc tgagataagg 300 ctaggtcgag gtggggcagg tgatttccag aaacatcctt tcttctttgg ccttgattgg 360 gagggtctcc gagacagtgt accccccttt acaccagact tcgagggtgc cacggacaca 420 tgcaatttcg atgtggtgga ggaccggctc actgccatgg tgagcggggg cggggtatga 480 ggacacacag gtgaccagtc cccaagacag tgagtgaggc ttcactcttg gcagtactaa 540 aattgaatgt agggggctgg gctcttg 567 <210> SEQ ID NO 14 <211> LENGTH: 2474 <212> TYPE: DNA <213> ORGANISM: mus musculus <400> SEQUENCE: 14 ccgggaagaa agggatgtat tagtgaaagg ggaccggcgc tggatcacac agctgcactt 60 tgccttccag gatgagaact acctgtacct ggtcatggaa tactacgtgg gcggggacct 120 gctaacgctg ctgagcaagt ttggggagcg gatccccgcc gagatggctc gcttctacct 180 ggccgagatt gtcatggcca tagactccgt gcaccggctg ggctacgtgc acagggacat 240 caaaccagat aacattctgc tggaccgatg tgggcacatt cgcctggcag acttcggctc 300 ctgcctcaaa ctgcagcctg atggaatggt gaggtcgctg gtggctgtgg gcaccccgga 360 ctacctgtct cctgagattc tgcaggccgt tggtggaggg cctggggcag gcagctacgg 420 gccagagtgt gactggtggg cactgggcgt gttcgcctat gagatgttct atgggcagac 480 ccccttctac gcggactcca cagccgagac atatgccaag attgtgcact acagggaaca 540 cttgtcgctg ccgctggcag acacagttgt ccccgaggaa gctcaggacc tcattcgtgg 600 gctgctgtgt cctgctgaga taaggctagg tcgagacttc gagggtgcca cggacacatg 660 caatttcgat gtggtggagg accggctcac tgccatggtg agcgggggcg gggagacgct 720 gtcagacatg caggaagaca tgccccttgg ggtgcgcctg cccttcgtgg gctactccta 780 ctgctgcatg gccttcagag acaatcaggt cccggacccc acccctatgg aactagaggc 840 cctgcagttg cctgtgtcag acttgcaagg gcttgacttg cagcccccag tgtccccacc 900 ggatcaagtg gctgaagagg ccgacctagt ggctgtccct gcccctgtgg ctgaggcaga 960 gaccacggta acgctgcagc agctccagga agccctggaa gaagaggttc tcacccggca 1020 gagcctgagc cgcgagctgg aggccatccg gaccgccaac cagaacttct ccagccaact 1080 acaggaggcc gaggtccgaa accgagacct ggaggcgcat gttcggcagc tacaggaacg 1140 gatggagatg ctgcaggccc caggagccgc aggcgagtcc ctcacctgct tccagccaag 1200 ggggcactgg gtggagatgg ggggcatgtt gggtgtgtga accctcgggg caggggagga 1260 gtccaggctg gggcaccgca gccgcgccac tgcctttctc ctccatcctc cacactccat 1320 acacctctct cttctccttc cagccatcac gggggtcccc agtccccggg ccacggatcc 1380 accttcccat gcttctcgcc aaatcctccc caagggaact ccctagactc ccgttctggc 1440 ctcgactaga ttcccgcact gcctctcgcc ctgctgctgg gctccgatcg ggtcacctgt 1500 cccttctctc tccagctaga tggccccccg gccgtggctg tgggccagtg cccgctggtg 1560 gggccaggcc ccatgcaccg ccgtcacctg ctgctccctg ccaggatccc taggcctggc 1620 ctatccgagg cgcgttgcct gctcctgttc gccgctgctc tggctgctgc cgccacactg 1680 ggctgcactg ggttggtggc ctataccggc ggtctcaccc cagtctggtg tttcccggga 1740 gccaccttcg ccccctgaac cctaagactc caagccatct ttcatttagg cctcctagga 1800 agatcgagcg accagggagc gacccaaagc gtctctgtgc ccatcgcccc cccccccccc 1860 cccaccgctc cgctccacac ttctgtgagc ctgggtcccc acccagctcc gctcctgtga 1920 tccaggcctg ccacctggcg gccggggagg gaggaacagg gctcgtgccc agcacccctg 1980 gttcctgcag agctggtagc caccgctgct gcagcagctg ggcattcgcc gaccttgctt 2040 tactcagccc tgacgtggat gggctaactg ctcagctcat ccgatttcac tttttcactc 2100 tcccagccat cagttacaag ccataagcat gagcccccta tttccaggga catcccattc 2160 ccatagtgat ggatcagcaa gacctctgcc agcacacacg gagtctttgg cttcggacag 2220 cctcactcct gggggttgct gcaactcctt ccccgtgtac acgtctgcac tctaacaacg 2280 gagccacagc tgcactcccc cctcccccaa agcagtgtgg gtatttattg atcttgttat 2340 ctgactcact gacagactcc gggacccacg ttttagatgc attgagactc gacattcctc 2400 ggtatttatt gtctgtcccc acctacgacc tccactcccg acccttgcga ataaaatact 2460 tctggtctgc ccta 2474 <210> SEQ ID NO 15 <211> LENGTH: 2135 <212> TYPE: DNA <213> ORGANISM: mus musculus <400> SEQUENCE: 15 ccgggaagaa agggatgtat tagtgaaagg ggaccggcgc tggatcacac agctgcactt 60 tgccttccag gatgagaact acctgtacct ggtcatggaa tactacgtgg gcggggacct 120 gctaacgctg ctgagcaagt ttggggagcg gatccccgcc gagatggctc gcttctacct 180 ggccgagatt gtcatggcca tagactccgt gcaccggctg ggctacgtgc acagggacat 240 caaaccagat aacattctgc tggaccgatg tgggcacatt cgcctggcag acttcggctc 300 ctgcctcaaa ctgcagcctg atggaatggt gaggtcgctg gtggctgtgg gcaccccgga 360 ctacctgtct cctgagattc tgcaggccgt tggtggaggg cctggggcag gcagctacgg 420 gccagagtgt gactggtggg cactgggcgt gttcgcctat gagatgttct atgggcagac 480 ccccttctac gcggactcca cagccgagac atatgccaag attgtgcact acagggaaca 540 cttgtcgctg ccgctggcag acacagttgt ccccgaggaa gctcaggacc tcattcgtgg 600 gctgctgtgt cctgctgaga taaggctagg tcgaggtggg gcaggtgatt tccagaaaca 660 tcctttcttc tttggccttg attgggaggg tctccgagac agtgtacccc cctttacacc 720 agacttcgag ggtgccacgg acacatgcaa tttcgatgtg gtggaggacc ggctcactgc 780 catggagacg ctgtcagaca tgcaggaaga catgcccctt ggggtgcgcc tgcccttcgt 840 gggctactcc tactgctgca tggccttcag agctgaagag gccgacctag tggctgtccc 900 tgcccctgtg gctgaggcag agaccacggt aacgctgcag cagctccagg aagccctgga 960 agaagaggtt ctcacccggc agagcctgag ccgcgagctg gaggccatcc ggaccgccaa 1020 ccagaacttc tccagccaac tacaggaggc cgaggtccga aaccgagacc tggaggcgca 1080 tgttcggcag ctacaggaac ggatggagat gctgcaggcc ccaggagccg cagccatcac 1140 gggggtcccc agtccccggg ccacggatcc accttcccat atggcccccc ggccgtggct 1200 gtgggccagt gcccgctggt ggggccaggc cccatgcacc gccgtcacct gctgctccct 1260 gccaggatcc ctaggcctgg cctatccgag gcgcgttgcc tgctcctgtt cgccgctgct 1320 ctggctgctg ccgccacact gggctgcact gggttggtgg cctataccgg cggtctcacc 1380 ccagtctggt gtttcccggg agccaccttc gccccctgaa ccctaagact ccaagccatc 1440 tttcatttag gcctcctagg aagatcgagc gaccagggag cgacccaaag cgtctctgtg 1500 cccatcgccc cccccccccc ccccaccgct ccgctccaca cttctgtgag cctgggtccc 1560 cacccagctc cgctcctgtg atccaggcct gccacctggc ggccggggag ggaggaacag 1620 ggctcgtgcc cagcacccct ggttcctgca gagctggtag ccaccgctgc tgcagcagct 1680 gggcattcgc cgaccttgct ttactcagcc ctgacgtgga tgggctaact gctcagctca 1740 tccgatttca ctttttcact ctcccagcca tcagttacaa gccataagca tgagccccct 1800 atttccaggg acatcccatt cccatagtga tggatcagca agacctctgc cagcacacac 1860 ggagtctttg gcttcggaca gcctcactcc tgggggttgc tgcaactcct tccccgtgta 1920 cacgtctgca ctctaacaac ggagccacag ctgcactccc ccctccccca aagcagtgtg 1980 ggtatttatt gatcttgtta tctgactcac tgacagactc cgggacccac gttttagatg 2040 cattgagact cgacattcct cggtatttat tgtctgtccc cacctacgac ctccactccc 2100 gacccttgcg aataaaatac ttctggtctg cccta 2135 <210> SEQ ID NO 16 <211> LENGTH: 2873 <212> TYPE: DNA <213> ORGANISM: homo sapiens <400> SEQUENCE: 16 aggggggctg gaccaagggg tggggagaag gggaggaggc ctcggccggc cgcagagaga 60 agtggccaga gaggcccagg ggacagccag ggacaggcag acatgcagcc agggctccag 120 ggcctggaca ggggctgcca ggccctgtga caggaggacc ccgagccccc ggcccgggga 180 ggggccatgg tgctgcctgt ccaacatgtc agccgaggtg cggctgaggc ggctccagca 240 gctggtgttg gacccgggct tcctggggct ggagcccctg ctcgaccttc tcctgggcgt 300 ccaccaggag ctgggcgcct ccgaactggc ccaggacaag tacgtggccg acttcttgca 360 gtgggcggag cccatcgtgg tgaggcttaa ggaggtccga ctgcagaggg acgacttcga 420 gattctgaag gtgatcggac gcggggcgtt cagcgaggta gcggtagtga agatgaagca 480 gacgggccag gtgtatgcca tgaagatcat gaacaagtgg gacatgctga agaggggcga 540 ggtgtcgtgc ttccgtgagg agagggacgt gttggtgaat ggggaccggc ggtggatcac 600 gcagctgcac ttcgccttcc aggatgagaa ctacctgtac ctggtcatgg agtattacgt 660 gggcggggac ctgctgacac tgctgagcaa gtttggggag cggattccgg ccgagatggc 720 gcgcttctac ctggcggaga ttgtcatggc catagactcg gtgcaccggc ttggctacgt 780 gcacagggac atcaaacccg acaacatcct gctggaccgc tgtggccaca tccgcctggc 840 cgacttcggc tcttgcctca agctgcgggc agatggaacg gtgcggtcgc tggtggctgt 900 gggcacccca gactacctgt cccccgagat cctgcaggct gtgggcggtg ggcctgggac 960 aggcagctac gggcccgagt gtgactggtg ggcgctgggt gtattcgcct atgaaatgtt 1020 ctatgggcag acgcccttct acgcggattc cacggcggag acctatggca agatcgtcca 1080 ctacaaggag cacctctctc tgccgctggt ggacgaaggg gtccctgagg aggctcgaga 1140 cttcattcag cggttgctgt gtcccccgga gacacggctg ggccggggtg gagcaggcga 1200 cttccggaca catcccttct tctttggcct cgactgggat ggtctccggg acagcgtgcc 1260 cccctttaca ccggatttcg aaggtgccac cgacacatgc aacttcgact tggtggagga 1320 cgggctcact gccatggaga cactgtcgga cattcgggaa ggtgcgccgc taggggtcca 1380 cctgcctttt gtgggctact cctactcctg catggccctc agggacagtg aggtcccagg 1440 ccccacaccc atggaactgg aggccgagca gctgcttgag ccacacgtgc aagcgcccag 1500 cctggagccc tcggtgtccc cacaggatga aacagctgaa gtggcagttc cagcggctgt 1560 ccctgcggca gaggctgagg ccgaggtgac gctgcgggag ctccaggaag ccctggagga 1620 ggaggtgctc acccggcaga gcctgagccg ggagatggag gccatccgca cggacaacca 1680 gaacttcgcc agtcaactac gcgaggcaga ggctcggaac cgggacctag aggcacacgt 1740 ccggcagttg caggagcgga tggagttgct gcaggcagag ggagccacag ctgtcacggg 1800 ggtccccagt ccccgggcca cggatccacc ttcccatatg gccccccggc cgtggctgtg 1860 ggccagtgcc cgctggtggg gccaggcccc atgcaccgcc gccacctgct gctccctgcc 1920 agggtcccta ggcctggcct atcggaggcg ctttccctgc tcctgttcgc cgttgttctg 1980 tctcgtgccg ccgccctggg ctgcattggg ttggtggccc acgccggcca actcaccgca 2040 gtctggcgcc gcccaggagc cgcccgcgct ccctgaaccc tagaactgtc ttcgactccg 2100 gggccccgtt ggaagactga gtgcccgggg cacggcacag aagccgcgcc caccgcctgc 2160 cagttcacaa ccgctccgag cgtgggtctc cgcccagctc cagtcctgtg atccgggccc 2220 gccccctagc ggccggggag ggaggggccg ggtccgcggc cggcgaacgg ggctcgaagg 2280 gtccttgtag ccgggaatgc tgctgctgct gctgctgctg ctgctgctgc tgctgctgct 2340 gctgctgctg ctgctgctgg ggggatcaca gaccatttct ttctttcggc caggctgagg 2400 ccctgacgtg gatgggcaaa ctgcaggcct gggaaggcag caagccgggc cgtccgtgtt 2460 ccatcctcca cgcaccccca cctatcgttg gttcgcaaag tgcaaagctt tcttgtgcat 2520 gacgccctgc tctggggagc gtctggcgcg atctctgcct gcttactcgg gaaatttgct 2580 tttgccaaac ccgctttttc ggggatcccg cgcccccctc ctcacttgcg ctgctctcgg 2640 agccccagcc ggctccgccc gcttcggcgg tttggatatt tattgacctc gtcctccgac 2700 tcgctgacag gctacaggac ccccaacaac cccaatccac gttttggatg cactgagacc 2760 ccgacattcc tcggtattta ttgtctgtcc ccacctagga cccccacccc cgaccctcgc 2820 gaataaaagg ccctccatct gcccaaaaaa aaaaaaaaaa aaaaaaaaaa aaa 2873 <210> SEQ ID NO 17 <211> LENGTH: 1509 <212> TYPE: DNA <213> ORGANISM: homo sapiens <400> SEQUENCE: 17 ccaccgcagc ggacagcgcc aagtgaagcc tcgcttcccc tccgcggcga ccagggcccg 60 agccgagagt agcagttgta gctacccgcc cagaaactag acacaatgtg cgacgaagac 120 gagaccaccg ccctcgtgtg cgacaatggc tccggcctgg tgaaagccgg cttcgccggg 180 gatgacgccc ctagggccgt gttcccgtcc atcgtgggcc gcccccgaca ccagggcgtc 240 atggtcggta tgggtcagaa agattcctac gtgggcgacg aggctcagag caagagaggt 300 atcctgaccc tgaagtaccc tatcgagcac ggcatcatca ccaactggga tgacatggag 360 aagatctggc accacacctt ctacaacgag cttcgcgtgg ctcccgagga gcaccccacc 420 ctgctcaccg aggcccccct caatcccaag gccaaccgcg agaagatgac ccagatcatg 480 tttgagacct tcaacgtgcc cgccatgtac gtggccatcc aggccgtgct gtccctctac 540 gcctccggca ggaccaccgg catcgtgctg gactccggcg acggcgtcac ccacaacgtg 600 cccatttatg agggctacgc gctgccgcac gccatcatgc gcctggacct ggcgggccgc 660 gatctcaccg actacctgat gaagatcctc actgagcgtg gctactcctt cgtgaccaca 720 gctgagcgcg agatcgtgcg cgacatcaag gagaagctgt gctacgtggc cctggacttc 780 gagaacgaga tggcgacggc cgcctcctcc tcctccctgg aaaagagcta cgagctgcca 840 gacgggcagg tcatcaccat cggcaacgag cgcttccgct gcccggagac gctcttccag 900 ccctccttca tcggtatgga gtcggcgggc attcacgaga ccacctacaa cagcatcatg 960 aagtgtgaca tcgacatcag gaaggacctg tatgccaaca acgtcatgtc ggggggcacc 1020 acgatgtacc ctgggatcgc tgaccgcatg cagaaagaga tcaccgcgct ggcacccagc 1080 accatgaaga tcaagatcat cgccccgccg gagcgcaaat actcggtgtg gatcggcggc 1140 tccatcctgg cctcgctgtc caccttccag cagatgtgga tcaccaagca ggagtacgac 1200 gaggccggcc cttccatcgt ccaccgcaaa tgcttctaga cacactccac ctccagcacg 1260 cgacttctca ggacgacgaa tcttctcaat gggggggcgg ctgagctcca gccaccccgc 1320 agtcactttc tttgtaacaa cttccgttgc tgccatcgta aactgacaca gtgtttataa 1380 cgtgtacata cattaactta ttacctcatt ttgttatttt tcgaaacaaa gccctgtgga 1440 agaaaatgga aaacttgaag aagcattaaa gtcattctgt taagctgcgt aaaaaaaaaa 1500 aaaaaaaaa 1509 <210> SEQ ID NO 18 <400> SEQUENCE: 18 000 <210> SEQ ID NO 19 <400> SEQUENCE: 19 000 <210> SEQ ID NO 20 <211> LENGTH: 18 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Primer <400> SEQUENCE: 20 agcctgagcc gggagatg 18 <210> SEQ ID NO 21 <211> LENGTH: 21 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Primer <400> SEQUENCE: 21 gcgtagttga ctggcgaagt t 21 <210> SEQ ID NO 22 <211> LENGTH: 21 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Probe <400> SEQUENCE: 22 aggccatccg cacggacaac c 21 <210> SEQ ID NO 23 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 23 acaataaata ccgagg 16 <210> SEQ ID NO 24 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 24 cggagcggtt gtgaactggc 20 <210> SEQ ID NO 25 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 25 gcgcaccttc ccgaatgtcc 20 <210> SEQ ID NO 26 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 26 cccgaatgtc cgacag 16 <210> SEQ ID NO 27 <211> LENGTH: 17 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 27 cggagcggtt gtgaact 17 <210> SEQ ID NO 28 <211> LENGTH: 17 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 28 acaataaata ccgagga 17 <210> SEQ ID NO 29 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 29 gacaatctcc gccagg 16 <210> SEQ ID NO 30 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 30 tcccgaatgt ccgaca 16 <210> SEQ ID NO 31 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 31 tgtaatgttg tccagt 16 <210> SEQ ID NO 32 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 32 gtgtaatgtt gtccag 16 <210> SEQ ID NO 33 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 33 ttgcactttg cgaaccaacg 20 <210> SEQ ID NO 34 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 34 cgacacctcg cccctcttca 20 <210> SEQ ID NO 35 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 35 acgacacctc gcccctcttc 20 <210> SEQ ID NO 36 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 36 cacgacacct cgcccctctt 20 <210> SEQ ID NO 37 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 37 gcacgacacc tcgcccctct 20 <210> SEQ ID NO 38 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 38 agcacgacac ctcgcccctc 20 <210> SEQ ID NO 39 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 39 aagcacgaca cctcgcccct 20 <210> SEQ ID NO 40 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 40 gaagcacgac acctcgcccc 20 <210> SEQ ID NO 41 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 41 ggaagcacga cacctcgccc 20 <210> SEQ ID NO 42 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 42 cggaagcacg acacctcgcc 20 <210> SEQ ID NO 43 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 43 acggaagcac gacacctcgc 20 <210> SEQ ID NO 44 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 44 cacggaagca cgacacctcg 20 <210> SEQ ID NO 45 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 45 tcacggaagc acgacacctc 20 <210> SEQ ID NO 46 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 46 ctcacggaag cacgacacct 20 <210> SEQ ID NO 47 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 47 cctcacggaa gcacgacacc 20 <210> SEQ ID NO 48 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 48 tcctcacgga agcacgacac 20 <210> SEQ ID NO 49 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 49 ctcctcacgg aagcacgaca 20 <210> SEQ ID NO 50 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 50 tctcctcacg gaagcacgac 20 <210> SEQ ID NO 51 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 51 ctctcctcac ggaagcacga 20 <210> SEQ ID NO 52 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 52 cctctcctca cggaagcacg 20 <210> SEQ ID NO 53 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 53 ccctctcctc acggaagcac 20 <210> SEQ ID NO 54 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 54 tccctctcct cacggaagca 20 <210> SEQ ID NO 55 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 55 gtccctctcc tcacggaagc 20 <210> SEQ ID NO 56 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 56 cgtccctctc ctcacggaag 20 <210> SEQ ID NO 57 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 57 ggtccccatt caccaacacg 20 <210> SEQ ID NO 58 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 58 cggtccccat tcaccaacac 20 <210> SEQ ID NO 59 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 59 ccggtcccca ttcaccaaca 20 <210> SEQ ID NO 60 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 60 gccggtcccc attcaccaac 20 <210> SEQ ID NO 61 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 61 cgccggtccc cattcaccaa 20 <210> SEQ ID NO 62 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 62 ccgccggtcc ccattcacca 20 <210> SEQ ID NO 63 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 63 accgccggtc cccattcacc 20 <210> SEQ ID NO 64 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 64 caccgccggt ccccattcac 20 <210> SEQ ID NO 65 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 65 ccaccgccgg tccccattca 20 <210> SEQ ID NO 66 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 66 tccaccgccg gtccccattc 20 <210> SEQ ID NO 67 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 67 atccaccgcc ggtccccatt 20 <210> SEQ ID NO 68 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 68 gatccaccgc cggtccccat 20 <210> SEQ ID NO 69 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 69 tgatccaccg ccggtcccca 20 <210> SEQ ID NO 70 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 70 gtgatccacc gccggtcccc 20 <210> SEQ ID NO 71 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 71 cgtgatccac cgccggtccc 20 <210> SEQ ID NO 72 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 72 ttctcatcct ggaaggcgaa 20 <210> SEQ ID NO 73 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 73 gttctcatcc tggaaggcga 20 <210> SEQ ID NO 74 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 74 agttctcatc ctggaaggcg 20 <210> SEQ ID NO 75 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 75 gtagttctca tcctggaagg 20 <210> SEQ ID NO 76 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 76 ggtagttctc atcctggaag 20 <210> SEQ ID NO 77 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 77 aggtagttct catcctggaa 20 <210> SEQ ID NO 78 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 78 caggtagttc tcatcctgga 20 <210> SEQ ID NO 79 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 79 tacaggtagt tctcatcctg 20 <210> SEQ ID NO 80 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 80 gtacaggtag ttctcatcct 20 <210> SEQ ID NO 81 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 81 ggtacaggta gttctcatcc 20 <210> SEQ ID NO 82 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 82 aggtacaggt agttctcatc 20 <210> SEQ ID NO 83 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 83 ccaggtacag gtagttctca 20 <210> SEQ ID NO 84 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 84 accaggtaca ggtagttctc 20 <210> SEQ ID NO 85 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 85 gaccaggtac aggtagttct 20 <210> SEQ ID NO 86 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 86 tgaccaggta caggtagttc 20 <210> SEQ ID NO 87 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 87 catgaccagg tacaggtagt 20 <210> SEQ ID NO 88 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 88 ccatgaccag gtacaggtag 20 <210> SEQ ID NO 89 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 89 tccatgacca ggtacaggta 20 <210> SEQ ID NO 90 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 90 ctccatgacc aggtacaggt 20 <210> SEQ ID NO 91 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 91 actccatgac caggtacagg 20 <210> SEQ ID NO 92 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 92 tactccatga ccaggtacag 20 <210> SEQ ID NO 93 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 93 atactccatg accaggtaca 20 <210> SEQ ID NO 94 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 94 aatactccat gaccaggtac 20 <210> SEQ ID NO 95 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 95 taatactcca tgaccaggta 20 <210> SEQ ID NO 96 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 96 cgtaatactc catgaccagg 20 <210> SEQ ID NO 97 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 97 agcagtgtca gcaggtcccc 20 <210> SEQ ID NO 98 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 98 cagcagtgtc agcaggtccc 20 <210> SEQ ID NO 99 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 99 tcagcagtgt cagcaggtcc 20 <210> SEQ ID NO 100 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 100 ctcagcagtg tcagcaggtc 20 <210> SEQ ID NO 101 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 101 gctcagcagt gtcagcaggt 20 <210> SEQ ID NO 102 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 102 tgctcagcag tgtcagcagg 20 <210> SEQ ID NO 103 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 103 ttgctcagca gtgtcagcag 20 <210> SEQ ID NO 104 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 104 cttgctcagc agtgtcagca 20 <210> SEQ ID NO 105 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 105 acttgctcag cagtgtcagc 20 <210> SEQ ID NO 106 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 106 aacttgctca gcagtgtcag 20 <210> SEQ ID NO 107 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 107 aaacttgctc agcagtgtca 20 <210> SEQ ID NO 108 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 108 caaacttgct cagcagtgtc 20 <210> SEQ ID NO 109 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 109 ccaaacttgc tcagcagtgt 20 <210> SEQ ID NO 110 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 110 cccaaacttg ctcagcagtg 20 <210> SEQ ID NO 111 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 111 gtgagcccgt cctccaccaa 20 <210> SEQ ID NO 112 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 112 agtgagcccg tcctccacca 20 <210> SEQ ID NO 113 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 113 cagtgagccc gtcctccacc 20 <210> SEQ ID NO 114 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 114 gcagtgagcc cgtcctccac 20 <210> SEQ ID NO 115 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 115 ggcagtgagc ccgtcctcca 20 <210> SEQ ID NO 116 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 116 tggcagtgag cccgtcctcc 20 <210> SEQ ID NO 117 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 117 catggcagtg agcccgtcct 20 <210> SEQ ID NO 118 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 118 ccatggcagt gagcccgtcc 20 <210> SEQ ID NO 119 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 119 tccatggcag tgagcccgtc 20 <210> SEQ ID NO 120 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 120 ctccatggca gtgagcccgt 20 <210> SEQ ID NO 121 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 121 tctccatggc agtgagcccg 20 <210> SEQ ID NO 122 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 122 gtctccatgg cagtgagccc 20 <210> SEQ ID NO 123 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 123 ccttcccgaa tgtccgacag 20 <210> SEQ ID NO 124 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 124 accttcccga atgtccgaca 20 <210> SEQ ID NO 125 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 125 caccttcccg aatgtccgac 20 <210> SEQ ID NO 126 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 126 gcaccttccc gaatgtccga 20 <210> SEQ ID NO 127 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 127 cgcaccttcc cgaatgtccg 20 <210> SEQ ID NO 128 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 128 ggcgcacctt cccgaatgtc 20 <210> SEQ ID NO 129 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 129 acaaaaggca ggtggacccc 20 <210> SEQ ID NO 130 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 130 cacaaaaggc aggtggaccc 20 <210> SEQ ID NO 131 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 131 ccacaaaagg caggtggacc 20 <210> SEQ ID NO 132 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 132 cccacaaaag gcaggtggac 20 <210> SEQ ID NO 133 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 133 gcccacaaaa ggcaggtgga 20 <210> SEQ ID NO 134 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 134 agcccacaaa aggcaggtgg 20 <210> SEQ ID NO 135 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 135 tagcccacaa aaggcaggtg 20 <210> SEQ ID NO 136 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 136 gtagcccaca aaaggcaggt 20 <210> SEQ ID NO 137 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 137 agtagcccac aaaaggcagg 20 <210> SEQ ID NO 138 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 138 gagtagccca caaaaggcag 20 <210> SEQ ID NO 139 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 139 ggagtagccc acaaaaggca 20 <210> SEQ ID NO 140 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 140 aggagtagcc cacaaaaggc 20 <210> SEQ ID NO 141 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 141 taggagtagc ccacaaaagg 20 <210> SEQ ID NO 142 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 142 gtaggagtag cccacaaaag 20 <210> SEQ ID NO 143 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 143 agtaggagta gcccacaaaa 20 <210> SEQ ID NO 144 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 144 gagtaggagt agcccacaaa 20 <210> SEQ ID NO 145 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 145 ggagtaggag tagcccacaa 20 <210> SEQ ID NO 146 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 146 aggagtagga gtagcccaca 20 <210> SEQ ID NO 147 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 147 caggagtagg agtagcccac 20 <210> SEQ ID NO 148 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 148 gcaggagtag gagtagccca 20 <210> SEQ ID NO 149 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 149 tgcaggagta ggagtagccc 20 <210> SEQ ID NO 150 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 150 atgcaggagt aggagtagcc 20 <210> SEQ ID NO 151 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 151 catgcaggag taggagtagc 20 <210> SEQ ID NO 152 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 152 ccatgcagga gtaggagtag 20 <210> SEQ ID NO 153 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 153 gccatgcagg agtaggagta 20 <210> SEQ ID NO 154 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 154 ggccatgcag gagtaggagt 20 <210> SEQ ID NO 155 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 155 gggccatgca ggagtaggag 20 <210> SEQ ID NO 156 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 156 agggccatgc aggagtagga 20 <210> SEQ ID NO 157 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 157 gagggccatg caggagtagg 20 <210> SEQ ID NO 158 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 158 ctgagggcca tgcaggagta 20 <210> SEQ ID NO 159 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 159 cctgagggcc atgcaggagt 20 <210> SEQ ID NO 160 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 160 ccctgagggc catgcaggag 20 <210> SEQ ID NO 161 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 161 tccctgaggg ccatgcagga 20 <210> SEQ ID NO 162 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 162 gtccctgagg gccatgcagg 20 <210> SEQ ID NO 163 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 163 tgtccctgag ggccatgcag 20 <210> SEQ ID NO 164 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 164 ctgtccctga gggccatgca 20 <210> SEQ ID NO 165 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 165 actgtccctg agggccatgc 20 <210> SEQ ID NO 166 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 166 cactgtccct gagggccatg 20 <210> SEQ ID NO 167 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 167 tcactgtccc tgagggccat 20 <210> SEQ ID NO 168 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 168 ctcactgtcc ctgagggcca 20 <210> SEQ ID NO 169 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 169 cctcactgtc cctgagggcc 20 <210> SEQ ID NO 170 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 170 acctcactgt ccctgagggc 20 <210> SEQ ID NO 171 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 171 gacctcactg tccctgaggg 20 <210> SEQ ID NO 172 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 172 ggacctcact gtccctgagg 20 <210> SEQ ID NO 173 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 173 gggacctcac tgtccctgag 20 <210> SEQ ID NO 174 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 174 cctccagttc catgggtgtg 20 <210> SEQ ID NO 175 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 175 gcctccagtt ccatgggtgt 20 <210> SEQ ID NO 176 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 176 ggcctccagt tccatgggtg 20 <210> SEQ ID NO 177 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 177 cggcctccag ttccatgggt 20 <210> SEQ ID NO 178 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 178 tcggcctcca gttccatggg 20 <210> SEQ ID NO 179 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 179 ctcggcctcc agttccatgg 20 <210> SEQ ID NO 180 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 180 gctcggcctc cagttccatg 20 <210> SEQ ID NO 181 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 181 tgctcggcct ccagttccat 20 <210> SEQ ID NO 182 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 182 ctgctcggcc tccagttcca 20 <210> SEQ ID NO 183 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 183 gctgctcggc ctccagttcc 20 <210> SEQ ID NO 184 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 184 agctgctcgg cctccagttc 20 <210> SEQ ID NO 185 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 185 cagctgctcg gcctccagtt 20 <210> SEQ ID NO 186 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 186 gcagctgctc ggcctccagt 20 <210> SEQ ID NO 187 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 187 agcagctgct cggcctccag 20 <210> SEQ ID NO 188 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 188 aagcagctgc tcggcctcca 20 <210> SEQ ID NO 189 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 189 caagcagctg ctcggcctcc 20 <210> SEQ ID NO 190 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 190 tcaagcagct gctcggcctc 20 <210> SEQ ID NO 191 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 191 ctcaagcagc tgctcggcct 20 <210> SEQ ID NO 192 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 192 gctcaagcag ctgctcggcc 20 <210> SEQ ID NO 193 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 193 ggctcaagca gctgctcggc 20 <210> SEQ ID NO 194 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 194 tggctcaagc agctgctcgg 20 <210> SEQ ID NO 195 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 195 gtggctcaag cagctgctcg 20 <210> SEQ ID NO 196 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 196 tgtggctcaa gcagctgctc 20 <210> SEQ ID NO 197 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 197 gtgtggctca agcagctgct 20 <210> SEQ ID NO 198 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 198 ccacttcagc tgtttcatcc 20 <210> SEQ ID NO 199 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 199 tgccacttca gctgtttcat 20 <210> SEQ ID NO 200 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 200 ctgccacttc agctgtttca 20 <210> SEQ ID NO 201 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 201 actgccactt cagctgtttc 20 <210> SEQ ID NO 202 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 202 aactgccact tcagctgttt 20 <210> SEQ ID NO 203 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 203 gaactgccac ttcagctgtt 20 <210> SEQ ID NO 204 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 204 ggaactgcca cttcagctgt 20 <210> SEQ ID NO 205 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 205 tggaactgcc acttcagctg 20 <210> SEQ ID NO 206 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 206 ctggaactgc cacttcagct 20 <210> SEQ ID NO 207 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 207 gctggaactg ccacttcagc 20 <210> SEQ ID NO 208 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 208 cgctggaact gccacttcag 20 <210> SEQ ID NO 209 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 209 ccgctggaac tgccacttca 20 <210> SEQ ID NO 210 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 210 gccgctggaa ctgccacttc 20 <210> SEQ ID NO 211 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 211 agccgctgga actgccactt 20 <210> SEQ ID NO 212 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 212 ctcagcctct gccgcaggga 20 <210> SEQ ID NO 213 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 213 cctcagcctc tgccgcaggg 20 <210> SEQ ID NO 214 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 214 ggcctcagcc tctgccgcag 20 <210> SEQ ID NO 215 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 215 cggcctcagc ctctgccgca 20 <210> SEQ ID NO 216 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 216 tcggcctcag cctctgccgc 20 <210> SEQ ID NO 217 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 217 ctcggcctca gcctctgccg 20 <210> SEQ ID NO 218 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 218 cctcggcctc agcctctgcc 20 <210> SEQ ID NO 219 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 219 acctcggcct cagcctctgc 20 <210> SEQ ID NO 220 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 220 cacctcggcc tcagcctctg 20 <210> SEQ ID NO 221 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 221 tcacctcggc ctcagcctct 20 <210> SEQ ID NO 222 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 222 gtcacctcgg cctcagcctc 20 <210> SEQ ID NO 223 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 223 cgtcacctcg gcctcagcct 20 <210> SEQ ID NO 224 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 224 agcacctcct cctccagggc 20 <210> SEQ ID NO 225 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 225 gagcacctcc tcctccaggg 20 <210> SEQ ID NO 226 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 226 tgagcacctc ctcctccagg 20 <210> SEQ ID NO 227 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 227 gtgagcacct cctcctccag 20 <210> SEQ ID NO 228 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 228 ggtgagcacc tcctcctcca 20 <210> SEQ ID NO 229 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 229 gggtgagcac ctcctcctcc 20 <210> SEQ ID NO 230 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 230 cgggtgagca cctcctcctc 20 <210> SEQ ID NO 231 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 231 ccgggtgagc acctcctcct 20 <210> SEQ ID NO 232 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 232 gccgggtgag cacctcctcc 20 <210> SEQ ID NO 233 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 233 tgccgggtga gcacctcctc 20 <210> SEQ ID NO 234 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 234 ctgccgggtg agcacctcct 20 <210> SEQ ID NO 235 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 235 tctgccgggt gagcacctcc 20 <210> SEQ ID NO 236 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 236 gctctgccgg gtgagcacct 20 <210> SEQ ID NO 237 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 237 ggctctgccg ggtgagcacc 20 <210> SEQ ID NO 238 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 238 aggctctgcc gggtgagcac 20 <210> SEQ ID NO 239 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 239 caggctctgc cgggtgagca 20 <210> SEQ ID NO 240 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 240 tcaggctctg ccgggtgagc 20 <210> SEQ ID NO 241 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 241 gctcaggctc tgccgggtga 20 <210> SEQ ID NO 242 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 242 cggctcaggc tctgccgggt 20 <210> SEQ ID NO 243 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 243 ccggctcagg ctctgccggg 20 <210> SEQ ID NO 244 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 244 cccggctcag gctctgccgg 20 <210> SEQ ID NO 245 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 245 tcccggctca ggctctgccg 20 <210> SEQ ID NO 246 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 246 ctcccggctc aggctctgcc 20 <210> SEQ ID NO 247 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 247 tctcccggct caggctctgc 20 <210> SEQ ID NO 248 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 248 atctcccggc tcaggctctg 20 <210> SEQ ID NO 249 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 249 catctcccgg ctcaggctct 20 <210> SEQ ID NO 250 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 250 ccatctcccg gctcaggctc 20 <210> SEQ ID NO 251 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 251 tccatctccc ggctcaggct 20 <210> SEQ ID NO 252 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 252 ctccatctcc cggctcaggc 20 <210> SEQ ID NO 253 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 253 cctccatctc ccggctcagg 20 <210> SEQ ID NO 254 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 254 gcctccatct cccggctcag 20 <210> SEQ ID NO 255 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 255 ggcctccatc tcccggctca 20 <210> SEQ ID NO 256 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 256 tggcctccat ctcccggctc 20 <210> SEQ ID NO 257 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 257 atggcctcca tctcccggct 20 <210> SEQ ID NO 258 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 258 gatggcctcc atctcccggc 20 <210> SEQ ID NO 259 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 259 ggatggcctc catctcccgg 20 <210> SEQ ID NO 260 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 260 cggatggcct ccatctcccg 20 <210> SEQ ID NO 261 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 261 gcggatggcc tccatctccc 20 <210> SEQ ID NO 262 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 262 tgcggatggc ctccatctcc 20 <210> SEQ ID NO 263 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 263 gttccgagcc tctgcctcgc 20 <210> SEQ ID NO 264 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 264 gtcggaggac gaggtcaata 20 <210> SEQ ID NO 265 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 265 agggcctcag cctggccgaa 20 <210> SEQ ID NO 266 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 266 cagggcctca gcctggccga 20 <210> SEQ ID NO 267 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 267 gtcagggcct cagcctggcc 20 <210> SEQ ID NO 268 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 268 cgtcagggcc tcagcctggc 20 <210> SEQ ID NO 269 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 269 agcaaatttc ccgagtaagc 20 <210> SEQ ID NO 270 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 270 aagcaaattt cccgagtaag 20 <210> SEQ ID NO 271 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 271 aaaagcaaat ttcccgagta 20 <210> SEQ ID NO 272 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 272 caaaagcaaa tttcccgagt 20 <210> SEQ ID NO 273 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 273 gcaaaagcaa atttcccgag 20 <210> SEQ ID NO 274 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 274 ggcaaaagca aatttcccga 20 <210> SEQ ID NO 275 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 275 tggcaaaagc aaatttcccg 20 <210> SEQ ID NO 276 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 276 tttggcaaaa gcaaatttcc 20 <210> SEQ ID NO 277 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 277 gtttggcaaa agcaaatttc 20 <210> SEQ ID NO 278 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 278 gggtttggca aaagcaaatt 20 <210> SEQ ID NO 279 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 279 cgggtttggc aaaagcaaat 20 <210> SEQ ID NO 280 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 280 aagcgggttt ggcaaaagca 20 <210> SEQ ID NO 281 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 281 aatatccaaa ccgccgaagc 20 <210> SEQ ID NO 282 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 282 aaatatccaa accgccgaag 20 <210> SEQ ID NO 283 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 283 ataaatatcc aaaccgccga 20 <210> SEQ ID NO 284 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 284 aataaatatc caaaccgccg 20 <210> SEQ ID NO 285 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 285 tcaataaata tccaaaccgc 20 <210> SEQ ID NO 286 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 286 gtcaataaat atccaaaccg 20 <210> SEQ ID NO 287 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 287 ggtcaataaa tatccaaacc 20 <210> SEQ ID NO 288 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 288 aggtcaataa atatccaaac 20 <210> SEQ ID NO 289 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 289 gaggtcaata aatatccaaa 20 <210> SEQ ID NO 290 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 290 acgaggtcaa taaatatcca 20 <210> SEQ ID NO 291 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 291 gacgaggtca ataaatatcc 20 <210> SEQ ID NO 292 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 292 aggacgaggt caataaatat 20 <210> SEQ ID NO 293 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 293 gaggacgagg tcaataaata 20 <210> SEQ ID NO 294 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 294 cggaggacga ggtcaataaa 20 <210> SEQ ID NO 295 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 295 tcggaggacg aggtcaataa 20 <210> SEQ ID NO 296 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 296 agtcggagga cgaggtcaat 20 <210> SEQ ID NO 297 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 297 gagtcggagg acgaggtcaa 20 <210> SEQ ID NO 298 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 298 gcgagtcgga ggacgaggtc 20 <210> SEQ ID NO 299 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 299 agcgagtcgg aggacgaggt 20 <210> SEQ ID NO 300 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 300 cagcgagtcg gaggacgagg 20 <210> SEQ ID NO 301 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 301 tcagcgagtc ggaggacgag 20 <210> SEQ ID NO 302 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 302 gtcagcgagt cggaggacga 20 <210> SEQ ID NO 303 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 303 ctgtcagcga gtcggaggac 20 <210> SEQ ID NO 304 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 304 cctgtcagcg agtcggagga 20 <210> SEQ ID NO 305 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 305 agcctgtcag cgagtcggag 20 <210> SEQ ID NO 306 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 306 gtctcagtgc atccaaaacg 20 <210> SEQ ID NO 307 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 307 ggtctcagtg catccaaaac 20 <210> SEQ ID NO 308 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 308 gggtctcagt gcatccaaaa 20 <210> SEQ ID NO 309 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 309 ggagggcctt ttattcgcga 20 <210> SEQ ID NO 310 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 310 tggagggcct tttattcgcg 20 <210> SEQ ID NO 311 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 311 atggagggcc ttttattcgc 20 <210> SEQ ID NO 312 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 312 gatggagggc cttttattcg 20 <210> SEQ ID NO 313 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 313 agatggaggg ccttttattc 20 <210> SEQ ID NO 314 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 314 cagatggagg gccttttatt 20 <210> SEQ ID NO 315 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 315 gcagatggag ggccttttat 20 <210> SEQ ID NO 316 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 316 ccctcaggct ctctgcttta 20 <210> SEQ ID NO 317 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 317 gccctcaggc tctctgcttt 20 <210> SEQ ID NO 318 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 318 agccctcagg ctctctgctt 20 <210> SEQ ID NO 319 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 319 tagccctcag gctctctgct 20 <210> SEQ ID NO 320 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 320 ttagccctca ggctctctgc 20 <210> SEQ ID NO 321 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 321 tttagccctc aggctctctg 20 <210> SEQ ID NO 322 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 322 atttagccct caggctctct 20 <210> SEQ ID NO 323 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 323 aatttagccc tcaggctctc 20 <210> SEQ ID NO 324 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 324 aaatttagcc ctcaggctct 20 <210> SEQ ID NO 325 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 325 taaatttagc cctcaggctc 20 <210> SEQ ID NO 326 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 326 ttaaatttag ccctcaggct 20 <210> SEQ ID NO 327 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 327 gttaaattta gccctcaggc 20 <210> SEQ ID NO 328 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 328 agttaaattt agccctcagg 20 <210> SEQ ID NO 329 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 329 cagttaaatt tagccctcag 20 <210> SEQ ID NO 330 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 330 acagttaaat ttagccctca 20 <210> SEQ ID NO 331 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 331 gacagttaaa tttagccctc 20 <210> SEQ ID NO 332 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 332 ggacagttaa atttagccct 20 <210> SEQ ID NO 333 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 333 cggacagtta aatttagccc 20 <210> SEQ ID NO 334 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 334 tcggacagtt aaatttagcc 20 <210> SEQ ID NO 335 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 335 ctcggacagt taaatttagc 20 <210> SEQ ID NO 336 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 336 actcggacag ttaaatttag 20 <210> SEQ ID NO 337 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 337 gactcggaca gttaaattta 20 <210> SEQ ID NO 338 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 338 cgactcggac agttaaattt 20 <210> SEQ ID NO 339 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 339 ccgactcgga cagttaaatt 20 <210> SEQ ID NO 340 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 340 tccgactcgg acagttaaat 20 <210> SEQ ID NO 341 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 341 tctcctcacg gaagca 16 <210> SEQ ID NO 342 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 342 ctctcctcac ggaagc 16 <210> SEQ ID NO 343 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 343 cctctcctca cggaag 16 <210> SEQ ID NO 344 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 344 aaacttgctc agcagt 16 <210> SEQ ID NO 345 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 345 caaacttgct cagcag 16 <210> SEQ ID NO 346 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 346 ccaaacttgc tcagca 16 <210> SEQ ID NO 347 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 347 cccaaacttg ctcagc 16 <210> SEQ ID NO 348 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 348 ccccaaactt gctcag 16 <210> SEQ ID NO 349 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 349 tccccaaact tgctca 16 <210> SEQ ID NO 350 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 350 gtttgatgtc cctgtg 16 <210> SEQ ID NO 351 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 351 ggtttgatgt ccctgt 16 <210> SEQ ID NO 352 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 352 gggtttgatg tccctg 16 <210> SEQ ID NO 353 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 353 acagcctgca ggatct 16 <210> SEQ ID NO 354 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 354 cacagcctgc aggatc 16 <210> SEQ ID NO 355 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 355 ccacagcctg caggat 16 <210> SEQ ID NO 356 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 356 cccacagcct gcagga 16 <210> SEQ ID NO 357 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 357 gcccacagcc tgcagg 16 <210> SEQ ID NO 358 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 358 cgcccacagc ctgcag 16 <210> SEQ ID NO 359 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 359 ccgcccacag cctgca 16 <210> SEQ ID NO 360 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 360 accgcccaca gcctgc 16 <210> SEQ ID NO 361 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 361 caccgcccac agcctg 16 <210> SEQ ID NO 362 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 362 ccaccgccca cagcct 16 <210> SEQ ID NO 363 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 363 cccaccgccc acagcc 16 <210> SEQ ID NO 364 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 364 gcccaccgcc cacagc 16 <210> SEQ ID NO 365 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 365 ccaggcccac cgccca 16 <210> SEQ ID NO 366 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 366 cccaggccca ccgccc 16 <210> SEQ ID NO 367 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 367 tcccaggccc accgcc 16 <210> SEQ ID NO 368 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 368 tgcctgtccc aggccc 16 <210> SEQ ID NO 369 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 369 ctgcctgtcc caggcc 16 <210> SEQ ID NO 370 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 370 gctgcctgtc ccaggc 16 <210> SEQ ID NO 371 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 371 ggtggcacct tcgaaa 16 <210> SEQ ID NO 372 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 372 cggtggcacc ttcgaa 16 <210> SEQ ID NO 373 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 373 tcggtggcac cttcga 16 <210> SEQ ID NO 374 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 374 agtgagcccg tcctcc 16 <210> SEQ ID NO 375 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 375 cagtgagccc gtcctc 16 <210> SEQ ID NO 376 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 376 gcagtgagcc cgtcct 16 <210> SEQ ID NO 377 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 377 tcccgaatgt ccgaca 16 <210> SEQ ID NO 378 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 378 ttcccgaatg tccgac 16 <210> SEQ ID NO 379 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 379 cttcccgaat gtccga 16 <210> SEQ ID NO 380 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 380 ccttcccgaa tgtccg 16 <210> SEQ ID NO 381 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 381 accttcccga atgtcc 16 <210> SEQ ID NO 382 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 382 caccttcccg aatgtc 16 <210> SEQ ID NO 383 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 383 gcaccttccc gaatgt 16 <210> SEQ ID NO 384 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 384 cgcaccttcc cgaatg 16 <210> SEQ ID NO 385 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 385 atccgctcct gcaact 16 <210> SEQ ID NO 386 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 386 catccgctcc tgcaac 16 <210> SEQ ID NO 387 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 387 ccatccgctc ctgcaa 16 <210> SEQ ID NO 388 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 388 gctccctctg cctgca 16 <210> SEQ ID NO 389 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 389 aggtggatcc gtggcc 16 <210> SEQ ID NO 390 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 390 gggaaggtgg atccgt 16 <210> SEQ ID NO 391 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 391 acaggagcag ggaaag 16 <210> SEQ ID NO 392 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 392 cagactgcgg tgagtt 16 <210> SEQ ID NO 393 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 393 ggctcctggg cggcgc 16 <210> SEQ ID NO 394 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 394 ggcggctcct gggcgg 16 <210> SEQ ID NO 395 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 395 cgcgggcggc tcctgg 16 <210> SEQ ID NO 396 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 396 gagcgcgggc ggctcc 16 <210> SEQ ID NO 397 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 397 ggttcaggga gcgcgg 16 <210> SEQ ID NO 398 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 398 agttctaggg ttcagg 16 <210> SEQ ID NO 399 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 399 cagttctagg gttcag 16 <210> SEQ ID NO 400 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 400 acagttctag ggttca 16 <210> SEQ ID NO 401 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 401 gacagttcta gggttc 16 <210> SEQ ID NO 402 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 402 agacagttct agggtt 16 <210> SEQ ID NO 403 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 403 aagacagttc tagggt 16 <210> SEQ ID NO 404 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 404 gaagacagtt ctaggg 16 <210> SEQ ID NO 405 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 405 cgaagacagt tctagg 16 <210> SEQ ID NO 406 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 406 tcgaagacag ttctag 16 <210> SEQ ID NO 407 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 407 gtcgaagaca gttcta 16 <210> SEQ ID NO 408 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 408 agtcgaagac agttct 16 <210> SEQ ID NO 409 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 409 gagtcgaaga cagttc 16 <210> SEQ ID NO 410 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 410 ggagtcgaag acagtt 16 <210> SEQ ID NO 411 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 411 cggagtcgaa gacagt 16 <210> SEQ ID NO 412 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 412 ccggagtcga agacag 16 <210> SEQ ID NO 413 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 413 cccggagtcg aagaca 16 <210> SEQ ID NO 414 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 414 ccccggagtc gaagac 16 <210> SEQ ID NO 415 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 415 gccccggagt cgaaga 16 <210> SEQ ID NO 416 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 416 ggccccggag tcgaag 16 <210> SEQ ID NO 417 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 417 gggccccgga gtcgaa 16 <210> SEQ ID NO 418 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 418 cggttgtgaa ctggca 16 <210> SEQ ID NO 419 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 419 agcggttgtg aactgg 16 <210> SEQ ID NO 420 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 420 gcactttgcg aaccaa 16 <210> SEQ ID NO 421 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 421 tgcactttgc gaacca 16 <210> SEQ ID NO 422 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 422 actttgcgaa ccaacg 16 <210> SEQ ID NO 423 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 423 aaagctttgc actttg 16 <210> SEQ ID NO 424 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 424 tcccgagtaa gcaggc 16 <210> SEQ ID NO 425 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 425 gcagcgcaag tgagga 16 <210> SEQ ID NO 426 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 426 gtcagcgagt cggagg 16 <210> SEQ ID NO 427 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 427 cctgtcagcg agtcgg 16 <210> SEQ ID NO 428 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 428 gcctgtcagc gagtcg 16 <210> SEQ ID NO 429 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 429 agcctgtcag cgagtc 16 <210> SEQ ID NO 430 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 430 gggtctcagt gcatcc 16 <210> SEQ ID NO 431 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 431 aggtttttcc agaggc 16 <210> SEQ ID NO 432 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 432 aaggtttttc cagagg 16 <210> SEQ ID NO 433 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 433 ggtcactgct gggtcc 16 <210> SEQ ID NO 434 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 434 gtggtttctg tctgct 16 <210> SEQ ID NO 435 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 435 cgtggtttct gtctgc 16 <210> SEQ ID NO 436 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 436 gaactggcag gcggtg 16 <210> SEQ ID NO 437 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 437 tgtgaactgg caggcg 16 <210> SEQ ID NO 438 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 438 ggttgtgaac tggcag 16 <210> SEQ ID NO 439 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 439 gagcggttgt gaactg 16 <210> SEQ ID NO 440 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 440 actggagctg ggcgga 16 <210> SEQ ID NO 441 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 441 aggactggag ctgggc 16 <210> SEQ ID NO 442 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 442 tcacaggact ggagct 16 <210> SEQ ID NO 443 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 443 atcacaggac tggagc 16 <210> SEQ ID NO 444 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 444 ggatcacagg actgga 16 <210> SEQ ID NO 445 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 445 cagcctggcc gaaaga 16 <210> SEQ ID NO 446 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 446 ctcagcctgg ccgaaa 16 <210> SEQ ID NO 447 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 447 gtcagggcct cagcct 16 <210> SEQ ID NO 448 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 448 cgtcagggcc tcagcc 16 <210> SEQ ID NO 449 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 449 tttgcacttt gcgaac 16 <210> SEQ ID NO 450 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 450 gaaagctttg cacttt 16 <210> SEQ ID NO 451 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 451 aatttcccga gtaagc 16 <210> SEQ ID NO 452 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 452 gcaaatttcc cgagta 16 <210> SEQ ID NO 453 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 453 agcaaatttc ccgagt 16 <210> SEQ ID NO 454 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 454 aagcaaattt cccgag 16 <210> SEQ ID NO 455 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 455 aaagcaaatt tcccga 16 <210> SEQ ID NO 456 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 456 aaaagcaaat ttcccg 16 <210> SEQ ID NO 457 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 457 gcaaaagcaa atttcc 16 <210> SEQ ID NO 458 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 458 ggcaaaagca aatttc 16 <210> SEQ ID NO 459 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 459 ttggcaaaag caaatt 16 <210> SEQ ID NO 460 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 460 gtttggcaaa agcaaa 16 <210> SEQ ID NO 461 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 461 ggtttggcaa aagcaa 16 <210> SEQ ID NO 462 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 462 gggtttggca aaagca 16 <210> SEQ ID NO 463 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 463 gcgggtttgg caaaag 16 <210> SEQ ID NO 464 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 464 agcgggtttg gcaaaa 16 <210> SEQ ID NO 465 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 465 aagcgggttt ggcaaa 16 <210> SEQ ID NO 466 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 466 ctccgagagc agcgca 16 <210> SEQ ID NO 467 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 467 gctccgagag cagcgc 16 <210> SEQ ID NO 468 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 468 ggctccgaga gcagcg 16 <210> SEQ ID NO 469 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 469 taaatatcca aaccgc 16 <210> SEQ ID NO 470 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 470 gtcaataaat atccaa 16 <210> SEQ ID NO 471 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 471 aggtcaataa atatcc 16 <210> SEQ ID NO 472 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 472 cgaggtcaat aaatat 16 <210> SEQ ID NO 473 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 473 acgaggtcaa taaata 16 <210> SEQ ID NO 474 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 474 gacgaggtca ataaat 16 <210> SEQ ID NO 475 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 475 ggacgaggtc aataaa 16 <210> SEQ ID NO 476 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 476 aggacgaggt caataa 16 <210> SEQ ID NO 477 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 477 gaggacgagg tcaata 16 <210> SEQ ID NO 478 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 478 ggaggacgag gtcaat 16 <210> SEQ ID NO 479 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 479 cggaggacga ggtcaa 16 <210> SEQ ID NO 480 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 480 tcggaggacg aggtca 16 <210> SEQ ID NO 481 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 481 gtcggaggac gaggtc 16 <210> SEQ ID NO 482 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 482 agtcggagga cgaggt 16 <210> SEQ ID NO 483 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 483 gagtcggagg acgagg 16 <210> SEQ ID NO 484 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 484 cgagtcggag gacgag 16 <210> SEQ ID NO 485 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 485 agcgagtcgg aggacg 16 <210> SEQ ID NO 486 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 486 cagcgagtcg gaggac 16 <210> SEQ ID NO 487 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 487 tcagcgagtc ggagga 16 <210> SEQ ID NO 488 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 488 tgtcagcgag tcggag 16 <210> SEQ ID NO 489 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 489 ctgtcagcga gtcgga 16 <210> SEQ ID NO 490 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 490 cagtgcatcc aaaacg 16 <210> SEQ ID NO 491 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 491 tcagtgcatc caaaac 16 <210> SEQ ID NO 492 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 492 ctcagtgcat ccaaaa 16 <210> SEQ ID NO 493 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 493 tctcagtgca tccaaa 16 <210> SEQ ID NO 494 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 494 gtctcagtgc atccaa 16 <210> SEQ ID NO 495 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 495 caataaatac cgagga 16 <210> SEQ ID NO 496 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 496 agacaataaa taccga 16 <210> SEQ ID NO 497 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 497 cagacaataa ataccg 16 <210> SEQ ID NO 498 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 498 cacggaagca cgacac 16 <210> SEQ ID NO 499 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 499 tcacggaagc acgaca 16 <210> SEQ ID NO 500 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 500 ctcacggaag cacgac 16 <210> SEQ ID NO 501 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 501 gtccctctcc tcacgg 16 <210> SEQ ID NO 502 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 502 cgtccctctc ctcacg 16 <210> SEQ ID NO 503 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 503 cccattcacc aacacg 16 <210> SEQ ID NO 504 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 504 ccccattcac caacac 16 <210> SEQ ID NO 505 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 505 tccccattca ccaaca 16 <210> SEQ ID NO 506 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 506 gtccccattc accaac 16 <210> SEQ ID NO 507 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 507 ggtccccatt caccaa 16 <210> SEQ ID NO 508 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 508 cggtccccat tcacca 16 <210> SEQ ID NO 509 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 509 ccggtcccca ttcacc 16 <210> SEQ ID NO 510 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 510 gccggtcccc attcac 16 <210> SEQ ID NO 511 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 511 cgccggtccc cattca 16 <210> SEQ ID NO 512 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 512 ccgccggtcc ccattc 16 <210> SEQ ID NO 513 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 513 accgccggtc cccatt 16 <210> SEQ ID NO 514 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 514 caccgccggt ccccat 16 <210> SEQ ID NO 515 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 515 ccaccgccgg tcccca 16 <210> SEQ ID NO 516 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 516 tccaccgccg gtcccc 16 <210> SEQ ID NO 517 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 517 atccaccgcc ggtccc 16 <210> SEQ ID NO 518 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 518 gatccaccgc cggtcc 16 <210> SEQ ID NO 519 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 519 tgatccaccg ccggtc 16 <210> SEQ ID NO 520 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 520 gtgatccacc gccggt 16 <210> SEQ ID NO 521 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 521 cgtgatccac cgccgg 16 <210> SEQ ID NO 522 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 522 catcctggaa ggcgaa 16 <210> SEQ ID NO 523 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 523 tcatcctgga aggcga 16 <210> SEQ ID NO 524 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 524 agttctcatc ctggaa 16 <210> SEQ ID NO 525 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 525 tagttctcat cctgga 16 <210> SEQ ID NO 526 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 526 gtagttctca tcctgg 16 <210> SEQ ID NO 527 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 527 caggtacagg tagttc 16 <210> SEQ ID NO 528 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 528 ccaggtacag gtagtt 16 <210> SEQ ID NO 529 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 529 gaccaggtac aggtag 16 <210> SEQ ID NO 530 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 530 ctccatgacc aggtac 16 <210> SEQ ID NO 531 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 531 actccatgac caggta 16 <210> SEQ ID NO 532 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 532 tactccatga ccaggt 16 <210> SEQ ID NO 533 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 533 atactccatg accagg 16 <210> SEQ ID NO 534 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 534 aatactccat gaccag 16 <210> SEQ ID NO 535 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 535 taatactcca tgacca 16 <210> SEQ ID NO 536 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 536 gtaatactcc atgacc 16 <210> SEQ ID NO 537 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 537 cgtaatactc catgac 16 <210> SEQ ID NO 538 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 538 cttgctcagc agtgtc 16 <210> SEQ ID NO 539 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 539 acttgctcag cagtgt 16 <210> SEQ ID NO 540 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 540 aacttgctca gcagtg 16 <210> SEQ ID NO 541 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 541 gctccccaaa cttgct 16 <210> SEQ ID NO 542 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 542 cgctccccaa acttgc 16 <210> SEQ ID NO 543 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 543 ccgctcccca aacttg 16 <210> SEQ ID NO 544 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 544 tccgctcccc aaactt 16 <210> SEQ ID NO 545 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 545 atccgctccc caaact 16 <210> SEQ ID NO 546 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 546 aatccgctcc ccaaac 16 <210> SEQ ID NO 547 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 547 gaatccgctc cccaaa 16 <210> SEQ ID NO 548 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 548 ggaatccgct ccccaa 16 <210> SEQ ID NO 549 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 549 cggaatccgc tcccca 16 <210> SEQ ID NO 550 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 550 ccggaatccg ctcccc 16 <210> SEQ ID NO 551 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 551 gccggaatcc gctccc 16 <210> SEQ ID NO 552 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 552 agaagcgcgc catctc 16 <210> SEQ ID NO 553 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 553 tagaagcgcg ccatct 16 <210> SEQ ID NO 554 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 554 gtagaagcgc gccatc 16 <210> SEQ ID NO 555 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 555 ggtagaagcg cgccat 16 <210> SEQ ID NO 556 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 556 aggtagaagc gcgcca 16 <210> SEQ ID NO 557 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 557 caggtagaag cgcgcc 16 <210> SEQ ID NO 558 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 558 ccaggtagaa gcgcgc 16 <210> SEQ ID NO 559 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 559 gccaggtaga agcgcg 16 <210> SEQ ID NO 560 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 560 cgccaggtag aagcgc 16 <210> SEQ ID NO 561 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 561 ccgccaggta gaagcg 16 <210> SEQ ID NO 562 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 562 tccgccaggt agaagc 16 <210> SEQ ID NO 563 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 563 tgacaatctc cgccag 16 <210> SEQ ID NO 564 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 564 atgacaatct ccgcca 16 <210> SEQ ID NO 565 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 565 catgacaatc tccgcc 16 <210> SEQ ID NO 566 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 566 ccatgacaat ctccgc 16 <210> SEQ ID NO 567 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 567 gccatgacaa tctccg 16 <210> SEQ ID NO 568 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 568 ggccatgaca atctcc 16 <210> SEQ ID NO 569 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 569 ccaactgttc tcttag 16 <210> SEQ ID NO 570 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 570 aaccaactgt tctctt 16 <210> SEQ ID NO 571 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 571 ccagtaataa aagctg 16 <210> SEQ ID NO 572 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 572 gtccagtaat aaaagc 16 <210> SEQ ID NO 573 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 573 gttgtccagt aataaa 16 <210> SEQ ID NO 574 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 574 atgttgtcca gtaata 16 <210> SEQ ID NO 575 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 575 taatgttgtc cagtaa 16 <210> SEQ ID NO 576 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 576 ttcaatcctg acccac 16 <210> SEQ ID NO 577 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 577 ggttcaatcc tgaccc 16 <210> SEQ ID NO 578 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 578 tgggttcaat cctgac 16 <210> SEQ ID NO 579 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 579 gatgggttca atcctg 16 <210> SEQ ID NO 580 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 580 aggatgggtt caatcc 16 <210> SEQ ID NO 581 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 581 agaggatggg ttcaat 16 <210> SEQ ID NO 582 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 582 atagaggatg ggttca 16 <210> SEQ ID NO 583 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 583 ccctcctgtg ggaaca 16 <210> SEQ ID NO 584 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 584 gtccctcctg tgggaa 16 <210> SEQ ID NO 585 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 585 cagtccctcc tgtggg 16 <210> SEQ ID NO 586 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 586 agcagtccct cctgtg 16 <210> SEQ ID NO 587 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 587 actcagctgt gggaag 16 <210> SEQ ID NO 588 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 588 cccactcagc tgtggg 16 <210> SEQ ID NO 589 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 589 accccactca gctgtg 16 <210> SEQ ID NO 590 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 590 acaccccact cagctg 16 <210> SEQ ID NO 591 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 591 gcacacccca ctcagc 16 <210> SEQ ID NO 592 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 592 tcagcacacc ccactc 16 <210> SEQ ID NO 593 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 593 gtggtcctaa gactgg 16 <210> SEQ ID NO 594 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 594 gatgtggtcc taagac 16 <210> SEQ ID NO 595 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 595 cagatgtggt cctaag 16 <210> SEQ ID NO 596 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 596 cctccacaga tgtggt 16 <210> SEQ ID NO 597 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 597 cacctccaca gatgtg 16 <210> SEQ ID NO 598 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 598 ggccacctcc acagat 16 <210> SEQ ID NO 599 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 599 tgcttggctc tggcca 16 <210> SEQ ID NO 600 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 600 actgcttggc tctggc 16 <210> SEQ ID NO 601 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 601 agactgcttg gctctg 16 <210> SEQ ID NO 602 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 602 ggagactgct tggctc 16 <210> SEQ ID NO 603 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 603 tgcagacccc tcttct 16 <210> SEQ ID NO 604 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 604 ctcctccctt gacatg 16 <210> SEQ ID NO 605 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 605 ccagaccccc atgttc 16 <210> SEQ ID NO 606 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 606 gtccagaccc ccatgt 16 <210> SEQ ID NO 607 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 607 gggtccagac ccccat 16 <210> SEQ ID NO 608 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 608 accttctgca gggact 16 <210> SEQ ID NO 609 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 609 taaaccttct gcaggg 16 <210> SEQ ID NO 610 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 610 gaaaagccct gcccct 16 <210> SEQ ID NO 611 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 611 taggaaaagc cctgcc 16 <210> SEQ ID NO 612 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 612 cttaggaaaa gccctg 16 <210> SEQ ID NO 613 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 613 tgcttaggaa aagccc 16 <210> SEQ ID NO 614 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 614 tctgcttagg aaaagc 16 <210> SEQ ID NO 615 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 615 ctcctctgct taggaa 16 <210> SEQ ID NO 616 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 616 ccctcctctg cttagg 16 <210> SEQ ID NO 617 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 617 ctgatttgag gaaggg 16 <210> SEQ ID NO 618 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 618 tcctgatttg aggaag 16 <210> SEQ ID NO 619 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 619 cctcctgatt tgagga 16 <210> SEQ ID NO 620 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 620 gacctcctga tttgag 16 <210> SEQ ID NO 621 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 621 aagacctcct gatttg 16 <210> SEQ ID NO 622 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 622 ccaagacctc ctgatt 16 <210> SEQ ID NO 623 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 623 ctgcttccaa gacctc 16 <210> SEQ ID NO 624 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 624 agctgcttcc aagacc 16 <210> SEQ ID NO 625 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 625 gcagctgctt ccaaga 16 <210> SEQ ID NO 626 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 626 ctggtggaga accaga 16 <210> SEQ ID NO 627 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 627 ctctggtgga gaacca 16 <210> SEQ ID NO 628 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 628 ttctctggtg gagaac 16 <210> SEQ ID NO 629 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 629 gattctctgg tggaga 16 <210> SEQ ID NO 630 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 630 acttactgtt tcatcc 16 <210> SEQ ID NO 631 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 631 cggaccccct cccctc 16 <210> SEQ ID NO 632 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 632 gacggacccc ctcccc 16 <210> SEQ ID NO 633 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 633 ctgacggacc ccctcc 16 <210> SEQ ID NO 634 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 634 ccctgacgga ccccct 16 <210> SEQ ID NO 635 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 635 aagccctcac cttttc 16 <210> SEQ ID NO 636 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 636 ggaagccctc accttt 16 <210> SEQ ID NO 637 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 637 cgggaagccc tcacct 16 <210> SEQ ID NO 638 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 638 cccgggaagc cctcac 16 <210> SEQ ID NO 639 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 639 cacccgggaa gccctc 16 <210> SEQ ID NO 640 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 640 gccacccggg aagccc 16 <210> SEQ ID NO 641 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 641 acgccacccg ggaagc 16 <210> SEQ ID NO 642 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 642 ctgttcagga agtccc 16 <210> SEQ ID NO 643 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 643 ttctgttcag gaagtc 16 <210> SEQ ID NO 644 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 644 gcttctgttc aggaag 16 <210> SEQ ID NO 645 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 645 gcttgggccc acccct 16 <210> SEQ ID NO 646 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 646 aggcttgggc ccaccc 16 <210> SEQ ID NO 647 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 647 cgaggcttgg gcccac 16 <210> SEQ ID NO 648 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 648 agcgaggctt gggccc 16 <210> SEQ ID NO 649 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 649 agagcgaggc ttgggc 16 <210> SEQ ID NO 650 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 650 gcagagcgag gcttgg 16 <210> SEQ ID NO 651 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 651 gagcagagcg aggctt 16 <210> SEQ ID NO 652 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 652 aaaggagcag agcgag 16 <210> SEQ ID NO 653 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 653 caaaaggagc agagcg 16 <210> SEQ ID NO 654 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 654 tggaccaaaa ggagca 16 <210> SEQ ID NO 655 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 655 cctggaccaa aaggag 16 <210> SEQ ID NO 656 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 656 cacctggacc aaaagg 16 <210> SEQ ID NO 657 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 657 cgcacctgga ccaaaa 16 <210> SEQ ID NO 658 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 658 gaccgcacct ggacca 16 <210> SEQ ID NO 659 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 659 accttgtagt ggacga 16 <210> SEQ ID NO 660 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 660 tcaccttgta gtggac 16 <210> SEQ ID NO 661 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 661 gctcaccttg tagtgg 16 <210> SEQ ID NO 662 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 662 ggagaggagg cgatag 16 <210> SEQ ID NO 663 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 663 agggagagga ggcgat 16 <210> SEQ ID NO 664 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 664 ctcctgctca gaggga 16 <210> SEQ ID NO 665 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 665 gtgctcctgc tcagag 16 <210> SEQ ID NO 666 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 666 aggtgctcct gctcag 16 <210> SEQ ID NO 667 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 667 agaggtgctc ctgctc 16 <210> SEQ ID NO 668 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 668 agagaggtgc tcctgc 16 <210> SEQ ID NO 669 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 669 accccgcccc cgctca 16 <210> SEQ ID NO 670 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 670 ctaccccgcc cccgct 16 <210> SEQ ID NO 671 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 671 acctaccccg cccccg 16 <210> SEQ ID NO 672 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 672 gtacctaccc cgcccc 16 <210> SEQ ID NO 673 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 673 aggtacctac cccgcc 16 <210> SEQ ID NO 674 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 674 gggaggttcc cgcagc 16 <210> SEQ ID NO 675 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 675 gtccttactc caactt 16 <210> SEQ ID NO 676 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 676 ctgtccttac tccaac 16 <210> SEQ ID NO 677 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 677 cactgtcctt actcca 16 <210> SEQ ID NO 678 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 678 ggcactgtcc ttactc 16 <210> SEQ ID NO 679 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 679 taggcactgt ccttac 16 <210> SEQ ID NO 680 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 680 ggtaggcact gtcctt 16 <210> SEQ ID NO 681 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 681 gtcactgctg ggtcct 16 <210> SEQ ID NO 682 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 682 aggtcactgc tgggtc 16 <210> SEQ ID NO 683 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 683 ctaggtcact gctggg 16 <210> SEQ ID NO 684 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 684 gtctaggtca ctgctg 16 <210> SEQ ID NO 685 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 685 aagtctaggt cactgc 16 <210> SEQ ID NO 686 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 686 gcactccatt gtctca 16 <210> SEQ ID NO 687 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 687 ctgcactcca ttgtct 16 <210> SEQ ID NO 688 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 688 ccctgcactc cattgt 16 <210> SEQ ID NO 689 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 689 ccccctgcac tccatt 16 <210> SEQ ID NO 690 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 690 cttgctgagt caggag 16 <210> SEQ ID NO 691 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 691 tccttgctga gtcagg 16 <210> SEQ ID NO 692 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 692 cttccttgct gagtca 16 <210> SEQ ID NO 693 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 693 accttccttg ctgagt 16 <210> SEQ ID NO 694 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 694 ggaccttcct tgctga 16 <210> SEQ ID NO 695 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 695 caggaccttc cttgct 16 <210> SEQ ID NO 696 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 696 agccctccag gacctt 16 <210> SEQ ID NO 697 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 697 tagctcccca ctccag 16 <210> SEQ ID NO 698 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 698 gatagctccc cactcc 16 <210> SEQ ID NO 699 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 699 cagatagctc cccact 16 <210> SEQ ID NO 700 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 700 ctcagatagc tcccca 16 <210> SEQ ID NO 701 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 701 agctcagata gctccc 16 <210> SEQ ID NO 702 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 702 tcagctcaga tagctc 16 <210> SEQ ID NO 703 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 703 tctcagctca gatagc 16 <210> SEQ ID NO 704 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 704 gagtcctctc ctgctt 16 <210> SEQ ID NO 705 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 705 ggaggagtcc tctcct 16 <210> SEQ ID NO 706 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 706 gaggaggagt cctctc 16 <210> SEQ ID NO 707 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 707 caaaagggca cccaga 16 <210> SEQ ID NO 708 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 708 agcaaaaggg caccca 16 <210> SEQ ID NO 709 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 709 ggatccccag tattgt 16 <210> SEQ ID NO 710 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 710 ctggatcccc agtatt 16 <210> SEQ ID NO 711 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 711 tgctggatcc ccagta 16 <210> SEQ ID NO 712 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 712 attctctaga ctgcaa 16 <210> SEQ ID NO 713 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 713 taattctcta gactgc 16 <210> SEQ ID NO 714 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 714 tctaattctc tagact 16 <210> SEQ ID NO 715 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 715 tctctaattc tctaga 16 <210> SEQ ID NO 716 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 716 ctccataatt ctctaa 16 <210> SEQ ID NO 717 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 717 actctccata attctc 16 <210> SEQ ID NO 718 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 718 acactctcca taattc 16 <210> SEQ ID NO 719 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 719 ccacactctc cataat 16 <210> SEQ ID NO 720 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 720 tgccacactc tccata 16 <210> SEQ ID NO 721 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 721 cccatgccca tcctgc 16 <210> SEQ ID NO 722 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 722 ggacagagaa atgttg 16 <210> SEQ ID NO 723 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 723 ggcataggac agagaa 16 <210> SEQ ID NO 724 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 724 gtggcatagg acagag 16 <210> SEQ ID NO 725 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 725 tggtggcata ggacag 16 <210> SEQ ID NO 726 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 726 cttactctgc ccctcc 16 <210> SEQ ID NO 727 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 727 accttactct gcccct 16 <210> SEQ ID NO 728 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 728 tgaccttact ctgccc 16 <210> SEQ ID NO 729 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 729 gctgacctta ctctgc 16 <210> SEQ ID NO 730 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 730 ctgctgacct tactct 16 <210> SEQ ID NO 731 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 731 ctctgctgac cttact 16 <210> SEQ ID NO 732 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 732 gcctctgctg acctta 16 <210> SEQ ID NO 733 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 733 ccatggctct gagtca 16 <210> SEQ ID NO 734 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 734 agccatggct ctgagt 16 <210> SEQ ID NO 735 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 735 taagccatgg ctctga 16 <210> SEQ ID NO 736 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 736 tagcctgctg tgactc 16 <210> SEQ ID NO 737 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 737 atgggaggct gttggc 16 <210> SEQ ID NO 738 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 738 ccatgggagg ctgttg 16 <210> SEQ ID NO 739 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 739 ggccatggga ggctgt 16 <210> SEQ ID NO 740 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 740 gtgcagagag gccatg 16 <210> SEQ ID NO 741 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 741 gagctcccag catgac 16 <210> SEQ ID NO 742 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 742 agggagctcc cagcat 16 <210> SEQ ID NO 743 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 743 gccatagagc ccactt 16 <210> SEQ ID NO 744 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 744 gggccataga gcccac 16 <210> SEQ ID NO 745 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 745 atgctggccc tcctgg 16 <210> SEQ ID NO 746 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 746 agctgcccca tgctgg 16 <210> SEQ ID NO 747 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 747 cgcccctggc agctgc 16 <210> SEQ ID NO 748 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 748 tgcgcccctg gcagct 16 <210> SEQ ID NO 749 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 749 gctgcgcccc tggcag 16 <210> SEQ ID NO 750 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 750 cggctgcgcc cctggc 16 <210> SEQ ID NO 751 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 751 gtcggctgcg cccctg 16 <210> SEQ ID NO 752 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 752 ctgtcggctg cgcccc 16 <210> SEQ ID NO 753 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 753 gcctgtcggc tgcgcc 16 <210> SEQ ID NO 754 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 754 ctgcctgtcg gctgcg 16 <210> SEQ ID NO 755 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 755 acctgcctgt cggctg 16 <210> SEQ ID NO 756 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 756 acacctgcct gtcggc 16 <210> SEQ ID NO 757 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 757 gaacacctgc ctgtcg 16 <210> SEQ ID NO 758 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 758 ccgaacacct gcctgt 16 <210> SEQ ID NO 759 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 759 cgccgaacac ctgcct 16 <210> SEQ ID NO 760 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 760 cctgggcacc tgttgg 16 <210> SEQ ID NO 761 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 761 gtgcctgggc acctgt 16 <210> SEQ ID NO 762 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 762 cgccctccca gtgcct 16 <210> SEQ ID NO 763 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 763 accgccctcc cagtgc 16 <210> SEQ ID NO 764 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 764 tcaccgccct cccagt 16 <210> SEQ ID NO 765 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 765 agtcaccgcc ctccca 16 <210> SEQ ID NO 766 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 766 tgagtcaccg ccctcc 16 <210> SEQ ID NO 767 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 767 cgtgagtcac cgccct 16 <210> SEQ ID NO 768 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 768 caaagctggt tctccc 16 <210> SEQ ID NO 769 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 769 tgcaaagctg gttctc 16 <210> SEQ ID NO 770 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 770 tctgcaaagc tggttc 16 <210> SEQ ID NO 771 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 771 tgtctgcaaa gctggt 16 <210> SEQ ID NO 772 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 772 cctgtctgca aagctg 16 <210> SEQ ID NO 773 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 773 cgcctgtctg caaagc 16 <210> SEQ ID NO 774 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 774 ttgtccctcc tggatc 16 <210> SEQ ID NO 775 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 775 agttgtccct cctgga 16 <210> SEQ ID NO 776 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 776 aaagttgtcc ctcctg 16 <210> SEQ ID NO 777 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 777 ccaaagttgt ccctcc 16 <210> SEQ ID NO 778 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 778 acccaaagtt gtccct 16 <210> SEQ ID NO 779 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 779 gaacccaaag ttgtcc 16 <210> SEQ ID NO 780 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 780 gaagaaccca aagttg 16 <210> SEQ ID NO 781 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 781 ccagaagaac ccaaag 16 <210> SEQ ID NO 782 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 782 cacccagaag aaccca 16 <210> SEQ ID NO 783 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 783 gcagaaccta caaaag 16 <210> SEQ ID NO 784 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 784 gtgcagaacc tacaaa 16 <210> SEQ ID NO 785 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 785 gggtgcagaa cctaca 16 <210> SEQ ID NO 786 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 786 gtgggtgcag aaccta 16 <210> SEQ ID NO 787 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 787 ccacacggct catagg 16 <210> SEQ ID NO 788 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 788 acccacacgg ctcata 16 <210> SEQ ID NO 789 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 789 tgacccacac ggctca 16 <210> SEQ ID NO 790 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 790 gctgacccac acggct 16 <210> SEQ ID NO 791 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 791 tggctgaccc acacgg 16 <210> SEQ ID NO 792 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 792 ggtggctgac ccacac 16 <210> SEQ ID NO 793 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 793 atggtggctg acccac 16 <210> SEQ ID NO 794 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 794 gaatggtggc tgaccc 16 <210> SEQ ID NO 795 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 795 ctaaaggacg caggga 16 <210> SEQ ID NO 796 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 796 aactaaagga cgcagg 16 <210> SEQ ID NO 797 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 797 gagaactaaa ggacgc 16 <210> SEQ ID NO 798 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 798 attggtccca agcccc 16 <210> SEQ ID NO 799 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 799 ccattggtcc caagcc 16 <210> SEQ ID NO 800 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 800 gcccattggt cccaag 16 <210> SEQ ID NO 801 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 801 acgcccattg gtccca 16 <210> SEQ ID NO 802 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 802 ccacgcccat tggtcc 16 <210> SEQ ID NO 803 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 803 caccacgccc attggt 16 <210> SEQ ID NO 804 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 804 agacccaact ccaccc 16 <210> SEQ ID NO 805 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 805 tcacctcgcc cctctt 16 <210> SEQ ID NO 806 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 806 cctcacctcg cccctc 16 <210> SEQ ID NO 807 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 807 agcccctcac ctcgcc 16 <210> SEQ ID NO 808 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 808 ctcaaagccc cccacg 16 <210> SEQ ID NO 809 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 809 atcctcaaag cccccc 16 <210> SEQ ID NO 810 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 810 ggatcctcaa agcccc 16 <210> SEQ ID NO 811 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 811 gcggatcctc aaagcc 16 <210> SEQ ID NO 812 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 812 gcgcggatcc tcaaag 16 <210> SEQ ID NO 813 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 813 gggcgcggat cctcaa 16 <210> SEQ ID NO 814 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 814 gagctgcagc cggaga 16 <210> SEQ ID NO 815 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 815 aggagctgca gccgga 16 <210> SEQ ID NO 816 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 816 cggaggagct gcagcc 16 <210> SEQ ID NO 817 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 817 acccggagga gctgca 16 <210> SEQ ID NO 818 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 818 gcacccggag gagctg 16 <210> SEQ ID NO 819 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 819 gggcacccgg aggagc 16 <210> SEQ ID NO 820 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 820 cagggcaccc ggagga 16 <210> SEQ ID NO 821 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 821 tgcagggcac ccggag 16 <210> SEQ ID NO 822 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 822 cctgcagggc acccgg 16 <210> SEQ ID NO 823 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 823 cgacacctgc agggca 16 <210> SEQ ID NO 824 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 824 cacgacacct gcaggg 16 <210> SEQ ID NO 825 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 825 agcacgacac ctgcag 16 <210> SEQ ID NO 826 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 826 gaagcacgac acctgc 16 <210> SEQ ID NO 827 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 827 ccaggtagtt ctcatc 16 <210> SEQ ID NO 828 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 828 caccaggtag ttctca 16 <210> SEQ ID NO 829 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 829 ctcaccaggt agttct 16 <210> SEQ ID NO 830 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 830 agctcaccag gtagtt 16 <210> SEQ ID NO 831 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 831 ggagctcacc aggtag 16 <210> SEQ ID NO 832 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 832 ccggagctca ccaggt 16 <210> SEQ ID NO 833 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 833 gcccggagct caccag 16 <210> SEQ ID NO 834 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 834 tagagcttcc tctccc 16 <210> SEQ ID NO 835 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 835 cctagagctt cctctc 16 <210> SEQ ID NO 836 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 836 atcctagagc ttcctc 16 <210> SEQ ID NO 837 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 837 caatcctaga gcttcc 16 <210> SEQ ID NO 838 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 838 cccaatccta gagctt 16 <210> SEQ ID NO 839 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 839 cccccaatcc tagagc 16 <210> SEQ ID NO 840 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 840 cacccccaat cctaga 16 <210> SEQ ID NO 841 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 841 agcaccccca atccta 16 <210> SEQ ID NO 842 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 842 gcagcacccc caatcc 16 <210> SEQ ID NO 843 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 843 gggcagcacc cccaat 16 <210> SEQ ID NO 844 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 844 tgacacaccc tcttac 16 <210> SEQ ID NO 845 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 845 cctgacacac cctctt 16 <210> SEQ ID NO 846 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 846 cacctgacac accctc 16 <210> SEQ ID NO 847 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 847 tccacctgac acaccc 16 <210> SEQ ID NO 848 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 848 catccacctg acacac 16 <210> SEQ ID NO 849 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 849 ctcatccacc tgacac 16 <210> SEQ ID NO 850 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 850 ccctcatcca cctgac 16 <210> SEQ ID NO 851 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 851 gcccctcatc cacctg 16 <210> SEQ ID NO 852 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 852 aggcccctca tccacc 16 <210> SEQ ID NO 853 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 853 gaaggcccct catcca 16 <210> SEQ ID NO 854 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 854 aggtaagaga cccccc 16 <210> SEQ ID NO 855 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 855 ccaggtaaga gacccc 16 <210> SEQ ID NO 856 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 856 ttccaggtaa gagacc 16 <210> SEQ ID NO 857 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 857 ccattccagg taagag 16 <210> SEQ ID NO 858 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 858 tcccattcca ggtaag 16 <210> SEQ ID NO 859 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 859 tatcccattc caggta 16 <210> SEQ ID NO 860 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 860 cctatcccat tccagg 16 <210> SEQ ID NO 861 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 861 gacctatccc attcca 16 <210> SEQ ID NO 862 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 862 aagacctatc ccattc 16 <210> SEQ ID NO 863 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 863 tgaagaccta tcccat 16 <210> SEQ ID NO 864 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 864 tggccccgtt agaatt 16 <210> SEQ ID NO 865 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 865 agtggccccg ttagaa 16 <210> SEQ ID NO 866 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 866 gcagtggccc cgttag 16 <210> SEQ ID NO 867 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 867 aggcagtggc cccgtt 16 <210> SEQ ID NO 868 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 868 ctaggcagtg gccccg 16 <210> SEQ ID NO 869 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 869 ccctaggcag tggccc 16 <210> SEQ ID NO 870 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 870 aggtcccaga cactcc 16 <210> SEQ ID NO 871 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 871 ataggtccca gacact 16 <210> SEQ ID NO 872 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 872 gaataggtcc cagaca 16 <210> SEQ ID NO 873 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 873 gagaataggt cccaga 16 <210> SEQ ID NO 874 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 874 cagagaatag gtccca 16

1 SEQUENCE LISTING <160> NUMBER OF SEQ ID NOS: 874 <210> SEQ ID NO 1 <211> LENGTH: 2877 <212> TYPE: DNA <213> ORGANISM: homo sapiens <400> SEQUENCE: 1 aggggggctg gaccaagggg tggggagaag gggaggaggc ctcggccggc cgcagagaga 60 agtggccaga gaggcccagg ggacagccag ggacaggcag acatgcagcc agggctccag 120 ggcctggaca ggggctgcca ggccctgtga caggaggacc ccgagccccc ggcccgggga 180 ggggccatgg tgctgcctgt ccaacatgtc agccgaggtg cggctgaggc ggctccagca 240 gctggtgttg gacccgggct tcctggggct ggagcccctg ctcgaccttc tcctgggcgt 300 ccaccaggag ctgggcgcct ccgaactggc ccaggacaag tacgtggccg acttcttgca 360 gtgggcggag cccatcgtgg tgaggcttaa ggaggtccga ctgcagaggg acgacttcga 420 gattctgaag gtgatcggac gcggggcgtt cagcgaggta gcggtagtga agatgaagca 480 gacgggccag gtgtatgcca tgaagatcat gaacaagtgg gacatgctga agaggggcga 540 ggtgtcgtgc ttccgtgagg agagggacgt gttggtgaat ggggaccggc ggtggatcac 600 gcagctgcac ttcgccttcc aggatgagaa ctacctgtac ctggtcatgg agtattacgt 660 gggcggggac ctgctgacac tgctgagcaa gtttggggag cggattccgg ccgagatggc 720 gcgcttctac ctggcggaga ttgtcatggc catagactcg gtgcaccggc ttggctacgt 780 gcacagggac atcaaacccg acaacatcct gctggaccgc tgtggccaca tccgcctggc 840 cgacttcggc tcttgcctca agctgcgggc agatggaacg gtgcggtcgc tggtggctgt 900 gggcacccca gactacctgt cccccgagat cctgcaggct gtgggcggtg ggcctgggac 960 aggcagctac gggcccgagt gtgactggtg ggcgctgggt gtattcgcct atgaaatgtt 1020 ctatgggcag acgcccttct acgcggattc cacggcggag acctatggca agatcgtcca 1080 ctacaaggag cacctctctc tgccgctggt ggacgaaggg gtccctgagg aggctcgaga 1140 cttcattcag cggttgctgt gtcccccgga gacacggctg ggccggggtg gagcaggcga 1200 cttccggaca catcccttct tctttggcct cgactgggat ggtctccggg acagcgtgcc 1260 cccctttaca ccggatttcg aaggtgccac cgacacatgc aacttcgact tggtggagga 1320 cgggctcact gccatggaga cactgtcgga cattcgggaa ggtgcgccgc taggggtcca 1380 cctgcctttt gtgggctact cctactcctg catggccctc agggacagtg aggtcccagg 1440 ccccacaccc atggaactgg aggccgagca gctgcttgag ccacacgtgc aagcgcccag 1500 cctggagccc tcggtgtccc cacaggatga aacagctgaa gtggcagttc cagcggctgt 1560 ccctgcggca gaggctgagg ccgaggtgac gctgcgggag ctccaggaag ccctggagga 1620 ggaggtgctc acccggcaga gcctgagccg ggagatggag gccatccgca cggacaacca 1680 gaacttcgcc agtcaactac gcgaggcaga ggctcggaac cgggacctag aggcacacgt 1740 ccggcagttg caggagcgga tggagttgct gcaggcagag ggagccacag ctgtcacggg 1800 ggtccccagt ccccgggcca cggatccacc ttcccatcta gatggccccc cggccgtggc 1860 tgtgggccag tgcccgctgg tggggccagg ccccatgcac cgccgccacc tgctgctccc 1920 tgccagggtc cctaggcctg gcctatcgga ggcgctttcc ctgctcctgt tcgccgttgt 1980 tctgtctcgt gccgccgccc tgggctgcat tgggttggtg gcccacgccg gccaactcac 2040 cgcagtctgg cgccgcccag gagccgcccg cgctccctga accctagaac tgtcttcgac 2100 tccggggccc cgttggaaga ctgagtgccc ggggcacggc acagaagccg cgcccaccgc 2160 ctgccagttc acaaccgctc cgagcgtggg tctccgccca gctccagtcc tgtgatccgg 2220 gcccgccccc tagcggccgg ggagggaggg gccgggtccg cggccggcga acggggctcg 2280 aagggtcctt gtagccggga atgctgctgc tgctgctgct gctgctgctg ctgctgctgc 2340 tgctgctgct gctgctgctg ctggggggat cacagaccat ttctttcttt cggccaggct 2400 gaggccctga cgtggatggg caaactgcag gcctgggaag gcagcaagcc gggccgtccg 2460 tgttccatcc tccacgcacc cccacctatc gttggttcgc aaagtgcaaa gctttcttgt 2520 gcatgacgcc ctgctctggg gagcgtctgg cgcgatctct gcctgcttac tcgggaaatt 2580 tgcttttgcc aaacccgctt tttcggggat cccgcgcccc cctcctcact tgcgctgctc 2640 tcggagcccc agccggctcc gcccgcttcg gcggtttgga tatttattga cctcgtcctc 2700 cgactcgctg acaggctaca ggacccccaa caaccccaat ccacgttttg gatgcactga 2760 gaccccgaca ttcctcggta tttattgtct gtccccacct aggaccccca cccccgaccc 2820 tcgcgaataa aaggccctcc atctgcccaa aaaaaaaaaa aaaaaaaaaa aaaaaaa 2877 <210> SEQ ID NO 2 <211> LENGTH: 14411 <212> TYPE: DNA <213> ORGANISM: homo sapiens <400> SEQUENCE: 2 ctcccagccc agcgcctccc accccttttc atagcaggaa aagccggagc ccagggaggg 60 aacggacctg cgagtcacac aactggtgac ccacaccagc ggctggagca ggaccctctt 120 ggggagaaga gcatcctgcc cgcagccagg gcccctcatc aaagtcctcg gtgtttttta 180 aattatcaga actgcccagg accacgtttc ccaggccctg cccagctggg actcctcggt 240 ccttgcctcc tagtttctca ggcctggccc tctcaaggcc caggcacccc aggccggttg 300 gaggccccga cttccactct ggagaaccgt ccaccctgga aagaagagct cagattcctc 360 ttggctctcg gagccgcagg gagtgtgtct tcccgcgcca ccctccaccc cccgaaatgt 420 ttctgtttct aatcccagcc tgggcaggaa tgtggctccc cggccagggg ccaaggagct 480 attttggggt ctcgtttgcc cagggagggc ttggctccac cactttcctc ccccagcctt 540 tgggcagcag gtcacccctg ttcaggctct gagggtgccc cctcctggtc ctgtcctcac 600 caccccttcc ccacctcctg ggaaaaaaaa aaaaaaaaaa aaaaaaagct ggtataaagc 660 agagagcctg agggctaaat ttaactgtcc gagtcggaat ccatctctga gtcacccaag 720 aagctgccct ggcctcccgt ccccttccca ggcctcaacc cctttctccc acccagcccc 780 aacccccagc cctcaccccc tagcccccag ttctggagct tgtcgggagc aagggggtgg 840 ttgctactgg gtcactcagc ctcaattggc cctgtttcag caatgggcag gttcttcttg 900 aaattcatca cacctgtggc ttcctctgtg ctctaccttt ttattggggt gacagtgtga 960 cagctgagat tctccatgca ttccccctac tctagcactg aagggttctg aagggccctg 1020 gaaggaggga gcttgggggg ctggcttgtg aggggttaag gctgggaggc gggagggggg 1080 ctggaccaag gggtggggag aaggggagga ggcctcggcc ggccgcagag agaagtggcc 1140 agagaggccc aggggacagc cagggacagg cagacatgca gccagggctc cagggcctgg 1200 acaggggctg ccaggccctg tgacaggagg accccgagcc cccggcccgg ggaggggcca 1260 tggtgctgcc tgtccaacat gtcagccgag gtgcggctga ggcggctcca gcagctggtg 1320 ttggacccgg gcttcctggg gctggagccc ctgctcgacc ttctcctggg cgtccaccag 1380 gagctgggcg cctccgaact ggcccaggac aagtacgtgg ccgacttctt gcagtggggt 1440 gagtgcctac cctcggggct cctgcagatg gggtgggggt ggggcaggag acaggtctgg 1500 gcacagaggc ctggctgttg ggggggcagg atggcaggat gggcatgggg agatcctccc 1560 atcctggggc tcagagtgtg gacctgggcc ctggggcaac atttctctgt cctatgccac 1620 cactctggag gggcagagta aggtcagcag aggctagggt ggctgtgact cagagccatg 1680 gcttaggagt cacagcaggc taggctgcca acagcctccc atggcctctc tgcaccccgc 1740 ctcagggtca gggtcagggt catgctggga gctccctctc ctaggaccct ccccccaaaa 1800 gtgggctcta tggccctctc ccctggtttc ctgtggcctg gggcaagcca ggagggccag 1860 catggggcag ctgccagggg cgcagccgac aggcaggtgt tcggcgccag cctctccagc 1920 tgccccaaca ggtgcccagg cactgggagg gcggtgactc acgcgggccc tgtgggagaa 1980 ccagctttgc agacaggcgc caccagtgcc ccctcctctg cgatccagga gggacaactt 2040 tgggttcttc tgggtgtgtc tccttctttt gtaggttctg cacccacccc cacccccagc 2100 cccaaagtct cggttcctat gagccgtgtg ggtcagccac cattcccgcc accccgggtc 2160 cctgcgtcct ttagttctcc tggcccaggg cctccaacct tccagctgtc ccacaaaacc 2220 ccttcttgca agggctttcc agggcctggg gccagggctg gaaggaggat gcttccgctt 2280 ctgccagctg ccttgtctgc ccacctcctc cccaagccca ggactcgggc tcactggtca 2340 ctggtttctt tcattcccag caccctgccc ctctggccct catatgtctg gccctcagtg 2400 actggtgttt ggtttttggc ctgtgtgtaa caaactgtgt gtgacacttg tttcctgttt 2460 ctccgccttc ccctgcttcc tcttgtgtcc atctctttct gacccaggcc tggttccttt 2520 ccctcctcct cccatttcac agatgggaag gtggaggcca agaagggcca ggccattcag 2580 cctctggaaa aaccttctcc caacctccca cagcccctaa tgactctcct ggcctccctt 2640 tagtagagga tgaagttggg ttggcagggt aaactgagac cgggtggggt aggggtctgg 2700 cgctcccggg aggagcactc cttttgtggc ccgagctgca tctcgcggcc cctcccctgc 2760 caggcctggg gcgggggagg gggccagggt tcctgctgcc ttaaaagggc tcaatgtctt 2820 ggctctctcc tccctccccc gtcctcagcc ctggctggtt cgtccctgct ggcccactct 2880 cccggaaccc cccggaaccc ctctctttcc tccagaaccc actgtctcct ctccttccct 2940 cccctcccat acccatccct ctctccatcc tgcctccact tcttccaccc ccgggagtcc 3000 aggcctccct gtccccacag tccctgagcc acaagcctcc accccagctg gtcccccacc 3060 caggctgccc agtttaacat tcctagtcat aggaccttga cttctgagag gcctgattgt 3120 catctgtaaa taaggggtag gactaaagca ctcctcctgg aggactgaga gatgggctgg 3180 accggagcac ttgagtctgg gatatgtgac catgctacct ttgtctccct gtcctgttcc 3240 ttcccccagc cccaaatcca gggttttcca aagtgtggtt caagaaccac ctgcatctga 3300 atctagaggt actggataca accccacgtc tgggccgtta cccaggacat tctacatgag 3360 aacgtggggg tggggccctg gctgcacctg aactgtcacc tggagtcagg gtggaaggtg 3420 gaagaactgg gtcttatttc cttctcccct tgttctttag ggtctgtcct tctgcagact 3480 ccgttacccc accctaacca tcctgcacac ccttggagcc ctctgggcca atgccctgtc 3540 ccgcaaaggg cttctcaggc atctcacctc tatgggaggg catttttggc ccccagaacc 3600 ttacacggtg tttatgtggg gaagcccctg ggaagcagac agtcctaggg tgaagctgag 3660 aggcagagag aaggggagac agacagaggg tggggctttc ccccttgtct ccagtgccct 3720 ttctggtgac cctcggttct tttcccccac caccccccca gcggagccca tcgtggtgag 3780 gcttaaggag gtccgactgc agagggacga cttcgagatt ctgaaggtga tcggacgcgg 3840 ggcgttcagc gaggtaagcc gaaccgggcg ggagcctgac ttgactcgtg gtgggcgggg 3900 cataggggtt ggggcggggc cttagaaatt gatgaatgac cgagccttag aacctagggc 3960 tgggctggag gcggggcttg ggaccaatgg gcgtggtgtg gcaggtgggg cggggccacg 4020

gctgggtgca gaagcgggtg gagttgggtc tgggcgagcc cttttgtttt cccgccgtct 4080 ccactctgtc tcactatctc gacctcaggt agcggtagtg aagatgaagc agacgggcca 4140 ggtgtatgcc atgaagatca tgaacaagtg ggacatgctg aagaggggcg aggtgagggg 4200 ctgggcggac gtggggggct ttgaggatcc gcgccccgtc tccggctgca gctcctccgg 4260 gtgccctgca ggtgtcgtgc ttccgtgagg agagggacgt gttggtgaat ggggaccggc 4320 ggtggatcac gcagctgcac ttcgccttcc aggatgagaa ctacctggtg agctccgggc 4380 cggggtgact aggaagaggg acaagagccc gtgctgtcac tggacgagga ggtggggaga 4440 ggaagctcta ggattggggg tgctgcccgg aaacgtctgt gggaaagtct gtgtgcggta 4500 agagggtgtg tcaggtggat gaggggcctt ccctatctga gacggggatg gtgtccttca 4560 ctgcccgttt ctggggtgat ctgggggact cttataaaga tgtctctgtt gcggggggtc 4620 tcttacctgg aatgggatag gtcttcagga attctaacgg ggccactgcc tagggaagga 4680 gtgtctggga cctattctct gggtgttggg tggcctctgg gttctctttc ccagaacatc 4740 tcagggggag tgaatctgcc cagtgacatc ccaggaaagt ttttttgttt gtgttttttt 4800 ttgaggggcg ggggcggggg ccgcaggtgg tctctgattt ggcccggcag atctctatgg 4860 ttatctctgg gctggggctg caggtctctg cccaaggatg gggtgtctct gggaggggtt 4920 gtcccagcca tccgtgatgg atcagggcct caggggacta ccaaccaccc atgacgaacc 4980 ccttctcagt acctggtcat ggagtattac gtgggcgggg acctgctgac actgctgagc 5040 aagtttgggg agcggattcc ggccgagatg gcgcgcttct acctggcgga gattgtcatg 5100 gccatagact cggtgcaccg gcttggctac gtgcacaggt gggtgcagca tggccgaggg 5160 gatagcaagc ttgttccctg gccgggttct tggaaggtca gagcccagag aggccagggc 5220 ctggagaggg accttcttgg ttggggccca ccggggggtg cctgggagta ggggtcagaa 5280 ctgtagaagc cctacagggg cggaacccga ggaagtgggg tcccaggtgg cactgcccgg 5340 aggggcggag cctggtggga ccacagaagg gaggttcatt tatcccaccc ttctcttttc 5400 ctccgtgcag ggacatcaaa cccgacaaca tcctgctgga ccgctgtggc cacatccgcc 5460 tggccgactt cggctcttgc ctcaagctgc gggcagatgg aacggtgagc cagtgccctg 5520 gccacagagc aactggggct gctgatgagg gatggaaggc acagagtgtg ggagcgggac 5580 tggatttgga ggggaaaaga ggtggtgtga cccaggctta agtgtgcatc tgtgtggcgg 5640 agtattagac caggcagagg gaggggctaa gcatttgggg agtggttgga aggagggccc 5700 agagctggtg ggcccagagg ggtgggccca agcctcgctc tgctcctttt ggtccaggtg 5760 cggtcgctgg tggctgtggg caccccagac tacctgtccc ccgagatcct gcaggctgtg 5820 ggcggtgggc ctgggacagg cagctacggg cccgagtgtg actggtgggc gctgggtgta 5880 ttcgcctatg aaatgttcta tgggcagacg cccttctacg cggattccac ggcggagacc 5940 tatggcaaga tcgtccacta caaggtgagc acggccgcag ggagacctgg cctctcccgg 6000 taggcgctcc caggctatcg cctcctctcc ctctgagcag gagcacctct ctctgccgct 6060 ggtggacgaa ggggtccctg aggaggctcg agacttcatt cagcggttgc tgtgtccccc 6120 ggagacacgg ctgggccggg gtggagcagg cgacttccgg acacatccct tcttctttgg 6180 cctcgactgg gatggtctcc gggacagcgt gccccccttt acaccggatt tcgaaggtgc 6240 caccgacaca tgcaacttcg acttggtgga ggacgggctc actgccatgg tgagcggggg 6300 cggggtaggt acctgtggcc cctgctcggc tgcgggaacc tccccatgct ccctccataa 6360 agttggagta aggacagtgc ctaccttctg gggtcctgaa tcactcattc cccagagcac 6420 ctgctctgtg cccatctact actgaggacc cagcagtgac ctagacttac agtccagtgg 6480 gggaacacag agcagtcttc agacagtaag gccccagagt gatcagggct gagacaatgg 6540 agtgcagggg gtgggggact cctgactcag caaggaaggt cctggagggc tttctggagt 6600 ggggagctat ctgagctgag acttggaggg atgagaagca ggagaggact cctcctccct 6660 taggccgtct ctcttcaccg tgtaacaagc tgtcatggca tgcttgctcg gctctgggtg 6720 cccttttgct gaacaatact ggggatccag cacggaccag atgagctctg gtccctgccc 6780 tcatccagtt gcagtctaga gaattagaga attatggaga gtgtggcagg tgccctgaag 6840 ggaagcaaca ggatacaaga aaaaatgatg gggccaggca cggtggctca cgcctgtaac 6900 cccagcaatt tggcaggccg aagtgggtgg attgcttgag cccaggagtt cgagaccagc 6960 ctgggcaatg tggtgagacc cccgtctcta caaaaatgtt ttaaaaattg gttgggcgtg 7020 gtggcgcatg cctgtatact cagctactag ggtggccgac gtgggcttga gcccaggagg 7080 tcaaggctgc agtgagctgt gattgtgcca ctgcactcca gcctgggcaa cggagagaga 7140 ctctgtctca aaaataagat aaactgaaat taaaaaatag gctgggctgg ccgggcgtgg 7200 tggctcacgc ctgtaatctc agcactttgg gaggccgagg cgggtggatc acgaggtcag 7260 gagatcgaga ccatcttggc taacacggtg aaaccccatc tctcctaaaa atacaaaaaa 7320 ttagccaggc gtggtggcgg gcgcctgtag tcccagctac tcaggaggct gaggcaggag 7380 aatggcgtga acccgggagg cagagtttgc agtgagccga gatcgtgcca ctgcactcca 7440 gcctgggcga cagagcgaga ctctgtctca gaaaaaaaaa aaaaaaaaaa aaaaaatagg 7500 ctggaccgcg gccgggcgct gtggctcatg cctgtaatcc cagcactttg ggagtccaag 7560 gccggtgggt catgagatca ggagttttga gactaggctg gccaacacgg tgaaaccccg 7620 tctctactaa aaatacaaga aaattagctg ggtgtggtct cgggtgcctg taattccagt 7680 tactggggaa gctgaggcag gagaattgct tgaacctggg aggcagagtt tgcagtgagc 7740 caagatcatg ccactacact ccagtctggg tgacagagtg agactctgtc tcaaaaaaaa 7800 aaaaaaaaaa aagggttggg caaggtggtt cacgcctgta atcccagaac tttgggaggc 7860 tgaggcaggc agatcactgg aagtcaggag ttcaagacca gcctggccaa catggtgaaa 7920 ccctgtgtct actaaaaata caaaatttag ccaggcttgg tggcgtatgc ctgtaatgcc 7980 agctactcag gaggctgagg caggagaatc gcttgattga acctgggagg cagagtttgc 8040 agtgggctgg ggttgtgcca ctgcactcta ggctgggaga cagcaagact ccatctaaaa 8100 aaaaaaaaca gaactgggct gggcacagtg gcttatattt gtaatcccag cactttggga 8160 ggctgaggtt ggaggactgc ttgagcccag agtttgggac tacaacagct gaggtaggcg 8220 gatcacttga ggtcagaaga tggagaccag cctggccagc gtggcgaaac cccgtctcta 8280 ccaaaaatat aaaaaattag ccaggcgtgg tagagggcgc ctgtaatctc agctactcag 8340 gacgctgagg caggagaatc gcctgaacct gggaggcgga ggttgcagtg agctgagatt 8400 gcaccactgc actccagcct gggtaacaga gcgagactcc gtatcaaaga aaaagaaaaa 8460 agaaaaaatg ctggaggggc cactttagat aagccctgag ttggggctgg tttgggggga 8520 acatgtaagc caagatcaaa aagcagtgag gggcccgccc tgacgactgc tgctcacatc 8580 tgtgtgtctt gcgcaggaga cactgtcgga cattcgggaa ggtgcgccgc taggggtcca 8640 cctgcctttt gtgggctact cctactcctg catggccctc aggtaagcac tgccctggac 8700 ggcctccagg ggccacgagg ctgcttgagc ttcctgggtc ctgctccttg gcagccaatg 8760 gagttgcagg atcagtcttg gaaccttact gttttgggcc caaagactcc taagaggcca 8820 gagttggagg accttaaatt ttcagatcta tgtacttcaa aatgttagat tgaattttaa 8880 aacctcagag tcacagactg ggcttcccag aatcttgtaa ccattaactt ttacgtctgt 8940 agtacacaga gccacaggac ttcagaactt ggaaaatatg aagtttagac ttttacaatc 9000 agttgtaaaa gaatgcaaat tctttgaatc agccatataa caataaggcc atttaaaagt 9060 attaatttag gcgggccgcg gtggctcacg cctgtaatcc tagcactttg ggaggccaag 9120 gcaggtggat catgaggtca ggagatcgag accatcctgg ctaacacggt gaaaccccgt 9180 ctctactaaa aatacaaaaa aattagccgg gcatggtggc gggcgcttgc ggtcccagct 9240 acttgggagg cgaggcagga gaatggcatg aacccgggag gcggagcttg cagtgagccg 9300 agatcatgcc actgcactcc agcctgggcg acagagcaag actccgtctc aaaaaaaaaa 9360 aaaaaaaagt atttatttag gccgggtgtg gtggctcacg cctgtaattc cagtgctttg 9420 ggaggatgag gtgggtggat cacctgaggt caggagttcg agaccagcct gaccaacgtg 9480 gagaaacctc atctctacta aaaaacaaaa ttagccaggc gtggtggcat atacctgtaa 9540 tcccagctac tcaggaggct gaggcaggag aatcagaacc caggaggggg aggttgtggt 9600 gagctgagat cgtgccattg cattccagcc tgggcaacaa gagtgaaact tcatctcaaa 9660 aaaaaaaaaa aaaaagtact aatttacagg ctgggcatgg tggctcacgc ttggaatccc 9720 agcactttgg gaggctgaag tggacggatt gcttcagccc aggagttcaa gaccagcctg 9780 agcaacataa tgagaccctg tctctacaaa aaattgaaaa aatcgtgcca ggcatggtgg 9840 tctgtgcctg cagtcctagc tactcaggag tctgaagtag gagaatcact tgagcctgga 9900 gtttgaggct tcagtgagcc atgatagatt ccagcctagg caacaaagtg agacctggtc 9960 tcaacaaaag tattaattac acaaataatg cattgcttat cacaagtaaa ttagaaaata 10020 cagataagga aaaggaagtt gatatctcgt gagctcacca gatggcagtg gtccctggct 10080 cacacgtgta ctgacacatg tttaaatagt ggagaacagg tgtttttttg gtttgttttt 10140 ttccccttcc tcatgctact ttgtctaaga gaacagttgg ttttctagtc agcttttatt 10200 actggacaac attacacata ctatacctta tcattaatga actccagctt gattctgaac 10260 cgctgcgggg cctgaacggt gggtcaggat tgaacccatc ctctattaga acccaggcgc 10320 atgtccagga tagctaggtc ctgagccgtg ttcccacagg agggactgct gggttggagg 10380 ggacagccac ttcatacccc agggaggagc tgtccccttc ccacagctga gtggggtgtg 10440 ctgacctcaa gttgccatct tggggtccca tgcccagtct taggaccaca tctgtggagg 10500 tggccagagc caagcagtct ccccatcagg tcggcctccc tgtcctgagg ccctgagaag 10560 aggggtctgc agcggtcaca tgtcaaggga ggagatgagc tgaccctaga acatgggggt 10620 ctggacccca agtccctgca gaaggtttag aaagagcagc tcccaggggc ccaaggccag 10680 gagaggggca gggcttttcc taagcagagg aggggctatt ggcctacctg ggactctgtt 10740 ctcttcgctc tgctgctccc cttcctcaaa tcaggaggtc ttggaagcag ctgcccctac 10800 ccacaggcca gaagttctgg ttctccacca gagaatcagc attctgtctc cctccccact 10860 ccctcctcct ctccccaggg acagtgaggt cccaggcccc acacccatgg aactggaggc 10920 cgagcagctg cttgagccac acgtgcaagc gcccagcctg gagccctcgg tgtccccaca 10980 ggatgaaaca gtaagttggt ggaggggagg gggtccgtca gggacaattg ggagagaaaa 11040 ggtgagggct tcccgggtgg cgtgcactgt agagccctct agggacttcc tgaacagaag 11100 cagacagaaa ccacggagag acgaggttac ttcagacatg ggacggtctc tgtagttaca 11160 gtggggcatt aagtaagggt gtgtgtgttg ctggggatct gagaagtcga tctttgagct 11220 gagcgctggt gaaggagaaa caagccatgg aaggaaaggt gccaagtggt caggcgagag 11280 cctccagggc aaaggccttg ggcaggtggg aatcctgatt tgttcctgaa aggtagtttg 11340 gctgaatcat tcctgagaag gctggagagg ccagcaggaa acaaaaccca gcaaggcctt 11400 ttgtcgtgag ggcattaggg agctggaggg attttgagca gcagagggac ataggttgtg 11460 ttagtgtttg agcaccagcc ctctggtccc tgtgtagatt tagaggacca gactcaggga 11520

tggggctgag ggaggtaggg aagggagggg gcttggatca ttgcaggagc tatggggatt 11580 ccagaaatgt tgaggggacg gaggagtagg ggataaacaa ggattcctag cctggaacca 11640 gtgcccaagt cctgagtctt ccaggagcca caggcagcct taagcctggt ccccatacac 11700 aggctgaagt ggcagttcca gcggctgtcc ctgcggcaga ggctgaggcc gaggtgacgc 11760 tgcgggagct ccaggaagcc ctggaggagg aggtgctcac ccggcagagc ctgagccggg 11820 agatggaggc catccgcacg gacaaccaga acttcgccag gtcgggatcg gggccggggc 11880 cggggccggg atgcgggccg gtggcaaccc ttggcatccc ctctcgtccg gcccggacgg 11940 actcaccgtc cttacctccc cacagtcaac tacgcgaggc agaggctcgg aaccgggacc 12000 tagaggcaca cgtccggcag ttgcaggagc ggatggagtt gctgcaggca gagggagcca 12060 caggtgagtc cctcatgtgt ccccttcccc ggaggaccgg gaggaggtgg gccgtctgct 12120 ccgcggggcg tgtatagaca cctggaggag ggaagggacc cacgctgggg cacgccgcgc 12180 caccgccctc cttcgcccct ccacgcgccc tatgcctctt tcttctcctt ccagctgtca 12240 cgggggtccc cagtccccgg gccacggatc caccttccca tgtaagaccc ctctctttcc 12300 cctgcctcag acctgctgcc cattctgcag atcccctccc tggctcctgg tctccccgtc 12360 cagatatagg gctcacccta cgtctttgcg actttagagg gcagaagccc tttattcagc 12420 cccagatctc cctccgttca ggcctcacca gattccctcc gggatctccc tagataacct 12480 ccccaacctc gattcccctc gctgtctctc gccccaccgc tgagggctgg gctgggctcc 12540 gatcgggtca cctgtccctt ctctctccag ctagatggcc ccccggccgt ggctgtgggc 12600 cagtgcccgc tggtggggcc aggccccatg caccgccgcc acctgctgct ccctgccagg 12660 gtacgtccgg ctgcccacgc ccccctccgc cgtcgcgccc cgcgctccac ccgccccttg 12720 ccacccgctt agctgcgcat ttgcggggct gggcccacgg caggagggcg gatcttcggg 12780 cagccaatca acacaggccg ctaggaagca gccaatgacg agttcggacg ggattcgagg 12840 cgtgcgagtg gactaacaac agctgtaggc tgttggggcg ggggcggggc gcagggaaga 12900 gtgcgggccc acctatgggc gtaggcgggg cgagtcccag gagccaatca gaggcccatg 12960 ccgggtgttg acctcgccct ctccccgcag gtccctaggc ctggcctatc ggaggcgctt 13020 tccctgctcc tgttcgccgt tgttctgtct cgtgccgccg ccctgggctg cattgggttg 13080 gtggcccacg ccggccaact caccgcagtc tggcgccgcc caggagccgc ccgcgctccc 13140 tgaaccctag aactgtcttc gactccgggg ccccgttgga agactgagtg cccggggcac 13200 ggcacagaag ccgcgcccac cgcctgccag ttcacaaccg ctccgagcgt gggtctccgc 13260 ccagctccag tcctgtgatc cgggcccgcc ccctagcggc cggggaggga ggggccgggt 13320 ccgcggccgg cgaacggggc tcgaagggtc cttgtagccg ggaatgctgc tgctgctgct 13380 gctgctgctg ctgctgctgc tgctgctgct gctgctgctg ctgctggggg gatcacagac 13440 catttctttc tttcggccag gctgaggccc tgacgtggat gggcaaactg caggcctggg 13500 aaggcagcaa gccgggccgt ccgtgttcca tcctccacgc acccccacct atcgttggtt 13560 cgcaaagtgc aaagctttct tgtgcatgac gccctgctct ggggagcgtc tggcgcgatc 13620 tctgcctgct tactcgggaa atttgctttt gccaaacccg ctttttcggg gatcccgcgc 13680 ccccctcctc acttgcgctg ctctcggagc cccagccggc tccgcccgct tcggcggttt 13740 ggatatttat tgacctcgtc ctccgactcg ctgacaggct acaggacccc caacaacccc 13800 aatccacgtt ttggatgcac tgagaccccg acattcctcg gtatttattg tctgtcccca 13860 cctaggaccc ccacccccga ccctcgcgaa taaaaggccc tccatctgcc caaagctctg 13920 gactccacag tgtccgcggt ttgcgttgtg ggccggaggc tccgcagcgg gccaatccgg 13980 aggcgtgtgg aggcggccga aggtctggga ggagctagcg ggatgcgaag cggccgaatc 14040 agggttgggg gaggaaaagc cacggggcgg ggctttggcg tccggccaat aggagggcga 14100 gcgggccacc cggaggcacc gcccccgccc agctgtggcc cagctgtgcc accgagcgtc 14160 gagaagaggg ggctgggctg gcagcgcgcg cggccatcct ccttccactg cgcctgcgca 14220 cgccacgcgc atccgctcct gggacgcaag ctcgagaaaa gttgctgcaa actttctagc 14280 ccgttccccg cccctcctcc cggccagacc cgccccccct gcggagccgg gaattccgag 14340 gggcggagcg caggccgaga tggggaatgt gggggcctgc agaggaccct ggagacggag 14400 gcgtgcagaa g 14411 <210> SEQ ID NO 3 <211> LENGTH: 15000 <212> TYPE: DNA <213> ORGANISM: mus musculus <400> SEQUENCE: 3 cagtgtcccc actgcccaag gctggctcca tcacgtaccg ctttggctca gctggccagg 60 acacacagtt ctgcctgtgg gacctcacag aagatgtgct ctcccctcat ccgtctctgg 120 cccgtacccg cacccttccg ggcacacctg gtgccacccc accagcttct ggtagttctc 180 gggccggaga gacaggtgca ggccccctgc cccgctccct gtctcgttcc aacagtctcc 240 cacacccagc tggtggtggc aaggctggtg ggcctagtgc atcgatggag cctggcatac 300 cattcagcat tggccgcttt gccacactga ccctgcagga gcggcgggac cggggagctg 360 agaaggaaca caaacgctac catagcctgg gaaacatcag ccgcggtggc agtgggggca 420 atagcagcaa tgacaagctc agtggtcctg ccccccgaag ccgattggac ccagctaagg 480 tgctgggcac ggcactgtgc cctcggatcc atgaggtgcc actgctggag cctctcgtgt 540 gcaagaagat tgctcaggaa cgcctgaccg tgctactgtt cctggaggat tgtatcatca 600 ctgcctgcca agagggcctc atctgcacct gggcccggcc aggcaaggcg gtgagtccgc 660 acctgcccaa gcgctgaggg gcaccagttc tgtccctacc ggatgccagt tatccgtcag 720 cagaaaggtc aggtatagga gacagaatgg ggggaaccac agctaacgtc tttagagcct 780 ctgctggccc atatggctca tccttagtac ttcacactca aggcagaacc tgtgtttata 840 ggaaatctga agtgtagatg gtgaaacttt attcaggtct agggatgtga ttgagctggg 900 ggcccacttc tggcctgcct cttagacact gtttctgagc cagctgctga aggcctggat 960 gggaattagc cagggtccag gcctgcactt cctcttgctg ctgtgtggtc ctggtcattg 1020 ggtctcacag atgggctgtg cagtggctgt gctcttagtt ggtgaggtgc aggcctgtca 1080 cctggtcagg cttgagcatg tggtctcagt gtctaggacc ctactctgcc ctcagtcctt 1140 cagtcccttg ctttggaagg ctagagtcca gaagccttag aacgtcaggc agttgcagag 1200 ccactgccag gctagtaggg ctgcgggagt tgactgagtt ctcacagaca cccctctgtc 1260 tccctagttc acagacgagg agaccgaggc ccaggcaggg caagcaagtt ggcccaggtc 1320 acccagcaag tcagttgtag aggtaggaca acccctgaag ctgcaagtgg accccagttt 1380 cttttctctc cactgtcgtc ccctgtatgc ccaggacacc tggggccaca ttactgtgga 1440 agtgctactc tgggtcagtg gagacggccg agctgtttgt tcctagctag gacagcagct 1500 ttaggcctgg ggggcagatc ccagctgggg cagcagctcc aaggcctttg ggtggctcct 1560 tctccgggtt ctggcagaag cccaggtgct gtctaatcca cctttctcct cttgttctcc 1620 ccagggcatc tcctcccaac caggcagctc ccccagtggc actgtggtgt gaaatgtgga 1680 tgtcccatgt tcccggcctc ctagccataa ccctccccgc tgacctcaag aatcactgta 1740 ttaacaagac taatcatgat ggaaggactg ctccaagccc cacgctgcac acatactggg 1800 ggtcccctag gttggcccag ccatggggat gtagtgtcct gtgtggcctt ggccctgtcc 1860 tccacccact gccaagtaca atgacctgtt ctctgaaaca tcagtgttaa ccatatccct 1920 gtcccagcat gtgactgttc actcctggga gagacttagc ccacagtacc cctgggtgag 1980 agggcagggc aggggccatc cccactcctg cccaaactcc accccttgct atggtctgtg 2040 attttgaaag tgttaaatta tggaagccct gagggccctc cttgttcccc tggacctctt 2100 atttatacta aagtccttgt ttgcacagtg tttctgttcc ctggggcagg gtagggtggg 2160 ggttgcagta cttggcctcc aagctgtgct ctgaccaaag gaagcccaat cttagctgtt 2220 tccccatccc tagccccgag cagagagccc tctgaaagat gagtctcgac ccccaaagtc 2280 aagaggctga gatggccttc ctactaggtc cttggagatg tttgaaactt gttttaaaca 2340 ccaggactat ccaagcatgc tctccttggg gagaggagga tgctggaatt gactgcactc 2400 cctgcctcct ctgaacatgc ctttgcagtc tgctgcccct ggcccattta tgactggcca 2460 tctagtgcca gctggaggtc atgatttcct ccccagagaa ctggccaccc tagaaagaag 2520 ctaacttgtc gcctggcttg ctgtccaggc agctccgccc tcaaccccta aaatgtttct 2580 gtctctaatc ctagcccagg caggaatgtg gctgccccgg cctgtggcca aggagctatt 2640 ttggggttct cttttgctta aggagggcct ggatccacca cttgcctccc ccaggctggg 2700 gccagcaggt cacccctggc cctggcggct gagcaaactc tctcctgatc ttccttctac 2760 ctcctgccaa aaaatggggg ggcgggtaat acagcaggca caggggctaa atttaactgt 2820 cccaaagtcg gaatccattg ctgagtcacg aagaagctgc ccctggcctt tgcccccccc 2880 actaccccct caccccctgt tgcccaggca tcagcccttt cccccaaccc ctcccagctc 2940 tgagtctata gactggctct cctgggcact gacacctccc acctgtaact ccctgtgctc 3000 tctttatggg tgggtagagt caatgggggg gggcaaccct ggagtattac tctgtcccct 3060 gacattgggc tctgaagagt tttgaggggc cctggaagaa gggagttggg gtgttggctc 3120 aggaggggtt aaaaactggg aggcgggagg ggggctgggc caaggggtgg agaaaagagg 3180 aggaggcctt aagcatagaa ctggccagag agacccaagg gatagtcagg gacgggcaga 3240 catgcagcta gggttctggg gcctggacag gggcagccag gccctgtgac gggaagaccc 3300 cgagctccgg cccggggagg ggccatggtg ttgcctgccc aacatgtcag ccgaagtgcg 3360 gctgaggcag ctccagcagc tggtgctgga cccaggcttc ctgggactgg agcccctgct 3420 cgaccttctc ctgggcgtcc accaggagct gggtgcctct cacctagccc aggacaagta 3480 tgtggccgac ttcttgcagt ggggtgagta tggataggaa gcctggggtt gggtgcaagg 3540 cagaggtggg tctacagggc aagaatgggc tatggagggg caggagggcc tggaaagggc 3600 tttttgtaag ggagccaagc agagctcatg acctgacccc aagctcccct ggtgaggcac 3660 cagggtcagt gaggccacct atgactcagc cagtgcaggc tggggtgggc atagcctcct 3720 gctatctcag cacccacact aggacctggc agctttctct tttaggaccc ttggctcctc 3780 aaactggctt catagccctc cccagtttcc cagagtgtgg ggagggacag cgtggggcag 3840 ctgccagggt gtggcccata ggcaggtgtt tggcgtctgc ctccccagct gccctgacag 3900 gtgtccagga gctatgaggg cactgtgact cacagaggcc ctgggggaga accagcccgg 3960 cagacaggcg ccaccgagca ccctttctgt tccccaaatt aagaggaagg aacaacttca 4020 gcttctgagt gtgcccatcc ctagcactct gatcccgccc agcctttgtg ggccagattg 4080 gtcatccctc ctggcttctc atctgctttt gtggttctag ctcaagacct ctaattcctc 4140 tgctgactta aatgcccttc cccagaggtc ttctcaggcc tagtggacaa gcttggagcc 4200 ttatctgctc ctgcccaaca ttgagccaaa gctccagctt accccagctt ccttacaagt 4260 aacgacctgt tttgttgctc tgtgcctatt attaagggtc caggtcttga ttcttggctg 4320 tctgcccatg tgtgtgaccc tagtgcattc tcccctcctc ccccgtttca cagatggaaa 4380

ggttgaggcc atcggttaga ctgctaagcc tgtgaaagac tttttctcct ctccagtctt 4440 tagtgtctcc ctcaaccttt cttttgaagg atggggtttg ggctggcagg gtaaactgag 4500 aactggggtg ggggcagggg gtctgaccct ctgggaagga gcagtccttt tgtggcctga 4560 gcagcatcct gtgggcccct cccctgccag gcctgggcgg gggagggggc ctgggttccc 4620 gctgccttaa aagggctcaa cgccttggct ctctcctcct ccccaccccc cagccttggc 4680 cctagctgta tcttccccgg ctgcccactt tcccaaaccc ctttcttctc tgtgacccca 4740 tctccccgct tccccacacg tccctcctcc atccttactc cccggcctta gaacttccct 4800 aagggagatc tgacctccct ctgcccaccc cgcaccccca gtcgccagcc tcagacctag 4860 ctgctctccc ctctggctga accaccctag cacaggacct tataccctgg agctttggtt 4920 ataagaagac tctccttcac cctttggaaa ccaagaaagc ccttccaaca gtgtccagga 4980 tgctggaggg cagtgaccct cccccacttc ttcttcgtgc tggctgtgct gacacagctc 5040 cagttcgagg ttgtggcccg agacattaag tgagagcccc gggtgacctg acttagcacc 5100 ctgatcatca catgggagtg aaaggcctga tgcgccagct tctcccactg cctccctttc 5160 tgccctgcaa ccctgtggaa acaggcagtt ctgggtccca caaacatcac agaggttttg 5220 aaagcagaat cctaaagccg atttaagggg cagaaggaag gaggctataa agtcactacc 5280 cttaccgcta gtgttctgat gacccttggt tcttcttccc ccacccccgc ccagtggagc 5340 ccattgcagc aaggcttaag gaggtccgac tgcagaggga tgattttgag attttgaagg 5400 tgatcgggcg tggggcgttc agcgaggtga gtcttcagtg gcctgggaat ggaactttac 5460 ttgatgtggg tggggcataa cagctggggc agagccttaa aaattgatga atgagcttga 5520 atttaaggct ggaggggtgg gggcggagct tgtggtcagt gggcggtgtg cacgtgaggg 5580 cggggctaag gttgggtgga gataagggtg gagtcctgtc tgggtgagcc ttgctggttt 5640 tccctgccac ctcttgctgt catctcggtt ccgtatttag gtagcggtgg tgaagatgaa 5700 acagacgggc caagtgtatg ccatgaagat tatgaataag tgggacatgc tgaagagagg 5760 cgaggtgagg gccagggatt agggcagcgc cctcatctct ccaactcacc tcctgtagct 5820 tctctcctac ctcacaggtg tcgtgcttcc gggaagaaag ggatgtatta gtgaaagggg 5880 accggcgctg gatcacacag ctgcactttg ccttccagga tgagaactac ctggtaagct 5940 ccgggttcag gtgactagga aagagtgaca gttacatcgc cccaagtcaa gaaggctgga 6000 gaagggagaa gctgctgtag atcggggggg tgggggtggg ggggacacac acaggggatg 6060 ggggacgggg gtaggattgt gtctcaagta taggagagac cttccttgag acaggagtga 6120 tatctggttt ggcctttgga tggggcgctc tctcactgtg cgggggtcct ctgtgcttgg 6180 gaacggggtg tctttgggag tcttgggggc taccaaaccc ctgtgacaca cccgctccca 6240 gtacctggtc atggaatact acgtgggcgg ggacctgcta acgctgctga gcaagtttgg 6300 ggagcggatc cccgccgaga tggctcgctt ctacctggcc gagattgtca tggccataga 6360 ctccgtgcac cggctgggct acgtgcacag gtgggcgtgg cggggccctt ggagggttag 6420 cagaatttgt gtgggaagga agggtacctg aaggtcagat cccattgggg acagaatcgg 6480 ggtctagaat tgtagaatcc tgggtggggt ggaagtggat cgagctgacg ggccctaaga 6540 gggaaggttt tcaagaaagc acactttccc tcttctctct atgcacaggg acatcaaacc 6600 agataacatt ctgctggacc gatgtgggca cattcgcctg gcagacttcg gctcctgcct 6660 caaactgcag cctgatggaa tggtaagaag agcctggcga aactctcctc attggtgaag 6720 gaccggatta gggggcgggg ctgggttgag gagcaggagg ggagcttggt ctgggatgtc 6780 ctgcgcacca tatttggaca gtcaagggaa aggttttaag cattcaggtc tgattggcac 6840 aggtgaggtc gctggtggct gtgggcaccc cggactacct gtctcctgag attctgcagg 6900 ccgttggtgg agggcctggg gcaggcagct acgggccaga gtgtgactgg tgggcactgg 6960 gcgtgttcgc ctatgagatg ttctatgggc agaccccctt ctacgcggac tccacagccg 7020 agacatatgc caagattgtg cactacaggg tgagcacaag caccatgcag gggggctgac 7080 ttagtggctt gtgctcccag actgtctttt ttaaaagata tttatttata tgtgtgtgtt 7140 ttctgtgtat gtatatctgt gcactgagta ggtgtgcgaa ggtcagaggg catgggatcc 7200 cctggaactg gagtcacaga ctattgtgtg ctgccatgct gagtgctggg aaacagaacc 7260 ttgatcactc tgcaagagca gccagtgcac tgaaacgaca gagccagctc tgcagcccag 7320 ggctaactgt tgcttttctt tctaaatagg aacacttgtc gctgccgctg gcagacacag 7380 ttgtccccga ggaagctcag gacctcattc gtgggctgct gtgtcctgct gagataaggc 7440 taggtcgagg tggggcaggt gatttccaga aacatccttt cttctttggc cttgattggg 7500 agggtctccg agacagtgta ccccccttta caccagactt cgagggtgcc acggacacat 7560 gcaatttcga tgtggtggag gaccggctca ctgccatggt gagcgggggc ggggtacgta 7620 cctgcagttc ctgatccgtt gaggggactt ccctagcctc ttccataaaa ttggggtgat 7680 tggccaggtg tggtggtgca tacctttaat cgtagaactt cataggcaga ggcaggtggc 7740 tctctggtaa atcaaggcca tcttggtcta catagtgact tctaggccag tcaggagtga 7800 gatcctccct tgaaaaataa aaaagggggt gttgaccttc ctgggtccca aattattatc 7860 ctagagcact gctatgtatc cactcaggta tgaggacaca caggtgacca gtcccaaaga 7920 cagtgagtga ggcctcactc ttggcagtac taaaattgat tgtagggggc tgggctcttg 7980 acccagcctg gaaagtgctg gagggcttcc tggaggagga gactagctga gcccagaagg 8040 atgcaggaga tcctttctcg ggtgagtgct ctcagcattt taacaagctc taggccctgc 8100 agagagaagt ctggtgtggg cagagcccca atagaaagca acaagataga agagaaaatg 8160 gtggagtttg ttagtggggg cagttatgcc gtgaacatag aggggcgaag ggccatctcg 8220 gataactgct agccacaaga gccctgtctg tcttcctagg agacgctgtc agacatgcag 8280 gaagacatgc cccttggggt gcgcctgccc ttcgtgggct actcctactg ctgcatggcc 8340 ttcaggtgag cacgactgcc ccctgctggg gcctgtgtgc aggcccacca cagccactca 8400 attgaaggct cagtcttcaa accaagtatt cctaggagct gtctaagtta ggctttctgc 8460 tgctgcgatg aaccctgact aaaagcaagc tggggaggaa aaggcttatc gggcttacgt 8520 ttccacatgg gagcccatca ctgaaggaag ccaggacagg aactcacagc ggggcaggaa 8580 cgtggagctg atgcagaggc aatggagggg agctgcttac tgacttgatc cttatgtctt 8640 cctcagcctg tttccttgta gagcccagga ccaccaggcc agtgagggct ccactcacaa 8700 tgggctgagc tctcatctat gatcactagt tatgaaaatg cccgataggc ttgcctgcag 8760 cttcagtttt tgaggcactt tccttccttc cttccttcct tccttccttc cttccttcct 8820 ttctttcttt ctttctttct ttctttcttt ctttctttct ttctttcttt ctttctttct 8880 tagtctttta gagacagggt ctttctatgt agctctggct gtcttggaat tcattctgta 8940 gaccaggctg gtcttattta tttattttat gtatgtgagt ccactatcac tgtcctcaga 9000 cacaccagaa gagggcatca gatcccatta cagatggctg tgagccacca tgtggttgct 9060 gggaattgaa ctcaggacct ctggaagagc agccagtgct cctgccctgt agaggcattt 9120 tcttcatgaa ggctgtctcc tctctgatga cttgatgact ctagcttgtt gtgtcaagtg 9180 gacataagac taggaaagca gctacacatg cactttgttt atttttgttt tgctttttga 9240 gactgggtct ctccatctca tagctctggc catcctgcct ggtgacattc cagtccagtt 9300 gtataaccta agaatctgag actcagcctt gcagaatcct gctattaacg ggtctaggac 9360 actccataga atccaggatc ttagaaaaac aaacctgaag tgtgacagtt tattttaaga 9420 acacaattgg agcacataac aataatacaa cttttcagtt ttaaaaagtt ttctgtcttg 9480 ttttttgagg caggagctcc ttaatatagt ctaagccgcc ctgcgagtgc tgtgattgat 9540 gggcatgtac caccatgcct agtcaataaa gcctttaaaa agcatccgtt atgctggctg 9600 tggtgccaca aacctgtaat cccagcactt agaaggtaga ggcaagatta tcagaaattc 9660 aaggccatcc tgggctatac agtaatctaa ggctagcctg gtctacaaga gactctgtct 9720 aaagaaacaa aagataaata gcacccacta ttgctaggca atataaccct ataaccccac 9780 cattgaggag gctgaggctg gagcatcact gcaaatttga ggccaggatg gtcaacaaat 9840 aagtcccaga gctggcatag aggaactctg tctcaacaat aaagagaact tatctagcat 9900 ttatgagggt aaataaaaat ttaccattgc cacaaaaaat gtaaatgaag agactgcttt 9960 taggagtgaa ctgggaagca gggaacactt agaggatgct cactcacaca ggtatccacc 10020 atcaggcatg cctcaggcct gcacagggaa ggacaacttg tttcatgatt tgcaagcagc 10080 atcccatgct ccttagagcg ggttgggccc agcccaccct ctgtggagtt atcgctcagc 10140 caggcagcaa ggcagccaag gtgctgaggc cctggcagtc tgctctcttc tctgctctga 10200 acctccttta gctttagcct aggagcctgg cctggtgccc acaggctagg gagtccctag 10260 cctcttcctc ttctcagaga caatcaggtc ccggacccca cccctatgga actagaggcc 10320 ctgcagttgc ctgtgtcaga cttgcaaggg cttgacttgc agcccccagt gtccccaccg 10380 gatcaagtgg tgagtagact gagaggtggg caaagcttcc tgggtgggtg tacctgcagt 10440 gccaactgcc aggctgttaa ttcagtagga cactgtcccc aactggccca actgcacatc 10500 ctgtagtcag gaggcacagg cagaaaaatc ccaaattcaa ggcttgctcc cgttatgtaa 10560 tgagatcctg tcttggagta aaaaacaaag aagagaacta gggatagctc agaggtagat 10620 gctctcctgg catgggggtg gggtcagaaa gcaacaccaa ccggggcctg ggagggaggg 10680 actgccaacc acctggagga gtctggggta gacttggtga acaaagttca gaggccatca 10740 ggtgggatgc tggtttctta aaagccacag ataggtgggt agcattggaa agaggagtgg 10800 ggggttgcag aaagtgacaa gacacaaact ggggaggcct aagggtaaag ccagggttgt 10860 ctgaagcact gtggagctgg gaggaacacg ctaaacttct gacttcagcc cttcagttcc 10920 cctgttgact acactgtccc cagggaccca gggatgggga gaggtggacg ggggagggaa 10980 gtacgggact gatccagctc caggtcccaa ctctgatccc caccgacagg ctgaagaggc 11040 tgacctagtg gctgtccctg cccctgtggc tgaggcagag accacggtaa cgctgcagca 11100 gctccaggaa gccctggaag aagaggttct cacccggcag agcctgagcc gcgagctgga 11160 ggccatccgg accgccaacc agaacttctc caggtcaggg tcacagtgct ggggtgaggg 11220 gagaggagag cagcaaccct cgcagtctcc tcaccgatag gtcggctcac tcccctatct 11280 ttcccagcca actacaggag gccgaggtcc gaaaccgaga cctggaggcg catgttcggc 11340 agctacagga acggatggag atgctgcagg ccccaggagc cgcaggcgag tccctcacct 11400 gcttccagcc aagggggcac tgggtggaga tggggggcat gttgggtgtg tgaaccctcg 11460 gggcagggga ggagtccagg ctggggcacc gcagccgcgc cactgccttt ctcctccatc 11520 ctccacactc catacacctc tctcttctcc ttccagccat cacgggggtc cccagtcccc 11580 gggccacgga tccaccttcc catgtaagac ccctctctcc cctccccgat ccccatctta 11640 gatatgctac ccacagccct tctcccgtcc acgtttaggg tccattctcc ttgggggttc 11700 cagaagaaag ctgcccttca ctcatccatt cagcatgcac tatctaccag ctctccctcg 11760 tttcaggctt ctcgccaaat cctccccaag ggaactccct atactcccgt tctggcctcg 11820 actagattcc cgcactgcct ctcgccctgc tgctgggctc cgatcgggtc acctgtccct 11880

tctctctcca gctagatggc cccccggccg tggctgtggg ccagtgcccg ctggtggggc 11940 caggccccat gcaccgccgt cacctgctgc tccctgccag ggtatgtccc acgtccgccc 12000 accacgggcc tctgcctagc tctgcccact gagtgtcacc actgcttgct gtgcctctgt 12060 ggagctcggc ccaccgcagg gagggggggt attcgggcgg ccaatcaaca caggctgctg 12120 ctaagtagcc aatgacgagt tccaacagga gctctttctt gcgagcagac caactttagc 12180 tgcgggctgt ggggaccaga gatgcgctca gaggcccatc tatgggtata ggctgggcgg 12240 ctcccaggag ccagtgggcc cctgtagcct agtgctaatc caaccttctc tcctgcagat 12300 ccctaggcct ggcctatccg aggcgcgttg cctgctcctg ttcgccgctg ctctggctgc 12360 tgccgccaca ctgggctgca ctgggttggt ggcctatacc ggcggtctca ccccagtctg 12420 gtgtttcccg ggagccacct tcgccccctg aaccctaaga ctccaagcca tctttcattt 12480 aggcctccta ggaaggtcga gcgaccaggg agcgacccaa agcgtctctg tgcccatcgc 12540 gccccccccc cccccccacc gctccgctcc acacttctgt gagcctgggt ccccacccag 12600 ctccgctcct gtgatccagg cctgccacct ggcggccggg gagggaggaa cagggctcgt 12660 gcccagcacc cctggttcct gcagagctgg tagccaccgc tgctgcagca gctgggcatt 12720 cgccgacctt gctttactca gccccgacgt ggatgggcaa actgctcagc tcatccgatt 12780 tcactttttc actctcccag ccatcagtta caagccataa gcatgagccc cctatttcca 12840 gggacatccc attcccatag tgatggatca gcaagacctc tgccagcaca cacggagtct 12900 ttggcttcgg acagcctcac tcctgggggt tgctgcaact ccttccccgt gtacacgtct 12960 gcactctaac aacggagcca cagctgcact cccccctccc ccaaagcagt gtgggtattt 13020 attgatcttg ttatctgact cactgacaga ctccgggacc cacgttttag atgcattgag 13080 actcgacatt cctcggtatt tattgtctgt ccccacctac gacctccact cccgaccctt 13140 gcgaataaaa tacttctggt ctgccctaaa tcccgcgcaa tatctctgtt gtggaaagga 13200 aaccgccccg caggccaatg gagagtccaa tagagacaac caatggcttg agtgggagct 13260 agaggggagg caaagcgcac gaatcaggtt gaagggtggg gcttaggcat ccagccagta 13320 ggagagaagc aacaagccac cagagacacc accgcccccc accctccccc ccagctgtga 13380 cccagctgtg ccactcaagt ttggaaaaaa gtagggggtt gggccagcag cgggcacacc 13440 atcttcccac tgcgcctgcg caagccacgc gcatccgctt tttggaccga cactccagaa 13500 aagttgctgc aaactttcta gcgcgattcc ccgcccctcc tcccagctag atccaccgcc 13560 tacccgcggg gccgggaatt ccgaggggcg gagcacggcg cggagatggg aagggagggg 13620 gcccttcaag ggacccggga gatgggagcg gcttcgcgcc cttaaccctc cggacggccc 13680 attaccttct ccgttgctct gatagggaaa ctgaggccct gagtcagagg cacacaaggg 13740 gggaaggcca aaagcgcggc cagagacgga gggaaaacaa agaatcctga cagcccggga 13800 ggggggcgga cacacaggga caaggacaga cccgagtgca gagctgggtc tagtctttgg 13860 gagggggcca gaagactgca aggggaccgg gggggggggc ggcgaggagg actgggcgga 13920 ggagggggct ggggaagccc gcgggaggcg gcaaaggagg gaggaacttt ccaaagttgc 13980 caaacatggc tacctcgcct gcggagccga gcgcggggcc cgcggctcgg ggggaggcgg 14040 cggcggcgac cgaggagcag gaggaggaag cgcgccagct tctgcagact ctgcaggcag 14100 ccgaggggga ggcggcggcg gccggggcgg gagatgcggc ggcggcggcg gactctgggt 14160 ccccgagtgg cccggggtct ccccgggaga ccgtgaccga ggtgcccact ggccttcgct 14220 tctcgcccga acaggtggca tgcgtgtgcg aggcgctgct gcaggcgggc cacgccggcc 14280 gcttgagccg cttcctgggc gcgctgcccc cggccgagcg cctacgtggc agcgatccgg 14340 tgctgcgcgc gcgggcccta gtggccttcc agcggggtga atacgccgag ctctaccaac 14400 ttctcgagag ccgccctttc cccgccgccc accacgcctt cctgcaggac ctctacctgc 14460 gcgcgcgcta ccacgaggcc gagcgggccc gtggccgtgc gctgggcgct gtggacaaat 14520 accggctgcg caagaagttc cctctgccca agaccatctg ggatggcgag gagaccgtct 14580 attgcttcaa ggagcgctcg cgagcggcgc tcaaggcctg ctaccgcggc aaccgctatc 14640 ccacgcctga cgagaagcgc cgcctggcca cgctcaccgg cctctcgctt acacaggtca 14700 gcaactggtt caagaaccgg cgacagcgcg accgcactgg gaccggcggt ggagcgcctt 14760 gcaaaaggtg aggggggaac cgaccctcct tcctcggtgg ccgctggagt ctgcgcaagt 14820 gacccttcac atccctcttc ggtggcgtcg gcgagtgcat aggctgagcg tggagagacc 14880 aggcacaccc tgggttctct gggcatcact gcctcagggg cagaggttgt tccagctact 14940 tctaagctgg gaacgcagtg ccaggaatgg gggggggggc gggggcggga cgggcagtga 15000 <210> SEQ ID NO 4 <211> LENGTH: 1896 <212> TYPE: DNA <213> ORGANISM: mus musculus <400> SEQUENCE: 4 atgtcagccg aagtgcggct gaggcagctc cagcagctgg tgctggaccc aggcttcctg 60 ggactggagc ccctgctcga ccttctcctg ggcgtccacc aggagctggg tgcctctcac 120 ctagcccagg acaagtatgt ggccgacttc ttgcagtggg tggagcccat tgcagcaagg 180 cttaaggagg tccgactgca gagggatgat tttgagattt tgaaggtgat cgggcgtggg 240 gcgttcagcg aggtagcggt ggtgaagatg aaacagacgg gccaagtgta tgccatgaag 300 attatgaata agtgggacat gctgaagaga ggcgaggtgt cgtgcttccg ggaagaaagg 360 gatgtattag tgaaagggga ccggcgctgg atcacacagc tgcactttgc cttccaggat 420 gagaactacc tgtacctggt catggaatac tacgtgggcg gggacctgct aacgctgctg 480 agcaagtttg gggagcggat ccccgccgag atggctcgct tctacctggc cgagattgtc 540 atggccatag actccgtgca ccggctgggc tacgtgcaca gggacatcaa accagataac 600 attctgctgg accgatgtgg gcacattcgc ctggcagact tcggctcctg cctcaaactg 660 cagcctgatg gaatggtgag gtcgctggtg gctgtgggca ccccggacta cctgtctcct 720 gagattctgc aggccgttgg tggagggcct ggggcaggca gctacgggcc agagtgtgac 780 tggtgggcac tgggcgtgtt cgcctatgag atgttctatg ggcagacccc cttctacgcg 840 gactccacag ccgagacata tgccaagatt gtgcactaca gggaacactt gtcgctgccg 900 ctggcagaca cagttgtccc cgaggaagct caggacctca ttcgtgggct gctgtgtcct 960 gctgagataa ggctaggtcg aggtggggca ggtgatttcc agaaacatcc tttcttcttt 1020 ggccttgatt gggagggtct ccgagacagt gtacccccct ttacaccaga cttcgagggt 1080 gccacggaca catgcaattt cgatgtggtg gaggaccggc tcactgccat ggtgagcggg 1140 ggcggggaga cgctgtcaga catgcaggaa gacatgcccc ttggggtgcg cctgcccttc 1200 gtgggctact cctactgctg catggccttc agagacaatc aggtcccgga ccccacccct 1260 atggaactag aggccctgca gttgcctgtg tcagacttgc aagggcttga cttgcagccc 1320 ccagtgtccc caccggatca agtggctgaa gaggccgacc tagtggctgt ccctgcccct 1380 gtggctgagg cagagaccac ggtaacgctg cagcagctcc aggaagccct ggaagaagag 1440 gttctcaccc ggcagagcct gagccgcgag ctggaggcca tccggaccgc caaccagaac 1500 ttctccagcc aactacagga ggccgaggtc cgaaaccgag acctggaggc gcatgttcgg 1560 cagctacagg aacggatgga gatgctgcag gccccaggag ccgcagccat cacgggggtc 1620 cccagtcccc gggccacgga tccaccttcc catctagatg gccccccggc cgtggctgtg 1680 ggccagtgcc cgctggtggg gccaggcccc atgcaccgcc gtcacctgct gctccctgcc 1740 aggatcccta ggcctggcct atccgaggcg cgttgcctgc tcctgttcgc cgctgctctg 1800 gctgctgccg ccacactggg ctgcactggg ttggtggcct ataccggcgg tctcacccca 1860 gtctggtgtt tcccgggagc caccttcgcc ccctga 1896 <210> SEQ ID NO 5 <211> LENGTH: 771 <212> TYPE: DNA <213> ORGANISM: mus musculus <220> FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION: (89)..(89) <223> OTHER INFORMATION: n is a, c, g, or t <220> FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION: (238)..(238) <223> OTHER INFORMATION: n is a, c, g, or t <220> FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION: (506)..(506) <223> OTHER INFORMATION: n is a, c, g, or t <400> SEQUENCE: 5 cctgcccctg tggctgaggc agagaccacg gtaacgctgc agcagctcca ggaagccctg 60 gaagaagagg ttctcacccg gcagagctng agccgcgagc tggaggccat ccggaccgcc 120 aaccagaact tctccagcca actacaggag gccgaggtcc gaaaccgaga cctggaggcg 180 catgttcggc agctacagga acggatggag atgctgcagg ccccaggagc cgccggantc 240 cctcacctgc ttccagccaa gggggcactg ggtggagatg gggggcatgt tgggtgtgtg 300 aaccctcggg gcaggggagg agtccaggct ggggcaccgc gccgcgccac tgcctttctc 360 ctccatcctc cacactccat acacctctct cttctccttc cagccatcac gggggtccca 420 gtccccgggc cacggatcca ccttcccatc tagatggccc cccggcggtg gctgtgggcc 480 agtgcccgct ggtggggcca ggacantgtc accgccgtca cctgctgctc cctgccagga 540 ttcctaggcc tggctatccg aggcgcgttg ctgctcctgt tcgccgctgc tctggctgct 600 gcgccacact gggctgcact gggttggttg gctataccgg cggtcttcac ccagtctggt 660 gtttcccgtg agcacccttc gcccctgaaa cctaagactt caagccatct ttcatttagg 720 ccttctagga aggtcgagcg acaggggagc gacccaaagc gtctctgtgc c 771 <210> SEQ ID NO 6 <211> LENGTH: 434 <212> TYPE: DNA <213> ORGANISM: mus musculus <400> SEQUENCE: 6 gagagaccca aggggtagtc agggacgggc agacatgcag ctagggttct ggggcctgga 60 caggggcagc caggccctgt gacgggaaga ccccgagctc cggcccgggg aggggccatg 120 gtgttgcctg cccaacatgt cagccgaagt gcggctgagg cagctccagc agctggtgct 180 ggacccaggc ttcctgggac tggagcccct gctcgacctt ctcctgggcg tccaccagga 240 gctgggtgcc tctcacctag cccaggacaa gtatgtggcc gacttcttgc agtgggtgga 300 gcccattgca gcaaggctta aggaggtccg actgcagagg gatgattttg agattttgaa 360 ggtgatcggg cgtggggcgt tcagcgaggt agcggtggtg aagatgaaac acacggagtc 420 tttggcttcg gaca 434 <210> SEQ ID NO 7 <211> LENGTH: 2688 <212> TYPE: DNA <213> ORGANISM: mus musculus

<400> SEQUENCE: 7 ccacgcgtcc gcccacgcgt ccggggcaga catgcagcta gggttctggg gcctggacag 60 gggcagccag gccctgtgac gggaagaccc cgagctccgg cccggggagg ggccatggtg 120 ttgcctgccc aacatgtcag ccgaagtgcg gctgaggcag ctccagcagc tggtgctgga 180 cccaggcttc ctgggactgg agcccctgct cgaccttctc ctgggcgtcc accaggagct 240 gggtgcctct cacctagccc aggacaagta tgtggccgac ttcttgcagt gggtggagcc 300 cattgcagca aggcttaagg aggtccgact gcagagggat gattttgaga ttttgaaggt 360 gatcgggcgt ggggcgttca gcgaggtagc ggtggtgaag atgaaacaga cgggccaagt 420 gtatgccatg aagattatga ataagtggga catgctgaag agaggcgagg tgtcgtgctt 480 ccgggaagaa agggatgtat tagtgaaagg ggaccggcgc tggatcacac agctgcactt 540 tgccttccag gatgagaact acctgtacct ggtcatggaa tactacgtgg gcggggacct 600 gctaacgctg ctgagcaagt ttggggagcg gatccccgcc gagatggctc gcttctacct 660 ggccgagatt gtcatggcca tagactccgt gcaccggctg ggctacgtgc acagggacat 720 caaaccagat aacattctgc tggaccgatg tgggcacatt cgcctggcag acttcggctc 780 ctgcctcaaa ctgcagcctg atggaatggt gaggtcgctg gtggctgtgg gcaccccgga 840 ctacctgtct cctgagattc tgcaggccgt tggtggaggg cctggggcag gcagctacgg 900 gccagagtgt gactggtggg cactgggcgt gttcacctat gagatgttct atgggcagac 960 ccccttctac gcggactcca cagccgagac atatgccaag attgtgcact acagggaaca 1020 cttgtcgctg ccgctggcag acacagttgt ccccgaggaa gctcaggacc tcattcgtgg 1080 gctgctgtgt cctgctgaga taaggctagg tcgaggtggg gcaggtgatt tccagaaaca 1140 tcctttcttc tttggccttg attgggaggg tctccgagac agtgtacccc cctttacacc 1200 agacttcgag ggtgccacgg acacatgcaa tttcgatgtg gtggaggacc ggctcactgc 1260 catggtgagc gggggcgggg agacgctgtc agacatgcag gaagacatgc cccttggggt 1320 gcgcctgccc ttcgtgggct actcctactg ctgcatggcc ttcagagaca atcaggtccc 1380 ggaccccacc cctatggaac tagaggccct gcagttgcct gtgtcagact tgcaagggct 1440 tgacttgcag cccccagtgt ccccaccgga tcaagtggct gaagaggctg acctagtggc 1500 tgtccctgcc cctgtggctg aggcagagac cacggtaacg ctgcagcagc tccaggaagc 1560 cctggaagaa gaggttctca cccggcagag cctgagccgc gagctggagg ccatccggac 1620 cgccaaccag aacttctcca gccaactaca ggaggccgag gtccgaaacc gagacctgga 1680 ggcgcatgtt cggcagctac aggaacggat ggagatgctg caggccccag gagccgcaga 1740 tccctaggcc tggcctatcc gaggcgcgtt gcctgctcct gttcgccgct gctctggctg 1800 ctgccgccac actgggctgc actgggttgg tggcctatac cggcggtctc accccagtct 1860 ggtgtttccc gggagccacc ttcgccccct gaaccctaag actccaagcc atctttcatt 1920 taggcctcct aggaaggtcg agcgaccagg gagcgaccca aagcgtctct gtgcccatcg 1980 cccccccccc cccccccacc gctccgctcc acacttctgt gagcctgggt ccccacccag 2040 ctccgctcct gtgatccagg cctgccacct ggcggccggg gagggaggaa cagggctcgt 2100 gcccagcacc cctggttcct gcagagctgg tagccaccgc tgctgcagca gctgggcatt 2160 cgccgacctt gctttactca gccccgacgt ggatgggcaa actgctcagc tcatccgatt 2220 tcactttttc actctcccag ccatcagtta caagccataa gcatgagccc cctatttcca 2280 gggacatccc attcccatag tgatggatca gcaagacctc tgccagcaca cacggagtct 2340 ttggcttcgg acagcctcac tcctgggggt tgctgcaact ccttccccgt gtacacgtct 2400 gcactctaac aacggagcca cagctgcact cccccctccc ccaaagcagt gtgggtattt 2460 attgatcttg ttatctgact cactgacaga ctccgggacc cacgttttag atgcattgag 2520 actcgacatt cctcggtatt tattgtctgt ccccacctac gacctccact cccgaccctt 2580 gcgaataaaa tacttctggt ctgccctaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa 2640 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaa 2688 <210> SEQ ID NO 8 <211> LENGTH: 2862 <212> TYPE: DNA <213> ORGANISM: mus musculus <400> SEQUENCE: 8 gggatagtca gggacgggca gacatgcagc tagggttctg gggcctggac aggggcagcc 60 aggccctgtg acgggaagac cccgagctcc ggcccgggga ggggccatgg tgttgcctgc 120 ccaacatgtc agccgaagtg cggctgaggc agctccagca gctggtgctg gacccaggct 180 tcctgggact ggagcccctg ctcgaccttc tcctgggcgt ccaccaggag ctgggtgcct 240 ctcacctagc ccaggacaag tatgtggccg acttcttgca gtgggtggag cccattgcag 300 caaggcttaa ggaggtccga ctgcagaggg atgattttga gattttgaag gtgatcgggc 360 gtggggcgtt cagcgaggta gcggtggtga agatgaaaca gacgggccaa gtgtatgcca 420 tgaagattac gaataagtgg gacatgctga agagaggcga ggtgtcgtgc ttccgggaag 480 aaagggatgt attagtgaaa ggggaccggc gctggatcac acagctgcac tttgccttcc 540 aggatgagaa ctacctgtac ctggtcatgg aatactacgt gggcggggac ctgctaacgc 600 tgctgagcaa gtttggggag cggatccccg ccgagatggc tcgcttctac ctggccgaga 660 ttgtcatggc catagactcc gtgcaccggc tgggctacgt gcacagggac atcaaaccag 720 ataacattct gctggaccga tgtgggcaca ttcgcctggc agacttcggc tcctgcctca 780 aactgcagcc tgatggaatg gtgaggtcgc tggtggctgt gggcaccccg gactacctgt 840 ctcctgagat tctgcaggcc gttggtggag ggcctggggc aggcagctac gggccagagt 900 gtgactggtg ggcactgggc gtgttcacct atgagatgtt ctatgggcag acccccttct 960 acgcggactc cacagccgag acatatgcca agattgtgca ctacagggaa cacttgtcgc 1020 tgccgctggc agacacagtt gtccccgagg aagctcagga cctcattcgt gggctgctgt 1080 gtcctgctga gataaggcta ggtcgaggtg gggcaggtga tttccagaaa catcctttct 1140 tctttggcct tgattgggag ggtctccgag acagtgtacc cccctttaca ccagacttcg 1200 agggtgccac ggacacatgc aatttcgatg tggtggagga ccggctcact gccatggaga 1260 cgctgtcaga catgcaggaa gacatgcccc ttggggtgcg cctgcccttc gtgggctact 1320 cctactgctg catggccttc agagacaatc aggtcccgga ccccacccct atggaactag 1380 aggccctgca gttgcctgtg tcagacttgc aagggcttga cttgcagccc ccagtgtccc 1440 caccggatca agtggctgaa gaggctgacc tagtggctgt ccctgcccct gtggctgagg 1500 cagagaccac ggtaacgctg cagcagctcc aggaagccct ggaagaagag gttctcaccc 1560 ggcagagcct gagccgcgag ctggaggcca tccggaccgc caaccagaac ttctccagcc 1620 aactacagga ggccgaggtc cgaaaccgag acctggaggc gcatgttcgg cagctacagg 1680 aacggatgga gatgctgcag gccccaggag ccgcagccat cacgggggtc cccagtcccc 1740 gggccacgga tccaccttcc catgcttctc gccaaatcct ccccaaggga actccctaga 1800 ctcccgttct ggcctcgact agattcccgc actgcctctc gccctgctgc tgggctccga 1860 tcgggtcacc tgtcccttct ctctccagct agatggcccc ccggccgtgg ctgtgggcca 1920 gtgcccgctg gtggggccag gccccatgca ccgccgtcac ctgctgctcc ctgccaggat 1980 ccctaggcct ggcctatccg aggcgcgttg cctgctcctg ttcgccgctg ctctggctgc 2040 tgccgccaca ctgggctgca ctgggttggt ggcctatacc ggcggtctca ccccagtctg 2100 gtgtttcccg ggagccacct tcgccccctg aaccctaaga ctccaagcca tctttcattt 2160 aggcctccta ggaaggtcga gcgaccaggg agcgacccaa agcgtctctg tgcccatcgc 2220 cccccccccc ccccccaccg ctccgctcca cacttctgtg agcctgggtc cccacccagc 2280 tccgctcctg tgatccaggc ctgccacctg gcggccgggg agggaggaac agggctcgtg 2340 cccagcaccc ctggttcctg cagagctggt agccaccgct gctgcagcag ctgggcattc 2400 gccgaccttg ctttactcag ccccgacgtg gatgggcaaa ctgctcagct catccgattt 2460 cactttttca ctctcccagc catcagttac aagccataag catgagcccc ctatttccag 2520 ggacatccca ttcccatagt gatggatcag caagacctct gccagcacac acggagtctt 2580 tggcttcgga cagcctcact cctgggggtt gctgcaactc cttccccgtg tacacgtctg 2640 cactctaaca acggagccac agctgcactc ccccctcccc caaagcagtg tgggtattta 2700 ttgatcttgt tatctgactc actgacagac tccgggaccc acgttttaga tgcattgaga 2760 ctcgacattc ctcggtattt attgtctgtc cccacctacg acctccactc ccgacccttg 2820 cgaataaaat acttctggtc tgccctaaaa aaaaaaaaaa aa 2862 <210> SEQ ID NO 9 <211> LENGTH: 2611 <212> TYPE: DNA <213> ORGANISM: mus musculus <400> SEQUENCE: 9 cgggaagacc ccgagctccg gcccggggag gggccatggt gttgcctgcc caacatgtca 60 gccgaagtgc ggctgaggca gctccagcag ctggtgctgg acccaggctt cctgggactg 120 gagcccctgc tcgaccttct cctgggcgtc caccaggagc tgggtgcctc tcacctagcc 180 caggacaagt atgtggccga cttcttgcag tgggtggagc ccattgcagc aaggcttaag 240 gaggtccgac tgcagaggga tgattttgag attttgaagg tgatcgggcg tggggcgttc 300 agcgaggtag cggtggtgaa gatgaaacag acgggccaag tgtatgccat gaagattatg 360 aataagtggg acatgctgaa gagaggcgag gtgtcgtgct tccgggaaga aagggatgta 420 ttagtgaaag gggaccggcg ctggatcaca cagctgcact ttgccttcca ggatgagaac 480 tacctgtacc tggtcatgga atactacgtg ggcggggacc tgctaacgct gctgagcaag 540 tttggggagc ggatccccgc cgagatggct cgcttctacc tggccgagat tgtcatggcc 600 atagactccg tgcaccggct gggctacgtg cacagggaca tcaaaccaga taacattctg 660 ctggaccgat gtgggcacat tcgcctggca gacttcggct cctgcctcaa actgcagcct 720 gatggaatgg tgaggtcgct ggtggctgtg ggcaccccgg actacctgtc tcctgagatt 780 ctgcaggccg ttggtggagg gcctggggca ggcagctacg ggccagagtg tgactggtgg 840 gcactgggcg tgttcaccta tgagatgttc tatgggcaga cccccttcta cgcggactcc 900 acagccgaga catatgccaa gattgtgcac tacagggaac acttgtcgct gccgctggca 960 gacacagttg tccccgagga agctcaggac ctcattcgtg ggctgctgtg tcctgctgag 1020 ataaggctag gtcgaggtgg ggcagacttc gagggtgcca cggacacatg caatttcgat 1080 gtggtggagg accggctcac tgccatggtg agcgggggcg gggagacgct gtcagacatg 1140 caggaagaca tgccccttgg ggtgcgcctg cccttcgtgg gctactccta ctgctgcatg 1200 gccttcagag acaatcaggt cccggacccc acccctatgg aactagaggc cctgcagttg 1260 cctgtgtcag acttgcaagg gcttgacttg cagcccccag tgtccccacc ggatcaagtg 1320 gctgaagagg ctgacctagt ggctgtccct gcccctgtgg ctgaggcaga gaccacggta 1380

acgctgcagc agctccagga agccctggaa gaagaggttc tcacccggca gagcctgagc 1440 cgcgagctgg aggccatccg gaccgccaac cagaacttct ccagccaact acaggaggcc 1500 gaggtccgaa accgagacct ggaggcgcat gttcggcagc tacaggaacg gatggagatg 1560 ctgcaggccc caggagccgc agccatcacg ggggtcccca gtccccgggc cacggatcca 1620 ccttcccatc tagatggccc cccggccgtg gctgtgggcc agtgcccgct ggtggggcca 1680 ggccccatgc accgccgtca cctgctgctc cctgccagga tccctaggcc tggcctatcc 1740 gaggcgcgtt gcctgctcct gttcgccgct gctctggctg ctgccgccac actgggctgc 1800 actgggttgg tggcctatac cggcggtctc accccagtct ggtgtttccc gggagccacc 1860 ttcgccccct gaaccctaag actccaagcc atctttcatt taggcctcct aggaaggtcg 1920 agcgaccagg gagcgaccca aagcgtctct gtgcccatcg cccccccccc cccccccacc 1980 gctccgctcc acacttctgt gagcctgggt ccccacccag ctccgctcct gtgatccagg 2040 cctgccacct ggcggccggg gagggaggaa cagggctcgt gcccagcacc cctggttcct 2100 gcagagctgg tagccaccgc tgctgcagca gctgggcatt cgccgacctt gctttactca 2160 gccccgacgt ggatgggcaa actgctcagc tcatccgatt tcactttttc actctcccag 2220 ccatcagtta caagccataa gcatgagccc cctatttcca gggacatccc attcccatag 2280 tgatggatca gcaagacctc tgccagcaca cacggagtct ttggcttcgg acagcctcac 2340 tcctgggggt tgctgcaact ccttccccgt gtacacgtct gcactctaac aacggagcca 2400 cagctgcact cccccctccc ccaaagcagt gtgggtattt attgatcttg ttatctgact 2460 cactgacaga ctccgggacc cacgttttag atgcattgag actcgacatt cctcggtatt 2520 tattgtctgt ccccacctac gacctccact cccgaccctt gcgaataaaa tacttctggt 2580 ctgccctaaa aaaaaaaaaa aaaaaaaaaa a 2611 <210> SEQ ID NO 10 <211> LENGTH: 988 <212> TYPE: DNA <213> ORGANISM: mus musculus <220> FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION: (531)..(531) <223> OTHER INFORMATION: n is a, c, g, or t <220> FEATURE: <221> NAME/KEY: misc_feature <222> LOCATION: (942)..(942) <223> OTHER INFORMATION: n is a, c, g, or t <400> SEQUENCE: 10 gctggaccgg tccggaattc tccggatcgc cagcctttgt gggccatatt cgtcatccct 60 cctggcttct catctgcttt tgtggtccta gctcaagacc tctaattcct ctgctgactt 120 aaatgccctt ccccagaggt cttctcaggc ctagtggaca agcttggagc cttatctgct 180 cctgcccaac attgagccaa agctccagct taccccagct tccttacaat ggaccccatt 240 gcagcaaggc ttaaggaggt ccgactgcag agggatgatt ttgagatttt gaaggtgatc 300 gggcgtgggg cgttcagcga ggtagcggtg gtgaagatga aacagacggg ccaagtgtat 360 gccatgaaga ttatgaataa gtgggacatg ctgaagagag gcgaggtgtc gtgcttccgg 420 gaagaaaggg atgtattagt gaaaggggac cggcgctgga tcacacagct gcactttgcc 480 ttccaggatg agaactacct gtacctggtc atggaatact acgtgggcgg ngacctgcta 540 acgctgctga gcaagttttg gggagcggat ccccgccgag atggctcgct tctacctggc 600 cgagattgtc atggccatag actccgtgca ccggctgggc tacgtgcaca gggacatcaa 660 accagataac attctgctgg accgatgtgg gcacattcgc ctggcagact tcggctcctg 720 gcctcaactg cagcctgatg gaatggtgga gtcccctggt ggctgtgggc acccccggac 780 tacctgtctc ctgaaattct gcagggcctt ggtgggaggc cctggggaag gcaactacgg 840 gccaaaagtt ggaagggggg ggcctggggg gggttcccct atgaaaagtt ctatggggag 900 gacccccttt aagcggaatc ccaggccgaa aaatatgccc angattgggc cctaacaggg 960 aaaacttttc ccctgcccct gggacaat 988 <210> SEQ ID NO 11 <211> LENGTH: 649 <212> TYPE: DNA <213> ORGANISM: mus musculus <400> SEQUENCE: 11 ggcgtgttcg cctatgagat gttctatggg cagaccccct tctacgcgga ctccacagcc 60 gagacatatg ccaagattgt gcactacagg gaacacttgt cgctgccgct ggcagacaca 120 gttgtccccg aggaagctca ggacctcatt cgtgggctgc tgtgtcctgc tgagataagg 180 ctaggtcgag gtggggcagg tgatttccag aaacatcctt tcttctttgg ccttgattgg 240 gagggtctcc gagacagtgt accccccttt acaccagact tcgagggtgc cacggacaca 300 tgcaatttcg atgtggtgga ggaccggctc actgccatgg agacgctgtc agacatgcag 360 gaagacatgc cccttggggt gcgcctgccc ttcgtgggct actcctactg ctgcatggcc 420 ttcagagaca atcaggtccc ggaccccacc cctatggaac tagaggccct gcagttgcct 480 gtgtcagact tgcaagggct tgacttgcag cccccagtgt ccccaccgga tcaagtggtc 540 ccaactctga tccccaccga caggctgaag aggctgacct agtggctgtc cctgcccctg 600 tggctgaggc agagccacgg taacgctgca gcagctccag gaagccctg 649 <210> SEQ ID NO 12 <211> LENGTH: 527 <212> TYPE: DNA <213> ORGANISM: mus musculus <400> SEQUENCE: 12 atttcgatgt ggtggaggac cggctcactg ccatggtgag cgggggcggg gagacgctgt 60 cagacatgca ggaagacatg ccccttgggg tgcgcctgcc cttcgtgggc tactcctact 120 gctgcatggc cttcagagac aatcaggtcc cggaccccac ccctatggaa ctagaggccc 180 tgcagttgcc tgtgtcagac ttgcaagggc ttgacttgca gcccccagtg tccccaccgg 240 atcaagtggc tgaagaggct gacctagtgg ctgtccctgc ccctgtggct gaggcagaga 300 ccacggtaac gctgcagcag ctccaggaag ccctggaaga agaggttctc acccggcaga 360 gcctgagccg cgagctggag gccatccgga ccgccaacca gaacttctcc aggaggccga 420 ggtccgaaac cgagacctgg aggcgcatgt tcggcagcta caggaacgga tggagatgct 480 gcaggcccca ggaaccgcag ccatcacggg ggtccccagt cccccgg 527 <210> SEQ ID NO 13 <211> LENGTH: 567 <212> TYPE: DNA <213> ORGANISM: mus musculus <400> SEQUENCE: 13 atggtgaggt cgctggtggc tgtgggcacc ccggactacc tgtctcctga gattctgcag 60 gccgttggtg gagggcctgg ggcaggcagc tacgggccag agtgtgactg gtgggcactg 120 ggcgtgttcg cctatgagat gttctatggg cagaccccct tctacgcgga ctccacagcc 180 gagacatatg ccaagattgt gcactacagg gaacacttgt cgctgccgct ggcagacaca 240 gttgtccccg aggaagctca ggacctcatt cgtgggctgc tgtgtcctgc tgagataagg 300 ctaggtcgag gtggggcagg tgatttccag aaacatcctt tcttctttgg ccttgattgg 360 gagggtctcc gagacagtgt accccccttt acaccagact tcgagggtgc cacggacaca 420 tgcaatttcg atgtggtgga ggaccggctc actgccatgg tgagcggggg cggggtatga 480 ggacacacag gtgaccagtc cccaagacag tgagtgaggc ttcactcttg gcagtactaa 540 aattgaatgt agggggctgg gctcttg 567 <210> SEQ ID NO 14 <211> LENGTH: 2474 <212> TYPE: DNA <213> ORGANISM: mus musculus <400> SEQUENCE: 14 ccgggaagaa agggatgtat tagtgaaagg ggaccggcgc tggatcacac agctgcactt 60 tgccttccag gatgagaact acctgtacct ggtcatggaa tactacgtgg gcggggacct 120 gctaacgctg ctgagcaagt ttggggagcg gatccccgcc gagatggctc gcttctacct 180 ggccgagatt gtcatggcca tagactccgt gcaccggctg ggctacgtgc acagggacat 240 caaaccagat aacattctgc tggaccgatg tgggcacatt cgcctggcag acttcggctc 300 ctgcctcaaa ctgcagcctg atggaatggt gaggtcgctg gtggctgtgg gcaccccgga 360 ctacctgtct cctgagattc tgcaggccgt tggtggaggg cctggggcag gcagctacgg 420 gccagagtgt gactggtggg cactgggcgt gttcgcctat gagatgttct atgggcagac 480 ccccttctac gcggactcca cagccgagac atatgccaag attgtgcact acagggaaca 540 cttgtcgctg ccgctggcag acacagttgt ccccgaggaa gctcaggacc tcattcgtgg 600 gctgctgtgt cctgctgaga taaggctagg tcgagacttc gagggtgcca cggacacatg 660 caatttcgat gtggtggagg accggctcac tgccatggtg agcgggggcg gggagacgct 720 gtcagacatg caggaagaca tgccccttgg ggtgcgcctg cccttcgtgg gctactccta 780 ctgctgcatg gccttcagag acaatcaggt cccggacccc acccctatgg aactagaggc 840 cctgcagttg cctgtgtcag acttgcaagg gcttgacttg cagcccccag tgtccccacc 900 ggatcaagtg gctgaagagg ccgacctagt ggctgtccct gcccctgtgg ctgaggcaga 960 gaccacggta acgctgcagc agctccagga agccctggaa gaagaggttc tcacccggca 1020 gagcctgagc cgcgagctgg aggccatccg gaccgccaac cagaacttct ccagccaact 1080 acaggaggcc gaggtccgaa accgagacct ggaggcgcat gttcggcagc tacaggaacg 1140 gatggagatg ctgcaggccc caggagccgc aggcgagtcc ctcacctgct tccagccaag 1200 ggggcactgg gtggagatgg ggggcatgtt gggtgtgtga accctcgggg caggggagga 1260 gtccaggctg gggcaccgca gccgcgccac tgcctttctc ctccatcctc cacactccat 1320 acacctctct cttctccttc cagccatcac gggggtcccc agtccccggg ccacggatcc 1380 accttcccat gcttctcgcc aaatcctccc caagggaact ccctagactc ccgttctggc 1440 ctcgactaga ttcccgcact gcctctcgcc ctgctgctgg gctccgatcg ggtcacctgt 1500 cccttctctc tccagctaga tggccccccg gccgtggctg tgggccagtg cccgctggtg 1560 gggccaggcc ccatgcaccg ccgtcacctg ctgctccctg ccaggatccc taggcctggc 1620 ctatccgagg cgcgttgcct gctcctgttc gccgctgctc tggctgctgc cgccacactg 1680 ggctgcactg ggttggtggc ctataccggc ggtctcaccc cagtctggtg tttcccggga 1740 gccaccttcg ccccctgaac cctaagactc caagccatct ttcatttagg cctcctagga 1800 agatcgagcg accagggagc gacccaaagc gtctctgtgc ccatcgcccc cccccccccc 1860 cccaccgctc cgctccacac ttctgtgagc ctgggtcccc acccagctcc gctcctgtga 1920 tccaggcctg ccacctggcg gccggggagg gaggaacagg gctcgtgccc agcacccctg 1980 gttcctgcag agctggtagc caccgctgct gcagcagctg ggcattcgcc gaccttgctt 2040

tactcagccc tgacgtggat gggctaactg ctcagctcat ccgatttcac tttttcactc 2100 tcccagccat cagttacaag ccataagcat gagcccccta tttccaggga catcccattc 2160 ccatagtgat ggatcagcaa gacctctgcc agcacacacg gagtctttgg cttcggacag 2220 cctcactcct gggggttgct gcaactcctt ccccgtgtac acgtctgcac tctaacaacg 2280 gagccacagc tgcactcccc cctcccccaa agcagtgtgg gtatttattg atcttgttat 2340 ctgactcact gacagactcc gggacccacg ttttagatgc attgagactc gacattcctc 2400 ggtatttatt gtctgtcccc acctacgacc tccactcccg acccttgcga ataaaatact 2460 tctggtctgc ccta 2474 <210> SEQ ID NO 15 <211> LENGTH: 2135 <212> TYPE: DNA <213> ORGANISM: mus musculus <400> SEQUENCE: 15 ccgggaagaa agggatgtat tagtgaaagg ggaccggcgc tggatcacac agctgcactt 60 tgccttccag gatgagaact acctgtacct ggtcatggaa tactacgtgg gcggggacct 120 gctaacgctg ctgagcaagt ttggggagcg gatccccgcc gagatggctc gcttctacct 180 ggccgagatt gtcatggcca tagactccgt gcaccggctg ggctacgtgc acagggacat 240 caaaccagat aacattctgc tggaccgatg tgggcacatt cgcctggcag acttcggctc 300 ctgcctcaaa ctgcagcctg atggaatggt gaggtcgctg gtggctgtgg gcaccccgga 360 ctacctgtct cctgagattc tgcaggccgt tggtggaggg cctggggcag gcagctacgg 420 gccagagtgt gactggtggg cactgggcgt gttcgcctat gagatgttct atgggcagac 480 ccccttctac gcggactcca cagccgagac atatgccaag attgtgcact acagggaaca 540 cttgtcgctg ccgctggcag acacagttgt ccccgaggaa gctcaggacc tcattcgtgg 600 gctgctgtgt cctgctgaga taaggctagg tcgaggtggg gcaggtgatt tccagaaaca 660 tcctttcttc tttggccttg attgggaggg tctccgagac agtgtacccc cctttacacc 720 agacttcgag ggtgccacgg acacatgcaa tttcgatgtg gtggaggacc ggctcactgc 780 catggagacg ctgtcagaca tgcaggaaga catgcccctt ggggtgcgcc tgcccttcgt 840 gggctactcc tactgctgca tggccttcag agctgaagag gccgacctag tggctgtccc 900 tgcccctgtg gctgaggcag agaccacggt aacgctgcag cagctccagg aagccctgga 960 agaagaggtt ctcacccggc agagcctgag ccgcgagctg gaggccatcc ggaccgccaa 1020 ccagaacttc tccagccaac tacaggaggc cgaggtccga aaccgagacc tggaggcgca 1080 tgttcggcag ctacaggaac ggatggagat gctgcaggcc ccaggagccg cagccatcac 1140 gggggtcccc agtccccggg ccacggatcc accttcccat atggcccccc ggccgtggct 1200 gtgggccagt gcccgctggt ggggccaggc cccatgcacc gccgtcacct gctgctccct 1260 gccaggatcc ctaggcctgg cctatccgag gcgcgttgcc tgctcctgtt cgccgctgct 1320 ctggctgctg ccgccacact gggctgcact gggttggtgg cctataccgg cggtctcacc 1380 ccagtctggt gtttcccggg agccaccttc gccccctgaa ccctaagact ccaagccatc 1440 tttcatttag gcctcctagg aagatcgagc gaccagggag cgacccaaag cgtctctgtg 1500 cccatcgccc cccccccccc ccccaccgct ccgctccaca cttctgtgag cctgggtccc 1560 cacccagctc cgctcctgtg atccaggcct gccacctggc ggccggggag ggaggaacag 1620 ggctcgtgcc cagcacccct ggttcctgca gagctggtag ccaccgctgc tgcagcagct 1680 gggcattcgc cgaccttgct ttactcagcc ctgacgtgga tgggctaact gctcagctca 1740 tccgatttca ctttttcact ctcccagcca tcagttacaa gccataagca tgagccccct 1800 atttccaggg acatcccatt cccatagtga tggatcagca agacctctgc cagcacacac 1860 ggagtctttg gcttcggaca gcctcactcc tgggggttgc tgcaactcct tccccgtgta 1920 cacgtctgca ctctaacaac ggagccacag ctgcactccc ccctccccca aagcagtgtg 1980 ggtatttatt gatcttgtta tctgactcac tgacagactc cgggacccac gttttagatg 2040 cattgagact cgacattcct cggtatttat tgtctgtccc cacctacgac ctccactccc 2100 gacccttgcg aataaaatac ttctggtctg cccta 2135 <210> SEQ ID NO 16 <211> LENGTH: 2873 <212> TYPE: DNA <213> ORGANISM: homo sapiens <400> SEQUENCE: 16 aggggggctg gaccaagggg tggggagaag gggaggaggc ctcggccggc cgcagagaga 60 agtggccaga gaggcccagg ggacagccag ggacaggcag acatgcagcc agggctccag 120 ggcctggaca ggggctgcca ggccctgtga caggaggacc ccgagccccc ggcccgggga 180 ggggccatgg tgctgcctgt ccaacatgtc agccgaggtg cggctgaggc ggctccagca 240 gctggtgttg gacccgggct tcctggggct ggagcccctg ctcgaccttc tcctgggcgt 300 ccaccaggag ctgggcgcct ccgaactggc ccaggacaag tacgtggccg acttcttgca 360 gtgggcggag cccatcgtgg tgaggcttaa ggaggtccga ctgcagaggg acgacttcga 420 gattctgaag gtgatcggac gcggggcgtt cagcgaggta gcggtagtga agatgaagca 480 gacgggccag gtgtatgcca tgaagatcat gaacaagtgg gacatgctga agaggggcga 540 ggtgtcgtgc ttccgtgagg agagggacgt gttggtgaat ggggaccggc ggtggatcac 600 gcagctgcac ttcgccttcc aggatgagaa ctacctgtac ctggtcatgg agtattacgt 660 gggcggggac ctgctgacac tgctgagcaa gtttggggag cggattccgg ccgagatggc 720 gcgcttctac ctggcggaga ttgtcatggc catagactcg gtgcaccggc ttggctacgt 780 gcacagggac atcaaacccg acaacatcct gctggaccgc tgtggccaca tccgcctggc 840 cgacttcggc tcttgcctca agctgcgggc agatggaacg gtgcggtcgc tggtggctgt 900 gggcacccca gactacctgt cccccgagat cctgcaggct gtgggcggtg ggcctgggac 960 aggcagctac gggcccgagt gtgactggtg ggcgctgggt gtattcgcct atgaaatgtt 1020 ctatgggcag acgcccttct acgcggattc cacggcggag acctatggca agatcgtcca 1080 ctacaaggag cacctctctc tgccgctggt ggacgaaggg gtccctgagg aggctcgaga 1140 cttcattcag cggttgctgt gtcccccgga gacacggctg ggccggggtg gagcaggcga 1200 cttccggaca catcccttct tctttggcct cgactgggat ggtctccggg acagcgtgcc 1260 cccctttaca ccggatttcg aaggtgccac cgacacatgc aacttcgact tggtggagga 1320 cgggctcact gccatggaga cactgtcgga cattcgggaa ggtgcgccgc taggggtcca 1380 cctgcctttt gtgggctact cctactcctg catggccctc agggacagtg aggtcccagg 1440 ccccacaccc atggaactgg aggccgagca gctgcttgag ccacacgtgc aagcgcccag 1500 cctggagccc tcggtgtccc cacaggatga aacagctgaa gtggcagttc cagcggctgt 1560 ccctgcggca gaggctgagg ccgaggtgac gctgcgggag ctccaggaag ccctggagga 1620 ggaggtgctc acccggcaga gcctgagccg ggagatggag gccatccgca cggacaacca 1680 gaacttcgcc agtcaactac gcgaggcaga ggctcggaac cgggacctag aggcacacgt 1740 ccggcagttg caggagcgga tggagttgct gcaggcagag ggagccacag ctgtcacggg 1800 ggtccccagt ccccgggcca cggatccacc ttcccatatg gccccccggc cgtggctgtg 1860 ggccagtgcc cgctggtggg gccaggcccc atgcaccgcc gccacctgct gctccctgcc 1920 agggtcccta ggcctggcct atcggaggcg ctttccctgc tcctgttcgc cgttgttctg 1980 tctcgtgccg ccgccctggg ctgcattggg ttggtggccc acgccggcca actcaccgca 2040 gtctggcgcc gcccaggagc cgcccgcgct ccctgaaccc tagaactgtc ttcgactccg 2100 gggccccgtt ggaagactga gtgcccgggg cacggcacag aagccgcgcc caccgcctgc 2160 cagttcacaa ccgctccgag cgtgggtctc cgcccagctc cagtcctgtg atccgggccc 2220 gccccctagc ggccggggag ggaggggccg ggtccgcggc cggcgaacgg ggctcgaagg 2280 gtccttgtag ccgggaatgc tgctgctgct gctgctgctg ctgctgctgc tgctgctgct 2340 gctgctgctg ctgctgctgg ggggatcaca gaccatttct ttctttcggc caggctgagg 2400 ccctgacgtg gatgggcaaa ctgcaggcct gggaaggcag caagccgggc cgtccgtgtt 2460 ccatcctcca cgcaccccca cctatcgttg gttcgcaaag tgcaaagctt tcttgtgcat 2520 gacgccctgc tctggggagc gtctggcgcg atctctgcct gcttactcgg gaaatttgct 2580 tttgccaaac ccgctttttc ggggatcccg cgcccccctc ctcacttgcg ctgctctcgg 2640 agccccagcc ggctccgccc gcttcggcgg tttggatatt tattgacctc gtcctccgac 2700 tcgctgacag gctacaggac ccccaacaac cccaatccac gttttggatg cactgagacc 2760 ccgacattcc tcggtattta ttgtctgtcc ccacctagga cccccacccc cgaccctcgc 2820 gaataaaagg ccctccatct gcccaaaaaa aaaaaaaaaa aaaaaaaaaa aaa 2873 <210> SEQ ID NO 17 <211> LENGTH: 1509 <212> TYPE: DNA <213> ORGANISM: homo sapiens <400> SEQUENCE: 17 ccaccgcagc ggacagcgcc aagtgaagcc tcgcttcccc tccgcggcga ccagggcccg 60 agccgagagt agcagttgta gctacccgcc cagaaactag acacaatgtg cgacgaagac 120 gagaccaccg ccctcgtgtg cgacaatggc tccggcctgg tgaaagccgg cttcgccggg 180 gatgacgccc ctagggccgt gttcccgtcc atcgtgggcc gcccccgaca ccagggcgtc 240 atggtcggta tgggtcagaa agattcctac gtgggcgacg aggctcagag caagagaggt 300 atcctgaccc tgaagtaccc tatcgagcac ggcatcatca ccaactggga tgacatggag 360 aagatctggc accacacctt ctacaacgag cttcgcgtgg ctcccgagga gcaccccacc 420 ctgctcaccg aggcccccct caatcccaag gccaaccgcg agaagatgac ccagatcatg 480 tttgagacct tcaacgtgcc cgccatgtac gtggccatcc aggccgtgct gtccctctac 540 gcctccggca ggaccaccgg catcgtgctg gactccggcg acggcgtcac ccacaacgtg 600 cccatttatg agggctacgc gctgccgcac gccatcatgc gcctggacct ggcgggccgc 660 gatctcaccg actacctgat gaagatcctc actgagcgtg gctactcctt cgtgaccaca 720 gctgagcgcg agatcgtgcg cgacatcaag gagaagctgt gctacgtggc cctggacttc 780 gagaacgaga tggcgacggc cgcctcctcc tcctccctgg aaaagagcta cgagctgcca 840 gacgggcagg tcatcaccat cggcaacgag cgcttccgct gcccggagac gctcttccag 900 ccctccttca tcggtatgga gtcggcgggc attcacgaga ccacctacaa cagcatcatg 960 aagtgtgaca tcgacatcag gaaggacctg tatgccaaca acgtcatgtc ggggggcacc 1020 acgatgtacc ctgggatcgc tgaccgcatg cagaaagaga tcaccgcgct ggcacccagc 1080 accatgaaga tcaagatcat cgccccgccg gagcgcaaat actcggtgtg gatcggcggc 1140 tccatcctgg cctcgctgtc caccttccag cagatgtgga tcaccaagca ggagtacgac 1200 gaggccggcc cttccatcgt ccaccgcaaa tgcttctaga cacactccac ctccagcacg 1260

cgacttctca ggacgacgaa tcttctcaat gggggggcgg ctgagctcca gccaccccgc 1320 agtcactttc tttgtaacaa cttccgttgc tgccatcgta aactgacaca gtgtttataa 1380 cgtgtacata cattaactta ttacctcatt ttgttatttt tcgaaacaaa gccctgtgga 1440 agaaaatgga aaacttgaag aagcattaaa gtcattctgt taagctgcgt aaaaaaaaaa 1500 aaaaaaaaa 1509 <210> SEQ ID NO 18 <400> SEQUENCE: 18 000 <210> SEQ ID NO 19 <400> SEQUENCE: 19 000 <210> SEQ ID NO 20 <211> LENGTH: 18 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Primer <400> SEQUENCE: 20 agcctgagcc gggagatg 18 <210> SEQ ID NO 21 <211> LENGTH: 21 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Primer <400> SEQUENCE: 21 gcgtagttga ctggcgaagt t 21 <210> SEQ ID NO 22 <211> LENGTH: 21 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Probe <400> SEQUENCE: 22 aggccatccg cacggacaac c 21 <210> SEQ ID NO 23 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 23 acaataaata ccgagg 16 <210> SEQ ID NO 24 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 24 cggagcggtt gtgaactggc 20 <210> SEQ ID NO 25 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 25 gcgcaccttc ccgaatgtcc 20 <210> SEQ ID NO 26 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 26 cccgaatgtc cgacag 16 <210> SEQ ID NO 27 <211> LENGTH: 17 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 27 cggagcggtt gtgaact 17 <210> SEQ ID NO 28 <211> LENGTH: 17 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 28 acaataaata ccgagga 17 <210> SEQ ID NO 29 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 29 gacaatctcc gccagg 16 <210> SEQ ID NO 30 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 30 tcccgaatgt ccgaca 16 <210> SEQ ID NO 31 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 31 tgtaatgttg tccagt 16 <210> SEQ ID NO 32 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 32 gtgtaatgtt gtccag 16 <210> SEQ ID NO 33 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 33 ttgcactttg cgaaccaacg 20 <210> SEQ ID NO 34 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 34 cgacacctcg cccctcttca 20 <210> SEQ ID NO 35 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 35 acgacacctc gcccctcttc 20 <210> SEQ ID NO 36 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 36 cacgacacct cgcccctctt 20 <210> SEQ ID NO 37 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 37 gcacgacacc tcgcccctct 20 <210> SEQ ID NO 38 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 38 agcacgacac ctcgcccctc 20

<210> SEQ ID NO 39 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 39 aagcacgaca cctcgcccct 20 <210> SEQ ID NO 40 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 40 gaagcacgac acctcgcccc 20 <210> SEQ ID NO 41 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 41 ggaagcacga cacctcgccc 20 <210> SEQ ID NO 42 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 42 cggaagcacg acacctcgcc 20 <210> SEQ ID NO 43 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 43 acggaagcac gacacctcgc 20 <210> SEQ ID NO 44 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 44 cacggaagca cgacacctcg 20 <210> SEQ ID NO 45 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 45 tcacggaagc acgacacctc 20 <210> SEQ ID NO 46 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 46 ctcacggaag cacgacacct 20 <210> SEQ ID NO 47 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 47 cctcacggaa gcacgacacc 20 <210> SEQ ID NO 48 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 48 tcctcacgga agcacgacac 20 <210> SEQ ID NO 49 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 49 ctcctcacgg aagcacgaca 20 <210> SEQ ID NO 50 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 50 tctcctcacg gaagcacgac 20 <210> SEQ ID NO 51 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 51 ctctcctcac ggaagcacga 20 <210> SEQ ID NO 52 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 52 cctctcctca cggaagcacg 20 <210> SEQ ID NO 53 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 53 ccctctcctc acggaagcac 20 <210> SEQ ID NO 54 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 54 tccctctcct cacggaagca 20 <210> SEQ ID NO 55 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 55 gtccctctcc tcacggaagc 20 <210> SEQ ID NO 56 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 56 cgtccctctc ctcacggaag 20 <210> SEQ ID NO 57 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 57 ggtccccatt caccaacacg 20 <210> SEQ ID NO 58 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 58 cggtccccat tcaccaacac 20 <210> SEQ ID NO 59 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 59

ccggtcccca ttcaccaaca 20 <210> SEQ ID NO 60 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 60 gccggtcccc attcaccaac 20 <210> SEQ ID NO 61 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 61 cgccggtccc cattcaccaa 20 <210> SEQ ID NO 62 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 62 ccgccggtcc ccattcacca 20 <210> SEQ ID NO 63 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 63 accgccggtc cccattcacc 20 <210> SEQ ID NO 64 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 64 caccgccggt ccccattcac 20 <210> SEQ ID NO 65 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 65 ccaccgccgg tccccattca 20 <210> SEQ ID NO 66 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 66 tccaccgccg gtccccattc 20 <210> SEQ ID NO 67 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 67 atccaccgcc ggtccccatt 20 <210> SEQ ID NO 68 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 68 gatccaccgc cggtccccat 20 <210> SEQ ID NO 69 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 69 tgatccaccg ccggtcccca 20 <210> SEQ ID NO 70 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 70 gtgatccacc gccggtcccc 20 <210> SEQ ID NO 71 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 71 cgtgatccac cgccggtccc 20 <210> SEQ ID NO 72 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 72 ttctcatcct ggaaggcgaa 20 <210> SEQ ID NO 73 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 73 gttctcatcc tggaaggcga 20 <210> SEQ ID NO 74 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 74 agttctcatc ctggaaggcg 20 <210> SEQ ID NO 75 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 75 gtagttctca tcctggaagg 20 <210> SEQ ID NO 76 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 76 ggtagttctc atcctggaag 20 <210> SEQ ID NO 77 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 77 aggtagttct catcctggaa 20 <210> SEQ ID NO 78 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 78 caggtagttc tcatcctgga 20 <210> SEQ ID NO 79 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 79 tacaggtagt tctcatcctg 20 <210> SEQ ID NO 80 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 80

gtacaggtag ttctcatcct 20 <210> SEQ ID NO 81 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 81 ggtacaggta gttctcatcc 20 <210> SEQ ID NO 82 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 82 aggtacaggt agttctcatc 20 <210> SEQ ID NO 83 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 83 ccaggtacag gtagttctca 20 <210> SEQ ID NO 84 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 84 accaggtaca ggtagttctc 20 <210> SEQ ID NO 85 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 85 gaccaggtac aggtagttct 20 <210> SEQ ID NO 86 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 86 tgaccaggta caggtagttc 20 <210> SEQ ID NO 87 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 87 catgaccagg tacaggtagt 20 <210> SEQ ID NO 88 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 88 ccatgaccag gtacaggtag 20 <210> SEQ ID NO 89 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 89 tccatgacca ggtacaggta 20 <210> SEQ ID NO 90 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 90 ctccatgacc aggtacaggt 20 <210> SEQ ID NO 91 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 91 actccatgac caggtacagg 20 <210> SEQ ID NO 92 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 92 tactccatga ccaggtacag 20 <210> SEQ ID NO 93 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 93 atactccatg accaggtaca 20 <210> SEQ ID NO 94 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 94 aatactccat gaccaggtac 20 <210> SEQ ID NO 95 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 95 taatactcca tgaccaggta 20 <210> SEQ ID NO 96 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 96 cgtaatactc catgaccagg 20 <210> SEQ ID NO 97 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 97 agcagtgtca gcaggtcccc 20 <210> SEQ ID NO 98 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 98 cagcagtgtc agcaggtccc 20 <210> SEQ ID NO 99 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 99 tcagcagtgt cagcaggtcc 20 <210> SEQ ID NO 100 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 100 ctcagcagtg tcagcaggtc 20 <210> SEQ ID NO 101 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide

<400> SEQUENCE: 101 gctcagcagt gtcagcaggt 20 <210> SEQ ID NO 102 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 102 tgctcagcag tgtcagcagg 20 <210> SEQ ID NO 103 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 103 ttgctcagca gtgtcagcag 20 <210> SEQ ID NO 104 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 104 cttgctcagc agtgtcagca 20 <210> SEQ ID NO 105 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 105 acttgctcag cagtgtcagc 20 <210> SEQ ID NO 106 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 106 aacttgctca gcagtgtcag 20 <210> SEQ ID NO 107 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 107 aaacttgctc agcagtgtca 20 <210> SEQ ID NO 108 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 108 caaacttgct cagcagtgtc 20 <210> SEQ ID NO 109 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 109 ccaaacttgc tcagcagtgt 20 <210> SEQ ID NO 110 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 110 cccaaacttg ctcagcagtg 20 <210> SEQ ID NO 111 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 111 gtgagcccgt cctccaccaa 20 <210> SEQ ID NO 112 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 112 agtgagcccg tcctccacca 20 <210> SEQ ID NO 113 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 113 cagtgagccc gtcctccacc 20 <210> SEQ ID NO 114 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 114 gcagtgagcc cgtcctccac 20 <210> SEQ ID NO 115 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 115 ggcagtgagc ccgtcctcca 20 <210> SEQ ID NO 116 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 116 tggcagtgag cccgtcctcc 20 <210> SEQ ID NO 117 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 117 catggcagtg agcccgtcct 20 <210> SEQ ID NO 118 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 118 ccatggcagt gagcccgtcc 20 <210> SEQ ID NO 119 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 119 tccatggcag tgagcccgtc 20 <210> SEQ ID NO 120 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 120 ctccatggca gtgagcccgt 20 <210> SEQ ID NO 121 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 121 tctccatggc agtgagcccg 20 <210> SEQ ID NO 122 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide

<400> SEQUENCE: 122 gtctccatgg cagtgagccc 20 <210> SEQ ID NO 123 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 123 ccttcccgaa tgtccgacag 20 <210> SEQ ID NO 124 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 124 accttcccga atgtccgaca 20 <210> SEQ ID NO 125 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 125 caccttcccg aatgtccgac 20 <210> SEQ ID NO 126 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 126 gcaccttccc gaatgtccga 20 <210> SEQ ID NO 127 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 127 cgcaccttcc cgaatgtccg 20 <210> SEQ ID NO 128 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 128 ggcgcacctt cccgaatgtc 20 <210> SEQ ID NO 129 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 129 acaaaaggca ggtggacccc 20 <210> SEQ ID NO 130 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 130 cacaaaaggc aggtggaccc 20 <210> SEQ ID NO 131 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 131 ccacaaaagg caggtggacc 20 <210> SEQ ID NO 132 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 132 cccacaaaag gcaggtggac 20 <210> SEQ ID NO 133 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 133 gcccacaaaa ggcaggtgga 20 <210> SEQ ID NO 134 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 134 agcccacaaa aggcaggtgg 20 <210> SEQ ID NO 135 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 135 tagcccacaa aaggcaggtg 20 <210> SEQ ID NO 136 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 136 gtagcccaca aaaggcaggt 20 <210> SEQ ID NO 137 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 137 agtagcccac aaaaggcagg 20 <210> SEQ ID NO 138 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 138 gagtagccca caaaaggcag 20 <210> SEQ ID NO 139 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 139 ggagtagccc acaaaaggca 20 <210> SEQ ID NO 140 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 140 aggagtagcc cacaaaaggc 20 <210> SEQ ID NO 141 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 141 taggagtagc ccacaaaagg 20 <210> SEQ ID NO 142 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 142 gtaggagtag cccacaaaag 20 <210> SEQ ID NO 143 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE:

<223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 143 agtaggagta gcccacaaaa 20 <210> SEQ ID NO 144 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 144 gagtaggagt agcccacaaa 20 <210> SEQ ID NO 145 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 145 ggagtaggag tagcccacaa 20 <210> SEQ ID NO 146 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 146 aggagtagga gtagcccaca 20 <210> SEQ ID NO 147 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 147 caggagtagg agtagcccac 20 <210> SEQ ID NO 148 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 148 gcaggagtag gagtagccca 20 <210> SEQ ID NO 149 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 149 tgcaggagta ggagtagccc 20 <210> SEQ ID NO 150 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 150 atgcaggagt aggagtagcc 20 <210> SEQ ID NO 151 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 151 catgcaggag taggagtagc 20 <210> SEQ ID NO 152 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 152 ccatgcagga gtaggagtag 20 <210> SEQ ID NO 153 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 153 gccatgcagg agtaggagta 20 <210> SEQ ID NO 154 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 154 ggccatgcag gagtaggagt 20 <210> SEQ ID NO 155 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 155 gggccatgca ggagtaggag 20 <210> SEQ ID NO 156 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 156 agggccatgc aggagtagga 20 <210> SEQ ID NO 157 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 157 gagggccatg caggagtagg 20 <210> SEQ ID NO 158 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 158 ctgagggcca tgcaggagta 20 <210> SEQ ID NO 159 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 159 cctgagggcc atgcaggagt 20 <210> SEQ ID NO 160 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 160 ccctgagggc catgcaggag 20 <210> SEQ ID NO 161 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 161 tccctgaggg ccatgcagga 20 <210> SEQ ID NO 162 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 162 gtccctgagg gccatgcagg 20 <210> SEQ ID NO 163 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 163 tgtccctgag ggccatgcag 20 <210> SEQ ID NO 164 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence

<220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 164 ctgtccctga gggccatgca 20 <210> SEQ ID NO 165 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 165 actgtccctg agggccatgc 20 <210> SEQ ID NO 166 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 166 cactgtccct gagggccatg 20 <210> SEQ ID NO 167 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 167 tcactgtccc tgagggccat 20 <210> SEQ ID NO 168 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 168 ctcactgtcc ctgagggcca 20 <210> SEQ ID NO 169 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 169 cctcactgtc cctgagggcc 20 <210> SEQ ID NO 170 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 170 acctcactgt ccctgagggc 20 <210> SEQ ID NO 171 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 171 gacctcactg tccctgaggg 20 <210> SEQ ID NO 172 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 172 ggacctcact gtccctgagg 20 <210> SEQ ID NO 173 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 173 gggacctcac tgtccctgag 20 <210> SEQ ID NO 174 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 174 cctccagttc catgggtgtg 20 <210> SEQ ID NO 175 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 175 gcctccagtt ccatgggtgt 20 <210> SEQ ID NO 176 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 176 ggcctccagt tccatgggtg 20 <210> SEQ ID NO 177 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 177 cggcctccag ttccatgggt 20 <210> SEQ ID NO 178 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 178 tcggcctcca gttccatggg 20 <210> SEQ ID NO 179 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 179 ctcggcctcc agttccatgg 20 <210> SEQ ID NO 180 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 180 gctcggcctc cagttccatg 20 <210> SEQ ID NO 181 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 181 tgctcggcct ccagttccat 20 <210> SEQ ID NO 182 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 182 ctgctcggcc tccagttcca 20 <210> SEQ ID NO 183 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 183 gctgctcggc ctccagttcc 20 <210> SEQ ID NO 184 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 184 agctgctcgg cctccagttc 20 <210> SEQ ID NO 185 <211> LENGTH: 20 <212> TYPE: DNA

<213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 185 cagctgctcg gcctccagtt 20 <210> SEQ ID NO 186 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 186 gcagctgctc ggcctccagt 20 <210> SEQ ID NO 187 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 187 agcagctgct cggcctccag 20 <210> SEQ ID NO 188 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 188 aagcagctgc tcggcctcca 20 <210> SEQ ID NO 189 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 189 caagcagctg ctcggcctcc 20 <210> SEQ ID NO 190 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 190 tcaagcagct gctcggcctc 20 <210> SEQ ID NO 191 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 191 ctcaagcagc tgctcggcct 20 <210> SEQ ID NO 192 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 192 gctcaagcag ctgctcggcc 20 <210> SEQ ID NO 193 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 193 ggctcaagca gctgctcggc 20 <210> SEQ ID NO 194 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 194 tggctcaagc agctgctcgg 20 <210> SEQ ID NO 195 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 195 gtggctcaag cagctgctcg 20 <210> SEQ ID NO 196 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 196 tgtggctcaa gcagctgctc 20 <210> SEQ ID NO 197 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 197 gtgtggctca agcagctgct 20 <210> SEQ ID NO 198 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 198 ccacttcagc tgtttcatcc 20 <210> SEQ ID NO 199 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 199 tgccacttca gctgtttcat 20 <210> SEQ ID NO 200 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 200 ctgccacttc agctgtttca 20 <210> SEQ ID NO 201 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 201 actgccactt cagctgtttc 20 <210> SEQ ID NO 202 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 202 aactgccact tcagctgttt 20 <210> SEQ ID NO 203 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 203 gaactgccac ttcagctgtt 20 <210> SEQ ID NO 204 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 204 ggaactgcca cttcagctgt 20 <210> SEQ ID NO 205 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 205 tggaactgcc acttcagctg 20 <210> SEQ ID NO 206 <211> LENGTH: 20

<212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 206 ctggaactgc cacttcagct 20 <210> SEQ ID NO 207 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 207 gctggaactg ccacttcagc 20 <210> SEQ ID NO 208 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 208 cgctggaact gccacttcag 20 <210> SEQ ID NO 209 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 209 ccgctggaac tgccacttca 20 <210> SEQ ID NO 210 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 210 gccgctggaa ctgccacttc 20 <210> SEQ ID NO 211 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 211 agccgctgga actgccactt 20 <210> SEQ ID NO 212 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 212 ctcagcctct gccgcaggga 20 <210> SEQ ID NO 213 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 213 cctcagcctc tgccgcaggg 20 <210> SEQ ID NO 214 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 214 ggcctcagcc tctgccgcag 20 <210> SEQ ID NO 215 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 215 cggcctcagc ctctgccgca 20 <210> SEQ ID NO 216 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 216 tcggcctcag cctctgccgc 20 <210> SEQ ID NO 217 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 217 ctcggcctca gcctctgccg 20 <210> SEQ ID NO 218 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 218 cctcggcctc agcctctgcc 20 <210> SEQ ID NO 219 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 219 acctcggcct cagcctctgc 20 <210> SEQ ID NO 220 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 220 cacctcggcc tcagcctctg 20 <210> SEQ ID NO 221 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 221 tcacctcggc ctcagcctct 20 <210> SEQ ID NO 222 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 222 gtcacctcgg cctcagcctc 20 <210> SEQ ID NO 223 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 223 cgtcacctcg gcctcagcct 20 <210> SEQ ID NO 224 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 224 agcacctcct cctccagggc 20 <210> SEQ ID NO 225 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 225 gagcacctcc tcctccaggg 20 <210> SEQ ID NO 226 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 226 tgagcacctc ctcctccagg 20 <210> SEQ ID NO 227

<211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 227 gtgagcacct cctcctccag 20 <210> SEQ ID NO 228 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 228 ggtgagcacc tcctcctcca 20 <210> SEQ ID NO 229 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 229 gggtgagcac ctcctcctcc 20 <210> SEQ ID NO 230 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 230 cgggtgagca cctcctcctc 20 <210> SEQ ID NO 231 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 231 ccgggtgagc acctcctcct 20 <210> SEQ ID NO 232 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 232 gccgggtgag cacctcctcc 20 <210> SEQ ID NO 233 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 233 tgccgggtga gcacctcctc 20 <210> SEQ ID NO 234 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 234 ctgccgggtg agcacctcct 20 <210> SEQ ID NO 235 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 235 tctgccgggt gagcacctcc 20 <210> SEQ ID NO 236 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 236 gctctgccgg gtgagcacct 20 <210> SEQ ID NO 237 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 237 ggctctgccg ggtgagcacc 20 <210> SEQ ID NO 238 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 238 aggctctgcc gggtgagcac 20 <210> SEQ ID NO 239 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 239 caggctctgc cgggtgagca 20 <210> SEQ ID NO 240 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 240 tcaggctctg ccgggtgagc 20 <210> SEQ ID NO 241 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 241 gctcaggctc tgccgggtga 20 <210> SEQ ID NO 242 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 242 cggctcaggc tctgccgggt 20 <210> SEQ ID NO 243 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 243 ccggctcagg ctctgccggg 20 <210> SEQ ID NO 244 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 244 cccggctcag gctctgccgg 20 <210> SEQ ID NO 245 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 245 tcccggctca ggctctgccg 20 <210> SEQ ID NO 246 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 246 ctcccggctc aggctctgcc 20 <210> SEQ ID NO 247 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 247 tctcccggct caggctctgc 20

<210> SEQ ID NO 248 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 248 atctcccggc tcaggctctg 20 <210> SEQ ID NO 249 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 249 catctcccgg ctcaggctct 20 <210> SEQ ID NO 250 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 250 ccatctcccg gctcaggctc 20 <210> SEQ ID NO 251 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 251 tccatctccc ggctcaggct 20 <210> SEQ ID NO 252 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 252 ctccatctcc cggctcaggc 20 <210> SEQ ID NO 253 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 253 cctccatctc ccggctcagg 20 <210> SEQ ID NO 254 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 254 gcctccatct cccggctcag 20 <210> SEQ ID NO 255 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 255 ggcctccatc tcccggctca 20 <210> SEQ ID NO 256 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 256 tggcctccat ctcccggctc 20 <210> SEQ ID NO 257 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 257 atggcctcca tctcccggct 20 <210> SEQ ID NO 258 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 258 gatggcctcc atctcccggc 20 <210> SEQ ID NO 259 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 259 ggatggcctc catctcccgg 20 <210> SEQ ID NO 260 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 260 cggatggcct ccatctcccg 20 <210> SEQ ID NO 261 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 261 gcggatggcc tccatctccc 20 <210> SEQ ID NO 262 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 262 tgcggatggc ctccatctcc 20 <210> SEQ ID NO 263 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 263 gttccgagcc tctgcctcgc 20 <210> SEQ ID NO 264 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 264 gtcggaggac gaggtcaata 20 <210> SEQ ID NO 265 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 265 agggcctcag cctggccgaa 20 <210> SEQ ID NO 266 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 266 cagggcctca gcctggccga 20 <210> SEQ ID NO 267 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 267 gtcagggcct cagcctggcc 20 <210> SEQ ID NO 268 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 268 cgtcagggcc tcagcctggc 20

<210> SEQ ID NO 269 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 269 agcaaatttc ccgagtaagc 20 <210> SEQ ID NO 270 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 270 aagcaaattt cccgagtaag 20 <210> SEQ ID NO 271 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 271 aaaagcaaat ttcccgagta 20 <210> SEQ ID NO 272 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 272 caaaagcaaa tttcccgagt 20 <210> SEQ ID NO 273 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 273 gcaaaagcaa atttcccgag 20 <210> SEQ ID NO 274 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 274 ggcaaaagca aatttcccga 20 <210> SEQ ID NO 275 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 275 tggcaaaagc aaatttcccg 20 <210> SEQ ID NO 276 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 276 tttggcaaaa gcaaatttcc 20 <210> SEQ ID NO 277 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 277 gtttggcaaa agcaaatttc 20 <210> SEQ ID NO 278 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 278 gggtttggca aaagcaaatt 20 <210> SEQ ID NO 279 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 279 cgggtttggc aaaagcaaat 20 <210> SEQ ID NO 280 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 280 aagcgggttt ggcaaaagca 20 <210> SEQ ID NO 281 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 281 aatatccaaa ccgccgaagc 20 <210> SEQ ID NO 282 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 282 aaatatccaa accgccgaag 20 <210> SEQ ID NO 283 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 283 ataaatatcc aaaccgccga 20 <210> SEQ ID NO 284 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 284 aataaatatc caaaccgccg 20 <210> SEQ ID NO 285 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 285 tcaataaata tccaaaccgc 20 <210> SEQ ID NO 286 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 286 gtcaataaat atccaaaccg 20 <210> SEQ ID NO 287 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 287 ggtcaataaa tatccaaacc 20 <210> SEQ ID NO 288 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 288 aggtcaataa atatccaaac 20 <210> SEQ ID NO 289 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 289 gaggtcaata aatatccaaa 20

<210> SEQ ID NO 290 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 290 acgaggtcaa taaatatcca 20 <210> SEQ ID NO 291 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 291 gacgaggtca ataaatatcc 20 <210> SEQ ID NO 292 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 292 aggacgaggt caataaatat 20 <210> SEQ ID NO 293 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 293 gaggacgagg tcaataaata 20 <210> SEQ ID NO 294 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 294 cggaggacga ggtcaataaa 20 <210> SEQ ID NO 295 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 295 tcggaggacg aggtcaataa 20 <210> SEQ ID NO 296 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 296 agtcggagga cgaggtcaat 20 <210> SEQ ID NO 297 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 297 gagtcggagg acgaggtcaa 20 <210> SEQ ID NO 298 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 298 gcgagtcgga ggacgaggtc 20 <210> SEQ ID NO 299 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 299 agcgagtcgg aggacgaggt 20 <210> SEQ ID NO 300 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 300 cagcgagtcg gaggacgagg 20 <210> SEQ ID NO 301 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 301 tcagcgagtc ggaggacgag 20 <210> SEQ ID NO 302 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 302 gtcagcgagt cggaggacga 20 <210> SEQ ID NO 303 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 303 ctgtcagcga gtcggaggac 20 <210> SEQ ID NO 304 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 304 cctgtcagcg agtcggagga 20 <210> SEQ ID NO 305 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 305 agcctgtcag cgagtcggag 20 <210> SEQ ID NO 306 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 306 gtctcagtgc atccaaaacg 20 <210> SEQ ID NO 307 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 307 ggtctcagtg catccaaaac 20 <210> SEQ ID NO 308 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 308 gggtctcagt gcatccaaaa 20 <210> SEQ ID NO 309 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 309 ggagggcctt ttattcgcga 20 <210> SEQ ID NO 310 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 310

tggagggcct tttattcgcg 20 <210> SEQ ID NO 311 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 311 atggagggcc ttttattcgc 20 <210> SEQ ID NO 312 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 312 gatggagggc cttttattcg 20 <210> SEQ ID NO 313 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 313 agatggaggg ccttttattc 20 <210> SEQ ID NO 314 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 314 cagatggagg gccttttatt 20 <210> SEQ ID NO 315 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 315 gcagatggag ggccttttat 20 <210> SEQ ID NO 316 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 316 ccctcaggct ctctgcttta 20 <210> SEQ ID NO 317 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 317 gccctcaggc tctctgcttt 20 <210> SEQ ID NO 318 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 318 agccctcagg ctctctgctt 20 <210> SEQ ID NO 319 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 319 tagccctcag gctctctgct 20 <210> SEQ ID NO 320 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 320 ttagccctca ggctctctgc 20 <210> SEQ ID NO 321 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 321 tttagccctc aggctctctg 20 <210> SEQ ID NO 322 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 322 atttagccct caggctctct 20 <210> SEQ ID NO 323 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 323 aatttagccc tcaggctctc 20 <210> SEQ ID NO 324 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 324 aaatttagcc ctcaggctct 20 <210> SEQ ID NO 325 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 325 taaatttagc cctcaggctc 20 <210> SEQ ID NO 326 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 326 ttaaatttag ccctcaggct 20 <210> SEQ ID NO 327 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 327 gttaaattta gccctcaggc 20 <210> SEQ ID NO 328 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 328 agttaaattt agccctcagg 20 <210> SEQ ID NO 329 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 329 cagttaaatt tagccctcag 20 <210> SEQ ID NO 330 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 330 acagttaaat ttagccctca 20 <210> SEQ ID NO 331 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 331

gacagttaaa tttagccctc 20 <210> SEQ ID NO 332 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 332 ggacagttaa atttagccct 20 <210> SEQ ID NO 333 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 333 cggacagtta aatttagccc 20 <210> SEQ ID NO 334 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 334 tcggacagtt aaatttagcc 20 <210> SEQ ID NO 335 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 335 ctcggacagt taaatttagc 20 <210> SEQ ID NO 336 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 336 actcggacag ttaaatttag 20 <210> SEQ ID NO 337 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 337 gactcggaca gttaaattta 20 <210> SEQ ID NO 338 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 338 cgactcggac agttaaattt 20 <210> SEQ ID NO 339 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 339 ccgactcgga cagttaaatt 20 <210> SEQ ID NO 340 <211> LENGTH: 20 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 340 tccgactcgg acagttaaat 20 <210> SEQ ID NO 341 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 341 tctcctcacg gaagca 16 <210> SEQ ID NO 342 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 342 ctctcctcac ggaagc 16 <210> SEQ ID NO 343 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 343 cctctcctca cggaag 16 <210> SEQ ID NO 344 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 344 aaacttgctc agcagt 16 <210> SEQ ID NO 345 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 345 caaacttgct cagcag 16 <210> SEQ ID NO 346 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 346 ccaaacttgc tcagca 16 <210> SEQ ID NO 347 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 347 cccaaacttg ctcagc 16 <210> SEQ ID NO 348 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 348 ccccaaactt gctcag 16 <210> SEQ ID NO 349 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 349 tccccaaact tgctca 16 <210> SEQ ID NO 350 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 350 gtttgatgtc cctgtg 16 <210> SEQ ID NO 351 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 351 ggtttgatgt ccctgt 16 <210> SEQ ID NO 352 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide

<400> SEQUENCE: 352 gggtttgatg tccctg 16 <210> SEQ ID NO 353 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 353 acagcctgca ggatct 16 <210> SEQ ID NO 354 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 354 cacagcctgc aggatc 16 <210> SEQ ID NO 355 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 355 ccacagcctg caggat 16 <210> SEQ ID NO 356 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 356 cccacagcct gcagga 16 <210> SEQ ID NO 357 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 357 gcccacagcc tgcagg 16 <210> SEQ ID NO 358 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 358 cgcccacagc ctgcag 16 <210> SEQ ID NO 359 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 359 ccgcccacag cctgca 16 <210> SEQ ID NO 360 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 360 accgcccaca gcctgc 16 <210> SEQ ID NO 361 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 361 caccgcccac agcctg 16 <210> SEQ ID NO 362 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 362 ccaccgccca cagcct 16 <210> SEQ ID NO 363 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 363 cccaccgccc acagcc 16 <210> SEQ ID NO 364 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 364 gcccaccgcc cacagc 16 <210> SEQ ID NO 365 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 365 ccaggcccac cgccca 16 <210> SEQ ID NO 366 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 366 cccaggccca ccgccc 16 <210> SEQ ID NO 367 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 367 tcccaggccc accgcc 16 <210> SEQ ID NO 368 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 368 tgcctgtccc aggccc 16 <210> SEQ ID NO 369 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 369 ctgcctgtcc caggcc 16 <210> SEQ ID NO 370 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 370 gctgcctgtc ccaggc 16 <210> SEQ ID NO 371 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 371 ggtggcacct tcgaaa 16 <210> SEQ ID NO 372 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 372 cggtggcacc ttcgaa 16 <210> SEQ ID NO 373 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide

<400> SEQUENCE: 373 tcggtggcac cttcga 16 <210> SEQ ID NO 374 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 374 agtgagcccg tcctcc 16 <210> SEQ ID NO 375 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 375 cagtgagccc gtcctc 16 <210> SEQ ID NO 376 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 376 gcagtgagcc cgtcct 16 <210> SEQ ID NO 377 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 377 tcccgaatgt ccgaca 16 <210> SEQ ID NO 378 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 378 ttcccgaatg tccgac 16 <210> SEQ ID NO 379 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 379 cttcccgaat gtccga 16 <210> SEQ ID NO 380 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 380 ccttcccgaa tgtccg 16 <210> SEQ ID NO 381 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 381 accttcccga atgtcc 16 <210> SEQ ID NO 382 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 382 caccttcccg aatgtc 16 <210> SEQ ID NO 383 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 383 gcaccttccc gaatgt 16 <210> SEQ ID NO 384 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 384 cgcaccttcc cgaatg 16 <210> SEQ ID NO 385 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 385 atccgctcct gcaact 16 <210> SEQ ID NO 386 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 386 catccgctcc tgcaac 16 <210> SEQ ID NO 387 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 387 ccatccgctc ctgcaa 16 <210> SEQ ID NO 388 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 388 gctccctctg cctgca 16 <210> SEQ ID NO 389 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 389 aggtggatcc gtggcc 16 <210> SEQ ID NO 390 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 390 gggaaggtgg atccgt 16 <210> SEQ ID NO 391 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 391 acaggagcag ggaaag 16 <210> SEQ ID NO 392 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 392 cagactgcgg tgagtt 16 <210> SEQ ID NO 393 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 393 ggctcctggg cggcgc 16 <210> SEQ ID NO 394 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE:

<223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 394 ggcggctcct gggcgg 16 <210> SEQ ID NO 395 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 395 cgcgggcggc tcctgg 16 <210> SEQ ID NO 396 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 396 gagcgcgggc ggctcc 16 <210> SEQ ID NO 397 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 397 ggttcaggga gcgcgg 16 <210> SEQ ID NO 398 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 398 agttctaggg ttcagg 16 <210> SEQ ID NO 399 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 399 cagttctagg gttcag 16 <210> SEQ ID NO 400 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 400 acagttctag ggttca 16 <210> SEQ ID NO 401 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 401 gacagttcta gggttc 16 <210> SEQ ID NO 402 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 402 agacagttct agggtt 16 <210> SEQ ID NO 403 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 403 aagacagttc tagggt 16 <210> SEQ ID NO 404 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 404 gaagacagtt ctaggg 16 <210> SEQ ID NO 405 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 405 cgaagacagt tctagg 16 <210> SEQ ID NO 406 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 406 tcgaagacag ttctag 16 <210> SEQ ID NO 407 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 407 gtcgaagaca gttcta 16 <210> SEQ ID NO 408 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 408 agtcgaagac agttct 16 <210> SEQ ID NO 409 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 409 gagtcgaaga cagttc 16 <210> SEQ ID NO 410 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 410 ggagtcgaag acagtt 16 <210> SEQ ID NO 411 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 411 cggagtcgaa gacagt 16 <210> SEQ ID NO 412 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 412 ccggagtcga agacag 16 <210> SEQ ID NO 413 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 413 cccggagtcg aagaca 16 <210> SEQ ID NO 414 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 414 ccccggagtc gaagac 16 <210> SEQ ID NO 415 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence

<220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 415 gccccggagt cgaaga 16 <210> SEQ ID NO 416 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 416 ggccccggag tcgaag 16 <210> SEQ ID NO 417 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 417 gggccccgga gtcgaa 16 <210> SEQ ID NO 418 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 418 cggttgtgaa ctggca 16 <210> SEQ ID NO 419 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 419 agcggttgtg aactgg 16 <210> SEQ ID NO 420 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 420 gcactttgcg aaccaa 16 <210> SEQ ID NO 421 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 421 tgcactttgc gaacca 16 <210> SEQ ID NO 422 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 422 actttgcgaa ccaacg 16 <210> SEQ ID NO 423 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 423 aaagctttgc actttg 16 <210> SEQ ID NO 424 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 424 tcccgagtaa gcaggc 16 <210> SEQ ID NO 425 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 425 gcagcgcaag tgagga 16 <210> SEQ ID NO 426 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 426 gtcagcgagt cggagg 16 <210> SEQ ID NO 427 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 427 cctgtcagcg agtcgg 16 <210> SEQ ID NO 428 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 428 gcctgtcagc gagtcg 16 <210> SEQ ID NO 429 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 429 agcctgtcag cgagtc 16 <210> SEQ ID NO 430 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 430 gggtctcagt gcatcc 16 <210> SEQ ID NO 431 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 431 aggtttttcc agaggc 16 <210> SEQ ID NO 432 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 432 aaggtttttc cagagg 16 <210> SEQ ID NO 433 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 433 ggtcactgct gggtcc 16 <210> SEQ ID NO 434 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 434 gtggtttctg tctgct 16 <210> SEQ ID NO 435 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 435 cgtggtttct gtctgc 16 <210> SEQ ID NO 436 <211> LENGTH: 16 <212> TYPE: DNA

<213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 436 gaactggcag gcggtg 16 <210> SEQ ID NO 437 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 437 tgtgaactgg caggcg 16 <210> SEQ ID NO 438 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 438 ggttgtgaac tggcag 16 <210> SEQ ID NO 439 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 439 gagcggttgt gaactg 16 <210> SEQ ID NO 440 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 440 actggagctg ggcgga 16 <210> SEQ ID NO 441 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 441 aggactggag ctgggc 16 <210> SEQ ID NO 442 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 442 tcacaggact ggagct 16 <210> SEQ ID NO 443 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 443 atcacaggac tggagc 16 <210> SEQ ID NO 444 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 444 ggatcacagg actgga 16 <210> SEQ ID NO 445 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 445 cagcctggcc gaaaga 16 <210> SEQ ID NO 446 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 446 ctcagcctgg ccgaaa 16 <210> SEQ ID NO 447 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 447 gtcagggcct cagcct 16 <210> SEQ ID NO 448 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 448 cgtcagggcc tcagcc 16 <210> SEQ ID NO 449 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 449 tttgcacttt gcgaac 16 <210> SEQ ID NO 450 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 450 gaaagctttg cacttt 16 <210> SEQ ID NO 451 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 451 aatttcccga gtaagc 16 <210> SEQ ID NO 452 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 452 gcaaatttcc cgagta 16 <210> SEQ ID NO 453 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 453 agcaaatttc ccgagt 16 <210> SEQ ID NO 454 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 454 aagcaaattt cccgag 16 <210> SEQ ID NO 455 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 455 aaagcaaatt tcccga 16 <210> SEQ ID NO 456 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 456 aaaagcaaat ttcccg 16 <210> SEQ ID NO 457 <211> LENGTH: 16

<212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 457 gcaaaagcaa atttcc 16 <210> SEQ ID NO 458 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 458 ggcaaaagca aatttc 16 <210> SEQ ID NO 459 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 459 ttggcaaaag caaatt 16 <210> SEQ ID NO 460 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 460 gtttggcaaa agcaaa 16 <210> SEQ ID NO 461 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 461 ggtttggcaa aagcaa 16 <210> SEQ ID NO 462 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 462 gggtttggca aaagca 16 <210> SEQ ID NO 463 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 463 gcgggtttgg caaaag 16 <210> SEQ ID NO 464 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 464 agcgggtttg gcaaaa 16 <210> SEQ ID NO 465 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 465 aagcgggttt ggcaaa 16 <210> SEQ ID NO 466 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 466 ctccgagagc agcgca 16 <210> SEQ ID NO 467 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 467 gctccgagag cagcgc 16 <210> SEQ ID NO 468 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 468 ggctccgaga gcagcg 16 <210> SEQ ID NO 469 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 469 taaatatcca aaccgc 16 <210> SEQ ID NO 470 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 470 gtcaataaat atccaa 16 <210> SEQ ID NO 471 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 471 aggtcaataa atatcc 16 <210> SEQ ID NO 472 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 472 cgaggtcaat aaatat 16 <210> SEQ ID NO 473 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 473 acgaggtcaa taaata 16 <210> SEQ ID NO 474 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 474 gacgaggtca ataaat 16 <210> SEQ ID NO 475 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 475 ggacgaggtc aataaa 16 <210> SEQ ID NO 476 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 476 aggacgaggt caataa 16 <210> SEQ ID NO 477 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 477 gaggacgagg tcaata 16 <210> SEQ ID NO 478

<211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 478 ggaggacgag gtcaat 16 <210> SEQ ID NO 479 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 479 cggaggacga ggtcaa 16 <210> SEQ ID NO 480 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 480 tcggaggacg aggtca 16 <210> SEQ ID NO 481 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 481 gtcggaggac gaggtc 16 <210> SEQ ID NO 482 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 482 agtcggagga cgaggt 16 <210> SEQ ID NO 483 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 483 gagtcggagg acgagg 16 <210> SEQ ID NO 484 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 484 cgagtcggag gacgag 16 <210> SEQ ID NO 485 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 485 agcgagtcgg aggacg 16 <210> SEQ ID NO 486 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 486 cagcgagtcg gaggac 16 <210> SEQ ID NO 487 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 487 tcagcgagtc ggagga 16 <210> SEQ ID NO 488 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 488 tgtcagcgag tcggag 16 <210> SEQ ID NO 489 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 489 ctgtcagcga gtcgga 16 <210> SEQ ID NO 490 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 490 cagtgcatcc aaaacg 16 <210> SEQ ID NO 491 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 491 tcagtgcatc caaaac 16 <210> SEQ ID NO 492 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 492 ctcagtgcat ccaaaa 16 <210> SEQ ID NO 493 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 493 tctcagtgca tccaaa 16 <210> SEQ ID NO 494 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 494 gtctcagtgc atccaa 16 <210> SEQ ID NO 495 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 495 caataaatac cgagga 16 <210> SEQ ID NO 496 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 496 agacaataaa taccga 16 <210> SEQ ID NO 497 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 497 cagacaataa ataccg 16 <210> SEQ ID NO 498 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 498 cacggaagca cgacac 16

<210> SEQ ID NO 499 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 499 tcacggaagc acgaca 16 <210> SEQ ID NO 500 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 500 ctcacggaag cacgac 16 <210> SEQ ID NO 501 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 501 gtccctctcc tcacgg 16 <210> SEQ ID NO 502 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 502 cgtccctctc ctcacg 16 <210> SEQ ID NO 503 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 503 cccattcacc aacacg 16 <210> SEQ ID NO 504 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 504 ccccattcac caacac 16 <210> SEQ ID NO 505 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 505 tccccattca ccaaca 16 <210> SEQ ID NO 506 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 506 gtccccattc accaac 16 <210> SEQ ID NO 507 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 507 ggtccccatt caccaa 16 <210> SEQ ID NO 508 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 508 cggtccccat tcacca 16 <210> SEQ ID NO 509 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 509 ccggtcccca ttcacc 16 <210> SEQ ID NO 510 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 510 gccggtcccc attcac 16 <210> SEQ ID NO 511 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 511 cgccggtccc cattca 16 <210> SEQ ID NO 512 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 512 ccgccggtcc ccattc 16 <210> SEQ ID NO 513 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 513 accgccggtc cccatt 16 <210> SEQ ID NO 514 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 514 caccgccggt ccccat 16 <210> SEQ ID NO 515 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 515 ccaccgccgg tcccca 16 <210> SEQ ID NO 516 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 516 tccaccgccg gtcccc 16 <210> SEQ ID NO 517 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 517 atccaccgcc ggtccc 16 <210> SEQ ID NO 518 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 518 gatccaccgc cggtcc 16 <210> SEQ ID NO 519 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 519 tgatccaccg ccggtc 16

<210> SEQ ID NO 520 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 520 gtgatccacc gccggt 16 <210> SEQ ID NO 521 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 521 cgtgatccac cgccgg 16 <210> SEQ ID NO 522 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 522 catcctggaa ggcgaa 16 <210> SEQ ID NO 523 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 523 tcatcctgga aggcga 16 <210> SEQ ID NO 524 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 524 agttctcatc ctggaa 16 <210> SEQ ID NO 525 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 525 tagttctcat cctgga 16 <210> SEQ ID NO 526 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 526 gtagttctca tcctgg 16 <210> SEQ ID NO 527 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 527 caggtacagg tagttc 16 <210> SEQ ID NO 528 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 528 ccaggtacag gtagtt 16 <210> SEQ ID NO 529 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 529 gaccaggtac aggtag 16 <210> SEQ ID NO 530 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 530 ctccatgacc aggtac 16 <210> SEQ ID NO 531 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 531 actccatgac caggta 16 <210> SEQ ID NO 532 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 532 tactccatga ccaggt 16 <210> SEQ ID NO 533 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 533 atactccatg accagg 16 <210> SEQ ID NO 534 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 534 aatactccat gaccag 16 <210> SEQ ID NO 535 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 535 taatactcca tgacca 16 <210> SEQ ID NO 536 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 536 gtaatactcc atgacc 16 <210> SEQ ID NO 537 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 537 cgtaatactc catgac 16 <210> SEQ ID NO 538 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 538 cttgctcagc agtgtc 16 <210> SEQ ID NO 539 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 539 acttgctcag cagtgt 16 <210> SEQ ID NO 540 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 540 aacttgctca gcagtg 16

<210> SEQ ID NO 541 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 541 gctccccaaa cttgct 16 <210> SEQ ID NO 542 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 542 cgctccccaa acttgc 16 <210> SEQ ID NO 543 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 543 ccgctcccca aacttg 16 <210> SEQ ID NO 544 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 544 tccgctcccc aaactt 16 <210> SEQ ID NO 545 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 545 atccgctccc caaact 16 <210> SEQ ID NO 546 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 546 aatccgctcc ccaaac 16 <210> SEQ ID NO 547 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 547 gaatccgctc cccaaa 16 <210> SEQ ID NO 548 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 548 ggaatccgct ccccaa 16 <210> SEQ ID NO 549 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 549 cggaatccgc tcccca 16 <210> SEQ ID NO 550 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 550 ccggaatccg ctcccc 16 <210> SEQ ID NO 551 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 551 gccggaatcc gctccc 16 <210> SEQ ID NO 552 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 552 agaagcgcgc catctc 16 <210> SEQ ID NO 553 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 553 tagaagcgcg ccatct 16 <210> SEQ ID NO 554 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 554 gtagaagcgc gccatc 16 <210> SEQ ID NO 555 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 555 ggtagaagcg cgccat 16 <210> SEQ ID NO 556 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 556 aggtagaagc gcgcca 16 <210> SEQ ID NO 557 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 557 caggtagaag cgcgcc 16 <210> SEQ ID NO 558 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 558 ccaggtagaa gcgcgc 16 <210> SEQ ID NO 559 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 559 gccaggtaga agcgcg 16 <210> SEQ ID NO 560 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 560 cgccaggtag aagcgc 16 <210> SEQ ID NO 561 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 561

ccgccaggta gaagcg 16 <210> SEQ ID NO 562 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 562 tccgccaggt agaagc 16 <210> SEQ ID NO 563 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 563 tgacaatctc cgccag 16 <210> SEQ ID NO 564 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 564 atgacaatct ccgcca 16 <210> SEQ ID NO 565 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 565 catgacaatc tccgcc 16 <210> SEQ ID NO 566 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 566 ccatgacaat ctccgc 16 <210> SEQ ID NO 567 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 567 gccatgacaa tctccg 16 <210> SEQ ID NO 568 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 568 ggccatgaca atctcc 16 <210> SEQ ID NO 569 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 569 ccaactgttc tcttag 16 <210> SEQ ID NO 570 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 570 aaccaactgt tctctt 16 <210> SEQ ID NO 571 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 571 ccagtaataa aagctg 16 <210> SEQ ID NO 572 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 572 gtccagtaat aaaagc 16 <210> SEQ ID NO 573 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 573 gttgtccagt aataaa 16 <210> SEQ ID NO 574 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 574 atgttgtcca gtaata 16 <210> SEQ ID NO 575 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 575 taatgttgtc cagtaa 16 <210> SEQ ID NO 576 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 576 ttcaatcctg acccac 16 <210> SEQ ID NO 577 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 577 ggttcaatcc tgaccc 16 <210> SEQ ID NO 578 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 578 tgggttcaat cctgac 16 <210> SEQ ID NO 579 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 579 gatgggttca atcctg 16 <210> SEQ ID NO 580 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 580 aggatgggtt caatcc 16 <210> SEQ ID NO 581 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 581 agaggatggg ttcaat 16 <210> SEQ ID NO 582 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 582

atagaggatg ggttca 16 <210> SEQ ID NO 583 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 583 ccctcctgtg ggaaca 16 <210> SEQ ID NO 584 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 584 gtccctcctg tgggaa 16 <210> SEQ ID NO 585 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 585 cagtccctcc tgtggg 16 <210> SEQ ID NO 586 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 586 agcagtccct cctgtg 16 <210> SEQ ID NO 587 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 587 actcagctgt gggaag 16 <210> SEQ ID NO 588 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 588 cccactcagc tgtggg 16 <210> SEQ ID NO 589 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 589 accccactca gctgtg 16 <210> SEQ ID NO 590 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 590 acaccccact cagctg 16 <210> SEQ ID NO 591 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 591 gcacacccca ctcagc 16 <210> SEQ ID NO 592 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 592 tcagcacacc ccactc 16 <210> SEQ ID NO 593 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 593 gtggtcctaa gactgg 16 <210> SEQ ID NO 594 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 594 gatgtggtcc taagac 16 <210> SEQ ID NO 595 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 595 cagatgtggt cctaag 16 <210> SEQ ID NO 596 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 596 cctccacaga tgtggt 16 <210> SEQ ID NO 597 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 597 cacctccaca gatgtg 16 <210> SEQ ID NO 598 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 598 ggccacctcc acagat 16 <210> SEQ ID NO 599 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 599 tgcttggctc tggcca 16 <210> SEQ ID NO 600 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 600 actgcttggc tctggc 16 <210> SEQ ID NO 601 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 601 agactgcttg gctctg 16 <210> SEQ ID NO 602 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 602 ggagactgct tggctc 16 <210> SEQ ID NO 603 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide

<400> SEQUENCE: 603 tgcagacccc tcttct 16 <210> SEQ ID NO 604 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 604 ctcctccctt gacatg 16 <210> SEQ ID NO 605 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 605 ccagaccccc atgttc 16 <210> SEQ ID NO 606 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 606 gtccagaccc ccatgt 16 <210> SEQ ID NO 607 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 607 gggtccagac ccccat 16 <210> SEQ ID NO 608 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 608 accttctgca gggact 16 <210> SEQ ID NO 609 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 609 taaaccttct gcaggg 16 <210> SEQ ID NO 610 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 610 gaaaagccct gcccct 16 <210> SEQ ID NO 611 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 611 taggaaaagc cctgcc 16 <210> SEQ ID NO 612 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 612 cttaggaaaa gccctg 16 <210> SEQ ID NO 613 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 613 tgcttaggaa aagccc 16 <210> SEQ ID NO 614 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 614 tctgcttagg aaaagc 16 <210> SEQ ID NO 615 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 615 ctcctctgct taggaa 16 <210> SEQ ID NO 616 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 616 ccctcctctg cttagg 16 <210> SEQ ID NO 617 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 617 ctgatttgag gaaggg 16 <210> SEQ ID NO 618 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 618 tcctgatttg aggaag 16 <210> SEQ ID NO 619 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 619 cctcctgatt tgagga 16 <210> SEQ ID NO 620 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 620 gacctcctga tttgag 16 <210> SEQ ID NO 621 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 621 aagacctcct gatttg 16 <210> SEQ ID NO 622 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 622 ccaagacctc ctgatt 16 <210> SEQ ID NO 623 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 623 ctgcttccaa gacctc 16 <210> SEQ ID NO 624 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide

<400> SEQUENCE: 624 agctgcttcc aagacc 16 <210> SEQ ID NO 625 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 625 gcagctgctt ccaaga 16 <210> SEQ ID NO 626 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 626 ctggtggaga accaga 16 <210> SEQ ID NO 627 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 627 ctctggtgga gaacca 16 <210> SEQ ID NO 628 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 628 ttctctggtg gagaac 16 <210> SEQ ID NO 629 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 629 gattctctgg tggaga 16 <210> SEQ ID NO 630 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 630 acttactgtt tcatcc 16 <210> SEQ ID NO 631 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 631 cggaccccct cccctc 16 <210> SEQ ID NO 632 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 632 gacggacccc ctcccc 16 <210> SEQ ID NO 633 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 633 ctgacggacc ccctcc 16 <210> SEQ ID NO 634 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 634 ccctgacgga ccccct 16 <210> SEQ ID NO 635 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 635 aagccctcac cttttc 16 <210> SEQ ID NO 636 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 636 ggaagccctc accttt 16 <210> SEQ ID NO 637 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 637 cgggaagccc tcacct 16 <210> SEQ ID NO 638 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 638 cccgggaagc cctcac 16 <210> SEQ ID NO 639 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 639 cacccgggaa gccctc 16 <210> SEQ ID NO 640 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 640 gccacccggg aagccc 16 <210> SEQ ID NO 641 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 641 acgccacccg ggaagc 16 <210> SEQ ID NO 642 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 642 ctgttcagga agtccc 16 <210> SEQ ID NO 643 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 643 ttctgttcag gaagtc 16 <210> SEQ ID NO 644 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 644 gcttctgttc aggaag 16 <210> SEQ ID NO 645 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE:

<223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 645 gcttgggccc acccct 16 <210> SEQ ID NO 646 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 646 aggcttgggc ccaccc 16 <210> SEQ ID NO 647 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 647 cgaggcttgg gcccac 16 <210> SEQ ID NO 648 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 648 agcgaggctt gggccc 16 <210> SEQ ID NO 649 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 649 agagcgaggc ttgggc 16 <210> SEQ ID NO 650 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 650 gcagagcgag gcttgg 16 <210> SEQ ID NO 651 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 651 gagcagagcg aggctt 16 <210> SEQ ID NO 652 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 652 aaaggagcag agcgag 16 <210> SEQ ID NO 653 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 653 caaaaggagc agagcg 16 <210> SEQ ID NO 654 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 654 tggaccaaaa ggagca 16 <210> SEQ ID NO 655 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 655 cctggaccaa aaggag 16 <210> SEQ ID NO 656 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 656 cacctggacc aaaagg 16 <210> SEQ ID NO 657 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 657 cgcacctgga ccaaaa 16 <210> SEQ ID NO 658 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 658 gaccgcacct ggacca 16 <210> SEQ ID NO 659 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 659 accttgtagt ggacga 16 <210> SEQ ID NO 660 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 660 tcaccttgta gtggac 16 <210> SEQ ID NO 661 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 661 gctcaccttg tagtgg 16 <210> SEQ ID NO 662 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 662 ggagaggagg cgatag 16 <210> SEQ ID NO 663 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 663 agggagagga ggcgat 16 <210> SEQ ID NO 664 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 664 ctcctgctca gaggga 16 <210> SEQ ID NO 665 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 665 gtgctcctgc tcagag 16 <210> SEQ ID NO 666 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence

<220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 666 aggtgctcct gctcag 16 <210> SEQ ID NO 667 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 667 agaggtgctc ctgctc 16 <210> SEQ ID NO 668 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 668 agagaggtgc tcctgc 16 <210> SEQ ID NO 669 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 669 accccgcccc cgctca 16 <210> SEQ ID NO 670 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 670 ctaccccgcc cccgct 16 <210> SEQ ID NO 671 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 671 acctaccccg cccccg 16 <210> SEQ ID NO 672 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 672 gtacctaccc cgcccc 16 <210> SEQ ID NO 673 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 673 aggtacctac cccgcc 16 <210> SEQ ID NO 674 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 674 gggaggttcc cgcagc 16 <210> SEQ ID NO 675 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 675 gtccttactc caactt 16 <210> SEQ ID NO 676 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 676 ctgtccttac tccaac 16 <210> SEQ ID NO 677 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 677 cactgtcctt actcca 16 <210> SEQ ID NO 678 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 678 ggcactgtcc ttactc 16 <210> SEQ ID NO 679 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 679 taggcactgt ccttac 16 <210> SEQ ID NO 680 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 680 ggtaggcact gtcctt 16 <210> SEQ ID NO 681 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 681 gtcactgctg ggtcct 16 <210> SEQ ID NO 682 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 682 aggtcactgc tgggtc 16 <210> SEQ ID NO 683 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 683 ctaggtcact gctggg 16 <210> SEQ ID NO 684 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 684 gtctaggtca ctgctg 16 <210> SEQ ID NO 685 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 685 aagtctaggt cactgc 16 <210> SEQ ID NO 686 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 686 gcactccatt gtctca 16 <210> SEQ ID NO 687 <211> LENGTH: 16 <212> TYPE: DNA

<213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 687 ctgcactcca ttgtct 16 <210> SEQ ID NO 688 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 688 ccctgcactc cattgt 16 <210> SEQ ID NO 689 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 689 ccccctgcac tccatt 16 <210> SEQ ID NO 690 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 690 cttgctgagt caggag 16 <210> SEQ ID NO 691 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 691 tccttgctga gtcagg 16 <210> SEQ ID NO 692 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 692 cttccttgct gagtca 16 <210> SEQ ID NO 693 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 693 accttccttg ctgagt 16 <210> SEQ ID NO 694 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 694 ggaccttcct tgctga 16 <210> SEQ ID NO 695 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 695 caggaccttc cttgct 16 <210> SEQ ID NO 696 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 696 agccctccag gacctt 16 <210> SEQ ID NO 697 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 697 tagctcccca ctccag 16 <210> SEQ ID NO 698 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 698 gatagctccc cactcc 16 <210> SEQ ID NO 699 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 699 cagatagctc cccact 16 <210> SEQ ID NO 700 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 700 ctcagatagc tcccca 16 <210> SEQ ID NO 701 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 701 agctcagata gctccc 16 <210> SEQ ID NO 702 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 702 tcagctcaga tagctc 16 <210> SEQ ID NO 703 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 703 tctcagctca gatagc 16 <210> SEQ ID NO 704 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 704 gagtcctctc ctgctt 16 <210> SEQ ID NO 705 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 705 ggaggagtcc tctcct 16 <210> SEQ ID NO 706 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 706 gaggaggagt cctctc 16 <210> SEQ ID NO 707 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 707 caaaagggca cccaga 16 <210> SEQ ID NO 708 <211> LENGTH: 16

<212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 708 agcaaaaggg caccca 16 <210> SEQ ID NO 709 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 709 ggatccccag tattgt 16 <210> SEQ ID NO 710 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 710 ctggatcccc agtatt 16 <210> SEQ ID NO 711 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 711 tgctggatcc ccagta 16 <210> SEQ ID NO 712 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 712 attctctaga ctgcaa 16 <210> SEQ ID NO 713 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 713 taattctcta gactgc 16 <210> SEQ ID NO 714 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 714 tctaattctc tagact 16 <210> SEQ ID NO 715 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 715 tctctaattc tctaga 16 <210> SEQ ID NO 716 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 716 ctccataatt ctctaa 16 <210> SEQ ID NO 717 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 717 actctccata attctc 16 <210> SEQ ID NO 718 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 718 acactctcca taattc 16 <210> SEQ ID NO 719 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 719 ccacactctc cataat 16 <210> SEQ ID NO 720 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 720 tgccacactc tccata 16 <210> SEQ ID NO 721 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 721 cccatgccca tcctgc 16 <210> SEQ ID NO 722 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 722 ggacagagaa atgttg 16 <210> SEQ ID NO 723 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 723 ggcataggac agagaa 16 <210> SEQ ID NO 724 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 724 gtggcatagg acagag 16 <210> SEQ ID NO 725 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 725 tggtggcata ggacag 16 <210> SEQ ID NO 726 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 726 cttactctgc ccctcc 16 <210> SEQ ID NO 727 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 727 accttactct gcccct 16 <210> SEQ ID NO 728 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 728 tgaccttact ctgccc 16 <210> SEQ ID NO 729

<211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 729 gctgacctta ctctgc 16 <210> SEQ ID NO 730 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 730 ctgctgacct tactct 16 <210> SEQ ID NO 731 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 731 ctctgctgac cttact 16 <210> SEQ ID NO 732 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 732 gcctctgctg acctta 16 <210> SEQ ID NO 733 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 733 ccatggctct gagtca 16 <210> SEQ ID NO 734 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 734 agccatggct ctgagt 16 <210> SEQ ID NO 735 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 735 taagccatgg ctctga 16 <210> SEQ ID NO 736 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 736 tagcctgctg tgactc 16 <210> SEQ ID NO 737 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 737 atgggaggct gttggc 16 <210> SEQ ID NO 738 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 738 ccatgggagg ctgttg 16 <210> SEQ ID NO 739 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 739 ggccatggga ggctgt 16 <210> SEQ ID NO 740 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 740 gtgcagagag gccatg 16 <210> SEQ ID NO 741 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 741 gagctcccag catgac 16 <210> SEQ ID NO 742 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 742 agggagctcc cagcat 16 <210> SEQ ID NO 743 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 743 gccatagagc ccactt 16 <210> SEQ ID NO 744 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 744 gggccataga gcccac 16 <210> SEQ ID NO 745 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 745 atgctggccc tcctgg 16 <210> SEQ ID NO 746 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 746 agctgcccca tgctgg 16 <210> SEQ ID NO 747 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 747 cgcccctggc agctgc 16 <210> SEQ ID NO 748 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 748 tgcgcccctg gcagct 16 <210> SEQ ID NO 749 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 749 gctgcgcccc tggcag 16

<210> SEQ ID NO 750 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 750 cggctgcgcc cctggc 16 <210> SEQ ID NO 751 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 751 gtcggctgcg cccctg 16 <210> SEQ ID NO 752 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 752 ctgtcggctg cgcccc 16 <210> SEQ ID NO 753 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 753 gcctgtcggc tgcgcc 16 <210> SEQ ID NO 754 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 754 ctgcctgtcg gctgcg 16 <210> SEQ ID NO 755 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 755 acctgcctgt cggctg 16 <210> SEQ ID NO 756 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 756 acacctgcct gtcggc 16 <210> SEQ ID NO 757 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 757 gaacacctgc ctgtcg 16 <210> SEQ ID NO 758 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 758 ccgaacacct gcctgt 16 <210> SEQ ID NO 759 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 759 cgccgaacac ctgcct 16 <210> SEQ ID NO 760 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 760 cctgggcacc tgttgg 16 <210> SEQ ID NO 761 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 761 gtgcctgggc acctgt 16 <210> SEQ ID NO 762 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 762 cgccctccca gtgcct 16 <210> SEQ ID NO 763 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 763 accgccctcc cagtgc 16 <210> SEQ ID NO 764 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 764 tcaccgccct cccagt 16 <210> SEQ ID NO 765 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 765 agtcaccgcc ctccca 16 <210> SEQ ID NO 766 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 766 tgagtcaccg ccctcc 16 <210> SEQ ID NO 767 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 767 cgtgagtcac cgccct 16 <210> SEQ ID NO 768 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 768 caaagctggt tctccc 16 <210> SEQ ID NO 769 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 769 tgcaaagctg gttctc 16 <210> SEQ ID NO 770 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 770 tctgcaaagc tggttc 16

<210> SEQ ID NO 771 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 771 tgtctgcaaa gctggt 16 <210> SEQ ID NO 772 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 772 cctgtctgca aagctg 16 <210> SEQ ID NO 773 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 773 cgcctgtctg caaagc 16 <210> SEQ ID NO 774 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 774 ttgtccctcc tggatc 16 <210> SEQ ID NO 775 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 775 agttgtccct cctgga 16 <210> SEQ ID NO 776 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 776 aaagttgtcc ctcctg 16 <210> SEQ ID NO 777 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 777 ccaaagttgt ccctcc 16 <210> SEQ ID NO 778 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 778 acccaaagtt gtccct 16 <210> SEQ ID NO 779 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 779 gaacccaaag ttgtcc 16 <210> SEQ ID NO 780 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 780 gaagaaccca aagttg 16 <210> SEQ ID NO 781 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 781 ccagaagaac ccaaag 16 <210> SEQ ID NO 782 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 782 cacccagaag aaccca 16 <210> SEQ ID NO 783 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 783 gcagaaccta caaaag 16 <210> SEQ ID NO 784 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 784 gtgcagaacc tacaaa 16 <210> SEQ ID NO 785 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 785 gggtgcagaa cctaca 16 <210> SEQ ID NO 786 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 786 gtgggtgcag aaccta 16 <210> SEQ ID NO 787 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 787 ccacacggct catagg 16 <210> SEQ ID NO 788 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 788 acccacacgg ctcata 16 <210> SEQ ID NO 789 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 789 tgacccacac ggctca 16 <210> SEQ ID NO 790 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 790 gctgacccac acggct 16 <210> SEQ ID NO 791 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 791 tggctgaccc acacgg 16

<210> SEQ ID NO 792 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 792 ggtggctgac ccacac 16 <210> SEQ ID NO 793 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 793 atggtggctg acccac 16 <210> SEQ ID NO 794 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 794 gaatggtggc tgaccc 16 <210> SEQ ID NO 795 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 795 ctaaaggacg caggga 16 <210> SEQ ID NO 796 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 796 aactaaagga cgcagg 16 <210> SEQ ID NO 797 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 797 gagaactaaa ggacgc 16 <210> SEQ ID NO 798 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 798 attggtccca agcccc 16 <210> SEQ ID NO 799 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 799 ccattggtcc caagcc 16 <210> SEQ ID NO 800 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 800 gcccattggt cccaag 16 <210> SEQ ID NO 801 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 801 acgcccattg gtccca 16 <210> SEQ ID NO 802 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 802 ccacgcccat tggtcc 16 <210> SEQ ID NO 803 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 803 caccacgccc attggt 16 <210> SEQ ID NO 804 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 804 agacccaact ccaccc 16 <210> SEQ ID NO 805 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 805 tcacctcgcc cctctt 16 <210> SEQ ID NO 806 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 806 cctcacctcg cccctc 16 <210> SEQ ID NO 807 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 807 agcccctcac ctcgcc 16 <210> SEQ ID NO 808 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 808 ctcaaagccc cccacg 16 <210> SEQ ID NO 809 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 809 atcctcaaag cccccc 16 <210> SEQ ID NO 810 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 810 ggatcctcaa agcccc 16 <210> SEQ ID NO 811 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 811 gcggatcctc aaagcc 16 <210> SEQ ID NO 812 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 812

gcgcggatcc tcaaag 16 <210> SEQ ID NO 813 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 813 gggcgcggat cctcaa 16 <210> SEQ ID NO 814 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 814 gagctgcagc cggaga 16 <210> SEQ ID NO 815 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 815 aggagctgca gccgga 16 <210> SEQ ID NO 816 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 816 cggaggagct gcagcc 16 <210> SEQ ID NO 817 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 817 acccggagga gctgca 16 <210> SEQ ID NO 818 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 818 gcacccggag gagctg 16 <210> SEQ ID NO 819 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 819 gggcacccgg aggagc 16 <210> SEQ ID NO 820 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 820 cagggcaccc ggagga 16 <210> SEQ ID NO 821 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 821 tgcagggcac ccggag 16 <210> SEQ ID NO 822 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 822 cctgcagggc acccgg 16 <210> SEQ ID NO 823 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 823 cgacacctgc agggca 16 <210> SEQ ID NO 824 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 824 cacgacacct gcaggg 16 <210> SEQ ID NO 825 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 825 agcacgacac ctgcag 16 <210> SEQ ID NO 826 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 826 gaagcacgac acctgc 16 <210> SEQ ID NO 827 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 827 ccaggtagtt ctcatc 16 <210> SEQ ID NO 828 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 828 caccaggtag ttctca 16 <210> SEQ ID NO 829 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 829 ctcaccaggt agttct 16 <210> SEQ ID NO 830 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 830 agctcaccag gtagtt 16 <210> SEQ ID NO 831 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 831 ggagctcacc aggtag 16 <210> SEQ ID NO 832 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 832 ccggagctca ccaggt 16 <210> SEQ ID NO 833 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 833

gcccggagct caccag 16 <210> SEQ ID NO 834 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 834 tagagcttcc tctccc 16 <210> SEQ ID NO 835 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 835 cctagagctt cctctc 16 <210> SEQ ID NO 836 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 836 atcctagagc ttcctc 16 <210> SEQ ID NO 837 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 837 caatcctaga gcttcc 16 <210> SEQ ID NO 838 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 838 cccaatccta gagctt 16 <210> SEQ ID NO 839 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 839 cccccaatcc tagagc 16 <210> SEQ ID NO 840 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 840 cacccccaat cctaga 16 <210> SEQ ID NO 841 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 841 agcaccccca atccta 16 <210> SEQ ID NO 842 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 842 gcagcacccc caatcc 16 <210> SEQ ID NO 843 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 843 gggcagcacc cccaat 16 <210> SEQ ID NO 844 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 844 tgacacaccc tcttac 16 <210> SEQ ID NO 845 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 845 cctgacacac cctctt 16 <210> SEQ ID NO 846 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 846 cacctgacac accctc 16 <210> SEQ ID NO 847 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 847 tccacctgac acaccc 16 <210> SEQ ID NO 848 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 848 catccacctg acacac 16 <210> SEQ ID NO 849 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 849 ctcatccacc tgacac 16 <210> SEQ ID NO 850 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 850 ccctcatcca cctgac 16 <210> SEQ ID NO 851 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 851 gcccctcatc cacctg 16 <210> SEQ ID NO 852 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 852 aggcccctca tccacc 16 <210> SEQ ID NO 853 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 853 gaaggcccct catcca 16 <210> SEQ ID NO 854 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide

<400> SEQUENCE: 854 aggtaagaga cccccc 16 <210> SEQ ID NO 855 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 855 ccaggtaaga gacccc 16 <210> SEQ ID NO 856 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 856 ttccaggtaa gagacc 16 <210> SEQ ID NO 857 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 857 ccattccagg taagag 16 <210> SEQ ID NO 858 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 858 tcccattcca ggtaag 16 <210> SEQ ID NO 859 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 859 tatcccattc caggta 16 <210> SEQ ID NO 860 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 860 cctatcccat tccagg 16 <210> SEQ ID NO 861 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 861 gacctatccc attcca 16 <210> SEQ ID NO 862 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 862 aagacctatc ccattc 16 <210> SEQ ID NO 863 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 863 tgaagaccta tcccat 16 <210> SEQ ID NO 864 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 864 tggccccgtt agaatt 16 <210> SEQ ID NO 865 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 865 agtggccccg ttagaa 16 <210> SEQ ID NO 866 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 866 gcagtggccc cgttag 16 <210> SEQ ID NO 867 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 867 aggcagtggc cccgtt 16 <210> SEQ ID NO 868 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 868 ctaggcagtg gccccg 16 <210> SEQ ID NO 869 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 869 ccctaggcag tggccc 16 <210> SEQ ID NO 870 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 870 aggtcccaga cactcc 16 <210> SEQ ID NO 871 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 871 ataggtccca gacact 16 <210> SEQ ID NO 872 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 872 gaataggtcc cagaca 16 <210> SEQ ID NO 873 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 873 gagaataggt cccaga 16 <210> SEQ ID NO 874 <211> LENGTH: 16 <212> TYPE: DNA <213> ORGANISM: Artificial sequence <220> FEATURE: <223> OTHER INFORMATION: Synthetic oligonucleotide <400> SEQUENCE: 874 cagagaatag gtccca 16

Claims

1-167. (canceled)

168. A compound comprising a modified oligonucleotide consisting of 10-30 linked nucleosides and having a nucleobase sequence comprising a portion of at least 8 contiguous nucleobases 100% complementary to an equal length portion of nucleobases 730-748, 1317-1366, 1343-1368, or 2748-2791 of SEQ ID NO: 1 or nucleobases 8603-8619, 10201-10216, 10202-10218, or 13836-13851 of SEQ ID NO: 2, wherein the nucleobase sequence of the modified oligonucleotide is at least 90% complementary to SEQ ID NO: 1 or SEQ ID NO: 2 as measured over the entirety of the modified oligonucleotide.

169. The compound of claim 168, wherein the modified oligonucleotide is a single-stranded oligonucleotide.

170. The compound of claim 169, wherein at least one nucleoside comprises a modified nucleobase.

171. The compound of claim 170, wherein the modified nucleobase is a 5-methylcytosine.

172. The compound of claim 171, wherein at least one internucleoside linkage is a modified internucleoside linkage.

173. The compound of claim 170, wherein each internucleoside linkage is a phosphorothioate internucleoside linkage.

174. The compound of claim 173, wherein at least one nucleoside comprises a modified sugar.

175. The compound of claim 173, wherein at least two nucleosides comprise a modified sugar.

176. The compound of claim 175, wherein each of the modified sugars have the same modification.

177. The compound of claim 175, wherein at least one of the modified sugars has a different modification.

178. The compound of claim 174, wherein the modified sugar is a bicyclic sugar.

179. The compound of claim 178, wherein the bicyclic sugar is selected from among cEt, LNA, .alpha.-L-LNA, ENA, and 2'-thio LNA.

180. The compound of claim 174, wherein the at least one nucleoside comprising a modified sugar is a 2'-substituted nucleoside.

181. The compound of claim 180, wherein the 2'-substituted nucleoside is selected from among: 2'-OCH.sub.3, 2'-F, and 2'-O-methoxyethyl.

182. The compound of claim 169, wherein the modified oligonucleotide comprises: a. a gap segment consisting of linked deoxynucleosides; b. a 5' wing segment consisting of liked nucleosides; c. a 3' wing segment consisting of linked nucleosides; d. wherein the gap segment is positioned between the 5' wing segment and the 3' wing segment and wherein each nucleoside of each wing segment comprises a modified sugar.

183. The compound of claim 182, wherein the modified oligonucleotide consists of 16, 17, 18, 19, or 20 linked nucleosides.

184. A compound comprising a modified oligonucleotide consisting of 10-30 linked nucleosides and having a nucleobase sequence comprising a portion of at least 8 contiguous nucleobases of any of SEQ ID NOs: 23, 25, 26, 28, 29, 30, 31, and 32.

185. The compound of claim 184, wherein the modified oligonucleotide is a single-stranded oligonucleotide.

186. The compound of claim 185, wherein at least one nucleoside comprises a modified nucleobase.

187. The compound of claim 186, wherein the modified nucleobase is a 5-methylcytosine.

188. The compound of claim 187, wherein at least one internucleoside linkage is a modified internucleoside linkage.

189. The compound of claim 188, wherein each internucleoside linkage is a phosphorothioate internucleoside linkage.

190. The compound of claim 189, wherein at least one nucleoside comprises a modified sugar.

191. The compound of claim 189, wherein at least two nucleosides comprise a modified sugar.

192. The compound of claim 191, wherein each of the modified sugars have the same modification.

193. The compound of claim 191, wherein at least one of the modified sugars has a different modification.

194. The compound of claim 190, wherein the modified sugar is a bicyclic sugar.

195. The compound of claim 194, wherein the bicyclic sugar is selected from among cEt, LNA, .alpha.-L-LNA, ENA, and 2'-thio LNA.

196. The compound of claim 194, wherein the at least one nucleoside comprising a modified sugar is a 2'-substituted nucleoside.

197. The compound of claim 196, wherein the 2'-substituted nucleoside is selected from among: 2'-OCH.sub.3, 2'-F, and 2'-O-methoxyethyl.

198. The compound of claim 185, wherein the modified oligonucleotide comprises: a. a gap segment consisting of linked deoxynucleosides; b. a 5' wing segment consisting of liked nucleosides; c. a 3' wing segment consisting of linked nucleosides; d. wherein the gap segment is positioned between the 5' wing segment and the 3' wing segment and wherein each nucleoside of each wing segment comprises a modified sugar.

199. The compound of claim 198, wherein the modified oligonucleotide consists of 16, 17, 18, 19, or 20 linked nucleosides.

200. A compound comprising a modified oligonucleotide having a nucleobase sequence as set forth in SEQ ID NO: 30, wherein the modified oligonucleotide consists of 16 linked nucleosides and comprises: a. a gap segment consisting of eight linked deoxynucleosides; b. a 5' wing segment consisting of four linked nucleosides and having a E-E-K-K 5'-wing motif; c. a 3' wing segment consisting of four linked nucleosides and having a K-K-E-E 3'-wing motif; d. wherein the gap segment is positioned between the 5' wing segment and the 3' wing segment; e. wherein each E represents 2'-O-methoxyethyl sugar and each K represents a cEt sugar; f. wherein each internucleoside linkage is a phosphorothioate internucleoside linkage; and g. wherein each cytosine residue is a 5-methyl cytosine.

201. A composition comprising the compound of claim 168 or a salt thereof and a pharmaceutically acceptable carrier or diluent.

202. A composition comprising the compound of claim 184 or a salt thereof and a pharmaceutically acceptable carrier or diluent.

203. A composition comprising the compound of claim 200 or a salt thereof and a pharmaceutically acceptable carrier or diluent.

204. A method of treating DM1 in an animal comprising administering to an animal in need thereof a composition according to claim 201.

205. A method of treating DM1 in an animal comprising administering to an animal in need thereof a composition according to claim 202.

206. A method of treating DM1 in an animal comprising administering to an animal in need thereof a composition according to claim 203.