U.S. patent application number 11/776043 was filed with the patent office on 2013-08-08 for modified clostridial toxins with enhanced translocation capability and enhanced targeting activity.
The applicant listed for this patent is Kei Roger Aoki, Ester G. Fernandez-Salas, Joseph Francis, Marcella A. Gilmore, Shengwen Li, Lance E. Steward. Invention is credited to Kei Roger Aoki, Ester G. Fernandez-Salas, Joseph Francis, Marcella A. Gilmore, Shengwen Li, Lance E. Steward.
Application Number | 20130203148 11/776043 |
Document ID | / |
Family ID | 39721743 |
Filed Date | 2013-08-08 |
United States Patent
Application |
20130203148 |
Kind Code |
A1 |
Steward; Lance E. ; et
al. |
August 8, 2013 |
MODIFIED CLOSTRIDIAL TOXINS WITH ENHANCED TRANSLOCATION CAPABILITY
AND ENHANCED TARGETING ACTIVITY
Abstract
The specification discloses modified Clostridial toxins
comprising a Clostridial toxin enzymatic domain, a Clostridial
toxin translocation domain, a translocation facilitating domain and
an enhanced targeting domain; polynucleotide molecules encoding
such modified Clostridial toxins; and method of producing such
modified Clostridial toxins.
Inventors: |
Steward; Lance E.; (Irvine,
CA) ; Francis; Joseph; (Aliso Viejo, CA) ;
Fernandez-Salas; Ester G.; (Fullerton, CA) ; Gilmore;
Marcella A.; (Santa Ana, CA) ; Li; Shengwen;
(Irvine, CA) ; Aoki; Kei Roger; (Coto de Caza,
CA) |
|
Applicant: |
Name |
City |
State |
Country |
Type |
Steward; Lance E.
Francis; Joseph
Fernandez-Salas; Ester G.
Gilmore; Marcella A.
Li; Shengwen
Aoki; Kei Roger |
Irvine
Aliso Viejo
Fullerton
Santa Ana
Irvine
Coto de Caza |
CA
CA
CA
CA
CA
CA |
US
US
US
US
US
US |
|
|
Family ID: |
39721743 |
Appl. No.: |
11/776043 |
Filed: |
July 11, 2007 |
Related U.S. Patent Documents
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Application
Number |
Filing Date |
Patent Number |
|
|
60807063 |
Jul 11, 2006 |
|
|
|
Current U.S.
Class: |
435/188 |
Current CPC
Class: |
C07K 2319/01 20130101;
A61P 21/00 20180101; C12N 9/52 20130101; A61P 27/16 20180101; A61P
9/00 20180101; C12N 9/96 20130101; A61P 25/00 20180101; C07K 14/33
20130101; A61P 27/02 20180101; A61K 38/00 20130101; A61P 35/00
20180101; A61P 25/04 20180101 |
Class at
Publication: |
435/188 |
International
Class: |
C12N 9/96 20060101
C12N009/96 |
Claims
1. A modified Clostridial botulinum toxin comprising: a) a
Clostridial botulinum toxin type A enzymatic domain; b) a
Clostridial botulinum toxin type A translocation domain; c) a
translocation facilitating domain comprising an enveloped virus
fusogenic peptide domain capable of facilitating a translocation
step of a Clostridial toxin intoxication process; and, d) an
enhanced targeting domain consisting essentially of a modified
Clostridial botulinum H.sub.CC targeting domain capable of
executing a cell binding step of a Clostridial botulinum toxin
intoxication process; wherein the modified Clostridial botulinum
toxin comprises a linear amino-to-carboxyl order of the Clostridial
botulinum toxin enzymatic domain, the Clostridial botulinum toxin
translocation domain, the translocation facilitating domain and the
enhanced targeting domain, and wherein the modified Clostridial
botulinum H.sub.CC targeting domain comprises a Clostridial
botulinum serotype A NTNH .beta.-fold and exhibits enhanced binding
activity for an endogenous Clostridial botulinum toxin receptor
relative to the binding activity of a naturally-occurring
Clostridial botulinum serotype A H.sub.CC targeting domain.
2.-27. (canceled)
Description
[0001] This Non-Provisional patent application claims priority
pursuant to 35 U.S.C. .sctn.119(e) to U.S. Provisional Patent
Application Ser. No. 60/807,063 filed Jul. 11, 2006, which is
hereby incorporated by reference in its entirety.
[0002] All patents and publications cited in this application are
hereby incorporated by reference in their entirety.
[0003] The ability of Clostridial toxins, such as, e.g., Botulinum
neurotoxins (BoNTs), BoNT/A, BoNT/B, BoNT/C1, BoNT/D, BoNT/E,
BoNT/F and BoNT/G, and Tetanus neurotoxin (TeNT), to inhibit
neuronal transmission are being exploited in a wide variety of
therapeutic and cosmetic applications, see e.g., William J. Lipham,
COSMETIC AND CLINICAL APPLICATIONS OF BOTULINUM TOXIN (Slack, Inc.,
2004). Clostridial toxins commercially available as pharmaceutical
compositions include, BoNT/A preparations, such as, e.g.,
BOTOX.RTM. (Allergan, Inc., Irvine, Calif.),
Dysport.RTM./Reloxin.RTM., (Beaufour Ipsen, Porton Down, England),
Linurase.RTM. (Prollenium, Inc., Ontario, Canada), Neuronox.RTM.
(Medy-Tox, Inc., Ochang-myeon, South Korea) BTX-A (Lanzhou
Institute Biological Products, China) and Xeomin.RTM. (Merz
Pharmaceuticals, GmbH., Frankfurt, Germany); and BoNT/B
preparations, such as, e.g., MyoBloc.TM./NeuroBloc.TM. (Elan
Pharmaceuticals, San Francisco, Calif.). As an example, BOTOX.RTM.
is currently approved in one or more countries for the following
indications: achalasia, adult spasticity, anal fissure, back pain,
blepharospasm, bruxism, cervical dystonia, essential tremor,
glabellar lines or hyperkinetic facial lines, headache, hemifacial
spasm, hyperactivity of bladder, hyperhidrosis, juvenile cerebral
palsy, multiple sclerosis, myoclonic disorders, nasal labial lines,
spasmodic dysphonia, strabismus and VII nerve disorder.
[0004] Clostridial toxin therapies are successfully used for many
indications. Generally, administration of a Clostridial toxin
treatment is well tolerated. However, toxin administration in some
applications can be challenging because of the larger doses
required to achieve a beneficial effect. First, larger doses can
increase the likelihood that the toxin may move through the
interstitial fluids and the circulatory systems, such as, e.g., the
cardiovascular system and the lymphatic system, of the body,
resulting in the undesirable dispersal of the toxin to areas not
targeted for toxin treatment. Such dispersal can lead to
undesirable side effects, such as, e.g., inhibition of
neurotransmitter release in neurons not targeted for treatment or
paralysis of a muscle not targeted for treatment. For example, a
patient administered a therapeutically effective amount of a BoNT/A
treatment into the neck muscles for torticollis may develop
dysphagia because of dispersal of the toxin into the oropharynx.
Thus, there remains a need for improved Clostridial toxins that are
effective at the site of treatment, but have negligible to minimal
effects in areas not targeted for a toxin treatment.
[0005] Second, larger doses of a Clostridial toxin treatment may
elicit an antibody response against the toxin. While a potent and
effective treatment, the inhibition of neurotransmitter release and
the resulting neuromuscular paralysis elicited by Clostridial toxin
therapies is not permanent. The reversible nature of these
paralytic effects requires periodic treatments in order to maintain
the therapeutic benefits from this toxin. As a consequence of this
repeated exposure, an immune response against a Clostridial toxin
can occur in some patients which reduce or completely prevent the
individual's responsiveness to further treatments, see, e.g.,
Joseph Jankovic, Botulinum toxin: Clinical Implications of
Antigenicity and Immunoresistance, (SCIENTIFIC AND THERAPEUTIC
ASPECTS OF BOTULINUM TOXIN, 409-415, Mitchell F. Brin et al., eds.,
Lippincott Williams & Wilkins, 2002); Dirk Dressler, Clinical
Presentation and Management of Antibody-induced Failure of
Botulinum Toxin Therapy, 19(Suppl. 8) Mov. DISORD. S92-S100 (2004);
M. Zouhair Atassi, Basic Immunological Aspects of Botulinum Toxin
Therapy, 19(Suppl. 8) Mov. DISORD. S68-S84, (2004). Thus, there
remains a need for improved Clostridial toxins that maintain
effective therapeutic benefits, but have reduced ability to evoke
an immunogenic response against itself.
[0006] The growing clinical, therapeutic and cosmetic use of
Clostridial toxins in therapies requiring larger doses necessitates
the pharmaceutical industry to develop modified Clostridial toxins
that are effective at the target site of the application, but
reduce or prevent the undesirable side-effects associated with the
dispersal of the toxins to unwanted locations and reduce or prevent
an unwanted immunogenic response. One approach involves modifying a
Clostridial toxin so that the modified toxin has an enhanced cell
binding activity for a naturally-occurring Clostridial toxin
receptor. This enhanced binding activity is achieved by modifying
the endogenous targeting domain of a naturally-occurring
Clostridial toxin in order to enhance a cell binding activity of
the toxin for its naturally-occurring receptor. Such modifications
to a targeting domain result in, e.g., a enhanced cell binding
activity that increases binding affinity for an endogenous
Clostridial toxin receptor present on a naturally-occurring
Clostridial toxin target cell; an enhanced cell binding activity
that increases binding specificity for a subgroup of endogenous
Clostridial toxin receptors present on a naturally-occurring
Clostridial toxin target cell; or an enhanced cell binding activity
that increases both binding affinity and binding specificity. An
added advantage is achieved when the targeting domain is derived
from a human targeting domain polypeptide as these polypeptides as
less likely to elicit an immunogenic response in a patient. A
modified Clostridial toxin with an enhanced binding activity can
bind to a receptor, translocate into the cytoplasm, and exert its
proteolytic effect on the SNARE complex of the Clostridial toxin
target cell.
[0007] Non-limiting examples of modified Clostridial toxins an
enhanced cell binding activity for a naturally-occurring
Clostridial toxin receptor are described in, e.g., Lance E.
Steward, et al., Modified Clostridial Toxins with Enhanced
Targeting Capabilities For Endogenous Clostridial Toxin Receptors,
International Patent Application No. 2006/008956 (Mar. 14, 2006).
This enhanced binding activity for a naturally occurring
Clostridial toxin receptor allows for lower effective doses of a
modified Clostridial toxin to be administered to an individual
because more toxins will be delivered to the target cell. Thus,
modified Clostridial toxins with an enhanced cell binding activity
for a Clostridial toxin receptor will reduce the undesirable
dispersal of the toxin to areas not targeted for treatment, thereby
reducing or preventing the undesirable side-effects associated with
diffusion of a Clostridial toxin to an unwanted location.
[0008] The present invention provides novel modified Clostridial
toxins that reduce or prevent unwanted side-effects associated with
toxin dispersal into non-targeted areas. These modified Clostridial
toxins comprise, in part, a translocation facilitator domain that
enhances the process by which a light chain from a modified toxin
translocates into the cytoplasm of a target cell and enzymatically
modify its target SNARE substrate. Thus modified Clostridial toxins
with enhanced cell binding activity for its naturally-occurring
receptor, such as, e.g., those disclosed in Steward, supra, (2006),
comprising a translocation facilitator domain disclosed in the
present specification, exhibit increased potency at the Clostridial
toxin target cell and a concomitant reduction of unwanted
side-effects associated with toxin dispersal into non-targeted
areas. These and related advantages are useful for various
clinical, therapeutic and cosmetic applications, such as, e.g., the
treatment of neuromuscular disorders, neuropathic disorders, eye
disorders, pain, muscle injuries, headache, cardiovascular
diseases, neuropsychiatric disorders, endocrine disorders, cancers,
otic disorders and hyperkinetic facial lines, as well as, other
disorders where administration of a modified Clostridial toxin with
enhanced cell binding activity for a Clostridial toxin receptor to
an individual can produce a beneficial effect.
BRIEF DESCRIPTION OF THE DRAWINGS
[0009] FIG. 1 shows a schematic of the current paradigm of
neurotransmitter release and Clostridial toxin intoxication in a
central and peripheral neuron. FIG. 1A shows a schematic for the
neurotransmitter release mechanism of a central and peripheral
neuron. The release process can be described as comprising two
steps: 1) vesicle docking, where the vesicle-bound SNARE protein of
a vesicle containing neurotransmitter molecules associates with the
membrane-bound SNARE proteins located at the plasma membrane; and
2) neurotransmitter release, where the vesicle fuses with the
plasma membrane and the neurotransmitter molecules are exocytosed.
FIG. 1B shows a schematic of the intoxication mechanism for tetanus
and botulinum toxin activity in a central and peripheral neuron.
This intoxication process can be described as comprising four
steps: 1) receptor binding, where a Clostridial toxin binds to a
Clostridial receptor and initiates the intoxication process; 2)
complex internalization, where after toxin binding, a vesicle
containing the toxin/receptor complex is endocytosed into the cell;
3) light chain translocation, where multiple events result in the
release of the active light chain into the cytoplasm; and 4)
enzymatic target modification, where the active light chain of
Clostridial toxin proteolytically cleaves its target SNARE
substrate, such as, e.g., SNAP-25, VAMP or Syntaxin, thereby
preventing vesicle docking and neurotransmitter release.
[0010] FIG. 2 shows the domain organization of naturally-occurring
Clostridial toxins. The single chain form depicts the amino to
carboxyl linear organization comprising an enzymatic domain, a
translocation domain, a H.sub.CN translocation facilitating domain
and a H.sub.CC targeting domain. The di-chain loop region located
between the translocation and enzymatic domains is depicted by the
double SS bracket. This region comprises an endogenous di-chain
loop protease cleavage site that upon proteolytic cleavage with a
naturally-occurring protease, such as, e.g., an endogenous
Clostridial toxin protease or a naturally-occurring protease
produced in the environment, converts the single chain form of the
toxin into the di-chain form. As depicted above the single-chain
form, the H.sub.CC targeting domain comprises the .beta.-trefoil
domain which comprises in an amino to carboxyl linear organization
of an .alpha.-fold, a .beta.4/.beta.5 hairpin turn, a .beta.-fold,
a .beta.8/.beta.9 hairpin turn and a .gamma.-fold.
[0011] FIG. 3 shows a ribbon diagram of BoNT/A illustrating the
modular three-dimensional structure of the light chain (LC)
comprising the enzymatic domain, the heavy chain H.sub.N domain
comprising the translocation domain, the heavy chain H.sub.CN
domain comprising the translocation facilitating domain and the
heavy chain H.sub.CC domain comprising the targeting domain.
[0012] FIG. 4 shows modified Clostridial toxins with an enhanced
translocation capability and an enhanced targeting activity located
at the amino terminus of the modified toxin. FIG. 4A depicts the
single polypeptide form of a modified Clostridial toxin with an
amino to carboxyl linear organization comprising an enhanced
targeting domain, a translocation domain, a translocation
facilitating domain and an enzymatic domain, with the di-chain loop
region depicted by the double SS bracket. A proteolytic cleavage
site (P) within a di-chain loop region is located between the
translocation facilitating and enzymatic domains. Upon proteolytic
cleavage with a P protease, the single chain form of the toxin is
converted to the di-chain form. The P protease site can be a
Clostridial toxin endogenous protease cleavage site or a
non-Clostridial toxin exogenous protease cleavage site. Spacers can
be placed between the targeting and translocation domains, the
translocation and translocation facilitating domains, translocation
facilitating and enzymatic domains or any combination thereof. FIG.
4B depicts the single polypeptide form of a modified Clostridial
toxin with an amino to carboxyl linear organization comprising an
enhanced targeting domain, an enzymatic domain, a translocation
domain and a translocation facilitating domain, with the di-chain
loop region depicted by the double SS bracket. A proteolytic
cleavage site (P) within a di-chain loop region is located between
the enzymatic and translocation domains. Upon proteolytic cleavage
with a P protease, the single chain form of the toxin is converted
to the di-chain form. The P protease site can be a Clostridial
toxin endogenous protease cleavage site or a non-Clostridial toxin
exogenous protease cleavage site. Spacers can be placed between the
targeting and enzymatic domains, the enzymatic and translocation
domains, translocation and translocation facilitating domains or
any combination thereof.
[0013] FIG. 5 shows modified Clostridial toxins with an enhanced
translocation capability and an enhanced targeting activity located
between the two other domains. FIG. 5A depicts the single
polypeptide form of a modified Clostridial toxin with an amino to
carboxyl linear organization comprising an enzymatic domain, an
enhanced targeting domain, a translocation domain and a
translocation facilitating domain, with the di-chain loop region
depicted by the double SS bracket. A proteolytic cleavage site (P)
within a di-chain loop region is located between the enzymatic and
targeting domains. Upon proteolytic cleavage with a P protease, the
single chain form of the toxin is converted to the di-chain form.
The P protease site can be a Clostridial toxin endogenous protease
cleavage site or a non-Clostridial toxin exogenous protease
cleavage site. Spacers can be placed between the enzymatic and
targeting domains, the targeting and translocation domains, the
translocation and translocation facilitating domains or any
combination thereof. FIG. 5B depicts the single polypeptide form of
a modified Clostridial toxin with an amino to carboxyl linear
organization comprising a translocation domain, a translocation
facilitating domain, an enhanced targeting domain and an enzymatic
domain, with the di-chain loop region depicted by the double SS
bracket. A proteolytic cleavage site (P) within a di-chain loop
region is located between the translocation facilitating and
targeting domains. Upon proteolytic cleavage with a P protease, the
single chain form of the toxin is converted to the di-chain form.
The P protease site can be a Clostridial toxin endogenous protease
cleavage site or a non-Clostridial toxin exogenous protease
cleavage site. Spacers can be placed between the translocation and
translocation facilitating domains, the translocation facilitating
and targeting domains, the targeting and enzymatic domains or any
combination thereof.
[0014] FIG. 6 shows modified Clostridial toxins with an enhanced
translocation capability and an enhanced targeting activity located
at the carboxyl terminus of the modified toxin. FIG. 6A depicts the
single polypeptide form of a modified Clostridial toxin with an
amino to carboxyl linear organization comprising an enzymatic
domain, a translocation domain, a translocation facilitating domain
and an enhanced targeting domain, with the di-chain loop region
depicted by the double SS bracket. A proteolytic cleavage site (P)
within a di-chain loop region is located between the enzymatic and
translocation domains. Upon proteolytic cleavage with a P protease,
the single chain form of the toxin is converted to the di-chain
form. The P protease site can be a Clostridial toxin endogenous
protease cleavage site or a non-Clostridial toxin exogenous
protease cleavage site. Spacers can be placed between the enzymatic
and translocation domains, the translocation and translocation
facilitating domains, the translocation facilitating and targeting
domains or any combination thereof. FIG. 6B depicts the single
polypeptide form of a modified Clostridial toxin with an amino to
carboxyl linear organization comprising a translocation domain, a
translocation facilitating domain, an enzymatic domain and an
enhanced targeting domain, with the di-chain loop region depicted
by the double SS bracket. A proteolytic cleavage site (P) within a
di-chain loop region is located between the translocation
facilitating and enzymatic domains. Upon proteolytic cleavage with
a P protease, the single chain form of the toxin is converted to
the di-chain form. The P protease site can be a Clostridial toxin
endogenous protease cleavage site or a non-Clostridial toxin
exogenous protease cleavage site. Spacers can be placed between the
translocation and translocation facilitating domains, the
translocation facilitating and enzymatic domains, the enzymatic and
targeting domains or any combination thereof.
[0015] FIG. 7 shows a ribbon diagram of BoNT/A illustrating the
boundary regions of the H.sub.CN domain comprising the
translocation facilitating domain. FIG. 7A depicts the
amino-terminal boundary region. FIG. 7B depicts the
carboxyl-terminal boundary region.
DETAILED DESCRIPTION
[0016] Clostridia toxins produced by Clostridium botulinum,
Clostridium tetani, Clostridium baratii and Clostridium butyricum
are the most widely used in therapeutic and cosmetic treatments of
humans and other mammals. Strains of C. botulinum produce seven
antigenically-distinct types of Botulinum toxins (BoNTs), which
have been identified by investigating botulism outbreaks in man
(BoNT/A, /B, /E and /F), animals (BoNT/C1 and /D), or isolated from
soil (BoNT/G). While all seven botulinum toxins (BoNT) serotypes
have similar structure and pharmacological properties, each also
displays heterogeneous bacteriological characteristics. In
contrast, tetanus toxin (TeNT) is produced by a uniform group of C.
tetani. Two other species of Clostridia, C. baratii and C.
butyricum, also produce toxins similar to BoNT/F and BoNT/E,
respectively.
[0017] Clostridia toxins possess approximately 35% amino acid
identity with each other and share the same functional domain
organization and overall structural architecture. Clostridial
toxins are each translated as a single chain polypeptide of
approximately 150 kDa that is subsequently cleaved by proteolytic
scission within a disulfide loop by a naturally-occurring protease,
such as, e.g., an endogenous Clostridial toxin protease or a
naturally-occurring protease produced in the environment (FIG. 2).
This posttranslational processing yields a di-chain molecule
comprising an approximately 50 kDa light chain (LC) and an
approximately 100 kDa heavy chain (HC) held together by a single
disulfide bond and noncovalent interactions. It is widely held that
the mature di-chain molecule comprises three functionally distinct
domains: 1) an enzymatic domain located in the LC that includes a
metalloprotease region containing a zinc-dependent endopeptidase
activity which specifically targets core components of the
neurotransmitter release apparatus (Table 1); 2) a translocation
domain contained within the amino-terminal half of the heavy chain
(H.sub.N domain) that facilitates release of the LC from
intracellular vesicles into the cytoplasm of the target cell (Table
1); and 3) a binding domain found within the carboxyl-terminal half
of the heavy chain (H.sub.C domain) that determines the binding
activity and binding specificity of the toxin to the receptor
complex located at the surface of the target cell (Table 1), see,
e.g., Kathryn Turton et al., Botulinum and Tetanus Neurotoxins:
Structure, Function and Therapeutic Utility, 27(11) Trends Biochem.
Sci. 552-558. (2002); John A. Chaddock and P. M. H. Marks,
Clostridial Neurotoxins: Structure-Function Led Design of New
Therapeutics, 63(5) Cell. Mol. Life. Sci. 540-551 (2006); and Keith
Foster et al., Re-engineering the Target Specificity of Clostridial
Neurotoxins--A Route To Novel Therapeutics, 9(2-3) Neurotox Res.
101-107 (2006).
TABLE-US-00001 TABLE 1 Clostridial Toxin Reference Sequences and
Regions SEQ H.sub.C Toxin ID NO: LC H.sub.N H.sub.CN H.sub.CC
BoNT/A 1 M1-K448 A449-I873 I874-P1110 Y1111-L1296 BoNT/B 2 M1-K441
A442-I860 L861-E1097 Y1098-E1291 BoNT/C1 3 M1-K449 T450-I868
N869-E1111 Y1112-E1291 BoNT/D 4 M1-R445 D446-I864 N865-E1098
Y1099-E1276 BoNT/E 5 M1-R422 K423-I847 K848-E1085 Y1086-K1252
BoNT/F 6 M1-K439 A440-I866 K867-K1105 Y1106-E1274 BoNT/G 7 M1-K446
S447-I865 S866-Q1105 Y1106-E1297 TeNT 8 M1-A457 S458-L881
K882-E1127 Y1128-D1315
[0018] The binding, translocation and enzymatic activities of a
Clostridial toxin are all necessary to execute the overall cellular
intoxication mechanism whereby Clostridial toxins enter a neuron
and inhibit neurotransmitter release is similar, regardless of
serotype or subtype. The current paradigm describes the
intoxication mechanism as comprising at least four steps: 1)
receptor binding, 2) complex internalization, 3) light chain
translocation, and 4) enzymatic target modification (see FIG. 1).
The process is initiated when the H.sub.C domain of a Clostridial
toxin binds to a toxin-specific receptor located on the plasma
membrane surface of a target cell. The binding specificity of a
receptor complex is thought to be achieved, in part, by specific
combinations of gangliosides and protein receptors that appear to
distinctly comprise each Clostridial toxin receptor complex. Once
bound, the toxin/receptor complexes are internalized by endocytosis
and the internalized vesicles are sorted to specific intracellular
routes. The translocation step, now thought to be mediated by the
H.sub.N domain and further facilitated by the H.sub.CN domain,
appears to be triggered by the acidification of the vesicle
compartment. This process seems to initiate two important
pH-dependent structural rearrangements that increase hydrophobicity
and promote separation of the light chain from the heavy chain of
the toxin. Once activated, light chain endopeptidase of the toxin
is released from the intracellular vesicle into the cytosol where
it appears to specifically target one of three known core
components of the neurotransmitter release apparatus. These core
proteins, vesicle-associated membrane protein (VAMP)/synaptobrevin,
synaptosomal-associated protein of 25 kDa (SNAP-25) and Syntaxin,
are necessary for synaptic vesicle docking and fusion at the nerve
terminal and constitute members of the soluble
N-ethylmaleimide-sensitive factor-attachment protein-receptor
(SNARE) family. BoNT/A and BoNT/E cleave SNAP-25 in the
carboxyl-terminal region, releasing a nine or twenty-six amino acid
segment, respectively, and BoNT/C1 also cleaves SNAP-25 near the
carboxyl-terminus. The botulinum serotypes BoNT/B, BoNT/D, BoNT/F
and BoNT/G, and tetanus toxin, act on the conserved central portion
of VAMP, and release the amino-terminal portion of VAMP into the
cytosol. BoNT/C1 cleaves syntaxin at a single site near the
cytosolic membrane surface. The selective proteolysis of synaptic
SNAREs accounts for the block of neurotransmitter release caused by
Clostridial toxins in vivo. The SNARE protein targets of
Clostridial toxins are common to exocytosis in a variety of
non-neuronal types; in these cells, as in neurons, light chain
peptidase activity inhibits exocytosis, see, e.g., Yann Humeau et
al., How Botulinum and Tetanus Neurotoxins Block Neurotransmitter
Release, 82(5) Biochimie. 427-446 (2000); and Giovanna Lalli et
al., The Journey of Tetanus and Botulinum Neurotoxins in Neurons,
11(9) Trends Microbiol. 431-437, (2003).
[0019] The three-dimensional crystal structures of BoNT/A, BoNT/B
and the H.sub.C domain of TeNT indicate that the three functional
domains of Clostridial neurotoxins are structurally distinct. The
HEXXH consensus motif of the light chain forms the tetrahedral zinc
binding pocket of the catalytic site located in a deep cleft on the
protein surface that is accessible by a channel. The structure of
the H.sub.N and H.sub.C domains consists primarily of .beta.-sheet
topologies that are linked by a single .alpha.-helix. The
cylindrical-shaped H.sub.N domain comprises two long amphipathic
.alpha.-helices that resemble the coiled-coil motif found in some
viral proteins. The H.sub.N domain also forms a long unstructured
loop called the `translocation belt,` which wraps around a large
negatively charged cleft of the light chain that blocks access of
the zinc atom to the catalytic-binding pocket of active site. The
H.sub.C domain comprises two distinct structural features of
roughly equal size that indicate function. The first, designated
the H.sub.CN domain, is located in the amino half of the H.sub.C
domain. The H.sub.CN domain forms a .beta.-barrel, jelly-roll fold.
The H.sub.CC domain is the second domain that comprises the H.sub.C
domain. This carboxyl-terminal domain comprises a modified
.beta.-trefoil domain which forms three distinct carbohydrate
binding regions that resembles the carbohydrate binding moiety
found in many sugar-binding proteins, such as, e.g., serum amyloid
P, sialidase, cryia, insecticidal .delta.-endotoxin and lectins.
Biochemical studies indicate that the .beta.-trefoil domain
structure of the H.sub.CC domain appears to mediate the binding to
specific carbohydrate containing components of the Clostridial
toxin receptor on the cell surface, see, e.g., Krzysztof Ginalski
et al., Structure-based Sequence Alignment for the Beta-Trefoil
Subdomain of the Clostridial Neurotoxin Family Provides Residue
Level Information About the Putative Ganglioside Binding Site,
482(1-2) FEBS Lett. 119-124 (2000). The H.sub.C domain tilts away
from the H.sub.N domain exposing the surface loops and making them
accessible for binding. No contacts occur between the light chain
and the H.sub.C domain.
[0020] We know that only the H.sub.CC domain participates in
receptor binding because the .beta.-trefoil domains are restricted
to this domain. Proteins containing the structural .beta.-trefoil
domain represents a diverse group of proteins organized into at
least eight superfamilies including the cytokines, MIR domain
proteins, Ricin B-like lectins, agglutinins, Soybean trypsin
inhibitor like proteins, Actin-crosslinking proteins, LAG-1
proteins and AbfB domain proteins, see, e.g., C. A. Orengo et al.,
Protein Superfamilies and Domain Superfolds, 372 Nature 631-634
(1994); and Alexey G. Murzin et al., SCOP: A Structural
Classification of Proteins Database for the Investigation of
Sequences and Structures, 247(4) J. Mol. Biol. 536-540 (1995).
While having diverse cellular roles, members of these superfamilies
mechanistically function via protein-protein associations through
the .beta.-trefoil domain. Of particular interest is the fact that
many of these members are specifically involved in receptor
interactions, including, e.g., the cytokine superfamily members
Fibroblast Growth Factors (FGFs) and the Interleukin-1s (IL-1s);
the Ricin B-like lectins; the agglutinins; and STI-like members the
Kunitz inhibitors and Clostridium neurotoxins. That only the
H.sub.CC domain alone mediates the cell binding step of
intoxication is further supported by the finding that mutations
that disrupt the receptor binding activity of Clostridial toxins
have been confined to the H.sub.CC domain, see, e.g., Andreas
Rummel et al., The H.sub.CC-Domain of Botulinum Neurotoxins A and B
Exhibits a Singular Ganglioside Binding Site Displaying Serotype
Specific Carbohydrate Interaction, 51.beta.) Mol. Microbiol.
631-643 (2004).
[0021] Because the H.sub.CC domain appears not only necessary, but
sufficient for selective binding of a Clostridial toxin to its
receptor, we have deduced that the primary function of the H.sub.CN
domain of Clostridial toxins is involved in the translocation step
of the intoxication process, and not in the cell binding step,
because the lack of H.sub.CN domain appears to reduce intoxication
efficiency. For example, a modified BoNT/A comprising a Substance P
targeting domain was inefficient in intoxicating its corresponding
target cells. In this modified toxin, the entire BoNT/A H.sub.C
domain, comprising both the BoNT/A H.sub.CC domain and the BoNT/A
H.sub.CN domain, was replaced by the Substance P targeting domain.
Likewise, we have determined that several other modified
Clostridial toxins that have replaced the entire BoNT/A H.sub.e
domain with an exogenous targeting domain have exhibited reduced
intoxication capabilities. Thus, the H.sub.CN domain possess a
translocation facilitating function because 1) H.sub.CC domain
primarily mediates the receptor binding step of the intoxication
process; 2) modified Clostridial toxins lacking the H.sub.CN domain
exhibit a reduced ability to translocate into the cytoplasm as
evident by such modified toxins exhibiting decreased proteolysis of
their SNARE substrates; and 3) the LC domain mediates the enzymatic
activity of the toxin. While the exact translocation facilitating
mechanism of the H.sub.CN domain is currently not understood, the
H.sub.CN domain may 1) participate in the formation of an endosomal
pore; 2) mediate the insertion of the pore into a vesicle membrane;
3) assist in the delivery of LC across the endosomal membrane
and/or 4) serve as a structural scaffold or spacer that facilitates
the appropriate orientation a the targeting domain in relationship
to the translocation domain. In this last point, the H.sub.CN
domain would serve to orient the translocation domain to facilitate
the proper presentation of the translocation domain for insertion
into the membrane following binding of the ligand by the receptor.
This novel role of the H.sub.CN domain in the translocation step is
contrary to the widely accepted view that the Clostridial toxin
H.sub.CN domain played an integral role in the cell binding step of
the intoxication process.
[0022] Thus, the present invention discloses modified Clostridial
toxins that exhibit 1) an enhanced translocation capability; and 2)
an enhanced targeting capability for a naturally-occurring
Clostridial toxin receptor. The enhanced translocation capability
is mediated by a translocation facilitating domain comprising,
e.g., a H.sub.CN region of Clostridial toxins. The H.sub.CN domain
enhances the process by which the H.sub.N domain mediates the
release of the light chain from internalized intracellular vesicles
into the cytoplasm of the target cell during the translocation
step. Enhanced translocation capability is obtained by including or
maintaining a Clostridial toxin H.sub.CN domain in a modified
Clostridial toxin disclosed in the present specification. The
enhanced targeting capability for a naturally-occurring Clostridial
toxin receptor is mediated by an enhanced targeting domain
comprising a modified H.sub.CC targeting domain of Clostridial
toxins. The H.sub.CC domain primarily determines the binding
activity and binding specificity of the toxin to the receptor
complex located at the surface of the target cell. Enhanced binding
activity is achieved by replacing the endogenous H.sub.CC targeting
domain of a Clostridial toxin with a targeting domain showing
enhanced binding activity for a Clostridial toxin receptor present
on a naturally-occurring Clostridial toxin target cell.
[0023] Both the enhanced translocation and enhanced targeting
activity should allow lower effective doses of a modified
Clostridial toxin to be administered to an individual because more
toxin will be delivered to a target cell. Thus modified Clostridial
toxins with enhanced translocation and binding capabilities will
reduce the undesirable dispersal of the toxin to areas not targeted
for treatment, thereby reducing or preventing the undesirable
side-effects associated with diffusion of a Clostridial toxin to an
unwanted location.
[0024] Thus, aspects of the present invention provide modified
Clostridial toxins comprising a Clostridial toxin enzymatic domain,
a Clostridial toxin translocation domain, a translocation
facilitating domain and an enhanced targeting domain, wherein the
modified Clostridial toxin exhibits an enhanced targeting activity
for a Clostridial toxin receptor as compared to a
naturally-occurring Clostridial toxin. It is envisioned that any
translocation facilitating domain capable of further facilitating
the translocation step of the intoxication process where the light
chain is released from intracellular vesicles into the cytoplasm of
the target cell will be useful to practice aspects of the present
invention, including, without limitation, a Clostridial toxin
translocation facilitating domain and an enveloped virus fusogenic
peptide domain. Likewise, a multitude of enhanced targeting domains
are envisioned, including, without limitation, a modified
Clostridial toxin targeting domain with enhanced binding activity,
such as, e.g., a BoNT/A targeting domain with enhanced binding
activity, a BoNT/B targeting domain with enhanced binding activity,
a BoNT/C1 targeting domain with enhanced binding activity, a BoNT/D
targeting domain with enhanced binding activity, a BoNT/E targeting
domain with enhanced binding activity, a BoNT/F targeting domain
with enhanced binding activity, a BoNT/G targeting domain with
enhanced binding activity, a TeNT targeting domain with enhanced
binding activity, or active fragment thereof; a Clostridial NAP,
such as, e.g., a Clostridial botulinum serotype A HA-33, a
Clostridial botulinum serotype B HA-33, a Clostridial botulinum
serotype C1 HA-33, a Clostridial botulinum serotype D HA-33, a
Clostridial botulinum serotype A HA-17, a Clostridial botulinum
serotype B HA-17, a Clostridial botulinum serotype C1 HA-17, a
Clostridial botulinum serotype D HA-17, a Clostridial botulinum
serotype A NTNH, a Clostridial botulinum serotype B NTNH, a
Clostridial botulinum serotype C1 NTNH, a Clostridial botulinum
serotype D NTNH, a Clostridial botulinum serotype E NTNH, a
Clostridial botulinum serotype F NTNH and a Clostridial botulinum
serotype G NTNH; and a FGF, such as, e.g., a FGF-1, a FGF-2, a
FGF-4, a FGF-8, a FGF-9, a FGF-17 and a FGF-18. It is also
envisioned that the location of the enhanced targeting domain in
the modified Clostridial toxins of the present specification can be
located at the amino terminus of the toxin, between the enzymatic
and translocation domains or at the carboxyl terminus of the toxin.
Thus, a modified Clostridial toxins disclosed in the present
specification can comprise an amino to carboxyl domain arrangement
of, e.g., an enhanced targeting domain, a Clostridial toxin
translocation domain, a translocation facilitating domain and a
Clostridial toxin enzymatic domain; an enhanced targeting domain, a
Clostridial toxin enzymatic domain, a Clostridial toxin
translocation domain and a translocation facilitating domain; a
Clostridial toxin enzymatic domain, an enhanced targeting domain, a
Clostridial toxin translocation domain and a translocation
facilitating domain; a Clostridial toxin translocation domain, a
translocation facilitating domain, an enhanced targeting domain and
a Clostridial toxin enzymatic domain; a Clostridial toxin enzymatic
domain, a Clostridial toxin translocation domain, a translocation
facilitating domain and an enhanced targeting domain; and a
Clostridial toxin translocation domain, a translocation
facilitating domain, a Clostridial toxin enzymatic domain and an
enhanced targeting domain.
[0025] Other aspects of the present invention provide
polynucleotide molecules encoding modified Clostridial toxins
comprising a Clostridial toxin enzymatic domain, a Clostridial
toxin translocation domain, a translocation facilitating domain and
an enhanced targeting domain, wherein the modified Clostridial
toxin exhibits an enhanced targeting activity for a Clostridial
toxin receptor as compared to a naturally-occurring Clostridial
toxin. It is envisioned that the location of the enhanced targeting
domain of the modified Clostridial toxins encoded by polynucleotide
molecules of the present specification can be located at the amino
terminus of the toxin, between the enzymatic and translocation
domains or at the carboxyl terminus of the toxin.
[0026] Other aspects of the present invention provide methods of
producing a modified Clostridial toxin disclosed in the present
specification, the method comprising the step of expressing in a
cell a polynucleotide molecule encoding a modified Clostridial
toxin comprising a Clostridial toxin enzymatic domain, a
Clostridial toxin translocation domain, a translocation
facilitating domain and an enhanced targeting domain, wherein the
modified Clostridial toxin exhibits an enhanced targeting activity
for a Clostridial toxin receptor as compared to a
naturally-occurring Clostridial toxin. Other aspects of the present
invention provide methods of producing a modified Clostridial toxin
disclosed in the present specification, the method comprising the
steps of introducing in a cell an expression construct comprising a
polynucleotide molecule encoding a modified Clostridial toxin
comprising a Clostridial toxin enzymatic domain, a Clostridial
toxin translocation domain, a translocation facilitating domain and
an enhanced targeting domain, wherein the modified Clostridial
toxin exhibits an enhanced targeting activity for a Clostridial
toxin receptor as compared to a naturally-occurring Clostridial
toxin and expressing the expression construct in the cell.
[0027] Aspects of the present invention provide, in part, a
modified Clostridial toxin. As used herein, the term "modified
Clostridial toxin" means any polypeptide that can execute the
overall cellular mechanism whereby a Clostridial toxin enters a
neuron and inhibits neurotransmitter release and encompasses the
binding of a Clostridial toxin to a low or high affinity receptor
complex, the internalization of the toxin, the translocation of the
Clostridial toxin light chain into the cytoplasm and the enzymatic
modification of a Clostridial toxin substrate. A modified
Clostridial toxin disclosed in the present specification is
distinguished from a naturally-occurring Clostridial toxin by the
fact that a modified Clostridial toxin comprises a translocation
facilitating domain that enhances the process by which a light
chain from a modified toxin translocates into the cytoplasm of a
target cell and a modified Clostridial toxin lacks the cell binding
activity of a naturally-occurring binding domain found in a
Clostridial toxin. Instead, a modified Clostridial toxin disclosed
in the present specification comprises an enhanced targeting domain
that determines the binding activity of the modified Clostridial
toxin to an endogenous Clostridial toxin receptor located at the
surface of the target cell. By definition, a naturally-occurring
Clostridial toxin lacks an enhanced targeting domain. Examples of
modified Clostridial toxin are described in, e.g., Lance E.
Steward, et al., Modified Clostridial Toxins with Enhanced
Targeting Capabilities For Endogenous Clostridial Toxin Receptor
Systems, International Patent Application No. 2006/008956 (Mar. 14,
2006). Any of the modified Clostridial toxins described in, e.g.,
Steward, supra, (Mar. 14, 2006), can be further modified to include
a translocation facilitating domain as disclosed in the present
specification.
[0028] Aspects of the present invention provide, in part, a
Clostridial toxin enzymatic domain. As used herein, the term
"Clostridial toxin enzymatic domain" means any Clostridial toxin
polypeptide that can execute the enzymatic target modification step
of the intoxication process. Thus, a Clostridial toxin enzymatic
domain specifically targets a Clostridial toxin substrate and
encompasses the proteolytic cleavage of a Clostridial toxin
substrate, such as, e.g., SNARE proteins like a SNAP-25 substrate,
a VAMP substrate and a Syntaxin substrate. Non-limiting examples of
a Clostridial toxin enzymatic domain include, e.g., a Clostridial
toxin light chain region such as, e.g., a BoNT/A light chain
region, a BoNT/B light chain region, a BoNT/C1 light chain region,
a BoNT/D light chain region, a BoNT/E light chain region, a BoNT/F
light chain region, a BoNT/G light chain region, and a TeNT light
chain region.
[0029] A Clostridial toxin enzymatic domain includes, without
limitation, naturally occurring Clostridial toxin light chain
variants, such as, e.g., Clostridial toxin light chain isoforms and
Clostridial toxin light chain subtypes; non-naturally occurring
Clostridial toxin light chain variants, such as, e.g., conservative
Clostridial toxin light chain variants, non-conservative
Clostridial toxin light chain variants, Clostridial toxin light
chain chimerics, active Clostridial toxin light chain fragments
thereof, or any combination thereof.
[0030] As used herein, the term "Clostridial toxin light chain
variant," whether naturally-occurring or non-naturally-occurring,
means a Clostridial toxin light chain that has at least one amino
acid change from the corresponding region of the disclosed
reference sequences (see Table 1) and can be described in percent
identity to the corresponding region of that reference sequence.
Unless expressly indicated, all Clostridial toxin light chain
variants disclosed in the present specification are capable of
executing the enzymatic target modification step of the
intoxication process. As non-limiting examples, a BoNT/A light
chain variant comprising amino acids 1-448 of SEQ ID NO: 1 will
have at least one amino acid difference, such as, e.g., an amino
acid substitution, deletion or addition, as compared to the amino
acid region 1-448 of SEQ ID NO: 1; a BoNT/B light chain variant
comprising amino acids 1-441 of SEQ ID NO: 2 will have at least one
amino acid difference, such as, e.g., an amino acid substitution,
deletion or addition, as compared to the amino acid region 1-441 of
SEQ ID NO: 2; a BoNT/C1 light chain variant comprising amino acids
1-449 of SEQ ID NO: 3 will have at least one amino acid difference,
such as, e.g., an amino acid substitution, deletion or addition, as
compared to the amino acid region 1-449 of SEQ ID NO: 3; a BoNT/D
light chain variant comprising amino acids 1-445 of SEQ ID NO: 4
will have at least one amino acid difference, such as, e.g., an
amino acid substitution, deletion or addition, as compared to the
amino acid region 1-445 of SEQ ID NO: 4; a BoNT/E light chain
variant comprising amino acids 1-422 of SEQ ID NO: 5 will have at
least one amino acid difference, such as, e.g., an amino acid
substitution, deletion or addition, as compared to the amino acid
region 1-422 of SEQ ID NO: 5; a BoNT/F light chain variant
comprising amino acids 1-439 of SEQ ID NO: 6 will have at least one
amino acid difference, such as, e.g., an amino acid substitution,
deletion or addition, as compared to the amino acid region 1-439 of
SEQ ID NO: 6; a BoNT/G light chain variant comprising amino acids
1-446 of SEQ ID NO: 7 will have at least one amino acid difference,
such as, e.g., an amino acid substitution, deletion or addition, as
compared to the amino acid region 1-446 of SEQ ID NO: 7; and a TeNT
light chain variant comprising amino acids 1-457 of SEQ ID NO: 8
will have at least one amino acid difference, such as, e.g., an
amino acid substitution, deletion or addition, as compared to the
amino acid region 1-457 of SEQ ID NO: 8.
[0031] It is recognized by those of skill in the art that within
each serotype of Clostridial toxin there can be naturally occurring
Clostridial toxin light chain variants that differ somewhat in
their amino acid sequence, and also in the nucleic acids encoding
these proteins. For example, there are presently four BoNT/A
subtypes, BoNT/A1, BoNT/A2, BoNT/A3 and BoNT/A4, with specific
light chain subtypes showing approximately 95% amino acid identity
when compared to another BoNT/A light chain subtype. As used
herein, the term "naturally occurring Clostridial toxin light chain
variant" means any Clostridial toxin light chain produced by a
naturally-occurring process, including, without limitation,
Clostridial toxin light chain isoforms produced from
alternatively-spliced transcripts, Clostridial toxin light chain
isoforms produced by spontaneous mutation and Clostridial toxin
light chain subtypes. A naturally occurring Clostridial toxin light
chain variant can function in substantially the same manner as the
reference Clostridial toxin light chain on which the naturally
occurring Clostridial toxin light chain variant is based, and can
be substituted for the reference Clostridial toxin light chain in
any aspect of the present invention. A naturally occurring
Clostridial toxin light chain variant may substitute one or more
amino acids, two or more amino acids, three or more amino acids,
four or more amino acids, five or more amino acids, ten or more
amino acids, 20 or more amino acids, 30 or more amino acids, 40 or
more amino acids, 50 or more amino acids or 100 or more amino acids
from the reference Clostridial toxin light chain on which the
naturally occurring Clostridial toxin light chain variant is based.
A naturally occurring Clostridial toxin light chain variant can
also substitute at least 10 contiguous amino acids, at least 15
contiguous amino acids, at least 20 contiguous amino acids, or at
least 25 contiguous amino acids from the reference Clostridial
toxin light chain on which the naturally occurring Clostridial
toxin light chain variant is based, that possess at least 50% amino
acid identity, 65% amino acid identity, 75% amino acid identity,
85% amino acid identity or 95% amino acid identity to the reference
Clostridial toxin light chain on which the naturally occurring
Clostridial toxin light chain variant is based.
[0032] A non-limiting examples of a naturally occurring Clostridial
toxin light chain variant is a Clostridial toxin light chain
isoform such as, e.g., a BoNT/A light chain isoform, a BoNT/B light
chain isoform, a BoNT/C1 light chain isoform, a BoNT/D light chain
isoform, a BoNT/E light chain isoform, a BoNT/F light chain
isoform, a BoNT/G light chain isoform, and a TeNT light chain
isoform. A Clostridial toxin light chain isoform can function in
substantially the same manner as the reference Clostridial toxin
light chain on which the Clostridial toxin light chain isoform is
based, and can be substituted for the reference Clostridial toxin
light chain in any aspect of the present invention.
[0033] Another non-limiting examples of a naturally occurring
Clostridial toxin light chain variant is a Clostridial toxin light
chain subtype such as, e.g., a light chain from subtype BoNT/A1,
BoNT/A2, BoNT/A3 and BoNT/A4; a light chain from subtype BoNT/B1,
BoNT/B2, BoNT/B bivalent and BoNT/B nonproteolytic; a light chain
from subtype BoNT/C1-1 and BoNT/C1-2; a light chain from subtype
BoNT/E1, BoNT/E2 and BoNT/E3; and a light chain from subtype
BoNT/F1, BoNT/F2, BoNT/F3 and BoNT/F4. A Clostridial toxin light
chain subtype can function in substantially the same manner as the
reference Clostridial toxin light chain on which the Clostridial
toxin light chain subtype is based, and can be substituted for the
reference Clostridial toxin light chain in any aspect of the
present invention.
[0034] As used herein, the term "non-naturally occurring
Clostridial toxin light chain variant" means any Clostridial toxin
light chain produced with the aid of human manipulation, including,
without limitation, Clostridial toxin light chains produced by
genetic engineering using random mutagenesis or rational design and
Clostridial toxin light chains produced by chemical synthesis.
Non-limiting examples of non-naturally occurring Clostridial toxin
light chain variants include, e.g., conservative Clostridial toxin
light chain variants, non-conservative Clostridial toxin light
chain variants, Clostridial toxin light chain chimeric variants and
active Clostridial toxin light chain fragments.
[0035] As used herein, the term "conservative Clostridial toxin
light chain variant" means a Clostridial toxin light chain that has
at least one amino acid substituted by another amino acid or an
amino acid analog that has at least one property similar to that of
the original amino acid from the reference Clostridial toxin light
chain sequence (Table 1). Examples of properties include, without
limitation, similar size, topography, charge, hydrophobicity,
hydrophilicity, lipophilicity, covalent-bonding capacity,
hydrogen-bonding capacity, a physicochemical property, of the like,
or any combination thereof. A conservative Clostridial toxin light
chain variant can function in substantially the same manner as the
reference Clostridial toxin light chain on which the conservative
Clostridial toxin light chain variant is based, and can be
substituted for the reference Clostridial toxin light chain in any
aspect of the present invention. A conservative Clostridial toxin
light chain variant may substitute one or more amino acids, two or
more amino acids, three or more amino acids, four or more amino
acids, five or more amino acids, ten or more amino acids, 20 or
more amino acids, 30 or more amino acids, 40 or more amino acids,
50 or more amino acids, 100 or more amino acids or 200 or more
amino acids from the reference Clostridial toxin light chain on
which the conservative Clostridial toxin light chain variant is
based. A conservative Clostridial toxin light chain variant can
also substitute at least 10 contiguous amino acids, at least 15
contiguous amino acids, at least 20 contiguous amino acids, or at
least 25 contiguous amino acids from the reference Clostridial
toxin light chain on which the conservative Clostridial toxin light
chain variant is based, that possess at least 50% amino acid
identity, 65% amino acid identity, 75% amino acid identity, 85%
amino acid identity or 95% amino acid identity to the reference
Clostridial toxin light chain on which the conservative Clostridial
toxin light chain variant is based. Non-limiting examples of a
conservative Clostridial toxin light chain variant include, e.g.,
conservative BoNT/A light chain variants, conservative BoNT/B light
chain variants, conservative BoNT/C1 light chain variants,
conservative BoNT/D light chain variants, conservative BoNT/E light
chain variants, conservative BoNT/F light chain variants,
conservative BoNT/G light chain variants, and conservative TeNT
light chain variants.
[0036] As used herein, the term "non-conservative Clostridial toxin
light chain variant" means a Clostridial toxin light chain in which
1) at least one amino acid is deleted from the reference
Clostridial toxin light chain on which the non-conservative
Clostridial toxin light chain variant is based; 2) at least one
amino acid added to the reference Clostridial toxin light chain on
which the non-conservative Clostridial toxin light chain is based;
or 3) at least one amino acid is substituted by another amino acid
or an amino acid analog that does not share any property similar to
that of the original amino acid from the reference Clostridial
toxin light chain sequence (Table 1). A non-conservative
Clostridial toxin light chain variant can function in substantially
the same manner as the reference Clostridial toxin light chain on
which the non-conservative Clostridial toxin light chain variant is
based, and can be substituted for the reference Clostridial toxin
light chain in any aspect of the present invention. A
non-conservative Clostridial toxin light chain variant can delete
one or more amino acids, two or more amino acids, three or more
amino acids, four or more amino acids, five or more amino acids,
and ten or more amino acids from the reference Clostridial toxin
light chain on which the non-conservative Clostridial toxin light
chain variant is based. A non-conservative Clostridial toxin light
chain variant can add one or more amino acids, two or more amino
acids, three or more amino acids, four or more amino acids, five or
more amino acids, and ten or more amino acids to the reference
Clostridial toxin light chain on which the non-conservative
Clostridial toxin light chain variant is based. A non-conservative
Clostridial toxin light chain variant may substitute one or more
amino acids, two or more amino acids, three or more amino acids,
four or more amino acids, five or more amino acids, ten or more
amino acids, 20 or more amino acids, 30 or more amino acids, 40 or
more amino acids, 50 or more amino acids, 100 or more amino acids
or 200 or more amino acids from the reference Clostridial toxin
light chain on which the non-conservative Clostridial toxin light
chain variant is based. A non-conservative Clostridial toxin light
chain variant can also substitute at least 10 contiguous amino
acids, at least 15 contiguous amino acids, at least 20 contiguous
amino acids, or at least 25 contiguous amino acids from the
reference Clostridial toxin light chain on which the
non-conservative Clostridial toxin light chain variant is based,
that possess at least 50% amino acid identity, 65% amino acid
identity, 75% amino acid identity, 85% amino acid identity or 95%
amino acid identity to the reference Clostridial toxin light chain
on which the non-conservative Clostridial toxin light chain variant
is based. Non-limiting examples of a non-conservative Clostridial
toxin light chain variant include, e.g., non-conservative BoNT/A
light chain variants, non-conservative BoNT/B light chain variants,
non-conservative BoNT/C1 light chain variants, non-conservative
BoNT/D light chain variants, non-conservative BoNT/E light chain
variants, non-conservative BoNT/F light chain variants,
non-conservative BoNT/G light chain variants, and non-conservative
TeNT light chain variants.
[0037] As used herein, the term "Clostridial toxin light chain
chimeric" means a polypeptide comprising at least a portion of a
Clostridial toxin light chain and at least a portion of at least
one other polypeptide to form a toxin light chain with at least one
property different from the reference Clostridial toxin light
chains of Table 1, with the proviso that this Clostridial toxin
light chain chimeric is still capable of specifically targeting the
core components of the neurotransmitter release apparatus and thus
participate in executing the overall cellular mechanism whereby a
Clostridial toxin proteolytically cleaves a substrate. Such
Clostridial toxin light chain chimerics are described in, e.g.,
Lance E. Steward et al., Leucine-based Motif and Clostridial
Toxins, U.S. Patent Publication 2003/0027752 (Feb. 6, 2003); Lance
E. Steward et al., Clostridial Neurotoxin Compositions and Modified
Clostridial Neurotoxins, U.S. Patent Publication 2003/0219462 (Nov.
27, 2003); and Lance E. Steward et al., Clostridial Neurotoxin
Compositions and Modified Clostridial Neurotoxins, U.S. Patent
Publication 2004/0220386 (Nov. 4, 2004).
[0038] As used herein, the term "active Clostridial toxin light
chain fragment" means any of a variety of Clostridial toxin
fragments comprising the light chain can be useful in aspects of
the present invention with the proviso that these light chain
fragments can specifically target the core components of the
neurotransmitter release apparatus and thus participate in
executing the overall cellular mechanism whereby a Clostridial
toxin proteolytically cleaves a substrate. The light chains of
Clostridial toxins are approximately 420-460 amino acids in length
and comprise an enzymatic domain (Table 1). Research has shown that
the entire length of a Clostridial toxin light chain is not
necessary for the enzymatic activity of the enzymatic domain. As a
non-limiting example, the first eight amino acids of the BoNT/A
light chain (residues 1-8 of SEQ ID NO: 1) are not required for
enzymatic activity. As another non-limiting example, the first
eight amino acids of the TeNT light chain (residues 1-8 of SEQ ID
NO: 8) are not required for enzymatic activity. Likewise, the
carboxyl-terminus of the light chain is not necessary for activity.
As a non-limiting example, the last 32 amino acids of the BoNT/A
light chain (residues 417-448 of SEQ ID NO: 1) are not required for
enzymatic activity. As another non-limiting example, the last 31
amino acids of the TeNT light chain (residues 427-457 of SEQ ID NO:
8) are not required for enzymatic activity. Thus, aspects of this
embodiment can include Clostridial toxin light chains comprising an
enzymatic domain having a length of, e.g., at least 350 amino
acids, at least 375 amino acids, at least 400 amino acids, at least
425 amino acids and at least 450 amino acids. Other aspects of this
embodiment can include Clostridial toxin light chains comprising an
enzymatic domain having a length of, e.g., at most 350 amino acids,
at most 375 amino acids, at most 400 amino acids, at most 425 amino
acids and at most 450 amino acids.
[0039] Any of a variety of sequence alignment methods can be used
to determine percent identity of naturally-occurring Clostridial
toxin light chain variants and non-naturally-occurring Clostridial
toxin light chain variants, including, without limitation, global
methods, local methods and hybrid methods, such as, e.g., segment
approach methods. Protocols to determine percent identity are
routine procedures within the scope of one skilled in the art and
from the teaching herein.
[0040] Global methods align sequences from the beginning to the end
of the molecule and determine the best alignment by adding up
scores of individual residue pairs and by imposing gap penalties.
Non-limiting methods include, e.g., CLUSTAL W, see, e.g., Julie D.
Thompson et al., CLUSTAL W: Improving the Sensitivity of
Progressive Multiple Sequence Alignment Through Sequence Weighting,
Position-Specific Gap Penalties and Weight Matrix Choice, 22(22)
Nucleic Acids Research 4673-4680 (1994); and iterative refinement,
see, e.g., Osamu Gotoh, Significant Improvement in Accuracy of
Multiple Protein Sequence Alignments by Iterative Refinement as
Assessed by Reference to Structural Alignments, 264(4) J. Mol.
Biol. 823-838 (1996).
[0041] Local methods align sequences by identifying one or more
conserved motifs shared by all of the input sequences. Non-limiting
methods include, e.g., Match-box, see, e.g., Eric Depiereux and
Ernest Feytmans, Match-Box: A Fundamentally New Algorithm for the
Simultaneous Alignment of Several Protein Sequences, 8(5) CABIOS
501-509 (1992); Gibbs sampling, see, e.g., C. E. Lawrence et al.,
Detecting Subtle Sequence Signals: A Gibbs Sampling Strategy for
Multiple Alignment, 262(5131) Science 208-214 (1993); Align-M, see,
e.g., Ivo Van Walle et al., Align-M--A New Algorithm for Multiple
Alignment of Highly Divergent Sequences, 20(9)
Bioinformatics:1428-1435 (2004).
[0042] Hybrid methods combine functional aspects of both global and
local alignment methods. Non-limiting methods include, e.g.,
segment-to-segment comparison, see, e.g., Burkhard Morgenstern et
al., Multiple DNA and Protein Sequence Alignment Based On
Segment-To-Segment Comparison, 93(22) Proc. Natl. Acad. Sci. U.S.A.
12098-12103 (1996); T-Coffee, see, e.g., Cedric Notredame et al.,
T-Coffee: A Novel Algorithm for Multiple Sequence Alignment, 302(1)
J. Mol. Biol. 205-217 (2000); MUSCLE, see, e.g., Robert C. Edgar,
MUSCLE: Multiple Sequence Alignment With High Score Accuracy and
High Throughput, 32(5) Nucleic Acids Res. 1792-1797 (2004); and
DIALIGN-T, see, e.g., Amarendran R Subramanian et al., DIALIGN-T:
An Improved Algorithm for Segment-Based Multiple Sequence
Alignment, 6(1) BMC Bioinformatics 66 (2005).
[0043] Thus, in an embodiment, a modified Clostridial toxin
disclosed in the present specification comprises a Clostridial
toxin enzymatic domain. In an aspect of this embodiment, a
Clostridial toxin enzymatic domain comprises a naturally occurring
Clostridial toxin light chain variant, such as, e.g., a Clostridial
toxin light chain isoform or a Clostridial toxin light chain
subtype. In another aspect of this embodiment, a Clostridial toxin
enzymatic domain comprises a non-naturally occurring Clostridial
toxin light chain variant, such as, e.g., a conservative
Clostridial toxin light chain variant, a non-conservative
Clostridial toxin light chain variant, a Clostridial toxin chimeric
light chain, an active Clostridial toxin light chain fragment, or
any combination thereof.
[0044] In another embodiment, a Clostridial toxin enzymatic domain
comprises a BoNT/A light chain. In an aspect of this embodiment, a
BoNT/A light chain comprises amino acids 1-448 of SEQ ID NO: 1. In
another aspect of this embodiment, a BoNT/A light chain comprises a
naturally occurring BoNT/A light chain variant, such as, e.g., a
light chain from a BoNT/A isoform or a light chain from a BoNT/A
subtype. In another aspect of this embodiment, a BoNT/A light chain
comprises amino acids 1-448 of a naturally occurring BoNT/A light
chain variant of SEQ ID NO: 1, such as, e.g., amino acids 1-448 of
a BoNT/A isoform of SEQ ID NO: 1 or amino acids 1-448 of a BoNT/A
subtype of SEQ ID NO: 1. In still another aspect of this
embodiment, a BoNT/A light chain comprises a non-naturally
occurring BoNT/A light chain variant, such as, e.g., a conservative
BoNT/A light chain variant, a non-conservative BoNT/A light chain
variant, a BoNT/A chimeric light chain, an active BoNT/A light
chain fragment, or any combination thereof. In still another aspect
of this embodiment, a BoNT/A light chain comprises amino acids
1-448 of a non-naturally occurring BoNT/A light chain variant of
SEQ ID NO: 1, such as, e.g., amino acids 1-448 of a conservative
BoNT/A light chain variant of SEQ ID NO: 1, amino acids 1-448 of a
non-conservative BoNT/A light chain variant of SEQ ID NO: 1, amino
acids 1-448 of an active BoNT/A light chain fragment of SEQ ID NO:
1, or any combination thereof.
[0045] In other aspects of this embodiment, a BoNT/A light chain
comprises a polypeptide having, e.g., at least 70% amino acid
identity with amino acids 1-448 of SEQ ID NO: 1, at least 75% amino
acid identity with amino acids 1-448 of SEQ ID NO: 1, at least 80%
amino acid identity with amino acids 1-448 of SEQ ID NO: 1, at
least 85% amino acid identity with amino acids 1-448 of SEQ ID NO:
1, at least 90% amino acid identity with amino acids 1-448 of SEQ
ID NO: 1 or at least 95% amino acid identity with amino acids 1-448
of SEQ ID NO: 1. In yet other aspects of this embodiment, a BoNT/A
light chain comprises a polypeptide having, e.g., at most 70% amino
acid identity with amino acids 1-448 of SEQ ID NO: 1, at most 75%
amino acid identity with amino acids 1-448 of SEQ ID NO: 1, at most
80% amino acid identity with amino acids 1-448 of SEQ ID NO: 1, at
most 85% amino acid identity with amino acids 1-448 of SEQ ID NO:
1, at most 90% amino acid identity with amino acids 1-448 of SEQ ID
NO: 1 or at most 95% amino acid identity with amino acids 1-448 of
SEQ ID NO: 1.
[0046] In other aspects of this embodiment, a BoNT/A light chain
comprises a polypeptide having, e.g., at most one, two, three,
four, five, six, seven, eight, nine, 10, 20, 30, 40, 50, 100, or
200 non-contiguous amino acid substitutions relative to amino acids
1-448 of SEQ ID NO: 1. In other aspects of this embodiment, a
BoNT/A light chain comprises a polypeptide having, e.g., at least
one, two, three, four, five, six, seven, eight, nine, 10, 20, 30,
40, 50, 100 or 200 non-contiguous amino acid substitutions relative
to amino acids 1-448 of SEQ ID NO: 1. In yet other aspects of this
embodiment, a BoNT/A light chain comprises a polypeptide having,
e.g., at most one, two, three, four, five, six, seven, eight, nine,
10, 20, 30, 40, 50, 100 or 200 non-contiguous amino acid deletions
relative to amino acids 1-448 of SEQ ID NO: 1. In other aspects of
this embodiment, a BoNT/A light chain comprises a polypeptide
having, e.g., at least one, two, three, four, five, six, seven,
eight, nine, 10, 20, 30, 40, 50, 100 or 200 non-contiguous amino
acid deletions relative to amino acids 1-448 of SEQ ID NO: 1. In
still other aspects of this embodiment, a BoNT/A light chain
comprises a polypeptide having, e.g., at most one, two, three,
four, five, six, seven, eight, nine, 10, 20, 30, 40, 50, 100 or 200
non-contiguous amino acid additions relative to amino acids 1-448
of SEQ ID NO: 1. In other aspects of this embodiment, a BoNT/A
light chain comprises a polypeptide having, e.g., at least one,
two, three, four, five, six, seven, eight, nine, 10, 20, 30, 40,
50, 100 or 200 non-contiguous amino acid additions relative to
amino acids 1-448 of SEQ ID NO: 1.
[0047] In other aspects of this embodiment, a BoNT/A light chain
comprises a polypeptide having, e.g., at most one, two, three,
four, five, six, seven, eight, nine, 10, 20, 30, 40, 50, 100 or 200
contiguous amino acid substitutions relative to amino acids 1-448
of SEQ ID NO: 1. In other aspects of this embodiment, a BoNT/A
light chain comprises a polypeptide having, e.g., at least one,
two, three, four, five, six, seven, eight, nine, 10, 20, 30, 40,
50, 100 or 200 contiguous amino acid substitutions relative to
amino acids 1-448 of SEQ ID NO: 1. In yet other aspects of this
embodiment, a BoNT/A light chain comprises a polypeptide having,
e.g., at most one, two, three, four, five, six, seven, eight, nine,
10, 20, 30, 40, 50, 100 or 200 contiguous amino acid deletions
relative to amino acids 1-448 of SEQ ID NO: 1. In other aspects of
this embodiment, a BoNT/A light chain comprises a polypeptide
having, e.g., at least one, two, three, four, five, six, seven,
eight, nine, 10, 20, 30, 40, 50, 100 or 200 contiguous amino acid
deletions relative to amino acids 1-448 of SEQ ID NO: 1. In still
other aspects of this embodiment, a BoNT/A light chain comprises a
polypeptide having, e.g., at most one, two, three, four, five, six,
seven, eight, nine, 10, 20, 30, 40, 50, 100 or 200 contiguous amino
acid additions relative to amino acids 1-448 of SEQ ID NO: 1. In
other aspects of this embodiment, a BoNT/A light chain comprises a
polypeptide having, e.g., at least one, two, three, four, five,
six, seven, eight, nine, 10, 20, 30, 40, 50, 100 or 200 contiguous
amino acid additions relative to amino acids 1-448 of SEQ ID NO:
1.
[0048] In another embodiment, a Clostridial toxin enzymatic domain
comprises a BoNT/B light chain. In an aspect of this embodiment, a
BoNT/B light chain comprises amino acids 1-441 of SEQ ID NO: 2. In
another aspect of this embodiment, a BoNT/B light chain comprises a
naturally occurring BoNT/B light chain variant, such as, e.g., a
light chain from a BoNT/B isoform or a light chain from a BoNT/B
subtype. In another aspect of this embodiment, a BoNT/B light chain
comprises amino acids 1-441 of a naturally occurring BoNT/B light
chain variant of SEQ ID NO: 2, such as, e.g., amino acids 1-441 of
a BoNT/B isoform of SEQ ID NO: 2 or amino acids 1-441 of a BoNT/B
subtype of SEQ ID NO: 2. In still another aspect of this
embodiment, a BoNT/B light chain comprises a non-naturally
occurring BoNT/B light chain variant, such as, e.g., a conservative
BoNT/B light chain variant, a non-conservative BoNT/B light chain
variant, a BoNT/B chimeric light chain, an active BoNT/B light
chain fragment, or any combination thereof. In still another aspect
of this embodiment, a BoNT/B light chain comprises amino acids
1-441 of a non-naturally occurring BoNT/B light chain variant of
SEQ ID NO: 2, such as, e.g., amino acids 1-441 of a conservative
BoNT/B light chain variant of SEQ ID NO: 2, amino acids 1-441 of a
non-conservative BoNT/B light chain variant of SEQ ID NO: 2, amino
acids 1-441 of an active BoNT/B light chain fragment of SEQ ID NO:
2, or any combination thereof.
[0049] In other aspects of this embodiment, a BoNT/B light chain
comprises a polypeptide having, e.g., at least 70% amino acid
identity with amino acids 1-441 of SEQ ID NO: 2, at least 75% amino
acid identity with amino acids 1-441 of SEQ ID NO: 2, at least 80%
amino acid identity with amino acids 1-441 of SEQ ID NO: 2, at
least 85% amino acid identity with amino acids 1-441 of SEQ ID NO:
2, at least 90% amino acid identity with amino acids 1-441 of SEQ
ID NO: 2 or at least 95% amino acid identity with amino acids 1-441
of SEQ ID NO: 2. In yet other aspects of this embodiment, a BoNT/B
light chain comprises a polypeptide having, e.g., at most 70% amino
acid identity with amino acids 1-441 of SEQ ID NO: 2, at most 75%
amino acid identity with amino acids 1-441 of SEQ ID NO: 2, at most
80% amino acid identity with amino acids 1-441 of SEQ ID NO: 2, at
most 85% amino acid identity with amino acids 1-441 of SEQ ID NO:
2, at most 90% amino acid identity with amino acids 1-441 of SEQ ID
NO: 2 or at most 95% amino acid identity with amino acids 1-441 of
SEQ ID NO: 2.
[0050] In other aspects of this embodiment, a BoNT/B light chain
comprises a polypeptide having, e.g., at most one, two, three,
four, five, six, seven, eight, nine, 10, 20, 30, 40, 50, 100, or
200 non-contiguous amino acid substitutions relative to amino acids
1-441 of SEQ ID NO: 2. In other aspects of this embodiment, a
BoNT/B light chain comprises a polypeptide having, e.g., at least
one, two, three, four, five, six, seven, eight, nine, 10, 20, 30,
40, 50, 100 or 200 non-contiguous amino acid substitutions relative
to amino acids 1-441 of SEQ ID NO: 2. In yet other aspects of this
embodiment, a BoNT/B light chain comprises a polypeptide having,
e.g., at most one, two, three, four, five, six, seven, eight, nine,
10, 20, 30, 40, 50, 100 or 200 non-contiguous amino acid deletions
relative to amino acids 1-441 of SEQ ID NO: 2. In other aspects of
this embodiment, a BoNT/B light chain comprises a polypeptide
having, e.g., at least one, two, three, four, five, six, seven,
eight, nine, 10, 20, 30, 40, 50, 100 or 200 non-contiguous amino
acid deletions relative to amino acids 1-441 of SEQ ID NO: 2. In
still other aspects of this embodiment, a BoNT/B light chain
comprises a polypeptide having, e.g., at most one, two, three,
four, five, six, seven, eight, nine, 10, 20, 30, 40, 50, 100 or 200
non-contiguous amino acid additions relative to amino acids 1-441
of SEQ ID NO: 2. In other aspects of this embodiment, a BoNT/B
light chain comprises a polypeptide having, e.g., at least one,
two, three, four, five, six, seven, eight, nine, 10, 20, 30, 40,
50, 100 or 200 non-contiguous amino acid additions relative to
amino acids 1-441 of SEQ ID NO: 2.
[0051] In other aspects of this embodiment, a BoNT/B light chain
comprises a polypeptide having, e.g., at most one, two, three,
four, five, six, seven, eight, nine, 10, 20, 30, 40, 50, 100 or 200
contiguous amino acid substitutions relative to amino acids 1-441
of SEQ ID NO: 2. In other aspects of this embodiment, a BoNT/B
light chain comprises a polypeptide having, e.g., at least one,
two, three, four, five, six, seven, eight, nine, 10, 20, 30, 40,
50, 100 or 200 contiguous amino acid substitutions relative to
amino acids 1-441 of SEQ ID NO: 2. In yet other aspects of this
embodiment, a BoNT/B light chain comprises a polypeptide having,
e.g., at most one, two, three, four, five, six, seven, eight, nine,
10, 20, 30, 40, 50, 100 or 200 contiguous amino acid deletions
relative to amino acids 1-441 of SEQ ID NO: 2. In other aspects of
this embodiment, a BoNT/B light chain comprises a polypeptide
having, e.g., at least one, two, three, four, five, six, seven,
eight, nine, 10, 20, 30, 40, 50, 100 or 200 contiguous amino acid
deletions relative to amino acids 1-441 of SEQ ID NO: 2. In still
other aspects of this embodiment, a BoNT/B light chain comprises a
polypeptide having, e.g., at most one, two, three, four, five, six,
seven, eight, nine, 10, 20, 30, 40, 50, 100 or 200 contiguous amino
acid additions relative to amino acids 1-441 of SEQ ID NO: 2. In
other aspects of this embodiment, a BoNT/B light chain comprises a
polypeptide having, e.g., at least one, two, three, four, five,
six, seven, eight, nine, 10, 20, 30, 40, 50, 100 or 200 contiguous
amino acid additions relative to amino acids 1-441 of SEQ ID NO:
2.
[0052] In another embodiment, a Clostridial toxin enzymatic domain
comprises a BoNT/C1 light chain. In an aspect of this embodiment, a
BoNT/C1 light chain comprises amino acids 1-449 of SEQ ID NO: 3. In
another aspect of this embodiment, a BoNT/C1 light chain comprises
a naturally occurring BoNT/C1 light chain variant, such as, e.g., a
light chain from a BoNT/C1 isoform or a light chain from a BoNT/C1
subtype. In another aspect of this embodiment, a BoNT/C1 light
chain comprises amino acids 1-449 of a naturally occurring BoNT/C1
light chain variant of SEQ ID NO: 3, such as, e.g., amino acids
1-449 of a BoNT/C1 isoform of SEQ ID NO: 3 or amino acids 1-449 of
a BoNT/C1 subtype of SEQ ID NO: 3. In still another aspect of this
embodiment, a BoNT/C1 light chain comprises a non-naturally
occurring BoNT/C1 light chain variant, such as, e.g., a
conservative BoNT/C1 light chain variant, a non-conservative
BoNT/C1 light chain variant, a BoNT/C1 chimeric light chain, an
active BoNT/C1 light chain fragment, or any combination thereof. In
still another aspect of this embodiment, a BoNT/C1 light chain
comprises amino acids 1-449 of a non-naturally occurring BoNT/C1
light chain variant of SEQ ID NO: 3, such as, e.g., amino acids
1-449 of a conservative BoNT/C1 light chain variant of SEQ ID NO:
3, amino acids 1-449 of a non-conservative BoNT/C1 light chain
variant of SEQ ID NO: 3, amino acids 1-449 of an active BoNT/C1
light chain fragment of SEQ ID NO: 3, or any combination
thereof.
[0053] In other aspects of this embodiment, a BoNT/C1 light chain
comprises a polypeptide having, e.g., at least 70% amino acid
identity with amino acids 1-449 of SEQ ID NO: 3, at least 75% amino
acid identity with amino acids 1-449 of SEQ ID NO: 3, at least 80%
amino acid identity with amino acids 1-449 of SEQ ID NO: 3, at
least 85% amino acid identity with amino acids 1-449 of SEQ ID NO:
3, at least 90% amino acid identity with amino acids 1-449 of SEQ
ID NO: 3 or at least 95% amino acid identity with amino acids 1-449
of SEQ ID NO: 3. In yet other aspects of this embodiment, a BoNT/C1
light chain comprises a polypeptide having, e.g., at most 70% amino
acid identity with amino acids 1-449 of SEQ ID NO: 3, at most 75%
amino acid identity with amino acids 1-449 of SEQ ID NO: 3, at most
80% amino acid identity with amino acids 1-449 of SEQ ID NO: 3, at
most 85% amino acid identity with amino acids 1-449 of SEQ ID NO:
3, at most 90% amino acid identity with amino acids 1-449 of SEQ ID
NO: 3 or at most 95% amino acid identity with amino acids 1-449 of
SEQ ID NO: 3.
[0054] In other aspects of this embodiment, a BoNT/C1 light chain
comprises a polypeptide having, e.g., at most one, two, three,
four, five, six, seven, eight, nine, 10, 20, 30, 40, 50, 100, or
200 non-contiguous amino acid substitutions relative to amino acids
1-449 of SEQ ID NO: 3. In other aspects of this embodiment, a
BoNT/C1 light chain comprises a polypeptide having, e.g., at least
one, two, three, four, five, six, seven, eight, nine, 10, 20, 30,
40, 50, 100 or 200 non-contiguous amino acid substitutions relative
to amino acids 1-449 of SEQ ID NO: 3. In yet other aspects of this
embodiment, a BoNT/C1 light chain comprises a polypeptide having,
e.g., at most one, two, three, four, five, six, seven, eight, nine,
10, 20, 30, 40, 50, 100 or 200 non-contiguous amino acid deletions
relative to amino acids 1-449 of SEQ ID NO: 3. In other aspects of
this embodiment, a BoNT/C1 light chain comprises a polypeptide
having, e.g., at least one, two, three, four, five, six, seven,
eight, nine, 10, 20, 30, 40, 50, 100 or 200 non-contiguous amino
acid deletions relative to amino acids 1-449 of SEQ ID NO: 3. In
still other aspects of this embodiment, a BoNT/C1 light chain
comprises a polypeptide having, e.g., at most one, two, three,
four, five, six, seven, eight, nine, 10, 20, 30, 40, 50, 100 or 200
non-contiguous amino acid additions relative to amino acids 1-449
of SEQ ID NO: 3. In other aspects of this embodiment, a BoNT/C1
light chain comprises a polypeptide having, e.g., at least one,
two, three, four, five, six, seven, eight, nine, 10, 20, 30, 40,
50, 100 or 200 non-contiguous amino acid additions relative to
amino acids 1-449 of SEQ ID NO: 3.
[0055] In other aspects of this embodiment, a BoNT/C1 light chain
comprises a polypeptide having, e.g., at most one, two, three,
four, five, six, seven, eight, nine, 10, 20, 30, 40, 50, 100 or 200
contiguous amino acid substitutions relative to amino acids 1-449
of SEQ ID NO: 3. In other aspects of this embodiment, a BoNT/C1
light chain comprises a polypeptide having, e.g., at least one,
two, three, four, five, six, seven, eight, nine, 10, 20, 30, 40,
50, 100 or 200 contiguous amino acid substitutions relative to
amino acids 1-449 of SEQ ID NO: 3. In yet other aspects of this
embodiment, a BoNT/C1 light chain comprises a polypeptide having,
e.g., at most one, two, three, four, five, six, seven, eight, nine,
10, 20, 30, 40, 50, 100 or 200 contiguous amino acid deletions
relative to amino acids 1-449 of SEQ ID NO: 3. In other aspects of
this embodiment, a BoNT/C1 light chain comprises a polypeptide
having, e.g., at least one, two, three, four, five, six, seven,
eight, nine, 10, 20, 30, 40, 50, 100 or 200 contiguous amino acid
deletions relative to amino acids 1-449 of SEQ ID NO: 3. In still
other aspects of this embodiment, a BoNT/C1 light chain comprises a
polypeptide having, e.g., at most one, two, three, four, five, six,
seven, eight, nine, 10, 20, 30, 40, 50, 100 or 200 contiguous amino
acid additions relative to amino acids 1-449 of SEQ ID NO: 3. In
other aspects of this embodiment, a BoNT/C1 light chain comprises a
polypeptide having, e.g., at least one, two, three, four, five,
six, seven, eight, nine, 10, 20, 30, 40, 50, 100 or 200 contiguous
amino acid additions relative to amino acids 1-449 of SEQ ID NO:
3.
[0056] In another embodiment, a Clostridial toxin enzymatic domain
comprises a BoNT/D light chain. In an aspect of this embodiment, a
BoNT/D light chain comprises amino acids 1-445 of SEQ ID NO: 4. In
another aspect of this embodiment, a BoNT/D light chain comprises a
naturally occurring BoNT/D light chain variant, such as, e.g., a
light chain from a BoNT/D isoform or a light chain from a BoNT/D
subtype. In another aspect of this embodiment, a BoNT/D light chain
comprises amino acids 1-445 of a naturally occurring BoNT/D light
chain variant of SEQ ID NO: 4, such as, e.g., amino acids 1-445 of
a BoNT/D isoform of SEQ ID NO: 4 or amino acids 1-445 of a BoNT/D
subtype of SEQ ID NO: 4. In still another aspect of this
embodiment, a BoNT/D light chain comprises a non-naturally
occurring BoNT/D light chain variant, such as, e.g., a conservative
BoNT/D light chain variant, a non-conservative BoNT/D light chain
variant, a BoNT/D chimeric light chain, an active BoNT/D light
chain fragment, or any combination thereof. In still another aspect
of this embodiment, a BoNT/D light chain comprises amino acids
1-445 of a non-naturally occurring BoNT/D light chain variant of
SEQ ID NO: 4, such as, e.g., amino acids 1-445 of a conservative
BoNT/D light chain variant of SEQ ID NO: 4, amino acids 1-445 of a
non-conservative BoNT/D light chain variant of SEQ ID NO: 4, amino
acids 1-445 of an active BoNT/D light chain fragment of SEQ ID NO:
4, or any combination thereof.
[0057] In other aspects of this embodiment, a BoNT/D light chain
comprises a polypeptide having, e.g., at least 70% amino acid
identity with amino acids 1-445 of SEQ ID NO: 4, at least 75% amino
acid identity with amino acids 1-445 of SEQ ID NO: 4, at least 80%
amino acid identity with amino acids 1-445 of SEQ ID NO: 4, at
least 85% amino acid identity with amino acids 1-445 of SEQ ID NO:
4, at least 90% amino acid identity with amino acids 1-445 of SEQ
ID NO: 4 or at least 95% amino acid identity with amino acids 1-445
of SEQ ID NO: 4. In yet other aspects of this embodiment, a BoNT/D
light chain comprises a polypeptide having, e.g., at most 70% amino
acid identity with amino acids 1-445 of SEQ ID NO: 4, at most 75%
amino acid identity with amino acids 1-445 of SEQ ID NO: 4, at most
80% amino acid identity with amino acids 1-445 of SEQ ID NO: 4, at
most 85% amino acid identity with amino acids 1-445 of SEQ ID NO:
4, at most 90% amino acid identity with amino acids 1-445 of SEQ ID
NO: 4 or at most 95% amino acid identity with amino acids 1-445 of
SEQ ID NO: 4.
[0058] In other aspects of this embodiment, a BoNT/D light chain
comprises a polypeptide having, e.g., at most one, two, three,
four, five, six, seven, eight, nine, 10, 20, 30, 40, 50, 100, or
200 non-contiguous amino acid substitutions relative to amino acids
1-445 of SEQ ID NO: 4. In other aspects of this embodiment, a
BoNT/D light chain comprises a polypeptide having, e.g., at least
one, two, three, four, five, six, seven, eight, nine, 10, 20, 30,
40, 50, 100 or 200 non-contiguous amino acid substitutions relative
to amino acids 1-445 of SEQ ID NO: 4. In yet other aspects of this
embodiment, a BoNT/D light chain comprises a polypeptide having,
e.g., at most one, two, three, four, five, six, seven, eight, nine,
10, 20, 30, 40, 50, 100 or 200 non-contiguous amino acid deletions
relative to amino acids 1-445 of SEQ ID NO: 4. In other aspects of
this embodiment, a BoNT/D light chain comprises a polypeptide
having, e.g., at least one, two, three, four, five, six, seven,
eight, nine, 10, 20, 30, 40, 50, 100 or 200 non-contiguous amino
acid deletions relative to amino acids 1-445 of SEQ ID NO: 4. In
still other aspects of this embodiment, a BoNT/D light chain
comprises a polypeptide having, e.g., at most one, two, three,
four, five, six, seven, eight, nine, 10, 20, 30, 40, 50, 100 or 200
non-contiguous amino acid additions relative to amino acids 1-445
of SEQ ID NO: 4. In other aspects of this embodiment, a BoNT/D
light chain comprises a polypeptide having, e.g., at least one,
two, three, four, five, six, seven, eight, nine, 10, 20, 30, 40,
50, 100 or 200 non-contiguous amino acid additions relative to
amino acids 1-445 of SEQ ID NO: 4.
[0059] In other aspects of this embodiment, a BoNT/D light chain
comprises a polypeptide having, e.g., at most one, two, three,
four, five, six, seven, eight, nine, 10, 20, 30, 40, 50, 100 or 200
contiguous amino acid substitutions relative to amino acids 1-445
of SEQ ID NO: 4. In other aspects of this embodiment, a BoNT/D
light chain comprises a polypeptide having, e.g., at least one,
two, three, four, five, six, seven, eight, nine, 10, 20, 30, 40,
50, 100 or 200 contiguous amino acid substitutions relative to
amino acids 1-445 of SEQ ID NO: 4. In yet other aspects of this
embodiment, a BoNT/D light chain comprises a polypeptide having,
e.g., at most one, two, three, four, five, six, seven, eight, nine,
10, 20, 30, 40, 50, 100 or 200 contiguous amino acid deletions
relative to amino acids 1-445 of SEQ ID NO: 4. In other aspects of
this embodiment, a BoNT/D light chain comprises a polypeptide
having, e.g., at least one, two, three, four, five, six, seven,
eight, nine, 10, 20, 30, 40, 50, 100 or 200 contiguous amino acid
deletions relative to amino acids 1-445 of SEQ ID NO: 4. In still
other aspects of this embodiment, a BoNT/D light chain comprises a
polypeptide having, e.g., at most one, two, three, four, five, six,
seven, eight, nine, 10, 20, 30, 40, 50, 100 or 200 contiguous amino
acid additions relative to amino acids 1-445 of SEQ ID NO: 4. In
other aspects of this embodiment, a BoNT/D light chain comprises a
polypeptide having, e.g., at least one, two, three, four, five,
six, seven, eight, nine, 10, 20, 30, 40, 50, 100 or 200 contiguous
amino acid additions relative to amino acids 1-445 of SEQ ID NO:
4.
[0060] In another embodiment, a Clostridial toxin enzymatic domain
comprises a BoNT/E light chain. In an aspect of this embodiment, a
BoNT/E light chain comprises amino acids 1-422 of SEQ ID NO: 5. In
another aspect of this embodiment, a BoNT/E light chain comprises a
naturally occurring BoNT/E light chain variant, such as, e.g., a
light chain from a BoNT/E isoform or a light chain from a BoNT/E
subtype. In another aspect of this embodiment, a BoNT/E light chain
comprises amino acids 1-422 of a naturally occurring BoNT/E light
chain variant of SEQ ID NO: 5, such as, e.g., amino acids 1-422 of
a BoNT/E isoform of SEQ ID NO: 5 or amino acids 1-422 of a BoNT/E
subtype of SEQ ID NO: 5. In still another aspect of this
embodiment, a BoNT/E light chain comprises a non-naturally
occurring BoNT/E light chain variant, such as, e.g., a conservative
BoNT/E light chain variant, a non-conservative BoNT/E light chain
variant, a BoNT/E chimeric light chain, an active BoNT/E light
chain fragment, or any combination thereof. In still another aspect
of this embodiment, a BoNT/E light chain comprises amino acids
1-422 of a non-naturally occurring BoNT/E light chain variant of
SEQ ID NO: 5, such as, e.g., amino acids 1-422 of a conservative
BoNT/E light chain variant of SEQ ID NO: 5, amino acids 1-422 of a
non-conservative BoNT/E light chain variant of SEQ ID NO: 5, amino
acids 1-422 of an active BoNT/E light chain fragment of SEQ ID NO:
5, or any combination thereof.
[0061] In other aspects of this embodiment, a BoNT/E light chain
comprises a polypeptide having, e.g., at least 70% amino acid
identity with amino acids 1-422 of SEQ ID NO: 5, at least 75% amino
acid identity with amino acids 1-422 of SEQ ID NO: 5, at least 80%
amino acid identity with amino acids 1-422 of SEQ ID NO: 5, at
least 85% amino acid identity with amino acids 1-422 of SEQ ID NO:
5, at least 90% amino acid identity with amino acids 1-422 of SEQ
ID NO: 5 or at least 95% amino acid identity with amino acids 1-422
of SEQ ID NO: 5. In yet other aspects of this embodiment, a BoNT/E
light chain comprises a polypeptide having, e.g., at most 70% amino
acid identity with amino acids 1-422 of SEQ ID NO: 5, at most 75%
amino acid identity with amino acids 1-422 of SEQ ID NO: 5, at most
80% amino acid identity with amino acids 1-422 of SEQ ID NO: 5, at
most 85% amino acid identity with amino acids 1-422 of SEQ ID NO:
5, at most 90% amino acid identity with amino acids 1-422 of SEQ ID
NO: 5 or at most 95% amino acid identity with amino acids 1-422 of
SEQ ID NO: 5.
[0062] In other aspects of this embodiment, a BoNT/E light chain
comprises a polypeptide having, e.g., at most one, two, three,
four, five, six, seven, eight, nine, 10, 20, 30, 40, 50, 100, or
200 non-contiguous amino acid substitutions relative to amino acids
1-422 of SEQ ID NO: 5. In other aspects of this embodiment, a
BoNT/E light chain comprises a polypeptide having, e.g., at least
one, two, three, four, five, six, seven, eight, nine, 10, 20, 30,
40, 50, 100 or 200 non-contiguous amino acid substitutions relative
to amino acids 1-422 of SEQ ID NO: 5. In yet other aspects of this
embodiment, a BoNT/E light chain comprises a polypeptide having,
e.g., at most one, two, three, four, five, six, seven, eight, nine,
10, 20, 30, 40, 50, 100 or 200 non-contiguous amino acid deletions
relative to amino acids 1-422 of SEQ ID NO: 5. In other aspects of
this embodiment, a BoNT/E light chain comprises a polypeptide
having, e.g., at least one, two, three, four, five, six, seven,
eight, nine, 10, 20, 30, 40, 50, 100 or 200 non-contiguous amino
acid deletions relative to amino acids 1-422 of SEQ ID NO: 5. In
still other aspects of this embodiment, a BoNT/E light chain
comprises a polypeptide having, e.g., at most one, two, three,
four, five, six, seven, eight, nine, 10, 20, 30, 40, 50, 100 or 200
non-contiguous amino acid additions relative to amino acids 1-422
of SEQ ID NO: 5. In other aspects of this embodiment, a BoNT/E
light chain comprises a polypeptide having, e.g., at least one,
two, three, four, five, six, seven, eight, nine, 10, 20, 30, 40,
50, 100 or 200 non-contiguous amino acid additions relative to
amino acids 1-422 of SEQ ID NO: 5.
[0063] In other aspects of this embodiment, a BoNT/E light chain
comprises a polypeptide having, e.g., at most one, two, three,
four, five, six, seven, eight, nine, 10, 20, 30, 40, 50, 100 or 200
contiguous amino acid substitutions relative to amino acids 1-422
of SEQ ID NO: 5. In other aspects of this embodiment, a BoNT/E
light chain comprises a polypeptide having, e.g., at least one,
two, three, four, five, six, seven, eight, nine, 10, 20, 30, 40,
50, 100 or 200 contiguous amino acid substitutions relative to
amino acids 1-422 of SEQ ID NO: 5. In yet other aspects of this
embodiment, a BoNT/E light chain comprises a polypeptide having,
e.g., at most one, two, three, four, five, six, seven, eight, nine,
10, 20, 30, 40, 50, 100 or 200 contiguous amino acid deletions
relative to amino acids 1-422 of SEQ ID NO: 5. In other aspects of
this embodiment, a BoNT/E light chain comprises a polypeptide
having, e.g., at least one, two, three, four, five, six, seven,
eight, nine, 10, 20, 30, 40, 50, 100 or 200 contiguous amino acid
deletions relative to amino acids 1-422 of SEQ ID NO: 5. In still
other aspects of this embodiment, a BoNT/E light chain comprises a
polypeptide having, e.g., at most one, two, three, four, five, six,
seven, eight, nine, 10, 20, 30, 40, 50, 100 or 200 contiguous amino
acid additions relative to amino acids 1-422 of SEQ ID NO: 5. In
other aspects of this embodiment, a BoNT/E light chain comprises a
polypeptide having, e.g., at least one, two, three, four, five,
six, seven, eight, nine, 10, 20, 30, 40, 50, 100 or 200 contiguous
amino acid additions relative to amino acids 1-422 of SEQ ID NO:
5.
[0064] In another embodiment, a Clostridial toxin enzymatic domain
comprises a BoNT/F light chain. In an aspect of this embodiment, a
BoNT/F light chain comprises amino acids 1-439 of SEQ ID NO: 6. In
another aspect of this embodiment, a BoNT/F light chain comprises a
naturally occurring BoNT/F light chain variant, such as, e.g., a
light chain from a BoNT/F isoform or a light chain from a BoNT/F
subtype. In another aspect of this embodiment, a BoNT/F light chain
comprises amino acids 1-439 of a naturally occurring BoNT/F light
chain variant of SEQ ID NO: 6, such as, e.g., amino acids 1-439 of
a BoNT/F isoform of SEQ ID NO: 6 or amino acids 1-439 of a BoNT/F
subtype of SEQ ID NO: 6. In still another aspect of this
embodiment, a BoNT/F light chain comprises a non-naturally
occurring BoNT/F light chain variant, such as, e.g., a conservative
BoNT/F light chain variant, a non-conservative BoNT/F light chain
variant, a BoNT/F chimeric light chain, an active BoNT/F light
chain fragment, or any combination thereof. In still another aspect
of this embodiment, a BoNT/F light chain comprises amino acids
1-439 of a non-naturally occurring BoNT/F light chain variant of
SEQ ID NO: 6, such as, e.g., amino acids 1-439 of a conservative
BoNT/F light chain variant of SEQ ID NO: 6, amino acids 1-439 of a
non-conservative BoNT/F light chain variant of SEQ ID NO: 6, amino
acids 1-439 of an active BoNT/F light chain fragment of SEQ ID NO:
6, or any combination thereof.
[0065] In other aspects of this embodiment, a BoNT/F light chain
comprises a polypeptide having, e.g., at least 70% amino acid
identity with amino acids 1-439 of SEQ ID NO: 6, at least 75% amino
acid identity with amino acids 1-439 of SEQ ID NO: 6, at least 80%
amino acid identity with amino acids 1-439 of SEQ ID NO: 6, at
least 85% amino acid identity with amino acids 1-439 of SEQ ID NO:
6, at least 90% amino acid identity with amino acids 1-439 of SEQ
ID NO: 6 or at least 95% amino acid identity with amino acids 1-439
of SEQ ID NO: 6. In yet other aspects of this embodiment, a BoNT/F
light chain comprises a polypeptide having, e.g., at most 70% amino
acid identity with amino acids 1-439 of SEQ ID NO: 6, at most 75%
amino acid identity with amino acids 1-439 of SEQ ID NO: 6, at most
80% amino acid identity with amino acids 1-439 of SEQ ID NO: 6, at
most 85% amino acid identity with amino acids 1-439 of SEQ ID NO:
6, at most 90% amino acid identity with amino acids 1-439 of SEQ ID
NO: 6 or at most 95% amino acid identity with amino acids 1-439 of
SEQ ID NO: 6.
[0066] In other aspects of this embodiment, a BoNT/F light chain
comprises a polypeptide having, e.g., at most one, two, three,
four, five, six, seven, eight, nine, 10, 20, 30, 40, 50, 100, or
200 non-contiguous amino acid substitutions relative to amino acids
1-439 of SEQ ID NO: 6. In other aspects of this embodiment, a
BoNT/F light chain comprises a polypeptide having, e.g., at least
one, two, three, four, five, six, seven, eight, nine, 10, 20, 30,
40, 50, 100 or 200 non-contiguous amino acid substitutions relative
to amino acids 1-439 of SEQ ID NO: 6. In yet other aspects of this
embodiment, a BoNT/F light chain comprises a polypeptide having,
e.g., at most one, two, three, four, five, six, seven, eight, nine,
10, 20, 30, 40, 50, 100 or 200 non-contiguous amino acid deletions
relative to amino acids 1-439 of SEQ ID NO: 6. In other aspects of
this embodiment, a BoNT/F light chain comprises a polypeptide
having, e.g., at least one, two, three, four, five, six, seven,
eight, nine, 10, 20, 30, 40, 50, 100 or 200 non-contiguous amino
acid deletions relative to amino acids 1-439 of SEQ ID NO: 6. In
still other aspects of this embodiment, a BoNT/F light chain
comprises a polypeptide having, e.g., at most one, two, three,
four, five, six, seven, eight, nine, 10, 20, 30, 40, 50, 100 or 200
non-contiguous amino acid additions relative to amino acids 1-439
of SEQ ID NO: 6. In other aspects of this embodiment, a BoNT/F
light chain comprises a polypeptide having, e.g., at least one,
two, three, four, five, six, seven, eight, nine, 10, 20, 30, 40,
50, 100 or 200 non-contiguous amino acid additions relative to
amino acids 1-439 of SEQ ID NO: 6.
[0067] In other aspects of this embodiment, a BoNT/F light chain
comprises a polypeptide having, e.g., at most one, two, three,
four, five, six, seven, eight, nine, 10, 20, 30, 40, 50, 100 or 200
contiguous amino acid substitutions relative to amino acids 1-439
of SEQ ID NO: 6. In other aspects of this embodiment, a BoNT/F
light chain comprises a polypeptide having, e.g., at least one,
two, three, four, five, six, seven, eight, nine, 10, 20, 30, 40,
50, 100 or 200 contiguous amino acid substitutions relative to
amino acids 1-439 of SEQ ID NO: 6. In yet other aspects of this
embodiment, a BoNT/F light chain comprises a polypeptide having,
e.g., at most one, two, three, four, five, six, seven, eight, nine,
10, 20, 30, 40, 50, 100 or 200 contiguous amino acid deletions
relative to amino acids 1-439 of SEQ ID NO: 6. In other aspects of
this embodiment, a BoNT/F light chain comprises a polypeptide
having, e.g., at least one, two, three, four, five, six, seven,
eight, nine, 10, 20, 30, 40, 50, 100 or 200 contiguous amino acid
deletions relative to amino acids 1-439 of SEQ ID NO: 6. In still
other aspects of this embodiment, a BoNT/F light chain comprises a
polypeptide having, e.g., at most one, two, three, four, five, six,
seven, eight, nine, 10, 20, 30, 40, 50, 100 or 200 contiguous amino
acid additions relative to amino acids 1-439 of SEQ ID NO: 6. In
other aspects of this embodiment, a BoNT/F light chain comprises a
polypeptide having, e.g., at least one, two, three, four, five,
six, seven, eight, nine, 10, 20, 30, 40, 50, 100 or 200 contiguous
amino acid additions relative to amino acids 1-439 of SEQ ID NO:
6.
[0068] In another embodiment, a Clostridial toxin enzymatic domain
comprises a BoNT/G light chain. In an aspect of this embodiment, a
BoNT/G light chain comprises amino acids 1-446 of SEQ ID NO: 7. In
another aspect of this embodiment, a BoNT/G light chain comprises a
naturally occurring BoNT/G light chain variant, such as, e.g., a
light chain from a BoNT/G isoform or a light chain from a BoNT/G
subtype. In another aspect of this embodiment, a BoNT/G light chain
comprises amino acids 1-446 of a naturally occurring BoNT/G light
chain variant of SEQ ID NO: 7, such as, e.g., amino acids 1-446 of
a BoNT/G isoform of SEQ ID NO: 7 or amino acids 1-446 of a BoNT/G
subtype of SEQ ID NO: 7. In still another aspect of this
embodiment, a BoNT/G light chain comprises a non-naturally
occurring BoNT/G light chain variant, such as, e.g., a conservative
BoNT/G light chain variant, a non-conservative BoNT/G light chain
variant, a BoNT/G chimeric light chain, an active BoNT/G light
chain fragment, or any combination thereof. In still another aspect
of this embodiment, a BoNT/G light chain comprises amino acids
1-446 of a non-naturally occurring BoNT/G light chain variant of
SEQ ID NO: 7, such as, e.g., amino acids 1-446 of a conservative
BoNT/G light chain variant of SEQ ID NO: 7, amino acids 1-446 of a
non-conservative BoNT/G light chain variant of SEQ ID NO: 7, amino
acids 1-446 of an active BoNT/G light chain fragment of SEQ ID NO:
7, or any combination thereof.
[0069] In other aspects of this embodiment, a BoNT/G light chain
comprises a polypeptide having, e.g., at least 70% amino acid
identity with amino acids 1-446 of SEQ ID NO: 7, at least 75% amino
acid identity with amino acids 1-446 of SEQ ID NO: 7, at least 80%
amino acid identity with amino acids 1-446 of SEQ ID NO: 7, at
least 85% amino acid identity with amino acids 1-446 of SEQ ID NO:
7, at least 90% amino acid identity with amino acids 1-446 of SEQ
ID NO: 7 or at least 95% amino acid identity with amino acids 1-446
of SEQ ID NO: 7. In yet other aspects of this embodiment, a BoNT/G
light chain comprises a polypeptide having, e.g., at most 70% amino
acid identity with amino acids 1-446 of SEQ ID NO: 7, at most 75%
amino acid identity with amino acids 1-446 of SEQ ID NO: 7, at most
80% amino acid identity with amino acids 1-446 of SEQ ID NO: 7, at
most 85% amino acid identity with amino acids 1-446 of SEQ ID NO:
7, at most 90% amino acid identity with amino acids 1-446 of SEQ ID
NO: 7 or at most 95% amino acid identity with amino acids 1-446 of
SEQ ID NO: 7.
[0070] In other aspects of this embodiment, a BoNT/G light chain
comprises a polypeptide having, e.g., at most one, two, three,
four, five, six, seven, eight, nine, 10, 20, 30, 40, 50, 100, or
200 non-contiguous amino acid substitutions relative to amino acids
1-446 of SEQ ID NO: 7. In other aspects of this embodiment, a
BoNT/G light chain comprises a polypeptide having, e.g., at least
one, two, three, four, five, six, seven, eight, nine, 10, 20, 30,
40, 50, 100 or 200 non-contiguous amino acid substitutions relative
to amino acids 1-446 of SEQ ID NO: 7. In yet other aspects of this
embodiment, a BoNT/G light chain comprises a polypeptide having,
e.g., at most one, two, three, four, five, six, seven, eight, nine,
10, 20, 30, 40, 50, 100 or 200 non-contiguous amino acid deletions
relative to amino acids 1-446 of SEQ ID NO: 7. In other aspects of
this embodiment, a BoNT/G light chain comprises a polypeptide
having, e.g., at least one, two, three, four, five, six, seven,
eight, nine, 10, 20, 30, 40, 50, 100 or 200 non-contiguous amino
acid deletions relative to amino acids 1-446 of SEQ ID NO: 7. In
still other aspects of this embodiment, a BoNT/G light chain
comprises a polypeptide having, e.g., at most one, two, three,
four, five, six, seven, eight, nine, 10, 20, 30, 40, 50, 100 or 200
non-contiguous amino acid additions relative to amino acids 1-446
of SEQ ID NO: 7. In other aspects of this embodiment, a BoNT/G
light chain comprises a polypeptide having, e.g., at least one,
two, three, four, five, six, seven, eight, nine, 10, 20, 30, 40,
50, 100 or 200 non-contiguous amino acid additions relative to
amino acids 1-446 of SEQ ID NO: 7.
[0071] In other aspects of this embodiment, a BoNT/G light chain
comprises a polypeptide having, e.g., at most one, two, three,
four, five, six, seven, eight, nine, 10, 20, 30, 40, 50, 100 or 200
contiguous amino acid substitutions relative to amino acids 1-446
of SEQ ID NO: 7. In other aspects of this embodiment, a BoNT/G
light chain comprises a polypeptide having, e.g., at least one,
two, three, four, five, six, seven, eight, nine, 10, 20, 30, 40,
50, 100 or 200 contiguous amino acid substitutions relative to
amino acids 1-446 of SEQ ID NO: 7. In yet other aspects of this
embodiment, a BoNT/G light chain comprises a polypeptide having,
e.g., at most one, two, three, four, five, six, seven, eight, nine,
10, 20, 30, 40, 50, 100 or 200 contiguous amino acid deletions
relative to amino acids 1-446 of SEQ ID NO: 7. In other aspects of
this embodiment, a BoNT/G light chain comprises a polypeptide
having, e.g., at least one, two, three, four, five, six, seven,
eight, nine, 10, 20, 30, 40, 50, 100 or 200 contiguous amino acid
deletions relative to amino acids 1-446 of SEQ ID NO: 7. In still
other aspects of this embodiment, a BoNT/G light chain comprises a
polypeptide having, e.g., at most one, two, three, four, five, six,
seven, eight, nine, 10, 20, 30, 40, 50, 100 or 200 contiguous amino
acid additions relative to amino acids 1-446 of SEQ ID NO: 7. In
other aspects of this embodiment, a BoNT/G light chain comprises a
polypeptide having, e.g., at least one, two, three, four, five,
six, seven, eight, nine, 10, 20, 30, 40, 50, 100 or 200 contiguous
amino acid additions relative to amino acids 1-446 of SEQ ID NO:
7.
[0072] In another embodiment, a Clostridial toxin enzymatic domain
comprises a TeNT light chain. In an aspect of this embodiment, a
TeNT light chain comprises amino acids 1-457 of SEQ ID NO: 8. In
another aspect of this embodiment, a TeNT light chain comprises a
naturally occurring TeNT light chain variant, such as, e.g., a
light chain from a TeNT isoform or a light chain from a TeNT
subtype. In another aspect of this embodiment, a TeNT light chain
comprises amino acids 1-457 of a naturally occurring TeNT light
chain variant of SEQ ID NO: 8, such as, e.g., amino acids 1-457 of
a TeNT isoform of SEQ ID NO: 8 or amino acids 1-457 of a TeNT
subtype of SEQ ID NO: 8. In still another aspect of this
embodiment, a TeNT light chain comprises a non-naturally occurring
TeNT light chain variant, such as, e.g., a conservative TeNT light
chain variant, a non-conservative TeNT light chain variant, a TeNT
chimeric light chain, an active TeNT light chain fragment, or any
combination thereof. In still another aspect of this embodiment, a
TeNT light chain comprises amino acids 1-457 of a non-naturally
occurring TeNT light chain variant of SEQ ID NO: 8, such as, e.g.,
amino acids 1-457 of a conservative TeNT light chain variant of SEQ
ID NO: 8, amino acids 1-457 of a non-conservative TeNT light chain
variant of SEQ ID NO: 8, amino acids 1-457 of an active TeNT light
chain fragment of SEQ ID NO: 8, or any combination thereof.
[0073] In other aspects of this embodiment, a TeNT light chain
comprises a polypeptide having, e.g., at least 70% amino acid
identity with amino acids 1-457 of SEQ ID NO: 8, at least 75% amino
acid identity with amino acids 1-457 of SEQ ID NO: 8, at least 80%
amino acid identity with amino acids 1-457 of SEQ ID NO: 8, at
least 85% amino acid identity with amino acids 1-457 of SEQ ID NO:
8, at least 90% amino acid identity with amino acids 1-457 of SEQ
ID NO: 8 or at least 95% amino acid identity with amino acids 1-457
of SEQ ID NO: 8. In yet other aspects of this embodiment, a TeNT
light chain comprises a polypeptide having, e.g., at most 70% amino
acid identity with amino acids 1-457 of SEQ ID NO: 8, at most 75%
amino acid identity with amino acids 1-457 of SEQ ID NO: 8, at most
80% amino acid identity with amino acids 1-457 of SEQ ID NO: 8, at
most 85% amino acid identity with amino acids 1-457 of SEQ ID NO:
8, at most 90% amino acid identity with amino acids 1-457 of SEQ ID
NO: 8 or at most 95% amino acid identity with amino acids 1-457 of
SEQ ID NO: 8.
[0074] In other aspects of this embodiment, a TeNT light chain
comprises a polypeptide having, e.g., at most one, two, three,
four, five, six, seven, eight, nine, 10, 20, 30, 40, 50, 100, or
200 non-contiguous amino acid substitutions relative to amino acids
1-457 of SEQ ID NO: 8. In other aspects of this embodiment, a TeNT
light chain comprises a polypeptide having, e.g., at least one,
two, three, four, five, six, seven, eight, nine, 10, 20, 30, 40,
50, 100 or 200 non-contiguous amino acid substitutions relative to
amino acids 1-457 of SEQ ID NO: 8. In yet other aspects of this
embodiment, a TeNT light chain comprises a polypeptide having,
e.g., at most one, two, three, four, five, six, seven, eight, nine,
10, 20, 30, 40, 50, 100 or 200 non-contiguous amino acid deletions
relative to amino acids 1-457 of SEQ ID NO: 8. In other aspects of
this embodiment, a TeNT light chain comprises a polypeptide having,
e.g., at least one, two, three, four, five, six, seven, eight,
nine, 10, 20, 30, 40, 50, 100 or 200 non-contiguous amino acid
deletions relative to amino acids 1-457 of SEQ ID NO: 8. In still
other aspects of this embodiment, a TeNT light chain comprises a
polypeptide having, e.g., at most one, two, three, four, five, six,
seven, eight, nine, 10, 20, 30, 40, 50, 100 or 200 non-contiguous
amino acid additions relative to amino acids 1-457 of SEQ ID NO: 8.
In other aspects of this embodiment, a TeNT light chain comprises a
polypeptide having, e.g., at least one, two, three, four, five,
six, seven, eight, nine, 10, 20, 30, 40, 50, 100 or 200
non-contiguous amino acid additions relative to amino acids 1-457
of SEQ ID NO: 8.
[0075] In other aspects of this embodiment, a TeNT light chain
comprises a polypeptide having, e.g., at most one, two, three,
four, five, six, seven, eight, nine, 10, 20, 30, 40, 50, 100 or 200
contiguous amino acid substitutions relative to amino acids 1-457
of SEQ ID NO: 8. In other aspects of this embodiment, a TeNT light
chain comprises a polypeptide having, e.g., at least one, two,
three, four, five, six, seven, eight, nine, 10, 20, 30, 40, 50, 100
or 200 contiguous amino acid substitutions relative to amino acids
1-457 of SEQ ID NO: 8. In yet other aspects of this embodiment, a
TeNT light chain comprises a polypeptide having, e.g., at most one,
two, three, four, five, six, seven, eight, nine, 10, 20, 30, 40,
50, 100 or 200 contiguous amino acid deletions relative to amino
acids 1-457 of SEQ ID NO: 8. In other aspects of this embodiment, a
TeNT light chain comprises a polypeptide having, e.g., at least
one, two, three, four, five, six, seven, eight, nine, 10, 20, 30,
40, 50, 100 or 200 contiguous amino acid deletions relative to
amino acids 1-457 of SEQ ID NO: 8. In still other aspects of this
embodiment, a TeNT light chain comprises a polypeptide having,
e.g., at most one, two, three, four, five, six, seven, eight, nine,
10, 20, 30, 40, 50, 100 or 200 contiguous amino acid additions
relative to amino acids 1-457 of SEQ ID NO: 8. In other aspects of
this embodiment, a TeNT light chain comprises a polypeptide having,
e.g., at least one, two, three, four, five, six, seven, eight,
nine, 10, 20, 30, 40, 50, 100 or 200 contiguous amino acid
additions relative to amino acids 1-457 of SEQ ID NO: 8.
[0076] Aspects of the present invention provide, in part, a
Clostridial toxin translocation domain. As used herein, the term
"Clostridial toxin translocation domain" means any Clostridial
toxin polypeptide that can execute the translocation step of the
intoxication process that mediates Clostridial toxin light chain
translocation. Thus, a Clostridial toxin translocation domain
facilitates the movement of a Clostridial toxin light chain across
a membrane and encompasses the movement of a Clostridial toxin
light chain through the membrane an intracellular vesicle into the
cytoplasm of a cell. Non-limiting examples of a Clostridial toxin
translocation domain include, e.g., a Clostridial toxin H.sub.N
region such as, e.g., a BoNT/A H.sub.N region, a BoNT/B H.sub.N
region, a BoNT/C1 H.sub.N region, a BoNT/D H.sub.N region, a BoNT/E
H.sub.N region, a BoNT/F H.sub.N region, a BoNT/G H.sub.N region,
and a TeNT H.sub.N region.
[0077] A Clostridial toxin translocation domain includes, without
limitation, naturally occurring Clostridial toxin H.sub.N region
variants, such as, e.g., Clostridial toxin H.sub.N region isoforms
and Clostridial toxin H.sub.N region subtypes; non-naturally
occurring Clostridial toxin H.sub.N region variants, such as, e.g.,
conservative Clostridial toxin H.sub.N region variants,
non-conservative Clostridial toxin H.sub.N region variants,
Clostridial toxin H.sub.N region chimerics, active Clostridial
toxin H.sub.N region fragments thereof, or any combination
thereof.
[0078] As used herein, the term "Clostridial toxin H.sub.N region
variant," whether naturally-occurring or non-naturally-occurring,
means a Clostridial toxin H.sub.N region that has at least one
amino acid change from the corresponding region of the disclosed
reference sequences (see Table 1) and can be described in percent
identity to the corresponding region of that reference sequence.
Unless expressly indicated, all Clostridial toxin H.sub.N region
variants disclosed in the present specification are capable of
executing the translocation step of the intoxication process that
mediates Clostridial toxin light chain translocation. As
non-limiting examples, a BoNT/A H.sub.N region variant comprising
amino acids 449-873 of SEQ ID NO: 1 will have at least one amino
acid difference, such as, e.g., an amino acid substitution,
deletion or addition, as compared to the amino acid region 449-873
of SEQ ID NO: 1; a BoNT/B H.sub.N region variant comprising amino
acids 442-860 of SEQ ID NO: 2 will have at least one amino acid
difference, such as, e.g., an amino acid substitution, deletion or
addition, as compared to the amino acid region 442-860 of SEQ ID
NO: 2; a BoNT/C.sub.1H.sub.N region variant comprising amino acids
450-868 of SEQ ID NO: 3 will have at least one amino acid
difference, such as, e.g., an amino acid substitution, deletion or
addition, as compared to the amino acid region 450-868 of SEQ ID
NO: 3; a BoNT/D H.sub.N region variant comprising amino acids
446-864 of SEQ ID NO: 4 will have at least one amino acid
difference, such as, e.g., an amino acid substitution, deletion or
addition, as compared to the amino acid region 446-864 of SEQ ID
NO: 4; a BoNT/E H.sub.N region variant comprising amino acids
423-847 of SEQ ID NO: 5 will have at least one amino acid
difference, such as, e.g., an amino acid substitution, deletion or
addition, as compared to the amino acid region 423-847 of SEQ ID
NO: 5; a BoNT/F H.sub.N region variant comprising amino acids
440-866 of SEQ ID NO: 6 will have at least one amino acid
difference, such as, e.g., an amino acid substitution, deletion or
addition, as compared to the amino acid region 440-866 of SEQ ID
NO: 6; a BoNT/G H.sub.N region variant comprising amino acids
447-865 of SEQ ID NO: 7 will have at least one amino acid
difference, such as, e.g., an amino acid substitution, deletion or
addition, as compared to the amino acid region 447-865 of SEQ ID
NO: 7; and a TeNT H.sub.N region variant comprising amino acids
458-881 of SEQ ID NO: 8 will have at least one amino acid
difference, such as, e.g., an amino acid substitution, deletion or
addition, as compared to the amino acid region 458-881 of SEQ ID
NO: 8.
[0079] It is recognized by those of skill in the art that within
each serotype of Clostridial toxin there can be naturally occurring
Clostridial toxin H.sub.N region variants that differ somewhat in
their amino acid sequence, and also in the nucleic acids encoding
these proteins. For example, there are presently four BoNT/A
subtypes, BoNT/A1, BoNT/A2, BoNT/A3 and BoNT/A4, with specific
H.sub.N region subtypes showing approximately 87% amino acid
identity when compared to another BoNT/A H.sub.N region subtype. As
used herein, the term "naturally occurring Clostridial toxin
H.sub.N region variant" means any Clostridial toxin H.sub.N region
produced by a naturally-occurring process, including, without
limitation, Clostridial toxin H.sub.N region isoforms produced from
alternatively-spliced transcripts, Clostridial toxin H.sub.N region
isoforms produced by spontaneous mutation and Clostridial toxin
H.sub.N region subtypes. A naturally occurring Clostridial toxin
H.sub.N region variant can function in substantially the same
manner as the reference Clostridial toxin H.sub.N region on which
the naturally occurring Clostridial toxin H.sub.N region variant is
based, and can be substituted for the reference Clostridial toxin
H.sub.N region in any aspect of the present invention. A naturally
occurring Clostridial toxin H.sub.N region variant may substitute
one or more amino acids, two or more amino acids, three or more
amino acids, four or more amino acids, five or more amino acids,
ten or more amino acids, 20 or more amino acids, 30 or more amino
acids, 40 or more amino acids, 50 or more amino acids or 100 or
more amino acids from the reference Clostridial toxin H.sub.N
region on which the naturally occurring Clostridial toxin H.sub.N
region variant is based. A naturally occurring Clostridial toxin
H.sub.N region variant can also substitute at least 10 contiguous
amino acids, at least 15 contiguous amino acids, at least 20
contiguous amino acids, or at least 25 contiguous amino acids from
the reference Clostridial toxin H.sub.N region on which the
naturally occurring Clostridial toxin H.sub.N region variant is
based, that possess at least 50% amino acid identity, 65% amino
acid identity, 75% amino acid identity, 85% amino acid identity or
95% amino acid identity to the reference Clostridial toxin H.sub.N
region on which the naturally occurring Clostridial toxin H.sub.N
region variant is based.
[0080] A non-limiting examples of a naturally occurring Clostridial
toxin H.sub.N region variant is a Clostridial toxin H.sub.N region
isoform such as, e.g., a BoNT/A H.sub.N region isoform, a BoNT/B
H.sub.N region isoform, a BoNT/C1H.sub.N region isoform, a BoNT/D
H.sub.N region isoform, a BoNT/E H.sub.N region isoform, a BoNT/F
H.sub.N region isoform, a BoNT/G H.sub.N region isoform, and a TeNT
H.sub.N region isoform. A Clostridial toxin H.sub.N region isoform
can function in substantially the same manner as the reference
Clostridial toxin H.sub.N region on which the Clostridial toxin
H.sub.N region isoform is based, and can be substituted for the
reference Clostridial toxin H.sub.N region in any aspect of the
present invention.
[0081] Another non-limiting examples of a naturally occurring
Clostridial toxin H.sub.N region variant is a Clostridial toxin
H.sub.N region subtype such as, e.g., a H.sub.N region from subtype
BoNT/A1, BoNT/A2, BoNT/A3 and BoNT/A4; a H.sub.N region from
subtype BoNT/B1, BoNT/B2, BoNT/B bivalent and BoNT/B
nonproteolytic; a H.sub.N region from subtype BoNT/C1-1 and
BoNT/C1-2; a H.sub.N region from subtype BoNT/E1, BoNT/E2 and
BoNT/E3; and a H.sub.N region from subtype BoNT/F1, BoNT/F2,
BoNT/F3 and BoNT/F4. A Clostridial toxin H.sub.N region subtype can
function in substantially the same manner as the reference
Clostridial toxin H.sub.N region on which the Clostridial toxin
H.sub.N region subtype is based, and can be substituted for the
reference Clostridial toxin H.sub.N region in any aspect of the
present invention.
[0082] As used herein, the term "non-naturally occurring
Clostridial toxin H.sub.N region variant" means any Clostridial
toxin H.sub.N region produced with the aid of human manipulation,
including, without limitation, Clostridial toxin H.sub.N regions
produced by genetic engineering using random mutagenesis or
rational design and Clostridial toxin H.sub.N regions produced by
chemical synthesis. Non-limiting examples of non-naturally
occurring Clostridial toxin H.sub.N region variants include, e.g.,
conservative Clostridial toxin H.sub.N region variants,
non-conservative Clostridial toxin H.sub.N region variants,
Clostridial toxin H.sub.N region chimeric variants and active
Clostridial toxin H.sub.N region fragments.
[0083] As used herein, the term "conservative Clostridial toxin
H.sub.N region variant" means a Clostridial toxin H.sub.N region
that has at least one amino acid substituted by another amino acid
or an amino acid analog that has at least one property similar to
that of the original amino acid from the reference Clostridial
toxin H.sub.N region sequence (Table 1). Examples of properties
include, without limitation, similar size, topography, charge,
hydrophobicity, hydrophilicity, lipophilicity, covalent-bonding
capacity, hydrogen-bonding capacity, a physicochemical property, of
the like, or any combination thereof. A conservative Clostridial
toxin H.sub.N region variant can function in substantially the same
manner as the reference Clostridial toxin H.sub.N region on which
the conservative Clostridial toxin H.sub.N region variant is based,
and can be substituted for the reference Clostridial toxin H.sub.N
region in any aspect of the present invention. A conservative
Clostridial toxin H.sub.N region variant may substitute one or more
amino acids, two or more amino acids, three or more amino acids,
four or more amino acids, five or more amino acids, ten or more
amino acids, 20 or more amino acids, 30 or more amino acids, 40 or
more amino acids, 50 or more amino acids, 100 or more amino acids
or 200 or more amino acids from the reference Clostridial toxin
H.sub.N region on which the conservative Clostridial toxin H.sub.N
region variant is based. A conservative Clostridial toxin H.sub.N
region variant can also substitute at least 10 contiguous amino
acids, at least 15 contiguous amino acids, at least 20 contiguous
amino acids, or at least 25 contiguous amino acids from the
reference Clostridial toxin H.sub.N region on which the
conservative Clostridial toxin H.sub.N region variant is based,
that possess at least 50% amino acid identity, 65% amino acid
identity, 75% amino acid identity, 85% amino acid identity or 95%
amino acid identity to the reference Clostridial toxin H.sub.N
region on which the conservative Clostridial toxin H.sub.N region
variant is based. Non-limiting examples of a conservative
Clostridial toxin H.sub.N region variant include, e.g.,
conservative BoNT/A H.sub.N region variants, conservative BoNT/B
H.sub.N region variants, conservative BoNT/C1 H.sub.N region
variants, conservative BoNT/D H.sub.N region variants, conservative
BoNT/E H.sub.N region variants, conservative BoNT/F H.sub.N region
variants, conservative BoNT/G H.sub.N region variants, and
conservative TeNT H.sub.N region variants.
[0084] As used herein, the term "non-conservative Clostridial toxin
H.sub.N region variant" means a Clostridial toxin H.sub.N region in
which 1) at least one amino acid is deleted from the reference
Clostridial toxin H.sub.N region on which the non-conservative
Clostridial toxin H.sub.N region variant is based; 2) at least one
amino acid added to the reference Clostridial toxin H.sub.N region
on which the non-conservative Clostridial toxin H.sub.N region is
based; or 3) at least one amino acid is substituted by another
amino acid or an amino acid analog that does not share any property
similar to that of the original amino acid from the reference
Clostridial toxin H.sub.N region sequence (Table 1). A
non-conservative Clostridial toxin H.sub.N region variant can
function in substantially the same manner as the reference
Clostridial toxin H.sub.N region on which the non-conservative
Clostridial toxin H.sub.N region variant is based, and can be
substituted for the reference Clostridial toxin H.sub.N region in
any aspect of the present invention. A non-conservative Clostridial
toxin H.sub.N region variant can delete one or more amino acids,
two or more amino acids, three or more amino acids, four or more
amino acids, five or more amino acids, and ten or more amino acids
from the reference Clostridial toxin H.sub.N region on which the
non-conservative Clostridial toxin H.sub.N region variant is based.
A non-conservative Clostridial toxin H.sub.N region variant can add
one or more amino acids, two or more amino acids, three or more
amino acids, four or more amino acids, five or more amino acids,
and ten or more amino acids to the reference Clostridial toxin
H.sub.N region on which the non-conservative Clostridial toxin
H.sub.N region variant is based. A non-conservative Clostridial
toxin H.sub.N region variant may substitute one or more amino
acids, two or more amino acids, three or more amino acids, four or
more amino acids, five or more amino acids, ten or more amino
acids, 20 or more amino acids, 30 or more amino acids, 40 or more
amino acids, 50 or more amino acids, 100 or more amino acids or 200
or more amino acids from the reference Clostridial toxin H.sub.N
region on which the non-conservative Clostridial toxin H.sub.N
region variant is based. A non-conservative Clostridial toxin
H.sub.N region variant can also substitute at least 10 contiguous
amino acids, at least 15 contiguous amino acids, at least 20
contiguous amino acids, or at least 25 contiguous amino acids from
the reference Clostridial toxin H.sub.N region on which the
non-conservative Clostridial toxin H.sub.N region variant is based,
that possess at least 50% amino acid identity, 65% amino acid
identity, 75% amino acid identity, 85% amino acid identity or 95%
amino acid identity to the reference Clostridial toxin H.sub.N
region on which the non-conservative Clostridial toxin H.sub.N
region variant is based. Non-limiting examples of a
non-conservative Clostridial toxin H.sub.N region variant include,
e.g., non-conservative BoNT/A H.sub.N region variants,
non-conservative BoNT/B H.sub.N region variants, non-conservative
BoNT/C1 H.sub.N region variants, non-conservative BoNT/D H.sub.N
region variants, non-conservative BoNT/E H.sub.N region variants,
non-conservative BoNT/F H.sub.N region variants, non-conservative
BoNT/G H.sub.N region variants, and non-conservative TeNT H.sub.N
region variants.
[0085] As used herein, the term "Clostridial toxin H.sub.N region
chimeric" means a polypeptide comprising at least a portion of a
Clostridial toxin H.sub.N region and at least a portion of at least
one other polypeptide to form a toxin H.sub.N region with at least
one property different from the reference Clostridial toxin H.sub.N
regions of Table 1, with the proviso that this Clostridial toxin
H.sub.N region chimeric is still capable of facilitating the
release of the LC from intracellular vesicles into the cytoplasm of
the target cell and thus participate in executing the overall
cellular mechanism whereby a Clostridial toxin proteolytically
cleaves a substrate.
[0086] As used herein, the term "active Clostridial toxin H.sub.N
region fragment" means any of a variety of Clostridial toxin
fragments comprising the H.sub.N region can be useful in aspects of
the present invention with the proviso that these active fragments
can facilitate the release of the LC from intracellular vesicles
into the cytoplasm of the target cell and thus participate in
executing the overall cellular mechanism whereby a Clostridial
toxin proteolytically cleaves a substrate. The H.sub.N regions from
the heavy chains of Clostridial toxins are approximately 410-430
amino acids in length and comprise a translocation domain (Table
1). Research has shown that the entire length of a H.sub.N region
from a Clostridial toxin heavy chain is not necessary for the
translocating activity of the translocation domain. Thus, aspects
of this embodiment can include Clostridial toxin H.sub.N regions
comprising a translocation domain having a length of, e.g., at
least 350 amino acids, at least 375 amino acids, at least 400 amino
acids and at least 425 amino acids. Other aspects of this
embodiment can include Clostridial toxin H.sub.N regions comprising
translocation domain having a length of, e.g., at most 350 amino
acids, at most 375 amino acids, at most 400 amino acids and at most
425 amino acids.
[0087] Any of a variety of sequence alignment methods can be used
to determine percent identity of naturally-occurring Clostridial
toxin H.sub.N region variants and non-naturally-occurring
Clostridial toxin H.sub.N region variants, including, without
limitation, global methods, local methods and hybrid methods, such
as, e.g., segment approach methods. Protocols to determine percent
identity are routine procedures within the scope of one skilled in
the art and from the teaching herein.
[0088] Thus, in an embodiment, a modified Clostridial toxin
disclosed in the present specification comprises a Clostridial
toxin translocation domain. In an aspect of this embodiment, a
Clostridial toxin translocation domain comprises a naturally
occurring Clostridial toxin H.sub.N region variant, such as, e.g.,
a Clostridial toxin H.sub.N region isoform or a Clostridial toxin
H.sub.N region subtype. In another aspect of this embodiment, a
Clostridial toxin translocation domain comprises a non-naturally
occurring Clostridial toxin H.sub.N region variant, such as, e.g.,
a conservative Clostridial toxin H.sub.N region variant, a
non-conservative Clostridial toxin H.sub.N region variant, a
Clostridial toxin chimeric H.sub.N region, an active Clostridial
toxin H.sub.N region fragment, or any combination thereof.
[0089] In another embodiment, a Clostridial toxin translocation
domain comprises a BoNT/A H.sub.N region. In an aspect of this
embodiment, a BoNT/A H.sub.N region comprises amino acids 449-873
of SEQ ID NO: 1. In another aspect of this embodiment, a BoNT/A
H.sub.N region comprises a naturally occurring BoNT/A H.sub.N
region variant, such as, e.g., a H.sub.N region from a BoNT/A
isoform or a H.sub.N region from a BoNT/A subtype. In another
aspect of this embodiment, a BoNT/A H.sub.N region comprises amino
acids 449-873 of a naturally occurring BoNT/A H.sub.N region
variant of SEQ ID NO: 1, such as, e.g., amino acids 449-873 of a
BoNT/A isoform of SEQ ID NO: 1 or amino acids 449-873 of a BoNT/A
subtype of SEQ ID NO: 1. In still another aspect of this
embodiment, a BoNT/A H.sub.N region comprises a non-naturally
occurring BoNT/A H.sub.N region variant, such as, e.g., a
conservative BoNT/A H.sub.N region variant, a non-conservative
BoNT/A H.sub.N region variant, a BoNT/A chimeric H.sub.N region, an
active BoNT/A H.sub.N region fragment, or any combination thereof.
In still another aspect of this embodiment, a BoNT/A H.sub.N region
comprises amino acids 449-873 of a non-naturally occurring BoNT/A
H.sub.N region variant of SEQ ID NO: 1, such as, e.g., amino acids
449-873 of a conservative BoNT/A H.sub.N region variant of SEQ ID
NO: 1, amino acids 449-873 of a non-conservative BoNT/A H.sub.N
region variant of SEQ ID NO: 1, amino acids 449-873 of an active
BoNT/A H.sub.N region fragment of SEQ ID NO: 1, or any combination
thereof.
[0090] In other aspects of this embodiment, a BoNT/A H.sub.N region
comprises a polypeptide having, e.g., at least 70% amino acid
identity with amino acids 449-873 of SEQ ID NO: 1, at least 75%
amino acid identity with amino acids 449-873 of SEQ ID NO: 1, at
least 80% amino acid identity with amino acids 449-873 of SEQ ID
NO: 1, at least 85% amino acid identity with amino acids 449-873 of
SEQ ID NO: 1, at least 90% amino acid identity with amino acids
449-873 of SEQ ID NO: 1 or at least 95% amino acid identity with
amino acids 449-873 of SEQ ID NO: 1. In yet other aspects of this
embodiment, a BoNT/A H.sub.N region comprises a polypeptide having,
e.g., at most 70% amino acid identity with amino acids 449-873 of
SEQ ID NO: 1, at most 75% amino acid identity with amino acids
449-873 of SEQ ID NO: 1, at most 80% amino acid identity with amino
acids 449-873 of SEQ ID NO: 1, at most 85% amino acid identity with
amino acids 449-873 of SEQ ID NO: 1, at most 90% amino acid
identity with amino acids 449-873 of SEQ ID NO: 1 or at most 95%
amino acid identity with amino acids 449-873 of SEQ ID NO: 1.
[0091] In other aspects of this embodiment, a BoNT/A H.sub.N region
comprises a polypeptide having, e.g., at most one, two, three,
four, five, six, seven, eight, nine, 10, 20, 30, 40, 50, 100, or
200 non-contiguous amino acid substitutions relative to amino acids
449-873 of SEQ ID NO: 1. In other aspects of this embodiment, a
BoNT/A H.sub.N region comprises a polypeptide having, e.g., at
least one, two, three, four, five, six, seven, eight, nine, 10, 20,
30, 40, 50, 100 or 200 non-contiguous amino acid substitutions
relative to amino acids 449-873 of SEQ ID NO: 1. In yet other
aspects of this embodiment, a BoNT/A H.sub.N region comprises a
polypeptide having, e.g., at most one, two, three, four, five, six,
seven, eight, nine, 10, 20, 30, 40, 50, 100 or 200 non-contiguous
amino acid deletions relative to amino acids 449-873 of SEQ ID NO:
1. In other aspects of this embodiment, a BoNT/A H.sub.N region
comprises a polypeptide having, e.g., at least one, two, three,
four, five, six, seven, eight, nine, 10, 20, 30, 40, 50, 100 or 200
non-contiguous amino acid deletions relative to amino acids 449-873
of SEQ ID NO: 1. In still other aspects of this embodiment, a
BoNT/A H.sub.N region comprises a polypeptide having, e.g., at most
one, two, three, four, five, six, seven, eight, nine, 10, 20, 30,
40, 50, 100 or 200 non-contiguous amino acid additions relative to
amino acids 449-873 of SEQ ID NO: 1. In other aspects of this
embodiment, a BoNT/A H.sub.N region comprises a polypeptide having,
e.g., at least one, two, three, four, five, six, seven, eight,
nine, 10, 20, 30, 40, 50, 100 or 200 non-contiguous amino acid
additions relative to amino acids 449-873 of SEQ ID NO: 1.
[0092] In other aspects of this embodiment, a BoNT/A H.sub.N region
comprises a polypeptide having, e.g., at most one, two, three,
four, five, six, seven, eight, nine, 10, 20, 30, 40, 50, 100 or 200
contiguous amino acid substitutions relative to amino acids 449-873
of SEQ ID NO: 1. In other aspects of this embodiment, a BoNT/A
H.sub.N region comprises a polypeptide having, e.g., at least one,
two, three, four, five, six, seven, eight, nine, 10, 20, 30, 40,
50, 100 or 200 contiguous amino acid substitutions relative to
amino acids 449-873 of SEQ ID NO: 1. In yet other aspects of this
embodiment, a BoNT/A H.sub.N region comprises a polypeptide having,
e.g., at most one, two, three, four, five, six, seven, eight, nine,
10, 20, 30, 40, 50, 100 or 200 contiguous amino acid deletions
relative to amino acids 449-873 of SEQ ID NO: 1. In other aspects
of this embodiment, a BoNT/A H.sub.N region comprises a polypeptide
having, e.g., at least one, two, three, four, five, six, seven,
eight, nine, 10, 20, 30, 40, 50, 100 or 200 contiguous amino acid
deletions relative to amino acids 449-873 of SEQ ID NO: 1. In still
other aspects of this embodiment, a BoNT/A H.sub.N region comprises
a polypeptide having, e.g., at most one, two, three, four, five,
six, seven, eight, nine, 10, 20, 30, 40, 50, 100 or 200 contiguous
amino acid additions relative to amino acids 449-873 of SEQ ID NO:
1. In other aspects of this embodiment, a BoNT/A H.sub.N region
comprises a polypeptide having, e.g., at least one, two, three,
four, five, six, seven, eight, nine, 10, 20, 30, 40, 50, 100 or 200
contiguous amino acid additions relative to amino acids 449-873 of
SEQ ID NO: 1.
[0093] In another embodiment, a Clostridial toxin translocation
domain comprises a BoNT/B H.sub.N region. In an aspect of this
embodiment, a BoNT/B H.sub.N region comprises amino acids 442-860
of SEQ ID NO: 2. In another aspect of this embodiment, a BoNT/B
H.sub.N region comprises a naturally occurring BoNT/B H.sub.N
region variant, such as, e.g., a H.sub.N region from a BoNT/B
isoform or a H.sub.N region from a BoNT/B subtype. In another
aspect of this embodiment, a BoNT/B H.sub.N region comprises amino
acids 442-860 of a naturally occurring BoNT/B H.sub.N region
variant of SEQ ID NO: 2, such as, e.g., amino acids 442-860 of a
BoNT/B isoform of SEQ ID NO: 2 or amino acids 442-860 of a BoNT/B
subtype of SEQ ID NO: 2. In still another aspect of this
embodiment, a BoNT/B H.sub.N region comprises a non-naturally
occurring BoNT/B H.sub.N region variant, such as, e.g., a
conservative BoNT/B H.sub.N region variant, a non-conservative
BoNT/B H.sub.N region variant, a BoNT/B chimeric H.sub.N region, an
active BoNT/B H.sub.N region fragment, or any combination thereof.
In still another aspect of this embodiment, a BoNT/B H.sub.N region
comprises amino acids 442-860 of a non-naturally occurring BoNT/B
H.sub.N region variant of SEQ ID NO: 2, such as, e.g., amino acids
442-860 of a conservative BoNT/B H.sub.N region variant of SEQ ID
NO: 2, amino acids 442-860 of a non-conservative BoNT/B H.sub.N
region variant of SEQ ID NO: 2, amino acids 442-860 of an active
BoNT/B H.sub.N region fragment of SEQ ID NO: 2, or any combination
thereof.
[0094] In other aspects of this embodiment, a BoNT/B H.sub.N region
comprises a polypeptide having, e.g., at least 70% amino acid
identity with amino acids 442-860 of SEQ ID NO: 2, at least 75%
amino acid identity with amino acids 442-860 of SEQ ID NO: 2, at
least 80% amino acid identity with amino acids 442-860 of SEQ ID
NO: 2, at least 85% amino acid identity with amino acids 442-860 of
SEQ ID NO: 2, at least 90% amino acid identity with amino acids
442-860 of SEQ ID NO: 2 or at least 95% amino acid identity with
amino acids 442-860 of SEQ ID NO: 2. In yet other aspects of this
embodiment, a BoNT/B H.sub.N region comprises a polypeptide having,
e.g., at most 70% amino acid identity with amino acids 442-860 of
SEQ ID NO: 2, at most 75% amino acid identity with amino acids
442-860 of SEQ ID NO: 2, at most 80% amino acid identity with amino
acids 442-860 of SEQ ID NO: 2, at most 85% amino acid identity with
amino acids 442-860 of SEQ ID NO: 2, at most 90% amino acid
identity with amino acids 442-860 of SEQ ID NO: 2 or at most 95%
amino acid identity with amino acids 442-860 of SEQ ID NO: 2.
[0095] In other aspects of this embodiment, a BoNT/B H.sub.N region
comprises a polypeptide having, e.g., at most one, two, three,
four, five, six, seven, eight, nine, 10, 20, 30, 40, 50, 100, or
200 non-contiguous amino acid substitutions relative to amino acids
442-860 of SEQ ID NO: 2. In other aspects of this embodiment, a
BoNT/B H.sub.N region comprises a polypeptide having, e.g., at
least one, two, three, four, five, six, seven, eight, nine, 10, 20,
30, 40, 50, 100 or 200 non-contiguous amino acid substitutions
relative to amino acids 442-860 of SEQ ID NO: 2. In yet other
aspects of this embodiment, a BoNT/B H.sub.N region comprises a
polypeptide having, e.g., at most one, two, three, four, five, six,
seven, eight, nine, 10, 20, 30, 40, 50, 100 or 200 non-contiguous
amino acid deletions relative to amino acids 442-860 of SEQ ID NO:
2. In other aspects of this embodiment, a BoNT/B H.sub.N region
comprises a polypeptide having, e.g., at least one, two, three,
four, five, six, seven, eight, nine, 10, 20, 30, 40, 50, 100 or 200
non-contiguous amino acid deletions relative to amino acids 442-860
of SEQ ID NO: 2. In still other aspects of this embodiment, a
BoNT/B H.sub.N region comprises a polypeptide having, e.g., at most
one, two, three, four, five, six, seven, eight, nine, 10, 20, 30,
40, 50, 100 or 200 non-contiguous amino acid additions relative to
amino acids 442-860 of SEQ ID NO: 2. In other aspects of this
embodiment, a BoNT/B H.sub.N region comprises a polypeptide having,
e.g., at least one, two, three, four, five, six, seven, eight,
nine, 10, 20, 30, 40, 50, 100 or 200 non-contiguous amino acid
additions relative to amino acids 442-860 of SEQ ID NO: 2.
[0096] In other aspects of this embodiment, a BoNT/B H.sub.N region
comprises a polypeptide having, e.g., at most one, two, three,
four, five, six, seven, eight, nine, 10, 20, 30, 40, 50, 100 or 200
contiguous amino acid substitutions relative to amino acids 442-860
of SEQ ID NO: 2. In other aspects of this embodiment, a BoNT/B
H.sub.N region comprises a polypeptide having, e.g., at least one,
two, three, four, five, six, seven, eight, nine, 10, 20, 30, 40,
50, 100 or 200 contiguous amino acid substitutions relative to
amino acids 442-860 of SEQ ID NO: 2. In yet other aspects of this
embodiment, a BoNT/B H.sub.N region comprises a polypeptide having,
e.g., at most one, two, three, four, five, six, seven, eight, nine,
10, 20, 30, 40, 50, 100 or 200 contiguous amino acid deletions
relative to amino acids 442-860 of SEQ ID NO: 2. In other aspects
of this embodiment, a BoNT/B H.sub.N region comprises a polypeptide
having, e.g., at least one, two, three, four, five, six, seven,
eight, nine, 10, 20, 30, 40, 50, 100 or 200 contiguous amino acid
deletions relative to amino acids 442-860 of SEQ ID NO: 2. In still
other aspects of this embodiment, a BoNT/B H.sub.N region comprises
a polypeptide having, e.g., at most one, two, three, four, five,
six, seven, eight, nine, 10, 20, 30, 40, 50, 100 or 200 contiguous
amino acid additions relative to amino acids 442-860 of SEQ ID NO:
2. In other aspects of this embodiment, a BoNT/B H.sub.N region
comprises a polypeptide having, e.g., at least one, two, three,
four, five, six, seven, eight, nine, 10, 20, 30, 40, 50, 100 or 200
contiguous amino acid additions relative to amino acids 442-860 of
SEQ ID NO: 2.
[0097] In another embodiment, a Clostridial toxin translocation
domain comprises a BoNT/C1 H.sub.N region. In an aspect of this
embodiment, a BoNT/C1 H.sub.N region comprises amino acids 450-868
of SEQ ID NO: 3. In another aspect of this embodiment, a BoNT/C1
H.sub.N region comprises a naturally occurring BoNT/C1 H.sub.N
region variant, such as, e.g., a H.sub.N region from a BoNT/C1
isoform or a H.sub.N region from a BoNT/C1 subtype. In another
aspect of this embodiment, a BoNT/C1 H.sub.N region comprises amino
acids 450-868 of a naturally occurring BoNT/C1 H.sub.N region
variant of SEQ ID NO: 3, such as, e.g., amino acids 450-868 of a
BoNT/C1 isoform of SEQ ID NO: 3 or amino acids 450-868 of a BoNT/C1
subtype of SEQ ID NO: 3. In still another aspect of this
embodiment, a BoNT/C1 H.sub.N region comprises a non-naturally
occurring BoNT/C1 H.sub.N region variant, such as, e.g., a
conservative BoNT/C1 H.sub.N region variant, a non-conservative
BoNT/C1 H.sub.N region variant, a BoNT/C1 chimeric H.sub.N region,
an active BoNT/C1 H.sub.N region fragment, or any combination
thereof. In still another aspect of this embodiment, a BoNT/C1
H.sub.N region comprises amino acids 450-868 of a non-naturally
occurring BoNT/C1 H.sub.N region variant of SEQ ID NO: 3, such as,
e.g., amino acids 450-868 of a conservative BoNT/C1 H.sub.N region
variant of SEQ ID NO: 3, amino acids 450-868 of a non-conservative
BoNT/C1 H.sub.N region variant of SEQ ID NO: 3, amino acids 450-868
of an active BoNT/C1 H.sub.N region fragment of SEQ ID NO: 3, or
any combination thereof.
[0098] In other aspects of this embodiment, a BoNT/C1 H.sub.N
region comprises a polypeptide having, e.g., at least 70% amino
acid identity with amino acids 450-868 of SEQ ID NO: 3, at least
75% amino acid identity with amino acids 450-868 of SEQ ID NO: 3,
at least 80% amino acid identity with amino acids 450-868 of SEQ ID
NO: 3, at least 85% amino acid identity with amino acids 450-868 of
SEQ ID NO: 3, at least 90% amino acid identity with amino acids
450-868 of SEQ ID NO: 3 or at least 95% amino acid identity with
amino acids 450-868 of SEQ ID NO: 3. In yet other aspects of this
embodiment, a BoNT/C1H.sub.N region comprises a polypeptide having,
e.g., at most 70% amino acid identity with amino acids 450-868 of
SEQ ID NO: 3, at most 75% amino acid identity with amino acids
450-868 of SEQ ID NO: 3, at most 80% amino acid identity with amino
acids 450-868 of SEQ ID NO: 3, at most 85% amino acid identity with
amino acids 450-868 of SEQ ID NO: 3, at most 90% amino acid
identity with amino acids 450-868 of SEQ ID NO: 3 or at most 95%
amino acid identity with amino acids 450-868 of SEQ ID NO: 3.
[0099] In other aspects of this embodiment, a BoNT/C1 H.sub.N
region comprises a polypeptide having, e.g., at most one, two,
three, four, five, six, seven, eight, nine, 10, 20, 30, 40, 50,
100, or 200 non-contiguous amino acid substitutions relative to
amino acids 450-868 of SEQ ID NO: 3. In other aspects of this
embodiment, a BoNT/C1 H.sub.N region comprises a polypeptide
having, e.g., at least one, two, three, four, five, six, seven,
eight, nine, 10, 20, 30, 40, 50, 100 or 200 non-contiguous amino
acid substitutions relative to amino acids 450-868 of SEQ ID NO: 3.
In yet other aspects of this embodiment, a BoNT/C1H.sub.N region
comprises a polypeptide having, e.g., at most one, two, three,
four, five, six, seven, eight, nine, 10, 20, 30, 40, 50, 100 or 200
non-contiguous amino acid deletions relative to amino acids 450-868
of SEQ ID NO: 3. In other aspects of this embodiment, a BoNT/C1
H.sub.N region comprises a polypeptide having, e.g., at least one,
two, three, four, five, six, seven, eight, nine, 10, 20, 30, 40,
50, 100 or 200 non-contiguous amino acid deletions relative to
amino acids 450-868 of SEQ ID NO: 3. In still other aspects of this
embodiment, a BoNT/C1 H.sub.N region comprises a polypeptide
having, e.g., at most one, two, three, four, five, six, seven,
eight, nine, 10, 20, 30, 40, 50, 100 or 200 non-contiguous amino
acid additions relative to amino acids 450-868 of SEQ ID NO: 3. In
other aspects of this embodiment, a BoNT/C1 H.sub.N region
comprises a polypeptide having, e.g., at least one, two, three,
four, five, six, seven, eight, nine, 10, 20, 30, 40, 50, 100 or 200
non-contiguous amino acid additions relative to amino acids 450-868
of SEQ ID NO: 3.
[0100] In other aspects of this embodiment, a BoNT/C1 H.sub.N
region comprises a polypeptide having, e.g., at most one, two,
three, four, five, six, seven, eight, nine, 10, 20, 30, 40, 50, 100
or 200 contiguous amino acid substitutions relative to amino acids
450-868 of SEQ ID NO: 3. In other aspects of this embodiment, a
BoNT/C1 H.sub.N region comprises a polypeptide having, e.g., at
least one, two, three, four, five, six, seven, eight, nine, 10, 20,
30, 40, 50, 100 or 200 contiguous amino acid substitutions relative
to amino acids 450-868 of SEQ ID NO: 3. In yet other aspects of
this embodiment, a BoNT/C1 H.sub.N region comprises a polypeptide
having, e.g., at most one, two, three, four, five, six, seven,
eight, nine, 10, 20, 30, 40, 50, 100 or 200 contiguous amino acid
deletions relative to amino acids 450-868 of SEQ ID NO: 3. In other
aspects of this embodiment, a BoNT/C1 H.sub.N region comprises a
polypeptide having, e.g., at least one, two, three, four, five,
six, seven, eight, nine, 10, 20, 30, 40, 50, 100 or 200 contiguous
amino acid deletions relative to amino acids 450-868 of SEQ ID NO:
3. In still other aspects of this embodiment, a BoNT/C1 H.sub.N
region comprises a polypeptide having, e.g., at most one, two,
three, four, five, six, seven, eight, nine, 10, 20, 30, 40, 50, 100
or 200 contiguous amino acid additions relative to amino acids
450-868 of SEQ ID NO: 3. In other aspects of this embodiment, a
BoNT/C1 H.sub.N region comprises a polypeptide having, e.g., at
least one, two, three, four, five, six, seven, eight, nine, 10, 20,
30, 40, 50, 100 or 200 contiguous amino acid additions relative to
amino acids 450-868 of SEQ ID NO: 3.
[0101] In another embodiment, a Clostridial toxin translocation
domain comprises a BoNT/D H.sub.N region. In an aspect of this
embodiment, a BoNT/D H.sub.N region comprises amino acids 446-864
of SEQ ID NO: 4. In another aspect of this embodiment, a BoNT/D
H.sub.N region comprises a naturally occurring BoNT/D H.sub.N
region variant, such as, e.g., a H.sub.N region from a BoNT/D
isoform or a H.sub.N region from a BoNT/D subtype. In another
aspect of this embodiment, a BoNT/D H.sub.N region comprises amino
acids 446-864 of a naturally occurring BoNT/D H.sub.N region
variant of SEQ ID NO: 4, such as, e.g., amino acids 446-864 of a
BoNT/D isoform of SEQ ID NO: 4 or amino acids 446-864 of a BoNT/D
subtype of SEQ ID NO: 4. In still another aspect of this
embodiment, a BoNT/D H.sub.N region comprises a non-naturally
occurring BoNT/D H.sub.N region variant, such as, e.g., a
conservative BoNT/D H.sub.N region variant, a non-conservative
BoNT/D H.sub.N region variant, a BoNT/D chimeric H.sub.N region, an
active BoNT/D H.sub.N region fragment, or any combination thereof.
In still another aspect of this embodiment, a BoNT/D H.sub.N region
comprises amino acids 446-864 of a non-naturally occurring BoNT/D
H.sub.N region variant of SEQ ID NO: 4, such as, e.g., amino acids
446-864 of a conservative BoNT/D H.sub.N region variant of SEQ ID
NO: 4, amino acids 446-864 of a non-conservative BoNT/D H.sub.N
region variant of SEQ ID NO: 4, amino acids 446-864 of an active
BoNT/D H.sub.N region fragment of SEQ ID NO: 4, or any combination
thereof.
[0102] In other aspects of this embodiment, a BoNT/D H.sub.N region
comprises a polypeptide having, e.g., at least 70% amino acid
identity with amino acids 446-864 of SEQ ID NO: 4, at least 75%
amino acid identity with amino acids 446-864 of SEQ ID NO: 4, at
least 80% amino acid identity with amino acids 446-864 of SEQ ID
NO: 4, at least 85% amino acid identity with amino acids 446-864 of
SEQ ID NO: 4, at least 90% amino acid identity with amino acids
446-864 of SEQ ID NO: 4 or at least 95% amino acid identity with
amino acids 446-864 of SEQ ID NO: 4. In yet other aspects of this
embodiment, a BoNT/D H.sub.N region comprises a polypeptide having,
e.g., at most 70% amino acid identity with amino acids 446-864 of
SEQ ID NO: 4, at most 75% amino acid identity with amino acids
446-864 of SEQ ID NO: 4, at most 80% amino acid identity with amino
acids 446-864 of SEQ ID NO: 4, at most 85% amino acid identity with
amino acids 446-864 of SEQ ID NO: 4, at most 90% amino acid
identity with amino acids 446-864 of SEQ ID NO: 4 or at most 95%
amino acid identity with amino acids 446-864 of SEQ ID NO: 4.
[0103] In other aspects of this embodiment, a BoNT/D H.sub.N region
comprises a polypeptide having, e.g., at most one, two, three,
four, five, six, seven, eight, nine, 10, 20, 30, 40, 50, 100, or
200 non-contiguous amino acid substitutions relative to amino acids
446-864 of SEQ ID NO: 4. In other aspects of this embodiment, a
BoNT/D H.sub.N region comprises a polypeptide having, e.g., at
least one, two, three, four, five, six, seven, eight, nine, 10, 20,
30, 40, 50, 100 or 200 non-contiguous amino acid substitutions
relative to amino acids 446-864 of SEQ ID NO: 4. In yet other
aspects of this embodiment, a BoNT/D H.sub.N region comprises a
polypeptide having, e.g., at most one, two, three, four, five, six,
seven, eight, nine, 10, 20, 30, 40, 50, 100 or 200 non-contiguous
amino acid deletions relative to amino acids 446-864 of SEQ ID NO:
4. In other aspects of this embodiment, a BoNT/D H.sub.N region
comprises a polypeptide having, e.g., at least one, two, three,
four, five, six, seven, eight, nine, 10, 20, 30, 40, 50, 100 or 200
non-contiguous amino acid deletions relative to amino acids 446-864
of SEQ ID NO: 4. In still other aspects of this embodiment, a
BoNT/D H.sub.N region comprises a polypeptide having, e.g., at most
one, two, three, four, five, six, seven, eight, nine, 10, 20, 30,
40, 50, 100 or 200 non-contiguous amino acid additions relative to
amino acids 446-864 of SEQ ID NO: 4. In other aspects of this
embodiment, a BoNT/D H.sub.N region comprises a polypeptide having,
e.g., at least one, two, three, four, five, six, seven, eight,
nine, 10, 20, 30, 40, 50, 100 or 200 non-contiguous amino acid
additions relative to amino acids 446-864 of SEQ ID NO: 4.
[0104] In other aspects of this embodiment, a BoNT/D H.sub.N region
comprises a polypeptide having, e.g., at most one, two, three,
four, five, six, seven, eight, nine, 10, 20, 30, 40, 50, 100 or 200
contiguous amino acid substitutions relative to amino acids 446-864
of SEQ ID NO: 4. In other aspects of this embodiment, a BoNT/D
H.sub.N region comprises a polypeptide having, e.g., at least one,
two, three, four, five, six, seven, eight, nine, 10, 20, 30, 40,
50, 100 or 200 contiguous amino acid substitutions relative to
amino acids 446-864 of SEQ ID NO: 4. In yet other aspects of this
embodiment, a BoNT/D H.sub.N region comprises a polypeptide having,
e.g., at most one, two, three, four, five, six, seven, eight, nine,
10, 20, 30, 40, 50, 100 or 200 contiguous amino acid deletions
relative to amino acids 446-864 of SEQ ID NO: 4. In other aspects
of this embodiment, a BoNT/D H.sub.N region comprises a polypeptide
having, e.g., at least one, two, three, four, five, six, seven,
eight, nine, 10, 20, 30, 40, 50, 100 or 200 contiguous amino acid
deletions relative to amino acids 446-864 of SEQ ID NO: 4. In still
other aspects of this embodiment, a BoNT/D H.sub.N region comprises
a polypeptide having, e.g., at most one, two, three, four, five,
six, seven, eight, nine, 10, 20, 30, 40, 50, 100 or 200 contiguous
amino acid additions relative to amino acids 446-864 of SEQ ID NO:
4. In other aspects of this embodiment, a BoNT/D H.sub.N region
comprises a polypeptide having, e.g., at least one, two, three,
four, five, six, seven, eight, nine, 10, 20, 30, 40, 50, 100 or 200
contiguous amino acid additions relative to amino acids 446-864 of
SEQ ID NO: 4.
[0105] In another embodiment, a Clostridial toxin translocation
domain comprises a BoNT/E H.sub.N region. In an aspect of this
embodiment, a BoNT/E H.sub.N region comprises amino acids 423-847
of SEQ ID NO: 5. In another aspect of this embodiment, a BoNT/E
H.sub.N region comprises a naturally occurring BoNT/E H.sub.N
region variant, such as, e.g., a H.sub.N region from a BoNT/E
isoform or a H.sub.N region from a BoNT/E subtype. In another
aspect of this embodiment, a BoNT/E H.sub.N region comprises amino
acids 423-847 of a naturally occurring BoNT/E H.sub.N region
variant of SEQ ID NO: 5, such as, e.g., amino acids 423-847 of a
BoNT/E isoform of SEQ ID NO: 5 or amino acids 423-847 of a BoNT/E
subtype of SEQ ID NO: 5. In still another aspect of this
embodiment, a BoNT/E H.sub.N region comprises a non-naturally
occurring BoNT/E H.sub.N region variant, such as, e.g., a
conservative BoNT/E H.sub.N region variant, a non-conservative
BoNT/E H.sub.N region variant, a BoNT/E chimeric H.sub.N region, an
active BoNT/E H.sub.N region fragment, or any combination thereof.
In still another aspect of this embodiment, a BoNT/E H.sub.N region
comprises amino acids 423-847 of a non-naturally occurring BoNT/E
H.sub.N region variant of SEQ ID NO: 5, such as, e.g., amino acids
423-847 of a conservative BoNT/E H.sub.N region variant of SEQ ID
NO: 5, amino acids 423-847 of a non-conservative BoNT/E H.sub.N
region variant of SEQ ID NO: 5, amino acids 423-847 of an active
BoNT/E H.sub.N region fragment of SEQ ID NO: 5, or any combination
thereof.
[0106] In other aspects of this embodiment, a BoNT/E H.sub.N region
comprises a polypeptide having, e.g., at least 70% amino acid
identity with amino acids 423-847 of SEQ ID NO: 5, at least 75%
amino acid identity with amino acids 423-847 of SEQ ID NO: 5, at
least 80% amino acid identity with amino acids 423-847 of SEQ ID
NO: 5, at least 85% amino acid identity with amino acids 423-847 of
SEQ ID NO: 5, at least 90% amino acid identity with amino acids
423-847 of SEQ ID NO: 5 or at least 95% amino acid identity with
amino acids 423-847 of SEQ ID NO: 5. In yet other aspects of this
embodiment, a BoNT/E H.sub.N region comprises a polypeptide having,
e.g., at most 70% amino acid identity with amino acids 423-847 of
SEQ ID NO: 5, at most 75% amino acid identity with amino acids
423-847 of SEQ ID NO: 5, at most 80% amino acid identity with amino
acids 423-847 of SEQ ID NO: 5, at most 85% amino acid identity with
amino acids 423-847 of SEQ ID NO: 5, at most 90% amino acid
identity with amino acids 423-847 of SEQ ID NO: 5 or at most 95%
amino acid identity with amino acids 423-847 of SEQ ID NO: 5.
[0107] In other aspects of this embodiment, a BoNT/E H.sub.N region
comprises a polypeptide having, e.g., at most one, two, three,
four, five, six, seven, eight, nine, 10, 20, 30, 40, 50, 100, or
200 non-contiguous amino acid substitutions relative to amino acids
423-847 of SEQ ID NO: 5. In other aspects of this embodiment, a
BoNT/E H.sub.N region comprises a polypeptide having, e.g., at
least one, two, three, four, five, six, seven, eight, nine, 10, 20,
30, 40, 50, 100 or 200 non-contiguous amino acid substitutions
relative to amino acids 423-847 of SEQ ID NO: 5. In yet other
aspects of this embodiment, a BoNT/E H.sub.N region comprises a
polypeptide having, e.g., at most one, two, three, four, five, six,
seven, eight, nine, 10, 20, 30, 40, 50, 100 or 200 non-contiguous
amino acid deletions relative to amino acids 423-847 of SEQ ID NO:
5. In other aspects of this embodiment, a BoNT/E H.sub.N region
comprises a polypeptide having, e.g., at least one, two, three,
four, five, six, seven, eight, nine, 10, 20, 30, 40, 50, 100 or 200
non-contiguous amino acid deletions relative to amino acids 423-847
of SEQ ID NO: 5. In still other aspects of this embodiment, a
BoNT/E H.sub.N region comprises a polypeptide having, e.g., at most
one, two, three, four, five, six, seven, eight, nine, 10, 20, 30,
40, 50, 100 or 200 non-contiguous amino acid additions relative to
amino acids 423-847 of SEQ ID NO: 5. In other aspects of this
embodiment, a BoNT/E H.sub.N region comprises a polypeptide having,
e.g., at least one, two, three, four, five, six, seven, eight,
nine, 10, 20, 30, 40, 50, 100 or 200 non-contiguous amino acid
additions relative to amino acids 423-847 of SEQ ID NO: 5.
[0108] In other aspects of this embodiment, a BoNT/E H.sub.N region
comprises a polypeptide having, e.g., at most one, two, three,
four, five, six, seven, eight, nine, 10, 20, 30, 40, 50, 100 or 200
contiguous amino acid substitutions relative to amino acids 423-847
of SEQ ID NO: 5. In other aspects of this embodiment, a BoNT/E
H.sub.N region comprises a polypeptide having, e.g., at least one,
two, three, four, five, six, seven, eight, nine, 10, 20, 30, 40,
50, 100 or 200 contiguous amino acid substitutions relative to
amino acids 423-847 of SEQ ID NO: 5. In yet other aspects of this
embodiment, a BoNT/E H.sub.N region comprises a polypeptide having,
e.g., at most one, two, three, four, five, six, seven, eight, nine,
10, 20, 30, 40, 50, 100 or 200 contiguous amino acid deletions
relative to amino acids 423-847 of SEQ ID NO: 5. In other aspects
of this embodiment, a BoNT/E H.sub.N region comprises a polypeptide
having, e.g., at least one, two, three, four, five, six, seven,
eight, nine, 10, 20, 30, 40, 50, 100 or 200 contiguous amino acid
deletions relative to amino acids 423-847 of SEQ ID NO: 5. In still
other aspects of this embodiment, a BoNT/E H.sub.N region comprises
a polypeptide having, e.g., at most one, two, three, four, five,
six, seven, eight, nine, 10, 20, 30, 40, 50, 100 or 200 contiguous
amino acid additions relative to amino acids 423-847 of SEQ ID NO:
5. In other aspects of this embodiment, a BoNT/E H.sub.N region
comprises a polypeptide having, e.g., at least one, two, three,
four, five, six, seven, eight, nine, 10, 20, 30, 40, 50, 100 or 200
contiguous amino acid additions relative to amino acids 423-847 of
SEQ ID NO: 5.
[0109] In another embodiment, a Clostridial toxin translocation
domain comprises a BoNT/F H.sub.N region. In an aspect of this
embodiment, a BoNT/F H.sub.N region comprises amino acids 440-866
of SEQ ID NO: 6. In another aspect of this embodiment, a BoNT/F
H.sub.N region comprises a naturally occurring BoNT/F H.sub.N
region variant, such as, e.g., a H.sub.N region from a BoNT/F
isoform or a H.sub.N region from a BoNT/F subtype. In another
aspect of this embodiment, a BoNT/F H.sub.N region comprises amino
acids 440-866 of a naturally occurring BoNT/F H.sub.N region
variant of SEQ ID NO: 6, such as, e.g., amino acids 440-866 of a
BoNT/F isoform of SEQ ID NO: 6 or amino acids 440-866 of a BoNT/F
subtype of SEQ ID NO: 6. In still another aspect of this
embodiment, a BoNT/F H.sub.N region comprises a non-naturally
occurring BoNT/F H.sub.N region variant, such as, e.g., a
conservative BoNT/F H.sub.N region variant, a non-conservative
BoNT/F H.sub.N region variant, a BoNT/F chimeric H.sub.N region, an
active BoNT/F H.sub.N region fragment, or any combination thereof.
In still another aspect of this embodiment, a BoNT/F H.sub.N region
comprises amino acids 440-866 of a non-naturally occurring BoNT/F
H.sub.N region variant of SEQ ID NO: 6, such as, e.g., amino acids
440-866 of a conservative BoNT/F H.sub.N region variant of SEQ ID
NO: 6, amino acids 440-866 of a non-conservative BoNT/F H.sub.N
region variant of SEQ ID NO: 6, amino acids 440-866 of an active
BoNT/F H.sub.N region fragment of SEQ ID NO: 6, or any combination
thereof.
[0110] In other aspects of this embodiment, a BoNT/F H.sub.N region
comprises a polypeptide having, e.g., at least 70% amino acid
identity with amino acids 440-866 of SEQ ID NO: 6, at least 75%
amino acid identity with amino acids 440-866 of SEQ ID NO: 6, at
least 80% amino acid identity with amino acids 440-866 of SEQ ID
NO: 6, at least 85% amino acid identity with amino acids 440-866 of
SEQ ID NO: 6, at least 90% amino acid identity with amino acids
440-866 of SEQ ID NO: 6 or at least 95% amino acid identity with
amino acids 440-866 of SEQ ID NO: 6. In yet other aspects of this
embodiment, a BoNT/F H.sub.N region comprises a polypeptide having,
e.g., at most 70% amino acid identity with amino acids 440-866 of
SEQ ID NO: 6, at most 75% amino acid identity with amino acids
440-866 of SEQ ID NO: 6, at most 80% amino acid identity with amino
acids 440-866 of SEQ ID NO: 6, at most 85% amino acid identity with
amino acids 440-866 of SEQ ID NO: 6, at most 90% amino acid
identity with amino acids 440-866 of SEQ ID NO: 6 or at most 95%
amino acid identity with amino acids 440-866 of SEQ ID NO: 6.
[0111] In other aspects of this embodiment, a BoNT/F H.sub.N region
comprises a polypeptide having, e.g., at most one, two, three,
four, five, six, seven, eight, nine, 10, 20, 30, 40, 50, 100, or
200 non-contiguous amino acid substitutions relative to amino acids
440-866 of SEQ ID NO: 6. In other aspects of this embodiment, a
BoNT/F H.sub.N region comprises a polypeptide having, e.g., at
least one, two, three, four, five, six, seven, eight, nine, 10, 20,
30, 40, 50, 100 or 200 non-contiguous amino acid substitutions
relative to amino acids 440-866 of SEQ ID NO: 6. In yet other
aspects of this embodiment, a BoNT/F H.sub.N region comprises a
polypeptide having, e.g., at most one, two, three, four, five, six,
seven, eight, nine, 10, 20, 30, 40, 50, 100 or 200 non-contiguous
amino acid deletions relative to amino acids 440-866 of SEQ ID NO:
6. In other aspects of this embodiment, a BoNT/F H.sub.N region
comprises a polypeptide having, e.g., at least one, two, three,
four, five, six, seven, eight, nine, 10, 20, 30, 40, 50, 100 or 200
non-contiguous amino acid deletions relative to amino acids 440-866
of SEQ ID NO: 6. In still other aspects of this embodiment, a
BoNT/F H.sub.N region comprises a polypeptide having, e.g., at most
one, two, three, four, five, six, seven, eight, nine, 10, 20, 30,
40, 50, 100 or 200 non-contiguous amino acid additions relative to
amino acids 440-866 of SEQ ID NO: 6. In other aspects of this
embodiment, a BoNT/F H.sub.N region comprises a polypeptide having,
e.g., at least one, two, three, four, five, six, seven, eight,
nine, 10, 20, 30, 40, 50, 100 or 200 non-contiguous amino acid
additions relative to amino acids 440-866 of SEQ ID NO: 6.
[0112] In other aspects of this embodiment, a BoNT/F H.sub.N region
comprises a polypeptide having, e.g., at most one, two, three,
four, five, six, seven, eight, nine, 10, 20, 30, 40, 50, 100 or 200
contiguous amino acid substitutions relative to amino acids 440-866
of SEQ ID NO: 6. In other aspects of this embodiment, a BoNT/F
H.sub.N region comprises a polypeptide having, e.g., at least one,
two, three, four, five, six, seven, eight, nine, 10, 20, 30, 40,
50, 100 or 200 contiguous amino acid substitutions relative to
amino acids 440-866 of SEQ ID NO: 6. In yet other aspects of this
embodiment, a BoNT/F H.sub.N region comprises a polypeptide having,
e.g., at most one, two, three, four, five, six, seven, eight, nine,
10, 20, 30, 40, 50, 100 or 200 contiguous amino acid deletions
relative to amino acids 440-866 of SEQ ID NO: 6. In other aspects
of this embodiment, a BoNT/F H.sub.N region comprises a polypeptide
having, e.g., at least one, two, three, four, five, six, seven,
eight, nine, 10, 20, 30, 40, 50, 100 or 200 contiguous amino acid
deletions relative to amino acids 440-866 of SEQ ID NO: 6. In still
other aspects of this embodiment, a BoNT/F H.sub.N region comprises
a polypeptide having, e.g., at most one, two, three, four, five,
six, seven, eight, nine, 10, 20, 30, 40, 50, 100 or 200 contiguous
amino acid additions relative to amino acids 440-866 of SEQ ID NO:
6. In other aspects of this embodiment, a BoNT/F H.sub.N region
comprises a polypeptide having, e.g., at least one, two, three,
four, five, six, seven, eight, nine, 10, 20, 30, 40, 50, 100 or 200
contiguous amino acid additions relative to amino acids 440-866 of
SEQ ID NO: 6.
[0113] In another embodiment, a Clostridial toxin translocation
domain comprises a BoNT/G H.sub.N region. In an aspect of this
embodiment, a BoNT/G H.sub.N region comprises amino acids 447-865
of SEQ ID NO: 7. In another aspect of this embodiment, a BoNT/G
H.sub.N region comprises a naturally occurring BoNT/G H.sub.N
region variant, such as, e.g., a H.sub.N region from a BoNT/G
isoform or a H.sub.N region from a BoNT/G subtype. In another
aspect of this embodiment, a BoNT/G H.sub.N region comprises amino
acids 447-865 of a naturally occurring BoNT/G H.sub.N region
variant of SEQ ID NO: 7, such as, e.g., amino acids 447-865 of a
BoNT/G isoform of SEQ ID NO: 7 or amino acids 447-865 of a BoNT/G
subtype of SEQ ID NO: 7. In still another aspect of this
embodiment, a BoNT/G H.sub.N region comprises a non-naturally
occurring BoNT/G H.sub.N region variant, such as, e.g., a
conservative BoNT/G H.sub.N region variant, a non-conservative
BoNT/G H.sub.N region variant, a BoNT/G chimeric H.sub.N region, an
active BoNT/G H.sub.N region fragment, or any combination thereof.
In still another aspect of this embodiment, a BoNT/G H.sub.N region
comprises amino acids 447-865 of a non-naturally occurring BoNT/G
H.sub.N region variant of SEQ ID NO: 7, such as, e.g., amino acids
447-865 of a conservative BoNT/G H.sub.N region variant of SEQ ID
NO: 7, amino acids 447-865 of a non-conservative BoNT/G H.sub.N
region variant of SEQ ID NO: 7, amino acids 447-865 of an active
BoNT/G H.sub.N region fragment of SEQ ID NO: 7, or any combination
thereof.
[0114] In other aspects of this embodiment, a BoNT/G H.sub.N region
comprises a polypeptide having, e.g., at least 70% amino acid
identity with amino acids 447-865 of SEQ ID NO: 7, at least 75%
amino acid identity with amino acids 447-865 of SEQ ID NO: 7, at
least 80% amino acid identity with amino acids 447-865 of SEQ ID
NO: 7, at least 85% amino acid identity with amino acids 447-865 of
SEQ ID NO: 7, at least 90% amino acid identity with amino acids
447-865 of SEQ ID NO: 7 or at least 95% amino acid identity with
amino acids 447-865 of SEQ ID NO: 7. In yet other aspects of this
embodiment, a BoNT/G H.sub.N region comprises a polypeptide having,
e.g., at most 70% amino acid identity with amino acids 447-865 of
SEQ ID NO: 7, at most 75% amino acid identity with amino acids
447-865 of SEQ ID NO: 7, at most 80% amino acid identity with amino
acids 447-865 of SEQ ID NO: 7, at most 85% amino acid identity with
amino acids 447-865 of SEQ ID NO: 7, at most 90% amino acid
identity with amino acids 447-865 of SEQ ID NO: 7 or at most 95%
amino acid identity with amino acids 447-865 of SEQ ID NO: 7.
[0115] In other aspects of this embodiment, a BoNT/G H.sub.N region
comprises a polypeptide having, e.g., at most one, two, three,
four, five, six, seven, eight, nine, 10, 20, 30, 40, 50, 100, or
200 non-contiguous amino acid substitutions relative to amino acids
447-865 of SEQ ID NO: 7. In other aspects of this embodiment, a
BoNT/G H.sub.N region comprises a polypeptide having, e.g., at
least one, two, three, four, five, six, seven, eight, nine, 10, 20,
30, 40, 50, 100 or 200 non-contiguous amino acid substitutions
relative to amino acids 447-865 of SEQ ID NO: 7. In yet other
aspects of this embodiment, a BoNT/G H.sub.N region comprises a
polypeptide having, e.g., at most one, two, three, four, five, six,
seven, eight, nine, 10, 20, 30, 40, 50, 100 or 200 non-contiguous
amino acid deletions relative to amino acids 447-865 of SEQ ID NO:
7. In other aspects of this embodiment, a BoNT/G H.sub.N region
comprises a polypeptide having, e.g., at least one, two, three,
four, five, six, seven, eight, nine, 10, 20, 30, 40, 50, 100 or 200
non-contiguous amino acid deletions relative to amino acids 447-865
of SEQ ID NO: 7. In still other aspects of this embodiment, a
BoNT/G H.sub.N region comprises a polypeptide having, e.g., at most
one, two, three, four, five, six, seven, eight, nine, 10, 20, 30,
40, 50, 100 or 200 non-contiguous amino acid additions relative to
amino acids 447-865 of SEQ ID NO: 7. In other aspects of this
embodiment, a BoNT/G H.sub.N region comprises a polypeptide having,
e.g., at least one, two, three, four, five, six, seven, eight,
nine, 10, 20, 30, 40, 50, 100 or 200 non-contiguous amino acid
additions relative to amino acids 447-865 of SEQ ID NO: 7.
[0116] In other aspects of this embodiment, a BoNT/G H.sub.N region
comprises a polypeptide having, e.g., at most one, two, three,
four, five, six, seven, eight, nine, 10, 20, 30, 40, 50, 100 or 200
contiguous amino acid substitutions relative to amino acids 447-865
of SEQ ID NO: 7. In other aspects of this embodiment, a BoNT/G
H.sub.N region comprises a polypeptide having, e.g., at least one,
two, three, four, five, six, seven, eight, nine, 10, 20, 30, 40,
50, 100 or 200 contiguous amino acid substitutions relative to
amino acids 447-865 of SEQ ID NO: 7. In yet other aspects of this
embodiment, a BoNT/G H.sub.N region comprises a polypeptide having,
e.g., at most one, two, three, four, five, six, seven, eight, nine,
10, 20, 30, 40, 50, 100 or 200 contiguous amino acid deletions
relative to amino acids 447-865 of SEQ ID NO: 7. In other aspects
of this embodiment, a BoNT/G H.sub.N region comprises a polypeptide
having, e.g., at least one, two, three, four, five, six, seven,
eight, nine, 10, 20, 30, 40, 50, 100 or 200 contiguous amino acid
deletions relative to amino acids 447-865 of SEQ ID NO: 7. In still
other aspects of this embodiment, a BoNT/G H.sub.N region comprises
a polypeptide having, e.g., at most one, two, three, four, five,
six, seven, eight, nine, 10, 20, 30, 40, 50, 100 or 200 contiguous
amino acid additions relative to amino acids 447-865 of SEQ ID NO:
7. In other aspects of this embodiment, a BoNT/G H.sub.N region
comprises a polypeptide having, e.g., at least one, two, three,
four, five, six, seven, eight, nine, 10, 20, 30, 40, 50, 100 or 200
contiguous amino acid additions relative to amino acids 447-865 of
SEQ ID NO: 7.
[0117] In another embodiment, a Clostridial toxin translocation
domain comprises a TeNT H.sub.N region. In an aspect of this
embodiment, a TeNT H.sub.N region comprises amino acids 458-881 of
SEQ ID NO: 8. In another aspect of this embodiment, a TeNT H.sub.N
region comprises a naturally occurring TeNT H.sub.N region variant,
such as, e.g., a H.sub.N region from a TeNT isoform or a H.sub.N
region from a TeNT subtype. In another aspect of this embodiment, a
TeNT H.sub.N region comprises amino acids 458-881 of a naturally
occurring TeNT H.sub.N region variant of SEQ ID NO: 8, such as,
e.g., amino acids 458-881 of a TeNT isoform of SEQ ID NO: 8 or
amino acids 458-881 of a TeNT subtype of SEQ ID NO: 8. In still
another aspect of this embodiment, a TeNT H.sub.N region comprises
a non-naturally occurring TeNT H.sub.N region variant, such as,
e.g., a conservative TeNT H.sub.N region variant, a
non-conservative TeNT H.sub.N region variant, a TeNT chimeric
H.sub.N region, an active TeNT H.sub.N region fragment, or any
combination thereof. In still another aspect of this embodiment, a
TeNT H.sub.N region comprises amino acids 458-881 of a
non-naturally occurring TeNT H.sub.N region variant of SEQ ID NO:
8, such as, e.g., amino acids 458-881 of a conservative TeNT
H.sub.N region variant of SEQ ID NO: 8, amino acids 458-881 of a
non-conservative TeNT H.sub.N region variant of SEQ ID NO: 8, amino
acids 458-881 of an active TeNT H.sub.N region fragment of SEQ ID
NO: 8, or any combination thereof.
[0118] In other aspects of this embodiment, a TeNT H.sub.N region
comprises a polypeptide having, e.g., at least 70% amino acid
identity with amino acids 458-881 of SEQ ID NO: 8, at least 75%
amino acid identity with amino acids 458-881 of SEQ ID NO: 8, at
least 80% amino acid identity with amino acids 458-881 of SEQ ID
NO: 8, at least 85% amino acid identity with amino acids 458-881 of
SEQ ID NO: 8, at least 90% amino acid identity with amino acids
458-881 of SEQ ID NO: 8 or at least 95% amino acid identity with
amino acids 458-881 of SEQ ID NO: 8. In yet other aspects of this
embodiment, a TeNT H.sub.N region comprises a polypeptide having,
e.g., at most 70% amino acid identity with amino acids 458-881 of
SEQ ID NO: 8, at most 75% amino acid identity with amino acids
458-881 of SEQ ID NO: 8, at most 80% amino acid identity with amino
acids 458-881 of SEQ ID NO: 8, at most 85% amino acid identity with
amino acids 458-881 of SEQ ID NO: 8, at most 90% amino acid
identity with amino acids 458-881 of SEQ ID NO: 8 or at most 95%
amino acid identity with amino acids 458-881 of SEQ ID NO: 8.
[0119] In other aspects of this embodiment, a TeNT H.sub.N region
comprises a polypeptide having, e.g., at most one, two, three,
four, five, six, seven, eight, nine, 10, 20, 30, 40, 50, 100, or
200 non-contiguous amino acid substitutions relative to amino acids
458-881 of SEQ ID NO: 8. In other aspects of this embodiment, a
TeNT H.sub.N region comprises a polypeptide having, e.g., at least
one, two, three, four, five, six, seven, eight, nine, 10, 20, 30,
40, 50, 100 or 200 non-contiguous amino acid substitutions relative
to amino acids 458-881 of SEQ ID NO: 8. In yet other aspects of
this embodiment, a TeNT H.sub.N region comprises a polypeptide
having, e.g., at most one, two, three, four, five, six, seven,
eight, nine, 10, 20, 30, 40, 50, 100 or 200 non-contiguous amino
acid deletions relative to amino acids 458-881 of SEQ ID NO: 8. In
other aspects of this embodiment, a TeNT H.sub.N region comprises a
polypeptide having, e.g., at least one, two, three, four, five,
six, seven, eight, nine, 10, 20, 30, 40, 50, 100 or 200
non-contiguous amino acid deletions relative to amino acids 458-881
of SEQ ID NO: 8. In still other aspects of this embodiment, a TeNT
H.sub.N region comprises a polypeptide having, e.g., at most one,
two, three, four, five, six, seven, eight, nine, 10, 20, 30, 40,
50, 100 or 200 non-contiguous amino acid additions relative to
amino acids 458-881 of SEQ ID NO: 8. In other aspects of this
embodiment, a TeNT H.sub.N region comprises a polypeptide having,
e.g., at least one, two, three, four, five, six, seven, eight,
nine, 10, 20, 30, 40, 50, 100 or 200 non-contiguous amino acid
additions relative to amino acids 458-881 of SEQ ID NO: 8.
[0120] In other aspects of this embodiment, a TeNT H.sub.N region
comprises a polypeptide having, e.g., at most one, two, three,
four, five, six, seven, eight, nine, 10, 20, 30, 40, 50, 100 or 200
contiguous amino acid substitutions relative to amino acids 458-881
of SEQ ID NO: 8. In other aspects of this embodiment, a TeNT
H.sub.N region comprises a polypeptide having, e.g., at least one,
two, three, four, five, six, seven, eight, nine, 10, 20, 30, 40,
50, 100 or 200 contiguous amino acid substitutions relative to
amino acids 458-881 of SEQ ID NO: 8. In yet other aspects of this
embodiment, a TeNT H.sub.N region comprises a polypeptide having,
e.g., at most one, two, three, four, five, six, seven, eight, nine,
10, 20, 30, 40, 50, 100 or 200 contiguous amino acid deletions
relative to amino acids 458-881 of SEQ ID NO: 8. In other aspects
of this embodiment, a TeNT H.sub.N region comprises a polypeptide
having, e.g., at least one, two, three, four, five, six, seven,
eight, nine, 10, 20, 30, 40, 50, 100 or 200 contiguous amino acid
deletions relative to amino acids 458-881 of SEQ ID NO: 8. In still
other aspects of this embodiment, a TeNT H.sub.N region comprises a
polypeptide having, e.g., at most one, two, three, four, five, six,
seven, eight, nine, 10, 20, 30, 40, 50, 100 or 200 contiguous amino
acid additions relative to amino acids 458-881 of SEQ ID NO: 8. In
other aspects of this embodiment, a TeNT H.sub.N region comprises a
polypeptide having, e.g., at least one, two, three, four, five,
six, seven, eight, nine, 10, 20, 30, 40, 50, 100 or 200 contiguous
amino acid additions relative to amino acids 458-881 of SEQ ID NO:
8.
[0121] Aspects of the present invention provide, in part, a
translocation facilitating domain. As used herein, the term
"translocation facilitating domain" means any polypeptide that can
further facilitate the translocation step of the intoxication
process that mediates Clostridial toxin light chain translocation.
Thus, a translocation facilitating domain assists the Clostridial
toxin translocation domain in the movement of a Clostridial toxin
light chain across a membrane and encompasses the movement of a
Clostridial toxin light chain through the membrane of an
intracellular vesicle into the cytoplasm of a cell. A non-limiting
example of a translocation facilitating domain is a Clostridial
toxin translocation facilitating domain, such as, e.g., a
Clostridial toxin H.sub.CN region such as, e.g., a BoNT/A H.sub.CN
region, a BoNT/B H.sub.CN region, a BoNT/C1 H.sub.CN region, a
BoNT/D H.sub.CN region, a BoNT/E H.sub.CN region, a BoNT/F H.sub.CN
region, a BoNT/G H.sub.CN region, and a TeNT H.sub.CN region.
Another non-limiting example of a translocation facilitating domain
is a viral fusogenic peptide domain found in an enveloped virus,
such as, e.g., an influenzavirus, an alphavirus, a vesiculovirus, a
respirovirus, a morbillivirus, an avulavirus, a henipavirus, a
metapneumovirus and a foamy virus.
[0122] Thus, in an embodiment, a translocation facilitating domain
facilitates the Clostridial toxin translocation domain in the
movement of a Clostridial toxin light chain across a membrane. In
aspects of this embodiment, a translocation facilitating domain
facilitates the Clostridial toxin translocation domain in the
movement of a Clostridial toxin light chain across a membrane by
increasing the amount of Clostridial toxin light chain in the
cytoplasm by, e.g., at least 10%, at least 20%, at least 30%, at
least 40%, at least 50%, at least 60%, at least 70%, at least 80%,
at least 90% or at least 100%. In other aspects of this embodiment,
a translocation facilitating domain facilitates the Clostridial
toxin translocation domain in the movement of a Clostridial toxin
light chain across a membrane by increasing the amount of
Clostridial toxin light chain in the cytoplasm by, e.g., at least
two-fold, at least three-fold, at least four-fold, at least
five-fold, at least ten-fold or at least twent.gamma.-fold. In yet
other aspects of this embodiment, a translocation facilitating
domain facilitates the Clostridial toxin translocation domain in
the movement of a Clostridial toxin light chain across a membrane
by increasing the amount of Clostridial toxin light chain in the
cytoplasm by, e.g., at most 10%, at most 20%, at most 30%, at most
40%, at most 50%, at most 60%, at most 70%, at most 80%, at most
90% or at most 100%. In other aspects of this embodiment, a
translocation facilitating domain facilitates the Clostridial toxin
translocation domain in the movement of a Clostridial toxin light
chain across a membrane by increasing the amount of Clostridial
toxin light chain in the cytoplasm by, e.g., at most two-fold, at
most three-fold, at most four-fold, at most five-fold, at most
ten-fold or at most twent.gamma.-fold.
[0123] A Clostridial toxin "translocation facilitating domain
includes, without limitation, naturally occurring Clostridial toxin
H.sub.CN region variants, such as, e.g., Clostridial toxin H.sub.CN
region isoforms and Clostridial toxin H.sub.CN region subtypes;
non-naturally occurring Clostridial toxin H.sub.CN region variants,
such as, e.g., conservative Clostridial toxin H.sub.CN region
variants, non-conservative Clostridial toxin H.sub.CN region
variants, Clostridial toxin H.sub.CN region chimerics, active
Clostridial toxin H.sub.CN region fragments thereof, or any
combination thereof.
[0124] As used herein, the term "Clostridial toxin H.sub.CN region
variant," whether naturally-occurring or non-naturally-occurring,
means a Clostridial toxin H.sub.CN region that has at least one
amino acid change from the corresponding region of the disclosed
reference sequences (see Table 1) and can be described in percent
identity to the corresponding region of that reference sequence.
Unless expressly indicated, all Clostridial toxin H.sub.CN region
variants disclosed in the present specification are capable of
further facilitating the translocation step of the intoxication
process that mediates Clostridial toxin light chain translocation.
As non-limiting examples, a BoNT/A H.sub.CN region variant
comprising amino acids 874-1110 of SEQ ID NO: 1 will have at least
one amino acid difference, such as, e.g., an amino acid
substitution, deletion or addition, as compared to the amino acid
region 874-1110 of SEQ ID NO: 1; a BoNT/B H.sub.CN region variant
comprising amino acids 861-1097 of SEQ ID NO: 2 will have at least
one amino acid difference, such as, e.g., an amino acid
substitution, deletion or addition, as compared to the amino acid
region 861-1097 of SEQ ID NO: 2; a BoNT/C1 H.sub.CN region variant
comprising amino acids 869-1111 of SEQ ID NO: 3 will have at least
one amino acid difference, such as, e.g., an amino acid
substitution, deletion or addition, as compared to the amino acid
region 869-1111 of SEQ ID NO: 3; a BoNT/D H.sub.CN region variant
comprising amino acids 865-1098 of SEQ ID NO: 4 will have at least
one amino acid difference, such as, e.g., an amino acid
substitution, deletion or addition, as compared to the amino acid
region 865-1098 of SEQ ID NO: 4; a BoNT/E H.sub.CN region variant
comprising amino acids 848-1085 of SEQ ID NO: 5 will have at least
one amino acid difference, such as, e.g., an amino acid
substitution, deletion or addition, as compared to the amino acid
region 848-1085 of SEQ ID NO: 5; a BoNT/F H.sub.CN region variant
comprising amino acids 867-1105 of SEQ ID NO: 6 will have at least
one amino acid difference, such as, e.g., an amino acid
substitution, deletion or addition, as compared to the amino acid
region 867-1105 of SEQ ID NO: 6; a BoNT/G H.sub.CN region variant
comprising amino acids 866-1105 of SEQ ID NO: 7 will have at least
one amino acid difference, such as, e.g., an amino acid
substitution, deletion or addition, as compared to the amino acid
region 866-1105 of SEQ ID NO: 7; and a TeNT H.sub.CN region variant
comprising amino acids 882-1127 of SEQ ID NO: 8 will have at least
one amino acid difference, such as, e.g., an amino acid
substitution, deletion or addition, as compared to the amino acid
region 882-1127 of SEQ ID NO: 8.
[0125] It is recognized by those of skill in the art that within
each serotype of Clostridial toxin there can be naturally occurring
Clostridial toxin H.sub.CN region variants that differ somewhat in
their amino acid sequence, and also in the nucleic acids encoding
these proteins. For example, there are presently four BoNT/A
subtypes, BoNT/A1, BoNT/A2, BoNT/A3 and BoNT/A4, with specific
H.sub.CN region subtypes showing approximately 87% amino acid
identity when compared to another BoNT/A H.sub.CN region subtype.
As used herein, the term "naturally occurring Clostridial toxin
H.sub.CN region variant" means any Clostridial toxin H.sub.CN
region produced by a naturally-occurring process, including,
without limitation, Clostridial toxin H.sub.CN region isoforms
produced from alternatively-spliced transcripts, Clostridial toxin
H.sub.CN region isoforms produced by spontaneous mutation and
Clostridial toxin H.sub.CN region subtypes. A naturally occurring
Clostridial toxin H.sub.CN region variant can function in
substantially the same manner as the reference Clostridial toxin
H.sub.CN region on which the naturally occurring Clostridial toxin
H.sub.CN region variant is based, and can be substituted for the
reference Clostridial toxin H.sub.CN region in any aspect of the
present invention. A naturally occurring Clostridial toxin H.sub.CN
region variant may substitute one or more amino acids, two or more
amino acids, three or more amino acids, four or more amino acids,
five or more amino acids, ten or more amino acids, 20 or more amino
acids, 30 or more amino acids, 40 or more amino acids or 50 or more
amino acids from the reference Clostridial toxin H.sub.CN region on
which the naturally occurring Clostridial toxin H.sub.CN region
variant is based. A naturally occurring Clostridial toxin H.sub.CN
region variant can also substitute at least 10 contiguous amino
acids, at least 15 contiguous amino acids, at least 20 contiguous
amino acids, or at least 25 contiguous amino acids from the
reference Clostridial toxin H.sub.CN region on which the naturally
occurring Clostridial toxin H.sub.CN region variant is based, that
possess at least 50% amino acid identity, 65% amino acid identity,
75% amino acid identity, 85% amino acid identity or 95% amino acid
identity to the reference Clostridial toxin H.sub.CN region on
which the naturally occurring Clostridial toxin H.sub.CN region
variant is based.
[0126] A non-limiting examples of a naturally occurring Clostridial
toxin H.sub.CN region variant is a Clostridial toxin H.sub.CN
region isoform such as, e.g., a BoNT/A H.sub.CN region isoform, a
BoNT/B H.sub.CN region isoform, a BoNT/C1 H.sub.CN region isoform,
a BoNT/D H.sub.CN region isoform, a BoNT/E H.sub.CN region isoform,
a BoNT/F H.sub.CN region isoform, a BoNT/G H.sub.CN region isoform,
and a TeNT H.sub.CN region isoform. A Clostridial toxin H.sub.CN
region isoform can function in substantially the same manner as the
reference Clostridial toxin H.sub.CN region on which the
Clostridial toxin H.sub.CN region isoform is based, and can be
substituted for the reference Clostridial toxin H.sub.CN region in
any aspect of the present invention.
[0127] Another non-limiting examples of a naturally occurring
Clostridial toxin H.sub.CN region variant is a Clostridial toxin
H.sub.CN region subtype such as, e.g., a H.sub.CN region from
subtype BoNT/A1, BoNT/A2, BoNT/A3 and BoNT/A4; a H.sub.CN region
from subtype BoNT/B1, BoNT/B2, BoNT/B bivalent and BoNT/B
nonproteolytic; a H.sub.CN region from subtype BoNT/C1-1 and
BoNT/C1-2; a H.sub.CN region from subtype BoNT/E1, BoNT/E2 and
BoNT/E3; and a H.sub.CN region from subtype BoNT/F1, BoNT/F2,
BoNT/F3 and BoNT/F4. A Clostridial toxin H.sub.CN region subtype
can function in substantially the same manner as the reference
Clostridial toxin H.sub.CN region on which the Clostridial toxin
H.sub.CN region subtype is based, and can be substituted for the
reference Clostridial toxin H.sub.CN region in any aspect of the
present invention.
[0128] As used herein, the term "non-naturally occurring
Clostridial toxin H.sub.CN region variant" means any Clostridial
toxin H.sub.CN region produced with the aid of human manipulation,
including, without limitation, Clostridial toxin H.sub.CN regions
produced by genetic engineering using random mutagenesis or
rational design and Clostridial toxin H.sub.CN regions produced by
chemical synthesis. Non-limiting examples of non-naturally
occurring Clostridial toxin H.sub.CN region variants include, e.g.,
conservative Clostridial toxin H.sub.CN region variants,
non-conservative Clostridial toxin H.sub.CN region variants,
Clostridial toxin H.sub.CN region chimeric variants and active
Clostridial toxin H.sub.CN region fragments.
[0129] As used herein, the term "conservative Clostridial toxin
H.sub.CN region variant" means a Clostridial toxin H.sub.CN region
that has at least one amino acid substituted by another amino acid
or an amino acid analog that has at least one property similar to
that of the original amino acid from the reference Clostridial
toxin H.sub.CN region sequence (Table 1). Examples of properties
include, without limitation, similar size, topography, charge,
hydrophobicity, hydrophilicity, lipophilicity, covalent-bonding
capacity, hydrogen-bonding capacity, a physicochemical property, of
the like, or any combination thereof. A conservative Clostridial
toxin H.sub.CN region variant can function in substantially the
same manner as the reference Clostridial toxin H.sub.CN region on
which the conservative Clostridial toxin H.sub.CN region variant is
based, and can be substituted for the reference Clostridial toxin
H.sub.CN region in any aspect of the present invention. A
conservative Clostridial toxin H.sub.CN region variant may
substitute one or more amino acids, two or more amino acids, three
or more amino acids, four or more amino acids, five or more amino
acids, ten or more amino acids, 20 or more amino acids, 30 or more
amino acids, 40 or more amino acids, 50 or more amino acids or 100
or more amino acids from the reference Clostridial toxin H.sub.CN
region on which the conservative Clostridial toxin H.sub.CN region
variant is based. A conservative Clostridial toxin H.sub.CN region
variant can also substitute at least 10 contiguous amino acids, at
least 15 contiguous amino acids, at least 20 contiguous amino
acids, or at least 25 contiguous amino acids from the reference
Clostridial toxin H.sub.CN region on which the conservative
Clostridial toxin H.sub.CN region variant is based, that possess at
least 50% amino acid identity, 65% amino acid identity, 75% amino
acid identity, 85% amino acid identity or 95% amino acid identity
to the reference Clostridial toxin H.sub.CN region on which the
conservative Clostridial toxin H.sub.CN region variant is based.
Non-limiting examples of a conservative Clostridial toxin H.sub.CN
region variant include, e.g., conservative BoNT/A H.sub.CN region
variants, conservative BoNT/B H.sub.CN region variants,
conservative BoNT/C1 H.sub.CN region variants, conservative BoNT/D
H.sub.CN region variants, conservative BoNT/E H.sub.CN region
variants, conservative BoNT/F H.sub.CN region variants,
conservative BoNT/G H.sub.CN region variants, and conservative TeNT
H.sub.CN region variants.
[0130] As used herein, the term "non-conservative Clostridial toxin
H.sub.CN region variant" means a Clostridial toxin H.sub.CN region
in which 1) at least one amino acid is deleted from the reference
Clostridial toxin H.sub.CN region on which the non-conservative
Clostridial toxin H.sub.CN region variant is based; 2) at least one
amino acid added to the reference Clostridial toxin H.sub.CN region
on which the non-conservative Clostridial toxin H.sub.CN region is
based; or 3) at least one amino acid is substituted by another
amino acid or an amino acid analog that does not share any property
similar to that of the original amino acid from the reference
Clostridial toxin H.sub.CN region sequence (Table 1). A
non-conservative Clostridial toxin H.sub.CN region variant can
function in substantially the same manner as the reference
Clostridial toxin H.sub.CN region on which the non-conservative
Clostridial toxin H.sub.CN region variant is based, and can be
substituted for the reference Clostridial toxin H.sub.CN region in
any aspect of the present invention. A non-conservative Clostridial
toxin H.sub.CN region variant can delete one or more amino acids,
two or more amino acids, three or more amino acids, four or more
amino acids, five or more amino acids, and ten or more amino acids
from the reference Clostridial toxin H.sub.CN region on which the
non-conservative Clostridial toxin H.sub.CN region variant is
based. A non-conservative Clostridial toxin H.sub.CN region variant
can add one or more amino acids, two or more amino acids, three or
more amino acids, four or more amino acids, five or more amino
acids, and ten or more amino acids to the reference Clostridial
toxin H.sub.CN region on which the non-conservative Clostridial
toxin H.sub.CN region variant is based. A non-conservative
Clostridial toxin H.sub.CN region variant may substitute one or
more amino acids, two or more amino acids, three or more amino
acids, four or more amino acids, five or more amino acids, ten or
more amino acids, 20 or more amino acids, 30 or more amino acids,
40 or more amino acids, 50 or more amino acids or 100 or more amino
acids from the reference Clostridial toxin H.sub.CN region on which
the non-conservative Clostridial toxin H.sub.CN region variant is
based. A non-conservative Clostridial toxin H.sub.CN region variant
can also substitute at least 10 contiguous amino acids, at least 15
contiguous amino acids, at least 20 contiguous amino acids, or at
least 25 contiguous amino acids from the reference Clostridial
toxin H.sub.CN region on which the non-conservative Clostridial
toxin H.sub.CN region variant is based, that possess at least 50%
amino acid identity, 65% amino acid identity, 75% amino acid
identity, 85% amino acid identity or 95% amino acid identity to the
reference Clostridial toxin H.sub.CN region on which the
non-conservative Clostridial toxin H.sub.CN region variant is
based. Non-limiting examples of a non-conservative Clostridial
toxin H.sub.CN region variant include, e.g., non-conservative
BoNT/A H.sub.CN region variants, non-conservative BoNT/B H.sub.CN
region variants, non-conservative BoNT/C1 H.sub.CN region variants,
non-conservative BoNT/D H.sub.CN region variants, non-conservative
BoNT/E H.sub.CN region variants, non-conservative BoNT/F H.sub.CN
region variants, non-conservative BoNT/G H.sub.CN region variants,
and non-conservative TeNT H.sub.CN region variants.
[0131] As used herein, the term "Clostridial toxin H.sub.CN region
chimeric" means a polypeptide comprising at least a portion of a
Clostridial toxin H.sub.CN region and at least a portion of at
least one other polypeptide to form a toxin H.sub.CN region with at
least one property different from the reference Clostridial toxin
H.sub.CN regions of Table 1, with the proviso that this Clostridial
toxin H.sub.CN region chimeric is still capable of further
facilitating the translocation step of the intoxication process
where the LC is released from intracellular vesicles into the
cytoplasm of the target cell and thus participate in executing the
overall cellular mechanism whereby a Clostridial toxin
proteolytically cleaves a substrate.
[0132] As used herein, the term "active Clostridial toxin H.sub.CN
region fragment" means any of a variety of Clostridial toxin
fragments comprising the H.sub.CN region can be useful in aspects
of the present invention with the proviso that these active
fragments can further facilitate the translocation step of the
intoxication process where the LC is released from intracellular
vesicles into the cytoplasm of the target cell and thus participate
in executing the overall cellular mechanism whereby a Clostridial
toxin proteolytically cleaves a substrate. The H.sub.CN regions
from the heavy chains of Clostridial toxins are approximately
230-250 amino acids in length and comprise a translocation domain
(Table 1). Additionally, while a specific amino acid positions have
been identified to delineate the boundaries of the Clostridial
toxin H.sub.CN region (Table 1), it is well known in the art that
the functional boundaries are not definitive. For example,
amino-terminus of the H.sub.CN domain for all naturally-occurring
Clostridial toxins is the H.sub.N domain (translocation domain). In
examining the structure for BoNT/A, a random coil linker region
forms a boundary between the H.sub.N and H.sub.CN domains (see FIG.
7A). The BoNT/A H.sub.N domain appears to end with an .alpha.-helix
comprising amino acids N859 to 1873. Following the .alpha.-helix
there is a random coil (1873 to 1878) that leads into the H.sub.CN
domain where a .beta.-sheet begins at position 1878. The above
residues define boundaries at the beginning or end of defined
secondary structures and do not imply that there are not
significant interactions (i.e., hydrophobic, H-bond, etc.) between
residues in the random coil region and one or both of the domains
that it links together. Thus, minimally an amino acid that defines
the amino-terminal boundary of the BoNT/A H.sub.CN domain comprises
can be any amino acid present in the amino acid region Y869 to
L879. Similar analysis indicates that minimally, the amino acid
that defines the amino-terminal boundary of the BoNT/B H.sub.CN
domain can be any amino acid present in the amino acid region Y856
to L866; the amino acid that defines the amino-terminal boundary of
the BoNT/C1 H.sub.CN domain can be any amino acid present in the
amino acid region Y864 to L874; the amino acid that defines the
amino-terminal boundary of the BoNT/D H.sub.CN domain can be any
amino acid present in the amino acid region Y860 to L870; the amino
acid that defines the amino-terminal boundary of the BoNT/E
H.sub.CN domain can be any amino acid present in the amino acid
region F843 to L853; the amino acid that defines the amino-terminal
boundary of the BoNT/F H.sub.CN domain can be any amino acid
present in the amino acid region L862 to L872; the amino acid that
defines the amino-terminal boundary of the BoNT/G H.sub.CN domain
can be any amino acid present in the amino acid region Y861 to
L871; and the amino acid that defines the amino-terminal boundary
of the TeNT H.sub.CN domain can be any amino acid present in the
amino acid region 1877 to L887.
[0133] Similarly, the carboxyl-terminal portion of the H.sub.CN
domain (i.e., the fusion point between the H.sub.CN and H.sub.CC
domains) all naturally-occurring Clostridial toxins comprises a
range of amino acids. In defining the boundary of the BoNT/A
H.sub.CN domain as the beginning or the end of ordered secondary
structure, the H.sub.CN domain could end at Q1091 of an
.alpha.-helix and the H.sub.CC domain could begin at K1109 of a
.beta.-strand (see FIG. 7B). The intervening amino acid sequence
between these two domains comprises a longer random coil but, this
does not imply that the random coil is not structurally important.
In fact, this random coil has a great deal of interaction with both
the H.sub.CN and H.sub.CC domains (i.e., hydrophobic and
H-bonding). Thus, minimally an amino acid that defines the
carboxyl-terminal boundary of the BoNT/A H.sub.CN domain comprises
can be any amino acid present in the amino acid region D1089 to
Y1111. Similar analysis indicates that minimally, the amino acid
that defines the carboxyl-terminal boundary of the BoNT/B H.sub.CN
domain can be any amino acid present in the amino acid region K1076
to Y1098; the amino acid that defines the carboxyl-terminal
boundary of the BoNT/C1 H.sub.CN domain can be any amino acid
present in the amino acid region N1090 to Y1112; the amino acid
that defines the carboxyl-terminal boundary of the BoNT/D H.sub.CN
domain can be any amino acid present in the amino acid region E1077
to Y1099; the amino acid that defines the carboxyl-terminal
boundary of the BoNT/E H.sub.CN domain can be any amino acid
present in the amino acid region S1064 to Y1086; the amino acid
that defines the carboxyl-terminal boundary of the BoNT/F H.sub.CN
domain can be any amino acid present in the amino acid region S1084
to Y1106; the amino acid that defines the carboxyl-terminal
boundary of the BoNT/G H.sub.CN domain can be any amino acid
present in the amino acid region W1084 to Y1106; and the amino acid
that defines the carboxyl-terminal boundary of the TeNT H.sub.CN
domain can be any amino acid present in the amino acid region T1106
to Y1128.
[0134] Thus, aspects of this embodiment can include Clostridial
toxin H.sub.CN regions comprising a translocation facilitating
domain having a length of, e.g., at least 200 amino acids, at least
225 amino acids, at least 250 amino acids and at least 275 amino
acids. Other aspects of this embodiment can include Clostridial
toxin H.sub.CN regions comprising translocation facilitating domain
having a length of, e.g., at most 200 amino acids, at most 225
amino acids, at most 250 amino acids and at most 275 amino
acids.
[0135] Any of a variety of sequence alignment methods can be used
to determine percent identity of naturally-occurring Clostridial
toxin H.sub.CN region variants and non-naturally-occurring
Clostridial toxin H.sub.CN region variants, including, without
limitation, global methods, local methods and hybrid methods, such
as, e.g., segment approach methods. Protocols to determine percent
identity are routine procedures within the scope of one skilled in
the art and from the teaching herein.
[0136] Thus, in an embodiment, a modified Clostridial toxin
disclosed in the present specification comprises a Clostridial
toxin translocation domain. In an aspect of this embodiment, a
Clostridial toxin translocation domain comprises a naturally
occurring Clostridial toxin H.sub.CN region variant, such as, e.g.,
a Clostridial toxin H.sub.CN region isoform or a Clostridial toxin
H.sub.CN region subtype. In another aspect of this embodiment, a
Clostridial toxin translocation domain comprises a non-naturally
occurring Clostridial toxin H.sub.CN region variant, such as, e.g.,
a conservative Clostridial toxin H.sub.CN region variant, a
non-conservative Clostridial toxin H.sub.CN region variant, a
Clostridial toxin chimeric H.sub.CN region, an active Clostridial
toxin H.sub.CN region fragment, or any combination thereof.
[0137] In another embodiment, a Clostridial toxin translocation
domain comprises a BoNT/A H.sub.CN region. In an aspect of this
embodiment, a BoNT/A H.sub.CN region comprises amino acids 874-1110
of SEQ ID NO: 1. In another aspect of this embodiment, a BoNT/A
H.sub.CN region comprises a naturally occurring BoNT/A H.sub.CN
region variant, such as, e.g., a H.sub.CN region from a BoNT/A
isoform or a H.sub.CN region from a BoNT/A subtype. In another
aspect of this embodiment, a BoNT/A H.sub.CN region comprises amino
acids 874-1110 of a naturally occurring BoNT/A H.sub.CN region
variant of SEQ ID NO: 1, such as, e.g., amino acids 874-1110 of a
BoNT/A isoform of SEQ ID NO: 1 or amino acids 874-1110 of a BoNT/A
subtype of SEQ ID NO: 1. In still another aspect of this
embodiment, a BoNT/A H.sub.CN region comprises a non-naturally
occurring BoNT/A H.sub.CN region variant, such as, e.g., a
conservative BoNT/A H.sub.CN region variant, a non-conservative
BoNT/A H.sub.CN region variant, a BoNT/A chimeric H.sub.CN region,
an active BoNT/A H.sub.CN region fragment, or any combination
thereof. In still another aspect of this embodiment, a BoNT/A
H.sub.CN region comprises amino acids 874-1110 of a non-naturally
occurring BoNT/A H.sub.CN region variant of SEQ ID NO: 1, such as,
e.g., amino acids 874-1110 of a conservative BoNT/A H.sub.CN region
variant of SEQ ID NO: 1, amino acids 874-1110 of a non-conservative
BoNT/A H.sub.CN region variant of SEQ ID NO: 1, amino acids
874-1110 of an active BoNT/A H.sub.CN region fragment of SEQ ID NO:
1, or any combination thereof.
[0138] In other aspects of this embodiment, a BoNT/A H.sub.CN
region comprises a polypeptide having, e.g., at least 70% amino
acid identity with amino acids 874-1110 of SEQ ID NO: 1, at least
75% amino acid identity with amino acids 874-1110 of SEQ ID NO: 1,
at least 80% amino acid identity with amino acids 874-1110 of SEQ
ID NO: 1, at least 85% amino acid identity with amino acids
874-1110 of SEQ ID NO: 1, at least 90% amino acid identity with
amino acids 874-1110 of SEQ ID NO: 1 or at least 95% amino acid
identity with amino acids 874-1110 of SEQ ID NO: 1. In yet other
aspects of this embodiment, a BoNT/A H.sub.CN region comprises a
polypeptide having, e.g., at most 70% amino acid identity with
amino acids 874-1110 of SEQ ID NO: 1, at most 75% amino acid
identity with amino acids 874-1110 of SEQ ID NO: 1, at most 80%
amino acid identity with amino acids 874-1110 of SEQ ID NO: 1, at
most 85% amino acid identity with amino acids 874-1110 of SEQ ID
NO: 1, at most 90% amino acid identity with amino acids 874-1110 of
SEQ ID NO: 1 or at most 95% amino acid identity with amino acids
874-1110 of SEQ ID NO: 1.
[0139] In other aspects of this embodiment, a BoNT/A H.sub.CN
region comprises a polypeptide having, e.g., at most one, two,
three, four, five, six, seven, eight, nine, 10, 20, 30, 40, 50,
100, or 200 non-contiguous amino acid substitutions relative to
amino acids 874-1110 of SEQ ID NO: 1. In other aspects of this
embodiment, a BoNT/A H.sub.CN region comprises a polypeptide
having, e.g., at least one, two, three, four, five, six, seven,
eight, nine, 10, 20, 30, 40, 50 or 100 non-contiguous amino acid
substitutions relative to amino acids 874-1110 of SEQ ID NO: 1. In
yet other aspects of this embodiment, a BoNT/A H.sub.CN region
comprises a polypeptide having, e.g., at most one, two, three,
four, five, six, seven, eight, nine, 10, 20, 30, 40, 50 or 100
non-contiguous amino acid deletions relative to amino acids
874-1110 of SEQ ID NO: 1. In other aspects of this embodiment, a
BoNT/A H.sub.CN region comprises a polypeptide having, e.g., at
least one, two, three, four, five, six, seven, eight, nine, 10, 20,
30, 40, 50 or 100 non-contiguous amino acid deletions relative to
amino acids 874-1110 of SEQ ID NO: 1. In still other aspects of
this embodiment, a BoNT/A H.sub.CN region comprises a polypeptide
having, e.g., at most one, two, three, four, five, six, seven,
eight, nine, 10, 20, 30, 40, 50 or 100 non-contiguous amino acid
additions relative to amino acids 874-1110 of SEQ ID NO: 1. In
other aspects of this embodiment, a BoNT/A H.sub.CN region
comprises a polypeptide having, e.g., at least one, two, three,
four, five, six, seven, eight, nine, 10, 20, 30, 40, 50 or 100
non-contiguous amino acid additions relative to amino acids
874-1110 of SEQ ID NO: 1.
[0140] In other aspects of this embodiment, a BoNT/A H.sub.CN
region comprises a polypeptide having, e.g., at most one, two,
three, four, five, six, seven, eight, nine, 10, 20, 30, 40, 50 or
100 contiguous amino acid substitutions relative to amino acids
874-1110 of SEQ ID NO: 1. In other aspects of this embodiment, a
BoNT/A H.sub.CN region comprises a polypeptide having, e.g., at
least one, two, three, four, five, six, seven, eight, nine, 10, 20,
30, 40, 50 or 100 contiguous amino acid substitutions relative to
amino acids 874-1110 of SEQ ID NO: 1. In yet other aspects of this
embodiment, a BoNT/A H.sub.CN region comprises a polypeptide
having, e.g., at most one, two, three, four, five, six, seven,
eight, nine, 10, 20, 30, 40, 50 or 100 contiguous amino acid
deletions relative to amino acids 874-1110 of SEQ ID NO: 1. In
other aspects of this embodiment, a BoNT/A H.sub.CN region
comprises a polypeptide having, e.g., at least one, two, three,
four, five, six, seven, eight, nine, 10, 20, 30, 40, 50 or 100
contiguous amino acid deletions relative to amino acids 874-1110 of
SEQ ID NO: 1. In still other aspects of this embodiment, a BoNT/A
H.sub.CN region comprises a polypeptide having, e.g., at most one,
two, three, four, five, six, seven, eight, nine, 10, 20, 30, 40, 50
or 100 contiguous amino acid additions relative to amino acids
874-1110 of SEQ ID NO: 1. In other aspects of this embodiment, a
BoNT/A H.sub.CN region comprises a polypeptide having, e.g., at
least one, two, three, four, five, six, seven, eight, nine, 10, 20,
30, 40, 50 or 100 contiguous amino acid additions relative to amino
acids 874-1110 of SEQ ID NO: 1.
[0141] In another embodiment, a Clostridial toxin translocation
domain comprises a BoNT/B H.sub.CN region. In an aspect of this
embodiment, a BoNT/B H.sub.CN region comprises amino acids 861-1097
of SEQ ID NO: 2. In another aspect of this embodiment, a BoNT/B
H.sub.CN region comprises a naturally occurring BoNT/B H.sub.CN
region variant, such as, e.g., a H.sub.CN region from a BoNT/B
isoform or a H.sub.CN region from a BoNT/B subtype. In another
aspect of this embodiment, a BoNT/B H.sub.CN region comprises amino
acids 861-1097 of a naturally occurring BoNT/B H.sub.CN region
variant of SEQ ID NO: 2, such as, e.g., amino acids 861-1097 of a
BoNT/B isoform of SEQ ID NO: 2 or amino acids 861-1097 of a BoNT/B
subtype of SEQ ID NO: 2. In still another aspect of this
embodiment, a BoNT/B H.sub.CN region comprises a non-naturally
occurring BoNT/B H.sub.CN region variant, such as, e.g., a
conservative BoNT/B H.sub.CN region variant, a non-conservative
BoNT/B H.sub.CN region variant, a BoNT/B chimeric H.sub.CN region,
an active BoNT/B H.sub.CN region fragment, or any combination
thereof. In still another aspect of this embodiment, a BoNT/B
H.sub.CN region comprises amino acids 861-1097 of a non-naturally
occurring BoNT/B H.sub.CN region variant of SEQ ID NO: 2, such as,
e.g., amino acids 861-1097 of a conservative BoNT/B H.sub.CN region
variant of SEQ ID NO: 2, amino acids 861-1097 of a non-conservative
BoNT/B H.sub.CN region variant of SEQ ID NO: 2, amino acids
861-1097 of an active BoNT/B H.sub.CN region fragment of SEQ ID NO:
2, or any combination thereof.
[0142] In other aspects of this embodiment, a BoNT/B H.sub.CN
region comprises a polypeptide having, e.g., at least 70% amino
acid identity with amino acids 861-1097 of SEQ ID NO: 2, at least
75% amino acid identity with amino acids 861-1097 of SEQ ID NO: 2,
at least 80% amino acid identity with amino acids 861-1097 of SEQ
ID NO: 2, at least 85% amino acid identity with amino acids
861-1097 of SEQ ID NO: 2, at least 90% amino acid identity with
amino acids 861-1097 of SEQ ID NO: 2 or at least 95% amino acid
identity with amino acids 861-1097 of SEQ ID NO: 2. In yet other
aspects of this embodiment, a BoNT/B H.sub.CN region comprises a
polypeptide having, e.g., at most 70% amino acid identity with
amino acids 861-1097 of SEQ ID NO: 2, at most 75% amino acid
identity with amino acids 861-1097 of SEQ ID NO: 2, at most 80%
amino acid identity with amino acids 861-1097 of SEQ ID NO: 2, at
most 85% amino acid identity with amino acids 861-1097 of SEQ ID
NO: 2, at most 90% amino acid identity with amino acids 861-1097 of
SEQ ID NO: 2 or at most 95% amino acid identity with amino acids
861-1097 of SEQ ID NO: 2.
[0143] In other aspects of this embodiment, a BoNT/B H.sub.CN
region comprises a polypeptide having, e.g., at most one, two,
three, four, five, six, seven, eight, nine, 10, 20, 30, 40, 50 or
100 non-contiguous amino acid substitutions relative to amino acids
861-1097 of SEQ ID NO: 2. In other aspects of this embodiment, a
BoNT/B H.sub.CN region comprises a polypeptide having, e.g., at
least one, two, three, four, five, six, seven, eight, nine, 10, 20,
30, 40, 50 or 100 non-contiguous amino acid substitutions relative
to amino acids 861-1097 of SEQ ID NO: 2. In yet other aspects of
this embodiment, a BoNT/B H.sub.CN region comprises a polypeptide
having, e.g., at most one, two, three, four, five, six, seven,
eight, nine, 10, 20, 30, 40, 50 or 100 non-contiguous amino acid
deletions relative to amino acids 861-1097 of SEQ ID NO: 2. In
other aspects of this embodiment, a BoNT/B H.sub.CN region
comprises a polypeptide having, e.g., at least one, two, three,
four, five, six, seven, eight, nine, 10, 20, 30, 40, 50 or 100
non-contiguous amino acid deletions relative to amino acids
861-1097 of SEQ ID NO: 2. In still other aspects of this
embodiment, a BoNT/B H.sub.CN region comprises a polypeptide
having, e.g., at most one, two, three, four, five, six, seven,
eight, nine, 10, 20, 30, 40, 50 or 100 non-contiguous amino acid
additions relative to amino acids 861-1097 of SEQ ID NO: 2. In
other aspects of this embodiment, a BoNT/B H.sub.CN region
comprises a polypeptide having, e.g., at least one, two, three,
four, five, six, seven, eight, nine, 10, 20, 30, 40, 50 or 100
non-contiguous amino acid additions relative to amino acids
861-1097 of SEQ ID NO: 2.
[0144] In other aspects of this embodiment, a BoNT/B H.sub.CN
region comprises a polypeptide having, e.g., at most one, two,
three, four, five, six, seven, eight, nine, 10, 20, 30, 40, 50 or
100 contiguous amino acid substitutions relative to amino acids
861-1097 of SEQ ID NO: 2. In other aspects of this embodiment, a
BoNT/B H.sub.CN region comprises a polypeptide having, e.g., at
least one, two, three, four, five, six, seven, eight, nine, 10, 20,
30, 40, 50 or 100 contiguous amino acid substitutions relative to
amino acids 861-1097 of SEQ ID NO: 2. In yet other aspects of this
embodiment, a BoNT/B H.sub.CN region comprises a polypeptide
having, e.g., at most one, two, three, four, five, six, seven,
eight, nine, 10, 20, 30, 40, 50 or 100 contiguous amino acid
deletions relative to amino acids 861-1097 of SEQ ID NO: 2. In
other aspects of this embodiment, a BoNT/B H.sub.CN region
comprises a polypeptide having, e.g., at least one, two, three,
four, five, six, seven, eight, nine, 10, 20, 30, 40, 50 or 100
contiguous amino acid deletions relative to amino acids 861-1097 of
SEQ ID NO: 2. In still other aspects of this embodiment, a BoNT/B
H.sub.CN region comprises a polypeptide having, e.g., at most one,
two, three, four, five, six, seven, eight, nine, 10, 20, 30, 40, 50
or 100 contiguous amino acid additions relative to amino acids
861-1097 of SEQ ID NO: 2. In other aspects of this embodiment, a
BoNT/B H.sub.CN region comprises a polypeptide having, e.g., at
least one, two, three, four, five, six, seven, eight, nine, 10, 20,
30, 40, 50 or 100 contiguous amino acid additions relative to amino
acids 861-1097 of SEQ ID NO: 2.
[0145] In another embodiment, a Clostridial toxin translocation
domain comprises a BoNT/C1 H.sub.CN region. In an aspect of this
embodiment, a BoNT/C1 H.sub.CN region comprises amino acids
869-1111 of SEQ ID NO: 3. In another aspect of this embodiment, a
BoNT/C1 H.sub.CN region comprises a naturally occurring BoNT/C1
H.sub.CN region variant, such as, e.g., a H.sub.CN region from a
BoNT/C1 isoform or a H.sub.CN region from a BoNT/C1 subtype. In
another aspect of this embodiment, a BoNT/C1 H.sub.CN region
comprises amino acids 869-1111 of a naturally occurring BoNT/C1
H.sub.CN region variant of SEQ ID NO: 3, such as, e.g., amino acids
869-1111 of a BoNT/C1 isoform of SEQ ID NO: 3 or amino acids
869-1111 of a BoNT/C1 subtype of SEQ ID NO: 3. In still another
aspect of this embodiment, a BoNT/C1 H.sub.CN region comprises a
non-naturally occurring BoNT/C1 H.sub.CN region variant, such as,
e.g., a conservative BoNT/C1 H.sub.CN region variant, a
non-conservative BoNT/C1 H.sub.CN region variant, a BoNT/C1
chimeric H.sub.CN region, an active BoNT/C1 H.sub.CN region
fragment, or any combination thereof. In still another aspect of
this embodiment, a BoNT/C1 H.sub.CN region comprises amino acids
869-1111 of a non-naturally occurring BoNT/C1 H.sub.CN region
variant of SEQ ID NO: 3, such as, e.g., amino acids 869-1111 of a
conservative BoNT/C1 H.sub.CN region variant of SEQ ID NO: 3, amino
acids 869-1111 of a non-conservative BoNT/C1 H.sub.CN region
variant of SEQ ID NO: 3, amino acids 869-1111 of an active BoNT/C1
H.sub.CN region fragment of SEQ ID NO: 3, or any combination
thereof.
[0146] In other aspects of this embodiment, a BoNT/C1 H.sub.CN
region comprises a polypeptide having, e.g., at least 70% amino
acid identity with amino acids 869-1111 of SEQ ID NO: 3, at least
75% amino acid identity with amino acids 869-1111 of SEQ ID NO: 3,
at least 80% amino acid identity with amino acids 869-1111 of SEQ
ID NO: 3, at least 85% amino acid identity with amino acids
869-1111 of SEQ ID NO: 3, at least 90% amino acid identity with
amino acids 869-1111 of SEQ ID NO: 3 or at least 95% amino acid
identity with amino acids 869-1111 of SEQ ID NO: 3. In yet other
aspects of this embodiment, a BoNT/C1 H.sub.CN region comprises a
polypeptide having, e.g., at most 70% amino acid identity with
amino acids 869-1111 of SEQ ID NO: 3, at most 75% amino acid
identity with amino acids 869-1111 of SEQ ID NO: 3, at most 80%
amino acid identity with amino acids 869-1111 of SEQ ID NO: 3, at
most 85% amino acid identity with amino acids 869-1111 of SEQ ID
NO: 3, at most 90% amino acid identity with amino acids 869-1111 of
SEQ ID NO: 3 or at most 95% amino acid identity with amino acids
869-1111 of SEQ ID NO: 3.
[0147] In other aspects of this embodiment, a BoNT/C1 H.sub.CN
region comprises a polypeptide having, e.g., at most one, two,
three, four, five, six, seven, eight, nine, 10, 20, 30, 40, 50,
100, or 200 non-contiguous amino acid substitutions relative to
amino acids 869-1111 of SEQ ID NO: 3. In other aspects of this
embodiment, a BoNT/C1 H.sub.CN region comprises a polypeptide
having, e.g., at least one, two, three, four, five, six, seven,
eight, nine, 10, 20, 30, 40, 50 or 100 non-contiguous amino acid
substitutions relative to amino acids 869-1111 of SEQ ID NO: 3. In
yet other aspects of this embodiment, a BoNT/C1 H.sub.CN region
comprises a polypeptide having, e.g., at most one, two, three,
four, five, six, seven, eight, nine, 10, 20, 30, 40, 50 or 100
non-contiguous amino acid deletions relative to amino acids
869-1111 of SEQ ID NO: 3. In other aspects of this embodiment, a
BoNT/C1 H.sub.CN region comprises a polypeptide having, e.g., at
least one, two, three, four, five, six, seven, eight, nine, 10, 20,
30, 40, 50 or 100 non-contiguous amino acid deletions relative to
amino acids 869-1111 of SEQ ID NO: 3. In still other aspects of
this embodiment, a BoNT/C1 H.sub.CN region comprises a polypeptide
having, e.g., at most one, two, three, four, five, six, seven,
eight, nine, 10, 20, 30, 40, 50 or 100 non-contiguous amino acid
additions relative to amino acids 869-1111 of SEQ ID NO: 3. In
other aspects of this embodiment, a BoNT/C1 H.sub.CN region
comprises a polypeptide having, e.g., at least one, two, three,
four, five, six, seven, eight, nine, 10, 20, 30, 40, 50 or 100
non-contiguous amino acid additions relative to amino acids
869-1111 of SEQ ID NO: 3.
[0148] In other aspects of this embodiment, a BoNT/C1 H.sub.CN
region comprises a polypeptide having, e.g., at most one, two,
three, four, five, six, seven, eight, nine, 10, 20, 30, 40, 50 or
100 contiguous amino acid substitutions relative to amino acids
869-1111 of SEQ ID NO: 3. In other aspects of this embodiment, a
BoNT/C1 H.sub.CN region comprises a polypeptide having, e.g., at
least one, two, three, four, five, six, seven, eight, nine, 10, 20,
30, 40, 50 or 100 contiguous amino acid substitutions relative to
amino acids 869-1111 of SEQ ID NO: 3. In yet other aspects of this
embodiment, a BoNT/C1 H.sub.CN region comprises a polypeptide
having, e.g., at most one, two, three, four, five, six, seven,
eight, nine, 10, 20, 30, 40, 50 or 100 contiguous amino acid
deletions relative to amino acids 869-1111 of SEQ ID NO: 3. In
other aspects of this embodiment, a BoNT/C1 H.sub.CN region
comprises a polypeptide having, e.g., at least one, two, three,
four, five, six, seven, eight, nine, 10, 20, 30, 40, 50 or 100
contiguous amino acid deletions relative to amino acids 869-1111 of
SEQ ID NO: 3. In still other aspects of this embodiment, a BoNT/C1
H.sub.CN region comprises a polypeptide having, e.g., at most one,
two, three, four, five, six, seven, eight, nine, 10, 20, 30, 40, 50
or 100 contiguous amino acid additions relative to amino acids
869-1111 of SEQ ID NO: 3. In other aspects of this embodiment, a
BoNT/C1 H.sub.CN region comprises a polypeptide having, e.g., at
least one, two, three, four, five, six, seven, eight, nine, 10, 20,
30, 40, 50 or 100 contiguous amino acid additions relative to amino
acids 869-1111 of SEQ ID NO: 3.
[0149] In another embodiment, a Clostridial toxin translocation
domain comprises a BoNT/D H.sub.CN region. In an aspect of this
embodiment, a BoNT/D H.sub.CN region comprises amino acids 865-1098
of SEQ ID NO: 4. In another aspect of this embodiment, a BoNT/D
H.sub.CN region comprises a naturally occurring BoNT/D H.sub.CN
region variant, such as, e.g., a H.sub.CN region from a BoNT/D
isoform or a H.sub.CN region from a BoNT/D subtype. In another
aspect of this embodiment, a BoNT/D H.sub.CN region comprises amino
acids 865-1098 of a naturally occurring BoNT/D H.sub.CN region
variant of SEQ ID NO: 4, such as, e.g., amino acids 865-1098 of a
BoNT/D isoform of SEQ ID NO: 4 or amino acids 865-1098 of a BoNT/D
subtype of SEQ ID NO: 4. In still another aspect of this
embodiment, a BoNT/D H.sub.CN region comprises a non-naturally
occurring BoNT/D H.sub.CN region variant, such as, e.g., a
conservative BoNT/D H.sub.CN region variant, a non-conservative
BoNT/D H.sub.CN region variant, a BoNT/D chimeric H.sub.CN region,
an active BoNT/D H.sub.CN region fragment, or any combination
thereof. In still another aspect of this embodiment, a BoNT/D
H.sub.CN region comprises amino acids 865-1098 of a non-naturally
occurring BoNT/D H.sub.CN region variant of SEQ ID NO: 4, such as,
e.g., amino acids 865-1098 of a conservative BoNT/D H.sub.CN region
variant of SEQ ID NO: 4, amino acids 865-1098 of a non-conservative
BoNT/D H.sub.CN region variant of SEQ ID NO: 4, amino acids
865-1098 of an active BoNT/D H.sub.CN region fragment of SEQ ID NO:
4, or any combination thereof.
[0150] In other aspects of this embodiment, a BoNT/D H.sub.CN
region comprises a polypeptide having, e.g., at least 70% amino
acid identity with amino acids 865-1098 of SEQ ID NO: 4, at least
75% amino acid identity with amino acids 865-1098 of SEQ ID NO: 4,
at least 80% amino acid identity with amino acids 865-1098 of SEQ
ID NO: 4, at least 85% amino acid identity with amino acids
865-1098 of SEQ ID NO: 4, at least 90% amino acid identity with
amino acids 865-1098 of SEQ ID NO: 4 or at least 95% amino acid
identity with amino acids 865-1098 of SEQ ID NO: 4. In yet other
aspects of this embodiment, a BoNT/D H.sub.CN region comprises a
polypeptide having, e.g., at most 70% amino acid identity with
amino acids 865-1098 of SEQ ID NO: 4, at most 75% amino acid
identity with amino acids 865-1098 of SEQ ID NO: 4, at most 80%
amino acid identity with amino acids 865-1098 of SEQ ID NO: 4, at
most 85% amino acid identity with amino acids 865-1098 of SEQ ID
NO: 4, at most 90% amino acid identity with amino acids 865-1098 of
SEQ ID NO: 4 or at most 95% amino acid identity with amino acids
865-1098 of SEQ ID NO: 4.
[0151] In other aspects of this embodiment, a BoNT/D H.sub.CN
region comprises a polypeptide having, e.g., at most one, two,
three, four, five, six, seven, eight, nine, 10, 20, 30, 40, 50,
100, or 200 non-contiguous amino acid substitutions relative to
amino acids 865-1098 of SEQ ID NO: 4. In other aspects of this
embodiment, a BoNT/D H.sub.CN region comprises a polypeptide
having, e.g., at least one, two, three, four, five, six, seven,
eight, nine, 10, 20, 30, 40, 50 or 100 non-contiguous amino acid
substitutions relative to amino acids 865-1098 of SEQ ID NO: 4. In
yet other aspects of this embodiment, a BoNT/D H.sub.CN region
comprises a polypeptide having, e.g., at most one, two, three,
four, five, six, seven, eight, nine, 10, 20, 30, 40, 50 or 100
non-contiguous amino acid deletions relative to amino acids
865-1098 of SEQ ID NO: 4. In other aspects of this embodiment, a
BoNT/D H.sub.CN region comprises a polypeptide having, e.g., at
least one, two, three, four, five, six, seven, eight, nine, 10, 20,
30, 40, 50 or 100 non-contiguous amino acid deletions relative to
amino acids 865-1098 of SEQ ID NO: 4. In still other aspects of
this embodiment, a BoNT/D H.sub.CN region comprises a polypeptide
having, e.g., at most one, two, three, four, five, six, seven,
eight, nine, 10, 20, 30, 40, 50 or 100 non-contiguous amino acid
additions relative to amino acids 865-1098 of SEQ ID NO: 4. In
other aspects of this embodiment, a BoNT/D H.sub.CN region
comprises a polypeptide having, e.g., at least one, two, three,
four, five, six, seven, eight, nine, 10, 20, 30, 40, 50 or 100
non-contiguous amino acid additions relative to amino acids
865-1098 of SEQ ID NO: 4.
[0152] In other aspects of this embodiment, a BoNT/D H.sub.CN
region comprises a polypeptide having, e.g., at most one, two,
three, four, five, six, seven, eight, nine, 10, 20, 30, 40, 50 or
100 contiguous amino acid substitutions relative to amino acids
865-1098 of SEQ ID NO: 4. In other aspects of this embodiment, a
BoNT/D H.sub.CN region comprises a polypeptide having, e.g., at
least one, two, three, four, five, six, seven, eight, nine, 10, 20,
30, 40, 50 or 100 contiguous amino acid substitutions relative to
amino acids 865-1098 of SEQ ID NO: 4. In yet other aspects of this
embodiment, a BoNT/D H.sub.CN region comprises a polypeptide
having, e.g., at most one, two, three, four, five, six, seven,
eight, nine, 10, 20, 30, 40, 50 or 100 contiguous amino acid
deletions relative to amino acids 865-1098 of SEQ ID NO: 4. In
other aspects of this embodiment, a BoNT/D H.sub.CN region
comprises a polypeptide having, e.g., at least one, two, three,
four, five, six, seven, eight, nine, 10, 20, 30, 40, 50 or 100
contiguous amino acid deletions relative to amino acids 865-1098 of
SEQ ID NO: 4. In still other aspects of this embodiment, a BoNT/D
H.sub.CN region comprises a polypeptide having, e.g., at most one,
two, three, four, five, six, seven, eight, nine, 10, 20, 30, 40, 50
or 100 contiguous amino acid additions relative to amino acids
865-1098 of SEQ ID NO: 4. In other aspects of this embodiment, a
BoNT/D H.sub.CN region comprises a polypeptide having, e.g., at
least one, two, three, four, five, six, seven, eight, nine, 10, 20,
30, 40, 50 or 100 contiguous amino acid additions relative to amino
acids 865-1098 of SEQ ID NO: 4.
[0153] In another embodiment, a Clostridial toxin translocation
domain comprises a BoNT/E H.sub.CN region. In an aspect of this
embodiment, a BoNT/E H.sub.CN region comprises amino acids 848-1085
of SEQ ID NO: 5. In another aspect of this embodiment, a BoNT/E
H.sub.CN region comprises a naturally occurring BoNT/E H.sub.CN
region variant, such as, e.g., a H.sub.CN region from a BoNT/E
isoform or a H.sub.CN region from a BoNT/E subtype. In another
aspect of this embodiment, a BoNT/E H.sub.CN region comprises amino
acids 848-1085 of a naturally occurring BoNT/E H.sub.CN region
variant of SEQ ID NO: 5, such as, e.g., amino acids 848-1085 of a
BoNT/E isoform of SEQ ID NO: 5 or amino acids 848-1085 of a BoNT/E
subtype of SEQ ID NO: 5. In still another aspect of this
embodiment, a BoNT/E H.sub.CN region comprises a non-naturally
occurring BoNT/E H.sub.CN region variant, such as, e.g., a
conservative BoNT/E H.sub.CN region variant, a non-conservative
BoNT/E H.sub.CN region variant, a BoNT/E chimeric H.sub.CN region,
an active BoNT/E H.sub.CN region fragment, or any combination
thereof. In still another aspect of this embodiment, a BoNT/E
H.sub.CN region comprises amino acids 848-1085 of a non-naturally
occurring BoNT/E H.sub.CN region variant of SEQ ID NO: 5, such as,
e.g., amino acids 848-1085 of a conservative BoNT/E H.sub.CN region
variant of SEQ ID NO: 5, amino acids 848-1085 of a non-conservative
BoNT/E H.sub.CN region variant of SEQ ID NO: 5, amino acids
848-1085 of an active BoNT/E H.sub.CN region fragment of SEQ ID NO:
5, or any combination thereof.
[0154] In other aspects of this embodiment, a BoNT/E H.sub.CN
region comprises a polypeptide having, e.g., at least 70% amino
acid identity with amino acids 848-1085 of SEQ ID NO: 5, at least
75% amino acid identity with amino acids 848-1085 of SEQ ID NO: 5,
at least 80% amino acid identity with amino acids 848-1085 of SEQ
ID NO: 5, at least 85% amino acid identity with amino acids
848-1085 of SEQ ID NO: 5, at least 90% amino acid identity with
amino acids 848-1085 of SEQ ID NO: 5 or at least 95% amino acid
identity with amino acids 848-1085 of SEQ ID NO: 5. In yet other
aspects of this embodiment, a BoNT/E H.sub.CN region comprises a
polypeptide having, e.g., at most 70% amino acid identity with
amino acids 848-1085 of SEQ ID NO: 5, at most 75% amino acid
identity with amino acids 848-1085 of SEQ ID NO: 5, at most 80%
amino acid identity with amino acids 848-1085 of SEQ ID NO: 5, at
most 85% amino acid identity with amino acids 848-1085 of SEQ ID
NO: 5, at most 90% amino acid identity with amino acids 848-1085 of
SEQ ID NO: 5 or at most 95% amino acid identity with amino acids
848-1085 of SEQ ID NO: 5.
[0155] In other aspects of this embodiment, a BoNT/E H.sub.CN
region comprises a polypeptide having, e.g., at most one, two,
three, four, five, six, seven, eight, nine, 10, 20, 30, 40, 50,
100, or 200 non-contiguous amino acid substitutions relative to
amino acids 848-1085 of SEQ ID NO: 5. In other aspects of this
embodiment, a BoNT/E H.sub.CN region comprises a polypeptide
having, e.g., at least one, two, three, four, five, six, seven,
eight, nine, 10, 20, 30, 40, 50 or 100 non-contiguous amino acid
substitutions relative to amino acids 848-1085 of SEQ ID NO: 5. In
yet other aspects of this embodiment, a BoNT/E H.sub.CN region
comprises a polypeptide having, e.g., at most one, two, three,
four, five, six, seven, eight, nine, 10, 20, 30, 40, 50 or 100
non-contiguous amino acid deletions relative to amino acids
848-1085 of SEQ ID NO: 5. In other aspects of this embodiment, a
BoNT/E H.sub.CN region comprises a polypeptide having, e.g., at
least one, two, three, four, five, six, seven, eight, nine, 10, 20,
30, 40, 50 or 100 non-contiguous amino acid deletions relative to
amino acids 848-1085 of SEQ ID NO: 5. In still other aspects of
this embodiment, a BoNT/E H.sub.CN region comprises a polypeptide
having, e.g., at most one, two, three, four, five, six, seven,
eight, nine, 10, 20, 30, 40, 50 or 100 non-contiguous amino acid
additions relative to amino acids 848-1085 of SEQ ID NO: 5. In
other aspects of this embodiment, a BoNT/E H.sub.CN region
comprises a polypeptide having, e.g., at least one, two, three,
four, five, six, seven, eight, nine, 10, 20, 30, 40, 50 or 100
non-contiguous amino acid additions relative to amino acids
848-1085 of SEQ ID NO: 5.
[0156] In other aspects of this embodiment, a BoNT/E H.sub.CN
region comprises a polypeptide having, e.g., at most one, two,
three, four, five, six, seven, eight, nine, 10, 20, 30, 40, 50 or
100 contiguous amino acid substitutions relative to amino acids
848-1085 of SEQ ID NO: 5. In other aspects of this embodiment, a
BoNT/E H.sub.CN region comprises a polypeptide having, e.g., at
least one, two, three, four, five, six, seven, eight, nine, 10, 20,
30, 40, 50 or 100 contiguous amino acid substitutions relative to
amino acids 848-1085 of SEQ ID NO: 5. In yet other aspects of this
embodiment, a BoNT/E H.sub.CN region comprises a polypeptide
having, e.g., at most one, two, three, four, five, six, seven,
eight, nine, 10, 20, 30, 40, 50 or 100 contiguous amino acid
deletions relative to amino acids 848-1085 of SEQ ID NO: 5. In
other aspects of this embodiment, a BoNT/E H.sub.CN region
comprises a polypeptide having, e.g., at least one, two, three,
four, five, six, seven, eight, nine, 10, 20, 30, 40, 50 or 100
contiguous amino acid deletions relative to amino acids 848-1085 of
SEQ ID NO: 5. In still other aspects of this embodiment, a BoNT/E
H.sub.CN region comprises a polypeptide having, e.g., at most one,
two, three, four, five, six, seven, eight, nine, 10, 20, 30, 40, 50
or 100 contiguous amino acid additions relative to amino acids
848-1085 of SEQ ID NO: 5. In other aspects of this embodiment, a
BoNT/E H.sub.CN region comprises a polypeptide having, e.g., at
least one, two, three, four, five, six, seven, eight, nine, 10, 20,
30, 40, 50 or 100 contiguous amino acid additions relative to amino
acids 848-1085 of SEQ ID NO: 5.
[0157] In another embodiment, a Clostridial toxin translocation
domain comprises a BoNT/F H.sub.CN region. In an aspect of this
embodiment, a BoNT/F H.sub.CN region comprises amino acids 867-1105
of SEQ ID NO: 6. In another aspect of this embodiment, a BoNT/F
H.sub.CN region comprises a naturally occurring BoNT/F H.sub.CN
region variant, such as, e.g., a H.sub.CN region from a BoNT/F
isoform or a H.sub.CN region from a BoNT/F subtype. In another
aspect of this embodiment, a BoNT/F H.sub.CN region comprises amino
acids 867-1105 of a naturally occurring BoNT/F H.sub.CN region
variant of SEQ ID NO: 6, such as, e.g., amino acids 867-1105 of a
BoNT/F isoform of SEQ ID NO: 6 or amino acids 867-1105 of a BoNT/F
subtype of SEQ ID NO: 6. In still another aspect of this
embodiment, a BoNT/F H.sub.CN region comprises a non-naturally
occurring BoNT/F H.sub.CN region variant, such as, e.g., a
conservative BoNT/F H.sub.CN region variant, a non-conservative
BoNT/F H.sub.CN region variant, a BoNT/F chimeric H.sub.CN region,
an active BoNT/F H.sub.CN region fragment, or any combination
thereof. In still another aspect of this embodiment, a BoNT/F
H.sub.CN region comprises amino acids 867-1105 of a non-naturally
occurring BoNT/F H.sub.CN region variant of SEQ ID NO: 6, such as,
e.g., amino acids 867-1105 of a conservative BoNT/F H.sub.CN region
variant of SEQ ID NO: 6, amino acids 867-1105 of a non-conservative
BoNT/F H.sub.CN region variant of SEQ ID NO: 6, amino acids
867-1105 of an active BoNT/F H.sub.CN region fragment of SEQ ID NO:
6, or any combination thereof.
[0158] In other aspects of this embodiment, a BoNT/F H.sub.CN
region comprises a polypeptide having, e.g., at least 70% amino
acid identity with amino acids 867-1105 of SEQ ID NO: 6, at least
75% amino acid identity with amino acids 867-1105 of SEQ ID NO: 6,
at least 80% amino acid identity with amino acids 867-1105 of SEQ
ID NO: 6, at least 85% amino acid identity with amino acids
867-1105 of SEQ ID NO: 6, at least 90% amino acid identity with
amino acids 867-1105 of SEQ ID NO: 6 or at least 95% amino acid
identity with amino acids 867-1105 of SEQ ID NO: 6. In yet other
aspects of this embodiment, a BoNT/F H.sub.CN region comprises a
polypeptide having, e.g., at most 70% amino acid identity with
amino acids 867-1105 of SEQ ID NO: 6, at most 75% amino acid
identity with amino acids 867-1105 of SEQ ID NO: 6, at most 80%
amino acid identity with amino acids 867-1105 of SEQ ID NO: 6, at
most 85% amino acid identity with amino acids 867-1105 of SEQ ID
NO: 6, at most 90% amino acid identity with amino acids 867-1105 of
SEQ ID NO: 6 or at most 95% amino acid identity with amino acids
867-1105 of SEQ ID NO: 6.
[0159] In other aspects of this embodiment, a BoNT/F H.sub.CN
region comprises a polypeptide having, e.g., at most one, two,
three, four, five, six, seven, eight, nine, 10, 20, 30, 40, 50,
100, or 200 non-contiguous amino acid substitutions relative to
amino acids 867-1105 of SEQ ID NO: 6. In other aspects of this
embodiment, a BoNT/F H.sub.CN region comprises a polypeptide
having, e.g., at least one, two, three, four, five, six, seven,
eight, nine, 10, 20, 30, 40, 50 or 100 non-contiguous amino acid
substitutions relative to amino acids 867-1105 of SEQ ID NO: 6. In
yet other aspects of this embodiment, a BoNT/F H.sub.CN region
comprises a polypeptide having, e.g., at most one, two, three,
four, five, six, seven, eight, nine, 10, 20, 30, 40, 50 or 100
non-contiguous amino acid deletions relative to amino acids
867-1105 of SEQ ID NO: 6. In other aspects of this embodiment, a
BoNT/F H.sub.CN region comprises a polypeptide having, e.g., at
least one, two, three, four, five, six, seven, eight, nine, 10, 20,
30, 40, 50 or 100 non-contiguous amino acid deletions relative to
amino acids 867-1105 of SEQ ID NO: 6. In still other aspects of
this embodiment, a BoNT/F H.sub.CN region comprises a polypeptide
having, e.g., at most one, two, three, four, five, six, seven,
eight, nine, 10, 20, 30, 40, 50 or 100 non-contiguous amino acid
additions relative to amino acids 867-1105 of SEQ ID NO: 6. In
other aspects of this embodiment, a BoNT/F H.sub.CN region
comprises a polypeptide having, e.g., at least one, two, three,
four, five, six, seven, eight, nine, 10, 20, 30, 40, 50 or 100
non-contiguous amino acid additions relative to amino acids
867-1105 of SEQ ID NO: 6.
[0160] In other aspects of this embodiment, a BoNT/F H.sub.CN
region comprises a polypeptide having, e.g., at most one, two,
three, four, five, six, seven, eight, nine, 10, 20, 30, 40, 50 or
100 contiguous amino acid substitutions relative to amino acids
867-1105 of SEQ ID NO: 6. In other aspects of this embodiment, a
BoNT/F H.sub.CN region comprises a polypeptide having, e.g., at
least one, two, three, four, five, six, seven, eight, nine, 10, 20,
30, 40, 50 or 100 contiguous amino acid substitutions relative to
amino acids 867-1105 of SEQ ID NO: 6. In yet other aspects of this
embodiment, a BoNT/F H.sub.CN region comprises a polypeptide
having, e.g., at most one, two, three, four, five, six, seven,
eight, nine, 10, 20, 30, 40, 50 or 100 contiguous amino acid
deletions relative to amino acids 867-1105 of SEQ ID NO: 6. In
other aspects of this embodiment, a BoNT/F H.sub.CN region
comprises a polypeptide having, e.g., at least one, two, three,
four, five, six, seven, eight, nine, 10, 20, 30, 40, 50 or 100
contiguous amino acid deletions relative to amino acids 867-1105 of
SEQ ID NO: 6. In still other aspects of this embodiment, a BoNT/F
H.sub.CN region comprises a polypeptide having, e.g., at most one,
two, three, four, five, six, seven, eight, nine, 10, 20, 30, 40, 50
or 100 contiguous amino acid additions relative to amino acids
867-1105 of SEQ ID NO: 6. In other aspects of this embodiment, a
BoNT/F H.sub.CN region comprises a polypeptide having, e.g., at
least one, two, three, four, five, six, seven, eight, nine, 10, 20,
30, 40, 50 or 100 contiguous amino acid additions relative to amino
acids 867-1105 of SEQ ID NO: 6.
[0161] In another embodiment, a Clostridial toxin translocation
domain comprises a BoNT/G H.sub.CN region. In an aspect of this
embodiment, a BoNT/G H.sub.CN region comprises amino acids 866-1105
of SEQ ID NO: 7. In another aspect of this embodiment, a BoNT/G
H.sub.CN region comprises a naturally occurring BoNT/G H.sub.CN
region variant, such as, e.g., a H.sub.CN region from a BoNT/G
isoform or a H.sub.CN region from a BoNT/G subtype. In another
aspect of this embodiment, a BoNT/G H.sub.CN region comprises amino
acids 866-1105 of a naturally occurring BoNT/G H.sub.CN region
variant of SEQ ID NO: 7, such as, e.g., amino acids 866-1105 of a
BoNT/G isoform of SEQ ID NO: 7 or amino acids 866-1105 of a BoNT/G
subtype of SEQ ID NO: 7. In still another aspect of this
embodiment, a BoNT/G H.sub.CN region comprises a non-naturally
occurring BoNT/G H.sub.CN region variant, such as, e.g., a
conservative BoNT/G H.sub.CN region variant, a non-conservative
BoNT/G H.sub.CN region variant, a BoNT/G chimeric H.sub.CN region,
an active BoNT/G H.sub.CN region fragment, or any combination
thereof. In still another aspect of this embodiment, a BoNT/G
H.sub.CN region comprises amino acids 866-1105 of a non-naturally
occurring BoNT/G H.sub.CN region variant of SEQ ID NO: 7, such as,
e.g., amino acids 866-1105 of a conservative BoNT/G H.sub.CN region
variant of SEQ ID NO: 7, amino acids 866-1105 of a non-conservative
BoNT/G H.sub.CN region variant of SEQ ID NO: 7, amino acids
866-1105 of an active BoNT/G H.sub.CN region fragment of SEQ ID NO:
7, or any combination thereof.
[0162] In other aspects of this embodiment, a BoNT/G H.sub.CN
region comprises a polypeptide having, e.g., at least 70% amino
acid identity with amino acids 866-1105 of SEQ ID NO: 7, at least
75% amino acid identity with amino acids 866-1105 of SEQ ID NO: 7,
at least 80% amino acid identity with amino acids 866-1105 of SEQ
ID NO: 7, at least 85% amino acid identity with amino acids
866-1105 of SEQ ID NO: 7, at least 90% amino acid identity with
amino acids 866-1105 of SEQ ID NO: 7 or at least 95% amino acid
identity with amino acids 866-1105 of SEQ ID NO: 7. In yet other
aspects of this embodiment, a BoNT/G H.sub.CN region comprises a
polypeptide having, e.g., at most 70% amino acid identity with
amino acids 866-1105 of SEQ ID NO: 7, at most 75% amino acid
identity with amino acids 866-1105 of SEQ ID NO: 7, at most 80%
amino acid identity with amino acids 866-1105 of SEQ ID NO: 7, at
most 85% amino acid identity with amino acids 866-1105 of SEQ ID
NO: 7, at most 90% amino acid identity with amino acids 866-1105 of
SEQ ID NO: 7 or at most 95% amino acid identity with amino acids
866-1105 of SEQ ID NO: 7.
[0163] In other aspects of this embodiment, a BoNT/G H.sub.CN
region comprises a polypeptide having, e.g., at most one, two,
three, four, five, six, seven, eight, nine, 10, 20, 30, 40, 50,
100, or 200 non-contiguous amino acid substitutions relative to
amino acids 866-1105 of SEQ ID NO: 7. In other aspects of this
embodiment, a BoNT/G H.sub.CN region comprises a polypeptide
having, e.g., at least one, two, three, four, five, six, seven,
eight, nine, 10, 20, 30, 40, 50 or 100 non-contiguous amino acid
substitutions relative to amino acids 866-1105 of SEQ ID NO: 7. In
yet other aspects of this embodiment, a BoNT/G H.sub.CN region
comprises a polypeptide having, e.g., at most one, two, three,
four, five, six, seven, eight, nine, 10, 20, 30, 40, 50 or 100
non-contiguous amino acid deletions relative to amino acids
866-1105 of SEQ ID NO: 7. In other aspects of this embodiment, a
BoNT/G H.sub.CN region comprises a polypeptide having, e.g., at
least one, two, three, four, five, six, seven, eight, nine, 10, 20,
30, 40, 50 or 100 non-contiguous amino acid deletions relative to
amino acids 866-1105 of SEQ ID NO: 7. In still other aspects of
this embodiment, a BoNT/G H.sub.CN region comprises a polypeptide
having, e.g., at most one, two, three, four, five, six, seven,
eight, nine, 10, 20, 30, 40, 50 or 100 non-contiguous amino acid
additions relative to amino acids 866-1105 of SEQ ID NO: 7. In
other aspects of this embodiment, a BoNT/G H.sub.CN region
comprises a polypeptide having, e.g., at least one, two, three,
four, five, six, seven, eight, nine, 10, 20, 30, 40, 50 or 100
non-contiguous amino acid additions relative to amino acids
866-1105 of SEQ ID NO: 7.
[0164] In other aspects of this embodiment, a BoNT/G H.sub.CN
region comprises a polypeptide having, e.g., at most one, two,
three, four, five, six, seven, eight, nine, 10, 20, 30, 40, 50 or
100 contiguous amino acid substitutions relative to amino acids
866-1105 of SEQ ID NO: 7. In other aspects of this embodiment, a
BoNT/G H.sub.CN region comprises a polypeptide having, e.g., at
least one, two, three, four, five, six, seven, eight, nine, 10, 20,
30, 40, 50 or 100 contiguous amino acid substitutions relative to
amino acids 866-1105 of SEQ ID NO: 7. In yet other aspects of this
embodiment, a BoNT/G H.sub.CN region comprises a polypeptide
having, e.g., at most one, two, three, four, five, six, seven,
eight, nine, 10, 20, 30, 40, 50 or 100 contiguous amino acid
deletions relative to amino acids 866-1105 of SEQ ID NO: 7. In
other aspects of this embodiment, a BoNT/G H.sub.CN region
comprises a polypeptide having, e.g., at least one, two, three,
four, five, six, seven, eight, nine, 10, 20, 30, 40, 50 or 100
contiguous amino acid deletions relative to amino acids 866-1105 of
SEQ ID NO: 7. In still other aspects of this embodiment, a BoNT/G
H.sub.CN region comprises a polypeptide having, e.g., at most one,
two, three, four, five, six, seven, eight, nine, 10, 20, 30, 40, 50
or 100 contiguous amino acid additions relative to amino acids
866-1105 of SEQ ID NO: 7. In other aspects of this embodiment, a
BoNT/G H.sub.CN region comprises a polypeptide having, e.g., at
least one, two, three, four, five, six, seven, eight, nine, 10, 20,
30, 40, 50 or 100 contiguous amino acid additions relative to amino
acids 866-1105 of SEQ ID NO: 7.
[0165] In another embodiment, a Clostridial toxin translocation
domain comprises a TeNT H.sub.CN region. In an aspect of this
embodiment, a TeNT H.sub.CN region comprises amino acids 882-1127
of SEQ ID NO: 8. In another aspect of this embodiment, a TeNT
H.sub.CN region comprises a naturally occurring TeNT H.sub.CN
region variant, such as, e.g., a H.sub.CN region from a TeNT
isoform or a H.sub.CN region from a TeNT subtype. In another aspect
of this embodiment, a TeNT H.sub.CN region comprises amino acids
882-1127 of a naturally occurring TeNT H.sub.CN region variant of
SEQ ID NO: 8, such as, e.g., amino acids 882-1127 of a TeNT isoform
of SEQ ID NO: 8 or amino acids 882-1127 of a TeNT subtype of SEQ ID
NO: 8. In still another aspect of this embodiment, a TeNT H.sub.CN
region comprises a non-naturally occurring TeNT H.sub.CN region
variant, such as, e.g., a conservative TeNT H.sub.CN region
variant, a non-conservative TeNT H.sub.CN region variant, a TeNT
chimeric H.sub.CN region, an active TeNT H.sub.CN region fragment,
or any combination thereof. In still another aspect of this
embodiment, a TeNT H.sub.CN region comprises amino acids 882-1127
of a non-naturally occurring TeNT H.sub.CN region variant of SEQ ID
NO: 8, such as, e.g., amino acids 882-1127 of a conservative TeNT
H.sub.CN region variant of SEQ ID NO: 8, amino acids 882-1127 of a
non-conservative TeNT H.sub.CN region variant of SEQ ID NO: 8,
amino acids 882-1127 of an active TeNT H.sub.CN region fragment of
SEQ ID NO: 8, or any combination thereof.
[0166] In other aspects of this embodiment, a TeNT H.sub.CN region
comprises a polypeptide having, e.g., at least 70% amino acid
identity with amino acids 882-1127 of SEQ ID NO: 8, at least 75%
amino acid identity with amino acids 882-1127 of SEQ ID NO: 8, at
least 80% amino acid identity with amino acids 882-1127 of SEQ ID
NO: 8, at least 85% amino acid identity with amino acids 882-1127
of SEQ ID NO: 8, at least 90% amino acid identity with amino acids
882-1127 of SEQ ID NO: 8 or at least 95% amino acid identity with
amino acids 882-1127 of SEQ ID NO: 8. In yet other aspects of this
embodiment, a TeNT H.sub.CN region comprises a polypeptide having,
e.g., at most 70% amino acid identity with amino acids 882-1127 of
SEQ ID NO: 8, at most 75% amino acid identity with amino acids
882-1127 of SEQ ID NO: 8, at most 80% amino acid identity with
amino acids 882-1127 of SEQ ID NO: 8, at most 85% amino acid
identity with amino acids 882-1127 of SEQ ID NO: 8, at most 90%
amino acid identity with amino acids 882-1127 of SEQ ID NO: 8 or at
most 95% amino acid identity with amino acids 882-1127 of SEQ ID
NO: 8.
[0167] In other aspects of this embodiment, a TeNT H.sub.CN region
comprises a polypeptide having, e.g., at most one, two, three,
four, five, six, seven, eight, nine, 10, 20, 30, 40, 50, 100, or
200 non-contiguous amino acid substitutions relative to amino acids
882-1127 of SEQ ID NO: 8. In other aspects of this embodiment, a
TeNT H.sub.CN region comprises a polypeptide having, e.g., at least
one, two, three, four, five, six, seven, eight, nine, 10, 20, 30,
40, 50 or 100 non-contiguous amino acid substitutions relative to
amino acids 882-1127 of SEQ ID NO: 8. In yet other aspects of this
embodiment, a TeNT H.sub.CN region comprises a polypeptide having,
e.g., at most one, two, three, four, five, six, seven, eight, nine,
10, 20, 30, 40, 50 or 100 non-contiguous amino acid deletions
relative to amino acids 882-1127 of SEQ ID NO: 8. In other aspects
of this embodiment, a TeNT H.sub.CN region comprises a polypeptide
having, e.g., at least one, two, three, four, five, six, seven,
eight, nine, 10, 20, 30, 40, 50 or 100 non-contiguous amino acid
deletions relative to amino acids 882-1127 of SEQ ID NO: 8. In
still other aspects of this embodiment, a TeNT H.sub.CN region
comprises a polypeptide having, e.g., at most one, two, three,
four, five, six, seven, eight, nine, 10, 20, 30, 40, 50 or 100
non-contiguous amino acid additions relative to amino acids
882-1127 of SEQ ID NO: 8. In other aspects of this embodiment, a
TeNT H.sub.CN region comprises a polypeptide having, e.g., at least
one, two, three, four, five, six, seven, eight, nine, 10, 20, 30,
40, 50 or 100 non-contiguous amino acid additions relative to amino
acids 882-1127 of SEQ ID NO: 8.
[0168] In other aspects of this embodiment, a TeNT H.sub.CN region
comprises a polypeptide having, e.g., at most one, two, three,
four, five, six, seven, eight, nine, 10, 20, 30, 40, 50 or 100
contiguous amino acid substitutions relative to amino acids
882-1127 of SEQ ID NO: 8. In other aspects of this embodiment, a
TeNT H.sub.CN region comprises a polypeptide having, e.g., at least
one, two, three, four, five, six, seven, eight, nine, 10, 20, 30,
40, 50 or 100 contiguous amino acid substitutions relative to amino
acids 882-1127 of SEQ ID NO: 8. In yet other aspects of this
embodiment, a TeNT H.sub.CN region comprises a polypeptide having,
e.g., at most one, two, three, four, five, six, seven, eight, nine,
10, 20, 30, 40, 50 or 100 contiguous amino acid deletions relative
to amino acids 882-1127 of SEQ ID NO: 8. In other aspects of this
embodiment, a TeNT H.sub.CN region comprises a polypeptide having,
e.g., at least one, two, three, four, five, six, seven, eight,
nine, 10, 20, 30, 40, 50 or 100 contiguous amino acid deletions
relative to amino acids 882-1127 of SEQ ID NO: 8. In still other
aspects of this embodiment, a TeNT H.sub.CN region comprises a
polypeptide having, e.g., at most one, two, three, four, five, six,
seven, eight, nine, 10, 20, 30, 40, 50 or 100 contiguous amino acid
additions relative to amino acids 882-1127 of SEQ ID NO: 8. In
other aspects of this embodiment, a TeNT H.sub.CN region comprises
a polypeptide having, e.g., at least one, two, three, four, five,
six, seven, eight, nine, 10, 20, 30, 40, 50 or 100 contiguous amino
acid additions relative to amino acids 882-1127 of SEQ ID NO:
8.
[0169] The fusion of the membrane of enveloped viruses to a
cellular membrane is an essential step in the release of the viral
capsule into the cytoplasm of the host cell. This fusion event is
mediated by a fusogenic peptide segment present in viral
glycoproteins located on the viral membrane and involves either a
pH-dependent or pH-independent process, see, e.g., Frederick M.
Hughson, Structural Characterization of Viral Fusion Proteins,
5.beta.) Curr. Biol. 158-274 (1128); Trudy G. Morrison, Structure
and Function of a Paramyxovirus Fusion Protein, 1304(1) Biochim.
Biophys. Acta. 73-84; David J. Schibli and Winfried Weissenhorn,
Class I and Class II Viral Fusion Protein Structures Reveal Similar
Principles in Membrane Fusion, 21(6) Mol. Membr. Biol. 361-371
(1137). The fusogenic peptide domain comprises a hydrophobic,
glycine-rich peptide of approximately 20-30 amino acids that assist
in the insertion of the viral capsule into a cellular membrane.
Thus, an enveloped virus fusogenic peptide domain can be useful as
a translocation facilitating domain.
[0170] An enveloped virus fusogenic peptide domain includes,
without limitation, naturally occurring enveloped virus fusogenic
peptide domain variants, such as, e.g., enveloped virus fusogenic
peptide domain isoforms and enveloped virus fusogenic peptide
domain subtypes; non-naturally occurring enveloped virus fusogenic
peptide domain variants, such as, e.g., conservative enveloped
virus fusogenic peptide domain variants, non-conservative enveloped
virus fusogenic peptide domain variants, enveloped virus fusogenic
peptide domain chimerics, active enveloped virus fusogenic peptide
domain fragments thereof, or any combination thereof.
[0171] As used herein, the term "enveloped virus fusogenic peptide
domain variant," whether naturally-occurring or
non-naturally-occurring, means an enveloped virus fusogenic peptide
domain that has at least one amino acid change from the
corresponding region of the disclosed reference sequences and can
be described in percent identity to the corresponding region of
that reference sequence. Unless expressly indicated, all
Clostridial toxin H.sub.CN region variants disclosed in the present
specification are capable of further facilitating the translocation
step of the intoxication process that mediates Clostridial toxin
light chain translocation. As non-limiting examples, an Influenza
virus A fusogenic peptide domain variant comprising SEQ ID NO: 87
will have at least one amino acid difference, such as, e.g., an
amino acid substitution, deletion or addition, as compared to SEQ
ID NO: 87; a Semliki Forest virus fusogenic peptide domain variant
comprising SEQ ID NO: 92 will have at least one amino acid
difference, such as, e.g., an amino acid substitution, deletion or
addition, as compared to SEQ ID NO: 92; an Eastern equine
encephalitis virus fusogenic peptide domain variant comprising SEQ
ID NO: 94 will have at least one amino acid difference, such as,
e.g., an amino acid substitution, deletion or addition, as compared
to SEQ ID NO: 94; a Venezuelan equine encephalitis virus fusogenic
peptide domain variant comprising SEQ ID NO: 102 will have at least
one amino acid difference, such as, e.g., an amino acid
substitution, deletion or addition, as compared to SEQ ID NO: 102;
a Vesicular stomatitis virus fusogenic peptide domain variant
comprising SEQ ID NO: 119 will have at least one amino acid
difference, such as, e.g., an amino acid substitution, deletion or
addition, as compared to SEQ ID NO: 119; a Sendai virus fusogenic
peptide domain variant comprising SEQ ID NO: 130 will have at least
one amino acid difference, such as, e.g., an amino acid
substitution, deletion or addition, as compared to SEQ ID NO: 130;
a Canine distemper virus fusogenic peptide domain variant
comprising SEQ ID NO: 137 will have at least one amino acid
difference, such as, e.g., an amino acid substitution, deletion or
addition, as compared to SEQ ID NO: 137; a Newcastle disease virus
fusogenic peptide domain variant comprising SEQ ID NO: 147 will
have at least one amino acid difference, such as, e.g., an amino
acid substitution, deletion or addition, as compared to SEQ ID NO:
147; and a Hendra virus fusogenic peptide domain variant comprising
SEQ ID NO: 160 will have at least one amino acid difference, such
as, e.g., an amino acid substitution, deletion or addition, as
compared to SEQ ID NO: 160.
[0172] It is recognized by those of skill in the art that for each
enveloped virus there can be naturally occurring fusogenic peptide
domain variants that differ somewhat in their amino acid sequence,
and also in the nucleic acids encoding these proteins. For example,
at least five naturally-occurring variants of the fusogenic peptide
domain present in the Influenza A virus haemagglutinin are known
(SEQ ID NO: 87 to SEQ ID NO: 91); at least six naturally-occurring
variants of the fusogenic peptide domain present in the Eastern
equine encephalitis virus E1 protein are known (SEQ ID NO: 94 to
SEQ ID NO: 99); at least eight naturally-occurring variants of the
fusogenic peptide domain present in the Venezuelan equine
encephalitis virus E1 protein are known (SEQ ID NO: 102 to SEQ ID
NO: 109); at least seven naturally-occurring variants of the
fusogenic peptide domain present in the Vesicular stomatitis virus
(VSV) glycoprotein G are known (SEQ ID NO: 119 to SEQ ID NO: 125);
and at least eleven naturally-occurring variants of the fusogenic
peptide domain present in the Newcastle disease virus F protein are
known (SEQ ID NO: 147 to SEQ ID NO: 157). As used herein, the term
"enveloped virus fusogenic peptide domain variant" means any
enveloped virus fusogenic peptide domain produced by a
naturally-occurring process, including, without limitation,
enveloped virus fusogenic peptide domain isoforms produced from
alternatively-spliced transcripts, enveloped virus fusogenic
peptide domain isoforms produced by spontaneous mutation and
enveloped virus fusogenic peptide domain subtypes. A naturally
occurring enveloped virus fusogenic peptide domain variant can
function in substantially the same manner as the reference
enveloped virus fusogenic peptide domain on which the naturally
occurring enveloped virus fusogenic peptide domain variant is
based, and can be substituted for the reference enveloped virus
fusogenic peptide domain in any aspect of the present invention. A
naturally occurring enveloped virus fusogenic peptide domain
variant may substitute one or more amino acids, two or more amino
acids, three or more amino acids, four or more amino acids, five or
more amino acids or ten or more amino acids from the reference
enveloped virus fusogenic peptide domain on which the naturally
occurring enveloped virus fusogenic peptide domain is based. A
naturally occurring enveloped virus fusogenic peptide domain
variant can also substitute at least 2 contiguous amino acids, at
least 3 contiguous amino acids, at least 4 contiguous amino acids
or at least 5 contiguous amino acids from the reference enveloped
virus fusogenic peptide domain on which the naturally occurring
enveloped virus fusogenic peptide domain variant is based, that
possess at least 50% amino acid identity, 65% amino acid identity,
75% amino acid identity, 85% amino acid identity or 95% amino acid
identity to the reference enveloped virus fusogenic peptide domain
on which the naturally occurring enveloped virus fusogenic peptide
domain variant is based.
[0173] A non-limiting examples of a naturally occurring enveloped
virus fusogenic peptide domain variant is a enveloped virus
fusogenic peptide domain isoform such as, e.g., an influenzavirus
fusogenic peptide domain isoform, an alphavirus fusogenic peptide
domain isoform, a vesiculovirus fusogenic peptide domain isoform, a
respirovirus fusogenic peptide domain isoform, a morbillivirus
fusogenic peptide domain isoform, an avulavirus fusogenic peptide
domain isoform, a henipavirus fusogenic peptide domain isoform, a
metapneumovirus fusogenic peptide domain isoform and a foamy virus
fusogenic peptide domain isoform. An enveloped virus fusogenic
peptide domain isoform can function in substantially the same
manner as the reference enveloped virus fusogenic peptide domain on
which the enveloped virus fusogenic peptide domain isoform is
based, and can be substituted for the reference enveloped virus
fusogenic peptide domain in any aspect of the present
invention.
[0174] A non-limiting examples of a naturally occurring enveloped
virus fusogenic peptide domain variant is a enveloped virus
fusogenic peptide domain subtype such as, e.g., an influenzavirus
fusogenic peptide domain subtype, an alphavirus fusogenic peptide
domain subtype, a vesiculovirus fusogenic peptide domain subtype, a
respirovirus fusogenic peptide domain subtype, a morbillivirus
fusogenic peptide domain subtype, an avulavirus fusogenic peptide
domain subtype, a henipavirus fusogenic peptide domain subtype, a
metapneumovirus fusogenic peptide domain subtype and a foamy virus
fusogenic peptide domain subtype. An enveloped virus fusogenic
peptide domain subtype can function in substantially the same
manner as the reference enveloped virus fusogenic peptide domain on
which the enveloped virus fusogenic peptide domain subtype is
based, and can be substituted for the reference enveloped virus
fusogenic peptide domain in any aspect of the present
invention.
[0175] As used herein, the term "non-naturally occurring enveloped
virus fusogenic peptide domain variant" means any enveloped virus
fusogenic peptide domain produced with the aid of human
manipulation, including, without limitation, enveloped virus
fusogenic peptide domains produced by genetic engineering using
random mutagenesis or rational design and enveloped virus fusogenic
peptide domains produced by chemical synthesis. Non-limiting
examples of non-naturally occurring enveloped virus fusogenic
peptide domain variants include, e.g., conservative enveloped virus
fusogenic peptide domain variants, non-conservative enveloped virus
fusogenic peptide domain variants, enveloped virus fusogenic
peptide domain chimeric variants and active enveloped virus
fusogenic peptide domain fragments.
[0176] As used herein, the term "conservative enveloped virus
fusogenic peptide domain variant" means a enveloped virus fusogenic
peptide domain that has at least one amino acid substituted by
another amino acid or an amino acid analog that has at least one
property similar to that of the original amino acid from the
reference enveloped virus fusogenic peptide domain sequence.
Examples of properties include, without limitation, similar size,
topography, charge, hydrophobicity, hydrophilicity, lipophilicity,
covalent-bonding capacity, hydrogen-bonding capacity, a
physicochemical property, of the like, or any combination thereof.
A conservative enveloped virus fusogenic peptide domain variant can
function in substantially the same manner as the reference
enveloped virus fusogenic peptide domain on which the conservative
enveloped virus fusogenic peptide domain variant is based, and can
be substituted for the reference enveloped virus fusogenic peptide
domain in any aspect of the present invention. A conservative
enveloped virus fusogenic peptide domain variant may substitute one
or more amino acids, two or more amino acids, three or more amino
acids, four or more amino acids, five or more amino acids or ten or
more amino acids from the reference enveloped virus fusogenic
peptide domain on which the conservative enveloped virus fusogenic
peptide domain variant is based. A conservative enveloped virus
fusogenic peptide domain variant can also substitute at least 2
contiguous amino acids, at least 3 contiguous amino acids, at least
4 contiguous amino acids or at least 5 contiguous amino acids from
the reference enveloped virus fusogenic peptide domain on which the
conservative enveloped virus fusogenic peptide domain variant is
based, that possess at least 50% amino acid identity, 65% amino
acid identity, 75% amino acid identity, 85% amino acid identity or
95% amino acid identity to the reference enveloped virus fusogenic
peptide domain on which the conservative enveloped virus fusogenic
peptide domain variant is based. Non-limiting examples of a
conservative enveloped virus fusogenic peptide domain variant
include, e.g., conservative influenzavirus fusogenic peptide domain
variants, conservative alphavirus fusogenic peptide domain
variants, conservative vesiculovirus fusogenic peptide domain
variants, conservative respirovirus fusogenic peptide domain
variants, conservative morbillivirus fusogenic peptide domain
variants, conservative avulavirus fusogenic peptide domain
variants, conservative henipavirus fusogenic peptide domain
variants, conservative metapneumovirus fusogenic peptide domain
variants and conservative foamy virus fusogenic peptide domain
variants.
[0177] As used herein, the term "non-conservative enveloped virus
fusogenic peptide domain variant" means a enveloped virus fusogenic
peptide domain in which 1) at least one amino acid is deleted from
the reference enveloped virus fusogenic peptide domain on which the
non-conservative enveloped virus fusogenic peptide domain variant
is based; 2) at least one amino acid added to the reference
enveloped virus fusogenic peptide domain on which the
non-conservative enveloped virus fusogenic peptide domain is based;
or 3) at least one amino acid is substituted by another amino acid
or an amino acid analog that does not share any property similar to
that of the original amino acid from the reference enveloped virus
fusogenic peptide domain sequence. A non-conservative enveloped
virus fusogenic peptide domain variant can function in
substantially the same manner as the reference enveloped virus
fusogenic peptide domain on which the non-conservative enveloped
virus fusogenic peptide domain variant is based, and can be
substituted for the reference enveloped virus fusogenic peptide
domain in any aspect of the present invention. A non-conservative
enveloped virus fusogenic peptide domain variant can delete one or
more amino acids, two or more amino acids, three or more amino
acids, four or more amino acids or five or more amino acids from
the reference enveloped virus fusogenic peptide domain on which the
non-conservative enveloped virus fusogenic peptide domain variant
is based. A non-conservative enveloped virus fusogenic peptide
domain variant can add one or more amino acids, two or more amino
acids, three or more amino acids, four or more amino acids or five
or more amino acids to the reference enveloped virus fusogenic
peptide domain on which the non-conservative enveloped virus
fusogenic peptide domain variant is based. A non-conservative
enveloped virus fusogenic peptide domain variant may substitute one
or more amino acids, two or more amino acids, three or more amino
acids, four or more amino acids, five or more amino acids or ten or
more amino acids from the reference enveloped virus fusogenic
peptide domain on which the non-conservative enveloped virus
fusogenic peptide domain variant is based. A non-conservative
enveloped virus fusogenic peptide domain variant can also
substitute at least 2 contiguous amino acids, at least 3 contiguous
amino acids, at least 4 contiguous amino acids or at least 5
contiguous amino acids from the reference enveloped virus fusogenic
peptide domain on which the non-conservative enveloped virus
fusogenic peptide domain variant is based, that possess at least
50% amino acid identity, 65% amino acid identity, 75% amino acid
identity, 85% amino acid identity or 95% amino acid identity to the
reference enveloped virus fusogenic peptide domain on which the
non-conservative enveloped virus fusogenic peptide domain variant
is based. Non-limiting examples of a non-conservative enveloped
virus fusogenic peptide domain variant include, e.g.,
non-conservative influenzavirus fusogenic peptide domain variants,
non-conservative alphavirus fusogenic peptide domain variants,
non-conservative vesiculovirus fusogenic peptide domain variants,
non-conservative respirovirus fusogenic peptide domain variants,
non-conservative morbillivirus fusogenic peptide domain variants,
non-conservative avulavirus fusogenic peptide domain variants,
non-conservative henipavirus fusogenic peptide domain variants,
non-conservative metapneumovirus fusogenic peptide domain variants
and non-conservative foamy virus fusogenic peptide domain
variants.
[0178] As used herein, the term "enveloped virus fusogenic peptide
domain chimeric" means a polypeptide comprising at least a portion
of an enveloped virus fusogenic peptide domain and at least a
portion of at least one other polypeptide to form an enveloped
virus fusogenic peptide domain with at least one property different
from the reference enveloped virus fusogenic peptide domain, with
the proviso that this enveloped virus fusogenic peptide domain
chimeric is still capable of further facilitating the translocation
step of the intoxication process where the LC is released from
intracellular vesicles into the cytoplasm of the target cell and
thus participate in executing the overall cellular mechanism
whereby a Clostridial toxin proteolytically cleaves a
substrate.
[0179] As used herein, the term "active enveloped virus fusogenic
peptide domain fragment" means any of a variety of enveloped virus
fusogenic peptide domain fragments that can further facilitate the
translocation step of the intoxication process where the LC is
released from intracellular vesicles into the cytoplasm of the
target cell and thus participate in executing the overall cellular
mechanism whereby a Clostridial toxin proteolytically cleaves a
substrate. Enveloped virus fusogenic peptide domains are
approximately 15-30 amino acids in length. Thus, aspects of this
embodiment can include a translocation facilitating domain
comprising an active enveloped virus fusogenic peptide domain
fragment having a length of, e.g., at least 10 amino acids, at
least 15 amino acids, at least 20 amino acids and at least 25 amino
acids. Other aspects of this embodiment can include a translocation
facilitating domain comprising an active enveloped virus fusogenic
peptide domain fragment having a length of, e.g., at most 10 amino
acids, at most 15 amino acids, at most 20 amino acids and at most
25 amino acids.
[0180] Any of a variety of sequence alignment methods can be used
to determine percent identity of naturally-occurring enveloped
virus fusogenic peptide domain variants and non-naturally-occurring
enveloped virus fusogenic peptide domain variants, including,
without limitation, global methods, local methods and hybrid
methods, such as, e.g., segment approach methods. Protocols to
determine percent identity are routine procedures within the scope
of one skilled in the art and from the teaching herein.
[0181] Thus, in an embodiment, a modified Clostridial toxin
disclosed in the present specification comprises a Clostridial
toxin translocation facilitating domain comprising an enveloped
virus fusogenic peptide domain. In an aspect of this embodiment, a
Clostridial toxin translocation facilitating domain comprises a
naturally occurring enveloped virus fusogenic peptide domain
variant, such as, e.g., a enveloped virus fusogenic peptide domain
isoform or a enveloped virus fusogenic peptide domain subtype. In
another aspect of this embodiment, a Clostridial toxin
translocation domain comprises a non-naturally occurring enveloped
virus fusogenic peptide domain variant, such as, e.g., a
conservative enveloped virus fusogenic peptide domain variant, a
non-conservative enveloped virus fusogenic peptide domain variant,
a enveloped virus fusogenic peptide domain chimeric, an active
enveloped virus fusogenic peptide domain fragment, or any
combination thereof.
[0182] In another embodiment, a Clostridial toxin translocation
facilitating domain comprises an influenzavirus fusogenic peptide
domain. In another aspect of this embodiment, an influenzavirus
fusogenic peptide domain comprises a naturally occurring
influenzavirus fusogenic peptide domain variant, such as, e.g., an
influenzavirus fusogenic peptide domain isoform or an
influenzavirus fusogenic peptide domain subtype. In another aspect
of this embodiment, an influenzavirus fusogenic peptide domain
comprises a naturally occurring influenzavirus fusogenic peptide
domain variant of SEQ ID NO: 87, SEQ ID NO: 88, SEQ ID NO: 89, SEQ
ID NO: 90 or SEQ ID NO: 91, such as, e.g., an influenzavirus
fusogenic peptide domain isoform of SEQ ID NO: 87, SEQ ID NO: 88,
SEQ ID NO: 89, SEQ ID NO: 90 or SEQ ID NO: 91 or an influenzavirus
fusogenic peptide domain subtype of SEQ ID NO: 87, SEQ ID NO: 88,
SEQ ID NO: 89, SEQ ID NO: 90 or SEQ ID NO: 91. In still another
aspect of this embodiment, an influenzavirus fusogenic peptide
domain comprises a non-naturally occurring influenzavirus fusogenic
peptide domain variant, such as, e.g., a conservative
influenzavirus fusogenic peptide domain variant, a non-conservative
influenzavirus fusogenic peptide domain variant, an influenzavirus
fusogenic peptide domain chimeric, an active influenzavirus
fusogenic peptide domain fragment, or any combination thereof. In
still another aspect of this embodiment, an influenzavirus
fusogenic peptide domain comprises amino acids a non-naturally
occurring influenzavirus fusogenic peptide domain variant of SEQ ID
NO: 87, SEQ ID NO: 88, SEQ ID NO: 89, SEQ ID NO: 90 or SEQ ID NO:
91, such as, e.g., a conservative influenzavirus fusogenic peptide
domain variant of SEQ ID NO: 87, SEQ ID NO: 88, SEQ ID NO: 89, SEQ
ID NO: 90 or SEQ ID NO: 91, a non-conservative influenzavirus
fusogenic peptide domain variant of SEQ ID NO: 87, SEQ ID NO: 88,
SEQ ID NO: 89, SEQ ID NO: 90 or SEQ ID NO: 91, an active
influenzavirus fusogenic peptide domain fragment of SEQ ID NO: 87,
SEQ ID NO: 88, SEQ ID NO: 89, SEQ ID NO: 90 or SEQ ID NO: 91, or
any combination thereof.
[0183] In other aspects of this embodiment, an influenzavirus
fusogenic peptide domain comprises a polypeptide having, e.g., at
least 70% amino acid identity with SEQ ID NO: 87, SEQ ID NO: 88,
SEQ ID NO: 89, SEQ ID NO: 90 or SEQ ID NO: 91, at least 75% amino
acid identity with SEQ ID NO: 87, SEQ ID NO: 88, SEQ ID NO: 89, SEQ
ID NO: 90 or SEQ ID NO: 91, at least 80% amino acid identity with
SEQ ID NO: 87, SEQ ID NO: 88, SEQ ID NO: 89, SEQ ID NO: 90 or SEQ
ID NO: 91, at least 85% amino acid identity with SEQ ID NO: 87, SEQ
ID NO: 88, SEQ ID NO: 89, SEQ ID NO: 90 or SEQ ID NO: 91, at least
90% amino acid identity with SEQ ID NO: 87, SEQ ID NO: 88, SEQ ID
NO: 89, SEQ ID NO: 90 or SEQ ID NO: 91 or at least 95% amino acid
identity with SEQ ID NO: 87, SEQ ID NO: 88, SEQ ID NO: 89, SEQ ID
NO: 90 or SEQ ID NO: 91. In yet other aspects of this embodiment,
an influenzavirus fusogenic peptide domain comprises a polypeptide
having, e.g., at most 70% amino acid identity with SEQ ID NO: 87,
SEQ ID NO: 88, SEQ ID NO: 89, SEQ ID NO: 90 or SEQ ID NO: 91, at
most 75% amino acid identity with SEQ ID NO: 87, SEQ ID NO: 88, SEQ
ID NO: 89, SEQ ID NO: 90 or SEQ ID NO: 91, at most 80% amino acid
identity with SEQ ID NO: 87, SEQ ID NO: 88, SEQ ID NO: 89, SEQ ID
NO: 90 or SEQ ID NO: 91, at most 85% amino acid identity with SEQ
ID NO: 87, SEQ ID NO: 88, SEQ ID NO: 89, SEQ ID NO: 90 or SEQ ID
NO: 91, at most 90% amino acid identity with SEQ ID NO: 87, SEQ ID
NO: 88, SEQ ID NO: 89, SEQ ID NO: 90 or SEQ ID NO: 91 or at most
95% amino acid identity with SEQ ID NO: 87, SEQ ID NO: 88, SEQ ID
NO: 89, SEQ ID NO: 90 or SEQ ID NO: 91.
[0184] In other aspects of this embodiment, an influenzavirus
fusogenic peptide domain comprises a polypeptide having, e.g., at
most one, two, three, four, five, six, seven, eight, nine or 10
non-contiguous amino acid substitutions relative to SEQ ID NO: 87,
SEQ ID NO: 88, SEQ ID NO: 89, SEQ ID NO: 90 or SEQ ID NO: 91. In
other aspects of this embodiment, an influenzavirus fusogenic
peptide domain comprises a polypeptide having, e.g., at least one,
two, three, four, five, six, seven, eight, nine or 10
non-contiguous amino acid substitutions relative to SEQ ID NO: 87,
SEQ ID NO: 88, SEQ ID NO: 89, SEQ ID NO: 90 or SEQ ID NO: 91. In
yet other aspects of this embodiment, an influenzavirus fusogenic
peptide domain comprises a polypeptide having, e.g., at most one,
two, three, four, five, six, seven, eight, nine or 10
non-contiguous amino acid deletions relative to SEQ ID NO: 87, SEQ
ID NO: 88, SEQ ID NO: 89, SEQ ID NO: 90 or SEQ ID NO: 91. In other
aspects of this embodiment, an influenzavirus fusogenic peptide
domain comprises a polypeptide having, e.g., at least one, two,
three, four, five, six, seven, eight, nine or 10 non-contiguous
amino acid deletions relative to SEQ ID NO: 87, SEQ ID NO: 88, SEQ
ID NO: 89, SEQ ID NO: 90 or SEQ ID NO: 91. In still other aspects
of this embodiment, an influenzavirus fusogenic peptide domain
comprises a polypeptide having, e.g., at most one, two, three,
four, five, six, seven, eight, nine or 10 non-contiguous amino acid
additions relative to SEQ ID NO: 87, SEQ ID NO: 88, SEQ ID NO: 89,
SEQ ID NO: 90 or SEQ ID NO: 91. In other aspects of this
embodiment, an influenzavirus fusogenic peptide domain comprises a
polypeptide having, e.g., at least one, two, three, four, five,
six, seven, eight, nine or 10 non-contiguous amino acid additions
relative to SEQ ID NO: 87, SEQ ID NO: 88, SEQ ID NO: 89, SEQ ID NO:
90 or SEQ ID NO: 91.
[0185] In other aspects of this embodiment, an influenzavirus
fusogenic peptide domain comprises a polypeptide having, e.g., at
most one, two, three, four, five, six, seven, eight, nine or 10
contiguous amino acid substitutions relative to SEQ ID NO: 87, SEQ
ID NO: 88, SEQ ID NO: 89, SEQ ID NO: 90 or SEQ ID NO: 91. In other
aspects of this embodiment, an influenzavirus fusogenic peptide
domain comprises a polypeptide having, e.g., at least one, two,
three, four, five, six, seven, eight, nine or 10 contiguous amino
acid substitutions relative to SEQ ID NO: 87, SEQ ID NO: 88, SEQ ID
NO: 89, SEQ ID NO: 90 or SEQ ID NO: 91. In yet other aspects of
this embodiment, an influenzavirus fusogenic peptide domain
comprises a polypeptide having, e.g., at most one, two, three,
four, five, six, seven, eight, nine or 10 contiguous amino acid
deletions relative to SEQ ID NO: 87, SEQ ID NO: 88, SEQ ID NO: 89,
SEQ ID NO: 90 or SEQ ID NO: 91. In other aspects of this
embodiment, an influenzavirus fusogenic peptide domain comprises a
polypeptide having, e.g., at least one, two, three, four, five,
six, seven, eight, nine or 10 contiguous amino acid deletions
relative to SEQ ID NO: 87, SEQ ID NO: 88, SEQ ID NO: 89, SEQ ID NO:
90 or SEQ ID NO: 91. In still other aspects of this embodiment, an
influenzavirus fusogenic peptide domain comprises a polypeptide
having, e.g., at most one, two, three, four, five, six, seven,
eight, nine or 10 contiguous amino acid additions relative to SEQ
ID NO: 87, SEQ ID NO: 88, SEQ ID NO: 89, SEQ ID NO: 90 or SEQ ID
NO: 91. In other aspects of this embodiment, an influenzavirus
fusogenic peptide domain comprises a polypeptide having, e.g., at
least one, two, three, four, five, six, seven, eight, nine or 10
contiguous amino acid additions relative to SEQ ID NO: 87, SEQ ID
NO: 88, SEQ ID NO: 89, SEQ ID NO: 90 or SEQ ID NO: 91.
[0186] In another embodiment, a Clostridial toxin translocation
facilitating domain comprises an alphavirus fusogenic peptide
domain. In another aspect of this embodiment, an alphavirus
fusogenic peptide domain comprises a naturally occurring alphavirus
fusogenic peptide domain variant, such as, e.g., an alphavirus
fusogenic peptide domain isoform or an alphavirus fusogenic peptide
domain subtype. In another aspect of this embodiment, an alphavirus
fusogenic peptide domain comprises a naturally occurring alphavirus
fusogenic peptide domain variant of SEQ ID NO: 92, SEQ ID NO: 93,
SEQ ID NO: 94, SEQ ID NO: 95, SEQ ID NO: 96, SEQ ID NO: 97, SEQ ID
NO: 98, SEQ ID NO: 99, SEQ ID NO: 100, SEQ ID NO: 101, SEQ ID NO:
102, SEQ ID NO: 103, SEQ ID NO: 104, SEQ ID NO: 105, SEQ ID NO:
106, SEQ ID NO: 107, SEQ ID NO: 108, SEQ ID NO: 109, SEQ ID NO:
110, SEQ ID NO: 111, SEQ ID NO: 112, SEQ ID NO: 113, SEQ ID NO:
114, SEQ ID NO: 115, SEQ ID NO: 116, SEQ ID NO: 117 or SEQ ID NO:
118, such as, e.g., an alphavirus fusogenic peptide domain isoform
of SEQ ID NO: 92, SEQ ID NO: 93, SEQ ID NO: 94, SEQ ID NO: 95, SEQ
ID NO: 96, SEQ ID NO: 97, SEQ ID NO: 98, SEQ ID NO: 99, SEQ ID NO:
100, SEQ ID NO: 101, SEQ ID NO: 102, SEQ ID NO: 103, SEQ ID NO:
104, SEQ ID NO: 105, SEQ ID NO: 106, SEQ ID NO: 107, SEQ ID NO:
108, SEQ ID NO: 109, SEQ ID NO: 110, SEQ ID NO: 111, SEQ ID NO:
112, SEQ ID NO: 113, SEQ ID NO: 114, SEQ ID NO: 115, SEQ ID NO:
116, SEQ ID NO: 117 or SEQ ID NO: 118 or an alphavirus fusogenic
peptide domain subtype of SEQ ID NO: 92, SEQ ID NO: 93, SEQ ID NO:
94, SEQ ID NO: 95, SEQ ID NO: 96, SEQ ID NO: 97, SEQ ID NO: 98, SEQ
ID NO: 99, SEQ ID NO: 100, SEQ ID NO: 101, SEQ ID NO: 102, SEQ ID
NO: 103, SEQ ID NO: 104, SEQ ID NO: 105, SEQ ID NO: 106, SEQ ID NO:
107, SEQ ID NO: 108, SEQ ID NO: 109, SEQ ID NO: 110, SEQ ID NO:
111, SEQ ID NO: 112, SEQ ID NO: 113, SEQ ID NO: 114, SEQ ID NO:
115, SEQ ID NO: 116, SEQ ID NO: 117 or SEQ ID NO: 118. In still
another aspect of this embodiment, an alphavirus fusogenic peptide
domain comprises a non-naturally occurring alphavirus fusogenic
peptide domain variant, such as, e.g., a conservative alphavirus
fusogenic peptide domain variant, a non-conservative alphavirus
fusogenic peptide domain variant, an alphavirus fusogenic peptide
domain chimeric, an active alphavirus fusogenic peptide domain
fragment, or any combination thereof. In still another aspect of
this embodiment, an alphavirus fusogenic peptide domain comprises
amino acids a non-naturally occurring alphavirus fusogenic peptide
domain variant of SEQ ID NO: 92, SEQ ID NO: 93, SEQ ID NO: 94, SEQ
ID NO: 95, SEQ ID NO: 96, SEQ ID NO: 97, SEQ ID NO: 98, SEQ ID NO:
99, SEQ ID NO: 100, SEQ ID NO: 101, SEQ ID NO: 102, SEQ ID NO: 103,
SEQ ID NO: 104, SEQ ID NO: 105, SEQ ID NO: 106, SEQ ID NO: 107, SEQ
ID NO: 108, SEQ ID NO: 109, SEQ ID NO: 110, SEQ ID NO: 111, SEQ ID
NO: 112, SEQ ID NO: 113, SEQ ID NO: 114, SEQ ID NO: 115, SEQ ID NO:
116, SEQ ID NO: 117 or SEQ ID NO: 118, such as, e.g., a
conservative alphavirus fusogenic peptide domain variant of SEQ ID
NO: 92, SEQ ID NO: 93, SEQ ID NO: 94, SEQ ID NO: 95, SEQ ID NO: 96,
SEQ ID NO: 97, SEQ ID NO: 98, SEQ ID NO: 99, SEQ ID NO: 100, SEQ ID
NO: 101, SEQ ID NO: 102, SEQ ID NO: 103, SEQ ID NO: 104, SEQ ID NO:
105, SEQ ID NO: 106, SEQ ID NO: 107, SEQ ID NO: 108, SEQ ID NO:
109, SEQ ID NO: 110, SEQ ID NO: 111, SEQ ID NO: 112, SEQ ID NO:
113, SEQ ID NO: 114, SEQ ID NO: 115, SEQ ID NO: 116, SEQ ID NO: 117
or SEQ ID NO: 118, a non-conservative alphavirus fusogenic peptide
domain variant of SEQ ID NO: 92, SEQ ID NO: 93, SEQ ID NO: 94, SEQ
ID NO: 95, SEQ ID NO: 96, SEQ ID NO: 97, SEQ ID NO: 98, SEQ ID NO:
99, SEQ ID NO: 100, SEQ ID NO: 101, SEQ ID NO: 102, SEQ ID NO: 103,
SEQ ID NO: 104, SEQ ID NO: 105, SEQ ID NO: 106, SEQ ID NO: 107, SEQ
ID NO: 108, SEQ ID NO: 109, SEQ ID NO: 110, SEQ ID NO: 111, SEQ ID
NO: 112, SEQ ID NO: 113, SEQ ID NO: 114, SEQ ID NO: 115, SEQ ID NO:
116, SEQ ID NO: 117 or SEQ ID NO: 118, an active alphavirus
fusogenic peptide domain fragment of SEQ ID NO: 92, SEQ ID NO: 93,
SEQ ID NO: 94, SEQ ID NO: 95, SEQ ID NO: 96, SEQ ID NO: 97, SEQ ID
NO: 98, SEQ ID NO: 99, SEQ ID NO: 100, SEQ ID NO: 101, SEQ ID NO:
102, SEQ ID NO: 103, SEQ ID NO: 104, SEQ ID NO: 105, SEQ ID NO:
106, SEQ ID NO: 107, SEQ ID NO: 108, SEQ ID NO: 109, SEQ ID NO:
110, SEQ ID NO: 111, SEQ ID NO: 112, SEQ ID NO: 113, SEQ ID NO:
114, SEQ ID NO: 115, SEQ ID NO: 116, SEQ ID NO: 117 or SEQ ID NO:
118, or any combination thereof.
[0187] In other aspects of this embodiment, an alphavirus fusogenic
peptide domain comprises a polypeptide having, e.g., at least 70%
amino acid identity with SEQ ID NO: 92, SEQ ID NO: 93, SEQ ID NO:
94, SEQ ID NO: 95, SEQ ID NO: 96, SEQ ID NO: 97, SEQ ID NO: 98, SEQ
ID NO: 99, SEQ ID NO: 100, SEQ ID NO: 101, SEQ ID NO: 102, SEQ ID
NO: 103, SEQ ID NO: 104, SEQ ID NO: 105, SEQ ID NO: 106, SEQ ID NO:
107, SEQ ID NO: 108, SEQ ID NO: 109, SEQ ID NO: 110, SEQ ID NO:
111, SEQ ID NO: 112, SEQ ID NO: 113, SEQ ID NO: 114, SEQ ID NO:
115, SEQ ID NO: 116, SEQ ID NO: 117 or SEQ ID NO: 118, at least 75%
amino acid identity with SEQ ID NO: 92, SEQ ID NO: 93, SEQ ID NO:
94, SEQ ID NO: 95, SEQ ID NO: 96, SEQ ID NO: 97, SEQ ID NO: 98, SEQ
ID NO: 99, SEQ ID NO: 100, SEQ ID NO: 101, SEQ ID NO: 102, SEQ ID
NO: 103, SEQ ID NO: 104, SEQ ID NO: 105, SEQ ID NO: 106, SEQ ID NO:
107, SEQ ID NO: 108, SEQ ID NO: 109, SEQ ID NO: 110, SEQ ID NO:
111, SEQ ID NO: 112, SEQ ID NO: 113, SEQ ID NO: 114, SEQ ID NO:
115, SEQ ID NO: 116, SEQ ID NO: 117 or SEQ ID NO: 118, at least 80%
amino acid identity with SEQ ID NO: 87, SEQ ID NO: 88, SEQ ID NO:
89, SEQ ID NO: 90 or SEQ ID NO: 91, at least 85% amino acid
identity with SEQ ID NO: 92, SEQ ID NO: 93, SEQ ID NO: 94, SEQ ID
NO: 95, SEQ ID NO: 96, SEQ ID NO: 97, SEQ ID NO: 98, SEQ ID NO: 99,
SEQ ID NO: 100, SEQ ID NO: 101, SEQ ID NO: 102, SEQ ID NO: 103, SEQ
ID NO: 104, SEQ ID NO: 105, SEQ ID NO: 106, SEQ ID NO: 107, SEQ ID
NO: 108, SEQ ID NO: 109, SEQ ID NO: 110, SEQ ID NO: 111, SEQ ID NO:
112, SEQ ID NO: 113, SEQ ID NO: 114, SEQ ID NO: 115, SEQ ID NO:
116, SEQ ID NO: 117 or SEQ ID NO: 118, at least 90% amino acid
identity with SEQ ID NO: 92, SEQ ID NO: 93, SEQ ID NO: 94, SEQ ID
NO: 95, SEQ ID NO: 96, SEQ ID NO: 97, SEQ ID NO: 98, SEQ ID NO: 99,
SEQ ID NO: 100, SEQ ID NO: 101, SEQ ID NO: 102, SEQ ID NO: 103, SEQ
ID NO: 104, SEQ ID NO: 105, SEQ ID NO: 106, SEQ ID NO: 107, SEQ ID
NO: 108, SEQ ID NO: 109, SEQ ID NO: 110, SEQ ID NO: 111, SEQ ID NO:
112, SEQ ID NO: 113, SEQ ID NO: 114, SEQ ID NO: 115, SEQ ID NO:
116, SEQ ID NO: 117 or SEQ ID NO: 118 or at least 95% amino acid
identity with SEQ ID NO: 92, SEQ ID NO: 93, SEQ ID NO: 94, SEQ ID
NO: 95, SEQ ID NO: 96, SEQ ID NO: 97, SEQ ID NO: 98, SEQ ID NO: 99,
SEQ ID NO: 100, SEQ ID NO: 101, SEQ ID NO: 102, SEQ ID NO: 103, SEQ
ID NO: 104, SEQ ID NO: 105, SEQ ID NO: 106, SEQ ID NO: 107, SEQ ID
NO: 108, SEQ ID NO: 109, SEQ ID NO: 110, SEQ ID NO: 111, SEQ ID NO:
112, SEQ ID NO: 113, SEQ ID NO: 114, SEQ ID NO: 115, SEQ ID NO:
116, SEQ ID NO: 117 or SEQ ID NO: 118. In yet other aspects of this
embodiment, an alphavirus fusogenic peptide domain comprises a
polypeptide having, e.g., at most 70% amino acid identity with SEQ
ID NO: 92, SEQ ID NO: 93, SEQ ID NO: 94, SEQ ID NO: 95, SEQ ID NO:
96, SEQ ID NO: 97, SEQ ID NO: 98, SEQ ID NO: 99, SEQ ID NO: 100,
SEQ ID NO: 101, SEQ ID NO: 102, SEQ ID NO: 103, SEQ ID NO: 104, SEQ
ID NO: 105, SEQ ID NO: 106, SEQ ID NO: 107, SEQ ID NO: 108, SEQ ID
NO: 109, SEQ ID NO: 110, SEQ ID NO: 111, SEQ ID NO: 112, SEQ ID NO:
113, SEQ ID NO: 114, SEQ ID NO: 115, SEQ ID NO: 116, SEQ ID NO: 117
or SEQ ID NO: 118, at most 75% amino acid identity with SEQ ID NO:
92, SEQ ID NO: 93, SEQ ID NO: 94, SEQ ID NO: 95, SEQ ID NO: 96, SEQ
ID NO: 97, SEQ ID NO: 98, SEQ ID NO: 99, SEQ ID NO: 100, SEQ ID NO:
101, SEQ ID NO: 102, SEQ ID NO: 103, SEQ ID NO: 104, SEQ ID NO:
105, SEQ ID NO: 106, SEQ ID NO: 107, SEQ ID NO: 108, SEQ ID NO:
109, SEQ ID NO: 110, SEQ ID NO: 111, SEQ ID NO: 112, SEQ ID NO:
113, SEQ ID NO: 114, SEQ ID NO: 115, SEQ ID NO: 116, SEQ ID NO: 117
or SEQ ID NO: 118, at most 80% amino acid identity with SEQ ID NO:
92, SEQ ID NO: 93, SEQ ID NO: 94, SEQ ID NO: 95, SEQ ID NO: 96, SEQ
ID NO: 97, SEQ ID NO: 98, SEQ ID NO: 99, SEQ ID NO: 100, SEQ ID NO:
101, SEQ ID NO: 102, SEQ ID NO: 103, SEQ ID NO: 104, SEQ ID NO:
105, SEQ ID NO: 106, SEQ ID NO: 107, SEQ ID NO: 108, SEQ ID NO:
109, SEQ ID NO: 110, SEQ ID NO: 111, SEQ ID NO: 112, SEQ ID NO:
113, SEQ ID NO: 114, SEQ ID NO: 115, SEQ ID NO: 116, SEQ ID NO: 117
or SEQ ID NO: 118, at most 85% amino acid identity with SEQ ID NO:
92, SEQ ID NO: 93, SEQ ID NO: 94, SEQ ID NO: 95, SEQ ID NO: 96, SEQ
ID NO: 97, SEQ ID NO: 98, SEQ ID NO: 99, SEQ ID NO: 100, SEQ ID NO:
101, SEQ ID NO: 102, SEQ ID NO: 103, SEQ ID NO: 104, SEQ ID NO:
105, SEQ ID NO: 106, SEQ ID NO: 107, SEQ ID NO: 108, SEQ ID NO:
109, SEQ ID NO: 110, SEQ ID NO: 111, SEQ ID NO: 112, SEQ ID NO:
113, SEQ ID NO: 114, SEQ ID NO: 115, SEQ ID NO: 116, SEQ ID NO: 117
or SEQ ID NO: 118, at most 90% amino acid identity with SEQ ID NO:
92, SEQ ID NO: 93, SEQ ID NO: 94, SEQ ID NO: 95, SEQ ID NO: 96, SEQ
ID NO: 97, SEQ ID NO: 98, SEQ ID NO: 99, SEQ ID NO: 100, SEQ ID NO:
101, SEQ ID NO: 102, SEQ ID NO: 103, SEQ ID NO: 104, SEQ ID NO:
105, SEQ ID NO: 106, SEQ ID NO: 107, SEQ ID NO: 108, SEQ ID NO:
109, SEQ ID NO: 110, SEQ ID NO: 111, SEQ ID NO: 112, SEQ ID NO:
113, SEQ ID NO: 114, SEQ ID NO: 115, SEQ ID NO: 116, SEQ ID NO: 117
or SEQ ID NO: 118 or at most 95% amino acid identity with SEQ ID
NO: 92, SEQ ID NO: 93, SEQ ID NO: 94, SEQ ID NO: 95, SEQ ID NO: 96,
SEQ ID NO: 97, SEQ ID NO: 98, SEQ ID NO: 99, SEQ ID NO: 100, SEQ ID
NO: 101, SEQ ID NO: 102, SEQ ID NO: 103, SEQ ID NO: 104, SEQ ID NO:
105, SEQ ID NO: 106, SEQ ID NO: 107, SEQ ID NO: 108, SEQ ID NO:
109, SEQ ID NO: 110, SEQ ID NO: 111, SEQ ID NO: 112, SEQ ID NO:
113, SEQ ID NO: 114, SEQ ID NO: 115, SEQ ID NO: 116, SEQ ID NO: 117
or SEQ ID NO: 118.
[0188] In other aspects of this embodiment, an alphavirus fusogenic
peptide domain comprises a polypeptide having, e.g., at most one,
two, three, four, five, six, seven, eight, nine or 10
non-contiguous amino acid substitutions relative to SEQ ID NO: 92,
SEQ ID NO: 93, SEQ ID NO: 94, SEQ ID NO: 95, SEQ ID NO: 96, SEQ ID
NO: 97, SEQ ID NO: 98, SEQ ID NO: 99, SEQ ID NO: 100, SEQ ID NO:
101, SEQ ID NO: 102, SEQ ID NO: 103, SEQ ID NO: 104, SEQ ID NO:
105, SEQ ID NO: 106, SEQ ID NO: 107, SEQ ID NO: 108, SEQ ID NO:
109, SEQ ID NO: 110, SEQ ID NO: 111, SEQ ID NO: 112, SEQ ID NO:
113, SEQ ID NO: 114, SEQ ID NO: 115, SEQ ID NO: 116, SEQ ID NO: 117
or SEQ ID NO: 118. In other aspects of this embodiment, an
alphavirus fusogenic peptide domain comprises a polypeptide having,
e.g., at least one, two, three, four, five, six, seven, eight, nine
or 10 non-contiguous amino acid substitutions relative to SEQ ID
NO: 92, SEQ ID NO: 93, SEQ ID NO: 94, SEQ ID NO: 95, SEQ ID NO: 96,
SEQ ID NO: 97, SEQ ID NO: 98, SEQ ID NO: 99, SEQ ID NO: 100, SEQ ID
NO: 101, SEQ ID NO: 102, SEQ ID NO: 103, SEQ ID NO: 104, SEQ ID NO:
105, SEQ ID NO: 106, SEQ ID NO: 107, SEQ ID NO: 108, SEQ ID NO:
109, SEQ ID NO: 110, SEQ ID NO: 111, SEQ ID NO: 112, SEQ ID NO:
113, SEQ ID NO: 114, SEQ ID NO: 115, SEQ ID NO: 116, SEQ ID NO: 117
or SEQ ID NO: 118. In yet other aspects of this embodiment, an
alphavirus fusogenic peptide domain comprises a polypeptide having,
e.g., at most one, two, three, four, five, six, seven, eight, nine
or 10 non-contiguous amino acid deletions relative to SEQ ID NO:
92, SEQ ID NO: 93, SEQ ID NO: 94, SEQ ID NO: 95, SEQ ID NO: 96, SEQ
ID NO: 97, SEQ ID NO: 98, SEQ ID NO: 99, SEQ ID NO: 100, SEQ ID NO:
101, SEQ ID NO: 102, SEQ ID NO: 103, SEQ ID NO: 104, SEQ ID NO:
105, SEQ ID NO: 106, SEQ ID NO: 107, SEQ ID NO: 108, SEQ ID NO:
109, SEQ ID NO: 110, SEQ ID NO: 111, SEQ ID NO: 112, SEQ ID NO:
113, SEQ ID NO: 114, SEQ ID NO: 115, SEQ ID NO: 116, SEQ ID NO: 117
or SEQ ID NO: 118. In other aspects of this embodiment, an
alphavirus fusogenic peptide domain comprises a polypeptide having,
e.g., at least one, two, three, four, five, six, seven, eight, nine
or 10 non-contiguous amino acid deletions relative to SEQ ID NO:
92, SEQ ID NO: 93, SEQ ID NO: 94, SEQ ID NO: 95, SEQ ID NO: 96, SEQ
ID NO: 97, SEQ ID NO: 98, SEQ ID NO: 99, SEQ ID NO: 100, SEQ ID NO:
101, SEQ ID NO: 102, SEQ ID NO: 103, SEQ ID NO: 104, SEQ ID NO:
105, SEQ ID NO: 106, SEQ ID NO: 107, SEQ ID NO: 108, SEQ ID NO:
109, SEQ ID NO: 110, SEQ ID NO: 111, SEQ ID NO: 112, SEQ ID NO:
113, SEQ ID NO: 114, SEQ ID NO: 115, SEQ ID NO: 116, SEQ ID NO: 117
or SEQ ID NO: 118. In still other aspects of this embodiment, an
alphavirus fusogenic peptide domain comprises a polypeptide having,
e.g., at most one, two, three, four, five, six, seven, eight, nine
or 10 non-contiguous amino acid additions relative to SEQ ID NO:
92, SEQ ID NO: 93, SEQ ID NO: 94, SEQ ID NO: 95, SEQ ID NO: 96, SEQ
ID NO: 97, SEQ ID NO: 98, SEQ ID NO: 99, SEQ ID NO: 100, SEQ ID NO:
101, SEQ ID NO: 102, SEQ ID NO: 103, SEQ ID NO: 104, SEQ ID NO:
105, SEQ ID NO: 106, SEQ ID NO: 107, SEQ ID NO: 108, SEQ ID NO:
109, SEQ ID NO: 110, SEQ ID NO: 111, SEQ ID NO: 112, SEQ ID NO:
113, SEQ ID NO: 114, SEQ ID NO: 115, SEQ ID NO: 116, SEQ ID NO: 117
or SEQ ID NO: 118. In other aspects of this embodiment, an
alphavirus fusogenic peptide domain comprises a polypeptide having,
e.g., at least one, two, three, four, five, six, seven, eight, nine
or 10 non-contiguous amino acid additions relative to SEQ ID NO:
92, SEQ ID NO: 93, SEQ ID NO: 94, SEQ ID NO: 95, SEQ ID NO: 96, SEQ
ID NO: 97, SEQ ID NO: 98, SEQ ID NO: 99, SEQ ID NO: 100, SEQ ID NO:
101, SEQ ID NO: 102, SEQ ID NO: 103, SEQ ID NO: 104, SEQ ID NO:
105, SEQ ID NO: 106, SEQ ID NO: 107, SEQ ID NO: 108, SEQ ID NO:
109, SEQ ID NO: 110, SEQ ID NO: 111, SEQ ID NO: 112, SEQ ID NO:
113, SEQ ID NO: 114, SEQ ID NO: 115, SEQ ID NO: 116, SEQ ID NO: 117
or SEQ ID NO: 118.
[0189] In other aspects of this embodiment, an alphavirus fusogenic
peptide domain comprises a polypeptide having, e.g., at most one,
two, three, four, five, six, seven, eight, nine or 10 contiguous
amino acid substitutions relative to SEQ ID NO: 92, SEQ ID NO: 93,
SEQ ID NO: 94, SEQ ID NO: 95, SEQ ID NO: 96, SEQ ID NO: 97, SEQ ID
NO: 98, SEQ ID NO: 99, SEQ ID NO: 100, SEQ ID NO: 101, SEQ ID NO:
102, SEQ ID NO: 103, SEQ ID NO: 104, SEQ ID NO: 105, SEQ ID NO:
106, SEQ ID NO: 107, SEQ ID NO: 108, SEQ ID NO: 109, SEQ ID NO:
110, SEQ ID NO: 111, SEQ ID NO: 112, SEQ ID NO: 113, SEQ ID NO:
114, SEQ ID NO: 115, SEQ ID NO: 116, SEQ ID NO: 117 or SEQ ID NO:
118. In other aspects of this embodiment, an alphavirus fusogenic
peptide domain comprises a polypeptide having, e.g., at least one,
two, three, four, five, six, seven, eight, nine or 10 contiguous
amino acid substitutions relative to SEQ ID NO: 92, SEQ ID NO: 93,
SEQ ID NO: 94, SEQ ID NO: 95, SEQ ID NO: 96, SEQ ID NO: 97, SEQ ID
NO: 98, SEQ ID NO: 99, SEQ ID NO: 100, SEQ ID NO: 101, SEQ ID NO:
102, SEQ ID NO: 103, SEQ ID NO: 104, SEQ ID NO: 105, SEQ ID NO:
106, SEQ ID NO: 107, SEQ ID NO: 108, SEQ ID NO: 109, SEQ ID NO:
110, SEQ ID NO: 111, SEQ ID NO: 112, SEQ ID NO: 113, SEQ ID NO:
114, SEQ ID NO: 115, SEQ ID NO: 116, SEQ ID NO: 117 or SEQ ID NO:
118. In yet other aspects of this embodiment, an alphavirus
fusogenic peptide domain comprises a polypeptide having, e.g., at
most one, two, three, four, five, six, seven, eight, nine or 10
contiguous amino acid deletions relative to SEQ ID NO: 92, SEQ ID
NO: 93, SEQ ID NO: 94, SEQ ID NO: 95, SEQ ID NO: 96, SEQ ID NO: 97,
SEQ ID NO: 98, SEQ ID NO: 99, SEQ ID NO: 100, SEQ ID NO: 101, SEQ
ID NO: 102, SEQ ID NO: 103, SEQ ID NO: 104, SEQ ID NO: 105, SEQ ID
NO: 106, SEQ ID NO: 107, SEQ ID NO: 108, SEQ ID NO: 109, SEQ ID NO:
110, SEQ ID NO: 111, SEQ ID NO: 112, SEQ ID NO: 113, SEQ ID NO:
114, SEQ ID NO: 115, SEQ ID NO: 116, SEQ ID NO: 117 or SEQ ID NO:
118. In other aspects of this embodiment, an alphavirus fusogenic
peptide domain comprises a polypeptide having, e.g., at least one,
two, three, four, five, six, seven, eight, nine or 10 contiguous
amino acid deletions relative to SEQ ID NO: 92, SEQ ID NO: 93, SEQ
ID NO: 94, SEQ ID NO: 95, SEQ ID NO: 96, SEQ ID NO: 97, SEQ ID NO:
98, SEQ ID NO: 99, SEQ ID NO: 100, SEQ ID NO: 101, SEQ ID NO: 102,
SEQ ID NO: 103, SEQ ID NO: 104, SEQ ID NO: 105, SEQ ID NO: 106, SEQ
ID NO: 107, SEQ ID NO: 108, SEQ ID NO: 109, SEQ ID NO: 110, SEQ ID
NO: 111, SEQ ID NO: 112, SEQ ID NO: 113, SEQ ID NO: 114, SEQ ID NO:
115, SEQ ID NO: 116, SEQ ID NO: 117 or SEQ ID NO: 118. In still
other aspects of this embodiment, an alphavirus fusogenic peptide
domain comprises a polypeptide having, e.g., at most one, two,
three, four, five, six, seven, eight, nine or 10 contiguous amino
acid additions relative to SEQ ID NO: 92, SEQ ID NO: 93, SEQ ID NO:
94, SEQ ID NO: 95, SEQ ID NO: 96, SEQ ID NO: 97, SEQ ID NO: 98, SEQ
ID NO: 99, SEQ ID NO: 100, SEQ ID NO: 101, SEQ ID NO: 102, SEQ ID
NO: 103, SEQ ID NO: 104, SEQ ID NO: 105, SEQ ID NO: 106, SEQ ID NO:
107, SEQ ID NO: 108, SEQ ID NO: 109, SEQ ID NO: 110, SEQ ID NO:
111, SEQ ID NO: 112, SEQ ID NO: 113, SEQ ID NO: 114, SEQ ID NO:
115, SEQ ID NO: 116, SEQ ID NO: 117 or SEQ ID NO: 118. In other
aspects of this embodiment, an alphavirus fusogenic peptide domain
comprises a polypeptide having, e.g., at least one, two, three,
four, five, six, seven, eight, nine or 10 contiguous amino acid
additions relative to SEQ ID NO: 92, SEQ ID NO: 93, SEQ ID NO: 94,
SEQ ID NO: 95, SEQ ID NO: 96, SEQ ID NO: 97, SEQ ID NO: 98, SEQ ID
NO: 99, SEQ ID NO: 100, SEQ ID NO: 101, SEQ ID NO: 102, SEQ ID NO:
103, SEQ ID NO: 104, SEQ ID NO: 105, SEQ ID NO: 106, SEQ ID NO:
107, SEQ ID NO: 108, SEQ ID NO: 109, SEQ ID NO: 110, SEQ ID NO:
111, SEQ ID NO: 112, SEQ ID NO: 113, SEQ ID NO: 114, SEQ ID NO:
115, SEQ ID NO: 116, SEQ ID NO: 117 or SEQ ID NO: 118.
[0190] In another embodiment, a Clostridial toxin translocation
facilitating domain comprises a vesiculovirus fusogenic peptide
domain. In another aspect of this embodiment, a vesiculovirus
fusogenic peptide domain comprises a naturally occurring
vesiculovirus fusogenic peptide domain variant, such as, e.g., a
vesiculovirus fusogenic peptide domain isoform or a vesiculovirus
fusogenic peptide domain subtype. In another aspect of this
embodiment, a vesiculovirus fusogenic peptide domain comprises a
naturally occurring vesiculovirus fusogenic peptide domain variant
of SEQ ID NO: 119, SEQ ID NO: 120, SEQ ID NO: 121, SEQ ID NO: 122,
SEQ ID NO: 123, SEQ ID NO: 124, SEQ ID NO: 125, SEQ ID NO: 126, SEQ
ID NO: 127, SEQ ID NO: 128 or SEQ ID NO: 129, such as, e.g., a
vesiculovirus fusogenic peptide domain isoform of SEQ ID NO: 119,
SEQ ID NO: 120, SEQ ID NO: 121, SEQ ID NO: 122, SEQ ID NO: 123, SEQ
ID NO: 124, SEQ ID NO: 125, SEQ ID NO: 126, SEQ ID NO: 127, SEQ ID
NO: 128 or SEQ ID NO: 129 or a vesiculovirus fusogenic peptide
domain subtype of SEQ ID NO: 119, SEQ ID NO: 120, SEQ ID NO: 121,
SEQ ID NO: 122, SEQ ID NO: 123, SEQ ID NO: 124, SEQ ID NO: 125, SEQ
ID NO: 126, SEQ ID NO: 127, SEQ ID NO: 128 or SEQ ID NO: 129. In
still another aspect of this embodiment, a vesiculovirus fusogenic
peptide domain comprises a non-naturally occurring vesiculovirus
fusogenic peptide domain variant, such as, e.g., a conservative
vesiculovirus fusogenic peptide domain variant, a non-conservative
vesiculovirus fusogenic peptide domain variant, a vesiculovirus
fusogenic peptide domain chimeric, an active vesiculovirus
fusogenic peptide domain fragment, or any combination thereof. In
still another aspect of this embodiment, a vesiculovirus fusogenic
peptide domain comprises amino acids a non-naturally occurring
vesiculovirus fusogenic peptide domain variant of SEQ ID NO: 119,
SEQ ID NO: 120, SEQ ID NO: 121, SEQ ID NO: 122, SEQ ID NO: 123, SEQ
ID NO: 124, SEQ ID NO: 125, SEQ ID NO: 126, SEQ ID NO: 127, SEQ ID
NO: 128 or SEQ ID NO: 129, such as, e.g., a conservative
vesiculovirus fusogenic peptide domain variant of SEQ ID NO: 119,
SEQ ID NO: 120, SEQ ID NO: 121, SEQ ID NO: 122, SEQ ID NO: 123, SEQ
ID NO: 124, SEQ ID NO: 125, SEQ ID NO: 126, SEQ ID NO: 127, SEQ ID
NO: 128 or SEQ ID NO: 129, a non-conservative vesiculovirus
fusogenic peptide domain variant of SEQ ID NO: 119, SEQ ID NO: 120,
SEQ ID NO: 121, SEQ ID NO: 122, SEQ ID NO: 123, SEQ ID NO: 124, SEQ
ID NO: 125, SEQ ID NO: 126, SEQ ID NO: 127, SEQ ID NO: 128 or SEQ
ID NO: 129, an active vesiculovirus fusogenic peptide domain
fragment of SEQ ID NO: 119, SEQ ID NO: 120, SEQ ID NO: 121, SEQ ID
NO: 122, SEQ ID NO: 123, SEQ ID NO: 124, SEQ ID NO: 125, SEQ ID NO:
126, SEQ ID NO: 127, SEQ ID NO: 128 or SEQ ID NO: 129, or any
combination thereof.
[0191] In other aspects of this embodiment, a vesiculovirus
fusogenic peptide domain comprises a polypeptide having, e.g., at
least 70% amino acid identity with SEQ ID NO: 119, SEQ ID NO: 120,
SEQ ID NO: 121, SEQ ID NO: 122, SEQ ID NO: 123, SEQ ID NO: 124, SEQ
ID NO: 125, SEQ ID NO: 126, SEQ ID NO: 127, SEQ ID NO: 128 or SEQ
ID NO: 129, at least 75% amino acid identity with SEQ ID NO: 119,
SEQ ID NO: 120, SEQ ID NO: 121, SEQ ID NO: 122, SEQ ID NO: 123, SEQ
ID NO: 124, SEQ ID NO: 125, SEQ ID NO: 126, SEQ ID NO: 127, SEQ ID
NO: 128 or SEQ ID NO: 129, at least 80% amino acid identity with
SEQ ID NO: 119, SEQ ID NO: 120, SEQ ID NO: 121, SEQ ID NO: 122, SEQ
ID NO: 123, SEQ ID NO: 124, SEQ ID NO: 125, SEQ ID NO: 126, SEQ ID
NO: 127, SEQ ID NO: 128 or SEQ ID NO: 129, at least 85% amino acid
identity with SEQ ID NO: 119, SEQ ID NO: 120, SEQ ID NO: 121, SEQ
ID NO: 122, SEQ ID NO: 123, SEQ ID NO: 124, SEQ ID NO: 125, SEQ ID
NO: 126, SEQ ID NO: 127, SEQ ID NO: 128 or SEQ ID NO: 129, at least
90% amino acid identity with SEQ ID NO: 119, SEQ ID NO: 120, SEQ ID
NO: 121, SEQ ID NO: 122, SEQ ID NO: 123, SEQ ID NO: 124, SEQ ID NO:
125, SEQ ID NO: 126, SEQ ID NO: 127, SEQ ID NO: 128 or SEQ ID NO:
129 or at least 95% amino acid identity with SEQ ID NO: 119, SEQ ID
NO: 120, SEQ ID NO: 121, SEQ ID NO: 122, SEQ ID NO: 123, SEQ ID NO:
124, SEQ ID NO: 125, SEQ ID NO: 126, SEQ ID NO: 127, SEQ ID NO: 128
or SEQ ID NO: 129. In yet other aspects of this embodiment, a
vesiculovirus fusogenic peptide domain comprises a polypeptide
having, e.g., at most 70% amino acid identity with SEQ ID NO: 119,
SEQ ID NO: 120, SEQ ID NO: 121, SEQ ID NO: 122, SEQ ID NO: 123, SEQ
ID NO: 124, SEQ ID NO: 125, SEQ ID NO: 126, SEQ ID NO: 127, SEQ ID
NO: 128 or SEQ ID NO: 129, at most 75% amino acid identity with SEQ
ID NO: 119, SEQ ID NO: 120, SEQ ID NO: 121, SEQ ID NO: 122, SEQ ID
NO: 123, SEQ ID NO: 124, SEQ ID NO: 125, SEQ ID NO: 126, SEQ ID NO:
127, SEQ ID NO: 128 or SEQ ID NO: 129, at most 80% amino acid
identity with SEQ ID NO: 119, SEQ ID NO: 120, SEQ ID NO: 121, SEQ
ID NO: 122, SEQ ID NO: 123, SEQ ID NO: 124, SEQ ID NO: 125, SEQ ID
NO: 126, SEQ ID NO: 127, SEQ ID NO: 128 or SEQ ID NO: 129, at most
85% amino acid identity with SEQ ID NO: 119, SEQ ID NO: 120, SEQ ID
NO: 121, SEQ ID NO: 122, SEQ ID NO: 123, SEQ ID NO: 124, SEQ ID NO:
125, SEQ ID NO: 126, SEQ ID NO: 127, SEQ ID NO: 128 or SEQ ID NO:
129, at most 90% amino acid identity with SEQ ID NO: 119, SEQ ID
NO: 120, SEQ ID NO: 121, SEQ ID NO: 122, SEQ ID NO: 123, SEQ ID NO:
124, SEQ ID NO: 125, SEQ ID NO: 126, SEQ ID NO: 127, SEQ ID NO: 128
or SEQ ID NO: 129 or at most 95% amino acid identity with SEQ ID
NO: 119, SEQ ID NO: 120, SEQ ID NO: 121, SEQ ID NO: 122, SEQ ID NO:
123, SEQ ID NO: 124, SEQ ID NO: 125, SEQ ID NO: 126, SEQ ID NO:
127, SEQ ID NO: 128 or SEQ ID NO: 129.
[0192] In other aspects of this embodiment, a vesiculovirus
fusogenic peptide domain comprises a polypeptide having, e.g., at
most one, two, three, four, five, six, seven, eight, nine or 10
non-contiguous amino acid substitutions relative to SEQ ID NO: 119,
SEQ ID NO: 120, SEQ ID NO: 121, SEQ ID NO: 122, SEQ ID NO: 123, SEQ
ID NO: 124, SEQ ID NO: 125, SEQ ID NO: 126, SEQ ID NO: 127, SEQ ID
NO: 128 or SEQ ID NO: 129. In other aspects of this embodiment, a
vesiculovirus fusogenic peptide domain comprises a polypeptide
having, e.g., at least one, two, three, four, five, six, seven,
eight, nine or 10 non-contiguous amino acid substitutions relative
to SEQ ID NO: 119, SEQ ID NO: 120, SEQ ID NO: 121, SEQ ID NO: 122,
SEQ ID NO: 123, SEQ ID NO: 124, SEQ ID NO: 125, SEQ ID NO: 126, SEQ
ID NO: 127, SEQ ID NO: 128 or SEQ ID NO: 129. In yet other aspects
of this embodiment, a vesiculovirus fusogenic peptide domain
comprises a polypeptide having, e.g., at most one, two, three,
four, five, six, seven, eight, nine or 10 non-contiguous amino acid
deletions relative to SEQ ID NO: 119, SEQ ID NO: 120, SEQ ID NO:
121, SEQ ID NO: 122, SEQ ID NO: 123, SEQ ID NO: 124, SEQ ID NO:
125, SEQ ID NO: 126, SEQ ID NO: 127, SEQ ID NO: 128 or SEQ ID NO:
129. In other aspects of this embodiment, a vesiculovirus fusogenic
peptide domain comprises a polypeptide having, e.g., at least one,
two, three, four, five, six, seven, eight, nine or 10
non-contiguous amino acid deletions relative to SEQ ID NO: 119, SEQ
ID NO: 120, SEQ ID NO: 121, SEQ ID NO: 122, SEQ ID NO: 123, SEQ ID
NO: 124, SEQ ID NO: 125, SEQ ID NO: 126, SEQ ID NO: 127, SEQ ID NO:
128 or SEQ ID NO: 129. In still other aspects of this embodiment, a
vesiculovirus fusogenic peptide domain comprises a polypeptide
having, e.g., at most one, two, three, four, five, six, seven,
eight, nine or 10 non-contiguous amino acid additions relative to
SEQ ID NO: 119, SEQ ID NO: 120, SEQ ID NO: 121, SEQ ID NO: 122, SEQ
ID NO: 123, SEQ ID NO: 124, SEQ ID NO: 125, SEQ ID NO: 126, SEQ ID
NO: 127, SEQ ID NO: 128 or SEQ ID NO: 129. In other aspects of this
embodiment, a vesiculovirus fusogenic peptide domain comprises a
polypeptide having, e.g., at least one, two, three, four, five,
six, seven, eight, nine or 10 non-contiguous amino acid additions
relative to SEQ ID NO: 119, SEQ ID NO: 120, SEQ ID NO: 121, SEQ ID
NO: 122, SEQ ID NO: 123, SEQ ID NO: 124, SEQ ID NO: 125, SEQ ID NO:
126, SEQ ID NO: 127, SEQ ID NO: 128 or SEQ ID NO: 129.
[0193] In other aspects of this embodiment, a vesiculovirus
fusogenic peptide domain comprises a polypeptide having, e.g., at
most one, two, three, four, five, six, seven, eight, nine or 10
contiguous amino acid substitutions relative to SEQ ID NO: 119, SEQ
ID NO: 120, SEQ ID NO: 121, SEQ ID NO: 122, SEQ ID NO: 123, SEQ ID
NO: 124, SEQ ID NO: 125, SEQ ID NO: 126, SEQ ID NO: 127, SEQ ID NO:
128 or SEQ ID NO: 129. In other aspects of this embodiment, a
vesiculovirus fusogenic peptide domain comprises a polypeptide
having, e.g., at least one, two, three, four, five, six, seven,
eight, nine or 10 contiguous amino acid substitutions relative to
SEQ ID NO: 119, SEQ ID NO: 120, SEQ ID NO: 121, SEQ ID NO: 122, SEQ
ID NO: 123, SEQ ID NO: 124, SEQ ID NO: 125, SEQ ID NO: 126, SEQ ID
NO: 127, SEQ ID NO: 128 or SEQ ID NO: 129. In yet other aspects of
this embodiment, a vesiculovirus fusogenic peptide domain comprises
a polypeptide having, e.g., at most one, two, three, four, five,
six, seven, eight, nine or 10 contiguous amino acid deletions
relative to SEQ ID NO: 119, SEQ ID NO: 120, SEQ ID NO: 121, SEQ ID
NO: 122, SEQ ID NO: 123, SEQ ID NO: 124, SEQ ID NO: 125, SEQ ID NO:
126, SEQ ID NO: 127, SEQ ID NO: 128 or SEQ ID NO: 129. In other
aspects of this embodiment, a vesiculovirus fusogenic peptide
domain comprises a polypeptide having, e.g., at least one, two,
three, four, five, six, seven, eight, nine or 10 contiguous amino
acid deletions relative to SEQ ID NO: 119, SEQ ID NO: 120, SEQ ID
NO: 121, SEQ ID NO: 122, SEQ ID NO: 123, SEQ ID NO: 124, SEQ ID NO:
125, SEQ ID NO: 126, SEQ ID NO: 127, SEQ ID NO: 128 or SEQ ID NO:
129. In still other aspects of this embodiment, a vesiculovirus
fusogenic peptide domain comprises a polypeptide having, e.g., at
most one, two, three, four, five, six, seven, eight, nine or 10
contiguous amino acid additions relative to SEQ ID NO: 119, SEQ ID
NO: 120, SEQ ID NO: 121, SEQ ID NO: 122, SEQ ID NO: 123, SEQ ID NO:
124, SEQ ID NO: 125, SEQ ID NO: 126, SEQ ID NO: 127, SEQ ID NO: 128
or SEQ ID NO: 129. In other aspects of this embodiment, a
vesiculovirus fusogenic peptide domain comprises a polypeptide
having, e.g., at least one, two, three, four, five, six, seven,
eight, nine or 10 contiguous amino acid additions relative to SEQ
ID NO: 119, SEQ ID NO: 120, SEQ ID NO: 121, SEQ ID NO: 122, SEQ ID
NO: 123, SEQ ID NO: 124, SEQ ID NO: 125, SEQ ID NO: 126, SEQ ID NO:
127, SEQ ID NO: 128 or SEQ ID NO: 129.
[0194] In another embodiment, a Clostridial toxin translocation
facilitating domain comprises a respirovirus fusogenic peptide
domain. In another aspect of this embodiment, a respirovirus
fusogenic peptide domain comprises a naturally occurring
respirovirus fusogenic peptide domain variant, such as, e.g., a
respirovirus fusogenic peptide domain isoform or a respirovirus
fusogenic peptide domain subtype. In another aspect of this
embodiment, a respirovirus fusogenic peptide domain comprises a
naturally occurring respirovirus fusogenic peptide domain variant
of SEQ ID NO: 130, SEQ ID NO: 131, SEQ ID NO: 132, SEQ ID NO: 133,
SEQ ID NO: 134, SEQ ID NO: 135 or SEQ ID NO: 136, such as, e.g., a
respirovirus fusogenic peptide domain isoform of SEQ ID NO: 130,
SEQ ID NO: 131, SEQ ID NO: 132, SEQ ID NO: 133, SEQ ID NO: 134, SEQ
ID NO: 135 or SEQ ID NO: 136 or a respirovirus fusogenic peptide
domain subtype of SEQ ID NO: 130, SEQ ID NO: 131, SEQ ID NO: 132,
SEQ ID NO: 133, SEQ ID NO: 134, SEQ ID NO: 135 or SEQ ID NO: 136.
In still another aspect of this embodiment, a respirovirus
fusogenic peptide domain comprises a non-naturally occurring
respirovirus fusogenic peptide domain variant, such as, e.g., a
conservative respirovirus fusogenic peptide domain variant, a
non-conservative respirovirus fusogenic peptide domain variant, a
respirovirus fusogenic peptide domain chimeric, an active
respirovirus fusogenic peptide domain fragment, or any combination
thereof. In still another aspect of this embodiment, a respirovirus
fusogenic peptide domain comprises amino acids a non-naturally
occurring respirovirus fusogenic peptide domain variant of SEQ ID
NO: 130, SEQ ID NO: 131, SEQ ID NO: 132, SEQ ID NO: 133, SEQ ID NO:
134, SEQ ID NO: 135 or SEQ ID NO: 136, such as, e.g., a
conservative respirovirus fusogenic peptide domain variant of SEQ
ID NO: 130, SEQ ID NO: 131, SEQ ID NO: 132, SEQ ID NO: 133, SEQ ID
NO: 134, SEQ ID NO: 135 or SEQ ID NO: 136, a non-conservative
respirovirus fusogenic peptide domain variant of SEQ ID NO: 130,
SEQ ID NO: 131, SEQ ID NO: 132, SEQ ID NO: 133, SEQ ID NO: 134, SEQ
ID NO: 135 or SEQ ID NO: 136, an active respirovirus fusogenic
peptide domain fragment of SEQ ID NO: 130, SEQ ID NO: 131, SEQ ID
NO: 132, SEQ ID NO: 133, SEQ ID NO: 134, SEQ ID NO: 135 or SEQ ID
NO: 136, or any combination thereof.
[0195] In other aspects of this embodiment, a respirovirus
fusogenic peptide domain comprises a polypeptide having, e.g., at
least 70% amino acid identity with SEQ ID NO: 130, SEQ ID NO: 131,
SEQ ID NO: 132, SEQ ID NO: 133, SEQ ID NO: 134, SEQ ID NO: 135 or
SEQ ID NO: 136, at least 75% amino acid identity with SEQ ID NO:
130, SEQ ID NO: 131, SEQ ID NO: 132, SEQ ID NO: 133, SEQ ID NO:
134, SEQ ID NO: 135 or SEQ ID NO: 136, at least 80% amino acid
identity with SEQ ID NO: 130, SEQ ID NO: 131, SEQ ID NO: 132, SEQ
ID NO: 133, SEQ ID NO: 134, SEQ ID NO: 135 or SEQ ID NO: 136, at
least 85% amino acid identity with SEQ ID NO: 130, SEQ ID NO: 131,
SEQ ID NO: 132, SEQ ID NO: 133, SEQ ID NO: 134, SEQ ID NO: 135 or
SEQ ID NO: 136, at least 90% amino acid identity with SEQ ID NO:
130, SEQ ID NO: 131, SEQ ID NO: 132, SEQ ID NO: 133, SEQ ID NO:
134, SEQ ID NO: 135 or SEQ ID NO: 136 or at least 95% amino acid
identity with SEQ ID NO: 130, SEQ ID NO: 131, SEQ ID NO: 132, SEQ
ID NO: 133, SEQ ID NO: 134, SEQ ID NO: 135 or SEQ ID NO: 136. In
yet other aspects of this embodiment, a respirovirus fusogenic
peptide domain comprises a polypeptide having, e.g., at most 70%
amino acid identity with SEQ ID NO: 130, SEQ ID NO: 131, SEQ ID NO:
132, SEQ ID NO: 133, SEQ ID NO: 134, SEQ ID NO: 135 or SEQ ID NO:
136, at most 75% amino acid identity with SEQ ID NO: 130, SEQ ID
NO: 131, SEQ ID NO: 132, SEQ ID NO: 133, SEQ ID NO: 134, SEQ ID NO:
135 or SEQ ID NO: 136, at most 80% amino acid identity with SEQ ID
NO: 130, SEQ ID NO: 131, SEQ ID NO: 132, SEQ ID NO: 133, SEQ ID NO:
134, SEQ ID NO: 135 or SEQ ID NO: 136, at most 85% amino acid
identity with SEQ ID NO: 130, SEQ ID NO: 131, SEQ ID NO: 132, SEQ
ID NO: 133, SEQ ID NO: 134, SEQ ID NO: 135 or SEQ ID NO: 136, at
most 90% amino acid identity with SEQ ID NO: 130, SEQ ID NO: 131,
SEQ ID NO: 132, SEQ ID NO: 133, SEQ ID NO: 134, SEQ ID NO: 135 or
SEQ ID NO: 136 or at most 95% amino acid identity with SEQ ID NO:
130, SEQ ID NO: 131, SEQ ID NO: 132, SEQ ID NO: 133, SEQ ID NO:
134, SEQ ID NO: 135 or SEQ ID NO: 136.
[0196] In other aspects of this embodiment, a respirovirus
fusogenic peptide domain comprises a polypeptide having, e.g., at
most one, two, three, four, five, six, seven, eight, nine or 10
non-contiguous amino acid substitutions relative to SEQ ID NO: 130,
SEQ ID NO: 131, SEQ ID NO: 132, SEQ ID NO: 133, SEQ ID NO: 134, SEQ
ID NO: 135 or SEQ ID NO: 136. In other aspects of this embodiment,
a respirovirus fusogenic peptide domain comprises a polypeptide
having, e.g., at least one, two, three, four, five, six, seven,
eight, nine or 10 non-contiguous amino acid substitutions relative
to SEQ ID NO: 130, SEQ ID NO: 131, SEQ ID NO: 132, SEQ ID NO: 133,
SEQ ID NO: 134, SEQ ID NO: 135 or SEQ ID NO: 136. In yet other
aspects of this embodiment, a respirovirus fusogenic peptide domain
comprises a polypeptide having, e.g., at most one, two, three,
four, five, six, seven, eight, nine or 10 non-contiguous amino acid
deletions relative to SEQ ID NO: 130, SEQ ID NO: 131, SEQ ID NO:
132, SEQ ID NO: 133, SEQ ID NO: 134, SEQ ID NO: 135 or SEQ ID NO:
136. In other aspects of this embodiment, a respirovirus fusogenic
peptide domain comprises a polypeptide having, e.g., at least one,
two, three, four, five, six, seven, eight, nine or 10
non-contiguous amino acid deletions relative to SEQ ID NO: 130, SEQ
ID NO: 131, SEQ ID NO: 132, SEQ ID NO: 133, SEQ ID NO: 134, SEQ ID
NO: 135 or SEQ ID NO: 136. In still other aspects of this
embodiment, a respirovirus fusogenic peptide domain comprises a
polypeptide having, e.g., at most one, two, three, four, five, six,
seven, eight, nine or 10 non-contiguous amino acid additions
relative to SEQ ID NO: 130, SEQ ID NO: 131, SEQ ID NO: 132, SEQ ID
NO: 133, SEQ ID NO: 134, SEQ ID NO: 135 or SEQ ID NO: 136. In other
aspects of this embodiment, a respirovirus fusogenic peptide domain
comprises a polypeptide having, e.g., at least one, two, three,
four, five, six, seven, eight, nine or 10 non-contiguous amino acid
additions relative to SEQ ID NO: 130, SEQ ID NO: 131, SEQ ID NO:
132, SEQ ID NO: 133, SEQ ID NO: 134, SEQ ID NO: 135 or SEQ ID NO:
136.
[0197] In other aspects of this embodiment, a respirovirus
fusogenic peptide domain comprises a polypeptide having, e.g., at
most one, two, three, four, five, six, seven, eight, nine or 10
contiguous amino acid substitutions relative to SEQ ID NO: 130, SEQ
ID NO: 131, SEQ ID NO: 132, SEQ ID NO: 133, SEQ ID NO: 134, SEQ ID
NO: 135 or SEQ ID NO: 136. In other aspects of this embodiment, a
respirovirus fusogenic peptide domain comprises a polypeptide
having, e.g., at least one, two, three, four, five, six, seven,
eight, nine or 10 contiguous amino acid substitutions relative to
SEQ ID NO: 130, SEQ ID NO: 131, SEQ ID NO: 132, SEQ ID NO: 133, SEQ
ID NO: 134, SEQ ID NO: 135 or SEQ ID NO: 136. In yet other aspects
of this embodiment, a respirovirus fusogenic peptide domain
comprises a polypeptide having, e.g., at most one, two, three,
four, five, six, seven, eight, nine or 10 contiguous amino acid
deletions relative to SEQ ID NO: 130, SEQ ID NO: 131, SEQ ID NO:
132, SEQ ID NO: 133, SEQ ID NO: 134, SEQ ID NO: 135 or SEQ ID NO:
136. In other aspects of this embodiment, a respirovirus fusogenic
peptide domain comprises a polypeptide having, e.g., at least one,
two, three, four, five, six, seven, eight, nine or 10 contiguous
amino acid deletions relative to SEQ ID NO: 130, SEQ ID NO: 131,
SEQ ID NO: 132, SEQ ID NO: 133, SEQ ID NO: 134, SEQ ID NO: 135 or
SEQ ID NO: 136. In still other aspects of this embodiment, a
respirovirus fusogenic peptide domain comprises a polypeptide
having, e.g., at most one, two, three, four, five, six, seven,
eight, nine or 10 contiguous amino acid additions relative to SEQ
ID NO: 130, SEQ ID NO: 131, SEQ ID NO: 132, SEQ ID NO: 133, SEQ ID
NO: 134, SEQ ID NO: 135 or SEQ ID NO: 136. In other aspects of this
embodiment, a respirovirus fusogenic peptide domain comprises a
polypeptide having, e.g., at least one, two, three, four, five,
six, seven, eight, nine or 10 contiguous amino acid additions
relative to SEQ ID NO: 130, SEQ ID NO: 131, SEQ ID NO: 132, SEQ ID
NO: 133, SEQ ID NO: 134, SEQ ID NO: 135 or SEQ ID NO: 136.
[0198] In another embodiment, a Clostridial toxin translocation
facilitating domain comprises a morbillivirus fusogenic peptide
domain. In another aspect of this embodiment, a morbillivirus
fusogenic peptide domain comprises a naturally occurring
morbillivirus fusogenic peptide domain variant, such as, e.g., a
morbillivirus fusogenic peptide domain isoform or a morbillivirus
fusogenic peptide domain subtype. In another aspect of this
embodiment, a morbillivirus fusogenic peptide domain comprises a
naturally occurring morbillivirus fusogenic peptide domain variant
of SEQ ID NO: 137, SEQ ID NO: 138, SEQ ID NO: 139, SEQ ID NO: 140,
SEQ ID NO: 141, SEQ ID NO: 142, SEQ ID NO: 143, SEQ ID NO: 144, SEQ
ID NO: 145 or SEQ ID NO: 146, such as, e.g., a morbillivirus
fusogenic peptide domain isoform of SEQ ID NO: 137, SEQ ID NO: 138,
SEQ ID NO: 139, SEQ ID NO: 140, SEQ ID NO: 141, SEQ ID NO: 142, SEQ
ID NO: 143, SEQ ID NO: 144, SEQ ID NO: 145 or SEQ ID NO: 146 or a
morbillivirus fusogenic peptide domain subtype of SEQ ID NO: 137,
SEQ ID NO: 138, SEQ ID NO: 139, SEQ ID NO: 140, SEQ ID NO: 141, SEQ
ID NO: 142, SEQ ID NO: 143, SEQ ID NO: 144, SEQ ID NO: 145 or SEQ
ID NO: 146. In still another aspect of this embodiment, a
morbillivirus fusogenic peptide domain comprises a non-naturally
occurring morbillivirus fusogenic peptide domain variant, such as,
e.g., a conservative morbillivirus fusogenic peptide domain
variant, a non-conservative morbillivirus fusogenic peptide domain
variant, a morbillivirus fusogenic peptide domain chimeric, an
active morbillivirus fusogenic peptide domain fragment, or any
combination thereof. In still another aspect of this embodiment, a
morbillivirus fusogenic peptide domain comprises amino acids a
non-naturally occurring morbillivirus fusogenic peptide domain
variant of SEQ ID NO: 137, SEQ ID NO: 138, SEQ ID NO: 139, SEQ ID
NO: 140, SEQ ID NO: 141, SEQ ID NO: 142, SEQ ID NO: 143, SEQ ID NO:
144, SEQ ID NO: 145 or SEQ ID NO: 146, such as, e.g., a
conservative morbillivirus fusogenic peptide domain variant of SEQ
ID NO: 137, SEQ ID NO: 138, SEQ ID NO: 139, SEQ ID NO: 140, SEQ ID
NO: 141, SEQ ID NO: 142, SEQ ID NO: 143, SEQ ID NO: 144, SEQ ID NO:
145 or SEQ ID NO: 146, a non-conservative morbillivirus fusogenic
peptide domain variant of SEQ ID NO: 137, SEQ ID NO: 138, SEQ ID
NO: 139, SEQ ID NO: 140, SEQ ID NO: 141, SEQ ID NO: 142, SEQ ID NO:
143, SEQ ID NO: 144, SEQ ID NO: 145 or SEQ ID NO: 146, an active
morbillivirus fusogenic peptide domain fragment of SEQ ID NO: 137,
SEQ ID NO: 138, SEQ ID NO: 139, SEQ ID NO: 140, SEQ ID NO: 141, SEQ
ID NO: 142, SEQ ID NO: 143, SEQ ID NO: 144, SEQ ID NO: 145 or SEQ
ID NO: 146, or any combination thereof.
[0199] In other aspects of this embodiment, a morbillivirus
fusogenic peptide domain comprises a polypeptide having, e.g., at
least 70% amino acid identity with SEQ ID NO: 137, SEQ ID NO: 138,
SEQ ID NO: 139, SEQ ID NO: 140, SEQ ID NO: 141, SEQ ID NO: 142, SEQ
ID NO: 143, SEQ ID NO: 144, SEQ ID NO: 145 or SEQ ID NO: 146, at
least 75% amino acid identity with SEQ ID NO: 137, SEQ ID NO: 138,
SEQ ID NO: 139, SEQ ID NO: 140, SEQ ID NO: 141, SEQ ID NO: 142, SEQ
ID NO: 143, SEQ ID NO: 144, SEQ ID NO: 145 or SEQ ID NO: 146, at
least 80% amino acid identity with SEQ ID NO: 137, SEQ ID NO: 138,
SEQ ID NO: 139, SEQ ID NO: 140, SEQ ID NO: 141, SEQ ID NO: 142, SEQ
ID NO: 143, SEQ ID NO: 144, SEQ ID NO: 145 or SEQ ID NO: 146, at
least 85% amino acid identity with SEQ ID NO: 137, SEQ ID NO: 138,
SEQ ID NO: 139, SEQ ID NO: 140, SEQ ID NO: 141, SEQ ID NO: 142, SEQ
ID NO: 143, SEQ ID NO: 144, SEQ ID NO: 145 or SEQ ID NO: 146, at
least 90% amino acid identity with SEQ ID NO: 137, SEQ ID NO: 138,
SEQ ID NO: 139, SEQ ID NO: 140, SEQ ID NO: 141, SEQ ID NO: 142, SEQ
ID NO: 143, SEQ ID NO: 144, SEQ ID NO: 145 or SEQ ID NO: 146 or at
least 95% amino acid identity with SEQ ID NO: 137, SEQ ID NO: 138,
SEQ ID NO: 139, SEQ ID NO: 140, SEQ ID NO: 141, SEQ ID NO: 142, SEQ
ID NO: 143, SEQ ID NO: 144, SEQ ID NO: 145 or SEQ ID NO: 146. In
yet other aspects of this embodiment, a morbillivirus fusogenic
peptide domain comprises a polypeptide having, e.g., at most 70%
amino acid identity with SEQ ID NO: 137, SEQ ID NO: 138, SEQ ID NO:
139, SEQ ID NO: 140, SEQ ID NO: 141, SEQ ID NO: 142, SEQ ID NO:
143, SEQ ID NO: 144, SEQ ID NO: 145 or SEQ ID NO: 146, at most 75%
amino acid identity with SEQ ID NO: 137, SEQ ID NO: 138, SEQ ID NO:
139, SEQ ID NO: 140, SEQ ID NO: 141, SEQ ID NO: 142, SEQ ID NO:
143, SEQ ID NO: 144, SEQ ID NO: 145 or SEQ ID NO: 146, at most 80%
amino acid identity with SEQ ID NO: 137, SEQ ID NO: 138, SEQ ID NO:
139, SEQ ID NO: 140, SEQ ID NO: 141, SEQ ID NO: 142, SEQ ID NO:
143, SEQ ID NO: 144, SEQ ID NO: 145 or SEQ ID NO: 146, at most 85%
amino acid identity with SEQ ID NO: 137, SEQ ID NO: 138, SEQ ID NO:
139, SEQ ID NO: 140, SEQ ID NO: 141, SEQ ID NO: 142, SEQ ID NO:
143, SEQ ID NO: 144, SEQ ID NO: 145 or SEQ ID NO: 146, at most 90%
amino acid identity with SEQ ID NO: 137, SEQ ID NO: 138, SEQ ID NO:
139, SEQ ID NO: 140, SEQ ID NO: 141, SEQ ID NO: 142, SEQ ID NO:
143, SEQ ID NO: 144, SEQ ID NO: 145 or SEQ ID NO: 146 or at most
95% amino acid identity with SEQ ID NO: 137, SEQ ID NO: 138, SEQ ID
NO: 139, SEQ ID NO: 140, SEQ ID NO: 141, SEQ ID NO: 142, SEQ ID NO:
143, SEQ ID NO: 144, SEQ ID NO: 145 or SEQ ID NO: 146.
[0200] In other aspects of this embodiment, a morbillivirus
fusogenic peptide domain comprises a polypeptide having, e.g., at
most one, two, three, four, five, six, seven, eight, nine or 10
non-contiguous amino acid substitutions relative to SEQ ID NO: 137,
SEQ ID NO: 138, SEQ ID NO: 139, SEQ ID NO: 140, SEQ ID NO: 141, SEQ
ID NO: 142, SEQ ID NO: 143, SEQ ID NO: 144, SEQ ID NO: 145 or SEQ
ID NO: 146. In other aspects of this embodiment, a morbillivirus
fusogenic peptide domain comprises a polypeptide having, e.g., at
least one, two, three, four, five, six, seven, eight, nine or 10
non-contiguous amino acid substitutions relative to SEQ ID NO: 137,
SEQ ID NO: 138, SEQ ID NO: 139, SEQ ID NO: 140, SEQ ID NO: 141, SEQ
ID NO: 142, SEQ ID NO: 143, SEQ ID NO: 144, SEQ ID NO: 145 or SEQ
ID NO: 146. In yet other aspects of this embodiment, a
morbillivirus fusogenic peptide domain comprises a polypeptide
having, e.g., at most one, two, three, four, five, six, seven,
eight, nine or 10 non-contiguous amino acid deletions relative to
SEQ ID NO: 137, SEQ ID NO: 138, SEQ ID NO: 139, SEQ ID NO: 140, SEQ
ID NO: 141, SEQ ID NO: 142, SEQ ID NO: 143, SEQ ID NO: 144, SEQ ID
NO: 145 or SEQ ID NO: 146. In other aspects of this embodiment, a
morbillivirus fusogenic peptide domain comprises a polypeptide
having, e.g., at least one, two, three, four, five, six, seven,
eight, nine or 10 non-contiguous amino acid deletions relative to
SEQ ID NO: 137, SEQ ID NO: 138, SEQ ID NO: 139, SEQ ID NO: 140, SEQ
ID NO: 141, SEQ ID NO: 142, SEQ ID NO: 143, SEQ ID NO: 144, SEQ ID
NO: 145 or SEQ ID NO: 146. In still other aspects of this
embodiment, a morbillivirus fusogenic peptide domain comprises a
polypeptide having, e.g., at most one, two, three, four, five, six,
seven, eight, nine or 10 non-contiguous amino acid additions
relative to SEQ ID NO: 137, SEQ ID NO: 138, SEQ ID NO: 139, SEQ ID
NO: 140, SEQ ID NO: 141, SEQ ID NO: 142, SEQ ID NO: 143, SEQ ID NO:
144, SEQ ID NO: 145 or SEQ ID NO: 146. In other aspects of this
embodiment, a morbillivirus fusogenic peptide domain comprises a
polypeptide having, e.g., at least one, two, three, four, five,
six, seven, eight, nine or 10 non-contiguous amino acid additions
relative to SEQ ID NO: 137, SEQ ID NO: 138, SEQ ID NO: 139, SEQ ID
NO: 140, SEQ ID NO: 141, SEQ ID NO: 142, SEQ ID NO: 143, SEQ ID NO:
144, SEQ ID NO: 145 or SEQ ID NO: 146.
[0201] In other aspects of this embodiment, a morbillivirus
fusogenic peptide domain comprises a polypeptide having, e.g., at
most one, two, three, four, five, six, seven, eight, nine or 10
contiguous amino acid substitutions relative to SEQ ID NO: 137, SEQ
ID NO: 138, SEQ ID NO: 139, SEQ ID NO: 140, SEQ ID NO: 141, SEQ ID
NO: 142, SEQ ID NO: 143, SEQ ID NO: 144, SEQ ID NO: 145 or SEQ ID
NO: 146. In other aspects of this embodiment, a morbillivirus
fusogenic peptide domain comprises a polypeptide having, e.g., at
least one, two, three, four, five, six, seven, eight, nine or 10
contiguous amino acid substitutions relative to SEQ ID NO: 137, SEQ
ID NO: 138, SEQ ID NO: 139, SEQ ID NO: 140, SEQ ID NO: 141, SEQ ID
NO: 142, SEQ ID NO: 143, SEQ ID NO: 144, SEQ ID NO: 145 or SEQ ID
NO: 146. In yet other aspects of this embodiment, a morbillivirus
fusogenic peptide domain comprises a polypeptide having, e.g., at
most one, two, three, four, five, six, seven, eight, nine or 10
contiguous amino acid deletions relative to SEQ ID NO: 137, SEQ ID
NO: 138, SEQ ID NO: 139, SEQ ID NO: 140, SEQ ID NO: 141, SEQ ID NO:
142, SEQ ID NO: 143, SEQ ID NO: 144, SEQ ID NO: 145 or SEQ ID NO:
146. In other aspects of this embodiment, a morbillivirus fusogenic
peptide domain comprises a polypeptide having, e.g., at least one,
two, three, four, five, six, seven, eight, nine or 10 contiguous
amino acid deletions relative to SEQ ID NO: 137, SEQ ID NO: 138,
SEQ ID NO: 139, SEQ ID NO: 140, SEQ ID NO: 141, SEQ ID NO: 142, SEQ
ID NO: 143, SEQ ID NO: 144, SEQ ID NO: 145 or SEQ ID NO: 146. In
still other aspects of this embodiment, a morbillivirus fusogenic
peptide domain comprises a polypeptide having, e.g., at most one,
two, three, four, five, six, seven, eight, nine or 10 contiguous
amino acid additions relative to SEQ ID NO: 137, SEQ ID NO: 138,
SEQ ID NO: 139, SEQ ID NO: 140, SEQ ID NO: 141, SEQ ID NO: 142, SEQ
ID NO: 143, SEQ ID NO: 144, SEQ ID NO: 145 or SEQ ID NO: 146. In
other aspects of this embodiment, a morbillivirus fusogenic peptide
domain comprises a polypeptide having, e.g., at least one, two,
three, four, five, six, seven, eight, nine or 10 contiguous amino
acid additions relative to SEQ ID NO: 137, SEQ ID NO: 138, SEQ ID
NO: 139, SEQ ID NO: 140, SEQ ID NO: 141, SEQ ID NO: 142, SEQ ID NO:
143, SEQ ID NO: 144, SEQ ID NO: 145 or SEQ ID NO: 146.
[0202] In another embodiment, a Clostridial toxin translocation
facilitating domain comprises an avulavirus fusogenic peptide
domain. In another aspect of this embodiment, an avulavirus
fusogenic peptide domain comprises a naturally occurring avulavirus
fusogenic peptide domain variant, such as, e.g., an avulavirus
fusogenic peptide domain isoform or an avulavirus fusogenic peptide
domain subtype. In another aspect of this embodiment, an avulavirus
fusogenic peptide domain comprises a naturally occurring avulavirus
fusogenic peptide domain variant of SEQ ID NO: 147, SEQ ID NO: 148,
SEQ ID NO: 149, SEQ ID NO: 150, SEQ ID NO: 151, SEQ ID NO: 152, SEQ
ID NO: 153, SEQ ID NO: 154, SEQ ID NO: 155, SEQ ID NO: 156, SEQ ID
NO: 157, SEQ ID NO: 158 or SEQ ID NO: 159, such as, e.g., an
avulavirus fusogenic peptide domain isoform of SEQ ID NO: 147, SEQ
ID NO: 148, SEQ ID NO: 149, SEQ ID NO: 150, SEQ ID NO: 151, SEQ ID
NO: 152, SEQ ID NO: 153, SEQ ID NO: 154, SEQ ID NO: 155, SEQ ID NO:
156, SEQ ID NO: 157, SEQ ID NO: 158 or SEQ ID NO: 159 or an
avulavirus fusogenic peptide domain subtype of SEQ ID NO: 147, SEQ
ID NO: 148, SEQ ID NO: 149, SEQ ID NO: 150, SEQ ID NO: 151, SEQ ID
NO: 152, SEQ ID NO: 153, SEQ ID NO: 154, SEQ ID NO: 155, SEQ ID NO:
156, SEQ ID NO: 157, SEQ ID NO: 158 or SEQ ID NO: 159. In still
another aspect of this embodiment, an avulavirus fusogenic peptide
domain comprises a non-naturally occurring avulavirus fusogenic
peptide domain variant, such as, e.g., a conservative avulavirus
fusogenic peptide domain variant, a non-conservative avulavirus
fusogenic peptide domain variant, an avulavirus fusogenic peptide
domain chimeric, an active avulavirus fusogenic peptide domain
fragment, or any combination thereof. In still another aspect of
this embodiment, an avulavirus fusogenic peptide domain comprises
amino acids a non-naturally occurring avulavirus fusogenic peptide
domain variant of SEQ ID NO: 147, SEQ ID NO: 148, SEQ ID NO: 149,
SEQ ID NO: 150, SEQ ID NO: 151, SEQ ID NO: 152, SEQ ID NO: 153, SEQ
ID NO: 154, SEQ ID NO: 155, SEQ ID NO: 156, SEQ ID NO: 157, SEQ ID
NO: 158 or SEQ ID NO: 159, such as, e.g., a conservative avulavirus
fusogenic peptide domain variant of SEQ ID NO: 147, SEQ ID NO: 148,
SEQ ID NO: 149, SEQ ID NO: 150, SEQ ID NO: 151, SEQ ID NO: 152, SEQ
ID NO: 153, SEQ ID NO: 154, SEQ ID NO: 155, SEQ ID NO: 156, SEQ ID
NO: 157, SEQ ID NO: 158 or SEQ ID NO: 159, a non-conservative
avulavirus fusogenic peptide domain variant of SEQ ID NO: 147, SEQ
ID NO: 148, SEQ ID NO: 149, SEQ ID NO: 150, SEQ ID NO: 151, SEQ ID
NO: 152, SEQ ID NO: 153, SEQ ID NO: 154, SEQ ID NO: 155, SEQ ID NO:
156, SEQ ID NO: 157, SEQ ID NO: 158 or SEQ ID NO: 159, an active
avulavirus fusogenic peptide domain fragment of SEQ ID NO: 147, SEQ
ID NO: 148, SEQ ID NO: 149, SEQ ID NO: 150, SEQ ID NO: 151, SEQ ID
NO: 152, SEQ ID NO: 153, SEQ ID NO: 154, SEQ ID NO: 155, SEQ ID NO:
156, SEQ ID NO: 157, SEQ ID NO: 158 or SEQ ID NO: 159, or any
combination thereof.
[0203] In other aspects of this embodiment, an avulavirus fusogenic
peptide domain comprises a polypeptide having, e.g., at least 70%
amino acid identity with SEQ ID NO: 147, SEQ ID NO: 148, SEQ ID NO:
149, SEQ ID NO: 150, SEQ ID NO: 151, SEQ ID NO: 152, SEQ ID NO:
153, SEQ ID NO: 154, SEQ ID NO: 155, SEQ ID NO: 156, SEQ ID NO:
157, SEQ ID NO: 158 or SEQ ID NO: 159, at least 75% amino acid
identity with SEQ ID NO: 147, SEQ ID NO: 148, SEQ ID NO: 149, SEQ
ID NO: 150, SEQ ID NO: 151, SEQ ID NO: 152, SEQ ID NO: 153, SEQ ID
NO: 154, SEQ ID NO: 155, SEQ ID NO: 156, SEQ ID NO: 157, SEQ ID NO:
158 or SEQ ID NO: 159, at least 80% amino acid identity with SEQ ID
NO: 147, SEQ ID NO: 148, SEQ ID NO: 149, SEQ ID NO: 150, SEQ ID NO:
151, SEQ ID NO: 152, SEQ ID NO: 153, SEQ ID NO: 154, SEQ ID NO:
155, SEQ ID NO: 156, SEQ ID NO: 157, SEQ ID NO: 158 or SEQ ID NO:
159, at least 85% amino acid identity with SEQ ID NO: 147, SEQ ID
NO: 148, SEQ ID NO: 149, SEQ ID NO: 150, SEQ ID NO: 151, SEQ ID NO:
152, SEQ ID NO: 153, SEQ ID NO: 154, SEQ ID NO: 155, SEQ ID NO:
156, SEQ ID NO: 157, SEQ ID NO: 158 or SEQ ID NO: 159, at least 90%
amino acid identity with SEQ ID NO: 147, SEQ ID NO: 148, SEQ ID NO:
149, SEQ ID NO: 150, SEQ ID NO: 151, SEQ ID NO: 152, SEQ ID NO:
153, SEQ ID NO: 154, SEQ ID NO: 155, SEQ ID NO: 156, SEQ ID NO:
157, SEQ ID NO: 158 or SEQ ID NO: 159 or at least 95% amino acid
identity with SEQ ID NO: 147, SEQ ID NO: 148, SEQ ID NO: 149, SEQ
ID NO: 150, SEQ ID NO: 151, SEQ ID NO: 152, SEQ ID NO: 153, SEQ ID
NO: 154, SEQ ID NO: 155, SEQ ID NO: 156, SEQ ID NO: 157, SEQ ID NO:
158 or SEQ ID NO: 159. In yet other aspects of this embodiment, an
avulavirus fusogenic peptide domain comprises a polypeptide having,
e.g., at most 70% amino acid identity with SEQ ID NO: 147, SEQ ID
NO: 148, SEQ ID NO: 149, SEQ ID NO: 150, SEQ ID NO: 151, SEQ ID NO:
152, SEQ ID NO: 153, SEQ ID NO: 154, SEQ ID NO: 155, SEQ ID NO:
156, SEQ ID NO: 157, SEQ ID NO: 158 or SEQ ID NO: 159, at most 75%
amino acid identity with SEQ ID NO: 147, SEQ ID NO: 148, SEQ ID NO:
149, SEQ ID NO: 150, SEQ ID NO: 151, SEQ ID NO: 152, SEQ ID NO:
153, SEQ ID NO: 154, SEQ ID NO: 155, SEQ ID NO: 156, SEQ ID NO:
157, SEQ ID NO: 158 or SEQ ID NO: 159, at most 80% amino acid
identity with SEQ ID NO: 147, SEQ ID NO: 148, SEQ ID NO: 149, SEQ
ID NO: 150, SEQ ID NO: 151, SEQ ID NO: 152, SEQ ID NO: 153, SEQ ID
NO: 154, SEQ ID NO: 155, SEQ ID NO: 156, SEQ ID NO: 157, SEQ ID NO:
158 or SEQ ID NO: 159, at most 85% amino acid identity with SEQ ID
NO: 147, SEQ ID NO: 148, SEQ ID NO: 149, SEQ ID NO: 150, SEQ ID NO:
151, SEQ ID NO: 152, SEQ ID NO: 153, SEQ ID NO: 154, SEQ ID NO:
155, SEQ ID NO: 156, SEQ ID NO: 157, SEQ ID NO: 158 or SEQ ID NO:
159, at most 90% amino acid identity with SEQ ID NO: 147, SEQ ID
NO: 148, SEQ ID NO: 149, SEQ ID NO: 150, SEQ ID NO: 151, SEQ ID NO:
152, SEQ ID NO: 153, SEQ ID NO: 154, SEQ ID NO: 155, SEQ ID NO:
156, SEQ ID NO: 157, SEQ ID NO: 158 or SEQ ID NO: 159 or at most
95% amino acid identity with SEQ ID NO: 147, SEQ ID NO: 148, SEQ ID
NO: 149, SEQ ID NO: 150, SEQ ID NO: 151, SEQ ID NO: 152, SEQ ID NO:
153, SEQ ID NO: 154, SEQ ID NO: 155, SEQ ID NO: 156, SEQ ID NO:
157, SEQ ID NO: 158 or SEQ ID NO: 159.
[0204] In other aspects of this embodiment, an avulavirus fusogenic
peptide domain comprises a polypeptide having, e.g., at most one,
two, three, four, five, six, seven, eight, nine or 10
non-contiguous amino acid substitutions relative to SEQ ID NO: 147,
SEQ ID NO: 148, SEQ ID NO: 149, SEQ ID NO: 150, SEQ ID NO: 151, SEQ
ID NO: 152, SEQ ID NO: 153, SEQ ID NO: 154, SEQ ID NO: 155, SEQ ID
NO: 156, SEQ ID NO: 157, SEQ ID NO: 158 or SEQ ID NO: 159. In other
aspects of this embodiment, an avulavirus fusogenic peptide domain
comprises a polypeptide having, e.g., at least one, two, three,
four, five, six, seven, eight, nine or 10 non-contiguous amino acid
substitutions relative to SEQ ID NO: 147, SEQ ID NO: 148, SEQ ID
NO: 149, SEQ ID NO: 150, SEQ ID NO: 151, SEQ ID NO: 152, SEQ ID NO:
153, SEQ ID NO: 154, SEQ ID NO: 155, SEQ ID NO: 156, SEQ ID NO:
157, SEQ ID NO: 158 or SEQ ID NO: 159. In yet other aspects of this
embodiment, an avulavirus fusogenic peptide domain comprises a
polypeptide having, e.g., at most one, two, three, four, five, six,
seven, eight, nine or 10 non-contiguous amino acid deletions
relative to SEQ ID NO: 147, SEQ ID NO: 148, SEQ ID NO: 149, SEQ ID
NO: 150, SEQ ID NO: 151, SEQ ID NO: 152, SEQ ID NO: 153, SEQ ID NO:
154, SEQ ID NO: 155, SEQ ID NO: 156, SEQ ID NO: 157, SEQ ID NO: 158
or SEQ ID NO: 159. In other aspects of this embodiment, an
avulavirus fusogenic peptide domain comprises a polypeptide having,
e.g., at least one, two, three, four, five, six, seven, eight, nine
or 10 non-contiguous amino acid deletions relative to SEQ ID NO:
147, SEQ ID NO: 148, SEQ ID NO: 149, SEQ ID NO: 150, SEQ ID NO:
151, SEQ ID NO: 152, SEQ ID NO: 153, SEQ ID NO: 154, SEQ ID NO:
155, SEQ ID NO: 156, SEQ ID NO: 157, SEQ ID NO: 158 or SEQ ID NO:
159. In still other aspects of this embodiment, an avulavirus
fusogenic peptide domain comprises a polypeptide having, e.g., at
most one, two, three, four, five, six, seven, eight, nine or 10
non-contiguous amino acid additions relative to SEQ ID NO: 147, SEQ
ID NO: 148, SEQ ID NO: 149, SEQ ID NO: 150, SEQ ID NO: 151, SEQ ID
NO: 152, SEQ ID NO: 153, SEQ ID NO: 154, SEQ ID NO: 155, SEQ ID NO:
156, SEQ ID NO: 157, SEQ ID NO: 158 or SEQ ID NO: 159. In other
aspects of this embodiment, an avulavirus fusogenic peptide domain
comprises a polypeptide having, e.g., at least one, two, three,
four, five, six, seven, eight, nine or 10 non-contiguous amino acid
additions relative to SEQ ID NO: 147, SEQ ID NO: 148, SEQ ID NO:
149, SEQ ID NO: 150, SEQ ID NO: 151, SEQ ID NO: 152, SEQ ID NO:
153, SEQ ID NO: 154, SEQ ID NO: 155, SEQ ID NO: 156, SEQ ID NO:
157, SEQ ID NO: 158 or SEQ ID NO: 159.
[0205] In other aspects of this embodiment, an avulavirus fusogenic
peptide domain comprises a polypeptide having, e.g., at most one,
two, three, four, five, six, seven, eight, nine or 10 contiguous
amino acid substitutions relative to SEQ ID NO: 147, SEQ ID NO:
148, SEQ ID NO: 149, SEQ ID NO: 150, SEQ ID NO: 151, SEQ ID NO:
152, SEQ ID NO: 153, SEQ ID NO: 154, SEQ ID NO: 155, SEQ ID NO:
156, SEQ ID NO: 157, SEQ ID NO: 158 or SEQ ID NO: 159. In other
aspects of this embodiment, an avulavirus fusogenic peptide domain
comprises a polypeptide having, e.g., at least one, two, three,
four, five, six, seven, eight, nine or 10 contiguous amino acid
substitutions relative to SEQ ID NO: 147, SEQ ID NO: 148, SEQ ID
NO: 149, SEQ ID NO: 150, SEQ ID NO: 151, SEQ ID NO: 152, SEQ ID NO:
153, SEQ ID NO: 154, SEQ ID NO: 155, SEQ ID NO: 156, SEQ ID NO:
157, SEQ ID NO: 158 or SEQ ID NO: 159. In yet other aspects of this
embodiment, an avulavirus fusogenic peptide domain comprises a
polypeptide having, e.g., at most one, two, three, four, five, six,
seven, eight, nine or 10 contiguous amino acid deletions relative
to SEQ ID NO: 147, SEQ ID NO: 148, SEQ ID NO: 149, SEQ ID NO: 150,
SEQ ID NO: 151, SEQ ID NO: 152, SEQ ID NO: 153, SEQ ID NO: 154, SEQ
ID NO: 155, SEQ ID NO: 156, SEQ ID NO: 157, SEQ ID NO: 158 or SEQ
ID NO: 159. In other aspects of this embodiment, an avulavirus
fusogenic peptide domain comprises a polypeptide having, e.g., at
least one, two, three, four, five, six, seven, eight, nine or 10
contiguous amino acid deletions relative to SEQ ID NO: 147, SEQ ID
NO: 148, SEQ ID NO: 149, SEQ ID NO: 150, SEQ ID NO: 151, SEQ ID NO:
152, SEQ ID NO: 153, SEQ ID NO: 154, SEQ ID NO: 155, SEQ ID NO:
156, SEQ ID NO: 157, SEQ ID NO: 158 or SEQ ID NO: 159. In still
other aspects of this embodiment, an avulavirus fusogenic peptide
domain comprises a polypeptide having, e.g., at most one, two,
three, four, five, six, seven, eight, nine or 10 contiguous amino
acid additions relative to SEQ ID NO: 147, SEQ ID NO: 148, SEQ ID
NO: 149, SEQ ID NO: 150, SEQ ID NO: 151, SEQ ID NO: 152, SEQ ID NO:
153, SEQ ID NO: 154, SEQ ID NO: 155, SEQ ID NO: 156, SEQ ID NO:
157, SEQ ID NO: 158 or SEQ ID NO: 159. In other aspects of this
embodiment, an avulavirus fusogenic peptide domain comprises a
polypeptide having, e.g., at least one, two, three, four, five,
six, seven, eight, nine or 10 contiguous amino acid additions
relative to SEQ ID NO: 147, SEQ ID NO: 148, SEQ ID NO: 149, SEQ ID
NO: 150, SEQ ID NO: 151, SEQ ID NO: 152, SEQ ID NO: 153, SEQ ID NO:
154, SEQ ID NO: 155, SEQ ID NO: 156, SEQ ID NO: 157, SEQ ID NO: 158
or SEQ ID NO: 159.
[0206] In another embodiment, a Clostridial toxin translocation
facilitating domain comprises a henipavirus fusogenic peptide
domain. In another aspect of this embodiment, a henipavirus
fusogenic peptide domain comprises a naturally occurring
henipavirus fusogenic peptide domain variant, such as, e.g., a
henipavirus fusogenic peptide domain isoform or a henipavirus
fusogenic peptide domain subtype.
[0207] In another aspect of this embodiment, a henipavirus
fusogenic peptide domain comprises a naturally occurring
henipavirus fusogenic peptide domain variant of SEQ ID NO: 160 or
SEQ ID NO: 161, such as, e.g., a henipavirus fusogenic peptide
domain isoform of SEQ ID NO: 160 or SEQ ID NO: 161 or a henipavirus
fusogenic peptide domain subtype of SEQ ID NO: 160 or SEQ ID NO:
161. In still another aspect of this embodiment, a henipavirus
fusogenic peptide domain comprises a non-naturally occurring
henipavirus fusogenic peptide domain variant, such as, e.g., a
conservative henipavirus fusogenic peptide domain variant, a
non-conservative henipavirus fusogenic peptide domain variant, a
henipavirus fusogenic peptide domain chimeric, an active
henipavirus fusogenic peptide domain fragment, or any combination
thereof. In still another aspect of this embodiment, a henipavirus
fusogenic peptide domain comprises amino acids a non-naturally
occurring henipavirus fusogenic peptide domain variant of SEQ ID
NO: 160 or SEQ ID NO: 161, such as, e.g., a conservative
henipavirus fusogenic peptide domain variant of SEQ ID NO: 160 or
SEQ ID NO: 161, a non-conservative henipavirus fusogenic peptide
domain variant of SEQ ID NO: 160 or SEQ ID NO: 161, an active
henipavirus fusogenic peptide domain fragment of SEQ ID NO: 160 or
SEQ ID NO: 161, or any combination thereof.
[0208] In other aspects of this embodiment, a henipavirus fusogenic
peptide domain comprises a polypeptide having, e.g., at least 70%
amino acid identity with SEQ ID NO: 160 or SEQ ID NO: 161, at least
75% amino acid identity with SEQ ID NO: 160 or SEQ ID NO: 161, at
least 80% amino acid identity with SEQ ID NO: 160 or SEQ ID NO:
161, at least 85% amino acid identity with SEQ ID NO: 160 or SEQ ID
NO: 161, at least 90% amino acid identity with SEQ ID NO: 160 or
SEQ ID NO: 161 or at least 95% amino acid identity with SEQ ID NO:
160 or SEQ ID NO: 161. In yet other aspects of this embodiment, a
henipavirus fusogenic peptide domain comprises a polypeptide
having, e.g., at most 70% amino acid identity with SEQ ID NO: 160
or SEQ ID NO: 161, at most 75% amino acid identity with SEQ ID NO:
160 or SEQ ID NO: 161, at most 80% amino acid identity with SEQ ID
NO: 160 or SEQ ID NO: 161, at most 85% amino acid identity with SEQ
ID NO: 160 or SEQ ID NO: 161, at most 90% amino acid identity with
SEQ ID NO: 160 or SEQ ID NO: 161 or at most 95% amino acid identity
with SEQ ID NO: 160 or SEQ ID NO: 161.
[0209] In other aspects of this embodiment, a henipavirus fusogenic
peptide domain comprises a polypeptide having, e.g., at most one,
two, three, four, five, six, seven, eight, nine or 10
non-contiguous amino acid substitutions relative to SEQ ID NO: 160
or SEQ ID NO: 161. In other aspects of this embodiment, a
henipavirus fusogenic peptide domain comprises a polypeptide
having, e.g., at least one, two, three, four, five, six, seven,
eight, nine or 10 non-contiguous amino acid substitutions relative
to SEQ ID NO: 160 or SEQ ID NO: 161. In yet other aspects of this
embodiment, a henipavirus fusogenic peptide domain comprises a
polypeptide having, e.g., at most one, two, three, four, five, six,
seven, eight, nine or 10 non-contiguous amino acid deletions
relative to SEQ ID NO: 160 or SEQ ID NO: 161. In other aspects of
this embodiment, a henipavirus fusogenic peptide domain comprises a
polypeptide having, e.g., at least one, two, three, four, five,
six, seven, eight, nine or 10 non-contiguous amino acid deletions
relative to SEQ ID NO: 160 or SEQ ID NO: 161. In still other
aspects of this embodiment, a henipavirus fusogenic peptide domain
comprises a polypeptide having, e.g., at most one, two, three,
four, five, six, seven, eight, nine or 10 non-contiguous amino acid
additions relative to SEQ ID NO: 160 or SEQ ID NO: 161. In other
aspects of this embodiment, a henipavirus fusogenic peptide domain
comprises a polypeptide having, e.g., at least one, two, three,
four, five, six, seven, eight, nine or 10 non-contiguous amino acid
additions relative to SEQ ID NO: 160 or SEQ ID NO: 161.
[0210] In other aspects of this embodiment, a henipavirus fusogenic
peptide domain comprises a polypeptide having, e.g., at most one,
two, three, four, five, six, seven, eight, nine or 10 contiguous
amino acid substitutions relative to SEQ ID NO: 160 or SEQ ID NO:
161. In other aspects of this embodiment, a henipavirus fusogenic
peptide domain comprises a polypeptide having, e.g., at least one,
two, three, four, five, six, seven, eight, nine or 10 contiguous
amino acid substitutions relative to SEQ ID NO: 160 or SEQ ID NO:
161. In yet other aspects of this embodiment, a henipavirus
fusogenic peptide domain comprises a polypeptide having, e.g., at
most one, two, three, four, five, six, seven, eight, nine or 10
contiguous amino acid deletions relative to SEQ ID NO: 160 or SEQ
ID NO: 161. In other aspects of this embodiment, a henipavirus
fusogenic peptide domain comprises a polypeptide having, e.g., at
least one, two, three, four, five, six, seven, eight, nine or 10
contiguous amino acid deletions relative to SEQ ID NO: 160 or SEQ
ID NO: 161. In still other aspects of this embodiment, a
henipavirus fusogenic peptide domain comprises a polypeptide
having, e.g., at most one, two, three, four, five, six, seven,
eight, nine or 10 contiguous amino acid additions relative to SEQ
ID NO: 160 or SEQ ID NO: 161. In other aspects of this embodiment,
a henipavirus fusogenic peptide domain comprises a polypeptide
having, e.g., at least one, two, three, four, five, six, seven,
eight, nine or 10 contiguous amino acid additions relative to SEQ
ID NO: 160 or SEQ ID NO: 161.
[0211] In another embodiment, a Clostridial toxin translocation
facilitating domain comprises a metapneumovirus fusogenic peptide
domain. In another aspect of this embodiment, a metapneumovirus
fusogenic peptide domain comprises a naturally occurring
metapneumovirus fusogenic peptide domain variant, such as, e.g., a
metapneumovirus fusogenic peptide domain isoform or a
metapneumovirus fusogenic peptide domain subtype. In another aspect
of this embodiment, a metapneumovirus fusogenic peptide domain
comprises a naturally occurring metapneumovirus fusogenic peptide
domain variant of SEQ ID NO: 162, such as, e.g., a metapneumovirus
fusogenic peptide domain isoform of SEQ ID NO: 162 or a
metapneumovirus fusogenic peptide domain subtype of SEQ ID NO: 162.
In still another aspect of this embodiment, a metapneumovirus
fusogenic peptide domain comprises a non-naturally occurring
metapneumovirus fusogenic peptide domain variant, such as, e.g., a
conservative metapneumovirus fusogenic peptide domain variant, a
non-conservative metapneumovirus fusogenic peptide domain variant,
a metapneumovirus fusogenic peptide domain chimeric, an active
metapneumovirus fusogenic peptide domain fragment, or any
combination thereof. In still another aspect of this embodiment, a
metapneumovirus fusogenic peptide domain comprises amino acids a
non-naturally occurring metapneumovirus fusogenic peptide domain
variant of SEQ ID NO: 162, such as, e.g., a conservative
metapneumovirus fusogenic peptide domain variant of SEQ ID NO: 162,
a non-conservative metapneumovirus fusogenic peptide domain variant
of SEQ ID NO: 162, an active metapneumovirus fusogenic peptide
domain fragment of SEQ ID NO: 162, or any combination thereof.
[0212] In other aspects of this embodiment, a metapneumovirus
fusogenic peptide domain comprises a polypeptide having, e.g., at
least 70% amino acid identity with SEQ ID NO: 162, at least 75%
amino acid identity with SEQ ID NO: 162, at least 80% amino acid
identity with SEQ ID NO: 162, at least 85% amino acid identity with
SEQ ID NO: 162, at least 90% amino acid identity with SEQ ID NO:
162 or at least 95% amino acid identity with SEQ ID NO: 162. In yet
other aspects of this embodiment, a metapneumovirus fusogenic
peptide domain comprises a polypeptide having, e.g., at most 70%
amino acid identity with SEQ ID NO: 162, at most 75% amino acid
identity with SEQ ID NO: 162, at most 80% amino acid identity with
SEQ ID NO: 162, at most 85% amino acid identity with SEQ ID NO:
162, at most 90% amino acid identity with SEQ ID NO: 162 or at most
95% amino acid identity with SEQ ID NO: 162.
[0213] In other aspects of this embodiment, a metapneumovirus
fusogenic peptide domain comprises a polypeptide having, e.g., at
most one, two, three, four, five, six, seven, eight, nine or 10
non-contiguous amino acid substitutions relative to SEQ ID NO: 162.
In other aspects of this embodiment, a metapneumovirus fusogenic
peptide domain comprises a polypeptide having, e.g., at least one,
two, three, four, five, six, seven, eight, nine or 10
non-contiguous amino acid substitutions relative to SEQ ID NO: 162.
In yet other aspects of this embodiment, a metapneumovirus
fusogenic peptide domain comprises a polypeptide having, e.g., at
most one, two, three, four, five, six, seven, eight, nine or 10
non-contiguous amino acid deletions relative to SEQ ID NO: 162. In
other aspects of this embodiment, a metapneumovirus fusogenic
peptide domain comprises a polypeptide having, e.g., at least one,
two, three, four, five, six, seven, eight, nine or 10
non-contiguous amino acid deletions relative to SEQ ID NO: 162. In
still other aspects of this embodiment, a metapneumovirus fusogenic
peptide domain comprises a polypeptide having, e.g., at most one,
two, three, four, five, six, seven, eight, nine or 10
non-contiguous amino acid additions relative to SEQ ID NO: 162. In
other aspects of this embodiment, a metapneumovirus fusogenic
peptide domain comprises a polypeptide having, e.g., at least one,
two, three, four, five, six, seven, eight, nine or 10
non-contiguous amino acid additions relative to SEQ ID NO: 162.
[0214] In other aspects of this embodiment, a metapneumovirus
fusogenic peptide domain comprises a polypeptide having, e.g., at
most one, two, three, four, five, six, seven, eight, nine or 10
contiguous amino acid substitutions relative to SEQ ID NO: 162. In
other aspects of this embodiment, a metapneumovirus fusogenic
peptide domain comprises a polypeptide having, e.g., at least one,
two, three, four, five, six, seven, eight, nine or 10 contiguous
amino acid substitutions relative to SEQ ID NO: 162. In yet other
aspects of this embodiment, a metapneumovirus fusogenic peptide
domain comprises a polypeptide having, e.g., at most one, two,
three, four, five, six, seven, eight, nine or 10 contiguous amino
acid deletions relative to SEQ ID NO: 162. In other aspects of this
embodiment, a metapneumovirus fusogenic peptide domain comprises a
polypeptide having, e.g., at least one, two, three, four, five,
six, seven, eight, nine or 10 contiguous amino acid deletions
relative to SEQ ID NO: 162. In still other aspects of this
embodiment, a metapneumovirus fusogenic peptide domain comprises a
polypeptide having, e.g., at most one, two, three, four, five, six,
seven, eight, nine or 10 contiguous amino acid additions relative
to SEQ ID NO: 162. In other aspects of this embodiment, a
metapneumovirus fusogenic peptide domain comprises a polypeptide
having, e.g., at least one, two, three, four, five, six, seven,
eight, nine or 10 contiguous amino acid additions relative to SEQ
ID NO: 162.
[0215] Aspects of the present invention provide, in part, an
altered targeting domain. As used herein, the term "altered
targeting domain" means any polypeptide that can selectively bind
to a non-Clostridial toxin receptor present on a non-Clostridial
toxin target cell and initiate the overall internalization
mechanism whereby the modified Clostridial toxin disclosed in the
present specification intoxicates a target cell. As used herein,
the term "selectively" means having a highly preferred activity or
effect. As used herein, the term "selectively bind" means a
molecule is able to bind its target receptor under physiological
conditions, or in vitro conditions substantially approximating
physiological conditions, to a statistically significantly greater
degree relative to other, non-target receptors. Thus, with
reference to an altered targeting domain of the present
specification, there is a discriminatory binding of the altered
targeting domain to a non-Clostridial toxin receptor presence in a
non-Clostridial toxin target cell.
[0216] Aspects of the present invention provide, in part, an
enhanced targeting domain. As used herein, the term "enhanced
targeting domain" means any polypeptide that can selectively bind
to an endogenous Clostridial toxin receptor found on a Clostridial
toxin target cell and initiate the overall internalization
mechanism whereby the modified Clostridial toxin disclosed in the
present specification intoxicates a target cell with the proviso
that an enhanced targeting domain is not a naturally-occurring
binding domain from a naturally occurring Clostridial toxin. As
used herein, the term "selectively" means having a highly preferred
activity or effect. As used herein, the term "selectively bind"
means a molecule is able to bind its target receptor under
physiological conditions, or in vitro conditions substantially
approximating physiological conditions, to a statistically
significantly greater degree relative to other non-target
receptors. Thus, with reference to an enhanced targeting domain of
the present specification, there is a discriminatory binding of the
enhanced targeting domain to an endogenous Clostridial toxin
receptor.
[0217] An enhanced targeting domain disclosed in the present
specification facilitates the binding activity of the modified
Clostridial toxins disclosed in the present specification to an
endogenous Clostridial toxin receptor located at the surface of a
Clostridial toxin target cell. As used herein, the term "binding
activity" means that one molecule is directly or indirectly
contacting another molecule via at least one intermolecular or
intramolecular force, including, without limitation, a covalent
bond, an ionic bond, a metallic bond, a hydrogen bond, a
hydrophobic interaction, a van der Waals interaction, and the like,
or any combination thereof. "Bound" and "bind" are considered terms
for binding.
[0218] As used herein, the term "binding affinity" means how strong
a molecule's binding activity is for a particular receptor. In
general, high binding affinity results from greater intermolecular
force between a binding domain and its receptor while low binding
affinity involves less intermolecular force between the ligand and
its receptor. High binding affinity involves a longer residence
time for the binding domain at its receptor binding site than is
the case for low binding affinity. As such, a molecule with a high
binding affinity means a lower concentration of that molecule is
required to maximally occupy the binding sites of a receptor and
trigger a physiological response. Conversely, low binding affinity
means a relatively high concentration of a molecule is required
before the receptor binding sites of a receptor is maximally
occupied and the maximum physiological response is achieved. Thus,
modified Clostridial toxins with increased binding activity due to
high binding affinity will allow administration of reduced doses of
the toxin, thereby reducing or preventing unwanted side-effects
associated with toxin dispersal into non-targeted areas.
[0219] As used herein, the term "binding specificity" means how
specific a molecule's binding activity is one particular receptor.
In general, high binding specificity results in a more exclusive
interaction with one particular receptor or subgroup of receptors
while low binding specificity results in a more promiscuous
interaction with a larger group of receptors. As such, a molecule
with a high binding specificity means that molecule will occupy the
binding sites of a particular receptor and trigger a physiological
response. Conversely, low binding specificity means a molecule will
occupy the binding sites of many receptors and trigger a multitude
of physiological responses. Thus, modified Clostridial toxins with
increased binding activity due to high binding specificity will
only target a subgroup of Clostridial toxin target cells, thereby
reducing the side effects associated with the targeting of all
Clostridial toxin target cells.
[0220] It is envisioned that any and all enhanced targeting domains
can be used to practice aspects of the present invention,
including, without limitation, an enhanced targeting domain that
increases binding affinity for an endogenous Clostridial toxin
receptor present on a naturally-occurring Clostridial toxin target
cell; and an enhanced targeting domain that increases binding
specificity for a subgroup of endogenous Clostridial toxin
receptors present on a naturally-occurring Clostridial toxin target
cell.
[0221] Assays that determine modified Clostridial toxin binding
activity or uptake properties can be used to assess whether a
modified Clostridial toxin disclosed in the present specification
has an enhanced binding activity, such as an increased binding
affinity or an increased binding specificity. It is envisioned that
heterogeneous assay types, homogeneous assay types and
non-separating homogeneous assay types can be used to determine the
binding activity of a modified Clostridial toxin with an enhanced
targeting domain, see, e.g., Lutea A. A. de Jong et al.,
Receptor-Binding Assays: Technologies and Applications, 829 J.
Chromatogr. B 1-25 (2005). It is further envisioned that the
binding activity of a modified Clostridial toxin with an enhanced
targeting domain can be determined by affinity chromatography using
immobilized receptors and interfacial optical assays, such as,
e.g., total internal reflection fluorescence (TIRF) and surface
plasmon resonance (SPR). Non-limiting examples of these assays
include, e.g., FCS using diffusion mediated intensity fluctuations,
SPR using a mass-dependent refractive index, TIRF using a
mass-independent refractive index, microarrays using optical
intensity changes and QAC using retention volume.
[0222] As a non-limiting example, cross-linking assays using
radio-labeled or non-radio-labeled modified Clostridial toxins can
determine whether such a modified toxin has an enhanced targeting
activity to a Clostridial toxin receptor as compared to the
Clostridial toxin from which the modified toxin was derived. Other
non-limiting assays include immunocytochemical assays that detect
modified toxin binding using labeled or unlabeled antibodies and
immunoprecipitation assays that detect bound modified toxin using
labeled or unlabeled antibodies. Antibodies useful for these assays
include, without limitation, antibodies selected against a portion
of a Clostridial toxin heavy chain comprising the H.sub.N
translocation domain or a portion of a Clostridial toxin light
chain; or antibodies selected against the Clostridial toxin
receptor. If the antibody is labeled, the binding of the molecule
can be detected by various means, including Western blotting,
direct microscopic observation of the cellular location of the
antibody, measurement of cell or substrate-bound antibody following
a wash step, or electrophoresis, employing techniques well-known to
those of skill in the art. If the antibody is unlabeled, one may
employ a labeled secondary antibody for indirect detection of the
bound molecule, and detection can proceed as for a labeled
antibody. It is understood that these and similar assays that
determine modified Clostridial toxin uptake properties or
characteristics can be useful in determining whether a modified
toxin disclosed in the present specification has an enhanced
targeting activity.
[0223] Assays that monitor the release of a molecule after exposure
to a modified Clostridial toxin disclosed in the present
specification can also be used to assess whether such a modified
toxin has enhanced binding activity to a Clostridial toxin receptor
as compared to the Clostridial toxin from which the modified toxin
was derived. In these assays, a greater or faster inhibition of the
molecule's release would occur in cells exposed to a modified
Clostridial toxin with an enhanced targeting activity after
exposure to a modified Clostridial toxin. Well known assays include
methods that measure inhibition of radio-labeled catecholamine
release from neurons, such as, e.g., [.sup.3H] noradrenaline or
[.sup.3H] dopamine release, see e.g., A Fassio et al., Evidence for
calcium-dependent vesicular transmitter release insensitive to
tetanus toxin and botulinum toxin type F, 90.beta.) Neuroscience
893-902 (1999); and Sara Stigliani et al., The sensitivity of
catecholamine release to botulinum toxin C1 and E suggests
selective targeting of vesicles set into the readily releasable
pool, 85(2) J. Neurochem. 409-421 (2003), or measures catecholamine
release using a fluorometric procedure, see, e.g., Anton de Paiva
et al., A role for the interchain disulfide or its participating
thiols in the internalization of botulinum neurotoxin A revealed by
a toxin derivative that binds to ecto-acceptors and inhibits
transmitter release intracellularly, 268(28) J. Biol. Chem.
20838-20844 (1993); Gary W. Lawrence et al., Distinct exocytotic
responses of intact and permeabilised chromaffin cells after
cleavage of the 25-kDa synaptosomal-associated protein (SNAP-25) or
synaptobrevin by botulinum toxin A or B, 236.beta.) Eur. J.
Biochem. 877-886 (1996); and Patrick Foran et al., Botulinum
neurotoxin C1 cleaves both syntaxin and SNAP-25 in intact and
permeabilized chromaffin cells: correlation with its blockade of
catecholamine release, 35(8) Biochemistry 2630-2636 (1996). Other
non-limiting examples include assays that measure inhibition of
hormone release from endocrine cells, such as, e.g., anterior
pituitary cells or ovarian cells. It is understood that these and
similar assays for molecule release can be useful in determining
whether a modified toxin disclosed in the present specification has
an enhanced targeting activity.
[0224] Assays that detect the cleavage of a Clostridial toxin
substrate after exposure to a modified Clostridial toxin disclosed
in the present specification can also be used to assess whether
such a modified toxin has enhanced binding activity to a
Clostridial toxin receptor as compared to the Clostridial toxin
from which the modified toxin was derived. In these assays,
generation of a Clostridial toxin substrate cleavage-product would
be detected in cells expressing a receptor of interest after
modified Clostridial toxin treatment. Non-limiting examples of
specific Western blotting procedures, as well as well-characterized
reagents, conditions and protocols are readily available from
commercial vendors that include, without limitation, Amersham
Biosciences, Piscataway, N.J.; Bio-Rad Laboratories, Hercules,
Calif.; Pierce Biotechnology, Inc., Rockford, Ill.; Promega
Corporation, Madison, Wis., and Stratagene, Inc., La Jolla, Calif.
It is understood that these and similar assays for Clostridial
toxin substrate cleavage can be useful in determining whether a
modified toxin disclosed in the present specification has an
enhanced targeting activity.
[0225] As non-limiting examples, western blot analysis using an
antibody that specifically recognizes a BoNT/A substrate cleavage
product at the SNAP-25 site can be used to assay whether a modified
toxin has an enhanced binding activity to a Clostridial toxin
receptor as compared to the Clostridial toxin from which the
modified toxin was derived; western blot analysis using an antibody
that specifically recognizes a BoNT/C1 substrate cleavage product
at the SNAP-25 site can be used to assay whether a modified toxin
has an enhanced binding activity to a Clostridial toxin receptor as
compared to the Clostridial toxin from which the modified toxin was
derived; and western blot analysis using an antibody that
specifically recognizes a BoNT/E substrate cleaved product can be
used to assay whether a modified toxin has an enhanced binding
activity to a Clostridial toxin receptor as compared to the
Clostridial toxin from which the modified toxin was derived.
Examples of anti-SNAP-25 antibodies useful for these assays
include, without limitation, rabbit polyclonal anti-SNAP25.sub.197
antiserum pAb anti-SNAP25197 #1 (Allergan, Inc., Irvine, Calif.),
mouse monoclonal anti-SNAP-25 antibody SMI-81 (Sternberger
Monoclonals, Lutherville, Md.), mouse monoclonal anti-SNAP-25
antibody Cl 71.1 (Synaptic Systems, Goettingen, Germany), mouse
monoclonal anti-SNAP-25 antibody Cl 71.2 (Synaptic Systems,
Goettingen, Germany), mouse monoclonal anti-SNAP-25 antibody SP12
(Abcam, Cambridge, Mass.), rabbit polyclonal anti-SNAP-25 antiserum
(Synaptic Systems, Goettingen, Germany), and rabbit polyclonal
anti-SNAP-25 antiserum (Abcam, Cambridge, Mass.).
[0226] As additional non-limiting examples, western blot analysis
using an antibody that specifically recognizes a BoNT/B substrate
cleaved product can be used to assay whether a modified toxin has
an enhanced binding activity to a Clostridial toxin receptor as
compared to the Clostridial toxin from which the modified toxin was
derived; western blot analysis using an antibody that specifically
recognizes a BoNT/D substrate cleaved product can be used to assay
whether a modified toxin has an enhanced binding activity to a
Clostridial toxin receptor as compared to the Clostridial toxin
from which the modified toxin was derived; western blot analysis
using an antibody that specifically recognizes a BoNT/F substrate
cleaved product can be used to assay whether a modified toxin has
an enhanced binding activity to a Clostridial toxin receptor as
compared to the Clostridial toxin from which the modified toxin was
derived; western blot analysis using an antibody that specifically
recognizes a BoNT/G substrate cleaved product can be used to assay
whether a modified toxin has an enhanced binding activity to a
Clostridial toxin receptor as compared to the Clostridial toxin
from which the modified toxin was derived; and western blot
analysis using an antibody that specifically recognizes a TeNT
substrate cleaved product can be used to assay whether a modified
toxin has an enhanced binding activity to a Clostridial toxin
receptor as compared to the Clostridial toxin from which the
modified toxin was derived. Examples of anti-VAMP antibodies useful
for these assays include, without limitation, mouse monoclonal
anti-VAMP-1 antibody Cl 10.1 (Synaptic Systems, Goettingen,
Germany), mouse monoclonal anti-VAMP-1 antibody SP10 (Abcam,
Cambridge, Mass.), mouse monoclonal anti-VAMP-1 antibody SP11
(Abcam, Cambridge, Mass.), rabbit polyclonal anti-VAMP-1 antiserum
(Synaptic Systems, Goettingen, Germany), rabbit polyclonal
anti-VAMP-1 antiserum (Abcam, Cambridge, Mass.), mouse monoclonal
anti-VAMP-2 antibody Cl 69.1 (Synaptic Systems, Goettingen,
Germany), rabbit polyclonal anti-VAMP-2 antiserum (Synaptic
Systems, Goettingen, Germany), rabbit polyclonal anti-VAMP-2
antiserum (Abcam, Cambridge, Mass.), mouse monoclonal anti-VAMP-3
antibody Cl 10.1 (Synaptic Systems, Goettingen, Germany), rabbit
polyclonal anti-VAMP-3 antiserum (Synaptic Systems, Goettingen,
Germany) and rabbit polyclonal anti-VAMP-3 antiserum (Abcam,
Cambridge, Mass.),
[0227] As another non-limiting example, western blot analysis using
an antibody that specifically recognizes a BoNT/C1 substrate
cleaved product at the Syntaxin site can be used to assay whether a
modified toxin has an enhanced binding activity to a Clostridial
toxin receptor as compared to the Clostridial toxin from which the
modified toxin was derived. Examples of anti-Syntaxin antibodies
useful for these assays include, without limitation, mouse
monoclonal anti-Syntaxin-1 antibody Cl 78.2 (Synaptic Systems,
Goettingen, Germany), mouse monoclonal anti-Syntaxin-1A antibody Cl
78.3 (Synaptic Systems, Goettingen, Germany), rabbit polyclonal
anti-Syntaxin-1A antiserum (Synaptic Systems, Goettingen, Germany),
rabbit polyclonal anti-Syntaxin-1B antiserum (Synaptic Systems,
Goettingen, Germany), rabbit polyclonal anti-Syntaxin antiserum
(Abcam, Cambridge, Mass.), rabbit polyclonal anti-Syntaxin-2
antiserum (Abcam, Cambridge, Mass.) and rabbit polyclonal
anti-Syntaxin-3 antiserum (Abcam, Cambridge, Mass.),
[0228] Thus, in an embodiment, a modified Clostridial toxin with an
enhanced targeting activity is a modified Clostridial toxin with an
increased binding affinity for a Clostridial toxin receptor.
Increased binding affinity for a Clostridial toxin receptor is
determined by comparing the binding affinity of the modified
Clostridial toxin to that of the binding affinity for the same
Clostridial toxin receptor of a Clostridial toxin lacking the
enhanced targeting domain modification from which the modified
Clostridial toxin is derived. In aspects of this embodiment, a
modified Clostridial toxin with an increased binding affinity for a
Clostridial toxin receptor exhibits a binding affinity that is,
e.g., at least 10% greater than the binding affinity of the
naturally-occurring Clostridial toxin from which the modified
Clostridial toxin is derived, at least 20% greater than the binding
affinity of the naturally-occurring Clostridial toxin from which
the modified Clostridial toxin is derived, at least 30% greater
than the binding affinity of the naturally-occurring Clostridial
toxin from which the modified Clostridial toxin is derived, at
least 40% greater than the binding affinity of the
naturally-occurring Clostridial toxin from which the modified
Clostridial toxin is derived, at least 50% greater than the binding
affinity of the naturally-occurring Clostridial toxin from which
the modified Clostridial toxin is derived, at least 60% greater
than the binding affinity of the naturally-occurring Clostridial
toxin from which the modified Clostridial toxin is derived, at
least 70% greater than the binding affinity of the
naturally-occurring Clostridial toxin from which the modified
Clostridial toxin is derived, at least 80% greater than the binding
affinity of the naturally-occurring Clostridial toxin from which
the modified Clostridial toxin is derived, at least 90% greater
than the binding affinity of the naturally-occurring Clostridial
toxin from which the modified Clostridial toxin is derived or at
least 100% greater than the binding affinity of the
naturally-occurring Clostridial toxin from which the modified
Clostridial toxin is derived. In aspects of this embodiment, a
modified Clostridial toxin with an increased binding affinity for a
Clostridial toxin receptor exhibits a binding affinity that is,
e.g., at most 10% greater than the binding affinity of the
naturally-occurring Clostridial toxin from which the modified
Clostridial toxin is derived, at most 20% greater than the binding
affinity of the naturally-occurring Clostridial toxin from which
the modified Clostridial toxin is derived, at most 30% greater than
the binding affinity of the naturally-occurring Clostridial toxin
from which the modified Clostridial toxin is derived, at most 40%
greater than the binding affinity of the naturally-occurring
Clostridial toxin from which the modified Clostridial toxin is
derived, at most 50% greater than the binding affinity of the
naturally-occurring Clostridial toxin from which the modified
Clostridial toxin is derived, at most 60% greater than the binding
affinity of the naturally-occurring Clostridial toxin from which
the modified Clostridial toxin is derived, at most 70% greater than
the binding affinity of the naturally-occurring Clostridial toxin
from which the modified Clostridial toxin is derived, at most 80%
greater than the binding affinity of the naturally-occurring
Clostridial toxin from which the modified Clostridial toxin is
derived, at most 90% greater than the binding affinity of the
naturally-occurring Clostridial toxin from which the modified
Clostridial toxin is derived or at most 100% greater than the
binding affinity of the naturally-occurring Clostridial toxin from
which the modified Clostridial toxin is derived.
[0229] In yet other aspects of this embodiment, a modified
Clostridial toxin with an increased binding affinity for a
Clostridial toxin receptor exhibits a binding affinity that is,
e.g., at least two-fold greater than the binding affinity of the
naturally-occurring Clostridial toxin from which the modified
Clostridial toxin is derived, at least three-fold greater than the
binding affinity of the naturally-occurring Clostridial toxin from
which the modified Clostridial toxin is derived, at least four-fold
greater than the binding affinity of the naturally-occurring
Clostridial toxin from which the modified Clostridial toxin is
derived, at least five-fold greater than the binding affinity of
the naturally-occurring Clostridial toxin from which the modified
Clostridial toxin is derived, at least ten-fold greater than the
binding affinity of the naturally-occurring Clostridial toxin from
which the modified Clostridial toxin is derived or at least
twent.gamma.-fold greater than the binding affinity of the
naturally-occurring Clostridial toxin from which the modified
Clostridial toxin is derived. In yet other aspects of this
embodiment, a modified Clostridial toxin with an increased binding
affinity for a Clostridial toxin receptor exhibits a binding
affinity that is, e.g., at most two-fold greater than the binding
affinity of the naturally-occurring Clostridial toxin from which
the modified Clostridial toxin is derived, at most three-fold
greater than the binding affinity of the naturally-occurring
Clostridial toxin from which the modified Clostridial toxin is
derived, at most four-fold greater than the binding affinity of the
naturally-occurring Clostridial toxin from which the modified
Clostridial toxin is derived, at most five-fold greater than the
binding affinity of the naturally-occurring Clostridial toxin from
which the modified Clostridial toxin is derived, at most ten-fold
greater than the binding affinity of the naturally-occurring
Clostridial toxin from which the modified Clostridial toxin is
derived or at most twent.gamma.-fold greater than the binding
affinity of the naturally-occurring Clostridial toxin from which
the modified Clostridial toxin is derived.
[0230] In another embodiment, a modified Clostridial toxin with an
enhanced targeting activity is a modified Clostridial toxin with an
increased binding specificity for a Clostridial toxin receptor.
Increased binding specificity for a Clostridial toxin receptor is
determined by comparing the binding specificity of the modified
Clostridial toxin to that of the binding specificity for the same
Clostridial toxin receptor of a Clostridial toxin lacking the
enhanced targeting domain modification from which the modified
Clostridial toxin is derived. In aspects of this embodiment, a
modified Clostridial toxin with an increased binding specificity
for a Clostridial toxin receptor exhibits a binding specificity
that is, e.g., at least 10% greater than the binding specificity of
the naturally-occurring Clostridial toxin from which the modified
Clostridial toxin is derived, at least 20% greater than the binding
specificity of the naturally-occurring Clostridial toxin from which
the modified Clostridial toxin is derived, at least 30% greater
than the binding specificity of the naturally-occurring Clostridial
toxin from which the modified Clostridial toxin is derived, at
least 40% greater than the binding specificity of the
naturally-occurring Clostridial toxin from which the modified
Clostridial toxin is derived, at least 50% greater than the binding
specificity of the naturally-occurring Clostridial toxin from which
the modified Clostridial toxin is derived, at least 60% greater
than the binding specificity of the naturally-occurring Clostridial
toxin from which the modified Clostridial toxin is derived, at
least 70% greater than the binding specificity of the
naturally-occurring Clostridial toxin from which the modified
Clostridial toxin is derived, at least 80% greater than the binding
specificity of the naturally-occurring Clostridial toxin from which
the modified Clostridial toxin is derived, at least 90% greater
than the binding specificity of the naturally-occurring Clostridial
toxin from which the modified Clostridial toxin is derived or at
least 100% greater than the binding specificity of the
naturally-occurring Clostridial toxin from which the modified
Clostridial toxin is derived. In aspects of this embodiment, a
modified Clostridial toxin with an increased binding specificity
for a Clostridial toxin receptor exhibits a binding specificity
that is, e.g., at most 10% greater than the binding specificity of
the naturally-occurring Clostridial toxin from which the modified
Clostridial toxin is derived, at most 20% greater than the binding
specificity of the naturally-occurring Clostridial toxin from which
the modified Clostridial toxin is derived, at most 30% greater than
the binding specificity of the naturally-occurring Clostridial
toxin from which the modified Clostridial toxin is derived, at most
40% greater than the binding specificity of the naturally-occurring
Clostridial toxin from which the modified Clostridial toxin is
derived, at most 50% greater than the binding specificity of the
naturally-occurring Clostridial toxin from which the modified
Clostridial toxin is derived, at most 60% greater than the binding
specificity of the naturally-occurring Clostridial toxin from which
the modified Clostridial toxin is derived, at most 70% greater than
the binding specificity of the naturally-occurring Clostridial
toxin from which the modified Clostridial toxin is derived, at most
80% greater than the binding specificity of the naturally-occurring
Clostridial toxin from which the modified Clostridial toxin is
derived, at most 90% greater than the binding specificity of the
naturally-occurring Clostridial toxin from which the modified
Clostridial toxin is derived or at most 100% greater than the
binding specificity of the naturally-occurring Clostridial toxin
from which the modified Clostridial toxin is derived.
[0231] In yet other aspects of this embodiment, a modified
Clostridial toxin with an increased binding specificity for a
Clostridial toxin receptor exhibits a binding specificity that is,
e.g., at least two-fold greater than the binding specificity of the
naturally-occurring Clostridial toxin from which the modified
Clostridial toxin is derived, at least three-fold greater than the
binding specificity of the naturally-occurring Clostridial toxin
from which the modified Clostridial toxin is derived, at least
four-fold greater than the binding specificity of the
naturally-occurring Clostridial toxin from which the modified
Clostridial toxin is derived, at least five-fold greater than the
binding specificity of the naturally-occurring Clostridial toxin
from which the modified Clostridial toxin is derived, at least
ten-fold greater than the binding specificity of the
naturally-occurring Clostridial toxin from which the modified
Clostridial toxin is derived or at least twent.gamma.-fold greater
than the binding specificity of the naturally-occurring Clostridial
toxin from which the modified Clostridial toxin is derived. In yet
other aspects of this embodiment, a modified Clostridial toxin with
an increased binding specificity for a Clostridial toxin receptor
exhibits a binding specificity that is, e.g., at most two-fold
greater than the binding specificity of the naturally-occurring
Clostridial toxin from which the modified Clostridial toxin is
derived, at most three-fold greater than the binding specificity of
the naturally-occurring Clostridial toxin from which the modified
Clostridial toxin is derived, at most four-fold greater than the
binding specificity of the naturally-occurring Clostridial toxin
from which the modified Clostridial toxin is derived, at most
five-fold greater than the binding specificity of the
naturally-occurring Clostridial toxin from which the modified
Clostridial toxin is derived, at most ten-fold greater than the
binding specificity of the naturally-occurring Clostridial toxin
from which the modified Clostridial toxin is derived or at most
twent.gamma.-fold greater than the binding specificity of the
naturally-occurring Clostridial toxin from which the modified
Clostridial toxin is derived.
[0232] As used herein, the term "Clostridial toxin target cell"
means a cell that is a naturally occurring cell that a naturally
occurring Clostridial toxin is capable of intoxicating, including,
without limitation, motor neurons; sensory neurons; autonomic
neurons; such as, e.g., sympathetic neurons and parasympathetic
neurons; non-peptidergic neurons, such as, e.g., cholinergic
neurons, adrenergic neurons, noradrenergic neurons, serotonergic
neurons, GABAergic neurons; and peptidergic neurons, such as, e.g.,
Substance P neurons, Calcitonin Gene Related Peptide neurons,
vasoactive intestinal peptide neurons, Neuropeptide Y neurons,
cholecystokinin neurons.
[0233] An enhanced targeting domain disclosed in the present
specification replaces the binding activity of the Clostridial
toxin targeting domain found in naturally occurring Clostridial
toxins. As used herein, the term "naturally occurring Clostridial
toxin targeting domain" is synonymous with "naturally occurring
Clostridial toxin H.sub.CC targeting domain" and means any
naturally occurring Clostridial toxin polypeptide that can execute
the cell binding step of the intoxication process, including, e.g.,
the binding of the Clostridial toxin to a Clostridial
toxin-specific receptor located on the plasma membrane surface of a
target cell. It is envisioned that replacement of the binding
activity of a naturally occurring Clostridial toxin H.sub.CC
targeting domain by an enhanced targeting domain can be achieved
by, e.g., replacing the entire naturally occurring Clostridial
toxin H.sub.CC targeting domain with an enhanced targeting domain;
replacing a portion of a naturally occurring Clostridial toxin
H.sub.CC targeting domain with an enhanced targeting domain; and
operably-linking an enhanced targeting domain to a naturally
occurring Clostridial toxin comprising a Clostridial toxin H.sub.CC
targeting domain.
[0234] An example of an enhanced targeting domain that increases
binding activity for an endogenous Clostridial toxin receptor
present on a naturally-occurring Clostridial toxin target cell,
includes, without limitation, a modified Clostridial toxin H.sub.CC
targeting domain with enhanced binding activity, such as, e.g., a
modified BoNT/A H.sub.CC targeting domain with enhanced binding
activity, a modified BoNT/B H.sub.CC targeting domain with enhanced
binding activity, a modified BoNT/C1 H.sub.CC targeting domain with
enhanced binding activity, a modified BoNT/D H.sub.CC targeting
domain with enhanced binding activity, a modified BoNT/E H.sub.CC
targeting domain with enhanced binding activity, a modified BoNT/F
H.sub.CC targeting domain with enhanced binding activity, a
modified BoNT/G H.sub.CC targeting domain with enhanced binding
activity and a modified TeNT H.sub.CC targeting domain with
enhanced binding activity. Examples of a modified Clostridial toxin
H.sub.CC targeting domain that increase binding activity include,
e.g., a modified Clostridial toxin H.sub.CC targeting domain that
increases binding affinity for an endogenous Clostridial toxin
receptor present on a naturally-occurring Clostridial toxin target
cell and a modified Clostridial toxin H.sub.CC targeting domain
that increases binding specificity for a subgroup of endogenous
Clostridial toxin receptors present on a naturally-occurring
Clostridial toxin target cell.
[0235] The three-dimensional crystal structures of BoNT/A, BoNT/B
and the H.sub.C domain of TeNT indicate that the carboxyl-terminal
H.sub.CC domain comprises a modified .beta.-trefoil domain which
forms three distinct carbohydrate binding regions that resembles
the carbohydrate binding moiety found in many sugar-binding
proteins, such as, e.g., serum amyloid P, sialidase, cryia,
insecticidal .delta.-endotoxin and lectins. Biochemical studies
indicate that the .beta.-trefoil domain structure of the H.sub.CC
domain appears to mediate the binding to specific carbohydrate
containing components of the Clostridial toxin receptor on the cell
surface, see, e.g., Krzysztof Ginalski et al., Structure-based
Sequence Alignment for the Beta-Trefoil Subdomain of the
Clostridial Neurotoxin Family Provides Residue Level Information
About the Putative Ganglioside Binding Site, 482(1-2) FEBS Lett.
119-124 (2000).
[0236] Proteins containing the structural .beta.-trefoil domain
represents a diverse group of proteins, see, e.g., C. A. Orengo et
al., Protein Superfamilies and Domain Superfolds, 372 Nature
631-634 (1994). The .beta.-trefoil domain comprises a six-stranded
.beta.-barrel closed off at one end by three .beta.-hairpin
structures that exhibits a characteristic pseudo-threefold axis
symmetry. The monomeric structural unit of this three-fold symmetry
is referred to as the .beta.-trefoil fold that contains four
.beta.-sheets organized as a pair of antiparallel .beta.-sheets.
Dividing each of these .beta.-trefoil folds is a .beta.-hairpin
turn. Therefore, in a linear fashion, a .beta.-trefoil domain
comprises four .beta.-sheets of the first .beta.-trefoil fold
(.alpha.-fold), a .beta.-hairpin turn, four .beta.-sheets of the
second .beta.-trefoil fold (.beta.-fold), a second .beta.-hairpin
turn four .beta.-sheets of the third .beta.-trefoil fold
(.gamma.-fold)(see FIG. 2). Because the first hairpin turn is
located between the fourth and fifth .beta.-sheets of the
.beta.-trefoil domain, it is designated the .beta.4/.beta.5
.beta.-hairpin turn. Likewise, since the second hairpin turn is
located between the eight and ninth .beta.-sheets of the
.beta.-trefoil domain, it is designated the .beta.8/.beta.9
.beta.-hairpin turn.
[0237] Continuing research has elucidated that .beta.4/.beta.5 and
.beta.8/.beta.9 .beta.-hairpin turns are important in conferring
the proper pseudo-threefold axis symmetry observed in the
.beta.-trefoil domain. Additionally, this work has demonstrated
that amino acid changes in these two .beta.-hairpin turns can
increase the stability of the .beta.-trefoil domain, which in turn
results in increased binding activity, see, e.g., Stephen R. Brych
et al., Structure and Stability Effects of Mutations Designed to
Increase the Primary Sequence Symmetry Within the Core Region of a
.beta.-trefoil, 10 Protein Sci. 2587-2599 (2001); Jaewon Kim et
al., Alternative Type I and I' Turn Conformations in the
.beta.8/.beta.9 .beta.-hairpin of Human Acidic Fibroblast Growth
Factor, 11 Protein Sci. 459-466 (2002); Jaewon Kim et al., Sequence
swapping Does Not Result in Conformation Swapping for the
.beta.4/.beta.5 and .beta.8/.beta.9 .beta.-hairpin Turns in Human
Acidic Fibroblast Growth Factor, 14 Protein Sci. 351-359 (2005). As
a non-limiting example, replacement of an amino acid comprising
either the .beta.4/.beta.5 hairpin turn or .beta.8/.beta.9
.beta.-hairpin turn with a glycine results in increased
stabilization of the .beta.-trefoil domain. Therefore, replacement
of amino acids located in the .beta.4/.beta.5 and .beta.8/.beta.9
.beta.-hairpin turns of the .beta.-trefoil domains present in the
binding domain of Clostridial toxins will increase binding activity
of such a modified Clostridial toxin by increasing the structural
stability of the .beta.-trefoil domain. The amino acid sequences
comprising the .beta.-trefoil domains found in various Clostridial
toxins are shown in Table 2.
TABLE-US-00002 TABLE 2 .beta.-trefoil Domains of Clostridial Toxins
Amino Acid Sequence Region of Carbohydrate Binding Moieties
.beta.4/.beta.5 .beta.8/.beta.9 Protein SEQ ID NO: .alpha.-fold
.beta.-hairpin turn .beta.-fold .beta.-hairpin turn .gamma.-fold
BoNT/A 1 1111-1162 1163-1178 1179-1223 1224-1236 1237-1296 BoNT/B 2
1098-1147 1148-1165 1166-1210 1211-1222 1223-1291 BoNT/C1 3
1112-1150 1151-1166 1167-1218 1219-1229 1230-1291 BoNT/D 4
1099-1137 1138-1153 1154-1207 1208-1218 1219-1276 BoNT/E 5
1086-1129 1130-1146 1147-1190 1191-1198 1199-1252 BoNT/F 6
1106-1152 1153-1171 1172-1213 1214-1221 1222-1274 BoNT/G 7
1106-1153 1154-1172 1173-1218 1219-1230 1231-1297 TeNT 8 1128-1177
1178-1194 1195-1240 1241-1254 1255-1315
[0238] As is typical for proteins containing a .beta.-trefoil fold,
the overall amino acid sequence identity of the H.sub.CC domain
between Clostridial toxins is low. However, key residues essential
for binding activity have been identified by structural analysis
and mutagenesis experiments, see, e.g., Krzysztof Ginalski et al.,
Structure-based Sequence Alignment for the Beta-Trefoil Subdomain
of the Clostridial Neurotoxin Family Provides Residue Level
Information About the Putative Ganglioside Binding Site, 482(1-2)
FEBS Lett. 119-124 (2000). For example, analysis of the H.sub.CC
domain structure by crystallography identified five highly
conserved residues critical for forming a shallow surface pocket of
a carbohydrate binding moiety. These polar residues make hydrogen
bonds with the carbohydrate ring. In BoNT/A these five polar
residues are Glu 1203, Phe 1252, Ser 1264, Tyr 1267 and Gly 1279,
while in TeNT, these residues are Asp 1222, Thr 1270, Ser 1287, Tyr
1290 and Gly 1300. Additionally, tyrosine residues forming the
hydrophilic wall of this pocket were also important (Trp 1266 of
BoNT/A and Trp 1289 of TeNT) and tryptophan fluorescence quenching
experiments indicated that Trp 1266 of BoNT/A bound carbohydrate
molecules. In another studies, photoaffinity labeling experiments
revealed that Gln 1270 of BoNT/A and His 1293 of TeNT were also
involved in binding carbohydrate molecules. Mutagenesis experiments
designed to assay loss-of-function binding activity mutations
confirmed the importance of many of the residues described above
for BoNT/A and TeNT and extended this analysis to BoNT/B (Glu 1190,
H is 1241, Typ 1262, Tyr 1263), see, e.g., Andreas Rummel et al.,
The H.sub.CC-Domain of Botulinum Neurotoxins A and B Exhibits a
Singular Ganglioside Binding Site Displaying Serotype Specific
Carbohydrate Interaction, 51.beta.) Mol. Microbiol. 631-643
(2004).
[0239] As used herein, the term "Clostridial toxin H.sub.CC
targeting domain" means any naturally occurring Clostridial toxin
polypeptide that can execute the cell binding step of the
intoxication process, including, e.g., the selective binding of the
Clostridial toxin to a toxin-specific receptor located on the
plasma membrane surface of a target cell. As used herein, the term
"modified Clostridial toxin H.sub.CC targeting domain" means a
naturally occurring Clostridial toxin H.sub.CC targeting domain
modified to enhance its cell binding activity for an endogenous
Clostridial toxin receptor, such as, e.g., a binding affinity or a
binding specificity, to a statistically significantly degree
relative to the unmodified naturally occurring Clostridial toxin
H.sub.CC targeting domain from which the modified Clostridial toxin
H.sub.CC targeting domain was derived. By definition, a modified
Clostridial toxin H.sub.CC targeting domain has at least one amino
acid change from the corresponding region of the disclosed
reference sequences (see Table 1) and can be described in percent
identity to the corresponding region of that reference
sequence.
[0240] As another non-limiting examples, a modified BoNT/A H.sub.CC
targeting domain comprising amino acids 1092-1296 of SEQ ID NO: 1
will have at least one amino acid difference, such as, e.g., an
amino acid substitution, deletion or addition, as compared to the
amino acid region 1092-1296 of SEQ ID NO: 1; a modified BoNT/B
H.sub.CC targeting domain comprising amino acids 1079-1291 of SEQ
ID NO: 2 will have at least one amino acid difference, such as,
e.g., an amino acid substitution, deletion or addition, as compared
to the amino acid region 1079-1291 of SEQ ID NO: 2; a modified
BoNT/C1 H.sub.CC targeting domain comprising amino acids 1093-1291
of SEQ ID NO: 3 will have at least one amino acid difference, such
as, e.g., an amino acid substitution, deletion or addition, as
compared to the amino acid region 2093-1291 of SEQ ID NO: 3; a
modified BoNT/D H.sub.CC targeting domain comprising amino acids
1080-1276 of SEQ ID NO: 4 will have at least one amino acid
difference, such as, e.g., an amino acid substitution, deletion or
addition, as compared to the amino acid region 1080-1276 of SEQ ID
NO: 4; a modified BoNT/E H.sub.CC targeting domain comprising amino
acids 1067-1252 of SEQ ID NO: 5 will have at least one amino acid
difference, such as, e.g., an amino acid substitution, deletion or
addition, as compared to the amino acid region 1067-1252 of SEQ ID
NO: 5; a modified BoNT/F H.sub.CC targeting domain comprising amino
acids 1087-1274 of SEQ ID NO: 6 will have at least one amino acid
difference, such as, e.g., an amino acid substitution, deletion or
addition, as compared to the amino acid region 1087-1274 of SEQ ID
NO: 6; a modified BoNT/G H.sub.CC targeting domain comprising amino
acids 1087-1297 of SEQ ID NO: 7 will have at least one amino acid
difference, such as, e.g., an amino acid substitution, deletion or
addition, as compared to the amino acid region 1087-1297 of SEQ ID
NO: 7; and a modified TeNT H.sub.CC targeting domain comprising
amino acids 1109-1315 of SEQ ID NO: 8 will have at least one amino
acid difference, such as, e.g., an amino acid substitution,
deletion or addition, as compared to the amino acid region
1109-1315 of SEQ ID NO: 8.
[0241] It is also envisioned that any of a variety of modified
Clostridial toxin H.sub.CC targeting domain fragments comprising a
binding domain with enhanced binding activity can be useful in
aspects of the present invention with the proviso that these active
fragments can provide enhanced binding activity of the toxin to the
receptor located at the surface of the target cell. The H.sub.CC
targeting domain from the heavy chains of Clostridial toxins are
approximately 165-195 amino acids in length and comprise a binding
domain (Table 1). Research has shown that the entire length of a
H.sub.CC targeting domain from a Clostridial toxin heavy chain is
not necessary for the binding activity of the binding domain. Thus,
aspects of this embodiment can include a Clostridial toxin H.sub.CC
targeting domain comprising a binding domain having a length of,
e.g., at least 150 amino acids, at least 175 amino acids, at least
200 amino acids and at least 225 amino acids. Other aspects of this
embodiment can include a Clostridial toxin H.sub.CC targeting
domain comprising a binding domain having a length of, e.g., at
most 150 amino acids, at most 175 amino acids, at most 200 amino
acids and at most 225 amino acids.
[0242] Any of a variety of sequence alignment methods can be used
to determine percent identity of a modified Clostridial toxin
H.sub.CC targeting domain relative to a naturally-occurring
Clostridial toxin H.sub.CC targeting domain, including, without
limitation, global methods, local methods and hybrid methods, such
as, e.g., segment approach methods. Protocols to determine percent
identity are routine procedures within the scope of one skilled in
the art and from the teaching herein.
[0243] One general approach well known to one skilled in the art on
how to modify a Clostridial toxin H.sub.CC targeting domain in
order to increase its binding activity for a naturally-occurring
Clostridial toxin receptor present on a naturally-occurring
Clostridial toxin target cell involves changing
specifically-identified amino acids. As described above, amino
acids essential to binding activity have been identified and
methods useful for determining which amino acid substitutions will
enhance binding activity are known to one skilled in the art. For
example, recent advances in computational protein design algorithms
have markedly improved capabilities for generating novel proteins
with optimized properties, including, enhanced stability, altered
substrate specificity, improved binding affinity and optimized
pharmacokinetics, see, e.g., Tanja Kortemme et al., Computational
redesign of protein-protein interaction specificity, 11(4) Nat.
Struct. Mol. Biol. 371-379 (2004); Tanja Kortemme and David Baker,
Computational design of protein-protein interactions, 8(1) Curr.
Opin. Chem. Biol. 91-97 (2004); Motomu Shimaoka et al.,
Computational design of an integrin I domain stabilized in the open
high affinity conformation, 7(8) Nat. Struct. Biol. 674-678 (2000);
Loic Martin et al., Rational Design of a CD4 Mimic that Inhibits
HIV-1 Entry and Exposes Cryptic Neutralization Epitopes, 21(1) Nat.
Biotechnol. 71-76 (2003); and Casim A. Sarkar et al., Rational
Cytokine Design for Increased Lifetime and Enhanced Potency Using
pH-activated "Histidine Switching,"20(9) Nat. Biotechnol. 908-913
(2002).
[0244] In addition, methods capable of altering an amino acid
present in a Clostridial toxin H.sub.CC targeting domain, include,
without limitation, site-directed mutagenesis,
oligonucleotide-directed mutagenesis and site-specific mutagenesis.
Non-limiting examples of specific mutagenesis protocols for making
mutations in a Clostridial toxin are described in, e.g.,
Mutagenesis, pp. 13.1-13.105 (Sambrook and Russell, eds., Molecular
Cloning A Laboratory Manual, Vol. 3, 3.sup.rd ed. 2001). In
addition, non-limiting examples of well-characterized mutagenesis
protocols available from commercial vendors include, without
limitation, Altered Sites.RTM. II in vitro Mutagenesis Systems
(Promega Corp., Madison, Wis.); Erase-a-Base.RTM. System (Promega,
Madison, Wis.); GeneTailor.TM. Site-Directed Mutagenesis System
(Invitrogen, Inc., Carlsbad, Calif.); QuikChange.RTM. II
Site-Directed Mutagenesis Kits (Stratagene, La Jolla, Calif.); and
Transformer.TM. Site-Directed Mutagenesis Kit (BD-Clontech,
Mountain View, Calif.).
[0245] Lastly, methods to test the binding activity of modified
Clostridial toxins comprising a modified Clostridial toxin H.sub.CC
targeting domain with altered binding capability are also well
known to one skilled in the art, see, e.g., Lutea A. A. de Jong et
al., Receptor-Binding Assays: Technologies and Applications, 829 J.
Chromatogr. B 1-25 (2005). It is envisioned that heterogeneous
assay types, homogeneous assay types and non-separating homogeneous
assay types can be used to determine the binding activity of a
modified Clostridial toxin with enhanced binding activity disclosed
in the present specification. In a heterogeneous assay, the free
ligand is separated from the bound ligand by, e.g., filtration,
centrifugation or dialysis, before measurement of the binding
activity. In a homogeneous assay, no separation of the free ligand
from the bound ligand is required before measurement of the binding
activity. In non-separating homogeneous assays, either the ligand
or the receptor is immobilized on a solid phase support, in
addition to the no separation aspect of all homogeneous assay.
Non-limiting examples of heterogeneous, homogeneous and
non-separating homogeneous assays include, e.g., radioactive
heterogeneous assays, such as, e.g., filtration assays using
radioactive energy transfer, SPA/flash plate assays using
radioactive energy transfer; and non-radioactive heterogeneous
assays, such as, e.g., filtration assays using fluorescence, FRET
assays using fluorescence energy transfer, FP assays using light
polarization, single-cell FMAT assays and flow cytometry. In
addition, non-limiting examples of well-characterized receptor
binding protocols available from commercial vendors include,
without limitation, Homogeneous Time Resolved Fluorescense-based
receptor binding assays (HTRF.RTM.; Cisbio International, Bedford,
Mass.); DELFIA.RTM.-based receptor binding assays (PerkinElmer
Lifesciences, Boston, Mass.); and AlphaScreen.TM.-based receptor
binding assays (PerkinElmer Lifesciences, Boston, Mass.).
[0246] It is further envisioned that the binding activity of a
modified Clostridial toxin with altered binding capability
disclosed in the present specification can be determined by
affinity chromatography using immobilized receptors and interfacial
optical assays, such as, e.g., total internal reflection
fluorescence (TIRF) and surface plasmon resonance (SPR).
Non-limiting examples of these assays include, e.g., FCS using
diffusion mediated intensity fluctuations, SPR using a
mass-dependent refractive index, TIRF using a mass-independent
refractive index, microarrays using optical intensity changes and
QAC using retention volume.
[0247] As another general approach well known to one skilled in the
art on how to modify a Clostridial toxin H.sub.CC targeting domain
in order to increase its binding activity for a naturally-occurring
Clostridial toxin receptor present on a naturally-occurring
Clostridial toxin target cell involves directed-evolution methods,
see, e.g., Andre Koltermann et al., Process for Generating
Sequence-Specific Proteases by Directed Evolution and Uses Thereof,
U.S. Patent Publication 2004/0072276 (Apr. 15, 2004); Lance E.
Steward and Kei Roger Aoki, Evolved Clostridial Toxins with Altered
Protease Activity, U.S. Patent Publication 2004/0115727 (Jun. 17,
2004); and L Yuan et al., Laboratory-directed protein evolution,
69.beta.) Microbiol. Mol. Biol. Rev. 373-392 (2005).
[0248] Often the first step of directed evolution is error-prone
PCR amplification of the gene of interest or gene recombination
when a family of related genes is available. In this case, the gene
corresponding to full-length Clostridial toxin or the H.sub.CC
targeting domain alone would be amplified under conditions that
yield one to three amino acid substitutions per molecule. The
protein would then be expressed and screened for the desired
activity (i.e., receptor binding and/or enhanced activity). Any
constructs with even a nominal improvement in receptor binding,
regardless of the magnitude of the change, would be submitted for
additional rounds of evolution. As a result of the random
mutagenesis studies, any amino acids displaying improved binding or
even dramatically reduced binding could then be submitted to
saturation mutagenesis. This is a process in which the codon of
interest it completely randomized so that different constructs
containing each of the 20 amino acids substituted at the site of
interest are generated.
[0249] For improvement of receptor binding characteristics, several
different screening approaches could be utilized. If the receptor
is known the soluble portion of the receptor can be expressed
recombinantly for use in SPR binding assays. For example, the
soluble portion of the receptor can be expressed as a fusion to
streptavidin, polyhistidine tag, etc. and the receptor can then be
immobilized on an appropriate sensor chip. Utilizing a SPR
instrument, changes in local refractive index as a result of
receptor binding are measured as a change in the SPR angle. The
rates of change in the SPR angle can then be analyzed to determine
association and dissociation phases and therefore equilibrium
constants. This type of assay could be measured either with the
full-length Clostridial toxin or the H.sub.CC targeting domain.
Additionally, binding type assays relying on affinity pull-down
experiments with affinity tagged receptors acting as a "bait
molecule" could be used. The modified Clostridial toxin or H.sub.CC
targeting domain could then be labeled with radioisotopes for
analysis of the amount of target protein associated with the bait.
Alternatively, radiolabeled ligands can be competed with
Clostridial toxin or H.sub.CC targeting domain variants that are
not labeled.
[0250] If the receptor is not known, variants of full-length
Clostridial toxins can be screened using cells that are known to be
sensitive to treatment with native the Clostridial toxin. In this
case there are several potential readouts for improved receptor
binding, including the presence of the Clostridial toxin in the
cell, measurement of cleaved SNARE protein by, e.g., Western blot
or cell-based FRET activity assay, or inhibition of exocytosis,
e.g., a neurotransmitter release assay.
[0251] Thus, in an embodiment, a modified Clostridial toxin
comprising a targeting domain with enhanced binding activity
comprises a modified Clostridial toxin H.sub.CC targeting domain
with enhanced binding activity. In an aspect of this embodiment, a
modified Clostridial toxin H.sub.CC targeting domain comprises a
modified Clostridial toxin H.sub.CC targeting domain with an
increased binding affinity or a modified Clostridial toxin H.sub.CC
targeting domain fragment with an increased binding affinity. In
another aspect of this embodiment, a modified Clostridial toxin
binding domain comprises a modified Clostridial toxin H.sub.CC
targeting domain with an increased binding specificity or a
modified Clostridial toxin H.sub.CC targeting domain fragment with
an increased binding specificity.
[0252] In another embodiment, a modified Clostridial toxin
comprising a targeting domain with enhanced binding activity
comprises a modified BoNT/A H.sub.CC targeting domain with enhanced
binding activity. In an aspect of this embodiment, a modified
Clostridial toxin comprises a modified BoNT/A H.sub.CC targeting
domain with an increased binding affinity or a modified BoNT/A
H.sub.CC targeting domain fragment with an increased binding
affinity. In another aspect of this embodiment, a modified
Clostridial toxin comprises a modified BoNT/A H.sub.CC targeting
domain with an increased binding specificity or a modified BoNT/A
H.sub.CC targeting domain fragment with an increased binding
specificity.
[0253] In another embodiment, a modified BoNT/A H.sub.CC targeting
domain with enhanced binding activity comprises a modification of
amino acids 1111-1296 of SEQ ID NO: 1. In another aspect of this
embodiment, a modified BoNT/A H.sub.CC targeting domain with an
altered cell binding capability comprises a modified .alpha.-fold
motif of a .beta.-trefoil domain of a BoNT/A H.sub.CC targeting
domain, a modified .beta.-fold motif of a .beta.-trefoil domain of
a BoNT/A H.sub.CC targeting domain, or a modified .gamma.-fold
motif of a .beta.-trefoil domain of a BoNT/A H.sub.CC targeting
domain. In another aspect of this embodiment, a modified BoNT/A
H.sub.CC targeting domain with an altered cell binding capability
comprises a modification to amino acids 1111-1162, amino acids
1179-1223, or amino acids 1237-1296 of SEQ ID NO: 1.
[0254] In other aspects of this embodiment, a modified BoNT/A
H.sub.CC targeting domain comprising a .beta.-trefoil fold domain
with enhanced binding activity comprises a polypeptide having,
e.g., at least 70% amino acid identity with amino acids 1111-1162,
amino acids 1179-1223, or amino acids 1237-1296 of SEQ ID NO: 1, at
least 75% amino acid identity with amino acids 1111-1162, amino
acids 1179-1223, or amino acids 1237-1296 of SEQ ID NO: 1, at least
80% amino acid identity with amino acids 1111-1162, amino acids
1179-1223, or amino acids 1237-1296 of SEQ ID NO: 1, at least 85%
amino acid identity with amino acids 1111-1162, amino acids
1179-1223, or amino acids 1237-1296 of SEQ ID NO: 1, at least 90%
amino acid identity with amino acids 1111-1162, amino acids
1179-1223, or amino acids 1237-1296 of SEQ ID NO: 1 or at least 95%
amino acid identity with amino acids 1111-1162, amino acids
1179-1223, or amino acids 1237-1296 of SEQ ID NO: 1. In yet other
aspects of this embodiment, a modified BoNT/A H.sub.CC targeting
domain comprising a .beta.-trefoil fold domain with enhanced
binding activity comprises a polypeptide having, e.g., at most 70%
amino acid identity with amino acids 1111-1162, amino acids
1179-1223, or amino acids 1237-1296 of SEQ ID NO: 1, at most 75%
amino acid identity with amino acids 1111-1162, amino acids
1179-1223, or amino acids 1237-1296 of SEQ ID NO: 1, at most 80%
amino acid identity with amino acids 1111-1162, amino acids
1179-1223, or amino acids 1237-1296 of SEQ ID NO: 1, at most 85%
amino acid identity with amino acids 1111-1162, amino acids
1179-1223, or amino acids 1237-1296 of SEQ ID NO: 1, at most 90%
amino acid identity with amino acids 1111-1162, amino acids
1179-1223, or amino acids 1237-1296 of SEQ ID NO: 1 or at most 95%
amino acid identity with amino acids 1111-1162, amino acids
1179-1223, or amino acids 1237-1296 of SEQ ID NO: 1.
[0255] In other aspects of this embodiment, a modified BoNT/A
H.sub.CC targeting domain comprising a .beta.-trefoil fold domain
with enhanced binding activity comprises a polypeptide having,
e.g., at most one, two, three, four, five, six, seven, eight, nine,
10 or 20 non-contiguous amino acid substitutions relative to amino
acids 1111-1162, amino acids 1179-1223, or amino acids 1237-1296 of
SEQ ID NO: 1. In other aspects of this embodiment, a modified
BoNT/A H.sub.CC targeting domain with enhanced binding activity
comprises a polypeptide having, e.g., at least one, two, three,
four, five, six, seven, eight, nine, 10 or 20 non-contiguous amino
acid substitutions relative to amino acids 1111-1162, amino acids
1179-1223, or amino acids 1237-1296 of SEQ ID NO: 1. In yet other
aspects of this embodiment, a modified BoNT/A H.sub.CC targeting
domain comprising a .beta.-trefoil fold domain with enhanced
binding activity comprises a polypeptide having, e.g., at most one,
two, three, four, five, six, seven, eight, nine, 10 or 20
non-contiguous amino acid deletions relative to amino acids
1111-1162, amino acids 1179-1223, or amino acids 1237-1296 of SEQ
ID NO: 1. In other aspects of this embodiment, a modified BoNT/A
H.sub.CC targeting domain comprising a .beta.-trefoil fold domain
with enhanced binding activity comprises a polypeptide having,
e.g., at least one, two, three, four, five, six, seven, eight,
nine, 10 or 20 non-contiguous amino acid deletions relative to
amino acids 1111-1162, amino acids 1179-1223, or amino acids
1237-1296 of SEQ ID NO: 1. In still other aspects of this
embodiment, a modified BoNT/A H.sub.CC targeting domain comprising
a .beta.-trefoil fold domain with enhanced binding activity
comprises a polypeptide having, e.g., at most one, two, three,
four, five, six, seven, eight, nine, 10 or 20 non-contiguous amino
acid additions relative to amino acids 1111-1162, amino acids
1179-1223, or amino acids 1237-1296 of SEQ ID NO: 1. In other
aspects of this embodiment, a modified BoNT/A H.sub.CC targeting
domain comprising a .beta.-trefoil fold domain with enhanced
binding activity comprises a polypeptide having, e.g., at least
one, two, three, four, five, six, seven, eight, nine, 10 or 20
non-contiguous amino acid additions relative to amino acids
1111-1162, amino acids 1179-1223, or amino acids 1237-1296 of SEQ
ID NO: 1.
[0256] In other aspects of this embodiment, a modified BoNT/A
H.sub.CC targeting domain comprising a .beta.-trefoil fold domain
with enhanced binding activity comprises a polypeptide having,
e.g., at most one, two, three, four, five, six, seven, eight, nine,
10 or 20 contiguous amino acid substitutions relative to amino
acids 1111-1162, amino acids 1179-1223, or amino acids 1237-1296 of
SEQ ID NO: 1. In other aspects of this embodiment, a modified
BoNT/A H.sub.CC targeting domain comprising a .beta.-trefoil fold
domain with enhanced binding activity comprises a polypeptide
having, e.g., at least one, two, three, four, five, six, seven,
eight, nine, 10 or 20 contiguous amino acid substitutions relative
to amino acids 1111-1162, amino acids 1179-1223, or amino acids
1237-1296 of SEQ ID NO: 1. In yet other aspects of this embodiment,
a modified BoNT/A H.sub.CC targeting domain comprising a
.beta.-trefoil fold domain with enhanced binding activity comprises
a polypeptide having, e.g., at most one, two, three, four, five,
six, seven, eight, nine, 10 or 20 contiguous amino acid deletions
relative to amino acids 1111-1162, amino acids 1179-1223, or amino
acids 1237-1296 of SEQ ID NO: 1. In other aspects of this
embodiment, a modified BoNT/A H.sub.CC targeting domain comprising
a .beta.-trefoil fold domain with enhanced binding activity
comprises a polypeptide having, e.g., at least one, two, three,
four, five, six, seven, eight, nine, 10 or 20 contiguous amino acid
deletions relative to amino acids 1111-1162, amino acids 1179-1223,
or amino acids 1237-1296 of SEQ ID NO: 1. In still other aspects of
this embodiment, a modified BoNT/A H.sub.CC targeting domain
comprising a .beta.-trefoil fold domain with enhanced binding
activity comprises a polypeptide having, e.g., at most one, two,
three, four, five, six, seven, eight, nine, 10 or 20 contiguous
amino acid additions relative to amino acids 1111-1162, amino acids
1179-1223, or amino acids 1237-1296 of SEQ ID NO: 1. In other
aspects of this embodiment, a modified BoNT/A H.sub.CC targeting
domain comprising a .beta.-trefoil fold domain with enhanced
binding activity comprises a polypeptide having, e.g., at least
one, two, three, four, five, six, seven, eight, nine, 10 or 20
contiguous amino acid additions relative to amino acids 1111-1162,
amino acids 1179-1223, or amino acids 1237-1296 of SEQ ID NO:
1.
[0257] In another embodiment, a modified BoNT/A H.sub.CC targeting
domain with enhanced binding activity comprises a modified BoNT/A
H.sub.CC targeting domain with enhanced binding activity of
comprises a modified .beta.4/.beta.5 hairpin turn of a
.beta.-trefoil domain of a BoNT/A H.sub.CC targeting domain or a
.beta.8/.beta.9 hairpin turn of a .beta.-trefoil domain of a BoNT/A
H.sub.CC targeting domain. In another aspect of this embodiment, a
modified BoNT/A H.sub.CC targeting domain with enhanced binding
activity comprises a modification of amino acids 1163-1178 or amino
acids 1224-1236 of SEQ ID NO: 1.
[0258] In other aspects of this embodiment, a modified BoNT/A
H.sub.CC targeting domain comprising a .beta.-trefoil fold domain
with enhanced binding activity comprises a polypeptide having,
e.g., at least 70% amino acid identity with amino acids 1163-1178
or amino acids 1224-1236 of SEQ ID NO: 1, at least 75% amino acid
identity with amino acids 1163-1178 or amino acids 1224-1236 of SEQ
ID NO: 1, at least 80% amino acid identity with amino acids
1163-1178 or amino acids 1224-1236 of SEQ ID NO: 1, at least 85%
amino acid identity with amino acids 1163-1178 or amino acids
1224-1236 of SEQ ID NO: 1, at least 90% amino acid identity with
amino acids 1163-1178 or amino acids 1224-1236 of SEQ ID NO: 1 or
at least 95% amino acid identity with amino acids 1163-1178 or
amino acids 1224-1236 of SEQ ID NO: 1. In yet other aspects of this
embodiment, a modified BoNT/A H.sub.CC targeting domain comprising
a .beta.-trefoil fold domain with enhanced binding activity
comprises a polypeptide having, e.g., at most 70% amino acid
identity with amino acids 1163-1178 or amino acids 1224-1236 of SEQ
ID NO: 1, at most 75% amino acid identity with amino acids
1163-1178 or amino acids 1224-1236 of SEQ ID NO: 1, at most 80%
amino acid identity with amino acids 1163-1178 or amino acids
1224-1236 of SEQ ID NO: 1, at most 85% amino acid identity with
amino acids 1163-1178 or amino acids 1224-1236 of SEQ ID NO: 1, at
most 90% amino acid identity with amino acids 1163-1178 or amino
acids 1224-1236 of SEQ ID NO: 1 or at most 95% amino acid identity
with amino acids 1163-1178 or amino acids 1224-1236 of SEQ ID NO:
1.
[0259] In other aspects of this embodiment, a modified BoNT/A
H.sub.CC targeting domain comprising a .beta.-trefoil fold domain
with enhanced binding activity comprises a polypeptide having,
e.g., at most one, two, three, four, five, six, seven, eight, nine
or 10 non-contiguous amino acid substitutions relative to amino
acids 1163-1178 or amino acids 1224-1236 of SEQ ID NO: 1. In other
aspects of this embodiment, a modified BoNT/A H.sub.CC targeting
domain comprises a polypeptide having, e.g., at least one, two,
three, four, five, six, seven, eight, nine or 10 non-contiguous
amino acid substitutions relative to amino acids 1163-1178 or amino
acids 1224-1236 of SEQ ID NO: 1. In other aspects of this
embodiment, a non-contiguous amino acid substitution of any amino
acid from amino acids 1163-1178 or amino acids 1224-1236 of SEQ ID
NO: 1 can be replaced with glycine. In other aspects of this
embodiment, a non-contiguous amino acid substitution of any
hydrophobic amino acid from amino acids 1163-1178 or amino acids
1224-1236 of SEQ ID NO: 1 can be replaced with phenylalanine. In
yet other aspects of this embodiment, a modified BoNT/A H.sub.CC
targeting domain comprising a .beta.-trefoil fold domain with
enhanced binding activity comprises a polypeptide having, e.g., at
most one, two, three, four, five, six, seven, eight, nine or 10
non-contiguous amino acid deletions relative to amino acids
1163-1178 or amino acids 1224-1236 of SEQ ID NO: 1. In other
aspects of this embodiment, a modified BoNT/A H.sub.CC targeting
domain comprising a .beta.-trefoil fold domain with enhanced
binding activity comprises a polypeptide having, e.g., at least
one, two, three, four, five, six, seven, eight, nine or 10
non-contiguous amino acid deletions relative to amino acids
1163-1178 or amino acids 1224-1236 of SEQ ID NO: 1. In still other
aspects of this embodiment, a modified BoNT/A H.sub.CC targeting
domain comprising a .beta.-trefoil fold domain with enhanced
binding activity comprises a polypeptide having, e.g., at most one,
two, three, four, five, six, seven, eight, nine or 10
non-contiguous amino acid additions relative to amino acids
1163-1178 or amino acids 1224-1236 of SEQ ID NO: 1. In other
aspects of this embodiment, a modified BoNT/A H.sub.CC targeting
domain comprising a .beta.-trefoil fold domain with enhanced
binding activity comprises a polypeptide having, e.g., at least
one, two, three, four, five, six, seven, eight, nine or 10
non-contiguous amino acid additions relative to amino acids
1163-1178 or amino acids 1224-1236 of SEQ ID NO: 1.
[0260] In other aspects of this embodiment, a modified BoNT/A
H.sub.CC targeting domain comprising a .beta.-trefoil fold domain
with enhanced binding activity comprises a polypeptide having,
e.g., at most one, two, three, four, five, six, seven, eight, nine
or 10 contiguous amino acid substitutions relative to amino acids
1163-1178 or amino acids 1224-1236 of SEQ ID NO: 1. In other
aspects of this embodiment, a modified BoNT/A H.sub.CC targeting
domain comprising a .beta.-trefoil fold domain with enhanced
binding activity comprises a polypeptide having, e.g., at least
one, two, three, four, five, six, seven, eight, nine or 10
contiguous amino acid substitutions relative to amino acids
1163-1178 or amino acids 1224-1236 of SEQ ID NO: 1. In other
aspects of this embodiment, contiguous amino acid substitutions of
amino acids from amino acids 1163-1178 or amino acids 1224-1236 of
SEQ ID NO: 1 can be replaced with glycine. In other aspects of this
embodiment, contiguous amino acid substitutions of hydrophobic
amino acids from amino acids 1163-1178 or amino acids 1224-1236 of
SEQ ID NO: 1 can be replaced with phenylalanine. In yet other
aspects of this embodiment, a modified BoNT/A H.sub.CC targeting
domain comprising a .beta.-trefoil fold domain with enhanced
binding activity comprises a polypeptide having, e.g., at most one,
two, three, four, five, six, seven, eight, nine or 10 contiguous
amino acid deletions relative to amino acids 1163-1178 or amino
acids 1224-1236 of SEQ ID NO: 1. In other aspects of this
embodiment, a modified BoNT/A H.sub.CC targeting domain comprising
a .beta.-trefoil fold domain with enhanced binding activity
comprises a polypeptide having, e.g., at least one, two, three,
four, five, six, seven, eight, nine or 10 contiguous amino acid
deletions relative to amino acids 1163-1178 or amino acids
1224-1236 of SEQ ID NO: 1. In still other aspects of this
embodiment, a modified BoNT/A H.sub.CC targeting domain comprising
a .beta.-trefoil fold domain with enhanced binding activity
comprises a polypeptide having, e.g., at most one, two, three,
four, five, six, seven, eight, nine or 10 contiguous amino acid
additions relative to amino acids 1163-1178 or amino acids
1224-1236 of SEQ ID NO: 1. In other aspects of this embodiment, a
modified BoNT/A H.sub.CC targeting domain comprising a
.beta.-trefoil fold domain with enhanced binding activity comprises
a polypeptide having, e.g., at least one, two, three, four, five,
six, seven, eight, nine or 10 contiguous amino acid additions
relative to amino acids 1163-1178 or amino acids 1224-1236 of SEQ
ID NO: 1.
[0261] In other aspects of this embodiment, a modified BoNT/A
H.sub.CC targeting domain with enhanced binding activity comprises
a substitution of amino acid Trp 1101, Gly 1102, Leu 1105, Tyr
1111, Tyr 1112, Gly 1158, Ile 1163, Asp 1179, Glu 1203, Phe 1252,
Ser 1264, Trp 1266, Tyr 1267, Gln 1270, Gly 1279 or Trp 1282, or
any combination thereof, the substitution enhancing the binding
activity of the modified BoNT/A H.sub.CC targeting domain. In other
aspects of this embodiment, a modified BoNT/A H.sub.CC targeting
domain with enhanced binding activity comprises a deletion of amino
acid Trp 1101, Gly 1102, Leu 1105, Tyr 1111, Tyr 1112, Gly 1158,
Ile 1163, Asp 1179, Glu 1203, Phe 1252, Ser 1264, Trp 1266, Tyr
1267, Gln 1270, Gly 1279 or Trp 1282, or any combination thereof,
the deletion enhancing the binding activity of the modified BoNT/A
H.sub.CC targeting domain.
[0262] In another embodiment, a modified Clostridial toxin
comprising a targeting domain with enhanced binding activity
comprises a modified BoNT/B H.sub.CC targeting domain with enhanced
binding activity. In an aspect of this embodiment, a modified
Clostridial toxin comprises a modified BoNT/B H.sub.CC targeting
domain with an increased binding affinity or a modified BoNT/B
H.sub.CC targeting domain fragment with an increased binding
affinity. In another aspect of this embodiment, a modified
Clostridial toxin comprises a modified BoNT/B H.sub.CC targeting
domain with an increased binding specificity or a modified BoNT/B
H.sub.CC targeting domain fragment with an increased binding
specificity.
[0263] In another embodiment, a modified BoNT/B H.sub.CC targeting
domain with enhanced binding activity comprises a modification of
amino acids 1098-1291 of SEQ ID NO: 2. In another aspect of this
embodiment, a modified BoNT/B H.sub.CC targeting domain with an
altered cell binding capability comprises a modified .alpha.-fold
motif of a .beta.-trefoil domain of a BoNT/B H.sub.CC targeting
domain, a modified .beta.-fold motif of a .beta.-trefoil domain of
a BoNT/B H.sub.CC targeting domain, or a modified .gamma.-fold
motif of a .beta.-trefoil domain of a BoNT/B H.sub.CC targeting
domain. In another aspect of this embodiment, a modified BoNT/B
H.sub.CC targeting domain with an altered cell binding capability
comprises a modification to amino acids 1098-1147, amino acids
1166-1210, or amino acids 1223-1291 of SEQ ID NO: 2.
[0264] In other aspects of this embodiment, a modified BoNT/B
H.sub.CC targeting domain comprising a .beta.-trefoil fold domain
with enhanced binding activity comprises a polypeptide having,
e.g., at least 70% amino acid identity with amino acids 1098-1147,
amino acids 1166-1210, or amino acids 1223-1291 of SEQ ID NO: 2, at
least 75% amino acid identity with amino acids 1098-1147, amino
acids 1166-1210, or amino acids 1223-1291 of SEQ ID NO: 2, at least
80% amino acid identity with amino acids 1098-1147, amino acids
1166-1210, or amino acids 1223-1291 of SEQ ID NO: 2, at least 85%
amino acid identity with amino acids 1098-1147, amino acids
1166-1210, or amino acids 1223-1291 of SEQ ID NO: 2, at least 90%
amino acid identity with amino acids 1098-1147, amino acids
1166-1210, or amino acids 1223-1291 of SEQ ID NO: 2 or at least 95%
amino acid identity with amino acids 1098-1147, amino acids
1166-1210, or amino acids 1223-1291 of SEQ ID NO: 2. In yet other
aspects of this embodiment, a modified BoNT/B H.sub.CC targeting
domain comprising a .beta.-trefoil fold domain with enhanced
binding activity comprises a polypeptide having, e.g., at most 70%
amino acid identity with amino acids 1098-1147, amino acids
1166-1210, or amino acids 1223-1291 of SEQ ID NO: 2, at most 75%
amino acid identity with amino acids 1098-1147, amino acids
1166-1210, or amino acids 1223-1291 of SEQ ID NO: 2, at most 80%
amino acid identity with amino acids 1098-1147, amino acids
1166-1210, or amino acids 1223-1291 of SEQ ID NO: 2, at most 85%
amino acid identity with amino acids 1098-1147, amino acids
1166-1210, or amino acids 1223-1291 of SEQ ID NO: 2, at most 90%
amino acid identity with amino acids 1098-1147, amino acids
1166-1210, or amino acids 1223-1291 of SEQ ID NO: 2 or at most 95%
amino acid identity with amino acids 1098-1147, amino acids
1166-1210, or amino acids 1223-1291 of SEQ ID NO: 2.
[0265] In other aspects of this embodiment, a modified BoNT/B
H.sub.CC targeting domain comprising a .beta.-trefoil fold domain
with enhanced binding activity comprises a polypeptide having,
e.g., at most one, two, three, four, five, six, seven, eight, nine,
10 or 20 non-contiguous amino acid substitutions relative to amino
acids 1098-1147, amino acids 1166-1210, or amino acids 1223-1291 of
SEQ ID NO: 2. In other aspects of this embodiment, a modified
BoNT/B H.sub.CC targeting domain with enhanced binding activity
comprises a polypeptide having, e.g., at least one, two, three,
four, five, six, seven, eight, nine, 10 or 20 non-contiguous amino
acid substitutions relative to amino acids 1098-1147, amino acids
1166-1210, or amino acids 1223-1291 of SEQ ID NO: 2. In yet other
aspects of this embodiment, a modified BoNT/B H.sub.CC targeting
domain comprising a .beta.-trefoil fold domain with enhanced
binding activity comprises a polypeptide having, e.g., at most one,
two, three, four, five, six, seven, eight, nine, 10 or 20
non-contiguous amino acid deletions relative to amino acids
1098-1147, amino acids 1166-1210, or amino acids 1223-1291 of SEQ
ID NO: 2. In other aspects of this embodiment, a modified BoNT/B
H.sub.CC targeting domain comprising a .beta.-trefoil fold domain
with enhanced binding activity comprises a polypeptide having,
e.g., at least one, two, three, four, five, six, seven, eight,
nine, 10 or 20 non-contiguous amino acid deletions relative to
amino acids 1098-1147, amino acids 1166-1210, or amino acids
1223-1291 of SEQ ID NO: 2. In still other aspects of this
embodiment, a modified BoNT/B H.sub.CC targeting domain comprising
a .beta.-trefoil fold domain with enhanced binding activity
comprises a polypeptide having, e.g., at most one, two, three,
four, five, six, seven, eight, nine, 10 or 20 non-contiguous amino
acid additions relative to amino acids 1098-1147, amino acids
1166-1210, or amino acids 1223-1291 of SEQ ID NO: 2. In other
aspects of this embodiment, a modified BoNT/B H.sub.CC targeting
domain comprising a .beta.-trefoil fold domain with enhanced
binding activity comprises a polypeptide having, e.g., at least
one, two, three, four, five, six, seven, eight, nine, 10 or 20
non-contiguous amino acid additions relative to amino acids
1098-1147, amino acids 1166-1210, or amino acids 1223-1291 of SEQ
ID NO: 2.
[0266] In other aspects of this embodiment, a modified BoNT/B
H.sub.CC targeting domain comprising a 11-trefoil fold domain with
enhanced binding activity comprises a polypeptide having, e.g., at
most one, two, three, four, five, six, seven, eight, nine, 10 or 20
contiguous amino acid substitutions relative to amino acids
1098-1147, amino acids 1166-1210, or amino acids 1223-1291 of SEQ
ID NO: 2. In other aspects of this embodiment, a modified BoNT/B
H.sub.CC targeting domain comprising a .beta.-trefoil fold domain
with enhanced binding activity comprises a polypeptide having,
e.g., at least one, two, three, four, five, six, seven, eight,
nine, 10 or 20 contiguous amino acid substitutions relative to
amino acids 1098-1147, amino acids 1166-1210, or amino acids
1223-1291 of SEQ ID NO: 2. In yet other aspects of this embodiment,
a modified BoNT/B H.sub.CC targeting domain comprising a
.beta.-trefoil fold domain with enhanced binding activity comprises
a polypeptide having, e.g., at most one, two, three, four, five,
six, seven, eight, nine, 10 or 20 contiguous amino acid deletions
relative to amino acids 1098-1147, amino acids 1166-1210, or amino
acids 1223-1291 of SEQ ID NO: 2. In other aspects of this
embodiment, a modified BoNT/B H.sub.CC targeting domain comprising
a .beta.-trefoil fold domain with enhanced binding activity
comprises a polypeptide having, e.g., at least one, two, three,
four, five, six, seven, eight, nine, 10 or 20 contiguous amino acid
deletions relative to amino acids 1098-1147, amino acids 1166-1210,
or amino acids 1223-1291 of SEQ ID NO: 2. In still other aspects of
this embodiment, a modified BoNT/B H.sub.CC targeting domain
comprising a .beta.-trefoil fold domain with enhanced binding
activity comprises a polypeptide having, e.g., at most one, two,
three, four, five, six, seven, eight, nine, 10 or 20 contiguous
amino acid additions relative to amino acids 1098-1147, amino acids
1166-1210, or amino acids 1223-1291 of SEQ ID NO: 2. In other
aspects of this embodiment, a modified BoNT/B H.sub.CC targeting
domain comprising a .beta.-trefoil fold domain with enhanced
binding activity comprises a polypeptide having, e.g., at least
one, two, three, four, five, six, seven, eight, nine, 10 or 20
contiguous amino acid additions relative to amino acids 1098-1147,
amino acids 1166-1210, or amino acids 1223-1291 of SEQ ID NO:
2.
[0267] In another embodiment, a modified BoNT/B H.sub.CC targeting
domain with enhanced binding activity comprises a modified BoNT/B
H.sub.CC targeting domain with enhanced binding activity of
comprises a modified .beta.4/.beta.5 hairpin turn of a
.beta.-trefoil domain of a BoNT/B H.sub.CC targeting domain or a
.beta.8/.beta.9 hairpin turn of a .beta.-trefoil domain of a BoNT/B
H.sub.CC targeting domain. In another aspect of this embodiment, a
modified BoNT/B H.sub.CC targeting domain with enhanced binding
activity comprises a modification of amino acids 1148-1165 or amino
acids 1211-1222 of SEQ ID NO: 2.
[0268] In other aspects of this embodiment, a modified BoNT/B
H.sub.CC targeting domain comprising a .beta.-trefoil fold domain
with enhanced binding activity comprises a polypeptide having,
e.g., at least 70% amino acid identity with amino acids 1148-1165
or amino acids 1211-1222 of SEQ ID NO: 2, at least 75% amino acid
identity with amino acids 1148-1165 or amino acids 1211-1222 of SEQ
ID NO: 2, at least 80% amino acid identity with amino acids
1148-1165 or amino acids 1211-1222 of SEQ ID NO: 2, at least 85%
amino acid identity with amino acids 1148-1165 or amino acids
1211-1222 of SEQ ID NO: 2, at least 90% amino acid identity with
amino acids 1148-1165 or amino acids 1211-1222 of SEQ ID NO: 2 or
at least 95% amino acid identity with amino acids 1148-1165 or
amino acids 1211-1222 of SEQ ID NO: 2. In yet other aspects of this
embodiment, a modified BoNT/B H.sub.CC targeting domain comprising
a .beta.-trefoil fold domain with enhanced binding activity
comprises a polypeptide having, e.g., at most 70% amino acid
identity with amino acids 1148-1165 or amino acids 1211-1222 of SEQ
ID NO: 2, at most 75% amino acid identity with amino acids
1148-1165 or amino acids 1211-1222 of SEQ ID NO: 2, at most 80%
amino acid identity with amino acids 1148-1165 or amino acids
1211-1222 of SEQ ID NO: 2, at most 85% amino acid identity with
amino acids 1148-1165 or amino acids 1211-1222 of SEQ ID NO: 2, at
most 90% amino acid identity with amino acids 1148-1165 or amino
acids 1211-1222 of SEQ ID NO: 2 or at most 95% amino acid identity
with amino acids 1148-1165 or amino acids 1211-1222 of SEQ ID NO:
2.
[0269] In other aspects of this embodiment, a modified BoNT/B
H.sub.CC targeting domain comprising a .beta.-trefoil fold domain
with enhanced binding activity comprises a polypeptide having,
e.g., at most one, two, three, four, five, six, seven, eight, nine
or 10 non-contiguous amino acid substitutions relative to amino
acids 1148-1165 or amino acids 1211-1222 of SEQ ID NO: 2. In other
aspects of this embodiment, a modified BoNT/B H.sub.CC targeting
domain comprises a polypeptide having, e.g., at least one, two,
three, four, five, six, seven, eight, nine or 10 non-contiguous
amino acid substitutions relative to amino acids 1148-1165 or amino
acids 1211-1222 of SEQ ID NO: 2. In other aspects of this
embodiment, a non-contiguous amino acid substitution of any amino
acid from amino acids 1148-1165 or amino acids 1211-1222 of SEQ ID
NO: 2 can be replaced with glycine. In other aspects of this
embodiment, a non-contiguous amino acid substitution of any
hydrophobic amino acid from amino acids 1148-1165 or amino acids
1211-1222 of SEQ ID NO: 2 can be replaced with phenylalanine. In
yet other aspects of this embodiment, a modified BoNT/B H.sub.CC
targeting domain comprising a .beta.-trefoil fold domain with
enhanced binding activity comprises a polypeptide having, e.g., at
most one, two, three, four, five, six, seven, eight, nine or 10
non-contiguous amino acid deletions relative to amino acids
1148-1165 or amino acids 1211-1222 of SEQ ID NO: 2. In other
aspects of this embodiment, a modified BoNT/B H.sub.CC targeting
domain comprising a .beta.-trefoil fold domain with enhanced
binding activity comprises a polypeptide having, e.g., at least
one, two, three, four, five, six, seven, eight, nine or 10
non-contiguous amino acid deletions relative to amino acids
1148-1165 or amino acids 1211-1222 of SEQ ID NO: 2. In still other
aspects of this embodiment, a modified BoNT/B H.sub.CC targeting
domain comprising a .beta.-trefoil fold domain with enhanced
binding activity comprises a polypeptide having, e.g., at most one,
two, three, four, five, six, seven, eight, nine or 10
non-contiguous amino acid additions relative to amino acids
1148-1165 or amino acids 1211-1222 of SEQ ID NO: 2. In other
aspects of this embodiment, a modified BoNT/B H.sub.CC targeting
domain comprising a .beta.-trefoil fold domain with enhanced
binding activity comprises a polypeptide having, e.g., at least
one, two, three, four, five, six, seven, eight, nine or 10
non-contiguous amino acid additions relative to amino acids
1148-1165 or amino acids 1211-1222 of SEQ ID NO: 2.
[0270] In other aspects of this embodiment, a modified BoNT/B
H.sub.CC targeting domain comprising a 11-trefoil fold domain with
enhanced binding activity comprises a polypeptide having, e.g., at
most one, two, three, four, five, six, seven, eight, nine or 10
contiguous amino acid substitutions relative to amino acids
1148-1165 or amino acids 1211-1222 of SEQ ID NO: 2. In other
aspects of this embodiment, a modified BoNT/B H.sub.CC targeting
domain comprising a .beta.-trefoil fold domain with enhanced
binding activity comprises a polypeptide having, e.g., at least
one, two, three, four, five, six, seven, eight, nine or 10
contiguous amino acid substitutions relative to amino acids
1148-1165 or amino acids 1211-1222 of SEQ ID NO: 2. In other
aspects of this embodiment, contiguous amino acid substitutions of
amino acids from amino acids 1148-1165 or amino acids 1211-1222 of
SEQ ID NO: 2 can be replaced with glycine. In other aspects of this
embodiment, contiguous amino acid substitutions of hydrophobic
amino acids from amino acids 1148-1165 or amino acids 1211-1222 of
SEQ ID NO: 2 can be replaced with phenylalanine. In yet other
aspects of this embodiment, a modified BoNT/B H.sub.CC targeting
domain comprising a .beta.-trefoil fold domain with enhanced
binding activity comprises a polypeptide having, e.g., at most one,
two, three, four, five, six, seven, eight, nine or 10 contiguous
amino acid deletions relative to amino acids 1148-1165 or amino
acids 1211-1222 of SEQ ID NO: 2. In other aspects of this
embodiment, a modified BoNT/B H.sub.CC targeting domain comprising
a .beta.-trefoil fold domain with enhanced binding activity
comprises a polypeptide having, e.g., at least one, two, three,
four, five, six, seven, eight, nine or 10 contiguous amino acid
deletions relative to amino acids 1148-1165 or amino acids
1211-1222 of SEQ ID NO: 2. In still other aspects of this
embodiment, a modified BoNT/B H.sub.CC targeting domain comprising
a .beta.-trefoil fold domain with enhanced binding activity
comprises a polypeptide having, e.g., at most one, two, three,
four, five, six, seven, eight, nine or 10 contiguous amino acid
additions relative to amino acids 1148-1165 or amino acids
1211-1222 of SEQ ID NO: 2. In other aspects of this embodiment, a
modified BoNT/B H.sub.CC targeting domain comprising a
.beta.-trefoil fold domain with enhanced binding activity comprises
a polypeptide having, e.g., at least one, two, three, four, five,
six, seven, eight, nine or 10 contiguous amino acid additions
relative to amino acids 1148-1165 or amino acids 1211-1222 of SEQ
ID NO: 2.
[0271] In other aspects of this embodiment, a modified BoNT/B
H.sub.CC targeting domain with enhanced binding activity comprises
a substitution of amino acid Trp 1088, Gly 1089, Leu 1092, Tyr
1098, Tyr 1099, Gly 1142, Ile 1147, Asp 1165, Glu 1191, Ile 1240,
Ser 1260, Trp 1262, Tyr 1263, Glu 1266, Gly 1277 or Trp 1280, or
any combination thereof, the substitution enhancing the binding
capability of the modified BoNT/B H.sub.CC targeting domain. In
other aspects of this embodiment, a modified BoNT/B H.sub.CC
targeting domain with enhanced binding activity comprises a
deletion of amino acid Trp 1088, Gly 1089, Leu 1092, Tyr 1098, Tyr
1099, Gly 1142, Ile 1147, Asp 1165, Glu 1191, Ile 1240, Ser 1260,
Trp 1262, Tyr 1263, Glu 1266, Gly 1277 or Trp 1280, or any
combination thereof, the deletion enhancing the binding capability
of the modified BoNT/B H.sub.CC targeting domain.
[0272] In another embodiment, a modified Clostridial toxin
comprising a targeting domain with enhanced binding activity
comprises a modified BoNT/C1 H.sub.CC targeting domain with
enhanced binding activity. In an aspect of this embodiment, a
modified Clostridial toxin comprises a modified BoNT/C1 H.sub.CC
targeting domain with an increased binding affinity or a modified
BoNT/C1 H.sub.CC targeting domain fragment with an increased
binding affinity. In another aspect of this embodiment, a modified
Clostridial toxin comprises a modified BoNT/C1 H.sub.CC targeting
domain with an increased binding specificity or a modified BoNT/C1
H.sub.CC targeting domain fragment with an increased binding
specificity.
[0273] In another embodiment, a modified BoNT/C1 H.sub.CC targeting
domain with enhanced binding activity comprises a modification of
amino acids 1112-1291 of SEQ ID NO: 3. In another aspect of this
embodiment, a modified BoNT/C1 H.sub.CC targeting domain with an
altered cell binding capability comprises a modified .alpha.-fold
motif of a .beta.-trefoil domain of a BoNT/C1 H.sub.CC targeting
domain, a modified .beta.-fold motif of a .beta.-trefoil domain of
a BoNT/C1 H.sub.CC targeting domain, or a modified .gamma.-fold
motif of a .beta.-trefoil domain of a BoNT/C1 H.sub.CC targeting
domain. In another aspect of this embodiment, a modified BoNT/C1
H.sub.CC targeting domain with an altered cell binding capability
comprises a modification to amino acids 1112-1150, amino acids
1167-1218, or amino acids 1230-1291 of SEQ ID NO: 3.
[0274] In other aspects of this embodiment, a modified BoNT/C1
H.sub.CC targeting domain comprising a 11-trefoil fold domain with
enhanced binding activity comprises a polypeptide having, e.g., at
least 70% amino acid identity with amino acids 1112-1150, amino
acids 1167-1218, or amino acids 1230-1291 of SEQ ID NO: 3, at least
75% amino acid identity with amino acids 1112-1150, amino acids
1167-1218, or amino acids 1230-1291 of SEQ ID NO: 3, at least 80%
amino acid identity with amino acids 1112-1150, amino acids
1167-1218, or amino acids 1230-1291 of SEQ ID NO: 3, at least 85%
amino acid identity with amino acids 1112-1150, amino acids
1167-1218, or amino acids 1230-1291 of SEQ ID NO: 3, at least 90%
amino acid identity with amino acids 1112-1150, amino acids
1167-1218, or amino acids 1230-1291 of SEQ ID NO: 3 or at least 95%
amino acid identity with amino acids 1112-1150, amino acids
1167-1218, or amino acids 1230-1291 of SEQ ID NO: 3. In yet other
aspects of this embodiment, a modified BoNT/C1 H.sub.CC targeting
domain comprising a .beta.-trefoil fold domain with enhanced
binding activity comprises a polypeptide having, e.g., at most 70%
amino acid identity with amino acids 1112-1150, amino acids
1167-1218, or amino acids 1230-1291 of SEQ ID NO: 3, at most 75%
amino acid identity with amino acids 1112-1150, amino acids
1167-1218, or amino acids 1230-1291 of SEQ ID NO: 3, at most 80%
amino acid identity with amino acids 1112-1150, amino acids
1167-1218, or amino acids 1230-1291 of SEQ ID NO: 3, at most 85%
amino acid identity with amino acids 1112-1150, amino acids
1167-1218, or amino acids 1230-1291 of SEQ ID NO: 3, at most 90%
amino acid identity with amino acids 1112-1150, amino acids
1167-1218, or amino acids 1230-1291 of SEQ ID NO: 3 or at most 95%
amino acid identity with amino acids 1112-1150, amino acids
1167-1218, or amino acids 1230-1291 of SEQ ID NO: 3.
[0275] In other aspects of this embodiment, a modified BoNT/C1
H.sub.CC targeting domain comprising a .beta.-trefoil fold domain
with enhanced binding activity comprises a polypeptide having,
e.g., at most one, two, three, four, five, six, seven, eight, nine,
10 or 20 non-contiguous amino acid substitutions relative to amino
acids 1112-1150, amino acids 1167-1218, or amino acids 1230-1291 of
SEQ ID NO: 3. In other aspects of this embodiment, a modified
BoNT/C1 H.sub.CC targeting domain with enhanced binding activity
comprises a polypeptide having, e.g., at least one, two, three,
four, five, six, seven, eight, nine, 10 or 20 non-contiguous amino
acid substitutions relative to amino acids 1112-1150, amino acids
1167-1218, or amino acids 1230-1291 of SEQ ID NO: 3. In yet other
aspects of this embodiment, a modified BoNT/C1 H.sub.CC targeting
domain comprising a .beta.-trefoil fold domain with enhanced
binding activity comprises a polypeptide having, e.g., at most one,
two, three, four, five, six, seven, eight, nine, 10 or 20
non-contiguous amino acid deletions relative to amino acids
1112-1150, amino acids 1167-1218, or amino acids 1230-1291 of SEQ
ID NO: 3. In other aspects of this embodiment, a modified BoNT/C1
H.sub.CC targeting domain comprising a .beta.-trefoil fold domain
with enhanced binding activity comprises a polypeptide having,
e.g., at least one, two, three, four, five, six, seven, eight,
nine, 10 or 20 non-contiguous amino acid deletions relative to
amino acids 1112-1150, amino acids 1167-1218, or amino acids
1230-1291 of SEQ ID NO: 3. In still other aspects of this
embodiment, a modified BoNT/C1 H.sub.CC targeting domain comprising
a .beta.-trefoil fold domain with enhanced binding activity
comprises a polypeptide having, e.g., at most one, two, three,
four, five, six, seven, eight, nine, 10 or 20 non-contiguous amino
acid additions relative to amino acids 1112-1150, amino acids
1167-1218, or amino acids 1230-1291 of SEQ ID NO: 3. In other
aspects of this embodiment, a modified BoNT/C1 H.sub.CC targeting
domain comprising a .beta.-trefoil fold domain with enhanced
binding activity comprises a polypeptide having, e.g., at least
one, two, three, four, five, six, seven, eight, nine, 10 or 20
non-contiguous amino acid additions relative to amino acids
1112-1150, amino acids 1167-1218, or amino acids 1230-1291 of SEQ
ID NO: 3.
[0276] In other aspects of this embodiment, a modified BoNT/C1
H.sub.CC targeting domain comprising a .beta.-trefoil fold domain
with enhanced binding activity comprises a polypeptide having,
e.g., at most one, two, three, four, five, six, seven, eight, nine,
10 or 20 contiguous amino acid substitutions relative to amino
acids 1112-1150, amino acids 1167-1218, or amino acids 1230-1291 of
SEQ ID NO: 3. In other aspects of this embodiment, a modified
BoNT/C1 H.sub.CC targeting domain comprising a .beta.-trefoil fold
domain with enhanced binding activity comprises a polypeptide
having, e.g., at least one, two, three, four, five, six, seven,
eight, nine, 10 or 20 contiguous amino acid substitutions relative
to amino acids 1112-1150, amino acids 1167-1218, or amino acids
1230-1291 of SEQ ID NO: 3. In yet other aspects of this embodiment,
a modified BoNT/C1 H.sub.CC targeting domain comprising a
.beta.-trefoil fold domain with enhanced binding activity comprises
a polypeptide having, e.g., at most one, two, three, four, five,
six, seven, eight, nine, 10 or 20 contiguous amino acid deletions
relative to amino acids 1112-1150, amino acids 1167-1218, or amino
acids 1230-1291 of SEQ ID NO: 3. In other aspects of this
embodiment, a modified BoNT/C1 H.sub.CC targeting domain comprising
a .beta.-trefoil fold domain with enhanced binding activity
comprises a polypeptide having, e.g., at least one, two, three,
four, five, six, seven, eight, nine, 10 or 20 contiguous amino acid
deletions relative to amino acids 1112-1150, amino acids 1167-1218,
or amino acids 1230-1291 of SEQ ID NO: 3. In still other aspects of
this embodiment, a modified BoNT/C1 H.sub.CC targeting domain
comprising a .beta.-trefoil fold domain with enhanced binding
activity comprises a polypeptide having, e.g., at most one, two,
three, four, five, six, seven, eight, nine, 10 or 20 contiguous
amino acid additions relative to amino acids 1112-1150, amino acids
1167-1218, or amino acids 1230-1291 of SEQ ID NO: 3. In other
aspects of this embodiment, a modified BoNT/C1 H.sub.CC targeting
domain comprising a .beta.-trefoil fold domain with enhanced
binding activity comprises a polypeptide having, e.g., at least
one, two, three, four, five, six, seven, eight, nine, 10 or 20
contiguous amino acid additions relative to amino acids 1112-1150,
amino acids 1167-1218, or amino acids 1230-1291 of SEQ ID NO:
3.
[0277] In another embodiment, a modified BoNT/C1 H.sub.CC targeting
domain with enhanced binding activity comprises a modified BoNT/C1
H.sub.CC targeting domain with enhanced binding activity of
comprises a modified .beta.4/.beta.5 hairpin turn of a
.beta.-trefoil domain of a BoNT/C1 H.sub.CC targeting domain or a
.beta.8/.beta.9 hairpin turn of a .beta.-trefoil domain of a
BoNT/C1 H.sub.CC targeting domain. In another aspect of this
embodiment, a modified BoNT/C1 H.sub.CC targeting domain with
enhanced binding activity comprises a modification of amino acids
1151-1166 or amino acids 1219-1229 of SEQ ID NO: 3.
[0278] In other aspects of this embodiment, a modified BoNT/C1
H.sub.CC targeting domain comprising a .beta.-trefoil fold domain
with enhanced binding activity comprises a polypeptide having,
e.g., at least 70% amino acid identity with amino acids 1151-1166
or amino acids 1219-1229 of SEQ ID NO: 3, at least 75% amino acid
identity with amino acids 1151-1166 or amino acids 1219-1229 of SEQ
ID NO: 3, at least 80% amino acid identity with amino acids
1151-1166 or amino acids 1219-1229 of SEQ ID NO: 3, at least 85%
amino acid identity with amino acids 1151-1166 or amino acids
1219-1229 of SEQ ID NO: 3, at least 90% amino acid identity with
amino acids 1151-1166 or amino acids 1219-1229 of SEQ ID NO: 3 or
at least 95% amino acid identity with amino acids 1151-1166 or
amino acids 1219-1229 of SEQ ID NO: 3. In yet other aspects of this
embodiment, a modified BoNT/C1 H.sub.CC targeting domain comprising
a .beta.-trefoil fold domain with enhanced binding activity
comprises a polypeptide having, e.g., at most 70% amino acid
identity with amino acids 1151-1166 or amino acids 1219-1229 of SEQ
ID NO: 3, at most 75% amino acid identity with amino acids
1151-1166 or amino acids 1219-1229 of SEQ ID NO: 3, at most 80%
amino acid identity with amino acids 1151-1166 or amino acids
1219-1229 of SEQ ID NO: 3, at most 85% amino acid identity with
amino acids 1151-1166 or amino acids 1219-1229 of SEQ ID NO: 3, at
most 90% amino acid identity with amino acids 1151-1166 or amino
acids 1219-1229 of SEQ ID NO: 3 or at most 95% amino acid identity
with amino acids 1151-1166 or amino acids 1219-1229 of SEQ ID NO:
3.
[0279] In other aspects of this embodiment, a modified BoNT/C1
H.sub.CC targeting domain comprising a .beta.-trefoil fold domain
with enhanced binding activity comprises a polypeptide having,
e.g., at most one, two, three, four, five, six, seven, eight, nine
or 10 non-contiguous amino acid substitutions relative to amino
acids 1151-1166 or amino acids 1219-1229 of SEQ ID NO: 3. In other
aspects of this embodiment, a modified BoNT/C1 H.sub.CC targeting
domain comprises a polypeptide having, e.g., at least one, two,
three, four, five, six, seven, eight, nine or 10 non-contiguous
amino acid substitutions relative to amino acids 1151-1166 or amino
acids 1219-1229 of SEQ ID NO: 3. In other aspects of this
embodiment, a non-contiguous amino acid substitution of any amino
acid from amino acids 1151-1166 or amino acids 1219-1229 of SEQ ID
NO: 3 can be replaced with glycine. In other aspects of this
embodiment, a non-contiguous amino acid substitution of any
hydrophobic amino acid from amino acids 1151-1166 or amino acids
1219-1229 of SEQ ID NO: 3 can be replaced with phenylalanine. In
yet other aspects of this embodiment, a modified BoNT/C1 H.sub.CC
targeting domain comprising a .beta.-trefoil fold domain with
enhanced binding activity comprises a polypeptide having, e.g., at
most one, two, three, four, five, six, seven, eight, nine or 10
non-contiguous amino acid deletions relative to amino acids
1151-1166 or amino acids 1219-1229 of SEQ ID NO: 3. In other
aspects of this embodiment, a modified BoNT/C1 H.sub.CC targeting
domain comprising a .beta.-trefoil fold domain with enhanced
binding activity comprises a polypeptide having, e.g., at least
one, two, three, four, five, six, seven, eight, nine or 10
non-contiguous amino acid deletions relative to amino acids
1151-1166 or amino acids 1219-1229 of SEQ ID NO: 3. In still other
aspects of this embodiment, a modified BoNT/C1 H.sub.CC targeting
domain comprising a .beta.-trefoil fold domain with enhanced
binding activity comprises a polypeptide having, e.g., at most one,
two, three, four, five, six, seven, eight, nine or 10
non-contiguous amino acid additions relative to amino acids
1151-1166 or amino acids 1219-1229 of SEQ ID NO: 3. In other
aspects of this embodiment, a modified BoNT/C1 H.sub.CC targeting
domain comprising a .beta.-trefoil fold domain with enhanced
binding activity comprises a polypeptide having, e.g., at least
one, two, three, four, five, six, seven, eight, nine or 10
non-contiguous amino acid additions relative to amino acids
1151-1166 or amino acids 1219-1229 of SEQ ID NO: 3.
[0280] In other aspects of this embodiment, a modified BoNT/C1
H.sub.CC targeting domain comprising a .beta.-trefoil fold domain
with enhanced binding activity comprises a polypeptide having,
e.g., at most one, two, three, four, five, six, seven, eight, nine
or 10 contiguous amino acid substitutions relative to amino acids
1151-1166 or amino acids 1219-1229 of SEQ ID NO: 3. In other
aspects of this embodiment, a modified BoNT/C1 H.sub.CC targeting
domain comprising a .beta.-trefoil fold domain with enhanced
binding activity comprises a polypeptide having, e.g., at least
one, two, three, four, five, six, seven, eight, nine or 10
contiguous amino acid substitutions relative to amino acids
1151-1166 or amino acids 1219-1229 of SEQ ID NO: 3. In other
aspects of this embodiment, contiguous amino acid substitutions of
amino acids from amino acids 1151-1166 or amino acids 1219-1229 of
SEQ ID NO: 3 can be replaced with glycine. In other aspects of this
embodiment, contiguous amino acid substitutions of hydrophobic
amino acids from amino acids 1151-1166 or amino acids 1219-1229 of
SEQ ID NO: 3 can be replaced with phenylalanine. In yet other
aspects of this embodiment, a modified BoNT/C1 H.sub.CC targeting
domain comprising a .beta.-trefoil fold domain with enhanced
binding activity comprises a polypeptide having, e.g., at most one,
two, three, four, five, six, seven, eight, nine or 10 contiguous
amino acid deletions relative to amino acids 1151-1166 or amino
acids 1219-1229 of SEQ ID NO: 3. In other aspects of this
embodiment, a modified BoNT/C1H.sub.CC targeting domain comprising
a .beta.-trefoil fold domain with enhanced binding activity
comprises a polypeptide having, e.g., at least one, two, three,
four, five, six, seven, eight, nine or 10 contiguous amino acid
deletions relative to amino acids 1151-1166 or amino acids
1219-1229 of SEQ ID NO: 3. In still other aspects of this
embodiment, a modified BoNT/C1 H.sub.CC targeting domain comprising
a .beta.-trefoil fold domain with enhanced binding activity
comprises a polypeptide having, e.g., at most one, two, three,
four, five, six, seven, eight, nine or 10 contiguous amino acid
additions relative to amino acids 1151-1166 or amino acids
1219-1229 of SEQ ID NO: 3. In other aspects of this embodiment, a
modified BoNT/C1 H.sub.CC targeting domain comprising a
.beta.-trefoil fold domain with enhanced binding activity comprises
a polypeptide having, e.g., at least one, two, three, four, five,
six, seven, eight, nine or 10 contiguous amino acid additions
relative to amino acids 1151-1166 or amino acids 1219-1229 of SEQ
ID NO: 3.
[0281] In other aspects of this embodiment, a modified BoNT/C1
H.sub.CC targeting domain with enhanced binding activity comprises
a substitution of amino acid Trp 1102, Gly 1103, Leu 1106, Tyr
1112, Tyr 1113, Gly 1145, Ile 1150, Asp 1166, Glu 1196, Ile 1247,
Gly 1256, Trp 1258, Tyr 1259, H is 1261, Gly 1281 or Trp 1284, or
any combination thereof, the substitution enhancing the binding
activity of the modified BoNT/C1 H.sub.CC targeting domain. In
other aspects of this embodiment, a modified BoNT/C1 H.sub.CC
targeting domain with enhanced binding activity comprises a
deletion of amino acid Trp 1102, Gly 1103, Leu 1106, Tyr 1112, Tyr
1113, Gly 1145, Ile 1150, Asp 1166, Glu 1196, Ile 1247, Gly 1256,
Trp 1258, Tyr 1259, H is 1261, Gly 1281 or Trp 1284, or any
combination thereof, the deletion enhancing the binding activity of
the modified BoNT/C1 H.sub.CC targeting domain.
[0282] In another embodiment, a modified Clostridial toxin
comprising a targeting domain with enhanced binding activity
comprises a modified BoNT/D H.sub.CC targeting domain with enhanced
binding activity. In an aspect of this embodiment, a modified
Clostridial toxin comprises a modified BoNT/D H.sub.CC targeting
domain with an increased binding affinity or a modified BoNT/D
H.sub.CC targeting domain fragment with an increased binding
affinity. In another aspect of this embodiment, a modified
Clostridial toxin comprises a modified BoNT/D H.sub.CC targeting
domain with an increased binding specificity or a modified BoNT/D
H.sub.CC targeting domain fragment with an increased binding
specificity.
[0283] In another embodiment, a modified BoNT/D H.sub.CC targeting
domain with enhanced binding activity comprises a modification of
amino acids 1099-1276 of SEQ ID NO: 4. In another aspect of this
embodiment, a modified BoNT/D H.sub.CC targeting domain with an
altered cell binding capability comprises a modified .alpha.-fold
motif of a .beta.-trefoil domain of a BoNT/D H.sub.CC targeting
domain, a modified .beta.-fold motif of a .beta.-trefoil domain of
a BoNT/D H.sub.CC targeting domain, or a modified .gamma.-fold
motif of a .beta.-trefoil domain of a BoNT/D H.sub.CC targeting
domain. In another aspect of this embodiment, a modified BoNT/D
H.sub.CC targeting domain with an altered cell binding capability
comprises a modification to amino acids 1099-1137, amino acids
1154-1207, or amino acids 1219-1276 of SEQ ID NO: 4.
[0284] In other aspects of this embodiment, a modified BoNT/D
H.sub.CC targeting domain comprising a .beta.-trefoil fold domain
with enhanced binding activity comprises a polypeptide having,
e.g., at least 70% amino acid identity with amino acids 1099-1137,
amino acids 1154-1207, or amino acids 1219-1276 of SEQ ID NO: 4, at
least 75% amino acid identity with amino acids 1099-1137, amino
acids 1154-1207, or amino acids 1219-1276 of SEQ ID NO: 4, at least
80% amino acid identity with amino acids 1099-1137, amino acids
1154-1207, or amino acids 1219-1276 of SEQ ID NO: 4, at least 85%
amino acid identity with amino acids 1099-1137, amino acids
1154-1207, or amino acids 1219-1276 of SEQ ID NO: 4, at least 90%
amino acid identity with amino acids 1099-1137, amino acids
1154-1207, or amino acids 1219-1276 of SEQ ID NO: 4 or at least 95%
amino acid identity with amino acids 1099-1137, amino acids
1154-1207, or amino acids 1219-1276 of SEQ ID NO: 4. In yet other
aspects of this embodiment, a modified BoNT/D H.sub.CC targeting
domain comprising a .beta.-trefoil fold domain with enhanced
binding activity comprises a polypeptide having, e.g., at most 70%
amino acid identity with amino acids 1099-1137, amino acids
1154-1207, or amino acids 1219-1276 of SEQ ID NO: 4, at most 75%
amino acid identity with amino acids 1099-1137, amino acids
1154-1207, or amino acids 1219-1276 of SEQ ID NO: 4, at most 80%
amino acid identity with amino acids 1099-1137, amino acids
1154-1207, or amino acids 1219-1276 of SEQ ID NO: 4, at most 85%
amino acid identity with amino acids 1099-1137, amino acids
1154-1207, or amino acids 1219-1276 of SEQ ID NO: 4, at most 90%
amino acid identity with amino acids 1099-1137, amino acids
1154-1207, or amino acids 1219-1276 of SEQ ID NO: 4 or at most 95%
amino acid identity with amino acids 1099-1137, amino acids
1154-1207, or amino acids 1219-1276 of SEQ ID NO: 4.
[0285] In other aspects of this embodiment, a modified BoNT/D
H.sub.CC targeting domain comprising a .beta.-trefoil fold domain
with enhanced binding activity comprises a polypeptide having,
e.g., at most one, two, three, four, five, six, seven, eight, nine,
10 or 20 non-contiguous amino acid substitutions relative to amino
acids 1099-1137, amino acids 1154-1207, or amino acids 1219-1276 of
SEQ ID NO: 4. In other aspects of this embodiment, a modified
BoNT/D H.sub.CC targeting domain with enhanced binding activity
comprises a polypeptide having, e.g., at least one, two, three,
four, five, six, seven, eight, nine, 10 or 20 non-contiguous amino
acid substitutions relative to amino acids 1099-1137, amino acids
1154-1207, or amino acids 1219-1276 of SEQ ID NO: 4. In yet other
aspects of this embodiment, a modified BoNT/D H.sub.CC targeting
domain comprising a .beta.-trefoil fold domain with enhanced
binding activity comprises a polypeptide having, e.g., at most one,
two, three, four, five, six, seven, eight, nine, 10 or 20
non-contiguous amino acid deletions relative to amino acids
1099-1137, amino acids 1154-1207, or amino acids 1219-1276 of SEQ
ID NO: 4. In other aspects of this embodiment, a modified BoNT/D
H.sub.CC targeting domain comprising a .beta.-trefoil fold domain
with enhanced binding activity comprises a polypeptide having,
e.g., at least one, two, three, four, five, six, seven, eight,
nine, 10 or 20 non-contiguous amino acid deletions relative to
amino acids 1099-1137, amino acids 1154-1207, or amino acids
1219-1276 of SEQ ID NO: 4. In still other aspects of this
embodiment, a modified BoNT/D H.sub.CC targeting domain comprising
a .beta.-trefoil fold domain with enhanced binding activity
comprises a polypeptide having, e.g., at most one, two, three,
four, five, six, seven, eight, nine, 10 or 20 non-contiguous amino
acid additions relative to amino acids 1099-1137, amino acids
1154-1207, or amino acids 1219-1276 of SEQ ID NO: 4. In other
aspects of this embodiment, a modified BoNT/D H.sub.CC targeting
domain comprising a .beta.-trefoil fold domain with enhanced
binding activity comprises a polypeptide having, e.g., at least
one, two, three, four, five, six, seven, eight, nine, 10 or 20
non-contiguous amino acid additions relative to amino acids
1099-1137, amino acids 1154-1207, or amino acids 1219-1276 of SEQ
ID NO: 4.
[0286] In other aspects of this embodiment, a modified BoNT/D
H.sub.CC targeting domain comprising a .beta.-trefoil fold domain
with enhanced binding activity comprises a polypeptide having,
e.g., at most one, two, three, four, five, six, seven, eight, nine,
10 or 20 contiguous amino acid substitutions relative to amino
acids 1099-1137, amino acids 1154-1207, or amino acids 1219-1276 of
SEQ ID NO: 4. In other aspects of this embodiment, a modified
BoNT/D H.sub.CC targeting domain comprising a .beta.-trefoil fold
domain with enhanced binding activity comprises a polypeptide
having, e.g., at least one, two, three, four, five, six, seven,
eight, nine, 10 or 20 contiguous amino acid substitutions relative
to amino acids 1099-1137, amino acids 1154-1207, or amino acids
1219-1276 of SEQ ID NO: 4. In yet other aspects of this embodiment,
a modified BoNT/D H.sub.CC targeting domain comprising a
.beta.-trefoil fold domain with enhanced binding activity comprises
a polypeptide having, e.g., at most one, two, three, four, five,
six, seven, eight, nine, 10 or 20 contiguous amino acid deletions
relative to amino acids 1099-1137, amino acids 1154-1207, or amino
acids 1219-1276 of SEQ ID NO: 4. In other aspects of this
embodiment, a modified BoNT/D H.sub.CC targeting domain comprising
a .beta.-trefoil fold domain with enhanced binding activity
comprises a polypeptide having, e.g., at least one, two, three,
four, five, six, seven, eight, nine, 10 or 20 contiguous amino acid
deletions relative to amino acids 1099-1137, amino acids 1154-1207,
or amino acids 1219-1276 of SEQ ID NO: 4. In still other aspects of
this embodiment, a modified BoNT/D H.sub.CC targeting domain
comprising a .beta.-trefoil fold domain with enhanced binding
activity comprises a polypeptide having, e.g., at most one, two,
three, four, five, six, seven, eight, nine, 10 or 20 contiguous
amino acid additions relative to amino acids 1099-1137, amino acids
1154-1207, or amino acids 1219-1276 of SEQ ID NO: 4. In other
aspects of this embodiment, a modified BoNT/D H.sub.CC targeting
domain comprising a .beta.-trefoil fold domain with enhanced
binding activity comprises a polypeptide having, e.g., at least
one, two, three, four, five, six, seven, eight, nine, 10 or 20
contiguous amino acid additions relative to amino acids 1099-1137,
amino acids 1154-1207, or amino acids 1219-1276 of SEQ ID NO:
4.
[0287] In another embodiment, a modified BoNT/D H.sub.CC targeting
domain with enhanced binding activity comprises a modified BoNT/D
H.sub.CC targeting domain with enhanced binding activity of
comprises a modified .beta.4/.beta.5 hairpin turn of a
.beta.-trefoil domain of a BoNT/D H.sub.CC targeting domain or a
.beta.8/.beta.9 hairpin turn of a .beta.-trefoil domain of a BoNT/D
H.sub.CC targeting domain. In another aspect of this embodiment, a
modified BoNT/D H.sub.CC targeting domain with enhanced binding
activity comprises a modification of amino acids 1138-1153 or amino
acids 1208-1218 of SEQ ID NO: 4.
[0288] In other aspects of this embodiment, a modified BoNT/D
H.sub.CC targeting domain comprising a .beta.-trefoil fold domain
with enhanced binding activity comprises a polypeptide having,
e.g., at least 70% amino acid identity with amino acids 1138-1153
or amino acids 1208-1218 of SEQ ID NO: 4, at least 75% amino acid
identity with amino acids 1138-1153 or amino acids 1208-1218 of SEQ
ID NO: 4, at least 80% amino acid identity with amino acids
1138-1153 or amino acids 1208-1218 of SEQ ID NO: 4, at least 85%
amino acid identity with amino acids 1138-1153 or amino acids
1208-1218 of SEQ ID NO: 4, at least 90% amino acid identity with
amino acids 1138-1153 or amino acids 1208-1218 of SEQ ID NO: 4 or
at least 95% amino acid identity with amino acids 1138-1153 or
amino acids 1208-1218 of SEQ ID NO: 4. In yet other aspects of this
embodiment, a modified BoNT/D H.sub.CC targeting domain comprising
a .beta.-trefoil fold domain with enhanced binding activity
comprises a polypeptide having, e.g., at most 70% amino acid
identity with amino acids 1138-1153 or amino acids 1208-1218 of SEQ
ID NO: 4, at most 75% amino acid identity with amino acids
1138-1153 or amino acids 1208-1218 of SEQ ID NO: 4, at most 80%
amino acid identity with amino acids 1138-1153 or amino acids
1208-1218 of SEQ ID NO: 4, at most 85% amino acid identity with
amino acids 1138-1153 or amino acids 1208-1218 of SEQ ID NO: 4, at
most 90% amino acid identity with amino acids 1138-1153 or amino
acids 1208-1218 of SEQ ID NO: 4 or at most 95% amino acid identity
with amino acids 1138-1153 or amino acids 1208-1218 of SEQ ID NO:
4.
[0289] In other aspects of this embodiment, a modified BoNT/D
H.sub.CC targeting domain comprising a .beta.-trefoil fold domain
with enhanced binding activity comprises a polypeptide having,
e.g., at most one, two, three, four, five, six, seven, eight, nine
or 10 non-contiguous amino acid substitutions relative to amino
acids 1138-1153 or amino acids 1208-1218 of SEQ ID NO: 4. In other
aspects of this embodiment, a modified BoNT/D H.sub.CC targeting
domain comprises a polypeptide having, e.g., at least one, two,
three, four, five, six, seven, eight, nine or 10 non-contiguous
amino acid substitutions relative to amino acids 1138-1153 or amino
acids 1208-1218 of SEQ ID NO: 4. In other aspects of this
embodiment, a non-contiguous amino acid substitution of any amino
acid from amino acids 1138-1153 or amino acids 1208-1218 of SEQ ID
NO: 4 can be replaced with glycine. In other aspects of this
embodiment, a non-contiguous amino acid substitution of any
hydrophobic amino acid from amino acids 1138-1153 or amino acids
1208-1218 of SEQ ID NO: 4 can be replaced with phenylalanine. In
yet other aspects of this embodiment, a modified BoNT/D H.sub.CC
targeting domain comprising a .beta.-trefoil fold domain with
enhanced binding activity comprises a polypeptide having, e.g., at
most one, two, three, four, five, six, seven, eight, nine or 10
non-contiguous amino acid deletions relative to amino acids
1138-1153 or amino acids 1208-1218 of SEQ ID NO: 4. In other
aspects of this embodiment, a modified BoNT/D H.sub.CC targeting
domain comprising a .beta.-trefoil fold domain with enhanced
binding activity comprises a polypeptide having, e.g., at least
one, two, three, four, five, six, seven, eight, nine or 10
non-contiguous amino acid deletions relative to amino acids
1138-1153 or amino acids 1208-1218 of SEQ ID NO: 4. In still other
aspects of this embodiment, a modified BoNT/D H.sub.CC targeting
domain comprising a .beta.-trefoil fold domain with enhanced
binding activity comprises a polypeptide having, e.g., at most one,
two, three, four, five, six, seven, eight, nine or 10
non-contiguous amino acid additions relative to amino acids
1138-1153 or amino acids 1208-1218 of SEQ ID NO: 4. In other
aspects of this embodiment, a modified BoNT/D H.sub.CC targeting
domain comprising a .beta.-trefoil fold domain with enhanced
binding activity comprises a polypeptide having, e.g., at least
one, two, three, four, five, six, seven, eight, nine or 10
non-contiguous amino acid additions relative to amino acids
1138-1153 or amino acids 1208-1218 of SEQ ID NO: 4.
[0290] In other aspects of this embodiment, a modified BoNT/D
H.sub.CC targeting domain comprising a .beta.-trefoil fold domain
with enhanced binding activity comprises a polypeptide having,
e.g., at most one, two, three, four, five, six, seven, eight, nine
or 10 contiguous amino acid substitutions relative to amino acids
1138-1153 or amino acids 1208-1218 of SEQ ID NO: 4. In other
aspects of this embodiment, a modified BoNT/D H.sub.CC targeting
domain comprising a .beta.-trefoil fold domain with enhanced
binding activity comprises a polypeptide having, e.g., at least
one, two, three, four, five, six, seven, eight, nine or 10
contiguous amino acid substitutions relative to amino acids
1138-1153 or amino acids 1208-1218 of SEQ ID NO: 4. In other
aspects of this embodiment, contiguous amino acid substitutions of
amino acids from amino acids 1138-1153 or amino acids 1208-1218 of
SEQ ID NO: 4 can be replaced with glycine. In other aspects of this
embodiment, contiguous amino acid substitutions of hydrophobic
amino acids from amino acids 1138-1153 or amino acids 1208-1218 of
SEQ ID NO: 4 can be replaced with phenylalanine. In yet other
aspects of this embodiment, a modified BoNT/D H.sub.CC targeting
domain comprising a .beta.-trefoil fold domain with enhanced
binding activity comprises a polypeptide having, e.g., at most one,
two, three, four, five, six, seven, eight, nine or 10 contiguous
amino acid deletions relative to amino acids 1138-1153 or amino
acids 1208-1218 of SEQ ID NO: 4. In other aspects of this
embodiment, a modified BoNT/D H.sub.CC targeting domain comprising
a .beta.-trefoil fold domain with enhanced binding activity
comprises a polypeptide having, e.g., at least one, two, three,
four, five, six, seven, eight, nine or 10 contiguous amino acid
deletions relative to amino acids 1138-1153 or amino acids
1208-1218 of SEQ ID NO: 4. In still other aspects of this
embodiment, a modified BoNT/D H.sub.CC targeting domain comprising
a .beta.-trefoil fold domain with enhanced binding activity
comprises a polypeptide having, e.g., at most one, two, three,
four, five, six, seven, eight, nine or 10 contiguous amino acid
additions relative to amino acids 1138-1153 or amino acids
1208-1218 of SEQ ID NO: 4. In other aspects of this embodiment, a
modified BoNT/D H.sub.CC targeting domain comprising a
.beta.-trefoil fold domain with enhanced binding activity comprises
a polypeptide having, e.g., at least one, two, three, four, five,
six, seven, eight, nine or 10 contiguous amino acid additions
relative to amino acids 1138-1153 or amino acids 1208-1218 of SEQ
ID NO: 4.
[0291] In other aspects of this embodiment, a modified BoNT/D
H.sub.CC targeting domain with enhanced binding activity comprises
a substitution of amino acid Trp 1089, Gly 1090, Leu 1093, Tyr
1099, Tyr 1100, Gly 1132, Ile 1137, Asp 1153, Asn 1186, Lys 1236,
Trp 1238, Arg 1239, Phe 1242, Ser 1262 or Trp 1265, or any
combination thereof, the substitution enhancing the binding
activity of the modified BoNT/D H.sub.CC targeting domain. In other
aspects of this embodiment, a modified BoNT/D H.sub.CC targeting
domain with enhanced binding activity comprises a deletion of amino
acid Trp 1089, Gly 1090, Leu 1093, Tyr 1099, Tyr 1100, Gly 1132,
Ile 1137, Asp 1153, Asn 1186, Lys 1236, Trp 1238, Arg 1239, Phe
1242, Ser 1262 or Trp 1265, or any combination thereof, the
deletion enhancing the binding activity of the modified BoNT/D
H.sub.CC targeting domain.
[0292] In another embodiment, a modified Clostridial toxin
comprising a targeting domain with enhanced binding activity
comprises a modified BoNT/E H.sub.CC targeting domain with enhanced
binding activity. In an aspect of this embodiment, a modified
Clostridial toxin comprises a modified BoNT/E H.sub.CC targeting
domain with an increased binding affinity or a modified BoNT/E
H.sub.CC targeting domain fragment with an increased binding
affinity. In another aspect of this embodiment, a modified
Clostridial toxin comprises a modified BoNT/E H.sub.CC targeting
domain with an increased binding specificity or a modified BoNT/E
H.sub.CC targeting domain fragment with an increased binding
specificity.
[0293] In another embodiment, a modified BoNT/E H.sub.CC targeting
domain with enhanced binding activity comprises a modification of
amino acids 1086-1252 of SEQ ID NO: 5. In another aspect of this
embodiment, a modified BoNT/E H.sub.CC targeting domain with an
altered cell binding capability comprises a modified .alpha.-fold
motif of a .beta.-trefoil domain of a BoNT/E H.sub.CC targeting
domain, a modified .beta.-fold motif of a .beta.-trefoil domain of
a BoNT/E H.sub.CC targeting domain, or a modified .gamma.-fold
motif of a .beta.-trefoil domain of a BoNT/E H.sub.CC targeting
domain. In another aspect of this embodiment, a modified BoNT/E
H.sub.CC targeting domain with an altered cell binding capability
comprises a modification to amino acids 1086-1129, amino acids
1147-1190, or amino acids 1199-1252 of SEQ ID NO: 5.
[0294] In other aspects of this embodiment, a modified BoNT/E
H.sub.CC targeting domain comprising a 11-trefoil fold domain with
enhanced binding activity comprises a polypeptide having, e.g., at
least 70% amino acid identity with amino acids 1086-1129, amino
acids 1147-1190, or amino acids 1199-1252 of SEQ ID NO: 5, at least
75% amino acid identity with amino acids 1086-1129, amino acids
1147-1190, or amino acids 1199-1252 of SEQ ID NO: 5, at least 80%
amino acid identity with amino acids 1086-1129, amino acids
1147-1190, or amino acids 1199-1252 of SEQ ID NO: 5, at least 85%
amino acid identity with amino acids 1086-1129, amino acids
1147-1190, or amino acids 1199-1252 of SEQ ID NO: 5, at least 90%
amino acid identity with amino acids 1086-1129, amino acids
1147-1190, or amino acids 1199-1252 of SEQ ID NO: 5 or at least 95%
amino acid identity with amino acids 1086-1129, amino acids
1147-1190, or amino acids 1199-1252 of SEQ ID NO: 5. In yet other
aspects of this embodiment, a modified BoNT/E H.sub.CC targeting
domain comprising a .beta.-trefoil fold domain with enhanced
binding activity comprises a polypeptide having, e.g., at most 70%
amino acid identity with amino acids 1086-1129, amino acids
1147-1190, or amino acids 1199-1252 of SEQ ID NO: 5, at most 75%
amino acid identity with amino acids 1086-1129, amino acids
1147-1190, or amino acids 1199-1252 of SEQ ID NO: 5, at most 80%
amino acid identity with amino acids 1086-1129, amino acids
1147-1190, or amino acids 1199-1252 of SEQ ID NO: 5, at most 85%
amino acid identity with amino acids 1086-1129, amino acids
1147-1190, or amino acids 1199-1252 of SEQ ID NO: 5, at most 90%
amino acid identity with amino acids 1086-1129, amino acids
1147-1190, or amino acids 1199-1252 of SEQ ID NO: 5 or at most 95%
amino acid identity with amino acids 1086-1129, amino acids
1147-1190, or amino acids 1199-1252 of SEQ ID NO: 5.
[0295] In other aspects of this embodiment, a modified BoNT/E
H.sub.CC targeting domain comprising a .beta.-trefoil fold domain
with enhanced binding activity comprises a polypeptide having,
e.g., at most one, two, three, four, five, six, seven, eight, nine,
10 or 20 non-contiguous amino acid substitutions relative to amino
acids 1086-1129, amino acids 1147-1190, or amino acids 1199-1252 of
SEQ ID NO: 5. In other aspects of this embodiment, a modified
BoNT/E H.sub.CC targeting domain with enhanced binding activity
comprises a polypeptide having, e.g., at least one, two, three,
four, five, six, seven, eight, nine, 10 or 20 non-contiguous amino
acid substitutions relative to amino acids 1086-1129, amino acids
1147-1190, or amino acids 1199-1252 of SEQ ID NO: 5. In yet other
aspects of this embodiment, a modified BoNT/E H.sub.CC targeting
domain comprising a .beta.-trefoil fold domain with enhanced
binding activity comprises a polypeptide having, e.g., at most one,
two, three, four, five, six, seven, eight, nine, 10 or 20
non-contiguous amino acid deletions relative to amino acids
1086-1129, amino acids 1147-1190, or amino acids 1199-1252 of SEQ
ID NO: 5. In other aspects of this embodiment, a modified BoNT/E
H.sub.CC targeting domain comprising a .beta.-trefoil fold domain
with enhanced binding activity comprises a polypeptide having,
e.g., at least one, two, three, four, five, six, seven, eight,
nine, 10 or 20 non-contiguous amino acid deletions relative to
amino acids 1086-1129, amino acids 1147-1190, or amino acids
1199-1252 of SEQ ID NO: 5. In still other aspects of this
embodiment, a modified BoNT/E H.sub.CC targeting domain comprising
a .beta.-trefoil fold domain with enhanced binding activity
comprises a polypeptide having, e.g., at most one, two, three,
four, five, six, seven, eight, nine, 10 or 20 non-contiguous amino
acid additions relative to amino acids 1086-1129, amino acids
1147-1190, or amino acids 1199-1252 of SEQ ID NO: 5. In other
aspects of this embodiment, a modified BoNT/E H.sub.CC targeting
domain comprising a .beta.-trefoil fold domain with enhanced
binding activity comprises a polypeptide having, e.g., at least
one, two, three, four, five, six, seven, eight, nine, 10 or 20
non-contiguous amino acid additions relative to amino acids
1086-1129, amino acids 1147-1190, or amino acids 1199-1252 of SEQ
ID NO: 5.
[0296] In other aspects of this embodiment, a modified BoNT/E
H.sub.CC targeting domain comprising a .beta.-trefoil fold domain
with enhanced binding activity comprises a polypeptide having,
e.g., at most one, two, three, four, five, six, seven, eight, nine,
10 or 20 contiguous amino acid substitutions relative to amino
acids 1086-1129, amino acids 1147-1190, or amino acids 1199-1252 of
SEQ ID NO: 5. In other aspects of this embodiment, a modified
BoNT/E H.sub.CC targeting domain comprising a .beta.-trefoil fold
domain with enhanced binding activity comprises a polypeptide
having, e.g., at least one, two, three, four, five, six, seven,
eight, nine, 10 or 20 contiguous amino acid substitutions relative
to amino acids 1086-1129, amino acids 1147-1190, or amino acids
1199-1252 of SEQ ID NO: 5. In yet other aspects of this embodiment,
a modified BoNT/E H.sub.CC targeting domain comprising a
.beta.-trefoil fold domain with enhanced binding activity comprises
a polypeptide having, e.g., at most one, two, three, four, five,
six, seven, eight, nine, 10 or 20 contiguous amino acid deletions
relative to amino acids 1086-1129, amino acids 1147-1190, or amino
acids 1199-1252 of SEQ ID NO: 5. In other aspects of this
embodiment, a modified BoNT/E H.sub.CC targeting domain comprising
a .beta.-trefoil fold domain with enhanced binding activity
comprises a polypeptide having, e.g., at least one, two, three,
four, five, six, seven, eight, nine, 10 or 20 contiguous amino acid
deletions relative to amino acids 1086-1129, amino acids 1147-1190,
or amino acids 1199-1252 of SEQ ID NO: 5. In still other aspects of
this embodiment, a modified BoNT/E H.sub.CC targeting domain
comprising a .beta.-trefoil fold domain with enhanced binding
activity comprises a polypeptide having, e.g., at most one, two,
three, four, five, six, seven, eight, nine, 10 or 20 contiguous
amino acid additions relative to amino acids 1086-1129, amino acids
1147-1190, or amino acids 1199-1252 of SEQ ID NO: 5. In other
aspects of this embodiment, a modified BoNT/E H.sub.CC targeting
domain comprising a .beta.-trefoil fold domain with enhanced
binding activity comprises a polypeptide having, e.g., at least
one, two, three, four, five, six, seven, eight, nine, 10 or 20
contiguous amino acid additions relative to amino acids 1086-1129,
amino acids 1147-1190, or amino acids 1199-1252 of SEQ ID NO:
5.
[0297] In another embodiment, a modified BoNT/E H.sub.CC targeting
domain with enhanced binding activity comprises a modified BoNT/E
H.sub.CC targeting domain with enhanced binding activity of
comprises a modified .beta.4/.beta.5 hairpin turn of a
.beta.-trefoil domain of a BoNT/E H.sub.CC targeting domain or a
.beta.8/.beta.9 hairpin turn of a .beta.-trefoil domain of a BoNT/E
H.sub.CC targeting domain. In another aspect of this embodiment, a
modified BoNT/E H.sub.CC targeting domain with enhanced binding
activity comprises a modification of amino acids 1130-1146 or amino
acids 1191-1198 of SEQ ID NO: 5.
[0298] In other aspects of this embodiment, a modified BoNT/E
H.sub.CC targeting domain comprising a 11-trefoil fold domain with
enhanced binding activity comprises a polypeptide having, e.g., at
least 70% amino acid identity with amino acids 1130-1146 or amino
acids 1191-1198 of SEQ ID NO: 5, at least 75% amino acid identity
with amino acids 1130-1146 or amino acids 1191-1198 of SEQ ID NO:
5, at least 80% amino acid identity with amino acids 1130-1146 or
amino acids 1191-1198 of SEQ ID NO: 5, at least 85% amino acid
identity with amino acids 1130-1146 or amino acids 1191-1198 of SEQ
ID NO: 5, at least 90% amino acid identity with amino acids
1130-1146 or amino acids 1191-1198 of SEQ ID NO: 5 or at least 95%
amino acid identity with amino acids 1130-1146 or amino acids
1191-1198 of SEQ ID NO: 5. In yet other aspects of this embodiment,
a modified BoNT/E H.sub.CC targeting domain comprising a
.beta.-trefoil fold domain with enhanced binding activity comprises
a polypeptide having, e.g., at most 70% amino acid identity with
amino acids 1130-1146 or amino acids 1191-1198 of SEQ ID NO: 5, at
most 75% amino acid identity with amino acids 1130-1146 or amino
acids 1191-1198 of SEQ ID NO: 5, at most 80% amino acid identity
with amino acids 1130-1146 or amino acids 1191-1198 of SEQ ID NO:
5, at most 85% amino acid identity with amino acids 1130-1146 or
amino acids 1191-1198 of SEQ ID NO: 5, at most 90% amino acid
identity with amino acids 1130-1146 or amino acids 1191-1198 of SEQ
ID NO: 5 or at most 95% amino acid identity with amino acids
1130-1146 or amino acids 1191-1198 of SEQ ID NO: 5.
[0299] In other aspects of this embodiment, a modified BoNT/E
H.sub.CC targeting domain comprising a .beta.-trefoil fold domain
with enhanced binding activity comprises a polypeptide having,
e.g., at most one, two, three, four, five, six, seven, eight, nine
or 10 non-contiguous amino acid substitutions relative to amino
acids 1130-1146 or amino acids 1191-1198 of SEQ ID NO: 5. In other
aspects of this embodiment, a modified BoNT/E H.sub.CC targeting
domain comprises a polypeptide having, e.g., at least one, two,
three, four, five, six, seven, eight, nine or 10 non-contiguous
amino acid substitutions relative to amino acids 1130-1146 or amino
acids 1191-1198 of SEQ ID NO: 5. In other aspects of this
embodiment, a non-contiguous amino acid substitution of any amino
acid from amino acids 1130-1146 or amino acids 1191-1198 of SEQ ID
NO: 5 can be replaced with glycine. In other aspects of this
embodiment, a non-contiguous amino acid substitution of any
hydrophobic amino acid from amino acids 1130-1146 or amino acids
1191-1198 of SEQ ID NO: 5 can be replaced with phenylalanine. In
yet other aspects of this embodiment, a modified BoNT/E H.sub.CC
targeting domain comprising a .beta.-trefoil fold domain with
enhanced binding activity comprises a polypeptide having, e.g., at
most one, two, three, four, five, six, seven, eight, nine or 10
non-contiguous amino acid deletions relative to amino acids
1130-1146 or amino acids 1191-1198 of SEQ ID NO: 5. In other
aspects of this embodiment, a modified BoNT/E H.sub.CC targeting
domain comprising a .beta.-trefoil fold domain with enhanced
binding activity comprises a polypeptide having, e.g., at least
one, two, three, four, five, six, seven, eight, nine or 10
non-contiguous amino acid deletions relative to amino acids
1130-1146 or amino acids 1191-1198 of SEQ ID NO: 5. In still other
aspects of this embodiment, a modified BoNT/E H.sub.CC targeting
domain comprising a .beta.-trefoil fold domain with enhanced
binding activity comprises a polypeptide having, e.g., at most one,
two, three, four, five, six, seven, eight, nine or 10
non-contiguous amino acid additions relative to amino acids
1130-1146 or amino acids 1191-1198 of SEQ ID NO: 5. In other
aspects of this embodiment, a modified BoNT/E H.sub.CC targeting
domain comprising a .beta.-trefoil fold domain with enhanced
binding activity comprises a polypeptide having, e.g., at least
one, two, three, four, five, six, seven, eight, nine or 10
non-contiguous amino acid additions relative to amino acids
1130-1146 or amino acids 1191-1198 of SEQ ID NO: 5.
[0300] In other aspects of this embodiment, a modified BoNT/E
H.sub.CC targeting domain comprising a .beta.-trefoil fold domain
with enhanced binding activity comprises a polypeptide having,
e.g., at most one, two, three, four, five, six, seven, eight, nine
or 10 contiguous amino acid substitutions relative to amino acids
1130-1146 or amino acids 1191-1198 of SEQ ID NO: 5. In other
aspects of this embodiment, a modified BoNT/E H.sub.CC targeting
domain comprising a .beta.-trefoil fold domain with enhanced
binding activity comprises a polypeptide having, e.g., at least
one, two, three, four, five, six, seven, eight, nine or 10
contiguous amino acid substitutions relative to amino acids
1130-1146 or amino acids 1191-1198 of SEQ ID NO: 5. In other
aspects of this embodiment, contiguous amino acid substitutions of
amino acids from amino acids 1130-1146 or amino acids 1191-1198 of
SEQ ID NO: 5 can be replaced with glycine. In other aspects of this
embodiment, contiguous amino acid substitutions of hydrophobic
amino acids from amino acids 1130-1146 or amino acids 1191-1198 of
SEQ ID NO: 5 can be replaced with phenylalanine. In yet other
aspects of this embodiment, a modified BoNT/E H.sub.CC targeting
domain comprising a .beta.-trefoil fold domain with enhanced
binding activity comprises a polypeptide having, e.g., at most one,
two, three, four, five, six, seven, eight, nine or 10 contiguous
amino acid deletions relative to amino acids 1130-1146 or amino
acids 1191-1198 of SEQ ID NO: 5. In other aspects of this
embodiment, a modified BoNT/E H.sub.CC targeting domain comprising
a .beta.-trefoil fold domain with enhanced binding activity
comprises a polypeptide having, e.g., at least one, two, three,
four, five, six, seven, eight, nine or 10 contiguous amino acid
deletions relative to amino acids 1130-1146 or amino acids
1191-1198 of SEQ ID NO: 5. In still other aspects of this
embodiment, a modified BoNT/E H.sub.CC targeting domain comprising
a .beta.-trefoil fold domain with enhanced binding activity
comprises a polypeptide having, e.g., at most one, two, three,
four, five, six, seven, eight, nine or 10 contiguous amino acid
additions relative to amino acids 1130-1146 or amino acids
1191-1198 of SEQ ID NO: 5. In other aspects of this embodiment, a
modified BoNT/E H.sub.CC targeting domain comprising a
.beta.-trefoil fold domain with enhanced binding activity comprises
a polypeptide having, e.g., at least one, two, three, four, five,
six, seven, eight, nine or 10 contiguous amino acid additions
relative to amino acids 1130-1146 or amino acids 1191-1198 of SEQ
ID NO: 5.
[0301] In other aspects of this embodiment, a modified BoNT/E
H.sub.CC targeting domain with enhanced binding activity comprises
a substitution of amino acid Trp 1076, Gly 1077, Leu 1080, Tyr
1086, Tyr 1087, Gly 1124, Ile 1129, Asp 1146, Glu 1172, Phe 1213,
Ser 1221, Trp 1223, Tyr 1224, H is 1227, Gly 1236 or Trp 1239, or
any combination thereof, the substitution enhancing the binding
activity of the modified BoNT/E H.sub.CC targeting domain. In other
aspects of this embodiment, a modified BoNT/E H.sub.CC targeting
domain with enhanced binding activity comprises a deletion of amino
acid Trp 1076, Gly 1077, Leu 1080, Tyr 1086, Tyr 1087, Gly 1124,
Ile 1129, Asp 1146, Glu 1172, Phe 1213, Ser 1221, Trp 1223, Tyr
1224, His 1227, Gly 1236 or Trp 1239, or any combination thereof,
the deletion enhancing the binding activity of the modified BoNT/E
H.sub.CC targeting domain.
[0302] In another embodiment, a modified Clostridial toxin
comprising a targeting domain with enhanced binding activity
comprises a modified BoNT/F H.sub.CC targeting domain with enhanced
binding activity. In an aspect of this embodiment, a modified
Clostridial toxin comprises a modified BoNT/F H.sub.CC targeting
domain with an increased binding affinity or a modified BoNT/F
H.sub.CC targeting domain fragment with an increased binding
affinity. In another aspect of this embodiment, a modified
Clostridial toxin comprises a modified BoNT/F H.sub.CC targeting
domain with an increased binding specificity or a modified BoNT/F
H.sub.CC targeting domain fragment with an increased binding
specificity.
[0303] In another embodiment, a modified BoNT/F H.sub.CC targeting
domain with enhanced binding activity comprises a modification of
amino acids 1106-1274 of SEQ ID NO: 6. In another aspect of this
embodiment, a modified BoNT/F H.sub.CC targeting domain with an
altered cell binding capability comprises a modified .alpha.-fold
motif of a .beta.-trefoil domain of a BoNT/F H.sub.CC targeting
domain, a modified .beta.-fold motif of a .beta.-trefoil domain of
a BoNT/F H.sub.CC targeting domain, or a modified .gamma.-fold
motif of a .beta.-trefoil domain of a BoNT/F H.sub.CC targeting
domain. In another aspect of this embodiment, a modified BoNT/F
H.sub.CC targeting domain with an altered cell binding capability
comprises a modification to amino acids 1106-1152, amino acids
1172-1213, or amino acids 1222-1274 of SEQ ID NO: 6.
[0304] In other aspects of this embodiment, a modified BoNT/F
H.sub.CC targeting domain comprising a .beta.-trefoil fold domain
with enhanced binding activity comprises a polypeptide having,
e.g., at least 70% amino acid identity with amino acids 1106-1152,
amino acids 1172-1213, or amino acids 1222-1274 of SEQ ID NO: 6, at
least 75% amino acid identity with amino acids 1106-1152, amino
acids 1172-1213, or amino acids 1222-1274 of SEQ ID NO: 6, at least
80% amino acid identity with amino acids 1106-1152, amino acids
1172-1213, or amino acids 1222-1274 of SEQ ID NO: 6, at least 85%
amino acid identity with amino acids 1106-1152, amino acids
1172-1213, or amino acids 1222-1274 of SEQ ID NO: 6, at least 90%
amino acid identity with amino acids 1106-1152, amino acids
1172-1213, or amino acids 1222-1274 of SEQ ID NO: 6 or at least 95%
amino acid identity with amino acids 1106-1152, amino acids
1172-1213, or amino acids 1222-1274 of SEQ ID NO: 6. In yet other
aspects of this embodiment, a modified BoNT/F H.sub.CC targeting
domain comprising a .beta.-trefoil fold domain with enhanced
binding activity comprises a polypeptide having, e.g., at most 70%
amino acid identity with amino acids 1106-1152, amino acids
1172-1213, or amino acids 1222-1274 of SEQ ID NO: 6, at most 75%
amino acid identity with amino acids 1106-1152, amino acids
1172-1213, or amino acids 1222-1274 of SEQ ID NO: 6, at most 80%
amino acid identity with amino acids 1106-1152, amino acids
1172-1213, or amino acids 1222-1274 of SEQ ID NO: 6, at most 85%
amino acid identity with amino acids 1106-1152, amino acids
1172-1213, or amino acids 1222-1274 of SEQ ID NO: 6, at most 90%
amino acid identity with amino acids 1106-1152, amino acids
1172-1213, or amino acids 1222-1274 of SEQ ID NO: 6 or at most 95%
amino acid identity with amino acids 1106-1152, amino acids
1172-1213, or amino acids 1222-1274 of SEQ ID NO: 6.
[0305] In other aspects of this embodiment, a modified BoNT/F
H.sub.CC targeting domain comprising a .beta.-trefoil fold domain
with enhanced binding activity comprises a polypeptide having,
e.g., at most one, two, three, four, five, six, seven, eight, nine,
10 or 20 non-contiguous amino acid substitutions relative to amino
acids 1106-1152, amino acids 1172-1213, or amino acids 1222-1274 of
SEQ ID NO: 6. In other aspects of this embodiment, a modified
BoNT/F H.sub.CC targeting domain with enhanced binding activity
comprises a polypeptide having, e.g., at least one, two, three,
four, five, six, seven, eight, nine, 10 or 20 non-contiguous amino
acid substitutions relative to amino acids 1106-1152, amino acids
1172-1213, or amino acids 1222-1274 of SEQ ID NO: 6. In yet other
aspects of this embodiment, a modified BoNT/F H.sub.CC targeting
domain comprising a .beta.-trefoil fold domain with enhanced
binding activity comprises a polypeptide having, e.g., at most one,
two, three, four, five, six, seven, eight, nine, 10 or 20
non-contiguous amino acid deletions relative to amino acids
1106-1152, amino acids 1172-1213, or amino acids 1222-1274 of SEQ
ID NO: 6. In other aspects of this embodiment, a modified BoNT/F
H.sub.CC targeting domain comprising a .beta.-trefoil fold domain
with enhanced binding activity comprises a polypeptide having,
e.g., at least one, two, three, four, five, six, seven, eight,
nine, 10 or 20 non-contiguous amino acid deletions relative to
amino acids 1106-1152, amino acids 1172-1213, or amino acids
1222-1274 of SEQ ID NO: 6. In still other aspects of this
embodiment, a modified BoNT/F H.sub.CC targeting domain comprising
a .beta.-trefoil fold domain with enhanced binding activity
comprises a polypeptide having, e.g., at most one, two, three,
four, five, six, seven, eight, nine, 10 or 20 non-contiguous amino
acid additions relative to amino acids 1106-1152, amino acids
1172-1213, or amino acids 1222-1274 of SEQ ID NO: 6. In other
aspects of this embodiment, a modified BoNT/F H.sub.CC targeting
domain comprising a .beta.-trefoil fold domain with enhanced
binding activity comprises a polypeptide having, e.g., at least
one, two, three, four, five, six, seven, eight, nine, 10 or 20
non-contiguous amino acid additions relative to amino acids
1106-1152, amino acids 1172-1213, or amino acids 1222-1274 of SEQ
ID NO: 6.
[0306] In other aspects of this embodiment, a modified BoNT/F
H.sub.CC targeting domain comprising a .beta.-trefoil fold domain
with enhanced binding activity comprises a polypeptide having,
e.g., at most one, two, three, four, five, six, seven, eight, nine,
10 or 20 contiguous amino acid substitutions relative to amino
acids 1106-1152, amino acids 1172-1213, or amino acids 1222-1274 of
SEQ ID NO: 6. In other aspects of this embodiment, a modified
BoNT/F H.sub.CC targeting domain comprising a .beta.-trefoil fold
domain with enhanced binding activity comprises a polypeptide
having, e.g., at least one, two, three, four, five, six, seven,
eight, nine, 10 or 20 contiguous amino acid substitutions relative
to amino acids 1106-1152, amino acids 1172-1213, or amino acids
1222-1274 of SEQ ID NO: 6. In yet other aspects of this embodiment,
a modified BoNT/F H.sub.CC targeting domain comprising a
.beta.-trefoil fold domain with enhanced binding activity comprises
a polypeptide having, e.g., at most one, two, three, four, five,
six, seven, eight, nine, 10 or 20 contiguous amino acid deletions
relative to amino acids 1106-1152, amino acids 1172-1213, or amino
acids 1222-1274 of SEQ ID NO: 6. In other aspects of this
embodiment, a modified BoNT/F H.sub.CC targeting domain comprising
a .beta.-trefoil fold domain with enhanced binding activity
comprises a polypeptide having, e.g., at least one, two, three,
four, five, six, seven, eight, nine, 10 or 20 contiguous amino acid
deletions relative to amino acids 1106-1152, amino acids 1172-1213,
or amino acids 1222-1274 of SEQ ID NO: 6. In still other aspects of
this embodiment, a modified BoNT/F H.sub.CC targeting domain
comprising a .beta.-trefoil fold domain with enhanced binding
activity comprises a polypeptide having, e.g., at most one, two,
three, four, five, six, seven, eight, nine, 10 or 20 contiguous
amino acid additions relative to amino acids 1106-1152, amino acids
1172-1213, or amino acids 1222-1274 of SEQ ID NO: 6. In other
aspects of this embodiment, a modified BoNT/F H.sub.CC targeting
domain comprising a .beta.-trefoil fold domain with enhanced
binding activity comprises a polypeptide having, e.g., at least
one, two, three, four, five, six, seven, eight, nine, 10 or 20
contiguous amino acid additions relative to amino acids 1106-1152,
amino acids 1172-1213, or amino acids 1222-1274 of SEQ ID NO:
6.
[0307] In another embodiment, a modified BoNT/F H.sub.CC targeting
domain with enhanced binding activity comprises a modified BoNT/F
H.sub.CC targeting domain with enhanced binding activity of
comprises a modified .beta.4/.beta.5 hairpin turn of a
.beta.-trefoil domain of a BoNT/F H.sub.CC targeting domain or a
.beta.8/.beta.9 hairpin turn of a .beta.-trefoil domain of a BoNT/F
H.sub.CC targeting domain. In another aspect of this embodiment, a
modified BoNT/F H.sub.CC targeting domain with enhanced binding
activity comprises a modification of amino acids 1153-1171 or amino
acids 1214-1221 of SEQ ID NO: 6.
[0308] In other aspects of this embodiment, a modified BoNT/F
H.sub.CC targeting domain comprising a .beta.-trefoil fold domain
with enhanced binding activity comprises a polypeptide having,
e.g., at least 70% amino acid identity with amino acids 1153-1171
or amino acids 1214-1221 of SEQ ID NO: 6, at least 75% amino acid
identity with amino acids 1153-1171 or amino acids 1214-1221 of SEQ
ID NO: 6, at least 80% amino acid identity with amino acids
1153-1171 or amino acids 1214-1221 of SEQ ID NO: 6, at least 85%
amino acid identity with amino acids 1153-1171 or amino acids
1214-1221 of SEQ ID NO: 6, at least 90% amino acid identity with
amino acids 1153-1171 or amino acids 1214-1221 of SEQ ID NO: 6 or
at least 95% amino acid identity with amino acids 1153-1171 or
amino acids 1214-1221 of SEQ ID NO: 6. In yet other aspects of this
embodiment, a modified BoNT/F H.sub.CC targeting domain comprising
a .beta.-trefoil fold domain with enhanced binding activity
comprises a polypeptide having, e.g., at most 70% amino acid
identity with amino acids 1153-1171 or amino acids 1214-1221 of SEQ
ID NO: 6, at most 75% amino acid identity with amino acids
1153-1171 or amino acids 1214-1221 of SEQ ID NO: 6, at most 80%
amino acid identity with amino acids 1153-1171 or amino acids
1214-1221 of SEQ ID NO: 6, at most 85% amino acid identity with
amino acids 1153-1171 or amino acids 1214-1221 of SEQ ID NO: 6, at
most 90% amino acid identity with amino acids 1153-1171 or amino
acids 1214-1221 of SEQ ID NO: 6 or at most 95% amino acid identity
with amino acids 1153-1171 or amino acids 1214-1221 of SEQ ID NO:
6.
[0309] In other aspects of this embodiment, a modified BoNT/F
H.sub.CC targeting domain comprising a 11-trefoil fold domain with
enhanced binding activity comprises a polypeptide having, e.g., at
most one, two, three, four, five, six, seven, eight, nine or 10
non-contiguous amino acid substitutions relative to amino acids
1153-1171 or amino acids 1214-1221 of SEQ ID NO: 6. In other
aspects of this embodiment, a modified BoNT/F H.sub.CC targeting
domain comprises a polypeptide having, e.g., at least one, two,
three, four, five, six, seven, eight, nine or 10 non-contiguous
amino acid substitutions relative to amino acids 1153-1171 or amino
acids 1214-1221 of SEQ ID NO: 6. In other aspects of this
embodiment, a non-contiguous amino acid substitution of any amino
acid from amino acids 1153-1171 or amino acids 1214-1221 of SEQ ID
NO: 6 can be replaced with glycine. In other aspects of this
embodiment, a non-contiguous amino acid substitution of any
hydrophobic amino acid from amino acids 1153-1171 or amino acids
1214-1221 of SEQ ID NO: 6 can be replaced with phenylalanine. In
yet other aspects of this embodiment, a modified BoNT/F H.sub.CC
targeting domain comprising a .beta.-trefoil fold domain with
enhanced binding activity comprises a polypeptide having, e.g., at
most one, two, three, four, five, six, seven, eight, nine or 10
non-contiguous amino acid deletions relative to amino acids
1153-1171 or amino acids 1214-1221 of SEQ ID NO: 6. In other
aspects of this embodiment, a modified BoNT/F H.sub.CC targeting
domain comprising a .beta.-trefoil fold domain with enhanced
binding activity comprises a polypeptide having, e.g., at least
one, two, three, four, five, six, seven, eight, nine or 10
non-contiguous amino acid deletions relative to amino acids
1153-1171 or amino acids 1214-1221 of SEQ ID NO: 6. In still other
aspects of this embodiment, a modified BoNT/F H.sub.CC targeting
domain comprising a .beta.-trefoil fold domain with enhanced
binding activity comprises a polypeptide having, e.g., at most one,
two, three, four, five, six, seven, eight, nine or 10
non-contiguous amino acid additions relative to amino acids
1153-1171 or amino acids 1214-1221 of SEQ ID NO: 6. In other
aspects of this embodiment, a modified BoNT/F H.sub.CC targeting
domain comprising a .beta.-trefoil fold domain with enhanced
binding activity comprises a polypeptide having, e.g., at least
one, two, three, four, five, six, seven, eight, nine or 10
non-contiguous amino acid additions relative to amino acids
1153-1171 or amino acids 1214-1221 of SEQ ID NO: 6.
[0310] In other aspects of this embodiment, a modified BoNT/F
H.sub.CC targeting domain comprising a .beta.-trefoil fold domain
with enhanced binding activity comprises a polypeptide having,
e.g., at most one, two, three, four, five, six, seven, eight, nine
or 10 contiguous amino acid substitutions relative to amino acids
1153-1171 or amino acids 1214-1221 of SEQ ID NO: 6. In other
aspects of this embodiment, a modified BoNT/F H.sub.CC targeting
domain comprising a .beta.-trefoil fold domain with enhanced
binding activity comprises a polypeptide having, e.g., at least
one, two, three, four, five, six, seven, eight, nine or 10
contiguous amino acid substitutions relative to amino acids
1153-1171 or amino acids 1214-1221 of SEQ ID NO: 6. In other
aspects of this embodiment, contiguous amino acid substitutions of
amino acids from amino acids 1153-1171 or amino acids 1214-1221 of
SEQ ID NO: 6 can be replaced with glycine. In other aspects of this
embodiment, contiguous amino acid substitutions of hydrophobic
amino acids from amino acids 1153-1171 or amino acids 1214-1221 of
SEQ ID NO: 6 can be replaced with phenylalanine. In yet other
aspects of this embodiment, a modified BoNT/F H.sub.CC targeting
domain comprising a .beta.-trefoil fold domain with enhanced
binding activity comprises a polypeptide having, e.g., at most one,
two, three, four, five, six, seven, eight, nine or 10 contiguous
amino acid deletions relative to amino acids 1153-1171 or amino
acids 1214-1221 of SEQ ID NO: 6. In other aspects of this
embodiment, a modified BoNT/F H.sub.CC targeting domain comprising
a .beta.-trefoil fold domain with enhanced binding activity
comprises a polypeptide having, e.g., at least one, two, three,
four, five, six, seven, eight, nine or 10 contiguous amino acid
deletions relative to amino acids 1153-1171 or amino acids
1214-1221 of SEQ ID NO: 6. In still other aspects of this
embodiment, a modified BoNT/F H.sub.CC targeting domain comprising
a .beta.-trefoil fold domain with enhanced binding activity
comprises a polypeptide having, e.g., at most one, two, three,
four, five, six, seven, eight, nine or 10 contiguous amino acid
additions relative to amino acids 1153-1171 or amino acids
1214-1221 of SEQ ID NO: 6. In other aspects of this embodiment, a
modified BoNT/F H.sub.CC targeting domain comprising a
.beta.-trefoil fold domain with enhanced binding activity comprises
a polypeptide having, e.g., at least one, two, three, four, five,
six, seven, eight, nine or 10 contiguous amino acid additions
relative to amino acids 1153-1171 or amino acids 1214-1221 of SEQ
ID NO: 6.
[0311] In other aspects of this embodiment, a modified BoNT/F
H.sub.CC targeting domain with enhanced binding activity comprises
a substitution of amino acid Trp 1096, Gly 1097, Leu 1100, Tyr
1106, Tyr 1107, Gly 1147, Ile 1152, Asp 1171, Glu 1195, Phe 1237,
Ser 1245, Trp 1247, Tyr 1248, Asn 1251, Gly 1260 or Trp 1263, or
any combination thereof, the substitution enhancing the binding
activity of the modified BoNT/F H.sub.CC targeting domain. In other
aspects of this embodiment, a modified BoNT/F H.sub.CC targeting
domain with enhanced binding activity comprises a deletion of amino
acid Trp 1096, Gly 1097, Leu 1100, Tyr 1106, Tyr 1107, Gly 1147,
Ile 1152, Asp 1171, Glu 1195, Phe 1237, Ser 1245, Trp 1247, Tyr
1248, Asn 1251, Gly 1260 or Trp 1263, or any combination thereof,
the deletion enhancing the binding activity of the modified BoNT/F
H.sub.CC targeting domain.
[0312] In another embodiment, a modified Clostridial toxin
comprising a targeting domain with enhanced binding activity
comprises a modified BoNT/G H.sub.CC targeting domain with enhanced
binding activity. In an aspect of this embodiment, a modified
Clostridial toxin comprises a modified BoNT/G H.sub.CC targeting
domain with an increased binding affinity or a modified BoNT/G
H.sub.CC targeting domain fragment with an increased binding
affinity. In another aspect of this embodiment, a modified
Clostridial toxin comprises a modified BoNT/G H.sub.CC targeting
domain with an increased binding specificity or a modified BoNT/G
H.sub.CC targeting domain fragment with an increased binding
specificity.
[0313] In another embodiment, a modified BoNT/G H.sub.CC targeting
domain with enhanced binding activity comprises a modification of
amino acids 1106-1297 of SEQ ID NO: 7. In another aspect of this
embodiment, a modified BoNT/G H.sub.CC targeting domain with an
altered cell binding capability comprises a modified .alpha.-fold
motif of a .beta.-trefoil domain of a BoNT/G H.sub.CC targeting
domain, a modified .beta.-fold motif of a .beta.-trefoil domain of
a BoNT/G H.sub.CC targeting domain, or a modified .gamma.-fold
motif of a .beta.-trefoil domain of a BoNT/G H.sub.CC targeting
domain. In another aspect of this embodiment, a modified BoNT/G
H.sub.CC targeting domain with an altered cell binding capability
comprises a modification to amino acids 1106-1153, amino acids
1173-1218, or amino acids 1231-1297 of SEQ ID NO: 7.
[0314] In other aspects of this embodiment, a modified BoNT/G
H.sub.CC targeting domain comprising a 11-trefoil fold domain with
enhanced binding activity comprises a polypeptide having, e.g., at
least 70% amino acid identity with amino acids 1106-1153, amino
acids 1173-1218, or amino acids 1231-1297 of SEQ ID NO: 7, at least
75% amino acid identity with amino acids 1106-1153, amino acids
1173-1218, or amino acids 1231-1297 of SEQ ID NO: 7, at least 80%
amino acid identity with amino acids 1106-1153, amino acids
1173-1218, or amino acids 1231-1297 of SEQ ID NO: 7, at least 85%
amino acid identity with amino acids 1106-1153, amino acids
1173-1218, or amino acids 1231-1297 of SEQ ID NO: 7, at least 90%
amino acid identity with amino acids 1106-1153, amino acids
1173-1218, or amino acids 1231-1297 of SEQ ID NO: 7 or at least 95%
amino acid identity with amino acids 1106-1153, amino acids
1173-1218, or amino acids 1231-1297 of SEQ ID NO: 7. In yet other
aspects of this embodiment, a modified BoNT/G H.sub.CC targeting
domain comprising a .beta.-trefoil fold domain with enhanced
binding activity comprises a polypeptide having, e.g., at most 70%
amino acid identity with amino acids 1106-1153, amino acids
1173-1218, or amino acids 1231-1297 of SEQ ID NO: 7, at most 75%
amino acid identity with amino acids 1106-1153, amino acids
1173-1218, or amino acids 1231-1297 of SEQ ID NO: 7, at most 80%
amino acid identity with amino acids 1106-1153, amino acids
1173-1218, or amino acids 1231-1297 of SEQ ID NO: 7, at most 85%
amino acid identity with amino acids 1106-1153, amino acids
1173-1218, or amino acids 1231-1297 of SEQ ID NO: 7, at most 90%
amino acid identity with amino acids 1106-1153, amino acids
1173-1218, or amino acids 1231-1297 of SEQ ID NO: 7 or at most 95%
amino acid identity with amino acids 1106-1153, amino acids
1173-1218, or amino acids 1231-1297 of SEQ ID NO: 7.
[0315] In other aspects of this embodiment, a modified BoNT/G
H.sub.CC targeting domain comprising a .beta.-trefoil fold domain
with enhanced binding activity comprises a polypeptide having,
e.g., at most one, two, three, four, five, six, seven, eight, nine,
10 or 20 non-contiguous amino acid substitutions relative to amino
acids 1106-1153, amino acids 1173-1218, or amino acids 1231-1297 of
SEQ ID NO: 7. In other aspects of this embodiment, a modified
BoNT/G H.sub.CC targeting domain with enhanced binding activity
comprises a polypeptide having, e.g., at least one, two, three,
four, five, six, seven, eight, nine, 10 or 20 non-contiguous amino
acid substitutions relative to amino acids 1106-1153, amino acids
1173-1218, or amino acids 1231-1297 of SEQ ID NO: 7. In yet other
aspects of this embodiment, a modified BoNT/G H.sub.CC targeting
domain comprising a .beta.-trefoil fold domain with enhanced
binding activity comprises a polypeptide having, e.g., at most one,
two, three, four, five, six, seven, eight, nine, 10 or 20
non-contiguous amino acid deletions relative to amino acids
1106-1153, amino acids 1173-1218, or amino acids 1231-1297 of SEQ
ID NO: 7. In other aspects of this embodiment, a modified BoNT/G
H.sub.CC targeting domain comprising a .beta.-trefoil fold domain
with enhanced binding activity comprises a polypeptide having,
e.g., at least one, two, three, four, five, six, seven, eight,
nine, 10 or 20 non-contiguous amino acid deletions relative to
amino acids 1106-1153, amino acids 1173-1218, or amino acids
1231-1297 of SEQ ID NO: 7. In still other aspects of this
embodiment, a modified BoNT/G H.sub.CC targeting domain comprising
a .beta.-trefoil fold domain with enhanced binding activity
comprises a polypeptide having, e.g., at most one, two, three,
four, five, six, seven, eight, nine, 10 or 20 non-contiguous amino
acid additions relative to amino acids 1106-1153, amino acids
1173-1218, or amino acids 1231-1297 of SEQ ID NO: 7. In other
aspects of this embodiment, a modified BoNT/G H.sub.CC targeting
domain comprising a .beta.-trefoil fold domain with enhanced
binding activity comprises a polypeptide having, e.g., at least
one, two, three, four, five, six, seven, eight, nine, 10 or 20
non-contiguous amino acid additions relative to amino acids
1106-1153, amino acids 1173-1218, or amino acids 1231-1297 of SEQ
ID NO: 7.
[0316] In other aspects of this embodiment, a modified BoNT/G
H.sub.CC targeting domain comprising a .beta.-trefoil fold domain
with enhanced binding activity comprises a polypeptide having,
e.g., at most one, two, three, four, five, six, seven, eight, nine,
10 or 20 contiguous amino acid substitutions relative to amino
acids 1106-1153, amino acids 1173-1218, or amino acids 1231-1297 of
SEQ ID NO: 7. In other aspects of this embodiment, a modified
BoNT/G H.sub.CC targeting domain comprising a .beta.-trefoil fold
domain with enhanced binding activity comprises a polypeptide
having, e.g., at least one, two, three, four, five, six, seven,
eight, nine, 10 or 20 contiguous amino acid substitutions relative
to amino acids 1106-1153, amino acids 1173-1218, or amino acids
1231-1297 of SEQ ID NO: 7. In yet other aspects of this embodiment,
a modified BoNT/G H.sub.CC targeting domain comprising a
.beta.-trefoil fold domain with enhanced binding activity comprises
a polypeptide having, e.g., at most one, two, three, four, five,
six, seven, eight, nine, 10 or 20 contiguous amino acid deletions
relative to amino acids 1106-1153, amino acids 1173-1218, or amino
acids 1231-1297 of SEQ ID NO: 7. In other aspects of this
embodiment, a modified BoNT/G H.sub.CC targeting domain comprising
a .beta.-trefoil fold domain with enhanced binding activity
comprises a polypeptide having, e.g., at least one, two, three,
four, five, six, seven, eight, nine, 10 or 20 contiguous amino acid
deletions relative to amino acids 1106-1153, amino acids 1173-1218,
or amino acids 1231-1297 of SEQ ID NO: 7. In still other aspects of
this embodiment, a modified BoNT/G H.sub.CC targeting domain
comprising a .beta.-trefoil fold domain with enhanced binding
activity comprises a polypeptide having, e.g., at most one, two,
three, four, five, six, seven, eight, nine, 10 or 20 contiguous
amino acid additions relative to amino acids 1106-1153, amino acids
1173-1218, or amino acids 1231-1297 of SEQ ID NO: 7. In other
aspects of this embodiment, a modified BoNT/G H.sub.CC targeting
domain comprising a .beta.-trefoil fold domain with enhanced
binding activity comprises a polypeptide having, e.g., at least
one, two, three, four, five, six, seven, eight, nine, 10 or 20
contiguous amino acid additions relative to amino acids 1106-1153,
amino acids 1173-1218, or amino acids 1231-1297 of SEQ ID NO:
7.
[0317] In another embodiment, a modified BoNT/G H.sub.CC targeting
domain with enhanced binding activity comprises a modified BoNT/G
H.sub.CC targeting domain with enhanced binding activity of
comprises a modified .beta.4/.beta.5 hairpin turn of a
.beta.-trefoil domain of a BoNT/G H.sub.CC targeting domain or a
.beta.8/.beta.9 hairpin turn of a .beta.-trefoil domain of a BoNT/G
H.sub.CC targeting domain. In another aspect of this embodiment, a
modified BoNT/G H.sub.CC targeting domain with enhanced binding
activity comprises a modification of amino acids 1154-1172 or amino
acids 1219-1230 of SEQ ID NO: 7.
[0318] In other aspects of this embodiment, a modified BoNT/G
H.sub.CC targeting domain comprising a 11-trefoil fold domain with
enhanced binding activity comprises a polypeptide having, e.g., at
least 70% amino acid identity with amino acids 1154-1172 or amino
acids 1219-1230 of SEQ ID NO: 7, at least 75% amino acid identity
with amino acids 1154-1172 or amino acids 1219-1230 of SEQ ID NO:
7, at least 80% amino acid identity with amino acids 1154-1172 or
amino acids 1219-1230 of SEQ ID NO: 7, at least 85% amino acid
identity with amino acids 1154-1172 or amino acids 1219-1230 of SEQ
ID NO: 7, at least 90% amino acid identity with amino acids
1154-1172 or amino acids 1219-1230 of SEQ ID NO: 7 or at least 95%
amino acid identity with amino acids 1154-1172 or amino acids
1219-1230 of SEQ ID NO: 7. In yet other aspects of this embodiment,
a modified BoNT/G H.sub.CC targeting domain comprising a
.beta.-trefoil fold domain with enhanced binding activity comprises
a polypeptide having, e.g., at most 70% amino acid identity with
amino acids 1154-1172 or amino acids 1219-1230 of SEQ ID NO: 7, at
most 75% amino acid identity with amino acids 1154-1172 or amino
acids 1219-1230 of SEQ ID NO: 7, at most 80% amino acid identity
with amino acids 1154-1172 or amino acids 1219-1230 of SEQ ID NO:
7, at most 85% amino acid identity with amino acids 1154-1172 or
amino acids 1219-1230 of SEQ ID NO: 7, at most 90% amino acid
identity with amino acids 1154-1172 or amino acids 1219-1230 of SEQ
ID NO: 7 or at most 95% amino acid identity with amino acids
1154-1172 or amino acids 1219-1230 of SEQ ID NO: 7.
[0319] In other aspects of this embodiment, a modified BoNT/G
H.sub.CC targeting domain comprising a .beta.-trefoil fold domain
with enhanced binding activity comprises a polypeptide having,
e.g., at most one, two, three, four, five, six, seven, eight, nine
or 10 non-contiguous amino acid substitutions relative to amino
acids 1154-1172 or amino acids 1219-1230 of SEQ ID NO: 7. In other
aspects of this embodiment, a modified BoNT/G H.sub.CC targeting
domain comprises a polypeptide having, e.g., at least one, two,
three, four, five, six, seven, eight, nine or 10 non-contiguous
amino acid substitutions relative to amino acids 1154-1172 or amino
acids 1219-1230 of SEQ ID NO: 7. In other aspects of this
embodiment, a non-contiguous amino acid substitution of any amino
acid from amino acids 1154-1172 or amino acids 1219-1230 of SEQ ID
NO: 7 can be replaced with glycine. In other aspects of this
embodiment, a non-contiguous amino acid substitution of any
hydrophobic amino acid from amino acids 1154-1172 or amino acids
1219-1230 of SEQ ID NO: 7 can be replaced with phenylalanine. In
yet other aspects of this embodiment, a modified BoNT/G H.sub.CC
targeting domain comprising a .beta.-trefoil fold domain with
enhanced binding activity comprises a polypeptide having, e.g., at
most one, two, three, four, five, six, seven, eight, nine or 10
non-contiguous amino acid deletions relative to amino acids
1154-1172 or amino acids 1219-1230 of SEQ ID NO: 7. In other
aspects of this embodiment, a modified BoNT/G H.sub.CC targeting
domain comprising a .beta.-trefoil fold domain with enhanced
binding activity comprises a polypeptide having, e.g., at least
one, two, three, four, five, six, seven, eight, nine or 10
non-contiguous amino acid deletions relative to amino acids
1154-1172 or amino acids 1219-1230 of SEQ ID NO: 7. In still other
aspects of this embodiment, a modified BoNT/G H.sub.CC targeting
domain comprising a .beta.-trefoil fold domain with enhanced
binding activity comprises a polypeptide having, e.g., at most one,
two, three, four, five, six, seven, eight, nine or 10
non-contiguous amino acid additions relative to amino acids
1154-1172 or amino acids 1219-1230 of SEQ ID NO: 7. In other
aspects of this embodiment, a modified BoNT/G H.sub.CC targeting
domain comprising a .beta.-trefoil fold domain with enhanced
binding activity comprises a polypeptide having, e.g., at least
one, two, three, four, five, six, seven, eight, nine or 10
non-contiguous amino acid additions relative to amino acids
1154-1172 or amino acids 1219-1230 of SEQ ID NO: 7.
[0320] In other aspects of this embodiment, a modified BoNT/G
H.sub.CC targeting domain comprising a .beta.-trefoil fold domain
with enhanced binding activity comprises a polypeptide having,
e.g., at most one, two, three, four, five, six, seven, eight, nine
or 10 contiguous amino acid substitutions relative to amino acids
1154-1172 or amino acids 1219-1230 of SEQ ID NO: 7. In other
aspects of this embodiment, a modified BoNT/G H.sub.CC targeting
domain comprising a .beta.-trefoil fold domain with enhanced
binding activity comprises a polypeptide having, e.g., at least
one, two, three, four, five, six, seven, eight, nine or 10
contiguous amino acid substitutions relative to amino acids
1154-1172 or amino acids 1219-1230 of SEQ ID NO: 7. In other
aspects of this embodiment, contiguous amino acid substitutions of
amino acids from amino acids 1154-1172 or amino acids 1219-1230 of
SEQ ID NO: 7 can be replaced with glycine. In other aspects of this
embodiment, contiguous amino acid substitutions of hydrophobic
amino acids from amino acids 1154-1172 or amino acids 1219-1230 of
SEQ ID NO: 7 can be replaced with phenylalanine. In yet other
aspects of this embodiment, a modified BoNT/G H.sub.CC targeting
domain comprising a .beta.-trefoil fold domain with enhanced
binding activity comprises a polypeptide having, e.g., at most one,
two, three, four, five, six, seven, eight, nine or 10 contiguous
amino acid deletions relative to amino acids 1154-1172 or amino
acids 1219-1230 of SEQ ID NO: 7. In other aspects of this
embodiment, a modified BoNT/G H.sub.CC targeting domain comprising
a .beta.-trefoil fold domain with enhanced binding activity
comprises a polypeptide having, e.g., at least one, two, three,
four, five, six, seven, eight, nine or 10 contiguous amino acid
deletions relative to amino acids 1154-1172 or amino acids
1219-1230 of SEQ ID NO: 7. In still other aspects of this
embodiment, a modified BoNT/G H.sub.CC targeting domain comprising
a .beta.-trefoil fold domain with enhanced binding activity
comprises a polypeptide having, e.g., at most one, two, three,
four, five, six, seven, eight, nine or 10 contiguous amino acid
additions relative to amino acids 1154-1172 or amino acids
1219-1230 of SEQ ID NO: 7. In other aspects of this embodiment, a
modified BoNT/G H.sub.CC targeting domain comprising a
.beta.-trefoil fold domain with enhanced binding activity comprises
a polypeptide having, e.g., at least one, two, three, four, five,
six, seven, eight, nine or 10 contiguous amino acid additions
relative to amino acids 1154-1172 or amino acids 1219-1230 of SEQ
ID NO: 7.
[0321] In other aspects of this embodiment, a modified BoNT/G
H.sub.CC targeting domain with enhanced binding activity comprises
a substitution of amino acid Trp 1096, Gly 1097, Leu 1100, Tyr
1106, Tyr 1107, Gly 1148, Ile 1153, Asp 1172, Gln 1198, Ile 1245,
Ser 1266, Trp 1268, Tyr 1269, Arg 1272, Gly 1283 or Trp 1285, or
any combination thereof, the substitution enhancing the binding
activity of the modified BoNT/G H.sub.CC targeting domain. In other
aspects of this embodiment, a modified BoNT/G H.sub.CC targeting
domain with enhanced binding activity comprises a deletion of amino
acid Trp 1096, Gly 1097, Leu 1100, Tyr 1106, Tyr 1107, Gly 1148,
Ile 1153, Asp 1172, Gln 1198, Ile 1245, Ser 1266, Trp 1268, Tyr
1269, Arg 1272, Gly 1283 or Trp 1285, or any combination thereof,
the deletion enhancing the binding activity of the modified BoNT/G
H.sub.CC targeting domain.
[0322] In another embodiment, a modified Clostridial toxin
comprising a targeting domain with enhanced binding activity
comprises a modified TeNT H.sub.CC targeting domain with enhanced
binding activity. In an aspect of this embodiment, a modified
Clostridial toxin comprises a modified TeNT H.sub.CC targeting
domain with an increased binding affinity or a modified TeNT
H.sub.CC targeting domain fragment with an increased binding
affinity. In another aspect of this embodiment, a modified
Clostridial toxin comprises a modified TeNT H.sub.CC targeting
domain with an increased binding specificity or a modified TeNT
H.sub.CC targeting domain fragment with an increased binding
specificity.
[0323] In another embodiment, a modified TeNT H.sub.CC targeting
domain with enhanced binding activity comprises a modification of
amino acids 1128-1315 of SEQ ID NO: 8. In another aspect of this
embodiment, a modified TeNT H.sub.CC targeting domain with an
altered cell binding capability comprises a modified .alpha.-fold
motif of a .beta.-trefoil domain of a TeNT H.sub.CC targeting
domain, a modified .beta.-fold motif of a .beta.-trefoil domain of
a TeNT H.sub.CC targeting domain, or a modified .gamma.-fold motif
of a .beta.-trefoil domain of a TeNT H.sub.CC targeting domain. In
another aspect of this embodiment, a modified TeNT H.sub.CC
targeting domain with an altered cell binding capability comprises
a modification to amino acids 1128-1177, amino acids 1195-1240, or
amino acids 1255-1315 of SEQ ID NO: 8.
[0324] In other aspects of this embodiment, a modified TeNT
H.sub.CC targeting domain comprising a .beta.-trefoil fold domain
with enhanced binding activity comprises a polypeptide having,
e.g., at least 70% amino acid identity with amino acids 1128-1177,
amino acids 1195-1240, or amino acids 1255-1315 of SEQ ID NO: 8, at
least 75% amino acid identity with amino acids 1128-1177, amino
acids 1195-1240, or amino acids 1255-1315 of SEQ ID NO: 8, at least
80% amino acid identity with amino acids 1128-1177, amino acids
1195-1240, or amino acids 1255-1315 of SEQ ID NO: 8, at least 85%
amino acid identity with amino acids 1128-1177, amino acids
1195-1240, or amino acids 1255-1315 of SEQ ID NO: 8, at least 90%
amino acid identity with amino acids 1128-1177, amino acids
1195-1240, or amino acids 1255-1315 of SEQ ID NO: 8 or at least 95%
amino acid identity with amino acids 1128-1177, amino acids
1195-1240, or amino acids 1255-1315 of SEQ ID NO: 8. In yet other
aspects of this embodiment, a modified TeNT H.sub.CC targeting
domain comprising a .beta.-trefoil fold domain with enhanced
binding activity comprises a polypeptide having, e.g., at most 70%
amino acid identity with amino acids 1128-1177, amino acids
1195-1240, or amino acids 1255-1315 of SEQ ID NO: 8, at most 75%
amino acid identity with amino acids 1128-1177, amino acids
1195-1240, or amino acids 1255-1315 of SEQ ID NO: 8, at most 80%
amino acid identity with amino acids 1128-1177, amino acids
1195-1240, or amino acids 1255-1315 of SEQ ID NO: 8, at most 85%
amino acid identity with amino acids 1128-1177, amino acids
1195-1240, or amino acids 1255-1315 of SEQ ID NO: 8, at most 90%
amino acid identity with amino acids 1128-1177, amino acids
1195-1240, or amino acids 1255-1315 of SEQ ID NO: 8 or at most 95%
amino acid identity with amino acids 1128-1177, amino acids
1195-1240, or amino acids 1255-1315 of SEQ ID NO: 8.
[0325] In other aspects of this embodiment, a modified TeNT
H.sub.CC targeting domain comprising a .beta.-trefoil fold domain
with enhanced binding activity comprises a polypeptide having,
e.g., at most one, two, three, four, five, six, seven, eight, nine,
10 or 20 non-contiguous amino acid substitutions relative to amino
acids 1128-1177, amino acids 1195-1240, or amino acids 1255-1315 of
SEQ ID NO: 8. In other aspects of this embodiment, a modified TeNT
H.sub.CC targeting domain with enhanced binding activity comprises
a polypeptide having, e.g., at least one, two, three, four, five,
six, seven, eight, nine, 10 or 20 non-contiguous amino acid
substitutions relative to amino acids 1128-1177, amino acids
1195-1240, or amino acids 1255-1315 of SEQ ID NO: 8. In yet other
aspects of this embodiment, a modified TeNT H.sub.CC targeting
domain comprising a .beta.-trefoil fold domain with enhanced
binding activity comprises a polypeptide having, e.g., at most one,
two, three, four, five, six, seven, eight, nine, 10 or 20
non-contiguous amino acid deletions relative to amino acids
1128-1177, amino acids 1195-1240, or amino acids 1255-1315 of SEQ
ID NO: 8. In other aspects of this embodiment, a modified TeNT
H.sub.CC targeting domain comprising a .beta.-trefoil fold domain
with enhanced binding activity comprises a polypeptide having,
e.g., at least one, two, three, four, five, six, seven, eight,
nine, 10 or 20 non-contiguous amino acid deletions relative to
amino acids 1128-1177, amino acids 1195-1240, or amino acids
1255-1315 of SEQ ID NO: 8. In still other aspects of this
embodiment, a modified TeNT H.sub.CC targeting domain comprising a
.beta.-trefoil fold domain with enhanced binding activity comprises
a polypeptide having, e.g., at most one, two, three, four, five,
six, seven, eight, nine, 10 or 20 non-contiguous amino acid
additions relative to amino acids 1128-1177, amino acids 1195-1240,
or amino acids 1255-1315 of SEQ ID NO: 8. In other aspects of this
embodiment, a modified TeNT H.sub.CC targeting domain comprising a
.beta.-trefoil fold domain with enhanced binding activity comprises
a polypeptide having, e.g., at least one, two, three, four, five,
six, seven, eight, nine, 10 or 20 non-contiguous amino acid
additions relative to amino acids 1128-1177, amino acids 1195-1240,
or amino acids 1255-1315 of SEQ ID NO: 8.
[0326] In other aspects of this embodiment, a modified TeNT
H.sub.CC targeting domain comprising a .beta.-trefoil fold domain
with enhanced binding activity comprises a polypeptide having,
e.g., at most one, two, three, four, five, six, seven, eight, nine,
10 or 20 contiguous amino acid substitutions relative to amino
acids 1128-1177, amino acids 1195-1240, or amino acids 1255-1315 of
SEQ ID NO: 8. In other aspects of this embodiment, a modified TeNT
H.sub.CC targeting domain comprising a .beta.-trefoil fold domain
with enhanced binding activity comprises a polypeptide having,
e.g., at least one, two, three, four, five, six, seven, eight,
nine, 10 or 20 contiguous amino acid substitutions relative to
amino acids 1128-1177, amino acids 1195-1240, or amino acids
1255-1315 of SEQ ID NO: 8. In yet other aspects of this embodiment,
a modified TeNT H.sub.CC targeting domain comprising a
.beta.-trefoil fold domain with enhanced binding activity comprises
a polypeptide having, e.g., at most one, two, three, four, five,
six, seven, eight, nine, 10 or 20 contiguous amino acid deletions
relative to amino acids 1128-1177, amino acids 1195-1240, or amino
acids 1255-1315 of SEQ ID NO: 8. In other aspects of this
embodiment, a modified TeNT H.sub.CC targeting domain comprising a
.beta.-trefoil fold domain with enhanced binding activity comprises
a polypeptide having, e.g., at least one, two, three, four, five,
six, seven, eight, nine, 10 or 20 contiguous amino acid deletions
relative to amino acids 1128-1177, amino acids 1195-1240, or amino
acids 1255-1315 of SEQ ID NO: 8. In still other aspects of this
embodiment, a modified TeNT H.sub.CC targeting domain comprising a
.beta.-trefoil fold domain with enhanced binding activity comprises
a polypeptide having, e.g., at most one, two, three, four, five,
six, seven, eight, nine, 10 or 20 contiguous amino acid additions
relative to amino acids 1128-1177, amino acids 1195-1240, or amino
acids 1255-1315 of SEQ ID NO: 8. In other aspects of this
embodiment, a modified TeNT H.sub.CC targeting domain comprising a
.beta.-trefoil fold domain with enhanced binding activity comprises
a polypeptide having, e.g., at least one, two, three, four, five,
six, seven, eight, nine, 10 or 20 contiguous amino acid additions
relative to amino acids 1128-1177, amino acids 1195-1240, or amino
acids 1255-1315 of SEQ ID NO: 8.
[0327] In another embodiment, a modified TeNT H.sub.CC targeting
domain with enhanced binding activity comprises a modified TeNT
H.sub.CC targeting domain with enhanced binding activity of
comprises a modified .beta.4/.beta.5 hairpin turn of a
.beta.-trefoil domain of a TeNT H.sub.CC targeting domain or a
.beta.8/.beta.9 hairpin turn of a .beta.-trefoil domain of a TeNT
H.sub.CC targeting domain. In another aspect of this embodiment, a
modified TeNT H.sub.CC targeting domain with enhanced binding
activity comprises a modification of amino acids 1178-1194 or amino
acids 1241-1254 of SEQ ID NO: 8.
[0328] In other aspects of this embodiment, a modified TeNT
H.sub.CC targeting domain comprising a .beta.-trefoil fold domain
with enhanced binding activity comprises a polypeptide having,
e.g., at least 70% amino acid identity with amino acids 1178-1194
or amino acids 1241-1254 of SEQ ID NO: 8, at least 75% amino acid
identity with amino acids 1178-1194 or amino acids 1241-1254 of SEQ
ID NO: 8, at least 80% amino acid identity with amino acids
1178-1194 or amino acids 1241-1254 of SEQ ID NO: 8, at least 85%
amino acid identity with amino acids 1178-1194 or amino acids
1241-1254 of SEQ ID NO: 8, at least 90% amino acid identity with
amino acids 1178-1194 or amino acids 1241-1254 of SEQ ID NO: 8 or
at least 95% amino acid identity with amino acids 1178-1194 or
amino acids 1241-1254 of SEQ ID NO: 8. In yet other aspects of this
embodiment, a modified TeNT H.sub.CC targeting domain comprising a
.beta.-trefoil fold domain with enhanced binding activity comprises
a polypeptide having, e.g., at most 70% amino acid identity with
amino acids 1178-1194 or amino acids 1241-1254 of SEQ ID NO: 8, at
most 75% amino acid identity with amino acids 1178-1194 or amino
acids 1241-1254 of SEQ ID NO: 8, at most 80% amino acid identity
with amino acids 1178-1194 or amino acids 1241-1254 of SEQ ID NO:
8, at most 85% amino acid identity with amino acids 1178-1194 or
amino acids 1241-1254 of SEQ ID NO: 8, at most 90% amino acid
identity with amino acids 1178-1194 or amino acids 1241-1254 of SEQ
ID NO: 8 or at most 95% amino acid identity with amino acids
1178-1194 or amino acids 1241-1254 of SEQ ID NO: 8.
[0329] In other aspects of this embodiment, a modified TeNT
H.sub.CC targeting domain comprising a .beta.-trefoil fold domain
with enhanced binding activity comprises a polypeptide having,
e.g., at most one, two, three, four, five, six, seven, eight, nine
or 10 non-contiguous amino acid substitutions relative to amino
acids 1178-1194 or amino acids 1241-1254 of SEQ ID NO: 8. In other
aspects of this embodiment, a modified TeNT H.sub.CC targeting
domain comprises a polypeptide having, e.g., at least one, two,
three, four, five, six, seven, eight, nine or 10 non-contiguous
amino acid substitutions relative to amino acids 1178-1194 or amino
acids 1241-1254 of SEQ ID NO: 8. In other aspects of this
embodiment, a non-contiguous amino acid substitution of any amino
acid from amino acids 1178-1194 or amino acids 1241-1254 of SEQ ID
NO: 8 can be replaced with glycine. In other aspects of this
embodiment, a non-contiguous amino acid substitution of any
hydrophobic amino acid from amino acids 1178-1194 or amino acids
1241-1254 of SEQ ID NO: 8 can be replaced with phenylalanine. In
yet other aspects of this embodiment, a modified TeNT H.sub.CC
targeting domain comprising a .beta.-trefoil fold domain with
enhanced binding activity comprises a polypeptide having, e.g., at
most one, two, three, four, five, six, seven, eight, nine or 10
non-contiguous amino acid deletions relative to amino acids
1178-1194 or amino acids 1241-1254 of SEQ ID NO: 8. In other
aspects of this embodiment, a modified TeNT H.sub.CC targeting
domain comprising a .beta.-trefoil fold domain with enhanced
binding activity comprises a polypeptide having, e.g., at least
one, two, three, four, five, six, seven, eight, nine or 10
non-contiguous amino acid deletions relative to amino acids
1178-1194 or amino acids 1241-1254 of SEQ ID NO: 8. In still other
aspects of this embodiment, a modified TeNT H.sub.CC targeting
domain comprising a .beta.-trefoil fold domain with enhanced
binding activity comprises a polypeptide having, e.g., at most one,
two, three, four, five, six, seven, eight, nine or 10
non-contiguous amino acid additions relative to amino acids
1178-1194 or amino acids 1241-1254 of SEQ ID NO: 8. In other
aspects of this embodiment, a modified TeNT H.sub.CC targeting
domain comprising a .beta.-trefoil fold domain with enhanced
binding activity comprises a polypeptide having, e.g., at least
one, two, three, four, five, six, seven, eight, nine or 10
non-contiguous amino acid additions relative to amino acids
1178-1194 or amino acids 1241-1254 of SEQ ID NO: 8.
[0330] In other aspects of this embodiment, a modified TeNT
H.sub.CC targeting domain comprising a .beta.-trefoil fold domain
with enhanced binding activity comprises a polypeptide having,
e.g., at most one, two, three, four, five, six, seven, eight, nine
or 10 contiguous amino acid substitutions relative to amino acids
1178-1194 or amino acids 1241-1254 of SEQ ID NO: 8. In other
aspects of this embodiment, a modified TeNT H.sub.CC targeting
domain comprising a .beta.-trefoil fold domain with enhanced
binding activity comprises a polypeptide having, e.g., at least
one, two, three, four, five, six, seven, eight, nine or 10
contiguous amino acid substitutions relative to amino acids
1178-1194 or amino acids 1241-1254 of SEQ ID NO: 8. In other
aspects of this embodiment, contiguous amino acid substitutions of
amino acids from amino acids 1178-1194 or amino acids 1241-1254 of
SEQ ID NO: 8 can be replaced with glycine. In other aspects of this
embodiment, contiguous amino acid substitutions of hydrophobic
amino acids from amino acids 1178-1194 or amino acids 1241-1254 of
SEQ ID NO: 8 can be replaced with phenylalanine. In yet other
aspects of this embodiment, a modified TeNT H.sub.CC targeting
domain comprising a .beta.-trefoil fold domain with enhanced
binding activity comprises a polypeptide having, e.g., at most one,
two, three, four, five, six, seven, eight, nine or 10 contiguous
amino acid deletions relative to amino acids 1178-1194 or amino
acids 1241-1254 of SEQ ID NO: 8. In other aspects of this
embodiment, a modified TeNT H.sub.CC targeting domain comprising a
.beta.-trefoil fold domain with enhanced binding activity comprises
a polypeptide having, e.g., at least one, two, three, four, five,
six, seven, eight, nine or 10 contiguous amino acid deletions
relative to amino acids 1178-1194 or amino acids 1241-1254 of SEQ
ID NO: 8. In still other aspects of this embodiment, a modified
TeNT H.sub.CC targeting domain comprising a .beta.-trefoil fold
domain with enhanced binding activity comprises a polypeptide
having, e.g., at most one, two, three, four, five, six, seven,
eight, nine or 10 contiguous amino acid additions relative to amino
acids 1178-1194 or amino acids 1241-1254 of SEQ ID NO: 8. In other
aspects of this embodiment, a modified TeNT H.sub.CC targeting
domain comprising a .beta.-trefoil fold domain with enhanced
binding activity comprises a polypeptide having, e.g., at least
one, two, three, four, five, six, seven, eight, nine or 10
contiguous amino acid additions relative to amino acids 1178-1194
or amino acids 1241-1254 of SEQ ID NO: 8.
[0331] In other aspects of this embodiment, a modified TeNT
H.sub.CC targeting domain with enhanced binding activity comprises
a substitution of amino acid Trp 1118, Gly 1119, Leu 1122, Tyr
1128, Tyr 1129, Gly 1172, Ile 1177, Asp 1194, Asp 1222, Thr 1270,
Ser 1287, Trp 1289, Tyr 1290, H is 1293, Gly 1300 or Trp 1303, or
any combination thereof, the substitution enhancing the binding
activity of the modified TeNT H.sub.CC targeting domain. In other
aspects of this embodiment, a modified TeNT H.sub.CC targeting
domain with enhanced binding activity comprises a deletion of amino
acid Trp 1118, Gly 1119, Leu 1122, Tyr 1128, Tyr 1129, Gly 1172,
Ile 1177, Asp 1194, Asp 1222, Thr 1270, Ser 1287, Trp 1289, Tyr
1290, H is 1293, Gly 1300 or Trp 1303, or any combination thereof,
the deletion enhancing the binding activity of the modified TeNT
H.sub.CC targeting domain.
[0332] Another example of an enhanced targeting domain that
increases binding activity for an endogenous Clostridial toxin
receptor present on a naturally-occurring Clostridial toxin target
cell, includes, without limitation, non-toxin associated proteins
of Clostridial toxins, such as, e.g., non-toxic non-hemagglutinin
(NTNH), hemagglutinin-17 (HA-17), hemagglutinin-33 (HA-33) and
hemagglutinin-70 (HA-70). In vivo, Clostridial bacteria produce a
toxin complex comprising the approximately 150-kDa Clostridial
toxin and other proteins collectively called nontoxin associated
proteins (NAPs). Identified NAPs include proteins possessing
hemaglutination activity, such, e.g., a hemagglutinin of
approximately 17-kDa (HA-17), a hemagglutinin of approximately
33-kDa (HA-33) and a hemagglutinin of approximately 70-kDa (HA-70);
as well as non-toxic non-hemagglutinin (NTNH), a protein of
approximately 130-kDa, see, e.g., Eric A. Johnson and Marite
Bradshaw, Clostridial botulinum and its Neurotoxins: A Metabolic
and Cellular Perspective, 39 Toxicon 1703-1722 (2001); and
Stephanie Raffestin et al., Organization and Regulation of the
Neurotoxin Genes in Clostridium botulinum and Clostridium tetani,
10 Anaerobe 93-100 (2004). The toxin complex is important for the
intoxication process because it provides protection from adverse
environmental conditions, resistance to protease digestion, and
appears to facilitate internalization and activation of the
toxin.
[0333] Recent crystallography experiments have revealed that HA-17,
HA-33 and NTNH from various Clostridial bacteria contain a region
comprising .beta.-trefoil domains very similar to the single
.beta.-trefoil domain present in the binding domain of Clostridial
toxins, see, e.g., Kaoru Inoue et al., Structural Analysis by X-Ray
Crystallography and calorimtry of a Haemagglutinin Component (HA1)
of the Progenitor Toxin from Clostridium botulinum, 149 Microbiol.
3361-3370 (2003); and Joseph W. Arndt et al., The Structure of the
Neurotoxin-Associated Protein HA33/A from Clostridial botulinum
Suggests a Reoccurring .beta.-trefoil Fold in the Progenitor Toxin
Complex, 346 J. Mol. Biol. 1083-1093 (2005). For example, HA-33
from Clostridium botulinum serotype A has two .beta.-trefoil
domains, each of which consists of three potential carbohydrate
binding moieties or .beta.-trefoil folds, designated 1.alpha.
(amino acids 10-55 of SEQ ID NO: 9), 111 (amino acids 56-102 of SEQ
ID NO: 9) and 1.gamma. (amino acids 103-144 of SEQ ID NO: 9) for
the first .beta.-trefoil domain and 2.alpha. (amino acids 151-197
of SEQ ID NO: 9), 2.beta. (amino acids 198-245 of SEQ ID NO: 9) and
2.gamma. (amino acids 246-293 of SEQ ID NO: 9) for the second
.beta.-trefoil domain. Mutations in conserved amino acids of the
carbohydrate binding moiety result in a loss of carbohydrate
binding, see, e.g., Kaoru Inoue et al., Structural Analysis by
X-Ray Crystallography and calorimetry of a Haemagglutinin Component
(HA1) of the Progenitor Toxin from Clostridium botulinum, 149
Microbiol. 3361-3370 (2003).
[0334] These .beta.-trefoil domains are also found in the HA-33
proteins produced by Clostridium botulinum serotype B, serotype C1
and serotype D and sequence alignments revealed that amino acids
essential for overall carbohydrate binding are conserved. The amino
acids predicted to be essential for carbohydrate binding are as
follows Asp 263, Tyr 265, Gln 268, Gln 276, Phe 278 and Gln 286 of
HA-33/A1 of SEQ ID NO: 9, HA-33/A2 of SEQ ID NO: 10, HA-33/A3 of
SEQ ID NO: 11, HA-33/A5 of SEQ ID NO: 12 and HA-33/B2 of SEQ ID NO:
15; Asp 264, Tyr 266, Gln 269, Gln 277, Phe 279 and Gln 287 of
HA-33/A4 of SEQ ID NO: 12; Asp 262, Tyr 264, Gln 267, Gln 275, Phe
277 and Gln 285 of HA-33/B1 of SEQ ID NO: 14; Asp 255, Val 257, Gly
260, Gln 268, Typ 270 and Gln 278 of HA-33/C1 of SEQ ID NO: 16; and
Asp 256, Tyr 258, Gln 261, Ile 269, Asp 271 and Gln 279 of HA-33/C2
of SEQ ID NO: 17 and HA-33/D of SEQ ID NO: 18. Immunoaffinity
column chromatography and pull-down assays have shown that HA-33
can bind synaptotagmin II, a putative Clostridial toxin receptor,
see, e.g., Yu Zhou et al., Haemagglutinin-33 of Type Q Botulinum
Neurotoxin Complex Binds with Synaptotagmin II, 272 FEBS Lett.
2717-2726 (2005). The amino acid sequences comprising the
.beta.-trefoil domains found in various Clostridial HA-33 proteins
are shown in Tables 3 and 4.
TABLE-US-00003 TABLE 3 .beta.-trefoil Domains of Clostridial HA-33
Proteins Amino Acid Sequence Region of Carbohydrate Binding
Moieties 1.beta.4/.beta.5 1.beta.8/.beta.9 Protein SEQ ID NO:
1.alpha.-fold .beta.-hairpin turn 1.beta.-fold .beta.-hairpin turn
1.gamma.-fold HA-33/A1 9 10-54 55-59 60-100 101-104 105-144
HA-33/A2 10 10-54 55-59 60-100 101-104 105-144 HA-33/A3 11 10-54
55-59 60-100 101-104 105-144 HA-33/A4 12 10-56 57-61 62-102 103-106
107-146 HA-33/A5 13 10-54 55-59 60-100 101-104 105-144 HA-33/B1 14
10-54 55-59 60-100 101-104 105-144 HA-33/B2 15 10-56 57-61 62-102
103-106 107-146 HA-33/C1-1 16 10-54 55-59 60-98 99-102 103-141
HA-33/C1-2 17 10-54 55-59 60-98 99-102 103-141 HA-33/D1 18 10-54
55-59 60-98 99-102 103-141
TABLE-US-00004 TABLE 4 .beta.-trefoil Domains of Clostridial HA-33
Proteins Amino Acid Sequence Region of Carbohydrate Binding
Moieties 2.beta.4/.beta.5 2.beta.8/.beta.9 Protein SEQ ID NO:
2.alpha.-fold .beta.-hairpin turn 2.beta.-fold .beta.-hairpin turn
2.gamma.-fold HA-33/A1 9 151-195 196-199 200-242 243-248 249-293
HA-33/A2 10 151-195 196-199 200-242 243-248 249-293 HA-33/A3 11
151-195 196-199 200-242 243-248 249-293 HA-33/A4 12 153-197 198-201
202-243 244-249 250-294 HA-33/A5 13 151-195 196-199 200-242 243-248
249-279 HA-33/B1 14 151-195 196-199 200-241 242-247 248-292
HA-33/B2 15 153-197 198-201 200-242 243-248 249-291 HA-33/C1-1 16
148-190 191-194 195-234 235-240 241-285 HA-33/C1-2 17 148-190
191-194 195-235 236-241 242-286 HA-33/D1 18 148-190 191-194 195-235
236-241 242-286
[0335] Further analysis of the .beta.-trefoil domain sequence of
HA-33 also identified .beta.-trefoil domains in HA-17 and NTNH,
see, e.g., Joseph W. Arndt et al., The Structure of the
Neurotoxin-Associated Protein HA33/A from Clostridial botulinum
Suggests a Reoccurring .beta.-trefoil Fold in the Progenitor Toxin
Complex, 346 J. Mol. Biol. 1083-1093 (2005). The HA-17 comprises a
single .beta.-trefoil domain containing three carbohydrate binding
moieties or .beta.-trefoil folds. The carbohydrate binding moieties
of HA-17 exhibits the greatest sequence similarity with the
2.gamma. carbohydrate binding moiety of HA-33. These .beta.-trefoil
domains are also found in the HA-17 proteins produced by
Clostridium botulinum serotype B, serotype C1 and serotype D and
sequence alignments revealed that amino acids essential for overall
carbohydrate binding are conserved. The amino acids predicted to be
essential for carbohydrate binding are as follows Tyr 110, Typ 112,
Tyr 115, Pro 130, Phe 132 and Asn 138 of HA-17/A of SEQ ID NO: 19,
HA-17/B of SEQ ID NO: 20, HA-17/C1 of SEQ ID NO: 21 and HA-17/D of
SEQ ID NO: 22. The amino acid sequences comprising the
.beta.-trefoil domains found in various Clostridial HA-17 proteins
are shown in Table 5.
TABLE-US-00005 TABLE 5 .beta.-trefoil Domains of Clostridial HA-17
Proteins Amino Acid Sequence Region of Carbohydrate Binding
Moieties .beta.4/.beta.5 .beta.8/.beta.9 Protein SEQ ID NO:
.alpha.-fold .beta.-hairpin turn .beta.-fold .beta.-hairpin turn
.gamma.-fold HA-17/A 19 9-50 51-54 55-91 92-94 95-146 HA-17/B 20
9-50 51-54 55-91 92-94 95-146 HA-17/C1 21 9-50 51-54 55-91 92-94
95-146 HA-17/D 22 9-50 51-54 55-91 92-94 95-146
[0336] NTNH from various Clostridial bacteria shows significant
sequence similarity to the .beta.-trefoil domains present in the
cell binding domain of BoNT/A and TeNT. The high degree of
structural similarity is interesting in light of the low sequence
similarity between NTNH and the Clostridial toxins. Furthermore,
since NTNH of the various serotypes have greater sequence
similarity than the Clostridial toxins, it is likely that the NTNH
produced by other Clostridial strains will also have .beta.-trefoil
domains exhibiting high structural similarity with the binding
domains of Clostridial toxins. The .beta.-trefoil domains of
various Clostridial NTNHs are as follows: amino acids 1050-1193 of
NTNH/A1 of SEQ ID NO: 23; amino acids 1050-1198 of NTNH/A2 of SEQ
ID NO: 24; amino acids 1050-1193 of NTNH/A3 of SEQ ID NO: 25; amino
acids 1049-1197 of NTNH/B of SEQ ID NO: 26; amino acids 1049-1196
of NTNH/C1 of SEQ ID NO: 27; amino acids 1049-1196 of NTNH/D of SEQ
ID NO: 28; amino acids 1014-1162 of NTNH/E of SEQ ID NO: 29; amino
acids 1016-1159 of NTNH/F1 of SEQ ID NO: 30; amino acids 1017-1165
of NTNH/F2 of SEQ ID NO: 31; and amino acids 1050-1198 of NTNH/G of
SEQ ID NO: 32. The amino acid sequences comprising the
.beta.-trefoil domains found in various Clostridial NTNH proteins
are shown in Table 6.
[0337] The .beta.-trefoil domains present in the Clostridial toxin,
HA-33, HA-17 and NTNH collectively form nearly half the mass of a
Clostridial toxin complex and underlies the apparent importance of
carbohydrate binding in the cell binding step of the intoxication
process. This observation is further enhanced by the fact that a
Clostridial toxin alone is not as effective in intoxicating a cell
as the entire toxin complex. One potential explanation for this
enhanced binding activity is the presence, both in type and in
quantity, of the .beta.-trefoil domains present in HA-33, HA-17 and
NTNH. Therefore, the high prediction of structural similarity of
the .beta.-trefoil domains present in HA-33, HA-17 and NTNH
relative to the .beta.-trefoil domain found in Clostridial toxins
provides a potential source of binding domains useful for
developing modified Clostridial toxins with enhanced binding
activity. As a non-limiting example, a carbohydrate binding moiety
or a .beta.-trefoil fold from HA-33, HA-17 or NTNH can be
substituted for the naturally occurring carbohydrate binding moiety
present in a Clostridial toxin. As another non-limiting example, a
carbohydrate binding moiety or a .beta.-trefoil fold from HA-33,
HA-17 or NTNH can be added in addition to the naturally occurring
carbohydrate binding moiety present in a Clostridial toxin. As yet
another non-limiting example, a multiple carbohydrate binding
moieties or a .beta.-trefoil folds from HA-33, HA-17 or NTNH can be
substituted for the naturally occurring carbohydrate binding moiety
present in a Clostridial toxin. As still another non-limiting
example, multiple carbohydrate binding moieties or a .beta.-trefoil
folds from HA-33, HA-17 or NTNH can be added in addition to the
naturally occurring carbohydrate binding moiety present in a
Clostridial toxin. As another non-limiting example, multiple
carbohydrate binding moieties or a .beta.-trefoil folds from a
Clostridial toxin binding domain can be added in addition to the
naturally occurring carbohydrate binding moiety present in a
Clostridial toxin.
TABLE-US-00006 TABLE 6 .beta.-trefoil Domains of Clostridial NTNH
Proteins Amino Acid Sequence Region of Carbohydrate Binding
Moieties .beta.4/.beta.5 .beta.8/.beta.9 Protein SEQ ID NO:
.alpha.-fold .beta.-hairpin turn .beta.-fold .beta.-hairpin turn
.gamma.-fold NTNH/A1 23 1050-1097 1098-1110 1111-1138 1139-1148
1149-1194 NTNH/A2 24 1050-1097 1098-1110 1111-1139 1140-1148
1149-1199 NTNH/A3 25 1050-1097 1098-1110 1111-1138 1139-1148
1149-1194 NTNH/B 26 1049-1096 1097-1109 1110-1138 1139-1147
1148-1198 NTNH/C1 27 1049-1096 1097-1109 1110-1138 1139-1147
1148-1197 NTNH/D 28 1049-1096 1097-1109 1110-1138 1139-1147
1148-1197 NTNH/E 29 1014-1061 1062-1074 1075-1103 1104-1113
1114-1163 NTNH/F1 30 1016-1063 1064-1076 1077-1104 1105-1114
1115-1160 NTNH/F2 31 1017-1064 1065-1077 1078-1106 1107-1116
1117-1166 NTNH/G 32 1050-1097 1098-1110 1111-1139 1140-1149
1150-1199
[0338] As used herein, the term "Non-toxin Associated Protein" is
synonymous with "NAP" and means a Clostridial NAP with selective
binding activity, such as, e.g., a binding affinity or a binding
specificity, for an endogenous Clostridial toxin receptor. It is
envisioned that both naturally occurring NAPs as well as NAPs with
enhanced binding activity can be used to practice aspects of the
present invention. As used herein, the term "NAP with enhanced
binding activity" means a Clostridial NAP with enhanced binding
activity for an endogenous Clostridial toxin receptor, such as,
e.g., a binding affinity or a binding specificity, to a
statistically significantly degree relative to an unmodified
naturally occurring Clostridial toxin binding domain from a
Clostridial toxin. By definition, a NAP with enhanced binding
activity has at least one amino acid change from the corresponding
region of the disclosed reference sequences (see Table 3-6) and can
be described in percent identity to the corresponding region of
that reference sequence.
[0339] Any of a variety of sequence alignment methods can be used
to determine percent identity of a modified Clostridial NAP
relative to a naturally-occurring Clostridial NAP, including,
without limitation, global methods, local methods and hybrid
methods, such as, e.g., segment approach methods. Protocols to
determine percent identity are routine procedures within the scope
of one skilled in the art and from the teaching herein.
[0340] Approaches well known to one skilled in the art on how to
modify a Clostridial NAP in order to increase its binding activity
for an endogenous Clostridial toxin receptor present on a
naturally-occurring Clostridial toxin target cell. As described
above, one approach involves identifying amino acids using
computational protein design algorithms; changing
specifically-identified amino acids using, without limitation,
site-directed mutagenesis, oligonucleotide-directed mutagenesis and
site-specific mutagenesis; and testing the binding activity of
modified Clostridial toxins comprising a modified Clostridial NAP
with enhanced binding activity using, e.g., heterogeneous assays,
homogeneous assays and non-separating homogeneous assays. It is
further envisioned that the binding activity of a modified
Clostridial toxin with enhanced binding activity disclosed in the
present specification can be determined by affinity chromatography
using immobilized receptors and interfacial optical assays. In
another approach described above, a binding activity of a modified
Clostridial NAP for a naturally-occurring Clostridial toxin
receptor present on a naturally-occurring Clostridial toxin target
cell can be achieved using directed-evolution methods.
[0341] A Clostridial NAP includes, without limitation, naturally
occurring Clostridial NAP variants, such as, e.g., Clostridial NAP
isoforms and Clostridial NAP subtypes; non-naturally occurring
Clostridial NAP variants, such as, e.g., conservative Clostridial
NAP variants, non-conservative Clostridial NAP variants,
Clostridial NAP chimerics, active Clostridial NAP fragments
thereof, or any combination thereof.
[0342] As used herein, the term "Clostridial NAP variant," whether
naturally-occurring or non-naturally-occurring, means a Clostridial
NAP that has at least one amino acid change from the corresponding
region of the disclosed reference sequences (see Tables 3-6) and
can be described in percent identity to the corresponding region of
that reference sequence. Unless expressly indicated, all
Clostridial NAP variants disclosed in the present specification are
capable of executing the cell binding step of the intoxication
process.
[0343] It is recognized by those of skill in the art that within
each Clostridial bacterium there can be naturally occurring
Clostridial NAP variants that differ somewhat in their amino acid
sequence, and also in the nucleic acids encoding these proteins.
For example, there are presently five Clostridial botulinum
serotype A HA-33 variants, HA-33/A1, HA-33/A2, HA-33/A3, HA-33/A4
and HA-33/A5 (Tables 3 and 4), with specific HA-33 variants showing
various degrees of amino acid divergence when compared to another
HA-33 variant. As another example, there are presently three
Clostridial botulinum serotype A NTNH-33 variants, NTNH/A1, NTNH/A2
and NTNH/A3 (Table 6), with specific NTNH variant showing various
degrees of amino acid divergence when compared to another NTNH
variant. As used herein, the term "naturally occurring Clostridial
NAP variant" means any Clostridial NAP produced by a
naturally-occurring process, including, without limitation,
Clostridial NAP isoforms produced from alternatively-spliced
transcripts, Clostridial NAP isoforms produced by spontaneous
mutation and Clostridial NAP subtypes. A naturally occurring
Clostridial NAP variant can function in substantially the same
manner as the reference Clostridial NAP on which the naturally
occurring Clostridial NAP variant is based, and can be substituted
for the reference Clostridial NAP in any aspect of the present
invention. A naturally occurring Clostridial NAP variant may
substitute one or more amino acids, two or more amino acids, three
or more amino acids, four or more amino acids, five or more amino
acids, ten or more amino acids, 20 or more amino acids, 30 or more
amino acids, 40 or more amino acids, 50 or more amino acids or 100
or more amino acids from the reference Clostridial NAP on which the
naturally occurring Clostridial NAP variant is based. A naturally
occurring Clostridial NAP variant can also substitute at least 10
contiguous amino acids, at least 15 contiguous amino acids, at
least 20 contiguous amino acids, or at least 25 contiguous amino
acids from the reference Clostridial NAP on which the naturally
occurring Clostridial NAP variant is based, that possess at least
50% amino acid identity, 65% amino acid identity, 75% amino acid
identity, 85% amino acid identity or 95% amino acid identity to the
reference Clostridial NAP on which the naturally occurring
Clostridial NAP variant is based.
[0344] A non-limiting examples of a naturally occurring Clostridial
NAP variant is a Clostridial NAP isoform such as, e.g., a
Clostridial botulinum serotype A HA-33 isoform, a Clostridial
botulinum serotype B HA-33 isoform, a Clostridial botulinum
serotype C1 HA-33 isoform, a Clostridial botulinum serotype D HA-33
isoform, a Clostridial botulinum serotype A HA-17 isoform, a
Clostridial botulinum serotype B HA-17 isoform, a Clostridial
botulinum serotype C1 HA-17 isoform, a Clostridial botulinum
serotype D HA-17 isoform, a Clostridial botulinum serotype A NTNH
isoform, a Clostridial botulinum serotype B NTNH isoform, a
Clostridial botulinum serotype C1 NTNH isoform, a Clostridial
botulinum serotype D NTNH isoform, a Clostridial botulinum serotype
E NTNH isoform, a Clostridial botulinum serotype F NTNH isoform and
a Clostridial botulinum serotype G NTNH isoform. A Clostridial NAP
isoform can function in substantially the same manner as the
reference Clostridial NAP on which the Clostridial NAP isoform is
based, and can be substituted for the reference Clostridial NAP in
any aspect of the present invention.
[0345] Another non-limiting examples of a naturally occurring
Clostridial NAP variant is a Clostridial NAP subtype such as, e.g.,
a Clostridial botulinum serotype A HA-33 subtype, a Clostridial
botulinum serotype B HA-33 subtype, a Clostridial botulinum
serotype C1 HA-33 subtype, a Clostridial botulinum serotype D HA-33
subtype, a Clostridial botulinum serotype A HA-17 subtype, a
Clostridial botulinum serotype B HA-17 subtype, a Clostridial
botulinum serotype C1 HA-17 subtype, a Clostridial botulinum
serotype D HA-17 subtype, a Clostridial botulinum serotype A NTNH
subtype, a Clostridial botulinum serotype B NTNH subtype, a
Clostridial botulinum serotype C1 NTNH subtype, a Clostridial
botulinum serotype D NTNH subtype, a Clostridial botulinum serotype
E NTNH subtype, a Clostridial botulinum serotype F NTNH subtype and
a Clostridial botulinum serotype G NTNH subtype. A Clostridial NAP
subtype can function in substantially the same manner as the
reference Clostridial NAP on which the Clostridial NAP subtype is
based, and can be substituted for the reference Clostridial NAP in
any aspect of the present invention.
[0346] As used herein, the term "non-naturally occurring
Clostridial NAP variant" means any Clostridial NAP produced with
the aid of human manipulation, including, without limitation,
Clostridial NAPs produced by genetic engineering using random
mutagenesis or rational design and Clostridial NAPs produced by
chemical synthesis. Non-limiting examples of non-naturally
occurring Clostridial NAP variants include, e.g., conservative
Clostridial NAP variants, non-conservative Clostridial NAP
variants, Clostridial NAP chimeric variants and active Clostridial
NAP fragments.
[0347] As used herein, the term "conservative Clostridial NAP
variant" means a Clostridial NAP that has at least one amino acid
substituted by another amino acid or an amino acid analog that has
at least one property similar to that of the original amino acid
from the reference Clostridial NAP sequence (see Tables 3-6).
Examples of properties include, without limitation, similar size,
topography, charge, hydrophobicity, hydrophilicity, lipophilicity,
covalent-bonding capacity, hydrogen-bonding capacity, a
physicochemical property, of the like, or any combination thereof.
A conservative Clostridial NAP variant can function in
substantially the same manner as the reference Clostridial NAP on
which the conservative Clostridial NAP variant is based, and can be
substituted for the reference Clostridial NAP in any aspect of the
present invention. A conservative Clostridial NAP variant may
substitute one or more amino acids, two or more amino acids, three
or more amino acids, four or more amino acids, five or more amino
acids, ten or more amino acids, 20 or more amino acids, 30 or more
amino acids, 40 or more amino acids or 50 or more amino acids from
the reference Clostridial NAP on which the conservative Clostridial
NAP variant is based. A conservative Clostridial NAP variant can
also substitute at least 10 contiguous amino acids, at least 15
contiguous amino acids, at least 20 contiguous amino acids, or at
least 25 contiguous amino acids from the reference Clostridial NAP
on which the conservative Clostridial NAP variant is based, that
possess at least 50% amino acid identity, 65% amino acid identity,
75% amino acid identity, 85% amino acid identity or 95% amino acid
identity to the reference Clostridial NAP on which the conservative
Clostridial NAP variant is based. Non-limiting examples of a
conservative Clostridial NAP variant include, e.g., a conservative
Clostridial botulinum serotype A HA-33 variant, a conservative
Clostridial botulinum serotype B HA-33 variant, a conservative
Clostridial botulinum serotype C1 HA-33 variant, a conservative
Clostridial botulinum serotype D HA-33 variant, a conservative
Clostridial botulinum serotype A HA-17 variant, a conservative
Clostridial botulinum serotype B HA-17 variant, a conservative
Clostridial botulinum serotype C1 HA-17 variant, a conservative
Clostridial botulinum serotype D HA-17 variant, a conservative
Clostridial botulinum serotype A NTNH variant, a conservative
Clostridial botulinum serotype B NTNH variant, a conservative
Clostridial botulinum serotype C1 NTNH variant, a conservative
Clostridial botulinum serotype D NTNH variant, a conservative
Clostridial botulinum serotype E NTNH variant, a conservative
Clostridial botulinum serotype F NTNH variant and a conservative
Clostridial botulinum serotype G NTNH variant.
[0348] As used herein, the term "non-conservative Clostridial NAP
variant" means a Clostridial NAP in which 1) at least one amino
acid is deleted from the reference Clostridial NAP on which the
non-conservative Clostridial NAP variant is based; 2) at least one
amino acid added to the reference Clostridial NAP on which the
non-conservative Clostridial NAP is based; or 3) at least one amino
acid is substituted by another amino acid or an amino acid analog
that does not share any property similar to that of the original
amino acid from the reference Clostridial NAP sequence (see Tables
3-6). A non-conservative Clostridial NAP variant can function in
substantially the same manner as the reference Clostridial NAP on
which the non-conservative Clostridial NAP variant is based, and
can be substituted for the reference Clostridial NAP in any aspect
of the present invention. A non-conservative Clostridial NAP
variant can delete one or more amino acids, two or more amino
acids, three or more amino acids, four or more amino acids, five or
more amino acids, and ten or more amino acids from the reference
Clostridial NAP on which the non-conservative Clostridial NAP
variant is based. A non-conservative Clostridial NAP variant can
add one or more amino acids, two or more amino acids, three or more
amino acids, four or more amino acids, five or more amino acids,
and ten or more amino acids to the reference Clostridial NAP on
which the non-conservative Clostridial NAP variant is based. A
non-conservative Clostridial NAP variant may substitute one or more
amino acids, two or more amino acids, three or more amino acids,
four or more amino acids, five or more amino acids, ten or more
amino acids, 20 or more amino acids, 30 or more amino acids, 40 or
more amino acids or 50 or more amino acids from the reference
Clostridial NAP on which the non-conservative Clostridial NAP
variant is based. A non-conservative Clostridial NAP variant can
also substitute at least 10 contiguous amino acids, at least 15
contiguous amino acids, at least 20 contiguous amino acids, or at
least 25 contiguous amino acids from the reference Clostridial NAP
on which the non-conservative Clostridial NAP variant is based,
that possess at least 50% amino acid identity, 65% amino acid
identity, 75% amino acid identity, 85% amino acid identity or 95%
amino acid identity to the reference Clostridial NAP on which the
non-conservative Clostridial NAP variant is based. Non-limiting
examples of a non-conservative Clostridial NAP variant include,
e.g., a non-conservative Clostridial botulinum serotype A HA-33
variant, a non-conservative Clostridial botulinum serotype B HA-33
variant, a non-conservative Clostridial botulinum serotype C1 HA-33
variant, a non-conservative Clostridial botulinum serotype D HA-33
variant, a non-conservative Clostridial botulinum serotype A HA-17
variant, a non-conservative Clostridial botulinum serotype B HA-17
variant, a non-conservative Clostridial botulinum serotype C1 HA-17
variant, a non-conservative Clostridial botulinum serotype D HA-17
variant, a non-conservative Clostridial botulinum serotype A NTNH
variant, a non-conservative Clostridial botulinum serotype B NTNH
variant, a non-conservative Clostridial botulinum serotype C1 NTNH
variant, a non-conservative Clostridial botulinum serotype D NTNH
variant, a non-conservative Clostridial botulinum serotype E NTNH
variant, a non-conservative Clostridial botulinum serotype F NTNH
variant and a non-conservative Clostridial botulinum serotype G
NTNH variant.
[0349] As used herein, the term "Clostridial NAP chimeric" means a
polypeptide comprising at least a portion of a Clostridial NAP and
at least a portion of at least one other polypeptide to form an
enhanced targeting domain with at least one property different from
the reference Clostridial NAP (see Tables 3-6), with the proviso
that this Clostridial NAP chimeric can specifically bind to a
Clostridial toxin receptor present in a Clostridial toxin target
cell, and thus participate in executing the overall cellular
mechanism whereby a Clostridial toxin proteolytically cleaves a
substrate.
[0350] As used herein, the term "active Clostridial NAP fragment"
means any of a variety of Clostridial NAP fragments comprising the
enhanced targeting domain can be useful in aspects of the present
invention with the proviso that these NAP fragments can
specifically bind to a Clostridial toxin receptor present in a
Clostridial toxin target cell, and thus participate in executing
the overall cellular mechanism whereby a Clostridial toxin
proteolytically cleaves a substrate.
[0351] Thus, in an embodiment, a modified Clostridial toxin
disclosed in the present specification comprises an enhanced
targeting domain comprising a .beta.-trefoil domain derived from a
NAP. In another embodiment, a modified Clostridial toxin disclosed
in the present specification comprises an enhanced targeting domain
comprising a .beta.-trefoil domain with enhanced binding activity
derived from a NAP.
[0352] In another embodiment, a modified Clostridial toxin
disclosed in the present specification comprises an enhanced
targeting domain comprising a .beta.-trefoil domain derived from a
Clostridial HA-33. In an aspect of this embodiment, a
.beta.-trefoil domain derived from a Clostridial HA-33 comprises,
e.g., a .beta.-trefoil domain derived from a Clostridial botulinum
serotype A HA-33, a .beta.-trefoil domain derived from a
Clostridial botulinum serotype B HA-33, a .beta.-trefoil domain
derived from a Clostridial botulinum serotype C1 HA-33 or a
.beta.-trefoil domain derived from a Clostridial botulinum serotype
D HA-33. In another aspect of this embodiment, a .beta.-trefoil
domain derived from a Clostridial HA-33 comprises a 1.alpha.-fold
motif of a 1.beta.-trefoil domain of a Clostridial botulinum
serotype A HA-33, a 1.beta.-fold motif of a .beta.-trefoil domain
of a Clostridial botulinum serotype A HA-33, a 1.gamma.-fold motif
of a .beta.-trefoil domain of a Clostridial botulinum serotype A
HA-33, a 2.alpha.-fold motif of a .beta.-trefoil domain of a
Clostridial botulinum serotype A HA-33, a 2.beta.-fold motif of a
.beta.-trefoil domain of a Clostridial botulinum serotype A HA-33,
or a 2.gamma.-fold motif of a .beta.-trefoil domain of a
Clostridial botulinum serotype A HA-33.
[0353] In another embodiment, a modified Clostridial toxin
disclosed in the present specification comprises an enhanced
targeting domain comprising a .beta.-trefoil domain with enhanced
binding activity derived from a Clostridial HA-33. In an aspect of
this embodiment, a .beta.-trefoil domain with enhanced binding
activity derived from a Clostridial HA-33 comprises, e.g., a
.beta.-trefoil domain with enhanced binding activity derived from a
Clostridial botulinum serotype A HA-33, a .beta.-trefoil domain
with enhanced binding activity derived from a Clostridial botulinum
serotype B HA-33, a .beta.-trefoil domain with enhanced binding
activity derived from a Clostridial botulinum serotype C1 HA-33 or
a .beta.-trefoil domain with enhanced binding activity derived from
a Clostridial botulinum serotype D HA-33. In another aspect of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a Clostridial HA-33 comprises a modified 1.alpha.-fold
motif of a .beta.-trefoil domain of a Clostridial botulinum
serotype A HA-33, a modified 1.beta.-fold motif of a .beta.-trefoil
domain of a Clostridial botulinum serotype A HA-33, a modified 1
.gamma.-fold motif of a .beta.-trefoil domain of a Clostridial
botulinum serotype A HA-33, a modified 2.alpha.-fold motif of a
.beta.-trefoil domain of a Clostridial botulinum serotype A HA-33,
a modified 2.beta.-fold motif of a .beta.-trefoil domain of a
Clostridial botulinum serotype A HA-33, or a modified 2.gamma.-fold
motif of a .beta.-trefoil domain of a Clostridial botulinum
serotype A HA-33.
[0354] In another embodiment, a .beta.-trefoil domain derived from
a Clostridial botulinum serotype A HA-33 comprises a .beta.-trefoil
domain derived from a Clostridial botulinum serotype A HA-33 of SEQ
ID NO: 9. In another embodiment, a .beta.-trefoil domain derived
from a Clostridial botulinum serotype A HA-33 comprises amino acids
10-144 or amino acids 151-293 of SEQ ID NO: 9. In another aspect of
this embodiment, a .beta.-trefoil domain derived from a Clostridial
botulinum serotype A HA-33 comprises a 1.alpha.-fold motif of a
.beta.-trefoil domain of a Clostridial botulinum serotype A HA-33,
a 1.beta.-fold motif of a .beta.-trefoil domain of a Clostridial
botulinum serotype A HA-33, a 1.gamma.-fold motif of a
.beta.-trefoil domain of a Clostridial botulinum serotype A HA-33,
a 2.alpha.-fold motif of a .beta.-trefoil domain of a Clostridial
botulinum serotype A HA-33, a 2.beta.-fold motif of a
.beta.-trefoil domain of a Clostridial botulinum serotype A HA-33,
or a 2.gamma.-fold motif of a .beta.-trefoil domain of a
Clostridial botulinum serotype A HA-33 of SEQ ID NO: 9. In another
aspect of this embodiment, a .beta.-trefoil domain derived from a
Clostridial botulinum serotype A HA-33 comprises amino acids 10-54,
amino acids 60-100, amino acids 105-144, amino acids 151-195, amino
acids 200-242, or amino acids 249-293 of SEQ ID NO: 9.
[0355] In another embodiment, a .beta.-trefoil domain with enhanced
binding activity derived from a Clostridial botulinum serotype A
HA-33 comprises a .beta.-trefoil domain with enhanced binding
activity derived from a Clostridial botulinum serotype A HA-33 of
SEQ ID NO: 9. In another embodiment, a .beta.-trefoil domain with
enhanced binding activity derived from a Clostridial botulinum
serotype A HA-33 comprises amino acids 10-144 or amino acids
151-293 of SEQ ID NO: 9. In another aspect of this embodiment, a
.beta.-trefoil domain with enhanced binding activity derived from a
Clostridial botulinum serotype A HA-33 comprises a modified
1.alpha.-fold motif of a .beta.-trefoil domain of a Clostridial
botulinum serotype A HA-33, a modified 1.beta.-fold motif of a
.beta.-trefoil domain of a Clostridial botulinum serotype A HA-33,
a modified 1.gamma.-fold motif of a .beta.-trefoil domain of a
Clostridial botulinum serotype A HA-33, a modified 2.alpha.-fold
motif of a .beta.-trefoil domain of a Clostridial botulinum
serotype A HA-33, a modified 2.beta.-fold motif of a .beta.-trefoil
domain of a Clostridial botulinum serotype A HA-33, or a modified
2.gamma.-fold motif of a .beta.-trefoil domain of a Clostridial
botulinum serotype A HA-33 of SEQ ID NO: 9. In another aspect of
this embodiment, a .beta.-trefoil domain with enhanced binding
activity derived from a Clostridial botulinum serotype A HA-33
comprises amino acids 10-54, amino acids 60-100, amino acids
105-144, amino acids 151-195, amino acids 200-242, or amino acids
249-293 of SEQ ID NO: 9.
[0356] In other aspects of this embodiment, a Clostridial botulinum
serotype A HA-33 comprising a .beta.-trefoil domain with enhanced
binding activity comprises a polypeptide having, e.g., at least 70%
amino acid identity with amino acids 10-54, amino acids 60-100,
amino acids 105-144, amino acids 151-195, amino acids 200-242, or
amino acids 249-293 of SEQ ID NO: 9, at least 75% amino acid
identity with amino acids 10-54, amino acids 60-100, amino acids
105-144, amino acids 151-195, amino acids 200-242, or amino acids
249-293 of SEQ ID NO: 9, at least 80% amino acid identity with
amino acids 10-54, amino acids 60-100, amino acids 105-144, amino
acids 151-195, amino acids 200-242, or amino acids 249-293 of SEQ
ID NO: 9, at least 85% amino acid identity with amino acids 10-54,
amino acids 60-100, amino acids 105-144, amino acids 151-195, amino
acids 200-242, or amino acids 249-293 of SEQ ID NO: 9, at least 90%
amino acid identity with amino acids 10-54, amino acids 60-100,
amino acids 105-144, amino acids 151-195, amino acids 200-242, or
amino acids 249-293 of SEQ ID NO: 9 or at least 95% amino acid
identity with amino acids 10-54, amino acids 60-100, amino acids
105-144, amino acids 151-195, amino acids 200-242, or amino acids
249-293 of SEQ ID NO: 9. In yet other aspects of this embodiment, a
Clostridial botulinum serotype A HA-33 comprising a .beta.-trefoil
domain with enhanced binding activity comprises a polypeptide
having, e.g., at most 70% amino acid identity with amino acids
10-54, amino acids 60-100, amino acids 105-144, amino acids
151-195, amino acids 200-242, or amino acids 249-293 of SEQ ID NO:
9, at most 75% amino acid identity with amino acids 10-54, amino
acids 60-100, amino acids 105-144, amino acids 151-195, amino acids
200-242, or amino acids 249-293 of SEQ ID NO: 9, at most 80% amino
acid identity with amino acids 10-54, amino acids 60-100, amino
acids 105-144, amino acids 151-195, amino acids 200-242, or amino
acids 249-293 of SEQ ID NO: 9, at most 85% amino acid identity with
amino acids 10-54, amino acids 60-100, amino acids 105-144, amino
acids 151-195, amino acids 200-242, or amino acids 249-293 of SEQ
ID NO: 9, at most 90% amino acid identity with amino acids 10-54,
amino acids 60-100, amino acids 105-144, amino acids 151-195, amino
acids 200-242, or amino acids 249-293 of SEQ ID NO: 9 or at most
95% amino acid identity with amino acids 10-54, amino acids 60-100,
amino acids 105-144, amino acids 151-195, amino acids 200-242, or
amino acids 249-293 of SEQ ID NO: 9.
[0357] In other aspects of this embodiment, a Clostridial botulinum
serotype A HA-33 comprising a 11-trefoil domain with enhanced
binding activity comprises a polypeptide having, e.g., at most one,
two, three, four, five, six, seven, eight, nine, 10 or 20
non-contiguous amino acid substitutions relative to amino acids
10-54, amino acids 60-100, amino acids 105-144, amino acids
151-195, amino acids 200-242, or amino acids 249-293 of SEQ ID NO:
9. In other aspects of this embodiment, a Clostridial botulinum
serotype A HA-33 comprising a .beta.-trefoil domain with enhanced
binding activity comprises a polypeptide having, e.g., at least
one, two, three, four, five, six, seven, eight, nine, 10 or 20
non-contiguous amino acid substitutions relative to amino acids
10-54, amino acids 60-100, amino acids 105-144, amino acids
151-195, amino acids 200-242, or amino acids 249-293 of SEQ ID NO:
9. In yet other aspects of this embodiment, a Clostridial botulinum
serotype A HA-33 comprising a .beta.-trefoil domain with enhanced
binding activity comprises a polypeptide having, e.g., at most one,
two, three, four, five, six, seven, eight, nine, 10 or 20
non-contiguous amino acid deletions relative to amino acids 10-54,
amino acids 60-100, amino acids 105-144, amino acids 151-195, amino
acids 200-242, or amino acids 249-293 of SEQ ID NO: 9. In other
aspects of this embodiment, a Clostridial botulinum serotype A
HA-33 comprising a .beta.-trefoil domain with enhanced binding
activity comprises a polypeptide having, e.g., at least one, two,
three, four, five, six, seven, eight, nine, 10 or 20 non-contiguous
amino acid deletions relative to amino acids 10-54, amino acids
60-100, amino acids 105-144, amino acids 151-195, amino acids
200-242, or amino acids 249-293 of SEQ ID NO: 9. In still other
aspects of this embodiment, a Clostridial botulinum serotype A
HA-33 comprising a .beta.-trefoil domain with enhanced binding
activity comprises a polypeptide having, e.g., at most one, two,
three, four, five, six, seven, eight, nine, 10 or 20 non-contiguous
amino acid additions relative to amino acids 10-54, amino acids
60-100, amino acids 105-144, amino acids 151-195, amino acids
200-242, or amino acids 249-293 of SEQ ID NO: 9. In other aspects
of this embodiment, a Clostridial botulinum serotype A HA-33
comprising a .beta.-trefoil domain with enhanced binding activity
comprises a polypeptide having, e.g., at least one, two, three,
four, five, six, seven, eight, nine, 10 or 20 non-contiguous amino
acid additions relative to amino acids 10-54, amino acids 60-100,
amino acids 105-144, amino acids 151-195, amino acids 200-242, or
amino acids 249-293 of SEQ ID NO: 9.
[0358] In other aspects of this embodiment, a Clostridial botulinum
serotype A HA-33 comprising a 11-trefoil domain with enhanced
binding activity comprises a polypeptide having, e.g., at most one,
two, three, four, five, six, seven, eight, nine, 10 or 20
contiguous amino acid substitutions relative to amino acids 10-54,
amino acids 60-100, amino acids 105-144, amino acids 151-195, amino
acids 200-242, or amino acids 249-293 of SEQ ID NO: 9. In other
aspects of this embodiment, a Clostridial botulinum serotype A
HA-33 comprising a .beta.-trefoil domain with enhanced binding
activity comprises a polypeptide having, e.g., at least one, two,
three, four, five, six, seven, eight, nine, 10 or 20 contiguous
amino acid substitutions relative to amino acids 10-54, amino acids
60-100, amino acids 105-144, amino acids 151-195, amino acids
200-242, or amino acids 249-293 of SEQ ID NO: 9. In yet other
aspects of this embodiment, a Clostridial botulinum serotype A
HA-33 comprising a .beta.-trefoil domain with enhanced binding
activity comprises a polypeptide having, e.g., at most one, two,
three, four, five, six, seven, eight, nine, 10 or 20 contiguous
amino acid deletions relative to amino acids 10-54, amino acids
60-100, amino acids 105-144, amino acids 151-195, amino acids
200-242, or amino acids 249-293 of SEQ ID NO: 9. In other aspects
of this embodiment, a Clostridial botulinum serotype A HA-33
comprising a .beta.-trefoil domain with enhanced binding activity
comprises a polypeptide having, e.g., at least one, two, three,
four, five, six, seven, eight, nine, 10 or 20 contiguous amino acid
deletions relative to amino acids 10-54, amino acids 60-100, amino
acids 105-144, amino acids 151-195, amino acids 200-242, or amino
acids 249-293 of SEQ ID NO: 9. In still other aspects of this
embodiment, a Clostridial botulinum serotype A HA-33 comprising a
.beta.-trefoil domain with enhanced binding activity comprises a
polypeptide having, e.g., at most one, two, three, four, five, six,
seven, eight, nine, 10 or 20 contiguous amino acid additions
relative to amino acids 10-54, amino acids 60-100, amino acids
105-144, amino acids 151-195, amino acids 200-242, or amino acids
249-293 of SEQ ID NO: 9. In other aspects of this embodiment, a
Clostridial botulinum serotype A HA-33 comprising a .beta.-trefoil
domain with enhanced binding activity comprises a polypeptide
having, e.g., at least one, two, three, four, five, six, seven,
eight, nine, 10 or 20 contiguous amino acid additions relative to
amino acids 10-54, amino acids 60-100, amino acids 105-144, amino
acids 151-195, amino acids 200-242, or amino acids 249-293 of SEQ
ID NO: 9.
[0359] In another embodiment, a .beta.-trefoil domain derived from
a Clostridial botulinum serotype A HA-33 comprises a
1.beta.4/.beta.5 hairpin turn of a .beta.-trefoil domain of a
Clostridial botulinum serotype A HA-33, a 1.beta.8/.beta.9 hairpin
turn of a .beta.-trefoil domain of a Clostridial botulinum serotype
A HA-33, a 2.beta.4/.beta.5 hairpin turn of a .beta.-trefoil domain
of a Clostridial botulinum serotype A HA-33 or a 2.beta.8/.beta.9
hairpin turn of a .beta.-trefoil domain of a Clostridial botulinum
serotype A HA-33 of SEQ ID NO: 9. In another aspect of this
embodiment, a .beta.-trefoil domain derived from a Clostridial
botulinum serotype A HA-33 comprises amino acids 55-59, amino acids
101-104, amino acids 196-199, or amino acids 243-248 of SEQ ID NO:
9.
[0360] In another embodiment, a .beta.-trefoil domain with enhanced
binding activity derived from a Clostridial botulinum serotype A
HA-33 comprises a modified 1.beta.4/.beta.5 hairpin turn of a
.beta.-trefoil domain of a Clostridial botulinum serotype A HA-33,
a modified 1.beta.8/.beta.9 hairpin turn of a .beta.-trefoil domain
of a Clostridial botulinum serotype A HA-33, a modified
2.beta.4/.beta.5 hairpin turn of a .beta.-trefoil domain of a
Clostridial botulinum serotype A HA-33 or a modified
2.beta.8/.beta.9 hairpin turn of a .beta.-trefoil domain of a
Clostridial botulinum serotype A HA-33 of SEQ ID NO: 9. In another
aspect of this embodiment, a .beta.-trefoil domain with enhanced
binding activity derived from a Clostridial botulinum serotype A
HA-33 comprises a modification of amino acids 55-59, amino acids
101-104, amino acids 196-199, or amino acids 243-248 of SEQ ID NO:
9.
[0361] In other aspects of this embodiment, a .beta.-trefoil domain
with enhanced binding activity derived from a Clostridial botulinum
serotype A HA-33 comprises a polypeptide having, e.g., at least 70%
amino acid identity with amino acids 55-59, amino acids 101-104,
amino acids 196-199, or amino acids 243-248 of SEQ ID NO: 9, at
least 75% amino acid identity with amino acids 55-59, amino acids
101-104, amino acids 196-199, or amino acids 243-248 of SEQ ID NO:
9, at least 80% amino acid identity with amino acids 55-59, amino
acids 101-104, amino acids 196-199, or amino acids 243-248 of SEQ
ID NO: 9, at least 85% amino acid identity with amino acids 55-59,
amino acids 101-104, amino acids 196-199, or amino acids 243-248 of
SEQ ID NO: 9, at least 90% amino acid identity with amino acids
55-59, amino acids 101-104, amino acids 196-199, or amino acids
243-248 of SEQ ID NO: 9 or at least 95% amino acid identity with
amino acids 55-59, amino acids 101-104, amino acids 196-199, or
amino acids 243-248 of SEQ ID NO: 9. In yet other aspects of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a Clostridial botulinum serotype A HA-33 comprises a
polypeptide having, e.g., at most 70% amino acid identity with
amino acids 55-59, amino acids 101-104, amino acids 196-199, or
amino acids 243-248 of SEQ ID NO: 9, at most 75% amino acid
identity with amino acids 55-59, amino acids 101-104, amino acids
196-199, or amino acids 243-248 of SEQ ID NO: 9, at most 80% amino
acid identity with amino acids 55-59, amino acids 101-104, amino
acids 196-199, or amino acids 243-248 of SEQ ID NO: 9, at most 85%
amino acid identity with amino acids 55-59, amino acids 101-104,
amino acids 196-199, or amino acids 243-248 of SEQ ID NO: 9, at
most 90% amino acid identity with amino acids 55-59, amino acids
101-104, amino acids 196-199, or amino acids 243-248 of SEQ ID NO:
9 or at most 95% amino acid identity with amino acids 55-59, amino
acids 101-104, amino acids 196-199, or amino acids 243-248 of SEQ
ID NO: 9.
[0362] In other aspects of this embodiment, a .beta.-trefoil domain
with enhanced binding activity derived from a Clostridial botulinum
serotype A HA-33 comprises a polypeptide having, e.g., at most one,
two, three or four non-contiguous amino acid substitutions relative
to amino acids 55-59, amino acids 101-104, amino acids 196-199, or
amino acids 243-248 of SEQ ID NO: 9. In other aspects of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a Clostridial botulinum serotype A HA-33 comprises a
polypeptide having, e.g., at least one, two, three or four
non-contiguous amino acid substitutions relative to amino acids
55-59, amino acids 101-104, amino acids 196-199, or amino acids
243-248 of SEQ ID NO: 9. In other aspects of this embodiment, a
non-contiguous amino acid substitution of any amino acid from amino
acids 55-59, amino acids 101-104, amino acids 196-199, or amino
acids 243-248 of SEQ ID NO: 9 can be replaced with glycine. In
other aspects of this embodiment, a non-contiguous amino acid
substitution of any hydrophobic amino acid from amino acids 55-59,
amino acids 101-104, amino acids 196-199, or amino acids 243-248 of
SEQ ID NO: 9 can be replaced with phenylalanine. In yet other
aspects of this embodiment, a .beta.-trefoil domain with enhanced
binding activity derived from a Clostridial botulinum serotype A
HA-33 comprises a polypeptide having, e.g., at most one, two, three
or four non-contiguous amino acid deletions relative to amino acids
55-59, amino acids 101-104, amino acids 196-199, or amino acids
243-248 of SEQ ID NO: 9. In other aspects of this embodiment, a
.beta.-trefoil domain with enhanced binding activity derived from a
Clostridial botulinum serotype A HA-33 comprises a polypeptide
having, e.g., at least one, two, three or four non-contiguous amino
acid deletions relative to amino acids 55-59, amino acids 101-104,
amino acids 196-199, or amino acids 243-248 of SEQ ID NO: 9. In
still other aspects of this embodiment, a .beta.-trefoil domain
with enhanced binding activity derived from a Clostridial botulinum
serotype A HA-33 comprises a polypeptide having, e.g., at most one,
two, three or four non-contiguous amino acid additions relative to
amino acids 55-59, amino acids 101-104, amino acids 196-199, or
amino acids 243-248 of SEQ ID NO: 9. In other aspects of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a Clostridial botulinum serotype A HA-33 comprises a
polypeptide having, e.g., at least one, two, three or four
non-contiguous amino acid additions relative to amino acids 55-59,
amino acids 101-104, amino acids 196-199, or amino acids 243-248 of
SEQ ID NO: 9.
[0363] In other aspects of this embodiment, a .beta.-trefoil domain
with enhanced binding activity derived from a Clostridial botulinum
serotype A HA-33 comprises a polypeptide having, e.g., at most one,
two, three or four contiguous amino acid substitutions relative to
amino acids 55-59, amino acids 101-104, amino acids 196-199, or
amino acids 243-248 of SEQ ID NO: 9. In other aspects of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a Clostridial botulinum serotype A HA-33 comprises a
polypeptide having, e.g., at least one, two, three or four
contiguous amino acid substitutions relative to amino acids 55-59,
amino acids 101-104, amino acids 196-199, or amino acids 243-248 of
SEQ ID NO: 9. In other aspects of this embodiment, contiguous amino
acid substitutions of amino acids from amino acids 55-59, amino
acids 101-104, amino acids 196-199, or amino acids 243-248 of SEQ
ID NO: 9 can be replaced with glycine. In other aspects of this
embodiment, contiguous amino acid substitutions of hydrophobic
amino acids from amino acids 55-59, amino acids 101-104, amino
acids 196-199, or amino acids 243-248 of SEQ ID NO: 9 can be
replaced with phenylalanine. In yet other aspects of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a Clostridial botulinum serotype A HA-33 comprises a
polypeptide having, e.g., at most one, two, three or four
contiguous amino acid deletions relative to amino acids 55-59,
amino acids 101-104, amino acids 196-199, or amino acids 243-248 of
SEQ ID NO: 9. In other aspects of this embodiment, a .beta.-trefoil
domain with enhanced binding activity derived from a Clostridial
botulinum serotype A HA-33 comprises a polypeptide having, e.g., at
least one, two, three or four contiguous amino acid deletions
relative to amino acids 55-59, amino acids 101-104, amino acids
196-199, or amino acids 243-248 of SEQ ID NO: 9. In still other
aspects of this embodiment, a .beta.-trefoil domain with enhanced
binding activity derived from a Clostridial botulinum serotype A
HA-33 comprises a polypeptide having, e.g., at most one, two, three
or four contiguous amino acid additions relative to amino acids
55-59, amino acids 101-104, amino acids 196-199, or amino acids
243-248 of SEQ ID NO: 9. In other aspects of this embodiment, a
.beta.-trefoil domain with enhanced binding activity derived from a
Clostridial botulinum serotype A HA-33 comprises a polypeptide
having, e.g., at least one, two, three or four contiguous amino
acid additions relative to amino acids 55-59, amino acids 101-104,
amino acids 196-199, or amino acids 243-248 of SEQ ID NO: 9.
[0364] In another embodiment, a .beta.-trefoil domain derived from
a Clostridial botulinum serotype A HA-33 comprises a .beta.-trefoil
domain derived from a Clostridial botulinum serotype A HA-33 of SEQ
ID NO: 10. In another embodiment, a .beta.-trefoil domain derived
from a Clostridial botulinum serotype A HA-33 comprises amino acids
10-144 or amino acids 151-293 of SEQ ID NO: 10. In another aspect
of this embodiment, a .beta.-trefoil domain derived from a
Clostridial botulinum serotype A HA-33 comprises a 1.alpha.-fold
motif of a .beta.-trefoil domain of a Clostridial botulinum
serotype A HA-33, a 1.beta.-fold motif of a .beta.-trefoil domain
of a Clostridial botulinum serotype A HA-33, a 1.gamma.-fold motif
of a .beta.-trefoil domain of a Clostridial botulinum serotype A
HA-33, a 2.alpha.-fold motif of a .beta.-trefoil domain of a
Clostridial botulinum serotype A HA-33, a 2.beta.-fold motif of a
.beta.-trefoil domain of a Clostridial botulinum serotype A HA-33,
or a 2.gamma.-fold motif of a .beta.-trefoil domain of a
Clostridial botulinum serotype A HA-33 of SEQ ID NO: 10. In another
aspect of this embodiment, a .beta.-trefoil domain derived from a
Clostridial botulinum serotype A HA-33 comprises amino acids 10-54,
amino acids 60-100, amino acids 105-144, amino acids 151-195, amino
acids 200-242, or amino acids 249-293 of SEQ ID NO: 10.
[0365] In another embodiment, a .beta.-trefoil domain with enhanced
binding activity derived from a Clostridial botulinum serotype A
HA-33 comprises a .beta.-trefoil domain with enhanced binding
activity derived from a Clostridial botulinum serotype A HA-33 of
SEQ ID NO: 10. In another embodiment, a .beta.-trefoil domain with
enhanced binding activity derived from a Clostridial botulinum
serotype A HA-33 comprises amino acids 10-144 or amino acids
151-293 of SEQ ID NO: 10. In another aspect of this embodiment, a
.beta.-trefoil domain with enhanced binding activity derived from a
Clostridial botulinum serotype A HA-33 comprises a modified
1.alpha.-fold motif of a .beta.-trefoil domain of a Clostridial
botulinum serotype A HA-33, a modified 1.beta.-fold motif of a
.beta.-trefoil domain of a Clostridial botulinum serotype A HA-33,
a modified 1.gamma.-fold motif of a .beta.-trefoil domain of a
Clostridial botulinum serotype A HA-33, a modified 2.alpha.-fold
motif of a .beta.-trefoil domain of a Clostridial botulinum
serotype A HA-33, a modified 2.beta.-fold motif of a .beta.-trefoil
domain of a Clostridial botulinum serotype A HA-33, or a modified
2.gamma.-fold motif of a .beta.-trefoil domain of a Clostridial
botulinum serotype A HA-33 of SEQ ID NO: 10. In another aspect of
this embodiment, a .beta.-trefoil domain with enhanced binding
activity derived from a Clostridial botulinum serotype A HA-33
comprises amino acids 10-54, amino acids 60-100, amino acids
105-144, amino acids 151-195, amino acids 200-242, or amino acids
249-293 of SEQ ID NO: 10.
[0366] In other aspects of this embodiment, a Clostridial botulinum
serotype A HA-33 comprising a .beta.-trefoil domain with enhanced
binding activity comprises a polypeptide having, e.g., at least 70%
amino acid identity with amino acids 10-54, amino acids 60-100,
amino acids 105-144, amino acids 151-195, amino acids 200-242, or
amino acids 249-293 of SEQ ID NO: 10, at least 75% amino acid
identity with amino acids 10-54, amino acids 60-100, amino acids
105-144, amino acids 151-195, amino acids 200-242, or amino acids
249-293 of SEQ ID NO: 10, at least 80% amino acid identity with
amino acids 10-54, amino acids 60-100, amino acids 105-144, amino
acids 151-195, amino acids 200-242, or amino acids 249-293 of SEQ
ID NO: 10, at least 85% amino acid identity with amino acids 10-54,
amino acids 60-100, amino acids 105-144, amino acids 151-195, amino
acids 200-242, or amino acids 249-293 of SEQ ID NO: 10, at least
90% amino acid identity with amino acids 10-54, amino acids 60-100,
amino acids 105-144, amino acids 151-195, amino acids 200-242, or
amino acids 249-293 of SEQ ID NO: 10 or at least 95% amino acid
identity with amino acids 10-54, amino acids 60-100, amino acids
105-144, amino acids 151-195, amino acids 200-242, or amino acids
249-293 of SEQ ID NO: 10. In yet other aspects of this embodiment,
a Clostridial botulinum serotype A HA-33 comprising a
.beta.-trefoil domain with enhanced binding activity comprises a
polypeptide having, e.g., at most 70% amino acid identity with
amino acids 10-54, amino acids 60-100, amino acids 105-144, amino
acids 151-195, amino acids 200-242, or amino acids 249-293 of SEQ
ID NO: 10, at most 75% amino acid identity with amino acids 10-54,
amino acids 60-100, amino acids 105-144, amino acids 151-195, amino
acids 200-242, or amino acids 249-293 of SEQ ID NO: 10, at most 80%
amino acid identity with amino acids 10-54, amino acids 60-100,
amino acids 105-144, amino acids 151-195, amino acids 200-242, or
amino acids 249-293 of SEQ ID NO: 10, at most 85% amino acid
identity with amino acids 10-54, amino acids 60-100, amino acids
105-144, amino acids 151-195, amino acids 200-242, or amino acids
249-293 of SEQ ID NO: 10, at most 90% amino acid identity with
amino acids 10-54, amino acids 60-100, amino acids 105-144, amino
acids 151-195, amino acids 200-242, or amino acids 249-293 of SEQ
ID NO: 10 or at most 95% amino acid identity with amino acids
10-54, amino acids 60-100, amino acids 105-144, amino acids
151-195, amino acids 200-242, or amino acids 249-293 of SEQ ID NO:
10.
[0367] In other aspects of this embodiment, a Clostridial botulinum
serotype A HA-33 comprising a .beta.-trefoil domain with enhanced
binding activity comprises a polypeptide having, e.g., at most one,
two, three, four, five, six, seven, eight, nine, 10 or 20
non-contiguous amino acid substitutions relative to amino acids
10-54, amino acids 60-100, amino acids 105-144, amino acids
151-195, amino acids 200-242, or amino acids 249-293 of SEQ ID NO:
10. In other aspects of this embodiment, a Clostridial botulinum
serotype A HA-33 comprising a .beta.-trefoil domain with enhanced
binding activity comprises a polypeptide having, e.g., at least
one, two, three, four, five, six, seven, eight, nine, 10 or 20
non-contiguous amino acid substitutions relative to amino acids
10-54, amino acids 60-100, amino acids 105-144, amino acids
151-195, amino acids 200-242, or amino acids 249-293 of SEQ ID NO:
10. In yet other aspects of this embodiment, a Clostridial
botulinum serotype A HA-33 comprising a .beta.-trefoil domain with
enhanced binding activity comprises a polypeptide having, e.g., at
most one, two, three, four, five, six, seven, eight, nine, 10 or 20
non-contiguous amino acid deletions relative to amino acids 10-54,
amino acids 60-100, amino acids 105-144, amino acids 151-195, amino
acids 200-242, or amino acids 249-293 of SEQ ID NO: 10. In other
aspects of this embodiment, a Clostridial botulinum serotype A
HA-33 comprising a .beta.-trefoil domain with enhanced binding
activity comprises a polypeptide having, e.g., at least one, two,
three, four, five, six, seven, eight, nine, 10 or 20 non-contiguous
amino acid deletions relative to amino acids 10-54, amino acids
60-100, amino acids 105-144, amino acids 151-195, amino acids
200-242, or amino acids 249-293 of SEQ ID NO: 10. In still other
aspects of this embodiment, a Clostridial botulinum serotype A
HA-33 comprising a .beta.-trefoil domain with enhanced binding
activity comprises a polypeptide having, e.g., at most one, two,
three, four, five, six, seven, eight, nine, 10 or 20 non-contiguous
amino acid additions relative to amino acids 10-54, amino acids
60-100, amino acids 105-144, amino acids 151-195, amino acids
200-242, or amino acids 249-293 of SEQ ID NO: 10. In other aspects
of this embodiment, a Clostridial botulinum serotype A HA-33
comprising a .beta.-trefoil domain with enhanced binding activity
comprises a polypeptide having, e.g., at least one, two, three,
four, five, six, seven, eight, nine, 10 or 20 non-contiguous amino
acid additions relative to amino acids 10-54, amino acids 60-100,
amino acids 105-144, amino acids 151-195, amino acids 200-242, or
amino acids 249-293 of SEQ ID NO: 10.
[0368] In other aspects of this embodiment, a Clostridial botulinum
serotype A HA-33 comprising a .beta.-trefoil domain with enhanced
binding activity comprises a polypeptide having, e.g., at most one,
two, three, four, five, six, seven, eight, nine, 10 or 20
contiguous amino acid substitutions relative to amino acids 10-54,
amino acids 60-100, amino acids 105-144, amino acids 151-195, amino
acids 200-242, or amino acids 249-293 of SEQ ID NO: 10. In other
aspects of this embodiment, a Clostridial botulinum serotype A
HA-33 comprising a .beta.-trefoil domain with enhanced binding
activity comprises a polypeptide having, e.g., at least one, two,
three, four, five, six, seven, eight, nine, 10 or 20 contiguous
amino acid substitutions relative to amino acids 10-54, amino acids
60-100, amino acids 105-144, amino acids 151-195, amino acids
200-242, or amino acids 249-293 of SEQ ID NO: 10. In yet other
aspects of this embodiment, a Clostridial botulinum serotype A
HA-33 comprising a .beta.-trefoil domain with enhanced binding
activity comprises a polypeptide having, e.g., at most one, two,
three, four, five, six, seven, eight, nine, 10 or 20 contiguous
amino acid deletions relative to amino acids 10-54, amino acids
60-100, amino acids 105-144, amino acids 151-195, amino acids
200-242, or amino acids 249-293 of SEQ ID NO: 10. In other aspects
of this embodiment, a Clostridial botulinum serotype A HA-33
comprising a .beta.-trefoil domain with enhanced binding activity
comprises a polypeptide having, e.g., at least one, two, three,
four, five, six, seven, eight, nine, 10 or 20 contiguous amino acid
deletions relative to amino acids 10-54, amino acids 60-100, amino
acids 105-144, amino acids 151-195, amino acids 200-242, or amino
acids 249-293 of SEQ ID NO: 10. In still other aspects of this
embodiment, a Clostridial botulinum serotype A HA-33 comprising a
.beta.-trefoil domain with enhanced binding activity comprises a
polypeptide having, e.g., at most one, two, three, four, five, six,
seven, eight, nine, 10 or 20 contiguous amino acid additions
relative to amino acids 10-54, amino acids 60-100, amino acids
105-144, amino acids 151-195, amino acids 200-242, or amino acids
249-293 of SEQ ID NO: 10. In other aspects of this embodiment, a
Clostridial botulinum serotype A HA-33 comprising a .beta.-trefoil
domain with enhanced binding activity comprises a polypeptide
having, e.g., at least one, two, three, four, five, six, seven,
eight, nine, 10 or 20 contiguous amino acid additions relative to
amino acids 10-54, amino acids 60-100, amino acids 105-144, amino
acids 151-195, amino acids 200-242, or amino acids 249-293 of SEQ
ID NO: 10.
[0369] In another embodiment, a .beta.-trefoil domain derived from
a Clostridial botulinum serotype A HA-33 comprises a
1.beta.4/.beta.5 hairpin turn of a .beta.-trefoil domain of a
Clostridial botulinum serotype A HA-33, a 1.beta.8/.beta.9 hairpin
turn of a .beta.-trefoil domain of a Clostridial botulinum serotype
A HA-33, a 2.beta.4/.beta.5 hairpin turn of a .beta.-trefoil domain
of a Clostridial botulinum serotype A HA-33 or a 2.beta.8/.beta.9
hairpin turn of a .beta.-trefoil domain of a Clostridial botulinum
serotype A HA-33 of SEQ ID NO: 10. In another aspect of this
embodiment, a .beta.-trefoil domain derived from a Clostridial
botulinum serotype A HA-33 comprises amino acids 55-59, amino acids
101-104, amino acids 196-199, or amino acids 243-248 of SEQ ID NO:
10.
[0370] In another embodiment, a .beta.-trefoil domain with enhanced
binding activity derived from a Clostridial botulinum serotype A
HA-33 comprises a modified 1.beta.4/.beta.5 hairpin turn of a
.beta.-trefoil domain of a Clostridial botulinum serotype A HA-33,
a modified 1.beta.8/.beta.9 hairpin turn of a .beta.-trefoil domain
of a Clostridial botulinum serotype A HA-33, a modified
2.beta.4/.beta.5 hairpin turn of a .beta.-trefoil domain of a
Clostridial botulinum serotype A HA-33 or a modified
2.beta.88/.beta.9 hairpin turn of a .beta.-trefoil domain of a
Clostridial botulinum serotype A HA-33 of SEQ ID NO: 10. In another
aspect of this embodiment, a .beta.-trefoil domain with enhanced
binding activity derived from a Clostridial botulinum serotype A
HA-33 comprises a modification of amino acids 55-59, amino acids
101-104, amino acids 196-199, or amino acids 243-248 of SEQ ID NO:
10.
[0371] In other aspects of this embodiment, a .beta.-trefoil domain
with enhanced binding activity derived from a Clostridial botulinum
serotype A HA-33 comprises a polypeptide having, e.g., at least 70%
amino acid identity with amino acids 55-59, amino acids 101-104,
amino acids 196-199, or amino acids 243-248 of SEQ ID NO: 10, at
least 75% amino acid identity with amino acids 55-59, amino acids
101-104, amino acids 196-199, or amino acids 243-248 of SEQ ID NO:
10, at least 80% amino acid identity with amino acids 55-59, amino
acids 101-104, amino acids 196-199, or amino acids 243-248 of SEQ
ID NO: 10, at least 85% amino acid identity with amino acids 55-59,
amino acids 101-104, amino acids 196-199, or amino acids 243-248 of
SEQ ID NO: 10, at least 90% amino acid identity with amino acids
55-59, amino acids 101-104, amino acids 196-199, or amino acids
243-248 of SEQ ID NO: 10 or at least 95% amino acid identity with
amino acids 55-59, amino acids 101-104, amino acids 196-199, or
amino acids 243-248 of SEQ ID NO: 10. In yet other aspects of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a Clostridial botulinum serotype A HA-33 comprises a
polypeptide having, e.g., at most 70% amino acid identity with
amino acids 55-59, amino acids 101-104, amino acids 196-199, or
amino acids 243-248 of SEQ ID NO: 10, at most 75% amino acid
identity with amino acids 55-59, amino acids 101-104, amino acids
196-199, or amino acids 243-248 of SEQ ID NO: 10, at most 80% amino
acid identity with amino acids 55-59, amino acids 101-104, amino
acids 196-199, or amino acids 243-248 of SEQ ID NO: 10, at most 85%
amino acid identity with amino acids 55-59, amino acids 101-104,
amino acids 196-199, or amino acids 243-248 of SEQ ID NO: 10, at
most 90% amino acid identity with amino acids 55-59, amino acids
101-104, amino acids 196-199, or amino acids 243-248 of SEQ ID NO:
10 or at most 95% amino acid identity with amino acids 55-59, amino
acids 101-104, amino acids 196-199, or amino acids 243-248 of SEQ
ID NO: 10.
[0372] In other aspects of this embodiment, a .beta.-trefoil domain
with enhanced binding activity derived from a Clostridial botulinum
serotype A HA-33 comprises a polypeptide having, e.g., at most one,
two, three or four non-contiguous amino acid substitutions relative
to amino acids 55-59, amino acids 101-104, amino acids 196-199, or
amino acids 243-248 of SEQ ID NO: 10. In other aspects of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a Clostridial botulinum serotype A HA-33 comprises a
polypeptide having, e.g., at least one, two, three or four
non-contiguous amino acid substitutions relative to amino acids
55-59, amino acids 101-104, amino acids 196-199, or amino acids
243-248 of SEQ ID NO: 10. In other aspects of this embodiment, a
non-contiguous amino acid substitution of any amino acid from amino
acids 55-59, amino acids 101-104, amino acids 196-199, or amino
acids 243-248 of SEQ ID NO: 10 can be replaced with glycine. In
other aspects of this embodiment, a non-contiguous amino acid
substitution of any hydrophobic amino acid from amino acids 55-59,
amino acids 101-104, amino acids 196-199, or amino acids 243-248 of
SEQ ID NO: 10 can be replaced with phenylalanine. In yet other
aspects of this embodiment, a .beta.-trefoil domain with enhanced
binding activity derived from a Clostridial botulinum serotype A
HA-33 comprises a polypeptide having, e.g., at most one, two, three
or four non-contiguous amino acid deletions relative to amino acids
55-59, amino acids 101-104, amino acids 196-199, or amino acids
243-248 of SEQ ID NO: 10. In other aspects of this embodiment, a
.beta.-trefoil domain with enhanced binding activity derived from a
Clostridial botulinum serotype A HA-33 comprises a polypeptide
having, e.g., at least one, two, three or four non-contiguous amino
acid deletions relative to amino acids 55-59, amino acids 101-104,
amino acids 196-199, or amino acids 243-248 of SEQ ID NO: 10. In
still other aspects of this embodiment, a .beta.-trefoil domain
with enhanced binding activity derived from a Clostridial botulinum
serotype A HA-33 comprises a polypeptide having, e.g., at most one,
two, three or four non-contiguous amino acid additions relative to
amino acids 55-59, amino acids 101-104, amino acids 196-199, or
amino acids 243-248 of SEQ ID NO: 10. In other aspects of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a Clostridial botulinum serotype A HA-33 comprises a
polypeptide having, e.g., at least one, two, three or four
non-contiguous amino acid additions relative to amino acids 55-59,
amino acids 101-104, amino acids 196-199, or amino acids 243-248 of
SEQ ID NO: 10.
[0373] In other aspects of this embodiment, a .beta.-trefoil domain
with enhanced binding activity derived from a Clostridial botulinum
serotype A HA-33 comprises a polypeptide having, e.g., at most one,
two, three or four contiguous amino acid substitutions relative to
amino acids 55-59, amino acids 101-104, amino acids 196-199, or
amino acids 243-248 of SEQ ID NO: 10. In other aspects of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a Clostridial botulinum serotype A HA-33 comprises a
polypeptide having, e.g., at least one, two, three or four
contiguous amino acid substitutions relative to amino acids 55-59,
amino acids 101-104, amino acids 196-199, or amino acids 243-248 of
SEQ ID NO: 10. In other aspects of this embodiment, contiguous
amino acid substitutions of amino acids from amino acids 55-59,
amino acids 101-104, amino acids 196-199, or amino acids 243-248 of
SEQ ID NO: 10 can be replaced with glycine. In other aspects of
this embodiment, contiguous amino acid substitutions of hydrophobic
amino acids from amino acids 55-59, amino acids 101-104, amino
acids 196-199, or amino acids 243-248 of SEQ ID NO: 10 can be
replaced with phenylalanine. In yet other aspects of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a Clostridial botulinum serotype A HA-33 comprises a
polypeptide having, e.g., at most one, two, three or four
contiguous amino acid deletions relative to amino acids 55-59,
amino acids 101-104, amino acids 196-199, or amino acids 243-248 of
SEQ ID NO: 10. In other aspects of this embodiment, a
.beta.-trefoil domain with enhanced binding activity derived from a
Clostridial botulinum serotype A HA-33 comprises a polypeptide
having, e.g., at least one, two, three or four contiguous amino
acid deletions relative to amino acids 55-59, amino acids 101-104,
amino acids 196-199, or amino acids 243-248 of SEQ ID NO: 10. In
still other aspects of this embodiment, a .beta.-trefoil domain
with enhanced binding activity derived from a Clostridial botulinum
serotype A HA-33 comprises a polypeptide having, e.g., at most one,
two, three or four contiguous amino acid additions relative to
amino acids 55-59, amino acids 101-104, amino acids 196-199, or
amino acids 243-248 of SEQ ID NO: 10. In other aspects of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a Clostridial botulinum serotype A HA-33 comprises a
polypeptide having, e.g., at least one, two, three or four
contiguous amino acid additions relative to amino acids 55-59,
amino acids 101-104, amino acids 196-199, or amino acids 243-248 of
SEQ ID NO: 10.
[0374] In another embodiment, a .beta.-trefoil domain derived from
a Clostridial botulinum serotype A HA-33 comprises a .beta.-trefoil
domain derived from a Clostridial botulinum serotype A HA-33 of SEQ
ID NO: 11. In another embodiment, a .beta.-trefoil domain derived
from a Clostridial botulinum serotype A HA-33 comprises amino acids
10-144 or amino acids 151-293 of SEQ ID NO: 11. In another aspect
of this embodiment, a .beta.-trefoil domain derived from a
Clostridial botulinum serotype A HA-33 comprises a 1.alpha.-fold
motif of a .beta.-trefoil domain of a Clostridial botulinum
serotype A HA-33, a 1.beta.-fold motif of a .beta.-trefoil domain
of a Clostridial botulinum serotype A HA-33, a 1.gamma.-fold motif
of a .beta.-trefoil domain of a Clostridial botulinum serotype A
HA-33, a 2.alpha.-fold motif of a .beta.-trefoil domain of a
Clostridial botulinum serotype A HA-33, a 2.beta.-fold motif of a
.beta.-trefoil domain of a Clostridial botulinum serotype A HA-33,
or a 2.gamma.-fold motif of a .beta.-trefoil domain of a
Clostridial botulinum serotype A HA-33 of SEQ ID NO: 11. In another
aspect of this embodiment, a .beta.-trefoil domain derived from a
Clostridial botulinum serotype A HA-33 comprises amino acids 10-54,
amino acids 60-100, amino acids 105-144, amino acids 151-195, amino
acids 200-242, or amino acids 249-293 of SEQ ID NO: 11.
[0375] In another embodiment, a .beta.-trefoil domain with enhanced
binding activity derived from a Clostridial botulinum serotype A
HA-33 comprises a .beta.-trefoil domain with enhanced binding
activity derived from a Clostridial botulinum serotype A HA-33 of
SEQ ID NO: 11. In another embodiment, a .beta.-trefoil domain with
enhanced binding activity derived from a Clostridial botulinum
serotype A HA-33 comprises amino acids 10-144 or amino acids
151-293 of SEQ ID NO: 11. In another aspect of this embodiment, a
.beta.-trefoil domain with enhanced binding activity derived from a
Clostridial botulinum serotype A HA-33 comprises a modified
1.alpha.-fold motif of a .beta.-trefoil domain of a Clostridial
botulinum serotype A HA-33, a modified 1.beta.-fold motif of a
.beta.-trefoil domain of a Clostridial botulinum serotype A HA-33,
a modified 1.gamma.-fold motif of a .beta.-trefoil domain of a
Clostridial botulinum serotype A HA-33, a modified 2.alpha.-fold
motif of a .beta.-trefoil domain of a Clostridial botulinum
serotype A HA-33, a modified 2.beta.-fold motif of a .beta.-trefoil
domain of a Clostridial botulinum serotype A HA-33, or a modified
2.gamma.-fold motif of a .beta.-trefoil domain of a Clostridial
botulinum serotype A HA-33 of SEQ ID NO: 11. In another aspect of
this embodiment, a .beta.-trefoil domain with enhanced binding
activity derived from a Clostridial botulinum serotype A HA-33
comprises amino acids 10-54, amino acids 60-100, amino acids
105-144, amino acids 151-195, amino acids 200-242, or amino acids
249-293 of SEQ ID NO: 11.
[0376] In other aspects of this embodiment, a Clostridial botulinum
serotype A HA-33 comprising a .beta.-trefoil domain with enhanced
binding activity comprises a polypeptide having, e.g., at least 70%
amino acid identity with amino acids 10-54, amino acids 60-100,
amino acids 105-144, amino acids 151-195, amino acids 200-242, or
amino acids 249-293 of SEQ ID NO: 11, at least 75% amino acid
identity with amino acids 10-54, amino acids 60-100, amino acids
105-144, amino acids 151-195, amino acids 200-242, or amino acids
249-293 of SEQ ID NO: 11, at least 80% amino acid identity with
amino acids 10-54, amino acids 60-100, amino acids 105-144, amino
acids 151-195, amino acids 200-242, or amino acids 249-293 of SEQ
ID NO: 11, at least 85% amino acid identity with amino acids 10-54,
amino acids 60-100, amino acids 105-144, amino acids 151-195, amino
acids 200-242, or amino acids 249-293 of SEQ ID NO: 11, at least
90% amino acid identity with amino acids 10-54, amino acids 60-100,
amino acids 105-144, amino acids 151-195, amino acids 200-242, or
amino acids 249-293 of SEQ ID NO: 11 or at least 95% amino acid
identity with amino acids 10-54, amino acids 60-100, amino acids
105-144, amino acids 151-195, amino acids 200-242, or amino acids
249-293 of SEQ ID NO: 11. In yet other aspects of this embodiment,
a Clostridial botulinum serotype A HA-33 comprising a
.beta.-trefoil domain with enhanced binding activity comprises a
polypeptide having, e.g., at most 70% amino acid identity with
amino acids 10-54, amino acids 60-100, amino acids 105-144, amino
acids 151-195, amino acids 200-242, or amino acids 249-293 of SEQ
ID NO: 11, at most 75% amino acid identity with amino acids 10-54,
amino acids 60-100, amino acids 105-144, amino acids 151-195, amino
acids 200-242, or amino acids 249-293 of SEQ ID NO: 11, at most 80%
amino acid identity with amino acids 10-54, amino acids 60-100,
amino acids 105-144, amino acids 151-195, amino acids 200-242, or
amino acids 249-293 of SEQ ID NO: 11, at most 85% amino acid
identity with amino acids 10-54, amino acids 60-100, amino acids
105-144, amino acids 151-195, amino acids 200-242, or amino acids
249-293 of SEQ ID NO: 11, at most 90% amino acid identity with
amino acids 10-54, amino acids 60-100, amino acids 105-144, amino
acids 151-195, amino acids 200-242, or amino acids 249-293 of SEQ
ID NO: 11 or at most 95% amino acid identity with amino acids
10-54, amino acids 60-100, amino acids 105-144, amino acids
151-195, amino acids 200-242, or amino acids 249-293 of SEQ ID NO:
11.
[0377] In other aspects of this embodiment, a Clostridial botulinum
serotype A HA-33 comprising a .beta.-trefoil domain with enhanced
binding activity comprises a polypeptide having, e.g., at most one,
two, three, four, five, six, seven, eight, nine, 10 or 20
non-contiguous amino acid substitutions relative to amino acids
10-54, amino acids 60-100, amino acids 105-144, amino acids
151-195, amino acids 200-242, or amino acids 249-293 of SEQ ID NO:
11. In other aspects of this embodiment, a Clostridial botulinum
serotype A HA-33 comprising a .beta.-trefoil domain with enhanced
binding activity comprises a polypeptide having, e.g., at least
one, two, three, four, five, six, seven, eight, nine, 10 or 20
non-contiguous amino acid substitutions relative to amino acids
10-54, amino acids 60-100, amino acids 105-144, amino acids
151-195, amino acids 200-242, or amino acids 249-293 of SEQ ID NO:
11. In yet other aspects of this embodiment, a Clostridial
botulinum serotype A HA-33 comprising a .beta.-trefoil domain with
enhanced binding activity comprises a polypeptide having, e.g., at
most one, two, three, four, five, six, seven, eight, nine, 10 or 20
non-contiguous amino acid deletions relative to amino acids 10-54,
amino acids 60-100, amino acids 105-144, amino acids 151-195, amino
acids 200-242, or amino acids 249-293 of SEQ ID NO: 11. In other
aspects of this embodiment, a Clostridial botulinum serotype A
HA-33 comprising a .beta.-trefoil domain with enhanced binding
activity comprises a polypeptide having, e.g., at least one, two,
three, four, five, six, seven, eight, nine, 10 or 20 non-contiguous
amino acid deletions relative to amino acids 10-54, amino acids
60-100, amino acids 105-144, amino acids 151-195, amino acids
200-242, or amino acids 249-293 of SEQ ID NO: 11. In still other
aspects of this embodiment, a Clostridial botulinum serotype A
HA-33 comprising a .beta.-trefoil domain with enhanced binding
activity comprises a polypeptide having, e.g., at most one, two,
three, four, five, six, seven, eight, nine, 10 or 20 non-contiguous
amino acid additions relative to amino acids 10-54, amino acids
60-100, amino acids 105-144, amino acids 151-195, amino acids
200-242, or amino acids 249-293 of SEQ ID NO: 11. In other aspects
of this embodiment, a Clostridial botulinum serotype A HA-33
comprising a .beta.-trefoil domain with enhanced binding activity
comprises a polypeptide having, e.g., at least one, two, three,
four, five, six, seven, eight, nine, 10 or 20 non-contiguous amino
acid additions relative to amino acids 10-54, amino acids 60-100,
amino acids 105-144, amino acids 151-195, amino acids 200-242, or
amino acids 249-293 of SEQ ID NO: 11.
[0378] In other aspects of this embodiment, a Clostridial botulinum
serotype A HA-33 comprising a .beta.-trefoil domain with enhanced
binding activity comprises a polypeptide having, e.g., at most one,
two, three, four, five, six, seven, eight, nine, 10 or 20
contiguous amino acid substitutions relative to amino acids 10-54,
amino acids 60-100, amino acids 105-144, amino acids 151-195, amino
acids 200-242, or amino acids 249-293 of SEQ ID NO: 11. In other
aspects of this embodiment, a Clostridial botulinum serotype A
HA-33 comprising a .beta.-trefoil domain with enhanced binding
activity comprises a polypeptide having, e.g., at least one, two,
three, four, five, six, seven, eight, nine, 10 or 20 contiguous
amino acid substitutions relative to amino acids 10-54, amino acids
60-100, amino acids 105-144, amino acids 151-195, amino acids
200-242, or amino acids 249-293 of SEQ ID NO: 11. In yet other
aspects of this embodiment, a Clostridial botulinum serotype A
HA-33 comprising a .beta.-trefoil domain with enhanced binding
activity comprises a polypeptide having, e.g., at most one, two,
three, four, five, six, seven, eight, nine, 10 or 20 contiguous
amino acid deletions relative to amino acids 10-54, amino acids
60-100, amino acids 105-144, amino acids 151-195, amino acids
200-242, or amino acids 249-293 of SEQ ID NO: 11. In other aspects
of this embodiment, a Clostridial botulinum serotype A HA-33
comprising a .beta.-trefoil domain with enhanced binding activity
comprises a polypeptide having, e.g., at least one, two, three,
four, five, six, seven, eight, nine, 10 or 20 contiguous amino acid
deletions relative to amino acids 10-54, amino acids 60-100, amino
acids 105-144, amino acids 151-195, amino acids 200-242, or amino
acids 249-293 of SEQ ID NO: 11. In still other aspects of this
embodiment, a Clostridial botulinum serotype A HA-33 comprising a
.beta.-trefoil domain with enhanced binding activity comprises a
polypeptide having, e.g., at most one, two, three, four, five, six,
seven, eight, nine, 10 or 20 contiguous amino acid additions
relative to amino acids 10-54, amino acids 60-100, amino acids
105-144, amino acids 151-195, amino acids 200-242, or amino acids
249-293 of SEQ ID NO: 11. In other aspects of this embodiment, a
Clostridial botulinum serotype A HA-33 comprising a .beta.-trefoil
domain with enhanced binding activity comprises a polypeptide
having, e.g., at least one, two, three, four, five, six, seven,
eight, nine, 10 or 20 contiguous amino acid additions relative to
amino acids 10-54, amino acids 60-100, amino acids 105-144, amino
acids 151-195, amino acids 200-242, or amino acids 249-293 of SEQ
ID NO: 11.
[0379] In another embodiment, a .beta.-trefoil domain derived from
a Clostridial botulinum serotype A HA-33 comprises a 1134/135
hairpin turn of a .beta.-trefoil domain of a Clostridial botulinum
serotype A HA-33, a 1138/139 hairpin turn of a .beta.-trefoil
domain of a Clostridial botulinum serotype A HA-33, a
2.beta.4/.beta.5 hairpin turn of a .beta.-trefoil domain of a
Clostridial botulinum serotype A HA-33 or a 2.beta.8/.beta.9
hairpin turn of a .beta.-trefoil domain of a Clostridial botulinum
serotype A HA-33 of SEQ ID NO: 11. In another aspect of this
embodiment, a .beta.-trefoil domain derived from a Clostridial
botulinum serotype A HA-33 comprises amino acids 55-59, amino acids
101-104, amino acids 196-199, or amino acids 243-248 of SEQ ID NO:
11.
[0380] In another embodiment, a .beta.-trefoil domain with enhanced
binding activity derived from a Clostridial botulinum serotype A
HA-33 comprises a modified 1.beta.4/.beta.5 hairpin turn of a
.beta.-trefoil domain of a Clostridial botulinum serotype A HA-33,
a modified 1.beta.8/.beta.9 hairpin turn of a .beta.-trefoil domain
of a Clostridial botulinum serotype A HA-33, a modified
2.beta.4/.beta.5 hairpin turn of a .beta.-trefoil domain of a
Clostridial botulinum serotype A HA-33 or a modified
2.beta.8/.beta.9 hairpin turn of a .beta.-trefoil domain of a
Clostridial botulinum serotype A HA-33 of SEQ ID NO: 11. In another
aspect of this embodiment, a .beta.-trefoil domain with enhanced
binding activity derived from a Clostridial botulinum serotype A
HA-33 comprises a modification of amino acids 55-59, amino acids
101-104, amino acids 196-199, or amino acids 243-248 of SEQ ID NO:
11.
[0381] In other aspects of this embodiment, a .beta.-trefoil domain
with enhanced binding activity derived from a Clostridial botulinum
serotype A HA-33 comprises a polypeptide having, e.g., at least 70%
amino acid identity with amino acids 55-59, amino acids 101-104,
amino acids 196-199, or amino acids 243-248 of SEQ ID NO: 11, at
least 75% amino acid identity with amino acids 55-59, amino acids
101-104, amino acids 196-199, or amino acids 243-248 of SEQ ID NO:
11, at least 80% amino acid identity with amino acids 55-59, amino
acids 101-104, amino acids 196-199, or amino acids 243-248 of SEQ
ID NO: 11, at least 85% amino acid identity with amino acids 55-59,
amino acids 101-104, amino acids 196-199, or amino acids 243-248 of
SEQ ID NO: 11, at least 90% amino acid identity with amino acids
55-59, amino acids 101-104, amino acids 196-199, or amino acids
243-248 of SEQ ID NO: 11 or at least 95% amino acid identity with
amino acids 55-59, amino acids 101-104, amino acids 196-199, or
amino acids 243-248 of SEQ ID NO: 11. In yet other aspects of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a Clostridial botulinum serotype A HA-33 comprises a
polypeptide having, e.g., at most 70% amino acid identity with
amino acids 55-59, amino acids 101-104, amino acids 196-199, or
amino acids 243-248 of SEQ ID NO: 11, at most 75% amino acid
identity with amino acids 55-59, amino acids 101-104, amino acids
196-199, or amino acids 243-248 of SEQ ID NO: 11, at most 80% amino
acid identity with amino acids 55-59, amino acids 101-104, amino
acids 196-199, or amino acids 243-248 of SEQ ID NO: 11, at most 85%
amino acid identity with amino acids 55-59, amino acids 101-104,
amino acids 196-199, or amino acids 243-248 of SEQ ID NO: 11, at
most 90% amino acid identity with amino acids 55-59, amino acids
101-104, amino acids 196-199, or amino acids 243-248 of SEQ ID NO:
11 or at most 95% amino acid identity with amino acids 55-59, amino
acids 101-104, amino acids 196-199, or amino acids 243-248 of SEQ
ID NO: 11.
[0382] In other aspects of this embodiment, a .beta.-trefoil domain
with enhanced binding activity derived from a Clostridial botulinum
serotype A HA-33 comprises a polypeptide having, e.g., at most one,
two, three or four non-contiguous amino acid substitutions relative
to amino acids 55-59, amino acids 101-104, amino acids 196-199, or
amino acids 243-248 of SEQ ID NO: 11. In other aspects of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a Clostridial botulinum serotype A HA-33 comprises a
polypeptide having, e.g., at least one, two, three or four
non-contiguous amino acid substitutions relative to amino acids
55-59, amino acids 101-104, amino acids 196-199, or amino acids
243-248 of SEQ ID NO: 11. In other aspects of this embodiment, a
non-contiguous amino acid substitution of any amino acid from amino
acids 55-59, amino acids 101-104, amino acids 196-199, or amino
acids 243-248 of SEQ ID NO: 11 can be replaced with glycine. In
other aspects of this embodiment, a non-contiguous amino acid
substitution of any hydrophobic amino acid from amino acids 55-59,
amino acids 101-104, amino acids 196-199, or amino acids 243-248 of
SEQ ID NO: 11 can be replaced with phenylalanine. In yet other
aspects of this embodiment, a .beta.-trefoil domain with enhanced
binding activity derived from a Clostridial botulinum serotype A
HA-33 comprises a polypeptide having, e.g., at most one, two, three
or four non-contiguous amino acid deletions relative to amino acids
55-59, amino acids 101-104, amino acids 196-199, or amino acids
243-248 of SEQ ID NO: 11. In other aspects of this embodiment, a
.beta.-trefoil domain with enhanced binding activity derived from a
Clostridial botulinum serotype A HA-33 comprises a polypeptide
having, e.g., at least one, two, three or four non-contiguous amino
acid deletions relative to amino acids 55-59, amino acids 101-104,
amino acids 196-199, or amino acids 243-248 of SEQ ID NO: 11. In
still other aspects of this embodiment, a .beta.-trefoil domain
with enhanced binding activity derived from a Clostridial botulinum
serotype A HA-33 comprises a polypeptide having, e.g., at most one,
two, three or four non-contiguous amino acid additions relative to
amino acids 55-59, amino acids 101-104, amino acids 196-199, or
amino acids 243-248 of SEQ ID NO: 11. In other aspects of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a Clostridial botulinum serotype A HA-33 comprises a
polypeptide having, e.g., at least one, two, three or four
non-contiguous amino acid additions relative to amino acids 55-59,
amino acids 101-104, amino acids 196-199, or amino acids 243-248 of
SEQ ID NO: 11.
[0383] In other aspects of this embodiment, a .beta.-trefoil domain
with enhanced binding activity derived from a Clostridial botulinum
serotype A HA-33 comprises a polypeptide having, e.g., at most one,
two, three or four contiguous amino acid substitutions relative to
amino acids 55-59, amino acids 101-104, amino acids 196-199, or
amino acids 243-248 of SEQ ID NO: 11. In other aspects of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a Clostridial botulinum serotype A HA-33 comprises a
polypeptide having, e.g., at least one, two, three or four
contiguous amino acid substitutions relative to amino acids 55-59,
amino acids 101-104, amino acids 196-199, or amino acids 243-248 of
SEQ ID NO: 11. In other aspects of this embodiment, contiguous
amino acid substitutions of amino acids from amino acids 55-59,
amino acids 101-104, amino acids 196-199, or amino acids 243-248 of
SEQ ID NO: 11 can be replaced with glycine. In other aspects of
this embodiment, contiguous amino acid substitutions of hydrophobic
amino acids from amino acids 55-59, amino acids 101-104, amino
acids 196-199, or amino acids 243-248 of SEQ ID NO: 11 can be
replaced with phenylalanine. In yet other aspects of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a Clostridial botulinum serotype A HA-33 comprises a
polypeptide having, e.g., at most one, two, three or four
contiguous amino acid deletions relative to amino acids 55-59,
amino acids 101-104, amino acids 196-199, or amino acids 243-248 of
SEQ ID NO: 11. In other aspects of this embodiment, a
.beta.-trefoil domain with enhanced binding activity derived from a
Clostridial botulinum serotype A HA-33 comprises a polypeptide
having, e.g., at least one, two, three or four contiguous amino
acid deletions relative to amino acids 55-59, amino acids 101-104,
amino acids 196-199, or amino acids 243-248 of SEQ ID NO: 11. In
still other aspects of this embodiment, a .beta.-trefoil domain
with enhanced binding activity derived from a Clostridial botulinum
serotype A HA-33 comprises a polypeptide having, e.g., at most one,
two, three or four contiguous amino acid additions relative to
amino acids 55-59, amino acids 101-104, amino acids 196-199, or
amino acids 243-248 of SEQ ID NO: 11. In other aspects of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a Clostridial botulinum serotype A HA-33 comprises a
polypeptide having, e.g., at least one, two, three or four
contiguous amino acid additions relative to amino acids 55-59,
amino acids 101-104, amino acids 196-199, or amino acids 243-248 of
SEQ ID NO: 11.
[0384] In another embodiment, a .beta.-trefoil domain derived from
a Clostridial botulinum serotype A HA-33 comprises a .beta.-trefoil
domain derived from a Clostridial botulinum serotype A HA-33 of SEQ
ID NO: 12. In another embodiment, a .beta.-trefoil domain derived
from a Clostridial botulinum serotype A HA-33 comprises amino acids
10-146 or amino acids 153-294 of SEQ ID NO: 12. In another aspect
of this embodiment, a .beta.-trefoil domain derived from a
Clostridial botulinum serotype A HA-33 comprises a 1.alpha.-fold
motif of a .beta.-trefoil domain of a Clostridial botulinum
serotype A HA-33, a 1.beta.-fold motif of a .beta.-trefoil domain
of a Clostridial botulinum serotype A HA-33, a 1.gamma.-fold motif
of a .beta.-trefoil domain of a Clostridial botulinum serotype A
HA-33, a 2.alpha.-fold motif of a .beta.-trefoil domain of a
Clostridial botulinum serotype A HA-33, a 2.beta.-fold motif of a
.beta.-trefoil domain of a Clostridial botulinum serotype A HA-33,
or a 2.gamma.-fold motif of a .beta.-trefoil domain of a
Clostridial botulinum serotype A HA-33 of SEQ ID NO: 12. In another
aspect of this embodiment, a .beta.-trefoil domain derived from a
Clostridial botulinum serotype A HA-33 comprises amino acids 10-56,
amino acids 62-102, amino acids 107-146, amino acids 153-197, amino
acids 202-243, or amino acids 250-294 of SEQ ID NO: 12.
[0385] In another embodiment, a .beta.-trefoil domain with enhanced
binding activity derived from a Clostridial botulinum serotype A
HA-33 comprises a .beta.-trefoil domain with enhanced binding
activity derived from a Clostridial botulinum serotype A HA-33 of
SEQ ID NO: 12. In another embodiment, a .beta.-trefoil domain with
enhanced binding activity derived from a Clostridial botulinum
serotype A HA-33 comprises amino acids 10-146 or amino acids
153-294 of SEQ ID NO: 12. In another aspect of this embodiment, a
.beta.-trefoil domain with enhanced binding activity derived from a
Clostridial botulinum serotype A HA-33 comprises a modified
1.alpha.-fold motif of a .beta.-trefoil domain of a Clostridial
botulinum serotype A HA-33, a modified 1.beta.-fold motif of a
.beta.-trefoil domain of a Clostridial botulinum serotype A HA-33,
a modified 1.gamma.-fold motif of a .beta.-trefoil domain of a
Clostridial botulinum serotype A HA-33, a modified 2.alpha.-fold
motif of a .beta.-trefoil domain of a Clostridial botulinum
serotype A HA-33, a modified 2.beta.-fold motif of a .beta.-trefoil
domain of a Clostridial botulinum serotype A HA-33, or a modified
2.gamma.-fold motif of a .beta.-trefoil domain of a Clostridial
botulinum serotype A HA-33 of SEQ ID NO: 12. In another aspect of
this embodiment, a .beta.-trefoil domain with enhanced binding
activity derived from a Clostridial botulinum serotype A HA-33
comprises amino acids 10-56, amino acids 62-102, amino acids
107-146, amino acids 153-197, amino acids 202-243, or amino acids
250-294 of SEQ ID NO: 12.
[0386] In other aspects of this embodiment, a Clostridial botulinum
serotype A HA-33 comprising a .beta.-trefoil domain with enhanced
binding activity comprises a polypeptide having, e.g., at least 70%
amino acid identity with amino acids 10-56, amino acids 62-102,
amino acids 107-146, amino acids 153-197, amino acids 202-243, or
amino acids 250-294 of SEQ ID NO: 12, at least 75% amino acid
identity with amino acids 10-56, amino acids 62-102, amino acids
107-146, amino acids 153-197, amino acids 202-243, or amino acids
250-294 of SEQ ID NO: 12, at least 80% amino acid identity with
amino acids 10-56, amino acids 62-102, amino acids 107-146, amino
acids 153-197, amino acids 202-243, or amino acids 250-294 of SEQ
ID NO: 12, at least 85% amino acid identity with amino acids 10-56,
amino acids 62-102, amino acids 107-146, amino acids 153-197, amino
acids 202-243, or amino acids 250-294 of SEQ ID NO: 12, at least
90% amino acid identity with amino acids 10-56, amino acids 62-102,
amino acids 107-146, amino acids 153-197, amino acids 202-243, or
amino acids 250-294 of SEQ ID NO: 12 or at least 95% amino acid
identity with amino acids 10-56, amino acids 62-102, amino acids
107-146, amino acids 153-197, amino acids 202-243, or amino acids
250-294 of SEQ ID NO: 12. In yet other aspects of this embodiment,
a Clostridial botulinum serotype A HA-33 comprising a
.beta.-trefoil domain with enhanced binding activity comprises a
polypeptide having, e.g., at most 70% amino acid identity with
amino acids 10-56, amino acids 62-102, amino acids 107-146, amino
acids 153-197, amino acids 202-243, or amino acids 250-294 of SEQ
ID NO: 12, at most 75% amino acid identity with amino acids 10-56,
amino acids 62-102, amino acids 107-146, amino acids 153-197, amino
acids 202-243, or amino acids 250-294 of SEQ ID NO: 12, at most 80%
amino acid identity with amino acids 10-56, amino acids 62-102,
amino acids 107-146, amino acids 153-197, amino acids 202-243, or
amino acids 250-294 of SEQ ID NO: 12, at most 85% amino acid
identity with amino acids 10-56, amino acids 62-102, amino acids
107-146, amino acids 153-197, amino acids 202-243, or amino acids
250-294 of SEQ ID NO: 12, at most 90% amino acid identity with
amino acids 10-56, amino acids 62-102, amino acids 107-146, amino
acids 153-197, amino acids 202-243, or amino acids 250-294 of SEQ
ID NO: 12 or at most 95% amino acid identity with amino acids
10-56, amino acids 62-102, amino acids 107-146, amino acids
153-197, amino acids 202-243, or amino acids 250-294 of SEQ ID NO:
12.
[0387] In other aspects of this embodiment, a Clostridial botulinum
serotype A HA-33 comprising a 11-trefoil domain with enhanced
binding activity comprises a polypeptide having, e.g., at most one,
two, three, four, five, six, seven, eight, nine, 10 or 20
non-contiguous amino acid substitutions relative to amino acids
10-56, amino acids 62-102, amino acids 107-146, amino acids
153-197, amino acids 202-243, or amino acids 250-294 of SEQ ID NO:
12. In other aspects of this embodiment, a Clostridial botulinum
serotype A HA-33 comprising a .beta.-trefoil domain with enhanced
binding activity comprises a polypeptide having, e.g., at least
one, two, three, four, five, six, seven, eight, nine, 10 or 20
non-contiguous amino acid substitutions relative to amino acids
10-56, amino acids 62-102, amino acids 107-146, amino acids
153-197, amino acids 202-243, or amino acids 250-294 of SEQ ID NO:
12. In yet other aspects of this embodiment, a Clostridial
botulinum serotype A HA-33 comprising a .beta.-trefoil domain with
enhanced binding activity comprises a polypeptide having, e.g., at
most one, two, three, four, five, six, seven, eight, nine, 10 or 20
non-contiguous amino acid deletions relative to amino acids 10-56,
amino acids 62-102, amino acids 107-146, amino acids 153-197, amino
acids 202-243, or amino acids 250-294 of SEQ ID NO: 12. In other
aspects of this embodiment, a Clostridial botulinum serotype A
HA-33 comprising a .beta.-trefoil domain with enhanced binding
activity comprises a polypeptide having, e.g., at least one, two,
three, four, five, six, seven, eight, nine, 10 or 20 non-contiguous
amino acid deletions relative to amino acids 10-56, amino acids
62-102, amino acids 107-146, amino acids 153-197, amino acids
202-243, or amino acids 250-294 of SEQ ID NO: 12. In still other
aspects of this embodiment, a Clostridial botulinum serotype A
HA-33 comprising a .beta.-trefoil domain with enhanced binding
activity comprises a polypeptide having, e.g., at most one, two,
three, four, five, six, seven, eight, nine, 10 or 20 non-contiguous
amino acid additions relative to amino acids 10-56, amino acids
62-102, amino acids 107-146, amino acids 153-197, amino acids
202-243, or amino acids 250-294 of SEQ ID NO: 12. In other aspects
of this embodiment, a Clostridial botulinum serotype A HA-33
comprising a .beta.-trefoil domain with enhanced binding activity
comprises a polypeptide having, e.g., at least one, two, three,
four, five, six, seven, eight, nine, 10 or 20 non-contiguous amino
acid additions relative to amino acids 10-56, amino acids 62-102,
amino acids 107-146, amino acids 153-197, amino acids 202-243, or
amino acids 250-294 of SEQ ID NO: 12.
[0388] In other aspects of this embodiment, a Clostridial botulinum
serotype A HA-33 comprising a .beta.-trefoil domain with enhanced
binding activity comprises a polypeptide having, e.g., at most one,
two, three, four, five, six, seven, eight, nine, 10 or 20
contiguous amino acid substitutions relative to amino acids 10-56,
amino acids 62-102, amino acids 107-146, amino acids 153-197, amino
acids 202-243, or amino acids 250-294 of SEQ ID NO: 12. In other
aspects of this embodiment, a Clostridial botulinum serotype A
HA-33 comprising a .beta.-trefoil domain with enhanced binding
activity comprises a polypeptide having, e.g., at least one, two,
three, four, five, six, seven, eight, nine, 10 or 20 contiguous
amino acid substitutions relative to amino acids 10-56, amino acids
62-102, amino acids 107-146, amino acids 153-197, amino acids
202-243, or amino acids 250-294 of SEQ ID NO: 12. In yet other
aspects of this embodiment, a Clostridial botulinum serotype A
HA-33 comprising a .beta.-trefoil domain with enhanced binding
activity comprises a polypeptide having, e.g., at most one, two,
three, four, five, six, seven, eight, nine, 10 or 20 contiguous
amino acid deletions relative to amino acids 10-56, amino acids
62-102, amino acids 107-146, amino acids 153-197, amino acids
202-243, or amino acids 250-294 of SEQ ID NO: 12. In other aspects
of this embodiment, a Clostridial botulinum serotype A HA-33
comprising a .beta.-trefoil domain with enhanced binding activity
comprises a polypeptide having, e.g., at least one, two, three,
four, five, six, seven, eight, nine, 10 or 20 contiguous amino acid
deletions relative to amino acids 10-56, amino acids 62-102, amino
acids 107-146, amino acids 153-197, amino acids 202-243, or amino
acids 250-294 of SEQ ID NO: 12. In still other aspects of this
embodiment, a Clostridial botulinum serotype A HA-33 comprising a
.beta.-trefoil domain with enhanced binding activity comprises a
polypeptide having, e.g., at most one, two, three, four, five, six,
seven, eight, nine, 10 or 20 contiguous amino acid additions
relative to amino acids 10-56, amino acids 62-102, amino acids
107-146, amino acids 153-197, amino acids 202-243, or amino acids
250-294 of SEQ ID NO: 12. In other aspects of this embodiment, a
Clostridial botulinum serotype A HA-33 comprising a .beta.-trefoil
domain with enhanced binding activity comprises a polypeptide
having, e.g., at least one, two, three, four, five, six, seven,
eight, nine, 10 or 20 contiguous amino acid additions relative to
amino acids 10-56, amino acids 62-102, amino acids 107-146, amino
acids 153-197, amino acids 202-243, or amino acids 250-294 of SEQ
ID NO: 12.
[0389] In another embodiment, a .beta.-trefoil domain derived from
a Clostridial botulinum serotype A HA-33 comprises a
1.beta.4/.beta.5 hairpin turn of a .beta.-trefoil domain of a
Clostridial botulinum serotype A HA-33, a 1.beta.8/.beta.9 hairpin
turn of a .beta.-trefoil domain of a Clostridial botulinum serotype
A HA-33, a 2.beta.4/.beta.5 hairpin turn of a .beta.-trefoil domain
of a Clostridial botulinum serotype A HA-33 or a 2.beta.88/.beta.9
hairpin turn of a .beta.-trefoil domain of a Clostridial botulinum
serotype A HA-33 of SEQ ID NO: 12. In another aspect of this
embodiment, a .beta.-trefoil domain derived from a Clostridial
botulinum serotype A HA-33 comprises amino acids 57-61, amino acids
103-106, amino acids 198-201, or amino acids 244-249 of SEQ ID NO:
12.
[0390] In another embodiment, a .beta.-trefoil domain with enhanced
binding activity derived from a Clostridial botulinum serotype A
HA-33 comprises a modified 1.beta.4/.beta.5 hairpin turn of a
.beta.-trefoil domain of a Clostridial botulinum serotype A HA-33,
a modified 1.beta.8/.beta.9 hairpin turn of a .beta.-trefoil domain
of a Clostridial botulinum serotype A HA-33, a modified
2.beta.4/.beta.5 hairpin turn of a .beta.-trefoil domain of a
Clostridial botulinum serotype A HA-33 or a modified
2.beta.88/.beta.9 hairpin turn of a .beta.-trefoil domain of a
Clostridial botulinum serotype A HA-33 of SEQ ID NO: 12. In another
aspect of this embodiment, a .beta.-trefoil domain with enhanced
binding activity derived from a Clostridial botulinum serotype A
HA-33 comprises a modification of amino acids 57-61, amino acids
103-106, amino acids 198-201, or amino acids 244-249 of SEQ ID NO:
12.
[0391] In other aspects of this embodiment, a .beta.-trefoil domain
with enhanced binding activity derived from a Clostridial botulinum
serotype A HA-33 comprises a polypeptide having, e.g., at least 70%
amino acid identity with amino acids 57-61, amino acids 103-106,
amino acids 198-201, or amino acids 244-249 of SEQ ID NO: 12, at
least 75% amino acid identity with amino acids 57-61, amino acids
103-106, amino acids 198-201, or amino acids 244-249 of SEQ ID NO:
12, at least 80% amino acid identity with amino acids 57-61, amino
acids 103-106, amino acids 198-201, or amino acids 244-249 of SEQ
ID NO: 12, at least 85% amino acid identity with amino acids 57-61,
amino acids 103-106, amino acids 198-201, or amino acids 244-249 of
SEQ ID NO: 12, at least 90% amino acid identity with amino acids
57-61, amino acids 103-106, amino acids 198-201, or amino acids
244-249 of SEQ ID NO: 12 or at least 95% amino acid identity with
amino acids 57-61, amino acids 103-106, amino acids 198-201, or
amino acids 244-249 of SEQ ID NO: 12. In yet other aspects of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a Clostridial botulinum serotype A HA-33 comprises a
polypeptide having, e.g., at most 70% amino acid identity with
amino acids 57-61, amino acids 103-106, amino acids 198-201, or
amino acids 244-249 of SEQ ID NO: 12, at most 75% amino acid
identity with amino acids 57-61, amino acids 103-106, amino acids
198-201, or amino acids 244-249 of SEQ ID NO: 12, at most 80% amino
acid identity with amino acids 57-61, amino acids 103-106, amino
acids 198-201, or amino acids 244-249 of SEQ ID NO: 12, at most 85%
amino acid identity with amino acids 57-61, amino acids 103-106,
amino acids 198-201, or amino acids 244-249 of SEQ ID NO: 12, at
most 90% amino acid identity with amino acids 57-61, amino acids
103-106, amino acids 198-201, or amino acids 244-249 of SEQ ID NO:
12 or at most 95% amino acid identity with amino acids 57-61, amino
acids 103-106, amino acids 198-201, or amino acids 244-249 of SEQ
ID NO: 12.
[0392] In other aspects of this embodiment, a .beta.-trefoil domain
with enhanced binding activity derived from a Clostridial botulinum
serotype A HA-33 comprises a polypeptide having, e.g., at most one,
two, three or four non-contiguous amino acid substitutions relative
to amino acids 57-61, amino acids 103-106, amino acids 198-201, or
amino acids 244-249 of SEQ ID NO: 12. In other aspects of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a Clostridial botulinum serotype A HA-33 comprises a
polypeptide having, e.g., at least one, two, three or four
non-contiguous amino acid substitutions relative to amino acids
57-61, amino acids 103-106, amino acids 198-201, or amino acids
244-249 of SEQ ID NO: 12. In other aspects of this embodiment, a
non-contiguous amino acid substitution of any amino acid from amino
acids 57-61, amino acids 103-106, amino acids 198-201, or amino
acids 244-249 of SEQ ID NO: 12 can be replaced with glycine. In
other aspects of this embodiment, a non-contiguous amino acid
substitution of any hydrophobic amino acid from amino acids 57-61,
amino acids 103-106, amino acids 198-201, or amino acids 244-249 of
SEQ ID NO: 12 can be replaced with phenylalanine. In yet other
aspects of this embodiment, a .beta.-trefoil domain with enhanced
binding activity derived from a Clostridial botulinum serotype A
HA-33 comprises a polypeptide having, e.g., at most one, two, three
or four non-contiguous amino acid deletions relative to amino acids
57-61, amino acids 103-106, amino acids 198-201, or amino acids
244-249 of SEQ ID NO: 12. In other aspects of this embodiment, a
.beta.-trefoil domain with enhanced binding activity derived from a
Clostridial botulinum serotype A HA-33 comprises a polypeptide
having, e.g., at least one, two, three or four non-contiguous amino
acid deletions relative to amino acids 57-61, amino acids 103-106,
amino acids 198-201, or amino acids 244-249 of SEQ ID NO: 12. In
still other aspects of this embodiment, a .beta.-trefoil domain
with enhanced binding activity derived from a Clostridial botulinum
serotype A HA-33 comprises a polypeptide having, e.g., at most one,
two, three or four non-contiguous amino acid additions relative to
amino acids 57-61, amino acids 103-106, amino acids 198-201, or
amino acids 244-249 of SEQ ID NO: 12. In other aspects of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a Clostridial botulinum serotype A HA-33 comprises a
polypeptide having, e.g., at least one, two, three or four
non-contiguous amino acid additions relative to amino acids 57-61,
amino acids 103-106, amino acids 198-201, or amino acids 244-249 of
SEQ ID NO: 12.
[0393] In other aspects of this embodiment, a .beta.-trefoil domain
with enhanced binding activity derived from a Clostridial botulinum
serotype A HA-33 comprises a polypeptide having, e.g., at most one,
two, three or four contiguous amino acid substitutions relative to
amino acids 57-61, amino acids 103-106, amino acids 198-201, or
amino acids 244-249 of SEQ ID NO: 12. In other aspects of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a Clostridial botulinum serotype A HA-33 comprises a
polypeptide having, e.g., at least one, two, three or four
contiguous amino acid substitutions relative to amino acids 57-61,
amino acids 103-106, amino acids 198-201, or amino acids 244-249 of
SEQ ID NO: 12. In other aspects of this embodiment, contiguous
amino acid substitutions of amino acids from amino acids 57-61,
amino acids 103-106, amino acids 198-201, or amino acids 244-249 of
SEQ ID NO: 12 can be replaced with glycine. In other aspects of
this embodiment, contiguous amino acid substitutions of hydrophobic
amino acids from amino acids 57-61, amino acids 103-106, amino
acids 198-201, or amino acids 244-249 of SEQ ID NO: 12 can be
replaced with phenylalanine. In yet other aspects of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a Clostridial botulinum serotype A HA-33 comprises a
polypeptide having, e.g., at most one, two, three or four
contiguous amino acid deletions relative to amino acids 57-61,
amino acids 103-106, amino acids 198-201, or amino acids 244-249 of
SEQ ID NO: 12. In other aspects of this embodiment, a
.beta.-trefoil domain with enhanced binding activity derived from a
Clostridial botulinum serotype A HA-33 comprises a polypeptide
having, e.g., at least one, two, three or four contiguous amino
acid deletions relative to amino acids 57-61, amino acids 103-106,
amino acids 198-201, or amino acids 244-249 of SEQ ID NO: 12. In
still other aspects of this embodiment, a .beta.-trefoil domain
with enhanced binding activity derived from a Clostridial botulinum
serotype A HA-33 comprises a polypeptide having, e.g., at most one,
two, three or four contiguous amino acid additions relative to
amino acids 57-61, amino acids 103-106, amino acids 198-201, or
amino acids 244-249 of SEQ ID NO: 12. In other aspects of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a Clostridial botulinum serotype A HA-33 comprises a
polypeptide having, e.g., at least one, two, three or four
contiguous amino acid additions relative to amino acids 57-61,
amino acids 103-106, amino acids 198-201, or amino acids 244-249 of
SEQ ID NO: 12.
[0394] In another embodiment, a .beta.-trefoil domain derived from
a Clostridial botulinum serotype A HA-33 comprises a .beta.-trefoil
domain derived from a Clostridial botulinum serotype A HA-33 of SEQ
ID NO: 13. In another embodiment, a .beta.-trefoil domain derived
from a Clostridial botulinum serotype A HA-33 comprises amino acids
10-144 or amino acids 151-293 of SEQ ID NO: 13. In another aspect
of this embodiment, a .beta.-trefoil domain derived from a
Clostridial botulinum serotype A HA-33 comprises a 1.alpha.-fold
motif of a .beta.-trefoil domain of a Clostridial botulinum
serotype A HA-33, a 1.beta.-fold motif of a .beta.-trefoil domain
of a Clostridial botulinum serotype A HA-33, a 1.gamma.-fold motif
of a .beta.-trefoil domain of a Clostridial botulinum serotype A
HA-33, a 2.alpha.-fold motif of a .beta.-trefoil domain of a
Clostridial botulinum serotype A HA-33, a 2.beta.-fold motif of a
.beta.-trefoil domain of a Clostridial botulinum serotype A HA-33,
or a 2.gamma.-fold motif of a .beta.-trefoil domain of a
Clostridial botulinum serotype A HA-33 of SEQ ID NO: 13. In another
aspect of this embodiment, a .beta.-trefoil domain derived from a
Clostridial botulinum serotype A HA-33 comprises amino acids 10-54,
amino acids 60-100, amino acids 105-144, amino acids 151-195, amino
acids 200-242, or amino acids 249-293 of SEQ ID NO: 13.
[0395] In another embodiment, a .beta.-trefoil domain with enhanced
binding activity derived from a Clostridial botulinum serotype A
HA-33 comprises a .beta.-trefoil domain with enhanced binding
activity derived from a Clostridial botulinum serotype A HA-33 of
SEQ ID NO: 13. In another embodiment, a .beta.-trefoil domain with
enhanced binding activity derived from a Clostridial botulinum
serotype A HA-33 comprises amino acids 10-144 or amino acids
151-293 of SEQ ID NO: 13. In another aspect of this embodiment, a
.beta.-trefoil domain with enhanced binding activity derived from a
Clostridial botulinum serotype A HA-33 comprises a modified
1.alpha.-fold motif of a .beta.-trefoil domain of a Clostridial
botulinum serotype A HA-33, a modified 1.beta.-fold motif of a
.beta.-trefoil domain of a Clostridial botulinum serotype A HA-33,
a modified 1.gamma.-fold motif of a .beta.-trefoil domain of a
Clostridial botulinum serotype A HA-33, a modified 2.alpha.-fold
motif of a .beta.-trefoil domain of a Clostridial botulinum
serotype A HA-33, a modified 2.beta.-fold motif of a .beta.-trefoil
domain of a Clostridial botulinum serotype A HA-33, or a modified
2.gamma.-fold motif of a .beta.-trefoil domain of a Clostridial
botulinum serotype A HA-33 of SEQ ID NO: 13. In another aspect of
this embodiment, a .beta.-trefoil domain with enhanced binding
activity derived from a Clostridial botulinum serotype A HA-33
comprises amino acids 10-54, amino acids 60-100, amino acids
105-144, amino acids 151-195, amino acids 200-242, or amino acids
249-293 of SEQ ID NO: 13.
[0396] In other aspects of this embodiment, a Clostridial botulinum
serotype A HA-33 comprising a .beta.-trefoil domain with enhanced
binding activity comprises a polypeptide having, e.g., at least 70%
amino acid identity with amino acids 10-55, amino acids 56-102,
amino acids 103-144, amino acids 151-197, amino acids 198-245, or
amino acids 246-279 of SEQ ID NO: 13, at least 75% amino acid
identity with amino acids 10-55, amino acids 56-102, amino acids
103-144, amino acids 151-197, amino acids 198-245, or amino acids
246-279 of SEQ ID NO: 13, at least 80% amino acid identity with
amino acids 10-55, amino acids 56-102, amino acids 103-144, amino
acids 151-197, amino acids 198-245, or amino acids 246-279 of SEQ
ID NO: 13, at least 85% amino acid identity with amino acids 10-55,
amino acids 56-102, amino acids 103-144, amino acids 151-197, amino
acids 198-245, or amino acids 246-279 of SEQ ID NO: 13, at least
90% amino acid identity with amino acids 10-55, amino acids 56-102,
amino acids 103-144, amino acids 151-197, amino acids 198-245, or
amino acids 246-279 of SEQ ID NO: 13 or at least 95% amino acid
identity with amino acids 10-55, amino acids 56-102, amino acids
103-144, amino acids 151-197, amino acids 198-245, or amino acids
246-279 of SEQ ID NO: 13. In yet other aspects of this embodiment,
a Clostridial botulinum serotype A HA-33 comprising a
.beta.-trefoil domain with enhanced binding activity comprises a
polypeptide having, e.g., at most 70% amino acid identity with
amino acids 10-55, amino acids 56-102, amino acids 103-144, amino
acids 151-197, amino acids 198-245, or amino acids 246-279 of SEQ
ID NO: 13, at most 75% amino acid identity with amino acids 10-55,
amino acids 56-102, amino acids 103-144, amino acids 151-197, amino
acids 198-245, or amino acids 246-279 of SEQ ID NO: 13, at most 80%
amino acid identity with amino acids 10-55, amino acids 56-102,
amino acids 103-144, amino acids 151-197, amino acids 198-245, or
amino acids 246-279 of SEQ ID NO: 13, at most 85% amino acid
identity with amino acids 10-55, amino acids 56-102, amino acids
103-144, amino acids 151-197, amino acids 198-245, or amino acids
246-279 of SEQ ID NO: 13, at most 90% amino acid identity with
amino acids 10-55, amino acids 56-102, amino acids 103-144, amino
acids 151-197, amino acids 198-245, or amino acids 246-279 of SEQ
ID NO: 13 or at most 95% amino acid identity with amino acids
10-55, amino acids 56-102, amino acids 103-144, amino acids
151-197, amino acids 198-245, or amino acids 246-279 of SEQ ID NO:
13.
[0397] In other aspects of this embodiment, a Clostridial botulinum
serotype A HA-33 comprising a .beta.-trefoil domain with enhanced
binding activity comprises a polypeptide having, e.g., at most one,
two, three, four, five, six, seven, eight, nine, 10 or 20
non-contiguous amino acid substitutions relative to amino acids
10-55, amino acids 56-102, amino acids 103-144, amino acids
151-197, amino acids 198-245, or amino acids 246-279 of SEQ ID NO:
13. In other aspects of this embodiment, a Clostridial botulinum
serotype A HA-33 comprising a .beta.-trefoil domain with enhanced
binding activity comprises a polypeptide having, e.g., at least
one, two, three, four, five, six, seven, eight, nine, 10 or 20
non-contiguous amino acid substitutions relative to amino acids
10-55, amino acids 56-102, amino acids 103-144, amino acids
151-197, amino acids 198-245, or amino acids 246-279 of SEQ ID NO:
13. In yet other aspects of this embodiment, a Clostridial
botulinum serotype A HA-33 comprising a .beta.-trefoil domain with
enhanced binding activity comprises a polypeptide having, e.g., at
most one, two, three, four, five, six, seven, eight, nine, 10 or 20
non-contiguous amino acid deletions relative to amino acids 10-55,
amino acids 56-102, amino acids 103-144, amino acids 151-197, amino
acids 198-245, or amino acids 246-279 of SEQ ID NO: 13. In other
aspects of this embodiment, a Clostridial botulinum serotype A
HA-33 comprising a .beta.-trefoil domain with enhanced binding
activity comprises a polypeptide having, e.g., at least one, two,
three, four, five, six, seven, eight, nine, 10 or 20 non-contiguous
amino acid deletions relative to amino acids 10-55, amino acids
56-102, amino acids 103-144, amino acids 151-197, amino acids
198-245, or amino acids 246-279 of SEQ ID NO: 13. In still other
aspects of this embodiment, a Clostridial botulinum serotype A
HA-33 comprising a .beta.-trefoil domain with enhanced binding
activity comprises a polypeptide having, e.g., at most one, two,
three, four, five, six, seven, eight, nine, 10 or 20 non-contiguous
amino acid additions relative to amino acids 10-55, amino acids
56-102, amino acids 103-144, amino acids 151-197, amino acids
198-245, or amino acids 246-279 of SEQ ID NO: 13. In other aspects
of this embodiment, a Clostridial botulinum serotype A HA-33
comprising a .beta.-trefoil domain with enhanced binding activity
comprises a polypeptide having, e.g., at least one, two, three,
four, five, six, seven, eight, nine, 10 or 20 non-contiguous amino
acid additions relative to amino acids 10-55, amino acids 56-102,
amino acids 103-144, amino acids 151-197, amino acids 198-245, or
amino acids 246-279 of SEQ ID NO: 13.
[0398] In other aspects of this embodiment, a Clostridial botulinum
serotype A HA-33 comprising a .beta.-trefoil domain with enhanced
binding activity comprises a polypeptide having, e.g., at most one,
two, three, four, five, six, seven, eight, nine, 10 or 20
contiguous amino acid substitutions relative to amino acids 10-55,
amino acids 56-102, amino acids 103-144, amino acids 151-197, amino
acids 198-245, or amino acids 246-279 of SEQ ID NO: 13. In other
aspects of this embodiment, a Clostridial botulinum serotype A
HA-33 comprising a .beta.-trefoil domain with enhanced binding
activity comprises a polypeptide having, e.g., at least one, two,
three, four, five, six, seven, eight, nine, 10 or 20 contiguous
amino acid substitutions relative to amino acids 10-55, amino acids
56-102, amino acids 103-144, amino acids 151-197, amino acids
198-245, or amino acids 246-279 of SEQ ID NO: 13. In yet other
aspects of this embodiment, a Clostridial botulinum serotype A
HA-33 comprising a .beta.-trefoil domain with enhanced binding
activity comprises a polypeptide having, e.g., at most one, two,
three, four, five, six, seven, eight, nine, 10 or 20 contiguous
amino acid deletions relative to amino acids 10-55, amino acids
56-102, amino acids 103-144, amino acids 151-197, amino acids
198-245, or amino acids 246-279 of SEQ ID NO: 13. In other aspects
of this embodiment, a Clostridial botulinum serotype A HA-33
comprising a .beta.-trefoil domain with enhanced binding activity
comprises a polypeptide having, e.g., at least one, two, three,
four, five, six, seven, eight, nine, 10 or 20 contiguous amino acid
deletions relative to amino acids 10-55, amino acids 56-102, amino
acids 103-144, amino acids 151-197, amino acids 198-245, or amino
acids 246-279 of SEQ ID NO: 13. In still other aspects of this
embodiment, a Clostridial botulinum serotype A HA-33 comprising a
.beta.-trefoil domain with enhanced binding activity comprises a
polypeptide having, e.g., at most one, two, three, four, five, six,
seven, eight, nine, 10 or 20 contiguous amino acid additions
relative to amino acids 10-55, amino acids 56-102, amino acids
103-144, amino acids 151-197, amino acids 198-245, or amino acids
246-279 of SEQ ID NO: 13. In other aspects of this embodiment, a
Clostridial botulinum serotype A HA-33 comprising a .beta.-trefoil
domain with enhanced binding activity comprises a polypeptide
having, e.g., at least one, two, three, four, five, six, seven,
eight, nine, 10 or 20 contiguous amino acid additions relative to
amino acids 10-55, amino acids 56-102, amino acids 103-144, amino
acids 151-197, amino acids 198-245, or amino acids 246-279 of SEQ
ID NO: 13.
[0399] In another embodiment, a .beta.-trefoil domain derived from
a Clostridial botulinum serotype A HA-33 comprises a
1.beta.4/.beta.5 hairpin turn of a .beta.-trefoil domain of a
Clostridial botulinum serotype A HA-33, a 1.beta.8/.beta.9 hairpin
turn of a .beta.-trefoil domain of a Clostridial botulinum serotype
A HA-33, a 2.beta.4/.beta.5 hairpin turn of a .beta.-trefoil domain
of a Clostridial botulinum serotype A HA-33 or a 2.beta.8/.beta.9
hairpin turn of a .beta.-trefoil domain of a Clostridial botulinum
serotype A HA-33 of SEQ ID NO: 13. In another aspect of this
embodiment, a .beta.-trefoil domain derived from a Clostridial
botulinum serotype A HA-33 comprises amino acids 55-59, amino acids
101-104, amino acids 196-199, or amino acids 243-248 of SEQ ID NO:
13.
[0400] In another embodiment, a .beta.-trefoil domain with enhanced
binding activity derived from a Clostridial botulinum serotype A
HA-33 comprises a modified 1.beta.4/.beta.5 hairpin turn of a
.beta.-trefoil domain of a Clostridial botulinum serotype A HA-33,
a modified 1.beta.8/.beta.9 hairpin turn of a .beta.-trefoil domain
of a Clostridial botulinum serotype A HA-33, a modified
2.beta.4/.beta.5 hairpin turn of a .beta.-trefoil domain of a
Clostridial botulinum serotype A HA-33 or a modified
2.beta.8/.beta.9 hairpin turn of a .beta.-trefoil domain of a
Clostridial botulinum serotype A HA-33 of SEQ ID NO: 13. In another
aspect of this embodiment, a .beta.-trefoil domain with enhanced
binding activity derived from a Clostridial botulinum serotype A
HA-33 comprises a modification of amino acids 55-59, amino acids
101-104, amino acids 196-199, or amino acids 243-248 of SEQ ID NO:
13.
[0401] In other aspects of this embodiment, a .beta.-trefoil domain
with enhanced binding activity derived from a Clostridial botulinum
serotype A HA-33 comprises a polypeptide having, e.g., at least 70%
amino acid identity with amino acids 55-59, amino acids 101-104,
amino acids 196-199, or amino acids 243-248 of SEQ ID NO: 13, at
least 75% amino acid identity with amino acids 55-59, amino acids
101-104, amino acids 196-199, or amino acids 243-248 of SEQ ID NO:
13, at least 80% amino acid identity with amino acids 55-59, amino
acids 101-104, amino acids 196-199, or amino acids 243-248 of SEQ
ID NO: 13, at least 85% amino acid identity with amino acids 55-59,
amino acids 101-104, amino acids 196-199, or amino acids 243-248 of
SEQ ID NO: 13, at least 90% amino acid identity with amino acids
55-59, amino acids 101-104, amino acids 196-199, or amino acids
243-248 of SEQ ID NO: 13 or at least 95% amino acid identity with
amino acids 55-59, amino acids 101-104, amino acids 196-199, or
amino acids 243-248 of SEQ ID NO: 13. In yet other aspects of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a Clostridial botulinum serotype A HA-33 comprises a
polypeptide having, e.g., at most 70% amino acid identity with
amino acids 55-59, amino acids 101-104, amino acids 196-199, or
amino acids 243-248 of SEQ ID NO: 13, at most 75% amino acid
identity with amino acids 55-59, amino acids 101-104, amino acids
196-199, or amino acids 243-248 of SEQ ID NO: 13, at most 80% amino
acid identity with amino acids 55-59, amino acids 101-104, amino
acids 196-199, or amino acids 243-248 of SEQ ID NO: 13, at most 85%
amino acid identity with amino acids 55-59, amino acids 101-104,
amino acids 196-199, or amino acids 243-248 of SEQ ID NO: 13, at
most 90% amino acid identity with amino acids 55-59, amino acids
101-104, amino acids 196-199, or amino acids 243-248 of SEQ ID NO:
13 or at most 95% amino acid identity with amino acids 55-59, amino
acids 101-104, amino acids 196-199, or amino acids 243-248 of SEQ
ID NO: 13.
[0402] In other aspects of this embodiment, a .beta.-trefoil domain
with enhanced binding activity derived from a Clostridial botulinum
serotype A HA-33 comprises a polypeptide having, e.g., at most one,
two, three or four non-contiguous amino acid substitutions relative
to amino acids 55-59, amino acids 101-104, amino acids 196-199, or
amino acids 243-248 of SEQ ID NO: 13. In other aspects of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a Clostridial botulinum serotype A HA-33 comprises a
polypeptide having, e.g., at least one, two, three or four
non-contiguous amino acid substitutions relative to amino acids
55-59, amino acids 101-104, amino acids 196-199, or amino acids
243-248 of SEQ ID NO: 13. In other aspects of this embodiment, a
non-contiguous amino acid substitution of any amino acid from amino
acids 55-59, amino acids 101-104, amino acids 196-199, or amino
acids 243-248 of SEQ ID NO: 13 can be replaced with glycine. In
other aspects of this embodiment, a non-contiguous amino acid
substitution of any hydrophobic amino acid from amino acids 55-59,
amino acids 101-104, amino acids 196-199, or amino acids 243-248 of
SEQ ID NO: 13 can be replaced with phenylalanine. In yet other
aspects of this embodiment, a .beta.-trefoil domain with enhanced
binding activity derived from a Clostridial botulinum serotype A
HA-33 comprises a polypeptide having, e.g., at most one, two, three
or four non-contiguous amino acid deletions relative to amino acids
55-59, amino acids 101-104, amino acids 196-199, or amino acids
243-248 of SEQ ID NO: 13. In other aspects of this embodiment, a
.beta.-trefoil domain with enhanced binding activity derived from a
Clostridial botulinum serotype A HA-33 comprises a polypeptide
having, e.g., at least one, two, three or four non-contiguous amino
acid deletions relative to amino acids 55-59, amino acids 101-104,
amino acids 196-199, or amino acids 243-248 of SEQ ID NO: 13. In
still other aspects of this embodiment, a .beta.-trefoil domain
with enhanced binding activity derived from a Clostridial botulinum
serotype A HA-33 comprises a polypeptide having, e.g., at most one,
two, three or four non-contiguous amino acid additions relative to
amino acids 55-59, amino acids 101-104, amino acids 196-199, or
amino acids 243-248 of SEQ ID NO: 13. In other aspects of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a Clostridial botulinum serotype A HA-33 comprises a
polypeptide having, e.g., at least one, two, three or four
non-contiguous amino acid additions relative to amino acids 55-59,
amino acids 101-104, amino acids 196-199, or amino acids 243-248 of
SEQ ID NO: 13.
[0403] In other aspects of this embodiment, a .beta.-trefoil domain
with enhanced binding activity derived from a Clostridial botulinum
serotype A HA-33 comprises a polypeptide having, e.g., at most one,
two, three or four contiguous amino acid substitutions relative to
amino acids 55-59, amino acids 101-104, amino acids 196-199, or
amino acids 243-248 of SEQ ID NO: 13. In other aspects of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a Clostridial botulinum serotype A HA-33 comprises a
polypeptide having, e.g., at least one, two, three or four
contiguous amino acid substitutions relative to amino acids 55-59,
amino acids 101-104, amino acids 196-199, or amino acids 243-248 of
SEQ ID NO: 13. In other aspects of this embodiment, contiguous
amino acid substitutions of amino acids from amino acids 55-59,
amino acids 101-104, amino acids 196-199, or amino acids 243-248 of
SEQ ID NO: 13 can be replaced with glycine. In other aspects of
this embodiment, contiguous amino acid substitutions of hydrophobic
amino acids from amino acids 55-59, amino acids 101-104, amino
acids 196-199, or amino acids 243-248 of SEQ ID NO: 13 can be
replaced with phenylalanine. In yet other aspects of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a Clostridial botulinum serotype A HA-33 comprises a
polypeptide having, e.g., at most one, two, three or four
contiguous amino acid deletions relative to amino acids 55-59,
amino acids 101-104, amino acids 196-199, or amino acids 243-248 of
SEQ ID NO: 13. In other aspects of this embodiment, a
.beta.-trefoil domain with enhanced binding activity derived from a
Clostridial botulinum serotype A HA-33 comprises a polypeptide
having, e.g., at least one, two, three or four contiguous amino
acid deletions relative to amino acids 55-59, amino acids 101-104,
amino acids 196-199, or amino acids 243-248 of SEQ ID NO: 13. In
still other aspects of this embodiment, a .beta.-trefoil domain
with enhanced binding activity derived from a Clostridial botulinum
serotype A HA-33 comprises a polypeptide having, e.g., at most one,
two, three or four contiguous amino acid additions relative to
amino acids 55-59, amino acids 101-104, amino acids 196-199, or
amino acids 243-248 of SEQ ID NO: 13. In other aspects of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a Clostridial botulinum serotype A HA-33 comprises a
polypeptide having, e.g., at least one, two, three or four
contiguous amino acid additions relative to amino acids 55-59,
amino acids 101-104, amino acids 196-199, or amino acids 243-248 of
SEQ ID NO: 13.
[0404] In another embodiment, a .beta.-trefoil domain derived from
a Clostridial botulinum serotype B HA-33 comprises a .beta.-trefoil
domain derived from a Clostridial botulinum serotype B HA-33 of SEQ
ID NO: 14. In another embodiment, a .beta.-trefoil domain derived
from a Clostridial botulinum serotype B HA-33 comprises amino acids
10-144 or amino acids 151-292 of SEQ ID NO: 14. In another aspect
of this embodiment, a .beta.-trefoil domain derived from a
Clostridial botulinum serotype B HA-33 comprises a l.alpha.-fold
motif of a 13-trefoil domain of a Clostridial botulinum serotype B
HA-33, a 1.beta.-fold motif of a .beta.-trefoil domain of a
Clostridial botulinum serotype B HA-33, a 1.gamma.-fold motif of a
.beta.-trefoil domain of a Clostridial botulinum serotype B HA-33,
a 2.alpha.-fold motif of a .beta.-trefoil domain of a Clostridial
botulinum serotype B HA-33, a 2.beta.-fold motif of a
.beta.-trefoil domain of a Clostridial botulinum serotype B HA-33,
or a 2.gamma.-fold motif of a .beta.-trefoil domain of a
Clostridial botulinum serotype B HA-33 of SEQ ID NO: 14. In another
aspect of this embodiment, a .beta.-trefoil domain derived from a
Clostridial botulinum serotype B HA-33 comprises amino acids 10-54,
amino acids 60-100, amino acids 105-144, amino acids 151-195, amino
acids 200-241, or amino acids 248-292 of SEQ ID NO: 14.
[0405] In another embodiment, a .beta.-trefoil domain with enhanced
binding activity derived from a Clostridial botulinum serotype B
HA-33 comprises a .beta.-trefoil domain with enhanced binding
activity derived from a Clostridial botulinum serotype B HA-33 of
SEQ ID NO: 14. In another embodiment, a .beta.-trefoil domain with
enhanced binding activity derived from a Clostridial botulinum
serotype B HA-33 comprises amino acids 10-144 or amino acids
151-292 of SEQ ID NO: 14. In another aspect of this embodiment, a
.beta.-trefoil domain with enhanced binding activity derived from a
Clostridial botulinum serotype B HA-33 comprises a modified
1.alpha.-fold motif of a .beta.-trefoil domain of a Clostridial
botulinum serotype B HA-33, a modified 1.beta.-fold motif of a
.beta.-trefoil domain of a Clostridial botulinum serotype B HA-33,
a modified 1.gamma.-fold motif of a .beta.-trefoil domain of a
Clostridial botulinum serotype B HA-33, a modified 2.alpha.-fold
motif of a .beta.-trefoil domain of a Clostridial botulinum
serotype B HA-33, a modified 2.beta.-fold motif of a .beta.-trefoil
domain of a Clostridial botulinum serotype B HA-33, or a modified
2.gamma.-fold motif of a .beta.-trefoil domain of a Clostridial
botulinum serotype B HA-33 of SEQ ID NO: 14. In another aspect of
this embodiment, a .beta.-trefoil domain with enhanced binding
activity derived from a Clostridial botulinum serotype B HA-33
comprises amino acids 10-54, amino acids 60-100, amino acids
105-144, amino acids 151-195, amino acids 200-241, or amino acids
248-292 of SEQ ID NO: 14.
[0406] In other aspects of this embodiment, a Clostridial botulinum
serotype B HA-33 comprising a .beta.-trefoil domain with enhanced
binding activity comprises a polypeptide having, e.g., at least 70%
amino acid identity with amino acids 10-54, amino acids 60-100,
amino acids 105-144, amino acids 151-195, amino acids 200-241, or
amino acids 248-292 of SEQ ID NO: 14, at least 75% amino acid
identity with amino acids 10-54, amino acids 60-100, amino acids
105-144, amino acids 151-195, amino acids 200-241, or amino acids
248-292 of SEQ ID NO: 14, at least 80% amino acid identity with
amino acids 10-54, amino acids 60-100, amino acids 105-144, amino
acids 151-195, amino acids 200-241, or amino acids 248-292 of SEQ
ID NO: 14, at least 85% amino acid identity with amino acids 10-54,
amino acids 60-100, amino acids 105-144, amino acids 151-195, amino
acids 200-241, or amino acids 248-292 of SEQ ID NO: 14, at least
90% amino acid identity with amino acids 10-54, amino acids 60-100,
amino acids 105-144, amino acids 151-195, amino acids 200-241, or
amino acids 248-292 of SEQ ID NO: 14 or at least 95% amino acid
identity with amino acids 10-54, amino acids 60-100, amino acids
105-144, amino acids 151-195, amino acids 200-241, or amino acids
248-292 of SEQ ID NO: 14. In yet other aspects of this embodiment,
a Clostridial botulinum serotype B HA-33 comprising a
.beta.-trefoil domain with enhanced binding activity comprises a
polypeptide having, e.g., at most 70% amino acid identity with
amino acids 10-54, amino acids 60-100, amino acids 105-144, amino
acids 151-195, amino acids 200-241, or amino acids 248-292 of SEQ
ID NO: 14, at most 75% amino acid identity with amino acids 10-54,
amino acids 60-100, amino acids 105-144, amino acids 151-195, amino
acids 200-241, or amino acids 248-292 of SEQ ID NO: 14, at most 80%
amino acid identity with amino acids 10-54, amino acids 60-100,
amino acids 105-144, amino acids 151-195, amino acids 200-241, or
amino acids 248-292 of SEQ ID NO: 14, at most 85% amino acid
identity with amino acids 10-54, amino acids 60-100, amino acids
105-144, amino acids 151-195, amino acids 200-241, or amino acids
248-292 of SEQ ID NO: 14, at most 90% amino acid identity with
amino acids 10-54, amino acids 60-100, amino acids 105-144, amino
acids 151-195, amino acids 200-241, or amino acids 248-292 of SEQ
ID NO: 14 or at most 95% amino acid identity with amino acids
10-54, amino acids 60-100, amino acids 105-144, amino acids
151-195, amino acids 200-241, or amino acids 248-292 of SEQ ID NO:
14.
[0407] In other aspects of this embodiment, a Clostridial botulinum
serotype B HA-33 comprising a .beta.-trefoil domain with enhanced
binding activity comprises a polypeptide having, e.g., at most one,
two, three, four, five, six, seven, eight, nine, 10 or 20
non-contiguous amino acid substitutions relative to amino acids
10-54, amino acids 60-100, amino acids 105-144, amino acids
151-195, amino acids 200-241, or amino acids 248-292 of SEQ ID NO:
14. In other aspects of this embodiment, a Clostridial botulinum
serotype B HA-33 comprising a .beta.-trefoil domain with enhanced
binding activity comprises a polypeptide having, e.g., at least
one, two, three, four, five, six, seven, eight, nine, 10 or 20
non-contiguous amino acid substitutions relative to amino acids
10-54, amino acids 60-100, amino acids 105-144, amino acids
151-195, amino acids 200-241, or amino acids 248-292 of SEQ ID NO:
14. In yet other aspects of this embodiment, a Clostridial
botulinum serotype B HA-33 comprising a .beta.-trefoil domain with
enhanced binding activity comprises a polypeptide having, e.g., at
most one, two, three, four, five, six, seven, eight, nine, 10 or 20
non-contiguous amino acid deletions relative to amino acids 10-54,
amino acids 60-100, amino acids 105-144, amino acids 151-195, amino
acids 200-241, or amino acids 248-292 of SEQ ID NO: 14. In other
aspects of this embodiment, a Clostridial botulinum serotype B
HA-33 comprising a .beta.-trefoil domain with enhanced binding
activity comprises a polypeptide having, e.g., at least one, two,
three, four, five, six, seven, eight, nine, 10 or 20 non-contiguous
amino acid deletions relative to amino acids 10-54, amino acids
60-100, amino acids 105-144, amino acids 151-195, amino acids
200-241, or amino acids 248-292 of SEQ ID NO: 14. In still other
aspects of this embodiment, a Clostridial botulinum serotype B
HA-33 comprising a .beta.-trefoil domain with enhanced binding
activity comprises a polypeptide having, e.g., at most one, two,
three, four, five, six, seven, eight, nine, 10 or 20 non-contiguous
amino acid additions relative to amino acids 10-54, amino acids
60-100, amino acids 105-144, amino acids 151-195, amino acids
200-241, or amino acids 248-292 of SEQ ID NO: 14. In other aspects
of this embodiment, a Clostridial botulinum serotype B HA-33
comprising a .beta.-trefoil domain with enhanced binding activity
comprises a polypeptide having, e.g., at least one, two, three,
four, five, six, seven, eight, nine, 10 or 20 non-contiguous amino
acid additions relative to amino acids 10-54, amino acids 60-100,
amino acids 105-144, amino acids 151-195, amino acids 200-241, or
amino acids 248-292 of SEQ ID NO: 14.
[0408] In other aspects of this embodiment, a Clostridial botulinum
serotype B HA-33 comprising a .beta.-trefoil domain with enhanced
binding activity comprises a polypeptide having, e.g., at most one,
two, three, four, five, six, seven, eight, nine, 10 or 20
contiguous amino acid substitutions relative to amino acids 10-54,
amino acids 60-100, amino acids 105-144, amino acids 151-195, amino
acids 200-241, or amino acids 248-292 of SEQ ID NO: 14. In other
aspects of this embodiment, a Clostridial botulinum serotype B
HA-33 comprising a .beta.-trefoil domain with enhanced binding
activity comprises a polypeptide having, e.g., at least one, two,
three, four, five, six, seven, eight, nine, 10 or 20 contiguous
amino acid substitutions relative to amino acids 10-54, amino acids
60-100, amino acids 105-144, amino acids 151-195, amino acids
200-241, or amino acids 248-292 of SEQ ID NO: 14. In yet other
aspects of this embodiment, a Clostridial botulinum serotype B
HA-33 comprising a .beta.-trefoil domain with enhanced binding
activity comprises a polypeptide having, e.g., at most one, two,
three, four, five, six, seven, eight, nine, 10 or 20 contiguous
amino acid deletions relative to amino acids 10-54, amino acids
60-100, amino acids 105-144, amino acids 151-195, amino acids
200-241, or amino acids 248-292 of SEQ ID NO: 14. In other aspects
of this embodiment, a Clostridial botulinum serotype B HA-33
comprising a .beta.-trefoil domain with enhanced binding activity
comprises a polypeptide having, e.g., at least one, two, three,
four, five, six, seven, eight, nine, 10 or 20 contiguous amino acid
deletions relative to amino acids 10-54, amino acids 60-100, amino
acids 105-144, amino acids 151-195, amino acids 200-241, or amino
acids 248-292 of SEQ ID NO: 14. In still other aspects of this
embodiment, a Clostridial botulinum serotype B HA-33 comprising a
.beta.-trefoil domain with enhanced binding activity comprises a
polypeptide having, e.g., at most one, two, three, four, five, six,
seven, eight, nine, 10 or 20 contiguous amino acid additions
relative to amino acids 10-54, amino acids 60-100, amino acids
105-144, amino acids 151-195, amino acids 200-241, or amino acids
248-292 of SEQ ID NO: 14. In other aspects of this embodiment, a
Clostridial botulinum serotype B HA-33 comprising a .beta.-trefoil
domain with enhanced binding activity comprises a polypeptide
having, e.g., at least one, two, three, four, five, six, seven,
eight, nine, 10 or 20 contiguous amino acid additions relative to
amino acids 10-54, amino acids 60-100, amino acids 105-144, amino
acids 151-195, amino acids 200-241, or amino acids 248-292 of SEQ
ID NO: 14.
[0409] In another embodiment, a .beta.-trefoil domain derived from
a Clostridial botulinum serotype B HA-33 comprises a
1.beta.4/.beta.5 hairpin turn of a .beta.-trefoil domain of a
Clostridial botulinum serotype B HA-33, a 1.beta.8/.beta.9 hairpin
turn of a .beta.-trefoil domain of a Clostridial botulinum serotype
B HA-33, a 2.beta.4/.beta.5 hairpin turn of a .beta.-trefoil domain
of a Clostridial botulinum serotype B HA-33 or a 2.beta.8/.beta.9
hairpin turn of a .beta.-trefoil domain of a Clostridial botulinum
serotype B HA-33 of SEQ ID NO: 14. In another aspect of this
embodiment, a .beta.-trefoil domain derived from a Clostridial
botulinum serotype B HA-33 comprises amino acids 55-59, amino acids
101-104, amino acids 196-199, or amino acids 242-247 of SEQ ID NO:
14.
[0410] In another embodiment, a .beta.-trefoil domain with enhanced
binding activity derived from a Clostridial botulinum serotype B
HA-33 comprises a modified 1.beta.4/.beta.5 hairpin turn of a
.beta.-trefoil domain of a Clostridial botulinum serotype B HA-33,
a modified 1.beta.8/.beta.9 hairpin turn of a .beta.-trefoil domain
of a Clostridial botulinum serotype B HA-33, a modified
2.beta.4/.beta.5 hairpin turn of a .beta.-trefoil domain of a
Clostridial botulinum serotype B HA-33 or a modified
2.beta.8/.beta.9 hairpin turn of a .beta.-trefoil domain of a
Clostridial botulinum serotype B HA-33 of SEQ ID NO: 14. In another
aspect of this embodiment, a .beta.-trefoil domain with enhanced
binding activity derived from a Clostridial botulinum serotype B
HA-33 comprises a modification of amino acids 55-59, amino acids
101-104, amino acids 196-199, or amino acids 242-247 of SEQ ID NO:
14.
[0411] In other aspects of this embodiment, a .beta.-trefoil domain
with enhanced binding activity derived from a Clostridial botulinum
serotype B HA-33 comprises a polypeptide having, e.g., at least 70%
amino acid identity with amino acids 55-59, amino acids 101-104,
amino acids 196-199, or amino acids 242-247 of SEQ ID NO: 14, at
least 75% amino acid identity with amino acids 55-59, amino acids
101-104, amino acids 196-199, or amino acids 242-247 of SEQ ID NO:
14, at least 80% amino acid identity with amino acids 55-59, amino
acids 101-104, amino acids 196-199, or amino acids 242-247 of SEQ
ID NO: 14, at least 85% amino acid identity with amino acids 55-59,
amino acids 101-104, amino acids 196-199, or amino acids 242-247 of
SEQ ID NO: 14, at least 90% amino acid identity with amino acids
55-59, amino acids 101-104, amino acids 196-199, or amino acids
242-247 of SEQ ID NO: 14 or at least 95% amino acid identity with
amino acids 55-59, amino acids 101-104, amino acids 196-199, or
amino acids 242-247 of SEQ ID NO: 14. In yet other aspects of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a Clostridial botulinum serotype B HA-33 comprises a
polypeptide having, e.g., at most 70% amino acid identity with
amino acids 55-59, amino acids 101-104, amino acids 196-199, or
amino acids 242-247 of SEQ ID NO: 14, at most 75% amino acid
identity with amino acids 55-59, amino acids 101-104, amino acids
196-199, or amino acids 242-247 of SEQ ID NO: 14, at most 80% amino
acid identity with amino acids 55-59, amino acids 101-104, amino
acids 196-199, or amino acids 242-247 of SEQ ID NO: 14, at most 85%
amino acid identity with amino acids 55-59, amino acids 101-104,
amino acids 196-199, or amino acids 242-247 of SEQ ID NO: 14, at
most 90% amino acid identity with amino acids 55-59, amino acids
101-104, amino acids 196-199, or amino acids 242-247 of SEQ ID NO:
14 or at most 95% amino acid identity with amino acids 55-59, amino
acids 101-104, amino acids 196-199, or amino acids 242-247 of SEQ
ID NO: 14.
[0412] In other aspects of this embodiment, a .beta.-trefoil domain
with enhanced binding activity derived from a Clostridial botulinum
serotype B HA-33 comprises a polypeptide having, e.g., at most one,
two, three or four non-contiguous amino acid substitutions relative
to amino acids 55-59, amino acids 101-104, amino acids 196-199, or
amino acids 242-247 of SEQ ID NO: 14. In other aspects of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a Clostridial botulinum serotype B HA-33 comprises a
polypeptide having, e.g., at least one, two, three or four
non-contiguous amino acid substitutions relative to amino acids
55-59, amino acids 101-104, amino acids 196-199, or amino acids
242-247 of SEQ ID NO: 14. In other aspects of this embodiment, a
non-contiguous amino acid substitution of any amino acid from amino
acids 55-59, amino acids 101-104, amino acids 196-199, or amino
acids 242-247 of SEQ ID NO: 14 can be replaced with glycine. In
other aspects of this embodiment, a non-contiguous amino acid
substitution of any hydrophobic amino acid from amino acids 55-59,
amino acids 101-104, amino acids 196-199, or amino acids 242-247 of
SEQ ID NO: 14 can be replaced with phenylalanine. In yet other
aspects of this embodiment, a .beta.-trefoil domain with enhanced
binding activity derived from a Clostridial botulinum serotype B
HA-33 comprises a polypeptide having, e.g., at most one, two, three
or four non-contiguous amino acid deletions relative to amino acids
55-59, amino acids 101-104, amino acids 196-199, or amino acids
242-247 of SEQ ID NO: 14. In other aspects of this embodiment, a
.beta.-trefoil domain with enhanced binding activity derived from a
Clostridial botulinum serotype B HA-33 comprises a polypeptide
having, e.g., at least one, two, three or four non-contiguous amino
acid deletions relative to amino acids 55-59, amino acids 101-104,
amino acids 196-199, or amino acids 242-247 of SEQ ID NO: 14. In
still other aspects of this embodiment, a .beta.-trefoil domain
with enhanced binding activity derived from a Clostridial botulinum
serotype B HA-33 comprises a polypeptide having, e.g., at most one,
two, three or four non-contiguous amino acid additions relative to
amino acids 55-59, amino acids 101-104, amino acids 196-199, or
amino acids 242-247 of SEQ ID NO: 14. In other aspects of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a Clostridial botulinum serotype B HA-33 comprises a
polypeptide having, e.g., at least one, two, three or four
non-contiguous amino acid additions relative to amino acids 55-59,
amino acids 101-104, amino acids 196-199, or amino acids 242-247 of
SEQ ID NO: 14.
[0413] In other aspects of this embodiment, a .beta.-trefoil domain
with enhanced binding activity derived from a Clostridial botulinum
serotype B HA-33 comprises a polypeptide having, e.g., at most one,
two, three or four contiguous amino acid substitutions relative to
amino acids 55-59, amino acids 101-104, amino acids 196-199, or
amino acids 242-247 of SEQ ID NO: 14. In other aspects of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a Clostridial botulinum serotype B HA-33 comprises a
polypeptide having, e.g., at least one, two, three or four
contiguous amino acid substitutions relative to amino acids 55-59,
amino acids 101-104, amino acids 196-199, or amino acids 242-247 of
SEQ ID NO: 14. In other aspects of this embodiment, contiguous
amino acid substitutions of amino acids from amino acids 55-59,
amino acids 101-104, amino acids 196-199, or amino acids 242-247 of
SEQ ID NO: 14 can be replaced with glycine. In other aspects of
this embodiment, contiguous amino acid substitutions of hydrophobic
amino acids from amino acids 55-59, amino acids 101-104, amino
acids 196-199, or amino acids 242-247 of SEQ ID NO: 14 can be
replaced with phenylalanine. In yet other aspects of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a Clostridial botulinum serotype B HA-33 comprises a
polypeptide having, e.g., at most one, two, three or four
contiguous amino acid deletions relative to amino acids 55-59,
amino acids 101-104, amino acids 196-199, or amino acids 242-247 of
SEQ ID NO: 14. In other aspects of this embodiment, a
.beta.-trefoil domain with enhanced binding activity derived from a
Clostridial botulinum serotype B HA-33 comprises a polypeptide
having, e.g., at least one, two, three or four contiguous amino
acid deletions relative to amino acids 55-59, amino acids 101-104,
amino acids 196-199, or amino acids 242-247 of SEQ ID NO: 14. In
still other aspects of this embodiment, a .beta.-trefoil domain
with enhanced binding activity derived from a Clostridial botulinum
serotype B HA-33 comprises a polypeptide having, e.g., at most one,
two, three or four contiguous amino acid additions relative to
amino acids 55-59, amino acids 101-104, amino acids 196-199, or
amino acids 242-247 of SEQ ID NO: 14. In other aspects of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a Clostridial botulinum serotype B HA-33 comprises a
polypeptide having, e.g., at least one, two, three or four
contiguous amino acid additions relative to amino acids 55-59,
amino acids 101-104, amino acids 196-199, or amino acids 242-247 of
SEQ ID NO: 14.
[0414] In another embodiment, a .beta.-trefoil domain with enhanced
binding activity derived from a Clostridial botulinum serotype B
HA-33 comprises a .beta.-trefoil domain with enhanced binding
activity derived from a Clostridial botulinum serotype B HA-33 of
SEQ ID NO: 15. In another aspect of this embodiment, a
.beta.-trefoil domain with enhanced binding activity derived from a
Clostridial botulinum serotype B HA-33 comprises amino acids 10-56,
amino acids 62-102, amino acids 107-146, amino acids 153-197, amino
acids 200-242, or amino acids 249-291 of SEQ ID NO: 15.
[0415] In other aspects of this embodiment, a Clostridial botulinum
serotype B HA-33 comprising a .beta.-trefoil domain with enhanced
binding activity comprises a polypeptide having, e.g., at least 70%
amino acid identity with amino acids 10-56, amino acids 62-102,
amino acids 107-146, amino acids 153-197, amino acids 200-242, or
amino acids 249-291 of SEQ ID NO: 15, at least 75% amino acid
identity with amino acids 10-56, amino acids 62-102, amino acids
107-146, amino acids 153-197, amino acids 200-242, or amino acids
249-291 of SEQ ID NO: 15, at least 80% amino acid identity with
amino acids 10-56, amino acids 62-102, amino acids 107-146, amino
acids 153-197, amino acids 200-242, or amino acids 249-291 of SEQ
ID NO: 15, at least 85% amino acid identity with amino acids 10-56,
amino acids 62-102, amino acids 107-146, amino acids 153-197, amino
acids 200-242, or amino acids 249-291 of SEQ ID NO: 15, at least
90% amino acid identity with amino acids 10-56, amino acids 62-102,
amino acids 107-146, amino acids 153-197, amino acids 200-242, or
amino acids 249-291 of SEQ ID NO: 15 or at least 95% amino acid
identity with amino acids 10-56, amino acids 62-102, amino acids
107-146, amino acids 153-197, amino acids 200-242, or amino acids
249-291 of SEQ ID NO: 15. In yet other aspects of this embodiment,
a Clostridial botulinum serotype B HA-33 comprising a
.beta.-trefoil domain with enhanced binding activity comprises a
polypeptide having, e.g., at most 70% amino acid identity with
amino acids 10-56, amino acids 62-102, amino acids 107-146, amino
acids 153-197, amino acids 200-242, or amino acids 249-291 of SEQ
ID NO: 15, at most 75% amino acid identity with amino acids 10-56,
amino acids 62-102, amino acids 107-146, amino acids 153-197, amino
acids 200-242, or amino acids 249-291 of SEQ ID NO: 15, at most 80%
amino acid identity with amino acids 10-56, amino acids 62-102,
amino acids 107-146, amino acids 153-197, amino acids 200-242, or
amino acids 249-291 of SEQ ID NO: 15, at most 85% amino acid
identity with amino acids 10-56, amino acids 62-102, amino acids
107-146, amino acids 153-197, amino acids 200-242, or amino acids
249-291 of SEQ ID NO: 15, at most 90% amino acid identity with
amino acids 10-56, amino acids 62-102, amino acids 107-146, amino
acids 153-197, amino acids 200-242, or amino acids 249-291 of SEQ
ID NO: 15 or at most 95% amino acid identity with amino acids
10-56, amino acids 62-102, amino acids 107-146, amino acids
153-197, amino acids 200-242, or amino acids 249-291 of SEQ ID NO:
15.
[0416] In other aspects of this embodiment, a Clostridial botulinum
serotype B HA-33 comprising a .beta.-trefoil domain with enhanced
binding activity comprises a polypeptide having, e.g., at most one,
two, three, four, five, six, seven, eight, nine, 10 or 20
non-contiguous amino acid substitutions relative to amino acids
10-56, amino acids 62-102, amino acids 107-146, amino acids
153-197, amino acids 200-242, or amino acids 249-291 of SEQ ID NO:
15. In other aspects of this embodiment, a Clostridial botulinum
serotype B HA-33 comprising a .beta.-trefoil domain with enhanced
binding activity comprises a polypeptide having, e.g., at least
one, two, three, four, five, six, seven, eight, nine, 10 or 20
non-contiguous amino acid substitutions relative to amino acids
10-56, amino acids 62-102, amino acids 107-146, amino acids
153-197, amino acids 200-242, or amino acids 249-291 of SEQ ID NO:
15. In yet other aspects of this embodiment, a Clostridial
botulinum serotype B HA-33 comprising a .beta.-trefoil domain with
enhanced binding activity comprises a polypeptide having, e.g., at
most one, two, three, four, five, six, seven, eight, nine, 10 or 20
non-contiguous amino acid deletions relative to amino acids 10-56,
amino acids 62-102, amino acids 107-146, amino acids 153-197, amino
acids 200-242, or amino acids 249-291 of SEQ ID NO: 15. In other
aspects of this embodiment, a Clostridial botulinum serotype B
HA-33 comprising a .beta.-trefoil domain with enhanced binding
activity comprises a polypeptide having, e.g., at least one, two,
three, four, five, six, seven, eight, nine, 10 or 20 non-contiguous
amino acid deletions relative to amino acids 10-56, amino acids
62-102, amino acids 107-146, amino acids 153-197, amino acids
200-242, or amino acids 249-291 of SEQ ID NO: 15. In still other
aspects of this embodiment, a Clostridial botulinum serotype B
HA-33 comprising a .beta.-trefoil domain with enhanced binding
activity comprises a polypeptide having, e.g., at most one, two,
three, four, five, six, seven, eight, nine, 10 or 20 non-contiguous
amino acid additions relative to amino acids 10-56, amino acids
62-102, amino acids 107-146, amino acids 153-197, amino acids
200-242, or amino acids 249-291 of SEQ ID NO: 15. In other aspects
of this embodiment, a Clostridial botulinum serotype B HA-33
comprising a .beta.-trefoil domain with enhanced binding activity
comprises a polypeptide having, e.g., at least one, two, three,
four, five, six, seven, eight, nine, 10 or 20 non-contiguous amino
acid additions relative to amino acids 10-56, amino acids 62-102,
amino acids 107-146, amino acids 153-197, amino acids 200-242, or
amino acids 249-291 of SEQ ID NO: 15.
[0417] In other aspects of this embodiment, a Clostridial botulinum
serotype B HA-33 comprising a .beta.-trefoil domain with enhanced
binding activity comprises a polypeptide having, e.g., at most one,
two, three, four, five, six, seven, eight, nine, 10 or 20
contiguous amino acid substitutions relative to amino acids 10-56,
amino acids 62-102, amino acids 107-146, amino acids 153-197, amino
acids 200-242, or amino acids 249-291 of SEQ ID NO: 15. In other
aspects of this embodiment, a Clostridial botulinum serotype B
HA-33 comprising a .beta.-trefoil domain with enhanced binding
activity comprises a polypeptide having, e.g., at least one, two,
three, four, five, six, seven, eight, nine, 10 or 20 contiguous
amino acid substitutions relative to amino acids 10-56, amino acids
62-102, amino acids 107-146, amino acids 153-197, amino acids
200-242, or amino acids 249-291 of SEQ ID NO: 15. In yet other
aspects of this embodiment, a Clostridial botulinum serotype B
HA-33 comprising a .beta.-trefoil domain with enhanced binding
activity comprises a polypeptide having, e.g., at most one, two,
three, four, five, six, seven, eight, nine, 10 or 20 contiguous
amino acid deletions relative to amino acids 10-56, amino acids
62-102, amino acids 107-146, amino acids 153-197, amino acids
200-242, or amino acids 249-291 of SEQ ID NO: 15. In other aspects
of this embodiment, a Clostridial botulinum serotype B HA-33
comprising a .beta.-trefoil domain with enhanced binding activity
comprises a polypeptide having, e.g., at least one, two, three,
four, five, six, seven, eight, nine, 10 or 20 contiguous amino acid
deletions relative to amino acids 10-56, amino acids 62-102, amino
acids 107-146, amino acids 153-197, amino acids 200-242, or amino
acids 249-291 of SEQ ID NO: 15. In still other aspects of this
embodiment, a Clostridial botulinum serotype B HA-33 comprising a
.beta.-trefoil domain with enhanced binding activity comprises a
polypeptide having, e.g., at most one, two, three, four, five, six,
seven, eight, nine, 10 or 20 contiguous amino acid additions
relative to amino acids 10-56, amino acids 62-102, amino acids
107-146, amino acids 153-197, amino acids 200-242, or amino acids
249-291 of SEQ ID NO: 15. In other aspects of this embodiment, a
Clostridial botulinum serotype B HA-33 comprising a .beta.-trefoil
domain with enhanced binding activity comprises a polypeptide
having, e.g., at least one, two, three, four, five, six, seven,
eight, nine, 10 or 20 contiguous amino acid additions relative to
amino acids 10-56, amino acids 62-102, amino acids 107-146, amino
acids 153-197, amino acids 200-242, or amino acids 249-291 of SEQ
ID NO: 15.
[0418] In another embodiment, a .beta.-trefoil domain derived from
a Clostridial botulinum serotype B HA-33 comprises a
1.beta.4/.beta.5 hairpin turn of a .beta.-trefoil domain of a
Clostridial botulinum serotype B HA-33, a 1.beta.8/.beta.9 hairpin
turn of a .beta.-trefoil domain of a Clostridial botulinum serotype
B HA-33, a 2.beta.4/.beta.5 hairpin turn of a .beta.-trefoil domain
of a Clostridial botulinum serotype B HA-33 or a 2.beta.88/.beta.9
hairpin turn of a .beta.-trefoil domain of a Clostridial botulinum
serotype B HA-33 of SEQ ID NO: 15. In another aspect of this
embodiment, a .beta.-trefoil domain derived from a Clostridial
botulinum serotype B HA-33 comprises amino acids 56-61, amino acids
103-106, amino acids 198-201, or amino acids 243-248 of SEQ ID NO:
15.
[0419] In another embodiment, a .beta.-trefoil domain with enhanced
binding activity derived from a Clostridial botulinum serotype B
HA-33 comprises a modified 1.beta.4/.beta.5 hairpin turn of a
.beta.-trefoil domain of a Clostridial botulinum serotype B HA-33,
a modified 1.beta.8/.beta.9 hairpin turn of a .beta.-trefoil domain
of a Clostridial botulinum serotype B HA-33, a modified
2.beta.4/.beta.5 hairpin turn of a .beta.-trefoil domain of a
Clostridial botulinum serotype B HA-33 or a modified
2.beta.88/.beta.9 hairpin turn of a .beta.-trefoil domain of a
Clostridial botulinum serotype B HA-33 of SEQ ID NO: 15. In another
aspect of this embodiment, a .beta.-trefoil domain with enhanced
binding activity derived from a Clostridial botulinum serotype B
HA-33 comprises a modification of amino acids 56-61, amino acids
103-106, amino acids 198-201, or amino acids 243-248 of SEQ ID NO:
15.
[0420] In other aspects of this embodiment, a .beta.-trefoil domain
with enhanced binding activity derived from a Clostridial botulinum
serotype B HA-33 comprises a polypeptide having, e.g., at least 70%
amino acid identity with amino acids 56-61, amino acids 103-106,
amino acids 198-201, or amino acids 243-248 of SEQ ID NO: 15, at
least 75% amino acid identity with amino acids 56-61, amino acids
103-106, amino acids 198-201, or amino acids 243-248 of SEQ ID NO:
15, at least 80% amino acid identity with amino acids 56-61, amino
acids 103-106, amino acids 198-201, or amino acids 243-248 of SEQ
ID NO: 15, at least 85% amino acid identity with amino acids 56-61,
amino acids 103-106, amino acids 198-201, or amino acids 243-248 of
SEQ ID NO: 15, at least 90% amino acid identity with amino acids
56-61, amino acids 103-106, amino acids 198-201, or amino acids
243-248 of SEQ ID NO: 15 or at least 95% amino acid identity with
amino acids 56-61, amino acids 103-106, amino acids 198-201, or
amino acids 243-248 of SEQ ID NO: 15. In yet other aspects of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a Clostridial botulinum serotype B HA-33 comprises a
polypeptide having, e.g., at most 70% amino acid identity with
amino acids 56-61, amino acids 103-106, amino acids 198-201, or
amino acids 243-248 of SEQ ID NO: 15, at most 75% amino acid
identity with amino acids 56-61, amino acids 103-106, amino acids
198-201, or amino acids 243-248 of SEQ ID NO: 15, at most 80% amino
acid identity with amino acids 56-61, amino acids 103-106, amino
acids 198-201, or amino acids 243-248 of SEQ ID NO: 15, at most 85%
amino acid identity with amino acids 56-61, amino acids 103-106,
amino acids 198-201, or amino acids 243-248 of SEQ ID NO: 15, at
most 90% amino acid identity with amino acids 56-61, amino acids
103-106, amino acids 198-201, or amino acids 243-248 of SEQ ID NO:
15 or at most 95% amino acid identity with amino acids 56-61, amino
acids 103-106, amino acids 198-201, or amino acids 243-248 of SEQ
ID NO: 15.
[0421] In other aspects of this embodiment, a .beta.-trefoil domain
with enhanced binding activity derived from a Clostridial botulinum
serotype B HA-33 comprises a polypeptide having, e.g., at most one,
two, three or four non-contiguous amino acid substitutions relative
to amino acids 56-61, amino acids 103-106, amino acids 198-201, or
amino acids 243-248 of SEQ ID NO: 15. In other aspects of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a Clostridial botulinum serotype B HA-33 comprises a
polypeptide having, e.g., at least one, two, three or four
non-contiguous amino acid substitutions relative to amino acids
56-61, amino acids 103-106, amino acids 198-201, or amino acids
243-248 of SEQ ID NO: 15. In other aspects of this embodiment, a
non-contiguous amino acid substitution of any amino acid from amino
acids 56-61, amino acids 103-106, amino acids 198-201, or amino
acids 243-248 of SEQ ID NO: 15 can be replaced with glycine. In
other aspects of this embodiment, a non-contiguous amino acid
substitution of any hydrophobic amino acid from amino acids 56-61,
amino acids 103-106, amino acids 198-201, or amino acids 243-248 of
SEQ ID NO: 15 can be replaced with phenylalanine. In yet other
aspects of this embodiment, a .beta.-trefoil domain with enhanced
binding activity derived from a Clostridial botulinum serotype B
HA-33 comprises a polypeptide having, e.g., at most one, two, three
or four non-contiguous amino acid deletions relative to amino acids
56-61, amino acids 103-106, amino acids 198-201, or amino acids
243-248 of SEQ ID NO: 15. In other aspects of this embodiment, a
.beta.-trefoil domain with enhanced binding activity derived from a
Clostridial botulinum serotype B HA-33 comprises a polypeptide
having, e.g., at least one, two, three or four non-contiguous amino
acid deletions relative to amino acids 56-61, amino acids 103-106,
amino acids 198-201, or amino acids 243-248 of SEQ ID NO: 15. In
still other aspects of this embodiment, a .beta.-trefoil domain
with enhanced binding activity derived from a Clostridial botulinum
serotype B HA-33 comprises a polypeptide having, e.g., at most one,
two, three or four non-contiguous amino acid additions relative to
amino acids 56-61, amino acids 103-106, amino acids 198-201, or
amino acids 243-248 of SEQ ID NO: 15. In other aspects of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a Clostridial botulinum serotype B HA-33 comprises a
polypeptide having, e.g., at least one, two, three or four
non-contiguous amino acid additions relative to amino acids 56-61,
amino acids 103-106, amino acids 198-201, or amino acids 243-248 of
SEQ ID NO: 15.
[0422] In other aspects of this embodiment, a .beta.-trefoil domain
with enhanced binding activity derived from a Clostridial botulinum
serotype B HA-33 comprises a polypeptide having, e.g., at most one,
two, three or four contiguous amino acid substitutions relative to
amino acids 56-61, amino acids 103-106, amino acids 198-201, or
amino acids 243-248 of SEQ ID NO: 15. In other aspects of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a Clostridial botulinum serotype B HA-33 comprises a
polypeptide having, e.g., at least one, two, three or four
contiguous amino acid substitutions relative to amino acids 56-61,
amino acids 103-106, amino acids 198-201, or amino acids 243-248 of
SEQ ID NO: 15. In other aspects of this embodiment, contiguous
amino acid substitutions of amino acids from amino acids 56-61,
amino acids 103-106, amino acids 198-201, or amino acids 243-248 of
SEQ ID NO: 15 can be replaced with glycine. In other aspects of
this embodiment, contiguous amino acid substitutions of hydrophobic
amino acids from amino acids 56-61, amino acids 103-106, amino
acids 198-201, or amino acids 243-248 of SEQ ID NO: 15 can be
replaced with phenylalanine. In yet other aspects of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a Clostridial botulinum serotype B HA-33 comprises a
polypeptide having, e.g., at most one, two, three or four
contiguous amino acid deletions relative to amino acids 56-61,
amino acids 103-106, amino acids 198-201, or amino acids 243-248 of
SEQ ID NO: 15. In other aspects of this embodiment, a
.beta.-trefoil domain with enhanced binding activity derived from a
Clostridial botulinum serotype B HA-33 comprises a polypeptide
having, e.g., at least one, two, three or four contiguous amino
acid deletions relative to amino acids 56-61, amino acids 103-106,
amino acids 198-201, or amino acids 243-248 of SEQ ID NO: 15. In
still other aspects of this embodiment, a .beta.-trefoil domain
with enhanced binding activity derived from a Clostridial botulinum
serotype B HA-33 comprises a polypeptide having, e.g., at most one,
two, three or four contiguous amino acid additions relative to
amino acids 56-61, amino acids 103-106, amino acids 198-201, or
amino acids 243-248 of SEQ ID NO: 15. In other aspects of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a Clostridial botulinum serotype B HA-33 comprises a
polypeptide having, e.g., at least one, two, three or four
contiguous amino acid additions relative to amino acids 56-61,
amino acids 103-106, amino acids 198-201, or amino acids 243-248 of
SEQ ID NO: 15.
[0423] In another embodiment, a .beta.-trefoil domain derived from
a Clostridial botulinum serotype C1 HA-33 comprises a
.beta.-trefoil domain derived from a Clostridial botulinum serotype
C1 HA-33 of SEQ ID NO: 16. In another embodiment, a .beta.-trefoil
domain derived from a Clostridial botulinum serotype C1 HA-33
comprises amino acids 10-141 or amino acids 148-285 of SEQ ID NO:
16. In another aspect of this embodiment, a .beta.-trefoil domain
derived from a Clostridial botulinum serotype C1 HA-33 comprises a
la-fold motif of a .beta.-trefoil domain of a Clostridial botulinum
serotype C1 HA-33, a 1.beta.-fold motif of a .beta.-trefoil domain
of a Clostridial botulinum serotype C1 HA-33, a 1.gamma.-fold motif
of a .beta.-trefoil domain of a Clostridial botulinum serotype C1
HA-33, a 2.alpha.-fold motif of a .beta.-trefoil domain of a
Clostridial botulinum serotype Cl HA-33, a 2.beta.-fold motif of a
.beta.-trefoil domain of a Clostridial botulinum serotype C1 HA-33,
or a 2.gamma.-fold motif of a .beta.-trefoil domain of a
Clostridial botulinum serotype C1 HA-33 of SEQ ID NO: 16. In
another aspect of this embodiment, a .beta.-trefoil domain derived
from a Clostridial botulinum serotype C1 HA-33 comprises amino
acids 10-54, amino acids 60-98, amino acids 103-141, amino acids
148-190, amino acids 195-234, or amino acids 241-285 of SEQ ID NO:
16.
[0424] In another embodiment, a .beta.-trefoil domain with enhanced
binding activity derived from a Clostridial botulinum serotype C1
HA-33 comprises a .beta.-trefoil domain with enhanced binding
activity derived from a Clostridial botulinum serotype C1 HA-33 of
SEQ ID NO: 16. In another embodiment, a .beta.-trefoil domain with
enhanced binding activity derived from a Clostridial botulinum
serotype C1 HA-33 comprises amino acids 10-141 or amino acids
148-285 of SEQ ID NO: 16. In another aspect of this embodiment, a
.beta.-trefoil domain with enhanced binding activity derived from a
Clostridial botulinum serotype C1 HA-33 comprises a modified
1.alpha.-fold motif of a .beta.-trefoil domain of a Clostridial
botulinum serotype C1 HA-33, a modified 1.beta.-fold motif of a
.beta.-trefoil domain of a Clostridial botulinum serotype C1 HA-33,
a modified 1.gamma.-fold motif of a .beta.-trefoil domain of a
Clostridial botulinum serotype C1 HA-33, a modified 2.alpha.-fold
motif of a .beta.-trefoil domain of a Clostridial botulinum
serotype C1 HA-33, a modified 2.beta.-fold motif of a
.beta.-trefoil domain of a Clostridial botulinum serotype C1 HA-33,
or a modified 2.gamma.-fold motif of a .beta.-trefoil domain of a
Clostridial botulinum serotype C1 HA-33 of SEQ ID NO: 16. In
another aspect of this embodiment, a .beta.-trefoil domain with
enhanced binding activity derived from a Clostridial botulinum
serotype C1 HA-33 comprises amino acids 10-54, amino acids 60-98,
amino acids 103-141, amino acids 148-190, amino acids 195-234, or
amino acids 241-285 of SEQ ID NO: 16.
[0425] In other aspects of this embodiment, a Clostridial botulinum
serotype C1 HA-33 comprising a .beta.-trefoil domain with enhanced
binding activity comprises a polypeptide having, e.g., at least 70%
amino acid identity with amino acids 10-54, amino acids 60-98,
amino acids 103-141, amino acids 148-190, amino acids 195-234, or
amino acids 241-285 of SEQ ID NO: 16, at least 75% amino acid
identity with amino acids 10-54, amino acids 60-98, amino acids
103-141, amino acids 148-190, amino acids 195-234, or amino acids
241-285 of SEQ ID NO: 16, at least 80% amino acid identity with
amino acids 10-54, amino acids 60-98, amino acids 103-141, amino
acids 148-190, amino acids 195-234, or amino acids 241-285 of SEQ
ID NO: 16, at least 85% amino acid identity with amino acids 10-54,
amino acids 60-98, amino acids 103-141, amino acids 148-190, amino
acids 195-234, or amino acids 241-285 of SEQ ID NO: 16, at least
90% amino acid identity with amino acids 10-54, amino acids 60-98,
amino acids 103-141, amino acids 148-190, amino acids 195-234, or
amino acids 241-285 of SEQ ID NO: 16 or at least 95% amino acid
identity with amino acids 10-54, amino acids 60-98, amino acids
103-141, amino acids 148-190, amino acids 195-234, or amino acids
241-285 of SEQ ID NO: 16. In yet other aspects of this embodiment,
a Clostridial botulinum serotype C1 HA-33 comprising a
.beta.-trefoil domain with enhanced binding activity comprises a
polypeptide having, e.g., at most 70% amino acid identity with
amino acids 10-54, amino acids 60-98, amino acids 103-141, amino
acids 148-190, amino acids 195-234, or amino acids 241-285 of SEQ
ID NO: 16, at most 75% amino acid identity with amino acids 10-54,
amino acids 60-98, amino acids 103-141, amino acids 148-190, amino
acids 195-234, or amino acids 241-285 of SEQ ID NO: 16, at most 80%
amino acid identity with amino acids 10-54, amino acids 60-98,
amino acids 103-141, amino acids 148-190, amino acids 195-234, or
amino acids 241-285 of SEQ ID NO: 16, at most 85% amino acid
identity with amino acids 10-54, amino acids 60-98, amino acids
103-141, amino acids 148-190, amino acids 195-234, or amino acids
241-285 of SEQ ID NO: 16, at most 90% amino acid identity with
amino acids 10-54, amino acids 60-98, amino acids 103-141, amino
acids 148-190, amino acids 195-234, or amino acids 241-285 of SEQ
ID NO: 16 or at most 95% amino acid identity with amino acids
10-54, amino acids 60-98, amino acids 103-141, amino acids 148-190,
amino acids 195-234, or amino acids 241-285 of SEQ ID NO: 16.
[0426] In other aspects of this embodiment, a Clostridial botulinum
serotype C1 HA-33 comprising a .beta.-trefoil domain with enhanced
binding activity comprises a polypeptide having, e.g., at most one,
two, three, four, five, six, seven, eight, nine, 10 or 20
non-contiguous amino acid substitutions relative to amino acids
10-54, amino acids 60-98, amino acids 103-141, amino acids 148-190,
amino acids 195-234, or amino acids 241-285 of SEQ ID NO: 16. In
other aspects of this embodiment, a Clostridial botulinum serotype
C1 HA-33 comprising a .beta.-trefoil domain with enhanced binding
activity comprises a polypeptide having, e.g., at least one, two,
three, four, five, six, seven, eight, nine, 10 or 20 non-contiguous
amino acid substitutions relative to amino acids 10-54, amino acids
60-98, amino acids 103-141, amino acids 148-190, amino acids
195-234, or amino acids 241-285 of SEQ ID NO: 16. In yet other
aspects of this embodiment, a Clostridial botulinum serotype C1
HA-33 comprising a .beta.-trefoil domain with enhanced binding
activity comprises a polypeptide having, e.g., at most one, two,
three, four, five, six, seven, eight, nine, 10 or 20 non-contiguous
amino acid deletions relative to amino acids 10-54, amino acids
60-98, amino acids 103-141, amino acids 148-190, amino acids
195-234, or amino acids 241-285 of SEQ ID NO: 16. In other aspects
of this embodiment, a Clostridial botulinum serotype C1 HA-33
comprising a .beta.-trefoil domain with enhanced binding activity
comprises a polypeptide having, e.g., at least one, two, three,
four, five, six, seven, eight, nine, 10 or 20 non-contiguous amino
acid deletions relative to amino acids 10-54, amino acids 60-98,
amino acids 103-141, amino acids 148-190, amino acids 195-234, or
amino acids 241-285 of SEQ ID NO: 16. In still other aspects of
this embodiment, a Clostridial botulinum serotype C1 HA-33
comprising a .beta.-trefoil domain with enhanced binding activity
comprises a polypeptide having, e.g., at most one, two, three,
four, five, six, seven, eight, nine, 10 or 20 non-contiguous amino
acid additions relative to amino acids 10-54, amino acids 60-98,
amino acids 103-141, amino acids 148-190, amino acids 195-234, or
amino acids 241-285 of SEQ ID NO: 16. In other aspects of this
embodiment, a Clostridial botulinum serotype C1 HA-33 comprising a
.beta.-trefoil domain with enhanced binding activity comprises a
polypeptide having, e.g., at least one, two, three, four, five,
six, seven, eight, nine, 10 or 20 non-contiguous amino acid
additions relative to amino acids 10-54, amino acids 60-98, amino
acids 103-141, amino acids 148-190, amino acids 195-234, or amino
acids 241-285 of SEQ ID NO: 16.
[0427] In other aspects of this embodiment, a Clostridial botulinum
serotype C1 HA-33 comprising a .beta.-trefoil domain with enhanced
binding activity comprises a polypeptide having, e.g., at most one,
two, three, four, five, six, seven, eight, nine, 10 or 20
contiguous amino acid substitutions relative to amino acids 10-54,
amino acids 60-98, amino acids 103-141, amino acids 148-190, amino
acids 195-234, or amino acids 241-285 of SEQ ID NO: 16. In other
aspects of this embodiment, a Clostridial botulinum serotype C1
HA-33 comprising a .beta.-trefoil domain with enhanced binding
activity comprises a polypeptide having, e.g., at least one, two,
three, four, five, six, seven, eight, nine, 10 or 20 contiguous
amino acid substitutions relative to amino acids 10-54, amino acids
60-98, amino acids 103-141, amino acids 148-190, amino acids
195-234, or amino acids 241-285 of SEQ ID NO: 16. In yet other
aspects of this embodiment, a Clostridial botulinum serotype C1
HA-33 comprising a .beta.-trefoil domain with enhanced binding
activity comprises a polypeptide having, e.g., at most one, two,
three, four, five, six, seven, eight, nine, 10 or 20 contiguous
amino acid deletions relative to amino acids 10-54, amino acids
60-98, amino acids 103-141, amino acids 148-190, amino acids
195-234, or amino acids 241-285 of SEQ ID NO: 16. In other aspects
of this embodiment, a Clostridial botulinum serotype C1 HA-33
comprising a .beta.-trefoil domain with enhanced binding activity
comprises a polypeptide having, e.g., at least one, two, three,
four, five, six, seven, eight, nine, 10 or 20 contiguous amino acid
deletions relative to amino acids 10-54, amino acids 60-98, amino
acids 103-141, amino acids 148-190, amino acids 195-234, or amino
acids 241-285 of SEQ ID NO: 16. In still other aspects of this
embodiment, a Clostridial botulinum serotype Cl HA-33 comprising a
.beta.-trefoil domain with enhanced binding activity comprises a
polypeptide having, e.g., at most one, two, three, four, five, six,
seven, eight, nine, 10 or 20 contiguous amino acid additions
relative to amino acids 10-54, amino acids 60-98, amino acids
103-141, amino acids 148-190, amino acids 195-234, or amino acids
241-285 of SEQ ID NO: 16. In other aspects of this embodiment, a
Clostridial botulinum serotype C1 HA-33 comprising a .beta.-trefoil
domain with enhanced binding activity comprises a polypeptide
having, e.g., at least one, two, three, four, five, six, seven,
eight, nine, 10 or 20 contiguous amino acid additions relative to
amino acids 10-54, amino acids 60-98, amino acids 103-141, amino
acids 148-190, amino acids 195-234, or amino acids 241-285 of SEQ
ID NO: 16.
[0428] In another embodiment, a .beta.-trefoil domain derived from
a Clostridial botulinum serotype C1 HA-33 comprises a
1.beta.4/.beta.5 hairpin turn of a .beta.-trefoil domain of a
Clostridial botulinum serotype C1 HA-33, a 1.beta.8/.beta.9 hairpin
turn of a .beta.-trefoil domain of a Clostridial botulinum serotype
C1 HA-33, a 2.beta.4/.beta.5 hairpin turn of a .beta.-trefoil
domain of a Clostridial botulinum serotype C1 HA-33 or a
2.beta.8/.beta.9 hairpin turn of a 13-trefoil domain of a
Clostridial botulinum serotype C1 HA-33 of SEQ ID NO: 16. In
another aspect of this embodiment, a .beta.-trefoil domain derived
from a Clostridial botulinum serotype C1 HA-33 comprises amino
acids 55-59, amino acids 99-102, amino acids 191-194, or amino
acids 235-240 of SEQ ID NO: 16.
[0429] In another embodiment, a .beta.-trefoil domain with enhanced
binding activity derived from a Clostridial botulinum serotype C1
HA-33 comprises a modified .beta.4/.beta.5 hairpin turn of a
.beta.-trefoil domain of a Clostridial botulinum serotype C1 HA-33
or a modified 1.beta.4/.beta.5 hairpin turn of a .beta.-trefoil
domain of a Clostridial botulinum serotype C1 HA-33, a modified
1.beta.8/.beta.9 hairpin turn of a .beta.-trefoil domain of a
Clostridial botulinum serotype C1 HA-33, a modified
2.beta.4/.beta.5 hairpin turn of a .beta.-trefoil domain of a
Clostridial botulinum serotype C1 HA-33 or a modified
2.beta.8/.beta.9 hairpin turn of a .beta.-trefoil domain of a
Clostridial botulinum serotype C1 HA-33 of SEQ ID NO: 16. In
another aspect of this embodiment, a .beta.-trefoil domain with
enhanced binding activity derived from a Clostridial botulinum
serotype C1 HA-33 comprises a modification of amino acids 55-59,
amino acids 99-102, amino acids 191-194, or amino acids 235-240 of
SEQ ID NO: 16.
[0430] In other aspects of this embodiment, a .beta.-trefoil domain
with enhanced binding activity derived from a Clostridial botulinum
serotype C1 HA-33 comprises a polypeptide having, e.g., at least
70% amino acid identity with amino acids 55-59, amino acids 99-102,
amino acids 191-194, or amino acids 235-240 of SEQ ID NO: 16, at
least 75% amino acid identity with amino acids 55-59, amino acids
99-102, amino acids 191-194, or amino acids 235-240 of SEQ ID NO:
16, at least 80% amino acid identity with amino acids 55-59, amino
acids 99-102, amino acids 191-194, or amino acids 235-240 of SEQ ID
NO: 16, at least 85% amino acid identity with amino acids 55-59,
amino acids 99-102, amino acids 191-194, or amino acids 235-240 of
SEQ ID NO: 16, at least 90% amino acid identity with amino acids
55-59, amino acids 99-102, amino acids 191-194, or amino acids
235-240 of SEQ ID NO: 16 or at least 95% amino acid identity with
amino acids 55-59, amino acids 99-102, amino acids 191-194, or
amino acids 235-240 of SEQ ID NO: 16. In yet other aspects of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a Clostridial botulinum serotype C1 HA-33 comprises a
polypeptide having, e.g., at most 70% amino acid identity with
amino acids 55-59, amino acids 99-102, amino acids 191-194, or
amino acids 235-240 of SEQ ID NO: 16, at most 75% amino acid
identity with amino acids 55-59, amino acids 99-102, amino acids
191-194, or amino acids 235-240 of SEQ ID NO: 16, at most 80% amino
acid identity with amino acids 55-59, amino acids 99-102, amino
acids 191-194, or amino acids 235-240 of SEQ ID NO: 16, at most 85%
amino acid identity with amino acids 55-59, amino acids 99-102,
amino acids 191-194, or amino acids 235-240 of SEQ ID NO: 16, at
most 90% amino acid identity with amino acids 55-59, amino acids
99-102, amino acids 191-194, or amino acids 235-240 of SEQ ID NO:
16 or at most 95% amino acid identity with amino acids 55-59, amino
acids 99-102, amino acids 191-194, or amino acids 235-240 of SEQ ID
NO: 16.
[0431] In other aspects of this embodiment, a .beta.-trefoil domain
with enhanced binding activity derived from a Clostridial botulinum
serotype C1 HA-33 comprises a polypeptide having, e.g., at most
one, two, three or four non-contiguous amino acid substitutions
relative to amino acids 55-59, amino acids 99-102, amino acids
191-194, or amino acids 235-240 of SEQ ID NO: 16. In other aspects
of this embodiment, a .beta.-trefoil domain with enhanced binding
activity derived from a Clostridial botulinum serotype C1 HA-33
comprises a polypeptide having, e.g., at least one, two, three or
four non-contiguous amino acid substitutions relative to amino
acids 55-59, amino acids 99-102, amino acids 191-194, or amino
acids 235-240 of SEQ ID NO: 16. In other aspects of this
embodiment, a non-contiguous amino acid substitution of any amino
acid from amino acids 55-59, amino acids 99-102, amino acids
191-194, or amino acids 235-240 of SEQ ID NO: 16 can be replaced
with glycine. In other aspects of this embodiment, a non-contiguous
amino acid substitution of any hydrophobic amino acid from amino
acids 55-59, amino acids 99-102, amino acids 191-194, or amino
acids 235-240 of SEQ ID NO: 16 can be replaced with phenylalanine.
In yet other aspects of this embodiment, a .beta.-trefoil domain
with enhanced binding activity derived from a Clostridial botulinum
serotype C1 HA-33 comprises a polypeptide having, e.g., at most
one, two, three or four non-contiguous amino acid deletions
relative to amino acids 55-59, amino acids 99-102, amino acids
191-194, or amino acids 235-240 of SEQ ID NO: 16. In other aspects
of this embodiment, a .beta.-trefoil domain with enhanced binding
activity derived from a Clostridial botulinum serotype C1 HA-33
comprises a polypeptide having, e.g., at least one, two, three or
four non-contiguous amino acid deletions relative to amino acids
55-59, amino acids 99-102, amino acids 191-194, or amino acids
235-240 of SEQ ID NO: 16. In still other aspects of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a Clostridial botulinum serotype C1 HA-33 comprises a
polypeptide having, e.g., at most one, two, three or four
non-contiguous amino acid additions relative to amino acids 55-59,
amino acids 99-102, amino acids 191-194, or amino acids 235-240 of
SEQ ID NO: 16. In other aspects of this embodiment, a
.beta.-trefoil domain with enhanced binding activity derived from a
Clostridial botulinum serotype C1 HA-33 comprises a polypeptide
having, e.g., at least one, two, three or four non-contiguous amino
acid additions relative to amino acids 55-59, amino acids 99-102,
amino acids 191-194, or amino acids 235-240 of SEQ ID NO: 16.
[0432] In other aspects of this embodiment, a .beta.-trefoil domain
with enhanced binding activity derived from a Clostridial botulinum
serotype C1 HA-33 comprises a polypeptide having, e.g., at most
one, two, three or four contiguous amino acid substitutions
relative to amino acids 55-59, amino acids 99-102, amino acids
191-194, or amino acids 235-240 of SEQ ID NO: 16. In other aspects
of this embodiment, a .beta.-trefoil domain with enhanced binding
activity derived from a Clostridial botulinum serotype C1 HA-33
comprises a polypeptide having, e.g., at least one, two, three or
four contiguous amino acid substitutions relative to amino acids
55-59, amino acids 99-102, amino acids 191-194, or amino acids
235-240 of SEQ ID NO: 16. In other aspects of this embodiment,
contiguous amino acid substitutions of amino acids from amino acids
55-59, amino acids 99-102, amino acids 191-194, or amino acids
235-240 of SEQ ID NO: 16 can be replaced with glycine. In other
aspects of this embodiment, contiguous amino acid substitutions of
hydrophobic amino acids from amino acids 55-59, amino acids 99-102,
amino acids 191-194, or amino acids 235-240 of SEQ ID NO: 16 can be
replaced with phenylalanine. In yet other aspects of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a Clostridial botulinum serotype C1 HA-33 comprises a
polypeptide having, e.g., at most one, two, three or four
contiguous amino acid deletions relative to amino acids 55-59,
amino acids 99-102, amino acids 191-194, or amino acids 235-240 of
SEQ ID NO: 16. In other aspects of this embodiment, a
.beta.-trefoil domain with enhanced binding activity derived from a
Clostridial botulinum serotype C1 HA-33 comprises a polypeptide
having, e.g., at least one, two, three or four contiguous amino
acid deletions relative to amino acids 55-59, amino acids 99-102,
amino acids 191-194, or amino acids 235-240 of SEQ ID NO: 16. In
still other aspects of this embodiment, a .beta.-trefoil domain
with enhanced binding activity derived from a Clostridial botulinum
serotype C1 HA-33 comprises a polypeptide having, e.g., at most
one, two, three or four contiguous amino acid additions relative to
amino acids 55-59, amino acids 99-102, amino acids 191-194, or
amino acids 235-240 of SEQ ID NO: 16. In other aspects of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a Clostridial botulinum serotype C1 HA-33 comprises a
polypeptide having, e.g., at least one, two, three or four
contiguous amino acid additions relative to amino acids 55-59,
amino acids 99-102, amino acids 191-194, or amino acids 235-240 of
SEQ ID NO: 16.
[0433] In another embodiment, a .beta.-trefoil domain derived from
a Clostridial botulinum serotype C1 HA-33 comprises a
.beta.-trefoil domain derived from a Clostridial botulinum serotype
C1 HA-33 of SEQ ID NO: 17. In another embodiment, a .beta.-trefoil
domain derived from a Clostridial botulinum serotype C1 HA-33
comprises amino acids 10-141 or amino acids 148-286 of SEQ ID NO:
17. In another aspect of this embodiment, a .beta.-trefoil domain
derived from a Clostridial botulinum serotype C1 HA-33 comprises a
la-fold motif of a .beta.-trefoil domain of a Clostridial botulinum
serotype C1 HA-33, a 1.beta.-fold motif of a .beta.-trefoil domain
of a Clostridial botulinum serotype C1 HA-33, a 1.gamma.-fold motif
of a .beta.-trefoil domain of a Clostridial botulinum serotype C1
HA-33, a 2.alpha.-fold motif of a .beta.-trefoil domain of a
Clostridial botulinum serotype Cl HA-33, a 2.beta.-fold motif of a
.beta.-trefoil domain of a Clostridial botulinum serotype C1 HA-33,
or a 2.gamma.-fold motif of a .beta.-trefoil domain of a
Clostridial botulinum serotype C1 HA-33 of SEQ ID NO: 17. In
another aspect of this embodiment, a .beta.-trefoil domain derived
from a Clostridial botulinum serotype C1 HA-33 comprises amino
acids 10-54, amino acids 60-98, amino acids 103-141, amino acids
148-190, amino acids 195-235, or amino acids 242-286 of SEQ ID NO:
17.
[0434] In another embodiment, a .beta.-trefoil domain with enhanced
binding activity derived from a Clostridial botulinum serotype C1
HA-33 comprises a .beta.-trefoil domain with enhanced binding
activity derived from a Clostridial botulinum serotype C1 HA-33 of
SEQ ID NO: 17. In another embodiment, a .beta.-trefoil domain with
enhanced binding activity derived from a Clostridial botulinum
serotype C1 HA-33 comprises amino acids 10-141 or amino acids
148-286 of SEQ ID NO: 17. In another aspect of this embodiment, a
.beta.-trefoil domain with enhanced binding activity derived from a
Clostridial botulinum serotype C1 HA-33 comprises a modified
1.alpha.-fold motif of a .beta.-trefoil domain of a Clostridial
botulinum serotype C1 HA-33, a modified 1.beta.-fold motif of a
.beta.-trefoil domain of a Clostridial botulinum serotype Cl HA-33,
a modified 1.gamma.-fold motif of a .beta.-trefoil domain of a
Clostridial botulinum serotype C1 HA-33, a modified 2.alpha.-fold
motif of a .beta.-trefoil domain of a Clostridial botulinum
serotype C1 HA-33, a modified 2.beta.-fold motif of a
.beta.-trefoil domain of a Clostridial botulinum serotype C1 HA-33,
or a modified 2.gamma.-fold motif of a .beta.-trefoil domain of a
Clostridial botulinum serotype C1 HA-33 of SEQ ID NO: 17. In
another aspect of this embodiment, a .beta.-trefoil domain with
enhanced binding activity derived from a Clostridial botulinum
serotype C1 HA-33 comprises amino acids 10-54, amino acids 60-98,
amino acids 103-141, amino acids 148-190, amino acids 195-235, or
amino acids 242-286 of SEQ ID NO: 17.
[0435] In other aspects of this embodiment, a Clostridial botulinum
serotype C1 HA-33 comprising a .beta.-trefoil domain with enhanced
binding activity comprises a polypeptide having, e.g., at least 70%
amino acid identity with amino acids 10-54, amino acids 60-98,
amino acids 103-141, amino acids 148-190, amino acids 195-235, or
amino acids 242-286 of SEQ ID NO: 17, at least 75% amino acid
identity with amino acids 10-54, amino acids 60-98, amino acids
103-141, amino acids 148-190, amino acids 195-235, or amino acids
242-286 of SEQ ID NO: 17, at least 80% amino acid identity with
amino acids 10-54, amino acids 60-98, amino acids 103-141, amino
acids 148-190, amino acids 195-235, or amino acids 242-286 of SEQ
ID NO: 17, at least 85% amino acid identity with amino acids 10-54,
amino acids 60-98, amino acids 103-141, amino acids 148-190, amino
acids 195-235, or amino acids 242-286 of SEQ ID NO: 17, at least
90% amino acid identity with amino acids 10-54, amino acids 60-98,
amino acids 103-141, amino acids 148-190, amino acids 195-235, or
amino acids 242-286 of SEQ ID NO: 17 or at least 95% amino acid
identity with amino acids 10-54, amino acids 60-98, amino acids
103-141, amino acids 148-190, amino acids 195-235, or amino acids
242-286 of SEQ ID NO: 17. In yet other aspects of this embodiment,
a Clostridial botulinum serotype C1 HA-33 comprising a
.beta.-trefoil domain with enhanced binding activity comprises a
polypeptide having, e.g., at most 70% amino acid identity with
amino acids 10-54, amino acids 60-98, amino acids 103-141, amino
acids 148-190, amino acids 195-235, or amino acids 242-286 of SEQ
ID NO: 17, at most 75% amino acid identity with amino acids 10-54,
amino acids 60-98, amino acids 103-141, amino acids 148-190, amino
acids 195-235, or amino acids 242-286 of SEQ ID NO: 17, at most 80%
amino acid identity with amino acids 10-54, amino acids 60-98,
amino acids 103-141, amino acids 148-190, amino acids 195-235, or
amino acids 242-286 of SEQ ID NO: 17, at most 85% amino acid
identity with amino acids 10-54, amino acids 60-98, amino acids
103-141, amino acids 148-190, amino acids 195-235, or amino acids
242-286 of SEQ ID NO: 17, at most 90% amino acid identity with
amino acids 10-54, amino acids 60-98, amino acids 103-141, amino
acids 148-190, amino acids 195-235, or amino acids 242-286 of SEQ
ID NO: 17 or at most 95% amino acid identity with amino acids
10-54, amino acids 60-98, amino acids 103-141, amino acids 148-190,
amino acids 195-235, or amino acids 242-286 of SEQ ID NO: 17.
[0436] In other aspects of this embodiment, a Clostridial botulinum
serotype C1 HA-33 comprising a 11-trefoil domain with enhanced
binding activity comprises a polypeptide having, e.g., at most one,
two, three, four, five, six, seven, eight, nine, 10 or 20
non-contiguous amino acid substitutions relative to amino acids
10-54, amino acids 60-98, amino acids 103-141, amino acids 148-190,
amino acids 195-235, or amino acids 242-286 of SEQ ID NO: 17. In
other aspects of this embodiment, a Clostridial botulinum serotype
C1 HA-33 comprising a .beta.-trefoil domain with enhanced binding
activity comprises a polypeptide having, e.g., at least one, two,
three, four, five, six, seven, eight, nine, 10 or 20 non-contiguous
amino acid substitutions relative to amino acids 10-54, amino acids
60-98, amino acids 103-141, amino acids 148-190, amino acids
195-235, or amino acids 242-286 of SEQ ID NO: 17. In yet other
aspects of this embodiment, a Clostridial botulinum serotype C1
HA-33 comprising a .beta.-trefoil domain with enhanced binding
activity comprises a polypeptide having, e.g., at most one, two,
three, four, five, six, seven, eight, nine, 10 or 20 non-contiguous
amino acid deletions relative to amino acids 10-54, amino acids
60-98, amino acids 103-141, amino acids 148-190, amino acids
195-235, or amino acids 242-286 of SEQ ID NO: 17. In other aspects
of this embodiment, a Clostridial botulinum serotype C1 HA-33
comprising a .beta.-trefoil domain with enhanced binding activity
comprises a polypeptide having, e.g., at least one, two, three,
four, five, six, seven, eight, nine, 10 or 20 non-contiguous amino
acid deletions relative to amino acids 10-54, amino acids 60-98,
amino acids 103-141, amino acids 148-190, amino acids 195-235, or
amino acids 242-286 of SEQ ID NO: 17. In still other aspects of
this embodiment, a Clostridial botulinum serotype C1 HA-33
comprising a .beta.-trefoil domain with enhanced binding activity
comprises a polypeptide having, e.g., at most one, two, three,
four, five, six, seven, eight, nine, 10 or 20 non-contiguous amino
acid additions relative to amino acids 10-54, amino acids 60-98,
amino acids 103-141, amino acids 148-190, amino acids 195-235, or
amino acids 242-286 of SEQ ID NO: 17. In other aspects of this
embodiment, a Clostridial botulinum serotype C1 HA-33 comprising a
.beta.-trefoil domain with enhanced binding activity comprises a
polypeptide having, e.g., at least one, two, three, four, five,
six, seven, eight, nine, 10 or 20 non-contiguous amino acid
additions relative to amino acids 10-54, amino acids 60-98, amino
acids 103-141, amino acids 148-190, amino acids 195-235, or amino
acids 242-286 of SEQ ID NO: 17.
[0437] In other aspects of this embodiment, a Clostridial botulinum
serotype C1 HA-33 comprising a .beta.-trefoil domain with enhanced
binding activity comprises a polypeptide having, e.g., at most one,
two, three, four, five, six, seven, eight, nine, 10 or 20
contiguous amino acid substitutions relative to amino acids 10-54,
amino acids 60-98, amino acids 103-141, amino acids 148-190, amino
acids 195-235, or amino acids 242-286 of SEQ ID NO: 17. In other
aspects of this embodiment, a Clostridial botulinum serotype C1
HA-33 comprising a .beta.-trefoil domain with enhanced binding
activity comprises a polypeptide having, e.g., at least one, two,
three, four, five, six, seven, eight, nine, 10 or 20 contiguous
amino acid substitutions relative to amino acids 10-54, amino acids
60-98, amino acids 103-141, amino acids 148-190, amino acids
195-235, or amino acids 242-286 of SEQ ID NO: 17. In yet other
aspects of this embodiment, a Clostridial botulinum serotype C1
HA-33 comprising a .beta.-trefoil domain with enhanced binding
activity comprises a polypeptide having, e.g., at most one, two,
three, four, five, six, seven, eight, nine, 10 or 20 contiguous
amino acid deletions relative to amino acids 10-54, amino acids
60-98, amino acids 103-141, amino acids 148-190, amino acids
195-235, or amino acids 242-286 of SEQ ID NO: 17. In other aspects
of this embodiment, a Clostridial botulinum serotype C1 HA-33
comprising a .beta.-trefoil domain with enhanced binding activity
comprises a polypeptide having, e.g., at least one, two, three,
four, five, six, seven, eight, nine, 10 or 20 contiguous amino acid
deletions relative to amino acids 10-54, amino acids 60-98, amino
acids 103-141, amino acids 148-190, amino acids 195-235, or amino
acids 242-286 of SEQ ID NO: 17. In still other aspects of this
embodiment, a Clostridial botulinum serotype Cl HA-33 comprising a
.beta.-trefoil domain with enhanced binding activity comprises a
polypeptide having, e.g., at most one, two, three, four, five, six,
seven, eight, nine, 10 or 20 contiguous amino acid additions
relative to amino acids 10-54, amino acids 60-98, amino acids
103-141, amino acids 148-190, amino acids 195-235, or amino acids
242-286 of SEQ ID NO: 17. In other aspects of this embodiment, a
Clostridial botulinum serotype C1 HA-33 comprising a .beta.-trefoil
domain with enhanced binding activity comprises a polypeptide
having, e.g., at least one, two, three, four, five, six, seven,
eight, nine, 10 or 20 contiguous amino acid additions relative to
amino acids 10-54, amino acids 60-98, amino acids 103-141, amino
acids 148-190, amino acids 195-235, or amino acids 242-286 of SEQ
ID NO: 17.
[0438] In another embodiment, a .beta.-trefoil domain derived from
a Clostridial botulinum serotype C1 HA-33 comprises a
1.beta.4/.beta.5 hairpin turn of a .beta.-trefoil domain of a
Clostridial botulinum serotype C1 HA-33, a 1.beta.8/.beta.9 hairpin
turn of a .beta.-trefoil domain of a Clostridial botulinum serotype
C1 HA-33, a 2.beta.4/.beta.5 hairpin turn of a .beta.-trefoil
domain of a Clostridial botulinum serotype C1 HA-33 or a
2.beta.8/.beta.9 hairpin turn of a 13-trefoil domain of a
Clostridial botulinum serotype C1 HA-33 of SEQ ID NO: 17. In
another aspect of this embodiment, a .beta.-trefoil domain derived
from a Clostridial botulinum serotype C1 HA-33 comprises amino
acids 55-59, amino acids 99-102, amino acids 191-194, or amino
acids 236-241 of SEQ ID NO: 17.
[0439] In another embodiment, a .beta.-trefoil domain with enhanced
binding activity derived from a Clostridial botulinum serotype C1
HA-33 comprises a modified 1.beta.4/.beta.5 hairpin turn of a
.beta.-trefoil domain of a Clostridial botulinum serotype C1 HA-33,
a modified 1.beta.8/.beta.9 hairpin turn of a .beta.-trefoil domain
of a Clostridial botulinum serotype C1 HA-33, a modified
2.beta.4/.beta.5 hairpin turn of a .beta.-trefoil domain of a
Clostridial botulinum serotype C1 HA-33 or a modified
2.beta.8/.beta.9 hairpin turn of a .beta.-trefoil domain of a
Clostridial botulinum serotype C1 HA-33 of SEQ ID NO: 17. In
another aspect of this embodiment, a R-trefoil domain with enhanced
binding activity derived from a Clostridial botulinum serotype C1
HA-33 comprises a modification of amino acids 55-59, amino acids
99-102, amino acids 191-194, or amino acids 236-241 of SEQ ID NO:
17.
[0440] In other aspects of this embodiment, a .beta.-trefoil domain
with enhanced binding activity derived from a Clostridial botulinum
serotype C1 HA-33 comprises a polypeptide having, e.g., at least
70% amino acid identity with amino acids 55-59, amino acids 99-102,
amino acids 191-194, or amino acids 236-241 of SEQ ID NO: 17, at
least 75% amino acid identity with amino acids 55-59, amino acids
99-102, amino acids 191-194, or amino acids 236-241 of SEQ ID NO:
17, at least 80% amino acid identity with amino acids 55-59, amino
acids 99-102, amino acids 191-194, or amino acids 236-241 of SEQ ID
NO: 17, at least 85% amino acid identity with amino acids 55-59,
amino acids 99-102, amino acids 191-194, or amino acids 236-241 of
SEQ ID NO: 17, at least 90% amino acid identity with amino acids
55-59, amino acids 99-102, amino acids 191-194, or amino acids
236-241 of SEQ ID NO: 17 or at least 95% amino acid identity with
amino acids 55-59, amino acids 99-102, amino acids 191-194, or
amino acids 236-241 of SEQ ID NO: 17. In yet other aspects of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a Clostridial botulinum serotype C1 HA-33 comprises a
polypeptide having, e.g., at most 70% amino acid identity with
amino acids 55-59, amino acids 99-102, amino acids 191-194, or
amino acids 236-241 of SEQ ID NO: 17, at most 75% amino acid
identity with amino acids 55-59, amino acids 99-102, amino acids
191-194, or amino acids 236-241 of SEQ ID NO: 17, at most 80% amino
acid identity with amino acids 55-59, amino acids 99-102, amino
acids 191-194, or amino acids 236-241 of SEQ ID NO: 17, at most 85%
amino acid identity with amino acids 55-59, amino acids 99-102,
amino acids 191-194, or amino acids 236-241 of SEQ ID NO: 17, at
most 90% amino acid identity with amino acids 55-59, amino acids
99-102, amino acids 191-194, or amino acids 236-241 of SEQ ID NO:
17 or at most 95% amino acid identity with amino acids 55-59, amino
acids 99-102, amino acids 191-194, or amino acids 236-241 of SEQ ID
NO: 17.
[0441] In other aspects of this embodiment, a .beta.-trefoil domain
with enhanced binding activity derived from a Clostridial botulinum
serotype C1 HA-33 comprises a polypeptide having, e.g., at most
one, two, three or four non-contiguous amino acid substitutions
relative to amino acids 55-59, amino acids 99-102, amino acids
191-194, or amino acids 236-241 of SEQ ID NO: 17. In other aspects
of this embodiment, a .beta.-trefoil domain with enhanced binding
activity derived from a Clostridial botulinum serotype C1 HA-33
comprises a polypeptide having, e.g., at least one, two, three or
four non-contiguous amino acid substitutions relative to amino
acids 55-59, amino acids 99-102, amino acids 191-194, or amino
acids 236-241 of SEQ ID NO: 17. In other aspects of this
embodiment, a non-contiguous amino acid substitution of any amino
acid from amino acids 55-59, amino acids 99-102, amino acids
191-194, or amino acids 236-241 of SEQ ID NO: 17 can be replaced
with glycine. In other aspects of this embodiment, a non-contiguous
amino acid substitution of any hydrophobic amino acid from amino
acids 55-59, amino acids 99-102, amino acids 191-194, or amino
acids 236-241 of SEQ ID NO: 17 can be replaced with phenylalanine.
In yet other aspects of this embodiment, a .beta.-trefoil domain
with enhanced binding activity derived from a Clostridial botulinum
serotype C1 HA-33 comprises a polypeptide having, e.g., at most
one, two, three or four non-contiguous amino acid deletions
relative to amino acids 55-59, amino acids 99-102, amino acids
191-194, or amino acids 236-241 of SEQ ID NO: 17. In other aspects
of this embodiment, a .beta.-trefoil domain with enhanced binding
activity derived from a Clostridial botulinum serotype C1 HA-33
comprises a polypeptide having, e.g., at least one, two, three or
four non-contiguous amino acid deletions relative to amino acids
55-59, amino acids 99-102, amino acids 191-194, or amino acids
236-241 of SEQ ID NO: 17. In still other aspects of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a Clostridial botulinum serotype C1 HA-33 comprises a
polypeptide having, e.g., at most one, two, three or four
non-contiguous amino acid additions relative to amino acids 55-59,
amino acids 99-102, amino acids 191-194, or amino acids 236-241 of
SEQ ID NO: 17. In other aspects of this embodiment, a
.beta.-trefoil domain with enhanced binding activity derived from a
Clostridial botulinum serotype C1 HA-33 comprises a polypeptide
having, e.g., at least one, two, three or four non-contiguous amino
acid additions relative to amino acids 55-59, amino acids 99-102,
amino acids 191-194, or amino acids 236-241 of SEQ ID NO: 17.
[0442] In other aspects of this embodiment, a .beta.-trefoil domain
with enhanced binding activity derived from a Clostridial botulinum
serotype C1 HA-33 comprises a polypeptide having, e.g., at most
one, two, three or four contiguous amino acid substitutions
relative to amino acids 55-59, amino acids 99-102, amino acids
191-194, or amino acids 236-241 of SEQ ID NO: 17. In other aspects
of this embodiment, a .beta.-trefoil domain with enhanced binding
activity derived from a Clostridial botulinum serotype C1 HA-33
comprises a polypeptide having, e.g., at least one, two, three or
four contiguous amino acid substitutions relative to amino acids
55-59, amino acids 99-102, amino acids 191-194, or amino acids
236-241 of SEQ ID NO: 17. In other aspects of this embodiment,
contiguous amino acid substitutions of amino acids from amino acids
55-59, amino acids 99-102, amino acids 191-194, or amino acids
236-241 of SEQ ID NO: 17 can be replaced with glycine. In other
aspects of this embodiment, contiguous amino acid substitutions of
hydrophobic amino acids from amino acids 55-59, amino acids 99-102,
amino acids 191-194, or amino acids 236-241 of SEQ ID NO: 17 can be
replaced with phenylalanine. In yet other aspects of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a Clostridial botulinum serotype C1 HA-33 comprises a
polypeptide having, e.g., at most one, two, three or four
contiguous amino acid deletions relative to amino acids 55-59,
amino acids 99-102, amino acids 191-194, or amino acids 236-241 of
SEQ ID NO: 17. In other aspects of this embodiment, a
.beta.-trefoil domain with enhanced binding activity derived from a
Clostridial botulinum serotype C1 HA-33 comprises a polypeptide
having, e.g., at least one, two, three or four contiguous amino
acid deletions relative to amino acids 55-59, amino acids 99-102,
amino acids 191-194, or amino acids 236-241 of SEQ ID NO: 17. In
still other aspects of this embodiment, a .beta.-trefoil domain
with enhanced binding activity derived from a Clostridial botulinum
serotype C1 HA-33 comprises a polypeptide having, e.g., at most
one, two, three or four contiguous amino acid additions relative to
amino acids 55-59, amino acids 99-102, amino acids 191-194, or
amino acids 236-241 of SEQ ID NO: 17. In other aspects of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a Clostridial botulinum serotype C1 HA-33 comprises a
polypeptide having, e.g., at least one, two, three or four
contiguous amino acid additions relative to amino acids 55-59,
amino acids 99-102, amino acids 191-194, or amino acids 236-241 of
SEQ ID NO: 17.
[0443] In another embodiment, a .beta.-trefoil domain derived from
a Clostridial botulinum serotype D HA-33 comprises a .beta.-trefoil
domain derived from a Clostridial botulinum serotype D HA-33 of SEQ
ID NO: 18. In another embodiment, a .beta.-trefoil domain derived
from a Clostridial botulinum serotype D HA-33 comprises amino acids
10-141 or amino acids 148-286 of SEQ ID NO: 18. In another aspect
of this embodiment, a .beta.-trefoil domain derived from a
Clostridial botulinum serotype D HA-33 comprises a 1.alpha.-fold
motif of a 13-trefoil domain of a Clostridial botulinum serotype D
HA-33, a 1.beta.-fold motif of a .beta.-trefoil domain of a
Clostridial botulinum serotype D HA-33, a 1.gamma.-fold motif of a
.beta.-trefoil domain of a Clostridial botulinum serotype D HA-33,
a 2.alpha.-fold motif of a .beta.-trefoil domain of a Clostridial
botulinum serotype D HA-33, a 2.beta.-fold motif of a
.beta.-trefoil domain of a Clostridial botulinum serotype D HA-33,
or a 2.gamma.-fold motif of a .beta.-trefoil domain of a
Clostridial botulinum serotype D HA-33 of SEQ ID NO: 18. In another
aspect of this embodiment, a .beta.-trefoil domain derived from a
Clostridial botulinum serotype D HA-33 comprises amino acids 10-54,
amino acids 60-98, amino acids 103-141, amino acids 148-190, amino
acids 195-235, or amino acids 242-286 of SEQ ID NO: 18.
[0444] In another embodiment, a .beta.-trefoil domain with enhanced
binding activity derived from a Clostridial botulinum serotype D
HA-33 comprises a .beta.-trefoil domain with enhanced binding
activity derived from a Clostridial botulinum serotype D HA-33 of
SEQ ID NO: 18. In another embodiment, a .beta.-trefoil domain with
enhanced binding activity derived from a Clostridial botulinum
serotype D HA-33 comprises amino acids 10-141 or amino acids
148-286 of SEQ ID NO: 18. In another aspect of this embodiment, a
.beta.-trefoil domain with enhanced binding activity derived from a
Clostridial botulinum serotype D HA-33 comprises a modified
1.alpha.-fold motif of a .beta.-trefoil domain of a Clostridial
botulinum serotype D HA-33, a modified 1.beta.-fold motif of a
.beta.-trefoil domain of a Clostridial botulinum serotype D HA-33,
a modified 1.gamma.-fold motif of a .beta.-trefoil domain of a
Clostridial botulinum serotype D HA-33, a modified 2.alpha.-fold
motif of a .beta.-trefoil domain of a Clostridial botulinum
serotype D HA-33, a modified 2.beta.-fold motif of a .beta.-trefoil
domain of a Clostridial botulinum serotype D HA-33, or a modified
2.gamma.-fold motif of a .beta.-trefoil domain of a Clostridial
botulinum serotype D HA-33 of SEQ ID NO: 18. In another aspect of
this embodiment, a .beta.-trefoil domain with enhanced binding
activity derived from a Clostridial botulinum serotype D HA-33
comprises amino acids 10-54, amino acids 60-98, amino acids
103-141, amino acids 148-190, amino acids 195-235, or amino acids
242-286 of SEQ ID NO: 18.
[0445] In other aspects of this embodiment, a Clostridial botulinum
serotype D HA-33 comprising a .beta.-trefoil domain with enhanced
binding activity comprises a polypeptide having, e.g., at least 70%
amino acid identity with amino acids 10-54, amino acids 60-98,
amino acids 103-141, amino acids 148-190, amino acids 195-235, or
amino acids 242-286 of SEQ ID NO: 18, at least 75% amino acid
identity with amino acids 10-54, amino acids 60-98, amino acids
103-141, amino acids 148-190, amino acids 195-235, or amino acids
242-286 of SEQ ID NO: 18, at least 80% amino acid identity with
amino acids 10-54, amino acids 60-98, amino acids 103-141, amino
acids 148-190, amino acids 195-235, or amino acids 242-286 of SEQ
ID NO: 18, at least 85% amino acid identity with amino acids 10-54,
amino acids 60-98, amino acids 103-141, amino acids 148-190, amino
acids 195-235, or amino acids 242-286 of SEQ ID NO: 18, at least
90% amino acid identity with amino acids 10-54, amino acids 60-98,
amino acids 103-141, amino acids 148-190, amino acids 195-235, or
amino acids 242-286 of SEQ ID NO: 18 or at least 95% amino acid
identity with amino acids 10-54, amino acids 60-98, amino acids
103-141, amino acids 148-190, amino acids 195-235, or amino acids
242-286 of SEQ ID NO: 18. In yet other aspects of this embodiment,
a Clostridial botulinum serotype D HA-33 comprising a
.beta.-trefoil domain with enhanced binding activity comprises a
polypeptide having, e.g., at most 70% amino acid identity with
amino acids 10-54, amino acids 60-98, amino acids 103-141, amino
acids 148-190, amino acids 195-235, or amino acids 242-286 of SEQ
ID NO: 18, at most 75% amino acid identity with amino acids 10-54,
amino acids 60-98, amino acids 103-141, amino acids 148-190, amino
acids 195-235, or amino acids 242-286 of SEQ ID NO: 18, at most 80%
amino acid identity with amino acids 10-54, amino acids 60-98,
amino acids 103-141, amino acids 148-190, amino acids 195-235, or
amino acids 242-286 of SEQ ID NO: 18, at most 85% amino acid
identity with amino acids 10-54, amino acids 60-98, amino acids
103-141, amino acids 148-190, amino acids 195-235, or amino acids
242-286 of SEQ ID NO: 18, at most 90% amino acid identity with
amino acids 10-54, amino acids 60-98, amino acids 103-141, amino
acids 148-190, amino acids 195-235, or amino acids 242-286 of SEQ
ID NO: 18 or at most 95% amino acid identity with amino acids
10-54, amino acids 60-98, amino acids 103-141, amino acids 148-190,
amino acids 195-235, or amino acids 242-286 of SEQ ID NO: 18.
[0446] In other aspects of this embodiment, a Clostridial botulinum
serotype D HA-33 comprising a .beta.-trefoil domain with enhanced
binding activity comprises a polypeptide having, e.g., at most one,
two, three, four, five, six, seven, eight, nine, 10 or 20
non-contiguous amino acid substitutions relative to amino acids
10-54, amino acids 60-98, amino acids 103-141, amino acids 148-190,
amino acids 195-235, or amino acids 242-286 of SEQ ID NO: 18. In
other aspects of this embodiment, a Clostridial botulinum serotype
D HA-33 comprising a .beta.-trefoil domain with enhanced binding
activity comprises a polypeptide having, e.g., at least one, two,
three, four, five, six, seven, eight, nine, 10 or 20 non-contiguous
amino acid substitutions relative to amino acids 10-54, amino acids
60-98, amino acids 103-141, amino acids 148-190, amino acids
195-235, or amino acids 242-286 of SEQ ID NO: 18. In yet other
aspects of this embodiment, a Clostridial botulinum serotype D
HA-33 comprising a .beta.-trefoil domain with enhanced binding
activity comprises a polypeptide having, e.g., at most one, two,
three, four, five, six, seven, eight, nine, 10 or 20 non-contiguous
amino acid deletions relative to amino acids 10-54, amino acids
60-98, amino acids 103-141, amino acids 148-190, amino acids
195-235, or amino acids 242-286 of SEQ ID NO: 18. In other aspects
of this embodiment, a Clostridial botulinum serotype D HA-33
comprising a .beta.-trefoil domain with enhanced binding activity
comprises a polypeptide having, e.g., at least one, two, three,
four, five, six, seven, eight, nine, 10 or 20 non-contiguous amino
acid deletions relative to amino acids 10-54, amino acids 60-98,
amino acids 103-141, amino acids 148-190, amino acids 195-235, or
amino acids 242-286 of SEQ ID NO: 18. In still other aspects of
this embodiment, a Clostridial botulinum serotype D HA-33
comprising a .beta.-trefoil domain with enhanced binding activity
comprises a polypeptide having, e.g., at most one, two, three,
four, five, six, seven, eight, nine, 10 or 20 non-contiguous amino
acid additions relative to amino acids 10-54, amino acids 60-98,
amino acids 103-141, amino acids 148-190, amino acids 195-235, or
amino acids 242-286 of SEQ ID NO: 18. In other aspects of this
embodiment, a Clostridial botulinum serotype D HA-33 comprising a
.beta.-trefoil domain with enhanced binding activity comprises a
polypeptide having, e.g., at least one, two, three, four, five,
six, seven, eight, nine, 10 or 20 non-contiguous amino acid
additions relative to amino acids 10-54, amino acids 60-98, amino
acids 103-141, amino acids 148-190, amino acids 195-235, or amino
acids 242-286 of SEQ ID NO: 18.
[0447] In other aspects of this embodiment, a Clostridial botulinum
serotype D HA-33 comprising a .beta.-trefoil domain with enhanced
binding activity comprises a polypeptide having, e.g., at most one,
two, three, four, five, six, seven, eight, nine, 10 or 20
contiguous amino acid substitutions relative to amino acids 10-54,
amino acids 60-98, amino acids 103-141, amino acids 148-190, amino
acids 195-235, or amino acids 242-286 of SEQ ID NO: 18. In other
aspects of this embodiment, a Clostridial botulinum serotype D
HA-33 comprising a .beta.-trefoil domain with enhanced binding
activity comprises a polypeptide having, e.g., at least one, two,
three, four, five, six, seven, eight, nine, 10 or 20 contiguous
amino acid substitutions relative to amino acids 10-54, amino acids
60-98, amino acids 103-141, amino acids 148-190, amino acids
195-235, or amino acids 242-286 of SEQ ID NO: 18. In yet other
aspects of this embodiment, a Clostridial botulinum serotype D
HA-33 comprising a .beta.-trefoil domain with enhanced binding
activity comprises a polypeptide having, e.g., at most one, two,
three, four, five, six, seven, eight, nine, 10 or 20 contiguous
amino acid deletions relative to amino acids 10-54, amino acids
60-98, amino acids 103-141, amino acids 148-190, amino acids
195-235, or amino acids 242-286 of SEQ ID NO: 18. In other aspects
of this embodiment, a Clostridial botulinum serotype D HA-33
comprising a .beta.-trefoil domain with enhanced binding activity
comprises a polypeptide having, e.g., at least one, two, three,
four, five, six, seven, eight, nine, 10 or 20 contiguous amino acid
deletions relative to amino acids 10-54, amino acids 60-98, amino
acids 103-141, amino acids 148-190, amino acids 195-235, or amino
acids 242-286 of SEQ ID NO: 18. In still other aspects of this
embodiment, a Clostridial botulinum serotype D HA-33 comprising a
.beta.-trefoil domain with enhanced binding activity comprises a
polypeptide having, e.g., at most one, two, three, four, five, six,
seven, eight, nine, 10 or 20 contiguous amino acid additions
relative to amino acids 10-54, amino acids 60-98, amino acids
103-141, amino acids 148-190, amino acids 195-235, or amino acids
242-286 of SEQ ID NO: 18. In other aspects of this embodiment, a
Clostridial botulinum serotype D HA-33 comprising a .beta.-trefoil
domain with enhanced binding activity comprises a polypeptide
having, e.g., at least one, two, three, four, five, six, seven,
eight, nine, 10 or 20 contiguous amino acid additions relative to
amino acids 10-54, amino acids 60-98, amino acids 103-141, amino
acids 148-190, amino acids 195-235, or amino acids 242-286 of SEQ
ID NO: 18.
[0448] In another embodiment, a .beta.-trefoil domain derived from
a Clostridial botulinum serotype D HA-33 comprises a
1.beta.4/.beta.5 hairpin turn of a .beta.-trefoil domain of a
Clostridial botulinum serotype D HA-33, a 1.beta.8/.beta.9 hairpin
turn of a .beta.-trefoil domain of a Clostridial botulinum serotype
D HA-33, a 2.beta.4/.beta.5 hairpin turn of a .beta.-trefoil domain
of a Clostridial botulinum serotype D HA-33 or a 2.beta.8/.beta.9
hairpin turn of a .beta.-trefoil domain of a Clostridial botulinum
serotype D HA-33 of SEQ ID NO: 18. In another aspect of this
embodiment, a .beta.-trefoil domain derived from a Clostridial
botulinum serotype D HA-33 comprises amino acids 55-59, amino acids
99-102, amino acids 191-194, or amino acids 236-241 of SEQ ID NO:
18.
[0449] In another embodiment, a .beta.-trefoil domain with enhanced
binding activity derived from a Clostridial botulinum serotype D
HA-33 comprises a modified 1.beta.4/.beta.5 hairpin turn of a
.beta.-trefoil domain of a Clostridial botulinum serotype D HA-33,
a modified 1.beta.8/.beta.9 hairpin turn of a .beta.-trefoil domain
of a Clostridial botulinum serotype D HA-33, a modified
2.beta.4/.beta.5 hairpin turn of a .beta.-trefoil domain of a
Clostridial botulinum serotype D HA-33 or a modified
2.beta.8/.beta.9 hairpin turn of a .beta.-trefoil domain of a
Clostridial botulinum serotype D HA-33 of SEQ ID NO: 18. In another
aspect of this embodiment, a .beta.-trefoil domain with enhanced
binding activity derived from a Clostridial botulinum serotype D
HA-33 comprises a modification of amino acids 55-59, amino acids
99-102, amino acids 191-194, or amino acids 236-241 of SEQ ID NO:
18.
[0450] In other aspects of this embodiment, a .beta.-trefoil domain
with enhanced binding activity derived from a Clostridial botulinum
serotype D HA-33 comprises a polypeptide having, e.g., at least 70%
amino acid identity with amino acids 55-59, amino acids 99-102,
amino acids 191-194, or amino acids 236-241 of SEQ ID NO: 18, at
least 75% amino acid identity with amino acids 55-59, amino acids
99-102, amino acids 191-194, or amino acids 236-241 of SEQ ID NO:
18, at least 80% amino acid identity with amino acids 55-59, amino
acids 99-102, amino acids 191-194, or amino acids 236-241 of SEQ ID
NO: 18, at least 85% amino acid identity with amino acids 55-59,
amino acids 99-102, amino acids 191-194, or amino acids 236-241 of
SEQ ID NO: 18, at least 90% amino acid identity with amino acids
55-59, amino acids 99-102, amino acids 191-194, or amino acids
236-241 of SEQ ID NO: 18 or at least 95% amino acid identity with
amino acids 55-59, amino acids 99-102, amino acids 191-194, or
amino acids 236-241 of SEQ ID NO: 18. In yet other aspects of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a Clostridial botulinum serotype D HA-33 comprises a
polypeptide having, e.g., at most 70% amino acid identity with
amino acids 55-59, amino acids 99-102, amino acids 191-194, or
amino acids 236-241 of SEQ ID NO: 18, at most 75% amino acid
identity with amino acids 55-59, amino acids 99-102, amino acids
191-194, or amino acids 236-241 of SEQ ID NO: 18, at most 80% amino
acid identity with amino acids 55-59, amino acids 99-102, amino
acids 191-194, or amino acids 236-241 of SEQ ID NO: 18, at most 85%
amino acid identity with amino acids 55-59, amino acids 99-102,
amino acids 191-194, or amino acids 236-241 of SEQ ID NO: 18, at
most 90% amino acid identity with amino acids 55-59, amino acids
99-102, amino acids 191-194, or amino acids 236-241 of SEQ ID NO:
18 or at most 95% amino acid identity with amino acids 55-59, amino
acids 99-102, amino acids 191-194, or amino acids 236-241 of SEQ ID
NO: 18.
[0451] In other aspects of this embodiment, a .beta.-trefoil domain
with enhanced binding activity derived from a Clostridial botulinum
serotype D HA-33 comprises a polypeptide having, e.g., at most one,
two, three or four non-contiguous amino acid substitutions relative
to amino acids 55-59, amino acids 99-102, amino acids 191-194, or
amino acids 236-241 of SEQ ID NO: 18. In other aspects of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a Clostridial botulinum serotype D HA-33 comprises a
polypeptide having, e.g., at least one, two, three or four
non-contiguous amino acid substitutions relative to amino acids
55-59, amino acids 99-102, amino acids 191-194, or amino acids
236-241 of SEQ ID NO: 18. In other aspects of this embodiment, a
non-contiguous amino acid substitution of any amino acid from amino
acids 55-59, amino acids 99-102, amino acids 191-194, or amino
acids 236-241 of SEQ ID NO: 18 can be replaced with glycine. In
other aspects of this embodiment, a non-contiguous amino acid
substitution of any hydrophobic amino acid from amino acids 55-59,
amino acids 99-102, amino acids 191-194, or amino acids 236-241 of
SEQ ID NO: 18 can be replaced with phenylalanine. In yet other
aspects of this embodiment, a .beta.-trefoil domain with enhanced
binding activity derived from a Clostridial botulinum serotype D
HA-33 comprises a polypeptide having, e.g., at most one, two, three
or four non-contiguous amino acid deletions relative to amino acids
55-59, amino acids 99-102, amino acids 191-194, or amino acids
236-241 of SEQ ID NO: 18. In other aspects of this embodiment, a
.beta.-trefoil domain with enhanced binding activity derived from a
Clostridial botulinum serotype D HA-33 comprises a polypeptide
having, e.g., at least one, two, three or four non-contiguous amino
acid deletions relative to amino acids 55-59, amino acids 99-102,
amino acids 191-194, or amino acids 236-241 of SEQ ID NO: 18. In
still other aspects of this embodiment, a .beta.-trefoil domain
with enhanced binding activity derived from a Clostridial botulinum
serotype D HA-33 comprises a polypeptide having, e.g., at most one,
two, three or four non-contiguous amino acid additions relative to
amino acids 55-59, amino acids 99-102, amino acids 191-194, or
amino acids 236-241 of SEQ ID NO: 18. In other aspects of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a Clostridial botulinum serotype D HA-33 comprises a
polypeptide having, e.g., at least one, two, three or four
non-contiguous amino acid additions relative to amino acids 55-59,
amino acids 99-102, amino acids 191-194, or amino acids 236-241 of
SEQ ID NO: 18.
[0452] In other aspects of this embodiment, a .beta.-trefoil domain
with enhanced binding activity derived from a Clostridial botulinum
serotype D HA-33 comprises a polypeptide having, e.g., at most one,
two, three or four contiguous amino acid substitutions relative to
amino acids 55-59, amino acids 99-102, amino acids 191-194, or
amino acids 236-241 of SEQ ID NO: 18. In other aspects of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a Clostridial botulinum serotype D HA-33 comprises a
polypeptide having, e.g., at least one, two, three or four
contiguous amino acid substitutions relative to amino acids 55-59,
amino acids 99-102, amino acids 191-194, or amino acids 236-241 of
SEQ ID NO: 18. In other aspects of this embodiment, contiguous
amino acid substitutions of amino acids from amino acids 55-59,
amino acids 99-102, amino acids 191-194, or amino acids 236-241 of
SEQ ID NO: 18 can be replaced with glycine. In other aspects of
this embodiment, contiguous amino acid substitutions of hydrophobic
amino acids from amino acids 55-59, amino acids 99-102, amino acids
191-194, or amino acids 236-241 of SEQ ID NO: 18 can be replaced
with phenylalanine. In yet other aspects of this embodiment, a
.beta.-trefoil domain with enhanced binding activity derived from a
Clostridial botulinum serotype D HA-33 comprises a polypeptide
having, e.g., at most one, two, three or four contiguous amino acid
deletions relative to amino acids 55-59, amino acids 99-102, amino
acids 191-194, or amino acids 236-241 of SEQ ID NO: 18. In other
aspects of this embodiment, a .beta.-trefoil domain with enhanced
binding activity derived from a Clostridial botulinum serotype D
HA-33 comprises a polypeptide having, e.g., at least one, two,
three or four contiguous amino acid deletions relative to amino
acids 55-59, amino acids 99-102, amino acids 191-194, or amino
acids 236-241 of SEQ ID NO: 18. In still other aspects of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a Clostridial botulinum serotype D HA-33 comprises a
polypeptide having, e.g., at most one, two, three or four
contiguous amino acid additions relative to amino acids 55-59,
amino acids 99-102, amino acids 191-194, or amino acids 236-241 of
SEQ ID NO: 18. In other aspects of this embodiment, a
.beta.-trefoil domain with enhanced binding activity derived from a
Clostridial botulinum serotype D HA-33 comprises a polypeptide
having, e.g., at least one, two, three or four contiguous amino
acid additions relative to amino acids 55-59, amino acids 99-102,
amino acids 191-194, or amino acids 236-241 of SEQ ID NO: 18.
[0453] In an embodiment, a modified Clostridial toxin disclosed in
the present specification comprises an enhanced targeting domain
comprising a .beta.-trefoil domain derived from a Clostridial
HA-17. In an aspect of this embodiment, a .beta.-trefoil domain
derived from a Clostridial HA-17 comprises, e.g., a .beta.-trefoil
domain derived from a Clostridial botulinum serotype A HA-17, a
.beta.-trefoil domain derived from a Clostridial botulinum serotype
B HA-17, a .beta.-trefoil domain derived from a Clostridial
botulinum serotype C1 HA-17 or a .beta.-trefoil domain derived from
a Clostridial botulinum serotype D HA-17. In another aspect of this
embodiment, a .beta.-trefoil domain derived from a Clostridial
HA-17 comprises a .alpha.-fold motif of a .beta.-trefoil domain of
a Clostridial HA-17, a .beta.-fold motif of a .beta.-trefoil domain
of a Clostridial HA-17 or a .gamma.-fold motif of a .beta.-trefoil
domain of a Clostridial HA-17.
[0454] In an embodiment, a modified Clostridial toxin disclosed in
the present specification comprises an enhanced targeting domain
comprising a .beta.-trefoil domain with enhanced binding activity
derived from a Clostridial HA-17. In an aspect of this embodiment,
a .beta.-trefoil domain with enhanced binding activity derived from
a Clostridial HA-17 comprises, e.g., a .beta.-trefoil domain with
enhanced binding activity derived from a Clostridial botulinum
serotype A HA-17, a .beta.-trefoil domain with enhanced binding
activity derived from a Clostridial botulinum serotype B HA-17, a
.beta.-trefoil domain with enhanced binding activity derived from a
Clostridial botulinum serotype C1 HA-17 or a .beta.-trefoil domain
with enhanced binding activity derived from a Clostridial botulinum
serotype D HA-17. In another aspect of this embodiment, a
.beta.-trefoil domain with enhanced binding activity derived from a
Clostridial HA-17 comprises a modified .alpha.-fold motif of a
.beta.-trefoil domain of a Clostridial HA-17, a modified
.beta.-fold motif of a .beta.-trefoil domain of a Clostridial HA-17
or a modified .gamma.-fold motif of a .beta.-trefoil domain of a
Clostridial HA-17.
[0455] In another embodiment, a .beta.-trefoil domain derived from
a Clostridial botulinum serotype A HA-17 comprises a .beta.-trefoil
domain derived from a Clostridial botulinum serotype A HA-17 of SEQ
ID NO: 19. In another embodiment, a .beta.-trefoil domain derived
from a Clostridial botulinum serotype A HA-17 comprises amino acids
9-146 of SEQ ID NO: 19. In another aspect of this embodiment, a
.beta.-trefoil domain derived from a Clostridial botulinum serotype
A HA-17 comprises a .alpha.-fold motif of a .beta.-trefoil domain
of a Clostridial botulinum serotype A HA-17, a .beta.-fold motif of
a .beta.-trefoil domain of a Clostridial botulinum serotype A HA-17
or a .gamma.-fold motif of a .beta.-trefoil domain of a Clostridial
botulinum serotype A HA-17 of SEQ ID NO: 19. In another aspect of
this embodiment, a .beta.-trefoil domain derived from a Clostridial
botulinum serotype A HA-17 comprises amino acids 9-50, amino acids
55-91, or amino acids 95-146 of SEQ ID NO: 19.
[0456] In another embodiment, a .beta.-trefoil domain with enhanced
binding activity derived from a Clostridial botulinum serotype A
HA-17 comprises a .beta.-trefoil domain with enhanced binding
activity derived from a Clostridial botulinum serotype A HA-17 of
SEQ ID NO: 19. In another embodiment, a .beta.-trefoil domain with
enhanced binding activity derived from a Clostridial botulinum
serotype A HA-17 comprises amino acids 9-146 of SEQ ID NO: 19. In
another aspect of this embodiment, a .beta.-trefoil domain with
enhanced binding activity derived from a Clostridial botulinum
serotype A HA-17 comprises a modified .alpha.-fold motif of a
.beta.-trefoil domain of a Clostridial botulinum serotype A HA-17,
a modified .beta.-fold motif of a .beta.-trefoil domain of a
Clostridial botulinum serotype A HA-17 or a modified .gamma.-fold
motif of a 13-trefoil domain of a Clostridial botulinum serotype A
HA-17 of SEQ ID NO: 19. In another aspect of this embodiment, a
.beta.-trefoil domain with enhanced binding activity derived from a
Clostridial botulinum serotype A HA-17 comprises amino acids 9-50,
amino acids 55-91, or amino acids 95-146 of SEQ ID NO: 19.
[0457] In other aspects of this embodiment, a Clostridial botulinum
serotype A HA-17 comprising a .beta.-trefoil domain with enhanced
binding activity comprises a polypeptide having, e.g., at least 70%
amino acid identity with amino acids 9-50, amino acids 55-91, or
amino acids 95-146 of SEQ ID NO: 19, at least 75% amino acid
identity with amino acids 9-50, amino acids 55-91, or amino acids
95-146 of SEQ ID NO: 19, at least 80% amino acid identity with
amino acids 9-50, amino acids 55-91, or amino acids 95-146 of SEQ
ID NO: 19, at least 85% amino acid identity with amino acids 9-50,
amino acids 55-91, or amino acids 95-146 of SEQ ID NO: 19, at least
90% amino acid identity with amino acids 9-50, amino acids 55-91,
or amino acids 95-146 of SEQ ID NO: 19 or at least 95% amino acid
identity with amino acids 9-50, amino acids 55-91, or amino acids
95-146 of SEQ ID NO: 19. In yet other aspects of this embodiment, a
Clostridial botulinum serotype A HA-17 comprising a .beta.-trefoil
domain with enhanced binding activity comprises a polypeptide
having, e.g., at most 70% amino acid identity with amino acids
9-50, amino acids 55-91, or amino acids 95-146 of SEQ ID NO: 19, at
most 75% amino acid identity with amino acids 9-50, amino acids
55-91, or amino acids 95-146 of SEQ ID NO: 19, at most 80% amino
acid identity with amino acids 9-50, amino acids 55-91, or amino
acids 95-146 of SEQ ID NO: 19, at most 85% amino acid identity with
amino acids 9-50, amino acids 55-91, or amino acids 95-146 of SEQ
ID NO: 19, at most 90% amino acid identity with amino acids 9-50,
amino acids 55-91, or amino acids 95-146 of SEQ ID NO: 19 or at
most 95% amino acid identity with amino acids 9-50, amino acids
55-91, or amino acids 95-146 of SEQ ID NO: 19.
[0458] In other aspects of this embodiment, a Clostridial botulinum
serotype A HA-17 comprising a 11-trefoil domain with enhanced
binding activity comprises a polypeptide having, e.g., at most one,
two, three, four, five, six, seven, eight, nine, 10 or 20
non-contiguous amino acid substitutions relative to amino acids
9-50, amino acids 55-91, or amino acids 95-146 of SEQ ID NO: 19. In
other aspects of this embodiment, a Clostridial botulinum serotype
A HA-17 comprising a .beta.-trefoil domain with enhanced binding
activity comprises a polypeptide having, e.g., at least one, two,
three, four, five, six, seven, eight, nine, 10 or 20 non-contiguous
amino acid substitutions relative to amino acids 9-50, amino acids
55-91, or amino acids 95-146 of SEQ ID NO: 19. In yet other aspects
of this embodiment, a Clostridial botulinum serotype A HA-17
comprising a .beta.-trefoil domain with enhanced binding activity
comprises a polypeptide having, e.g., at most one, two, three,
four, five, six, seven, eight, nine, 10 or 20 non-contiguous amino
acid deletions relative to amino acids 9-50, amino acids 55-91, or
amino acids 95-146 of SEQ ID NO: 19. In other aspects of this
embodiment, a Clostridial botulinum serotype A HA-17 comprising a
.beta.-trefoil domain with enhanced binding activity comprises a
polypeptide having, e.g., at least one, two, three, four, five,
six, seven, eight, nine, 10 or 20 non-contiguous amino acid
deletions relative to amino acids 9-50, amino acids 55-91, or amino
acids 95-146 of SEQ ID NO: 19. In still other aspects of this
embodiment, a Clostridial botulinum serotype A HA-17 comprising a
.beta.-trefoil domain with enhanced binding activity comprises a
polypeptide having, e.g., at most one, two, three, four, five, six,
seven, eight, nine, 10 or 20 non-contiguous amino acid additions
relative to amino acids 9-50, amino acids 55-91, or amino acids
95-146 of SEQ ID NO: 19. In other aspects of this embodiment, a
Clostridial botulinum serotype A HA-17 comprising a .beta.-trefoil
domain with enhanced binding activity comprises a polypeptide
having, e.g., at least one, two, three, four, five, six, seven,
eight, nine, 10 or 20 non-contiguous amino acid additions relative
to amino acids 9-50, amino acids 55-91, or amino acids 95-146 of
SEQ ID NO: 19.
[0459] In other aspects of this embodiment, a Clostridial botulinum
serotype A HA-17 comprising a .beta.-trefoil domain with enhanced
binding activity comprises a polypeptide having, e.g., at most one,
two, three, four, five, six, seven, eight, nine, 10 or 20
contiguous amino acid substitutions relative to amino acids 9-50,
amino acids 55-91, or amino acids 95-146 of SEQ ID NO: 19. In other
aspects of this embodiment, a Clostridial botulinum serotype A
HA-17 comprising a .beta.-trefoil domain with enhanced binding
activity comprises a polypeptide having, e.g., at least one, two,
three, four, five, six, seven, eight, nine, 10 or 20 contiguous
amino acid substitutions relative to amino acids 9-50, amino acids
55-91, or amino acids 95-146 of SEQ ID NO: 19. In yet other aspects
of this embodiment, a Clostridial botulinum serotype A HA-17
comprising a .beta.-trefoil domain with enhanced binding activity
comprises a polypeptide having, e.g., at most one, two, three,
four, five, six, seven, eight, nine, 10 or 20 contiguous amino acid
deletions relative to amino acids 9-50, amino acids 55-91, or amino
acids 95-146 of SEQ ID NO: 19. In other aspects of this embodiment,
a Clostridial botulinum serotype A HA-17 comprising a
.beta.-trefoil domain with enhanced binding activity comprises a
polypeptide having, e.g., at least one, two, three, four, five,
six, seven, eight, nine, 10 or 20 contiguous amino acid deletions
relative to amino acids 9-50, amino acids 55-91, or amino acids
95-146 of SEQ ID NO: 19. In still other aspects of this embodiment,
a Clostridial botulinum serotype A HA-17 comprising a
.beta.-trefoil domain with enhanced binding activity comprises a
polypeptide having, e.g., at most one, two, three, four, five, six,
seven, eight, nine, 10 or 20 contiguous amino acid additions
relative to amino acids 9-50, amino acids 55-91, or amino acids
95-146 of SEQ ID NO: 19. In other aspects of this embodiment, a
Clostridial botulinum serotype A HA-17 comprising a .beta.-trefoil
domain with enhanced binding activity comprises a polypeptide
having, e.g., at least one, two, three, four, five, six, seven,
eight, nine, 10 or 20 contiguous amino acid additions relative to
amino acids 9-50, amino acids 55-91, or amino acids 95-146 of SEQ
ID NO: 19.
[0460] In another embodiment, a .beta.-trefoil domain derived from
a Clostridial botulinum serotype A HA-17 comprises a
.beta.4/.beta.5 hairpin turn of a .beta.-trefoil domain of a
Clostridial botulinum serotype A HA-17 or a .beta.8/.beta.9 hairpin
turn of a .beta.-trefoil domain of a Clostridial botulinum serotype
A HA-17 of SEQ ID NO: 19. In another aspect of this embodiment, a
.beta.-trefoil domain derived from a Clostridial botulinum serotype
A HA-17 comprises amino acids 51-54 or amino acids 92-94 of SEQ ID
NO: 19.
[0461] In another embodiment, a .beta.-trefoil domain with enhanced
binding activity derived from a Clostridial botulinum serotype A
HA-17 comprises a modified .beta.4/.beta.5 hairpin turn of a
.beta.-trefoil domain of a Clostridial botulinum serotype A HA-17
or a modified .beta.8/.beta.9 hairpin turn of a .beta.-trefoil
domain of a Clostridial botulinum serotype A HA-17 of SEQ ID NO:
19. In another aspect of this embodiment, a .beta.-trefoil domain
with enhanced binding activity derived from a Clostridial botulinum
serotype A HA-17 comprises a modification of amino acids 51-54 or
amino acids 92-94 of SEQ ID NO: 19.
[0462] In other aspects of this embodiment, a .beta.-trefoil domain
with enhanced binding activity derived from a Clostridial botulinum
serotype A HA-17 comprises a polypeptide having, e.g., at least 70%
amino acid identity with amino acids 51-54 or amino acids 92-94 of
SEQ ID NO: 19, at least 75% amino acid identity with amino acids
51-54 or amino acids 92-94 of SEQ ID NO: 19, at least 80% amino
acid identity with amino acids 51-54 or amino acids 92-94 of SEQ ID
NO: 19, at least 85% amino acid identity with amino acids 51-54 or
amino acids 92-94 of SEQ ID NO: 19, at least 90% amino acid
identity with amino acids 51-54 or amino acids 92-94 of SEQ ID NO:
19 or at least 95% amino acid identity with amino acids 51-54 or
amino acids 92-94 of SEQ ID NO: 19. In yet other aspects of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a Clostridial botulinum serotype A HA-17 comprises a
polypeptide having, e.g., at most 70% amino acid identity with
amino acids 51-54 or amino acids 92-94 of SEQ ID NO: 19, at most
75% amino acid identity with amino acids 51-54 or amino acids 92-94
of SEQ ID NO: 19, at most 80% amino acid identity with amino acids
51-54 or amino acids 92-94 of SEQ ID NO: 19, at most 85% amino acid
identity with amino acids 51-54 or amino acids 92-94 of SEQ ID NO:
19, at most 90% amino acid identity with amino acids 51-54 or amino
acids 92-94 of SEQ ID NO: 19 or at most 95% amino acid identity
with amino acids 51-54 or amino acids 92-94 of SEQ ID NO: 19.
[0463] In other aspects of this embodiment, a .beta.-trefoil domain
with enhanced binding activity derived from a Clostridial botulinum
serotype A HA-17 comprises a polypeptide having, e.g., at most one,
two, three or four non-contiguous amino acid substitutions relative
to amino acids 51-54 or amino acids 92-94 of SEQ ID NO: 19. In
other aspects of this embodiment, a .beta.-trefoil domain with
enhanced binding activity derived from a Clostridial botulinum
serotype A HA-17 comprises a polypeptide having, e.g., at least
one, two, three or four non-contiguous amino acid substitutions
relative to amino acids 51-54 or amino acids 92-94 of SEQ ID NO:
19. In other aspects of this embodiment, a non-contiguous amino
acid substitution of any amino acid from amino acids 51-54 or amino
acids 92-94 of SEQ ID NO: 19 can be replaced with glycine. In other
aspects of this embodiment, a non-contiguous amino acid
substitution of any hydrophobic amino acid from amino acids 51-54
or amino acids 92-94 of SEQ ID NO: 19 can be replaced with
phenylalanine. In yet other aspects of this embodiment, a
.beta.-trefoil domain with enhanced binding activity derived from a
Clostridial botulinum serotype A HA-17 comprises a polypeptide
having, e.g., at most one, two, three or four non-contiguous amino
acid deletions relative to amino acids 51-54 or amino acids 92-94
of SEQ ID NO: 19. In other aspects of this embodiment, a
.beta.-trefoil domain with enhanced binding activity derived from a
Clostridial botulinum serotype A HA-17 comprises a polypeptide
having, e.g., at least one, two, three or four non-contiguous amino
acid deletions relative to amino acids 51-54 or amino acids 92-94
of SEQ ID NO: 19. In still other aspects of this embodiment, a
.beta.-trefoil domain with enhanced binding activity derived from a
Clostridial botulinum serotype A HA-17 comprises a polypeptide
having, e.g., at most one, two, three or four non-contiguous amino
acid additions relative to amino acids 51-54 or amino acids 92-94
of SEQ ID NO: 19. In other aspects of this embodiment, a
.beta.-trefoil domain with enhanced binding activity derived from a
Clostridial botulinum serotype A HA-17 comprises a polypeptide
having, e.g., at least one, two, three or four non-contiguous amino
acid additions relative to amino acids 51-54 or amino acids 92-94
of SEQ ID NO: 19.
[0464] In other aspects of this embodiment, a .beta.-trefoil domain
with enhanced binding activity derived from a Clostridial botulinum
serotype A HA-17 comprises a polypeptide having, e.g., at most one,
two, three or four contiguous amino acid substitutions relative to
amino acids 51-54 or amino acids 92-94 of SEQ ID NO: 19. In other
aspects of this embodiment, a .beta.-trefoil domain with enhanced
binding activity derived from a Clostridial botulinum serotype A
HA-17 comprises a polypeptide having, e.g., at least one, two,
three or four contiguous amino acid substitutions relative to amino
acids 51-54 or amino acids 92-94 of SEQ ID NO: 19. In other aspects
of this embodiment, contiguous amino acid substitutions of amino
acids from amino acids 51-54 or amino acids 92-94 of SEQ ID NO: 19
can be replaced with glycine. In other aspects of this embodiment,
contiguous amino acid substitutions of hydrophobic amino acids from
amino acids 51-54 or amino acids 92-94 of SEQ ID NO: 19 can be
replaced with phenylalanine. In yet other aspects of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a Clostridial botulinum serotype A HA-17 comprises a
polypeptide having, e.g., at most one, two, three or four
contiguous amino acid deletions relative to amino acids 51-54 or
amino acids 92-94 of SEQ ID NO: 19. In other aspects of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a Clostridial botulinum serotype A HA-17 comprises a
polypeptide having, e.g., at least one, two, three or four
contiguous amino acid deletions relative to amino acids 51-54 or
amino acids 92-94 of SEQ ID NO: 19. In still other aspects of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a Clostridial botulinum serotype A HA-17 comprises a
polypeptide having, e.g., at most one, two, three or four
contiguous amino acid additions relative to amino acids 51-54 or
amino acids 92-94 of SEQ ID NO: 19. In other aspects of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a Clostridial botulinum serotype A HA-17 comprises a
polypeptide having, e.g., at least one, two, three or four
contiguous amino acid additions relative to amino acids 51-54 or
amino acids 92-94 of SEQ ID NO: 19.
[0465] In another embodiment, a .beta.-trefoil domain derived from
a Clostridial botulinum serotype B HA-17 comprises a .beta.-trefoil
domain derived from a Clostridial botulinum serotype B HA-17 of SEQ
ID NO: 20. In another embodiment, a .beta.-trefoil domain derived
from a Clostridial botulinum serotype B HA-17 comprises amino acids
9-146 of SEQ ID NO: 20. In another aspect of this embodiment, a
.beta.-trefoil domain derived from a Clostridial botulinum serotype
B HA-17 comprises a .alpha.-fold motif of a .beta.-trefoil domain
of a Clostridial botulinum serotype B HA-17, a .beta.-fold motif of
a .beta.-trefoil domain of a Clostridial botulinum serotype B HA-17
or a .gamma.-fold motif of a .beta.-trefoil domain of a Clostridial
botulinum serotype B HA-17 of SEQ ID NO: 20. In another aspect of
this embodiment, a .beta.-trefoil domain derived from a Clostridial
botulinum serotype B HA-17 comprises amino acids 9-50, amino acids
55-91, or amino acids 95-146 of SEQ ID NO: 20.
[0466] In another embodiment, a .beta.-trefoil domain with enhanced
binding activity derived from a Clostridial botulinum serotype B
HA-17 comprises a .beta.-trefoil domain with enhanced binding
activity derived from a Clostridial botulinum serotype B HA-17 of
SEQ ID NO: 20. In another embodiment, a .beta.-trefoil domain with
enhanced binding activity derived from a Clostridial botulinum
serotype B HA-17 comprises amino acids 9-146 of SEQ ID NO: 20. In
another aspect of this embodiment, a .beta.-trefoil domain with
enhanced binding activity derived from a Clostridial botulinum
serotype B HA-17 comprises a modified .alpha.-fold motif of a
.beta.-trefoil domain of a Clostridial botulinum serotype B HA-17,
a modified .beta.-fold motif of a .beta.-trefoil domain of a
Clostridial botulinum serotype B HA-17 or a modified .gamma.-fold
motif of a .beta.-trefoil domain of a Clostridial botulinum
serotype B HA-17 of SEQ ID NO: 20. In another aspect of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a Clostridial botulinum serotype B HA-17 comprises
amino acids 9-50, amino acids 55-91, or amino acids 95-146 of SEQ
ID NO: 20.
[0467] In other aspects of this embodiment, a Clostridial botulinum
serotype B HA-17 comprising a .beta.-trefoil domain with enhanced
binding activity comprises a polypeptide having, e.g., at least 70%
amino acid identity with amino acids 9-50, amino acids 55-91, or
amino acids 95-146 of SEQ ID NO: 20, at least 75% amino acid
identity with amino acids 9-50, amino acids 55-91, or amino acids
95-146 of SEQ ID NO: 20, at least 80% amino acid identity with
amino acids 9-50, amino acids 55-91, or amino acids 95-146 of SEQ
ID NO: 20, at least 85% amino acid identity with amino acids 9-50,
amino acids 55-91, or amino acids 95-146 of SEQ ID NO: 20, at least
90% amino acid identity with amino acids 9-50, amino acids 55-91,
or amino acids 95-146 of SEQ ID NO: 20 or at least 95% amino acid
identity with amino acids 9-50, amino acids 55-91, or amino acids
95-146 of SEQ ID NO: 20. In yet other aspects of this embodiment, a
Clostridial botulinum serotype B HA-17 comprising a .beta.-trefoil
domain with enhanced binding activity comprises a polypeptide
having, e.g., at most 70% amino acid identity with amino acids
9-50, amino acids 55-91, or amino acids 95-146 of SEQ ID NO: 20, at
most 75% amino acid identity with amino acids 9-50, amino acids
55-91, or amino acids 95-146 of SEQ ID NO: 20, at most 80% amino
acid identity with amino acids 9-50, amino acids 55-91, or amino
acids 95-146 of SEQ ID NO: 20, at most 85% amino acid identity with
amino acids 9-50, amino acids 55-91, or amino acids 95-146 of SEQ
ID NO: 20, at most 90% amino acid identity with amino acids 9-50,
amino acids 55-91, or amino acids 95-146 of SEQ ID NO: 20 or at
most 95% amino acid identity with amino acids 9-50, amino acids
55-91, or amino acids 95-146 of SEQ ID NO: 20.
[0468] In other aspects of this embodiment, a Clostridial botulinum
serotype B HA-17 comprising a .beta.-trefoil domain with enhanced
binding activity comprises a polypeptide having, e.g., at most one,
two, three, four, five, six, seven, eight, nine, 10 or 20
non-contiguous amino acid substitutions relative to amino acids
9-50, amino acids 55-91, or amino acids 95-146 of SEQ ID NO: 20. In
other aspects of this embodiment, a Clostridial botulinum serotype
B HA-17 comprising a .beta.-trefoil domain with enhanced binding
activity comprises a polypeptide having, e.g., at least one, two,
three, four, five, six, seven, eight, nine, 10 or 20 non-contiguous
amino acid substitutions relative to amino acids 9-50, amino acids
55-91, or amino acids 95-146 of SEQ ID NO: 20. In yet other aspects
of this embodiment, a Clostridial botulinum serotype B HA-17
comprising a .beta.-trefoil domain with enhanced binding activity
comprises a polypeptide having, e.g., at most one, two, three,
four, five, six, seven, eight, nine, 10 or 20 non-contiguous amino
acid deletions relative to amino acids 9-50, amino acids 55-91, or
amino acids 95-146 of SEQ ID NO: 20. In other aspects of this
embodiment, a Clostridial botulinum serotype B HA-17 comprising a
.beta.-trefoil domain with enhanced binding activity comprises a
polypeptide having, e.g., at least one, two, three, four, five,
six, seven, eight, nine, 10 or 20 non-contiguous amino acid
deletions relative to amino acids 9-50, amino acids 55-91, or amino
acids 95-146 of SEQ ID NO: 20. In still other aspects of this
embodiment, a Clostridial botulinum serotype B HA-17 comprising a
.beta.-trefoil domain with enhanced binding activity comprises a
polypeptide having, e.g., at most one, two, three, four, five, six,
seven, eight, nine, 10 or 20 non-contiguous amino acid additions
relative to amino acids 9-50, amino acids 55-91, or amino acids
95-146 of SEQ ID NO: 20. In other aspects of this embodiment, a
Clostridial botulinum serotype B HA-17 comprising a .beta.-trefoil
domain with enhanced binding activity comprises a polypeptide
having, e.g., at least one, two, three, four, five, six, seven,
eight, nine, 10 or 20 non-contiguous amino acid additions relative
to amino acids 9-50, amino acids 55-91, or amino acids 95-146 of
SEQ ID NO: 20.
[0469] In other aspects of this embodiment, a Clostridial botulinum
serotype B HA-17 comprising a .beta.-trefoil domain with enhanced
binding activity comprises a polypeptide having, e.g., at most one,
two, three, four, five, six, seven, eight, nine, 10 or 20
contiguous amino acid substitutions relative to amino acids 9-50,
amino acids 55-91, or amino acids 95-146 of SEQ ID NO: 20. In other
aspects of this embodiment, a Clostridial botulinum serotype B
HA-17 comprising a .beta.-trefoil domain with enhanced binding
activity comprises a polypeptide having, e.g., at least one, two,
three, four, five, six, seven, eight, nine, 10 or 20 contiguous
amino acid substitutions relative to amino acids 9-50, amino acids
55-91, or amino acids 95-146 of SEQ ID NO: 20. In yet other aspects
of this embodiment, a Clostridial botulinum serotype B HA-17
comprising a .beta.-trefoil domain with enhanced binding activity
comprises a polypeptide having, e.g., at most one, two, three,
four, five, six, seven, eight, nine, 10 or 20 contiguous amino acid
deletions relative to amino acids 9-50, amino acids 55-91, or amino
acids 95-146 of SEQ ID NO: 20. In other aspects of this embodiment,
a Clostridial botulinum serotype B HA-17 comprising a
.beta.-trefoil domain with enhanced binding activity comprises a
polypeptide having, e.g., at least one, two, three, four, five,
six, seven, eight, nine, 10 or 20 contiguous amino acid deletions
relative to amino acids 9-50, amino acids 55-91, or amino acids
95-146 of SEQ ID NO: 20. In still other aspects of this embodiment,
a Clostridial botulinum serotype B HA-17 comprising a
.beta.-trefoil domain with enhanced binding activity comprises a
polypeptide having, e.g., at most one, two, three, four, five, six,
seven, eight, nine, 10 or 20 contiguous amino acid additions
relative to amino acids 9-50, amino acids 55-91, or amino acids
95-146 of SEQ ID NO: 20. In other aspects of this embodiment, a
Clostridial botulinum serotype B HA-17 comprising a .beta.-trefoil
domain with enhanced binding activity comprises a polypeptide
having, e.g., at least one, two, three, four, five, six, seven,
eight, nine, 10 or 20 contiguous amino acid additions relative to
amino acids 9-50, amino acids 55-91, or amino acids 95-146 of SEQ
ID NO: 20.
[0470] In another embodiment, a .beta.-trefoil domain derived from
a Clostridial botulinum serotype B HA-17 comprises a
.beta.4/.beta.5 hairpin turn of a .beta.-trefoil domain of a
Clostridial botulinum serotype B HA-17 or a .beta.8/.beta.9 hairpin
turn of a .beta.-trefoil domain of a Clostridial botulinum serotype
B HA-17 of SEQ ID NO: 20. In another aspect of this embodiment, a
.beta.-trefoil domain derived from a Clostridial botulinum serotype
B HA-17 comprises amino acids 51-54 or amino acids 92-94 of SEQ ID
NO: 20.
[0471] In another embodiment, a .beta.-trefoil domain with enhanced
binding activity derived from a Clostridial botulinum serotype B
HA-17 comprises a modified .beta.4/.beta.5 hairpin turn of a
.beta.-trefoil domain of a Clostridial botulinum serotype B HA-17
or a modified .beta.8/.beta.9 hairpin turn of a .beta.-trefoil
domain of a Clostridial botulinum serotype B HA-17 of SEQ ID NO:
20. In another aspect of this embodiment, a .beta.-trefoil domain
with enhanced binding activity derived from a Clostridial botulinum
serotype B HA-17 comprises a modification of amino acids 51-54 or
amino acids 92-94 of SEQ ID NO: 20.
[0472] In other aspects of this embodiment, a .beta.-trefoil domain
with enhanced binding activity derived from a Clostridial botulinum
serotype B HA-17 comprises a polypeptide having, e.g., at least 70%
amino acid identity with amino acids 51-54 or amino acids 92-94 of
SEQ ID NO: 20, at least 75% amino acid identity with amino acids
51-54 or amino acids 92-94 of SEQ ID NO: 20, at least 80% amino
acid identity with amino acids 51-54 or amino acids 92-94 of SEQ ID
NO: 20, at least 85% amino acid identity with amino acids 51-54 or
amino acids 92-94 of SEQ ID NO: 20, at least 90% amino acid
identity with amino acids 51-54 or amino acids 92-94 of SEQ ID NO:
20 or at least 95% amino acid identity with amino acids 51-54 or
amino acids 92-94 of SEQ ID NO: 20. In yet other aspects of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a Clostridial botulinum serotype B HA-17 comprises a
polypeptide having, e.g., at most 70% amino acid identity with
amino acids 51-54 or amino acids 92-94 of SEQ ID NO: 20, at most
75% amino acid identity with amino acids 51-54 or amino acids 92-94
of SEQ ID NO: 20, at most 80% amino acid identity with amino acids
51-54 or amino acids 92-94 of SEQ ID NO: 20, at most 85% amino acid
identity with amino acids 51-54 or amino acids 92-94 of SEQ ID NO:
20, at most 90% amino acid identity with amino acids 51-54 or amino
acids 92-94 of SEQ ID NO: 20 or at most 95% amino acid identity
with amino acids 51-54 or amino acids 92-94 of SEQ ID NO: 20.
[0473] In other aspects of this embodiment, a .beta.-trefoil domain
with enhanced binding activity derived from a Clostridial botulinum
serotype B HA-17 comprises a polypeptide having, e.g., at most one,
two, three or four non-contiguous amino acid substitutions relative
to amino acids 51-54 or amino acids 92-94 of SEQ ID NO: 20. In
other aspects of this embodiment, a .beta.-trefoil domain with
enhanced binding activity derived from a Clostridial botulinum
serotype B HA-17 comprises a polypeptide having, e.g., at least
one, two, three or four non-contiguous amino acid substitutions
relative to amino acids 51-54 or amino acids 92-94 of SEQ ID NO:
20. In other aspects of this embodiment, a non-contiguous amino
acid substitution of any amino acid from amino acids 51-54 or amino
acids 92-94 of SEQ ID NO: 20 can be replaced with glycine. In other
aspects of this embodiment, a non-contiguous amino acid
substitution of any hydrophobic amino acid from amino acids 51-54
or amino acids 92-94 of SEQ ID NO: 20 can be replaced with
phenylalanine. In yet other aspects of this embodiment, a
.beta.-trefoil domain with enhanced binding activity derived from a
Clostridial botulinum serotype B HA-17 comprises a polypeptide
having, e.g., at most one, two, three or four non-contiguous amino
acid deletions relative to amino acids 51-54 or amino acids 92-94
of SEQ ID NO: 20. In other aspects of this embodiment, a
.beta.-trefoil domain with enhanced binding activity derived from a
Clostridial botulinum serotype B HA-17 comprises a polypeptide
having, e.g., at least one, two, three or four non-contiguous amino
acid deletions relative to amino acids 51-54 or amino acids 92-94
of SEQ ID NO: 20. In still other aspects of this embodiment, a
.beta.-trefoil domain with enhanced binding activity derived from a
Clostridial botulinum serotype B HA-17 comprises a polypeptide
having, e.g., at most one, two, three or four non-contiguous amino
acid additions relative to amino acids 51-54 or amino acids 92-94
of SEQ ID NO: 20. In other aspects of this embodiment, a
.beta.-trefoil domain with enhanced binding activity derived from a
Clostridial botulinum serotype B HA-17 comprises a polypeptide
having, e.g., at least one, two, three or four non-contiguous amino
acid additions relative to amino acids 51-54 or amino acids 92-94
of SEQ ID NO: 20.
[0474] In other aspects of this embodiment, a .beta.-trefoil domain
with enhanced binding activity derived from a Clostridial botulinum
serotype B HA-17 comprises a polypeptide having, e.g., at most one,
two, three or four contiguous amino acid substitutions relative to
amino acids 51-54 or amino acids 92-94 of SEQ ID NO: 20. In other
aspects of this embodiment, a .beta.-trefoil domain with enhanced
binding activity derived from a Clostridial botulinum serotype B
HA-17 comprises a polypeptide having, e.g., at least one, two,
three or four contiguous amino acid substitutions relative to amino
acids 51-54 or amino acids 92-94 of SEQ ID NO: 20. In other aspects
of this embodiment, contiguous amino acid substitutions of amino
acids from amino acids 51-54 or amino acids 92-94 of SEQ ID NO: 20
can be replaced with glycine. In other aspects of this embodiment,
contiguous amino acid substitutions of hydrophobic amino acids from
amino acids 51-54 or amino acids 92-94 of SEQ ID NO: 20 can be
replaced with phenylalanine. In yet other aspects of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a Clostridial botulinum serotype B HA-17 comprises a
polypeptide having, e.g., at most one, two, three or four
contiguous amino acid deletions relative to amino acids 51-54 or
amino acids 92-94 of SEQ ID NO: 20. In other aspects of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a Clostridial botulinum serotype B HA-17 comprises a
polypeptide having, e.g., at least one, two, three or four
contiguous amino acid deletions relative to amino acids 51-54 or
amino acids 92-94 of SEQ ID NO: 20. In still other aspects of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a Clostridial botulinum serotype B HA-17 comprises a
polypeptide having, e.g., at most one, two, three or four
contiguous amino acid additions relative to amino acids 51-54 or
amino acids 92-94 of SEQ ID NO: 20. In other aspects of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a Clostridial botulinum serotype B HA-17 comprises a
polypeptide having, e.g., at least one, two, three or four
contiguous amino acid additions relative to amino acids 51-54 or
amino acids 92-94 of SEQ ID NO: 20.
[0475] In another embodiment, a .beta.-trefoil domain derived from
a Clostridial botulinum serotype C1 HA-17 comprises a
.beta.-trefoil domain derived from a Clostridial botulinum serotype
C1 HA-17 of SEQ ID NO: 21. In another embodiment, a .beta.-trefoil
domain derived from a Clostridial botulinum serotype C1 HA-17
comprises amino acids 9-146 of SEQ ID NO: 21. In another aspect of
this embodiment, a .beta.-trefoil domain derived from a Clostridial
botulinum serotype C1 HA-17 comprises a .alpha.-fold motif of a
.beta.-trefoil domain of a Clostridial botulinum serotype C1 HA-17,
a .beta.-fold motif of a .beta.-trefoil domain of a Clostridial
botulinum serotype C1 HA-17 or a .gamma.-fold motif of a
.beta.-trefoil domain of a Clostridial botulinum serotype C1 HA-17
of SEQ ID NO: 21. In another aspect of this embodiment, a
.beta.-trefoil domain derived from a Clostridial botulinum serotype
C1 HA-17 comprises amino acids 9-50, amino acids 55-91, or amino
acids 95-146 of SEQ ID NO: 21.
[0476] In another embodiment, a .beta.-trefoil domain with enhanced
binding activity derived from a Clostridial botulinum serotype C1
HA-17 comprises a .beta.-trefoil domain with enhanced binding
activity derived from a Clostridial botulinum serotype C1 HA-17 of
SEQ ID NO: 21. In another embodiment, a .beta.-trefoil domain with
enhanced binding activity derived from a Clostridial botulinum
serotype C1 HA-17 comprises amino acids 9-146 of SEQ ID NO: 21. In
another aspect of this embodiment, a .beta.-trefoil domain with
enhanced binding activity derived from a Clostridial botulinum
serotype C1 HA-17 comprises a modified .alpha.-fold motif of a
.beta.-trefoil domain of a Clostridial botulinum serotype C1 HA-17,
a modified .beta.-fold motif of a .beta.-trefoil domain of a
Clostridial botulinum serotype C1 HA-17 or a modified .gamma.-fold
motif of a .beta.-trefoil domain of a Clostridial botulinum
serotype C1 HA-17 of SEQ ID NO: 21. In another aspect of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a Clostridial botulinum serotype C1 HA-17 comprises
amino acids 9-50, amino acids 55-91, or amino acids 95-146 of SEQ
ID NO: 21.
[0477] In other aspects of this embodiment, a Clostridial botulinum
serotype C1 HA-17 comprising a .beta.-trefoil domain with enhanced
binding activity comprises a polypeptide having, e.g., at least 70%
amino acid identity with amino acids 9-50, amino acids 55-91, or
amino acids 95-146 of SEQ ID NO: 21, at least 75% amino acid
identity with amino acids 9-50, amino acids 55-91, or amino acids
95-146 of SEQ ID NO: 21, at least 80% amino acid identity with
amino acids 9-50, amino acids 55-91, or amino acids 95-146 of SEQ
ID NO: 21, at least 85% amino acid identity with amino acids 9-50,
amino acids 55-91, or amino acids 95-146 of SEQ ID NO: 21, at least
90% amino acid identity with amino acids 9-50, amino acids 55-91,
or amino acids 95-146 of SEQ ID NO: 21 or at least 95% amino acid
identity with amino acids 9-50, amino acids 55-91, or amino acids
95-146 of SEQ ID NO: 21. In yet other aspects of this embodiment, a
Clostridial botulinum serotype C1 HA-17 comprising a .beta.-trefoil
domain with enhanced binding activity comprises a polypeptide
having, e.g., at most 70% amino acid identity with amino acids
9-50, amino acids 55-91, or amino acids 95-146 of SEQ ID NO: 21, at
most 75% amino acid identity with amino acids 9-50, amino acids
55-91, or amino acids 95-146 of SEQ ID NO: 21, at most 80% amino
acid identity with amino acids 9-50, amino acids 55-91, or amino
acids 95-146 of SEQ ID NO: 21, at most 85% amino acid identity with
amino acids 9-50, amino acids 55-91, or amino acids 95-146 of SEQ
ID NO: 21, at most 90% amino acid identity with amino acids 9-50,
amino acids 55-91, or amino acids 95-146 of SEQ ID NO: 21 or at
most 95% amino acid identity with amino acids 9-50, amino acids
55-91, or amino acids 95-146 of SEQ ID NO: 21.
[0478] In other aspects of this embodiment, a Clostridial botulinum
serotype C1 HA-17 comprising a .beta.-trefoil domain with enhanced
binding activity comprises a polypeptide having, e.g., at most one,
two, three, four, five, six, seven, eight, nine, 10 or 20
non-contiguous amino acid substitutions relative to amino acids
9-50, amino acids 55-91, or amino acids 95-146 of SEQ ID NO: 21. In
other aspects of this embodiment, a Clostridial botulinum serotype
C1 HA-17 comprising a .beta.-trefoil domain with enhanced binding
activity comprises a polypeptide having, e.g., at least one, two,
three, four, five, six, seven, eight, nine, 10 or 20 non-contiguous
amino acid substitutions relative to amino acids 9-50, amino acids
55-91, or amino acids 95-146 of SEQ ID NO: 21. In yet other aspects
of this embodiment, a Clostridial botulinum serotype C1 HA-17
comprising a .beta.-trefoil domain with enhanced binding activity
comprises a polypeptide having, e.g., at most one, two, three,
four, five, six, seven, eight, nine, 10 or 20 non-contiguous amino
acid deletions relative to amino acids 9-50, amino acids 55-91, or
amino acids 95-146 of SEQ ID NO: 21. In other aspects of this
embodiment, a Clostridial botulinum serotype C1 HA-17 comprising a
.beta.-trefoil domain with enhanced binding activity comprises a
polypeptide having, e.g., at least one, two, three, four, five,
six, seven, eight, nine, 10 or 20 non-contiguous amino acid
deletions relative to amino acids 9-50, amino acids 55-91, or amino
acids 95-146 of SEQ ID NO: 21. In still other aspects of this
embodiment, a Clostridial botulinum serotype C1 HA-17 comprising a
.beta.-trefoil domain with enhanced binding activity comprises a
polypeptide having, e.g., at most one, two, three, four, five, six,
seven, eight, nine, 10 or 20 non-contiguous amino acid additions
relative to amino acids 9-50, amino acids 55-91, or amino acids
95-146 of SEQ ID NO: 21. In other aspects of this embodiment, a
Clostridial botulinum serotype C1 HA-17 comprising a .beta.-trefoil
domain with enhanced binding activity comprises a polypeptide
having, e.g., at least one, two, three, four, five, six, seven,
eight, nine, 10 or 20 non-contiguous amino acid additions relative
to amino acids 9-50, amino acids 55-91, or amino acids 95-146 of
SEQ ID NO: 21.
[0479] In other aspects of this embodiment, a Clostridial botulinum
serotype C1 HA-17 comprising a .beta.-trefoil domain with enhanced
binding activity comprises a polypeptide having, e.g., at most one,
two, three, four, five, six, seven, eight, nine, 10 or 20
contiguous amino acid substitutions relative to amino acids 9-50,
amino acids 55-91, or amino acids 95-146 of SEQ ID NO: 21. In other
aspects of this embodiment, a Clostridial botulinum serotype C1
HA-17 comprising a .beta.-trefoil domain with enhanced binding
activity comprises a polypeptide having, e.g., at least one, two,
three, four, five, six, seven, eight, nine, 10 or 20 contiguous
amino acid substitutions relative to amino acids 9-50, amino acids
55-91, or amino acids 95-146 of SEQ ID NO: 21. In yet other aspects
of this embodiment, a Clostridial botulinum serotype C1 HA-17
comprising a .beta.-trefoil domain with enhanced binding activity
comprises a polypeptide having, e.g., at most one, two, three,
four, five, six, seven, eight, nine, 10 or 20 contiguous amino acid
deletions relative to amino acids 9-50, amino acids 55-91, or amino
acids 95-146 of SEQ ID NO: 21. In other aspects of this embodiment,
a Clostridial botulinum serotype C1 HA-17 comprising a
.beta.-trefoil domain with enhanced binding activity comprises a
polypeptide having, e.g., at least one, two, three, four, five,
six, seven, eight, nine, 10 or 20 contiguous amino acid deletions
relative to amino acids 9-50, amino acids 55-91, or amino acids
95-146 of SEQ ID NO: 21. In still other aspects of this embodiment,
a Clostridial botulinum serotype C1 HA-17 comprising a
.beta.-trefoil domain with enhanced binding activity comprises a
polypeptide having, e.g., at most one, two, three, four, five, six,
seven, eight, nine, 10 or 20 contiguous amino acid additions
relative to amino acids 9-50, amino acids 55-91, or amino acids
95-146 of SEQ ID NO: 21. In other aspects of this embodiment, a
Clostridial botulinum serotype C1 HA-17 comprising a .beta.-trefoil
domain with enhanced binding activity comprises a polypeptide
having, e.g., at least one, two, three, four, five, six, seven,
eight, nine, 10 or 20 contiguous amino acid additions relative to
amino acids 9-50, amino acids 55-91, or amino acids 95-146 of SEQ
ID NO: 21.
[0480] In another embodiment, a .beta.-trefoil domain derived from
a Clostridial botulinum serotype C1 HA-17 comprises a
8.beta.4/.beta.5 hairpin turn of a .beta.-trefoil domain of a
Clostridial botulinum serotype C1 HA-17 or a 8.beta.8/.beta.9
hairpin turn of a .beta.-trefoil domain of a Clostridial botulinum
serotype C1 HA-17 of SEQ ID NO: 21. In another aspect of this
embodiment, a .beta.-trefoil domain derived from a Clostridial
botulinum serotype C1 HA-17 comprises amino acids 51-54 or amino
acids 92-94 of SEQ ID NO: 21.
[0481] In another embodiment, a .beta.-trefoil domain with enhanced
binding activity derived from a Clostridial botulinum serotype C1
HA-17 comprises a modified 8.beta.4/.beta.5 hairpin turn of a
.beta.-trefoil domain of a Clostridial botulinum serotype C1 HA-17
or a modified .beta.8/.beta.9 hairpin turn of a .beta.-trefoil
domain of a Clostridial botulinum serotype C1 HA-17 of SEQ ID NO:
21. In another aspect of this embodiment, a 11-trefoil domain with
enhanced binding activity derived from a Clostridial botulinum
serotype C1 HA-17 comprises a modification of amino acids 51-54 or
amino acids 92-94 of SEQ ID NO: 21.
[0482] In other aspects of this embodiment, a .beta.-trefoil domain
with enhanced binding activity derived from a Clostridial botulinum
serotype C1 HA-17 comprises a polypeptide having, e.g., at least
70% amino acid identity with amino acids 51-54 or amino acids 92-94
of SEQ ID NO: 21, at least 75% amino acid identity with amino acids
51-54 or amino acids 92-94 of SEQ ID NO: 21, at least 80% amino
acid identity with amino acids 51-54 or amino acids 92-94 of SEQ ID
NO: 21, at least 85% amino acid identity with amino acids 51-54 or
amino acids 92-94 of SEQ ID NO: 21, at least 90% amino acid
identity with amino acids 51-54 or amino acids 92-94 of SEQ ID NO:
21 or at least 95% amino acid identity with amino acids 51-54 or
amino acids 92-94 of SEQ ID NO: 21. In yet other aspects of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a Clostridial botulinum serotype C1 HA-17 comprises a
polypeptide having, e.g., at most 70% amino acid identity with
amino acids 51-54 or amino acids 92-94 of SEQ ID NO: 21, at most
75% amino acid identity with amino acids 51-54 or amino acids 92-94
of SEQ ID NO: 21, at most 80% amino acid identity with amino acids
51-54 or amino acids 92-94 of SEQ ID NO: 21, at most 85% amino acid
identity with amino acids 51-54 or amino acids 92-94 of SEQ ID NO:
21, at most 90% amino acid identity with amino acids 51-54 or amino
acids 92-94 of SEQ ID NO: 21 or at most 95% amino acid identity
with amino acids 51-54 or amino acids 92-94 of SEQ ID NO: 21.
[0483] In other aspects of this embodiment, a .beta.-trefoil domain
with enhanced binding activity derived from a Clostridial botulinum
serotype C1 HA-17 comprises a polypeptide having, e.g., at most
one, two, three or four non-contiguous amino acid substitutions
relative to amino acids 51-54 or amino acids 92-94 of SEQ ID NO:
21. In other aspects of this embodiment, a .beta.-trefoil domain
with enhanced binding activity derived from a Clostridial botulinum
serotype C1 HA-17 comprises a polypeptide having, e.g., at least
one, two, three or four non-contiguous amino acid substitutions
relative to amino acids 51-54 or amino acids 92-94 of SEQ ID NO:
21. In other aspects of this embodiment, a non-contiguous amino
acid substitution of any amino acid from amino acids 51-54 or amino
acids 92-94 of SEQ ID NO: 21 can be replaced with glycine. In other
aspects of this embodiment, a non-contiguous amino acid
substitution of any hydrophobic amino acid from amino acids 51-54
or amino acids 92-94 of SEQ ID NO: 21 can be replaced with
phenylalanine. In yet other aspects of this embodiment, a
.beta.-trefoil domain with enhanced binding activity derived from a
Clostridial botulinum serotype C1 HA-17 comprises a polypeptide
having, e.g., at most one, two, three or four non-contiguous amino
acid deletions relative to amino acids 51-54 or amino acids 92-94
of SEQ ID NO: 21. In other aspects of this embodiment, a
.beta.-trefoil domain with enhanced binding activity derived from a
Clostridial botulinum serotype C1 HA-17 comprises a polypeptide
having, e.g., at least one, two, three or four non-contiguous amino
acid deletions relative to amino acids 51-54 or amino acids 92-94
of SEQ ID NO: 21. In still other aspects of this embodiment, a
.beta.-trefoil domain with enhanced binding activity derived from a
Clostridial botulinum serotype C1 HA-17 comprises a polypeptide
having, e.g., at most one, two, three or four non-contiguous amino
acid additions relative to amino acids 51-54 or amino acids 92-94
of SEQ ID NO: 21. In other aspects of this embodiment, a
.beta.-trefoil domain with enhanced binding activity derived from a
Clostridial botulinum serotype C1 HA-17 comprises a polypeptide
having, e.g., at least one, two, three or four non-contiguous amino
acid additions relative to amino acids 51-54 or amino acids 92-94
of SEQ ID NO: 21.
[0484] In other aspects of this embodiment, a .beta.-trefoil domain
with enhanced binding activity derived from a Clostridial botulinum
serotype C1 HA-17 comprises a polypeptide having, e.g., at most
one, two, three or four contiguous amino acid substitutions
relative to amino acids 51-54 or amino acids 92-94 of SEQ ID NO:
21. In other aspects of this embodiment, a .beta.-trefoil domain
with enhanced binding activity derived from a Clostridial botulinum
serotype C1 HA-17 comprises a polypeptide having, e.g., at least
one, two, three or four contiguous amino acid substitutions
relative to amino acids 51-54 or amino acids 92-94 of SEQ ID NO:
21. In other aspects of this embodiment, contiguous amino acid
substitutions of amino acids from amino acids 51-54 or amino acids
92-94 of SEQ ID NO: 21 can be replaced with glycine. In other
aspects of this embodiment, contiguous amino acid substitutions of
hydrophobic amino acids from amino acids 51-54 or amino acids 92-94
of SEQ ID NO: 21 can be replaced with phenylalanine. In yet other
aspects of this embodiment, a .beta.-trefoil domain with enhanced
binding activity derived from a Clostridial botulinum serotype C1
HA-17 comprises a polypeptide having, e.g., at most one, two, three
or four contiguous amino acid deletions relative to amino acids
51-54 or amino acids 92-94 of SEQ ID NO: 21. In other aspects of
this embodiment, a .beta.-trefoil domain with enhanced binding
activity derived from a Clostridial botulinum serotype C1 HA-17
comprises a polypeptide having, e.g., at least one, two, three or
four contiguous amino acid deletions relative to amino acids 51-54
or amino acids 92-94 of SEQ ID NO: 21. In still other aspects of
this embodiment, a .beta.-trefoil domain with enhanced binding
activity derived from a Clostridial botulinum serotype C1 HA-17
comprises a polypeptide having, e.g., at most one, two, three or
four contiguous amino acid additions relative to amino acids 51-54
or amino acids 92-94 of SEQ ID NO: 21. In other aspects of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a Clostridial botulinum serotype C1 HA-17 comprises a
polypeptide having, e.g., at least one, two, three or four
contiguous amino acid additions relative to amino acids 51-54 or
amino acids 92-94 of SEQ ID NO: 21.
[0485] In another embodiment, a .beta.-trefoil domain derived from
a Clostridial botulinum serotype D HA-17 comprises a .beta.-trefoil
domain derived from a Clostridial botulinum serotype D HA-17 of SEQ
ID NO: 22. In another embodiment, a .beta.-trefoil domain derived
from a Clostridial botulinum serotype D HA-17 comprises amino acids
9-146 of SEQ ID NO: 22. In another aspect of this embodiment, a
.beta.-trefoil domain derived from a Clostridial botulinum serotype
D HA-17 comprises a .alpha.-fold motif of a .beta.-trefoil domain
of a Clostridial botulinum serotype D HA-17, a .beta.-fold motif of
a .beta.-trefoil domain of a Clostridial botulinum serotype D HA-17
or a .gamma.-fold motif of a .beta.-trefoil domain of a Clostridial
botulinum serotype D HA-17 of SEQ ID NO: 22. In another aspect of
this embodiment, a .beta.-trefoil domain derived from a Clostridial
botulinum serotype D HA-17 comprises amino acids 9-50, amino acids
55-91, or amino acids 95-146 of SEQ ID NO: 22.
[0486] In another embodiment, a .beta.-trefoil domain with enhanced
binding activity derived from a Clostridial botulinum serotype D
HA-17 comprises a .beta.-trefoil domain with enhanced binding
activity derived from a Clostridial botulinum serotype D HA-17 of
SEQ ID NO: 22. In another embodiment, a .beta.-trefoil domain with
enhanced binding activity derived from a Clostridial botulinum
serotype D HA-17 comprises amino acids 9-146 of SEQ ID NO: 22. In
another aspect of this embodiment, a .beta.-trefoil domain with
enhanced binding activity derived from a Clostridial botulinum
serotype D HA-17 comprises a modified .alpha.-fold motif of a
.beta.-trefoil domain of a Clostridial botulinum serotype D HA-17,
a modified .beta.-fold motif of a .beta.-trefoil domain of a
Clostridial botulinum serotype D HA-17 or a modified .gamma.-fold
motif of a 13-trefoil domain of a Clostridial botulinum serotype D
HA-17 of SEQ ID NO: 22. In another aspect of this embodiment, a
.beta.-trefoil domain with enhanced binding activity derived from a
Clostridial botulinum serotype D HA-17 comprises amino acids 9-50,
amino acids 55-91, or amino acids 95-146 of SEQ ID NO: 22.
[0487] In other aspects of this embodiment, a Clostridial botulinum
serotype D HA-17 comprising a .beta.-trefoil domain with enhanced
binding activity comprises a polypeptide having, e.g., at least 70%
amino acid identity with amino acids 9-50, amino acids 55-91, or
amino acids 95-146 of SEQ ID NO: 22, at least 75% amino acid
identity with amino acids 9-50, amino acids 55-91, or amino acids
95-146 of SEQ ID NO: 22, at least 80% amino acid identity with
amino acids 9-50, amino acids 55-91, or amino acids 95-146 of SEQ
ID NO: 22, at least 85% amino acid identity with amino acids 9-50,
amino acids 55-91, or amino acids 95-146 of SEQ ID NO: 22, at least
90% amino acid identity with amino acids 9-50, amino acids 55-91,
or amino acids 95-146 of SEQ ID NO: 22 or at least 95% amino acid
identity with amino acids 9-50, amino acids 55-91, or amino acids
95-146 of SEQ ID NO: 22. In yet other aspects of this embodiment, a
Clostridial botulinum serotype D HA-17 comprising a .beta.-trefoil
domain with enhanced binding activity comprises a polypeptide
having, e.g., at most 70% amino acid identity with amino acids
9-50, amino acids 55-91, or amino acids 95-146 of SEQ ID NO: 22, at
most 75% amino acid identity with amino acids 9-50, amino acids
55-91, or amino acids 95-146 of SEQ ID NO: 22, at most 80% amino
acid identity with amino acids 9-50, amino acids 55-91, or amino
acids 95-146 of SEQ ID NO: 22, at most 85% amino acid identity with
amino acids 9-50, amino acids 55-91, or amino acids 95-146 of SEQ
ID NO: 22, at most 90% amino acid identity with amino acids 9-50,
amino acids 55-91, or amino acids 95-146 of SEQ ID NO: 22 or at
most 95% amino acid identity with amino acids 9-50, amino acids
55-91, or amino acids 95-146 of SEQ ID NO: 22.
[0488] In other aspects of this embodiment, a Clostridial botulinum
serotype D HA-17 comprising a .beta.-trefoil domain with enhanced
binding activity comprises a polypeptide having, e.g., at most one,
two, three, four, five, six, seven, eight, nine, 10 or 20
non-contiguous amino acid substitutions relative to amino acids
9-50, amino acids 55-91, or amino acids 95-146 of SEQ ID NO: 22. In
other aspects of this embodiment, a Clostridial botulinum serotype
D HA-17 comprising a .beta.-trefoil domain with enhanced binding
activity comprises a polypeptide having, e.g., at least one, two,
three, four, five, six, seven, eight, nine, 10 or 20 non-contiguous
amino acid substitutions relative to amino acids 9-50, amino acids
55-91, or amino acids 95-146 of SEQ ID NO: 22. In yet other aspects
of this embodiment, a Clostridial botulinum serotype D HA-17
comprising a .beta.-trefoil domain with enhanced binding activity
comprises a polypeptide having, e.g., at most one, two, three,
four, five, six, seven, eight, nine, 10 or 20 non-contiguous amino
acid deletions relative to amino acids 9-50, amino acids 55-91, or
amino acids 95-146 of SEQ ID NO: 22. In other aspects of this
embodiment, a Clostridial botulinum serotype D HA-17 comprising a
.beta.-trefoil domain with enhanced binding activity comprises a
polypeptide having, e.g., at least one, two, three, four, five,
six, seven, eight, nine, 10 or 20 non-contiguous amino acid
deletions relative to amino acids 9-50, amino acids 55-91, or amino
acids 95-146 of SEQ ID NO: 22. In still other aspects of this
embodiment, a Clostridial botulinum serotype D HA-17 comprising a
.beta.-trefoil domain with enhanced binding activity comprises a
polypeptide having, e.g., at most one, two, three, four, five, six,
seven, eight, nine, 10 or 20 non-contiguous amino acid additions
relative to amino acids 9-50, amino acids 55-91, or amino acids
95-146 of SEQ ID NO: 22. In other aspects of this embodiment, a
Clostridial botulinum serotype D HA-17 comprising a .beta.-trefoil
domain with enhanced binding activity comprises a polypeptide
having, e.g., at least one, two, three, four, five, six, seven,
eight, nine, 10 or 20 non-contiguous amino acid additions relative
to amino acids 9-50, amino acids 55-91, or amino acids 95-146 of
SEQ ID NO: 22.
[0489] In other aspects of this embodiment, a Clostridial botulinum
serotype D HA-17 comprising a .beta.-trefoil domain with enhanced
binding activity comprises a polypeptide having, e.g., at most one,
two, three, four, five, six, seven, eight, nine, 10 or 20
contiguous amino acid substitutions relative to amino acids 9-50,
amino acids 55-91, or amino acids 95-146 of SEQ ID NO: 22. In other
aspects of this embodiment, a Clostridial botulinum serotype D
HA-17 comprising a .beta.-trefoil domain with enhanced binding
activity comprises a polypeptide having, e.g., at least one, two,
three, four, five, six, seven, eight, nine, 10 or 20 contiguous
amino acid substitutions relative to amino acids 9-50, amino acids
55-91, or amino acids 95-146 of SEQ ID NO: 22. In yet other aspects
of this embodiment, a Clostridial botulinum serotype D HA-17
comprising a .beta.-trefoil domain with enhanced binding activity
comprises a polypeptide having, e.g., at most one, two, three,
four, five, six, seven, eight, nine, 10 or 20 contiguous amino acid
deletions relative to amino acids 9-50, amino acids 55-91, or amino
acids 95-146 of SEQ ID NO: 22. In other aspects of this embodiment,
a Clostridial botulinum serotype D HA-17 comprising a
.beta.-trefoil domain with enhanced binding activity comprises a
polypeptide having, e.g., at least one, two, three, four, five,
six, seven, eight, nine, 10 or 20 contiguous amino acid deletions
relative to amino acids 9-50, amino acids 55-91, or amino acids
95-146 of SEQ ID NO: 22. In still other aspects of this embodiment,
a Clostridial botulinum serotype D HA-17 comprising a
.beta.-trefoil domain with enhanced binding activity comprises a
polypeptide having, e.g., at most one, two, three, four, five, six,
seven, eight, nine, 10 or 20 contiguous amino acid additions
relative to amino acids 9-50, amino acids 55-91, or amino acids
95-146 of SEQ ID NO: 22. In other aspects of this embodiment, a
Clostridial botulinum serotype D HA-17 comprising a .beta.-trefoil
domain with enhanced binding activity comprises a polypeptide
having, e.g., at least one, two, three, four, five, six, seven,
eight, nine, 10 or 20 contiguous amino acid additions relative to
amino acids 9-50, amino acids 55-91, or amino acids 95-146 of SEQ
ID NO: 22.
[0490] In another embodiment, a .beta.-trefoil domain derived from
a Clostridial botulinum serotype D HA-17 comprises a
.beta.4/.beta.5 hairpin turn of a .beta.-trefoil domain of a
Clostridial botulinum serotype D HA-17 or a .beta.8/.beta.9 hairpin
turn of a .beta.-trefoil domain of a Clostridial botulinum serotype
D HA-17 of SEQ ID NO: 22. In another aspect of this embodiment, a
.beta.-trefoil domain derived from a Clostridial botulinum serotype
D HA-17 comprises amino acids 51-54 or amino acids 92-94 of SEQ ID
NO: 22.
[0491] In another embodiment, a .beta.-trefoil domain with enhanced
binding activity derived from a Clostridial botulinum serotype D
HA-17 comprises a modified .beta.4/.beta.5 hairpin turn of a
.beta.-trefoil domain of a Clostridial botulinum serotype D HA-17
or a modified .beta.8/.beta.9 hairpin turn of a .beta.-trefoil
domain of a Clostridial botulinum serotype D HA-17 of SEQ ID NO:
22. In another aspect of this embodiment, a .beta.-trefoil domain
with enhanced binding activity derived from a Clostridial botulinum
serotype D HA-17 comprises a modification of amino acids 51-54 or
amino acids 92-94 of SEQ ID NO: 22.
[0492] In other aspects of this embodiment, a .beta.-trefoil domain
with enhanced binding activity derived from a Clostridial botulinum
serotype D HA-17 comprises a polypeptide having, e.g., at least 70%
amino acid identity with amino acids 51-54 or amino acids 92-94 of
SEQ ID NO: 22, at least 75% amino acid identity with amino acids
51-54 or amino acids 92-94 of SEQ ID NO: 22, at least 80% amino
acid identity with amino acids 51-54 or amino acids 92-94 of SEQ ID
NO: 22, at least 85% amino acid identity with amino acids 51-54 or
amino acids 92-94 of SEQ ID NO: 22, at least 90% amino acid
identity with amino acids 51-54 or amino acids 92-94 of SEQ ID NO:
22 or at least 95% amino acid identity with amino acids 51-54 or
amino acids 92-94 of SEQ ID NO: 22. In yet other aspects of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a Clostridial botulinum serotype D HA-17 comprises a
polypeptide having, e.g., at most 70% amino acid identity with
amino acids 51-54 or amino acids 92-94 of SEQ ID NO: 22, at most
75% amino acid identity with amino acids 51-54 or amino acids 92-94
of SEQ ID NO: 22, at most 80% amino acid identity with amino acids
51-54 or amino acids 92-94 of SEQ ID NO: 22, at most 85% amino acid
identity with amino acids 51-54 or amino acids 92-94 of SEQ ID NO:
22, at most 90% amino acid identity with amino acids 51-54 or amino
acids 92-94 of SEQ ID NO: 22 or at most 95% amino acid identity
with amino acids 51-54 or amino acids 92-94 of SEQ ID NO: 22.
[0493] In other aspects of this embodiment, a .beta.-trefoil domain
with enhanced binding activity derived from a Clostridial botulinum
serotype D HA-17 comprises a polypeptide having, e.g., at most one,
two, three or four non-contiguous amino acid substitutions relative
to amino acids 51-54 or amino acids 92-94 of SEQ ID NO: 22. In
other aspects of this embodiment, a .beta.-trefoil domain with
enhanced binding activity derived from a Clostridial botulinum
serotype D HA-17 comprises a polypeptide having, e.g., at least
one, two, three or four non-contiguous amino acid substitutions
relative to amino acids 51-54 or amino acids 92-94 of SEQ ID NO:
22. In other aspects of this embodiment, a non-contiguous amino
acid substitution of any amino acid from amino acids 51-54 or amino
acids 92-94 of SEQ ID NO: 22 can be replaced with glycine. In other
aspects of this embodiment, a non-contiguous amino acid
substitution of any hydrophobic amino acid from amino acids 51-54
or amino acids 92-94 of SEQ ID NO: 22 can be replaced with
phenylalanine. In yet other aspects of this embodiment, a
.beta.-trefoil domain with enhanced binding activity derived from a
Clostridial botulinum serotype D HA-17 comprises a polypeptide
having, e.g., at most one, two, three or four non-contiguous amino
acid deletions relative to amino acids 51-54 or amino acids 92-94
of SEQ ID NO: 22. In other aspects of this embodiment, a
.beta.-trefoil domain with enhanced binding activity derived from a
Clostridial botulinum serotype D HA-17 comprises a polypeptide
having, e.g., at least one, two, three or four non-contiguous amino
acid deletions relative to amino acids 51-54 or amino acids 92-94
of SEQ ID NO: 22. In still other aspects of this embodiment, a
.beta.-trefoil domain with enhanced binding activity derived from a
Clostridial botulinum serotype D HA-17 comprises a polypeptide
having, e.g., at most one, two, three or four non-contiguous amino
acid additions relative to amino acids 51-54 or amino acids 92-94
of SEQ ID NO: 22. In other aspects of this embodiment, a
.beta.-trefoil domain with enhanced binding activity derived from a
Clostridial botulinum serotype D HA-17 comprises a polypeptide
having, e.g., at least one, two, three or four non-contiguous amino
acid additions relative to amino acids 51-54 or amino acids 92-94
of SEQ ID NO: 22.
[0494] In other aspects of this embodiment, a .beta.-trefoil domain
with enhanced binding activity derived from a Clostridial botulinum
serotype D HA-17 comprises a polypeptide having, e.g., at most one,
two, three or four contiguous amino acid substitutions relative to
amino acids 51-54 or amino acids 92-94 of SEQ ID NO: 22. In other
aspects of this embodiment, a .beta.-trefoil domain with enhanced
binding activity derived from a Clostridial botulinum serotype D
HA-17 comprises a polypeptide having, e.g., at least one, two,
three or four contiguous amino acid substitutions relative to amino
acids 51-54 or amino acids 92-94 of SEQ ID NO: 22. In other aspects
of this embodiment, contiguous amino acid substitutions of amino
acids from amino acids 51-54 or amino acids 92-94 of SEQ ID NO: 22
can be replaced with glycine. In other aspects of this embodiment,
contiguous amino acid substitutions of hydrophobic amino acids from
amino acids 51-54 or amino acids 92-94 of SEQ ID NO: 22 can be
replaced with phenylalanine. In yet other aspects of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a Clostridial botulinum serotype D HA-17 comprises a
polypeptide having, e.g., at most one, two, three or four
contiguous amino acid deletions relative to amino acids 51-54 or
amino acids 92-94 of SEQ ID NO: 22. In other aspects of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a Clostridial botulinum serotype D HA-17 comprises a
polypeptide having, e.g., at least one, two, three or four
contiguous amino acid deletions relative to amino acids 51-54 or
amino acids 92-94 of SEQ ID NO: 22. In still other aspects of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a Clostridial botulinum serotype D HA-17 comprises a
polypeptide having, e.g., at most one, two, three or four
contiguous amino acid additions relative to amino acids 51-54 or
amino acids 92-94 of SEQ ID NO: 22. In other aspects of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a Clostridial botulinum serotype D HA-17 comprises a
polypeptide having, e.g., at least one, two, three or four
contiguous amino acid additions relative to amino acids 51-54 or
amino acids 92-94 of SEQ ID NO: 22.
[0495] In an embodiment, a modified Clostridial toxin disclosed in
the present specification comprises an enhanced targeting domain
comprising a .beta.-trefoil domain derived from a Clostridial NTNH.
In an aspect of this embodiment, a .beta.-trefoil domain derived
from a Clostridial NTNH comprises, e.g., a .beta.-trefoil domain
derived from a Clostridial botulinum serotype A NTNH, a
.beta.-trefoil domain derived from a Clostridial botulinum serotype
B NTNH, a .beta.-trefoil domain derived from a Clostridial
botulinum serotype C1 NTNH, a .beta.-trefoil domain derived from a
Clostridial botulinum serotype D NTNH, a .beta.-trefoil domain
derived from a Clostridial botulinum serotype E NTNH, a
.beta.-trefoil domain derived from a Clostridial botulinum serotype
F NTNH or a .beta.-trefoil domain derived from a Clostridial
botulinum serotype G NTNH. In another aspect of this embodiment, a
.beta.-trefoil domain derived from a Clostridial NTNH comprises a
.alpha.-fold motif of a .beta.-trefoil domain of a Clostridial
NTNH, a .beta.-fold motif of a .beta.-trefoil domain of a
Clostridial NTNH or a .gamma.-fold motif of a .beta.-trefoil domain
of a Clostridial NTNH.
[0496] In an embodiment, a modified Clostridial toxin disclosed in
the present specification comprises an enhanced targeting domain
comprising a .beta.-trefoil domain with enhanced binding activity
derived from a Clostridial NTNH. In an aspect of this embodiment, a
.beta.-trefoil domain with enhanced binding activity derived from a
Clostridial NTNH comprises, e.g., a .beta.-trefoil domain with
enhanced binding activity derived from a Clostridial botulinum
serotype A NTNH, a .beta.-trefoil domain with enhanced binding
activity derived from a Clostridial botulinum serotype B NTNH, a
.beta.-trefoil domain with enhanced binding activity derived from a
Clostridial botulinum serotype C1 NTNH, a .beta.-trefoil domain
with enhanced binding activity derived from a Clostridial botulinum
serotype D NTNH, a .beta.-trefoil domain with enhanced binding
activity derived from a Clostridial botulinum serotype E NTNH, a
.beta.-trefoil domain with enhanced binding activity derived from a
Clostridial botulinum serotype F NTNH or, a .beta.-trefoil domain
with enhanced binding activity derived from a Clostridial botulinum
serotype G NTNH. In another aspect of this embodiment, a
.beta.-trefoil domain with enhanced binding activity derived from a
Clostridial NTNH comprises a modified .alpha.-fold motif of a
.beta.-trefoil domain of a Clostridial NTNH, a modified .beta.-fold
motif of a .beta.-trefoil domain of a Clostridial NTNH or a
modified .gamma.-fold motif of a .beta.-trefoil domain of a
Clostridial NTNH.
[0497] In another embodiment, a .beta.-trefoil domain derived from
a Clostridial botulinum serotype A NTNH comprises a .beta.-trefoil
domain derived from a Clostridial botulinum serotype A NTNH of SEQ
ID NO: 23. In another embodiment, a .beta.-trefoil domain derived
from a Clostridial botulinum serotype A NTNH comprises amino acids
1050-1194 of SEQ ID NO: 23. In another aspect of this embodiment, a
.beta.-trefoil domain derived from a Clostridial botulinum serotype
A NTNH comprises a .alpha.-fold motif of a .beta.-trefoil domain of
a Clostridial botulinum serotype A NTNH, a .beta.-fold motif of a
.beta.-trefoil domain of a Clostridial botulinum serotype A NTNH or
a .gamma.-fold motif of a .beta.-trefoil domain of a Clostridial
botulinum serotype A NTNH of SEQ ID NO: 23. In another aspect of
this embodiment, a .beta.-trefoil domain derived from a Clostridial
botulinum serotype A NTNH comprises amino acids 1050-1097, amino
acids 1111-1138, or amino acids 1149-1194 of SEQ ID NO: 23.
[0498] In another embodiment, a .beta.-trefoil domain with enhanced
binding activity derived from a Clostridial botulinum serotype A
NTNH comprises a .beta.-trefoil domain with enhanced binding
activity derived from a Clostridial botulinum serotype A NTNH of
SEQ ID NO: 23. In another embodiment, a .beta.-trefoil domain with
enhanced binding activity derived from a Clostridial botulinum
serotype A NTNH comprises amino acids 1050-1194 of SEQ ID NO: 23.
In another aspect of this embodiment, a .beta.-trefoil domain with
enhanced binding activity derived from a Clostridial botulinum
serotype A NTNH comprises a modified .alpha.-fold motif of a
.beta.-trefoil domain of a Clostridial botulinum serotype A NTNH, a
modified .beta.-fold motif of a .beta.-trefoil domain of a
Clostridial botulinum serotype A NTNH or a modified .gamma.-fold
motif of a .beta.-trefoil domain of a Clostridial botulinum
serotype A NTNH of SEQ ID NO: 23. In another aspect of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a Clostridial botulinum serotype A NTNH comprises
amino acids 1050-1097, amino acids 1111-1138, or amino acids
1149-1194 of SEQ ID NO: 23.
[0499] In other aspects of this embodiment, a Clostridial botulinum
serotype A NTNH comprising a .beta.-trefoil domain with enhanced
binding activity comprises a polypeptide having, e.g., at least 70%
amino acid identity with amino acids 1050-1097, amino acids
1111-1138, or amino acids 1149-1194 of SEQ ID NO: 23, at least 75%
amino acid identity with amino acids 1050-1097, amino acids
1111-1138, or amino acids 1149-1194 of SEQ ID NO: 23, at least 80%
amino acid identity with amino acids 1050-1097, amino acids
1111-1138, or amino acids 1149-1194 of SEQ ID NO: 23, at least 85%
amino acid identity with amino acids 1050-1097, amino acids
1111-1138, or amino acids 1149-1194 of SEQ ID NO: 23, at least 90%
amino acid identity with amino acids 1050-1097, amino acids
1111-1138, or amino acids 1149-1194 of SEQ ID NO: 23 or at least
95% amino acid identity with amino acids 1050-1097, amino acids
1111-1138, or amino acids 1149-1194 of SEQ ID NO: 23. In yet other
aspects of this embodiment, a Clostridial botulinum serotype A NTNH
comprising a .beta.-trefoil domain with enhanced binding activity
comprises a polypeptide having, e.g., at most 70% amino acid
identity with amino acids 1050-1097, amino acids 1111-1138, or
amino acids 1149-1194 of SEQ ID NO: 23, at most 75% amino acid
identity with amino acids 1050-1097, amino acids 1111-1138, or
amino acids 1149-1194 of SEQ ID NO: 23, at most 80% amino acid
identity with amino acids 1050-1097, amino acids 1111-1138, or
amino acids 1149-1194 of SEQ ID NO: 23, at most 85% amino acid
identity with amino acids 1050-1097, amino acids 1111-1138, or
amino acids 1149-1194 of SEQ ID NO: 23, at most 90% amino acid
identity with amino acids 1050-1097, amino acids 1111-1138, or
amino acids 1149-1194 of SEQ ID NO: 23 or at most 95% amino acid
identity with amino acids 1050-1097, amino acids 1111-1138, or
amino acids 1149-1194 of SEQ ID NO: 23.
[0500] In other aspects of this embodiment, a Clostridial botulinum
serotype A NTNH comprising a .beta.-trefoil domain with enhanced
binding activity comprises a polypeptide having, e.g., at most one,
two, three, four, five, six, seven, eight, nine, 10 or 20
non-contiguous amino acid substitutions relative to amino acids
1050-1097, amino acids 1111-1138, or amino acids 1149-1194 of SEQ
ID NO: 23. In other aspects of this embodiment, a Clostridial
botulinum serotype A NTNH comprising a .beta.-trefoil domain with
enhanced binding activity comprises a polypeptide having, e.g., at
least one, two, three, four, five, six, seven, eight, nine, 10 or
20 non-contiguous amino acid substitutions relative to amino acids
1050-1097, amino acids 1111-1138, or amino acids 1149-1194 of SEQ
ID NO: 23. In yet other aspects of this embodiment, a Clostridial
botulinum serotype A NTNH comprising a .beta.-trefoil domain with
enhanced binding activity comprises a polypeptide having, e.g., at
most one, two, three, four, five, six, seven, eight, nine, 10 or 20
non-contiguous amino acid deletions relative to amino acids
1050-1097, amino acids 1111-1138, or amino acids 1149-1194 of SEQ
ID NO: 23. In other aspects of this embodiment, a Clostridial
botulinum serotype A NTNH comprising a .beta.-trefoil domain with
enhanced binding activity comprises a polypeptide having, e.g., at
least one, two, three, four, five, six, seven, eight, nine, 10 or
20 non-contiguous amino acid deletions relative to amino acids
1050-1097, amino acids 1111-1138, or amino acids 1149-1194 of SEQ
ID NO: 23. In still other aspects of this embodiment, a Clostridial
botulinum serotype A NTNH comprising a .beta.-trefoil domain with
enhanced binding activity comprises a polypeptide having, e.g., at
most one, two, three, four, five, six, seven, eight, nine, 10 or 20
non-contiguous amino acid additions relative to amino acids
1050-1097, amino acids 1111-1138, or amino acids 1149-1194 of SEQ
ID NO: 23. In other aspects of this embodiment, a Clostridial
botulinum serotype A NTNH comprising a .beta.-trefoil domain with
enhanced binding activity comprises a polypeptide having, e.g., at
least one, two, three, four, five, six, seven, eight, nine, 10 or
20 non-contiguous amino acid additions relative to amino acids
1050-1097, amino acids 1111-1138, or amino acids 1149-1194 of SEQ
ID NO: 23.
[0501] In other aspects of this embodiment, a Clostridial botulinum
serotype A NTNH comprising a .beta.-trefoil domain with enhanced
binding activity comprises a polypeptide having, e.g., at most one,
two, three, four, five, six, seven, eight, nine, 10 or 20
contiguous amino acid substitutions relative to amino acids
1050-1097, amino acids 1111-1138, or amino acids 1149-1194 of SEQ
ID NO: 23. In other aspects of this embodiment, a Clostridial
botulinum serotype A NTNH comprising a .beta.-trefoil domain with
enhanced binding activity comprises a polypeptide having, e.g., at
least one, two, three, four, five, six, seven, eight, nine, 10 or
20 contiguous amino acid substitutions relative to amino acids
1050-1097, amino acids 1111-1138, or amino acids 1149-1194 of SEQ
ID NO: 23. In yet other aspects of this embodiment, a Clostridial
botulinum serotype A NTNH comprising a .beta.-trefoil domain with
enhanced binding activity comprises a polypeptide having, e.g., at
most one, two, three, four, five, six, seven, eight, nine, 10 or 20
contiguous amino acid deletions relative to amino acids 1050-1097,
amino acids 1111-1138, or amino acids 1149-1194 of SEQ ID NO: 23.
In other aspects of this embodiment, a Clostridial botulinum
serotype A NTNH comprising a .beta.-trefoil domain with enhanced
binding activity comprises a polypeptide having, e.g., at least
one, two, three, four, five, six, seven, eight, nine, 10 or 20
contiguous amino acid deletions relative to amino acids 1050-1097,
amino acids 1111-1138, or amino acids 1149-1194 of SEQ ID NO: 23.
In still other aspects of this embodiment, a Clostridial botulinum
serotype A NTNH comprising a .beta.-trefoil domain with enhanced
binding activity comprises a polypeptide having, e.g., at most one,
two, three, four, five, six, seven, eight, nine, 10 or 20
contiguous amino acid additions relative to amino acids 1050-1097,
amino acids 1111-1138, or amino acids 1149-1194 of SEQ ID NO: 23.
In other aspects of this embodiment, a Clostridial botulinum
serotype A NTNH comprising a .beta.-trefoil domain with enhanced
binding activity comprises a polypeptide having, e.g., at least
one, two, three, four, five, six, seven, eight, nine, 10 or 20
contiguous amino acid additions relative to amino acids 1050-1097,
amino acids 1111-1138, or amino acids 1149-1194 of SEQ ID NO:
23.
[0502] In another embodiment, a .beta.-trefoil domain derived from
a Clostridial botulinum serotype A NTNH comprises a .beta.4/.beta.5
hairpin turn of a .beta.-trefoil domain of a Clostridial botulinum
serotype A NTNH or a .beta.8/.beta.9 hairpin turn of a
.beta.-trefoil domain of a Clostridial botulinum serotype A NTNH of
SEQ ID NO: 23. In another aspect of this embodiment, a
.beta.-trefoil domain derived from a Clostridial botulinum serotype
A NTNH comprises amino acids 1098-1110 or amino acids 1139-1148 of
SEQ ID NO: 23.
[0503] In another embodiment, a .beta.-trefoil domain with enhanced
binding activity derived from a Clostridial botulinum serotype A
NTNH comprises a modified .beta.4/.beta.5 hairpin turn of a
.beta.-trefoil domain of a Clostridial botulinum serotype A NTNH or
a modified .beta.8/.beta.9 hairpin turn of a .beta.-trefoil domain
of a Clostridial botulinum serotype A NTNH of SEQ ID NO: 23. In
another aspect of this embodiment, a .beta.-trefoil domain with
enhanced binding activity derived from a Clostridial botulinum
serotype A NTNH comprises a modification of amino acids 1098-1110
or amino acids 1139-1148 of SEQ ID NO: 23.
[0504] In other aspects of this embodiment, a .beta.-trefoil domain
with enhanced binding activity derived from a Clostridial botulinum
serotype A NTNH comprises a polypeptide having, e.g., at least 70%
amino acid identity with amino acids 1098-1110 or amino acids
1139-1148 of SEQ ID NO: 23, at least 75% amino acid identity with
amino acids 1098-1110 or amino acids 1139-1148 of SEQ ID NO: 23, at
least 80% amino acid identity with amino acids 1098-1110 or amino
acids 1139-1148 of SEQ ID NO: 23, at least 85% amino acid identity
with amino acids 1098-1110 or amino acids 1139-1148 of SEQ ID NO:
23, at least 90% amino acid identity with amino acids 1098-1110 or
amino acids 1139-1148 of SEQ ID NO: 23 or at least 95% amino acid
identity with amino acids 1098-1110 or amino acids 1139-1148 of SEQ
ID NO: 23. In yet other aspects of this embodiment, a
.beta.-trefoil domain with enhanced binding activity derived from a
Clostridial botulinum serotype A NTNH comprises a polypeptide
having, e.g., at most 70% amino acid identity with amino acids
1098-1110 or amino acids 1139-1148 of SEQ ID NO: 23, at most 75%
amino acid identity with amino acids 1098-1110 or amino acids
1139-1148 of SEQ ID NO: 23, at most 80% amino acid identity with
amino acids 1098-1110 or amino acids 1139-1148 of SEQ ID NO: 23, at
most 85% amino acid identity with amino acids 1098-1110 or amino
acids 1139-1148 of SEQ ID NO: 23, at most 90% amino acid identity
with amino acids 1098-1110 or amino acids 1139-1148 of SEQ ID NO:
23 or at most 95% amino acid identity with amino acids 1098-1110 or
amino acids 1139-1148 of SEQ ID NO: 23.
[0505] In other aspects of this embodiment, a .beta.-trefoil domain
with enhanced binding activity derived from a Clostridial botulinum
serotype A NTNH comprises a polypeptide having, e.g., at most one,
two, three or four non-contiguous amino acid substitutions relative
to amino acids 1098-1110 or amino acids 1139-1148 of SEQ ID NO: 23.
In other aspects of this embodiment, a .beta.-trefoil domain with
enhanced binding activity derived from a Clostridial botulinum
serotype A NTNH comprises a polypeptide having, e.g., at least one,
two, three or four non-contiguous amino acid substitutions relative
to amino acids 1098-1110 or amino acids 1139-1148 of SEQ ID NO: 23.
In other aspects of this embodiment, a non-contiguous amino acid
substitution of any amino acid from amino acids 1098-1110 or amino
acids 1139-1148 of SEQ ID NO: 23 can be replaced with glycine. In
other aspects of this embodiment, a non-contiguous amino acid
substitution of any hydrophobic amino acid from amino acids
1098-1110 or amino acids 1139-1148 of SEQ ID NO: 23 can be replaced
with phenylalanine. In yet other aspects of this embodiment, a
.beta.-trefoil domain with enhanced binding activity derived from a
Clostridial botulinum serotype A NTNH comprises a polypeptide
having, e.g., at most one, two, three or four non-contiguous amino
acid deletions relative to amino acids 1098-1110 or amino acids
1139-1148 of SEQ ID NO: 23. In other aspects of this embodiment, a
.beta.-trefoil domain with enhanced binding activity derived from a
Clostridial botulinum serotype A NTNH comprises a polypeptide
having, e.g., at least one, two, three or four non-contiguous amino
acid deletions relative to amino acids 1098-1110 or amino acids
1139-1148 of SEQ ID NO: 23. In still other aspects of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a Clostridial botulinum serotype A NTNH comprises a
polypeptide having, e.g., at most one, two, three or four
non-contiguous amino acid additions relative to amino acids
1098-1110 or amino acids 1139-1148 of SEQ ID NO: 23. In other
aspects of this embodiment, a .beta.-trefoil domain with enhanced
binding activity derived from a Clostridial botulinum serotype A
NTNH comprises a polypeptide having, e.g., at least one, two, three
or four non-contiguous amino acid additions relative to amino acids
1098-1110 or amino acids 1139-1148 of SEQ ID NO: 23.
[0506] In other aspects of this embodiment, a .beta.-trefoil domain
with enhanced binding activity derived from a Clostridial botulinum
serotype A NTNH comprises a polypeptide having, e.g., at most one,
two, three or four contiguous amino acid substitutions relative to
amino acids 1098-1110 or amino acids 1139-1148 of SEQ ID NO: 23. In
other aspects of this embodiment, a .beta.-trefoil domain with
enhanced binding activity derived from a Clostridial botulinum
serotype A NTNH comprises a polypeptide having, e.g., at least one,
two, three or four contiguous amino acid substitutions relative to
amino acids 1098-1110 or amino acids 1139-1148 of SEQ ID NO: 23. In
other aspects of this embodiment, contiguous amino acid
substitutions of amino acids from amino acids 1098-1110 or amino
acids 1139-1148 of SEQ ID NO: 23 can be replaced with glycine. In
other aspects of this embodiment, contiguous amino acid
substitutions of hydrophobic amino acids from amino acids 1098-1110
or amino acids 1139-1148 of SEQ ID NO: 23 can be replaced with
phenylalanine. In yet other aspects of this embodiment, a
.beta.-trefoil domain with enhanced binding activity derived from a
Clostridial botulinum serotype A NTNH comprises a polypeptide
having, e.g., at most one, two, three or four contiguous amino acid
deletions relative to amino acids 1098-1110 or amino acids
1139-1148 of SEQ ID NO: 23. In other aspects of this embodiment, a
.beta.-trefoil domain with enhanced binding activity derived from a
Clostridial botulinum serotype A NTNH comprises a polypeptide
having, e.g., at least one, two, three or four contiguous amino
acid deletions relative to amino acids 1098-1110 or amino acids
1139-1148 of SEQ ID NO: 23. In still other aspects of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a Clostridial botulinum serotype A NTNH comprises a
polypeptide having, e.g., at most one, two, three or four
contiguous amino acid additions relative to amino acids 1098-1110
or amino acids 1139-1148 of SEQ ID NO: 23. In other aspects of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a Clostridial botulinum serotype A NTNH comprises a
polypeptide having, e.g., at least one, two, three or four
contiguous amino acid additions relative to amino acids 1098-1110
or amino acids 1139-1148 of SEQ ID NO: 23.
[0507] In another embodiment, a .beta.-trefoil domain derived from
a Clostridial botulinum serotype A NTNH comprises a .beta.-trefoil
domain derived from a Clostridial botulinum serotype A NTNH of SEQ
ID NO: 24. In another embodiment, a .beta.-trefoil domain derived
from a Clostridial botulinum serotype A NTNH comprises amino acids
1050-1194 of SEQ ID NO: 24. In another aspect of this embodiment, a
.beta.-trefoil domain derived from a Clostridial botulinum serotype
A NTNH comprises a .alpha.-fold motif of a .beta.-trefoil domain of
a Clostridial botulinum serotype A NTNH, a .beta.-fold motif of a
.beta.-trefoil domain of a Clostridial botulinum serotype A NTNH or
a .gamma.-fold motif of a .beta.-trefoil domain of a Clostridial
botulinum serotype A NTNH of SEQ ID NO: 24. In another aspect of
this embodiment, a .beta.-trefoil domain derived from a Clostridial
botulinum serotype A NTNH comprises amino acids 1050-1097, amino
acids 1111-1139, or amino acids 1149-1199 of SEQ ID NO: 24.
[0508] In another embodiment, a .beta.-trefoil domain with enhanced
binding activity derived from a Clostridial botulinum serotype A
NTNH comprises a .beta.-trefoil domain with enhanced binding
activity derived from a Clostridial botulinum serotype A NTNH of
SEQ ID NO: 24. In another embodiment, a .beta.-trefoil domain with
enhanced binding activity derived from a Clostridial botulinum
serotype A NTNH comprises amino acids 1050-1194 of SEQ ID NO: 24.
In another aspect of this embodiment, a .beta.-trefoil domain with
enhanced binding activity derived from a Clostridial botulinum
serotype A NTNH comprises a modified .alpha.-fold motif of a
.beta.-trefoil domain of a Clostridial botulinum serotype A NTNH, a
modified .beta.-fold motif of a .beta.-trefoil domain of a
Clostridial botulinum serotype A NTNH or a modified .gamma.-fold
motif of a 13-trefoil domain of a Clostridial botulinum serotype A
NTNH of SEQ ID NO: 24. In another aspect of this embodiment, a
.beta.-trefoil domain with enhanced binding activity derived from a
Clostridial botulinum serotype A NTNH comprises amino acids
1050-1097, amino acids 1111-1139, or amino acids 1149-1199 of SEQ
ID NO: 24.
[0509] In other aspects of this embodiment, a Clostridial botulinum
serotype A NTNH comprising a .beta.-trefoil domain with enhanced
binding activity comprises a polypeptide having, e.g., at least 70%
amino acid identity with amino acids 1050-1097, amino acids
1111-1139, or amino acids 1149-1199 of SEQ ID NO: 24, at least 75%
amino acid identity with amino acids 1050-1097, amino acids
1111-1139, or amino acids 1149-1199 of SEQ ID NO: 24, at least 80%
amino acid identity with amino acids 1050-1097, amino acids
1111-1139, or amino acids 1149-1199 of SEQ ID NO: 24, at least 85%
amino acid identity with amino acids 1050-1097, amino acids
1111-1139, or amino acids 1149-1199 of SEQ ID NO: 24, at least 90%
amino acid identity with amino acids 1050-1097, amino acids
1111-1139, or amino acids 1149-1199 of SEQ ID NO: 24 or at least
95% amino acid identity with amino acids 1050-1097, amino acids
1111-1139, or amino acids 1149-1199 of SEQ ID NO: 24. In yet other
aspects of this embodiment, a Clostridial botulinum serotype A NTNH
comprising a .beta.-trefoil domain with enhanced binding activity
comprises a polypeptide having, e.g., at most 70% amino acid
identity with amino acids 1050-1097, amino acids 1111-1139, or
amino acids 1149-1199 of SEQ ID NO: 24, at most 75% amino acid
identity with amino acids 1050-1097, amino acids 1111-1139, or
amino acids 1149-1199 of SEQ ID NO: 24, at most 80% amino acid
identity with amino acids 1050-1097, amino acids 1111-1139, or
amino acids 1149-1199 of SEQ ID NO: 24, at most 85% amino acid
identity with amino acids 1050-1097, amino acids 1111-1139, or
amino acids 1149-1199 of SEQ ID NO: 24, at most 90% amino acid
identity with amino acids 1050-1097, amino acids 1111-1139, or
amino acids 1149-1199 of SEQ ID NO: 24 or at most 95% amino acid
identity with amino acids 1050-1097, amino acids 1111-1139, or
amino acids 1149-1199 of SEQ ID NO: 24.
[0510] In other aspects of this embodiment, a Clostridial botulinum
serotype A NTNH comprising a .beta.-trefoil domain with enhanced
binding activity comprises a polypeptide having, e.g., at most one,
two, three, four, five, six, seven, eight, nine, 10 or 20
non-contiguous amino acid substitutions relative to amino acids
1050-1097, amino acids 1111-1139, or amino acids 1149-1199 of SEQ
ID NO: 24. In other aspects of this embodiment, a Clostridial
botulinum serotype A NTNH comprising a .beta.-trefoil domain with
enhanced binding activity comprises a polypeptide having, e.g., at
least one, two, three, four, five, six, seven, eight, nine, 10 or
20 non-contiguous amino acid substitutions relative to amino acids
1050-1097, amino acids 1111-1139, or amino acids 1149-1199 of SEQ
ID NO: 24. In yet other aspects of this embodiment, a Clostridial
botulinum serotype A NTNH comprising a .beta.-trefoil domain with
enhanced binding activity comprises a polypeptide having, e.g., at
most one, two, three, four, five, six, seven, eight, nine, 10 or 20
non-contiguous amino acid deletions relative to amino acids
1050-1097, amino acids 1111-1139, or amino acids 1149-1199 of SEQ
ID NO: 24. In other aspects of this embodiment, a Clostridial
botulinum serotype A NTNH comprising a .beta.-trefoil domain with
enhanced binding activity comprises a polypeptide having, e.g., at
least one, two, three, four, five, six, seven, eight, nine, 10 or
20 non-contiguous amino acid deletions relative to amino acids
1050-1097, amino acids 1111-1139, or amino acids 1149-1199 of SEQ
ID NO: 24. In still other aspects of this embodiment, a Clostridial
botulinum serotype A NTNH comprising a .beta.-trefoil domain with
enhanced binding activity comprises a polypeptide having, e.g., at
most one, two, three, four, five, six, seven, eight, nine, 10 or 20
non-contiguous amino acid additions relative to amino acids
1050-1097, amino acids 1111-1139, or amino acids 1149-1199 of SEQ
ID NO: 24. In other aspects of this embodiment, a Clostridial
botulinum serotype A NTNH comprising a .beta.-trefoil domain with
enhanced binding activity comprises a polypeptide having, e.g., at
least one, two, three, four, five, six, seven, eight, nine, 10 or
20 non-contiguous amino acid additions relative to amino acids
1050-1097, amino acids 1111-1139, or amino acids 1149-1199 of SEQ
ID NO: 24.
[0511] In other aspects of this embodiment, a Clostridial botulinum
serotype A NTNH comprising a .beta.-trefoil domain with enhanced
binding activity comprises a polypeptide having, e.g., at most one,
two, three, four, five, six, seven, eight, nine, 10 or 20
contiguous amino acid substitutions relative to amino acids
1050-1097, amino acids 1111-1139, or amino acids 1149-1199 of SEQ
ID NO: 24. In other aspects of this embodiment, a Clostridial
botulinum serotype A NTNH comprising a .beta.-trefoil domain with
enhanced binding activity comprises a polypeptide having, e.g., at
least one, two, three, four, five, six, seven, eight, nine, 10 or
20 contiguous amino acid substitutions relative to amino acids
1050-1097, amino acids 1111-1139, or amino acids 1149-1199 of SEQ
ID NO: 24. In yet other aspects of this embodiment, a Clostridial
botulinum serotype A NTNH comprising a .beta.-trefoil domain with
enhanced binding activity comprises a polypeptide having, e.g., at
most one, two, three, four, five, six, seven, eight, nine, 10 or 20
contiguous amino acid deletions relative to amino acids 1050-1097,
amino acids 1111-1139, or amino acids 1149-1199 of SEQ ID NO: 24.
In other aspects of this embodiment, a Clostridial botulinum
serotype A NTNH comprising a .beta.-trefoil domain with enhanced
binding activity comprises a polypeptide having, e.g., at least
one, two, three, four, five, six, seven, eight, nine, 10 or 20
contiguous amino acid deletions relative to amino acids 1050-1097,
amino acids 1111-1139, or amino acids 1149-1199 of SEQ ID NO: 24.
In still other aspects of this embodiment, a Clostridial botulinum
serotype A NTNH comprising a .beta.-trefoil domain with enhanced
binding activity comprises a polypeptide having, e.g., at most one,
two, three, four, five, six, seven, eight, nine, 10 or 20
contiguous amino acid additions relative to amino acids 1050-1097,
amino acids 1111-1139, or amino acids 1149-1199 of SEQ ID NO: 24.
In other aspects of this embodiment, a Clostridial botulinum
serotype A NTNH comprising a .beta.-trefoil domain with enhanced
binding activity comprises a polypeptide having, e.g., at least
one, two, three, four, five, six, seven, eight, nine, 10 or 20
contiguous amino acid additions relative to amino acids 1050-1097,
amino acids 1111-1139, or amino acids 1149-1199 of SEQ ID NO:
24.
[0512] In another embodiment, a .beta.-trefoil domain derived from
a Clostridial botulinum serotype A NTNH comprises a .beta.4/.beta.5
hairpin turn of a .beta.-trefoil domain of a Clostridial botulinum
serotype A NTNH or a 138/139 hairpin turn of a .beta.-trefoil
domain of a Clostridial botulinum serotype A NTNH of SEQ ID NO: 24.
In another aspect of this embodiment, a .beta.-trefoil domain
derived from a Clostridial botulinum serotype A NTNH comprises
amino acids 1098-1110 or amino acids 1140-1148 of SEQ ID NO:
24.
[0513] In another embodiment, a .beta.-trefoil domain with enhanced
binding activity derived from a Clostridial botulinum serotype A
NTNH comprises a modified .beta.4/.beta.5 hairpin turn of a
.beta.-trefoil domain of a Clostridial botulinum serotype A NTNH or
a modified .beta.8/.beta.9 hairpin turn of a .beta.-trefoil domain
of a Clostridial botulinum serotype A NTNH of SEQ ID NO: 24. In
another aspect of this embodiment, a .beta.-trefoil domain with
enhanced binding activity derived from a Clostridial botulinum
serotype A NTNH comprises a modification of amino acids 1098-1110
or amino acids 1140-1148 of SEQ ID NO: 24.
[0514] In other aspects of this embodiment, a .beta.-trefoil domain
with enhanced binding activity derived from a Clostridial botulinum
serotype A NTNH comprises a polypeptide having, e.g., at least 70%
amino acid identity with amino acids 1098-1110 or amino acids
1140-1148 of SEQ ID NO: 24, at least 75% amino acid identity with
amino acids 1098-1110 or amino acids 1140-1148 of SEQ ID NO: 24, at
least 80% amino acid identity with amino acids 1098-1110 or amino
acids 1140-1148 of SEQ ID NO: 24, at least 85% amino acid identity
with amino acids 1098-1110 or amino acids 1140-1148 of SEQ ID NO:
24, at least 90% amino acid identity with amino acids 1098-1110 or
amino acids 1140-1148 of SEQ ID NO: 24 or at least 95% amino acid
identity with amino acids 1098-1110 or amino acids 1140-1148 of SEQ
ID NO: 24. In yet other aspects of this embodiment, a
.beta.-trefoil domain with enhanced binding activity derived from a
Clostridial botulinum serotype A NTNH comprises a polypeptide
having, e.g., at most 70% amino acid identity with amino acids
1098-1110 or amino acids 1140-1148 of SEQ ID NO: 24, at most 75%
amino acid identity with amino acids 1098-1110 or amino acids
1140-1148 of SEQ ID NO: 24, at most 80% amino acid identity with
amino acids 1098-1110 or amino acids 1140-1148 of SEQ ID NO: 24, at
most 85% amino acid identity with amino acids 1098-1110 or amino
acids 1140-1148 of SEQ ID NO: 24, at most 90% amino acid identity
with amino acids 1098-1110 or amino acids 1140-1148 of SEQ ID NO:
24 or at most 95% amino acid identity with amino acids 1098-1110 or
amino acids 1140-1148 of SEQ ID NO: 24.
[0515] In other aspects of this embodiment, a .beta.-trefoil domain
with enhanced binding activity derived from a Clostridial botulinum
serotype A NTNH comprises a polypeptide having, e.g., at most one,
two, three or four non-contiguous amino acid substitutions relative
to amino acids 1098-1110 or amino acids 1140-1148 of SEQ ID NO: 24.
In other aspects of this embodiment, a .beta.-trefoil domain with
enhanced binding activity derived from a Clostridial botulinum
serotype A NTNH comprises a polypeptide having, e.g., at least one,
two, three or four non-contiguous amino acid substitutions relative
to amino acids 1098-1110 or amino acids 1140-1148 of SEQ ID NO: 24.
In other aspects of this embodiment, a non-contiguous amino acid
substitution of any amino acid from amino acids 1098-1110 or amino
acids 1140-1148 of SEQ ID NO: 24 can be replaced with glycine. In
other aspects of this embodiment, a non-contiguous amino acid
substitution of any hydrophobic amino acid from amino acids
1098-1110 or amino acids 1140-1148 of SEQ ID NO: 24 can be replaced
with phenylalanine. In yet other aspects of this embodiment, a
.beta.-trefoil domain with enhanced binding activity derived from a
Clostridial botulinum serotype A NTNH comprises a polypeptide
having, e.g., at most one, two, three or four non-contiguous amino
acid deletions relative to amino acids 1098-1110 or amino acids
1140-1148 of SEQ ID NO: 24. In other aspects of this embodiment, a
.beta.-trefoil domain with enhanced binding activity derived from a
Clostridial botulinum serotype A NTNH comprises a polypeptide
having, e.g., at least one, two, three or four non-contiguous amino
acid deletions relative to amino acids 1098-1110 or amino acids
1140-1148 of SEQ ID NO: 24. In still other aspects of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a Clostridial botulinum serotype A NTNH comprises a
polypeptide having, e.g., at most one, two, three or four
non-contiguous amino acid additions relative to amino acids
1098-1110 or amino acids 1140-1148 of SEQ ID NO: 24. In other
aspects of this embodiment, a .beta.-trefoil domain with enhanced
binding activity derived from a Clostridial botulinum serotype A
NTNH comprises a polypeptide having, e.g., at least one, two, three
or four non-contiguous amino acid additions relative to amino acids
1098-1110 or amino acids 1140-1148 of SEQ ID NO: 24.
[0516] In other aspects of this embodiment, a .beta.-trefoil domain
with enhanced binding activity derived from a Clostridial botulinum
serotype A NTNH comprises a polypeptide having, e.g., at most one,
two, three or four contiguous amino acid substitutions relative to
amino acids 1098-1110 or amino acids 1140-1148 of SEQ ID NO: 24. In
other aspects of this embodiment, a .beta.-trefoil domain with
enhanced binding activity derived from a Clostridial botulinum
serotype A NTNH comprises a polypeptide having, e.g., at least one,
two, three or four contiguous amino acid substitutions relative to
amino acids 1098-1110 or amino acids 1140-1148 of SEQ ID NO: 24. In
other aspects of this embodiment, contiguous amino acid
substitutions of amino acids from amino acids 1098-1110 or amino
acids 1140-1148 of SEQ ID NO: 24 can be replaced with glycine. In
other aspects of this embodiment, contiguous amino acid
substitutions of hydrophobic amino acids from amino acids 1098-1110
or amino acids 1140-1148 of SEQ ID NO: 24 can be replaced with
phenylalanine. In yet other aspects of this embodiment, a
.beta.-trefoil domain with enhanced binding activity derived from a
Clostridial botulinum serotype A NTNH comprises a polypeptide
having, e.g., at most one, two, three or four contiguous amino acid
deletions relative to amino acids 1098-1110 or amino acids
1140-1148 of SEQ ID NO: 24. In other aspects of this embodiment, a
.beta.-trefoil domain with enhanced binding activity derived from a
Clostridial botulinum serotype A NTNH comprises a polypeptide
having, e.g., at least one, two, three or four contiguous amino
acid deletions relative to amino acids 1098-1110 or amino acids
1140-1148 of SEQ ID NO: 24. In still other aspects of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a Clostridial botulinum serotype A NTNH comprises a
polypeptide having, e.g., at most one, two, three or four
contiguous amino acid additions relative to amino acids 1098-1110
or amino acids 1140-1148 of SEQ ID NO: 24. In other aspects of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a Clostridial botulinum serotype A NTNH comprises a
polypeptide having, e.g., at least one, two, three or four
contiguous amino acid additions relative to amino acids 1098-1110
or amino acids 1140-1148 of SEQ ID NO: 24.
[0517] In another embodiment, a .beta.-trefoil domain derived from
a Clostridial botulinum serotype A NTNH comprises a .beta.-trefoil
domain derived from a Clostridial botulinum serotype A NTNH of SEQ
ID NO: 25. In another embodiment, a .beta.-trefoil domain derived
from a Clostridial botulinum serotype A NTNH comprises amino acids
1050-1194 of SEQ ID NO: 25. In another aspect of this embodiment, a
.beta.-trefoil domain derived from a Clostridial botulinum serotype
A NTNH comprises a .alpha.-fold motif of a .beta.-trefoil domain of
a Clostridial botulinum serotype A NTNH, a .beta.-fold motif of a
.beta.-trefoil domain of a Clostridial botulinum serotype A NTNH or
a .gamma.-fold motif of a .beta.-trefoil domain of a Clostridial
botulinum serotype A NTNH of SEQ ID NO: 25. In another aspect of
this embodiment, a .beta.-trefoil domain derived from a Clostridial
botulinum serotype A NTNH comprises amino acids 1050-1097, amino
acids 1111-1138, or amino acids 1149-1194 of SEQ ID NO: 25.
[0518] In another embodiment, a .beta.-trefoil domain with enhanced
binding activity derived from a Clostridial botulinum serotype A
NTNH comprises a .beta.-trefoil domain with enhanced binding
activity derived from a Clostridial botulinum serotype A NTNH of
SEQ ID NO: 25. In another embodiment, a .beta.-trefoil domain with
enhanced binding activity derived from a Clostridial botulinum
serotype A NTNH comprises amino acids 1050-1194 of SEQ ID NO: 25.
In another aspect of this embodiment, a .beta.-trefoil domain with
enhanced binding activity derived from a Clostridial botulinum
serotype A NTNH comprises a modified .alpha.-fold motif of a
.beta.-trefoil domain of a Clostridial botulinum serotype A NTNH, a
modified .beta.-fold motif of a .beta.-trefoil domain of a
Clostridial botulinum serotype A NTNH or a modified .gamma.-fold
motif of a 13-trefoil domain of a Clostridial botulinum serotype A
NTNH of SEQ ID NO: 25. In another aspect of this embodiment, a
.beta.-trefoil domain with enhanced binding activity derived from a
Clostridial botulinum serotype A NTNH comprises amino acids
1050-1097, amino acids 1111-1138, or amino acids 1149-1194 of SEQ
ID NO: 25.
[0519] In other aspects of this embodiment, a Clostridial botulinum
serotype A NTNH comprising a .beta.-trefoil domain with enhanced
binding activity comprises a polypeptide having, e.g., at least 70%
amino acid identity with amino acids 1050-1097, amino acids
1111-1138, or amino acids 1149-1194 of SEQ ID NO: 25, at least 75%
amino acid identity with amino acids 1050-1097, amino acids
1111-1138, or amino acids 1149-1194 of SEQ ID NO: 25, at least 80%
amino acid identity with amino acids 1050-1097, amino acids
1111-1138, or amino acids 1149-1194 of SEQ ID NO: 25, at least 85%
amino acid identity with amino acids 1050-1097, amino acids
1111-1138, or amino acids 1149-1194 of SEQ ID NO: 25, at least 90%
amino acid identity with amino acids 1050-1097, amino acids
1111-1138, or amino acids 1149-1194 of SEQ ID NO: 25 or at least
95% amino acid identity with amino acids 1050-1097, amino acids
1111-1138, or amino acids 1149-1194 of SEQ ID NO: 25. In yet other
aspects of this embodiment, a Clostridial botulinum serotype A NTNH
comprising a .beta.-trefoil domain with enhanced binding activity
comprises a polypeptide having, e.g., at most 70% amino acid
identity with amino acids 1050-1097, amino acids 1111-1138, or
amino acids 1149-1194 of SEQ ID NO: 25, at most 75% amino acid
identity with amino acids 1050-1097, amino acids 1111-1138, or
amino acids 1149-1194 of SEQ ID NO: 25, at most 80% amino acid
identity with amino acids 1050-1097, amino acids 1111-1138, or
amino acids 1149-1194 of SEQ ID NO: 25, at most 85% amino acid
identity with amino acids 1050-1097, amino acids 1111-1138, or
amino acids 1149-1194 of SEQ ID NO: 25, at most 90% amino acid
identity with amino acids 1050-1097, amino acids 1111-1138, or
amino acids 1149-1194 of SEQ ID NO: 25 or at most 95% amino acid
identity with amino acids 1050-1097, amino acids 1111-1138, or
amino acids 1149-1194 of SEQ ID NO: 25.
[0520] In other aspects of this embodiment, a Clostridial botulinum
serotype A NTNH comprising a .beta.-trefoil domain with enhanced
binding activity comprises a polypeptide having, e.g., at most one,
two, three, four, five, six, seven, eight, nine, 10 or 20
non-contiguous amino acid substitutions relative to amino acids
1050-1097, amino acids 1111-1138, or amino acids 1149-1194 of SEQ
ID NO: 25. In other aspects of this embodiment, a Clostridial
botulinum serotype A NTNH comprising a .beta.-trefoil domain with
enhanced binding activity comprises a polypeptide having, e.g., at
least one, two, three, four, five, six, seven, eight, nine, 10 or
20 non-contiguous amino acid substitutions relative to amino acids
1050-1097, amino acids 1111-1138, or amino acids 1149-1194 of SEQ
ID NO: 25. In yet other aspects of this embodiment, a Clostridial
botulinum serotype A NTNH comprising a .beta.-trefoil domain with
enhanced binding activity comprises a polypeptide having, e.g., at
most one, two, three, four, five, six, seven, eight, nine, 10 or 20
non-contiguous amino acid deletions relative to amino acids
1050-1097, amino acids 1111-1138, or amino acids 1149-1194 of SEQ
ID NO: 25. In other aspects of this embodiment, a Clostridial
botulinum serotype A NTNH comprising a .beta.-trefoil domain with
enhanced binding activity comprises a polypeptide having, e.g., at
least one, two, three, four, five, six, seven, eight, nine, 10 or
20 non-contiguous amino acid deletions relative to amino acids
1050-1097, amino acids 1111-1138, or amino acids 1149-1194 of SEQ
ID NO: 25. In still other aspects of this embodiment, a Clostridial
botulinum serotype A NTNH comprising a .beta.-trefoil domain with
enhanced binding activity comprises a polypeptide having, e.g., at
most one, two, three, four, five, six, seven, eight, nine, 10 or 20
non-contiguous amino acid additions relative to amino acids
1050-1097, amino acids 1111-1138, or amino acids 1149-1194 of SEQ
ID NO: 25. In other aspects of this embodiment, a Clostridial
botulinum serotype A NTNH comprising a .beta.-trefoil domain with
enhanced binding activity comprises a polypeptide having, e.g., at
least one, two, three, four, five, six, seven, eight, nine, 10 or
20 non-contiguous amino acid additions relative to amino acids
1050-1097, amino acids 1111-1138, or amino acids 1149-1194 of SEQ
ID NO: 25.
[0521] In other aspects of this embodiment, a Clostridial botulinum
serotype A NTNH comprising a .beta.-trefoil domain with enhanced
binding activity comprises a polypeptide having, e.g., at most one,
two, three, four, five, six, seven, eight, nine, 10 or 20
contiguous amino acid substitutions relative to amino acids
1050-1097, amino acids 1111-1138, or amino acids 1149-1194 of SEQ
ID NO: 25. In other aspects of this embodiment, a Clostridial
botulinum serotype A NTNH comprising a .beta.-trefoil domain with
enhanced binding activity comprises a polypeptide having, e.g., at
least one, two, three, four, five, six, seven, eight, nine, 10 or
20 contiguous amino acid substitutions relative to amino acids
1050-1097, amino acids 1111-1138, or amino acids 1149-1194 of SEQ
ID NO: 25. In yet other aspects of this embodiment, a Clostridial
botulinum serotype A NTNH comprising a .beta.-trefoil domain with
enhanced binding activity comprises a polypeptide having, e.g., at
most one, two, three, four, five, six, seven, eight, nine, 10 or 20
contiguous amino acid deletions relative to amino acids 1050-1097,
amino acids 1111-1138, or amino acids 1149-1194 of SEQ ID NO: 25.
In other aspects of this embodiment, a Clostridial botulinum
serotype A NTNH comprising a .beta.-trefoil domain with enhanced
binding activity comprises a polypeptide having, e.g., at least
one, two, three, four, five, six, seven, eight, nine, 10 or 20
contiguous amino acid deletions relative to amino acids 1050-1097,
amino acids 1111-1138, or amino acids 1149-1194 of SEQ ID NO: 25.
In still other aspects of this embodiment, a Clostridial botulinum
serotype A NTNH comprising a .beta.-trefoil domain with enhanced
binding activity comprises a polypeptide having, e.g., at most one,
two, three, four, five, six, seven, eight, nine, 10 or 20
contiguous amino acid additions relative to amino acids 1050-1097,
amino acids 1111-1138, or amino acids 1149-1194 of SEQ ID NO: 25.
In other aspects of this embodiment, a Clostridial botulinum
serotype A NTNH comprising a .beta.-trefoil domain with enhanced
binding activity comprises a polypeptide having, e.g., at least
one, two, three, four, five, six, seven, eight, nine, 10 or 20
contiguous amino acid additions relative to amino acids 1050-1097,
amino acids 1111-1138, or amino acids 1149-1194 of SEQ ID NO:
25.
[0522] In another embodiment, a .beta.-trefoil domain derived from
a Clostridial botulinum serotype A NTNH comprises a .beta.4/.beta.5
hairpin turn of a .beta.-trefoil domain of a Clostridial botulinum
serotype A NTNH or a 138/139 hairpin turn of a .beta.-trefoil
domain of a Clostridial botulinum serotype A NTNH of SEQ ID NO: 25.
In another aspect of this embodiment, a .beta.-trefoil domain
derived from a Clostridial botulinum serotype A NTNH comprises
amino acids 1098-1110 or amino acids 1139-1148 of SEQ ID NO:
25.
[0523] In another embodiment, a .beta.-trefoil domain with enhanced
binding activity derived from a Clostridial botulinum serotype A
NTNH comprises a modified .beta.4/.beta.5 hairpin turn of a
.beta.-trefoil domain of a Clostridial botulinum serotype A NTNH or
a modified .beta.8/.beta.9 hairpin turn of a .beta.-trefoil domain
of a Clostridial botulinum serotype A NTNH of SEQ ID NO: 25. In
another aspect of this embodiment, a .beta.-trefoil domain with
enhanced binding activity derived from a Clostridial botulinum
serotype A NTNH comprises a modification of amino acids 1098-1110
or amino acids 1139-1148 of SEQ ID NO: 25.
[0524] In other aspects of this embodiment, a .beta.-trefoil domain
with enhanced binding activity derived from a Clostridial botulinum
serotype A NTNH comprises a polypeptide having, e.g., at least 70%
amino acid identity with amino acids 1098-1110 or amino acids
1139-1148 of SEQ ID NO: 25, at least 75% amino acid identity with
amino acids 1098-1110 or amino acids 1139-1148 of SEQ ID NO: 25, at
least 80% amino acid identity with amino acids 1098-1110 or amino
acids 1139-1148 of SEQ ID NO: 25, at least 85% amino acid identity
with amino acids 1098-1110 or amino acids 1139-1148 of SEQ ID NO:
25, at least 90% amino acid identity with amino acids 1098-1110 or
amino acids 1139-1148 of SEQ ID NO: 25 or at least 95% amino acid
identity with amino acids 1098-1110 or amino acids 1139-1148 of SEQ
ID NO: 25. In yet other aspects of this embodiment, a
.beta.-trefoil domain with enhanced binding activity derived from a
Clostridial botulinum serotype A NTNH comprises a polypeptide
having, e.g., at most 70% amino acid identity with amino acids
1098-1110 or amino acids 1139-1148 of SEQ ID NO: 25, at most 75%
amino acid identity with amino acids 1098-1110 or amino acids
1139-1148 of SEQ ID NO: 25, at most 80% amino acid identity with
amino acids 1098-1110 or amino acids 1139-1148 of SEQ ID NO: 25, at
most 85% amino acid identity with amino acids 1098-1110 or amino
acids 1139-1148 of SEQ ID NO: 25, at most 90% amino acid identity
with amino acids 1098-1110 or amino acids 1139-1148 of SEQ ID NO:
25 or at most 95% amino acid identity with amino acids 1098-1110 or
amino acids 1139-1148 of SEQ ID NO: 25.
[0525] In other aspects of this embodiment, a .beta.-trefoil domain
with enhanced binding activity derived from a Clostridial botulinum
serotype A NTNH comprises a polypeptide having, e.g., at most one,
two, three or four non-contiguous amino acid substitutions relative
to amino acids 1098-1110 or amino acids 1139-1148 of SEQ ID NO: 25.
In other aspects of this embodiment, a .beta.-trefoil domain with
enhanced binding activity derived from a Clostridial botulinum
serotype A NTNH comprises a polypeptide having, e.g., at least one,
two, three or four non-contiguous amino acid substitutions relative
to amino acids 1098-1110 or amino acids 1139-1148 of SEQ ID NO: 25.
In other aspects of this embodiment, a non-contiguous amino acid
substitution of any amino acid from amino acids 1098-1110 or amino
acids 1139-1148 of SEQ ID NO: 25 can be replaced with glycine. In
other aspects of this embodiment, a non-contiguous amino acid
substitution of any hydrophobic amino acid from amino acids
1098-1110 or amino acids 1139-1148 of SEQ ID NO: 25 can be replaced
with phenylalanine. In yet other aspects of this embodiment, a
.beta.-trefoil domain with enhanced binding activity derived from a
Clostridial botulinum serotype A NTNH comprises a polypeptide
having, e.g., at most one, two, three or four non-contiguous amino
acid deletions relative to amino acids 1098-1110 or amino acids
1139-1148 of SEQ ID NO: 25. In other aspects of this embodiment, a
.beta.-trefoil domain with enhanced binding activity derived from a
Clostridial botulinum serotype A NTNH comprises a polypeptide
having, e.g., at least one, two, three or four non-contiguous amino
acid deletions relative to amino acids 1098-1110 or amino acids
1139-1148 of SEQ ID NO: 25. In still other aspects of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a Clostridial botulinum serotype A NTNH comprises a
polypeptide having, e.g., at most one, two, three or four
non-contiguous amino acid additions relative to amino acids
1098-1110 or amino acids 1139-1148 of SEQ ID NO: 25. In other
aspects of this embodiment, a .beta.-trefoil domain with enhanced
binding activity derived from a Clostridial botulinum serotype A
NTNH comprises a polypeptide having, e.g., at least one, two, three
or four non-contiguous amino acid additions relative to amino acids
1098-1110 or amino acids 1139-1148 of SEQ ID NO: 25.
[0526] In other aspects of this embodiment, a .beta.-trefoil domain
with enhanced binding activity derived from a Clostridial botulinum
serotype A NTNH comprises a polypeptide having, e.g., at most one,
two, three or four contiguous amino acid substitutions relative to
amino acids 1098-1110 or amino acids 1139-1148 of SEQ ID NO: 25. In
other aspects of this embodiment, a .beta.-trefoil domain with
enhanced binding activity derived from a Clostridial botulinum
serotype A NTNH comprises a polypeptide having, e.g., at least one,
two, three or four contiguous amino acid substitutions relative to
amino acids 1098-1110 or amino acids 1139-1148 of SEQ ID NO: 25. In
other aspects of this embodiment, contiguous amino acid
substitutions of amino acids from amino acids 1098-1110 or amino
acids 1139-1148 of SEQ ID NO: 25 can be replaced with glycine. In
other aspects of this embodiment, contiguous amino acid
substitutions of hydrophobic amino acids from amino acids 1098-1110
or amino acids 1139-1148 of SEQ ID NO: 25 can be replaced with
phenylalanine. In yet other aspects of this embodiment, a
.beta.-trefoil domain with enhanced binding activity derived from a
Clostridial botulinum serotype A NTNH comprises a polypeptide
having, e.g., at most one, two, three or four contiguous amino acid
deletions relative to amino acids 1098-1110 or amino acids
1139-1148 of SEQ ID NO: 25. In other aspects of this embodiment, a
.beta.-trefoil domain with enhanced binding activity derived from a
Clostridial botulinum serotype A NTNH comprises a polypeptide
having, e.g., at least one, two, three or four contiguous amino
acid deletions relative to amino acids 1098-1110 or amino acids
1139-1148 of SEQ ID NO: 25. In still other aspects of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a Clostridial botulinum serotype A NTNH comprises a
polypeptide having, e.g., at most one, two, three or four
contiguous amino acid additions relative to amino acids 1098-1110
or amino acids 1139-1148 of SEQ ID NO: 25. In other aspects of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a Clostridial botulinum serotype A NTNH comprises a
polypeptide having, e.g., at least one, two, three or four
contiguous amino acid additions relative to amino acids 1098-1110
or amino acids 1139-1148 of SEQ ID NO: 25.
[0527] In another embodiment, a .beta.-trefoil domain derived from
a Clostridial botulinum serotype B NTNH comprises a .beta.-trefoil
domain derived from a Clostridial botulinum serotype B NTNH of SEQ
ID NO: 26. In another embodiment, a .beta.-trefoil domain derived
from a Clostridial botulinum serotype B NTNH comprises amino acids
1049-1198 of SEQ ID NO: 26. In another aspect of this embodiment, a
.beta.-trefoil domain derived from a Clostridial botulinum serotype
B NTNH comprises a .alpha.-fold motif of a .beta.-trefoil domain of
a Clostridial botulinum serotype B NTNH, a .beta.-fold motif of a
.beta.-trefoil domain of a Clostridial botulinum serotype B NTNH or
a .gamma.-fold motif of a .beta.-trefoil domain of a Clostridial
botulinum serotype B NTNH of SEQ ID NO: 26. In another aspect of
this embodiment, a .beta.-trefoil domain derived from a Clostridial
botulinum serotype B NTNH comprises amino acids 1049-1096, amino
acids 1110-1138, or amino acids 1148-1198 of SEQ ID NO: 26.
[0528] In another embodiment, a .beta.-trefoil domain with enhanced
binding activity derived from a Clostridial botulinum serotype B
NTNH comprises a .beta.-trefoil domain with enhanced binding
activity derived from a Clostridial botulinum serotype B NTNH of
SEQ ID NO: 26. In another embodiment, a .beta.-trefoil domain with
enhanced binding activity derived from a Clostridial botulinum
serotype B NTNH comprises amino acids 1049-1198 of SEQ ID NO: 26.
In another aspect of this embodiment, a .beta.-trefoil domain with
enhanced binding activity derived from a Clostridial botulinum
serotype B NTNH comprises a modified .alpha.-fold motif of a
.beta.-trefoil domain of a Clostridial botulinum serotype B NTNH, a
modified .beta.-fold motif of a .beta.-trefoil domain of a
Clostridial botulinum serotype B NTNH or a modified .gamma.-fold
motif of a 13-trefoil domain of a Clostridial botulinum serotype B
NTNH of SEQ ID NO: 26. In another aspect of this embodiment, a
.beta.-trefoil domain with enhanced binding activity derived from a
Clostridial botulinum serotype B NTNH comprises amino acids
1049-1096, amino acids 1110-1138, or amino acids 1148-1198 of SEQ
ID NO: 26.
[0529] In other aspects of this embodiment, a Clostridial botulinum
serotype B NTNH comprising a .beta.-trefoil domain with enhanced
binding activity comprises a polypeptide having, e.g., at least 70%
amino acid identity with amino acids 1049-1096, amino acids
1110-1138, or amino acids 1148-1198 of SEQ ID NO: 26, at least 75%
amino acid identity with amino acids 1049-1096, amino acids
1110-1138, or amino acids 1148-1198 of SEQ ID NO: 26, at least 80%
amino acid identity with amino acids 1049-1096, amino acids
1110-1138, or amino acids 1148-1198 of SEQ ID NO: 26, at least 85%
amino acid identity with amino acids 1049-1096, amino acids
1110-1138, or amino acids 1148-1198 of SEQ ID NO: 26, at least 90%
amino acid identity with amino acids 1049-1096, amino acids
1110-1138, or amino acids 1148-1198 of SEQ ID NO: 26 or at least
95% amino acid identity with amino acids 1049-1096, amino acids
1110-1138, or amino acids 1148-1198 of SEQ ID NO: 26. In yet other
aspects of this embodiment, a Clostridial botulinum serotype B NTNH
comprising a .beta.-trefoil domain with enhanced binding activity
comprises a polypeptide having, e.g., at most 70% amino acid
identity with amino acids 1049-1096, amino acids 1110-1138, or
amino acids 1148-1198 of SEQ ID NO: 26, at most 75% amino acid
identity with amino acids 1049-1096, amino acids 1110-1138, or
amino acids 1148-1198 of SEQ ID NO: 26, at most 80% amino acid
identity with amino acids 1049-1096, amino acids 1110-1138, or
amino acids 1148-1198 of SEQ ID NO: 26, at most 85% amino acid
identity with amino acids 1049-1096, amino acids 1110-1138, or
amino acids 1148-1198 of SEQ ID NO: 26, at most 90% amino acid
identity with amino acids 1049-1096, amino acids 1110-1138, or
amino acids 1148-1198 of SEQ ID NO: 26 or at most 95% amino acid
identity with amino acids 1049-1096, amino acids 1110-1138, or
amino acids 1148-1198 of SEQ ID NO: 26.
[0530] In other aspects of this embodiment, a Clostridial botulinum
serotype B NTNH comprising a 11-trefoil domain with enhanced
binding activity comprises a polypeptide having, e.g., at most one,
two, three, four, five, six, seven, eight, nine, 10 or 20
non-contiguous amino acid substitutions relative to amino acids
1049-1096, amino acids 1110-1138, or amino acids 1148-1198 of SEQ
ID NO: 26. In other aspects of this embodiment, a Clostridial
botulinum serotype B NTNH comprising a .beta.-trefoil domain with
enhanced binding activity comprises a polypeptide having, e.g., at
least one, two, three, four, five, six, seven, eight, nine, 10 or
20 non-contiguous amino acid substitutions relative to amino acids
1049-1096, amino acids 1110-1138, or amino acids 1148-1198 of SEQ
ID NO: 26. In yet other aspects of this embodiment, a Clostridial
botulinum serotype B NTNH comprising a .beta.-trefoil domain with
enhanced binding activity comprises a polypeptide having, e.g., at
most one, two, three, four, five, six, seven, eight, nine, 10 or 20
non-contiguous amino acid deletions relative to amino acids
1049-1096, amino acids 1110-1138, or amino acids 1148-1198 of SEQ
ID NO: 26. In other aspects of this embodiment, a Clostridial
botulinum serotype B NTNH comprising a .beta.-trefoil domain with
enhanced binding activity comprises a polypeptide having, e.g., at
least one, two, three, four, five, six, seven, eight, nine, 10 or
20 non-contiguous amino acid deletions relative to amino acids
1049-1096, amino acids 1110-1138, or amino acids 1148-1198 of SEQ
ID NO: 26. In still other aspects of this embodiment, a Clostridial
botulinum serotype B NTNH comprising a .beta.-trefoil domain with
enhanced binding activity comprises a polypeptide having, e.g., at
most one, two, three, four, five, six, seven, eight, nine, 10 or 20
non-contiguous amino acid additions relative to amino acids
1049-1096, amino acids 1110-1138, or amino acids 1148-1198 of SEQ
ID NO: 26. In other aspects of this embodiment, a Clostridial
botulinum serotype B NTNH comprising a .beta.-trefoil domain with
enhanced binding activity comprises a polypeptide having, e.g., at
least one, two, three, four, five, six, seven, eight, nine, 10 or
20 non-contiguous amino acid additions relative to amino acids
1049-1096, amino acids 1110-1138, or amino acids 1148-1198 of SEQ
ID NO: 26.
[0531] In other aspects of this embodiment, a Clostridial botulinum
serotype B NTNH comprising a .beta.-trefoil domain with enhanced
binding activity comprises a polypeptide having, e.g., at most one,
two, three, four, five, six, seven, eight, nine, 10 or 20
contiguous amino acid substitutions relative to amino acids
1049-1096, amino acids 1110-1138, or amino acids 1148-1198 of SEQ
ID NO: 26. In other aspects of this embodiment, a Clostridial
botulinum serotype B NTNH comprising a .beta.-trefoil domain with
enhanced binding activity comprises a polypeptide having, e.g., at
least one, two, three, four, five, six, seven, eight, nine, 10 or
20 contiguous amino acid substitutions relative to amino acids
1049-1096, amino acids 1110-1138, or amino acids 1148-1198 of SEQ
ID NO: 26. In yet other aspects of this embodiment, a Clostridial
botulinum serotype B NTNH comprising a .beta.-trefoil domain with
enhanced binding activity comprises a polypeptide having, e.g., at
most one, two, three, four, five, six, seven, eight, nine, 10 or 20
contiguous amino acid deletions relative to amino acids 1049-1096,
amino acids 1110-1138, or amino acids 1148-1198 of SEQ ID NO: 26.
In other aspects of this embodiment, a Clostridial botulinum
serotype B NTNH comprising a .beta.-trefoil domain with enhanced
binding activity comprises a polypeptide having, e.g., at least
one, two, three, four, five, six, seven, eight, nine, 10 or 20
contiguous amino acid deletions relative to amino acids 1049-1096,
amino acids 1110-1138, or amino acids 1148-1198 of SEQ ID NO: 26.
In still other aspects of this embodiment, a Clostridial botulinum
serotype B NTNH comprising a .beta.-trefoil domain with enhanced
binding activity comprises a polypeptide having, e.g., at most one,
two, three, four, five, six, seven, eight, nine, 10 or 20
contiguous amino acid additions relative to amino acids 1049-1096,
amino acids 1110-1138, or amino acids 1148-1198 of SEQ ID NO: 26.
In other aspects of this embodiment, a Clostridial botulinum
serotype B NTNH comprising a .beta.-trefoil domain with enhanced
binding activity comprises a polypeptide having, e.g., at least
one, two, three, four, five, six, seven, eight, nine, 10 or 20
contiguous amino acid additions relative to amino acids 1049-1096,
amino acids 1110-1138, or amino acids 1148-1198 of SEQ ID NO:
26.
[0532] In another embodiment, a .beta.-trefoil domain derived from
a Clostridial botulinum serotype B NTNH comprises a .beta.4/.beta.5
hairpin turn of a .beta.-trefoil domain of a Clostridial botulinum
serotype B NTNH or a 138/139 hairpin turn of a .beta.-trefoil
domain of a Clostridial botulinum serotype B NTNH of SEQ ID NO: 26.
In another aspect of this embodiment, a .beta.-trefoil domain
derived from a Clostridial botulinum serotype B NTNH comprises
amino acids 1097-1109 or amino acids 1139-1147 of SEQ ID NO:
26.
[0533] In another embodiment, a .beta.-trefoil domain with enhanced
binding activity derived from a Clostridial botulinum serotype B
NTNH comprises a modified .beta.4/.beta.5 hairpin turn of a
.beta.-trefoil domain of a Clostridial botulinum serotype B NTNH or
a modified .beta.8/.beta.9 hairpin turn of a .beta.-trefoil domain
of a Clostridial botulinum serotype B NTNH of SEQ ID NO: 26. In
another aspect of this embodiment, a .beta.-trefoil domain with
enhanced binding activity derived from a Clostridial botulinum
serotype B NTNH comprises a modification of amino acids 1097-1109
or amino acids 1139-1147 of SEQ ID NO: 26.
[0534] In other aspects of this embodiment, a .beta.-trefoil domain
with enhanced binding activity derived from a Clostridial botulinum
serotype B NTNH comprises a polypeptide having, e.g., at least 70%
amino acid identity with amino acids 1097-1109 or amino acids
1139-1147 of SEQ ID NO: 26, at least 75% amino acid identity with
amino acids 1097-1109 or amino acids 1139-1147 of SEQ ID NO: 26, at
least 80% amino acid identity with amino acids 1097-1109 or amino
acids 1139-1147 of SEQ ID NO: 26, at least 85% amino acid identity
with amino acids 1097-1109 or amino acids 1139-1147 of SEQ ID NO:
26, at least 90% amino acid identity with amino acids 1097-1109 or
amino acids 1139-1147 of SEQ ID NO: 26 or at least 95% amino acid
identity with amino acids 1097-1109 or amino acids 1139-1147 of SEQ
ID NO: 26. In yet other aspects of this embodiment, a
.beta.-trefoil domain with enhanced binding activity derived from a
Clostridial botulinum serotype B NTNH comprises a polypeptide
having, e.g., at most 70% amino acid identity with amino acids
1097-1109 or amino acids 1139-1147 of SEQ ID NO: 26, at most 75%
amino acid identity with amino acids 1097-1109 or amino acids
1139-1147 of SEQ ID NO: 26, at most 80% amino acid identity with
amino acids 1097-1109 or amino acids 1139-1147 of SEQ ID NO: 26, at
most 85% amino acid identity with amino acids 1097-1109 or amino
acids 1139-1147 of SEQ ID NO: 26, at most 90% amino acid identity
with amino acids 1097-1109 or amino acids 1139-1147 of SEQ ID NO:
26 or at most 95% amino acid identity with amino acids 1097-1109 or
amino acids 1139-1147 of SEQ ID NO: 26.
[0535] In other aspects of this embodiment, a .beta.-trefoil domain
with enhanced binding activity derived from a Clostridial botulinum
serotype B NTNH comprises a polypeptide having, e.g., at most one,
two, three or four non-contiguous amino acid substitutions relative
to amino acids 1097-1109 or amino acids 1139-1147 of SEQ ID NO: 26.
In other aspects of this embodiment, a .beta.-trefoil domain with
enhanced binding activity derived from a Clostridial botulinum
serotype B NTNH comprises a polypeptide having, e.g., at least one,
two, three or four non-contiguous amino acid substitutions relative
to amino acids 1097-1109 or amino acids 1139-1147 of SEQ ID NO: 26.
In other aspects of this embodiment, a non-contiguous amino acid
substitution of any amino acid from amino acids 1097-1109 or amino
acids 1139-1147 of SEQ ID NO: 26 can be replaced with glycine. In
other aspects of this embodiment, a non-contiguous amino acid
substitution of any hydrophobic amino acid from amino acids
1097-1109 or amino acids 1139-1147 of SEQ ID NO: 26 can be replaced
with phenylalanine. In yet other aspects of this embodiment, a
.beta.-trefoil domain with enhanced binding activity derived from a
Clostridial botulinum serotype B NTNH comprises a polypeptide
having, e.g., at most one, two, three or four non-contiguous amino
acid deletions relative to amino acids 1097-1109 or amino acids
1139-1147 of SEQ ID NO: 26. In other aspects of this embodiment, a
.beta.-trefoil domain with enhanced binding activity derived from a
Clostridial botulinum serotype B NTNH comprises a polypeptide
having, e.g., at least one, two, three or four non-contiguous amino
acid deletions relative to amino acids 1097-1109 or amino acids
1139-1147 of SEQ ID NO: 26. In still other aspects of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a Clostridial botulinum serotype B NTNH comprises a
polypeptide having, e.g., at most one, two, three or four
non-contiguous amino acid additions relative to amino acids
1097-1109 or amino acids 1139-1147 of SEQ ID NO: 26. In other
aspects of this embodiment, a .beta.-trefoil domain with enhanced
binding activity derived from a Clostridial botulinum serotype B
NTNH comprises a polypeptide having, e.g., at least one, two, three
or four non-contiguous amino acid additions relative to amino acids
1097-1109 or amino acids 1139-1147 of SEQ ID NO: 26.
[0536] In other aspects of this embodiment, a .beta.-trefoil domain
with enhanced binding activity derived from a Clostridial botulinum
serotype B NTNH comprises a polypeptide having, e.g., at most one,
two, three or four contiguous amino acid substitutions relative to
amino acids 1097-1109 or amino acids 1139-1147 of SEQ ID NO: 26. In
other aspects of this embodiment, a .beta.-trefoil domain with
enhanced binding activity derived from a Clostridial botulinum
serotype B NTNH comprises a polypeptide having, e.g., at least one,
two, three or four contiguous amino acid substitutions relative to
amino acids 1097-1109 or amino acids 1139-1147 of SEQ ID NO: 26. In
other aspects of this embodiment, contiguous amino acid
substitutions of amino acids from amino acids 1097-1109 or amino
acids 1139-1147 of SEQ ID NO: 26 can be replaced with glycine. In
other aspects of this embodiment, contiguous amino acid
substitutions of hydrophobic amino acids from amino acids 1097-1109
or amino acids 1139-1147 of SEQ ID NO: 26 can be replaced with
phenylalanine. In yet other aspects of this embodiment, a
.beta.-trefoil domain with enhanced binding activity derived from a
Clostridial botulinum serotype B NTNH comprises a polypeptide
having, e.g., at most one, two, three or four contiguous amino acid
deletions relative to amino acids 1097-1109 or amino acids
1139-1147 of SEQ ID NO: 26. In other aspects of this embodiment, a
.beta.-trefoil domain with enhanced binding activity derived from a
Clostridial botulinum serotype B NTNH comprises a polypeptide
having, e.g., at least one, two, three or four contiguous amino
acid deletions relative to amino acids 1097-1109 or amino acids
1139-1147 of SEQ ID NO: 26. In still other aspects of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a Clostridial botulinum serotype B NTNH comprises a
polypeptide having, e.g., at most one, two, three or four
contiguous amino acid additions relative to amino acids 1097-1109
or amino acids 1139-1147 of SEQ ID NO: 26. In other aspects of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a Clostridial botulinum serotype B NTNH comprises a
polypeptide having, e.g., at least one, two, three or four
contiguous amino acid additions relative to amino acids 1097-1109
or amino acids 1139-1147 of SEQ ID NO: 26.
[0537] In another embodiment, a .beta.-trefoil domain derived from
a Clostridial botulinum serotype Cl NTNH comprises a .beta.-trefoil
domain derived from a Clostridial botulinum serotype C1 NTNH of SEQ
ID NO: 27. In another embodiment, a .beta.-trefoil domain derived
from a Clostridial botulinum serotype Cl NTNH comprises amino acids
1049-1197 of SEQ ID NO: 27. In another aspect of this embodiment, a
.beta.-trefoil domain derived from a Clostridial botulinum serotype
C1 NTNH comprises a .alpha.-fold motif of a 13-trefoil domain of a
Clostridial botulinum serotype C1 NTNH, a .beta.-fold motif of a
.beta.-trefoil domain of a Clostridial botulinum serotype C1 NTNH
or a .gamma.-fold motif of a .beta.-trefoil domain of a Clostridial
botulinum serotype C1 NTNH of SEQ ID NO: 27. In another aspect of
this embodiment, a .beta.-trefoil domain derived from a Clostridial
botulinum serotype C1 NTNH comprises amino acids 1049-1096, amino
acids 1110-1138, or amino acids 1148-1197 of SEQ ID NO: 27.
[0538] In another embodiment, a .beta.-trefoil domain with enhanced
binding activity derived from a Clostridial botulinum serotype C1
NTNH comprises a .beta.-trefoil domain with enhanced binding
activity derived from a Clostridial botulinum serotype C1 NTNH of
SEQ ID NO: 27. In another embodiment, a .beta.-trefoil domain with
enhanced binding activity derived from a Clostridial botulinum
serotype C1 NTNH comprises amino acids 1049-1197 of SEQ ID NO: 27.
In another aspect of this embodiment, a .beta.-trefoil domain with
enhanced binding activity derived from a Clostridial botulinum
serotype C1 NTNH comprises a modified .alpha.-fold motif of a
.beta.-trefoil domain of a Clostridial botulinum serotype C1 NTNH,
a modified .beta.-fold motif of a .beta.-trefoil domain of a
Clostridial botulinum serotype C1 NTNH or a modified .gamma.-fold
motif of a .beta.-trefoil domain of a Clostridial botulinum
serotype C1 NTNH of SEQ ID NO: 27. In another aspect of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a Clostridial botulinum serotype C1 NTNH comprises
amino acids 1049-1096, amino acids 1110-1138, or amino acids
1148-1197 of SEQ ID NO: 27.
[0539] In other aspects of this embodiment, a Clostridial botulinum
serotype C1 NTNH comprising a .beta.-trefoil domain with enhanced
binding activity comprises a polypeptide having, e.g., at least 70%
amino acid identity with amino acids 1049-1096, amino acids
1110-1138, or amino acids 1148-1197 of SEQ ID NO: 27, at least 75%
amino acid identity with amino acids 1049-1096, amino acids
1110-1138, or amino acids 1148-1197 of SEQ ID NO: 27, at least 80%
amino acid identity with amino acids 1049-1096, amino acids
1110-1138, or amino acids 1148-1197 of SEQ ID NO: 27, at least 85%
amino acid identity with amino acids 1049-1096, amino acids
1110-1138, or amino acids 1148-1197 of SEQ ID NO: 27, at least 90%
amino acid identity with amino acids 1049-1096, amino acids
1110-1138, or amino acids 1148-1197 of SEQ ID NO: 27 or at least
95% amino acid identity with amino acids 1049-1096, amino acids
1110-1138, or amino acids 1148-1197 of SEQ ID NO: 27. In yet other
aspects of this embodiment, a Clostridial botulinum serotype C1
NTNH comprising a .beta.-trefoil domain with enhanced binding
activity comprises a polypeptide having, e.g., at most 70% amino
acid identity with amino acids 1049-1096, amino acids 1110-1138, or
amino acids 1148-1197 of SEQ ID NO: 27, at most 75% amino acid
identity with amino acids 1049-1096, amino acids 1110-1138, or
amino acids 1148-1197 of SEQ ID NO: 27, at most 80% amino acid
identity with amino acids 1049-1096, amino acids 1110-1138, or
amino acids 1148-1197 of SEQ ID NO: 27, at most 85% amino acid
identity with amino acids 1049-1096, amino acids 1110-1138, or
amino acids 1148-1197 of SEQ ID NO: 27, at most 90% amino acid
identity with amino acids 1049-1096, amino acids 1110-1138, or
amino acids 1148-1197 of SEQ ID NO: 27 or at most 95% amino acid
identity with amino acids 1049-1096, amino acids 1110-1138, or
amino acids 1148-1197 of SEQ ID NO: 27.
[0540] In other aspects of this embodiment, a Clostridial botulinum
serotype C1 NTNH comprising a .beta.-trefoil domain with enhanced
binding activity comprises a polypeptide having, e.g., at most one,
two, three, four, five, six, seven, eight, nine, 10 or 20
non-contiguous amino acid substitutions relative to amino acids
1049-1096, amino acids 1110-1138, or amino acids 1148-1197 of SEQ
ID NO: 27. In other aspects of this embodiment, a Clostridial
botulinum serotype C1 NTNH comprising a .beta.-trefoil domain with
enhanced binding activity comprises a polypeptide having, e.g., at
least one, two, three, four, five, six, seven, eight, nine, 10 or
20 non-contiguous amino acid substitutions relative to amino acids
1049-1096, amino acids 1110-1138, or amino acids 1148-1197 of SEQ
ID NO: 27. In yet other aspects of this embodiment, a Clostridial
botulinum serotype C1 NTNH comprising a .beta.-trefoil domain with
enhanced binding activity comprises a polypeptide having, e.g., at
most one, two, three, four, five, six, seven, eight, nine, 10 or 20
non-contiguous amino acid deletions relative to amino acids
1049-1096, amino acids 1110-1138, or amino acids 1148-1197 of SEQ
ID NO: 27. In other aspects of this embodiment, a Clostridial
botulinum serotype C1 NTNH comprising a .beta.-trefoil domain with
enhanced binding activity comprises a polypeptide having, e.g., at
least one, two, three, four, five, six, seven, eight, nine, 10 or
20 non-contiguous amino acid deletions relative to amino acids
1049-1096, amino acids 1110-1138, or amino acids 1148-1197 of SEQ
ID NO: 27. In still other aspects of this embodiment, a Clostridial
botulinum serotype C1 NTNH comprising a .beta.-trefoil domain with
enhanced binding activity comprises a polypeptide having, e.g., at
most one, two, three, four, five, six, seven, eight, nine, 10 or 20
non-contiguous amino acid additions relative to amino acids
1049-1096, amino acids 1110-1138, or amino acids 1148-1197 of SEQ
ID NO: 27. In other aspects of this embodiment, a Clostridial
botulinum serotype C1 NTNH comprising a .beta.-trefoil domain with
enhanced binding activity comprises a polypeptide having, e.g., at
least one, two, three, four, five, six, seven, eight, nine, 10 or
20 non-contiguous amino acid additions relative to amino acids
1049-1096, amino acids 1110-1138, or amino acids 1148-1197 of SEQ
ID NO: 27.
[0541] In other aspects of this embodiment, a Clostridial botulinum
serotype C1 NTNH comprising a .beta.-trefoil domain with enhanced
binding activity comprises a polypeptide having, e.g., at most one,
two, three, four, five, six, seven, eight, nine, 10 or 20
contiguous amino acid substitutions relative to amino acids
1049-1096, amino acids 1110-1138, or amino acids 1148-1197 of SEQ
ID NO: 27. In other aspects of this embodiment, a Clostridial
botulinum serotype C1 NTNH comprising a .beta.-trefoil domain with
enhanced binding activity comprises a polypeptide having, e.g., at
least one, two, three, four, five, six, seven, eight, nine, 10 or
20 contiguous amino acid substitutions relative to amino acids
1049-1096, amino acids 1110-1138, or amino acids 1148-1197 of SEQ
ID NO: 27. In yet other aspects of this embodiment, a Clostridial
botulinum serotype C1 NTNH comprising a .beta.-trefoil domain with
enhanced binding activity comprises a polypeptide having, e.g., at
most one, two, three, four, five, six, seven, eight, nine, 10 or 20
contiguous amino acid deletions relative to amino acids 1049-1096,
amino acids 1110-1138, or amino acids 1148-1197 of SEQ ID NO: 27.
In other aspects of this embodiment, a Clostridial botulinum
serotype C1 NTNH comprising a .beta.-trefoil domain with enhanced
binding activity comprises a polypeptide having, e.g., at least
one, two, three, four, five, six, seven, eight, nine, 10 or 20
contiguous amino acid deletions relative to amino acids 1049-1096,
amino acids 1110-1138, or amino acids 1148-1197 of SEQ ID NO: 27.
In still other aspects of this embodiment, a Clostridial botulinum
serotype C1 NTNH comprising a .beta.-trefoil domain with enhanced
binding activity comprises a polypeptide having, e.g., at most one,
two, three, four, five, six, seven, eight, nine, 10 or 20
contiguous amino acid additions relative to amino acids 1049-1096,
amino acids 1110-1138, or amino acids 1148-1197 of SEQ ID NO: 27.
In other aspects of this embodiment, a Clostridial botulinum
serotype C1 NTNH comprising a .beta.-trefoil domain with enhanced
binding activity comprises a polypeptide having, e.g., at least
one, two, three, four, five, six, seven, eight, nine, 10 or 20
contiguous amino acid additions relative to amino acids 1049-1096,
amino acids 1110-1138, or amino acids 1148-1197 of SEQ ID NO:
27.
[0542] In another embodiment, a .beta.-trefoil domain derived from
a Clostridial botulinum serotype Cl NTNH comprises a
.beta.4/.beta.5 hairpin turn of a .beta.-trefoil domain of a
Clostridial botulinum serotype C1 NTNH or a .beta.8/.beta.9 hairpin
turn of a .beta.-trefoil domain of a Clostridial botulinum serotype
C1 NTNH of SEQ ID NO: 27. In another aspect of this embodiment, a
.beta.-trefoil domain derived from a Clostridial botulinum serotype
C1 NTNH comprises amino acids 1097-1109 or amino acids 1139-1147 of
SEQ ID NO: 27.
[0543] In another embodiment, a .beta.-trefoil domain with enhanced
binding activity derived from a Clostridial botulinum serotype C1
NTNH comprises a modified .beta.4/.beta.5 hairpin turn of a
.beta.-trefoil domain of a Clostridial botulinum serotype C1 NTNH
or a modified .beta.8/.beta.9 hairpin turn of a .beta.-trefoil
domain of a Clostridial botulinum serotype C1 NTNH of SEQ ID NO:
27. In another aspect of this embodiment, a .beta.-trefoil domain
with enhanced binding activity derived from a Clostridial botulinum
serotype C1 NTNH comprises a modification of amino acids 1097-1109
or amino acids 1139-1147 of SEQ ID NO: 27.
[0544] In other aspects of this embodiment, a .beta.-trefoil domain
with enhanced binding activity derived from a Clostridial botulinum
serotype C1 NTNH comprises a polypeptide having, e.g., at least 70%
amino acid identity with amino acids 1097-1109 or amino acids
1139-1147 of SEQ ID NO: 27, at least 75% amino acid identity with
amino acids 1097-1109 or amino acids 1139-1147 of SEQ ID NO: 27, at
least 80% amino acid identity with amino acids 1097-1109 or amino
acids 1139-1147 of SEQ ID NO: 27, at least 85% amino acid identity
with amino acids 1097-1109 or amino acids 1139-1147 of SEQ ID NO:
27, at least 90% amino acid identity with amino acids 1097-1109 or
amino acids 1139-1147 of SEQ ID NO: 27 or at least 95% amino acid
identity with amino acids 1097-1109 or amino acids 1139-1147 of SEQ
ID NO: 27. In yet other aspects of this embodiment, a
.beta.-trefoil domain with enhanced binding activity derived from a
Clostridial botulinum serotype C1 NTNH comprises a polypeptide
having, e.g., at most 70% amino acid identity with amino acids
1097-1109 or amino acids 1139-1147 of SEQ ID NO: 27, at most 75%
amino acid identity with amino acids 1097-1109 or amino acids
1139-1147 of SEQ ID NO: 27, at most 80% amino acid identity with
amino acids 1097-1109 or amino acids 1139-1147 of SEQ ID NO: 27, at
most 85% amino acid identity with amino acids 1097-1109 or amino
acids 1139-1147 of SEQ ID NO: 27, at most 90% amino acid identity
with amino acids 1097-1109 or amino acids 1139-1147 of SEQ ID NO:
27 or at most 95% amino acid identity with amino acids 1097-1109 or
amino acids 1139-1147 of SEQ ID NO: 27.
[0545] In other aspects of this embodiment, a .beta.-trefoil domain
with enhanced binding activity derived from a Clostridial botulinum
serotype C1 NTNH comprises a polypeptide having, e.g., at most one,
two, three or four non-contiguous amino acid substitutions relative
to amino acids 1097-1109 or amino acids 1139-1147 of SEQ ID NO: 27.
In other aspects of this embodiment, a .beta.-trefoil domain with
enhanced binding activity derived from a Clostridial botulinum
serotype C1 NTNH comprises a polypeptide having, e.g., at least
one, two, three or four non-contiguous amino acid substitutions
relative to amino acids 1097-1109 or amino acids 1139-1147 of SEQ
ID NO: 27. In other aspects of this embodiment, a non-contiguous
amino acid substitution of any amino acid from amino acids
1097-1109 or amino acids 1139-1147 of SEQ ID NO: 27 can be replaced
with glycine. In other aspects of this embodiment, a non-contiguous
amino acid substitution of any hydrophobic amino acid from amino
acids 1097-1109 or amino acids 1139-1147 of SEQ ID NO: 27 can be
replaced with phenylalanine. In yet other aspects of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a Clostridial botulinum serotype C1 NTNH comprises a
polypeptide having, e.g., at most one, two, three or four
non-contiguous amino acid deletions relative to amino acids
1097-1109 or amino acids 1139-1147 of SEQ ID NO: 27. In other
aspects of this embodiment, a .beta.-trefoil domain with enhanced
binding activity derived from a Clostridial botulinum serotype C1
NTNH comprises a polypeptide having, e.g., at least one, two, three
or four non-contiguous amino acid deletions relative to amino acids
1097-1109 or amino acids 1139-1147 of SEQ ID NO: 27. In still other
aspects of this embodiment, a .beta.-trefoil domain with enhanced
binding activity derived from a Clostridial botulinum serotype C1
NTNH comprises a polypeptide having, e.g., at most one, two, three
or four non-contiguous amino acid additions relative to amino acids
1097-1109 or amino acids 1139-1147 of SEQ ID NO: 27. In other
aspects of this embodiment, a .beta.-trefoil domain with enhanced
binding activity derived from a Clostridial botulinum serotype C1
NTNH comprises a polypeptide having, e.g., at least one, two, three
or four non-contiguous amino acid additions relative to amino acids
1097-1109 or amino acids 1139-1147 of SEQ ID NO: 27.
[0546] In other aspects of this embodiment, a .beta.-trefoil domain
with enhanced binding activity derived from a Clostridial botulinum
serotype C1 NTNH comprises a polypeptide having, e.g., at most one,
two, three or four contiguous amino acid substitutions relative to
amino acids 1097-1109 or amino acids 1139-1147 of SEQ ID NO: 27. In
other aspects of this embodiment, a .beta.-trefoil domain with
enhanced binding activity derived from a Clostridial botulinum
serotype C1 NTNH comprises a polypeptide having, e.g., at least
one, two, three or four contiguous amino acid substitutions
relative to amino acids 1097-1109 or amino acids 1139-1147 of SEQ
ID NO: 27. In other aspects of this embodiment, contiguous amino
acid substitutions of amino acids from amino acids 1097-1109 or
amino acids 1139-1147 of SEQ ID NO: 27 can be replaced with
glycine. In other aspects of this embodiment, contiguous amino acid
substitutions of hydrophobic amino acids from amino acids 1097-1109
or amino acids 1139-1147 of SEQ ID NO: 27 can be replaced with
phenylalanine. In yet other aspects of this embodiment, a
.beta.-trefoil domain with enhanced binding activity derived from a
Clostridial botulinum serotype C1 NTNH comprises a polypeptide
having, e.g., at most one, two, three or four contiguous amino acid
deletions relative to amino acids 1097-1109 or amino acids
1139-1147 of SEQ ID NO: 27. In other aspects of this embodiment, a
.beta.-trefoil domain with enhanced binding activity derived from a
Clostridial botulinum serotype C1 NTNH comprises a polypeptide
having, e.g., at least one, two, three or four contiguous amino
acid deletions relative to amino acids 1097-1109 or amino acids
1139-1147 of SEQ ID NO: 27. In still other aspects of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a Clostridial botulinum serotype C1 NTNH comprises a
polypeptide having, e.g., at most one, two, three or four
contiguous amino acid additions relative to amino acids 1097-1109
or amino acids 1139-1147 of SEQ ID NO: 27. In other aspects of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a Clostridial botulinum serotype C1 NTNH comprises a
polypeptide having, e.g., at least one, two, three or four
contiguous amino acid additions relative to amino acids 1097-1109
or amino acids 1139-1147 of SEQ ID NO: 27.
[0547] In another embodiment, a .beta.-trefoil domain derived from
a Clostridial botulinum serotype D NTNH comprises a .beta.-trefoil
domain derived from a Clostridial botulinum serotype D NTNH of SEQ
ID NO: 28. In another embodiment, a .beta.-trefoil domain derived
from a Clostridial botulinum serotype D NTNH comprises amino acids
1049-1197 of SEQ ID NO: 28. In another aspect of this embodiment, a
.beta.-trefoil domain derived from a Clostridial botulinum serotype
D NTNH comprises a .alpha.-fold motif of a .beta.-trefoil domain of
a Clostridial botulinum serotype D NTNH, a .beta.-fold motif of a
.beta.-trefoil domain of a Clostridial botulinum serotype D NTNH or
a .gamma.-fold motif of a .beta.-trefoil domain of a Clostridial
botulinum serotype D NTNH of SEQ ID NO: 28. In another aspect of
this embodiment, a .beta.-trefoil domain derived from a Clostridial
botulinum serotype D NTNH comprises amino acids 1049-1096, amino
acids 1110-1138, or amino acids 1148-1197 of SEQ ID NO: 28.
[0548] In another embodiment, a .beta.-trefoil domain with enhanced
binding activity derived from a Clostridial botulinum serotype D
NTNH comprises a .beta.-trefoil domain with enhanced binding
activity derived from a Clostridial botulinum serotype D NTNH of
SEQ ID NO: 28. In another embodiment, a .beta.-trefoil domain with
enhanced binding activity derived from a Clostridial botulinum
serotype D NTNH comprises amino acids 1049-1197 of SEQ ID NO: 28.
In another aspect of this embodiment, a .beta.-trefoil domain with
enhanced binding activity derived from a Clostridial botulinum
serotype D NTNH comprises a modified .alpha.-fold motif of a
.beta.-trefoil domain of a Clostridial botulinum serotype D NTNH, a
modified .beta.-fold motif of a .beta.-trefoil domain of a
Clostridial botulinum serotype D NTNH or a modified .gamma.-fold
motif of a 13-trefoil domain of a Clostridial botulinum serotype D
NTNH of SEQ ID NO: 28. In another aspect of this embodiment, a
.beta.-trefoil domain with enhanced binding activity derived from a
Clostridial botulinum serotype D NTNH comprises amino acids
1049-1096, amino acids 1110-1138, or amino acids 1148-1197 of SEQ
ID NO: 28.
[0549] In other aspects of this embodiment, a Clostridial botulinum
serotype D NTNH comprising a .beta.-trefoil domain with enhanced
binding activity comprises a polypeptide having, e.g., at least 70%
amino acid identity with amino acids 1049-1096, amino acids
1110-1138, or amino acids 1148-1197 of SEQ ID NO: 28, at least 75%
amino acid identity with amino acids 1049-1096, amino acids
1110-1138, or amino acids 1148-1197 of SEQ ID NO: 28, at least 80%
amino acid identity with amino acids 1049-1096, amino acids
1110-1138, or amino acids 1148-1197 of SEQ ID NO: 28, at least 85%
amino acid identity with amino acids 1049-1096, amino acids
1110-1138, or amino acids 1148-1197 of SEQ ID NO: 28, at least 90%
amino acid identity with amino acids 1049-1096, amino acids
1110-1138, or amino acids 1148-1197 of SEQ ID NO: 28 or at least
95% amino acid identity with amino acids 1049-1096, amino acids
1110-1138, or amino acids 1148-1197 of SEQ ID NO: 28. In yet other
aspects of this embodiment, a Clostridial botulinum serotype D NTNH
comprising a .beta.-trefoil domain with enhanced binding activity
comprises a polypeptide having, e.g., at most 70% amino acid
identity with amino acids 1049-1096, amino acids 1110-1138, or
amino acids 1148-1197 of SEQ ID NO: 28, at most 75% amino acid
identity with amino acids 1049-1096, amino acids 1110-1138, or
amino acids 1148-1197 of SEQ ID NO: 28, at most 80% amino acid
identity with amino acids 1049-1096, amino acids 1110-1138, or
amino acids 1148-1197 of SEQ ID NO: 28, at most 85% amino acid
identity with amino acids 1049-1096, amino acids 1110-1138, or
amino acids 1148-1197 of SEQ ID NO: 28, at most 90% amino acid
identity with amino acids 1049-1096, amino acids 1110-1138, or
amino acids 1148-1197 of SEQ ID NO: 28 or at most 95% amino acid
identity with amino acids 1049-1096, amino acids 1110-1138, or
amino acids 1148-1197 of SEQ ID NO: 28.
[0550] In other aspects of this embodiment, a Clostridial botulinum
serotype D NTNH comprising a 11-trefoil domain with enhanced
binding activity comprises a polypeptide having, e.g., at most one,
two, three, four, five, six, seven, eight, nine, 10 or 20
non-contiguous amino acid substitutions relative to amino acids
1049-1096, amino acids 1110-1138, or amino acids 1148-1197 of SEQ
ID NO: 28. In other aspects of this embodiment, a Clostridial
botulinum serotype D NTNH comprising a .beta.-trefoil domain with
enhanced binding activity comprises a polypeptide having, e.g., at
least one, two, three, four, five, six, seven, eight, nine, 10 or
20 non-contiguous amino acid substitutions relative to amino acids
1049-1096, amino acids 1110-1138, or amino acids 1148-1197 of SEQ
ID NO: 28. In yet other aspects of this embodiment, a Clostridial
botulinum serotype D NTNH comprising a .beta.-trefoil domain with
enhanced binding activity comprises a polypeptide having, e.g., at
most one, two, three, four, five, six, seven, eight, nine, 10 or 20
non-contiguous amino acid deletions relative to amino acids
1049-1096, amino acids 1110-1138, or amino acids 1148-1197 of SEQ
ID NO: 28. In other aspects of this embodiment, a Clostridial
botulinum serotype D NTNH comprising a .beta.-trefoil domain with
enhanced binding activity comprises a polypeptide having, e.g., at
least one, two, three, four, five, six, seven, eight, nine, 10 or
20 non-contiguous amino acid deletions relative to amino acids
1049-1096, amino acids 1110-1138, or amino acids 1148-1197 of SEQ
ID NO: 28. In still other aspects of this embodiment, a Clostridial
botulinum serotype D NTNH comprising a .beta.-trefoil domain with
enhanced binding activity comprises a polypeptide having, e.g., at
most one, two, three, four, five, six, seven, eight, nine, 10 or 20
non-contiguous amino acid additions relative to amino acids
1049-1096, amino acids 1110-1138, or amino acids 1148-1197 of SEQ
ID NO: 28. In other aspects of this embodiment, a Clostridial
botulinum serotype D NTNH comprising a .beta.-trefoil domain with
enhanced binding activity comprises a polypeptide having, e.g., at
least one, two, three, four, five, six, seven, eight, nine, 10 or
20 non-contiguous amino acid additions relative to amino acids
1049-1096, amino acids 1110-1138, or amino acids 1148-1197 of SEQ
ID NO: 28.
[0551] In other aspects of this embodiment, a Clostridial botulinum
serotype D NTNH comprising a .beta.-trefoil domain with enhanced
binding activity comprises a polypeptide having, e.g., at most one,
two, three, four, five, six, seven, eight, nine, 10 or 20
contiguous amino acid substitutions relative to amino acids
1049-1096, amino acids 1110-1138, or amino acids 1148-1197 of SEQ
ID NO: 28. In other aspects of this embodiment, a Clostridial
botulinum serotype D NTNH comprising a .beta.-trefoil domain with
enhanced binding activity comprises a polypeptide having, e.g., at
least one, two, three, four, five, six, seven, eight, nine, 10 or
20 contiguous amino acid substitutions relative to amino acids
1049-1096, amino acids 1110-1138, or amino acids 1148-1197 of SEQ
ID NO: 28. In yet other aspects of this embodiment, a Clostridial
botulinum serotype D NTNH comprising a .beta.-trefoil domain with
enhanced binding activity comprises a polypeptide having, e.g., at
most one, two, three, four, five, six, seven, eight, nine, 10 or 20
contiguous amino acid deletions relative to amino acids 1049-1096,
amino acids 1110-1138, or amino acids 1148-1197 of SEQ ID NO: 28.
In other aspects of this embodiment, a Clostridial botulinum
serotype D NTNH comprising a .beta.-trefoil domain with enhanced
binding activity comprises a polypeptide having, e.g., at least
one, two, three, four, five, six, seven, eight, nine, 10 or 20
contiguous amino acid deletions relative to amino acids 1049-1096,
amino acids 1110-1138, or amino acids 1148-1197 of SEQ ID NO: 28.
In still other aspects of this embodiment, a Clostridial botulinum
serotype D NTNH comprising a .beta.-trefoil domain with enhanced
binding activity comprises a polypeptide having, e.g., at most one,
two, three, four, five, six, seven, eight, nine, 10 or 20
contiguous amino acid additions relative to amino acids 1049-1096,
amino acids 1110-1138, or amino acids 1148-1197 of SEQ ID NO: 28.
In other aspects of this embodiment, a Clostridial botulinum
serotype D NTNH comprising a .beta.-trefoil domain with enhanced
binding activity comprises a polypeptide having, e.g., at least
one, two, three, four, five, six, seven, eight, nine, 10 or 20
contiguous amino acid additions relative to amino acids 1049-1096,
amino acids 1110-1138, or amino acids 1148-1197 of SEQ ID NO:
28.
[0552] In another embodiment, a .beta.-trefoil domain derived from
a Clostridial botulinum serotype D NTNH comprises a .beta.4/.beta.5
hairpin turn of a .beta.-trefoil domain of a Clostridial botulinum
serotype D NTNH or a .beta.8/.beta.9 hairpin turn of a
.beta.-trefoil domain of a Clostridial botulinum serotype D NTNH of
SEQ ID NO: 28. In another aspect of this embodiment, a
.beta.-trefoil domain derived from a Clostridial botulinum serotype
D NTNH comprises amino acids 1097-1109 or amino acids 1139-1147 of
SEQ ID NO: 28.
[0553] In another embodiment, a .beta.-trefoil domain with enhanced
binding activity derived from a Clostridial botulinum serotype D
NTNH comprises a modified .beta.4/.beta.5 hairpin turn of a
.beta.-trefoil domain of a Clostridial botulinum serotype D NTNH or
a modified .beta.8/.beta.9 hairpin turn of a .beta.-trefoil domain
of a Clostridial botulinum serotype D NTNH of SEQ ID NO: 28. In
another aspect of this embodiment, a .beta.-trefoil domain with
enhanced binding activity derived from a Clostridial botulinum
serotype D NTNH comprises a modification of amino acids 1097-1109
or amino acids 1139-1147 of SEQ ID NO: 28.
[0554] In other aspects of this embodiment, a .beta.-trefoil domain
with enhanced binding activity derived from a Clostridial botulinum
serotype D NTNH comprises a polypeptide having, e.g., at least 70%
amino acid identity with amino acids 1097-1109 or amino acids
1139-1147 of SEQ ID NO: 28, at least 75% amino acid identity with
amino acids 1097-1109 or amino acids 1139-1147 of SEQ ID NO: 28, at
least 80% amino acid identity with amino acids 1097-1109 or amino
acids 1139-1147 of SEQ ID NO: 28, at least 85% amino acid identity
with amino acids 1097-1109 or amino acids 1139-1147 of SEQ ID NO:
28, at least 90% amino acid identity with amino acids 1097-1109 or
amino acids 1139-1147 of SEQ ID NO: 28 or at least 95% amino acid
identity with amino acids 1097-1109 or amino acids 1139-1147 of SEQ
ID NO: 28. In yet other aspects of this embodiment, a
.beta.-trefoil domain with enhanced binding activity derived from a
Clostridial botulinum serotype D NTNH comprises a polypeptide
having, e.g., at most 70% amino acid identity with amino acids
1097-1109 or amino acids 1139-1147 of SEQ ID NO: 28, at most 75%
amino acid identity with amino acids 1097-1109 or amino acids
1139-1147 of SEQ ID NO: 28, at most 80% amino acid identity with
amino acids 1097-1109 or amino acids 1139-1147 of SEQ ID NO: 28, at
most 85% amino acid identity with amino acids 1097-1109 or amino
acids 1139-1147 of SEQ ID NO: 28, at most 90% amino acid identity
with amino acids 1097-1109 or amino acids 1139-1147 of SEQ ID NO:
28 or at most 95% amino acid identity with amino acids 1097-1109 or
amino acids 1139-1147 of SEQ ID NO: 28.
[0555] In other aspects of this embodiment, a .beta.-trefoil domain
with enhanced binding activity derived from a Clostridial botulinum
serotype D NTNH comprises a polypeptide having, e.g., at most one,
two, three or four non-contiguous amino acid substitutions relative
to amino acids 1097-1109 or amino acids 1139-1147 of SEQ ID NO: 28.
In other aspects of this embodiment, a .beta.-trefoil domain with
enhanced binding activity derived from a Clostridial botulinum
serotype D NTNH comprises a polypeptide having, e.g., at least one,
two, three or four non-contiguous amino acid substitutions relative
to amino acids 1097-1109 or amino acids 1139-1147 of SEQ ID NO: 28.
In other aspects of this embodiment, a non-contiguous amino acid
substitution of any amino acid from amino acids 1097-1109 or amino
acids 1139-1147 of SEQ ID NO: 28 can be replaced with glycine. In
other aspects of this embodiment, a non-contiguous amino acid
substitution of any hydrophobic amino acid from amino acids
1097-1109 or amino acids 1139-1147 of SEQ ID NO: 28 can be replaced
with phenylalanine. In yet other aspects of this embodiment, a
.beta.-trefoil domain with enhanced binding activity derived from a
Clostridial botulinum serotype D NTNH comprises a polypeptide
having, e.g., at most one, two, three or four non-contiguous amino
acid deletions relative to amino acids 1097-1109 or amino acids
1139-1147 of SEQ ID NO: 28. In other aspects of this embodiment, a
.beta.-trefoil domain with enhanced binding activity derived from a
Clostridial botulinum serotype D NTNH comprises a polypeptide
having, e.g., at least one, two, three or four non-contiguous amino
acid deletions relative to amino acids 1097-1109 or amino acids
1139-1147 of SEQ ID NO: 28. In still other aspects of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a Clostridial botulinum serotype D NTNH comprises a
polypeptide having, e.g., at most one, two, three or four
non-contiguous amino acid additions relative to amino acids
1097-1109 or amino acids 1139-1147 of SEQ ID NO: 28. In other
aspects of this embodiment, a .beta.-trefoil domain with enhanced
binding activity derived from a Clostridial botulinum serotype D
NTNH comprises a polypeptide having, e.g., at least one, two, three
or four non-contiguous amino acid additions relative to amino acids
1097-1109 or amino acids 1139-1147 of SEQ ID NO: 28.
[0556] In other aspects of this embodiment, a .beta.-trefoil domain
with enhanced binding activity derived from a Clostridial botulinum
serotype D NTNH comprises a polypeptide having, e.g., at most one,
two, three or four contiguous amino acid substitutions relative to
amino acids 1097-1109 or amino acids 1139-1147 of SEQ ID NO: 28. In
other aspects of this embodiment, a .beta.-trefoil domain with
enhanced binding activity derived from a Clostridial botulinum
serotype D NTNH comprises a polypeptide having, e.g., at least one,
two, three or four contiguous amino acid substitutions relative to
amino acids 1097-1109 or amino acids 1139-1147 of SEQ ID NO: 28. In
other aspects of this embodiment, contiguous amino acid
substitutions of amino acids from amino acids 1097-1109 or amino
acids 1139-1147 of SEQ ID NO: 28 can be replaced with glycine. In
other aspects of this embodiment, contiguous amino acid
substitutions of hydrophobic amino acids from amino acids 1097-1109
or amino acids 1139-1147 of SEQ ID NO: 28 can be replaced with
phenylalanine. In yet other aspects of this embodiment, a
.beta.-trefoil domain with enhanced binding activity derived from a
Clostridial botulinum serotype D NTNH comprises a polypeptide
having, e.g., at most one, two, three or four contiguous amino acid
deletions relative to amino acids 1097-1109 or amino acids
1139-1147 of SEQ ID NO: 28. In other aspects of this embodiment, a
.beta.-trefoil domain with enhanced binding activity derived from a
Clostridial botulinum serotype D NTNH comprises a polypeptide
having, e.g., at least one, two, three or four contiguous amino
acid deletions relative to amino acids 1097-1109 or amino acids
1139-1147 of SEQ ID NO: 28. In still other aspects of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a Clostridial botulinum serotype D NTNH comprises a
polypeptide having, e.g., at most one, two, three or four
contiguous amino acid additions relative to amino acids 1097-1109
or amino acids 1139-1147 of SEQ ID NO: 28. In other aspects of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a Clostridial botulinum serotype D NTNH comprises a
polypeptide having, e.g., at least one, two, three or four
contiguous amino acid additions relative to amino acids 1097-1109
or amino acids 1139-1147 of SEQ ID NO: 28.
[0557] In another embodiment, a .beta.-trefoil domain derived from
a Clostridial botulinum serotype E NTNH comprises a .beta.-trefoil
domain derived from a Clostridial botulinum serotype E NTNH of SEQ
ID NO: 29. In another embodiment, a .beta.-trefoil domain derived
from a Clostridial botulinum serotype E NTNH comprises amino acids
1014-1163 of SEQ ID NO: 29. In another aspect of this embodiment, a
.beta.-trefoil domain derived from a Clostridial botulinum serotype
E NTNH comprises a .alpha.-fold motif of a .beta.-trefoil domain of
a Clostridial botulinum serotype E NTNH, a .beta.-fold motif of a
.beta.-trefoil domain of a Clostridial botulinum serotype E NTNH or
a .gamma.-fold motif of a .beta.-trefoil domain of a Clostridial
botulinum serotype E NTNH of SEQ ID NO: 29. In another aspect of
this embodiment, a .beta.-trefoil domain derived from a Clostridial
botulinum serotype E NTNH comprises amino acids 1014-1061, amino
acids 1075-1103, or amino acids 1114-1163 of SEQ ID NO: 29.
[0558] In another embodiment, a .beta.-trefoil domain with enhanced
binding activity derived from a Clostridial botulinum serotype E
NTNH comprises a .beta.-trefoil domain with enhanced binding
activity derived from a Clostridial botulinum serotype E NTNH of
SEQ ID NO: 29. In another embodiment, a .beta.-trefoil domain with
enhanced binding activity derived from a Clostridial botulinum
serotype E NTNH comprises amino acids 1014-1163 of SEQ ID NO: 29.
In another aspect of this embodiment, a .beta.-trefoil domain with
enhanced binding activity derived from a Clostridial botulinum
serotype E NTNH comprises a modified .alpha.-fold motif of a
.beta.-trefoil domain of a Clostridial botulinum serotype E NTNH, a
modified .beta.-fold motif of a .beta.-trefoil domain of a
Clostridial botulinum serotype E NTNH or a modified .gamma.-fold
motif of a 13-trefoil domain of a Clostridial botulinum serotype E
NTNH of SEQ ID NO: 29. In another aspect of this embodiment, a
.beta.-trefoil domain with enhanced binding activity derived from a
Clostridial botulinum serotype E NTNH comprises amino acids
1014-1061, amino acids 1075-1103, or amino acids 1114-1163 of SEQ
ID NO: 29.
[0559] In other aspects of this embodiment, a Clostridial botulinum
serotype E NTNH comprising a 11-trefoil domain with enhanced
binding activity comprises a polypeptide having, e.g., at least 70%
amino acid identity with amino acids 1014-1061, amino acids
1075-1103, or amino acids 1114-1163 of SEQ ID NO: 29, at least 75%
amino acid identity with amino acids 1014-1061, amino acids
1075-1103, or amino acids 1114-1163 of SEQ ID NO: 29, at least 80%
amino acid identity with amino acids 1014-1061, amino acids
1075-1103, or amino acids 1114-1163 of SEQ ID NO: 29, at least 85%
amino acid identity with amino acids 1014-1061, amino acids
1075-1103, or amino acids 1114-1163 of SEQ ID NO: 29, at least 90%
amino acid identity with amino acids 1014-1061, amino acids
1075-1103, or amino acids 1114-1163 of SEQ ID NO: 29 or at least
95% amino acid identity with amino acids 1014-1061, amino acids
1075-1103, or amino acids 1114-1163 of SEQ ID NO: 29. In yet other
aspects of this embodiment, a Clostridial botulinum serotype E NTNH
comprising a .beta.-trefoil domain with enhanced binding activity
comprises a polypeptide having, e.g., at most 70% amino acid
identity with amino acids 1014-1061, amino acids 1075-1103, or
amino acids 1114-1163 of SEQ ID NO: 29, at most 75% amino acid
identity with amino acids 1014-1061, amino acids 1075-1103, or
amino acids 1114-1163 of SEQ ID NO: 29, at most 80% amino acid
identity with amino acids 1014-1061, amino acids 1075-1103, or
amino acids 1114-1163 of SEQ ID NO: 29, at most 85% amino acid
identity with amino acids 1014-1061, amino acids 1075-1103, or
amino acids 1114-1163 of SEQ ID NO: 29, at most 90% amino acid
identity with amino acids 1014-1061, amino acids 1075-1103, or
amino acids 1114-1163 of SEQ ID NO: 29 or at most 95% amino acid
identity with amino acids 1014-1061, amino acids 1075-1103, or
amino acids 1114-1163 of SEQ ID NO: 29.
[0560] In other aspects of this embodiment, a Clostridial botulinum
serotype E NTNH comprising a .beta.-trefoil domain with enhanced
binding activity comprises a polypeptide having, e.g., at most one,
two, three, four, five, six, seven, eight, nine, 10 or 20
non-contiguous amino acid substitutions relative to amino acids
1014-1061, amino acids 1075-1103, or amino acids 1114-1163 of SEQ
ID NO: 29. In other aspects of this embodiment, a Clostridial
botulinum serotype E NTNH comprising a .beta.-trefoil domain with
enhanced binding activity comprises a polypeptide having, e.g., at
least one, two, three, four, five, six, seven, eight, nine, 10 or
20 non-contiguous amino acid substitutions relative to amino acids
1014-1061, amino acids 1075-1103, or amino acids 1114-1163 of SEQ
ID NO: 29. In yet other aspects of this embodiment, a Clostridial
botulinum serotype E NTNH comprising a .beta.-trefoil domain with
enhanced binding activity comprises a polypeptide having, e.g., at
most one, two, three, four, five, six, seven, eight, nine, 10 or 20
non-contiguous amino acid deletions relative to amino acids
1014-1061, amino acids 1075-1103, or amino acids 1114-1163 of SEQ
ID NO: 29. In other aspects of this embodiment, a Clostridial
botulinum serotype E NTNH comprising a .beta.-trefoil domain with
enhanced binding activity comprises a polypeptide having, e.g., at
least one, two, three, four, five, six, seven, eight, nine, 10 or
20 non-contiguous amino acid deletions relative to amino acids
1014-1061, amino acids 1075-1103, or amino acids 1114-1163 of SEQ
ID NO: 29. In still other aspects of this embodiment, a Clostridial
botulinum serotype E NTNH comprising a .beta.-trefoil domain with
enhanced binding activity comprises a polypeptide having, e.g., at
most one, two, three, four, five, six, seven, eight, nine, 10 or 20
non-contiguous amino acid additions relative to amino acids
1014-1061, amino acids 1075-1103, or amino acids 1114-1163 of SEQ
ID NO: 29. In other aspects of this embodiment, a Clostridial
botulinum serotype E NTNH comprising a .beta.-trefoil domain with
enhanced binding activity comprises a polypeptide having, e.g., at
least one, two, three, four, five, six, seven, eight, nine, 10 or
20 non-contiguous amino acid additions relative to amino acids
1014-1061, amino acids 1075-1103, or amino acids 1114-1163 of SEQ
ID NO: 29.
[0561] In other aspects of this embodiment, a Clostridial botulinum
serotype E NTNH comprising a Ii-trefoil domain with enhanced
binding activity comprises a polypeptide having, e.g., at most one,
two, three, four, five, six, seven, eight, nine, 10 or 20
contiguous amino acid substitutions relative to amino acids
1014-1061, amino acids 1075-1103, or amino acids 1114-1163 of SEQ
ID NO: 29. In other aspects of this embodiment, a Clostridial
botulinum serotype E NTNH comprising a .beta.-trefoil domain with
enhanced binding activity comprises a polypeptide having, e.g., at
least one, two, three, four, five, six, seven, eight, nine, 10 or
20 contiguous amino acid substitutions relative to amino acids
1014-1061, amino acids 1075-1103, or amino acids 1114-1163 of SEQ
ID NO: 29. In yet other aspects of this embodiment, a Clostridial
botulinum serotype E NTNH comprising a .beta.-trefoil domain with
enhanced binding activity comprises a polypeptide having, e.g., at
most one, two, three, four, five, six, seven, eight, nine, 10 or 20
contiguous amino acid deletions relative to amino acids 1014-1061,
amino acids 1075-1103, or amino acids 1114-1163 of SEQ ID NO: 29.
In other aspects of this embodiment, a Clostridial botulinum
serotype E NTNH comprising a .beta.-trefoil domain with enhanced
binding activity comprises a polypeptide having, e.g., at least
one, two, three, four, five, six, seven, eight, nine, 10 or 20
contiguous amino acid deletions relative to amino acids 1014-1061,
amino acids 1075-1103, or amino acids 1114-1163 of SEQ ID NO: 29.
In still other aspects of this embodiment, a Clostridial botulinum
serotype E NTNH comprising a .beta.-trefoil domain with enhanced
binding activity comprises a polypeptide having, e.g., at most one,
two, three, four, five, six, seven, eight, nine, 10 or 20
contiguous amino acid additions relative to amino acids 1014-1061,
amino acids 1075-1103, or amino acids 1114-1163 of SEQ ID NO: 29.
In other aspects of this embodiment, a Clostridial botulinum
serotype E NTNH comprising a .beta.-trefoil domain with enhanced
binding activity comprises a polypeptide having, e.g., at least
one, two, three, four, five, six, seven, eight, nine, 10 or 20
contiguous amino acid additions relative to amino acids 1014-1061,
amino acids 1075-1103, or amino acids 1114-1163 of SEQ ID NO:
29.
[0562] In another embodiment, a .beta.-trefoil domain derived from
a Clostridial botulinum serotype E NTNH comprises a
8.beta.4/.beta.5 hairpin turn of a .beta.-trefoil domain of a
Clostridial botulinum serotype E NTNH or a .beta.8/.beta.9 hairpin
turn of a .beta.-trefoil domain of a Clostridial botulinum serotype
E NTNH of SEQ ID NO: 29. In another aspect of this embodiment, a
.beta.-trefoil domain derived from a Clostridial botulinum serotype
E NTNH comprises amino acids 1062-1074 or amino acids 1104-1113 of
SEQ ID NO: 29.
[0563] In another embodiment, a .beta.-trefoil domain with enhanced
binding activity derived from a Clostridial botulinum serotype E
NTNH comprises a modified 8.beta.4/.beta.5 hairpin turn of a
.beta.-trefoil domain of a Clostridial botulinum serotype E NTNH or
a modified 8.beta.8/.beta.9 hairpin turn of a .beta.-trefoil domain
of a Clostridial botulinum serotype E NTNH of SEQ ID NO: 29. In
another aspect of this embodiment, a R-trefoil domain with enhanced
binding activity derived from a Clostridial botulinum serotype E
NTNH comprises a modification of amino acids 1062-1074 or amino
acids 1104-1113 of SEQ ID NO: 29.
[0564] In other aspects of this embodiment, a .beta.-trefoil domain
with enhanced binding activity derived from a Clostridial botulinum
serotype E NTNH comprises a polypeptide having, e.g., at least 70%
amino acid identity with amino acids 1062-1074 or amino acids
1104-1113 of SEQ ID NO: 29, at least 75% amino acid identity with
amino acids 1062-1074 or amino acids 1104-1113 of SEQ ID NO: 29, at
least 80% amino acid identity with amino acids 1062-1074 or amino
acids 1104-1113 of SEQ ID NO: 29, at least 85% amino acid identity
with amino acids 1062-1074 or amino acids 1104-1113 of SEQ ID NO:
29, at least 90% amino acid identity with amino acids 1062-1074 or
amino acids 1104-1113 of SEQ ID NO: 29 or at least 95% amino acid
identity with amino acids 1062-1074 or amino acids 1104-1113 of SEQ
ID NO: 29. In yet other aspects of this embodiment, a
.beta.-trefoil domain with enhanced binding activity derived from a
Clostridial botulinum serotype E NTNH comprises a polypeptide
having, e.g., at most 70% amino acid identity with amino acids
1062-1074 or amino acids 1104-1113 of SEQ ID NO: 29, at most 75%
amino acid identity with amino acids 1062-1074 or amino acids
1104-1113 of SEQ ID NO: 29, at most 80% amino acid identity with
amino acids 1062-1074 or amino acids 1104-1113 of SEQ ID NO: 29, at
most 85% amino acid identity with amino acids 1062-1074 or amino
acids 1104-1113 of SEQ ID NO: 29, at most 90% amino acid identity
with amino acids 1062-1074 or amino acids 1104-1113 of SEQ ID NO:
29 or at most 95% amino acid identity with amino acids 1062-1074 or
amino acids 1104-1113 of SEQ ID NO: 29.
[0565] In other aspects of this embodiment, a .beta.-trefoil domain
with enhanced binding activity derived from a Clostridial botulinum
serotype E NTNH comprises a polypeptide having, e.g., at most one,
two, three or four non-contiguous amino acid substitutions relative
to amino acids 1062-1074 or amino acids 1104-1113 of SEQ ID NO: 29.
In other aspects of this embodiment, a .beta.-trefoil domain with
enhanced binding activity derived from a Clostridial botulinum
serotype E NTNH comprises a polypeptide having, e.g., at least one,
two, three or four non-contiguous amino acid substitutions relative
to amino acids 1062-1074 or amino acids 1104-1113 of SEQ ID NO: 29.
In other aspects of this embodiment, a non-contiguous amino acid
substitution of any amino acid from amino acids 1062-1074 or amino
acids 1104-1113 of SEQ ID NO: 29 can be replaced with glycine. In
other aspects of this embodiment, a non-contiguous amino acid
substitution of any hydrophobic amino acid from amino acids
1062-1074 or amino acids 1104-1113 of SEQ ID NO: 29 can be replaced
with phenylalanine. In yet other aspects of this embodiment, a
.beta.-trefoil domain with enhanced binding activity derived from a
Clostridial botulinum serotype E NTNH comprises a polypeptide
having, e.g., at most one, two, three or four non-contiguous amino
acid deletions relative to amino acids 1062-1074 or amino acids
1104-1113 of SEQ ID NO: 29. In other aspects of this embodiment, a
.beta.-trefoil domain with enhanced binding activity derived from a
Clostridial botulinum serotype E NTNH comprises a polypeptide
having, e.g., at least one, two, three or four non-contiguous amino
acid deletions relative to amino acids 1062-1074 or amino acids
1104-1113 of SEQ ID NO: 29. In still other aspects of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a Clostridial botulinum serotype E NTNH comprises a
polypeptide having, e.g., at most one, two, three or four
non-contiguous amino acid additions relative to amino acids
1062-1074 or amino acids 1104-1113 of SEQ ID NO: 29. In other
aspects of this embodiment, a .beta.-trefoil domain with enhanced
binding activity derived from a Clostridial botulinum serotype E
NTNH comprises a polypeptide having, e.g., at least one, two, three
or four non-contiguous amino acid additions relative to amino acids
1062-1074 or amino acids 1104-1113 of SEQ ID NO: 29.
[0566] In other aspects of this embodiment, a .beta.-trefoil domain
with enhanced binding activity derived from a Clostridial botulinum
serotype E NTNH comprises a polypeptide having, e.g., at most one,
two, three or four contiguous amino acid substitutions relative to
amino acids 1062-1074 or amino acids 1104-1113 of SEQ ID NO: 29. In
other aspects of this embodiment, a .beta.-trefoil domain with
enhanced binding activity derived from a Clostridial botulinum
serotype E NTNH comprises a polypeptide having, e.g., at least one,
two, three or four contiguous amino acid substitutions relative to
amino acids 1062-1074 or amino acids 1104-1113 of SEQ ID NO: 29. In
other aspects of this embodiment, contiguous amino acid
substitutions of amino acids from amino acids 1062-1074 or amino
acids 1104-1113 of SEQ ID NO: 29 can be replaced with glycine. In
other aspects of this embodiment, contiguous amino acid
substitutions of hydrophobic amino acids from amino acids 1062-1074
or amino acids 1104-1113 of SEQ ID NO: 29 can be replaced with
phenylalanine. In yet other aspects of this embodiment, a
.beta.-trefoil domain with enhanced binding activity derived from a
Clostridial botulinum serotype E NTNH comprises a polypeptide
having, e.g., at most one, two, three or four contiguous amino acid
deletions relative to amino acids 1062-1074 or amino acids
1104-1113 of SEQ ID NO: 29. In other aspects of this embodiment, a
.beta.-trefoil domain with enhanced binding activity derived from a
Clostridial botulinum serotype E NTNH comprises a polypeptide
having, e.g., at least one, two, three or four contiguous amino
acid deletions relative to amino acids 1062-1074 or amino acids
1104-1113 of SEQ ID NO: 29. In still other aspects of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a Clostridial botulinum serotype E NTNH comprises a
polypeptide having, e.g., at most one, two, three or four
contiguous amino acid additions relative to amino acids 1062-1074
or amino acids 1104-1113 of SEQ ID NO: 29. In other aspects of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a Clostridial botulinum serotype E NTNH comprises a
polypeptide having, e.g., at least one, two, three or four
contiguous amino acid additions relative to amino acids 1062-1074
or amino acids 1104-1113 of SEQ ID NO: 29.
[0567] In another embodiment, a .beta.-trefoil domain derived from
a Clostridial botulinum serotype F NTNH comprises a .beta.-trefoil
domain derived from a Clostridial botulinum serotype F NTNH of SEQ
ID NO: 30. In another embodiment, a .beta.-trefoil domain derived
from a Clostridial botulinum serotype F NTNH comprises amino acids
1016-1160 of SEQ ID NO: 30. In another aspect of this embodiment, a
.beta.-trefoil domain derived from a Clostridial botulinum serotype
F NTNH comprises a .alpha.-fold motif of a .beta.-trefoil domain of
a Clostridial botulinum serotype F NTNH, a .beta.-fold motif of a
.beta.-trefoil domain of a Clostridial botulinum serotype F NTNH or
a .gamma.-fold motif of a .beta.-trefoil domain of a Clostridial
botulinum serotype F NTNH of SEQ ID NO: 30. In another aspect of
this embodiment, a .beta.-trefoil domain derived from a Clostridial
botulinum serotype F NTNH comprises amino acids 1016-1063, amino
acids 1077-1104, or amino acids 1115-1160 of SEQ ID NO: 30.
[0568] In another embodiment, a .beta.-trefoil domain with enhanced
binding activity derived from a Clostridial botulinum serotype F
NTNH comprises a .beta.-trefoil domain with enhanced binding
activity derived from a Clostridial botulinum serotype F NTNH of
SEQ ID NO: 30. In another embodiment, a .beta.-trefoil domain with
enhanced binding activity derived from a Clostridial botulinum
serotype F NTNH comprises amino acids 1016-1160 of SEQ ID NO: 30.
In another aspect of this embodiment, a .beta.-trefoil domain with
enhanced binding activity derived from a Clostridial botulinum
serotype F NTNH comprises a modified .alpha.-fold motif of a
.beta.-trefoil domain of a Clostridial botulinum serotype F NTNH, a
modified .beta.-fold motif of a .beta.-trefoil domain of a
Clostridial botulinum serotype F NTNH or a modified .gamma.-fold
motif of a .beta.-trefoil domain of a Clostridial botulinum
serotype F NTNH of SEQ ID NO: 30. In another aspect of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a Clostridial botulinum serotype F NTNH comprises
amino acids 1016-1063, amino acids 1077-1104, or amino acids
1115-1160 of SEQ ID NO: 30.
[0569] In other aspects of this embodiment, a Clostridial botulinum
serotype F NTNH comprising a .beta.-trefoil domain with enhanced
binding activity comprises a polypeptide having, e.g., at least 70%
amino acid identity with amino acids 1016-1063, amino acids
1077-1104, or amino acids 1115-1160 of SEQ ID NO: 30, at least 75%
amino acid identity with amino acids 1016-1063, amino acids
1077-1104, or amino acids 1115-1160 of SEQ ID NO: 30, at least 80%
amino acid identity with amino acids 1016-1063, amino acids
1077-1104, or amino acids 1115-1160 of SEQ ID NO: 30, at least 85%
amino acid identity with amino acids 1016-1063, amino acids
1077-1104, or amino acids 1115-1160 of SEQ ID NO: 30, at least 90%
amino acid identity with amino acids 1016-1063, amino acids
1077-1104, or amino acids 1115-1160 of SEQ ID NO: 30 or at least
95% amino acid identity with amino acids 1016-1063, amino acids
1077-1104, or amino acids 1115-1160 of SEQ ID NO: 30. In yet other
aspects of this embodiment, a Clostridial botulinum serotype F NTNH
comprising a .beta.-trefoil domain with enhanced binding activity
comprises a polypeptide having, e.g., at most 70% amino acid
identity with amino acids 1016-1063, amino acids 1077-1104, or
amino acids 1115-1160 of SEQ ID NO: 30, at most 75% amino acid
identity with amino acids 1016-1063, amino acids 1077-1104, or
amino acids 1115-1160 of SEQ ID NO: 30, at most 80% amino acid
identity with amino acids 1016-1063, amino acids 1077-1104, or
amino acids 1115-1160 of SEQ ID NO: 30, at most 85% amino acid
identity with amino acids 1016-1063, amino acids 1077-1104, or
amino acids 1115-1160 of SEQ ID NO: 30, at most 90% amino acid
identity with amino acids 1016-1063, amino acids 1077-1104, or
amino acids 1115-1160 of SEQ ID NO: 30 or at most 95% amino acid
identity with amino acids 1016-1063, amino acids 1077-1104, or
amino acids 1115-1160 of SEQ ID NO: 30.
[0570] In other aspects of this embodiment, a Clostridial botulinum
serotype F NTNH comprising a .beta.-trefoil domain with enhanced
binding activity comprises a polypeptide having, e.g., at most one,
two, three, four, five, six, seven, eight, nine, 10 or 20
non-contiguous amino acid substitutions relative to amino acids
1016-1063, amino acids 1077-1104, or amino acids 1115-1160 of SEQ
ID NO: 30. In other aspects of this embodiment, a Clostridial
botulinum serotype F NTNH comprising a .beta.-trefoil domain with
enhanced binding activity comprises a polypeptide having, e.g., at
least one, two, three, four, five, six, seven, eight, nine, 10 or
20 non-contiguous amino acid substitutions relative to amino acids
1016-1063, amino acids 1077-1104, or amino acids 1115-1160 of SEQ
ID NO: 30. In yet other aspects of this embodiment, a Clostridial
botulinum serotype F NTNH comprising a .beta.-trefoil domain with
enhanced binding activity comprises a polypeptide having, e.g., at
most one, two, three, four, five, six, seven, eight, nine, 10 or 20
non-contiguous amino acid deletions relative to amino acids
1016-1063, amino acids 1077-1104, or amino acids 1115-1160 of SEQ
ID NO: 30. In other aspects of this embodiment, a Clostridial
botulinum serotype F NTNH comprising a .beta.-trefoil domain with
enhanced binding activity comprises a polypeptide having, e.g., at
least one, two, three, four, five, six, seven, eight, nine, 10 or
20 non-contiguous amino acid deletions relative to amino acids
1016-1063, amino acids 1077-1104, or amino acids 1115-1160 of SEQ
ID NO: 30. In still other aspects of this embodiment, a Clostridial
botulinum serotype F NTNH comprising a .beta.-trefoil domain with
enhanced binding activity comprises a polypeptide having, e.g., at
most one, two, three, four, five, six, seven, eight, nine, 10 or 20
non-contiguous amino acid additions relative to amino acids
1016-1063, amino acids 1077-1104, or amino acids 1115-1160 of SEQ
ID NO: 30. In other aspects of this embodiment, a Clostridial
botulinum serotype F NTNH comprising a .beta.-trefoil domain with
enhanced binding activity comprises a polypeptide having, e.g., at
least one, two, three, four, five, six, seven, eight, nine, 10 or
20 non-contiguous amino acid additions relative to amino acids
1016-1063, amino acids 1077-1104, or amino acids 1115-1160 of SEQ
ID NO: 30.
[0571] In other aspects of this embodiment, a Clostridial botulinum
serotype F NTNH comprising a .beta.-trefoil domain with enhanced
binding activity comprises a polypeptide having, e.g., at most one,
two, three, four, five, six, seven, eight, nine, 10 or 20
contiguous amino acid substitutions relative to amino acids
1016-1063, amino acids 1077-1104, or amino acids 1115-1160 of SEQ
ID NO: 30. In other aspects of this embodiment, a Clostridial
botulinum serotype F NTNH comprising a .beta.-trefoil domain with
enhanced binding activity comprises a polypeptide having, e.g., at
least one, two, three, four, five, six, seven, eight, nine, 10 or
20 contiguous amino acid substitutions relative to amino acids
1016-1063, amino acids 1077-1104, or amino acids 1115-1160 of SEQ
ID NO: 30. In yet other aspects of this embodiment, a Clostridial
botulinum serotype F NTNH comprising a .beta.-trefoil domain with
enhanced binding activity comprises a polypeptide having, e.g., at
most one, two, three, four, five, six, seven, eight, nine, 10 or 20
contiguous amino acid deletions relative to amino acids 1016-1063,
amino acids 1077-1104, or amino acids 1115-1160 of SEQ ID NO: 30.
In other aspects of this embodiment, a Clostridial botulinum
serotype F NTNH comprising a .beta.-trefoil domain with enhanced
binding activity comprises a polypeptide having, e.g., at least
one, two, three, four, five, six, seven, eight, nine, 10 or 20
contiguous amino acid deletions relative to amino acids 1016-1063,
amino acids 1077-1104, or amino acids 1115-1160 of SEQ ID NO: 30.
In still other aspects of this embodiment, a Clostridial botulinum
serotype F NTNH comprising a .beta.-trefoil domain with enhanced
binding activity comprises a polypeptide having, e.g., at most one,
two, three, four, five, six, seven, eight, nine, 10 or 20
contiguous amino acid additions relative to amino acids 1016-1063,
amino acids 1077-1104, or amino acids 1115-1160 of SEQ ID NO: 30.
In other aspects of this embodiment, a Clostridial botulinum
serotype F NTNH comprising a .beta.-trefoil domain with enhanced
binding activity comprises a polypeptide having, e.g., at least
one, two, three, four, five, six, seven, eight, nine, 10 or 20
contiguous amino acid additions relative to amino acids 1016-1063,
amino acids 1077-1104, or amino acids 1115-1160 of SEQ ID NO:
30.
[0572] In another embodiment, a .beta.-trefoil domain derived from
a Clostridial botulinum serotype F NTNH comprises a .beta.4/.beta.5
hairpin turn of a .beta.-trefoil domain of a Clostridial botulinum
serotype F NTNH or a 138/139 hairpin turn of a .beta.-trefoil
domain of a Clostridial botulinum serotype F NTNH of SEQ ID NO: 30.
In another aspect of this embodiment, a .beta.-trefoil domain
derived from a Clostridial botulinum serotype F NTNH comprises
amino acids 1064-1076 or amino acids 1105-1114 of SEQ ID NO:
30.
[0573] In another embodiment, a .beta.-trefoil domain with enhanced
binding activity derived from a Clostridial botulinum serotype F
NTNH comprises a modified .beta.4/.beta.5 hairpin turn of a
.beta.-trefoil domain of a Clostridial botulinum serotype F NTNH or
a modified .beta.8/.beta.9 hairpin turn of a .beta.-trefoil domain
of a Clostridial botulinum serotype F NTNH of SEQ ID NO: 30. In
another aspect of this embodiment, a .beta.-trefoil domain with
enhanced binding activity derived from a Clostridial botulinum
serotype F NTNH comprises a modification of amino acids 1064-1076
or amino acids 1105-1114 of SEQ ID NO: 30.
[0574] In other aspects of this embodiment, a .beta.-trefoil domain
with enhanced binding activity derived from a Clostridial botulinum
serotype F NTNH comprises a polypeptide having, e.g., at least 70%
amino acid identity with amino acids 1064-1076 or amino acids
1105-1114 of SEQ ID NO: 30, at least 75% amino acid identity with
amino acids 1064-1076 or amino acids 1105-1114 of SEQ ID NO: 30, at
least 80% amino acid identity with amino acids 1064-1076 or amino
acids 1105-1114 of SEQ ID NO: 30, at least 85% amino acid identity
with amino acids 1064-1076 or amino acids 1105-1114 of SEQ ID NO:
30, at least 90% amino acid identity with amino acids 1064-1076 or
amino acids 1105-1114 of SEQ ID NO: 30 or at least 95% amino acid
identity with amino acids 1064-1076 or amino acids 1105-1114 of SEQ
ID NO: 30. In yet other aspects of this embodiment, a
.beta.-trefoil domain with enhanced binding activity derived from a
Clostridial botulinum serotype F NTNH comprises a polypeptide
having, e.g., at most 70% amino acid identity with amino acids
1064-1076 or amino acids 1105-1114 of SEQ ID NO: 30, at most 75%
amino acid identity with amino acids 1064-1076 or amino acids
1105-1114 of SEQ ID NO: 30, at most 80% amino acid identity with
amino acids 1064-1076 or amino acids 1105-1114 of SEQ ID NO: 30, at
most 85% amino acid identity with amino acids 1064-1076 or amino
acids 1105-1114 of SEQ ID NO: 30, at most 90% amino acid identity
with amino acids 1064-1076 or amino acids 1105-1114 of SEQ ID NO:
30 or at most 95% amino acid identity with amino acids 1064-1076 or
amino acids 1105-1114 of SEQ ID NO: 30.
[0575] In other aspects of this embodiment, a .beta.-trefoil domain
with enhanced binding activity derived from a Clostridial botulinum
serotype F NTNH comprises a polypeptide having, e.g., at most one,
two, three or four non-contiguous amino acid substitutions relative
to amino acids 1064-1076 or amino acids 1105-1114 of SEQ ID NO: 30.
In other aspects of this embodiment, a .beta.-trefoil domain with
enhanced binding activity derived from a Clostridial botulinum
serotype F NTNH comprises a polypeptide having, e.g., at least one,
two, three or four non-contiguous amino acid substitutions relative
to amino acids 1064-1076 or amino acids 1105-1114 of SEQ ID NO: 30.
In other aspects of this embodiment, a non-contiguous amino acid
substitution of any amino acid from amino acids 1064-1076 or amino
acids 1105-1114 of SEQ ID NO: 30 can be replaced with glycine. In
other aspects of this embodiment, a non-contiguous amino acid
substitution of any hydrophobic amino acid from amino acids
1064-1076 or amino acids 1105-1114 of SEQ ID NO: 30 can be replaced
with phenylalanine. In yet other aspects of this embodiment, a
.beta.-trefoil domain with enhanced binding activity derived from a
Clostridial botulinum serotype F NTNH comprises a polypeptide
having, e.g., at most one, two, three or four non-contiguous amino
acid deletions relative to amino acids 1064-1076 or amino acids
1105-1114 of SEQ ID NO: 30. In other aspects of this embodiment, a
.beta.-trefoil domain with enhanced binding activity derived from a
Clostridial botulinum serotype F NTNH comprises a polypeptide
having, e.g., at least one, two, three or four non-contiguous amino
acid deletions relative to amino acids 1064-1076 or amino acids
1105-1114 of SEQ ID NO: 30. In still other aspects of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a Clostridial botulinum serotype F NTNH comprises a
polypeptide having, e.g., at most one, two, three or four
non-contiguous amino acid additions relative to amino acids
1064-1076 or amino acids 1105-1114 of SEQ ID NO: 30. In other
aspects of this embodiment, a .beta.-trefoil domain with enhanced
binding activity derived from a Clostridial botulinum serotype F
NTNH comprises a polypeptide having, e.g., at least one, two, three
or four non-contiguous amino acid additions relative to amino acids
1064-1076 or amino acids 1105-1114 of SEQ ID NO: 30.
[0576] In other aspects of this embodiment, a .beta.-trefoil domain
with enhanced binding activity derived from a Clostridial botulinum
serotype F NTNH comprises a polypeptide having, e.g., at most one,
two, three or four contiguous amino acid substitutions relative to
amino acids 1064-1076 or amino acids 1105-1114 of SEQ ID NO: 30. In
other aspects of this embodiment, a .beta.-trefoil domain with
enhanced binding activity derived from a Clostridial botulinum
serotype F NTNH comprises a polypeptide having, e.g., at least one,
two, three or four contiguous amino acid substitutions relative to
amino acids 1064-1076 or amino acids 1105-1114 of SEQ ID NO: 30. In
other aspects of this embodiment, contiguous amino acid
substitutions of amino acids from amino acids 1064-1076 or amino
acids 1105-1114 of SEQ ID NO: 30 can be replaced with glycine. In
other aspects of this embodiment, contiguous amino acid
substitutions of hydrophobic amino acids from amino acids 1064-1076
or amino acids 1105-1114 of SEQ ID NO: 30 can be replaced with
phenylalanine. In yet other aspects of this embodiment, a
.beta.-trefoil domain with enhanced binding activity derived from a
Clostridial botulinum serotype F NTNH comprises a polypeptide
having, e.g., at most one, two, three or four contiguous amino acid
deletions relative to amino acids 1064-1076 or amino acids
1105-1114 of SEQ ID NO: 30. In other aspects of this embodiment, a
.beta.-trefoil domain with enhanced binding activity derived from a
Clostridial botulinum serotype F NTNH comprises a polypeptide
having, e.g., at least one, two, three or four contiguous amino
acid deletions relative to amino acids 1064-1076 or amino acids
1105-1114 of SEQ ID NO: 30. In still other aspects of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a Clostridial botulinum serotype F NTNH comprises a
polypeptide having, e.g., at most one, two, three or four
contiguous amino acid additions relative to amino acids 1064-1076
or amino acids 1105-1114 of SEQ ID NO: 30. In other aspects of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a Clostridial botulinum serotype F NTNH comprises a
polypeptide having, e.g., at least one, two, three or four
contiguous amino acid additions relative to amino acids 1064-1076
or amino acids 1105-1114 of SEQ ID NO: 30.
[0577] In another embodiment, a .beta.-trefoil domain derived from
a Clostridial botulinum serotype F NTNH comprises a .beta.-trefoil
domain derived from a Clostridial botulinum serotype F NTNH of SEQ
ID NO: 31. In another embodiment, a .beta.-trefoil domain derived
from a Clostridial botulinum serotype F NTNH comprises amino acids
1017-1166 of SEQ ID NO: 31. In another aspect of this embodiment, a
.beta.-trefoil domain derived from a Clostridial botulinum serotype
F NTNH comprises a .alpha.-fold motif of a .beta.-trefoil domain of
a Clostridial botulinum serotype F NTNH, a .beta.-fold motif of a
.beta.-trefoil domain of a Clostridial botulinum serotype F NTNH or
a .gamma.-fold motif of a .beta.-trefoil domain of a Clostridial
botulinum serotype F NTNH of SEQ ID NO: 31. In another aspect of
this embodiment, a .beta.-trefoil domain derived from a Clostridial
botulinum serotype F NTNH comprises amino acids 1017-1064, amino
acids 1078-1106, or amino acids 1117-1166 of SEQ ID NO: 31.
[0578] In another embodiment, a .beta.-trefoil domain with enhanced
binding activity derived from a Clostridial botulinum serotype F
NTNH comprises a .beta.-trefoil domain with enhanced binding
activity derived from a Clostridial botulinum serotype F NTNH of
SEQ ID NO: 31. In another embodiment, a .beta.-trefoil domain with
enhanced binding activity derived from a Clostridial botulinum
serotype F NTNH comprises amino acids 1017-1166 of SEQ ID NO: 31.
In another aspect of this embodiment, a .beta.-trefoil domain with
enhanced binding activity derived from a Clostridial botulinum
serotype F NTNH comprises a modified .alpha.-fold motif of a
.beta.-trefoil domain of a Clostridial botulinum serotype F NTNH, a
modified .beta.-fold motif of a .beta.-trefoil domain of a
Clostridial botulinum serotype F NTNH or a modified .gamma.-fold
motif of a 13-trefoil domain of a Clostridial botulinum serotype F
NTNH of SEQ ID NO: 31. In another aspect of this embodiment, a
.beta.-trefoil domain with enhanced binding activity derived from a
Clostridial botulinum serotype F NTNH comprises amino acids
1017-1064, amino acids 1078-1106, or amino acids 1117-1166 of SEQ
ID NO: 31.
[0579] In other aspects of this embodiment, a Clostridial botulinum
serotype F NTNH comprising a .beta.-trefoil domain with enhanced
binding activity comprises a polypeptide having, e.g., at least 70%
amino acid identity with amino acids 1017-1064, amino acids
1078-1106, or amino acids 1117-1166 of SEQ ID NO: 31, at least 75%
amino acid identity with amino acids 1017-1064, amino acids
1078-1106, or amino acids 1117-1166 of SEQ ID NO: 31, at least 80%
amino acid identity with amino acids 1017-1064, amino acids
1078-1106, or amino acids 1117-1166 of SEQ ID NO: 31, at least 85%
amino acid identity with amino acids 1017-1064, amino acids
1078-1106, or amino acids 1117-1166 of SEQ ID NO: 31, at least 90%
amino acid identity with amino acids 1017-1064, amino acids
1078-1106, or amino acids 1117-1166 of SEQ ID NO: 31 or at least
95% amino acid identity with amino acids 1017-1064, amino acids
1078-1106, or amino acids 1117-1166 of SEQ ID NO: 31. In yet other
aspects of this embodiment, a Clostridial botulinum serotype F NTNH
comprising a .beta.-trefoil domain with enhanced binding activity
comprises a polypeptide having, e.g., at most 70% amino acid
identity with amino acids 1017-1064, amino acids 1078-1106, or
amino acids 1117-1166 of SEQ ID NO: 31, at most 75% amino acid
identity with amino acids 1017-1064, amino acids 1078-1106, or
amino acids 1117-1166 of SEQ ID NO: 31, at most 80% amino acid
identity with amino acids 1017-1064, amino acids 1078-1106, or
amino acids 1117-1166 of SEQ ID NO: 31, at most 85% amino acid
identity with amino acids 1017-1064, amino acids 1078-1106, or
amino acids 1117-1166 of SEQ ID NO: 31, at most 90% amino acid
identity with amino acids 1017-1064, amino acids 1078-1106, or
amino acids 1117-1166 of SEQ ID NO: 31 or at most 95% amino acid
identity with amino acids 1017-1064, amino acids 1078-1106, or
amino acids 1117-1166 of SEQ ID NO: 31.
[0580] In other aspects of this embodiment, a Clostridial botulinum
serotype F NTNH comprising a .beta.-trefoil domain with enhanced
binding activity comprises a polypeptide having, e.g., at most one,
two, three, four, five, six, seven, eight, nine, 10 or 20
non-contiguous amino acid substitutions relative to amino acids
1017-1064, amino acids 1078-1106, or amino acids 1117-1166 of SEQ
ID NO: 31. In other aspects of this embodiment, a Clostridial
botulinum serotype F NTNH comprising a .beta.-trefoil domain with
enhanced binding activity comprises a polypeptide having, e.g., at
least one, two, three, four, five, six, seven, eight, nine, 10 or
20 non-contiguous amino acid substitutions relative to amino acids
1017-1064, amino acids 1078-1106, or amino acids 1117-1166 of SEQ
ID NO: 31. In yet other aspects of this embodiment, a Clostridial
botulinum serotype F NTNH comprising a .beta.-trefoil domain with
enhanced binding activity comprises a polypeptide having, e.g., at
most one, two, three, four, five, six, seven, eight, nine, 10 or 20
non-contiguous amino acid deletions relative to amino acids
1017-1064, amino acids 1078-1106, or amino acids 1117-1166 of SEQ
ID NO: 31. In other aspects of this embodiment, a Clostridial
botulinum serotype F NTNH comprising a .beta.-trefoil domain with
enhanced binding activity comprises a polypeptide having, e.g., at
least one, two, three, four, five, six, seven, eight, nine, 10 or
20 non-contiguous amino acid deletions relative to amino acids
1017-1064, amino acids 1078-1106, or amino acids 1117-1166 of SEQ
ID NO: 31. In still other aspects of this embodiment, a Clostridial
botulinum serotype F NTNH comprising a .beta.-trefoil domain with
enhanced binding activity comprises a polypeptide having, e.g., at
most one, two, three, four, five, six, seven, eight, nine, 10 or 20
non-contiguous amino acid additions relative to amino acids
1017-1064, amino acids 1078-1106, or amino acids 1117-1166 of SEQ
ID NO: 31. In other aspects of this embodiment, a Clostridial
botulinum serotype F NTNH comprising a .beta.-trefoil domain with
enhanced binding activity comprises a polypeptide having, e.g., at
least one, two, three, four, five, six, seven, eight, nine, 10 or
20 non-contiguous amino acid additions relative to amino acids
1017-1064, amino acids 1078-1106, or amino acids 1117-1166 of SEQ
ID NO: 31.
[0581] In other aspects of this embodiment, a Clostridial botulinum
serotype F NTNH comprising a .beta.-trefoil domain with enhanced
binding activity comprises a polypeptide having, e.g., at most one,
two, three, four, five, six, seven, eight, nine, 10 or 20
contiguous amino acid substitutions relative to amino acids
1017-1064, amino acids 1078-1106, or amino acids 1117-1166 of SEQ
ID NO: 31. In other aspects of this embodiment, a Clostridial
botulinum serotype F NTNH comprising a .beta.-trefoil domain with
enhanced binding activity comprises a polypeptide having, e.g., at
least one, two, three, four, five, six, seven, eight, nine, 10 or
20 contiguous amino acid substitutions relative to amino acids
1017-1064, amino acids 1078-1106, or amino acids 1117-1166 of SEQ
ID NO: 31. In yet other aspects of this embodiment, a Clostridial
botulinum serotype F NTNH comprising a .beta.-trefoil domain with
enhanced binding activity comprises a polypeptide having, e.g., at
most one, two, three, four, five, six, seven, eight, nine, 10 or 20
contiguous amino acid deletions relative to amino acids 1017-1064,
amino acids 1078-1106, or amino acids 1117-1166 of SEQ ID NO: 31.
In other aspects of this embodiment, a Clostridial botulinum
serotype F NTNH comprising a .beta.-trefoil domain with enhanced
binding activity comprises a polypeptide having, e.g., at least
one, two, three, four, five, six, seven, eight, nine, 10 or 20
contiguous amino acid deletions relative to amino acids 1017-1064,
amino acids 1078-1106, or amino acids 1117-1166 of SEQ ID NO: 31.
In still other aspects of this embodiment, a Clostridial botulinum
serotype F NTNH comprising a .beta.-trefoil domain with enhanced
binding activity comprises a polypeptide having, e.g., at most one,
two, three, four, five, six, seven, eight, nine, 10 or 20
contiguous amino acid additions relative to amino acids 1017-1064,
amino acids 1078-1106, or amino acids 1117-1166 of SEQ ID NO: 31.
In other aspects of this embodiment, a Clostridial botulinum
serotype F NTNH comprising a .beta.-trefoil domain with enhanced
binding activity comprises a polypeptide having, e.g., at least
one, two, three, four, five, six, seven, eight, nine, 10 or 20
contiguous amino acid additions relative to amino acids 1017-1064,
amino acids 1078-1106, or amino acids 1117-1166 of SEQ ID NO:
31.
[0582] In another embodiment, a .beta.-trefoil domain derived from
a Clostridial botulinum serotype F NTNH comprises a .beta.4/.beta.5
hairpin turn of a .beta.-trefoil domain of a Clostridial botulinum
serotype F NTNH or a 138/139 hairpin turn of a .beta.-trefoil
domain of a Clostridial botulinum serotype F NTNH of SEQ ID NO: 31.
In another aspect of this embodiment, a .beta.-trefoil domain
derived from a Clostridial botulinum serotype F NTNH comprises
amino acids 1065-1077 or amino acids 1107-1116 of SEQ ID NO:
31.
[0583] In another embodiment, a .beta.-trefoil domain with enhanced
binding activity derived from a Clostridial botulinum serotype F
NTNH comprises a modified .beta.4/.beta.5 hairpin turn of a
.beta.-trefoil domain of a Clostridial botulinum serotype F NTNH or
a modified .beta.8/.beta.9 hairpin turn of a .beta.-trefoil domain
of a Clostridial botulinum serotype F NTNH of SEQ ID NO: 31. In
another aspect of this embodiment, a .beta.-trefoil domain with
enhanced binding activity derived from a Clostridial botulinum
serotype F NTNH comprises a modification of amino acids 1065-1077
or amino acids 1107-1116 of SEQ ID NO: 31.
[0584] In other aspects of this embodiment, a .beta.-trefoil domain
with enhanced binding activity derived from a Clostridial botulinum
serotype F NTNH comprises a polypeptide having, e.g., at least 70%
amino acid identity with amino acids 1065-1077 or amino acids
1107-1116 of SEQ ID NO: 31, at least 75% amino acid identity with
amino acids 1065-1077 or amino acids 1107-1116 of SEQ ID NO: 31, at
least 80% amino acid identity with amino acids 1065-1077 or amino
acids 1107-1116 of SEQ ID NO: 31, at least 85% amino acid identity
with amino acids 1065-1077 or amino acids 1107-1116 of SEQ ID NO:
31, at least 90% amino acid identity with amino acids 1065-1077 or
amino acids 1107-1116 of SEQ ID NO: 31 or at least 95% amino acid
identity with amino acids 1065-1077 or amino acids 1107-1116 of SEQ
ID NO: 31. In yet other aspects of this embodiment, a
.beta.-trefoil domain with enhanced binding activity derived from a
Clostridial botulinum serotype F NTNH comprises a polypeptide
having, e.g., at most 70% amino acid identity with amino acids
1065-1077 or amino acids 1107-1116 of SEQ ID NO: 31, at most 75%
amino acid identity with amino acids 1065-1077 or amino acids
1107-1116 of SEQ ID NO: 31, at most 80% amino acid identity with
amino acids 1065-1077 or amino acids 1107-1116 of SEQ ID NO: 31, at
most 85% amino acid identity with amino acids 1065-1077 or amino
acids 1107-1116 of SEQ ID NO: 31, at most 90% amino acid identity
with amino acids 1065-1077 or amino acids 1107-1116 of SEQ ID NO:
31 or at most 95% amino acid identity with amino acids 1065-1077 or
amino acids 1107-1116 of SEQ ID NO: 31.
[0585] In other aspects of this embodiment, a .beta.-trefoil domain
with enhanced binding activity derived from a Clostridial botulinum
serotype F NTNH comprises a polypeptide having, e.g., at most one,
two, three or four non-contiguous amino acid substitutions relative
to amino acids 1065-1077 or amino acids 1107-1116 of SEQ ID NO: 31.
In other aspects of this embodiment, a .beta.-trefoil domain with
enhanced binding activity derived from a Clostridial botulinum
serotype F NTNH comprises a polypeptide having, e.g., at least one,
two, three or four non-contiguous amino acid substitutions relative
to amino acids 1065-1077 or amino acids 1107-1116 of SEQ ID NO: 31.
In other aspects of this embodiment, a non-contiguous amino acid
substitution of any amino acid from amino acids 1065-1077 or amino
acids 1107-1116 of SEQ ID NO: 31 can be replaced with glycine. In
other aspects of this embodiment, a non-contiguous amino acid
substitution of any hydrophobic amino acid from amino acids
1065-1077 or amino acids 1107-1116 of SEQ ID NO: 31 can be replaced
with phenylalanine. In yet other aspects of this embodiment, a
.beta.-trefoil domain with enhanced binding activity derived from a
Clostridial botulinum serotype F NTNH comprises a polypeptide
having, e.g., at most one, two, three or four non-contiguous amino
acid deletions relative to amino acids 1065-1077 or amino acids
1107-1116 of SEQ ID NO: 31. In other aspects of this embodiment, a
.beta.-trefoil domain with enhanced binding activity derived from a
Clostridial botulinum serotype F NTNH comprises a polypeptide
having, e.g., at least one, two, three or four non-contiguous amino
acid deletions relative to amino acids 1065-1077 or amino acids
1107-1116 of SEQ ID NO: 31. In still other aspects of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a Clostridial botulinum serotype F NTNH comprises a
polypeptide having, e.g., at most one, two, three or four
non-contiguous amino acid additions relative to amino acids
1065-1077 or amino acids 1107-1116 of SEQ ID NO: 31. In other
aspects of this embodiment, a .beta.-trefoil domain with enhanced
binding activity derived from a Clostridial botulinum serotype F
NTNH comprises a polypeptide having, e.g., at least one, two, three
or four non-contiguous amino acid additions relative to amino acids
1065-1077 or amino acids 1107-1116 of SEQ ID NO: 31.
[0586] In other aspects of this embodiment, a .beta.-trefoil domain
with enhanced binding activity derived from a Clostridial botulinum
serotype F NTNH comprises a polypeptide having, e.g., at most one,
two, three or four contiguous amino acid substitutions relative to
amino acids 1065-1077 or amino acids 1107-1116 of SEQ ID NO: 31. In
other aspects of this embodiment, a .beta.-trefoil domain with
enhanced binding activity derived from a Clostridial botulinum
serotype F NTNH comprises a polypeptide having, e.g., at least one,
two, three or four contiguous amino acid substitutions relative to
amino acids 1065-1077 or amino acids 1107-1116 of SEQ ID NO: 31. In
other aspects of this embodiment, contiguous amino acid
substitutions of amino acids from amino acids 1065-1077 or amino
acids 1107-1116 of SEQ ID NO: 31 can be replaced with glycine. In
other aspects of this embodiment, contiguous amino acid
substitutions of hydrophobic amino acids from amino acids 1065-1077
or amino acids 1107-1116 of SEQ ID NO: 31 can be replaced with
phenylalanine. In yet other aspects of this embodiment, a
.beta.-trefoil domain with enhanced binding activity derived from a
Clostridial botulinum serotype F NTNH comprises a polypeptide
having, e.g., at most one, two, three or four contiguous amino acid
deletions relative to amino acids 1065-1077 or amino acids
1107-1116 of SEQ ID NO: 31. In other aspects of this embodiment, a
.beta.-trefoil domain with enhanced binding activity derived from a
Clostridial botulinum serotype F NTNH comprises a polypeptide
having, e.g., at least one, two, three or four contiguous amino
acid deletions relative to amino acids 1065-1077 or amino acids
1107-1116 of SEQ ID NO: 31. In still other aspects of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a Clostridial botulinum serotype F NTNH comprises a
polypeptide having, e.g., at most one, two, three or four
contiguous amino acid additions relative to amino acids 1065-1077
or amino acids 1107-1116 of SEQ ID NO: 31. In other aspects of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a Clostridial botulinum serotype F NTNH comprises a
polypeptide having, e.g., at least one, two, three or four
contiguous amino acid additions relative to amino acids 1065-1077
or amino acids 1107-1116 of SEQ ID NO: 31.
[0587] In another embodiment, a .beta.-trefoil domain derived from
a Clostridial botulinum serotype G NTNH comprises a .beta.-trefoil
domain derived from a Clostridial botulinum serotype G NTNH of SEQ
ID NO: 32. In another embodiment, a .beta.-trefoil domain derived
from a Clostridial botulinum serotype G NTNH comprises amino acids
1050-1199 of SEQ ID NO: 32. In another aspect of this embodiment, a
.beta.-trefoil domain derived from a Clostridial botulinum serotype
G NTNH comprises a .alpha.-fold motif of a .beta.-trefoil domain of
a Clostridial botulinum serotype G NTNH, a .beta.-fold motif of a
.beta.-trefoil domain of a Clostridial botulinum serotype G NTNH or
a .gamma.-fold motif of a .beta.-trefoil domain of a Clostridial
botulinum serotype G NTNH of SEQ ID NO: 32. In another aspect of
this embodiment, a .beta.-trefoil domain derived from a Clostridial
botulinum serotype G NTNH comprises amino acids 1050-1097, amino
acids 1111-1139, or amino acids 1150-1199 of SEQ ID NO: 32.
[0588] In another embodiment, a .beta.-trefoil domain with enhanced
binding activity derived from a Clostridial botulinum serotype G
NTNH comprises a .beta.-trefoil domain with enhanced binding
activity derived from a Clostridial botulinum serotype G NTNH of
SEQ ID NO: 32. In another embodiment, a .beta.-trefoil domain with
enhanced binding activity derived from a Clostridial botulinum
serotype G NTNH comprises amino acids 1050-1199 of SEQ ID NO: 32.
In another aspect of this embodiment, a .beta.-trefoil domain with
enhanced binding activity derived from a Clostridial botulinum
serotype G NTNH comprises a modified .alpha.-fold motif of a
.beta.-trefoil domain of a Clostridial botulinum serotype G NTNH, a
modified .beta.-fold motif of a .beta.-trefoil domain of a
Clostridial botulinum serotype G NTNH or a modified .gamma.-fold
motif of a .beta.-trefoil domain of a Clostridial botulinum
serotype G NTNH of SEQ ID NO: 32. In another aspect of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a Clostridial botulinum serotype G NTNH comprises
amino acids 1050-1097, amino acids 1111-1139, or amino acids
1150-1199 of SEQ ID NO: 32.
[0589] In other aspects of this embodiment, a Clostridial botulinum
serotype G NTNH comprising a .beta.-trefoil domain with enhanced
binding activity comprises a polypeptide having, e.g., at least 70%
amino acid identity with amino acids 1050-1097, amino acids
1111-1139, or amino acids 1150-1199 of SEQ ID NO: 32, at least 75%
amino acid identity with amino acids 1050-1097, amino acids
1111-1139, or amino acids 1150-1199 of SEQ ID NO: 32, at least 80%
amino acid identity with amino acids 1050-1097, amino acids
1111-1139, or amino acids 1150-1199 of SEQ ID NO: 32, at least 85%
amino acid identity with amino acids 1050-1097, amino acids
1111-1139, or amino acids 1150-1199 of SEQ ID NO: 32, at least 90%
amino acid identity with amino acids 1050-1097, amino acids
1111-1139, or amino acids 1150-1199 of SEQ ID NO: 32 or at least
95% amino acid identity with amino acids 1050-1097, amino acids
1111-1139, or amino acids 1150-1199 of SEQ ID NO: 32. In yet other
aspects of this embodiment, a Clostridial botulinum serotype G NTNH
comprising a .beta.-trefoil domain with enhanced binding activity
comprises a polypeptide having, e.g., at most 70% amino acid
identity with amino acids 1050-1097, amino acids 1111-1139, or
amino acids 1150-1199 of SEQ ID NO: 32, at most 75% amino acid
identity with amino acids 1050-1097, amino acids 1111-1139, or
amino acids 1150-1199 of SEQ ID NO: 32, at most 80% amino acid
identity with amino acids 1050-1097, amino acids 1111-1139, or
amino acids 1150-1199 of SEQ ID NO: 32, at most 85% amino acid
identity with amino acids 1050-1097, amino acids 1111-1139, or
amino acids 1150-1199 of SEQ ID NO: 32, at most 90% amino acid
identity with amino acids 1050-1097, amino acids 1111-1139, or
amino acids 1150-1199 of SEQ ID NO: 32 or at most 95% amino acid
identity with amino acids 1050-1097, amino acids 1111-1139, or
amino acids 1150-1199 of SEQ ID NO: 32.
[0590] In other aspects of this embodiment, a Clostridial botulinum
serotype G NTNH comprising a .beta.-trefoil domain with enhanced
binding activity comprises a polypeptide having, e.g., at most one,
two, three, four, five, six, seven, eight, nine, 10 or 20
non-contiguous amino acid substitutions relative to amino acids
1050-1097, amino acids 1111-1139, or amino acids 1150-1199 of SEQ
ID NO: 32. In other aspects of this embodiment, a Clostridial
botulinum serotype G NTNH comprising a .beta.-trefoil domain with
enhanced binding activity comprises a polypeptide having, e.g., at
least one, two, three, four, five, six, seven, eight, nine, 10 or
20 non-contiguous amino acid substitutions relative to amino acids
1050-1097, amino acids 1111-1139, or amino acids 1150-1199 of SEQ
ID NO: 32. In yet other aspects of this embodiment, a Clostridial
botulinum serotype G NTNH comprising a .beta.-trefoil domain with
enhanced binding activity comprises a polypeptide having, e.g., at
most one, two, three, four, five, six, seven, eight, nine, 10 or 20
non-contiguous amino acid deletions relative to amino acids
1050-1097, amino acids 1111-1139, or amino acids 1150-1199 of SEQ
ID NO: 32. In other aspects of this embodiment, a Clostridial
botulinum serotype G NTNH comprising a .beta.-trefoil domain with
enhanced binding activity comprises a polypeptide having, e.g., at
least one, two, three, four, five, six, seven, eight, nine, 10 or
20 non-contiguous amino acid deletions relative to amino acids
1050-1097, amino acids 1111-1139, or amino acids 1150-1199 of SEQ
ID NO: 32. In still other aspects of this embodiment, a Clostridial
botulinum serotype G NTNH comprising a .beta.-trefoil domain with
enhanced binding activity comprises a polypeptide having, e.g., at
most one, two, three, four, five, six, seven, eight, nine, 10 or 20
non-contiguous amino acid additions relative to amino acids
1050-1097, amino acids 1111-1139, or amino acids 1150-1199 of SEQ
ID NO: 32. In other aspects of this embodiment, a Clostridial
botulinum serotype G NTNH comprising a .beta.-trefoil domain with
enhanced binding activity comprises a polypeptide having, e.g., at
least one, two, three, four, five, six, seven, eight, nine, 10 or
20 non-contiguous amino acid additions relative to amino acids
1050-1097, amino acids 1111-1139, or amino acids 1150-1199 of SEQ
ID NO: 32.
[0591] In other aspects of this embodiment, a Clostridial botulinum
serotype G NTNH comprising a .beta.-trefoil domain with enhanced
binding activity comprises a polypeptide having, e.g., at most one,
two, three, four, five, six, seven, eight, nine, 10 or 20
contiguous amino acid substitutions relative to amino acids
1050-1097, amino acids 1111-1139, or amino acids 1150-1199 of SEQ
ID NO: 32. In other aspects of this embodiment, a Clostridial
botulinum serotype G NTNH comprising a .beta.-trefoil domain with
enhanced binding activity comprises a polypeptide having, e.g., at
least one, two, three, four, five, six, seven, eight, nine, 10 or
20 contiguous amino acid substitutions relative to amino acids
1050-1097, amino acids 1111-1139, or amino acids 1150-1199 of SEQ
ID NO: 32. In yet other aspects of this embodiment, a Clostridial
botulinum serotype G NTNH comprising a .beta.-trefoil domain with
enhanced binding activity comprises a polypeptide having, e.g., at
most one, two, three, four, five, six, seven, eight, nine, 10 or 20
contiguous amino acid deletions relative to amino acids 1050-1097,
amino acids 1111-1139, or amino acids 1150-1199 of SEQ ID NO: 32.
In other aspects of this embodiment, a Clostridial botulinum
serotype G NTNH comprising a .beta.-trefoil domain with enhanced
binding activity comprises a polypeptide having, e.g., at least
one, two, three, four, five, six, seven, eight, nine, 10 or 20
contiguous amino acid deletions relative to amino acids 1050-1097,
amino acids 1111-1139, or amino acids 1150-1199 of SEQ ID NO: 32.
In still other aspects of this embodiment, a Clostridial botulinum
serotype G NTNH comprising a .beta.-trefoil domain with enhanced
binding activity comprises a polypeptide having, e.g., at most one,
two, three, four, five, six, seven, eight, nine, 10 or 20
contiguous amino acid additions relative to amino acids 1050-1097,
amino acids 1111-1139, or amino acids 1150-1199 of SEQ ID NO: 32.
In other aspects of this embodiment, a Clostridial botulinum
serotype G NTNH comprising a .beta.-trefoil domain with enhanced
binding activity comprises a polypeptide having, e.g., at least
one, two, three, four, five, six, seven, eight, nine, 10 or 20
contiguous amino acid additions relative to amino acids 1050-1097,
amino acids 1111-1139, or amino acids 1150-1199 of SEQ ID NO:
32.
[0592] In another embodiment, a .beta.-trefoil domain derived from
a Clostridial botulinum serotype G NTNH comprises a .beta.4/.beta.5
hairpin turn of a .beta.-trefoil domain of a Clostridial botulinum
serotype G NTNH or a .beta.8/.beta.9 hairpin turn of a
.beta.-trefoil domain of a Clostridial botulinum serotype G NTNH of
SEQ ID NO: 32. In another aspect of this embodiment, a
.beta.-trefoil domain derived from a Clostridial botulinum serotype
G NTNH comprises amino acids 1098-1110 or amino acids 1140-1149 of
SEQ ID NO: 32.
[0593] In another embodiment, a .beta.-trefoil domain with enhanced
binding activity derived from a Clostridial botulinum serotype G
NTNH comprises a modified .beta.4/.beta.5 hairpin turn of a
.beta.-trefoil domain of a Clostridial botulinum serotype G NTNH or
a modified .beta.8/.beta.9 hairpin turn of a .beta.-trefoil domain
of a Clostridial botulinum serotype G NTNH of SEQ ID NO: 32. In
another aspect of this embodiment, a .beta.-trefoil domain with
enhanced binding activity derived from a Clostridial botulinum
serotype G NTNH comprises a modification of amino acids 1098-1110
or amino acids 1140-1149 of SEQ ID NO: 32.
[0594] In other aspects of this embodiment, a .beta.-trefoil domain
with enhanced binding activity derived from a Clostridial botulinum
serotype G NTNH comprises a polypeptide having, e.g., at least 70%
amino acid identity with amino acids 1098-1110 or amino acids
1140-1149 of SEQ ID NO: 32, at least 75% amino acid identity with
amino acids 1098-1110 or amino acids 1140-1149 of SEQ ID NO: 32, at
least 80% amino acid identity with amino acids 1098-1110 or amino
acids 1140-1149 of SEQ ID NO: 32, at least 85% amino acid identity
with amino acids 1098-1110 or amino acids 1140-1149 of SEQ ID NO:
32, at least 90% amino acid identity with amino acids 1098-1110 or
amino acids 1140-1149 of SEQ ID NO: 32 or at least 95% amino acid
identity with amino acids 1098-1110 or amino acids 1140-1149 of SEQ
ID NO: 32. In yet other aspects of this embodiment, a
.beta.-trefoil domain with enhanced binding activity derived from a
Clostridial botulinum serotype G NTNH comprises a polypeptide
having, e.g., at most 70% amino acid identity with amino acids
1098-1110 or amino acids 1140-1149 of SEQ ID NO: 32, at most 75%
amino acid identity with amino acids 1098-1110 or amino acids
1140-1149 of SEQ ID NO: 32, at most 80% amino acid identity with
amino acids 1098-1110 or amino acids 1140-1149 of SEQ ID NO: 32, at
most 85% amino acid identity with amino acids 1098-1110 or amino
acids 1140-1149 of SEQ ID NO: 32, at most 90% amino acid identity
with amino acids 1098-1110 or amino acids 1140-1149 of SEQ ID NO:
32 or at most 95% amino acid identity with amino acids 1098-1110 or
amino acids 1140-1149 of SEQ ID NO: 32.
[0595] In other aspects of this embodiment, a .beta.-trefoil domain
with enhanced binding activity derived from a Clostridial botulinum
serotype G NTNH comprises a polypeptide having, e.g., at most one,
two, three or four non-contiguous amino acid substitutions relative
to amino acids 1098-1110 or amino acids 1140-1149 of SEQ ID NO: 32.
In other aspects of this embodiment, a .beta.-trefoil domain with
enhanced binding activity derived from a Clostridial botulinum
serotype G NTNH comprises a polypeptide having, e.g., at least one,
two, three or four non-contiguous amino acid substitutions relative
to amino acids 1098-1110 or amino acids 1140-1149 of SEQ ID NO: 32.
In other aspects of this embodiment, a non-contiguous amino acid
substitution of any amino acid from amino acids 1098-1110 or amino
acids 1140-1149 of SEQ ID NO: 32 can be replaced with glycine. In
other aspects of this embodiment, a non-contiguous amino acid
substitution of any hydrophobic amino acid from amino acids
1098-1110 or amino acids 1140-1149 of SEQ ID NO: 32 can be replaced
with phenylalanine. In yet other aspects of this embodiment, a
.beta.-trefoil domain with enhanced binding activity derived from a
Clostridial botulinum serotype G NTNH comprises a polypeptide
having, e.g., at most one, two, three or four non-contiguous amino
acid deletions relative to amino acids 1098-1110 or amino acids
1140-1149 of SEQ ID NO: 32. In other aspects of this embodiment, a
.beta.-trefoil domain with enhanced binding activity derived from a
Clostridial botulinum serotype G NTNH comprises a polypeptide
having, e.g., at least one, two, three or four non-contiguous amino
acid deletions relative to amino acids 1098-1110 or amino acids
1140-1149 of SEQ ID NO: 32. In still other aspects of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a Clostridial botulinum serotype G NTNH comprises a
polypeptide having, e.g., at most one, two, three or four
non-contiguous amino acid additions relative to amino acids
1098-1110 or amino acids 1140-1149 of SEQ ID NO: 32. In other
aspects of this embodiment, a .beta.-trefoil domain with enhanced
binding activity derived from a Clostridial botulinum serotype G
NTNH comprises a polypeptide having, e.g., at least one, two, three
or four non-contiguous amino acid additions relative to amino acids
1098-1110 or amino acids 1140-1149 of SEQ ID NO: 32.
[0596] In other aspects of this embodiment, a .beta.-trefoil domain
with enhanced binding activity derived from a Clostridial botulinum
serotype G NTNH comprises a polypeptide having, e.g., at most one,
two, three or four contiguous amino acid substitutions relative to
amino acids 1098-1110 or amino acids 1140-1149 of SEQ ID NO: 32. In
other aspects of this embodiment, a .beta.-trefoil domain with
enhanced binding activity derived from a Clostridial botulinum
serotype G NTNH comprises a polypeptide having, e.g., at least one,
two, three or four contiguous amino acid substitutions relative to
amino acids 1098-1110 or amino acids 1140-1149 of SEQ ID NO: 32. In
other aspects of this embodiment, contiguous amino acid
substitutions of amino acids from amino acids 1098-1110 or amino
acids 1140-1149 of SEQ ID NO: 32 can be replaced with glycine. In
other aspects of this embodiment, contiguous amino acid
substitutions of hydrophobic amino acids from amino acids 1098-1110
or amino acids 1140-1149 of SEQ ID NO: 32 can be replaced with
phenylalanine. In yet other aspects of this embodiment, a
.beta.-trefoil domain with enhanced binding activity derived from a
Clostridial botulinum serotype G NTNH comprises a polypeptide
having, e.g., at most one, two, three or four contiguous amino acid
deletions relative to amino acids 1098-1110 or amino acids
1140-1149 of SEQ ID NO: 32. In other aspects of this embodiment, a
.beta.-trefoil domain with enhanced binding activity derived from a
Clostridial botulinum serotype G NTNH comprises a polypeptide
having, e.g., at least one, two, three or four contiguous amino
acid deletions relative to amino acids 1098-1110 or amino acids
1140-1149 of SEQ ID NO: 32. In still other aspects of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a Clostridial botulinum serotype G NTNH comprises a
polypeptide having, e.g., at most one, two, three or four
contiguous amino acid additions relative to amino acids 1098-1110
or amino acids 1140-1149 of SEQ ID NO: 32. In other aspects of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a Clostridial botulinum serotype G NTNH comprises a
polypeptide having, e.g., at least one, two, three or four
contiguous amino acid additions relative to amino acids 1098-1110
or amino acids 1140-1149 of SEQ ID NO: 32.
[0597] Another example of an enhanced targeting domain that
increases binding activity for an endogenous Clostridial toxin
receptor present on a naturally-occurring Clostridial toxin target
cell, includes, without limitation, ligands that bind the same
receptors as naturally-occurring Clostridial toxins. Recent studies
are revealing components of the endogenous receptors used by a
Clostridial toxin during the intoxication process. As a
non-limiting example, fibroblast growth factor 3 receptor (FGFR3)
serves as a BoNT/A receptor, see, e.g., Ester Fernandez-Salas et
al., Botulinum Toxin Screening Assays, PCT Patent Application No.
2005/006421 (Sep., 9, 2005). As another non-limiting example,
synaptotagmin I serves as a BoNT/B receptor and as a BoNT/G
receptor, see, e.g., Min Dong et al., Synaptotagmins I and II
mediate entry of botulinum neurotoxin B into cells, 162(7) J. Cell
Biol. 1293-1303 (2003); and Andreas Rummel et al., Synaptotagmins I
and II act as nerve cell receptors for botulinum neurotoxin G,
279(29) J. Biol. Chem. 30865-30870 (2004). As yet another
non-limiting example, synaptotagmin II serves as a BoNT/B receptor
and as a BoNT/G receptor, see, e.g., Min Dong et al., supra,
(2003); and Andreas Rummel et al., supra, (2004). The selection of
a ligand domain as an enhanced binding region will depend on the
Clostridial toxin being modified. As non-limiting examples, ligands
that selectively bind to a FGFR3 could be used as an enhanced
targeting domain for a modified BoNT/A, whereas ligands that
selectively bind a Synaptotagmin I or Synaptotagmin II could be
used as an enhanced targeting domain for a modified BoNT/B or a
modified BoNT/G.
[0598] In addition to its enhanced targeting activity, replacement
of a naturally-occurring targeting domain with an FGF ligand has an
added advantage of reducing the likelihood of the modified toxin
from eliciting an immunogenic response. Regions found in the
H.sub.CC targeting domain are bound by neutralizing anti-BoNT/A
antibodies, see, e.g., M. Zouhair Atassi et al., Mapping of the
Antibody-binding Regions on Botulinum Neurotoxin H-chain Domain
855-1296 with Anti-toxin Antibodies from Three Host Species, 15 J.
PROT. CHEM. 691-700, (1996); M. Zouhair Atassi & Behzod Z.
Dolimbek, Mapping of the Antibody-binding Profile on Botulinum
Neurotoxin A H.sub.N-domain (residues 449-859) with Anti-toxin
Antibodies from Four Host Species. Antigenic Profile of the Entire
H-chain of Botulinum Neurotoxin A, 23(1) PROTEIN J. 39-52, (2004).
Therefore, elimination of this targeting domain will reduce the
likelihood of an immunogenic response because 1) the Clostridial
toxin H.sub.CC targeting domain is absent; 2) a human FGF targeting
domain will most likely not elicit an immunogenic response in a
patient because it is a human polypeptide.
[0599] Fibroblast growth factors (FGF) participate in many
developmental, differentiation and growth and repair processes of
cells through complex combinatorial signaling pathways. Presently,
at least 23 ligands (FGF1-23) are known to signal through a family
of five transmembrane tyrosine kinase FGF receptors (FGFR1-5).
Affinity of FGFRs for their ligands is highly diverse with
different affinities for each family member of growth factors, see,
e.g., C. J. Powers et al., Fibroblast growth factors, their
receptors and signaling, 7.beta.) Endocr. Relat. Cancer. 165-197
(2000). This diversity is achieved in part by the generation of
alternatively spliced variants encoding distinct receptor isoforms,
see, e.g., Bernhard Reuss & Oliver von Bohlen and Halbach,
Fibroblast growth factors and their receptors in the central
nervous system, 313(2) Cell Tissue Res. 139-157 (2003). The protein
region that appears to have the highest influence on ligand binding
selectivity is a portion of the 1011 domain, for which isoforms
encoded by three different splice variants have been identified.
These three isoforms, designated IgIIIa, IgIIIb and IgIIIc, have
relative binding affinities for different FGFR family members.
Alternative splicing in the FGFR ligand binding domain, designated
a and b, generates additional receptor isoforms with novel ligand
affinities. Isoforms for IgIIIa, IgIIIb and IgIIIc have been
identified for both FGFR1 and FGFR2. Thus far, the IgIIIa isoform
of FGFR3 and the IgIIIa and IgIIIb isoforms of FGFR4 and FGFR5 have
not been reported.
[0600] Currently, several FGFs have been shown to selectively bind
FGFR3, such as, e.g., FGF-1, FGF-2, FGF-4, FGF-8, FGF-9, FGF-17 and
FGF-18, see, e.g., Hecht et al., 1995; Ornitz et al., 1996; and Xu
et al., 2000. Additional studies have revealed that FGF-1 and FGF9
preferentially bind FGFR3IIIb, whereas FGF-1 FGF-2, FGF-4, FGF-8,
and FGF9 preferentially bind FGFR3IIIc. Studies have shown that
each of these ligands is present in various vertebrate species with
a high degree of sequence identity which suggests functional
equivalence or similarity. As a non-limiting example, FGF-8 is
found in fish, birds and mammals and each of these FGF-8 ligands
have over 80% amino acid sequence identity. As another non-limiting
example, FGF-18 is found in fish, birds and mammals and each of
these FGF-18 ligands have over 70% amino acid sequence identity.
Crystallographic studies have revealed that all FGFs are
structurally organized into a .beta.-trefoil domain. The amino acid
sequences comprising the .beta.-trefoil domains found in various
FGF ligands that bind FGFR3 are shown in Table 7.
TABLE-US-00007 TABLE 7 .beta.-trefoil Domains of FGFs Amino Acid
Sequence Region of Carbohydrate Binding Moieties .beta.4/.beta.5
.beta.8/.beta.9 Ligand SEQ ID NO: .alpha.-fold .beta.-hairpin turn
.beta.-fold .beta.-hairpin turn .gamma.-fold FGF-1 33 26-64 65-67
68-105 106-108 109-155 FGF-2 34 29-67 68-70 71-111 112-114 115-155
FGF-4 35 83-121 122-124 125-162 163-165 166-206 FGF-8 36 43-80
81-83 84-123 124-126 127-172 FGF-9 37 63-100 101-103 104-144
145-147 148-196 FGF-17 38 55-91 92-94 95-134 135-137 138-183 FGF-18
39 54-91 92-94 95-134 135-137 138-183
[0601] As used herein, the term "Fibroblast Growth Factor" is
synonymous with "FGF" and means a polypeptide with selective
binding activity, such as, e.g., a binding affinity or a binding
specificity, for a receptor, including endogenous Clostridial toxin
receptors such as, e.g., a receptor comprising FGFR3. It is
envisioned that both naturally occurring FGFs as well as FGFs with
enhanced binding activity can be used to practice aspects of the
present invention. As used herein, the term "FGF with enhanced
binding activity" means a FGF with enhanced binding activity for an
endogenous Clostridial toxin receptor, such as, e.g., a binding
affinity or a binding specificity, to a statistically significantly
degree relative to an unmodified naturally occurring Clostridial
toxin binding domain from a Clostridial toxin. By definition, a FGF
with enhanced binding activity has at least one amino acid change
from the corresponding region of the disclosed reference sequences
(see Table 7) and can be described in percent identity to the
corresponding region of that reference sequence.
[0602] Any of a variety of sequence alignment methods can be used
to determine percent identity of a modified FGF relative to a
naturally-occurring FGF, including, without limitation, global
methods, local methods and hybrid methods, such as, e.g., segment
approach methods. Protocols to determine percent identity are
routine procedures within the scope of one skilled in the art and
from the teaching herein.
[0603] Approaches well known to one skilled in the art on how to
modify a FGF in order to increase its binding activity for an
endogenous Clostridial toxin receptor present on a
naturally-occurring Clostridial toxin target cell. As described
above, one approach involves identifying amino acids using
computational protein design algorithms; changing
specifically-identified amino acids using, without limitation,
site-directed mutagenesis, oligonucleotide-directed mutagenesis and
site-specific mutagenesis; and testing the binding activity of
modified Clostridial toxins comprising a modified FGF with enhanced
binding activity using, e.g., heterogeneous assays, homogeneous
assays and non-separating homogeneous assays. It is further
envisioned that the binding activity of a modified Clostridial
toxin with enhanced binding activity disclosed in the present
specification can be determined by affinity chromatography using
immobilized receptors and interfacial optical assays. In another
approach described above, a binding activity of a modified FGF for
a naturally-occurring Clostridial toxin receptor present on a
naturally-occurring Clostridial toxin target cell can be achieved
using directed-evolution methods.
[0604] A FGF includes, without limitation, naturally occurring FGF
variants, such as, e.g., FGF isoforms and FGF subtypes;
non-naturally occurring FGF variants, such as, e.g., conservative
FGF variants, non-conservative FGF variants, FGF chimerics, active
FGF fragments thereof, or any combination thereof.
[0605] As used herein, the term "FGF variant," whether
naturally-occurring or non-naturally-occurring, means a FGF that
has at least one amino acid change from the corresponding region of
the disclosed reference sequences (see Tables 7) and can be
described in percent identity to the corresponding region of that
reference sequence. Unless expressly indicated, all FGF variants
disclosed in the present specification are capable of executing the
cell binding step of the intoxication process.
[0606] It is recognized by those of skill in the art that within
each Clostridial bacterium there can be naturally occurring FGF
variants that differ somewhat in their amino acid sequence, and
also in the nucleic acids encoding these proteins. For example,
there are presently at least four FGF-8 variants, FGF-8A, FGF-8A,
FGF-8B, FGF-8E and FGF-8F, with specific FGF-8 variants showing
various degrees of amino acid divergence when compared to another
FGF-8 variant. As another example, there are presently at least two
FGF-2 variants, FGF-2-1 and FGF-2-1, with the FGF-2-1 variant
showing an amino acid divergence when compared to the FGF-2-2
variant. As used herein, the term "naturally occurring FGF variant"
means any FGF produced by a naturally-occurring process, including,
without limitation, FGF isoforms produced from
alternatively-spliced transcripts, FGF isoforms produced by
spontaneous mutation and FGF subtypes. A naturally occurring FGF
variant can function in substantially the same manner as the
reference FGF on which the naturally occurring FGF variant is
based, and can be substituted for the reference FGF in any aspect
of the present invention. A naturally occurring FGF variant may
substitute one or more amino acids, two or more amino acids, three
or more amino acids, four or more amino acids, five or more amino
acids, ten or more amino acids, 20 or more amino acids, 30 or more
amino acids, 40 or more amino acids, 50 or more amino acids or 100
or more amino acids from the reference FGF on which the naturally
occurring FGF variant is based. A naturally occurring FGF variant
can also substitute at least 10 contiguous amino acids, at least 15
contiguous amino acids, at least 20 contiguous amino acids, or at
least 25 contiguous amino acids from the reference FGF on which the
naturally occurring FGF variant is based, that possess at least 50%
amino acid identity, 65% amino acid identity, 75% amino acid
identity, 85% amino acid identity or 95% amino acid identity to the
reference FGF on which the naturally occurring FGF variant is
based.
[0607] A non-limiting examples of a naturally occurring FGF variant
is a FGF isoform such as, e.g., a FGF-1 isoform, a FGF-2 isoform, a
FGF-4 isoform, a FGF-8 isoform, a FGF-9 isoform, a FGF-17 isoform
and a FGF-18 isoform. A FGF isoform can function in substantially
the same manner as the reference FGF on which the FGF isoform is
based, and can be substituted for the reference FGF in any aspect
of the present invention.
[0608] Another non-limiting examples of a naturally occurring FGF
variant is a FGF subtype such as, e.g., a FGF-1 subtype, a FGF-2
subtype, a FGF-4 subtype, a FGF-8 subtype, a FGF-9 subtype, a
FGF-17 subtype and a FGF-18 subtype. A FGF subtype can function in
substantially the same manner as the reference FGF on which the FGF
subtype is based, and can be substituted for the reference FGF in
any aspect of the present invention.
[0609] As used herein, the term "non-naturally occurring FGF
variant" means any FGF produced with the aid of human manipulation,
including, without limitation, FGFs produced by genetic engineering
using random mutagenesis or rational design and FGFs produced by
chemical synthesis. Non-limiting examples of non-naturally
occurring FGF variants include, e.g., conservative FGF variants,
non-conservative FGF variants, FGF chimeric variants and active FGF
fragments.
[0610] As used herein, the term "conservative FGF variant" means a
FGF that has at least one amino acid substituted by another amino
acid or an amino acid analog that has at least one property similar
to that of the original amino acid from the reference FGF sequence
(see Table 7). Examples of properties include, without limitation,
similar size, topography, charge, hydrophobicity, hydrophilicity,
lipophilicity, covalent-bonding capacity, hydrogen-bonding
capacity, a physicochemical property, of the like, or any
combination thereof. A conservative FGF variant can function in
substantially the same manner as the reference FGF on which the
conservative FGF variant is based, and can be substituted for the
reference FGF in any aspect of the present invention. A
conservative FGF variant may substitute one or more amino acids,
two or more amino acids, three or more amino acids, four or more
amino acids, five or more amino acids, ten or more amino acids, 20
or more amino acids, 30 or more amino acids, 40 or more amino acids
or 50 or more amino acids from the reference FGF on which the
conservative FGF variant is based. A conservative FGF variant can
also substitute at least 10 contiguous amino acids, at least 15
contiguous amino acids, at least 20 contiguous amino acids, or at
least 25 contiguous amino acids from the reference FGF on which the
conservative FGF variant is based, that possess at least 50% amino
acid identity, 65% amino acid identity, 75% amino acid identity,
85% amino acid identity or 95% amino acid identity to the reference
FGF on which the conservative FGF variant is based. Non-limiting
examples of a conservative FGF variant include, e.g., a
conservative FGF-1 variant, a conservative FGF-2 variant, a
conservative FGF-4 variant, a conservative FGF-8 variant, a
conservative FGF-9 variant, a conservative FGF-17 variant and a
conservative FGF-18 variant.
[0611] As used herein, the term "non-conservative FGF variant"
means a FGF in which 1) at least one amino acid is deleted from the
reference FGF on which the non-conservative FGF variant is based;
2) at least one amino acid added to the reference FGF on which the
non-conservative FGF is based; or 3) at least one amino acid is
substituted by another amino acid or an amino acid analog that does
not share any property similar to that of the original amino acid
from the reference FGF sequence (see Table 7). A non-conservative
FGF variant can function in substantially the same manner as the
reference FGF on which the non-conservative FGF variant is based,
and can be substituted for the reference FGF in any aspect of the
present invention. A non-conservative FGF variant can delete one or
more amino acids, two or more amino acids, three or more amino
acids, four or more amino acids, five or more amino acids, and ten
or more amino acids from the reference FGF on which the
non-conservative FGF variant is based. A non-conservative FGF
variant can add one or more amino acids, two or more amino acids,
three or more amino acids, four or more amino acids, five or more
amino acids, and ten or more amino acids to the reference FGF on
which the non-conservative FGF variant is based. A non-conservative
FGF variant may substitute one or more amino acids, two or more
amino acids, three or more amino acids, four or more amino acids,
five or more amino acids, ten or more amino acids, 20 or more amino
acids, 30 or more amino acids, 40 or more amino acids or 50 or more
amino acids from the reference FGF on which the non-conservative
FGF variant is based. A non-conservative FGF variant can also
substitute at least 10 contiguous amino acids, at least 15
contiguous amino acids, at least 20 contiguous amino acids, or at
least 25 contiguous amino acids from the reference FGF on which the
non-conservative FGF variant is based, that possess at least 50%
amino acid identity, 65% amino acid identity, 75% amino acid
identity, 85% amino acid identity or 95% amino acid identity to the
reference FGF on which the non-conservative FGF variant is based.
Non-limiting examples of a non-conservative FGF variant include,
e.g., a non-conservative FGF-1 variant, a non-conservative FGF-2
variant, a non-conservative FGF-4 variant, a non-conservative FGF-8
variant, a non-conservative FGF-9 variant, a non-conservative
FGF-17 variant and a non-conservative FGF-18 variant.
[0612] As used herein, the term "FGF chimeric" means a polypeptide
comprising at least a portion of a FGF and at least a portion of at
least one other polypeptide to form an enhanced targeting domain
with at least one property different from the reference FGF (see
Tables 7), with the proviso that this FGF chimeric can specifically
bind to an endogenous Clostridial toxin receptor present in a
Clostridial toxin target cell, and thus participate in executing
the overall cellular mechanism whereby a Clostridial toxin
proteolytically cleaves a substrate.
[0613] Thus, in an embodiment, a modified Clostridial toxin
disclosed in the present specification comprises an enhanced
targeting domain comprising a .beta.-trefoil domain derived from a
FGF. In an aspect of this embodiment, a .beta.-trefoil domain
derived from a FGF comprises, e.g., a .beta.-trefoil domain derived
from a FGF-1, a .beta.-trefoil domain derived from a FGF-2, a
.beta.-trefoil domain derived from FGF-4, a .beta.-trefoil domain
derived from a FGF-8, a .beta.-trefoil domain derived from a FGF-9,
a .beta.-trefoil domain derived from a FGF-17 or a .beta.-trefoil
domain derived from a FGF-18. In another aspect of this embodiment,
a .beta.-trefoil domain derived from a FGF comprises a .alpha.-fold
motif of a .beta.-trefoil domain of a FGF, a .beta.-fold motif of a
.beta.-trefoil domain of a FGF or a .gamma.-fold motif of a
.beta.-trefoil domain of a FGF.
[0614] In an embodiment, a modified Clostridial toxin disclosed in
the present specification comprises an enhanced targeting domain
comprising a .beta.-trefoil domain with enhanced binding activity
derived from a FGF. In an aspect of this embodiment, a
.beta.-trefoil domain with enhanced binding activity derived from a
FGF comprises, e.g., a .beta.-trefoil domain with enhanced binding
activity derived from a FGF-1, a .beta.-trefoil domain with
enhanced binding activity derived from a FGF-2, a .beta.-trefoil
domain with enhanced binding activity derived from a FGF-4, a
.beta.-trefoil domain with enhanced binding activity derived from a
FGF-8, a .beta.-trefoil domain with enhanced binding activity
derived from a FGF-9, a .beta.-trefoil domain with enhanced binding
activity derived from a FGF-17 or a .beta.-trefoil domain with
enhanced binding activity derived from a FGF-18. In another aspect
of this embodiment, a .beta.-trefoil domain with enhanced binding
activity derived from a FGF comprises a modified .alpha.-fold motif
of a .beta.-trefoil domain of a FGF, a modified .beta.-fold motif
of a .beta.-trefoil domain of a FGF or a modified .gamma.-fold
motif of a .beta.-trefoil domain of a FGF.
[0615] In another embodiment, a .beta.-trefoil domain derived from
a FGF-1 comprises a .beta.-trefoil domain derived from a FGF-1 of
SEQ ID NO: 33. In another embodiment, a .beta.-trefoil domain
derived from a FGF-1 comprises amino acids 26-155 of SEQ ID NO: 33.
In another aspect of this embodiment, a .beta.-trefoil domain
derived from a FGF-1 comprises a .alpha.-fold motif of a
.beta.-trefoil domain of a FGF-1, a .beta.-fold motif of a
.beta.-trefoil domain of a FGF-1 or a .gamma.-fold motif of a
.beta.-trefoil domain of a FGF-1 of SEQ ID NO: 33. In another
aspect of this embodiment, a .beta.-trefoil domain derived from a
FGF-1 comprises amino acids 26-64, amino acids 68-105, or amino
acids 109-155 of SEQ ID NO: 33.
[0616] In another embodiment, a .beta.-trefoil domain with enhanced
binding activity derived from a FGF-1 comprises a .beta.-trefoil
domain with enhanced binding activity derived from a FGF-1 of SEQ
ID NO: 33. In another embodiment, a .beta.-trefoil domain with
enhanced binding activity derived from a FGF-1 comprises amino
acids 26-155 of SEQ ID NO: 33. In another aspect of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a FGF-1 comprises a modified .alpha.-fold motif of a
.beta.-trefoil domain of a FGF-1, a modified .beta.-fold motif of a
.beta.-trefoil domain of a FGF-1 or a modified .gamma.-fold motif
of a .beta.-trefoil domain of a FGF-1 of SEQ ID NO: 33. In another
aspect of this embodiment, a .beta.-trefoil domain with enhanced
binding activity derived from a FGF-1 comprises amino acids 26-64,
amino acids 68-105, or amino acids 109-155 of SEQ ID NO: 33.
[0617] In other aspects of this embodiment, a FGF-1 comprising a
.beta.-trefoil domain with enhanced binding activity comprises a
polypeptide having, e.g., at least 70% amino acid identity with
amino acids 26-64, amino acids 68-105, or amino acids 109-155 of
SEQ ID NO: 33, at least 75% amino acid identity with amino acids
26-64, amino acids 68-105, or amino acids 109-155 of SEQ ID NO: 33,
at least 80% amino acid identity with amino acids 26-64, amino
acids 68-105, or amino acids 109-155 of SEQ ID NO: 33, at least 85%
amino acid identity with amino acids 26-64, amino acids 68-105, or
amino acids 109-155 of SEQ ID NO: 33, at least 90% amino acid
identity with amino acids 26-64, amino acids 68-105, or amino acids
109-155 of SEQ ID NO: 33 or at least 95% amino acid identity with
amino acids 26-64, amino acids 68-105, or amino acids 109-155 of
SEQ ID NO: 33. In yet other aspects of this embodiment, a FGF-1
comprising a .beta.-trefoil domain with enhanced binding activity
comprises a polypeptide having, e.g., at most 70% amino acid
identity with amino acids 26-64, amino acids 68-105, or amino acids
109-155 of SEQ ID NO: 33, at most 75% amino acid identity with
amino acids 26-64, amino acids 68-105, or amino acids 109-155 of
SEQ ID NO: 33, at most 80% amino acid identity with amino acids
26-64, amino acids 68-105, or amino acids 109-155 of SEQ ID NO: 33,
at most 85% amino acid identity with amino acids 26-64, amino acids
68-105, or amino acids 109-155 of SEQ ID NO: 33, at most 90% amino
acid identity with amino acids 26-64, amino acids 68-105, or amino
acids 109-155 of SEQ ID NO: 33 or at most 95% amino acid identity
with amino acids 26-64, amino acids 68-105, or amino acids 109-155
of SEQ ID NO: 33.
[0618] In other aspects of this embodiment, a FGF-1 comprising a
.beta.-trefoil domain with enhanced binding activity comprises a
polypeptide having, e.g., at most one, two, three, four, five, six,
seven, eight, nine, 10 or 20 non-contiguous amino acid
substitutions relative to amino acids 26-64, amino acids 68-105, or
amino acids 109-155 of SEQ ID NO: 33. In other aspects of this
embodiment, a FGF-1 comprising a .beta.-trefoil domain with
enhanced binding activity comprises a polypeptide having, e.g., at
least one, two, three, four, five, six, seven, eight, nine, 10 or
20 non-contiguous amino acid substitutions relative to amino acids
26-64, amino acids 68-105, or amino acids 109-155 of SEQ ID NO: 33.
In yet other aspects of this embodiment, a FGF-1 comprising a
.beta.-trefoil domain with enhanced binding activity comprises a
polypeptide having, e.g., at most one, two, three, four, five, six,
seven, eight, nine, 10 or 20 non-contiguous amino acid deletions
relative to amino acids 26-64, amino acids 68-105, or amino acids
109-155 of SEQ ID NO: 33. In other aspects of this embodiment, a
FGF-1 comprising a .beta.-trefoil domain with enhanced binding
activity comprises a polypeptide having, e.g., at least one, two,
three, four, five, six, seven, eight, nine, 10 or 20 non-contiguous
amino acid deletions relative to amino acids 26-64, amino acids
68-105, or amino acids 109-155 of SEQ ID NO: 33. In still other
aspects of this embodiment, a FGF-1 comprising a .beta.-trefoil
domain with enhanced binding activity comprises a polypeptide
having, e.g., at most one, two, three, four, five, six, seven,
eight, nine, 10 or 20 non-contiguous amino acid additions relative
to amino acids 26-64, amino acids 68-105, or amino acids 109-155 of
SEQ ID NO: 33. In other aspects of this embodiment, a FGF-1
comprising a .beta.-trefoil domain with enhanced binding activity
comprises a polypeptide having, e.g., at least one, two, three,
four, five, six, seven, eight, nine, 10 or 20 non-contiguous amino
acid additions relative to amino acids 26-64, amino acids 68-105,
or amino acids 109-155 of SEQ ID NO: 33.
[0619] In other aspects of this embodiment, a FGF-1 comprising a
.beta.-trefoil domain with enhanced binding activity comprises a
polypeptide having, e.g., at most one, two, three, four, five, six,
seven, eight, nine, 10 or 20 contiguous amino acid substitutions
relative to amino acids 26-64, amino acids 68-105, or amino acids
109-155 of SEQ ID NO: 33. In other aspects of this embodiment, a
FGF-1 comprising a .beta.-trefoil domain with enhanced binding
activity comprises a polypeptide having, e.g., at least one, two,
three, four, five, six, seven, eight, nine, 10 or 20 contiguous
amino acid substitutions relative to amino acids 26-64, amino acids
68-105, or amino acids 109-155 of SEQ ID NO: 33. In yet other
aspects of this embodiment, a FGF-1 comprising a .beta.-trefoil
domain with enhanced binding activity comprises a polypeptide
having, e.g., at most one, two, three, four, five, six, seven,
eight, nine, 10 or 20 contiguous amino acid deletions relative to
amino acids 26-64, amino acids 68-105, or amino acids 109-155 of
SEQ ID NO: 33. In other aspects of this embodiment, a FGF-1
comprising a .beta.-trefoil domain with enhanced binding activity
comprises a polypeptide having, e.g., at least one, two, three,
four, five, six, seven, eight, nine, 10 or 20 contiguous amino acid
deletions relative to amino acids 26-64, amino acids 68-105, or
amino acids 109-155 of SEQ ID NO: 33. In still other aspects of
this embodiment, a FGF-1 comprising a .beta.-trefoil domain with
enhanced binding activity comprises a polypeptide having, e.g., at
most one, two, three, four, five, six, seven, eight, nine, 10 or 20
contiguous amino acid additions relative to amino acids 26-64,
amino acids 68-105, or amino acids 109-155 of SEQ ID NO: 33. In
other aspects of this embodiment, a FGF-1 comprising a
.beta.-trefoil domain with enhanced binding activity comprises a
polypeptide having, e.g., at least one, two, three, four, five,
six, seven, eight, nine, 10 or 20 contiguous amino acid additions
relative to amino acids 26-64, amino acids 68-105, or amino acids
109-155 of SEQ ID NO: 33.
[0620] In another embodiment, a .beta.-trefoil domain derived from
a FGF-1 comprises a .beta.4/.beta.5 hairpin turn of a
.beta.-trefoil domain of a FGF-1 or a .beta.8/.beta.9 hairpin turn
of a .beta.-trefoil domain of a FGF-1 of SEQ ID NO: 33. In another
aspect of this embodiment, a .beta.-trefoil domain derived from a
FGF-1 comprises amino acids 65-67 or amino acids 106-108 of SEQ ID
NO: 33.
[0621] In another embodiment, a .beta.-trefoil domain with enhanced
binding activity derived from a FGF-1 comprises a modified
.beta.4/.beta.5 hairpin turn of a .beta.-trefoil domain of a FGF-1
or a modified .beta.8/.beta.9 hairpin turn of a .beta.-trefoil
domain of a FGF-1 of SEQ ID NO: 33. In another aspect of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a FGF-1 comprises a modification of amino acids 65-67
or amino acids 106-108 of SEQ ID NO: 33.
[0622] In other aspects of this embodiment, a .beta.-trefoil domain
with enhanced binding activity derived from a FGF-1 comprises a
polypeptide having, e.g., at least 70% amino acid identity with
amino acids 65-67 or amino acids 106-108 of SEQ ID NO: 33, at least
75% amino acid identity with amino acids 65-67 or amino acids
106-108 of SEQ ID NO: 33, at least 80% amino acid identity with
amino acids 65-67 or amino acids 106-108 of SEQ ID NO: 33, at least
85% amino acid identity with amino acids 65-67 or amino acids
106-108 of SEQ ID NO: 33, at least 90% amino acid identity with
amino acids 65-67 or amino acids 106-108 of SEQ ID NO: 33 or at
least 95% amino acid identity with amino acids 65-67 or amino acids
106-108 of SEQ ID NO: 33. In yet other aspects of this embodiment,
a .beta.-trefoil domain with enhanced binding activity derived from
a FGF-1 comprises a polypeptide having, e.g., at most 70% amino
acid identity with amino acids 65-67 or amino acids 106-108 of SEQ
ID NO: 33, at most 75% amino acid identity with amino acids 65-67
or amino acids 106-108 of SEQ ID NO: 33, at most 80% amino acid
identity with amino acids 65-67 or amino acids 106-108 of SEQ ID
NO: 33, at most 85% amino acid identity with amino acids 65-67 or
amino acids 106-108 of SEQ ID NO: 33, at most 90% amino acid
identity with amino acids 65-67 or amino acids 106-108 of SEQ ID
NO: 33 or at most 95% amino acid identity with amino acids 65-67 or
amino acids 106-108 of SEQ ID NO: 33.
[0623] In other aspects of this embodiment, a .beta.-trefoil domain
with enhanced binding activity derived from a FGF-1 comprises a
polypeptide having, e.g., at most one, two, three or four
non-contiguous amino acid substitutions relative to amino acids
65-67 or amino acids 106-108 of SEQ ID NO: 33. In other aspects of
this embodiment, a .beta.-trefoil domain with enhanced binding
activity derived from a FGF-1 comprises a polypeptide having, e.g.,
at least one, two, three or four non-contiguous amino acid
substitutions relative to amino acids 65-67 or amino acids 106-108
of SEQ ID NO: 33. In other aspects of this embodiment, a
non-contiguous amino acid substitution of any amino acid from amino
acids 65-67 or amino acids 106-108 of SEQ ID NO: 33 can be replaced
with glycine. In other aspects of this embodiment, a non-contiguous
amino acid substitution of any hydrophobic amino acid from amino
acids 65-67 or amino acids 106-108 of SEQ ID NO: 33 can be replaced
with phenylalanine. In yet other aspects of this embodiment, a
.beta.-trefoil domain with enhanced binding activity derived from a
FGF-1 comprises a polypeptide having, e.g., at most one, two, three
or four non-contiguous amino acid deletions relative to amino acids
65-67 or amino acids 106-108 of SEQ ID NO: 33. In other aspects of
this embodiment, a .beta.-trefoil domain with enhanced binding
activity derived from a FGF-1 comprises a polypeptide having, e.g.,
at least one, two, three or four non-contiguous amino acid
deletions relative to amino acids 65-67 or amino acids 106-108 of
SEQ ID NO: 33. In still other aspects of this embodiment, a
.beta.-trefoil domain with enhanced binding activity derived from a
FGF-1 comprises a polypeptide having, e.g., at most one, two, three
or four non-contiguous amino acid additions relative to amino acids
65-67 or amino acids 106-108 of SEQ ID NO: 33. In other aspects of
this embodiment, a .beta.-trefoil domain with enhanced binding
activity derived from a FGF-1 comprises a polypeptide having, e.g.,
at least one, two, three or four non-contiguous amino acid
additions relative to amino acids 65-67 or amino acids 106-108 of
SEQ ID NO: 33.
[0624] In other aspects of this embodiment, a .beta.-trefoil domain
with enhanced binding activity derived from a FGF-1 comprises a
polypeptide having, e.g., at most one, two, three or four
contiguous amino acid substitutions relative to amino acids 65-67
or amino acids 106-108 of SEQ ID NO: 33. In other aspects of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a FGF-1 comprises a polypeptide having, e.g., at least
one, two, three or four contiguous amino acid substitutions
relative to amino acids 65-67 or amino acids 106-108 of SEQ ID NO:
33. In other aspects of this embodiment, contiguous amino acid
substitutions of amino acids from amino acids 65-67 or amino acids
106-108 of SEQ ID NO: 33 can be replaced with glycine. In other
aspects of this embodiment, contiguous amino acid substitutions of
hydrophobic amino acids from amino acids 65-67 or amino acids
106-108 of SEQ ID NO: 33 can be replaced with phenylalanine. In yet
other aspects of this embodiment, a .beta.-trefoil domain with
enhanced binding activity derived from a FGF-1 comprises a
polypeptide having, e.g., at most one, two, three or four
contiguous amino acid deletions relative to amino acids 65-67 or
amino acids 106-108 of SEQ ID NO: 33. In other aspects of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a FGF-1 comprises a polypeptide having, e.g., at least
one, two, three or four contiguous amino acid deletions relative to
amino acids 65-67 or amino acids 106-108 of SEQ ID NO: 33. In still
other aspects of this embodiment, a .beta.-trefoil domain with
enhanced binding activity derived from a FGF-1 comprises a
polypeptide having, e.g., at most one, two, three or four
contiguous amino acid additions relative to amino acids 65-67 or
amino acids 106-108 of SEQ ID NO: 33. In other aspects of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a FGF-1 comprises a polypeptide having, e.g., at least
one, two, three or four contiguous amino acid additions relative to
amino acids 65-67 or amino acids 106-108 of SEQ ID NO: 33.
[0625] In another embodiment, a .beta.-trefoil domain derived from
a FGF-2 comprises a .beta.-trefoil domain derived from a FGF-2 of
SEQ ID NO: 34. In another embodiment, a .beta.-trefoil domain
derived from a FGF-2 comprises amino acids 29-155 of SEQ ID NO: 34.
In another aspect of this embodiment, a .beta.-trefoil domain
derived from a FGF-2 comprises a .alpha.-fold motif of a
.beta.-trefoil domain of a FGF-2, a .beta.-fold motif of a
.beta.-trefoil domain of a FGF-2 or a .gamma.-fold motif of a
.beta.-trefoil domain of a FGF-2 of SEQ ID NO: 34. In another
aspect of this embodiment, a .beta.-trefoil domain derived from a
FGF-2 comprises amino acids 29-67, amino acids 71-111, or amino
acids 115-155 of SEQ ID NO: 34.
[0626] In another embodiment, a .beta.-trefoil domain with enhanced
binding activity derived from a FGF-2 comprises a .beta.-trefoil
domain with enhanced binding activity derived from a FGF-2 of SEQ
ID NO: 34. In another embodiment, a .beta.-trefoil domain with
enhanced binding activity derived from a FGF-2 comprises amino
acids 29-155 of SEQ ID NO: 34. In another aspect of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a FGF-2 comprises a modified .alpha.-fold motif of a
.beta.-trefoil domain of a FGF-2, a modified .beta.-fold motif of a
.beta.-trefoil domain of a FGF-2 or a modified .gamma.-fold motif
of a .beta.-trefoil domain of a FGF-2 of SEQ ID NO: 34. In another
aspect of this embodiment, a .beta.-trefoil domain with enhanced
binding activity derived from a FGF-2 comprises amino acids 29-67,
amino acids 71-111, or amino acids 115-155 of SEQ ID NO: 34.
[0627] In other aspects of this embodiment, a FGF-2 comprising a
.beta.-trefoil domain with enhanced binding activity comprises a
polypeptide having, e.g., at least 70% amino acid identity with
amino acids 29-67, amino acids 71-111, or amino acids 115-155 of
SEQ ID NO: 34, at least 75% amino acid identity with amino acids
29-67, amino acids 71-111, or amino acids 115-155 of SEQ ID NO: 34,
at least 80% amino acid identity with amino acids 29-67, amino
acids 71-111, or amino acids 115-155 of SEQ ID NO: 34, at least 85%
amino acid identity with amino acids 29-67, amino acids 71-111, or
amino acids 115-155 of SEQ ID NO: 34, at least 90% amino acid
identity with amino acids 29-67, amino acids 71-111, or amino acids
115-155 of SEQ ID NO: 34 or at least 95% amino acid identity with
amino acids 29-67, amino acids 71-111, or amino acids 115-155 of
SEQ ID NO: 34. In yet other aspects of this embodiment, a FGF-2
comprising a .beta.-trefoil domain with enhanced binding activity
comprises a polypeptide having, e.g., at most 70% amino acid
identity with amino acids 29-67, amino acids 71-111, or amino acids
115-155 of SEQ ID NO: 34, at most 75% amino acid identity with
amino acids 29-67, amino acids 71-111, or amino acids 115-155 of
SEQ ID NO: 34, at most 80% amino acid identity with amino acids
29-67, amino acids 71-111, or amino acids 115-155 of SEQ ID NO: 34,
at most 85% amino acid identity with amino acids 29-67, amino acids
71-111, or amino acids 115-155 of SEQ ID NO: 34, at most 90% amino
acid identity with amino acids 29-67, amino acids 71-111, or amino
acids 115-155 of SEQ ID NO: 34 or at most 95% amino acid identity
with amino acids 29-67, amino acids 71-111, or amino acids 115-155
of SEQ ID NO: 34.
[0628] In other aspects of this embodiment, a FGF-2 comprising a
.beta.-trefoil domain with enhanced binding activity comprises a
polypeptide having, e.g., at most one, two, three, four, five, six,
seven, eight, nine, 10 or 20 non-contiguous amino acid
substitutions relative to amino acids 29-67, amino acids 71-111, or
amino acids 115-155 of SEQ ID NO: 34. In other aspects of this
embodiment, a FGF-2 comprising a .beta.-trefoil domain with
enhanced binding activity comprises a polypeptide having, e.g., at
least one, two, three, four, five, six, seven, eight, nine, 10 or
20 non-contiguous amino acid substitutions relative to amino acids
29-67, amino acids 71-111, or amino acids 115-155 of SEQ ID NO: 34.
In yet other aspects of this embodiment, a FGF-2 comprising a
.beta.-trefoil domain with enhanced binding activity comprises a
polypeptide having, e.g., at most one, two, three, four, five, six,
seven, eight, nine, 10 or 20 non-contiguous amino acid deletions
relative to amino acids 29-67, amino acids 71-111, or amino acids
115-155 of SEQ ID NO: 34. In other aspects of this embodiment, a
FGF-2 comprising a .beta.-trefoil domain with enhanced binding
activity comprises a polypeptide having, e.g., at least one, two,
three, four, five, six, seven, eight, nine, 10 or 20 non-contiguous
amino acid deletions relative to amino acids 29-67, amino acids
71-111, or amino acids 115-155 of SEQ ID NO: 34. In still other
aspects of this embodiment, a FGF-2 comprising a .beta.-trefoil
domain with enhanced binding activity comprises a polypeptide
having, e.g., at most one, two, three, four, five, six, seven,
eight, nine, 10 or 20 non-contiguous amino acid additions relative
to amino acids 29-67, amino acids 71-111, or amino acids 115-155 of
SEQ ID NO: 34. In other aspects of this embodiment, a FGF-2
comprising a .beta.-trefoil domain with enhanced binding activity
comprises a polypeptide having, e.g., at least one, two, three,
four, five, six, seven, eight, nine, 10 or 20 non-contiguous amino
acid additions relative to amino acids 29-67, amino acids 71-111,
or amino acids 115-155 of SEQ ID NO: 34.
[0629] In other aspects of this embodiment, a FGF-2 comprising a
.beta.-trefoil domain with enhanced binding activity comprises a
polypeptide having, e.g., at most one, two, three, four, five, six,
seven, eight, nine, 10 or 20 contiguous amino acid substitutions
relative to amino acids 29-67, amino acids 71-111, or amino acids
115-155 of SEQ ID NO: 34. In other aspects of this embodiment, a
FGF-2 comprising a .beta.-trefoil domain with enhanced binding
activity comprises a polypeptide having, e.g., at least one, two,
three, four, five, six, seven, eight, nine, 10 or 20 contiguous
amino acid substitutions relative to amino acids 29-67, amino acids
71-111, or amino acids 115-155 of SEQ ID NO: 34. In yet other
aspects of this embodiment, a FGF-2 comprising a .beta.-trefoil
domain with enhanced binding activity comprises a polypeptide
having, e.g., at most one, two, three, four, five, six, seven,
eight, nine, 10 or 20 contiguous amino acid deletions relative to
amino acids 29-67, amino acids 71-111, or amino acids 115-155 of
SEQ ID NO: 34. In other aspects of this embodiment, a FGF-2
comprising a .beta.-trefoil domain with enhanced binding activity
comprises a polypeptide having, e.g., at least one, two, three,
four, five, six, seven, eight, nine, 10 or 20 contiguous amino acid
deletions relative to amino acids 29-67, amino acids 71-111, or
amino acids 115-155 of SEQ ID NO: 34. In still other aspects of
this embodiment, a FGF-2 comprising a .beta.-trefoil domain with
enhanced binding activity comprises a polypeptide having, e.g., at
most one, two, three, four, five, six, seven, eight, nine, 10 or 20
contiguous amino acid additions relative to amino acids 29-67,
amino acids 71-111, or amino acids 115-155 of SEQ ID NO: 34. In
other aspects of this embodiment, a FGF-2 comprising a
.beta.-trefoil domain with enhanced binding activity comprises a
polypeptide having, e.g., at least one, two, three, four, five,
six, seven, eight, nine, 10 or 20 contiguous amino acid additions
relative to amino acids 29-67, amino acids 71-111, or amino acids
115-155 of SEQ ID NO: 34.
[0630] In another embodiment, a .beta.-trefoil domain derived from
a FGF-2 comprises a .beta.4/.beta.5 hairpin turn of a
.beta.-trefoil domain of a FGF-2 or a .beta.8/.beta.9 hairpin turn
of a .beta.-trefoil domain of a FGF-2 of SEQ ID NO: 34. In another
aspect of this embodiment, a .beta.-trefoil domain derived from a
FGF-2 comprises amino acids 68-70 or amino acids 112-114 of SEQ ID
NO: 34.
[0631] In another embodiment, a .beta.-trefoil domain with enhanced
binding activity derived from a FGF-2 comprises a modified
.beta.4/.beta.5 hairpin turn of a .beta.-trefoil domain of a FGF-2
or a modified .beta.8/.beta.9 hairpin turn of a .beta.-trefoil
domain of a FGF-2 of SEQ ID NO: 34. In another aspect of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a FGF-2 comprises a modification of amino acids 68-70
or amino acids 112-114 of SEQ ID NO: 34.
[0632] In other aspects of this embodiment, a .beta.-trefoil domain
with enhanced binding activity derived from a FGF-2 comprises a
polypeptide having, e.g., at least 70% amino acid identity with
amino acids 68-70 or amino acids 112-114 of SEQ ID NO: 34, at least
75% amino acid identity with amino acids 68-70 or amino acids
112-114 of SEQ ID NO: 34, at least 80% amino acid identity with
amino acids 68-70 or amino acids 112-114 of SEQ ID NO: 34, at least
85% amino acid identity with amino acids 68-70 or amino acids
112-114 of SEQ ID NO: 34, at least 90% amino acid identity with
amino acids 68-70 or amino acids 112-114 of SEQ ID NO: 34 or at
least 95% amino acid identity with amino acids 68-70 or amino acids
112-114 of SEQ ID NO: 34. In yet other aspects of this embodiment,
a .beta.-trefoil domain with enhanced binding activity derived from
a FGF-2 comprises a polypeptide having, e.g., at most 70% amino
acid identity with amino acids 68-70 or amino acids 112-114 of SEQ
ID NO: 34, at most 75% amino acid identity with amino acids 68-70
or amino acids 112-114 of SEQ ID NO: 34, at most 80% amino acid
identity with amino acids 68-70 or amino acids 112-114 of SEQ ID
NO: 34, at most 85% amino acid identity with amino acids 68-70 or
amino acids 112-114 of SEQ ID NO: 34, at most 90% amino acid
identity with amino acids 68-70 or amino acids 112-114 of SEQ ID
NO: 34 or at most 95% amino acid identity with amino acids 68-70 or
amino acids 112-114 of SEQ ID NO: 34.
[0633] In other aspects of this embodiment, a .beta.-trefoil domain
with enhanced binding activity derived from a FGF-2 comprises a
polypeptide having, e.g., at most one, two, three or four
non-contiguous amino acid substitutions relative to amino acids
68-70 or amino acids 112-114 of SEQ ID NO: 34. In other aspects of
this embodiment, a .beta.-trefoil domain with enhanced binding
activity derived from a FGF-2 comprises a polypeptide having, e.g.,
at least one, two, three or four non-contiguous amino acid
substitutions relative to amino acids 68-70 or amino acids 112-114
of SEQ ID NO: 34. In other aspects of this embodiment, a
non-contiguous amino acid substitution of any amino acid from amino
acids 68-70 or amino acids 112-114 of SEQ ID NO: 34 can be replaced
with glycine. In other aspects of this embodiment, a non-contiguous
amino acid substitution of any hydrophobic amino acid from amino
acids 68-70 or amino acids 112-114 of SEQ ID NO: 34 can be replaced
with phenylalanine. In yet other aspects of this embodiment, a
.beta.-trefoil domain with enhanced binding activity derived from a
FGF-2 comprises a polypeptide having, e.g., at most one, two, three
or four non-contiguous amino acid deletions relative to amino acids
68-70 or amino acids 112-114 of SEQ ID NO: 34. In other aspects of
this embodiment, a .beta.-trefoil domain with enhanced binding
activity derived from a FGF-2 comprises a polypeptide having, e.g.,
at least one, two, three or four non-contiguous amino acid
deletions relative to amino acids 68-70 or amino acids 112-114 of
SEQ ID NO: 34. In still other aspects of this embodiment, a
.beta.-trefoil domain with enhanced binding activity derived from a
FGF-2 comprises a polypeptide having, e.g., at most one, two, three
or four non-contiguous amino acid additions relative to amino acids
68-70 or amino acids 112-114 of SEQ ID NO: 34. In other aspects of
this embodiment, a .beta.-trefoil domain with enhanced binding
activity derived from a FGF-2 comprises a polypeptide having, e.g.,
at least one, two, three or four non-contiguous amino acid
additions relative to amino acids 68-70 or amino acids 112-114 of
SEQ ID NO: 34.
[0634] In other aspects of this embodiment, a .beta.-trefoil domain
with enhanced binding activity derived from a FGF-2 comprises a
polypeptide having, e.g., at most one, two, three or four
contiguous amino acid substitutions relative to amino acids 68-70
or amino acids 112-114 of SEQ ID NO: 34. In other aspects of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a FGF-2 comprises a polypeptide having, e.g., at least
one, two, three or four contiguous amino acid substitutions
relative to amino acids 68-70 or amino acids 112-114 of SEQ ID NO:
34. In other aspects of this embodiment, contiguous amino acid
substitutions of amino acids from amino acids 68-70 or amino acids
112-114 of SEQ ID NO: 34 can be replaced with glycine. In other
aspects of this embodiment, contiguous amino acid substitutions of
hydrophobic amino acids from amino acids 68-70 or amino acids
112-114 of SEQ ID NO: 34 can be replaced with phenylalanine. In yet
other aspects of this embodiment, a .beta.-trefoil domain with
enhanced binding activity derived from a FGF-2 comprises a
polypeptide having, e.g., at most one, two, three or four
contiguous amino acid deletions relative to amino acids 68-70 or
amino acids 112-114 of SEQ ID NO: 34. In other aspects of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a FGF-2 comprises a polypeptide having, e.g., at least
one, two, three or four contiguous amino acid deletions relative to
amino acids 68-70 or amino acids 112-114 of SEQ ID NO: 34. In still
other aspects of this embodiment, a .beta.-trefoil domain with
enhanced binding activity derived from a FGF-2 comprises a
polypeptide having, e.g., at most one, two, three or four
contiguous amino acid additions relative to amino acids 68-70 or
amino acids 112-114 of SEQ ID NO: 34. In other aspects of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a FGF-2 comprises a polypeptide having, e.g., at least
one, two, three or four contiguous amino acid additions relative to
amino acids 68-70 or amino acids 112-114 of SEQ ID NO: 34.
[0635] In another embodiment, a .beta.-trefoil domain derived from
a FGF-4 comprises a .beta.-trefoil domain derived from a FGF-4 of
SEQ ID NO: 35. In another embodiment, a .beta.-trefoil domain
derived from a FGF-4 comprises amino acids 83-206 of SEQ ID NO: 35.
In another aspect of this embodiment, a .beta.-trefoil domain
derived from a FGF-4 comprises a .alpha.-fold motif of a
.beta.-trefoil domain of a FGF-4, a .beta.-fold motif of a
.beta.-trefoil domain of a FGF-4 or a .gamma.-fold motif of a
.beta.-trefoil domain of a FGF-4 of SEQ ID NO: 35. In another
aspect of this embodiment, a .beta.-trefoil domain derived from a
FGF-4 comprises amino acids 83-121, amino acids 125-162, or amino
acids 166-206 of SEQ ID NO: 35.
[0636] In another embodiment, a .beta.-trefoil domain with enhanced
binding activity derived from a FGF-4 comprises a .beta.-trefoil
domain with enhanced binding activity derived from a FGF-4 of SEQ
ID NO: 35. In another embodiment, a .beta.-trefoil domain with
enhanced binding activity derived from a FGF-4 comprises amino
acids 83-206 of SEQ ID NO: 35. In another aspect of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a FGF-4 comprises a modified .alpha.-fold motif of a
.beta.-trefoil domain of a FGF-4, a modified .beta.-fold motif of a
.beta.-trefoil domain of a FGF-4 or a modified .gamma.-fold motif
of a .beta.-trefoil domain of a FGF-4 of SEQ ID NO: 35. In another
aspect of this embodiment, a .beta.-trefoil domain with enhanced
binding activity derived from a FGF-4 comprises amino acids 83-121,
amino acids 125-162, or amino acids 166-206 of SEQ ID NO: 35.
[0637] In other aspects of this embodiment, a FGF-4 comprising a
.beta.-trefoil domain with enhanced binding activity comprises a
polypeptide having, e.g., at least 70% amino acid identity with
amino acids 83-121, amino acids 125-162, or amino acids 166-206 of
SEQ ID NO: 35, at least 75% amino acid identity with amino acids
83-121, amino acids 125-162, or amino acids 166-206 of SEQ ID NO:
35, at least 80% amino acid identity with amino acids 83-121, amino
acids 125-162, or amino acids 166-206 of SEQ ID NO: 35, at least
85% amino acid identity with amino acids 83-121, amino acids
125-162, or amino acids 166-206 of SEQ ID NO: 35, at least 90%
amino acid identity with amino acids 83-121, amino acids 125-162,
or amino acids 166-206 of SEQ ID NO: 35 or at least 95% amino acid
identity with amino acids 83-121, amino acids 125-162, or amino
acids 166-206 of SEQ ID NO: 35. In yet other aspects of this
embodiment, a FGF-4 comprising a .beta.-trefoil domain with
enhanced binding activity comprises a polypeptide having, e.g., at
most 70% amino acid identity with amino acids 83-121, amino acids
125-162, or amino acids 166-206 of SEQ ID NO: 35, at most 75% amino
acid identity with amino acids 83-121, amino acids 125-162, or
amino acids 166-206 of SEQ ID NO: 35, at most 80% amino acid
identity with amino acids 83-121, amino acids 125-162, or amino
acids 166-206 of SEQ ID NO: 35, at most 85% amino acid identity
with amino acids 83-121, amino acids 125-162, or amino acids
166-206 of SEQ ID NO: 35, at most 90% amino acid identity with
amino acids 83-121, amino acids 125-162, or amino acids 166-206 of
SEQ ID NO: 35 or at most 95% amino acid identity with amino acids
83-121, amino acids 125-162, or amino acids 166-206 of SEQ ID NO:
35.
[0638] In other aspects of this embodiment, a FGF-4 comprising a
.beta.-trefoil domain with enhanced binding activity comprises a
polypeptide having, e.g., at most one, two, three, four, five, six,
seven, eight, nine, 10 or 20 non-contiguous amino acid
substitutions relative to amino acids 83-121, amino acids 125-162,
or amino acids 166-206 of SEQ ID NO: 35. In other aspects of this
embodiment, a FGF-4 comprising a .beta.-trefoil domain with
enhanced binding activity comprises a polypeptide having, e.g., at
least one, two, three, four, five, six, seven, eight, nine, 10 or
20 non-contiguous amino acid substitutions relative to amino acids
83-121, amino acids 125-162, or amino acids 166-206 of SEQ ID NO:
35. In yet other aspects of this embodiment, a FGF-4 comprising a
.beta.-trefoil domain with enhanced binding activity comprises a
polypeptide having, e.g., at most one, two, three, four, five, six,
seven, eight, nine, 10 or 20 non-contiguous amino acid deletions
relative to amino acids 83-121, amino acids 125-162, or amino acids
166-206 of SEQ ID NO: 35. In other aspects of this embodiment, a
FGF-4 comprising a .beta.-trefoil domain with enhanced binding
activity comprises a polypeptide having, e.g., at least one, two,
three, four, five, six, seven, eight, nine, 10 or 20 non-contiguous
amino acid deletions relative to amino acids 83-121, amino acids
125-162, or amino acids 166-206 of SEQ ID NO: 35. In still other
aspects of this embodiment, a FGF-4 comprising a .beta.-trefoil
domain with enhanced binding activity comprises a polypeptide
having, e.g., at most one, two, three, four, five, six, seven,
eight, nine, 10 or 20 non-contiguous amino acid additions relative
to amino acids 83-121, amino acids 125-162, or amino acids 166-206
of SEQ ID NO: 35. In other aspects of this embodiment, a FGF-4
comprising a .beta.-trefoil domain with enhanced binding activity
comprises a polypeptide having, e.g., at least one, two, three,
four, five, six, seven, eight, nine, 10 or 20 non-contiguous amino
acid additions relative to amino acids 83-121, amino acids 125-162,
or amino acids 166-206 of SEQ ID NO: 35.
[0639] In other aspects of this embodiment, a FGF-4 comprising a
.beta.-trefoil domain with enhanced binding activity comprises a
polypeptide having, e.g., at most one, two, three, four, five, six,
seven, eight, nine, 10 or 20 contiguous amino acid substitutions
relative to amino acids 83-121, amino acids 125-162, or amino acids
166-206 of SEQ ID NO: 35. In other aspects of this embodiment, a
FGF-4 comprising a .beta.-trefoil domain with enhanced binding
activity comprises a polypeptide having, e.g., at least one, two,
three, four, five, six, seven, eight, nine, 10 or 20 contiguous
amino acid substitutions relative to amino acids 83-121, amino
acids 125-162, or amino acids 166-206 of SEQ ID NO: 35. In yet
other aspects of this embodiment, a FGF-4 comprising a
.beta.-trefoil domain with enhanced binding activity comprises a
polypeptide having, e.g., at most one, two, three, four, five, six,
seven, eight, nine, 10 or 20 contiguous amino acid deletions
relative to amino acids 83-121, amino acids 125-162, or amino acids
166-206 of SEQ ID NO: 35. In other aspects of this embodiment, a
FGF-4 comprising a .beta.-trefoil domain with enhanced binding
activity comprises a polypeptide having, e.g., at least one, two,
three, four, five, six, seven, eight, nine, 10 or 20 contiguous
amino acid deletions relative to amino acids 83-121, amino acids
125-162, or amino acids 166-206 of SEQ ID NO: 35. In still other
aspects of this embodiment, a FGF-4 comprising a .beta.-trefoil
domain with enhanced binding activity comprises a polypeptide
having, e.g., at most one, two, three, four, five, six, seven,
eight, nine, 10 or 20 contiguous amino acid additions relative to
amino acids 83-121, amino acids 125-162, or amino acids 166-206 of
SEQ ID NO: 35. In other aspects of this embodiment, a FGF-4
comprising a .beta.-trefoil domain with enhanced binding activity
comprises a polypeptide having, e.g., at least one, two, three,
four, five, six, seven, eight, nine, 10 or 20 contiguous amino acid
additions relative to amino acids 83-121, amino acids 125-162, or
amino acids 166-206 of SEQ ID NO: 35.
[0640] In another embodiment, a .beta.-trefoil domain derived from
a FGF-4 comprises a .beta.4/.beta.5 hairpin turn of a
.beta.-trefoil domain of a FGF-4 or a .beta.8/.beta.9 hairpin turn
of a .beta.-trefoil domain of a FGF-4 of SEQ ID NO: 35. In another
aspect of this embodiment, a .beta.-trefoil domain derived from a
FGF-4 comprises amino acids 122-124 or amino acids 163-165 of SEQ
ID NO: 35.
[0641] In another embodiment, a .beta.-trefoil domain with enhanced
binding activity derived from a FGF-4 comprises a modified
.beta.4/.beta.5 hairpin turn of a .beta.-trefoil domain of a FGF-4
or a modified .beta.8/.beta.9 hairpin turn of a .beta.-trefoil
domain of a FGF-4 of SEQ ID NO: 35. In another aspect of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a FGF-4 comprises a modification of amino acids
122-124 or amino acids 163-165 of SEQ ID NO: 35.
[0642] In other aspects of this embodiment, a .beta.-trefoil domain
with enhanced binding activity derived from a FGF-4 comprises a
polypeptide having, e.g., at least 70% amino acid identity with
amino acids 122-124 or amino acids 163-165 of SEQ ID NO: 35, at
least 75% amino acid identity with amino acids 122-124 or amino
acids 163-165 of SEQ ID NO: 35, at least 80% amino acid identity
with amino acids 122-124 or amino acids 163-165 of SEQ ID NO: 35,
at least 85% amino acid identity with amino acids 122-124 or amino
acids 163-165 of SEQ ID NO: 35, at least 90% amino acid identity
with amino acids 122-124 or amino acids 163-165 of SEQ ID NO: 35 or
at least 95% amino acid identity with amino acids 122-124 or amino
acids 163-165 of SEQ ID NO: 35. In yet other aspects of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a FGF-4 comprises a polypeptide having, e.g., at most
70% amino acid identity with amino acids 122-124 or amino acids
163-165 of SEQ ID NO: 35, at most 75% amino acid identity with
amino acids 122-124 or amino acids 163-165 of SEQ ID NO: 35, at
most 80% amino acid identity with amino acids 122-124 or amino
acids 163-165 of SEQ ID NO: 35, at most 85% amino acid identity
with amino acids 122-124 or amino acids 163-165 of SEQ ID NO: 35,
at most 90% amino acid identity with amino acids 122-124 or amino
acids 163-165 of SEQ ID NO: 35 or at most 95% amino acid identity
with amino acids 122-124 or amino acids 163-165 of SEQ ID NO:
35.
[0643] In other aspects of this embodiment, a .beta.-trefoil domain
with enhanced binding activity derived from a FGF-4 comprises a
polypeptide having, e.g., at most one, two, three or four
non-contiguous amino acid substitutions relative to amino acids
122-124 or amino acids 163-165 of SEQ ID NO: 35. In other aspects
of this embodiment, a .beta.-trefoil domain with enhanced binding
activity derived from a FGF-4 comprises a polypeptide having, e.g.,
at least one, two, three or four non-contiguous amino acid
substitutions relative to amino acids 122-124 or amino acids
163-165 of SEQ ID NO: 35. In other aspects of this embodiment, a
non-contiguous amino acid substitution of any amino acid from amino
acids 122-124 or amino acids 163-165 of SEQ ID NO: 35 can be
replaced with glycine. In other aspects of this embodiment, a
non-contiguous amino acid substitution of any hydrophobic amino
acid from amino acids 122-124 or amino acids 163-165 of SEQ ID NO:
35 can be replaced with phenylalanine. In yet other aspects of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a FGF-4 comprises a polypeptide having, e.g., at most
one, two, three or four non-contiguous amino acid deletions
relative to amino acids 122-124 or amino acids 163-165 of SEQ ID
NO: 35. In other aspects of this embodiment, a .beta.-trefoil
domain with enhanced binding activity derived from a FGF-4
comprises a polypeptide having, e.g., at least one, two, three or
four non-contiguous amino acid deletions relative to amino acids
122-124 or amino acids 163-165 of SEQ ID NO: 35. In still other
aspects of this embodiment, a .beta.-trefoil domain with enhanced
binding activity derived from a FGF-4 comprises a polypeptide
having, e.g., at most one, two, three or four non-contiguous amino
acid additions relative to amino acids 122-124 or amino acids
163-165 of SEQ ID NO: 35. In other aspects of this embodiment, a
.beta.-trefoil domain with enhanced binding activity derived from a
FGF-4 comprises a polypeptide having, e.g., at least one, two,
three or four non-contiguous amino acid additions relative to amino
acids 122-124 or amino acids 163-165 of SEQ ID NO: 35.
[0644] In other aspects of this embodiment, a .beta.-trefoil domain
with enhanced binding activity derived from a FGF-4 comprises a
polypeptide having, e.g., at most one, two, three or four
contiguous amino acid substitutions relative to amino acids 122-124
or amino acids 163-165 of SEQ ID NO: 35. In other aspects of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a FGF-4 comprises a polypeptide having, e.g., at least
one, two, three or four contiguous amino acid substitutions
relative to amino acids 122-124 or amino acids 163-165 of SEQ ID
NO: 35. In other aspects of this embodiment, contiguous amino acid
substitutions of amino acids from amino acids 122-124 or amino
acids 163-165 of SEQ ID NO: 35 can be replaced with glycine. In
other aspects of this embodiment, contiguous amino acid
substitutions of hydrophobic amino acids from amino acids 122-124
or amino acids 163-165 of SEQ ID NO: 35 can be replaced with
phenylalanine. In yet other aspects of this embodiment, a
.beta.-trefoil domain with enhanced binding activity derived from a
FGF-4 comprises a polypeptide having, e.g., at most one, two, three
or four contiguous amino acid deletions relative to amino acids
122-124 or amino acids 163-165 of SEQ ID NO: 35. In other aspects
of this embodiment, a .beta.-trefoil domain with enhanced binding
activity derived from a FGF-4 comprises a polypeptide having, e.g.,
at least one, two, three or four contiguous amino acid deletions
relative to amino acids 122-124 or amino acids 163-165 of SEQ ID
NO: 35. In still other aspects of this embodiment, a .beta.-trefoil
domain with enhanced binding activity derived from a FGF-4
comprises a polypeptide having, e.g., at most one, two, three or
four contiguous amino acid additions relative to amino acids
122-124 or amino acids 163-165 of SEQ ID NO: 35. In other aspects
of this embodiment, a .beta.-trefoil domain with enhanced binding
activity derived from a FGF-4 comprises a polypeptide having, e.g.,
at least one, two, three or four contiguous amino acid additions
relative to amino acids 122-124 or amino acids 163-165 of SEQ ID
NO: 35.
[0645] In another embodiment, a .beta.-trefoil domain derived from
a FGF-8 comprises a .beta.-trefoil domain derived from a FGF-8 of
SEQ ID NO: 36. In another embodiment, a .beta.-trefoil domain
derived from a FGF-8 comprises amino acids 43-172 of SEQ ID NO: 36.
In another aspect of this embodiment, a .beta.-trefoil domain
derived from a FGF-8 comprises a .alpha.-fold motif of a
.beta.-trefoil domain of a FGF-8, a .beta.-fold motif of a
.beta.-trefoil domain of a FGF-8 or a .gamma.-fold motif of a
.beta.-trefoil domain of a FGF-8 of SEQ ID NO: 36. In another
aspect of this embodiment, a .beta.-trefoil domain derived from a
FGF-8 comprises amino acids 43-80, amino acids 84-123, or amino
acids 127-172 of SEQ ID NO: 36.
[0646] In another embodiment, a .beta.-trefoil domain with enhanced
binding activity derived from a FGF-8 comprises a .beta.-trefoil
domain with enhanced binding activity derived from a FGF-8 of SEQ
ID NO: 36. In another embodiment, a .beta.-trefoil domain with
enhanced binding activity derived from a FGF-8 comprises amino
acids 43-172 of SEQ ID NO: 36. In another aspect of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a FGF-8 comprises a modified .alpha.-fold motif of a
.beta.-trefoil domain of a FGF-8, a modified .beta.-fold motif of a
.beta.-trefoil domain of a FGF-8 or a modified .gamma.-fold motif
of a .beta.-trefoil domain of a FGF-8 of SEQ ID NO: 36. In another
aspect of this embodiment, a .beta.-trefoil domain with enhanced
binding activity derived from a FGF-8 comprises amino acids 43-80,
amino acids 84-123, or amino acids 127-172 of SEQ ID NO: 36.
[0647] In other aspects of this embodiment, a FGF-8 comprising a
.beta.-trefoil domain with enhanced binding activity comprises a
polypeptide having, e.g., at least 70% amino acid identity with
amino acids 43-80, amino acids 84-123, or amino acids 127-172 of
SEQ ID NO: 36, at least 75% amino acid identity with amino acids
43-80, amino acids 84-123, or amino acids 127-172 of SEQ ID NO: 36,
at least 80% amino acid identity with amino acids 43-80, amino
acids 84-123, or amino acids 127-172 of SEQ ID NO: 36, at least 85%
amino acid identity with amino acids 43-80, amino acids 84-123, or
amino acids 127-172 of SEQ ID NO: 36, at least 90% amino acid
identity with amino acids 43-80, amino acids 84-123, or amino acids
127-172 of SEQ ID NO: 36 or at least 95% amino acid identity with
amino acids 43-80, amino acids 84-123, or amino acids 127-172 of
SEQ ID NO: 36. In yet other aspects of this embodiment, a FGF-8
comprising a .beta.-trefoil domain with enhanced binding activity
comprises a polypeptide having, e.g., at most 70% amino acid
identity with amino acids 43-80, amino acids 84-123, or amino acids
127-172 of SEQ ID NO: 36, at most 75% amino acid identity with
amino acids 43-80, amino acids 84-123, or amino acids 127-172 of
SEQ ID NO: 36, at most 80% amino acid identity with amino acids
43-80, amino acids 84-123, or amino acids 127-172 of SEQ ID NO: 36,
at most 85% amino acid identity with amino acids 43-80, amino acids
84-123, or amino acids 127-172 of SEQ ID NO: 36, at most 90% amino
acid identity with amino acids 43-80, amino acids 84-123, or amino
acids 127-172 of SEQ ID NO: 36 or at most 95% amino acid identity
with amino acids 43-80, amino acids 84-123, or amino acids 127-172
of SEQ ID NO: 36.
[0648] In other aspects of this embodiment, a FGF-8 comprising a
.beta.-trefoil domain with enhanced binding activity comprises a
polypeptide having, e.g., at most one, two, three, four, five, six,
seven, eight, nine, 10 or 20 non-contiguous amino acid
substitutions relative to amino acids 43-80, amino acids 84-123, or
amino acids 127-172 of SEQ ID NO: 36. In other aspects of this
embodiment, a FGF-8 comprising a .beta.-trefoil domain with
enhanced binding activity comprises a polypeptide having, e.g., at
least one, two, three, four, five, six, seven, eight, nine, 10 or
20 non-contiguous amino acid substitutions relative to amino acids
43-80, amino acids 84-123, or amino acids 127-172 of SEQ ID NO: 36.
In yet other aspects of this embodiment, a FGF-8 comprising a
.beta.-trefoil domain with enhanced binding activity comprises a
polypeptide having, e.g., at most one, two, three, four, five, six,
seven, eight, nine, 10 or 20 non-contiguous amino acid deletions
relative to amino acids 43-80, amino acids 84-123, or amino acids
127-172 of SEQ ID NO: 36. In other aspects of this embodiment, a
FGF-8 comprising a .beta.-trefoil domain with enhanced binding
activity comprises a polypeptide having, e.g., at least one, two,
three, four, five, six, seven, eight, nine, 10 or 20 non-contiguous
amino acid deletions relative to amino acids 43-80, amino acids
84-123, or amino acids 127-172 of SEQ ID NO: 36. In still other
aspects of this embodiment, a FGF-8 comprising a .beta.-trefoil
domain with enhanced binding activity comprises a polypeptide
having, e.g., at most one, two, three, four, five, six, seven,
eight, nine, 10 or 20 non-contiguous amino acid additions relative
to amino acids 43-80, amino acids 84-123, or amino acids 127-172 of
SEQ ID NO: 36. In other aspects of this embodiment, a FGF-8
comprising a .beta.-trefoil domain with enhanced binding activity
comprises a polypeptide having, e.g., at least one, two, three,
four, five, six, seven, eight, nine, 10 or 20 non-contiguous amino
acid additions relative to amino acids 43-80, amino acids 84-123,
or amino acids 127-172 of SEQ ID NO: 36.
[0649] In other aspects of this embodiment, a FGF-8 comprising a
.beta.-trefoil domain with enhanced binding activity comprises a
polypeptide having, e.g., at most one, two, three, four, five, six,
seven, eight, nine, 10 or 20 contiguous amino acid substitutions
relative to amino acids 43-80, amino acids 84-123, or amino acids
127-172 of SEQ ID NO: 36. In other aspects of this embodiment, a
FGF-8 comprising a .beta.-trefoil domain with enhanced binding
activity comprises a polypeptide having, e.g., at least one, two,
three, four, five, six, seven, eight, nine, 10 or 20 contiguous
amino acid substitutions relative to amino acids 43-80, amino acids
84-123, or amino acids 127-172 of SEQ ID NO: 36. In yet other
aspects of this embodiment, a FGF-8 comprising a .beta.-trefoil
domain with enhanced binding activity comprises a polypeptide
having, e.g., at most one, two, three, four, five, six, seven,
eight, nine, 10 or 20 contiguous amino acid deletions relative to
amino acids 43-80, amino acids 84-123, or amino acids 127-172 of
SEQ ID NO: 36. In other aspects of this embodiment, a FGF-8
comprising a .beta.-trefoil domain with enhanced binding activity
comprises a polypeptide having, e.g., at least one, two, three,
four, five, six, seven, eight, nine, 10 or 20 contiguous amino acid
deletions relative to amino acids 43-80, amino acids 84-123, or
amino acids 127-172 of SEQ ID NO: 36. In still other aspects of
this embodiment, a FGF-8 comprising a .beta.-trefoil domain with
enhanced binding activity comprises a polypeptide having, e.g., at
most one, two, three, four, five, six, seven, eight, nine, 10 or 20
contiguous amino acid additions relative to amino acids 43-80,
amino acids 84-123, or amino acids 127-172 of SEQ ID NO: 36. In
other aspects of this embodiment, a FGF-8 comprising a
.beta.-trefoil domain with enhanced binding activity comprises a
polypeptide having, e.g., at least one, two, three, four, five,
six, seven, eight, nine, 10 or 20 contiguous amino acid additions
relative to amino acids 43-80, amino acids 84-123, or amino acids
127-172 of SEQ ID NO: 36.
[0650] In another embodiment, a .beta.-trefoil domain derived from
a FGF-8 comprises a .beta.4/.beta.5 hairpin turn of a
.beta.-trefoil domain of a FGF-8 or a .beta.8/.beta.9 hairpin turn
of a .beta.-trefoil domain of a FGF-8 of SEQ ID NO: 36. In another
aspect of this embodiment, a .beta.-trefoil domain derived from a
FGF-8 comprises amino acids 81-83 or amino acids 124-126 of SEQ ID
NO: 36.
[0651] In another embodiment, a .beta.-trefoil domain with enhanced
binding activity derived from a FGF-8 comprises a modified
.beta.4/.beta.5 hairpin turn of a .beta.-trefoil domain of a FGF-8
or a modified .beta.8/.beta.9 hairpin turn of a .beta.-trefoil
domain of a FGF-8 of SEQ ID NO: 36. In another aspect of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a FGF-8 comprises a modification of amino acids 81-83
or amino acids 124-126 of SEQ ID NO: 36.
[0652] In other aspects of this embodiment, a .beta.-trefoil domain
with enhanced binding activity derived from a FGF-8 comprises a
polypeptide having, e.g., at least 70% amino acid identity with
amino acids 81-83 or amino acids 124-126 of SEQ ID NO: 36, at least
75% amino acid identity with amino acids 81-83 or amino acids
124-126 of SEQ ID NO: 36, at least 80% amino acid identity with
amino acids 81-83 or amino acids 124-126 of SEQ ID NO: 36, at least
85% amino acid identity with amino acids 81-83 or amino acids
124-126 of SEQ ID NO: 36, at least 90% amino acid identity with
amino acids 81-83 or amino acids 124-126 of SEQ ID NO: 36 or at
least 95% amino acid identity with amino acids 81-83 or amino acids
124-126 of SEQ ID NO: 36. In yet other aspects of this embodiment,
a .beta.-trefoil domain with enhanced binding activity derived from
a FGF-8 comprises a polypeptide having, e.g., at most 70% amino
acid identity with amino acids 81-83 or amino acids 124-126 of SEQ
ID NO: 36, at most 75% amino acid identity with amino acids 81-83
or amino acids 124-126 of SEQ ID NO: 36, at most 80% amino acid
identity with amino acids 81-83 or amino acids 124-126 of SEQ ID
NO: 36, at most 85% amino acid identity with amino acids 81-83 or
amino acids 124-126 of SEQ ID NO: 36, at most 90% amino acid
identity with amino acids 81-83 or amino acids 124-126 of SEQ ID
NO: 36 or at most 95% amino acid identity with amino acids 81-83 or
amino acids 124-126 of SEQ ID NO: 36.
[0653] In other aspects of this embodiment, a .beta.-trefoil domain
with enhanced binding activity derived from a FGF-8 comprises a
polypeptide having, e.g., at most one, two, three or four
non-contiguous amino acid substitutions relative to amino acids
81-83 or amino acids 124-126 of SEQ ID NO: 36. In other aspects of
this embodiment, a .beta.-trefoil domain with enhanced binding
activity derived from a FGF-8 comprises a polypeptide having, e.g.,
at least one, two, three or four non-contiguous amino acid
substitutions relative to amino acids 81-83 or amino acids 124-126
of SEQ ID NO: 36. In other aspects of this embodiment, a
non-contiguous amino acid substitution of any amino acid from amino
acids 81-83 or amino acids 124-126 of SEQ ID NO: 36 can be replaced
with glycine. In other aspects of this embodiment, a non-contiguous
amino acid substitution of any hydrophobic amino acid from amino
acids 81-83 or amino acids 124-126 of SEQ ID NO: 36 can be replaced
with phenylalanine. In yet other aspects of this embodiment, a
.beta.-trefoil domain with enhanced binding activity derived from a
FGF-8 comprises a polypeptide having, e.g., at most one, two, three
or four non-contiguous amino acid deletions relative to amino acids
81-83 or amino acids 124-126 of SEQ ID NO: 36. In other aspects of
this embodiment, a .beta.-trefoil domain with enhanced binding
activity derived from a FGF-8 comprises a polypeptide having, e.g.,
at least one, two, three or four non-contiguous amino acid
deletions relative to amino acids 81-83 or amino acids 124-126 of
SEQ ID NO: 36. In still other aspects of this embodiment, a
.beta.-trefoil domain with enhanced binding activity derived from a
FGF-8 comprises a polypeptide having, e.g., at most one, two, three
or four non-contiguous amino acid additions relative to amino acids
81-83 or amino acids 124-126 of SEQ ID NO: 36. In other aspects of
this embodiment, a .beta.-trefoil domain with enhanced binding
activity derived from a FGF-8 comprises a polypeptide having, e.g.,
at least one, two, three or four non-contiguous amino acid
additions relative to amino acids 81-83 or amino acids 124-126 of
SEQ ID NO: 36.
[0654] In other aspects of this embodiment, a .beta.-trefoil domain
with enhanced binding activity derived from a FGF-8 comprises a
polypeptide having, e.g., at most one, two, three or four
contiguous amino acid substitutions relative to amino acids 81-83
or amino acids 124-126 of SEQ ID NO: 36. In other aspects of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a FGF-8 comprises a polypeptide having, e.g., at least
one, two, three or four contiguous amino acid substitutions
relative to amino acids 81-83 or amino acids 124-126 of SEQ ID NO:
36. In other aspects of this embodiment, contiguous amino acid
substitutions of amino acids from amino acids 81-83 or amino acids
124-126 of SEQ ID NO: 36 can be replaced with glycine. In other
aspects of this embodiment, contiguous amino acid substitutions of
hydrophobic amino acids from amino acids 81-83 or amino acids
124-126 of SEQ ID NO: 36 can be replaced with phenylalanine. In yet
other aspects of this embodiment, a .beta.-trefoil domain with
enhanced binding activity derived from a FGF-8 comprises a
polypeptide having, e.g., at most one, two, three or four
contiguous amino acid deletions relative to amino acids 81-83 or
amino acids 124-126 of SEQ ID NO: 36. In other aspects of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a FGF-8 comprises a polypeptide having, e.g., at least
one, two, three or four contiguous amino acid deletions relative to
amino acids 81-83 or amino acids 124-126 of SEQ ID NO: 36. In still
other aspects of this embodiment, a .beta.-trefoil domain with
enhanced binding activity derived from a FGF-8 comprises a
polypeptide having, e.g., at most one, two, three or four
contiguous amino acid additions relative to amino acids 81-83 or
amino acids 124-126 of SEQ ID NO: 36. In other aspects of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a FGF-8 comprises a polypeptide having, e.g., at least
one, two, three or four contiguous amino acid additions relative to
amino acids 81-83 or amino acids 124-126 of SEQ ID NO: 36.
[0655] In another embodiment, a .beta.-trefoil domain derived from
a FGF-9 comprises a .beta.-trefoil domain derived from a FGF-9 of
SEQ ID NO: 37. In another embodiment, a .beta.-trefoil domain
derived from a FGF-9 comprises amino acids 63-196 of SEQ ID NO: 37.
In another aspect of this embodiment, a .beta.-trefoil domain
derived from a FGF-9 comprises a .alpha.-fold motif of a
.beta.-trefoil domain of a FGF-9, a .beta.-fold motif of a
.beta.-trefoil domain of a FGF-9 or a .gamma.-fold motif of a
.beta.-trefoil domain of a FGF-9 of SEQ ID NO: 37. In another
aspect of this embodiment, a .beta.-trefoil domain derived from a
FGF-9 comprises amino acids 63-100, amino acids 104-144, or amino
acids 148-196 of SEQ ID NO: 37.
[0656] In another embodiment, a .beta.-trefoil domain with enhanced
binding activity derived from a FGF-9 comprises a .beta.-trefoil
domain with enhanced binding activity derived from a FGF-9 of SEQ
ID NO: 37. In another embodiment, a .beta.-trefoil domain with
enhanced binding activity derived from a FGF-9 comprises amino
acids 63-196 of SEQ ID NO: 37. In another aspect of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a FGF-9 comprises a modified .alpha.-fold motif of a
.beta.-trefoil domain of a FGF-9, a modified .beta.-fold motif of a
.beta.-trefoil domain of a FGF-9 or a modified .gamma.-fold motif
of a .beta.-trefoil domain of a FGF-9 of SEQ ID NO: 37. In another
aspect of this embodiment, a .beta.-trefoil domain with enhanced
binding activity derived from a FGF-9 comprises amino acids 63-100,
amino acids 104-144, or amino acids 148-196 of SEQ ID NO: 37.
[0657] In other aspects of this embodiment, a FGF-9 comprising a
.beta.-trefoil domain with enhanced binding activity comprises a
polypeptide having, e.g., at least 70% amino acid identity with
amino acids 63-100, amino acids 104-144, or amino acids 148-196 of
SEQ ID NO: 37, at least 75% amino acid identity with amino acids
63-100, amino acids 104-144, or amino acids 148-196 of SEQ ID NO:
37, at least 80% amino acid identity with amino acids 63-100, amino
acids 104-144, or amino acids 148-196 of SEQ ID NO: 37, at least
85% amino acid identity with amino acids 63-100, amino acids
104-144, or amino acids 148-196 of SEQ ID NO: 37, at least 90%
amino acid identity with amino acids 63-100, amino acids 104-144,
or amino acids 148-196 of SEQ ID NO: 37 or at least 95% amino acid
identity with amino acids 63-100, amino acids 104-144, or amino
acids 148-196 of SEQ ID NO: 37. In yet other aspects of this
embodiment, a FGF-9 comprising a .beta.-trefoil domain with
enhanced binding activity comprises a polypeptide having, e.g., at
most 70% amino acid identity with amino acids 63-100, amino acids
104-144, or amino acids 148-196 of SEQ ID NO: 37, at most 75% amino
acid identity with amino acids 63-100, amino acids 104-144, or
amino acids 148-196 of SEQ ID NO: 37, at most 80% amino acid
identity with amino acids 63-100, amino acids 104-144, or amino
acids 148-196 of SEQ ID NO: 37, at most 85% amino acid identity
with amino acids 63-100, amino acids 104-144, or amino acids
148-196 of SEQ ID NO: 37, at most 90% amino acid identity with
amino acids 63-100, amino acids 104-144, or amino acids 148-196 of
SEQ ID NO: 37 or at most 95% amino acid identity with amino acids
63-100, amino acids 104-144, or amino acids 148-196 of SEQ ID NO:
37.
[0658] In other aspects of this embodiment, a FGF-9 comprising a
.beta.-trefoil domain with enhanced binding activity comprises a
polypeptide having, e.g., at most one, two, three, four, five, six,
seven, eight, nine, 10 or 20 non-contiguous amino acid
substitutions relative to amino acids 63-100, amino acids 104-144,
or amino acids 148-196 of SEQ ID NO: 37. In other aspects of this
embodiment, a FGF-9 comprising a .beta.-trefoil domain with
enhanced binding activity comprises a polypeptide having, e.g., at
least one, two, three, four, five, six, seven, eight, nine, 10 or
20 non-contiguous amino acid substitutions relative to amino acids
63-100, amino acids 104-144, or amino acids 148-196 of SEQ ID NO:
37. In yet other aspects of this embodiment, a FGF-9 comprising a
.beta.-trefoil domain with enhanced binding activity comprises a
polypeptide having, e.g., at most one, two, three, four, five, six,
seven, eight, nine, 10 or 20 non-contiguous amino acid deletions
relative to amino acids 63-100, amino acids 104-144, or amino acids
148-196 of SEQ ID NO: 37. In other aspects of this embodiment, a
FGF-9 comprising a .beta.-trefoil domain with enhanced binding
activity comprises a polypeptide having, e.g., at least one, two,
three, four, five, six, seven, eight, nine, 10 or 20 non-contiguous
amino acid deletions relative to amino acids 63-100, amino acids
104-144, or amino acids 148-196 of SEQ ID NO: 37. In still other
aspects of this embodiment, a FGF-9 comprising a .beta.-trefoil
domain with enhanced binding activity comprises a polypeptide
having, e.g., at most one, two, three, four, five, six, seven,
eight, nine, 10 or 20 non-contiguous amino acid additions relative
to amino acids 63-100, amino acids 104-144, or amino acids 148-196
of SEQ ID NO: 37. In other aspects of this embodiment, a FGF-9
comprising a .beta.-trefoil domain with enhanced binding activity
comprises a polypeptide having, e.g., at least one, two, three,
four, five, six, seven, eight, nine, 10 or 20 non-contiguous amino
acid additions relative to amino acids 63-100, amino acids 104-144,
or amino acids 148-196 of SEQ ID NO: 37.
[0659] In other aspects of this embodiment, a FGF-9 comprising a
.beta.-trefoil domain with enhanced binding activity comprises a
polypeptide having, e.g., at most one, two, three, four, five, six,
seven, eight, nine, 10 or 20 contiguous amino acid substitutions
relative to amino acids 63-100, amino acids 104-144, or amino acids
148-196 of SEQ ID NO: 37. In other aspects of this embodiment, a
FGF-9 comprising a .beta.-trefoil domain with enhanced binding
activity comprises a polypeptide having, e.g., at least one, two,
three, four, five, six, seven, eight, nine, 10 or 20 contiguous
amino acid substitutions relative to amino acids 63-100, amino
acids 104-144, or amino acids 148-196 of SEQ ID NO: 37. In yet
other aspects of this embodiment, a FGF-9 comprising a
.beta.-trefoil domain with enhanced binding activity comprises a
polypeptide having, e.g., at most one, two, three, four, five, six,
seven, eight, nine, 10 or 20 contiguous amino acid deletions
relative to amino acids 63-100, amino acids 104-144, or amino acids
148-196 of SEQ ID NO: 37. In other aspects of this embodiment, a
FGF-9 comprising a .beta.-trefoil domain with enhanced binding
activity comprises a polypeptide having, e.g., at least one, two,
three, four, five, six, seven, eight, nine, 10 or 20 contiguous
amino acid deletions relative to amino acids 63-100, amino acids
104-144, or amino acids 148-196 of SEQ ID NO: 37. In still other
aspects of this embodiment, a FGF-9 comprising a .beta.-trefoil
domain with enhanced binding activity comprises a polypeptide
having, e.g., at most one, two, three, four, five, six, seven,
eight, nine, 10 or 20 contiguous amino acid additions relative to
amino acids 63-100, amino acids 104-144, or amino acids 148-196 of
SEQ ID NO: 37. In other aspects of this embodiment, a FGF-9
comprising a .beta.-trefoil domain with enhanced binding activity
comprises a polypeptide having, e.g., at least one, two, three,
four, five, six, seven, eight, nine, 10 or 20 contiguous amino acid
additions relative to amino acids 63-100, amino acids 104-144, or
amino acids 148-196 of SEQ ID NO: 37.
[0660] In another embodiment, a .beta.-trefoil domain derived from
a FGF-9 comprises a .beta.4/.beta.5 hairpin turn of a
.beta.-trefoil domain of a FGF-9 or a .beta.8/.beta.9 hairpin turn
of a .beta.-trefoil domain of a FGF-9 of SEQ ID NO: 37. In another
aspect of this embodiment, a .beta.-trefoil domain derived from a
FGF-9 comprises amino acids 101-103 or amino acids 145-147 of SEQ
ID NO: 37.
[0661] In another embodiment, a .beta.-trefoil domain with enhanced
binding activity derived from a FGF-9 comprises a modified
.beta.4/.beta.5 hairpin turn of a .beta.-trefoil domain of a FGF-9
or a modified .beta.8/.beta.9 hairpin turn of a .beta.-trefoil
domain of a FGF-9 of SEQ ID NO: 37. In another aspect of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a FGF-9 comprises a modification of amino acids
101-103 or amino acids 145-147 of SEQ ID NO: 37.
[0662] In other aspects of this embodiment, a .beta.-trefoil domain
with enhanced binding activity derived from a FGF-9 comprises a
polypeptide having, e.g., at least 70% amino acid identity with
amino acids 101-103 or amino acids 145-147 of SEQ ID NO: 37, at
least 75% amino acid identity with amino acids 101-103 or amino
acids 145-147 of SEQ ID NO: 37, at least 80% amino acid identity
with amino acids 101-103 or amino acids 145-147 of SEQ ID NO: 37,
at least 85% amino acid identity with amino acids 101-103 or amino
acids 145-147 of SEQ ID NO: 37, at least 90% amino acid identity
with amino acids 101-103 or amino acids 145-147 of SEQ ID NO: 37 or
at least 95% amino acid identity with amino acids 101-103 or amino
acids 145-147 of SEQ ID NO: 37. In yet other aspects of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a FGF-9 comprises a polypeptide having, e.g., at most
70% amino acid identity with amino acids 101-103 or amino acids
145-147 of SEQ ID NO: 37, at most 75% amino acid identity with
amino acids 101-103 or amino acids 145-147 of SEQ ID NO: 37, at
most 80% amino acid identity with amino acids 101-103 or amino
acids 145-147 of SEQ ID NO: 37, at most 85% amino acid identity
with amino acids 101-103 or amino acids 145-147 of SEQ ID NO: 37,
at most 90% amino acid identity with amino acids 101-103 or amino
acids 145-147 of SEQ ID NO: 37 or at most 95% amino acid identity
with amino acids 101-103 or amino acids 145-147 of SEQ ID NO:
37.
[0663] In other aspects of this embodiment, a .beta.-trefoil domain
with enhanced binding activity derived from a FGF-9 comprises a
polypeptide having, e.g., at most one, two, three or four
non-contiguous amino acid substitutions relative to amino acids
101-103 or amino acids 145-147 of SEQ ID NO: 37. In other aspects
of this embodiment, a .beta.-trefoil domain with enhanced binding
activity derived from a FGF-9 comprises a polypeptide having, e.g.,
at least one, two, three or four non-contiguous amino acid
substitutions relative to amino acids 101-103 or amino acids
145-147 of SEQ ID NO: 37. In other aspects of this embodiment, a
non-contiguous amino acid substitution of any amino acid from amino
acids 101-103 or amino acids 145-147 of SEQ ID NO: 37 can be
replaced with glycine. In other aspects of this embodiment, a
non-contiguous amino acid substitution of any hydrophobic amino
acid from amino acids 101-103 or amino acids 145-147 of SEQ ID NO:
37 can be replaced with phenylalanine. In yet other aspects of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a FGF-9 comprises a polypeptide having, e.g., at most
one, two, three or four non-contiguous amino acid deletions
relative to amino acids 101-103 or amino acids 145-147 of SEQ ID
NO: 37. In other aspects of this embodiment, a .beta.-trefoil
domain with enhanced binding activity derived from a FGF-9
comprises a polypeptide having, e.g., at least one, two, three or
four non-contiguous amino acid deletions relative to amino acids
101-103 or amino acids 145-147 of SEQ ID NO: 37. In still other
aspects of this embodiment, a .beta.-trefoil domain with enhanced
binding activity derived from a FGF-9 comprises a polypeptide
having, e.g., at most one, two, three or four non-contiguous amino
acid additions relative to amino acids 101-103 or amino acids
145-147 of SEQ ID NO: 37. In other aspects of this embodiment, a
.beta.-trefoil domain with enhanced binding activity derived from a
FGF-9 comprises a polypeptide having, e.g., at least one, two,
three or four non-contiguous amino acid additions relative to amino
acids 101-103 or amino acids 145-147 of SEQ ID NO: 37.
[0664] In other aspects of this embodiment, a .beta.-trefoil domain
with enhanced binding activity derived from a FGF-9 comprises a
polypeptide having, e.g., at most one, two, three or four
contiguous amino acid substitutions relative to amino acids 101-103
or amino acids 145-147 of SEQ ID NO: 37. In other aspects of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a FGF-9 comprises a polypeptide having, e.g., at least
one, two, three or four contiguous amino acid substitutions
relative to amino acids 101-103 or amino acids 145-147 of SEQ ID
NO: 37. In other aspects of this embodiment, contiguous amino acid
substitutions of amino acids from amino acids 101-103 or amino
acids 145-147 of SEQ ID NO: 37 can be replaced with glycine. In
other aspects of this embodiment, contiguous amino acid
substitutions of hydrophobic amino acids from amino acids 101-103
or amino acids 145-147 of SEQ ID NO: 37 can be replaced with
phenylalanine. In yet other aspects of this embodiment, a
.beta.-trefoil domain with enhanced binding activity derived from a
FGF-9 comprises a polypeptide having, e.g., at most one, two, three
or four contiguous amino acid deletions relative to amino acids
101-103 or amino acids 145-147 of SEQ ID NO: 37. In other aspects
of this embodiment, a .beta.-trefoil domain with enhanced binding
activity derived from a FGF-9 comprises a polypeptide having, e.g.,
at least one, two, three or four contiguous amino acid deletions
relative to amino acids 101-103 or amino acids 145-147 of SEQ ID
NO: 37. In still other aspects of this embodiment, a .beta.-trefoil
domain with enhanced binding activity derived from a FGF-9
comprises a polypeptide having, e.g., at most one, two, three or
four contiguous amino acid additions relative to amino acids
101-103 or amino acids 145-147 of SEQ ID NO: 37. In other aspects
of this embodiment, a .beta.-trefoil domain with enhanced binding
activity derived from a FGF-9 comprises a polypeptide having, e.g.,
at least one, two, three or four contiguous amino acid additions
relative to amino acids 101-103 or amino acids 145-147 of SEQ ID
NO: 37.
[0665] In another embodiment, a .beta.-trefoil domain derived from
a FGF-17 comprises a .beta.-trefoil domain derived from a FGF-17 of
SEQ ID NO: 38. In another embodiment, a .beta.-trefoil domain
derived from a FGF-17 comprises amino acids 55-183 of SEQ ID NO:
38. In another aspect of this embodiment, a .beta.-trefoil domain
derived from a FGF-17 comprises a .alpha.-fold motif of a
.beta.-trefoil domain of a FGF-17, a .beta.-fold motif of a
.beta.-trefoil domain of a FGF-17 or a .gamma.-fold motif of a
.beta.-trefoil domain of a FGF-17 of SEQ ID NO: 38. In another
aspect of this embodiment, a .beta.-trefoil domain derived from a
FGF-17 comprises amino acids 55-91, amino acids 95-134, or amino
acids 138-183 of SEQ ID NO: 38.
[0666] In another embodiment, a .beta.-trefoil domain with enhanced
binding activity derived from a FGF-17 comprises a .beta.-trefoil
domain with enhanced binding activity derived from a FGF-17 of SEQ
ID NO: 38. In another embodiment, a .beta.-trefoil domain with
enhanced binding activity derived from a FGF-17 comprises amino
acids 55-183 of SEQ ID NO: 38. In another aspect of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a FGF-17 comprises a modified .alpha.-fold motif of a
.beta.-trefoil domain of a FGF-17, a modified .beta.-fold motif of
a .beta.-trefoil domain of a FGF-17 or a modified .gamma.-fold
motif of a .beta.-trefoil domain of a FGF-17 of SEQ ID NO: 38. In
another aspect of this embodiment, a .beta.-trefoil domain with
enhanced binding activity derived from a FGF-17 comprises amino
acids 55-91, amino acids 95-134, or amino acids 138-183 of SEQ ID
NO: 38.
[0667] In other aspects of this embodiment, a FGF-17 comprising a
.beta.-trefoil domain with enhanced binding activity comprises a
polypeptide having, e.g., at least 70% amino acid identity with
amino acids 55-91, amino acids 95-134, or amino acids 138-183 of
SEQ ID NO: 38, at least 75% amino acid identity with amino acids
55-91, amino acids 95-134, or amino acids 138-183 of SEQ ID NO: 38,
at least 80% amino acid identity with amino acids 55-91, amino
acids 95-134, or amino acids 138-183 of SEQ ID NO: 38, at least 85%
amino acid identity with amino acids 55-91, amino acids 95-134, or
amino acids 138-183 of SEQ ID NO: 38, at least 90% amino acid
identity with amino acids 55-91, amino acids 95-134, or amino acids
138-183 of SEQ ID NO: 38 or at least 95% amino acid identity with
amino acids 55-91, amino acids 95-134, or amino acids 138-183 of
SEQ ID NO: 38. In yet other aspects of this embodiment, a FGF-17
comprising a .beta.-trefoil domain with enhanced binding activity
comprises a polypeptide having, e.g., at most 70% amino acid
identity with amino acids 55-91, amino acids 95-134, or amino acids
138-183 of SEQ ID NO: 38, at most 75% amino acid identity with
amino acids 55-91, amino acids 95-134, or amino acids 138-183 of
SEQ ID NO: 38, at most 80% amino acid identity with amino acids
55-91, amino acids 95-134, or amino acids 138-183 of SEQ ID NO: 38,
at most 85% amino acid identity with amino acids 55-91, amino acids
95-134, or amino acids 138-183 of SEQ ID NO: 38, at most 90% amino
acid identity with amino acids 55-91, amino acids 95-134, or amino
acids 138-183 of SEQ ID NO: 38 or at most 95% amino acid identity
with amino acids 55-91, amino acids 95-134, or amino acids 138-183
of SEQ ID NO: 38.
[0668] In other aspects of this embodiment, a FGF-17 comprising a
.beta.-trefoil domain with enhanced binding activity comprises a
polypeptide having, e.g., at most one, two, three, four, five, six,
seven, eight, nine, 10 or 20 non-contiguous amino acid
substitutions relative to amino acids 55-91, amino acids 95-134, or
amino acids 138-183 of SEQ ID NO: 38. In other aspects of this
embodiment, a FGF-17 comprising a .beta.-trefoil domain with
enhanced binding activity comprises a polypeptide having, e.g., at
least one, two, three, four, five, six, seven, eight, nine, 10 or
20 non-contiguous amino acid substitutions relative to amino acids
55-91, amino acids 95-134, or amino acids 138-183 of SEQ ID NO: 38.
In yet other aspects of this embodiment, a FGF-17 comprising a
.beta.-trefoil domain with enhanced binding activity comprises a
polypeptide having, e.g., at most one, two, three, four, five, six,
seven, eight, nine, 10 or 20 non-contiguous amino acid deletions
relative to amino acids 55-91, amino acids 95-134, or amino acids
138-183 of SEQ ID NO: 38. In other aspects of this embodiment, a
FGF-17 comprising a .beta.-trefoil domain with enhanced binding
activity comprises a polypeptide having, e.g., at least one, two,
three, four, five, six, seven, eight, nine, 10 or 20 non-contiguous
amino acid deletions relative to amino acids 55-91, amino acids
95-134, or amino acids 138-183 of SEQ ID NO: 38. In still other
aspects of this embodiment, a FGF-17 comprising a .beta.-trefoil
domain with enhanced binding activity comprises a polypeptide
having, e.g., at most one, two, three, four, five, six, seven,
eight, nine, 10 or 20 non-contiguous amino acid additions relative
to amino acids 55-91, amino acids 95-134, or amino acids 138-183 of
SEQ ID NO: 38. In other aspects of this embodiment, a FGF-17
comprising a .beta.-trefoil domain with enhanced binding activity
comprises a polypeptide having, e.g., at least one, two, three,
four, five, six, seven, eight, nine, 10 or 20 non-contiguous amino
acid additions relative to amino acids 55-91, amino acids 95-134,
or amino acids 138-183 of SEQ ID NO: 38.
[0669] In other aspects of this embodiment, a FGF-17 comprising a
.beta.-trefoil domain with enhanced binding activity comprises a
polypeptide having, e.g., at most one, two, three, four, five, six,
seven, eight, nine, 10 or 20 contiguous amino acid substitutions
relative to amino acids 55-91, amino acids 95-134, or amino acids
138-183 of SEQ ID NO: 38. In other aspects of this embodiment, a
FGF-17 comprising a .beta.-trefoil domain with enhanced binding
activity comprises a polypeptide having, e.g., at least one, two,
three, four, five, six, seven, eight, nine, 10 or 20 contiguous
amino acid substitutions relative to amino acids 55-91, amino acids
95-134, or amino acids 138-183 of SEQ ID NO: 38. In yet other
aspects of this embodiment, a FGF-17 comprising a .beta.-trefoil
domain with enhanced binding activity comprises a polypeptide
having, e.g., at most one, two, three, four, five, six, seven,
eight, nine, 10 or 20 contiguous amino acid deletions relative to
amino acids 55-91, amino acids 95-134, or amino acids 138-183 of
SEQ ID NO: 38. In other aspects of this embodiment, a FGF-17
comprising a .beta.-trefoil domain with enhanced binding activity
comprises a polypeptide having, e.g., at least one, two, three,
four, five, six, seven, eight, nine, 10 or 20 contiguous amino acid
deletions relative to amino acids 55-91, amino acids 95-134, or
amino acids 138-183 of SEQ ID NO: 38. In still other aspects of
this embodiment, a FGF-17 comprising a .beta.-trefoil domain with
enhanced binding activity comprises a polypeptide having, e.g., at
most one, two, three, four, five, six, seven, eight, nine, 10 or 20
contiguous amino acid additions relative to amino acids 55-91,
amino acids 95-134, or amino acids 138-183 of SEQ ID NO: 38. In
other aspects of this embodiment, a FGF-17 comprising a
.beta.-trefoil domain with enhanced binding activity comprises a
polypeptide having, e.g., at least one, two, three, four, five,
six, seven, eight, nine, 10 or 20 contiguous amino acid additions
relative to amino acids 55-91, amino acids 95-134, or amino acids
138-183 of SEQ ID NO: 38.
[0670] In another embodiment, a .beta.-trefoil domain derived from
a FGF-17 comprises a .beta.4/.beta.5 hairpin turn of a
.beta.-trefoil domain of a FGF-17 or a .beta.8/.beta.9 hairpin turn
of a .beta.-trefoil domain of a FGF-17 of SEQ ID NO: 38. In another
aspect of this embodiment, a .beta.-trefoil domain derived from a
FGF-17 comprises amino acids 92-94 or amino acids 135-137 of SEQ ID
NO: 38.
[0671] In another embodiment, a .beta.-trefoil domain with enhanced
binding activity derived from a FGF-17 comprises a modified
.beta.4/.beta.5 hairpin turn of a .beta.-trefoil domain of a FGF-17
or a modified .beta.8/.beta.9 hairpin turn of a .beta.-trefoil
domain of a FGF-17 of SEQ ID NO: 38. In another aspect of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a FGF-17 comprises a modification of amino acids 92-94
or amino acids 135-137 of SEQ ID NO: 38.
[0672] In other aspects of this embodiment, a .beta.-trefoil domain
with enhanced binding activity derived from a FGF-17 comprises a
polypeptide having, e.g., at least 70% amino acid identity with
amino acids 92-94 or amino acids 135-137 of SEQ ID NO: 38, at least
75% amino acid identity with amino acids 92-94 or amino acids
135-137 of SEQ ID NO: 38, at least 80% amino acid identity with
amino acids 92-94 or amino acids 135-137 of SEQ ID NO: 38, at least
85% amino acid identity with amino acids 92-94 or amino acids
135-137 of SEQ ID NO: 38, at least 90% amino acid identity with
amino acids 92-94 or amino acids 135-137 of SEQ ID NO: 38 or at
least 95% amino acid identity with amino acids 92-94 or amino acids
135-137 of SEQ ID NO: 38. In yet other aspects of this embodiment,
a .beta.-trefoil domain with enhanced binding activity derived from
a FGF-17 comprises a polypeptide having, e.g., at most 70% amino
acid identity with amino acids 92-94 or amino acids 135-137 of SEQ
ID NO: 38, at most 75% amino acid identity with amino acids 92-94
or amino acids 135-137 of SEQ ID NO: 38, at most 80% amino acid
identity with amino acids 92-94 or amino acids 135-137 of SEQ ID
NO: 38, at most 85% amino acid identity with amino acids 92-94 or
amino acids 135-137 of SEQ ID NO: 38, at most 90% amino acid
identity with amino acids 92-94 or amino acids 135-137 of SEQ ID
NO: 38 or at most 95% amino acid identity with amino acids 92-94 or
amino acids 135-137 of SEQ ID NO: 38.
[0673] In other aspects of this embodiment, a .beta.-trefoil domain
with enhanced binding activity derived from a FGF-17 comprises a
polypeptide having, e.g., at most one, two, three or four
non-contiguous amino acid substitutions relative to amino acids
92-94 or amino acids 135-137 of SEQ ID NO: 38. In other aspects of
this embodiment, a .beta.-trefoil domain with enhanced binding
activity derived from a FGF-17 comprises a polypeptide having,
e.g., at least one, two, three or four non-contiguous amino acid
substitutions relative to amino acids 92-94 or amino acids 135-137
of SEQ ID NO: 38. In other aspects of this embodiment, a
non-contiguous amino acid substitution of any amino acid from amino
acids 92-94 or amino acids 135-137 of SEQ ID NO: 38 can be replaced
with glycine. In other aspects of this embodiment, a non-contiguous
amino acid substitution of any hydrophobic amino acid from amino
acids 92-94 or amino acids 135-137 of SEQ ID NO: 38 can be replaced
with phenylalanine. In yet other aspects of this embodiment, a
.beta.-trefoil domain with enhanced binding activity derived from a
FGF-17 comprises a polypeptide having, e.g., at most one, two,
three or four non-contiguous amino acid deletions relative to amino
acids 92-94 or amino acids 135-137 of SEQ ID NO: 38. In other
aspects of this embodiment, a .beta.-trefoil domain with enhanced
binding activity derived from a FGF-17 comprises a polypeptide
having, e.g., at least one, two, three or four non-contiguous amino
acid deletions relative to amino acids 92-94 or amino acids 135-137
of SEQ ID NO: 38. In still other aspects of this embodiment, a
.beta.-trefoil domain with enhanced binding activity derived from a
FGF-17 comprises a polypeptide having, e.g., at most one, two,
three or four non-contiguous amino acid additions relative to amino
acids 92-94 or amino acids 135-137 of SEQ ID NO: 38. In other
aspects of this embodiment, a .beta.-trefoil domain with enhanced
binding activity derived from a FGF-17 comprises a polypeptide
having, e.g., at least one, two, three or four non-contiguous amino
acid additions relative to amino acids 92-94 or amino acids 135-137
of SEQ ID NO: 38.
[0674] In other aspects of this embodiment, a .beta.-trefoil domain
with enhanced binding activity derived from a FGF-17 comprises a
polypeptide having, e.g., at most one, two, three or four
contiguous amino acid substitutions relative to amino acids 92-94
or amino acids 135-137 of SEQ ID NO: 38. In other aspects of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a FGF-17 comprises a polypeptide having, e.g., at
least one, two, three or four contiguous amino acid substitutions
relative to amino acids 92-94 or amino acids 135-137 of SEQ ID NO:
38. In other aspects of this embodiment, contiguous amino acid
substitutions of amino acids from amino acids 92-94 or amino acids
135-137 of SEQ ID NO: 38 can be replaced with glycine. In other
aspects of this embodiment, contiguous amino acid substitutions of
hydrophobic amino acids from amino acids 92-94 or amino acids
135-137 of SEQ ID NO: 38 can be replaced with phenylalanine. In yet
other aspects of this embodiment, a .beta.-trefoil domain with
enhanced binding activity derived from a FGF-17 comprises a
polypeptide having, e.g., at most one, two, three or four
contiguous amino acid deletions relative to amino acids 92-94 or
amino acids 135-137 of SEQ ID NO: 38. In other aspects of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a FGF-17 comprises a polypeptide having, e.g., at
least one, two, three or four contiguous amino acid deletions
relative to amino acids 92-94 or amino acids 135-137 of SEQ ID NO:
38. In still other aspects of this embodiment, a .beta.-trefoil
domain with enhanced binding activity derived from a FGF-17
comprises a polypeptide having, e.g., at most one, two, three or
four contiguous amino acid additions relative to amino acids 92-94
or amino acids 135-137 of SEQ ID NO: 38. In other aspects of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a FGF-17 comprises a polypeptide having, e.g., at
least one, two, three or four contiguous amino acid additions
relative to amino acids 92-94 or amino acids 135-137 of SEQ ID NO:
38.
[0675] In another embodiment, a .beta.-trefoil domain derived from
a FGF-18 comprises a .beta.-trefoil domain derived from a FGF-18 of
SEQ ID NO: 39. In another embodiment, a .beta.-trefoil domain
derived from a FGF-18 comprises amino acids 54-183 of SEQ ID NO:
39. In another aspect of this embodiment, a .beta.-trefoil domain
derived from a FGF-18 comprises a .alpha.-fold motif of a
.beta.-trefoil domain of a FGF-18, a .beta.-fold motif of a
.beta.-trefoil domain of a FGF-18 or a .gamma.-fold motif of a
.beta.-trefoil domain of a FGF-18 of SEQ ID NO: 39. In another
aspect of this embodiment, a .beta.-trefoil domain derived from a
FGF-18 comprises amino acids 54-91, amino acids 95-134, or amino
acids 138-183 of SEQ ID NO: 39.
[0676] In another embodiment, a .beta.-trefoil domain with enhanced
binding activity derived from a FGF-18 comprises a .beta.-trefoil
domain with enhanced binding activity derived from a FGF-18 of SEQ
ID NO: 39. In another embodiment, a .beta.-trefoil domain with
enhanced binding activity derived from a FGF-18 comprises amino
acids 54-183 of SEQ ID NO: 39. In another aspect of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a FGF-18 comprises a modified .alpha.-fold motif of a
.beta.-trefoil domain of a FGF-18, a modified .beta.-fold motif of
a .beta.-trefoil domain of a FGF-18 or a modified .gamma.-fold
motif of a .beta.-trefoil domain of a FGF-18 of SEQ ID NO: 39. In
another aspect of this embodiment, a .beta.-trefoil domain with
enhanced binding activity derived from a FGF-18 comprises amino
acids 54-91, amino acids 95-134, or amino acids 138-183 of SEQ ID
NO: 39.
[0677] In other aspects of this embodiment, a FGF-18 comprising a
.beta.-trefoil domain with enhanced binding activity comprises a
polypeptide having, e.g., at least 70% amino acid identity with
amino acids 54-91, amino acids 95-134, or amino acids 138-183 of
SEQ ID NO: 39, at least 75% amino acid identity with amino acids
54-91, amino acids 95-134, or amino acids 138-183 of SEQ ID NO: 39,
at least 80% amino acid identity with amino acids 54-91, amino
acids 95-134, or amino acids 138-183 of SEQ ID NO: 39, at least 85%
amino acid identity with amino acids 54-91, amino acids 95-134, or
amino acids 138-183 of SEQ ID NO: 39, at least 90% amino acid
identity with amino acids 54-91, amino acids 95-134, or amino acids
138-183 of SEQ ID NO: 39 or at least 95% amino acid identity with
amino acids 54-91, amino acids 95-134, or amino acids 138-183 of
SEQ ID NO: 39. In yet other aspects of this embodiment, a FGF-18
comprising a .beta.-trefoil domain with enhanced binding activity
comprises a polypeptide having, e.g., at most 70% amino acid
identity with amino acids 54-91, amino acids 95-134, or amino acids
138-183 of SEQ ID NO: 39, at most 75% amino acid identity with
amino acids 54-91, amino acids 95-134, or amino acids 138-183 of
SEQ ID NO: 39, at most 80% amino acid identity with amino acids
54-91, amino acids 95-134, or amino acids 138-183 of SEQ ID NO: 39,
at most 85% amino acid identity with amino acids 54-91, amino acids
95-134, or amino acids 138-183 of SEQ ID NO: 39, at most 90% amino
acid identity with amino acids 54-91, amino acids 95-134, or amino
acids 138-183 of SEQ ID NO: 39 or at most 95% amino acid identity
with amino acids 54-91, amino acids 95-134, or amino acids 138-183
of SEQ ID NO: 39.
[0678] In other aspects of this embodiment, a FGF-18 comprising a
.beta.-trefoil domain with enhanced binding activity comprises a
polypeptide having, e.g., at most one, two, three, four, five, six,
seven, eight, nine, 10 or 20 non-contiguous amino acid
substitutions relative to amino acids 54-91, amino acids 95-134, or
amino acids 138-183 of SEQ ID NO: 39. In other aspects of this
embodiment, a FGF-18 comprising a .beta.-trefoil domain with
enhanced binding activity comprises a polypeptide having, e.g., at
least one, two, three, four, five, six, seven, eight, nine, 10 or
20 non-contiguous amino acid substitutions relative to amino acids
54-91, amino acids 95-134, or amino acids 138-183 of SEQ ID NO: 39.
In yet other aspects of this embodiment, a FGF-18 comprising a
.beta.-trefoil domain with enhanced binding activity comprises a
polypeptide having, e.g., at most one, two, three, four, five, six,
seven, eight, nine, 10 or 20 non-contiguous amino acid deletions
relative to amino acids 54-91, amino acids 95-134, or amino acids
138-183 of SEQ ID NO: 39. In other aspects of this embodiment, a
FGF-18 comprising a .beta.-trefoil domain with enhanced binding
activity comprises a polypeptide having, e.g., at least one, two,
three, four, five, six, seven, eight, nine, 10 or 20 non-contiguous
amino acid deletions relative to amino acids 54-91, amino acids
95-134, or amino acids 138-183 of SEQ ID NO: 39. In still other
aspects of this embodiment, a FGF-18 comprising a .beta.-trefoil
domain with enhanced binding activity comprises a polypeptide
having, e.g., at most one, two, three, four, five, six, seven,
eight, nine, 10 or 20 non-contiguous amino acid additions relative
to amino acids 54-91, amino acids 95-134, or amino acids 138-183 of
SEQ ID NO: 39. In other aspects of this embodiment, a FGF-18
comprising a .beta.-trefoil domain with enhanced binding activity
comprises a polypeptide having, e.g., at least one, two, three,
four, five, six, seven, eight, nine, 10 or 20 non-contiguous amino
acid additions relative to amino acids 54-91, amino acids 95-134,
or amino acids 138-183 of SEQ ID NO: 39.
[0679] In other aspects of this embodiment, a FGF-18 comprising a
.beta.-trefoil domain with enhanced binding activity comprises a
polypeptide having, e.g., at most one, two, three, four, five, six,
seven, eight, nine, 10 or 20 contiguous amino acid substitutions
relative to amino acids 54-91, amino acids 95-134, or amino acids
138-183 of SEQ ID NO: 39. In other aspects of this embodiment, a
FGF-18 comprising a 11-trefoil domain with enhanced binding
activity comprises a polypeptide having, e.g., at least one, two,
three, four, five, six, seven, eight, nine, 10 or 20 contiguous
amino acid substitutions relative to amino acids 54-91, amino acids
95-134, or amino acids 138-183 of SEQ ID NO: 39. In yet other
aspects of this embodiment, a FGF-18 comprising a .beta.-trefoil
domain with enhanced binding activity comprises a polypeptide
having, e.g., at most one, two, three, four, five, six, seven,
eight, nine, 10 or 20 contiguous amino acid deletions relative to
amino acids 54-91, amino acids 95-134, or amino acids 138-183 of
SEQ ID NO: 39. In other aspects of this embodiment, a FGF-18
comprising a .beta.-trefoil domain with enhanced binding activity
comprises a polypeptide having, e.g., at least one, two, three,
four, five, six, seven, eight, nine, 10 or 20 contiguous amino acid
deletions relative to amino acids 54-91, amino acids 95-134, or
amino acids 138-183 of SEQ ID NO: 39. In still other aspects of
this embodiment, a FGF-18 comprising a .beta.-trefoil domain with
enhanced binding activity comprises a polypeptide having, e.g., at
most one, two, three, four, five, six, seven, eight, nine, 10 or 20
contiguous amino acid additions relative to amino acids 54-91,
amino acids 95-134, or amino acids 138-183 of SEQ ID NO: 39. In
other aspects of this embodiment, a FGF-18 comprising a
.beta.-trefoil domain with enhanced binding activity comprises a
polypeptide having, e.g., at least one, two, three, four, five,
six, seven, eight, nine, 10 or 20 contiguous amino acid additions
relative to amino acids 54-91, amino acids 95-134, or amino acids
138-183 of SEQ ID NO: 39.
[0680] In another embodiment, a .beta.-trefoil domain derived from
a FGF-18 comprises a .beta.4/.beta.5 hairpin turn of a
.beta.-trefoil domain of a FGF-18 or a .beta.8/.beta.9 hairpin turn
of a .beta.-trefoil domain of a FGF-18 of SEQ ID NO: 39. In another
aspect of this embodiment, a .beta.-trefoil domain derived from a
FGF-18 comprises amino acids 92-94 or amino acids 135-137 of SEQ ID
NO: 39.
[0681] In another embodiment, a .beta.-trefoil domain with enhanced
binding activity derived from a FGF-18 comprises a modified
.beta.4/.beta.5 hairpin turn of a .beta.-trefoil domain of a FGF-18
or a modified .beta.8/.beta.9 hairpin turn of a .beta.-trefoil
domain of a FGF-18 of SEQ ID NO: 39. In another aspect of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a FGF-18 comprises a modification of amino acids 92-94
or amino acids 135-137 of SEQ ID NO: 39.
[0682] In other aspects of this embodiment, a .beta.-trefoil domain
with enhanced binding activity derived from a FGF-18 comprises a
polypeptide having, e.g., at least 70% amino acid identity with
amino acids 92-94 or amino acids 135-137 of SEQ ID NO: 39, at least
75% amino acid identity with amino acids 92-94 or amino acids
135-137 of SEQ ID NO: 39, at least 80% amino acid identity with
amino acids 92-94 or amino acids 135-137 of SEQ ID NO: 39, at least
85% amino acid identity with amino acids 92-94 or amino acids
135-137 of SEQ ID NO: 39, at least 90% amino acid identity with
amino acids 92-94 or amino acids 135-137 of SEQ ID NO: 39 or at
least 95% amino acid identity with amino acids 92-94 or amino acids
135-137 of SEQ ID NO: 39. In yet other aspects of this embodiment,
a .beta.-trefoil domain with enhanced binding activity derived from
a FGF-18 comprises a polypeptide having, e.g., at most 70% amino
acid identity with amino acids 92-94 or amino acids 135-137 of SEQ
ID NO: 39, at most 75% amino acid identity with amino acids 92-94
or amino acids 135-137 of SEQ ID NO: 39, at most 80% amino acid
identity with amino acids 92-94 or amino acids 135-137 of SEQ ID
NO: 39, at most 85% amino acid identity with amino acids 92-94 or
amino acids 135-137 of SEQ ID NO: 39, at most 90% amino acid
identity with amino acids 92-94 or amino acids 135-137 of SEQ ID
NO: 39 or at most 95% amino acid identity with amino acids 92-94 or
amino acids 135-137 of SEQ ID NO: 39.
[0683] In other aspects of this embodiment, a .beta.-trefoil domain
with enhanced binding activity derived from a FGF-18 comprises a
polypeptide having, e.g., at most one, two, three or four
non-contiguous amino acid substitutions relative to amino acids
92-94 or amino acids 135-137 of SEQ ID NO: 39. In other aspects of
this embodiment, a .beta.-trefoil domain with enhanced binding
activity derived from a FGF-18 comprises a polypeptide having,
e.g., at least one, two, three or four non-contiguous amino acid
substitutions relative to amino acids 92-94 or amino acids 135-137
of SEQ ID NO: 39. In other aspects of this embodiment, a
non-contiguous amino acid substitution of any amino acid from amino
acids 92-94 or amino acids 135-137 of SEQ ID NO: 39 can be replaced
with glycine. In other aspects of this embodiment, a non-contiguous
amino acid substitution of any hydrophobic amino acid from amino
acids 92-94 or amino acids 135-137 of SEQ ID NO: 39 can be replaced
with phenylalanine. In yet other aspects of this embodiment, a
.beta.-trefoil domain with enhanced binding activity derived from a
FGF-18 comprises a polypeptide having, e.g., at most one, two,
three or four non-contiguous amino acid deletions relative to amino
acids 92-94 or amino acids 135-137 of SEQ ID NO: 39. In other
aspects of this embodiment, a .beta.-trefoil domain with enhanced
binding activity derived from a FGF-18 comprises a polypeptide
having, e.g., at least one, two, three or four non-contiguous amino
acid deletions relative to amino acids 92-94 or amino acids 135-137
of SEQ ID NO: 39. In still other aspects of this embodiment, a
.beta.-trefoil domain with enhanced binding activity derived from a
FGF-18 comprises a polypeptide having, e.g., at most one, two,
three or four non-contiguous amino acid additions relative to amino
acids 92-94 or amino acids 135-137 of SEQ ID NO: 39. In other
aspects of this embodiment, a .beta.-trefoil domain with enhanced
binding activity derived from a FGF-18 comprises a polypeptide
having, e.g., at least one, two, three or four non-contiguous amino
acid additions relative to amino acids 92-94 or amino acids 135-137
of SEQ ID NO: 39.
[0684] In other aspects of this embodiment, a .beta.-trefoil domain
with enhanced binding activity derived from a FGF-18 comprises a
polypeptide having, e.g., at most one, two, three or four
contiguous amino acid substitutions relative to amino acids 92-94
or amino acids 135-137 of SEQ ID NO: 39. In other aspects of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a FGF-18 comprises a polypeptide having, e.g., at
least one, two, three or four contiguous amino acid substitutions
relative to amino acids 92-94 or amino acids 135-137 of SEQ ID NO:
39. In other aspects of this embodiment, contiguous amino acid
substitutions of amino acids from amino acids 92-94 or amino acids
135-137 of SEQ ID NO: 39 can be replaced with glycine. In other
aspects of this embodiment, contiguous amino acid substitutions of
hydrophobic amino acids from amino acids 92-94 or amino acids
135-137 of SEQ ID NO: 39 can be replaced with phenylalanine. In yet
other aspects of this embodiment, a .beta.-trefoil domain with
enhanced binding activity derived from a FGF-18 comprises a
polypeptide having, e.g., at most one, two, three or four
contiguous amino acid deletions relative to amino acids 92-94 or
amino acids 135-137 of SEQ ID NO: 39. In other aspects of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a FGF-18 comprises a polypeptide having, e.g., at
least one, two, three or four contiguous amino acid deletions
relative to amino acids 92-94 or amino acids 135-137 of SEQ ID NO:
39. In still other aspects of this embodiment, a .beta.-trefoil
domain with enhanced binding activity derived from a FGF-18
comprises a polypeptide having, e.g., at most one, two, three or
four contiguous amino acid additions relative to amino acids 92-94
or amino acids 135-137 of SEQ ID NO: 39. In other aspects of this
embodiment, a .beta.-trefoil domain with enhanced binding activity
derived from a FGF-18 comprises a polypeptide having, e.g., at
least one, two, three or four contiguous amino acid additions
relative to amino acids 92-94 or amino acids 135-137 of SEQ ID NO:
39.
[0685] Because of the modular nature of the .beta.-trefoil domain,
it is envisioned that any combination of .alpha.-folds,
.beta.-folds, .gamma.-folds, .beta.4/.beta.5 .beta.-hairpin turns,
.beta.8/.beta.9 .beta.-hairpin turns, or any combination thereof
from a .beta.-trefoil domain of a Clostridial toxin binding domain,
a .beta.-trefoil domain of a modified Clostridial toxin binding
domain, a .beta.-trefoil domain of a NAP or a .beta.-trefoil
domain, a .beta.-trefoil domain of a modified NAP, a .beta.-trefoil
domain of an FGF ligand, a .beta.-trefoil domain of a modified FGF
ligand, or any combination thereof, can be used to practice aspect
of the present invention. As a non-limiting example, a modified
Clostridial toxin disclosed in the present specification can
comprise an enhanced targeting domain comprising a .alpha.-fold
from a BoNT/A binding domain, a .beta.-fold from a FGF-18 and a
2.gamma.-fold from a Clostridial botulinum serotype A HA-33. As
another non-limiting example, a modified Clostridial toxin
disclosed in the present specification can comprise an enhanced
targeting domain comprising a .alpha.-fold from a Clostridial
botulinum serotype A HA-17, a .beta.-fold from a Clostridial
botulinum serotype E NTNH and a .gamma.-fold from a modified
BoNT/C1 binding domain with enhanced binding activity.
[0686] In another aspect of the invention, a modified Clostridial
toxin with enhanced binding activity comprises, in part, a protease
cleavage site is located within a di-chain loop region. As used
herein, the term "di-chain loop region" means the amino acid
sequence of a Clostridial toxin containing a protease cleavage site
used to convert the single-chain form of a Clostridial toxin into
the di-chain form. Non-limiting examples of a Clostridial toxin
di-chain loop region, include, a di-chain loop region of BoNT/A
comprising amino acids 430-454 of SEQ ID NO: 1; a di-chain loop
region of BoNT/B comprising amino acids 437-446 of SEQ ID NO: 2; a
di-chain loop region of BoNT/C1 comprising amino acids 437-453 of
SEQ ID NO: 3; a di-chain loop region of BoNT/D comprising amino
acids 437-450 of SEQ ID NO: 4; a di-chain loop region of BoNT/E
comprising amino acids 412-426 of SEQ ID NO: 5; a di-chain loop
region of BoNT/F comprising amino acids 429-445 of SEQ ID NO: 6; a
di-chain loop region of BoNT/G comprising amino acids 436-450 of
SEQ ID NO: 7; and a di-chain loop region of TeNT comprising amino
acids 439-467 of SEQ ID NO: 8 (Table 8).
TABLE-US-00008 TABLE 8 Di-chain Loop Region of Clostridial Toxins
SEQ Di-chain Loop Region Containing ID Light Chain the
Naturally-occurring Heavy Chain Toxin NO: Region Protease Cleavage
Site Region BoNT/A 1 NMNFTKLKNFTGLFEFYKLL
CVRGIITSKTKSLDKGYNK*----ALNDLC IKVNNWDL BoNT/B 2
KQAYEEISKEHLAVYKIQM CKSVK*-------------------APGIC IDVDNEDL BoNT/C1
3 PALRKVNPENMLYLFTKF CHKAIDGRSLYNK*------------TLDC RELLVKNTDL
BoNT/D 4 PALQKLSSESVVDLFTKV CLRLTKNSR*---------------DDSTC IKVKNNRL
BoNT/E 5 IITPITGRGLVKKIIRF CKNIVSVKGIR*--------------KSIC IEINNGEL
BoNT/F 6 IIDSIPDKGLVEKIVKF CKSVIPRKGTK*------------APPRLC IRVNNSEL
BoNT/G 7 KEAYEEISLEHLVIYRIAM CKPVMYKNTGK*--------------SEQC
IIVNNEDL TeNT 8 TNAFRNVDGSGLVSKLIGL CKKIIPPTNIRENLYNRTA*SLTDLGGELC
IKIKNEDL The amino acid sequence displayed are as follows: BoNT/A,
residues 325-462 of SEQ ID No: 1; BoNT/B, residues 332-454 of SEQ
ID No: 2; BoNT/C1, residues 334-463 of SEQ ID No: 3; BoNT/D,
residues 334-458 of SEQ ID No: 4; BoNT/E, residues 311-434 of SEQ
ID No: 5; BoNT/F, residues 328-453 of SEQ ID No: 6; BoNT/G,
residues 331-458 of SEQ ID No: 7; and TeNT, residues 334-474 of SEQ
ID No: 8. An asterisks (*) indicates a peptide bond that is cleaved
by a Clostridial toxin protease.
[0687] In is envisioned that any and all protease cleavage sites
can be used to convert the single-chain polypeptide form of a
Clostridial toxin into the di-chain form, including, without
limitation, endogenous di-chain loop protease cleavage sites and
exogenous protease cleavage sites. The location and kind of
protease cleavage site may be critical because certain targeting
domains require a free amino-terminal or carboxyl-terminal amino
acid. For example, when a targeting domain is placed between two
other domains, e.g., see FIG. 5, a criteria for selection of a
protease cleavage site could be whether the protease that cleaves
its site leaves a flush cut, exposing the free amino-terminal or
carboxyl-terminal of the altered targeting domain necessary for
selective binding of the targeting domain to its receptor. The
selection and placement of a protease cleavage site is well known
in the art.
[0688] As used herein, the term "endogenous di-chain loop protease
cleavage site" is synonymous with a "naturally occurring di-chain
loop protease cleavage site" and means a naturally occurring
protease cleavage site found within the di-chain loop region of a
naturally occurring Clostridial toxin and includes, without
limitation, naturally occurring Clostridial toxin di-chain loop
protease cleavage site variants, such as, e.g., Clostridial toxin
di-chain loop protease cleavage site isoforms and Clostridial toxin
di-chain loop protease cleavage site subtypes. Non-limiting
examples of an endogenous protease cleavage site, include, e.g., a
BoNT/A di-chain loop protease cleavage site, a BoNT/B di-chain loop
protease cleavage site, a BoNT/C1 di-chain loop protease cleavage
site, a BoNT/D di-chain loop protease cleavage site, a BoNT/E
di-chain loop protease cleavage site, a BoNT/F di-chain loop
protease cleavage site, a BoNT/G di-chain loop protease cleavage
site and a TeNT di-chain loop protease cleavage site.
[0689] As mentioned above, Clostridial toxins are translated as a
single-chain polypeptide of approximately 150 kDa that is
subsequently cleaved by proteolytic scission within a disulfide
loop by a naturally-occurring protease. This posttranslational
processing yields a di-chain molecule comprising an approximately
50 kDa light chain (LC) and an approximately 100 kDa heavy chain
(HC) held together by a single disulphide bond and noncovalent
interactions. While the identity of the protease is currently
unknown, the di-chain loop protease cleavage site for many
Clostridial toxins has been proposed. In BoNTs, cleavage at
K448-A449 converts the single polypeptide form of BoNT/A into the
di-chain form; cleavage at K441-A442 converts the single
polypeptide form of BoNT/B into the di-chain form; cleavage at
K449-T450 converts the single polypeptide form of BoNT/C1 into the
di-chain form; cleavage at R445-D446 converts the single
polypeptide form of BoNT/D into the di-chain form; cleavage at
R422-K423 converts the single polypeptide form of BoNT/E into the
di-chain form; cleavage at K439-A440 converts the single
polypeptide form of BoNT/F into the di-chain form; and cleavage at
K446-5447 converts the single polypeptide form of BoNT/G into the
di-chain form. Proteolytic cleavage of the single polypeptide form
of TeNT at A457-5458 results in the di-chain form. Such a di-chain
loop protease cleavage site is operably-linked in-frame to a
modified Clostridial toxin as a fusion protein.
[0690] However, it should also be noted that additional cleavage
sites within the di-chain loop also appear to be cleaved resulting
in the generation of a small peptide fragment being lost. As a
non-limiting example, BoNT/A single-chain polypeptide cleave
ultimately results in the loss of a ten amino acid fragment within
the di-chain loop. Thus, in BoNTs, cleavage at 5441-L442 converts
the single polypeptide form of BoNT/A into the di-chain form;
cleavage at G444-I445 converts the single polypeptide form of
BoNT/B into the di-chain form; cleavage at S445-L446 converts the
single polypeptide form of BoNT/C1 into the di-chain form; cleavage
at K442-N443 converts the single polypeptide form of BoNT/D into
the di-chain form; cleavage at K419-G420 converts the single
polypeptide form of BoNT/E into the di-chain form; cleavage at
K423-5424 converts the single polypeptide form of BoNT/E into the
di-chain form; cleavage at K436-G437 converts the single
polypeptide form of BoNT/F into the di-chain form; cleavage at
T444-G445 converts the single polypeptide form of BoNT/G into the
di-chain form; and cleavage at E448-Q449 converts the single
polypeptide form of BoNT/G into the di-chain form.
[0691] Thus, in an embodiment, a protease cleavage site comprising
an endogenous Clostridial toxin di-chain loop protease cleavage
site is used to convert the single-chain toxin into the di-chain
form. In aspects of this embodiment, conversion into the di-chain
form by proteolytic cleavage occurs from a site comprising, e.g., a
BoNT/A di-chain loop protease cleavage site, a BoNT/B di-chain loop
protease cleavage site, a BoNT/C1 di-chain loop protease cleavage
site, a BoNT/D di-chain loop protease cleavage site, a BoNT/E
di-chain loop protease cleavage site, a BoNT/F di-chain loop
protease cleavage site, a BoNT/G di-chain loop protease cleavage
site or a TeNT di-chain loop protease cleavage site.
[0692] In other aspects of this embodiment, conversion into the
di-chain form by proteolytic cleavage occurs from a site
comprising, e.g., a di-chain loop region of BoNT/A comprising amino
acids 430-454 of SEQ ID NO: 1; a di-chain loop region of BoNT/B
comprising amino acids 437-446 of SEQ ID NO: 2; a di-chain loop
region of BoNT/C1 comprising amino acids 437-453 of SEQ ID NO: 3; a
di-chain loop region of BoNT/D comprising amino acids 437-450 of
SEQ ID NO: 4; a di-chain loop region of BoNT/E comprising amino
acids 412-426 of SEQ ID NO: 5; a di-chain loop region of BoNT/F
comprising amino acids 429-445 of SEQ ID NO: 6; a di-chain loop
region of BoNT/G comprising amino acids 436-450 of SEQ ID NO: 7; or
a di-chain loop region of TeNT comprising amino acids 439-467 of
SEQ ID NO: 8.
[0693] It is also envisioned that an exogenous protease cleavage
site can be used to convert the single-chain polypeptide form of a
modified Clostridial toxin disclosed in the present specification
into the di-chain form. As used herein, the term "exogenous
protease cleavage site" is synonymous with a "non-naturally
occurring protease cleavage site" and means a protease cleavage
site that is not normally present in a di-chain loop region from a
naturally occurring Clostridial toxin. Non-limiting examples of
exogenous protease cleavage sites include, e.g., an enterokinase
cleavage site (Table 9); a Thrombin cleavage site (Table 9); a
Factor Xa cleavage site (Table 9); a human rhinovirus 3C protease
cleavage site (Table 9); a tobacco etch virus (TEV) protease
cleavage site (Table 9); a dipeptidyl aminopeptidase cleavage site;
a small ubiquitin-like modifier (SUMO)/ubiquitin-like protein-1
(ULP-1) protease cleavage site, such as, e.g.,
MADSEVNQEAKPEVKPEVKPETH INLKVSDGSSEIFFKIKKTTPLRRLMEAFAKRQGK
EMDSLRFLYDGIRIQADQTPEDLDMEDNDIIEAHREQIGG (SEQ ID. NO: 57); and a
Clostridial toxin substrate cleavage site.
TABLE-US-00009 TABLE 9 Exogenous Protease Cleavage Sites Protease
Cleavage Non-limiting SEQ ID Site Consensus Sequence Examples NO:
Bovine enterokinase DDDDK* DDDDK* 40 Tobacco Etch Virus E P.sup.5
P.sup.4YP.sup.2Q*(G/S), ENLYFQ*G 41 (TEV) where P.sup.2, P.sup.4
and P.sup.5 can be any ENLYFQ*S 42 amino acid ENIYTQ*G 43 ENIYTQ*S
44 ENIYLQ*G 45 ENIYLQ*S 46 ENVYFQ*G 47 ENVYSQ*S 48 ENVYSQ*G 49
ENVYSQ*S 50 Human Rhinovirus 3C P.sup.5P.sup.4LFQ*GP EALFQ*GP 51
where P.sup.4 is G, A, V, L, I, M, EVLFQ*GP 52 S or T and P.sup.5
can any amino ELLFQ*GP 53 acid, with D or E preferred. DALFQ*GP 54
DVLFQ*GP 55 DLLFQ*GP 56 SUMO/ULP-1 Tertiary structure
polypeptide-G* 57 Thrombin P.sup.3P.sup.2(R/K)*P.sup.1', GVR*G 58
where P.sup.3 is any amino acid and SAR*G 59 P.sup.2 or P.sup.1 is
G with the other SLR*G 60 position being any amino acid DGR*I 61
QGK*I 62 Thrombin P.sup.4P.sup.3P(R/K)*P.sup.1'P.sup.2' LVPR*GS 63
where P.sup.1' and P.sup.2' can be any LVPK*GS 64 amino acid except
for acidic FIPR*TF 65 amino acids like D or E; and VLPR*SF 66
P.sup.3 and P.sup.4 are hydrophobic IVPR*SF 67 amino acids like F,
L, I, Y, IVPR*GY 68 W, V, M, P, C or A VVPR*GV 69 VLPR*LI 70
VMPR*SL 71 MFPR*SL 72 Coagulation Factor I(E/D)GR* IDGR* 73 Xa
IEGR* 74 An asterisks (*) indicates the peptide bond that is
cleaved by the indicated protease.
[0694] As mentioned above, a Clostridial toxin is converted from a
single polypeptide form into a di-chain molecule by proteolytic
cleavage. While the naturally-occurring protease is currently not
known, cleavage occurs within the di-chain loop region between the
two cysteine residues that form the disulfide bridge (see Table 8).
Replacement of an endogenous protease cleavage site with an
exogenous protease cleavage site will enable cleavage of a modified
Clostridial toxin disclosed in the present specification when
expressed in an organism that does not produce the
naturally-occurring protease used to cleave the di-chain loop
region of a toxin. Similarly, an addition of an exogenous protease
cleavage site in the di-chain loop region will also enable cleavage
of a modified Clostridial toxin disclosed in the present
specification when expressed in an organism that does not produce
the naturally-occurring protease used to cleave the di-chain loop
region of a toxin.
[0695] It is envisioned that an exogenous protease cleavage site of
any and all lengths can be useful in aspects of the present
invention with the proviso that the exogenous protease cleavage
site is capable of being cleaved by its respective protease. Thus,
in aspects of this embodiment, an exogenous protease cleavage site
can be, e.g., at least 6 amino acids in length, at least 7 amino
acids in length, at least 8 amino acids in length, at least 9 amino
acids in length, at least 10 amino acids in length, at least 15
amino acids in length, at least 20 amino acids in length, at least
25 amino acids in length, at least 30 amino acids in length, at
least 40 amino acids in length, at least 50 amino acids in length
or at least 60 amino acids in length. In other aspects of this
embodiment, an exogenous protease cleavage site can be, e.g., at
most 6 amino acids in length, at most 7 amino acids in length, at
most 8 amino acids in length, at most 9 amino acids in length, at
most 10 amino acids in length, at most 15 amino acids in length, at
most 20 amino acids in length, at most 25 amino acids in length, at
most 30 amino acids in length, at most 40 amino acids in length, at
most 50 amino acids in length or at most 60 amino acids in
length.
[0696] In aspects of this embodiment, a di-chain loop region can be
modified to substitute a naturally-occurring protease cleavage site
for an exogenous protease cleavage site. In this type of
modification, the naturally-occurring protease cleavage site is
made inoperable and thus can not be cleaved by its protease. Only
the exogenous protease cleavage site can be cleaved by its
corresponding exogenous protease. In this type of modification, the
exogenous protease site is operably-linked in-frame to a modified
Clostridial toxin as a fusion protein and the site can be cleaved
by its respective exogenous protease. As a non-limiting example, a
single-chain modified BoNT/A comprising an exogenous protease
cleavage site in the di-chain loop region can be cleaved by its
respective exogenous protease to produce the di-chain form of the
toxin.
[0697] In other aspects of this embodiment, a di-chain loop region
can be modified to include an exogenous protease cleavage site in
addition to the naturally-occurring protease cleavage site. In this
type of modification, both cleavage sites are operably-linked
in-frame to a modified Clostridial toxin as a fusion protein and
both sites can be cleaved by their respective proteases. As a
non-limiting example, a single-chain modified BoNT/A that comprises
a di-chain loop containing both the naturally-occurring BoNT/A
di-chain loop protease cleavage site and an exogenous protease
cleavage site can be cleaved by either the naturally occurring
di-chain loop protease or by the appropriate exogenous protease to
produce the di-chain form of the toxin.
[0698] A naturally-occurring protease cleavage site can be made
inoperable by altering at least the two amino acids flanking the
peptide bond cleaved by the naturally-occurring di-chain loop
protease. More extensive alterations can be made, with the proviso
that the two cysteine residues of the di-chain loop region remain
intact and can still form the disulfide bridge. Non-limiting
examples of an amino acid alteration include deletion of an amino
acid or replacement of the original amino acid with a different
amino acid. Thus, in one embodiment, a naturally-occurring protease
cleavage site is made inoperable by altering the two amino acids
flanking the peptide bond cleaved by a naturally-occurring
protease. In other aspects of this embodiment, a
naturally-occurring protease cleavage site is made inoperable by
altering, e.g., at least three amino acids including the two amino
acids flanking the peptide bond cleaved by a naturally-occurring
protease; at least four amino acids including the two amino acids
flanking the peptide bond cleaved by a naturally-occurring
protease; at least five amino acids including the two amino acids
flanking the peptide bond cleaved by a naturally-occurring
protease; at least six amino acids including the two amino acids
flanking the peptide bond cleaved by a naturally-occurring
protease; at least seven amino acids including the two amino acids
flanking the peptide bond cleaved by a naturally-occurring
protease; at least eight amino acids including the two amino acids
flanking the peptide bond cleaved by a naturally-occurring
protease; at least nine amino acids including the two amino acids
flanking the peptide bond cleaved by a naturally-occurring
protease; at least ten amino acids including the two amino acids
flanking the peptide bond cleaved by a naturally-occurring
protease; at least 15 amino acids including the two amino acids
flanking the peptide bond cleaved by a naturally-occurring
protease; or at least 20 amino acids including the two amino acids
flanking the peptide bond cleaved by a naturally-occurring
protease.
[0699] In still other aspects of this embodiment, a
naturally-occurring di-chain protease cleavage site is made
inoperable by altering, e.g., at most three amino acids including
the two amino acids flanking the peptide bond cleaved by a
naturally-occurring protease; at most four amino acids including
the two amino acids flanking the peptide bond cleaved by a
naturally-occurring protease; at most five amino acids including
the two amino acids flanking the peptide bond cleaved by a
naturally-occurring protease; at most six amino acids including the
two amino acids flanking the peptide bond cleaved by a
naturally-occurring protease; at most seven amino acids including
the two amino acids flanking the peptide bond cleaved by a
naturally-occurring protease; at most eight amino acids including
the two amino acids flanking the peptide bond cleaved by a
naturally-occurring protease; at most nine amino acids including
the two amino acids flanking the peptide bond cleaved by a
naturally-occurring protease; at most ten amino acids including the
two amino acids flanking the peptide bond cleaved by a
naturally-occurring protease; at most 15 amino acids including the
two amino acids flanking the peptide bond cleaved by a
naturally-occurring protease; or at most 20 amino acids including
the two amino acids flanking the peptide bond cleaved by a
naturally-occurring protease.
[0700] In an embodiment, an exogenous protease cleavage site is
located within the di-chain loop of a modified Clostridial toxin.
In aspects of this embodiment, a modified Clostridial toxin
comprises an exogenous protease cleavage site comprises, e.g., a
bovine enterokinase protease cleavage site, a Tobacco Etch Virus
protease cleavage site, a Human Rhinovirus 3C protease cleavage
site, a SUMO/ULP-1 protease cleavage site, a Thrombin protease
cleavage site or a Factor Xa protease cleavage site. In other
aspects of this embodiment, an exogenous protease cleavage site is
located within the di-chain loop of, e.g., a modified BoNT/A, a
modified BoNT/B, a modified BoNT/C1, a modified BoNT/D, a modified
BoNT/E, a modified BoNT/F, a modified BoNT/G or a modified
TeNT.
[0701] In an aspect of this embodiment, an exogenous protease
cleavage site can be, e.g., a bovine enterokinase cleavage site is
located within the di-chain loop of a modified Clostridial toxin.
In other aspects of the embodiment, an exogenous protease cleavage
site can be, e.g., a bovine enterokinase protease cleavage site
located within the di-chain loop of a modified Clostridial toxin
comprises SEQ ID NO: 40. Is still other aspects of this embodiment,
a bovine enterokinase protease cleavage site is located within the
di-chain loop of, e.g., a modified BoNT/A, a modified BoNT/B, a
modified BoNT/C1, a modified BoNT/D, a modified BoNT/E, a modified
BoNT/F, a modified BoNT/G or a modified TeNT.
[0702] In another aspect of this embodiment, an exogenous protease
cleavage site can be, e.g., a Tobacco Etch Virus protease cleavage
site is located within the di-chain loop of a modified Clostridial
toxin. In other aspects of the embodiment, an exogenous protease
cleavage site can be, e.g., a Tobacco Etch Virus protease cleavage
site located within the di-chain loop of a modified Clostridial
toxin comprises SEQ ID NO: 41, SEQ ID NO: 42, SEQ ID NO: 43, SEQ ID
NO: 44, SEQ ID NO: 45, SEQ ID NO: 46, SEQ ID NO: 47, SEQ ID NO: 48,
SEQ ID NO: 49 or SEQ ID NO: 50. Is still other aspects of this
embodiment, a Tobacco Etch Virus protease cleavage site is located
within the di-chain loop of, e.g., a modified BoNT/A, a modified
BoNT/B, a modified BoNT/C1, a modified BoNT/D, a modified BoNT/E, a
modified BoNT/F, a modified BoNT/G or a modified TeNT.
[0703] In still another aspect of this embodiment, an exogenous
protease cleavage site can be, e.g., a Human Rhinovirus 3C protease
cleavage site is located within the di-chain loop of a modified
Clostridial toxin. In other aspects of the embodiment, an exogenous
protease cleavage site can be, e.g., a Human Rhinovirus 3C protease
cleavage site located within the di-chain loop of a modified
Clostridial toxin comprises SEQ ID NO: 51, SEQ ID NO: 52, SEQ ID
NO: 53, SEQ ID NO: 54, SEQ ID NO: 55 or SEQ ID NO: 56. Is still
other aspects of this embodiment, a Human Rhinovirus 3C protease
cleavage site is located within the di-chain loop of, e.g., a
modified BoNT/A, a modified BoNT/B, a modified BoNT/C1, a modified
BoNT/D, a modified BoNT/E, a modified BoNT/F, a modified BoNT/G or
a modified TeNT.
[0704] In yet another aspect of this embodiment, an exogenous
protease cleavage site can be, e.g., a SUMO/ULP-1 protease cleavage
site is located within the di-chain loop of a modified Clostridial
toxin. In other aspects of the embodiment, an exogenous protease
cleavage site can be, e.g., a SUMO/ULP-1 protease cleavage site
located within the di-chain loop of a modified Clostridial toxin
comprises SEQ ID NO: 57. Is still other aspects of this embodiment,
a SUMO/ULP-1 protease cleavage site is located within the di-chain
loop of, e.g., a modified BoNT/A, a modified BoNT/B, a modified
BoNT/C1, a modified BoNT/D, a modified BoNT/E, a modified BoNT/F, a
modified BoNT/G or a modified TeNT.
[0705] In a further aspect of this embodiment, an exogenous
protease cleavage site can be, e.g., a Thrombin protease cleavage
site is located within the di-chain loop of a modified Clostridial
toxin. In other aspects of the embodiment, an exogenous protease
cleavage site can be, e.g., a Thrombin protease cleavage site
located within the di-chain loop of a modified Clostridial toxin
comprises SEQ ID NO: 58, SEQ ID NO: 59, SEQ ID NO: 60, SEQ ID NO:
61, SEQ ID NO: 62, SEQ ID NO: 63, SEQ ID NO: 64, SEQ ID NO: 65, SEQ
ID NO: 66, SEQ ID NO: 67, SEQ ID NO: 68, SEQ ID NO: 69, SEQ ID NO:
70, SEQ ID NO: 71 or SEQ ID NO: 72. Is still other aspects of this
embodiment, a Thrombin protease cleavage site is located within the
di-chain loop of, e.g., a modified BoNT/A, a modified BoNT/B, a
modified BoNT/C1, a modified BoNT/D, a modified BoNT/E, a modified
BoNT/F, a modified BoNT/G or a modified TeNT.
[0706] In another aspect of this embodiment, an exogenous protease
cleavage site can be, e.g., a Coagulation Factor Xa protease
cleavage site is located within the di-chain loop of a modified
Clostridial toxin. In other aspects of the embodiment, an exogenous
protease cleavage site can be, e.g., a Coagulation Factor Xa
protease cleavage site located within the di-chain loop of a
modified Clostridial toxin comprises SEQ ID NO: 73 or SEQ ID NO:
74. Is still other aspects of this embodiment, a Coagulation Factor
Xa protease cleavage site is located within the di-chain loop of,
e.g., a modified BoNT/A, a modified BoNT/B, a modified BoNT/C1, a
modified BoNT/D, a modified BoNT/E, a modified BoNT/F, a modified
BoNT/G or a modified TeNT.
[0707] In another embodiment, an exogenous protease site comprises
a Clostridial toxin substrate cleavage site. As used herein, the
term "Clostridial toxin substrate cleavage site" means a scissile
bond together with adjacent or non-adjacent recognition elements,
or both, sufficient for detectable proteolysis at the scissile bond
by a Clostridial toxin under conditions suitable for Clostridial
toxin protease activity. By definition, a Clostridial toxin
substrate cleavage site is susceptible to cleavage by at least one
Clostridial toxin under conditions suitable for Clostridial toxin
protease activity. Non-limiting examples of Clostridial toxin
substrate cleavage site are disclosed in, e.g., Steward, L. E. et
al., Self-Activating Clostridial Toxins, U.S. Patent Application
60/718,616 (Sep., 19, 2005).
[0708] It is understood that a modified Clostridial toxin disclosed
in the present specification can optionally include one or more
additional components. As a non-limiting example of an optional
component, a modified Clostridial toxin can further comprise a
flexible region comprising a flexible spacer. Non-limiting examples
of a flexible spacer include, e.g., a G-spacer GGGGS (SEQ ID NO:
75) or an A-spacer EAAAK (SEQ ID NO: 76). A flexible region
comprising flexible spacers can be used to adjust the length of a
polypeptide region in order to optimize a characteristic, attribute
or property of a polypeptide. Such a flexible region is
operably-linked in-frame to the modified Clostridial toxin as a
fusion protein. As a non-limiting example, a polypeptide region
comprising one or more flexible spacers in tandem can be use to
better expose a protease cleavage site thereby facilitating
cleavage of that site by a protease. As another non-limiting
example, a polypeptide region comprising one or more flexible
spacers in tandem can be use to better present an enhanced
targeting domain, thereby facilitating the binding of that enhanced
targeting domain to its receptor.
[0709] Thus, in an embodiment, a modified Clostridial toxin
disclosed in the present specification can further comprise a
flexible region comprising a flexible spacer. In another
embodiment, a modified Clostridial toxin disclosed in the present
specification can further comprise flexible region comprising a
plurality of flexible spacers in tandem. In aspects of this
embodiment, a flexible region can comprise in tandem, e.g., at
least 1 G-spacer, at least 2 G-spacers, at least 3 G-spacers, at
least 4 G-spacers or at least 5 G-spacers. In other aspects of this
embodiment, a flexible region can comprise in tandem, e.g., at most
1 G-spacer, at most 2 G-spacers, at most 3 G-spacers, at most 4
G-spacers or at most 5 G-spacers. In still other aspects of this
embodiment, a flexible region can comprise in tandem, e.g., at
least 1 A-spacer, at least 2 A-spacers, at least 3 A-spacers, at
least 4 A-spacers or at least 5 A-spacers. In still other aspects
of this embodiment, a flexible region can comprise in tandem, e.g.,
at most 1 A-spacer, at most 2 A-spacers, at most 3 A-spacers, at
most 4 A-spacers or at most 5 A-spacers. In another aspect of this
embodiment, a modified Clostridial toxin can comprise a flexible
region comprising one or more copies of the same flexible spacers,
one or more copies of different flexible-spacer regions, or any
combination thereof.
[0710] In aspects of this embodiment, a modified Clostridial toxin
comprising a flexible spacer can be, e.g., a modified BoNT/A, a
modified BoNT/B, a modified BoNT/C1, a modified BoNT/D, a modified
BoNT/E, a modified BoNT/F, a modified BoNT/G or a modified
TeNT.
[0711] It is envisioned that a modified Clostridial toxin disclosed
in the present specification can comprise a flexible spacer in any
and all locations with the proviso that modified Clostridial toxin
is capable of performing the intoxication process. In aspects of
this embodiment, a flexible spacer is positioned between, e.g., an
enzymatic domain and a translocation domain, an enzymatic domain
and an enhanced targeting domain, an enzymatic domain and a
protease cleavage site. In other aspects of this embodiment, a
G-spacer is positioned between, e.g., an enzymatic domain and a
translocation domain, an enzymatic domain and an enhanced targeting
domain, an enzymatic domain and a protease cleavage site. In other
aspects of this embodiment, a A-spacer is positioned between, e.g.,
an enzymatic domain and a translocation domain, an enzymatic domain
and an enhanced targeting domain, an enzymatic domain and a
protease cleavage site.
[0712] In other aspects of this embodiment, a flexible spacer is
positioned between, e.g., an enhanced targeting domain and a
translocation domain, an enhanced targeting domain and an enzymatic
domain, an enhanced targeting domain and a protease cleavage site.
In other aspects of this embodiment, a G-spacer is positioned
between, e.g., an enhanced targeting domain and a translocation
domain, an enhanced targeting domain and an enzymatic domain, an
enhanced targeting domain and a protease cleavage site. In other
aspects of this embodiment, a A-spacer is positioned between, e.g.,
an enhanced targeting domain and a translocation domain, an
enhanced targeting domain and an enzymatic domain, an enhanced
targeting domain and a protease cleavage site.
[0713] In yet other aspects of this embodiment, a flexible spacer
is positioned between, e.g., a translocation domain and an
enzymatic domain, an translocation domain and an enhanced targeting
domain, an translocation domain and a protease cleavage site. In
other aspects of this embodiment, a G-spacer is positioned between,
e.g., a translocation domain and an enzymatic domain, an
translocation domain and an enhanced targeting domain, an
translocation domain and a protease cleavage site. In other aspects
of this embodiment, a A-spacer is positioned between, e.g., a
translocation domain and an enzymatic domain, an translocation
domain and an enhanced targeting domain, a translocation domain and
a protease cleavage site.
[0714] As another non-limiting example of an optional component, a
modified Clostridial toxin can further comprise an epitope-binding
region. An epitope-binding region can be used in a wide variety of
procedures involving, e.g., protein purification and protein
visualization. Such an epitope-binding region is operably-linked
in-frame to a modified Clostridial toxin as a fusion protein.
Non-limiting examples of an epitope-binding region include, e.g.,
FLAG, Express.TM. (SEQ ID NO: 77), human Influenza virus
hemagluttinin (HA) (SEQ ID NO: 78), human p62.sup.c-Myc protein
(c-MYC) (SEQ ID NO: 79), Vesicular Stomatitis Virus Glycoprotein
(VSV-G) (SEQ ID NO: 80), Substance P (SEQ ID NO: 81),
glycoprotein-D precursor of Herpes simplex virus (HSV) (SEQ ID NO:
82), V5 (SEQ ID NO: 83), AU1 (SEQ ID NO: 84) and AU5 (SEQ ID NO:
85); affinity-binding, such as. e.g., polyhistidine (HIS) (SEQ ID
NO: 86), streptavidin binding peptide (strep), and biotin or a
biotinylation sequence; peptide-binding regions, such as. e.g., the
glutathione binding domain of glutathione-S-transferase, the
calmodulin binding domain of the calmodulin binding protein, and
the maltose binding domain of the maltose binding protein.
Non-limiting examples of specific protocols for selecting, making
and using an appropriate binding peptide are described in, e.g.,
Epitope Tagging, pp. 17.90-17.93 (Sambrook and Russell, eds.,
Molecular Cloning A Laboratory Manual, Vol. 3, 3.sup.rd ed. 2001);
Antibodies: A Laboratory Manual (Edward Harlow & David Lane,
eds., Cold Spring Harbor Laboratory Press, 2.sup.nd ed. 1998); and
Using Antibodies: A Laboratory Manual: Portable Protocol No. I
(Edward Harlow & David Lane, Cold Spring Harbor Laboratory
Press, 1998). In addition, non-limiting examples of binding
peptides as well as well-characterized reagents, conditions and
protocols are readily available from commercial vendors that
include, without limitation, BD Biosciences-Clontech, Palo Alto,
Calif.; BD Biosciences Pharmingen, San Diego, Calif.; Invitrogen,
Inc, Carlsbad, Calif.; QIAGEN, Inc., Valencia, Calif.; and
Stratagene, La Jolla, Calif. These protocols are routine procedures
well within the scope of one skilled in the art and from the
teaching herein.
[0715] Thus, in an embodiment, a modified Clostridial toxin
disclosed in the present specification can further comprise an
epitope-binding region. In another embodiment, a modified
Clostridial toxin disclosed in the present specification can
further comprises a plurality of epitope-binding regions. In
aspects of this embodiment, a modified Clostridial toxin can
comprise, e.g., at least 1 epitope-binding region, at least 2
epitope-binding regions, at least 3 epitope-binding regions, at
least 4 epitope-binding regions or at least 5 epitope-binding
regions. In other aspects of this embodiment, a modified
Clostridial toxin can comprise, e.g., at most 1 epitope-binding
region, at most 2 epitope-binding regions, at most 3
epitope-binding regions, at most 4 epitope-binding regions or at
most 5 epitope-binding regions. In another aspect of this
embodiment, a modified Clostridial toxin can comprise one or more
copies of the same epitope-binding region, one or more copies of
different epitope-binding regions, or any combination thereof.
[0716] The location of an epitope-binding region can be in various
positions, including, without limitation, at the amino terminus of
a modified Clostridial toxin, within a modified Clostridial toxin,
or at the carboxyl terminus of a modified Clostridial toxin. Thus,
in an embodiment, an epitope-binding region is located at the
amino-terminus of a modified Clostridial toxin. In such a location,
a start methionine should be placed in front of the epitope-binding
region. In addition, it is known in the art that when adding a
polypeptide that is operationally-linked to the amino terminus of
another polypeptide comprising the start methionine that the
original methionine residue can be deleted. This is due to the fact
that the added polypeptide will contain a new start methionine and
that the original start methionine may reduce optimal expression of
the fusion protein. In aspects of this embodiment, an
epitope-binding region located at the amino-terminus of a modified
Clostridial toxin disclosed in the present specification can be,
e.g., a FLAG, Express.TM. epitope-binding region, a human Influenza
virus hemagluttinin (HA) epitope-binding region, a human
p62.sup.c-Myc protein (c-MYC) epitope-binding region, a Vesicular
Stomatitis Virus Glycoprotein (VSV-G) epitope-binding region, a
Substance P epitope-binding region, a glycoprotein-D precursor of
Herpes simplex virus (HSV) epitope-binding region, a V5
epitope-binding region, a AU1 epitope-binding region, a AU5
epitope-binding region, a polyhistidine epitope-binding region, a
streptavidin binding peptide epitope-binding region, a biotin
epitope-binding region, a biotinylation epitope-binding region, a
glutathione binding domain of glutathione-S-transferase, a
calmodulin binding domain of the calmodulin binding protein or a
maltose binding domain of the maltose binding protein.
[0717] In another embodiment, an epitope-binding region is located
at the carboxyl-terminus of a modified Clostridial toxin. In
aspects of this embodiment, an epitope-binding region located at
the carboxyl-terminus of a modified Clostridial toxin disclosed in
the present specification can be, e.g., a FLAG, Express.TM.
epitope-binding region, a human Influenza virus hemagluttinin (HA)
epitope-binding region, a human p62.sup.c-Myc protein (c-MYC)
epitope-binding region, a Vesicular Stomatitis Virus Glycoprotein
(VSV-G) epitope-binding region, a Substance P epitope-binding
region, a glycoprotein-D precursor of Herpes simplex virus (HSV)
epitope-binding region, a V5 epitope-binding region, a AU1
epitope-binding region, a AU5 epitope-binding region, a
polyhistidine epitope-binding region, a streptavidin binding
peptide epitope-binding region, a biotin epitope-binding region, a
biotinylation epitope-binding region, a glutathione binding domain
of glutathione-S-transferase, a calmodulin binding domain of the
calmodulin binding protein or a maltose binding domain of the
maltose binding protein.
[0718] It is envisioned that a modified Clostridial toxin disclosed
in the present specification can comprise an enhance targeting
domain in any and all locations with the proviso that modified
Clostridial toxin is capable of performing the intoxication
process. Non-limiting examples include, locating an enhance
targeting domain at the amino terminus of a modified Clostridial
toxin (see FIG. 4); locating an enhance targeting domain between a
Clostridial toxin enzymatic domain and a Clostridial toxin
translocation domain of a modified Clostridial toxin (see FIG. 5);
and locating an enhance targeting domain at the carboxyl terminus
of a modified Clostridial toxin (see FIG. 6). The enzymatic domain
of naturally-occurring Clostridial toxins contains the native start
methionine. Thus, in domain organizations where the enzymatic
domain is not in the amino-terminal location an amino acid sequence
comprising the start methionine should be placed in front of the
amino-terminal domain. Likewise, where the enhanced targeting
domain is in the amino-terminal position, an amino acid sequence
comprising a start methionine and a protease cleavage site may be
operably-linked in situations in which the enhanced targeting
domain requires a free amino terminus, see, e.g., Shengwen Li et
al., Degradable Clostridial Toxins, International Patent
Application Publication WO 2006/026780 (Mar. 9, 2006). In addition,
it is known in the art that when adding a polypeptide that is
operably-linked to the amino terminus of another polypeptide
comprising the start methionine that the original methionine
residue can be deleted.
[0719] Thus, in an embodiment, a modified Clostridial toxin can
comprise an amino to carboxyl single polypeptide linear order
comprising a Clostridial toxin enzymatic domain, a Clostridial
toxin translocation domain, a Clostridial toxin translocation
facilitating domain and an enhanced targeting domain. In an aspect
of this embodiment, a modified Clostridial toxin can comprise an
amino to carboxyl single polypeptide linear order comprising a
Clostridial toxin enzymatic domain, a protease cleavage site, a
Clostridial toxin translocation domain, a Clostridial toxin
translocation facilitating domain and an enhanced targeting domain.
In another aspect of this embodiment, a modified Clostridial toxin
can comprise an amino to carboxyl single polypeptide linear order
comprising a Clostridial toxin enzymatic domain, an endogenous
protease cleavage site, a Clostridial toxin translocation domain, a
Clostridial toxin translocation facilitating domain and an enhanced
targeting domain. In another aspect of this embodiment, a modified
Clostridial toxin can comprise an amino to carboxyl single
polypeptide linear order comprising a Clostridial toxin enzymatic
domain, an exogenous protease cleavage site, a Clostridial toxin
translocation domain, a Clostridial toxin translocation
facilitating domain and an enhanced targeting domain.
[0720] In another embodiment, a modified Clostridial toxin can
comprise an amino to carboxyl single polypeptide linear order
comprising a Clostridial toxin enzymatic domain, an enhanced
targeting domain, a Clostridial toxin translocation domain and a
Clostridial toxin translocation facilitating domain. In an aspect
of this embodiment, a modified Clostridial toxin can comprise an
amino to carboxyl single polypeptide linear order comprising a
Clostridial toxin enzymatic domain, a protease cleavage site, an
enhanced targeting domain, a Clostridial toxin translocation domain
and a Clostridial toxin translocation facilitating domain. In
another aspect of this embodiment, a modified Clostridial toxin can
comprise an amino to carboxyl single polypeptide linear order
comprising a Clostridial toxin enzymatic domain, an endogenous
protease cleavage site, an enhanced targeting domain, a Clostridial
toxin translocation domain and a Clostridial toxin translocation
facilitating domain. In another aspect of this embodiment, a
modified Clostridial toxin can comprise an amino to carboxyl single
polypeptide linear order comprising a Clostridial toxin enzymatic
domain, an exogenous protease cleavage site, an enhanced targeting
domain, a Clostridial toxin translocation domain and a Clostridial
toxin translocation facilitating domain.
[0721] In another embodiment, a modified Clostridial toxin can
comprise an amino to carboxyl single polypeptide linear order
comprising an enhanced targeting domain, a Clostridial toxin
translocation domain, a Clostridial toxin translocation
facilitating domain and a Clostridial toxin enzymatic domain. In an
aspect of this embodiment, a modified Clostridial toxin can
comprise an amino to carboxyl single polypeptide linear order
comprising an enhanced targeting domain, a Clostridial toxin
translocation domain, a Clostridial toxin translocation
facilitating domain, a protease cleavage site and a Clostridial
toxin enzymatic domain. In another aspect of this embodiment, a
modified Clostridial toxin can comprise an amino to carboxyl single
polypeptide linear order comprising an enhanced targeting domain, a
Clostridial toxin translocation domain, a Clostridial toxin
translocation facilitating domain, an endogenous protease cleavage
site and a Clostridial toxin enzymatic domain. In another aspect of
this embodiment, a modified Clostridial toxin can comprise an amino
to carboxyl single polypeptide linear order comprising an enhanced
targeting domain, a Clostridial toxin translocation domain, a
Clostridial toxin translocation facilitating domain, an exogenous
protease cleavage site and a Clostridial toxin enzymatic
domain.
[0722] In another embodiment, a modified Clostridial toxin can
comprise an amino to carboxyl single polypeptide linear order
comprising an enhanced targeting domain, a Clostridial toxin
enzymatic domain, a Clostridial toxin translocation domain and a
Clostridial toxin translocation facilitating domain. In an aspect
of this embodiment, a modified Clostridial toxin can comprise an
amino to carboxyl single polypeptide linear order comprising an
enhanced targeting domain, a Clostridial toxin enzymatic domain, a
protease cleavage site, a Clostridial toxin translocation domain
and a Clostridial toxin translocation facilitating domain. In
another aspect of this embodiment, a modified Clostridial toxin can
comprise an amino to carboxyl single polypeptide linear order
comprising an enhanced targeting domain, a Clostridial toxin
enzymatic domain, an endogenous protease cleavage site, a
Clostridial toxin translocation domain and a Clostridial toxin
translocation facilitating domain. In another aspect of this
embodiment, a modified Clostridial toxin can comprise an amino to
carboxyl single polypeptide linear order comprising an enhanced
targeting domain, a Clostridial toxin enzymatic domain, an
exogenous protease cleavage site, a Clostridial toxin translocation
domain and a Clostridial toxin translocation facilitating
domain.
[0723] In another embodiment, a modified Clostridial toxin can
comprise an amino to carboxyl single polypeptide linear order
comprising a Clostridial toxin translocation domain, a Clostridial
toxin translocation facilitating domain, a Clostridial toxin
enzymatic domain and an enhanced targeting domain. In an aspect of
this embodiment, a modified Clostridial toxin can comprise an amino
to carboxyl single polypeptide linear order comprising a
Clostridial toxin translocation domain, a Clostridial toxin
translocation facilitating domain, a protease cleavage site, a
Clostridial toxin enzymatic domain and an enhanced targeting
domain. In another aspect of this embodiment, a modified
Clostridial toxin can comprise an amino to carboxyl single
polypeptide linear order comprising a Clostridial toxin
translocation domain, a Clostridial toxin translocation
facilitating domain, an endogenous protease cleavage site, a
Clostridial toxin enzymatic domain and an enhanced targeting
domain. In another aspect of this embodiment, a modified
Clostridial toxin can comprise an amino to carboxyl single
polypeptide linear order comprising a Clostridial toxin
translocation domain, a Clostridial toxin translocation
facilitating domain, an exogenous protease cleavage site, a
Clostridial toxin enzymatic domain and an enhanced targeting
domain.
[0724] Aspects of the present invention provide, in part modified
Clostridial toxins. Non-limiting examples of Clostridial toxin
modifications disclosed in the present specification include, e.g.,
addition of an enhanced targeting domain, addition of a protease
cleavage site, rearrangement of the enzymatic, translocation and
binding domains and addition of a spacer region. It is understood
that all such modifications do not substantially affect the ability
of a modified Clostridial toxin to intoxicate a cell. As used
herein, the term "do not substantially affect" means a modified
Clostridial toxin can still execute the overall cellular mechanism
whereby a Clostridial toxin enters a neuron and inhibits
neurotransmitter release and encompasses the binding of a
Clostridial toxin to a low or high affinity receptor complex, the
internalization of the toxin/receptor complex, the translocation of
the Clostridial toxin light chain into the cytoplasm and the
enzymatic modification of a Clostridial toxin substrate. In aspects
of this embodiment, the modified Clostridial toxin is, e.g., at
least 10% as toxic as a naturally-occurring Clostridial toxin, at
least 20% as toxic as a naturally-occurring Clostridial toxin, at
least 30% as toxic as a naturally-occurring Clostridial toxin, at
least 40% as toxic as a naturally-occurring Clostridial toxin, at
least 50% as toxic as a naturally-occurring Clostridial toxin, at
least 60% as toxic as a naturally-occurring Clostridial toxin, at
least 70% as toxic as a naturally-occurring Clostridial toxin, at
least 80% as toxic as a naturally-occurring Clostridial toxin, at
least 90% as toxic as a naturally-occurring Clostridial toxin or at
least 95% as toxic as a naturally-occurring Clostridial toxin. In
aspects of this embodiment, the modified Clostridial toxin is,
e.g., at most 10% as toxic as a naturally-occurring Clostridial
toxin, at most 20% as toxic as a naturally-occurring Clostridial
toxin, at most 30% as toxic as a naturally-occurring Clostridial
toxin, at most 40% as toxic as a naturally-occurring Clostridial
toxin, at most 50% as toxic as a naturally-occurring Clostridial
toxin, at most 60% as toxic as a naturally-occurring Clostridial
toxin, at most 70% as toxic as a naturally-occurring Clostridial
toxin, at most 80% as toxic as a naturally-occurring Clostridial
toxin, at most 90% as toxic as a naturally-occurring Clostridial
toxin or at most 95% as toxic as a naturally-occurring Clostridial
toxin.
[0725] Another aspect of the present invention provides
polynucleotide molecules encoding modified Clostridial toxins
disclosed in the present specification. It is envisioned that any
and all modified Clostridial toxin disclosed in the present
specification can be encoded by a polynucleotide molecule.
[0726] Aspects of the present invention provide, in part
polynucleotide molecules. As used herein, the term "polynucleotide
molecule" is synonymous with "nucleic acid molecule" and means a
polymeric form of nucleotides, such as, e.g., ribonucleotides and
deoxyribonucleotides, of any length. It is envisioned that any and
all polynucleotide molecules that can encode a modified Clostridial
toxin disclosed in the present specification can be useful,
including, without limitation naturally-occurring and
non-naturally-occurring DNA molecules and naturally-occurring and
non-naturally-occurring RNA molecules. Non-limiting examples of
naturally-occurring and non-naturally-occurring DNA molecules
include single-stranded DNA molecules, double-stranded DNA
molecules, genomic DNA molecules, cDNA molecules, vector
constructs, such as, e.g., plasmid constructs, phagmid constructs,
bacteriophage constructs, retroviral constructs and artificial
chromosome constructs. Non-limiting examples of naturally-occurring
and non-naturally-occurring RNA molecules include single-stranded
RNA, double stranded RNA and mRNA.
[0727] Thus, in an embodiment, a polynucleotide molecule encodes a
modified Clostridial toxin disclosed in the present
specification.
[0728] In another embodiment, a polynucleotide molecule encodes, in
part, a modified Clostridial toxin comprising a Clostridial toxin
enzymatic domain disclosed in the present specification. In an
aspect of this embodiment, a polynucleotide molecule encoding a
modified Clostridial toxin enzymatic domain comprises a naturally
occurring Clostridial toxin enzymatic domain variant, such as,
e.g., a Clostridial toxin enzymatic domain isoform or a Clostridial
toxin enzymatic domain subtype. In another aspect of this
embodiment, a polynucleotide molecule encoding a Clostridial toxin
enzymatic domain comprises a non-naturally occurring Clostridial
toxin enzymatic domain variant, such as, e.g., a conservative
Clostridial toxin enzymatic domain variant, a non-conservative
Clostridial toxin enzymatic domain variant or an active Clostridial
toxin enzymatic domain fragment, or any combination thereof. In
other aspects of this embodiment, a polynucleotide molecule
encoding a Clostridial toxin enzymatic domain comprises a BoNT/A
enzymatic domain, a BoNT/B enzymatic domain, a BoNT/C1 enzymatic
domain, a BoNT/D enzymatic domain, a BoNT/E enzymatic domain, a
BoNT/F enzymatic domain, a BoNT/G enzymatic domain, a TeNT
enzymatic domain, or active fragment thereof.
[0729] In another embodiment, a polynucleotide molecule encodes, in
part, a modified Clostridial toxin comprising a Clostridial toxin
translocation domain disclosed in the present specification. In an
aspect of this embodiment, a polynucleotide molecule encoding a
modified Clostridial toxin translocation domain comprises a
naturally occurring Clostridial toxin translocation domain variant,
such as, e.g., a Clostridial toxin translocation domain isoform or
a Clostridial toxin translocation domain subtype. In another aspect
of this embodiment, a polynucleotide molecule encoding a
Clostridial toxin translocation domain comprises a non-naturally
occurring Clostridial toxin translocation domain variant, such as,
e.g., a conservative Clostridial toxin translocation domain
variant, a non-conservative Clostridial toxin translocation domain
variant or an active Clostridial toxin translocation domain
fragment, or any combination thereof. In other aspects of this
embodiment, a polynucleotide molecule encoding a Clostridial toxin
translocation domain comprises a BoNT/A translocation domain, a
BoNT/B translocation domain, a BoNT/C1 translocation domain, a
BoNT/D translocation domain, a BoNT/E translocation domain, a
BoNT/F translocation domain, a BoNT/G translocation domain, a TeNT
translocation domain, or active fragment thereof.
[0730] In another embodiment, a polynucleotide molecule encodes, in
part, a modified Clostridial toxin comprising a translocation
facilitating domain disclosed in the present specification. In an
aspect of this embodiment, a polynucleotide molecule encoding a
translocation facilitating domain comprises a naturally occurring
Clostridial toxin translocation facilitating domain variant, such
as, e.g., a Clostridial toxin translocation facilitating domain
isoform or a Clostridial toxin translocation facilitating domain
subtype. In another aspect of this embodiment, a polynucleotide
molecule encoding a translocation facilitating domain comprises a
non-naturally occurring Clostridial toxin translocation
facilitating domain variant, such as, e.g., a conservative
Clostridial toxin translocation facilitating domain variant, a
non-conservative Clostridial toxin translocation facilitating
domain variant or an active Clostridial toxin translocation
facilitating domain fragment, or any combination thereof. In other
aspects of this embodiment, a polynucleotide molecule encoding a
Clostridial toxin translocation facilitating domain comprises a
BoNT/A translocation facilitating domain, a BoNT/B translocation
facilitating domain, a BoNT/C1 translocation facilitating domain, a
BoNT/D translocation facilitating domain, a BoNT/E translocation
facilitating domain, a BoNT/F translocation facilitating domain, a
BoNT/G translocation facilitating domain, a TeNT translocation
facilitating domain, or active fragment thereof. In yet another
aspect of this embodiment, a polynucleotide molecule encoding a
translocation domain comprises a naturally occurring enveloped
virus fusogenic peptide domain variant, such as, e.g., an enveloped
virus fusogenic peptide domain isoform or an enveloped virus
fusogenic peptide domain subtype. In another aspect of this
embodiment, a polynucleotide molecule encoding a translocation
domain comprises a non-naturally occurring enveloped virus
fusogenic peptide domain variant, such as, e.g., a conservative
enveloped virus fusogenic peptide domain variant, a
non-conservative enveloped virus fusogenic peptide domain variant
or an active enveloped virus fusogenic peptide domain fragment, or
any combination thereof. In other aspects of this embodiment, a
polynucleotide molecule encoding an enveloped virus fusogenic
peptide domain comprisesan influenzavirus fusogenic peptide domain,
an alphavirus fusogenic peptide domain, a vesiculovirus fusogenic
peptide domain, a respirovirus fusogenic peptide domain, a
morbillivirus fusogenic peptide domain, an avulavirus fusogenic
peptide domain, a henipavirus fusogenic peptide domain, a
metapneumovirus fusogenic peptide domain, a foamy virus fusogenic
peptide domain, or active fragment thereof.
[0731] In another embodiment, a polynucleotide molecule encodes, in
part, a modified Clostridial toxin comprising an enhanced targeting
domain disclosed in the present specification. In an aspect of this
embodiment, a polynucleotide molecule encoding an enhanced
targeting domain comprises a modified Clostridial toxin targeting
domain with enhanced binding activity disclosed in the present
specification. In an aspect of this embodiment, a polynucleotide
molecule encoding a modified Clostridial toxin targeting domain
with enhanced binding activity comprises a non-naturally occurring
Clostridial toxin targeting domain variant with enhanced binding
activity, such as, e.g., a conservative Clostridial toxin targeting
domain variant with enhanced binding activity, a non-conservative
Clostridial toxin targeting domain variant with enhanced binding
activity or an active Clostridial toxin targeting domain fragment
with enhanced binding activity, or any combination thereof. In
other aspects of this embodiment, a polynucleotide molecule
encoding a Clostridial toxin targeting domain with enhanced binding
activity comprises a BoNT/A targeting domain with enhanced binding
activity, a BoNT/B targeting domain with enhanced binding activity,
a BoNT/C1 targeting domain with enhanced binding activity, a BoNT/D
targeting domain with enhanced binding activity, a BoNT/E targeting
domain with enhanced binding activity, a BoNT/F targeting domain
with enhanced binding activity, a BoNT/G targeting domain with
enhanced binding activity, a TeNT targeting domain with enhanced
binding activity, or active fragment thereof.
[0732] In an aspect of this embodiment, an enhanced targeting
domain comprises a Clostridial NAP disclosed in the present
specification. In an aspect of this embodiment, a polynucleotide
molecule encoding a Clostridial NAP comprises a naturally occurring
Clostridial NAP variant, such as, e.g., a Clostridial NAP isoform
or a Clostridial NAP subtype. In an aspect of this embodiment, a
polynucleotide molecule encoding a NAP comprises a non-naturally
occurring Clostridial NAP variant, such as, e.g., a conservative
Clostridial NAP variant, a non-conservative Clostridial NAP variant
or an active Clostridial NAP fragment, or any combination thereof.
In other aspects of this embodiment, a polynucleotide molecule
encoding a Clostridial NAP comprises a Clostridial botulinum
serotype A HA-33, a Clostridial botulinum serotype B HA-33, a
Clostridial botulinum serotype C1 HA-33, a Clostridial botulinum
serotype D HA-33, a Clostridial botulinum serotype A HA-17, a
Clostridial botulinum serotype B HA-17, a Clostridial botulinum
serotype C1 HA-17, a Clostridial botulinum serotype D HA-17, a
Clostridial botulinum serotype A NTNH, a Clostridial botulinum
serotype B NTNH, a Clostridial botulinum serotype C1 NTNH, a
Clostridial botulinum serotype D NTNH, a Clostridial botulinum
serotype E NTNH, a Clostridial botulinum serotype F NTNH and a
Clostridial botulinum serotype G NTNH.
[0733] In an aspect of this embodiment, an enhanced targeting
domain comprises a Clostridial NAP with enhanced binding activity
disclosed in the present specification. In an aspect of this
embodiment, a polynucleotide molecule encoding a NAP with enhanced
binding activity comprises a non-naturally occurring Clostridial
NAP variant with enhanced binding activity, such as, e.g., a
conservative Clostridial NAP variant with enhanced binding
activity, a non-conservative Clostridial NAP variant with enhanced
binding activity or an active Clostridial NAP fragment with
enhanced binding activity, or any combination thereof. In other
aspects of this embodiment, a polynucleotide molecule encoding a
Clostridial NAP comprises a Clostridial botulinum serotype A HA-33
with enhanced binding activity, a Clostridial botulinum serotype B
HA-33 with enhanced binding activity, a Clostridial botulinum
serotype C1 HA-33 with enhanced binding activity, a Clostridial
botulinum serotype D HA-33 with enhanced binding activity, a
Clostridial botulinum serotype A HA-17 with enhanced binding
activity, a Clostridial botulinum serotype B HA-17 with enhanced
binding activity, a Clostridial botulinum serotype C1 HA-17 with
enhanced binding activity, a Clostridial botulinum serotype D HA-17
with enhanced binding activity, a Clostridial botulinum serotype A
NTNH with enhanced binding activity, a Clostridial botulinum
serotype B NTNH with enhanced binding activity, a Clostridial
botulinum serotype C1 NTNH with enhanced binding activity, a
Clostridial botulinum serotype D NTNH with enhanced binding
activity, a Clostridial botulinum serotype E NTNH with enhanced
binding activity, a Clostridial botulinum serotype F NTNH with
enhanced binding activity and a Clostridial botulinum serotype G
NTNH with enhanced binding activity.
[0734] In an aspect of this embodiment, an enhanced targeting
domain comprises a FGF disclosed in the present specification. In
an aspect of this embodiment, a polynucleotide molecule encoding a
FGF comprises a naturally occurring FGF variant, such as, e.g., a
FGF isoform or a FGF subtype. In an aspect of this embodiment, a
polynucleotide molecule encoding a FGF comprises a non-naturally
occurring FGF variant, such as, e.g., a conservative FGF variant, a
non-conservative FGF variant or an active FGF fragment, or any
combination thereof. In other aspects of this embodiment, a
polynucleotide molecule encoding a FGF comprises a FGF-1, a FGF-2,
a FGF-4, a FGF-8, a FGF-9, a FGF-17 and a FGF-18.
[0735] In an aspect of this embodiment, an enhanced targeting
domain comprises a FGF with enhanced binding activity disclosed in
the present specification. In an aspect of this embodiment, a
polynucleotide molecule encoding a FGF with enhanced binding
activity comprises a non-naturally occurring FGF variant with
enhanced binding activity, such as, e.g., a conservative FGF
variant with enhanced binding activity, a non-conservative FGF
variant with enhanced binding activity or an active FGF fragment
with enhanced binding activity, or any combination thereof. In
other aspects of this embodiment, a polynucleotide molecule
encoding a FGF with enhanced binding activity comprises a FGF-1
with enhanced binding activity, a FGF-2 with enhanced binding
activity, a FGF-4 with enhanced binding activity, a FGF-8 with
enhanced binding activity, a FGF-9 with enhanced binding activity,
a FGF-17 with enhanced binding activity and a FGF-18 with enhanced
binding activity.
[0736] In another embodiment, a polynucleotide molecule encodes, in
part, a modified Clostridial toxin comprising a protease cleavage
site disclosed in the present specification. In an aspect of this
embodiment, a polynucleotide molecule encoding a protease cleavage
site comprises an endogenous Clostridial toxin protease site. In
aspects of this embodiment, a polynucleotide molecule encoding an
endogenous Clostridial toxin protease site can be, e.g., a BoNT/A
di-chain loop protease cleavage site, a BoNT/B di-chain loop
protease cleavage site, a BoNT/C1 di-chain loop protease cleavage
site, a BoNT/D di-chain loop protease cleavage site, a BoNT/E
di-chain loop protease cleavage site, a BoNT/F di-chain loop
protease cleavage site, a BoNT/G di-chain loop protease cleavage
site or a TeNT di-chain loop protease cleavage site. In another
aspect of this embodiment, a polynucleotide molecule encoding a
protease cleavage site comprises an exogenous Clostridial toxin
protease site. In aspects of this embodiment, a polynucleotide
molecule encoding an exogenous Clostridial toxin protease site can
be, e.g., a bovine enterokinase protease cleavage site, a Tobacco
Etch Virus protease cleavage site, a Human Rhinovirus 3C protease
cleavage site, a SUMO/ULP-1 protease cleavage site, a Thrombin
protease cleavage site, a Coagulation Factor Xa protease cleavage
site or a Clostridial toxin substrate cleavage site, such as, e.g.,
a BoNT/A substrate cleavage site, a BoNT/B substrate cleavage site,
a BoNT/C1 substrate cleavage site, a BoNT/D substrate cleavage
site, a BoNT/E substrate cleavage site, a BoNT/F substrate cleavage
site, a BoNT/G substrate cleavage site or a TeNT substrate cleavage
site.
[0737] In another embodiment, a polynucleotide molecule encodes, in
part, a modified Clostridial toxin comprising a flexible spacer
disclosed in the present specification. In an aspect of this
embodiment, a polynucleotide molecule encoding a flexible spacer
can comprise a G-spacer, a A-spacer or any combination thereof.
[0738] In another embodiment, a polynucleotide molecule encodes, in
part, a modified Clostridial toxin comprising an epitope-binding
region disclosed in the present specification. In an aspect of this
embodiment, a polynucleotide molecule encoding an epitope-binding
region can comprise a FLAG, Express.TM., a human Influenza virus
hemagluttinin (HA), a human p62.sup.c-Myc protein (c-MYC), a
Vesicular Stomatitis Virus Glycoprotein (VSV-G), a Substance P, a
glycoprotein-D precursor of Herpes simplex virus (HSV), a V5, a
AU1, a AU5 (SEQ ID NO: 85), a polyhistidine (HIS), or any
combination thereof.
[0739] Well-established molecular biology techniques that may be
necessary to make a polynucleotide molecule encoding a modified
Clostridial toxin disclosed in the present specification including,
but not limited to, procedures involving polymerase chain reaction
(PCR) amplification, restriction enzyme reactions, agarose gel
electrophoresis, nucleic acid ligation, bacterial transformation,
nucleic acid purification, nucleic acid sequencing and
recombination-based techniques are routine procedures well within
the scope of one skilled in the art and from the teaching herein.
Non-limiting examples of specific protocols necessary to make a
polynucleotide molecule encoding a modified Clostridial toxin are
described in e.g., MOLECULAR CLONING A LABORATORY MANUAL, supra,
(2001); and CURRENT PROTOCOLS IN MOLECULAR BIOLOGY (Frederick M.
Ausubel et al., eds. John Wiley & Sons, 2004). Additionally, a
variety of commercially available products useful for making a
polynucleotide molecule encoding a modified Clostridial toxin are
widely available. These protocols are routine procedures well
within the scope of one skilled in the art and from the teaching
herein.
[0740] Another aspect of the present invention provides a method of
producing a modified Clostridial toxin disclosed in the present
specification, such method comprising the step of expressing a
polynucleotide molecule encoding a modified Clostridial toxin in a
cell. Another aspect of the present invention provides a method of
producing a modified Clostridial toxin disclosed in the present
specification, such method comprising the steps of introducing an
expression construct comprising a polynucleotide molecule encoding
a modified Clostridial toxin into a cell and expressing the
expression construct in the cell.
[0741] The methods disclosed in the present specification include,
in part, a modified Clostridial toxin. It is envisioned that any
and all modified Clostridial toxins disclosed in the present
specification can be produced using the methods disclosed in the
present specification. It is also envisioned that any and all
polynucleotide molecules encoding a modified Clostridial toxins
disclosed in the present specification can be useful in producing a
modified Clostridial toxins disclosed in the present specification
using the methods disclosed in the present specification.
[0742] The methods disclosed in the present specification include,
in part, an expression construct. An expression construct comprises
a polynucleotide molecule disclosed in the present specification
operably-linked to an expression vector useful for expressing the
polynucleotide molecule in a cell or cell-free extract. A wide
variety of expression vectors can be employed for expressing a
polynucleotide molecule encoding a modified Clostridial toxin,
including, without limitation, a viral expression vector; a
prokaryotic expression vector; eukaryotic expression vectors, such
as, e.g., a yeast expression vector, an insect expression vector
and a mammalian expression vector; and a cell-free extract
expression vector. It is further understood that expression vectors
useful to practice aspects of these methods may include those which
express a modified Clostridial toxin under control of a
constitutive, tissue-specific, cell-specific or inducible promoter
element, enhancer element or both. Non-limiting examples of
expression vectors, along with well-established reagents and
conditions for making and using an expression construct from such
expression vectors are readily available from commercial vendors
that include, without limitation, BD Biosciences-Clontech, Palo
Alto, Calif.; BD Biosciences Pharmingen, San Diego, Calif.;
Invitrogen, Inc, Carlsbad, Calif.; EMD Biosciences-Novagen,
Madison, Wis.; QIAGEN, Inc., Valencia, Calif.; and Stratagene, La
Jolla, Calif. The selection, making and use of an appropriate
expression vector are routine procedures well within the scope of
one skilled in the art and from the teachings herein.
[0743] Thus, aspects of this embodiment include, without
limitation, a viral expression vector operably-linked to a
polynucleotide molecule encoding a modified Clostridial toxin; a
prokaryotic expression vector operably-linked to a polynucleotide
molecule encoding a modified Clostridial toxin; a yeast expression
vector operably-linked to a polynucleotide molecule encoding a
modified Clostridial toxin; an insect expression vector
operably-linked to a polynucleotide molecule encoding a modified
Clostridial toxin; and a mammalian expression vector
operably-linked to a polynucleotide molecule encoding a modified
Clostridial toxin. Other aspects of this embodiment include,
without limitation, expression constructs suitable for expressing a
modified Clostridial toxin disclosed in the present specification
using a cell-free extract comprising a cell-free extract expression
vector operably linked to a polynucleotide molecule encoding a
modified Clostridial toxin. Other aspects of this embodiment
include, without limitation, expression constructs comprising
polynucleotide molecules comprising any one of SEQ ID NO: 109
through SEQ ID NO: 132 and SEQ ID NO: 136 through SEQ ID NO: 159.
Other aspects of this embodiment include, without limitation,
expression constructs comprising polynucleotide molecules encoding
a modified Clostridial toxin comprising any one of SEQ ID NO: 85
through SEQ ID NO: 108.
[0744] The methods disclosed in the present specification include,
in part, a cell. It is envisioned that any and all cells can be
used. Thus, aspects of this embodiment include, without limitation,
prokaryotic cells including, without limitation, strains of
aerobic, microaerophilic, capnophilic, facultative, anaerobic,
gram-negative and gram-positive bacterial cells such as those
derived from, e.g., Escherichia coli, Bacillus subtilis, Bacillus
lichenifonnis, Bacteroides fragilis, Clostridia perfringens,
Clostridia difficile, Caulobacter crescentus, Lactococcus lactis,
Methylobacterium extorquens, Neisseria meningirulls, Neisseria
meningitidis, Pseudomonas fluorescens and Salmonella typhimurium;
and eukaryotic cells including, without limitation, yeast strains,
such as, e.g., those derived from Pichia pastoris, Pichia
methanolica, Pichia angusta, Schizosaccharomyces pombe,
Saccharomyces cerevisiae and Yarrowia lipolytica; insect cells and
cell lines derived from insects, such as, e.g., those derived from
Spodoptera frugiperda, Trichoplusia ni, Drosophila melanogaster and
Manduca sexta; and mammalian cells and cell lines derived from
mammalian cells, such as, e.g., those derived from mouse, rat,
hamster, porcine, bovine, equine, primate and human. Cell lines may
be obtained from the American Type Culture Collection, European
Collection of Cell Cultures and the German Collection of
Microorganisms and Cell Cultures. Non-limiting examples of specific
protocols for selecting, making and using an appropriate cell line
are described in e.g., INSECT CELL CULTURE ENGINEERING (Mattheus F.
A. Goosen et al. eds., Marcel Dekker, 1993); INSECT CELL CULTURES:
FUNDAMENTAL AND APPLIED ASPECTS (J. M. Vlak et al. eds., Kluwer
Academic Publishers, 1996); Maureen A. Harrison & Ian F. Rae,
GENERAL TECHNIQUES OF CELL CULTURE (Cambridge University Press,
1997); CELL AND TISSUE CULTURE: LABORATORY PROCEDURES (Alan Doyle
et al eds., John Wiley and Sons, 1998); R. Ian Freshney, CULTURE OF
ANIMAL CELLS: A MANUAL OF BASIC TECHNIQUE (Wiley-Liss, 4.sup.th ed.
2000); ANIMAL CELL CULTURE: A PRACTICAL APPROACH (John R. W.
Masters ed., Oxford University Press, 3.sup.rd ed. 2000); MOLECULAR
CLONING A LABORATORY MANUAL, supra, (2001); BASIC CELL CULTURE: A
PRACTICAL APPROACH (John M. Davis, Oxford Press, 2.sup.nd ed.
2002); and CURRENT PROTOCOLS IN MOLECULAR BIOLOGY, supra, (2004).
These protocols are routine procedures within the scope of one
skilled in the art and from the teaching herein.
[0745] The methods disclosed in the present specification include,
in part, introducing into a cell a polynucleotide molecule. A
polynucleotide molecule introduced into a cell can be transiently
or stably maintained by that cell. Stably-maintained polynucleotide
molecules may be extra-chromosomal and replicate autonomously, or
they may be integrated into the chromosomal material of the cell
and replicate non-autonomously. It is envisioned that any and all
methods for introducing a polynucleotide molecule disclosed in the
present specification into a cell can be used. Methods useful for
introducing a nucleic acid molecule into a cell include, without
limitation, chemical-mediated transfection such as, e.g., calcium
phosphate-mediated, diethyl-aminoethyl (DEAE) dextran-mediated,
lipid-mediated, polyethyleneimine (PEI)-mediated,
polylysine-mediated and polybrene-mediated; physical-mediated
transfection, such as, e.g., biolistic particle delivery,
microinjection, protoplast fusion and electroporation; and
viral-mediated transfection, such as, e.g., retroviral-mediated
transfection, see, e.g., Introducing Cloned Genes into Cultured
Mammalian Cells, pp. 16.1-16.62 (Sambrook & Russell, eds.,
Molecular Cloning A Laboratory Manual, Vol. 3, 3.sup.rd ed. 2001).
One skilled in the art understands that selection of a specific
method to introduce an expression construct into a cell will
depend, in part, on whether the cell will transiently contain an
expression construct or whether the cell will stably contain an
expression construct. These protocols are routine procedures within
the scope of one skilled in the art and from the teaching
herein.
[0746] In an aspect of this embodiment, a chemical-mediated method,
termed transfection, is used to introduce a polynucleotide molecule
encoding a modified Clostridial toxin into a cell. In
chemical-mediated methods of transfection the chemical reagent
forms a complex with the nucleic acid that facilitates its uptake
into the cells. Such chemical reagents include, without limitation,
calcium phosphate-mediated, see, e.g., Martin Jordan & Florian
Worm, Transfection of adherent and suspended cells by calcium
phosphate, 33(2) Methods 136-143 (2004); diethyl-aminoethyl (DEAE)
dextran-mediated, lipid-mediated, cationic polymer-mediated like
polyethyleneimine (PEI)-mediated and polylysine-mediated and
polybrene-mediated, see, e.g., Chun Zhang et al., Polyethylenimine
strategies for plasmid delivery to brain-derived cells, 33(2)
Methods 144-150 (2004). Such chemical-mediated delivery systems can
be prepared by standard methods and are commercially available,
see, e.g., CellPhect Transfection Kit (Amersham Biosciences,
Piscataway, N.J.); Mammalian Transfection Kit, Calcium phosphate
and DEAE Dextran, (Stratagene, Inc., La Jolla, Calif.);
Lipofectamine.TM. Transfection Reagent (Invitrogen, Inc., Carlsbad,
Calif.); ExGen 500 Transfection kit (Fermentas, Inc., Hanover,
Md.), and SuperFect and Effectene Transfection Kits (Qiagen, Inc.,
Valencia, Calif.).
[0747] In another aspect of this embodiment, a physical-mediated
method is used to introduce a polynucleotide molecule encoding a
modified Clostridial toxin into a cell. Physical techniques
include, without limitation, electroporation, biolistic and
microinjection. Biolistics and microinjection techniques perforate
the cell wall in order to introduce the nucleic acid molecule into
the cell, see, e.g., Jeike E. Biewenga et al., Plasmid-mediated
gene transfer in neurons using the biolistics technique, 71(1) J.
Neurosci. Methods. 67-75 (1997); and John O'Brien & Sarah C. R.
Lummis, Biolistic and diolistic transfection: using the gene gun to
deliver DNA and lipophilic dyes into mammalian cells, 33(2) Methods
121-125 (2004). Electroporation, also termed
electropermeabilization, uses brief, high-voltage, electrical
pulses to create transient pores in the membrane through which the
nucleic acid molecules enter and can be used effectively for stable
and transient transfections of all cell types, see, e.g., M. Golzio
et al., In vitro and in vivo electric field-mediated
permeabilization, gene transfer, and expression, 33(2) Methods
126-135 (2004); and Oliver Greschet al., New non-viral method for
gene transfer into primary cells, 33(2) Methods 151-163 (2004).
[0748] In another aspect of this embodiment, a viral-mediated
method, termed transduction, is used to introduce a polynucleotide
molecule encoding a modified Clostridial toxin into a cell. In
viral-mediated methods of transient transduction, the process by
which viral particles infect and replicate in a host cell has been
manipulated in order to use this mechanism to introduce a nucleic
acid molecule into the cell. Viral-mediated methods have been
developed from a wide variety of viruses including, without
limitation, retroviruses, adenoviruses, adeno-associated viruses,
herpes simplex viruses, picornaviruses, alphaviruses and
baculoviruses, see, e.g., Armin Blesch, Lentiviral and MLV based
retroviral vectors for ex vivo and in vivo gene transfer, 33(2)
Methods 164-172 (2004); and Maurizio Federico, From lentiviruses to
lentivirus vectors, 229 Methods Mol. Biol. 3-15 (2003); E. M.
Poeschla, Non-primate lentiviral vectors, 5(5) Curr. Opin. Mol.
Ther. 529-540 (2003); Karim Benihoud et al, Adenovirus vectors for
gene delivery, 10(5) Curr. Opin. Biotechnol. 440-447 (1999); H.
Bueler, Adeno-associated viral vectors for gene transfer and gene
therapy, 380(6) Biol. Chem. 613-622 (1999); Chooi M. Lai et al.,
Adenovirus and adeno-associated virus vectors, 21(12) DNA Cell
Biol. 895-913 (2002); Edward A. Burton et al., Gene delivery using
herpes simplex virus vectors, 21(12) DNA Cell Biol. 915-936 (2002);
Paola Grandi et al., Targeting HSV amplicon vectors, 33(2) Methods
179-186 (2004); Ilya Frolov et al., Alphavirus-based expression
vectors: strategies and applications, 93(21) Proc. Natl. Acad. Sci.
U.S.A. 11371-11377 (1996); Markus U. Ehrengruber, Alphaviral gene
transfer in neurobiology, 59(1) Brain Res. Bull. 13-22 (2002);
Thomas A. Kost & J. Patrick Condreay, Recombinant baculoviruses
as mammalian cell gene-delivery vectors, 20(4) Trends Biotechnol.
173-180 (2002); and A. Huser & C. Hofmann, Baculovirus vectors:
novel mammalian cell gene-delivery vehicles and their applications,
3(1) Am. J. Pharmacogenomics 53-63 (2003).
[0749] Adenoviruses, which are non-enveloped, double-stranded DNA
viruses, are often selected for mammalian cell transduction because
adenoviruses handle relatively large polynucleotide molecules of
about 36 kb, are produced at high titer, and can efficiently infect
a wide variety of both dividing and non-dividing cells, see, e.g.,
Wim T. J. M. C. Hermens et al., Transient gene transfer to neurons
and glia: analysis of adenoviral vector performance in the CNS and
PNS, 71(1) J. Neurosci. Methods 85-98 (1997); and Hiroyuki
Mizuguchi et al., Approaches for generating recombinant adenovirus
vectors, 52.beta.) Adv. Drug Deliv. Rev. 165-176 (2001).
Transduction using adenoviral-based system do not support prolonged
protein expression because the nucleic acid molecule is carried
from an episome in the cell nucleus, rather than being integrated
into the host cell chromosome. Adenoviral vector systems and
specific protocols for how to use such vectors are disclosed in,
e.g., ViraPower.TM. Adenoviral Expression System (Invitrogen, Inc.,
Carlsbad, Calif.) and ViraPower.TM. Adenoviral Expression System
Instruction Manual 25-0543 version A, Invitrogen, Inc., (Jul. 15,
2002); and AdEasy.TM. Adenoviral Vector System (Stratagene, Inc.,
La Jolla, Calif.) and AdEasy.TM. Adenoviral Vector System
Instruction Manual 064004f, Stratagene, Inc.
[0750] Nucleic acid molecule delivery can also use single-stranded
RNA retroviruses, such as, e.g., oncoretroviruses and lentiviruses.
Retroviral-mediated transduction often produce transduction
efficiencies close to 100%, can easily control the proviral copy
number by varying the multiplicity of infection (MOI), and can be
used to either transiently or stably transduce cells, see, e.g.,
Tiziana Tonini et al., Transient production of retroviral- and
lentiviral-based vectors for the transduction of Mammalian cells,
285 Methods Mol. Biol. 141-148 (2004); Armin Blesch, Lentiviral and
MLV based retroviral vectors for ex vivo and in vivo gene transfer,
33(2) Methods 164-172 (2004); Felix Recillas-Targa, Gene transfer
and expression in mammalian cell lines and transgenic animals, 267
Methods Mol. Biol. 417-433 (2004); and Roland Wolkowicz et al.,
Lentiviral vectors for the delivery of DNA into mammalian cells,
246 Methods Mol. Biol. 391-411 (2004). Retroviral particles consist
of an RNA genome packaged in a protein capsid, surrounded by a
lipid envelope. The retrovirus infects a host cell by injecting its
RNA into the cytoplasm along with the reverse transcriptase enzyme.
The RNA template is then reverse transcribed into a linear, double
stranded cDNA that replicates itself by integrating into the host
cell genome. Viral particles are spread both vertically (from
parent cell to daughter cells via the provirus) as well as
horizontally (from cell to cell via virions). This replication
strategy enables long-term persistent expression since the nucleic
acid molecules of interest are stably integrated into a chromosome
of the host cell, thereby enabling long-term expression of the
protein. For instance, animal studies have shown that lentiviral
vectors injected into a variety of tissues produced sustained
protein expression for more than 1 year, see, e.g., Luigi Naldini
et al., In vivo gene delivery and stable transduction of
non-dividing cells by a lentiviral vector, 272(5259) Science
263-267 (1996). The Oncoretroviruses-derived vector systems, such
as, e.g., Moloney murine leukemia virus (MoMLV), are widely used
and infect many different non-dividing cells. Lentiviruses can also
infect many different cell types, including dividing and
non-dividing cells and possess complex envelope proteins, which
allows for highly specific cellular targeting.
[0751] Retroviral vectors and specific protocols for how to use
such vectors are disclosed in, e.g., U.S. patent Nos. Manfred
Gossen & Hermann Bujard, Tight control of gene expression in
eukaryotic cells by tetracycline-responsive promoters, U.S. Pat.
No. 5,464,758 (Nov. 7, 1995) and Hermann Bujard & Manfred
Gossen, Methods for regulating gene expression, U.S. Pat. No.
5,814,618 (Sep. 29, 1998) David S. Hogness, Polynucleotides
encoding insect steroid hormone receptor polypeptides and cells
transformed with same, U.S. Pat. No. 5,514,578 (May 7, 1996) and
David S. Hogness, Polynucleotide encoding insect ecdysone receptor,
U.S. Pat. No. 6,245,531 (Jun. 12, 2001); Elisabetta Vegeto et al.,
Progesterone receptor having C. terminal hormone binding domain
truncations, U.S. Pat. No. 5,364,791 (Nov. 15, 1994), Elisabetta
Vegeto et al., Mutated steroid hormone receptors, methods for their
use and molecular switch for gene therapy, U.S. Pat. No. 5,874,534
(Feb. 23, 1999) and Elisabetta Vegeto et al., Mutated steroid
hormone receptors, methods for their use and molecular switch for
gene therapy, U.S. Pat. No. 5,935,934 (Aug. 10, 1999). Furthermore,
such viral delivery systems can be prepared by standard methods and
are commercially available, see, e.g., BD.TM. Tet-Off and Tet-On
Gene Expression Systems (BD Biosciences-Clonetech, Palo Alto,
Calif.) and BD.TM. Tet-Off and Tet-On Gene Expression Systems User
Manual, PT3001-1, BD Biosciences Clonetech, (Mar. 14, 2003),
GeneSwitch.TM. System (Invitrogen, Inc., Carlsbad, Calif.) and
GeneSwitch.TM. System A Mifepristone-Regulated Expression System
for Mammalian Cells version D, 25-0313, Invitrogen, Inc., (Nov. 4,
2002); ViraPower.TM. Lentiviral Expression System (Invitrogen,
Inc., Carlsbad, Calif.) and ViraPower.TM. Lentiviral Expression
System Instruction Manual 25-0501 version E, Invitrogen, Inc.,
(Dec. 8, 2003); and Complete Control.RTM. Retroviral Inducible
Mammalian Expression System (Stratagene, La Jolla, Calif.) and
Complete Control.RTM. Retroviral Inducible Mammalian Expression
System Instruction Manual, 064005e.
[0752] The methods disclosed in the present specification include,
in part, expressing a modified Clostridial toxin from a
polynucleotide molecule. It is envisioned that any of a variety of
expression systems may be useful for expressing a modified
Clostridial toxin from a polynucleotide molecule disclosed in the
present specification, including, without limitation, cell-based
systems and cell-free expression systems. Cell-based systems
include, without limitation, viral expression systems, prokaryotic
expression systems, yeast expression systems, baculoviral
expression systems, insect expression systems and mammalian
expression systems. Cell-free systems include, without limitation,
wheat germ extracts, rabbit reticulocyte extracts and E. coli
extracts and generally are equivalent to the method disclosed
herein. Expression of a polynucleotide molecule using an expression
system can include any of a variety of characteristics including,
without limitation, inducible expression, non-inducible expression,
constitutive expression, viral-mediated expression,
stably-integrated expression, and transient expression. Expression
systems that include well-characterized vectors, reagents,
conditions and cells are well-established and are readily available
from commercial vendors that include, without limitation, Ambion,
Inc. Austin, Tex.; BD Biosciences-Clontech, Palo Alto, Calif.; BD
Biosciences Pharmingen, San Diego, Calif.; Invitrogen, Inc,
Carlsbad, Calif.; QIAGEN, Inc., Valencia, Calif.; Roche Applied
Science, Indianapolis, Ind.; and Stratagene, La Jolla, Calif.
Non-limiting examples on the selection and use of appropriate
heterologous expression systems are described in e.g., PROTEIN
EXPRESSION. A PRACTICAL APPROACH (S. J. Higgins and B. David Hames
eds., Oxford University Press, 1999); Joseph M. Fernandez &
James P. Hoeffler, GENE EXPRESSION SYSTEMS. USING NATURE FOR THE
ART OF EXPRESSION (Academic Press, 1999); and Meena Rai &
Harish Padh, Expression Systems for Production of Heterologous
Proteins, 80(9) CURRENT SCIENCE 1121-1128, (2001). These protocols
are routine procedures well within the scope of one skilled in the
art and from the teaching herein.
[0753] A variety of cell-based expression procedures are useful for
expressing a modified Clostridial toxin encoded by polynucleotide
molecule disclosed in the present specification. Examples included,
without limitation, viral expression systems, prokaryotic
expression systems, yeast expression systems, baculoviral
expression systems, insect expression systems and mammalian
expression systems. Viral expression systems include, without
limitation, the ViraPower.TM. Lentiviral (Invitrogen, Inc.,
Carlsbad, Calif.), the Adenoviral Expression Systems (Invitrogen,
Inc., Carlsbad, Calif.), the AdEasy.TM. XL Adenoviral Vector System
(Stratagene, La Jolla, Calif.) and the ViraPort.RTM. Retroviral
Gene Expression System (Stratagene, La Jolla, Calif.). Non-limiting
examples of prokaryotic expression systems include the Champion.TM.
pET Expression System (EMD Biosciences-Novagen, Madison, Wis.), the
TriEx.TM. Bacterial Expression System (EMD Biosciences-Novagen,
Madison, Wis.), the QIAexpress.RTM. Expression System (QIAGEN,
Inc.), and the Affinity.RTM. Protein Expression and Purification
System (Stratagene, La Jolla, Calif.). Yeast expression systems
include, without limitation, the EasySelect.TM. Pichia Expression
Kit (Invitrogen, Inc., Carlsbad, Calif.), the YES-Echo.TM.
Expression Vector Kits (Invitrogen, Inc., Carlsbad, Calif.) and the
SpECTRA.TM. S. pombe Expression System (Invitrogen, Inc., Carlsbad,
Calif.). Non-limiting examples of baculoviral expression systems
include the BaculoDirect.TM. (Invitrogen, Inc., Carlsbad, Calif.),
the BactoBac.RTM. (Invitrogen, Inc., Carlsbad, Calif.), and the BD
BaculoGold.TM. (BD Biosciences-Pharmigen, San Diego, Calif.).
Insect expression systems include, without limitation, the
Drosophila Expression System (DES.RTM.) (Invitrogen, Inc.,
Carlsbad, Calif.), InsectSelect.TM. System (Invitrogen, Inc.,
Carlsbad, Calif.) and InsectDirect.TM. System (EMD
Biosciences-Novagen, Madison, Wis.). Non-limiting examples of
mammalian expression systems include the T-REx.TM.
(Tetracycline-Regulated Expression) System (Invitrogen, Inc.,
Carlsbad, Calif.), the Flp-In.TM. T-REx.TM. System (Invitrogen,
Inc., Carlsbad, Calif.), the pcDNA.TM. system (Invitrogen, Inc.,
Carlsbad, Calif.), the pSecTag2 system (Invitrogen, Inc., Carlsbad,
Calif.), the Exchanger.RTM. System, InterPlay.TM. Mammalian TAP
System (Stratagene, La Jolla, Calif.), Complete Control.RTM.
Inducible Mammalian Expression System (Stratagene, La Jolla,
Calif.) and LacSwitch.RTM. II Inducible Mammalian Expression System
(Stratagene, La Jolla, Calif.).
[0754] Another procedure of expressing a modified Clostridial toxin
encoded by polynucleotide molecule disclosed in the present
specification employs a cell-free expression system such as,
without limitation, prokaryotic extracts and eukaryotic extracts.
Non-limiting examples of prokaryotic cell extracts include the RTS
100 E. coli HY Kit (Roche Applied Science, Indianapolis, Ind.), the
ActivePro In Vitro Translation Kit (Ambion, Inc., Austin, Tex.),
the EcoPro.TM. System (EMD Biosciences-Novagen, Madison, Wis.) and
the Expressway.TM. Plus Expression System (Invitrogen, Inc.,
Carlsbad, Calif.). Eukaryotic cell extract include, without
limitation, the RTS 100 Wheat Germ CECF Kit (Roche Applied Science,
Indianapolis, Ind.), the TnT.RTM. Coupled Wheat Germ Extract
Systems (Promega Corp., Madison, Wis.), the Wheat Germ IVT.TM. Kit
(Ambion, Inc., Austin, Tex.), the Retic Lysate IVT.TM. Kit (Ambion,
Inc., Austin, Tex.), the PROTEINscript.RTM. II System (Ambion,
Inc., Austin, Tex.) and the TnT.RTM. Coupled Reticulocyte Lysate
Systems (Promega Corp., Madison, Wis.).
[0755] Aspects of the present invention can also be described as
follows: [0756] 1. A modified Clostridial toxin comprising: [0757]
a) a Clostridial toxin enzymatic domain capable of executing an
enzymatic target modification step of a Clostridial toxin
intoxication process; [0758] b) a Clostridial toxin translocation
domain capable of executing a translocation step of a Clostridial
toxin intoxication process; [0759] c) a translocation facilitating
domain capable of facilitating a translocation step of a
Clostridial toxin intoxication process; [0760] d) an enhanced
targeting domain comprising a modified Clostridial H.sub.CC
targeting domain capable of executing a cell binding step of a
Clostridial toxin intoxication process; and [0761] e) a protease
cleavage site; [0762] wherein cleavage of the protease cleavage
site converts the single-chain form of the modified Clostridial
toxin into the di-chain form; and [0763] wherein the modified
Clostridial H.sub.CC targeting domain exhibits enhanced binding
activity for an endogenous Clostridial toxin receptor relative to
the binding activity of a naturally-occurring Clostridial H.sub.CC
targeting domain from which the modified Clostridial H.sub.CC
targeting domain is derived. [0764] 2. The modified Clostridial
toxin according to 1, wherein the modified Clostridial toxin
comprises, in a linear amino-to-carboxyl single polypeptide order,
the Clostridial toxin enzymatic domain, the protease cleavage site,
the Clostridial toxin translocation domain, the translocation
facilitating domain and the enhanced targeting domain. [0765] 3.
The modified Clostridial toxin according to 1, wherein the modified
Clostridial toxin comprises, in a linear amino-to-carboxyl single
polypeptide order, the Clostridial toxin enzymatic domain, the
protease cleavage site, the enhanced targeting domain, the
Clostridial toxin translocation domain and the translocation
facilitating domain. [0766] 4. The modified Clostridial toxin
according to 1, wherein the modified Clostridial toxin comprises,
in a linear amino-to-carboxyl single polypeptide order, the
enhanced targeting domain, the Clostridial toxin translocation
domain, the translocation facilitating domain, the protease
cleavage site and the Clostridial toxin enzymatic domain. [0767] 5.
The modified Clostridial toxin according to 1, wherein the modified
Clostridial toxin comprises, in a linear amino-to-carboxyl single
polypeptide order, the enhanced targeting domain, the Clostridial
toxin enzymatic domain, the protease cleavage site, the Clostridial
toxin translocation domain and the translocation facilitating
domain. [0768] 6. The modified Clostridial toxin according to 1,
wherein the modified Clostridial toxin comprises, in a linear
amino-to-carboxyl single polypeptide order, the Clostridial toxin
translocation domain, the translocation facilitating domain, the
protease cleavage site, the Clostridial toxin enzymatic domain and
the enhanced targeting domain. [0769] 7. The modified Clostridial
toxin according to 1, wherein the modified Clostridial toxin
comprises, in a linear amino-to-carboxyl single polypeptide order,
the Clostridial toxin translocation domain, the translocation
facilitating domain, the protease cleavage site, the enhanced
targeting domain and the Clostridial toxin enzymatic domain. [0770]
8. The modified Clostridial toxin according to 1, wherein the
Clostridial toxin enzymatic domain is selected from the group
consisting of a BoNT/A enzymatic domain, a BoNT/B enzymatic domain,
a BoNT/C1 enzymatic domain, a BoNT/D enzymatic domain, a BoNT/E
enzymatic domain, a BoNT/F enzymatic domain, a BoNT/G enzymatic
domain and a TeNT enzymatic domain. [0771] 9. The modified
Clostridial toxin according to 1, wherein the Clostridial toxin
translocation domain is selected from the group consisting of a
BoNT/A translocation domain, a BoNT/B translocation domain, a
BoNT/C1 translocation domain, a BoNT/D translocation domain, a
BoNT/E translocation domain, a BoNT/F translocation domain, a
BoNT/G translocation domain and a TeNT translocation domain. [0772]
10. The modified Clostridial toxin according to 1, wherein the
translocation facilitating domain is a Clostridial toxin
translocation facilitating domain. [0773] 11. The modified
Clostridial toxin according to 10, wherein the Clostridial toxin
translocation facilitating domain is selected from the group
consisting of a BoNT/A translocation facilitating domain, a BoNT/B
translocation facilitating domain, a BoNT/C1 translocation
facilitating domain, a BoNT/D translocation domain, a BoNT/E
translocation facilitating domain, a BoNT/F translocation
facilitating domain, a BoNT/G translocation facilitating domain and
a TeNT translocation facilitating domain. [0774] 12. The modified
Clostridial toxin according to 10, wherein the Clostridial toxin
translocation facilitating domain comprises an amino acid sequence
selected from the group consisting of amino acids 874-1110 of SEQ
ID NO: 1, amino acids 861-1097 of SEQ ID NO: 2, amino acids
869-1111 of SEQ ID NO: 3, amino acids 865-1098 of SEQ ID NO: 4,
amino acids 848-1085 of SEQ ID NO: 5, amino acids 867-1105 of SEQ
ID NO: 6, amino acids 866-1105 of SEQ ID NO: 7 and amino acid
882-1127 of SEQ ID NO: 8. [0775] 13. The modified Clostridial toxin
according to 1, wherein the translocation facilitating domain is an
enveloped virus fusogenic peptide domain. [0776] 14. The modified
Clostridial toxin according to 13, wherein the enveloped virus
fusogenic peptide domain is selected from the group consisting of
an influenzavirus fusogenic peptide domain, an alphavirus fusogenic
peptide domain, a vesiculovirus fusogenic peptide domain, a
respirovirus fusogenic peptide domain, a morbillivirus fusogenic
peptide domain, an avulavirus fusogenic peptide domain, a
henipavirus fusogenic peptide domain, a metapneumovirus fusogenic
peptide domain and a foamy virus fusogenic peptide domain. [0777]
15. The modified Clostridial toxin according to 14, wherein the
influenzavirus fusogenic peptide domain is selected from the group
consisting of SEQ ID NO: 87, SEQ ID NO: 88, SEQ ID NO: 89, SEQ ID
NO: 90 and SEQ ID NO: 91. [0778] 16. The modified Clostridial toxin
according to 14, wherein the alphavirus fusogenic peptide domain is
selected from the group consisting of SEQ ID NO: 92, SEQ ID NO: 93,
SEQ ID NO: 94, SEQ ID NO: 95, SEQ ID NO: 96, SEQ ID NO: 97, SEQ ID
NO: 98, SEQ ID NO: 99, SEQ ID NO: 100, SEQ ID NO: 101, SEQ ID NO:
102, SEQ ID NO: 103, SEQ ID NO: 104, SEQ ID NO: 105, SEQ ID NO:
106, SEQ ID NO: 107, SEQ ID NO: 108, SEQ ID NO: 109, SEQ ID NO:
110, SEQ ID NO: 111, SEQ ID NO: 112, SEQ ID NO: 113, SEQ ID NO:
114, SEQ ID NO: 115, SEQ ID NO: 116, SEQ ID NO: 117 and SEQ ID NO:
118. [0779] 17. The modified Clostridial toxin according to 14,
wherein the vesiculovirus fusogenic peptide domain is selected from
the group consisting of SEQ ID NO: 119, SEQ ID NO: 120, SEQ ID NO:
121, SEQ ID NO: 122, SEQ ID NO: 123, SEQ ID NO: 124, SEQ ID NO:
125, SEQ ID NO: 126, SEQ ID NO: 127, SEQ ID NO: 128 and SEQ ID NO:
129. [0780] 18. The modified Clostridial toxin according to 14,
wherein the respirovirus fusogenic peptide domain is selected from
the group consisting of SEQ ID NO: 130, SEQ ID NO: 131, SEQ ID NO:
132, SEQ ID NO: 133, SEQ ID NO: 134, SEQ ID NO: 135 and SEQ ID NO:
136. [0781] 19. The modified Clostridial toxin according to 14,
wherein the morbillivirus fusogenic peptide domain is selected from
the group consisting of SEQ ID NO: 137, SEQ ID NO: 138, SEQ ID NO:
139, SEQ ID NO: 140, SEQ ID NO: 141, SEQ ID NO: 142, SEQ ID NO:
143, SEQ ID NO: 144, SEQ ID NO: 145 and SEQ ID NO: 146. [0782] 20.
The modified Clostridial toxin according to 14, wherein the
avulavirus fusogenic peptide domain is selected from the group
consisting of SEQ ID NO: 147, SEQ ID NO: 148, SEQ ID NO: 149, SEQ
ID NO: 150, SEQ ID NO: 151, SEQ ID NO: 152, SEQ ID NO: 153, SEQ ID
NO: 154, SEQ ID NO: 155, SEQ ID NO: 156, SEQ ID NO: 157, SEQ ID NO:
158 and SEQ ID NO: 159. [0783] 21. The modified Clostridial toxin
according to 14, wherein the henipavirus fusogenic peptide domain
is selected from the group consisting of SEQ ID NO: 160 and SEQ ID
NO: 161. [0784] 22. The modified Clostridial toxin according to 14,
wherein the metapneumovirus fusogenic peptide domain is SEQ ID NO:
162. [0785] 23. The modified Clostridial toxin according to 1,
wherein the modified Clostridial toxin H.sub.CC targeting domain is
selected from the group consisting of a modified BoNT/A H.sub.CC
targeting domain, a modified BoNT/B H.sub.CC targeting domain, a
modified BoNT/C1 H.sub.CC targeting domain, a modified BoNT/D
H.sub.CC targeting domain, a modified BoNT/E H.sub.CC targeting
domain, a modified BoNT/F H.sub.CC targeting domain, a modified
BoNT/G H.sub.CC targeting domain and a modified TeNT H.sub.CC
targeting domain. [0786] 24. The modified Clostridial toxin
according to 23, wherein the modified BoNT/A H.sub.CC targeting
domain comprises an amino acid substitution at a position selected
from the group consisting of Trp 1101, Gly 1102, Leu 1105, Tyr
1111, Tyr 1112, Gly 1158, Ile 1163, Asp 1179, Glu 1203, Phe 1252,
Ser 1264, Trp 1266, Tyr 1267, Gln 1270, Gly 1279 and Trp 1282 of
SEQ ID NO: 1, and any combination thereof; [0787] wherein the amino
acid substitution enhances the binding activity of the modified
BoNT/A H.sub.CC targeting domain for a BoNT/A receptor. [0788] 25.
The modified Clostridial toxin according to 23, wherein the
modified BoNT/B H.sub.CC targeting domain comprises an amino acid
substitution at a position selected from the group consisting of
Trp 1088, Gly 1089, Leu 1092, Tyr 1098, Tyr 1099, Gly 1142, Ile
1147, Asp 1165, Glu 1191, Ile 1240, Ser 1260, Trp 1262, Tyr 1263,
Glu 1266, Gly 1277 and Trp 1280 of SEQ ID NO: 2, and any
combination thereof; [0789] wherein the amino acid substitution
enhances the binding activity of the modified BoNT/B H.sub.CC
targeting domain for a BoNT/B receptor. [0790] 26. The modified
Clostridial toxin according to 23, wherein the modified BoNT/C1
H.sub.CC targeting domain comprises an amino acid substitution at a
position selected from the group consisting of Trp 1102, Gly 1103,
Leu 1106, Tyr 1112, Tyr 1113, Gly 1145, Ile 1150, Asp 1166, Glu
1196, Ile 1247, Gly 1256, Trp 1258, Tyr 1259, H is 1261, Gly 1281
and Trp 1284 of SEQ ID NO: 3, and any combination thereof; [0791]
wherein the amino acid substitution enhances the binding activity
of the modified BoNT/C1 H.sub.CC targeting domain for a BoNT/C1
receptor. [0792] 27. The modified Clostridial toxin according to
23, wherein the modified BoNT/D H.sub.CC targeting domain comprises
an amino acid substitution at a position selected from the group
consisting of Trp 1089, Gly 1090, Leu 1093, Tyr 1099, Tyr 1100, Gly
1132, Ile 1137, Asp 1153, Asn 1186, Lys 1236, Trp 1238, Arg 1239,
Phe 1242, Ser 1262 and Trp 1265 of SEQ ID NO: 4, and any
combination thereof; [0793] wherein the amino acid substitution
enhances the binding activity of the modified BoNT/D H.sub.CC
targeting domain for a BoNT/D receptor. [0794] 28. The modified
Clostridial toxin according to 23, wherein the modified BoNT/E
H.sub.CC targeting domain comprises an amino acid substitution at a
position selected from the group consisting of Trp 1076, Gly 1077,
Leu 1080, Tyr 1086, Tyr 1087, Gly 1124, Ile 1129, Asp 1146, Glu
1172, Phe 1213, Ser 1221, Trp 1223, Tyr 1224, H is 1227, Gly 1236
and Trp 1239 of SEQ ID NO: 5, and any combination thereof; [0795]
wherein the amino acid substitution enhances the binding activity
of the modified BoNT/E H.sub.CC targeting domain for a BoNT/E
receptor. [0796] 29. The modified Clostridial toxin according to
23, wherein the modified BoNT/F H.sub.CC targeting domain comprises
an amino acid substitution at a position selected from the group
consisting of Trp 1096, Gly 1097, Leu 1100, Tyr 1106, Tyr 1107, Gly
1147, Ile 1152, Asp 1171, Glu 1195, Phe 1237, Ser 1245, Trp 1247,
Tyr 1248, Asn 1251, Gly 1260 and Trp 1263 of SEQ ID NO: 6, and any
combination thereof; [0797] wherein the amino acid substitution
enhances the binding activity of the modified BoNT/F H.sub.CC
targeting domain for a BoNT/F receptor. [0798] 30. The modified
Clostridial toxin according to 23, wherein the modified BoNT/G
H.sub.CC targeting domain comprises an amino acid substitution at a
position selected from the group consisting of Trp 1096, Gly 1097,
Leu 1100, Tyr 1106, Tyr 1107, Gly 1148, Ile 1153, Asp 1172, Gln
1198, Ile 1245, Ser 1266, Trp 1268, Tyr 1269, Arg 1272, Gly 1283
and Trp 1285 of SEQ ID NO: 7, and any combination thereof; [0799]
wherein the amino acid substitution enhances the binding activity
of the modified BoNT/G H.sub.CC targeting domain for a BoNT/G
receptor. [0800] 31. The modified Clostridial toxin according to
23, wherein the modified TeNT H.sub.CC targeting domain comprises
an amino acid substitution at a position selected from the group
consisting of Trp 1118, Gly 1119, Leu 1122, Tyr 1128, Tyr 1129, Gly
1172, Ile 1177, Asp 1194, Asp 1222, Thr 1270, Ser 1287, Trp 1289,
Tyr 1290, H is 1293, Gly 1300 and Trp 1303 of SEQ ID NO: 8, and any
combination thereof; [0801] wherein the amino acid substitution
enhances the binding activity of the modified TeNT H.sub.CC
targeting domain for a TeNT receptor. [0802] 32. The modified
Clostridial toxin according to 1, wherein the modified Clostridial
toxin H.sub.CC targeting domain comprises at least one amino acid
substitution in a .beta.-trefoil domain. [0803] 33. The modified
Clostridial toxin according to 31, wherein the amino acid
substitution is located within an .alpha.-fold, a .beta.-fold or a
.gamma.-fold of the .beta.-trefoil domain. [0804] 34. The modified
Clostridial toxin according to 31, wherein the amino acid
substitution is located within a .beta.4/.beta.5 .beta.-hairpin
turn or a .beta.8/.beta.9 .beta.-hairpin turn of the .beta.-trefoil
domain. [0805] 35. The modified Clostridial toxin according to 33,
wherein the amino acid substitution is a glycine or a
phenylalanine. [0806] 36. The modified Clostridial toxin according
to 1, wherein the modified Clostridial toxin H.sub.CC targeting
domain comprises a substitution of a Clostridial toxin H.sub.CC
targeting domain .alpha.-fold for an .alpha.-fold selected from the
group consisting of a Clostridial botulinum serotype A HA-33
1.alpha.-fold, a Clostridial botulinum serotype B HA-33
1.alpha.-fold, a Clostridial botulinum serotype C1 HA-33
1.alpha.-fold, a Clostridial botulinum serotype D HA-33
1.alpha.-fold, a Clostridial botulinum serotype A HA-33
2.alpha.-fold, a Clostridial botulinum serotype B HA-33
2.alpha.-fold, a Clostridial botulinum serotype C1 HA-33
2.alpha.-fold, a Clostridial botulinum serotype D HA-33
2.alpha.-fold, a Clostridial botulinum serotype A HA-17
.alpha.-fold, a Clostridial botulinum serotype B HA-17
.alpha.-fold, a Clostridial botulinum serotype C1 HA-17
.alpha.-fold, a Clostridial botulinum serotype D HA-17
.alpha.-fold, a Clostridial botulinum serotype A NTNH
.alpha.-fold, a Clostridial botulinum serotype B NTNH .alpha.-fold,
a Clostridial botulinum serotype C1 NTNH .alpha.-fold, a
Clostridial botulinum serotype D NTNH .alpha.-fold, a Clostridial
botulinum serotype E NTNH .alpha.-fold, a Clostridial botulinum
serotype F NTNH .alpha.-fold, a Clostridial botulinum serotype G
NTNH .alpha.-fold, a FGF-1.alpha.-fold, a FGF-2 .alpha.-fold, a
FGF-4 .alpha.-fold, a FGF-8 .alpha.-fold, a FGF-9 .alpha.-fold, a
FGF-17 .alpha.-fold and a FGF-18 .alpha.-fold. [0807] 37. The
modified Clostridial toxin according to 1, wherein the modified
Clostridial toxin H.sub.CC targeting domain comprises a
substitution of a Clostridial toxin H.sub.CC targeting domain
.beta.-fold for a .beta.-fold selected from the group consisting of
a Clostridial botulinum serotype A HA-33 1.beta.-fold, a
Clostridial botulinum serotype B HA-33 1.beta.-fold, a Clostridial
botulinum serotype C1 HA-33 1.beta.-fold, a Clostridial botulinum
serotype D HA-33 1.beta.-fold, a Clostridial botulinum serotype A
HA-33 2.beta.-fold, a Clostridial botulinum serotype B HA-33
2.beta.-fold, a Clostridial botulinum serotype C1 HA-33
2.beta.-fold, a Clostridial botulinum serotype D HA-33
2.beta.-fold, a Clostridial botulinum serotype A HA-17
1.beta.-fold, a Clostridial botulinum serotype B HA-17
1.beta.-fold, a Clostridial botulinum serotype C1 HA-17
1.beta.-fold, a Clostridial botulinum serotype D HA-17
1.beta.-fold, a Clostridial botulinum serotype A NTNH .beta.-fold,
a Clostridial botulinum serotype B NTNH .beta.-fold, a Clostridial
botulinum serotype C1 NTNH .beta.-fold, a Clostridial botulinum
serotype D NTNH .beta.-fold, a Clostridial botulinum serotype E
NTNH .beta.-fold, a Clostridial botulinum serotype F NTNH
.beta.-fold, a Clostridial botulinum serotype G NTNH .beta.-fold, a
FGF-1 .beta.-fold, a FGF-2 fold, a FGF-4 .beta.-fold, a FGF-8
.beta.-fold, a FGF-9 .beta.-fold, a FGF-17 .beta.-fold and a FGF-18
.beta.-fold. [0808] 38. The modified Clostridial toxin according to
1, wherein the modified Clostridial toxin H.sub.CC targeting domain
comprises a substitution of a Clostridial toxin H.sub.CC targeting
domain .gamma.-fold for a .gamma.-fold selected from the group
consisting of a Clostridial botulinum serotype A HA-33
1.gamma.-fold, a Clostridial botulinum serotype B HA-33
1.gamma.-fold, a Clostridial botulinum serotype C1 HA-33
1.gamma.-fold, a Clostridial botulinum serotype D HA-33
1.gamma.-fold, a Clostridial botulinum serotype A HA-33
2.gamma.-fold, a Clostridial botulinum serotype B HA-33
2.gamma.-fold, a Clostridial botulinum serotype C1 HA-33
2.gamma.-fold, a Clostridial botulinum serotype D HA-33
2.gamma.-fold, a Clostridial botulinum serotype A HA-17
.gamma.-fold, a Clostridial botulinum serotype B HA-17
.gamma.-fold, a Clostridial botulinum serotype C1 HA-17
.gamma.-fold, a Clostridial botulinum serotype D HA-17
.gamma.-fold, a Clostridial botulinum serotype A NTNH .gamma.-fold,
a Clostridial botulinum serotype B NTNH .gamma.-fold, a Clostridial
botulinum serotype C1 NTNH .gamma.-fold, a Clostridial botulinum
serotype D NTNH .gamma.-fold, a Clostridial botulinum serotype E
NTNH .gamma.-fold, a Clostridial botulinum serotype F NTNH
.gamma.-fold, a Clostridial botulinum serotype G NTNH .gamma.-fold,
a FGF-1 .gamma.-fold, a FGF-2 .gamma.-fold, a FGF-4 .gamma.-fold, a
FGF-8 .gamma.-fold, a FGF-9 .gamma.-fold, a FGF-17 .gamma.-fold and
a FGF-18 .gamma.-fold. [0809] 39. The modified Clostridial toxin
according to 1, wherein the modified Clostridial toxin H.sub.CC
targeting domain comprises a substitution of a Clostridial toxin
H.sub.CC targeting domain .beta.4/.beta.5 .beta.-hairpin turn for a
.beta.4/.beta.5 .beta.-hairpin turn selected from the group
consisting of a Clostridial botulinum serotype A HA-33
.beta.4/.beta.5 .beta.-hairpin turn, a Clostridial botulinum
serotype B HA-33 1.beta.4/.beta.5 .beta.-hairpin turn, a
Clostridial botulinum serotype C1 HA-33 1.beta.4/.beta.5
.beta.-hairpin turn, a Clostridial botulinum serotype D HA-33
1.beta.4/.beta.5 .beta.-hairpin turn, a Clostridial botulinum
serotype A HA-33 2.beta.4/.beta.5 .beta.-hairpin turn, a
Clostridial botulinum serotype B HA-33 2.beta.4/.beta.5
.beta.-hairpin turn, a Clostridial botulinum serotype C1 HA-33
2.beta.4/.beta.5 .beta.-hairpin turn, a Clostridial botulinum
serotype D HA-33 2.beta.4/.beta.5 .beta.-hairpin turn, a
Clostridial botulinum serotype A HA-17 .beta.4/.beta.5
.beta.-hairpin turn, a Clostridial botulinum serotype B HA-17
.beta.4/.beta.5 .beta.-hairpin turn, a Clostridial botulinum
serotype C1 HA-17 .beta.4/.beta.5 .beta.-hairpin turn, a
Clostridial botulinum serotype D HA-17 .beta.4/.beta.5
.beta.-hairpin turn, a Clostridial botulinum serotype A NTNH
.beta.4/.beta.5 .beta.-hairpin turn, a Clostridial botulinum
serotype B NTNH 134435.beta.-hairpin turn, a Clostridial botulinum
serotype C1 NTNH .beta.4/.beta.5 .beta.-hairpin turn, a Clostridial
botulinum serotype D NTNH .beta.4/.beta.5 .beta.-hairpin turn, a
Clostridial botulinum serotype E NTNH .beta.4/.beta.5
.beta.-hairpin turn, a Clostridial botulinum serotype F NTNH
.beta.4/.beta.5 .beta.-hairpin turn, a Clostridial botulinum
serotype G NTNH 134435.beta.-hairpin turn, a FGF-1 .beta.4/.beta.5
.beta.-hairpin turn, a FGF-2 .beta.4/.beta.5 .beta.-hairpin turn, a
FGF-4 .beta.4/.beta.5 .beta.-hairpin turn, a FGF-8 .beta.4/.beta.5
.beta.-hairpin turn, a FGF-9 .beta.4/.beta.5 .beta.-hairpin turn, a
FGF-17 .beta.4/.beta.5 .beta.-hairpin turn and a FGF-18
.beta.4/.beta.5 .beta.-hairpin turn. [0810] 40. The modified
Clostridial toxin according to 1, wherein the modified Clostridial
toxin H.sub.CC targeting domain comprises a substitution of a
Clostridial toxin H.sub.CC targeting domain .beta.8/.beta.9
.beta.-hairpin turn for a .beta.8/.beta.9 .beta.-hairpin turn
selected from the group consisting of a Clostridial botulinum
serotype A HA-33 .beta.8/.beta.9 .beta.-hairpin turn, a Clostridial
botulinum serotype B HA-33 1.beta.8/.beta.9 .beta.-hairpin turn, a
Clostridial botulinum serotype C1 HA-33 1.beta.8/.beta.9
.beta.-hairpin turn, a Clostridial botulinum serotype D HA-33
1.beta.8/.beta.9 .beta.-hairpin turn, a Clostridial botulinum
serotype A HA-33 2.beta.8/.beta.9 .beta.-hairpin turn, a
Clostridial botulinum serotype B HA-33 2.beta.8/.beta.9
.beta.-hairpin turn, a Clostridial botulinum serotype C1 HA-33
2.beta.8/.beta.9 .beta.-hairpin turn, a Clostridial botulinum
serotype D HA-33 2.beta.8/.beta.9 .beta.-hairpin turn, a
Clostridial botulinum serotype A HA-17 .beta.8/.beta.9
.beta.-hairpin turn, a Clostridial botulinum serotype B HA-17
.beta.8/.beta.9 .beta.-hairpin turn, a Clostridial botulinum
serotype C1 HA-17 .beta.8/.beta.9 .beta.-hairpin turn, a
Clostridial botulinum serotype D HA-17 .beta.8/.beta.9
.beta.-hairpin turn, a Clostridial botulinum serotype A NTNH
.beta.8/.beta.9 .beta.-hairpin turn, a Clostridial botulinum
serotype B NTNH .beta.8/.beta.9 .beta.-hairpin turn, a Clostridial
botulinum serotype C1 NTNH .beta.8/.beta.9 .beta.-hairpin turn, a
Clostridial botulinum serotype D NTNH .beta.8/.beta.9
.beta.-hairpin turn, a Clostridial botulinum serotype E NTNH
.beta.8/.beta.9 .beta.-hairpin turn, a Clostridial botulinum
serotype F NTNH .beta.8/.beta.9 .beta.-hairpin turn, a Clostridial
botulinum serotype G NTNH .beta.8/.beta.9 .beta.-hairpin turn, a
FGF-1 .beta.8/.beta.9 .beta.-hairpin turn, a FGF-2 .beta.8/.beta.9
.beta.-hairpin turn, a FGF-4 .beta.8/.beta.9 .beta.-hairpin turn, a
FGF-8 .beta.8/.beta.9 .beta.-hairpin turn, a FGF-9 .beta.8/.beta.9
.beta.-hairpin turn, a FGF-17 .beta.8/.beta.9 .beta.-hairpin turn
and a FGF-18 .beta.8/.beta.9 .beta.-hairpin turn. [0811] 41. The
modified Clostridial toxin according to 1, wherein the protease
cleavage site is an endogenous Clostridial toxin di-chain loop
protease cleavage site or an exogenous cleavage site. [0812] 42.
The modified Clostridial toxin according to 41, wherein the
endogenous Clostridial toxin di-chain loop protease cleavage site
is selected from the group consisting of a BoNT/A di-chain loop
protease cleavage site, a BoNT/B di-chain loop protease cleavage
site, a BoNT/C1 di-chain loop protease cleavage site, a BoNT/D
di-chain loop protease cleavage site, a BoNT/E di-chain loop
protease cleavage site, a BoNT/F di-chain loop protease cleavage
site, a BoNT/G di-chain loop protease cleavage site and a TeNT
di-chain loop protease cleavage site. [0813] 43. The modified
Clostridial toxin according to 41, wherein the exogenous protease
cleavage site is selected from the group consisting of an
enterokinase cleavage site, a Thrombin cleavage site, a Factor Xa
cleavage site, a human rhinovirus 3C protease cleavage site, a
tobacco etch virus protease cleavage site, a dipeptidyl
aminopeptidase cleavage site, a small ubiquitin-like modifier
(SUMO)/ubiquitin-like protein-1 (ULP-1) protease cleavage site, and
a Clostridial toxin substrate cleavage site. [0814] 44. The
modified Clostridial toxin according to 43, wherein the Clostridial
toxin substrate cleavage site is selected from the group consisting
of a BoNT/A substrate cleavage site, a BoNT/B substrate cleavage
site, a BoNT/C1 substrate cleavage site, a BoNT/D substrate
cleavage site, a BoNT/E substrate cleavage site, a BoNT/F substrate
cleavage site, a BoNT/G substrate cleavage site and a TeNT
substrate cleavage site. [0815] 45. A modified Clostridial toxin
comprising: [0816] a) a Clostridial toxin enzymatic domain capable
of executing an enzymatic target modification step of a Clostridial
toxin intoxication process; [0817] b) a Clostridial toxin
translocation domain capable of executing a translocation step of a
Clostridial toxin intoxication process; [0818] c) a translocation
facilitating domain capable of facilitating a translocation step of
a Clostridial toxin intoxication process; [0819] d) an enhanced
targeting domain comprising a Clostridial non-toxin associated
protein .beta.-trefoil domain capable of executing a cell binding
step of a Clostridial toxin intoxication process; and [0820] e) a
protease cleavage site [0821] wherein cleavage of the protease
cleavage site converts the single-chain form of the modified
Clostridial toxin into the di-chain form. [0822] 46. The modified
Clostridial toxin according to 45, wherein the modified Clostridial
toxin comprises, in a linear amino-to-carboxyl single polypeptide
order, the Clostridial toxin enzymatic domain, the protease
cleavage site, the Clostridial toxin translocation domain, the
translocation facilitating domain and the enhanced targeting
domain. [0823] 47. The modified Clostridial toxin according to 45,
wherein the modified Clostridial toxin comprises, in a linear
amino-to-carboxyl single polypeptide order, the Clostridial toxin
enzymatic domain, the protease cleavage site, the enhanced
targeting domain, the Clostridial toxin translocation domain and
the translocation facilitating domain. [0824] 48. The modified
Clostridial toxin according to 45, wherein the modified Clostridial
toxin comprises, in a linear amino-to-carboxyl single polypeptide
order, the enhanced targeting domain, the Clostridial toxin
translocation domain, the translocation facilitating domain, the
protease cleavage site and the Clostridial toxin enzymatic domain.
[0825] 49. The modified Clostridial toxin according to 45, wherein
the modified Clostridial toxin comprises, in a linear
amino-to-carboxyl single polypeptide order, the enhanced targeting
domain, the Clostridial toxin enzymatic domain, the protease
cleavage site, the Clostridial toxin translocation domain and the
translocation facilitating domain. [0826] 50. The modified
Clostridial toxin according to 45, wherein the modified Clostridial
toxin comprises, in a linear amino-to-carboxyl single polypeptide
order, the Clostridial toxin translocation domain, the
translocation facilitating domain, the protease cleavage site, the
Clostridial toxin enzymatic domain and the enhanced targeting
domain. [0827] 51. The modified Clostridial toxin according to 45,
wherein the modified Clostridial toxin comprises, in a linear
amino-to-carboxyl single polypeptide order, the Clostridial toxin
translocation domain, the translocation facilitating domain, the
protease cleavage site, the enhanced targeting domain and the
Clostridial toxin enzymatic domain. [0828] 52. The modified
Clostridial toxin according to 45, wherein the Clostridial toxin
enzymatic domain is selected from the group consisting of a BoNT/A
enzymatic domain, a BoNT/B enzymatic domain, a BoNT/C1 enzymatic
domain, a BoNT/D enzymatic domain, a BoNT/E enzymatic domain, a
BoNT/F enzymatic domain, a BoNT/G enzymatic domain and a TeNT
enzymatic domain. [0829] 53. The modified Clostridial toxin
according to 45, wherein the Clostridial toxin translocation domain
is selected from the group consisting of a BoNT/A translocation
domain, a BoNT/B translocation domain, a BoNT/C1 translocation
domain, a BoNT/D translocation domain, a BoNT/E translocation
domain, a BoNT/F translocation domain, a BoNT/G translocation
domain and a TeNT translocation domain. [0830] 54. The modified
Clostridial toxin according to 45, wherein the translocation
facilitating domain is a Clostridial toxin translocation
facilitating domain. [0831] 55. The modified Clostridial toxin
according to 54, wherein the Clostridial toxin translocation
facilitating domain is selected from the group consisting of a
BoNT/A translocation facilitating domain, a BoNT/B translocation
facilitating domain, a BoNT/C1 translocation facilitating domain, a
BoNT/D translocation domain, a BoNT/E translocation facilitating
domain, a BoNT/F translocation facilitating domain, a BoNT/G
translocation facilitating domain and a TeNT translocation
facilitating domain. [0832] 56. The modified Clostridial toxin
according to 54, wherein the Clostridial toxin translocation
facilitating domain comprises an amino acid sequence selected from
the group consisting of amino acids 874-1110 of SEQ ID NO: 1, amino
acids 861-1097 of SEQ ID NO: 2, amino acids 869-1111 of SEQ ID NO:
3, amino acids 865-1098 of SEQ ID NO: 4, amino acids 848-1085 of
SEQ ID NO: 5, amino acids 867-1105 of SEQ ID NO: 6, amino acids
866-1105 of SEQ ID NO: 7 and amino acid 882-1127 of SEQ ID NO: 8.
[0833] 57. The modified Clostridial toxin according to 45, wherein
the translocation facilitating domain is an enveloped virus
fusogenic peptide domain. [0834] 58. The modified Clostridial toxin
according to 57, wherein the enveloped virus fusogenic peptide
domain is selected from the group consisting of an influenzavirus
fusogenic peptide domain, an alphavirus fusogenic peptide domain, a
vesiculovirus fusogenic peptide domain, a respirovirus fusogenic
peptide domain, a morbillivirus fusogenic peptide domain, an
avulavirus fusogenic peptide domain, a henipavirus fusogenic
peptide domain, a metapneumovirus fusogenic peptide domain and a
foamy virus fusogenic peptide domain. [0835] 59. The modified
Clostridial toxin according to 58, wherein the influenzavirus
fusogenic peptide domain is selected from the group consisting of
SEQ ID NO: 87, SEQ ID NO: 88, SEQ ID NO: 89, SEQ ID NO: 90 and SEQ
ID NO: 91.
[0836] 60. The modified Clostridial toxin according to 58, wherein
the alphavirus fusogenic peptide domain is selected from the group
consisting of SEQ ID NO: 92, SEQ ID NO: 93, SEQ ID NO: 94, SEQ ID
NO: 95, SEQ ID NO: 96, SEQ ID NO: 97, SEQ ID NO: 98, SEQ ID NO: 99,
SEQ ID NO: 100, SEQ ID NO: 101, SEQ ID NO: 102, SEQ ID NO: 103, SEQ
ID NO: 104, SEQ ID NO: 105, SEQ ID NO: 106, SEQ ID NO: 107, SEQ ID
NO: 108, SEQ ID NO: 109, SEQ ID NO: 110, SEQ ID NO: 111, SEQ ID NO:
112, SEQ ID NO: 113, SEQ ID NO: 114, SEQ ID NO: 115, SEQ ID NO:
116, SEQ ID NO: 117 and SEQ ID NO: 118. [0837] 61. The modified
Clostridial toxin according to 58, wherein the vesiculovirus
fusogenic peptide domain is selected from the group consisting of
SEQ ID NO: 119, SEQ ID NO: 120, SEQ ID NO: 121, SEQ ID NO: 122, SEQ
ID NO: 123, SEQ ID NO: 124, SEQ ID NO: 125, SEQ ID NO: 126, SEQ ID
NO: 127, SEQ ID NO: 128 and SEQ ID NO: 129. [0838] 62. The modified
Clostridial toxin according to 58, wherein the respirovirus
fusogenic peptide domain is selected from the group consisting of
SEQ ID NO: 130, SEQ ID NO: 131, SEQ ID NO: 132, SEQ ID NO: 133, SEQ
ID NO: 134, SEQ ID NO: 135 and SEQ ID NO: 136. [0839] 63. The
modified Clostridial toxin according to 58, wherein the
morbillivirus fusogenic peptide domain is selected from the group
consisting of SEQ ID NO: 137, SEQ ID NO: 138, SEQ ID NO: 139, SEQ
ID NO: 140, SEQ ID NO: 141, SEQ ID NO: 142, SEQ ID NO: 143, SEQ ID
NO: 144, SEQ ID NO: 145 and SEQ ID NO: 146. [0840] 64. The modified
Clostridial toxin according to 58, wherein the avulavirus fusogenic
peptide domain is selected from the group consisting of SEQ ID NO:
147, SEQ ID NO: 148, SEQ ID NO: 149, SEQ ID NO: 150, SEQ ID NO:
151, SEQ ID NO: 152, SEQ ID NO: 153, SEQ ID NO: 154, SEQ ID NO:
155, SEQ ID NO: 156, SEQ ID NO: 157, SEQ ID NO: 158 and SEQ ID NO:
159. [0841] 65. The modified Clostridial toxin according to 58,
wherein the henipavirus fusogenic peptide domain is selected from
the group consisting of SEQ ID NO: 160 and SEQ ID NO: 161. [0842]
66. The modified Clostridial toxin according to 58, wherein the
metapneumovirus fusogenic peptide domain is SEQ ID NO: 162. [0843]
67. The modified Clostridial toxin according to 45, wherein the
Clostridial non-toxin associated protein .beta.-trefoil domain is
selected from the group consisting of a Clostridial botulinum
serotype A HA-33 .beta.-trefoil domain, a Clostridial botulinum
serotype B HA-33 .beta.-trefoil domain, a Clostridial botulinum
serotype C1 HA-33 .beta.-trefoil domain, a Clostridial botulinum
serotype D HA-33 .beta.-trefoil domain, a Clostridial botulinum
serotype A HA-17 .beta.-trefoil domain, a Clostridial botulinum
serotype B HA-17 .beta.-trefoil domain, a Clostridial botulinum
serotype C1 HA-17 .beta.-trefoil domain, a Clostridial botulinum
serotype D HA-17 .beta.-trefoil domain, a Clostridial botulinum
serotype A NTNH .beta.-trefoil domain, a Clostridial botulinum
serotype B NTNH .beta.-trefoil domain, a Clostridial botulinum
serotype C1 NTNH trefoil domain, a Clostridial botulinum serotype D
NTNH .beta.-trefoil domain, a Clostridial botulinum serotype E NTNH
.beta.-trefoil domain, a Clostridial botulinum serotype F NTNH
.beta.-trefoil domain and a Clostridial botulinum serotype G NTNH
.beta.-trefoil domain. [0844] 68. The modified Clostridial toxin
according to 45, wherein the Clostridial non-toxin associated
protein .beta.-trefoil domain is selected from the group consisting
of amino acids 10-144 of SEQ ID NO: 9, amino acids 151-293 of SEQ
ID NO: 9, amino acids 10-144 of SEQ ID NO: 10, amino acids 151-293
of SEQ ID NO: 10, amino acids 10-144 of SEQ ID NO: 11, amino acids
151-293 of SEQ ID NO: 11, amino acids 10-146 of SEQ ID NO: 12,
amino acids 153-294 of SEQ ID NO: 12, amino acids 10-144 of SEQ ID
NO: 13, amino acids 151-279 of SEQ ID NO: 13, amino acids 10-144 of
SEQ ID NO: 14, amino acids 151-292 of SEQ ID NO: 14, amino acids
10-146 of SEQ ID NO: 15, amino acids 153-291 of SEQ ID NO: 15,
amino acids 10-141 of SEQ ID NO: 16, amino acids 148-285 of SEQ ID
NO: 15, amino acids 10-141 of SEQ ID NO: 16, amino acids 148-285 of
SEQ ID NO: 16, amino acids 10-141 of SEQ ID NO: 17, amino acids
148-286 of SEQ ID NO: 17, amino acids 10-141 of SEQ ID NO: 18,
amino acids 148-286 of SEQ ID NO: 18, amino acids 9-146 of SEQ ID
NO: 19, amino acids 9-146 of SEQ ID NO: 20, amino acids 9-146 of
SEQ ID NO: 21, amino acids 9-146 of SEQ ID NO: 22, amino acids
1050-1194 of SEQ ID NO: 23, amino acids 1050-1199 of SEQ ID NO: 24,
amino acids 1050-1194 of SEQ ID NO: 25, amino acids 1049-1198 of
SEQ ID NO: 26, amino acids 1049-1197 of SEQ ID NO: 27, amino acids
1049-1197 of SEQ ID NO: 28, amino acids 1014-1163 of SEQ ID NO: 29,
amino acids 1016-1160 of SEQ ID NO: 30, amino acids 1017-1166 of
SEQ ID NO: 31 and amino acids 1050-1197 of SEQ ID NO: 1199. [0845]
69. The modified Clostridial toxin according to 45, wherein the
protease cleavage site is an endogenous Clostridial toxin di-chain
loop protease cleavage site or an exogenous cleavage site. [0846]
70. The modified Clostridial toxin according to 69, wherein the
endogenous Clostridial toxin di-chain loop protease cleavage site
is selected from the group consisting of a BoNT/A di-chain loop
protease cleavage site, a BoNT/B di-chain loop protease cleavage
site, a BoNT/C1 di-chain loop protease cleavage site, a BoNT/D
di-chain loop protease cleavage site, a BoNT/E di-chain loop
protease cleavage site, a BoNT/F di-chain loop protease cleavage
site, a BoNT/G di-chain loop protease cleavage site and a TeNT
di-chain loop protease cleavage site. [0847] 71. The modified
Clostridial toxin according to 69, wherein the exogenous protease
cleavage site is selected from the group consisting of an
enterokinase cleavage site, a Thrombin cleavage site, a Factor Xa
cleavage site, a human rhinovirus 3C protease cleavage site, a
tobacco etch virus protease cleavage site, a dipeptidyl
aminopeptidase cleavage site, a small ubiquitin-like modifier
(SUMO)/ubiquitin-like protein-1 (ULP-1) protease cleavage site, and
a Clostridial toxin substrate cleavage site. [0848] 72. The
modified Clostridial toxin according to 71, wherein the Clostridial
toxin substrate cleavage site is selected from the group consisting
of a BoNT/A substrate cleavage site, a BoNT/B substrate cleavage
site, a BoNT/C1 substrate cleavage site, a BoNT/D substrate
cleavage site, a BoNT/E substrate cleavage site, a BoNT/F substrate
cleavage site, a BoNT/G substrate cleavage site and a TeNT
substrate cleavage site. [0849] 73. A modified Clostridial toxin
comprising: [0850] a) a Clostridial toxin enzymatic domain capable
of executing an enzymatic target modification step of a Clostridial
toxin intoxication process; [0851] b) a Clostridial toxin
translocation domain capable of executing a translocation step of a
Clostridial toxin intoxication process; [0852] c) a translocation
facilitating domain capable of facilitating a translocation step of
a Clostridial toxin intoxication process; [0853] d) an enhanced
targeting domain comprising a FGF .beta.-trefoil domain capable of
selectively binding an FGFR3; and [0854] e) a protease cleavage
site [0855] wherein cleavage of the protease cleavage site converts
the single-chain form of the modified Clostridial toxin into the
di-chain form. [0856] 74. The modified Clostridial toxin according
to 73, wherein the modified Clostridial toxin comprises, in a
linear amino-to-carboxyl single polypeptide order, the Clostridial
toxin enzymatic domain, the protease cleavage site, the Clostridial
toxin translocation domain, the translocation facilitating domain
and the enhanced targeting domain. [0857] 75. The modified
Clostridial toxin according to 73, wherein the modified Clostridial
toxin comprises, in a linear amino-to-carboxyl single polypeptide
order, the Clostridial toxin enzymatic domain, the protease
cleavage site, the enhanced targeting domain, the Clostridial toxin
translocation domain and the translocation facilitating domain.
[0858] 76. The modified Clostridial toxin according to 73, wherein
the modified Clostridial toxin comprises, in a linear
amino-to-carboxyl single polypeptide order, the enhanced targeting
domain, the Clostridial toxin translocation domain, the
translocation facilitating domain, the protease cleavage site and
the Clostridial toxin enzymatic domain. [0859] 77. The modified
Clostridial toxin according to 73, wherein the modified Clostridial
toxin comprises, in a linear amino-to-carboxyl single polypeptide
order, the enhanced targeting domain, the Clostridial toxin
enzymatic domain, the protease cleavage site, the Clostridial toxin
translocation domain and the translocation facilitating domain.
[0860] 78. The modified Clostridial toxin according to 73, wherein
the modified Clostridial toxin comprises, in a linear
amino-to-carboxyl single polypeptide order, the Clostridial toxin
translocation domain, the translocation facilitating domain, the
protease cleavage site, the Clostridial toxin enzymatic domain and
the enhanced targeting domain. [0861] 79. The modified Clostridial
toxin according to 73, wherein the modified Clostridial toxin
comprises, in a linear amino-to-carboxyl single polypeptide order,
the Clostridial toxin translocation domain, the translocation
facilitating domain, the protease cleavage site, the enhanced
targeting domain and the Clostridial toxin enzymatic domain. [0862]
80. The modified Clostridial toxin according to 73, wherein the
Clostridial toxin enzymatic domain is selected from the group
consisting of a BoNT/A enzymatic domain, a BoNT/B enzymatic domain,
a BoNT/C1 enzymatic domain, a BoNT/D enzymatic domain, a BoNT/E
enzymatic domain, a BoNT/F enzymatic domain, a BoNT/G enzymatic
domain and a TeNT enzymatic domain. [0863] 81. The modified
Clostridial toxin according to 73, wherein the Clostridial toxin
translocation domain is selected from the group consisting of a
BoNT/A translocation domain, a BoNT/B translocation domain, a
BoNT/C1 translocation domain, a BoNT/D translocation domain, a
BoNT/E translocation domain, a BoNT/F translocation domain, a
BoNT/G translocation domain and a TeNT translocation domain. [0864]
82. The modified Clostridial toxin according to 73, wherein the
translocation facilitating domain is a Clostridial toxin
translocation facilitating domain. [0865] 83. The modified
Clostridial toxin according to 82, wherein the Clostridial toxin
translocation facilitating domain is selected from the group
consisting of a BoNT/A translocation facilitating domain, a BoNT/B
translocation facilitating domain, a BoNT/C1 translocation
facilitating domain, a BoNT/D translocation domain, a BoNT/E
translocation facilitating domain, a BoNT/F translocation
facilitating domain, a BoNT/G translocation facilitating domain and
a TeNT translocation facilitating domain. [0866] 84. The modified
Clostridial toxin according to 82, wherein the Clostridial toxin
translocation facilitating domain comprises an amino acid sequence
selected from the group consisting of amino acids 874-1110 of SEQ
ID NO: 1, amino acids 861-1097 of SEQ ID NO: 2, amino acids
869-1111 of SEQ ID NO: 3, amino acids 865-1098 of SEQ ID NO: 4,
amino acids 848-1085 of SEQ ID NO: 5, amino acids 867-1105 of SEQ
ID NO: 6, amino acids 866-1105 of SEQ ID NO: 7 and amino acid
882-1127 of SEQ ID NO: 8. [0867] 85. The modified Clostridial toxin
according to 73, wherein the translocation facilitating domain is
an enveloped virus fusogenic peptide domain. [0868] 86. The
modified Clostridial toxin according to 85, wherein the enveloped
virus fusogenic peptide domain is selected from the group
consisting of an influenzavirus fusogenic peptide domain, an
alphavirus fusogenic peptide domain, a vesiculovirus fusogenic
peptide domain, a respirovirus fusogenic peptide domain, a
morbillivirus fusogenic peptide domain, an avulavirus fusogenic
peptide domain, a henipavirus fusogenic peptide domain, a
metapneumovirus fusogenic peptide domain and a foamy virus
fusogenic peptide domain. [0869] 87. The modified Clostridial toxin
according to 86, wherein the influenzavirus fusogenic peptide
domain is selected from the group consisting of SEQ ID NO: 87, SEQ
ID NO: 88, SEQ ID NO: 89, SEQ ID NO: 90 and SEQ ID NO: 91. [0870]
88. The modified Clostridial toxin according to 86, wherein the
alphavirus fusogenic peptide domain is selected from the group
consisting of SEQ ID NO: 92, SEQ ID NO: 93, SEQ ID NO: 94, SEQ ID
NO: 95, SEQ ID NO: 96, SEQ ID NO: 97, SEQ ID NO: 98, SEQ ID NO: 99,
SEQ ID NO: 100, SEQ ID NO: 101, SEQ ID NO: 102, SEQ ID NO: 103, SEQ
ID NO: 104, SEQ ID NO: 105, SEQ ID NO: 106, SEQ ID NO: 107, SEQ ID
NO: 108, SEQ ID NO: 109, SEQ ID NO: 110, SEQ ID NO: 111, SEQ ID NO:
112, SEQ ID NO: 113, SEQ ID NO: 114, SEQ ID NO: 115, SEQ ID NO:
116, SEQ ID NO: 117 and SEQ ID NO: 118. [0871] 89. The modified
Clostridial toxin according to 86, wherein the vesiculovirus
fusogenic peptide domain is selected from the group consisting of
SEQ ID NO: 119, SEQ ID NO: 120, SEQ ID NO: 121, SEQ ID NO: 122, SEQ
ID NO: 123, SEQ ID NO: 124, SEQ ID NO: 125, SEQ ID NO: 126, SEQ ID
NO: 127, SEQ ID NO: 128 and SEQ ID NO: 129. [0872] 90. The modified
Clostridial toxin according to 86, wherein the respirovirus
fusogenic peptide domain is selected from the group consisting of
SEQ ID NO: 130, SEQ ID NO: 131, SEQ ID NO: 132, SEQ ID NO: 133, SEQ
ID NO: 134, SEQ ID NO: 135 and SEQ ID NO: 136. [0873] 91. The
modified Clostridial toxin according to 86, wherein the
morbillivirus fusogenic peptide domain is selected from the group
consisting of SEQ ID NO: 137, SEQ ID NO: 138, SEQ ID NO: 139, SEQ
ID NO: 140, SEQ ID NO: 141, SEQ ID NO: 142, SEQ ID NO: 143, SEQ ID
NO: 144, SEQ ID NO: 145 and SEQ ID NO: 146. [0874] 92. The modified
Clostridial toxin according to 86, wherein the avulavirus fusogenic
peptide domain is selected from the group consisting of SEQ ID NO:
147, SEQ ID NO: 148, SEQ ID NO: 149, SEQ ID NO: 150, SEQ ID NO:
151, SEQ ID NO: 152, SEQ ID NO: 153, SEQ ID NO: 154, SEQ ID NO:
155, SEQ ID NO: 156, SEQ ID NO: 157, SEQ ID NO: 158 and SEQ ID NO:
159. [0875] 93. The modified Clostridial toxin according to 86,
wherein the henipavirus fusogenic peptide domain is selected from
the group consisting of SEQ ID NO: 160 and SEQ ID NO: 161. [0876]
94. The modified Clostridial toxin according to 86, wherein the
metapneumovirus fusogenic peptide domain is SEQ ID NO: 162. [0877]
95. The modified Clostridial toxin according to 73, wherein the FGF
.beta.-trefoil domain is selected from the group consisting of a
FGF-1 .beta.-trefoil domain, a FGF-2 .beta.-trefoil domain, a FGF-4
.beta.-trefoil domain, a FGF-8 .beta.-trefoil domain, a FGF-9
.beta.-trefoil domain, a FGF-17 .beta.-trefoil domain and a FGF-18
.beta.-trefoil domain. [0878] 96. The modified Clostridial toxin
according to 73, wherein the FGF .beta.-trefoil domain is selected
from the group consisting of amino acids 26-155 of SEQ ID NO: 33,
amino acids 29-155 of SEQ ID NO: 34, amino acids 83-206 of SEQ ID
NO: 35, amino acids 43-172 of SEQ ID NO: 36, amino acids 63-196 of
SEQ ID NO: 37, amino acids 55-183 of SEQ ID NO: 38 and amino acids
54-183 of SEQ ID NO: 39.
[0879] 97. The modified Clostridial toxin according to 73, wherein
the protease cleavage site is an endogenous Clostridial toxin
di-chain loop protease cleavage site or an exogenous cleavage site.
[0880] 98. The modified Clostridial toxin according to 97, wherein
the endogenous Clostridial toxin di-chain loop protease cleavage
site is selected from the group consisting of a BoNT/A di-chain
loop protease cleavage site, a BoNT/B di-chain loop protease
cleavage site, a BoNT/C1 di-chain loop protease cleavage site, a
BoNT/D di-chain loop protease cleavage site, a BoNT/E di-chain loop
protease cleavage site, a BoNT/F di-chain loop protease cleavage
site, a BoNT/G di-chain loop protease cleavage site and a TeNT
di-chain loop protease cleavage site. [0881] 99. The modified
Clostridial toxin according to 97, wherein the exogenous protease
cleavage site is selected from the group consisting of an
enterokinase cleavage site, a Thrombin cleavage site, a Factor Xa
cleavage site, a human rhinovirus 3C protease cleavage site, a
tobacco etch virus protease cleavage site, a dipeptidyl
aminopeptidase cleavage site, a small ubiquitin-like modifier
(SUMO)/ubiquitin-like protein-1 (ULP-1) protease cleavage site, and
a Clostridial toxin substrate cleavage site. [0882] 100. The
modified Clostridial toxin according to 99, wherein the Clostridial
toxin substrate cleavage site is selected from the group consisting
of a BoNT/A substrate cleavage site, a BoNT/B substrate cleavage
site, a BoNT/C1 substrate cleavage site, a BoNT/D substrate
cleavage site, a BoNT/E substrate cleavage site, a BoNT/F substrate
cleavage site, a BoNT/G substrate cleavage site and a TeNT
substrate cleavage site. [0883] 101. A polynucleotide molecule
encoding a modified Clostridial toxin, the polynucleotide molecule
comprising: [0884] a) a polynucleotide molecule encoding a
Clostridial toxin enzymatic domain capable of executing an
enzymatic target modification step of a Clostridial toxin
intoxication process; [0885] b) a polynucleotide molecule encoding
a Clostridial toxin translocation domain capable of executing a
translocation step of a Clostridial toxin intoxication process;
[0886] c) a polynucleotide molecule encoding a translocation
facilitating domain capable of facilitating a translocation step of
a Clostridial toxin intoxication process; [0887] d) a
polynucleotide molecule encoding an enhanced targeting domain
comprising a modified Clostridial H.sub.CC targeting domain capable
of executing a cell binding step of a Clostridial toxin
intoxication process; and [0888] e) a polynucleotide molecule
encoding a protease cleavage site [0889] wherein cleavage of the
protease cleavage site converts the single-chain form of the
modified Clostridial toxin into the di-chain form; and [0890]
wherein the modified Clostridial H.sub.CC targeting domain exhibits
enhanced binding activity for an endogenous Clostridial toxin
receptor relative to the binding activity of a naturally-occurring
Clostridial H.sub.CC targeting domain from which the modified
Clostridial H.sub.CC targeting domain is derived. [0891] 102. The
polynucleotide molecule according to 101, wherein the
polynucleotide molecule encodes the modified Clostridial toxin of
any one of claims 1-7. [0892] 103. The polynucleotide molecule
according to 101, wherein the polynucleotide molecule encoding a
Clostridial toxin enzymatic domain is selected from the group
consisting of a polynucleotide molecule encoding a BoNT/A enzymatic
domain, a BoNT/B enzymatic domain, a BoNT/C1 enzymatic domain, a
BoNT/D enzymatic domain, a BoNT/E enzymatic domain, a BoNT/F
enzymatic domain, a BoNT/G enzymatic domain and a TeNT enzymatic
domain. [0893] 104. The polynucleotide molecule according to 101,
wherein the polynucleotide molecule encoding a Clostridial toxin
translocation domain is selected from the group consisting of a
polynucleotide molecule encoding a BoNT/A translocation domain, a
BoNT/B translocation domain, a BoNT/C1 translocation domain, a
BoNT/D translocation domain, a BoNT/E translocation domain, a
BoNT/F translocation domain, a BoNT/G translocation domain and a
TeNT translocation domain. [0894] 105. The polynucleotide molecule
according to 101, wherein the translocation facilitating domain is
a Clostridial toxin translocation facilitating domain. [0895] 106.
The polynucleotide molecule according to 105, wherein the
Clostridial toxin translocation facilitating domain is selected
from the group consisting of a BoNT/A translocation facilitating
domain, a BoNT/B translocation facilitating domain, a BoNT/C1
translocation facilitating domain, a BoNT/D translocation domain, a
BoNT/E translocation facilitating domain, a BoNT/F translocation
facilitating domain, a BoNT/G translocation facilitating domain and
a TeNT translocation facilitating domain. [0896] 107. The
polynucleotide molecule according to 101, wherein the translocation
facilitating domain is an enveloped virus fusogenic peptide domain.
[0897] 108. The polynucleotide molecule according to 107, wherein
the enveloped virus fusogenic peptide domain is selected from the
group consisting of an influenzavirus fusogenic peptide domain, an
alphavirus fusogenic peptide domain, a vesiculovirus fusogenic
peptide domain, a respirovirus fusogenic peptide domain, a
morbillivirus fusogenic peptide domain, an avulavirus fusogenic
peptide domain, a henipavirus fusogenic peptide domain, a
metapneumovirus fusogenic peptide domain and a foamy virus
fusogenic peptide domain. [0898] 109. The polynucleotide molecule
according to 101, wherein the polynucleotide molecule encoding a
modified Clostridial toxin H.sub.CC targeting domain is selected
from the group consisting of a polynucleotide molecule encoding a
modified BoNT/A H.sub.CC targeting domain, a modified BoNT/B
H.sub.CC targeting domain, a modified BoNT/C1 H.sub.CC targeting
domain, a modified BoNT/D H.sub.CC targeting domain, a modified
BoNT/E H.sub.CC targeting domain, a modified BoNT/F H.sub.CC
targeting domain, a modified BoNT/G H.sub.CC targeting domain and a
modified TeNT H.sub.CC targeting domain. [0899] 110. The
polynucleotide molecule according to 101, wherein the
polynucleotide molecule encoding a modified Clostridial toxin
H.sub.CC targeting domain comprises a polynucleotide molecule
encoding a substitution of a Clostridial toxin H.sub.CC targeting
domain .alpha.-fold for an .alpha.-fold selected from the group
consisting of a Clostridial botulinum serotype A HA-33
1.alpha.-fold, a Clostridial botulinum serotype B HA-33
1.alpha.-fold, a Clostridial botulinum serotype C1 HA-33
1.alpha.-fold, a Clostridial botulinum serotype D HA-33
1.alpha.-fold, a Clostridial botulinum serotype A HA-33
2.alpha.-fold, a Clostridial botulinum serotype B HA-33
2.alpha.-fold, a Clostridial botulinum serotype C1 HA-33
2.alpha.-fold, a Clostridial botulinum serotype D HA-33
2.alpha.-fold, a Clostridial botulinum serotype A HA-17
.alpha.-fold, a Clostridial botulinum serotype B HA-17
.alpha.-fold, a Clostridial botulinum serotype C1 HA-17
.alpha.-fold, a Clostridial botulinum serotype D HA-17
.alpha.-fold, a Clostridial botulinum serotype A NTNH .alpha.-fold,
a Clostridial botulinum serotype B NTNH .alpha.-fold, a Clostridial
botulinum serotype C1 NTNH .alpha.-fold, a Clostridial botulinum
serotype D NTNH .alpha.-fold, a Clostridial botulinum serotype E
NTNH .alpha.-fold, a Clostridial botulinum serotype F NTNH
.alpha.-fold, a Clostridial botulinum serotype G NTNH .alpha.-fold,
a FGF-1.alpha.-fold, a FGF-2 .alpha.-fold, a FGF-4 .alpha.-fold, a
FGF-8 .alpha.-fold, a FGF-9 .alpha.-fold, a FGF-17 .alpha.-fold and
a FGF-18 .alpha.-fold. [0900] 111. The polynucleotide molecule
according to 101, wherein the polynucleotide molecule encoding a
modified Clostridial toxin H.sub.CC targeting domain comprises a
polynucleotide molecule encoding a substitution of a Clostridial
toxin H.sub.CC targeting domain .beta.-fold for a .beta.-fold
selected from the group consisting of a Clostridial botulinum
serotype A HA-33 1.beta.-fold, a Clostridial botulinum serotype B
HA-33 1.beta.-fold, a Clostridial botulinum serotype C1 HA-33
1.beta.-fold, a Clostridial botulinum serotype D HA-33
1.beta.-fold, a Clostridial botulinum serotype A HA-33
2.beta.-fold, a Clostridial botulinum serotype B HA-33
2.beta.-fold, a Clostridial botulinum serotype C1 HA-33
2.beta.-fold, a Clostridial botulinum serotype D HA-33
2.beta.-fold, a Clostridial botulinum serotype A HA-17
1.beta.-fold, a Clostridial botulinum serotype B HA-17
1.beta.-fold, a Clostridial botulinum serotype C1 HA-17
1.beta.-fold, a Clostridial botulinum serotype D HA-17
1.beta.-fold, a Clostridial botulinum serotype A NTNH .beta.-fold,
a Clostridial botulinum serotype B NTNH .beta.-fold, a Clostridial
botulinum serotype C1 NTNH .beta.-fold, a Clostridial botulinum
serotype D NTNH .beta.-fold, a Clostridial botulinum serotype E
NTNH .beta.-fold, a Clostridial botulinum serotype F NTNH
.beta.-fold, a Clostridial botulinum serotype G NTNH .beta.-fold, a
FGF-1 .beta.-fold, a FGF-2 .beta.-fold, a FGF-4 .beta.-fold, a
FGF-8 .beta.-fold, a FGF-9 .beta.-fold, a FGF-17 .beta.-fold and a
FGF-18 .beta.-fold. 112. The polynucleotide molecule according to
101, wherein the polynucleotide molecule encoding a modified
Clostridial toxin H.sub.CC targeting domain comprises a
polynucleotide molecule encoding a substitution of a Clostridial
toxin H.sub.CC targeting domain .gamma.-fold for a .gamma.-fold
selected from the group consisting of a Clostridial botulinum
serotype A HA-33 1.gamma.-fold, a Clostridial botulinum serotype B
HA-33 1.gamma.-fold, a Clostridial botulinum serotype C1 HA-33
1.gamma.-fold, a Clostridial botulinum serotype D HA-33
1.gamma.-fold, a Clostridial botulinum serotype A HA-33
2.gamma.-fold, a Clostridial botulinum serotype B HA-33
2.gamma.-fold, a Clostridial botulinum serotype C1 HA-33
2.gamma.-fold, a Clostridial botulinum serotype D HA-33
2.gamma.-fold, a Clostridial botulinum serotype A HA-17
.gamma.-fold, a Clostridial botulinum serotype B HA-17
.gamma.-fold, a Clostridial botulinum serotype C1 HA-17
.gamma.-fold, a Clostridial botulinum serotype D HA-17
.gamma.-fold, a Clostridial botulinum serotype A NTNH .gamma.-fold,
a Clostridial botulinum serotype B NTNH .gamma.-fold, a Clostridial
botulinum serotype C1 NTNH .gamma.-fold, a Clostridial botulinum
serotype D NTNH .gamma.-fold, a Clostridial botulinum serotype E
NTNH .gamma.-fold, a Clostridial botulinum serotype F NTNH
.gamma.-fold, a Clostridial botulinum serotype G NTNH .gamma.-fold,
a FGF-1 .gamma.-fold, a FGF-2 .gamma.-fold, a FGF-4 .gamma.-fold, a
FGF-8 .gamma.-fold, a FGF-9 .gamma.-fold, a FGF-17 .gamma.-fold and
a FGF-18 .gamma.-fold. [0901] 113. The polynucleotide molecule
according to 101, wherein the polynucleotide molecule encoding a
modified Clostridial toxin H.sub.CC targeting domain comprises a
polynucleotide molecule encoding a substitution of a Clostridial
toxin H.sub.CC targeting domain .beta.4/.beta.5 .beta.-hairpin turn
for a .beta.4/.beta.5 .beta.-hairpin turn selected from the group
consisting of a Clostridial botulinum serotype A HA-33
.beta.4/.beta.5 .beta.-hairpin turn, a Clostridial botulinum
serotype B HA-33 1.beta.4/.beta.5 .beta.-hairpin turn, a
Clostridial botulinum serotype C1 HA-33 1.beta.4/.beta.5
.beta.-hairpin turn, a Clostridial botulinum serotype D HA-33
1.beta.4/.beta.5 .beta.-hairpin turn, a Clostridial botulinum
serotype A HA-33 2.beta.4/.beta.5 .beta.-hairpin turn, a
Clostridial botulinum serotype B HA-33 2.beta.4/.beta.5
.beta.-hairpin turn, a Clostridial botulinum serotype C1 HA-33
2.beta.4/.beta.5 .beta.-hairpin turn, a Clostridial botulinum
serotype D HA-33 2.beta.4/.beta.5 .beta.-hairpin turn, a
Clostridial botulinum serotype A HA-17 .beta.4/.beta.5
.beta.-hairpin turn, a Clostridial botulinum serotype B HA-17
.beta.4/.beta.5 .beta.-hairpin turn, a Clostridial botulinum
serotype C1 HA-17 .beta.4/.beta.5 .beta.-hairpin turn, a
Clostridial botulinum serotype D HA-17 .beta.4/.beta.5
.beta.-hairpin turn, a Clostridial botulinum serotype A NTNH
.beta.4/.beta.5 .beta.-hairpin turn, a Clostridial botulinum
serotype B NTNH .beta.4/.beta.5 .beta.-hairpin turn, a Clostridial
botulinum serotype C1 NTNH .beta.4/.beta.5 .beta.-hairpin turn, a
Clostridial botulinum serotype D NTNH .beta.4/.beta.5
.beta.-hairpin turn, a Clostridial botulinum serotype E NTNH
.beta.4/.beta.5 .beta.-hairpin turn, a Clostridial botulinum
serotype F NTNH .beta.4/.beta.5 .beta.-hairpin turn, a Clostridial
botulinum serotype G NTNH .beta.4/.beta.5 .beta.-hairpin turn, a
FGF-1 .beta.4/.beta.5 .beta.-hairpin turn, a FGF-2 .beta.4/.beta.5
.beta.-hairpin turn, a FGF-4 .beta.4/.beta.5 .beta.-hairpin turn, a
FGF-8 .beta.4/.beta.5 .beta.-hairpin turn, a FGF-9 .beta.4/.beta.5
.beta.-hairpin turn, a FGF-17 .beta.4/.beta.5 .beta.-hairpin turn
and a FGF-18 .beta.4/.beta.5 .beta.-hairpin turn. [0902] 114. The
polynucleotide molecule according to 101, wherein the
polynucleotide molecule encoding a modified Clostridial toxin
H.sub.CC targeting domain comprises a polynucleotide molecule
encoding a substitution of a Clostridial toxin H.sub.CC targeting
domain .beta.8/.beta.9 .beta.-hairpin turn for a .beta.8/.beta.9
.beta.-hairpin turn selected from the group consisting of a
Clostridial botulinum serotype A HA-33 .beta.8/.beta.9
.beta.-hairpin turn, a Clostridial botulinum serotype B HA-33
1.beta.8/.beta.9 .beta.-hairpin turn, a Clostridial botulinum
serotype Cl HA-33 1.beta.8/.beta.9 .beta.-hairpin turn, a
Clostridial botulinum serotype D HA-33 1.beta.8/.beta.9
.beta.-hairpin turn, a Clostridial botulinum serotype A HA-33
2.beta.8/.beta.9 .beta.-hairpin turn, a Clostridial botulinum
serotype B HA-33 2.beta.8/.beta.9 .beta.-hairpin turn, a
Clostridial botulinum serotype C1 HA-33 2.beta.8/.beta.9
.beta.-hairpin turn, a Clostridial botulinum serotype D HA-33
2.beta.8/.beta.9 .beta.-hairpin turn, a Clostridial botulinum
serotype A HA-17 .beta.8/.beta.9 .beta.-hairpin turn, a Clostridial
botulinum serotype B HA-17 .beta.8/.beta.9 .beta.-hairpin turn, a
Clostridial botulinum serotype C1 HA-17 .beta.8/.beta.9
.beta.-hairpin turn, a Clostridial botulinum serotype D HA-17
.beta.8/.beta.9 .beta.-hairpin turn, a Clostridial botulinum
serotype A NTNH .beta.8/.beta.9 .beta.-hairpin turn, a Clostridial
botulinum serotype B NTNH .beta.8/.beta.9 .beta.-hairpin turn, a
Clostridial botulinum serotype C1 NTNH .beta.8/.beta.9
.beta.-hairpin turn, a Clostridial botulinum serotype D NTNH
.beta.8/.beta.9 .beta.-hairpin turn, a Clostridial botulinum
serotype E NTNH .beta.8/.beta.9 .beta.-hairpin turn, a Clostridial
botulinum serotype F NTNH .beta.8/.beta.9 .beta.-hairpin turn, a
Clostridial botulinum serotype G NTNH .beta.8/.beta.9
.beta.-hairpin turn, a FGF-1 .beta.8/.beta.9 .beta.-hairpin turn, a
FGF-2 .beta.8/.beta.9 .beta.-hairpin turn, a FGF-4 .beta.8/.beta.9
.beta.-hairpin turn, a FGF-8 .beta.8/.beta.9 .beta.-hairpin turn, a
FGF-9 .beta.8/.beta.9 .beta.-hairpin turn, a FGF-17
.beta.8/.beta.9 .beta.-hairpin turn and a FGF-18 .beta.8/.beta.9
.beta.-hairpin turn. [0903] 115. The polynucleotide molecule
according to 101, wherein the polynucleotide molecule encoding the
protease cleavage site is a polynucleotide molecule encoding an
endogenous Clostridial toxin di-chain loop protease cleavage site
or a polynucleotide molecule encoding an exogenous cleavage site.
[0904] 116. The polynucleotide molecule according to 101, wherein
the polynucleotide molecule comprises an expression vector. [0905]
117. A polynucleotide molecule encoding a modified Clostridial
toxin, the polynucleotide molecule comprising: [0906] a) a
polynucleotide molecule encoding a Clostridial toxin enzymatic
domain capable of executing an enzymatic target modification step
of a Clostridial toxin intoxication process; [0907] b) a
polynucleotide molecule encoding a Clostridial toxin translocation
domain capable of executing a translocation step of a Clostridial
toxin intoxication process; [0908] c) a polynucleotide molecule
encoding a translocation facilitating domain capable of
facilitating a translocation step of a Clostridial toxin
intoxication process; [0909] d) a polynucleotide molecule encoding
an enhanced targeting domain comprising a Clostridial non-toxin
associated protein .beta.-trefoil domain capable of executing a
cell binding step of a Clostridial toxin intoxication process; and
[0910] e) a polynucleotide molecule encoding a protease cleavage
site [0911] wherein cleavage of the protease cleavage site converts
the single-chain form of the modified Clostridial toxin into the
di-chain form. [0912] 118. The polynucleotide molecule according to
115, wherein the polynucleotide molecule encodes a modified
Clostridial toxin of any one of claims 45-51. [0913] 119. The
polynucleotide molecule according to 117, wherein the
polynucleotide molecule encoding a Clostridial toxin enzymatic
domain is selected from the group consisting of a polynucleotide
molecule encoding a BoNT/A enzymatic domain, a BoNT/B enzymatic
domain, a BoNT/C1 enzymatic domain, a BoNT/D enzymatic domain, a
BoNT/E enzymatic domain, a BoNT/F enzymatic domain, a BoNT/G
enzymatic domain and a TeNT enzymatic domain. [0914] 120. The
polynucleotide molecule according to 117, wherein the
polynucleotide molecule encoding a Clostridial toxin translocation
domain is selected from the group consisting of a polynucleotide
molecule encoding a BoNT/A translocation domain, a BoNT/B
translocation domain, a BoNT/C1 translocation domain, a BoNT/D
translocation domain, a BoNT/E translocation domain, a BoNT/F
translocation domain, a BoNT/G translocation domain and a TeNT
translocation domain. [0915] 121. The polynucleotide molecule
according to 117, wherein the translocation facilitating domain is
a Clostridial toxin translocation facilitating domain. [0916] 122.
The polynucleotide molecule according to 121, wherein the
Clostridial toxin translocation facilitating domain is selected
from the group consisting of a BoNT/A translocation facilitating
domain, a BoNT/B translocation facilitating domain, a BoNT/C1
translocation facilitating domain, a BoNT/D translocation domain, a
BoNT/E translocation facilitating domain, a BoNT/F translocation
facilitating domain, a BoNT/G translocation facilitating domain and
a TeNT translocation facilitating domain. [0917] 123. The
polynucleotide molecule according to 117, wherein the translocation
facilitating domain is an enveloped virus fusogenic peptide domain.
[0918] 124. The polynucleotide molecule according to 123, wherein
the enveloped virus fusogenic peptide domain is selected from the
group consisting of an influenzavirus fusogenic peptide domain, an
alphavirus fusogenic peptide domain, a vesiculovirus fusogenic
peptide domain, a respirovirus fusogenic peptide domain, a
morbillivirus fusogenic peptide domain, an avulavirus fusogenic
peptide domain, a henipavirus fusogenic peptide domain, a
metapneumovirus fusogenic peptide domain and a foamy virus
fusogenic peptide domain. [0919] 125. The polynucleotide molecule
according to 117, wherein the polynucleotide molecule encoding a
Clostridial non-toxin associated protein .beta.-trefoil domain
selected from the group consisting of a polynucleotide molecule
encoding a Clostridial botulinum serotype A HA-33 .beta.-trefoil
domain, a Clostridial botulinum serotype B HA-33 .beta.-trefoil
domain, a Clostridial botulinum serotype C1 HA-33 .beta.-trefoil
domain, a Clostridial botulinum serotype D HA-33 .beta.-trefoil
domain, a Clostridial botulinum serotype A HA-17 .beta.-trefoil
domain, a Clostridial botulinum serotype B HA-17 .beta.-trefoil
domain, a Clostridial botulinum serotype C1 HA-17 .beta.-trefoil
domain, a Clostridial botulinum serotype D HA-17 .beta.-trefoil
domain, a Clostridial botulinum serotype A NTNH .beta.-trefoil
domain, a Clostridial botulinum serotype B NTNH .beta.-trefoil
domain, a Clostridial botulinum serotype C1 NTNH .beta.-trefoil
domain, a Clostridial botulinum serotype D NTNH .beta.-trefoil
domain, a Clostridial botulinum serotype E NTNH .beta.-trefoil
domain, a Clostridial botulinum serotype F NTNH .beta.-trefoil
domain and a Clostridial botulinum serotype G NTNH .beta.-trefoil
domain. [0920] 126. The polynucleotide molecule according to 117,
wherein the polynucleotide molecule encoding the protease cleavage
site is a polynucleotide molecule encoding an endogenous
Clostridial toxin di-chain loop protease cleavage site or a
polynucleotide molecule encoding an exogenous cleavage site. [0921]
127. The polynucleotide molecule according to 117, wherein the
polynucleotide molecule comprises an expression vector. [0922] 128.
A polynucleotide molecule encoding a modified Clostridial toxin,
the polynucleotide molecule comprising: [0923] a) a polynucleotide
molecule encoding a Clostridial toxin enzymatic domain capable of
executing an enzymatic target modification step of a Clostridial
toxin intoxication process; [0924] b) a polynucleotide molecule
encoding a Clostridial toxin translocation domain capable of
executing a translocation step of a Clostridial toxin intoxication
process; [0925] c) a polynucleotide molecule encoding a Clostridial
toxin translocation facilitating domain capable of facilitating a
translocation step of a Clostridial toxin intoxication process;
[0926] d) a polynucleotide molecule encoding an enhanced targeting
domain comprising a FGF .beta.-trefoil domain capable of
selectively binding an FGFR3; and [0927] e) a polynucleotide
molecule encoding a protease cleavage site [0928] wherein cleavage
of the protease cleavage site converts the single-chain form of the
modified Clostridial toxin into the di-chain form. [0929] 129. The
polynucleotide molecule according to 128, wherein the
polynucleotide molecule encodes a modified Clostridial toxin of any
one of claims 73-79. [0930] 130. The polynucleotide molecule
according to 128, wherein the polynucleotide molecule encoding a
Clostridial toxin enzymatic domain is selected from the group
consisting of a polynucleotide molecule encoding a BoNT/A enzymatic
domain, a BoNT/B enzymatic domain, a BoNT/C1 enzymatic domain, a
BoNT/D enzymatic domain, a BoNT/E enzymatic domain, a BoNT/F
enzymatic domain, a BoNT/G enzymatic domain and a TeNT enzymatic
domain. [0931] 131. The polynucleotide molecule according to 128,
wherein the polynucleotide molecule encoding a Clostridial toxin
translocation domain is selected from the group consisting of a
polynucleotide molecule encoding a BoNT/A translocation domain, a
BoNT/B translocation domain, a BoNT/C1 translocation domain, a
BoNT/D translocation domain, a BoNT/E translocation domain, a
BoNT/F translocation domain, a BoNT/G translocation domain and a
TeNT translocation domain. [0932] 132. The polynucleotide molecule
according to 128, wherein the translocation facilitating domain is
a Clostridial toxin translocation facilitating domain. [0933] 133.
The polynucleotide molecule according to 132, wherein the
Clostridial toxin translocation facilitating domain is selected
from the group consisting of a BoNT/A translocation facilitating
domain, a BoNT/B translocation facilitating domain, a BoNT/C1
translocation facilitating domain, a BoNT/D translocation domain, a
BoNT/E translocation facilitating domain, a BoNT/F translocation
facilitating domain, a BoNT/G translocation facilitating domain and
a TeNT translocation facilitating domain. [0934] 134. The
polynucleotide molecule according to 128, wherein the translocation
facilitating domain is an enveloped virus fusogenic peptide domain.
[0935] 135. The polynucleotide molecule according to 134, wherein
the enveloped virus fusogenic peptide domain is selected from the
group consisting of an influenzavirus fusogenic peptide domain, an
alphavirus fusogenic peptide domain, a vesiculovirus fusogenic
peptide domain, a respirovirus fusogenic peptide domain, a
morbillivirus fusogenic peptide domain, an avulavirus fusogenic
peptide domain, a henipavirus fusogenic peptide domain, a
metapneumovirus fusogenic peptide domain and a foamy virus
fusogenic peptide domain. [0936] 136. The polynucleotide molecule
according to 128, wherein the polynucleotide molecule encoding a
FGF .beta.-trefoil domain is selected from the group consisting of
a polynucleotide molecule encoding a FGF-1 .beta.-trefoil domain, a
FGF-2 .beta.-trefoil domain, a FGF-4 .beta.-trefoil domain, a FGF-8
.beta.-trefoil domain, a FGF-9 .beta.-trefoil domain, a FGF-17
.beta.-trefoil domain and a FGF-18 .beta.-trefoil domain. [0937]
137. The polynucleotide molecule according to 128, wherein the
polynucleotide molecule encoding the protease cleavage site is a
polynucleotide molecule encoding an endogenous Clostridial toxin
di-chain loop protease cleavage site or a polynucleotide molecule
encoding an exogenous cleavage site. [0938] 138. The polynucleotide
molecule according to 128, wherein the polynucleotide molecule
comprises an expression vector. [0939] 139. A method of producing a
modified Clostridial toxin comprising the step of expressing a
polynucleotide molecule encoding a modified Clostridial toxin in a
cell, the polynucleotide molecule comprising: [0940] a) a
polynucleotide molecule encoding a Clostridial toxin enzymatic
domain capable of executing an enzymatic target modification step
of a Clostridial toxin intoxication process; [0941] b) a
polynucleotide molecule encoding a Clostridial toxin translocation
domain capable of executing a translocation step of a Clostridial
toxin intoxication process; [0942] c) a polynucleotide molecule
encoding a translocation facilitating domain capable of
facilitating a translocation step of a Clostridial toxin
intoxication process; [0943] d) a polynucleotide molecule encoding
an enhanced targeting domain comprising a modified Clostridial
H.sub.CC targeting domain capable of executing a cell binding step
of a Clostridial toxin intoxication process; and [0944] e) a
polynucleotide molecule encoding a protease cleavage site [0945]
wherein cleavage of the protease cleavage site converts the
single-chain form of the modified Clostridial toxin into the
di-chain form; and [0946] wherein the modified Clostridial H.sub.CC
targeting domain exhibits enhanced binding activity for an
endogenous Clostridial toxin receptor relative to the binding
activity of a naturally-occurring Clostridial H.sub.CC targeting
domain from which the modified Clostridial binding H.sub.CC
targeting is derived. [0947] 140. The method according to 139,
wherein the polynucleotide molecule is any one of the
polynucleotide molecules of 102. [0948] 141. A method of producing
a modified Clostridial toxin comprising the step of expressing a
polynucleotide molecule encoding a modified Clostridial toxin in a
cell, the polynucleotide molecule comprising: [0949] a) a
polynucleotide molecule encoding a Clostridial toxin enzymatic
domain capable of executing an enzymatic target modification step
of a Clostridial toxin intoxication process; [0950] b) a
polynucleotide molecule encoding a Clostridial toxin translocation
domain capable of executing a translocation step of a Clostridial
toxin intoxication process; [0951] c) a polynucleotide molecule
encoding a translocation facilitating domain capable of
facilitating a translocation step of a Clostridial toxin
intoxication process; [0952] d) a polynucleotide molecule encoding
an enhanced targeting domain comprising a Clostridial non-toxin
associated protein .beta.-trefoil domain capable of executing a
cell binding step of a Clostridial toxin intoxication process; and
[0953] e) a polynucleotide molecule encoding a protease cleavage
site [0954] wherein cleavage of the protease cleavage site converts
the single-chain form of the modified Clostridial toxin into the
di-chain form. [0955] 142. The method according to 141, wherein the
polynucleotide molecule is any one of the polynucleotide molecules
of 118. [0956] 143. A method of producing a modified Clostridial
toxin comprising the step of expressing a polynucleotide molecule
encoding a modified Clostridial toxin in a cell, the polynucleotide
molecule comprising: [0957] a) a polynucleotide molecule encoding a
Clostridial toxin enzymatic domain capable of executing an
enzymatic target modification step of a Clostridial toxin
intoxication process; [0958] b) a polynucleotide molecule encoding
a Clostridial toxin translocation domain capable of executing a
translocation step of a Clostridial toxin intoxication process;
[0959] c) a polynucleotide molecule encoding a translocation
facilitating domain capable of facilitating a translocation step of
a Clostridial toxin intoxication process; [0960] d) a
polynucleotide molecule encoding an enhanced targeting domain
comprising a FGF .beta.-trefoil domain capable of selectively
binding an FGFR3; and [0961] e) a polynucleotide molecule encoding
a protease cleavage site [0962] wherein cleavage of the protease
cleavage site converts the single-chain form of the modified
Clostridial toxin into the di-chain form. [0963] 144. The method
according to 143, wherein the polynucleotide molecule is any one of
the polynucleotide molecules of 129. [0964] 145. A method of
producing a modified Clostridial toxin comprising the steps of:
[0965] a) introducing into a cell a polynucleotide molecule
encoding a modified Clostridial toxin, the polynucleotide molecule
comprising: [0966] i) a polynucleotide molecule encoding a
Clostridial toxin enzymatic domain capable of executing an
enzymatic target modification step of a Clostridial toxin
intoxication process; [0967] ii) a polynucleotide molecule encoding
a Clostridial toxin translocation domain capable of executing a
translocation step of a Clostridial toxin intoxication process;
[0968] iii) a polynucleotide molecule encoding a translocation
facilitating domain capable of facilitating a translocation step of
a Clostridial toxin intoxication process; [0969] iv) a
polynucleotide molecule encoding an enhanced targeting domain
comprising a modified Clostridial H
.sub.CC targeting domain capable of executing a cell binding step
of a Clostridial toxin intoxication process; and [0970] v) a
polynucleotide molecule encoding a protease cleavage site [0971]
wherein cleavage of the protease cleavage site converts the
single-chain form of the modified Clostridial toxin into the
di-chain form; and [0972] wherein the modified Clostridial H.sub.CC
targeting domain exhibits enhanced binding activity for an
endogenous Clostridial toxin receptor relative to the binding
activity of a naturally-occurring Clostridial H.sub.CC targeting
domain from which the modified Clostridial H.sub.CC targeting
domain is derived. [0973] b) expressing the modified Clostridial
toxin encoded by the polynucleotide molecule. [0974] 146. The
method according to 145, wherein the polynucleotide molecule is any
one of the polynucleotide molecules of 102. [0975] 147. A method of
producing a modified Clostridial toxin comprising the steps of:
[0976] a) introducing into a cell a polynucleotide molecule
encoding a modified Clostridial toxin, the polynucleotide molecule
comprising: [0977] i) a polynucleotide molecule encoding a
Clostridial toxin enzymatic domain capable of executing an
enzymatic target modification step of a Clostridial toxin
intoxication process; [0978] ii) a polynucleotide molecule encoding
a Clostridial toxin translocation domain capable of executing a
translocation step of a Clostridial toxin intoxication process;
[0979] iii) a polynucleotide molecule encoding a translocation
facilitating domain capable of facilitating a translocation step of
a Clostridial toxin intoxication process; [0980] iv) a
polynucleotide molecule encoding an enhanced targeting domain
comprising a Clostridial non-toxin associated protein
.beta.-trefoil domain capable of executing a cell binding step of a
Clostridial toxin intoxication process; and [0981] v) a
polynucleotide molecule encoding a protease cleavage site [0982]
wherein cleavage of the protease cleavage site converts the
single-chain form of the modified Clostridial toxin into the
di-chain form. [0983] b) expressing the modified Clostridial toxin
encoded by the polynucleotide molecule. [0984] 148. The method
according to 147, wherein the polynucleotide molecule is any one of
the polynucleotide molecules of 118. [0985] 149. A method of
producing a modified Clostridial toxin comprising the steps of:
[0986] a) introducing into a cell a polynucleotide molecule
encoding a modified Clostridial toxin, the polynucleotide molecule
comprising: [0987] i) a polynucleotide molecule encoding a
Clostridial toxin enzymatic domain capable of executing an
enzymatic target modification step of a Clostridial toxin
intoxication process; [0988] ii) a polynucleotide molecule encoding
a Clostridial toxin translocation domain capable of executing a
translocation step of a Clostridial toxin intoxication process;
[0989] iii) a polynucleotide molecule encoding a translocation
facilitating domain capable of facilitating a translocation step of
a Clostridial toxin intoxication process; [0990] iv) a
polynucleotide molecule encoding an enhanced targeting domain
comprising a FGF .beta.-trefoil domain capable of selectively
binding an FGFR3; and [0991] v) a polynucleotide molecule encoding
a protease cleavage site [0992] wherein cleavage of the protease
cleavage site converts the single-chain form of the modified
Clostridial toxin into the di-chain form. [0993] b) expressing the
modified Clostridial toxin encoded by the polynucleotide molecule.
[0994] 150. The method according to 149, wherein the polynucleotide
molecule is any one of the polynucleotide molecules of 129.
EXAMPLES
[0995] The following non-limiting examples are provided for
illustrative purposes only in order to facilitate a more complete
understanding of disclosed embodiments and are in no way intended
to limit any of the embodiments disclosed in the present
specification.
Example 1
Construction of a Modified Clostridial Toxin Comprising a
Translocation Facilitating Domain and a Modified Clostridial Toxin
Targeting Domain with Enhanced Binding Activity
[0996] This example illustrates how to make a modified Clostridial
toxin comprising a translocation facilitating domain and a modified
Clostridial toxin binding domain with enhanced binding activity
using site-directed mutagenesis.
[0997] A polynucleotide molecule encoding BoNT/A (SEQ ID NO: 1) is
synthesized using standard procedures (BlueHeron.RTM.
Biotechnology, Bothell, Wash.). Oligonucleotides of 20 to 50 bases
in length are synthesized using standard phosphoramidite synthesis.
These oligonucleotides are hybridized into double stranded duplexes
that are ligated together to assemble the full-length
polynucleotide molecule. This polynucleotide molecule is cloned
using standard molecular biology methods into a pUCBHB1 vector at
the SmaI site to generate pUCBHB1/BoNT/A. The synthesized
polynucleotide molecule is verified by sequencing using Big Dye
Terminator.TM. Chemistry 3.1 (Applied Biosystems, Foster City,
Calif.) and an ABI 3100 sequencer (Applied Biosystems, Foster City,
Calif.).
[0998] If desired, an expression optimized polynucleotide molecule
encoding BoNT/A (SEQ ID NO: 1) can be synthesized in order to
improve expression in an Escherichia coli strain. The
polynucleotide molecule encoding the BoNT/A can be modified to 1)
contain synonymous codons typically present in native
polynucleotide molecules of an Escherichia coli strain; 2) contain
a G+C content that more closely matches the average G+C content of
native polynucleotide molecules found in an Escherichia coli
strain; 3) reduce polymononucleotide regions found within the
polynucleotide molecule; and/or 4) eliminate internal regulatory or
structural sites found within the polynucleotide molecule, see,
e.g., Lance E. Steward et al. Optimizing Expression of Active
Botulinum Toxin Type E, PCT Patent Serial No. 2005/020578 (Jun. 9,
2005); Lance E. Steward et al. Optimizing Expression of Active
Botulinum Toxin Type A, PCT Patent Serial No. 2005/027917 (Aug. 3,
2005). Once sequence optimization is complete, oligonucleotides of
20 to 50 bases in length are synthesized using standard
phosphoramidite synthesis. These oligonucleotides are hybridized
into double stranded duplexes that are ligated together to assemble
the full-length polynucleotide molecule. This polynucleotide
molecule is cloned using standard molecular biology methods into a
pUCBHB1 vector at the SmaI site to generate pUCBHB1/BoNT/A. The
synthesized polynucleotide molecule is verified by sequencing using
Big Dye Terminator.TM. Chemistry 3.1 (Applied Biosystems, Foster
City, Calif.) and an ABI 3100 sequencer (Applied Biosystems, Foster
City, Calif.). Is so desired, optimization to a different organism,
such as, e.g., a yeast strain, an insect cell-line or a mammalian
cell line, can be done, see, e.g., Steward, supra, PCT Patent
Serial No. 2005/020578 (Jun. 9, 2005); and Steward, supra, PCT
Patent Serial No. 2005/027917 (Aug. 3, 2005).
[0999] A similar cloning strategy is used to make pUCBHB1 cloning
constructs comprising a polynucleotide molecule encoding BoNT/B of
SEQ ID NO: 2; a polynucleotide molecule encoding BoNT/C1 of SEQ ID
NO: 3; a polynucleotide molecule encoding BoNT/D of SEQ ID NO: 4; a
polynucleotide molecule encoding BoNT/E of SEQ ID NO: 5; a
polynucleotide molecule encoding BoNT/F of SEQ ID NO: 6; a
polynucleotide molecule encoding BoNT/G of SEQ ID NO: 7; and a
polynucleotide molecule encoding TeNT of SEQ ID NO: 8. In addition,
one skilled in the art can modify Clostridial toxins, such as,
e.g., to include an exogenous protease cleavage site within the
di-chain loop region, or flexible spacer regions.
[1000] To construct pET29/BoNT/A, a pUCBHB1/BoNT/A construct is
digested with restriction endonucleases that 1) excise the insert
comprising the open reading frame of SEQ ID NO: 1 encoding BoNT/A;
and 2) enable this insert to be operably-linked to a pET29 vector
(EMD Biosciences-Novagen, Madison, Wis.). This insert is subcloned
using a T4 DNA ligase procedure into a pET29 vector that is
digested with appropriate restriction endonucleases to yield
pET29/BoNT/A. The ligation mixture is transformed into chemically
competent E. coli DH5.alpha. cells (Invitrogen, Inc, Carlsbad,
Calif.) using a heat shock method, plated on 1.5% Luria-Bertani
agar plates (pH 7.0) containing 50 .mu.g/mL of Kanamycin, and
placed in a 37.degree. C. incubator for overnight growth. Bacteria
containing expression constructs are identified as Kanamycin
resistant colonies. Candidate constructs are isolated using an
alkaline lysis plasmid mini-preparation procedure and analyzed by
restriction endonuclease digest mapping to determine the presence
and orientation of the insert. This cloning strategy yielded a
pET29 expression construct comprising the polynucleotide molecule
encoding the BoNT/A of SEQ ID NO: 1 operably-linked to a carboxyl
terminal polyhistidine affinity binding peptide.
[1001] A similar cloning strategy is used to make pET29 expression
constructs comprising a polynucleotide molecule encoding BoNT/B of
SEQ ID NO: 2; a polynucleotide molecule encoding BoNT/C1 of SEQ ID
NO: 3; a polynucleotide molecule encoding BoNT/D of SEQ ID NO: 4; a
polynucleotide molecule encoding BoNT/E of SEQ ID NO: 5; a
polynucleotide molecule encoding BoNT/F of SEQ ID NO: 6; a
polynucleotide molecule encoding BoNT/G of SEQ ID NO: 7; and a
polynucleotide molecule encoding TeNT of SEQ ID NO: 8.
[1002] To construct a modified BoNT/A comprising a modified
H.sub.CC targeting domain with enhanced binding activity, specific
amino acids influencing binding activity will be changed. It is
already known that amino acids Trp 1101, Gly 1102, Leu 1105, Tyr
1111, Tyr 1112, Gly 1158, Ile 1163, Asp 1179, Glu 1203, Phe 1252,
Ser 1264, Trp 1266, Tyr 1267, Gln 1270, Gly 1279 and Trp 1282 of
SEQ ID NO: 1 are important for function. To determine which amino
acid substitutions could enhance the binding activity of a BoNT/A
binding domain, computational protein design algorithms will
generate novel proteins with optimized properties. The crystal
structure of a BoNT/A binding domain will be used as the starting
template for computational calculations. Potential amino acid
candidates will be identified using a combined output from Protein
Design Automation.RTM. (PDA.RTM.) and Sequence Prediction
Algorithm.TM. (SPA.TM.) calculations. For PDA calculations, the
conformations of amino acids at variable positions will be
represented as a set of backbone-independent side chain rotamers
derived from the rotamer library. The energies of all possible
combinations of the considered amino acids at the chosen variable
positions will be calculated using a force field containing terms
describing van der Waals, solvation, electrostatic, and hydrogen
bond interactions. The optimal (ground state) sequence will be
determined using a Dead End Elimination (DEE) algorithm, and a
Monte Carlo (MC) algorithm will be used to evaluate the energies of
similar sequences around the predicted ground state. SPA
calculations utilize a genetic algorithm to screen for low energy
sequences, with energies being calculated during each round of
"evolution" for those sequences being sampled. The conformations of
amino acids will be represented as a set of side chain rotamers
derived from a backbone-independent rotamer library using a
flexible rotamer model. SPA calculations will generate sequences
which will be subsequently clustered computationally into groups of
similar sequences using a nearest neighbor single linkage
hierarchical clustering algorithm. Critical contact amino acids
will be fixed in both sequence and conformation and calculations
will be carried out to evaluate single and combinatorial
substitutions at variable amino acids. All amino acids in contact
with these residues will be floated, that is the amino acid
conformation but not the amino acid identity will be allowed to
vary to allow for conformational adjustments. Final experimental
substitutions will be chosen based on their predicted energies
relative to the naturally occurring BoNT/A binding domain and their
occupancy, that is the number times the substitution occurred in
the set of 1000 MC or genetic algorithm sequences. Two sets of
design calculations will be carried out using Rosetta to identify
substitutions predicted to stabilize the BoNT/A binding domain. In
the first round, only single amino acid substitutions will be
modeled. In a second round, interface amino acids will be allowed
to change to all 20 naturally occurring amino acids including the
native amino acid type, but excluding cysteine, simultaneously. In
each case, amino acid side chains contacting the substituted amino
acid side chains will be repacked (allowing all rotamers of the
native amino acid type). Sequences and conformations with low
energies will be selected using a Monte-Carlo simulated annealing
procedure. All resulting protein complex models will be rescored by
computing a predicted binding energy. Final sequences were selected
for the lowest binding energy
[1003] Identified candidate amino acids will be changed using
site-directed in vitro mutagenesis. A 50 .mu.L reaction will be
assembled using pET29/BoNT/A as a template, sense and antisense
oligonucleotides encoding the desired amino acid change identified
above, and reagents included with the QuickChange.RTM. II XL
Site-Directed Mutagenesis kit (Stratagene, La Jolla, Calif.). The
polymerase chain reaction (PCR) mix will contain 5 .mu.L of
10.times. Buffer, 1 .mu.L of deoxyribonucleotides (dNTPs), 1 .mu.L
of PfuUltra.TM. High Fidelity DNA polymerase (2.5 units/.mu.L), 125
ng of each primer, 100 ng of template DNA, and nuclease-free water
to a final volume of 50 .mu.L. The thermocycler conditions will be:
one cycle of 95.degree. C. for 60 seconds; 16 cycles of 95.degree.
C. for 30 seconds, 55.degree. C. for 60 seconds, and 72.degree. C.
for 10 minutes; one cycle of 72.degree. C. for 5 minutes; and
4.degree. C. to hold. Following thermocycling, 1 .mu.L of Dpnl
restriction enzyme (Stratagene, La Jolla, Calif.) will be added to
the reaction and will be incubated for 1 hour at 37.degree. C. to
digest the template DNA. The reaction will be purified by QIAquick
kit (QIAGEN, Inc., Valencia, Calif.) and will be analysis by
agarose gel electrophoresis to determine that the reaction produced
full-length plasmid. The mutagenesis products will be transformed
into chemically competent E. coli DH5.alpha. cells (Invitrogen,
Inc, Carlsbad, Calif.) using a heat shock method, plated on 1.5%
Luria-Bertani agar plates (pH 7.0) containing 100 .mu.g/mL of
Ampicillin, and will be placed in a 37.degree. C. incubator for
overnight growth. Candidate mutagenesis constructs will be isolated
as Ampicillin resistant colonies and will be analyzed using an
alkaline lysis plasmid mini-preparation procedure to isolate the
expression construct and restriction endonuclease digests to
determine the presence of the insert. The incorporation of the
point mutation will be determined by sequence analysis of candidate
plasmid constructs.
[1004] To test the binding activity of modified BoNT/A candidates
with enhanced binding activity, the soluble portion of FGFR3 will
be expressed recombinantly for use in surface plasmon resonance
(SPR) binding assays, e.g., Biacore.RTM. (Biacore Inc., Piscataway,
N.J.). The soluble portion of FGFR3 will be expressed as a fusion
to streptavidin and the receptor will then be immobilized on an
appropriate sensor chip. Utilizing a Biacore.RTM. instrument,
changes in local refractive index as a result of receptor binding
will be measured as a change in the SPR angle. The rates of change
in the SPR angle will then be analyzed to determine association
rate (K.sub.on), dissociation rate (K.sub.off) and the dissociation
equilibrium constant (K.sub.D=K.sub.off/K.sub.on). Modified BoNT/A
candidates with enhanced binding activity will exhibit either an
increased association rate, a decreased dissociation rate, both an
increased association rate and a decreased dissociation rate, or a
decreased dissociation equilibrium constant relative to the
measurements obtained from the naturally occurring BoNT/A from
which the modified BoNT/A with enhanced binding activity was
derived.
[1005] To construct a modified BoNT/A described above that replaces
the BoNT/A translocation facilitating domain with another
Clostridial toxin translocation facilitating domain, a
translocation facilitating domain of BoNT/B will be introduced into
the modified BoNT/A described above using a Splicing by Overlapping
ends polymerase chain reaction (SOE-PCR) procedure, see, e.g., R.
M. Horton et al., Engineering hybrid genes without the use of
restriction enzymes: gene splicing by overlapping extension, 77(1)
Gene 61-68 (1989); and R. M. Horton, PCR-mediated recombination and
mutagenesis. SOEing together tailor-made genes, 3(2) Mol.
Biotechnol. 93-99 (1995). A nucleic acid fragment comprising a
region encoding amino acids 859 to 1097 of BoNT/B (SEQ ID NO: 2)
will be operably-linked by SOE-PCR to replace the region
corresponding to the BoNT/A translocation facilitating domain of
amino acids 874-1110 of SEQ ID NO: 1 of the modified BoNT/A
described above and will be subcloned into a pCR2.1 vector using
the TOPO.RTM. TA cloning method (Invitrogen, Inc, Carlsbad,
Calif.). The forward and reverse oligonucleotide primers used for
these reactions are designed to include unique restriction enzyme
sites useful for subsequent subcloning steps. The resulting
construct is digested with restriction enzymes that 1) excise the
insert containing the entire open reading frame encoding the
modified BoNT/A; and 2) enable this insert to be operably-linked to
a pQBI-25A vector (Qbiogene, Inc., Carlsbad, Calif.). The resulting
restriction fragment will be purified by the QIAquick Gel
Extraction Kit (QIAGEN, Inc., Valencia, Calif.), and will be
subcloned using a T4 DNA ligase procedure into a pQBI-25A vector
(Qbiogene, Inc., Irvine, Calif.). This cloning strategy yielded a
pQBI-25 expression construct encoding a modified BoNT/A comprising
a BoNT/B translocation facilitating domain and a modified H.sub.CC
targeting domain with enhanced binding activity.
[1006] A similar cloning strategy is used to make pQBI-25
expression constructs comprising a polynucleotide molecule encoding
a modified BoNT/A comprising a modified targeting domain with
enhanced binding activity that includes, e.g., a translocation
facilitating domain comprising amino acids 869-1111 of BoNT/C1 of
SEQ ID NO: 3; a translocation facilitating domain comprising amino
acids 865-1098 of BoNT/D of SEQ ID NO: 4; a translocation
facilitating domain comprising amino acids 846-1058 of BoNT/E of
SEQ ID NO: 5; a translocation facilitating domain comprising amino
acids 867-1105 of BoNT/F of SEQ ID NO: 6; a translocation
facilitating domain comprising amino acids 866-1105 of BoNT/G of
SEQ ID NO: 7; or a translocation facilitating domain comprising
amino acids 882-1127 of TeNT of SEQ ID NO: 8.
Example 2
Construction of a Modified Clostridial Toxin Comprising a
Translocation Facilitating Domain and a Non-Toxin Associated
Protein
[1007] The following example illustrates how to make a modified
Clostridial toxin comprising a translocation facilitating domain
and an enhanced targeting domain comprising a non-toxin associated
protein.
[1008] A polynucleotide molecule encoding BoNT/A-33/A is
synthesized using standard procedures (BlueHeron.RTM.
Biotechnology, Bothell, Wash.), as described in Example 1.
BoNT/A-33/A is a BoNT/A modified to replace amino acids 1111-1296
of SEQ ID NO: 1, a BoNT/A .beta.-trefoil domain, with amino acids
151 to 293 of SEQ ID NO: 9, a HA-33 .beta.-trefoil domain from a
Clostridial botulinum serotype A strain. If desired, an expression
optimized polynucleotide molecule encoding BoNT/A-33/A can be
synthesized in order to improve expression in to a different
organism, such as, e.g., an Escherichia coli strain, a yeast
strain, an insect cell-line or a mammalian cell line, can be done,
see, e.g., Steward, supra, PCT Patent Serial No. 2005/020578 (Jun.
9, 2005); and Steward, supra, PCT Patent Serial No. 2005/027917
(Aug. 3, 2005). The synthesized polynucleotide molecule is verified
by sequencing using Big Dye Terminator.TM. Chemistry 3.1 (Applied
Biosystems, Foster City, Calif.) and an ABI 3100 sequencer (Applied
Biosystems, Foster City, Calif.).
[1009] A similar cloning strategy is used to make pUCBHB1 cloning
constructs for BoNT/B-33/A, a modified BoNT/B where amino acids
1098-1291 of SEQ ID NO: 2 are replaced with amino acids 151 to 293
of SEQ ID NO: 9; BoNT/C1-33/A, a modified BoNT/C1 where amino acids
1112-1291 of SEQ ID NO: 3 are replaced with amino acids 151 to 293
of SEQ ID NO: 9; BoNT/D-33/A, a modified BoNT/D where amino acids
1099-1276 of SEQ ID NO: 4 are replaced with amino acids 151 to 293
of SEQ ID NO: 9; BoNT/E-33/A, a modified BoNT/E where amino acids
1086-1252 of SEQ ID NO: 5 are replaced with amino acids 151 to 293
of SEQ ID NO: 9; BoNT/F-33/A, a modified BoNT/F where amino acids
1106-1274 of SEQ ID NO: 6 are replaced with amino acids 151 to 293
of SEQ ID NO: 9; BoNT/G-33/A, a modified BoNT/G where amino acids
1106-1297 of SEQ ID NO: 7 are replaced with amino acids 151 to 293
of SEQ ID NO: 9; and TeNT-33/A, a modified TeNT where amino acids
1128-1315 of SEQ ID NO: 8 are replaced with amino acids 151 to 293
of SEQ ID NO: 9. Similarly, the .beta.-trefoil domain from a
Clostridial toxin indicated above can be replaced with a non-toxin
associated protein .beta.-trefoil domain comprising amino acids
10-144 of SEQ ID NO: 9; amino acids 10-144 of SEQ ID NO: 10; amino
acids 10-144 of SEQ ID NO: 11; amino acids 10-146 of SEQ ID NO: 12;
amino acids 10-144 of SEQ ID NO: 13; amino acids 10-144 of SEQ ID
NO: 14; amino acids 10-146 of SEQ ID NO: 15; amino acids 10-141 of
SEQ ID NO: 16; amino acids 10-141 of SEQ ID NO: 17; amino acids
10-141 of SEQ ID NO: 18; amino acids 151-293 of SEQ ID NO: 10;
amino acids 151-293 of SEQ ID NO: 11; amino acids 153-294 of SEQ ID
NO: 12; amino acids 151-279 of SEQ ID NO: 13; amino acids 151-292
of SEQ ID NO: 14; amino acids 153-291 of SEQ ID NO: 15; amino acids
148-285 of SEQ ID NO: 16; amino acids 148-286 of SEQ ID NO: 17;
amino acids 148-286 of SEQ ID NO: 18; amino acids 9-146 of SEQ ID
NO: 19; amino acids 9-146 of SEQ ID NO: 20; amino acids 9-146 of
SEQ ID NO: 21; amino acids 9-146 of SEQ ID NO: 22; amino acids
1050-1194 of SEQ ID NO: 23; amino acids 1050-1199 of SEQ ID NO: 24;
amino acids 1050-1194 of SEQ ID NO: 25; amino acids 1049-1198 of
SEQ ID NO: 26; amino acids 1049-1197 of SEQ ID NO: 27; amino acids
1049-1197 of SEQ ID NO: 28; amino acids 1014-1163 of SEQ ID NO: 29;
amino acids 1016-1160 of SEQ ID NO: 30; amino acids 1017-1166 of
SEQ ID NO: 31; and amino acids 1050-1199 of SEQ ID NO: 32.
[1010] To construct pET29/BoNT/A-33/A, a pUCBHB1/BoNT/A-33/A
construct is digested with restriction endonucleases that 1) excise
the insert comprising the open reading frame encoding BoNT/A-33/A;
and 2) enable this insert to be operably-linked to a pET29 vector
(EMD Biosciences-Novagen, Madison, Wis.). This insert is subcloned
using a T4 DNA ligase procedure into a pET29 vector that is
digested with appropriate restriction endonucleases to yield
pET29/BoNT/A-33/A. The ligation mixture is transformed into
chemically competent E. coli DH5.alpha. cells (Invitrogen, Inc,
Carlsbad, Calif.) using a heat shock method, plated on 1.5%
Luria-Bertani agar plates (pH 7.0) containing 50 .mu.g/mL of
Kanamycin, and placed in a 37.degree. C. incubator for overnight
growth. Bacteria containing expression constructs are identified as
Kanamycin resistant colonies. Candidate constructs are isolated
using an alkaline lysis plasmid mini-preparation procedure and
analyzed by restriction endonuclease digest mapping to determine
the presence and orientation of the insert. This cloning strategy
yielded a pET29 expression construct comprising the polynucleotide
molecule encoding BoNT/A-33/A operably-linked to a carboxyl
terminal polyhistidine affinity binding peptide.
[1011] A similar cloning strategy is used to make pET29 expression
constructs comprising a polynucleotide molecule encoding for
BoNT/B-33/A, BoNT/C1-33/A, BoNT/D-33/A, BoNT/E-33/A, BoNT/F-33/A,
BoNT/G-33/A, TeNT-33/A, as well as the modified Clostridial toxin
indicated above comprising amino acids 10-144 of SEQ ID NO: 9;
amino acids 10-144 of SEQ ID NO: 10; amino acids 10-144 of SEQ ID
NO: 11; amino acids 10-146 of SEQ ID NO: 12; amino acids 10-144 of
SEQ ID NO: 13; amino acids 10-144 of SEQ ID NO: 14; amino acids
10-146 of SEQ ID NO: 15; amino acids 10-141 of SEQ ID NO: 16; amino
acids 10-141 of SEQ ID NO: 17; amino acids 10-141 of SEQ ID NO: 18;
amino acids 151-293 of SEQ ID NO: 10; amino acids 151-293 of SEQ ID
NO: 11; amino acids 153-294 of SEQ ID NO: 12; amino acids 151-279
of SEQ ID NO: 13; amino acids 151-292 of SEQ ID NO: 14; amino acids
153-291 of SEQ ID NO: 15; amino acids 148-285 of SEQ ID NO: 16;
amino acids 148-286 of SEQ ID NO: 17; amino acids 148-286 of SEQ ID
NO: 18; amino acids 9-146 of SEQ ID NO: 19; amino acids 9-146 of
SEQ ID NO: 20; amino acids 9-146 of SEQ ID NO: 21; amino acids
9-146 of SEQ ID NO: 22; amino acids 1050-1194 of SEQ ID NO: 23;
amino acids 1050-1199 of SEQ ID NO: 24; amino acids 1050-1194 of
SEQ ID NO: 25; amino acids 1049-1198 of SEQ ID NO: 26; amino acids
1049-1197 of SEQ ID NO: 27; amino acids 1049-1197 of SEQ ID NO: 28;
amino acids 1014-1163 of SEQ ID NO: 29; amino acids 1016-1160 of
SEQ ID NO: 30; amino acids 1017-1166 of SEQ ID NO: 31; and amino
acids 1050-1199 of SEQ ID NO: 32.
[1012] To construct a BoNT/A-33/A that replaces the BoNT/A
translocation facilitating domain with another Clostridial toxin
translocation facilitating domain, a translocation facilitating
domain of BoNT/B will be introduced into the BoNT/A-33/A as
described above using a Splicing by Overlapping ends polymerase
chain reaction (SOE-PCR) procedure, see, e.g., R. M. Horton et al.,
Engineering hybrid genes without the use of restriction enzymes:
gene splicing by overlapping extension, 77(1) Gene 61-68 (1989);
and R. M. Horton, PCR-mediated recombination and mutagenesis.
SOEing together tailor-made genes, 3(2) Mol. Biotechnol. 93-99
(1995). A nucleic acid fragment comprising a region encoding amino
acids 859 to 1097 of BoNT/B (SEQ ID NO: 2) will be operably-linked
by SOE-PCR to replace the region corresponding to the BoNT/A
translocation facilitating domain comprising amino acids 874-1110
of SEQ ID NO: 1 of the BoNT/A-33/A and will be subcloned into a
pCR2.1 vector using the TOPO.RTM. TA cloning method (Invitrogen,
Inc, Carlsbad, Calif.). The forward and reverse oligonucleotide
primers used for these reactions are designed to include unique
restriction enzyme sites useful for subsequent subcloning steps.
The resulting construct is digested with restriction enzymes that
1) excise the insert containing the entire open reading frame
encoding the modified BoNT/A-33/A; and 2) enable this insert to be
operably-linked to a pQBI-25A vector (Qbiogene, Inc., Carlsbad,
Calif.). The resulting restriction fragment will be purified by the
QIAquick Gel Extraction Kit (QIAGEN, Inc., Valencia, Calif.), and
will be subcloned using a T4 DNA ligase procedure into a pQBI-25A
vector (Qbiogene, Inc., Irvine, Calif.). This cloning strategy
yielded a pQBI-25 expression construct encoding a BoNT/A-33/A
comprising a BoNT/B translocation facilitating domain.
[1013] A similar cloning strategy is used to make pQBI-25
expression constructs comprising a polynucleotide molecule encoding
a BoNT/A-33/A that includes, e.g., a translocation facilitating
domain comprising amino acids 869-1111 of BoNT/C1 of SEQ ID NO: 3;
a translocation facilitating domain comprising amino acids 865-1098
of BoNT/D of SEQ ID NO: 4; a translocation facilitating domain
comprising amino acids 846-1058 of BoNT/E of SEQ ID NO: 5; a
translocation facilitating domain comprising amino acids 867-1105
of BoNT/F of SEQ ID NO: 6; a translocation facilitating domain
comprising amino acids 866-1105 of BoNT/G of SEQ ID NO: 7; or a
translocation facilitating domain comprising amino acids 882-1127
of TeNT of SEQ ID NO: 8, as well as the modified Clostridial toxin
indicated above comprising amino acids 10-144 of SEQ ID NO: 9;
amino acids 10-144 of SEQ ID NO: 10; amino acids 10-144 of SEQ ID
NO: 11; amino acids 10-146 of SEQ ID NO: 12; amino acids 10-144 of
SEQ ID NO: 13; amino acids 10-144 of SEQ ID NO: 14; amino acids
10-146 of SEQ ID NO: 15; amino acids 10-141 of SEQ ID NO: 16; amino
acids 10-141 of SEQ ID NO: 17; amino acids 10-141 of SEQ ID NO: 18;
amino acids 151-293 of SEQ ID NO: 10; amino acids 151-293 of SEQ ID
NO: 11; amino acids 153-294 of SEQ ID NO: 12; amino acids 151-279
of SEQ ID NO: 13; amino acids 151-292 of SEQ ID NO: 14; amino acids
153-291 of SEQ ID NO: 15; amino acids 148-285 of SEQ ID NO: 16;
amino acids 148-286 of SEQ ID NO: 17; amino acids 148-286 of SEQ ID
NO: 18; amino acids 9-146 of SEQ ID NO: 19; amino acids 9-146 of
SEQ ID NO: 20; amino acids 9-146 of SEQ ID NO: 21; amino acids
9-146 of SEQ ID NO: 22; amino acids 1050-1194 of SEQ ID NO: 23;
amino acids 1050-1199 of SEQ ID NO: 24; amino acids 1050-1194 of
SEQ ID NO: 25; amino acids 1049-1198 of SEQ ID NO: 26; amino acids
1049-1197 of SEQ ID NO: 27; amino acids 1049-1197 of SEQ ID NO: 28;
amino acids 1014-1163 of SEQ ID NO: 29; amino acids 1016-1160 of
SEQ ID NO: 30; amino acids 1017-1166 of SEQ ID NO: 31; and amino
acids 1050-1199 of SEQ ID NO: 32.
Example 3
Construction of a Modified Clostridial Toxin Comprising a
Translocation Facilitating Domain and a Non-Toxin Associated
Protein Fold
[1014] The following example illustrates how to make a modified
Clostridial toxin comprising a translocation facilitating domain
and an enhanced targeting domain comprising a non-toxin associated
protein fold.
[1015] A polynucleotide molecule encoding BoNT/A-17.gamma./A is
synthesized using standard procedures (BlueHeron.RTM.
Biotechnology, Bothell, Wash.), as described in Example 1.
BoNT/A-17.gamma./A is a BoNT/A modified to replace amino acids
1237-1296 of SEQ ID NO: 1, a .gamma.-fold from a BoNT/A
.beta.-trefoil domain, with amino acids 95 to 146 of SEQ ID NO: 19,
a .gamma.-fold from a HA-17 .beta.-trefoil domain from a
Clostridial botulinum serotype A strain. If desired, an expression
optimized polynucleotide molecule encoding BoNT/A-17.gamma./A can
be synthesized in order to improve expression in to a different
organism, such as, e.g., an Escherichia coli strain, a yeast
strain, an insect cell-line or a mammalian cell line, can be done,
see, e.g., Steward, supra, PCT Patent Serial No. 2005/020578 (Jun.
9, 2005); and Steward, supra, PCT Patent Serial No. 2005/027917
(Aug. 3, 2005). The synthesized polynucleotide molecule is verified
by sequencing using Big Dye Terminator.TM. Chemistry 3.1 (Applied
Biosystems, Foster City, Calif.) and an ABI 3100 sequencer (Applied
Biosystems, Foster City, Calif.).
[1016] A similar cloning strategy is used to make pUCBHB1 cloning
constructs for BoNT/B-17.gamma./A, a modified BoNT/B where amino
acids 1223-1291 of SEQ ID NO: 2 are replaced with amino acids 95 to
146 of SEQ ID NO: 19; BoNT/C1-F18.gamma., a modified BoNT/C1 where
amino acids 1230-1291 of SEQ ID NO: 3 are replaced with amino acids
95 to 146 of SEQ ID NO: 19; BoNT/D-17.gamma./A, a modified BoNT/D
where amino acids 1219-1276 of SEQ ID NO: 4 are replaced with amino
acids 95 to 146 of SEQ ID NO: 19; BoNT/E-17.gamma./A, a modified
BoNT/E where amino acids 1199-1252 of SEQ ID NO: 5 are replaced
with amino acids 95 to 146 of SEQ ID NO: 19; BoNT/F-17.gamma./A, a
modified BoNT/F where amino acids 1222-1274 of SEQ ID NO: 6 are
replaced with amino acids 95 to 146 of SEQ ID NO: 19;
BoNT/G-17.gamma./A, a modified BoNT/G where amino acids 1231-1297
of SEQ ID NO: 7 are replaced with amino acids 95 to 146 of SEQ ID
NO: 19; and TeNT-17.gamma./A, a modified TeNT where amino acids
1255-1315 of SEQ ID NO: 8 are replaced with amino acids 95 to 146
of SEQ ID NO: 19.
[1017] Similarly, the .gamma.-fold from a HA-17/A .beta.-trefoil
domain can replace amino acids 1111-1162 of SEQ ID NO: 1, an
.alpha.-fold from a BoNT/A .beta.-trefoil domain; amino acids
1098-1147 of SEQ ID NO: 2, an .alpha.-fold from a BoNT/B
.beta.-trefoil domain; amino acids 1112-1150 of SEQ ID NO: 3, an
.alpha.-fold from a BoNT/C1 trefoil domain; amino acids 1099-1137
of SEQ ID NO: 4, an .alpha.-fold from a BoNT/D .beta.-trefoil
domain; amino acids 1086-1129 of SEQ ID NO: 5, an .alpha.-fold from
a BoNT/E .beta.-trefoil domain; amino acids 1106-1152 of SEQ ID NO:
6, an .alpha.-fold from a BoNT/F .beta.-trefoil domain; amino acids
1106-1153 of SEQ ID NO: 7, an .alpha.-fold from a BoNT/G
.beta.-trefoil domain; or amino acids 1128-1177 of SEQ ID NO: 8, an
.alpha.-fold from a TeNT .beta.-trefoil domain. Similarly, the
.gamma.-fold from a HA-17/A.beta.-trefoil domain can replace amino
acids 1179-1223 of SEQ ID NO: 1, a .beta.-fold from a BoNT/A
.beta.-trefoil domain; amino acids 1166-1210 of SEQ ID NO: 2, a
.beta.-fold from a BoNT/B .beta.-trefoil domain; amino acids
1167-1218 of SEQ ID NO: 3, a .beta.-fold from a BoNT/C1
.beta.-trefoil domain; amino acids 1154-1207 of SEQ ID NO: 4, a
.beta.-fold from a BoNT/D .beta.-trefoil domain; amino acids
1147-1190 of SEQ ID NO: 5, a .beta.-fold from a BoNT/E
.beta.-trefoil domain; amino acids 1172-1213 of SEQ ID NO: 6, a
.beta.-fold from a BoNT/F .beta.-trefoil domain; amino acids
1173-1218 of SEQ ID NO: 7, a .beta.-fold from a BoNT/G
.beta.-trefoil domain; or amino acids 1195-1240 of SEQ ID NO: 8, a
.beta.-fold from a TeNT .beta.-trefoil domain.
[1018] Similarly, an .alpha.-fold, .beta.-fold or .gamma.-fold from
a .beta.-trefoil domain from a Clostridial toxin indicated above
can be replaced with a HA-17 .gamma.-fold comprising amino acids
95-146 of SEQ ID NO: 20, amino acids 95-146 of SEQ ID NO: 21, or
amino acids 95-146 of SEQ ID NO: 22; a HA-33 .gamma.-fold
comprising amino acids 105-144 of SEQ ID NO: 9, amino acids 105-144
of SEQ ID NO: 10, amino acids 105-144 of SEQ ID NO: 11, amino acids
107-146 of SEQ ID NO: 12, amino acids 105-144 of SEQ ID NO: 13,
amino acids 105-144 of SEQ ID NO: 14, amino acids 107-146 of SEQ ID
NO: 15, amino acids 103-141 of SEQ ID NO: 16, amino acids 103-141
of SEQ ID NO: 17, amino acids 103-141 of SEQ ID NO: 18, amino acids
249-293 of SEQ ID NO: 9, amino acids 249-293 of SEQ ID NO: 10,
amino acids 249-293 of SEQ ID NO: 11, amino acids 250-294 of SEQ ID
NO: 12, amino acids 249-279 of SEQ ID NO: 13, amino acids 248-292
of SEQ ID NO: 14, amino acids 249-291 of SEQ ID NO: 15, amino acids
241-285 of SEQ ID NO: 16, amino acids 242-286 of SEQ ID NO: 17, or
amino acids 242-286 of SEQ ID NO: 18; or a NTNH .gamma.-fold
comprising amino acids 1149-1194 of SEQ ID NO: 23, amino acids
1149-1199 of SEQ ID NO: 24, amino acids 1149-1194 of SEQ ID NO: 25,
amino acids 1148-1198 of SEQ ID NO: 26, amino acids 1148-1197 of
SEQ ID NO: 27, amino acids 1148-1197 of SEQ ID NO: 28, amino acids
1114-1163 of SEQ ID NO: 29, amino acids 1115-1160 of SEQ ID NO: 30,
amino acids 1117-1166 of SEQ ID NO: 31, or amino acids 1150-1199 of
SEQ ID NO: 32.
[1019] Likewise, an .alpha.-fold, .beta.-fold or .gamma.-fold from
a .beta.-trefoil domain from a Clostridial toxin indicated above
can be replaced with a HA-17 .alpha.-fold comprising amino acids
9-50 of SEQ ID NO: 19, amino acids 9-50 of SEQ ID NO: 20, amino
acids 9-50 of SEQ ID NO: 21, or amino acids 9-50 of SEQ ID NO: 22;
a HA-33 .alpha.-fold comprising amino acids 10-54 of SEQ ID NO: 9,
amino acids 10-54 of SEQ ID NO: 10, amino acids 10-54 of SEQ ID NO:
11, amino acids 10-56 of SEQ ID NO: 12, amino acids 10-54 of SEQ ID
NO: 13, amino acids 10-54 of SEQ ID NO: 14, amino acids 10-56 of
SEQ ID NO: 15, amino acids 10-54 of SEQ ID NO: 16, amino acids
10-54 of SEQ ID NO: 17, amino acids 10-54 of SEQ ID NO: 18, amino
acids 151-195 of SEQ ID NO: 9, amino acids 151-195 of SEQ ID NO:
10, amino acids 151-195 of SEQ ID NO: 11, amino acids 153-197 of
SEQ ID NO: 12, amino acids 151-195 of SEQ ID NO: 13, amino acids
151-195 of SEQ ID NO: 14, amino acids 153-197 of SEQ ID NO: 15,
amino acids 148-190 of SEQ ID NO: 16, amino acids 148-190 of SEQ ID
NO: 17, or amino acids 148-190 of SEQ ID NO: 18; or a NTNH
.alpha.-fold comprising amino acids 1050-1097 of SEQ ID NO: 23,
amino acids 1050-1097 of SEQ ID NO: 24, amino acids 1050-1097 of
SEQ ID NO: 25, amino acids 1049-1096 of SEQ ID NO: 26, amino acids
1049-1096 of SEQ ID NO: 27, amino acids 1049-1096 of SEQ ID NO: 28,
amino acids 1014-1061 of SEQ ID NO: 29, amino acids 1016-1063 of
SEQ ID NO: 30, amino acids 1017-1064 of SEQ ID NO: 31, or amino
acids 1050-1097 of SEQ ID NO: 32.
[1020] Further, an .alpha.-fold, .beta.-fold or .gamma.-fold from a
.beta.-trefoil domain from a Clostridial toxin indicated above can
be replaced with a HA-17 .beta.-fold comprising amino acids 55-91
of SEQ ID NO: 19, amino acids 55-91 of SEQ ID NO: 20, amino acids
55-91 of SEQ ID NO: 21, or amino acids 55-91 of SEQ ID NO: 22; a
HA-33 .beta.-fold comprising amino acids 60-100 of SEQ ID NO: 9,
amino acids 60-100 of SEQ ID NO: 10, amino acids 60-100 of SEQ ID
NO: 11, amino acids 62-102 of SEQ ID NO: 12, amino acids 60-100 of
SEQ ID NO: 13, amino acids 60-100 of SEQ ID NO: 14, amino acids
62-102 of SEQ ID NO: 15, amino acids 60-98 of SEQ ID NO: 16, amino
acids 60-98 of SEQ ID NO: 17, amino acids 60-98 of SEQ ID NO: 18,
amino acids 200-242 of SEQ ID NO: 9, amino acids 200-242 of SEQ ID
NO: 10, amino acids 200-242 of SEQ ID NO: 11, amino acids 202-243
of SEQ ID NO: 12, amino acids 200-242 of SEQ ID NO: 13, amino acids
200-241 of SEQ ID NO: 14, amino acids 200-242 of SEQ ID NO: 15,
amino acids 195-234 of SEQ ID NO: 16, amino acids 195-235 of SEQ ID
NO: 17, or amino acids 195-235 of SEQ ID NO: 18; or a NTNH
.beta.-fold comprising amino acids 1111-1138 of SEQ ID NO: 23,
amino acids 1111-1139 of SEQ ID NO: 24, amino acids 1111-1138 of
SEQ ID NO: 25, amino acids 1110-1138 of SEQ ID NO: 26, amino acids
1110-1138 of SEQ ID NO: 27, amino acids 1110-1138 of SEQ ID NO: 28,
amino acids 1075-1103 of SEQ ID NO: 29, amino acids 1077-1104 of
SEQ ID NO: 30, amino acids 1078-1106 of SEQ ID NO: 31, or amino
acids 1111-1139 of SEQ ID NO: 32.
[1021] To construct pET29/BoNT/A-17.gamma./A, a
pUCBHB1/BoNT/A-17.gamma./A construct is digested with restriction
endonucleases that 1) excise the insert comprising the open reading
frame encoding BoNT/A-17.gamma./A; and 2) enable this insert to be
operably-linked to a pET29 vector (EMD Biosciences-Novagen,
Madison, Wis.). This insert is subcloned using a T4 DNA ligase
procedure into a pET29 vector that is digested with appropriate
restriction endonucleases to yield pET29/BoNT/A-17.gamma./A. The
ligation mixture is transformed into chemically competent E. coli
DH5.alpha. cells (Invitrogen, Inc, Carlsbad, Calif.) using a heat
shock method, plated on 1.5% Luria-Bertani agar plates (pH 7.0)
containing 50 .mu.g/mL of Kanamycin, and placed in a 37.degree. C.
incubator for overnight growth. Bacteria containing expression
constructs are identified as Kanamycin resistant colonies.
Candidate constructs are isolated using an alkaline lysis plasmid
mini-preparation procedure and analyzed by restriction endonuclease
digest mapping to determine the presence and orientation of the
insert. This cloning strategy yielded a pET29 expression construct
comprising the polynucleotide molecule encoding BoNT/A-17.gamma./A
operably-linked to a carboxyl terminal polyhistidine affinity
binding peptide.
[1022] A similar cloning strategy is used to make pET29 expression
constructs comprising a polynucleotide molecule encoding
BoNT/B-17.gamma./A, BoNT/C1-17.gamma./A, BoNT/D-17.gamma./A,
BoNT/E-17.gamma./A, BoNT/F-17.gamma./A, BoNT/G-17.gamma./A,
TeNT-17.gamma./A, BoNT/A-33-1.gamma./A, BoNT/B-33-1.gamma./A,
BoNT/C1-33-1.gamma./A, BoNT/D-33-1.gamma./A, BoNT/E-33-1 .gamma./A,
BoNT/F-33-1.gamma./A, BoNT/G-33-1.gamma./A, TeNT-33-1.gamma./A,
BoNT/A-33-2.gamma./A, BoNT/B-33-2.gamma./A, BoNT/C1-33-2.gamma./A,
BoNT/D-33-2.gamma./A, BoNT/E-33-2.gamma./A, BoNT/F-33-2.gamma./A,
BoNT/G-33-2.gamma./A, TeNT-33-2.gamma./A, BoNT/B-NTNH.gamma./A,
BoNT/B-NTNH.gamma./A, BoNT/C1-NTNH.gamma./A, BoNT/D-NTNH.gamma./A,
BoNT/E-NTNH.gamma./A, BoNT/F-NTNH.gamma./A, BoNT/G-NTNH.gamma./A,
TeNT-NTNH.gamma./A, BoNT/A-17.alpha./A, BoNT/B-17.alpha./A,
BoNT/C1-17.alpha./A, BoNT/D-17.alpha./A, BoNT/E-17.alpha./A,
BoNT/F-17.alpha./A, BoNT/G-17.alpha./A, TeNT-17.alpha./A,
BoNT/A-33-1.alpha./A, BoNT/B-33-1.alpha./A, BoNT/C1-33-1.alpha./A,
BoNT/D-33-1.alpha./A, BoNT/E-33-1.alpha./A, BoNT/F-33-1.alpha./A,
BoNT/G-33-1.alpha./A, TeNT-33-1.alpha./A, BoNT/A-33-2.alpha./A,
BoNT/B-33-2.alpha./A, BoNT/C1-33-2.alpha./A, BoNT/D-33-2.alpha./A,
BoNT/E-33-2.alpha./A, BoNT/F-33-2.alpha./A, BoNT/G-33-2.alpha./A,
TeNT-33-2.alpha./A, BoNT/B-NTNH.alpha./A, BoNT/B-NTNH.alpha./A,
BoNT/C1-NTNH.alpha./A, BoNT/D-NTNH.alpha./A, BoNT/E-NTNH.alpha./A,
BoNT/F-NTNH.alpha./A, BoNT/G-NTNH.alpha./A, TeNT-NTNHa,
BoNT/A-17.beta./A, BoNT/B-17.beta./A, BoNT/C1-17.beta./A,
BoNT/D-17.beta./A, BoNT/E-17.beta./A, BoNT/F-17.beta./A,
BoNT/G-17.beta./A, TeNT-17.beta./A, BoNT/A-33-1.beta./A,
BoNT/B-33-1.beta./A, BoNT/C1-33-1.beta./A, BoNT/D-33-1.beta./A,
BoNT/E-33-1.beta./A, BoNT/F-33-1.beta./A, BoNT/G-33-1.beta./A,
TeNT-33-1.beta./A, BoNT/A-33-2.beta./A, BoNT/B-33-2.beta./A,
BoNT/C1-33-2.beta./A, BoNT/D-33-2.beta./A, BoNT/E-33-2.beta./A,
BoNT/F-33-2.beta./A, BoNT/G-33-2.beta./A, TeNT-33-2.beta./A,
BoNT/B-NTNH.beta./A, BoNT/B-NTNH.beta./A, BoNT/C1-NTNH.beta./A,
BoNT/D-NTNH.beta./A, BoNT/E-NTNH.beta./A, BoNT/F-NTNH.beta./A,
BoNT/G-NTNH.beta./A, TeNT-NTNH.beta..
[1023] A similar cloning strategy is also used to make pET29
expression constructs comprising a polynucleotide molecule encoding
a modified Clostridial toxin indicated above that has its
.alpha.-fold or .beta.-fold replaced by amino acids comprising a
HA-17 .alpha.-fold, .beta.-fold or .gamma.-fold; amino acids
comprising a HA-33 1.alpha.-fold, 1.beta.-fold, 1.gamma.-fold,
2.alpha.-fold, 26-fold, or 2.gamma.-fold; or amino acids comprising
a NTNH .alpha.-fold, .beta.-fold or .gamma.-fold.
[1024] To construct a BoNT/A-17.gamma./A that replaces the BoNT/A
translocation facilitating domain with another Clostridial toxin
translocation facilitating domain, a translocation facilitating
domain of BoNT/B will be introduced into the BoNT/A-17.gamma./A as
described above using a Splicing by Overlapping ends polymerase
chain reaction (SOE-PCR) procedure, see, e.g., R. M. Horton et al.,
Engineering hybrid genes without the use of restriction enzymes:
gene splicing by overlapping extension, 77(1) Gene 61-68 (1989);
and R. M. Horton, PCR-mediated recombination and mutagenesis.
SOEing together tailor-made genes, 3(2) Mol. Biotechnol. 93-99
(1995). A nucleic acid fragment comprising a region encoding amino
acids 859 to 1097 of BoNT/B (SEQ ID NO: 2) will be operably-linked
by SOE-PCR to replace the region corresponding to the BoNT/A
translocation facilitating domain comprising amino acids 874-1110
of SEQ ID NO: 1 of the BoNT/A-17.gamma./A and will be subcloned
into a pCR2.1 vector using the TOPO.RTM. TA cloning method
(Invitrogen, Inc, Carlsbad, Calif.). The forward and reverse
oligonucleotide primers used for these reactions are designed to
include unique restriction enzyme sites useful for subsequent
subcloning steps. The resulting construct is digested with
restriction enzymes that 1) excise the insert containing the entire
open reading frame encoding the modified BoNT/A-17.gamma./A; and 2)
enable this insert to be operably-linked to a pQBI-25A vector
(Qbiogene, Inc., Carlsbad, Calif.). The resulting restriction
fragment will be purified by the QIAquick Gel Extraction Kit
(QIAGEN, Inc., Valencia, Calif.), and will be subcloned using a T4
DNA ligase procedure into a pQBI-25A vector (Qbiogene, Inc.,
Irvine, Calif.). This cloning strategy yielded a pQBI-25 expression
construct encoding a BoNT/A-17.gamma./A comprising a BoNT/B
translocation facilitating domain.
[1025] A similar cloning strategy is used to make pQBI-25
expression constructs comprising a polynucleotide molecule encoding
a BoNT/A-17.gamma./A that includes, e.g., a translocation
facilitating domain comprising amino acids 869-1111 of BoNT/C1 of
SEQ ID NO: 3; a translocation facilitating domain comprising amino
acids 865-1098 of BoNT/D of SEQ ID NO: 4; a translocation
facilitating domain comprising amino acids 846-1058 of BoNT/E of
SEQ ID NO: 5; a translocation facilitating domain comprising amino
acids 867-1105 of BoNT/F of SEQ ID NO: 6; a translocation
facilitating domain comprising amino acids 866-1105 of BoNT/G of
SEQ ID NO: 7; or a translocation facilitating domain comprising
amino acids 882-1127 of TeNT of SEQ ID NO: 8.
[1026] Likewise, as well as, a polynucleotide molecule encoding a
Clostridial translocation facilitating domain as described above
can be introduced into a polynucleotide molecule encoding
BoNT/B-17.gamma./A, BoNT/C1-17.gamma./A, BoNT/D-17.gamma./A,
BoNT/E-17.gamma./A, BoNT/F-17.gamma./A, BoNT/G-17.gamma./A,
TeNT-17.gamma./A, BoNT/A-33-1.gamma./A, BoNT/B-33-1 .gamma./A,
BoNT/C1-33-1.gamma./A, BoNT/D-33-1 .gamma./A, BoNT/E-33-1
.gamma./A, BoNT/F-33-1.gamma./A, BoNT/G-33-1.gamma./A,
TeNT-33-1.gamma./A, BoNT/A-33-2.gamma./A, BoNT/B-33-2.gamma./A,
BoNT/C1-33-2.gamma./A, BoNT/D-33-2.gamma./A, BoNT/E-33-2.gamma./A,
BoNT/F-33-2.gamma./A, BoNT/G-33-2.gamma./A, TeNT-33-2.gamma./A,
BoNT/B-NTNH.gamma./A, BoNT/B-NTNH.gamma./A, BoNT/C1-NTNH.gamma./A,
BoNT/D-NTNH.gamma./A, BoNT/E-NTNH.gamma./A, BoNT/F-NTNH.gamma./A,
BoNT/G-NTNH.gamma./A, TeNT-NTNH.gamma./A, BoNT/A-17.alpha./A,
BoNT/B-17.alpha./A, BoNT/C1-17.alpha./A, BoNT/D-17.alpha./A,
BoNT/E-17.alpha./A, BoNT/F-17.alpha./A, BoNT/G-17.alpha./A,
TeNT-17.alpha./A, BoNT/A-33-1.alpha./A, BoNT/B-33-1.alpha./A,
BoNT/C1-33-1.alpha./A, BoNT/D-33-1.alpha./A, BoNT/E-33-1.alpha./A,
BoNT/F-33-1.alpha./A, BoNT/G-33-1.alpha./A, TeNT-33-1.alpha./A,
BoNT/A-33-2.alpha./A, BoNT/B-33-2.alpha./A, BoNT/C1-33-2.alpha./A,
BoNT/D-33-2.alpha./A, BoNT/E-33-2.alpha./A, BoNT/F-33-2.alpha./A,
BoNT/G-33-2.alpha./A, TeNT-33-2.alpha./A, BoNT/B-NTNH.alpha./A,
BoNT/B-NTNH.alpha./A, BoNT/C1-NTNH.alpha./A, BoNT/D-NTNH.alpha./A,
BoNT/E-NTNH.alpha./A, BoNT/F-NTNH.alpha./A, BoNT/G-NTNH.alpha./A,
TeNT-NTNHa, BoNT/A-17.beta./A, BoNT/B-17.beta./A,
BoNT/C1-17.beta./A, BoNT/D-17.beta./A, BoNT/E-17.beta./A,
BoNT/F-17.beta./A, BoNT/G-17.beta./A, TeNT-17.beta./A,
BoNT/A-33-1.beta./A, BoNT/B-33-1.beta./A, BoNT/C1-33-1.beta./A,
BoNT/D-33-1.beta./A, BoNT/E-33-1.beta./A, BoNT/F-33-1.beta./A,
BoNT/G-33-1.beta./A, TeNT-33-1.beta./A, BoNT/A-33-2.beta./A,
BoNT/B-33-2.beta./A, BoNT/C1-33-2.beta./A, BoNT/D-33-2.beta./A,
BoNT/E-33-2.beta./A, BoNT/F-33-2.beta./A, BoNT/G-33-2.beta./A,
TeNT-33-2.beta./A, BoNT/B-NTNH.beta./A, BoNT/B-NTNH.beta./A,
BoNT/C1-NTNH.beta./A, BoNT/D-NTNH.beta./A, BoNT/E-NTNH.beta./A,
BoNT/F-NTNH.beta./A, BoNT/G-NTNH.beta./A, TeNT-NTNH.beta..
Example 4
Construction of a Modified Clostridial Toxin Comprising a
Translocation Facilitating Domain and a FGF that Selectively Binds
to a FGFR3
[1027] The following example illustrates how to make a modified
Clostridial toxin comprising a translocation facilitating domain
and an enhanced targeting domain comprising a FGF that selectively
binds to a FGFR3.
[1028] A polynucleotide molecule encoding BoNT/A-F18 is synthesized
using standard procedures (BlueHeron.RTM. Biotechnology, Bothell,
Wash.), as described in Example 1. BoNT/A-F18 is a BoNT/A modified
to replace amino acids 1111-1296 of SEQ ID NO: 1, a BoNT/A
.beta.-trefoil domain, with amino acids 54 to 183 of SEQ ID NO: 39,
a FGF-18 .beta.-trefoil domain. If desired, an expression optimized
polynucleotide molecule encoding BoNT/A-F18 can be synthesized in
order to improve expression in to a different organism, such as,
e.g., an Escherichia coli strain, a yeast strain, an insect
cell-line or a mammalian cell line, can be done, see, e.g.,
Steward, supra, PCT Patent Serial No. 2005/020578 (Jun. 9, 2005);
and Steward, supra, PCT Patent Serial No. 2005/027917 (Aug. 3,
2005). The synthesized polynucleotide molecule is verified by
sequencing using Big Dye Terminator.TM. Chemistry 3.1 (Applied
Biosystems, Foster City, Calif.) and an ABI 3100 sequencer (Applied
Biosystems, Foster City, Calif.).
[1029] A similar cloning strategy is used to make pUCBHB1 cloning
constructs for BoNT/B-F18, a modified BoNT/B where amino acids
1098-1291 of SEQ ID NO: 2 are replaced with amino acids 54 to 183
of SEQ ID NO: 39; BoNT/C1-F18, a modified BoNT/C1 where amino acids
1112-1291 of SEQ ID NO: 3 are replaced with amino acids 54 to 183
of SEQ ID NO: 39; BoNT/D-F18, a modified BoNT/D where amino acids
1099-1276 of SEQ ID NO: 4 are replaced with amino acids 54 to 183
of SEQ ID NO: 39; BoNT/E-F18, a modified BoNT/E where amino acids
1086-1252 of SEQ ID NO: 5 are replaced with amino acids 54 to 183
of SEQ ID NO: 39; BoNT/F-F18, a modified BoNT/F where amino acids
1106-1274 of SEQ ID NO: 6 are replaced with amino acids 54 to 183
of SEQ ID NO: 39; BoNT/G-F18, a modified BoNT/G where amino acids
1106-1297 of SEQ ID NO: 7 are replaced with amino acids 54 to 183
of SEQ ID NO: 39; and TeNT-F18, a modified TeNT where amino acids
1128-1315 of SEQ ID NO: 8 are replaced with amino acids 54 to 183
of SEQ ID NO: 39. Similarly, the .beta.-trefoil domain from a
Clostridial toxin indicated above can be replaced with a FGF
.beta.-trefoil domain comprising amino acids 26-155 of SEQ ID NO:
33; amino acids 29-155 of SEQ ID NO: 34; amino acids 83-206 of SEQ
ID NO: 35; amino acids 43-172 of SEQ ID NO: 36; amino acids 63-196
of SEQ ID NO: 37; amino acids 55-183 of SEQ ID NO: 38; and amino
acids 54-183 of SEQ ID NO: 39.
[1030] To construct pET29/BoNT/A-F18, a pUCBHB1/BoNT/A-F18
construct is digested with restriction endonucleases that 1) excise
the insert comprising the open reading frame encoding BoNT/A-F18;
and 2) enable this insert to be operably-linked to a pET29 vector
(EMD Biosciences-Novagen, Madison, Wis.). This insert is subcloned
using a T4 DNA ligase procedure into a pET29 vector that is
digested with appropriate restriction endonucleases to yield
pET29/BoNT/A-F18. The ligation mixture is transformed into
chemically competent E. coli DH5.alpha. cells (Invitrogen, Inc,
Carlsbad, Calif.) using a heat shock method, plated on 1.5%
Luria-Bertani agar plates (pH 7.0) containing 50 .mu.g/mL of
Kanamycin, and placed in a 37.degree. C. incubator for overnight
growth. Bacteria containing expression constructs are identified as
Kanamycin resistant colonies. Candidate constructs are isolated
using an alkaline lysis plasmid mini-preparation procedure and
analyzed by restriction endonuclease digest mapping to determine
the presence and orientation of the insert. This cloning strategy
yielded a pET29 expression construct comprising the polynucleotide
molecule encoding BoNT/A-F18 operably-linked to a carboxyl terminal
polyhistidine affinity binding peptide.
[1031] A similar cloning strategy is used to make pET29 expression
constructs comprising a polynucleotide molecule encoding for
BoNT/B-F18, BoNT/C1-F18, BoNT/D-F18, BoNT/E-F18, BoNT/F-F18,
BoNT/G-F18, TeNT-F18, as well as the modified Clostridial toxin
indicated above comprising amino acids 26-155 of SEQ ID NO: 33;
amino acids 29-155 of SEQ ID NO: 34; amino acids 83-206 of SEQ ID
NO: 35; amino acids 43-172 of SEQ ID NO: 36; amino acids 63-196 of
SEQ ID NO: 37; amino acids 55-183 of SEQ ID NO: 38; and amino acids
54-183 of SEQ ID NO: 39.
[1032] To construct a BoNT/A-F18 that replaces the BoNT/A
translocation facilitating domain with another Clostridial toxin
translocation facilitating domain, a translocation facilitating
domain of BoNT/B will be introduced into the BoNT/A-F18 as
described above using a Splicing by Overlapping ends polymerase
chain reaction (SOE-PCR) procedure, see, e.g., R. M. Horton et al.,
Engineering hybrid genes without the use of restriction enzymes:
gene splicing by overlapping extension, 77(1) Gene 61-68 (1989);
and R. M. Horton, PCR-mediated recombination and mutagenesis.
SOEing together tailor-made genes, 3(2) Mol. Biotechnol. 93-99
(1995). A nucleic acid fragment comprising a region encoding amino
acids 859 to 1097 of BoNT/B (SEQ ID NO: 2) will be operably-linked
by SOE-PCR to replace the region corresponding to the BoNT/A
translocation facilitating domain comprising amino acids 874-1110
of SEQ ID NO: 1 of the BoNT/A-F18 and will be subcloned into a
pCR2.1 vector using the TOPO.RTM. TA cloning method (Invitrogen,
Inc, Carlsbad, Calif.). The forward and reverse oligonucleotide
primers used for these reactions are designed to include unique
restriction enzyme sites useful for subsequent subcloning steps.
The resulting construct is digested with restriction enzymes that
1) excise the insert containing the entire open reading frame
encoding the modified BoNT/A-F18; and 2) enable this insert to be
operably-linked to a pQBI-25A vector (Qbiogene, Inc., Carlsbad,
Calif.). The resulting restriction fragment will be purified by the
QIAquick Gel Extraction Kit (QIAGEN, Inc., Valencia, Calif.), and
will be subcloned using a T4 DNA ligase procedure into a pQBI-25A
vector (Qbiogene, Inc., Irvine, Calif.). This cloning strategy
yielded a pQBI-25 expression construct encoding a BoNT/A-F18
comprising a BoNT/B translocation facilitating domain.
[1033] A similar cloning strategy is used to make pQBI-25
expression constructs comprising a polynucleotide molecule encoding
a BoNT/A-F18 that includes, e.g., a translocation facilitating
domain comprising amino acids 869-1111 of BoNT/C1 of SEQ ID NO: 3;
a translocation facilitating domain comprising amino acids 865-1098
of BoNT/D of SEQ ID NO: 4; a translocation facilitating domain
comprising amino acids 846-1058 of BoNT/E of SEQ ID NO: 5; a
translocation facilitating domain comprising amino acids 867-1105
of BoNT/F of SEQ ID NO: 6; a translocation facilitating domain
comprising amino acids 866-1105 of BoNT/G of SEQ ID NO: 7; or a
translocation facilitating domain comprising amino acids 882-1127
of TeNT of SEQ ID NO: 8.
[1034] Likewise, as well as, a polynucleotide molecule encoding a
Clostridial translocation facilitating domain as described above
can be introduced into a polynucleotide molecule encoding
BoNT/B-F18, BoNT/C1-F18, BoNT/D-F18, BoNT/E-F18, BoNT/F-F18,
BoNT/G-F18, TeNT-F18, as well as the modified Clostridial toxin
indicated above comprising amino acids 26-155 of SEQ ID NO: 33;
amino acids 29-155 of SEQ ID NO: 34; amino acids 83-206 of SEQ ID
NO: 35; amino acids 43-172 of SEQ ID NO: 36; amino acids 63-196 of
SEQ ID NO: 37; amino acids 55-183 of SEQ ID NO: 38; and amino acids
54-183 of SEQ ID NO: 39.
Example 5
Construction of a Modified Clostridial Toxin Comprising a
Translocation Facilitating Domain and a FGF Fold that Selectively
Binds to a FGFR3
[1035] The following example illustrates how to make a modified
Clostridial toxin comprising a translocation facilitating domain
and an enhanced targeting domain comprising a FGF fold that
selectively binds to a FGFR3.
[1036] A polynucleotide molecule encoding BoNT/A-F18.gamma. is
synthesized using standard procedures (BlueHeron.RTM.
Biotechnology, Bothell, Wash.), as described in Example 1.
BoNT/A-F18.gamma. is a BoNT/A modified to replace amino acids
1237-1296 of SEQ ID NO: 1, a .gamma.-fold from a BoNT/A
.beta.-trefoil domain, with amino acids 138 to 183 of SEQ ID NO:
39, a .gamma.-fold from a FGF-18 .beta.-trefoil domain. If desired,
an expression optimized polynucleotide molecule encoding
BoNT/A-F18.gamma. can be synthesized in order to improve expression
in to a different organism, such as, e.g., an Escherichia coli
strain, a yeast strain, an insect cell-line or a mammalian cell
line, can be done, see, e.g., Steward, supra, PCT Patent Serial No.
2005/020578 (Jun. 9, 2005); and Steward, supra, PCT Patent Serial
No. 2005/027917 (Aug. 3, 2005). The synthesized polynucleotide
molecule is verified by sequencing using Big Dye Terminator.TM.
Chemistry 3.1 (Applied Biosystems, Foster City, Calif.) and an ABI
3100 sequencer (Applied Biosystems, Foster City, Calif.).
[1037] A similar cloning strategy is used to make pUCBHB1 cloning
constructs for BoNT/B-F18.gamma., a modified BoNT/B where amino
acids 1223-1291 of SEQ ID NO: 2 are replaced with amino acids 138
to 183 of SEQ ID NO: 39; BoNT/C1-F18.gamma., a modified BoNT/C1
where amino acids 1230-1291 of SEQ ID NO: 3 are replaced with amino
acids 138 to 183 of SEQ ID NO: 39; BoNT/D-F18.gamma., a modified
BoNT/D where amino acids 1219-1276 of SEQ ID NO: 4 are replaced
with amino acids 138 to 183 of SEQ ID NO: 39; BoNT/E-F18.gamma., a
modified BoNT/E where amino acids 1199-1252 of SEQ ID NO: 5 are
replaced with amino acids 138 to 183 of SEQ ID NO: 39;
BoNT/F-F18.gamma., a modified BoNT/F where amino acids 1222-1274 of
SEQ ID NO: 6 are replaced with amino acids 138 to 183 of SEQ ID NO:
39; BoNT/G-F18.gamma., a modified BoNT/G where amino acids
1231-1297 of SEQ ID NO: 7 are replaced with amino acids 138 to 183
of SEQ ID NO: 39; and TeNT-F18.gamma., a modified TeNT where amino
acids 1255-1315 of SEQ ID NO: 8 are replaced with amino acids 138
to 183 of SEQ ID NO: 39. Similarly, the .gamma.-fold from a FGF-18
.beta.-trefoil domain can replace amino acids 1111-1162 of SEQ ID
NO: 1, an .alpha.-fold from a BoNT/A .beta.-trefoil domain; amino
acids 1098-1147 of SEQ ID NO: 2, an .alpha.-fold from a BoNT/B
.beta.-trefoil domain; amino acids 1112-1150 of SEQ ID NO: 3, an
.alpha.-fold from a BoNT/C1 .beta.-trefoil domain; amino acids
1099-1137 of SEQ ID NO: 4, an .alpha.-fold from a BoNT/D
.beta.-trefoil domain; amino acids 1086-1129 of SEQ ID NO: 5, an
.alpha.-fold from a BoNT/E .beta.-trefoil domain; amino acids
1106-1152 of SEQ ID NO: 6, an .alpha.-fold from a BoNT/F
.beta.-trefoil domain; amino acids 1106-1153 of SEQ ID NO: 7, an
.alpha.-fold from a BoNT/G .beta.-trefoil domain; or amino acids
1128-1177 of SEQ ID NO: 8, an .alpha.-fold from a TeNT
.beta.-trefoil domain. Similarly, the .gamma.-fold from a FGF-18
.beta.-trefoil domain can replace amino acids 1179-1223 of SEQ ID
NO: 1, a .beta.-fold from a BoNT/A trefoil domain; amino acids
1166-1210 of SEQ ID NO: 2, a .beta.-fold from a BoNT/B
.beta.-trefoil domain; amino acids 1167-1218 of SEQ ID NO: 3, a
.beta.-fold from a BoNT/C1 .beta.-trefoil domain; amino acids
1154-1207 of SEQ ID NO: 4, a .beta.-fold from a BoNT/D
.beta.-trefoil domain; amino acids 1147-1190 of SEQ ID NO: 5, a
.beta.-fold from a BoNT/E .beta.-trefoil domain; amino acids
1172-1213 of SEQ ID NO: 6, a .beta.-fold from a BoNT/F trefoil
domain; amino acids 1173-1218 of SEQ ID NO: 7, a .beta.-fold from a
BoNT/G .beta.-trefoil domain; or amino acids 1195-1240 of SEQ ID
NO: 8, a .beta.-fold from a TeNT .beta.-trefoil domain.
[1038] Similarly, an .alpha.-fold, .beta.-fold or .gamma.-fold from
a .beta.-trefoil domain from a Clostridial toxin indicated above
can be replaced with a FGF .gamma.-fold comprising amino acids
109-155 of SEQ ID NO: 33; amino acids 115-155 of SEQ ID NO: 34;
amino acids 166-206 of SEQ ID NO: 35; amino acids 127-172 of SEQ ID
NO: 36; amino acids 148-196 of SEQ ID NO: 37; or amino acids
138-183 of SEQ ID NO: 38. Likewise, an .alpha.-fold, .beta.-fold or
.gamma.-fold from a .beta.-trefoil domain from a Clostridial toxin
indicated above can be replaced with a FGF .alpha.-fold comprising
amino acids 26-64 of SEQ ID NO: 33; amino acids 29-67 of SEQ ID NO:
34; amino acids 83-121 of SEQ ID NO: 35; amino acids 43-80 of SEQ
ID NO: 36; amino acids 63-100 of SEQ ID NO: 37; amino acids 55-91
of SEQ ID NO: 38; or amino acids 54-91 of SEQ ID NO: 39. Further,
an .alpha.-fold, .beta.-fold or .gamma.-fold from a .beta.-trefoil
domain from a Clostridial toxin indicated above can be replaced
with a FGF .beta.-fold comprising amino acids 68-105 of SEQ ID NO:
33; amino acids 71-111 of SEQ ID NO: 34; amino acids 125-162 of SEQ
ID NO: 35; amino acids 84-123 of SEQ ID NO: 36; amino acids 104-144
of SEQ ID NO: 37; amino acids 95-134 of SEQ ID NO: 38; or amino
acids 95-134 of SEQ ID NO: 39.
[1039] To construct pET29/BoNT/A-F18, a pUCBHB1/BoNT/A-F18.gamma.
construct is digested with restriction endonucleases that 1) excise
the insert comprising the open reading frame encoding
BoNT/A-F18.gamma.; and 2) enable this insert to be operably-linked
to a pET29 vector (EMD Biosciences-Novagen, Madison, Wis.). This
insert is subcloned using a T4 DNA ligase procedure into a pET29
vector that is digested with appropriate restriction endonucleases
to yield pET29/BoNT/A-F18.gamma.. The ligation mixture is
transformed into chemically competent E. coli DH5.alpha. cells
(Invitrogen, Inc, Carlsbad, Calif.) using a heat shock method,
plated on 1.5% Luria-Bertani agar plates (pH 7.0) containing 50
.mu.g/mL of Kanamycin, and placed in a 37.degree. C. incubator for
overnight growth. Bacteria containing expression constructs are
identified as Kanamycin resistant colonies. Candidate constructs
are isolated using an alkaline lysis plasmid mini-preparation
procedure and analyzed by restriction endonuclease digest mapping
to determine the presence and orientation of the insert. This
cloning strategy yielded a pET29 expression construct comprising
the polynucleotide molecule encoding BoNT/A-F18.gamma.
operably-linked to a carboxyl terminal polyhistidine affinity
binding peptide.
[1040] A similar cloning strategy is used to make pET29 expression
constructs comprising a polynucleotide molecule encoding for
BoNT/B-F18.gamma., BoNT/C1-F18.gamma., BoNT/D-F18.gamma.,
BoNT/E-F18.gamma., BoNT/F-F18.gamma., BoNT/G-F18.gamma.,
TeNT-F18.gamma., BoNT/A-F18.alpha., BoNT/B-F18.alpha.,
BoNT/C1-F18.alpha., BoNT/D-F18.alpha., BoNT/E-F18.alpha.,
BoNT/F-F18.alpha., BoNT/G-F18.alpha., TeNT-F18.alpha.,
BoNT/A-F18.beta., BoNT/B-F18.beta., BoNT/C1-F18.beta.,
BoNT/D-F18.beta., BoNT/E-F18.beta., BoNT/F-F18.beta.,
BoNT/G-F18.beta., or TeNT-F18.beta., as well as the modified
Clostridial toxin indicated above comprising amino acids 109-155 of
SEQ ID NO: 33; amino acids 115-155 of SEQ ID NO: 34; amino acids
166-206 of SEQ ID NO: 35; amino acids 127-172 of SEQ ID NO: 36;
amino acids 148-196 of SEQ ID NO: 37; amino acids 138-183 of SEQ ID
NO: 38; amino acids 26-64 of SEQ ID NO: 33; amino acids 29-67 of
SEQ ID NO: 34; amino acids 83-121 of SEQ ID NO: 35; amino acids
43-80 of SEQ ID NO: 36; amino acids 63-100 of SEQ ID NO: 37; amino
acids 55-91 of SEQ ID NO: 38; amino acids 54-91 of SEQ ID NO: 39;
amino acids 68-105 of SEQ ID NO: 33; amino acids 71-111 of SEQ ID
NO: 34; amino acids 125-162 of SEQ ID NO: 35; amino acids 84-123 of
SEQ ID NO: 36; amino acids 104-144 of SEQ ID NO: 37; amino acids
95-134 of SEQ ID NO: 38; or amino acids 95-134 of SEQ ID NO:
39.
[1041] To construct a BoNT/A-F18.gamma. that replaces the BoNT/A
translocation facilitating domain with another Clostridial toxin
translocation facilitating domain, a translocation facilitating
domain of BoNT/B will be introduced into the BoNT/A-F18.gamma. as
described above using a Splicing by Overlapping ends polymerase
chain reaction (SOE-PCR) procedure, see, e.g., R. M. Horton et al.,
Engineering hybrid genes without the use of restriction enzymes:
gene splicing by overlapping extension, 77(1) Gene 61-68 (1989);
and R. M. Horton, PCR-mediated recombination and mutagenesis.
SOEing together tailor-made genes, 3(2) Mol. Biotechnol. 93-99
(1995). A nucleic acid fragment comprising a region encoding amino
acids 859 to 1097 of BoNT/B (SEQ ID NO: 2) will be operably-linked
by SOE-PCR to replace the region corresponding to the BoNT/A
translocation facilitating domain comprising amino acids 874-1110
of SEQ ID NO: 1 of the BoNT/A-F18.gamma. and will be subcloned into
a pCR2.1 vector using the TOPO.RTM. TA cloning method (Invitrogen,
Inc, Carlsbad, Calif.). The forward and reverse oligonucleotide
primers used for these reactions are designed to include unique
restriction enzyme sites useful for subsequent subcloning steps.
The resulting construct is digested with restriction enzymes that
1) excise the insert containing the entire open reading frame
encoding the modified BoNT/A-F18.gamma.; and 2) enable this insert
to be operably-linked to a pQBI-25A vector (Qbiogene, Inc.,
Carlsbad, Calif.). The resulting restriction fragment will be
purified by the QIAquick Gel Extraction Kit (QIAGEN, Inc.,
Valencia, Calif.), and will be subcloned using a T4 DNA ligase
procedure into a pQBI-25A vector (Qbiogene, Inc., Irvine, Calif.).
This cloning strategy yielded a pQBI-25 expression construct
encoding a BoNT/A-F18.gamma. comprising a BoNT/B translocation
facilitating domain.
[1042] A similar cloning strategy is used to make pQBI-25
expression constructs comprising a polynucleotide molecule encoding
a BoNT/A-F18.gamma. that includes, e.g., a translocation
facilitating domain comprising amino acids 869-1111 of BoNT/C1 of
SEQ ID NO: 3; a translocation facilitating domain comprising amino
acids 865-1098 of BoNT/D of SEQ ID NO: 4; a translocation
facilitating domain comprising amino acids 846-1058 of BoNT/E of
SEQ ID NO: 5; a translocation facilitating domain comprising amino
acids 867-1105 of BoNT/F of SEQ ID NO: 6; a translocation
facilitating domain comprising amino acids 866-1105 of BoNT/G of
SEQ ID NO: 7; or a translocation facilitating domain comprising
amino acids 882-1127 of TeNT of SEQ ID NO: 8.
[1043] Likewise, as well as, a polynucleotide molecule encoding a
Clostridial translocation facilitating domain as described above
can be introduced into a polynucleotide molecule encoding
BoNT/B-F18.gamma., BoNT/C1-F18.gamma., BoNT/D-F18.gamma.,
BoNT/E-F18.gamma., BoNT/F-F18.gamma., BoNT/G-F18.gamma.,
TeNT-F18.gamma., BoNT/A-F18.alpha., BoNT/B-F18.alpha.,
BoNT/C1-F18.alpha., BoNT/D-F18.alpha., BoNT/E-F18.alpha.,
BoNT/F-F18.alpha., BoNT/G-F18.alpha., TeNT-F18.alpha.,
BoNT/A-F18.beta., BoNT/B-F18.beta., BoNT/C1-F18.beta.,
BoNT/D-F18.beta., BoNT/E-F18.beta., BoNT/F-F18.beta.,
BoNT/G-F18.beta., or TeNT-F18.beta., as well as the modified
Clostridial toxin indicated above comprising amino acids 109-155 of
SEQ ID NO: 33; amino acids 115-155 of SEQ ID NO: 34; amino acids
166-206 of SEQ ID NO: 35; amino acids 127-172 of SEQ ID NO: 36;
amino acids 148-196 of SEQ ID NO: 37; amino acids 138-183 of SEQ ID
NO: 38; amino acids 26-64 of SEQ ID NO: 33; amino acids 29-67 of
SEQ ID NO: 34; amino acids 83-121 of SEQ ID NO: 35; amino acids
43-80 of SEQ ID NO: 36; amino acids 63-100 of SEQ ID NO: 37; amino
acids 55-91 of SEQ ID NO: 38; amino acids 54-91 of SEQ ID NO: 39;
amino acids 68-105 of SEQ ID NO: 33; amino acids 71-111 of SEQ ID
NO: 34; amino acids 125-162 of SEQ ID NO: 35; amino acids 84-123 of
SEQ ID NO: 36; amino acids 104-144 of SEQ ID NO: 37; amino acids
95-134 of SEQ ID NO: 38; or amino acids 95-134 of SEQ ID NO:
39.
Example 6
Expression of Modified Clostridial Toxins in a Bacterial Cell
[1044] The following example illustrates a procedure useful for
expressing any of the modified Clostridial toxins disclosed in the
present specification in a bacterial cell.
[1045] An expression construct, such as, e.g., any of the
expression constructs in Examples 1-5, is introduced into
chemically competent E. coli BL21 (DE3) cells (Invitrogen, Inc,
Carlsbad, Calif.) using a heat-shock transformation protocol. The
heat-shock reaction is plated onto 1.5% Luria-Bertani agar plates
(pH 7.0) containing 50 .mu.g/mL of Kanamycin and is placed in a
37.degree. C. incubator for overnight growth. Kanamycin-resistant
colonies of transformed E. coli containing the expression construct
are used to inoculate a baffled flask containing 3.0 mL of PA-0.5 G
media containing 50 .mu.g/mL of Kanamycin which is then placed in a
37.degree. C. incubator, shaking at 250 rpm, for overnight growth.
The resulting overnight starter culture is in turn used to
inoculate a 3 L baffled flask containing ZYP-5052 autoinducing
media containing 50 .mu.g/mL of Kanamycin at a dilution of 1:1000.
Culture volumes ranged from about 600 mL (20% flask volume) to
about 750 mL (25% flask volume). These cultures are grown in a
37.degree. C. incubator shaking at 250 rpm for approximately 5.5
hours and are then transferred to a 16.degree. C. incubator shaking
at 250 rpm for overnight expression. Cells are harvested by
centrifugation (4,000 rpm at 4.degree. C. for 20-30 minutes) and
are used immediately, or stored dry at -80.degree. C. until
needed.
Example 7
Purification and Quantification of Modified Clostridial Toxins
[1046] The following example illustrates methods useful for
purification and quantification of any modified Clostridial toxins
disclosed in the present specification.
[1047] For immobilized metal affinity chromatography (IMAC) protein
purification, E. coli BL21 (DE3) cell pellets used to express a
modified Clostridial toxin, as described in Example 7, are
resuspended in Column Binding Buffer (25 mM N-(2-hydroxyethyl)
piperazine-N'-(2-ethanesulfonic acid) (HEPES), pH 7.8; 500 mM
sodium chloride; 10 mM imidazole; 2.times. Protease Inhibitor
Cocktail Set III (EMD Biosciences-Calbiochem, San Diego Calif.); 5
units/mL of Benzonase (EMD Biosciences-Novagen, Madison, Wis.);
0.1% (v/v) Triton-X.RTM. 100, 4-octylphenol polyethoxylate; 10%
(v/v) glycerol), and then are transferred to a cold Oakridge
centrifuge tube. The cell suspension is sonicated on ice (10-12
pulses of 10 seconds at 40% amplitude with 60 seconds cooling
intervals on a Branson Digital Sonifier) in order to lyse the cells
and then is centrifuged (16,000 rpm at 4.degree. C. for 20 minutes)
to clarify the lysate. An immobilized metal affinity chromatography
column is prepared using a 20 mL Econo-Pac column support (Bio-Rad
Laboratories, Hercules, Calif.) packed with 2.5-5.0 mL of TALON.TM.
SuperFlow Co.sup.2+ affinity resin (BD Biosciences-Clontech, Palo
Alto, Calif.), which is then equilibrated by rinsing with 5 column
volumes of deionized, distilled water, followed by 5 column volumes
of Column Binding Buffer. The clarified lysate is applied slowly to
the equilibrated column by gravity flow (approximately 0.25-0.3
mL/minute). The column is then washed with 5 column volumes of
Column Wash Buffer (N-(2-hydroxyethyl)
piperazine-N'-(2-ethanesulfonic acid) (HEPES), pH 7.8; 500 mM
sodium chloride; 10 mM imidazole; 0.1% (v/v) Triton-X.RTM. 100,
4-octylphenol polyethoxylate; 10% (v/v) glycerol). The modified
Clostridial toxin is eluted with 20-30 mL of Column Elution Buffer
(25 mM N-(2-hydroxyethyl) piperazine-N'-(2-ethanesulfonic acid)
(HEPES), pH 7.8; 500 mM sodium chloride; 500 mM imidazole; 0.1%
(v/v) Triton-X.RTM. 100, 4-octylphenol polyethoxylate; 10% (v/v)
glycerol) and is collected in approximately twelve 1 mL fractions.
The amount of modified Clostridial toxin contained in each elution
fraction is determined by a Bradford dye assay. In this procedure,
20 .mu.L aliquots of each 1.0 mL fraction is combined with 200
.mu.L of Bio-Rad Protein Reagent (Bio-Rad Laboratories, Hercules,
Calif.), diluted 1 to 4 with deionized, distilled water, and then
the intensity of the colorimetric signal is measured using a
spectrophotometer. The five fractions with the strongest signal are
considered the elution peak and are combined together. Total
protein yield is determined by estimating the total protein
concentration of the pooled peak elution fractions using bovine
gamma globulin as a standard (Bio-Rad Laboratories, Hercules,
Calif.).
[1048] For purification of a modified Clostridial toxin using a
FPLC desalting column, a HiPrep.TM. 26/10 size exclusion column
(Amersham Biosciences, Piscataway, N.J.) is pre-equilibrated with
80 mL of 4.degree. C. Column Buffer (50 mM sodium phosphate, pH
6.5). After the column is equilibrated, a modified Clostridial
toxin sample is applied to the size exclusion column with an
isocratic mobile phase of 4.degree. C. Column Buffer and at a flow
rate of 10 mL/minute using a BioLogic DuoFlow chromatography system
(Bio-Rad Laboratories, Hercules, Calif.). The desalted modified
Clostridial toxin sample is collected as a single fraction of
approximately 7-12 mL.
[1049] For purification of a modified Clostridial toxin using a
FPLC ion exchange column, a modified Clostridial toxin sample that
has been desalted following elution from an IMAC column is applied
to a 1 mL Q1.TM. anion exchange column (Bio-Rad Laboratories,
Hercules, Calif.) using a BioLogic DuoFlow chromatography system
(Bio-Rad Laboratories, Hercules, Calif.). The sample is applied to
the column in 4.degree. C. Column Buffer (50 mM sodium phosphate,
pH 6.5) and is eluted by linear gradient with 4.degree. C. Elution
Buffer (50 mM sodium phosphate, 1 M sodium chloride, pH 6.5) as
follows: step 1, 5.0 mL of 5% Elution Buffer at a flow rate of 1
mL/minute; step 2, 20.0 mL of 5-30% Elution Buffer at a flow rate
of 1 mL/minute; step 3, 2.0 mL of 50% Elution Buffer at a flow rate
of 1.0 mL/minute; step 4, 4.0 mL of 100% Elution Buffer at a flow
rate of 1.0 mL/minute; and step 5, 5.0 mL of 0% Elution Buffer at a
flow rate of 1.0 mL/minute. Elution of modified Clostridial toxin
from the column is monitored at 280, 260, and 214 nm, and peaks
absorbing above a minimum threshold (0.01 au) at 280 nm are
collected. Most of the modified Clostridial toxin will elute at a
sodium chloride concentration of approximately 100 to 200 mM.
Average total yields of modified Clostridial toxin will be
determined by a Bradford assay.
[1050] Expression of a modified Clostridial toxin is analyzed by
polyacrylamide gel electrophoresis. Samples purified using the
procedure described above are added to 2.times.LDS Sample Buffer
(Invitrogen, Inc, Carlsbad, Calif.) and are separated by MOPS
polyacrylamide gel electrophoresis using NuPAGE.RTM. Novex 4-12%
Bis-Tris precast polyacrylamide gels (Invitrogen, Inc, Carlsbad,
Calif.) under denaturing, reducing conditions. Gels are stained
with SYPRO.RTM. Ruby (Bio-Rad Laboratories, Hercules, Calif.) and
the separated polypeptides are imaged using a Fluor-S MAX
Multilmager (Bio-Rad Laboratories, Hercules, Calif.) for
quantification of modified Clostridial toxin expression levels. The
size and amount of modified Clostridial toxin is determined by
comparison to MagicMark.TM. protein molecular weight standards
(Invitrogen, Inc, Carlsbad, Calif.).
[1051] Expression of modified Clostridial toxin is also analyzed by
Western blot analysis. Protein samples purified using the procedure
described above are added to 2.times.LDS Sample Buffer (Invitrogen,
Inc, Carlsbad, Calif.) and are separated by MOPS polyacrylamide gel
electrophoresis using NuPAGE.RTM. Novex 4-12% Bis-Tris precast
polyacrylamide gels (Invitrogen, Inc, Carlsbad, Calif.) under
denaturing, reducing conditions. Separated polypeptides are
transferred from the gel onto polyvinylidene fluoride (PVDF)
membranes (Invitrogen, Inc, Carlsbad, Calif.) by Western blotting
using a Trans-Blot.RTM. SD semi-dry electrophoretic transfer cell
apparatus (Bio-Rad Laboratories, Hercules, Calif.). PVDF membranes
are blocked by incubating at room temperature for 2 hours in a
solution containing 25 mM Tris-Buffered Saline (25 mM
2-amino-2-hydroxymethyl-1,3-propanediol hydrochloric acid
(Tris-HCl)(pH 7.4), 137 mM sodium chloride, 2.7 mM potassium
chloride), 0.1% TWEEN-20.RTM., polyoxyethylene (20) sorbitan
monolaureate, 2% bovine serum albumin, 5% nonfat dry milk. Blocked
membranes are incubated at 4.degree. C. for overnight in
Tris-Buffered Saline TWEEN-20.RTM. (25 mM Tris-Buffered Saline,
0.1% TWEEN-20.RTM., polyoxyethylene (20) sorbitan monolaureate)
containing appropriate primary antibodies as a probe. Primary
antibody probed blots are washed three times for 15 minutes each
time in Tris-Buffered Saline TWEEN-20.RTM.. Washed membranes are
incubated at room temperature for 2 hours in Tris-Buffered Saline
TWEEN-20.RTM. containing an appropriate immunoglobulin G antibody
conjugated to horseradish peroxidase as a secondary antibody.
Secondary antibody-probed blots are washed three times for 15
minutes each time in Tris-Buffered Saline TWEEN-20.RTM.. Signal
detection of the labeled modified Clostridial toxin are visualized
using the ECL PIus.TM. Western Blot Detection System (Amersham
Biosciences, Piscataway, N.J.) and are imaged with a Typhoon 9410
Variable Mode Imager (Amersham Biosciences, Piscataway, N.J.) for
quantification of modified Clostridial toxin expression levels.
[1052] Although aspects of the present invention have been
described with reference to the disclosed embodiments, one skilled
in the art will readily appreciate that the specific examples
disclosed are only illustrative of these aspects and in no way
limit the present invention. Various modifications can be made
without departing from the spirit of the present invention.
Sequence CWU 1
1
16211296PRTClostridium botulinum serotype A 1Met Pro Phe Val Asn
Lys Gln Phe Asn Tyr Lys Asp Pro Val Asn Gly1 5 10 15Val Asp Ile Ala
Tyr Ile Lys Ile Pro Asn Ala Gly Gln Met Gln Pro 20 25 30Val Lys Ala
Phe Lys Ile His Asn Lys Ile Trp Val Ile Pro Glu Arg 35 40 45Asp Thr
Phe Thr Asn Pro Glu Glu Gly Asp Leu Asn Pro Pro Pro Glu 50 55 60Ala
Lys Gln Val Pro Val Ser Tyr Tyr Asp Ser Thr Tyr Leu Ser Thr65 70 75
80Asp Asn Glu Lys Asp Asn Tyr Leu Lys Gly Val Thr Lys Leu Phe Glu
85 90 95Arg Ile Tyr Ser Thr Asp Leu Gly Arg Met Leu Leu Thr Ser Ile
Val 100 105 110Arg Gly Ile Pro Phe Trp Gly Gly Ser Thr Ile Asp Thr
Glu Leu Lys 115 120 125Val Ile Asp Thr Asn Cys Ile Asn Val Ile Gln
Pro Asp Gly Ser Tyr 130 135 140Arg Ser Glu Glu Leu Asn Leu Val Ile
Ile Gly Pro Ser Ala Asp Ile145 150 155 160Ile Gln Phe Glu Cys Lys
Ser Phe Gly His Glu Val Leu Asn Leu Thr 165 170 175Arg Asn Gly Tyr
Gly Ser Thr Gln Tyr Ile Arg Phe Ser Pro Asp Phe 180 185 190Thr Phe
Gly Phe Glu Glu Ser Leu Glu Val Asp Thr Asn Pro Leu Leu 195 200
205Gly Ala Gly Lys Phe Ala Thr Asp Pro Ala Val Thr Leu Ala His Glu
210 215 220Leu Ile His Ala Gly His Arg Leu Tyr Gly Ile Ala Ile Asn
Pro Asn225 230 235 240Arg Val Phe Lys Val Asn Thr Asn Ala Tyr Tyr
Glu Met Ser Gly Leu 245 250 255Glu Val Ser Phe Glu Glu Leu Arg Thr
Phe Gly Gly His Asp Ala Lys 260 265 270Phe Ile Asp Ser Leu Gln Glu
Asn Glu Phe Arg Leu Tyr Tyr Tyr Asn 275 280 285Lys Phe Lys Asp Ile
Ala Ser Thr Leu Asn Lys Ala Lys Ser Ile Val 290 295 300Gly Thr Thr
Ala Ser Leu Gln Tyr Met Lys Asn Val Phe Lys Glu Lys305 310 315
320Tyr Leu Leu Ser Glu Asp Thr Ser Gly Lys Phe Ser Val Asp Lys Leu
325 330 335Lys Phe Asp Lys Leu Tyr Lys Met Leu Thr Glu Ile Tyr Thr
Glu Asp 340 345 350Asn Phe Val Lys Phe Phe Lys Val Leu Asn Arg Lys
Thr Tyr Leu Asn 355 360 365Phe Asp Lys Ala Val Phe Lys Ile Asn Ile
Val Pro Lys Val Asn Tyr 370 375 380Thr Ile Tyr Asp Gly Phe Asn Leu
Arg Asn Thr Asn Leu Ala Ala Asn385 390 395 400Phe Asn Gly Gln Asn
Thr Glu Ile Asn Asn Met Asn Phe Thr Lys Leu 405 410 415Lys Asn Phe
Thr Gly Leu Phe Glu Phe Tyr Lys Leu Leu Cys Val Arg 420 425 430Gly
Ile Ile Thr Ser Lys Thr Lys Ser Leu Asp Lys Gly Tyr Asn Lys 435 440
445Ala Leu Asn Asp Leu Cys Ile Lys Val Asn Asn Trp Asp Leu Phe Phe
450 455 460Ser Pro Ser Glu Asp Asn Phe Thr Asn Asp Leu Asn Lys Gly
Glu Glu465 470 475 480Ile Thr Ser Asp Thr Asn Ile Glu Ala Ala Glu
Glu Asn Ile Ser Leu 485 490 495Asp Leu Ile Gln Gln Tyr Tyr Leu Thr
Phe Asn Phe Asp Asn Glu Pro 500 505 510Glu Asn Ile Ser Ile Glu Asn
Leu Ser Ser Asp Ile Ile Gly Gln Leu 515 520 525Glu Leu Met Pro Asn
Ile Glu Arg Phe Pro Asn Gly Lys Lys Tyr Glu 530 535 540Leu Asp Lys
Tyr Thr Met Phe His Tyr Leu Arg Ala Gln Glu Phe Glu545 550 555
560His Gly Lys Ser Arg Ile Ala Leu Thr Asn Ser Val Asn Glu Ala Leu
565 570 575Leu Asn Pro Ser Arg Val Tyr Thr Phe Phe Ser Ser Asp Tyr
Val Lys 580 585 590Lys Val Asn Lys Ala Thr Glu Ala Ala Met Phe Leu
Gly Trp Val Glu 595 600 605Gln Leu Val Tyr Asp Phe Thr Asp Glu Thr
Ser Glu Val Ser Thr Thr 610 615 620Asp Lys Ile Ala Asp Ile Thr Ile
Ile Ile Pro Tyr Ile Gly Pro Ala625 630 635 640Leu Asn Ile Gly Asn
Met Leu Tyr Lys Asp Asp Phe Val Gly Ala Leu 645 650 655Ile Phe Ser
Gly Ala Val Ile Leu Leu Glu Phe Ile Pro Glu Ile Ala 660 665 670Ile
Pro Val Leu Gly Thr Phe Ala Leu Val Ser Tyr Ile Ala Asn Lys 675 680
685Val Leu Thr Val Gln Thr Ile Asp Asn Ala Leu Ser Lys Arg Asn Glu
690 695 700Lys Trp Asp Glu Val Tyr Lys Tyr Ile Val Thr Asn Trp Leu
Ala Lys705 710 715 720Val Asn Thr Gln Ile Asp Leu Ile Arg Lys Lys
Met Lys Glu Ala Leu 725 730 735Glu Asn Gln Ala Glu Ala Thr Lys Ala
Ile Ile Asn Tyr Gln Tyr Asn 740 745 750Gln Tyr Thr Glu Glu Glu Lys
Asn Asn Ile Asn Phe Asn Ile Asp Asp 755 760 765Leu Ser Ser Lys Leu
Asn Glu Ser Ile Asn Lys Ala Met Ile Asn Ile 770 775 780Asn Lys Phe
Leu Asn Gln Cys Ser Val Ser Tyr Leu Met Asn Ser Met785 790 795
800Ile Pro Tyr Gly Val Lys Arg Leu Glu Asp Phe Asp Ala Ser Leu Lys
805 810 815Asp Ala Leu Leu Lys Tyr Ile Tyr Asp Asn Arg Gly Thr Leu
Ile Gly 820 825 830Gln Val Asp Arg Leu Lys Asp Lys Val Asn Asn Thr
Leu Ser Thr Asp 835 840 845Ile Pro Phe Gln Leu Ser Lys Tyr Val Asp
Asn Gln Arg Leu Leu Ser 850 855 860Thr Phe Thr Glu Tyr Ile Lys Asn
Ile Ile Asn Thr Ser Ile Leu Asn865 870 875 880Leu Arg Tyr Glu Ser
Asn His Leu Ile Asp Leu Ser Arg Tyr Ala Ser 885 890 895Lys Ile Asn
Ile Gly Ser Lys Val Asn Phe Asp Pro Ile Asp Lys Asn 900 905 910Gln
Ile Gln Leu Phe Asn Leu Glu Ser Ser Lys Ile Glu Val Ile Leu 915 920
925Lys Asn Ala Ile Val Tyr Asn Ser Met Tyr Glu Asn Phe Ser Thr Ser
930 935 940Phe Trp Ile Arg Ile Pro Lys Tyr Phe Asn Ser Ile Ser Leu
Asn Asn945 950 955 960Glu Tyr Thr Ile Ile Asn Cys Met Glu Asn Asn
Ser Gly Trp Lys Val 965 970 975Ser Leu Asn Tyr Gly Glu Ile Ile Trp
Thr Leu Gln Asp Thr Gln Glu 980 985 990Ile Lys Gln Arg Val Val Phe
Lys Tyr Ser Gln Met Ile Asn Ile Ser 995 1000 1005Asp Tyr Ile Asn
Arg Trp Ile Phe Val Thr Ile Thr Asn Asn Arg Leu 1010 1015 1020Asn
Asn Ser Lys Ile Tyr Ile Asn Gly Arg Leu Ile Asp Gln Lys Pro1025
1030 1035 1040Ile Ser Asn Leu Gly Asn Ile His Ala Ser Asn Asn Ile
Met Phe Lys 1045 1050 1055Leu Asp Gly Cys Arg Asp Thr His Arg Tyr
Ile Trp Ile Lys Tyr Phe 1060 1065 1070Asn Leu Phe Asp Lys Glu Leu
Asn Glu Lys Glu Ile Lys Asp Leu Tyr 1075 1080 1085Asp Asn Gln Ser
Asn Ser Gly Ile Leu Lys Asp Phe Trp Gly Asp Tyr 1090 1095 1100Leu
Gln Tyr Asp Lys Pro Tyr Tyr Met Leu Asn Leu Tyr Asp Pro Asn1105
1110 1115 1120Lys Tyr Val Asp Val Asn Asn Val Gly Ile Arg Gly Tyr
Met Tyr Leu 1125 1130 1135Lys Gly Pro Arg Gly Ser Val Met Thr Thr
Asn Ile Tyr Leu Asn Ser 1140 1145 1150Ser Leu Tyr Arg Gly Thr Lys
Phe Ile Ile Lys Lys Tyr Ala Ser Gly 1155 1160 1165Asn Lys Asp Asn
Ile Val Arg Asn Asn Asp Arg Val Tyr Ile Asn Val 1170 1175 1180Val
Val Lys Asn Lys Glu Tyr Arg Leu Ala Thr Asn Ala Ser Gln Ala1185
1190 1195 1200Gly Val Glu Lys Ile Leu Ser Ala Leu Glu Ile Pro Asp
Val Gly Asn 1205 1210 1215Leu Ser Gln Val Val Val Met Lys Ser Lys
Asn Asp Gln Gly Ile Thr 1220 1225 1230Asn Lys Cys Lys Met Asn Leu
Gln Asp Asn Asn Gly Asn Asp Ile Gly 1235 1240 1245Phe Ile Gly Phe
His Gln Phe Asn Asn Ile Ala Lys Leu Val Ala Ser 1250 1255 1260Asn
Trp Tyr Asn Arg Gln Ile Glu Arg Ser Ser Arg Thr Leu Gly Cys1265
1270 1275 1280Ser Trp Glu Phe Ile Pro Val Asp Asp Gly Trp Gly Glu
Arg Pro Leu 1285 1290 129521291PRTClostridium botulinum serotype B
2Met Pro Val Thr Ile Asn Asn Phe Asn Tyr Asn Asp Pro Ile Asp Asn1 5
10 15Asn Asn Ile Ile Met Met Glu Pro Pro Phe Ala Arg Gly Thr Gly
Arg 20 25 30Tyr Tyr Lys Ala Phe Lys Ile Thr Asp Arg Ile Trp Ile Ile
Pro Glu 35 40 45Arg Tyr Thr Phe Gly Tyr Lys Pro Glu Asp Phe Asn Lys
Ser Ser Gly 50 55 60Ile Phe Asn Arg Asp Val Cys Glu Tyr Tyr Asp Pro
Asp Tyr Leu Asn65 70 75 80Thr Asn Asp Lys Lys Asn Ile Phe Leu Gln
Thr Met Ile Lys Leu Phe 85 90 95Asn Arg Ile Lys Ser Lys Pro Leu Gly
Glu Lys Leu Leu Glu Met Ile 100 105 110Ile Asn Gly Ile Pro Tyr Leu
Gly Asp Arg Arg Val Pro Leu Glu Glu 115 120 125Phe Asn Thr Asn Ile
Ala Ser Val Thr Val Asn Lys Leu Ile Ser Asn 130 135 140Pro Gly Glu
Val Glu Arg Lys Lys Gly Ile Phe Ala Asn Leu Ile Ile145 150 155
160Phe Gly Pro Gly Pro Val Leu Asn Glu Asn Glu Thr Ile Asp Ile Gly
165 170 175Ile Gln Asn His Phe Ala Ser Arg Glu Gly Phe Gly Gly Ile
Met Gln 180 185 190Met Lys Phe Cys Pro Glu Tyr Val Ser Val Phe Asn
Asn Val Gln Glu 195 200 205Asn Lys Gly Ala Ser Ile Phe Asn Arg Arg
Gly Tyr Phe Ser Asp Pro 210 215 220Ala Leu Ile Leu Met His Glu Leu
Ile His Val Leu His Gly Leu Tyr225 230 235 240Gly Ile Lys Val Asp
Asp Leu Pro Ile Val Pro Asn Glu Lys Lys Phe 245 250 255Phe Met Gln
Ser Thr Asp Ala Ile Gln Ala Glu Glu Leu Tyr Thr Phe 260 265 270Gly
Gly Gln Asp Pro Ser Ile Ile Thr Pro Ser Thr Asp Lys Ser Ile 275 280
285Tyr Asp Lys Val Leu Gln Asn Phe Arg Gly Ile Val Asp Arg Leu Asn
290 295 300Lys Val Leu Val Cys Ile Ser Asp Pro Asn Ile Asn Ile Asn
Ile Tyr305 310 315 320Lys Asn Lys Phe Lys Asp Lys Tyr Lys Phe Val
Glu Asp Ser Glu Gly 325 330 335Lys Tyr Ser Ile Asp Val Glu Ser Phe
Asp Lys Leu Tyr Lys Ser Leu 340 345 350Met Phe Gly Phe Thr Glu Thr
Asn Ile Ala Glu Asn Tyr Lys Ile Lys 355 360 365Thr Arg Ala Ser Tyr
Phe Ser Asp Ser Leu Pro Pro Val Lys Ile Lys 370 375 380Asn Leu Leu
Asp Asn Glu Ile Tyr Thr Ile Glu Glu Gly Phe Asn Ile385 390 395
400Ser Asp Lys Asp Met Glu Lys Glu Tyr Arg Gly Gln Asn Lys Ala Ile
405 410 415Asn Lys Gln Ala Tyr Glu Glu Ile Ser Lys Glu His Leu Ala
Val Tyr 420 425 430Lys Ile Gln Met Cys Lys Ser Val Lys Ala Pro Gly
Ile Cys Ile Asp 435 440 445Val Asp Asn Glu Asp Leu Phe Phe Ile Ala
Asp Lys Asn Ser Phe Ser 450 455 460Asp Asp Leu Ser Lys Asn Glu Arg
Ile Glu Tyr Asn Thr Gln Ser Asn465 470 475 480Tyr Ile Glu Asn Asp
Phe Pro Ile Asn Glu Leu Ile Leu Asp Thr Asp 485 490 495Leu Ile Ser
Lys Ile Glu Leu Pro Ser Glu Asn Thr Glu Ser Leu Thr 500 505 510Asp
Phe Asn Val Asp Val Pro Val Tyr Glu Lys Gln Pro Ala Ile Lys 515 520
525Lys Ile Phe Thr Asp Glu Asn Thr Ile Phe Gln Tyr Leu Tyr Ser Gln
530 535 540Thr Phe Pro Leu Asp Ile Arg Asp Ile Ser Leu Thr Ser Ser
Phe Asp545 550 555 560Asp Ala Leu Leu Phe Ser Asn Lys Val Tyr Ser
Phe Phe Ser Met Asp 565 570 575Tyr Ile Lys Thr Ala Asn Lys Val Val
Glu Ala Gly Leu Phe Ala Gly 580 585 590Trp Val Lys Gln Ile Val Asn
Asp Phe Val Ile Glu Ala Asn Lys Ser 595 600 605Asn Thr Met Asp Lys
Ile Ala Asp Ile Ser Leu Ile Val Pro Tyr Ile 610 615 620Gly Leu Ala
Leu Asn Val Gly Asn Glu Thr Ala Lys Gly Asn Phe Glu625 630 635
640Asn Ala Phe Glu Ile Ala Gly Ala Ser Ile Leu Leu Glu Phe Ile Pro
645 650 655Glu Leu Leu Ile Pro Val Val Gly Ala Phe Leu Leu Glu Ser
Tyr Ile 660 665 670Asp Asn Lys Asn Lys Ile Ile Lys Thr Ile Asp Asn
Ala Leu Thr Lys 675 680 685Arg Asn Glu Lys Trp Ser Asp Met Tyr Gly
Leu Ile Val Ala Gln Trp 690 695 700Leu Ser Thr Val Asn Thr Gln Phe
Tyr Thr Ile Lys Glu Gly Met Tyr705 710 715 720Lys Ala Leu Asn Tyr
Gln Ala Gln Ala Leu Glu Glu Ile Ile Lys Tyr 725 730 735Arg Tyr Asn
Ile Tyr Ser Glu Lys Glu Lys Ser Asn Ile Asn Ile Asp 740 745 750Phe
Asn Asp Ile Asn Ser Lys Leu Asn Glu Gly Ile Asn Gln Ala Ile 755 760
765Asp Asn Ile Asn Asn Phe Ile Asn Gly Cys Ser Val Ser Tyr Leu Met
770 775 780Lys Lys Met Ile Pro Leu Ala Val Glu Lys Leu Leu Asp Phe
Asp Asn785 790 795 800Thr Leu Lys Lys Asn Leu Leu Asn Tyr Ile Asp
Glu Asn Lys Leu Tyr 805 810 815Leu Ile Gly Ser Ala Glu Tyr Glu Lys
Ser Lys Val Asn Lys Tyr Leu 820 825 830Lys Thr Ile Met Pro Phe Asp
Leu Ser Ile Tyr Thr Asn Asp Thr Ile 835 840 845Leu Ile Glu Met Phe
Asn Lys Tyr Asn Ser Glu Ile Leu Asn Asn Ile 850 855 860Ile Leu Asn
Leu Arg Tyr Lys Asp Asn Asn Leu Ile Asp Leu Ser Gly865 870 875
880Tyr Gly Ala Lys Val Glu Val Tyr Asp Gly Val Glu Leu Asn Asp Lys
885 890 895Asn Gln Phe Lys Leu Thr Ser Ser Ala Asn Ser Lys Ile Arg
Val Thr 900 905 910Gln Asn Gln Asn Ile Ile Phe Asn Ser Val Phe Leu
Asp Phe Ser Val 915 920 925Ser Phe Trp Ile Arg Ile Pro Lys Tyr Lys
Asn Asp Gly Ile Gln Asn 930 935 940Tyr Ile His Asn Glu Tyr Thr Ile
Ile Asn Cys Met Lys Asn Asn Ser945 950 955 960Gly Trp Lys Ile Ser
Ile Arg Gly Asn Arg Ile Ile Trp Thr Leu Ile 965 970 975Asp Ile Asn
Gly Lys Thr Lys Ser Val Phe Phe Glu Tyr Asn Ile Arg 980 985 990Glu
Asp Ile Ser Glu Tyr Ile Asn Arg Trp Phe Phe Val Thr Ile Thr 995
1000 1005Asn Asn Leu Asn Asn Ala Lys Ile Tyr Ile Asn Gly Lys Leu
Glu Ser 1010 1015 1020Asn Thr Asp Ile Lys Asp Ile Arg Glu Val Ile
Ala Asn Gly Glu Ile1025 1030 1035 1040Ile Phe Lys Leu Asp Gly Asp
Ile Asp Arg Thr Gln Phe Ile Trp Met 1045 1050 1055Lys Tyr Phe Ser
Ile Phe Asn Thr Glu Leu Ser Gln Ser Asn Ile Glu 1060 1065 1070Glu
Arg Tyr Lys Ile Gln Ser Tyr Ser Glu Tyr Leu Lys Asp Phe Trp 1075
1080 1085Gly Asn Pro Leu Met Tyr Asn Lys Glu Tyr Tyr Met Phe Asn
Ala Gly 1090 1095 1100Asn Lys Asn Ser Tyr Ile Lys Leu Lys Lys Asp
Ser Pro Val Gly Glu1105 1110 1115 1120Ile Leu Thr Arg Ser Lys Tyr
Asn Gln Asn Ser Lys Tyr Ile Asn Tyr 1125 1130 1135Arg Asp Leu Tyr
Ile Gly Glu Lys Phe Ile Ile Arg Arg Lys Ser Asn 1140 1145 1150Ser
Gln Ser Ile Asn Asp Asp Ile Val Arg Lys Glu Asp Tyr Ile Tyr
1155 1160 1165Leu Asp Phe Phe Asn Leu Asn Gln Glu Trp Arg Val Tyr
Thr Tyr Lys 1170 1175 1180Tyr Phe Lys Lys Glu Glu Glu Lys Leu Phe
Leu Ala Pro Ile Ser Asp1185 1190 1195 1200Ser Asp Glu Phe Tyr Asn
Thr Ile Gln Ile Lys Glu Tyr Asp Glu Gln 1205 1210 1215Pro Thr Tyr
Ser Cys Gln Leu Leu Phe Lys Lys Asp Glu Glu Ser Thr 1220 1225
1230Asp Glu Ile Gly Leu Ile Gly Ile His Arg Phe Tyr Glu Ser Gly Ile
1235 1240 1245Val Phe Glu Glu Tyr Lys Asp Tyr Phe Cys Ile Ser Lys
Trp Tyr Leu 1250 1255 1260Lys Glu Val Lys Arg Lys Pro Tyr Asn Leu
Lys Leu Gly Cys Asn Trp1265 1270 1275 1280Gln Phe Ile Pro Lys Asp
Glu Gly Trp Thr Glu 1285 129031291PRTClostridium botulinum serotype
C1 3Met Pro Ile Thr Ile Asn Asn Phe Asn Tyr Ser Asp Pro Val Asp
Asn1 5 10 15Lys Asn Ile Leu Tyr Leu Asp Thr His Leu Asn Thr Leu Ala
Asn Glu 20 25 30Pro Glu Lys Ala Phe Arg Ile Thr Gly Asn Ile Trp Val
Ile Pro Asp 35 40 45Arg Phe Ser Arg Asn Ser Asn Pro Asn Leu Asn Lys
Pro Pro Arg Val 50 55 60Thr Ser Pro Lys Ser Gly Tyr Tyr Asp Pro Asn
Tyr Leu Ser Thr Asp65 70 75 80Ser Asp Lys Asp Pro Phe Leu Lys Glu
Ile Ile Lys Leu Phe Lys Arg 85 90 95Ile Asn Ser Arg Glu Ile Gly Glu
Glu Leu Ile Tyr Arg Leu Ser Thr 100 105 110Asp Ile Pro Phe Pro Gly
Asn Asn Asn Thr Pro Ile Asn Thr Phe Asp 115 120 125Phe Asp Val Asp
Phe Asn Ser Val Asp Val Lys Thr Arg Gln Gly Asn 130 135 140Asn Trp
Val Lys Thr Gly Ser Ile Asn Pro Ser Val Ile Ile Thr Gly145 150 155
160Pro Arg Glu Asn Ile Ile Asp Pro Glu Thr Ser Thr Phe Lys Leu Thr
165 170 175Asn Asn Thr Phe Ala Ala Gln Glu Gly Phe Gly Ala Leu Ser
Ile Ile 180 185 190Ser Ile Ser Pro Arg Phe Met Leu Thr Tyr Ser Asn
Ala Thr Asn Asp 195 200 205Val Gly Glu Gly Arg Phe Ser Lys Ser Glu
Phe Cys Met Asp Pro Ile 210 215 220Leu Ile Leu Met His Glu Leu Asn
His Ala Met His Asn Leu Tyr Gly225 230 235 240Ile Ala Ile Pro Asn
Asp Gln Thr Ile Ser Ser Val Thr Ser Asn Ile 245 250 255Phe Tyr Ser
Gln Tyr Asn Val Lys Leu Glu Tyr Ala Glu Ile Tyr Ala 260 265 270Phe
Gly Gly Pro Thr Ile Asp Leu Ile Pro Lys Ser Ala Arg Lys Tyr 275 280
285Phe Glu Glu Lys Ala Leu Asp Tyr Tyr Arg Ser Ile Ala Lys Arg Leu
290 295 300Asn Ser Ile Thr Thr Ala Asn Pro Ser Ser Phe Asn Lys Tyr
Ile Gly305 310 315 320Glu Tyr Lys Gln Lys Leu Ile Arg Lys Tyr Arg
Phe Val Val Glu Ser 325 330 335Ser Gly Glu Val Thr Val Asn Arg Asn
Lys Phe Val Glu Leu Tyr Asn 340 345 350Glu Leu Thr Gln Ile Phe Thr
Glu Phe Asn Tyr Ala Lys Ile Tyr Asn 355 360 365Val Gln Asn Arg Lys
Ile Tyr Leu Ser Asn Val Tyr Thr Pro Val Thr 370 375 380Ala Asn Ile
Leu Asp Asp Asn Val Tyr Asp Ile Gln Asn Gly Phe Asn385 390 395
400Ile Pro Lys Ser Asn Leu Asn Val Leu Phe Met Gly Gln Asn Leu Ser
405 410 415Arg Asn Pro Ala Leu Arg Lys Val Asn Pro Glu Asn Met Leu
Tyr Leu 420 425 430Phe Thr Lys Phe Cys His Lys Ala Ile Asp Gly Arg
Ser Leu Tyr Asn 435 440 445Lys Thr Leu Asp Cys Arg Glu Leu Leu Val
Lys Asn Thr Asp Leu Pro 450 455 460Phe Ile Gly Asp Ile Ser Asp Val
Lys Thr Asp Ile Phe Leu Arg Lys465 470 475 480Asp Ile Asn Glu Glu
Thr Glu Val Ile Tyr Tyr Pro Asp Asn Val Ser 485 490 495Val Asp Gln
Val Ile Leu Ser Lys Asn Thr Ser Glu His Gly Gln Leu 500 505 510Asp
Leu Leu Tyr Pro Ser Ile Asp Ser Glu Ser Glu Ile Leu Pro Gly 515 520
525Glu Asn Gln Val Phe Tyr Asp Asn Arg Thr Gln Asn Val Asp Tyr Leu
530 535 540Asn Ser Tyr Tyr Tyr Leu Glu Ser Gln Lys Leu Ser Asp Asn
Val Glu545 550 555 560Asp Phe Thr Phe Thr Arg Ser Ile Glu Glu Ala
Leu Asp Asn Ser Ala 565 570 575Lys Val Tyr Thr Tyr Phe Pro Thr Leu
Ala Asn Lys Val Asn Ala Gly 580 585 590Val Gln Gly Gly Leu Phe Leu
Met Trp Ala Asn Asp Val Val Glu Asp 595 600 605Phe Thr Thr Asn Ile
Leu Arg Lys Asp Thr Leu Asp Lys Ile Ser Asp 610 615 620Val Ser Ala
Ile Ile Pro Tyr Ile Gly Pro Ala Leu Asn Ile Ser Asn625 630 635
640Ser Val Arg Arg Gly Asn Phe Thr Glu Ala Phe Ala Val Thr Gly Val
645 650 655Thr Ile Leu Leu Glu Ala Phe Pro Glu Phe Thr Ile Pro Ala
Leu Gly 660 665 670Ala Phe Val Ile Tyr Ser Lys Val Gln Glu Arg Asn
Glu Ile Ile Lys 675 680 685Thr Ile Asp Asn Cys Leu Glu Gln Arg Ile
Lys Arg Trp Lys Asp Ser 690 695 700Tyr Glu Trp Met Met Gly Thr Trp
Leu Ser Arg Ile Ile Thr Gln Phe705 710 715 720Asn Asn Ile Ser Tyr
Gln Met Tyr Asp Ser Leu Asn Tyr Gln Ala Gly 725 730 735Ala Ile Lys
Ala Lys Ile Asp Leu Glu Tyr Lys Lys Tyr Ser Gly Ser 740 745 750Asp
Lys Glu Asn Ile Lys Ser Gln Val Glu Asn Leu Lys Asn Ser Leu 755 760
765Asp Val Lys Ile Ser Glu Ala Met Asn Asn Ile Asn Lys Phe Ile Arg
770 775 780Glu Cys Ser Val Thr Tyr Leu Phe Lys Asn Met Leu Pro Lys
Val Ile785 790 795 800Asp Glu Leu Asn Glu Phe Asp Arg Asn Thr Lys
Ala Lys Leu Ile Asn 805 810 815Leu Ile Asp Ser His Asn Ile Ile Leu
Val Gly Glu Val Asp Lys Leu 820 825 830Lys Ala Lys Val Asn Asn Ser
Phe Gln Asn Thr Ile Pro Phe Asn Ile 835 840 845Phe Ser Tyr Thr Asn
Asn Ser Leu Leu Lys Asp Ile Ile Asn Glu Tyr 850 855 860Phe Asn Asn
Ile Asn Asp Ser Lys Ile Leu Ser Leu Gln Asn Arg Lys865 870 875
880Asn Thr Leu Val Asp Thr Ser Gly Tyr Asn Ala Glu Val Ser Glu Glu
885 890 895Gly Asp Val Gln Leu Asn Pro Ile Phe Pro Phe Asp Phe Lys
Leu Gly 900 905 910Ser Ser Gly Glu Asp Arg Gly Lys Val Ile Val Thr
Gln Asn Glu Asn 915 920 925Ile Val Tyr Asn Ser Met Tyr Glu Ser Phe
Ser Ile Ser Phe Trp Ile 930 935 940Arg Ile Asn Lys Trp Val Ser Asn
Leu Pro Gly Tyr Thr Ile Ile Asp945 950 955 960Ser Val Lys Asn Asn
Ser Gly Trp Ser Ile Gly Ile Ile Ser Asn Phe 965 970 975Leu Val Phe
Thr Leu Lys Gln Asn Glu Asp Ser Glu Gln Ser Ile Asn 980 985 990Phe
Ser Tyr Asp Ile Ser Asn Asn Ala Pro Gly Tyr Asn Lys Trp Phe 995
1000 1005Phe Val Thr Val Thr Asn Asn Met Met Gly Asn Met Lys Ile
Tyr Ile 1010 1015 1020Asn Gly Lys Leu Ile Asp Thr Ile Lys Val Lys
Glu Leu Thr Gly Ile1025 1030 1035 1040Asn Phe Ser Lys Thr Ile Thr
Phe Glu Ile Asn Lys Ile Pro Asp Thr 1045 1050 1055Gly Leu Ile Thr
Ser Asp Ser Asp Asn Ile Asn Met Trp Ile Arg Asp 1060 1065 1070Phe
Tyr Ile Phe Ala Lys Glu Leu Asp Gly Lys Asp Ile Asn Ile Leu 1075
1080 1085Phe Asn Ser Leu Gln Tyr Thr Asn Val Val Lys Asp Tyr Trp
Gly Asn 1090 1095 1100Asp Leu Arg Tyr Asn Lys Glu Tyr Tyr Met Val
Asn Ile Asp Tyr Leu1105 1110 1115 1120Asn Arg Tyr Met Tyr Ala Asn
Ser Arg Gln Ile Val Phe Asn Thr Arg 1125 1130 1135Arg Asn Asn Asn
Asp Phe Asn Glu Gly Tyr Lys Ile Ile Ile Lys Arg 1140 1145 1150Ile
Arg Gly Asn Thr Asn Asp Thr Arg Val Arg Gly Gly Asp Ile Leu 1155
1160 1165Tyr Phe Asp Met Thr Ile Asn Asn Lys Ala Tyr Asn Leu Phe
Met Lys 1170 1175 1180Asn Glu Thr Met Tyr Ala Asp Asn His Ser Thr
Glu Asp Ile Tyr Ala1185 1190 1195 1200Ile Gly Leu Arg Glu Gln Thr
Lys Asp Ile Asn Asp Asn Ile Ile Phe 1205 1210 1215Gln Ile Gln Pro
Met Asn Asn Thr Tyr Tyr Tyr Ala Ser Gln Ile Phe 1220 1225 1230Lys
Ser Asn Phe Asn Gly Glu Asn Ile Ser Gly Ile Cys Ser Ile Gly 1235
1240 1245Thr Tyr Arg Phe Arg Leu Gly Gly Asp Trp Tyr Arg His Asn
Tyr Leu 1250 1255 1260Val Pro Thr Val Lys Gln Gly Asn Tyr Ala Ser
Leu Leu Glu Ser Thr1265 1270 1275 1280Ser Thr His Trp Gly Phe Val
Pro Val Ser Glu 1285 129041276PRTClostridium botulinum serotype D
4Met Thr Trp Pro Val Lys Asp Phe Asn Tyr Ser Asp Pro Val Asn Asp1 5
10 15Asn Asp Ile Leu Tyr Leu Arg Ile Pro Gln Asn Lys Leu Ile Thr
Thr 20 25 30Pro Val Lys Ala Phe Met Ile Thr Gln Asn Ile Trp Val Ile
Pro Glu 35 40 45Arg Phe Ser Ser Asp Thr Asn Pro Ser Leu Ser Lys Pro
Pro Arg Pro 50 55 60Thr Ser Lys Tyr Gln Ser Tyr Tyr Asp Pro Ser Tyr
Leu Ser Thr Asp65 70 75 80Glu Gln Lys Asp Thr Phe Leu Lys Gly Ile
Ile Lys Leu Phe Lys Arg 85 90 95Ile Asn Glu Arg Asp Ile Gly Lys Lys
Leu Ile Asn Tyr Leu Val Val 100 105 110Gly Ser Pro Phe Met Gly Asp
Ser Ser Thr Pro Glu Asp Thr Phe Asp 115 120 125Phe Thr Arg His Thr
Thr Asn Ile Ala Val Glu Lys Phe Glu Asn Gly 130 135 140Ser Trp Lys
Val Thr Asn Ile Ile Thr Pro Ser Val Leu Ile Phe Gly145 150 155
160Pro Leu Pro Asn Ile Leu Asp Tyr Thr Ala Ser Leu Thr Leu Gln Gly
165 170 175Gln Gln Ser Asn Pro Ser Phe Glu Gly Phe Gly Thr Leu Ser
Ile Leu 180 185 190Lys Val Ala Pro Glu Phe Leu Leu Thr Phe Ser Asp
Val Thr Ser Asn 195 200 205Gln Ser Ser Ala Val Leu Gly Lys Ser Ile
Phe Cys Met Asp Pro Val 210 215 220Ile Ala Leu Met His Glu Leu Thr
His Ser Leu His Gln Leu Tyr Gly225 230 235 240Ile Asn Ile Pro Ser
Asp Lys Arg Ile Arg Pro Gln Val Ser Glu Gly 245 250 255Phe Phe Ser
Gln Asp Gly Pro Asn Val Gln Phe Glu Glu Leu Tyr Thr 260 265 270Phe
Gly Gly Leu Asp Val Glu Ile Ile Pro Gln Ile Glu Arg Ser Gln 275 280
285Leu Arg Glu Lys Ala Leu Gly His Tyr Lys Asp Ile Ala Lys Arg Leu
290 295 300Asn Asn Ile Asn Lys Thr Ile Pro Ser Ser Trp Ile Ser Asn
Ile Asp305 310 315 320Lys Tyr Lys Lys Ile Phe Ser Glu Lys Tyr Asn
Phe Asp Lys Asp Asn 325 330 335Thr Gly Asn Phe Val Val Asn Ile Asp
Lys Phe Asn Ser Leu Tyr Ser 340 345 350Asp Leu Thr Asn Val Met Ser
Glu Val Val Tyr Ser Ser Gln Tyr Asn 355 360 365Val Lys Asn Arg Thr
His Tyr Phe Ser Arg His Tyr Leu Pro Val Phe 370 375 380Ala Asn Ile
Leu Asp Asp Asn Ile Tyr Thr Ile Arg Asp Gly Phe Asn385 390 395
400Leu Thr Asn Lys Gly Phe Asn Ile Glu Asn Ser Gly Gln Asn Ile Glu
405 410 415Arg Asn Pro Ala Leu Gln Lys Leu Ser Ser Glu Ser Val Val
Asp Leu 420 425 430Phe Thr Lys Val Cys Leu Arg Leu Thr Lys Asn Ser
Arg Asp Asp Ser 435 440 445Thr Cys Ile Lys Val Lys Asn Asn Arg Leu
Pro Tyr Val Ala Asp Lys 450 455 460Asp Ser Ile Ser Gln Glu Ile Phe
Glu Asn Lys Ile Ile Thr Asp Glu465 470 475 480Thr Asn Val Gln Asn
Tyr Ser Asp Lys Phe Ser Leu Asp Glu Ser Ile 485 490 495Leu Asp Gly
Gln Val Pro Ile Asn Pro Glu Ile Val Asp Pro Leu Leu 500 505 510Pro
Asn Val Asn Met Glu Pro Leu Asn Leu Pro Gly Glu Glu Ile Val 515 520
525Phe Tyr Asp Asp Ile Thr Lys Tyr Val Asp Tyr Leu Asn Ser Tyr Tyr
530 535 540Tyr Leu Glu Ser Gln Lys Leu Ser Asn Asn Val Glu Asn Ile
Thr Leu545 550 555 560Thr Thr Ser Val Glu Glu Ala Leu Gly Tyr Ser
Asn Lys Ile Tyr Thr 565 570 575Phe Leu Pro Ser Leu Ala Glu Lys Val
Asn Lys Gly Val Gln Ala Gly 580 585 590Leu Phe Leu Asn Trp Ala Asn
Glu Val Val Glu Asp Phe Thr Thr Asn 595 600 605Ile Met Lys Lys Asp
Thr Leu Asp Lys Ile Ser Asp Val Ser Val Ile 610 615 620Ile Pro Tyr
Ile Gly Pro Ala Leu Asn Ile Gly Asn Ser Ala Leu Arg625 630 635
640Gly Asn Phe Asn Gln Ala Phe Ala Thr Ala Gly Val Ala Phe Leu Leu
645 650 655Glu Gly Phe Pro Glu Phe Thr Ile Pro Ala Leu Gly Val Phe
Thr Phe 660 665 670Tyr Ser Ser Ile Gln Glu Arg Glu Lys Ile Ile Lys
Thr Ile Glu Asn 675 680 685Cys Leu Glu Gln Arg Val Lys Arg Trp Lys
Asp Ser Tyr Gln Trp Met 690 695 700Val Ser Asn Trp Leu Ser Arg Ile
Thr Thr Gln Phe Asn His Ile Asn705 710 715 720Tyr Gln Met Tyr Asp
Ser Leu Ser Tyr Gln Ala Asp Ala Ile Lys Ala 725 730 735Lys Ile Asp
Leu Glu Tyr Lys Lys Tyr Ser Gly Ser Asp Lys Glu Asn 740 745 750Ile
Lys Ser Gln Val Glu Asn Leu Lys Asn Ser Leu Asp Val Lys Ile 755 760
765Ser Glu Ala Met Asn Asn Ile Asn Lys Phe Ile Arg Glu Cys Ser Val
770 775 780Thr Tyr Leu Phe Lys Asn Met Leu Pro Lys Val Ile Asp Glu
Leu Asn785 790 795 800Lys Phe Asp Leu Arg Thr Lys Thr Glu Leu Ile
Asn Leu Ile Asp Ser 805 810 815His Asn Ile Ile Leu Val Gly Glu Val
Asp Arg Leu Lys Ala Lys Val 820 825 830Asn Glu Ser Phe Glu Asn Thr
Met Pro Phe Asn Ile Phe Ser Tyr Thr 835 840 845Asn Asn Ser Leu Leu
Lys Asp Ile Ile Asn Glu Tyr Phe Asn Ser Ile 850 855 860Asn Asp Ser
Lys Ile Leu Ser Leu Gln Asn Lys Lys Asn Ala Leu Val865 870 875
880Asp Thr Ser Gly Tyr Asn Ala Glu Val Arg Val Gly Asp Asn Val Gln
885 890 895Leu Asn Thr Ile Tyr Thr Asn Asp Phe Lys Leu Ser Ser Ser
Gly Asp 900 905 910Lys Ile Ile Val Asn Leu Asn Asn Asn Ile Leu Tyr
Ser Ala Ile Tyr 915 920 925Glu Asn Ser Ser Val Ser Phe Trp Ile Lys
Ile Ser Lys Asp Leu Thr 930 935 940Asn Ser His Asn Glu Tyr Thr Ile
Ile Asn Ser Ile Glu Gln Asn Ser945 950 955 960Gly Trp Lys Leu Cys
Ile Arg Asn Gly Asn Ile Glu Trp Ile Leu Gln 965 970 975Asp Val Asn
Arg Lys Tyr Lys Ser Leu Ile Phe Asp Tyr Ser Glu Ser 980 985 990Leu
Ser His Thr Gly Tyr Thr Asn Lys Trp Phe Phe Val Thr Ile Thr 995
1000 1005Asn Asn Ile Met Gly Tyr Met Lys Leu Tyr Ile Asn Gly Glu
Leu Lys 1010 1015 1020Gln Ser Gln Lys Ile Glu Asp Leu Asp Glu Val
Lys Leu Asp Lys Thr1025 1030
1035 1040Ile Val Phe Gly Ile Asp Glu Asn Ile Asp Glu Asn Gln Met
Leu Trp 1045 1050 1055Ile Arg Asp Phe Asn Ile Phe Ser Lys Glu Leu
Ser Asn Glu Asp Ile 1060 1065 1070Asn Ile Val Tyr Glu Gly Gln Ile
Leu Arg Asn Val Ile Lys Asp Tyr 1075 1080 1085Trp Gly Asn Pro Leu
Lys Phe Asp Thr Glu Tyr Tyr Ile Ile Asn Asp 1090 1095 1100Asn Tyr
Ile Asp Arg Tyr Ile Ala Pro Glu Ser Asn Val Leu Val Leu1105 1110
1115 1120Val Gln Tyr Pro Asp Arg Ser Lys Leu Tyr Thr Gly Asn Pro
Ile Thr 1125 1130 1135Ile Lys Ser Val Ser Asp Lys Asn Pro Tyr Ser
Arg Ile Leu Asn Gly 1140 1145 1150Asp Asn Ile Ile Leu His Met Leu
Tyr Asn Ser Arg Lys Tyr Met Ile 1155 1160 1165Ile Arg Asp Thr Asp
Thr Ile Tyr Ala Thr Gln Gly Gly Glu Cys Ser 1170 1175 1180Gln Asn
Cys Val Tyr Ala Leu Lys Leu Gln Ser Asn Leu Gly Asn Tyr1185 1190
1195 1200Gly Ile Gly Ile Phe Ser Ile Lys Asn Ile Val Ser Lys Asn
Lys Tyr 1205 1210 1215Cys Ser Gln Ile Phe Ser Ser Phe Arg Glu Asn
Thr Met Leu Leu Ala 1220 1225 1230Asp Ile Tyr Lys Pro Trp Arg Phe
Ser Phe Lys Asn Ala Tyr Thr Pro 1235 1240 1245Val Ala Val Thr Asn
Tyr Glu Thr Lys Leu Leu Ser Thr Ser Ser Phe 1250 1255 1260Trp Lys
Phe Ile Ser Arg Asp Pro Gly Trp Val Glu1265 1270
127551252PRTClostridium botulinum serotype E 5Met Pro Lys Ile Asn
Ser Phe Asn Tyr Asn Asp Pro Val Asn Asp Arg1 5 10 15Thr Ile Leu Tyr
Ile Lys Pro Gly Gly Cys Gln Glu Phe Tyr Lys Ser 20 25 30Phe Asn Ile
Met Lys Asn Ile Trp Ile Ile Pro Glu Arg Asn Val Ile 35 40 45Gly Thr
Thr Pro Gln Asp Phe His Pro Pro Thr Ser Leu Lys Asn Gly 50 55 60Asp
Ser Ser Tyr Tyr Asp Pro Asn Tyr Leu Gln Ser Asp Glu Glu Lys65 70 75
80Asp Arg Phe Leu Lys Ile Val Thr Lys Ile Phe Asn Arg Ile Asn Asn
85 90 95Asn Leu Ser Gly Gly Ile Leu Leu Glu Glu Leu Ser Lys Ala Asn
Pro 100 105 110Tyr Leu Gly Asn Asp Asn Thr Pro Asp Asn Gln Phe His
Ile Gly Asp 115 120 125Ala Ser Ala Val Glu Ile Lys Phe Ser Asn Gly
Ser Gln Asp Ile Leu 130 135 140Leu Pro Asn Val Ile Ile Met Gly Ala
Glu Pro Asp Leu Phe Glu Thr145 150 155 160Asn Ser Ser Asn Ile Ser
Leu Arg Asn Asn Tyr Met Pro Ser Asn His 165 170 175Gly Phe Gly Ser
Ile Ala Ile Val Thr Phe Ser Pro Glu Tyr Ser Phe 180 185 190Arg Phe
Asn Asp Asn Ser Met Asn Glu Phe Ile Gln Asp Pro Ala Leu 195 200
205Thr Leu Met His Glu Leu Ile His Ser Leu His Gly Leu Tyr Gly Ala
210 215 220Lys Gly Ile Thr Thr Lys Tyr Thr Ile Thr Gln Lys Gln Asn
Pro Leu225 230 235 240Ile Thr Asn Ile Arg Gly Thr Asn Ile Glu Glu
Phe Leu Thr Phe Gly 245 250 255Gly Thr Asp Leu Asn Ile Ile Thr Ser
Ala Gln Ser Asn Asp Ile Tyr 260 265 270Thr Asn Leu Leu Ala Asp Tyr
Lys Lys Ile Ala Ser Lys Leu Ser Lys 275 280 285Val Gln Val Ser Asn
Pro Leu Leu Asn Pro Tyr Lys Asp Val Phe Glu 290 295 300Ala Lys Tyr
Gly Leu Asp Lys Asp Ala Ser Gly Ile Tyr Ser Val Asn305 310 315
320Ile Asn Lys Phe Asn Asp Ile Phe Lys Lys Leu Tyr Ser Phe Thr Glu
325 330 335Phe Asp Leu Ala Thr Lys Phe Gln Val Lys Cys Arg Gln Thr
Tyr Ile 340 345 350Gly Gln Tyr Lys Tyr Phe Lys Leu Ser Asn Leu Leu
Asn Asp Ser Ile 355 360 365Tyr Asn Ile Ser Glu Gly Tyr Asn Ile Asn
Asn Leu Lys Val Asn Phe 370 375 380Arg Gly Gln Asn Ala Asn Leu Asn
Pro Arg Ile Ile Thr Pro Ile Thr385 390 395 400Gly Arg Gly Leu Val
Lys Lys Ile Ile Arg Phe Cys Lys Asn Ile Val 405 410 415Ser Val Lys
Gly Ile Arg Lys Ser Ile Cys Ile Glu Ile Asn Asn Gly 420 425 430Glu
Leu Phe Phe Val Ala Ser Glu Asn Ser Tyr Asn Asp Asp Asn Ile 435 440
445Asn Thr Pro Lys Glu Ile Asp Asp Thr Val Thr Ser Asn Asn Asn Tyr
450 455 460Glu Asn Asp Leu Asp Gln Val Ile Leu Asn Phe Asn Ser Glu
Ser Ala465 470 475 480Pro Gly Leu Ser Asp Glu Lys Leu Asn Leu Thr
Ile Gln Asn Asp Ala 485 490 495Tyr Ile Pro Lys Tyr Asp Ser Asn Gly
Thr Ser Asp Ile Glu Gln His 500 505 510Asp Val Asn Glu Leu Asn Val
Phe Phe Tyr Leu Asp Ala Gln Lys Val 515 520 525Pro Glu Gly Glu Asn
Asn Val Asn Leu Thr Ser Ser Ile Asp Thr Ala 530 535 540Leu Leu Glu
Gln Pro Lys Ile Tyr Thr Phe Phe Ser Ser Glu Phe Ile545 550 555
560Asn Asn Val Asn Lys Pro Val Gln Ala Ala Leu Phe Val Ser Trp Ile
565 570 575Gln Gln Val Leu Val Asp Phe Thr Thr Glu Ala Asn Gln Lys
Ser Thr 580 585 590Val Asp Lys Ile Ala Asp Ile Ser Ile Val Val Pro
Tyr Ile Gly Leu 595 600 605Ala Leu Asn Ile Gly Asn Glu Ala Gln Lys
Gly Asn Phe Lys Asp Ala 610 615 620Leu Glu Leu Leu Gly Ala Gly Ile
Leu Leu Glu Phe Glu Pro Glu Leu625 630 635 640Leu Ile Pro Thr Ile
Leu Val Phe Thr Ile Lys Ser Phe Leu Gly Ser 645 650 655Ser Asp Asn
Lys Asn Lys Val Ile Lys Ala Ile Asn Asn Ala Leu Lys 660 665 670Glu
Arg Asp Glu Lys Trp Lys Glu Val Tyr Ser Phe Ile Val Ser Asn 675 680
685Trp Met Thr Lys Ile Asn Thr Gln Phe Asn Lys Arg Lys Glu Gln Met
690 695 700Tyr Gln Ala Leu Gln Asn Gln Val Asn Ala Ile Lys Thr Ile
Ile Glu705 710 715 720Ser Lys Tyr Asn Ser Tyr Thr Leu Glu Glu Lys
Asn Glu Leu Thr Asn 725 730 735Lys Tyr Asp Ile Lys Gln Ile Glu Asn
Glu Leu Asn Gln Lys Val Ser 740 745 750Ile Ala Met Asn Asn Ile Asp
Arg Phe Leu Thr Glu Ser Ser Ile Ser 755 760 765Tyr Leu Met Lys Leu
Ile Asn Glu Val Lys Ile Asn Lys Leu Arg Glu 770 775 780Tyr Asp Glu
Asn Val Lys Thr Tyr Leu Leu Asn Tyr Ile Ile Gln His785 790 795
800Gly Ser Ile Leu Gly Glu Ser Gln Gln Glu Leu Asn Ser Met Val Thr
805 810 815Asp Thr Leu Asn Asn Ser Ile Pro Phe Lys Leu Ser Ser Tyr
Thr Asp 820 825 830Asp Lys Ile Leu Ile Ser Tyr Phe Asn Lys Phe Phe
Lys Arg Ile Lys 835 840 845Ser Ser Ser Val Leu Asn Met Arg Tyr Lys
Asn Asp Lys Tyr Val Asp 850 855 860Thr Ser Gly Tyr Asp Ser Asn Ile
Asn Ile Asn Gly Asp Val Tyr Lys865 870 875 880Tyr Pro Thr Asn Lys
Asn Gln Phe Gly Ile Tyr Asn Asp Lys Leu Ser 885 890 895Glu Val Asn
Ile Ser Gln Asn Asp Tyr Ile Ile Tyr Asp Asn Lys Tyr 900 905 910Lys
Asn Phe Ser Ile Ser Phe Trp Val Arg Ile Pro Asn Tyr Asp Asn 915 920
925Lys Ile Val Asn Val Asn Asn Glu Tyr Thr Ile Ile Asn Cys Met Arg
930 935 940Asp Asn Asn Ser Gly Trp Lys Val Ser Leu Asn His Asn Glu
Ile Ile945 950 955 960Trp Thr Leu Gln Asp Asn Ala Gly Ile Asn Gln
Lys Leu Ala Phe Asn 965 970 975Tyr Gly Asn Ala Asn Gly Ile Ser Asp
Tyr Ile Asn Lys Trp Ile Phe 980 985 990Val Thr Ile Thr Asn Asp Arg
Leu Gly Asp Ser Lys Leu Tyr Ile Asn 995 1000 1005Gly Asn Leu Ile
Asp Gln Lys Ser Ile Leu Asn Leu Gly Asn Ile His 1010 1015 1020Val
Ser Asp Asn Ile Leu Phe Lys Ile Val Asn Cys Ser Tyr Thr Arg1025
1030 1035 1040Tyr Ile Gly Ile Arg Tyr Phe Asn Ile Phe Asp Lys Glu
Leu Asp Glu 1045 1050 1055Thr Glu Ile Gln Thr Leu Tyr Ser Asn Glu
Pro Asn Thr Asn Ile Leu 1060 1065 1070Lys Asp Phe Trp Gly Asn Tyr
Leu Leu Tyr Asp Lys Glu Tyr Tyr Leu 1075 1080 1085Leu Asn Val Leu
Lys Pro Asn Asn Phe Ile Asp Arg Arg Lys Asp Ser 1090 1095 1100Thr
Leu Ser Ile Asn Asn Ile Arg Ser Thr Ile Leu Leu Ala Asn Arg1105
1110 1115 1120Leu Tyr Ser Gly Ile Lys Val Lys Ile Gln Arg Val Asn
Asn Ser Ser 1125 1130 1135Thr Asn Asp Asn Leu Val Arg Lys Asn Asp
Gln Val Tyr Ile Asn Phe 1140 1145 1150Val Ala Ser Lys Thr His Leu
Phe Pro Leu Tyr Ala Asp Thr Ala Thr 1155 1160 1165Thr Asn Lys Glu
Lys Thr Ile Lys Ile Ser Ser Ser Gly Asn Arg Phe 1170 1175 1180Asn
Gln Val Val Val Met Asn Ser Val Gly Asn Asn Cys Thr Met Asn1185
1190 1195 1200Phe Lys Asn Asn Asn Gly Asn Asn Ile Gly Leu Leu Gly
Phe Lys Ala 1205 1210 1215Asp Thr Val Val Ala Ser Thr Trp Tyr Tyr
Thr His Met Arg Asp His 1220 1225 1230Thr Asn Ser Asn Gly Cys Phe
Trp Asn Phe Ile Ser Glu Glu His Gly 1235 1240 1245Trp Gln Glu Lys
125061274PRTClostridium botulinum serotype F 6Met Pro Val Ala Ile
Asn Ser Phe Asn Tyr Asn Asp Pro Val Asn Asp1 5 10 15Asp Thr Ile Leu
Tyr Met Gln Ile Pro Tyr Glu Glu Lys Ser Lys Lys 20 25 30Tyr Tyr Lys
Ala Phe Glu Ile Met Arg Asn Val Trp Ile Ile Pro Glu 35 40 45Arg Asn
Thr Ile Gly Thr Asn Pro Ser Asp Phe Asp Pro Pro Ala Ser 50 55 60Leu
Lys Asn Gly Ser Ser Ala Tyr Tyr Asp Pro Asn Tyr Leu Thr Thr65 70 75
80Asp Ala Glu Lys Asp Arg Tyr Leu Lys Thr Thr Ile Lys Leu Phe Lys
85 90 95Arg Ile Asn Ser Asn Pro Ala Gly Lys Val Leu Leu Gln Glu Ile
Ser 100 105 110Tyr Ala Lys Pro Tyr Leu Gly Asn Asp His Thr Pro Ile
Asp Glu Phe 115 120 125Ser Pro Val Thr Arg Thr Thr Ser Val Asn Ile
Lys Leu Ser Thr Asn 130 135 140Val Glu Ser Ser Met Leu Leu Asn Leu
Leu Val Leu Gly Ala Gly Pro145 150 155 160Asp Ile Phe Glu Ser Cys
Cys Tyr Pro Val Arg Lys Leu Ile Asp Pro 165 170 175Asp Val Val Tyr
Asp Pro Ser Asn Tyr Gly Phe Gly Ser Ile Asn Ile 180 185 190Val Thr
Phe Ser Pro Glu Tyr Glu Tyr Thr Phe Asn Asp Ile Ser Gly 195 200
205Gly His Asn Ser Ser Thr Glu Ser Phe Ile Ala Asp Pro Ala Ile Ser
210 215 220Leu Ala His Glu Leu Ile His Ala Leu His Gly Leu Tyr Gly
Ala Arg225 230 235 240Gly Val Thr Tyr Glu Glu Thr Ile Glu Val Lys
Gln Ala Pro Leu Met 245 250 255Ile Ala Glu Lys Pro Ile Arg Leu Glu
Glu Phe Leu Thr Phe Gly Gly 260 265 270Gln Asp Leu Asn Ile Ile Thr
Ser Ala Met Lys Glu Lys Ile Tyr Asn 275 280 285Asn Leu Leu Ala Asn
Tyr Glu Lys Ile Ala Thr Arg Leu Ser Glu Val 290 295 300Asn Ser Ala
Pro Pro Glu Tyr Asp Ile Asn Glu Tyr Lys Asp Tyr Phe305 310 315
320Gln Trp Lys Tyr Gly Leu Asp Lys Asn Ala Asp Gly Ser Tyr Thr Val
325 330 335Asn Glu Asn Lys Phe Asn Glu Ile Tyr Lys Lys Leu Tyr Ser
Phe Thr 340 345 350Glu Ser Asp Leu Ala Asn Lys Phe Lys Val Lys Cys
Arg Asn Thr Tyr 355 360 365Phe Ile Lys Tyr Glu Phe Leu Lys Val Pro
Asn Leu Leu Asp Asp Asp 370 375 380Ile Tyr Thr Val Ser Glu Gly Phe
Asn Ile Gly Asn Leu Ala Val Asn385 390 395 400Asn Arg Gly Gln Ser
Ile Lys Leu Asn Pro Lys Ile Ile Asp Ser Ile 405 410 415Pro Asp Lys
Gly Leu Val Glu Lys Ile Val Lys Phe Cys Lys Ser Val 420 425 430Ile
Pro Arg Lys Gly Thr Lys Ala Pro Pro Arg Leu Cys Ile Arg Val 435 440
445Asn Asn Ser Glu Leu Phe Phe Val Ala Ser Glu Ser Ser Tyr Asn Glu
450 455 460Asn Asp Ile Asn Thr Pro Lys Glu Ile Asp Asp Thr Thr Asn
Leu Asn465 470 475 480Asn Asn Tyr Arg Asn Asn Leu Asp Glu Val Ile
Leu Asp Tyr Asn Ser 485 490 495Gln Thr Ile Pro Gln Ile Ser Asn Arg
Thr Leu Asn Thr Leu Val Gln 500 505 510Asp Asn Ser Tyr Val Pro Arg
Tyr Asp Ser Asn Gly Thr Ser Glu Ile 515 520 525Glu Glu Tyr Asp Val
Val Asp Phe Asn Val Phe Phe Tyr Leu His Ala 530 535 540Gln Lys Val
Pro Glu Gly Glu Thr Asn Ile Ser Leu Thr Ser Ser Ile545 550 555
560Asp Thr Ala Leu Leu Glu Glu Ser Lys Asp Ile Phe Phe Ser Ser Glu
565 570 575Phe Ile Asp Thr Ile Asn Lys Pro Val Asn Ala Ala Leu Phe
Ile Asp 580 585 590Trp Ile Ser Lys Val Ile Arg Asp Phe Thr Thr Glu
Ala Thr Gln Lys 595 600 605Ser Thr Val Asp Lys Ile Ala Asp Ile Ser
Leu Ile Val Pro Tyr Val 610 615 620Gly Leu Ala Leu Asn Ile Ile Ile
Glu Ala Glu Lys Gly Asn Phe Glu625 630 635 640Glu Ala Phe Glu Leu
Leu Gly Val Gly Ile Leu Leu Glu Phe Val Pro 645 650 655Glu Leu Thr
Ile Pro Val Ile Leu Val Phe Thr Ile Lys Ser Tyr Ile 660 665 670Asp
Ser Tyr Glu Asn Lys Asn Lys Ala Ile Lys Ala Ile Asn Asn Ser 675 680
685Leu Ile Glu Arg Glu Ala Lys Trp Lys Glu Ile Tyr Ser Trp Ile Val
690 695 700Ser Asn Trp Leu Thr Arg Ile Asn Thr Gln Phe Asn Lys Arg
Lys Glu705 710 715 720Gln Met Tyr Gln Ala Leu Gln Asn Gln Val Asp
Ala Ile Lys Thr Ala 725 730 735Ile Glu Tyr Lys Tyr Asn Asn Tyr Thr
Ser Asp Glu Lys Asn Arg Leu 740 745 750Glu Ser Glu Tyr Asn Ile Asn
Asn Ile Glu Glu Glu Leu Asn Lys Lys 755 760 765Val Ser Leu Ala Met
Lys Asn Ile Glu Arg Phe Met Thr Glu Ser Ser 770 775 780Ile Ser Tyr
Leu Met Lys Leu Ile Asn Glu Ala Lys Val Gly Lys Leu785 790 795
800Lys Lys Tyr Asp Asn His Val Lys Ser Asp Leu Leu Asn Tyr Ile Leu
805 810 815Asp His Arg Ser Ile Leu Gly Glu Gln Thr Asn Glu Leu Ser
Asp Leu 820 825 830Val Thr Ser Thr Leu Asn Ser Ser Ile Pro Phe Glu
Leu Ser Ser Tyr 835 840 845Thr Asn Asp Lys Ile Leu Ile Ile Tyr Phe
Asn Arg Leu Tyr Lys Lys 850 855 860Ile Lys Asp Ser Ser Ile Leu Asp
Met Arg Tyr Glu Asn Asn Lys Phe865 870 875 880Ile Asp Ile Ser Gly
Tyr Gly Ser Asn Ile Ser Ile Asn Gly Asn Val 885 890 895Tyr Ile Tyr
Ser Thr Asn Arg Asn Gln Phe Gly Ile Tyr Asn Ser Arg 900 905 910Leu
Ser Glu Val Asn Ile Ala Gln Asn Asn Asp Ile Ile Tyr Asn Ser 915 920
925Arg Tyr Gln Asn Phe Ser Ile Ser Phe Trp Val Arg Ile Pro Lys His
930 935 940Tyr Lys Pro Met Asn His Asn Arg Glu Tyr Thr Ile Ile Asn
Cys Met945 950 955 960Gly Asn Asn Asn Ser Gly Trp Lys Ile Ser Leu
Arg Thr Val Arg Asp
965 970 975Cys Glu Ile Ile Trp Thr Leu Gln Asp Thr Ser Gly Asn Lys
Glu Asn 980 985 990Leu Ile Phe Arg Tyr Glu Glu Leu Asn Arg Ile Ser
Asn Tyr Ile Asn 995 1000 1005Lys Trp Ile Phe Val Thr Ile Thr Asn
Asn Arg Leu Gly Asn Ser Arg 1010 1015 1020Ile Tyr Ile Asn Gly Asn
Leu Ile Val Glu Lys Ser Ile Ser Asn Leu1025 1030 1035 1040Gly Asp
Ile His Val Ser Asp Asn Ile Leu Phe Lys Ile Val Gly Cys 1045 1050
1055Asp Asp Glu Thr Tyr Val Gly Ile Arg Tyr Phe Lys Val Phe Asn Thr
1060 1065 1070Glu Leu Asp Lys Thr Glu Ile Glu Thr Leu Tyr Ser Asn
Glu Pro Asp 1075 1080 1085Pro Ser Ile Leu Lys Asn Tyr Trp Gly Asn
Tyr Leu Leu Tyr Asn Lys 1090 1095 1100Lys Tyr Tyr Leu Phe Asn Leu
Leu Arg Lys Asp Lys Tyr Ile Thr Leu1105 1110 1115 1120Asn Ser Gly
Ile Leu Asn Ile Asn Gln Gln Arg Gly Val Thr Glu Gly 1125 1130
1135Ser Val Phe Leu Asn Tyr Lys Leu Tyr Glu Gly Val Glu Val Ile Ile
1140 1145 1150Arg Lys Asn Gly Pro Ile Asp Ile Ser Asn Thr Asp Asn
Phe Val Arg 1155 1160 1165Lys Asn Asp Leu Ala Tyr Ile Asn Val Val
Asp Arg Gly Val Glu Tyr 1170 1175 1180Arg Leu Tyr Ala Asp Thr Lys
Ser Glu Lys Glu Lys Ile Ile Arg Thr1185 1190 1195 1200Ser Asn Leu
Asn Asp Ser Leu Gly Gln Ile Ile Val Met Asp Ser Ile 1205 1210
1215Gly Asn Asn Cys Thr Met Asn Phe Gln Asn Asn Asn Gly Ser Asn Ile
1220 1225 1230Gly Leu Leu Gly Phe His Ser Asn Asn Leu Val Ala Ser
Ser Trp Tyr 1235 1240 1245Tyr Asn Asn Ile Arg Arg Asn Thr Ser Ser
Asn Gly Cys Phe Trp Ser 1250 1255 1260Ser Ile Ser Lys Glu Asn Gly
Trp Lys Glu1265 127071297PRTClostridium botulinum serotype G 7Met
Pro Val Asn Ile Lys Asn Phe Asn Tyr Asn Asp Pro Ile Asn Asn1 5 10
15Asp Asp Ile Ile Met Met Glu Pro Phe Asn Asp Pro Gly Pro Gly Thr
20 25 30Tyr Tyr Lys Ala Phe Arg Ile Ile Asp Arg Ile Trp Ile Val Pro
Glu 35 40 45Arg Phe Thr Tyr Gly Phe Gln Pro Asp Gln Phe Asn Ala Ser
Thr Gly 50 55 60Val Phe Ser Lys Asp Val Tyr Glu Tyr Tyr Asp Pro Thr
Tyr Leu Lys65 70 75 80Thr Asp Ala Glu Lys Asp Lys Phe Leu Lys Thr
Met Ile Lys Leu Phe 85 90 95Asn Arg Ile Asn Ser Lys Pro Ser Gly Gln
Arg Leu Leu Asp Met Ile 100 105 110Val Asp Ala Ile Pro Tyr Leu Gly
Asn Ala Ser Thr Pro Pro Asp Lys 115 120 125Phe Ala Ala Asn Val Ala
Asn Val Ser Ile Asn Lys Lys Ile Ile Gln 130 135 140Pro Gly Ala Glu
Asp Gln Ile Lys Gly Leu Met Thr Asn Leu Ile Ile145 150 155 160Phe
Gly Pro Gly Pro Val Leu Ser Asp Asn Phe Thr Asp Ser Met Ile 165 170
175Met Asn Gly His Ser Pro Ile Ser Glu Gly Phe Gly Ala Arg Met Met
180 185 190Ile Arg Phe Cys Pro Ser Cys Leu Asn Val Phe Asn Asn Val
Gln Glu 195 200 205Asn Lys Asp Thr Ser Ile Phe Ser Arg Arg Ala Tyr
Phe Ala Asp Pro 210 215 220Ala Leu Thr Leu Met His Glu Leu Ile His
Val Leu His Gly Leu Tyr225 230 235 240Gly Ile Lys Ile Ser Asn Leu
Pro Ile Thr Pro Asn Thr Lys Glu Phe 245 250 255Phe Met Gln His Ser
Asp Pro Val Gln Ala Glu Glu Leu Tyr Thr Phe 260 265 270Gly Gly His
Asp Pro Ser Val Ile Ser Pro Ser Thr Asp Met Asn Ile 275 280 285Tyr
Asn Lys Ala Leu Gln Asn Phe Gln Asp Ile Ala Asn Arg Leu Asn 290 295
300Ile Val Ser Ser Ala Gln Gly Ser Gly Ile Asp Ile Ser Leu Tyr
Lys305 310 315 320Gln Ile Tyr Lys Asn Lys Tyr Asp Phe Val Glu Asp
Pro Asn Gly Lys 325 330 335Tyr Ser Val Asp Lys Asp Lys Phe Asp Lys
Leu Tyr Lys Ala Leu Met 340 345 350Phe Gly Phe Thr Glu Thr Asn Leu
Ala Gly Glu Tyr Gly Ile Lys Thr 355 360 365Arg Tyr Ser Tyr Phe Ser
Glu Tyr Leu Pro Pro Ile Lys Thr Glu Lys 370 375 380Leu Leu Asp Asn
Thr Ile Tyr Thr Gln Asn Glu Gly Phe Asn Ile Ala385 390 395 400Ser
Lys Asn Leu Lys Thr Glu Phe Asn Gly Gln Asn Lys Ala Val Asn 405 410
415Lys Glu Ala Tyr Glu Glu Ile Ser Leu Glu His Leu Val Ile Tyr Arg
420 425 430Ile Ala Met Cys Lys Pro Val Met Tyr Lys Asn Thr Gly Lys
Ser Glu 435 440 445Gln Cys Ile Ile Val Asn Asn Glu Asp Leu Phe Phe
Ile Ala Asn Lys 450 455 460Asp Ser Phe Ser Lys Asp Leu Ala Lys Ala
Glu Thr Ile Ala Tyr Asn465 470 475 480Thr Gln Asn Asn Thr Ile Glu
Asn Asn Phe Ser Ile Asp Gln Leu Ile 485 490 495Leu Asp Asn Asp Leu
Ser Ser Gly Ile Asp Leu Pro Asn Glu Asn Thr 500 505 510Glu Pro Phe
Thr Asn Phe Asp Asp Ile Asp Ile Pro Val Tyr Ile Lys 515 520 525Gln
Ser Ala Leu Lys Lys Ile Phe Val Asp Gly Asp Ser Leu Phe Glu 530 535
540Tyr Leu His Ala Gln Thr Phe Pro Ser Asn Ile Glu Asn Leu Gln
Leu545 550 555 560Thr Asn Ser Leu Asn Asp Ala Leu Arg Asn Asn Asn
Lys Val Tyr Thr 565 570 575Phe Phe Ser Thr Asn Leu Val Glu Lys Ala
Asn Thr Val Val Gly Ala 580 585 590Ser Leu Phe Val Asn Trp Val Lys
Gly Val Ile Asp Asp Phe Thr Ser 595 600 605Glu Ser Thr Gln Lys Ser
Thr Ile Asp Lys Val Ser Asp Val Ser Ile 610 615 620Ile Ile Pro Tyr
Ile Gly Pro Ala Leu Asn Val Gly Asn Glu Thr Ala625 630 635 640Lys
Glu Asn Phe Lys Asn Ala Phe Glu Ile Gly Gly Ala Ala Ile Leu 645 650
655Met Glu Phe Ile Pro Glu Leu Ile Val Pro Ile Val Gly Phe Phe Thr
660 665 670Leu Glu Ser Tyr Val Gly Asn Lys Gly His Ile Ile Met Thr
Ile Ser 675 680 685Asn Ala Leu Lys Lys Arg Asp Gln Lys Trp Thr Asp
Met Tyr Gly Leu 690 695 700Ile Val Ser Gln Trp Leu Ser Thr Val Asn
Thr Gln Phe Tyr Thr Ile705 710 715 720Lys Glu Arg Met Tyr Asn Ala
Leu Asn Asn Gln Ser Gln Ala Ile Glu 725 730 735Lys Ile Ile Glu Asp
Gln Tyr Asn Arg Tyr Ser Glu Glu Asp Lys Met 740 745 750Asn Ile Asn
Ile Asp Phe Asn Asp Ile Asp Phe Lys Leu Asn Gln Ser 755 760 765Ile
Asn Leu Ala Ile Asn Asn Ile Asp Asp Phe Ile Asn Gln Cys Ser 770 775
780Ile Ser Tyr Leu Met Asn Arg Met Ile Pro Leu Ala Val Lys Lys
Leu785 790 795 800Lys Asp Phe Asp Asp Asn Leu Lys Arg Asp Leu Leu
Glu Tyr Ile Asp 805 810 815Thr Asn Glu Leu Tyr Leu Leu Asp Glu Val
Asn Ile Leu Lys Ser Lys 820 825 830Val Asn Arg His Leu Lys Asp Ser
Ile Pro Phe Asp Leu Ser Leu Tyr 835 840 845Thr Lys Asp Thr Ile Leu
Ile Gln Val Phe Asn Asn Tyr Ile Ser Asn 850 855 860Ile Ser Ser Asn
Ala Ile Leu Ser Leu Ser Tyr Arg Gly Gly Arg Leu865 870 875 880Ile
Asp Ser Ser Gly Tyr Gly Ala Thr Met Asn Val Gly Ser Asp Val 885 890
895Ile Phe Asn Asp Ile Gly Asn Gly Gln Phe Lys Leu Asn Asn Ser Glu
900 905 910Asn Ser Asn Ile Thr Ala His Gln Ser Lys Phe Val Val Tyr
Asp Ser 915 920 925Met Phe Asp Asn Phe Ser Ile Asn Phe Trp Val Arg
Thr Pro Lys Tyr 930 935 940Asn Asn Asn Asp Ile Gln Thr Tyr Leu Gln
Asn Glu Tyr Thr Ile Ile945 950 955 960Ser Cys Ile Lys Asn Asp Ser
Gly Trp Lys Val Ser Ile Lys Gly Asn 965 970 975Arg Ile Ile Trp Thr
Leu Ile Asp Val Asn Ala Lys Ser Lys Ser Ile 980 985 990Phe Phe Glu
Tyr Ser Ile Lys Asp Asn Ile Ser Asp Tyr Ile Asn Lys 995 1000
1005Trp Phe Ser Ile Thr Ile Thr Asn Asp Arg Leu Gly Asn Ala Asn Ile
1010 1015 1020Tyr Ile Asn Gly Ser Leu Lys Lys Ser Glu Lys Ile Leu
Asn Leu Asp1025 1030 1035 1040Arg Ile Asn Ser Ser Asn Asp Ile Asp
Phe Lys Leu Ile Asn Cys Thr 1045 1050 1055Asp Thr Thr Lys Phe Val
Trp Ile Lys Asp Phe Asn Ile Phe Gly Arg 1060 1065 1070Glu Leu Asn
Ala Thr Glu Val Ser Ser Leu Tyr Trp Ile Gln Ser Ser 1075 1080
1085Thr Asn Thr Leu Lys Asp Phe Trp Gly Asn Pro Leu Arg Tyr Asp Thr
1090 1095 1100Gln Tyr Tyr Leu Phe Asn Gln Gly Met Gln Asn Ile Tyr
Ile Lys Tyr1105 1110 1115 1120Phe Ser Lys Ala Ser Met Gly Glu Thr
Ala Pro Arg Thr Asn Phe Asn 1125 1130 1135Asn Ala Ala Ile Asn Tyr
Gln Asn Leu Tyr Leu Gly Leu Arg Phe Ile 1140 1145 1150Ile Lys Lys
Ala Ser Asn Ser Arg Asn Ile Asn Asn Asp Asn Ile Val 1155 1160
1165Arg Glu Gly Asp Tyr Ile Tyr Leu Asn Ile Asp Asn Ile Ser Asp Glu
1170 1175 1180Ser Tyr Arg Val Tyr Val Leu Val Asn Ser Lys Glu Ile
Gln Thr Gln1185 1190 1195 1200Leu Phe Leu Ala Pro Ile Asn Asp Asp
Pro Thr Phe Tyr Asp Val Leu 1205 1210 1215Gln Ile Lys Lys Tyr Tyr
Glu Lys Thr Thr Tyr Asn Cys Gln Ile Leu 1220 1225 1230Cys Glu Lys
Asp Thr Lys Thr Phe Gly Leu Phe Gly Ile Gly Lys Phe 1235 1240
1245Val Lys Asp Tyr Gly Tyr Val Trp Asp Thr Tyr Asp Asn Tyr Phe Cys
1250 1255 1260Ile Ser Gln Trp Tyr Leu Arg Arg Ile Ser Glu Asn Ile
Asn Lys Leu1265 1270 1275 1280Arg Leu Gly Cys Asn Trp Gln Phe Ile
Pro Val Asp Glu Gly Trp Thr 1285 1290 1295Glu81315PRTClostridium
teteni 8Met Pro Ile Thr Ile Asn Asn Phe Arg Tyr Ser Asp Pro Val Asn
Asn1 5 10 15Asp Thr Ile Ile Met Met Glu Pro Pro Tyr Cys Lys Gly Leu
Asp Ile 20 25 30Tyr Tyr Lys Ala Phe Lys Ile Thr Asp Arg Ile Trp Ile
Val Pro Glu 35 40 45Arg Tyr Glu Phe Gly Thr Lys Pro Glu Asp Phe Asn
Pro Pro Ser Ser 50 55 60Leu Ile Glu Gly Ala Ser Glu Tyr Tyr Asp Pro
Asn Tyr Leu Arg Thr65 70 75 80Asp Ser Asp Lys Asp Arg Phe Leu Gln
Thr Met Val Lys Leu Phe Asn 85 90 95Arg Ile Lys Asn Asn Val Ala Gly
Glu Ala Leu Leu Asp Lys Ile Ile 100 105 110Asn Ala Ile Pro Tyr Leu
Gly Asn Ser Tyr Ser Leu Leu Asp Lys Phe 115 120 125Asp Thr Asn Ser
Asn Ser Val Ser Phe Asn Leu Leu Glu Gln Asp Pro 130 135 140Ser Gly
Ala Thr Thr Lys Ser Ala Met Leu Thr Asn Leu Ile Ile Phe145 150 155
160Gly Pro Gly Pro Val Leu Asn Lys Asn Glu Val Arg Gly Ile Val Leu
165 170 175Arg Val Asp Asn Lys Asn Tyr Phe Pro Cys Arg Asp Gly Phe
Gly Ser 180 185 190Ile Met Gln Met Ala Phe Cys Pro Glu Tyr Val Pro
Thr Phe Asp Asn 195 200 205Val Ile Glu Asn Ile Thr Ser Leu Thr Ile
Gly Lys Ser Lys Tyr Phe 210 215 220Gln Asp Pro Ala Leu Leu Leu Met
His Glu Leu Ile His Val Leu His225 230 235 240Gly Leu Tyr Gly Met
Gln Val Ser Ser His Glu Ile Ile Pro Ser Lys 245 250 255Gln Glu Ile
Tyr Met Gln His Thr Tyr Pro Ile Ser Ala Glu Glu Leu 260 265 270Phe
Thr Phe Gly Gly Gln Asp Ala Asn Leu Ile Ser Ile Asp Ile Lys 275 280
285Asn Asp Leu Tyr Glu Lys Thr Leu Asn Asp Tyr Lys Ala Ile Ala Asn
290 295 300Lys Leu Ser Gln Val Thr Ser Cys Asn Asp Pro Asn Ile Asp
Ile Asp305 310 315 320Ser Tyr Lys Gln Ile Tyr Gln Gln Lys Tyr Gln
Phe Asp Lys Asp Ser 325 330 335Asn Gly Gln Tyr Ile Val Asn Glu Asp
Lys Phe Gln Ile Leu Tyr Asn 340 345 350Ser Ile Met Tyr Gly Phe Thr
Glu Ile Glu Leu Gly Lys Lys Phe Asn 355 360 365Ile Lys Thr Arg Leu
Ser Tyr Phe Ser Met Asn His Asp Pro Val Lys 370 375 380Ile Pro Asn
Leu Leu Asp Asp Thr Ile Tyr Asn Asp Thr Glu Gly Phe385 390 395
400Asn Ile Glu Ser Lys Asp Leu Lys Ser Glu Tyr Lys Gly Gln Asn Met
405 410 415Arg Val Asn Thr Asn Ala Phe Arg Asn Val Asp Gly Ser Gly
Leu Val 420 425 430Ser Lys Leu Ile Gly Leu Cys Lys Lys Ile Ile Pro
Pro Thr Asn Ile 435 440 445Arg Glu Asn Leu Tyr Asn Arg Thr Ala Ser
Leu Thr Asp Leu Gly Gly 450 455 460Glu Leu Cys Ile Lys Ile Lys Asn
Glu Asp Leu Thr Phe Ile Ala Glu465 470 475 480Lys Asn Ser Phe Ser
Glu Glu Pro Phe Gln Asp Glu Ile Val Ser Tyr 485 490 495Asn Thr Lys
Asn Lys Pro Leu Asn Phe Asn Tyr Ser Leu Asp Lys Ile 500 505 510Ile
Val Asp Tyr Asn Leu Gln Ser Lys Ile Thr Leu Pro Asn Asp Arg 515 520
525Thr Thr Pro Val Thr Lys Gly Ile Pro Tyr Ala Pro Glu Tyr Lys Ser
530 535 540Asn Ala Ala Ser Thr Ile Glu Ile His Asn Ile Asp Asp Asn
Thr Ile545 550 555 560Tyr Gln Tyr Leu Tyr Ala Gln Lys Ser Pro Thr
Thr Leu Gln Arg Ile 565 570 575Thr Met Thr Asn Ser Val Asp Asp Ala
Leu Ile Asn Ser Thr Lys Ile 580 585 590Tyr Ser Tyr Phe Pro Ser Val
Ile Ser Lys Val Asn Gln Gly Ala Gln 595 600 605Gly Ile Leu Phe Leu
Gln Trp Val Arg Asp Ile Ile Asp Asp Phe Thr 610 615 620Asn Glu Ser
Ser Gln Lys Thr Thr Ile Asp Lys Ile Ser Asp Val Ser625 630 635
640Thr Ile Val Pro Tyr Ile Gly Pro Ala Leu Asn Ile Val Lys Gln Gly
645 650 655Tyr Glu Gly Asn Phe Ile Gly Ala Leu Glu Thr Thr Gly Val
Val Leu 660 665 670Leu Leu Glu Tyr Ile Pro Glu Ile Thr Leu Pro Val
Ile Ala Ala Leu 675 680 685Ser Ile Ala Glu Ser Ser Thr Gln Lys Glu
Lys Ile Ile Lys Thr Ile 690 695 700Asp Asn Phe Leu Glu Lys Arg Tyr
Glu Lys Trp Ile Glu Val Tyr Lys705 710 715 720Leu Val Lys Ala Lys
Trp Leu Gly Thr Val Asn Thr Gln Phe Gln Lys 725 730 735Arg Ser Tyr
Gln Met Tyr Arg Ser Leu Glu Tyr Gln Val Asp Ala Ile 740 745 750Lys
Lys Ile Ile Asp Tyr Glu Tyr Lys Ile Tyr Ser Gly Pro Asp Lys 755 760
765Glu Gln Ile Ala Asp Glu Ile Asn Asn Leu Lys Asn Lys Leu Glu Glu
770 775 780Lys Ala Asn Lys Ala Met Ile Asn Ile Asn Ile Phe Met Arg
Glu Ser785 790 795 800Ser Arg Ser Phe Leu Val Asn Gln Met Ile Asn
Glu Ala Lys Lys Gln 805 810 815Leu Leu Glu Phe Asp Thr Gln Ser Lys
Asn Ile Leu Met Gln Tyr Ile 820 825 830Lys Ala Asn Ser Lys Phe Ile
Gly Ile Thr Glu Leu Lys Lys Leu Glu 835 840 845Ser Lys Ile Asn Lys
Val Phe Ser Thr Pro Ile Pro Phe Ser Tyr Ser 850
855 860Lys Asn Leu Asp Cys Trp Val Asp Asn Glu Glu Asp Ile Asp Val
Ile865 870 875 880Leu Lys Lys Ser Thr Ile Leu Asn Leu Asp Ile Asn
Asn Asp Ile Ile 885 890 895Ser Asp Ile Ser Gly Phe Asn Ser Ser Val
Ile Thr Tyr Pro Asp Ala 900 905 910Gln Leu Val Pro Gly Ile Asn Gly
Lys Ala Ile His Leu Val Asn Asn 915 920 925Glu Ser Ser Glu Val Ile
Val His Lys Ala Met Asp Ile Glu Tyr Asn 930 935 940Asp Met Phe Asn
Asn Phe Thr Val Ser Phe Trp Leu Arg Val Pro Lys945 950 955 960Val
Ser Ala Ser His Leu Glu Gln Tyr Gly Thr Asn Glu Tyr Ser Ile 965 970
975Ile Ser Ser Met Lys Lys His Ser Leu Ser Ile Gly Ser Gly Trp Ser
980 985 990Val Ser Leu Lys Gly Asn Asn Leu Ile Trp Thr Leu Lys Asp
Ser Ala 995 1000 1005Gly Glu Val Arg Gln Ile Thr Phe Arg Asp Leu
Pro Asp Lys Phe Asn 1010 1015 1020Ala Tyr Leu Ala Asn Lys Trp Val
Phe Ile Thr Ile Thr Asn Asp Arg1025 1030 1035 1040Leu Ser Ser Ala
Asn Leu Tyr Ile Asn Gly Val Leu Met Gly Ser Ala 1045 1050 1055Glu
Ile Thr Gly Leu Gly Ala Ile Arg Glu Asp Asn Asn Ile Thr Leu 1060
1065 1070Lys Leu Asp Arg Cys Asn Asn Asn Asn Gln Tyr Val Ser Ile
Asp Lys 1075 1080 1085Phe Arg Ile Phe Cys Lys Ala Leu Asn Pro Lys
Glu Ile Glu Lys Leu 1090 1095 1100Tyr Thr Ser Tyr Leu Ser Ile Thr
Phe Leu Arg Asp Phe Trp Gly Asn1105 1110 1115 1120Pro Leu Arg Tyr
Asp Thr Glu Tyr Tyr Leu Ile Pro Val Ala Ser Ser 1125 1130 1135Ser
Lys Asp Val Gln Leu Lys Asn Ile Thr Asp Tyr Met Tyr Leu Thr 1140
1145 1150Asn Ala Pro Ser Tyr Thr Asn Gly Lys Leu Asn Ile Tyr Tyr
Arg Arg 1155 1160 1165Leu Tyr Asn Gly Leu Lys Phe Ile Ile Lys Arg
Tyr Thr Pro Asn Asn 1170 1175 1180Glu Ile Asp Ser Phe Val Lys Ser
Gly Asp Phe Ile Lys Leu Tyr Val1185 1190 1195 1200Ser Tyr Asn Asn
Asn Glu His Ile Val Gly Tyr Pro Lys Asp Gly Asn 1205 1210 1215Ala
Phe Asn Asn Leu Asp Arg Ile Leu Arg Val Gly Tyr Asn Ala Pro 1220
1225 1230Gly Ile Pro Leu Tyr Lys Lys Met Glu Ala Val Lys Leu Arg
Asp Leu 1235 1240 1245Lys Thr Tyr Ser Val Gln Leu Lys Leu Tyr Asp
Asp Lys Asn Ala Ser 1250 1255 1260Leu Gly Leu Val Gly Thr His Asn
Gly Gln Ile Gly Asn Asp Pro Asn1265 1270 1275 1280Arg Asp Ile Leu
Ile Ala Ser Asn Trp Tyr Phe Asn His Leu Lys Asp 1285 1290 1295Lys
Ile Leu Gly Cys Asp Trp Tyr Phe Val Pro Thr Asp Glu Gly Trp 1300
1305 1310Thr Asn Asp 13159293PRTClostridium botulinum serotype A
9Met Glu His Tyr Ser Val Ile Gln Asn Ser Leu Asn Asp Lys Ile Val1 5
10 15Thr Ile Ser Cys Lys Ala Asp Thr Asn Leu Phe Phe Tyr Gln Val
Ala 20 25 30Gly Asn Val Ser Leu Phe Gln Gln Thr Arg Asn Tyr Leu Glu
Arg Trp 35 40 45Arg Leu Ile Tyr Asp Ser Asn Lys Ala Ala Tyr Lys Ile
Lys Ser Met 50 55 60Asp Ile His Asn Thr Asn Leu Val Leu Thr Trp Asn
Ala Pro Thr His65 70 75 80Asn Ile Ser Thr Gln Gln Asp Ser Asn Ala
Asp Asn Gln Tyr Trp Leu 85 90 95Leu Leu Lys Asp Ile Gly Asn Asn Ser
Phe Ile Ile Ala Ser Tyr Lys 100 105 110Asn Pro Asn Leu Val Leu Tyr
Ala Asp Thr Val Ala Arg Asn Leu Lys 115 120 125Leu Ser Thr Leu Asn
Asn Ser Asn Tyr Ile Lys Phe Ile Ile Glu Asp 130 135 140Tyr Ile Ile
Ser Asp Leu Asn Asn Phe Thr Cys Lys Ile Ser Pro Ile145 150 155
160Leu Asp Leu Asn Lys Val Val Gln Gln Val Asp Val Thr Asn Leu Asn
165 170 175Val Asn Leu Tyr Thr Trp Asp Tyr Gly Arg Asn Gln Lys Trp
Thr Ile 180 185 190Arg Tyr Asn Glu Glu Lys Ala Ala Tyr Gln Phe Phe
Asn Thr Ile Leu 195 200 205Ser Asn Gly Val Leu Thr Trp Ile Phe Ser
Asn Gly Asn Thr Val Arg 210 215 220Val Ser Ser Ser Asn Asp Gln Asn
Asn Asp Ala Gln Tyr Trp Leu Ile225 230 235 240Asn Pro Val Ser Asp
Thr Asp Glu Thr Tyr Thr Ile Thr Asn Leu Arg 245 250 255Asp Thr Thr
Lys Ala Leu Asp Leu Tyr Gly Gly Gln Thr Ala Asn Gly 260 265 270Thr
Ala Ile Gln Val Phe Asn Tyr His Gly Asp Asp Asn Gln Lys Trp 275 280
285Asn Ile Arg Asn Pro 29010293PRTClostridium botulinum serotype A
10Met Glu His Tyr Ser Val Ile Gln Asn Ser Leu Asn Asp Lys Ile Val1
5 10 15Thr Ile Ser Cys Lys Ala Asp Thr Asn Leu Phe Phe Tyr Gln Val
Ala 20 25 30Gly Asn Val Ser Leu Phe Gln Gln Thr Arg Asn Tyr Leu Glu
Arg Trp 35 40 45Arg Leu Ile Tyr Asp Ser Asn Lys Ala Ala Tyr Lys Ile
Lys Ser Met 50 55 60Asp Ile His Asn Thr Asn Leu Val Leu Thr Trp Asn
Ala Pro Thr His65 70 75 80Asn Ile Ser Thr Gln Gln Asp Ser Asn Ala
Asp Asn Gln Tyr Trp Leu 85 90 95Leu Leu Lys Asp Ile Gly Asn Asn Ser
Phe Ile Ile Ala Ser Tyr Lys 100 105 110Asn Pro Asn Leu Val Leu Tyr
Ala Asp Thr Val Ala Arg Asn Leu Lys 115 120 125Leu Ser Thr Leu Asn
Asn Ser Asn Tyr Ile Lys Phe Ile Ile Glu Asp 130 135 140Tyr Ile Ile
Ser Asp Leu Asn Asn Phe Thr Cys Lys Ile Ser Pro Ile145 150 155
160Leu Asp Arg Asn Lys Val Val Gln Gln Val Asp Met Thr Asn Leu Asn
165 170 175Val Asn Leu Tyr Thr Trp Asp Tyr Gly Arg Asn Gln Lys Trp
Thr Ile 180 185 190Arg Tyr Asn Glu Glu Lys Ala Ala Tyr Gln Phe Phe
Asn Thr Ile Leu 195 200 205Ser Asn Gly Val Leu Thr Trp Ile Phe Ser
Asn Gly Asn Thr Val Arg 210 215 220Val Ser Ser Ser Asn Asp Gln Asn
Asn Asp Ala Gln Tyr Trp Leu Ile225 230 235 240Asn Pro Val Ser Asp
Thr Asp Glu Thr Tyr Thr Ile Thr Asn Leu Arg 245 250 255Asp Thr Thr
Lys Ala Leu Asp Leu Tyr Asn Ser Gln Thr Ala Asn Gly 260 265 270Thr
Ala Ile Gln Val Phe Asn Tyr His Gly Asp Asp Asn Gln Lys Trp 275 280
285Asn Ile Arg Asn Pro 29011293PRTClostridium botulinum serotype A
11Met Glu His Tyr Ser Val Ile Gln Asn Ser Leu Asn Asp Lys Ile Val1
5 10 15Thr Ile Ser Cys Arg Ala Asp Thr Asn Leu Phe Phe Tyr Gln Val
Ala 20 25 30Gly Asn Val Ser Leu Phe Gln Gln Thr Arg Asn Tyr Leu Glu
Arg Trp 35 40 45Arg Ile Ile Tyr Asp Ser Asn Lys Ala Ala Tyr Lys Ile
Lys Ser Met 50 55 60Asp Ile His Asn Thr Asn Leu Val Leu Thr Trp Asn
Ala Pro Thr His65 70 75 80Asn Ile Ser Thr Gln Gln Asp Ser Asn Ala
Asp Asn Gln Tyr Trp Leu 85 90 95Leu Leu Lys Asp Ile Gly Asn Asn Ser
Phe Ile Ile Ala Ser Tyr Lys 100 105 110Asn Pro Asn Leu Val Leu Tyr
Ala Asp Thr Val Ala Arg Asn Leu Lys 115 120 125Leu Ser Thr Leu Asn
Asn Ser Asn Tyr Ile Lys Phe Ile Ile Glu Asp 130 135 140Tyr Ile Ile
Ser Asp Phe Asn Asn Phe Thr Cys Lys Ile Ser Pro Ile145 150 155
160Leu Asp Arg Asn Lys Val Val Gln Gln Val Ala Thr Thr Asn Leu Asn
165 170 175Val Asn Leu Tyr Thr Trp Asp Tyr Gly Arg Asn Gln Lys Trp
Thr Ile 180 185 190Arg Tyr Asn Glu Glu Lys Ala Ala Tyr Gln Phe Phe
Asn Thr Ile Leu 195 200 205Ser Asn Gly Val Leu Thr Trp Ile Phe Ser
Asn Gly Asn Thr Val Arg 210 215 220Val Ser Ser Ser Asn Asp Gln Asn
Asn Asp Ala Gln Tyr Trp Leu Ile225 230 235 240Asn Pro Val Ser Asp
Thr Asp Glu Thr Tyr Thr Ile Thr Asn Leu Arg 245 250 255Asp Thr Thr
Lys Ala Leu Asp Leu Tyr Asn Ser Gln Thr Ala Asn Gly 260 265 270Thr
Ala Ile Gln Val Phe Asn Ser Asn Gly Gly Asp Asn Gln Lys Trp 275 280
285Asn Ile Arg Asn Pro 29012294PRTClostridium botulinum serotype A
12Met Glu His Tyr Ser Thr Ile Gln Asn Ser Leu Asn Asp Lys Ile Val1
5 10 15Thr Ile Ser Cys Lys Ala Asn Thr Asp Leu Phe Phe Tyr Gln Val
Pro 20 25 30Gly Asn Gly Asn Val Ser Leu Phe Gln Gln Thr Arg Asn Tyr
Leu Glu 35 40 45Arg Trp Arg Ile Ile Tyr Asp Ser Asn Lys Ala Ala Tyr
Lys Ile Lys 50 55 60Ser Met Asn Ile Tyr Asn Thr Asn Leu Val Leu Thr
Trp Asn Ala Pro65 70 75 80Thr His Asn Ile Ser Ala Gln Gln Asp Ser
Asn Ala Asp Asn Gln Tyr 85 90 95Trp Leu Leu Leu Lys Asp Ile Gly Asn
Asn Ser Phe Ile Ile Ala Ser 100 105 110Tyr Lys Asn Pro Asn Leu Val
Leu Tyr Ala Asp Thr Val Ala Arg Asn 115 120 125Leu Lys Leu Ser Thr
Leu Asn Asn Ser Ser Tyr Ile Lys Phe Ile Ile 130 135 140Glu Asp Tyr
Val Ile Ser Asp Phe Lys Asn Phe Thr Cys Arg Ile Ser145 150 155
160Pro Ile Leu Ala Gly Gly Lys Val Val Gln Gln Val Ser Met Thr Asn
165 170 175Leu Ala Val Asn Leu Tyr Ile Trp Asn Asn Asp Leu Asn Gln
Lys Trp 180 185 190Thr Ile Ile Tyr Asn Glu Glu Lys Ala Ala Tyr Gln
Phe Phe Asn Lys 195 200 205Ile Leu Ser Asn Gly Val Leu Thr Trp Ile
Phe Ser Asp Gly Asn Thr 210 215 220Val Arg Val Ser Ser Ser Ala Gln
Asn Asn Asp Ala Gln Tyr Trp Leu225 230 235 240Ile Asn Pro Val Ser
Asp Asn Tyr Asp Arg Tyr Thr Ile Thr Asn Leu 245 250 255Arg Asp Lys
Thr Lys Val Leu Asp Leu Tyr Gly Gly Gln Thr Ala Asp 260 265 270Gly
Thr Thr Ile Gln Val Phe Asn Ser Asn Gly Gly Asp Asn Gln Ile 275 280
285Trp Thr Met Ser Asn Pro 29013279PRTClostridium botulinum
serotype A 13Met Glu His Tyr Ser Val Ile Gln Asn Ser Leu Asn Asp
Lys Ile Val1 5 10 15Thr Ile Ser Cys Lys Ala Asp Thr Asn Leu Phe Phe
Tyr Gln Val Ala 20 25 30Gly Asn Val Ser Leu Phe Gln Gln Thr Arg Asn
Tyr Leu Glu Arg Trp 35 40 45Arg Leu Ile Tyr Asp Ser Asn Lys Ala Ala
Tyr Lys Ile Lys Ser Met 50 55 60Asp Ile His Asn Thr Asn Leu Val Leu
Thr Trp Asn Ala Pro Thr His65 70 75 80Asn Ile Ser Thr Gln Gln Asp
Ser Asn Ala Asp Asn Gln Tyr Trp Leu 85 90 95Leu Leu Lys Asp Ile Gly
Asn Asn Ser Phe Ile Ile Ala Ser Tyr Lys 100 105 110Asn Pro Asn Leu
Val Leu Tyr Ala Asp Thr Val Ala Arg Asn Leu Lys 115 120 125Leu Ser
Thr Leu Asn Asn Ser Asn Tyr Ile Lys Phe Ile Ile Glu Asp 130 135
140Tyr Ile Ile Ser Asp Leu Asn Asn Phe Thr Cys Lys Ile Ser Pro
Ile145 150 155 160Leu Asp Arg Asn Lys Val Val Gln Gln Val Asp Met
Thr Asn Leu Asn 165 170 175Val Asn Leu Tyr Thr Trp Asp Tyr Gly Arg
Asn Gln Lys Trp Thr Ile 180 185 190Arg Tyr Asn Glu Glu Lys Ala Ala
Tyr Gln Phe Phe Asn Thr Ile Leu 195 200 205Ser Asn Gly Val Leu Thr
Trp Ile Phe Ser Asn Gly Asn Thr Val Arg 210 215 220Val Ser Ser Ser
Asn Asp Gln Asn Asn Asp Ala Gln Tyr Trp Leu Ile225 230 235 240Asn
Pro Val Ser Asp Thr Asp Glu Thr Tyr Thr Ile Thr Asn Leu Arg 245 250
255Asp Thr Thr Lys Ala Leu Asp Leu Tyr Asn Ser Gln Thr Ala Asn Gly
260 265 270Thr Ala Ile Gln Val Phe Asn 27514292PRTClostridium
botulinum serotype B 14Met Glu His Tyr Ser Val Ile Gln Asn Ser Leu
Asn Asp Glu Ile Val1 5 10 15Thr Ile Ser Cys Lys Ala Asp Thr Asn Leu
Phe Phe Tyr Gln Thr Val 20 25 30Gly Asn Val Ser Leu Phe Gln Gln Thr
Arg Asn Tyr Leu Glu Arg Trp 35 40 45Arg Leu Ile Tyr Asp Ala Asn Lys
Ala Ala Tyr Lys Ile Lys Ser Met 50 55 60Asp Ser His Asn Thr Asn Leu
Val Leu Thr Trp Asn Ala Pro Thr His65 70 75 80Asn Ile Ser Ala Gln
Gln Asp Ser Asn Ala Asp Asn Gln Tyr Trp Leu 85 90 95Leu Leu Lys Asp
Ile Gly Ser Asn Ser Phe Ile Ile Ala Ser Tyr Lys 100 105 110Asn Pro
Asn Leu Val Leu Tyr Ala Asp Thr Val Ala Arg Asn Leu Lys 115 120
125Leu Ser Thr Leu Asn Asn Ser Ser Tyr Ile Lys Phe Ile Ile Glu Asp
130 135 140Tyr Met Ile Ser Asp Phe Asn Asn Phe Thr Cys Lys Ile Ser
Pro Ile145 150 155 160Leu Asp Ser Ser Lys Val Val Gln Gln Val Ala
Met Thr Asp Leu Ser 165 170 175Val Asn Leu Tyr Thr Trp Asp Tyr Gly
Arg Asn Gln Lys Trp Thr Ile 180 185 190Lys Tyr Asn Lys Glu Lys Ser
Ala Tyr Gln Phe Phe Asn Thr Ile Leu 195 200 205Ser Asn Gly Val Leu
Thr Trp Ile Ser Ser Asn Gly Asn Thr Val Arg 210 215 220Val Ser Ser
Ile Ala Gln Asn Asn Asp Ala Gln Tyr Trp Leu Ile Asn225 230 235
240Pro Val Ser Asn Ala Tyr Glu Thr Tyr Thr Ile Thr Asn Leu His Asp
245 250 255Thr Thr Lys Ala Leu Asp Leu Tyr Asn Ser Gln Thr Ala Asn
Gly Thr 260 265 270Thr Ile Gln Val Phe Asn Tyr His Gly Asp Asp Asn
Gln Lys Trp Phe 275 280 285Ile Arg Asn Pro 29015291PRTClostridium
botulinum serotype B 15Met Glu His Tyr Ser Thr Ile Gln Asn Ser Leu
Asn Asp Lys Ile Val1 5 10 15Thr Ile Ser Cys Lys Ala Asn Thr Asp Leu
Phe Phe Tyr Gln Val Pro 20 25 30Gly Asn Gly Asn Val Ser Leu Phe Gln
Gln Thr Arg Asn Tyr Leu Glu 35 40 45Arg Trp Arg Ile Ile Tyr Asp Ser
Asn Lys Ala Ala Tyr Lys Ile Lys 50 55 60Ser Met Asn Ile Tyr Asn Thr
Asn Leu Val Leu Thr Trp Asn Ala Pro65 70 75 80Thr His Asn Ile Ser
Ala Leu Gln Asp Ser Asn Ala Asp Asn Gln Tyr 85 90 95Trp Leu Leu Leu
Lys Asp Ile Gly Asn Asn Ser Phe Ile Ile Ala Ser 100 105 110Tyr Lys
Asn Pro Asn Leu Val Leu Tyr Ala Asp Thr Val Ala Arg Asn 115 120
125Leu Lys Leu Ser Thr Leu Asn Asn Ser Ser Tyr Ile Lys Phe Ile Ile
130 135 140Glu Asp Tyr Val Ile Ser Asp Phe Lys Asn Phe Thr Cys Arg
Ile Ser145 150 155 160Pro Ile Leu Ala Gly Gly Lys Val Val Gln Gln
Val Ser Met Thr Asn 165 170 175Leu Ala Val Asn Leu Tyr Ile Trp Asn
Asn Asp Leu Asn Gln Lys Trp 180 185 190Thr Ile Ile Tyr Asn Glu Glu
Lys Ala Ala Tyr Gln Phe Phe Asn Lys 195 200 205Ile Leu Ser Asn Gly
Val Leu Thr Trp Ile Phe Ser Asp Gly Asn Thr 210 215 220Val Arg Val
Ser Ser Ser Ala Gln Asn Asp Ala Gln Tyr Trp Leu Ile225 230 235
240Asn Pro Val Ser Asp Asn Tyr Asp Arg Tyr Thr Ile Thr Asn Leu Arg
245 250 255Tyr Lys Thr Lys Val Leu Asp Leu Tyr Gly Gly Gln Thr Ala
Asp Gly 260 265 270Thr Thr Ile Gln Val Phe Asn Ser Asn Gly Gly Asp
Asn Gln Ile Trp 275 280 285Tyr Gly Leu 29016286PRTClostridium
botulinum serotype C1 16Met Ser Gln Thr Asn Ala Asn Asp Leu Arg Asn
Asn Glu Val Phe Phe1 5 10 15Ile Ser Pro Ser Asn Asn Thr Asn Lys Val
Leu Asp Lys Ile Ser Gln 20 25 30Ser Glu Val Lys Leu Trp Asn Lys Leu
Ser Gly Ala Asn Gln Lys Trp 35 40 45Arg Leu Ile Tyr Asp Thr Asn Lys
Gln Ala Tyr Lys Ile Lys Val Met 50 55 60Asp Asn Thr Ser Leu Ile Leu
Thr Trp Asn Ala Pro Leu Ser Ser Val65 70 75 80Ser Val Lys Thr Asp
Thr Asn Gly Asp Asn Gln Tyr Trp Tyr Leu Leu 85 90 95Gln Asn Tyr Ile
Ser Arg Asn Val Ile Ile Arg Asn Tyr Met Asn Pro 100 105 110Asn Leu
Val Leu Gln Tyr Asn Ile Asp Asp Thr Leu Met Val Ser Thr 115 120
125Gln Thr Ser Ser Ser Asn Gln Phe Phe Lys Phe Ser Asn Cys Ile Tyr
130 135 140Glu Ala Leu Asn Asn Arg Asn Cys Lys Leu Gln Thr Gln Leu
Asn Ser145 150 155 160Asp Arg Phe Leu Ser Lys Asn Leu Asn Ser Gln
Ile Ile Val Leu Trp 165 170 175Gln Trp Phe Asp Ser Ser Arg Gln Lys
Trp Ile Ile Glu Tyr Asn Glu 180 185 190Thr Lys Ser Ala Tyr Thr Leu
Lys Cys Gln Glu Asn Asn Arg Tyr Leu 195 200 205Thr Trp Ile Gln Asn
Ser Asn Asn Tyr Val Glu Thr Tyr Gln Ser Thr 210 215 220Asp Ser Leu
Ile Gln Tyr Trp Asn Ile Asn Tyr Leu Asp Asn Asp Ala225 230 235
240Ser Lys Tyr Ile Leu Tyr Asn Leu Gln Asp Thr Asn Arg Val Leu Asp
245 250 255Val Tyr Asn Ser Gln Ile Ala Asn Gly Thr His Val Ile Val
Asp Ser 260 265 270Tyr His Gly Asn Thr Asn Gln Gln Trp Ile Ile Asn
Leu Ile 275 280 28517285PRTClostridium botulinum serotype C1 17Met
Ser Gln Thr Asn Ala Asn Asp Leu Arg Asn Asn Glu Val Phe Phe1 5 10
15Ile Ser Pro Ser Asn Asn Thr Asn Lys Val Leu Asp Lys Ile Ser Gln
20 25 30Ser Glu Val Lys Leu Trp Asn Lys Leu Ser Gly Ala Asn Gln Lys
Trp 35 40 45Arg Leu Ile Tyr Asp Thr Asn Lys Gln Ala Tyr Lys Ile Lys
Val Met 50 55 60Asp Asn Thr Ser Leu Ile Leu Thr Trp Asn Ala Pro Leu
Ser Ser Val65 70 75 80Ser Val Lys Thr Asp Thr Asn Gly Asp Asn Gln
Tyr Trp Tyr Leu Leu 85 90 95Gln Asn Tyr Ile Ser Arg Asn Val Ile Ile
Arg Asn Tyr Met Asn Pro 100 105 110Asn Leu Val Leu Gln Tyr Asn Ile
Asp Asp Thr Leu Met Val Ser Thr 115 120 125Gln Thr Ser Ser Ser Asn
Gln Phe Phe Lys Phe Ser Asn Cys Ile Tyr 130 135 140Glu Ser Phe Asn
Asn Ser Thr Cys Lys Ile Gln Thr Ser Leu Thr Ile145 150 155 160Lys
Phe Ile Asp Lys Asn Gln Asn Ser Asn Asn Val Thr Ile Trp Ser 165 170
175Trp Asn Asn Gly Asp Asn Gln Lys Trp Lys Ile Leu Tyr Asn Glu Ser
180 185 190Lys Met Ala Tyr Thr Leu Thr Cys Ile Lys Asn Asn Glu Tyr
Leu Thr 195 200 205Trp Phe Ser Ser Ile Gly Asn Asn Val Gly Thr Tyr
Arg Thr Glu Gly 210 215 220Asn Asn Asp Gln Tyr Trp Phe Ile Asn Tyr
Leu Asn Asn Asp Ala Ser225 230 235 240Met Tyr Thr Ile Ser Asn Phe
Ser Asn Gln Ser Lys Phe Leu Asp Val 245 250 255Val Asn Ser Gly Leu
Ala Asp Gly Thr Asn Val Gln Val Trp Asp Ser 260 265 270Asn Gly Thr
Ser Ala Gln Lys Trp Ile Ile Thr Arg Leu 275 280
28518286PRTClostridium botulinum serotype D 18Met Ser Gln Thr Asn
Ala Asn Asp Leu Arg Asn Asn Glu Val Phe Phe1 5 10 15Ile Ser Pro Ser
Asn Asn Thr Asn Lys Val Leu Asp Lys Ile Ser Gln 20 25 30Ser Glu Val
Lys Leu Trp Asn Lys Leu Ser Gly Ala Asn Gln Lys Trp 35 40 45Arg Leu
Ile Tyr Asp Thr Asn Lys Gln Ala Tyr Thr Ile Lys Val Met 50 55 60Asp
Asn Thr Ser Leu Ile Leu Thr Trp Asp Ala Pro Leu Ser Ser Val65 70 75
80Ser Val Lys Thr Asp Thr Asn Thr Asn Asn Gln Tyr Trp Tyr Leu Leu
85 90 95Gln Asp Tyr Ile Ser Arg Asn Val Ile Leu Arg Asn Tyr Met Asn
Pro 100 105 110Asn Leu Val Leu Gln Tyr Asn Thr Asp Asp Thr Leu Ile
Val Ser Thr 115 120 125Gln Thr Asn Ser Asn Asn Gln Phe Phe Lys Phe
Ser Asn Cys Ile Tyr 130 135 140Glu Ala Leu Asn Asn Arg Asn Cys Lys
Leu Gln Thr Gln Leu Asn Ser145 150 155 160Asp Arg Phe Leu Ser Lys
Asn Leu Asn Ser Gln Ile Ile Val Leu Trp 165 170 175Gln Trp Phe Asp
Ser Ser Arg Gln Lys Trp Thr Ile Glu Tyr Asn Glu 180 185 190Thr Lys
Ser Ala Tyr Thr Leu Lys Cys Gln Glu Asn Asn Arg Tyr Leu 195 200
205Thr Trp Ile Gln Asn Ser Asn Asn Tyr Val Glu Thr Tyr Gln Ser Thr
210 215 220Asp Ser Leu Ile Gln Tyr Trp Asn Ile Asn Tyr Leu Asp Asn
Asp Ala225 230 235 240Ser Lys Tyr Ile Leu Tyr Asn Leu Gln Asp Thr
Asn Arg Val Leu Asp 245 250 255Val Tyr Asn Ser Gln Thr Ala Asn Gly
Thr His Val Ile Val Asp Ser 260 265 270Tyr His Gly Asn Thr Asn Gln
Gln Trp Ile Ile Asn Leu Ile 275 280 28519146PRTClostridium
botulinum serotype A 19Met Ser Val Glu Arg Thr Phe Leu Pro Asn Gly
Asn Tyr Asn Ile Lys1 5 10 15Ser Ile Phe Ser Gly Ser Leu Tyr Leu Asn
Pro Val Ser Lys Ser Leu 20 25 30Thr Phe Ser Asn Glu Ser Ser Ala Asn
Asn Gln Lys Trp Asn Val Glu 35 40 45Tyr Met Ala Glu Asn Arg Cys Phe
Lys Ile Ser Asn Val Ala Glu Pro 50 55 60Asn Lys Tyr Leu Ser Tyr Asp
Asn Phe Gly Phe Ile Ser Leu Asp Ser65 70 75 80Leu Ser Asn Arg Cys
Tyr Trp Phe Pro Ile Lys Ile Ala Val Asn Thr 85 90 95Tyr Ile Met Leu
Ser Leu Asn Lys Val Asn Glu Leu Asp Tyr Ala Trp 100 105 110Asp Ile
Tyr Asp Thr Asn Glu Asn Ile Leu Ser Gln Pro Leu Leu Leu 115 120
125Leu Pro Asn Phe Asp Ile Tyr Asn Ser Asn Gln Met Phe Lys Leu Glu
130 135 140Lys Ile14520146PRTClostridium botulinum serotype B 20Met
Ser Ala Glu Arg Thr Phe Leu Pro Asn Gly Asn Tyr Asn Ile Lys1 5 10
15Ser Ile Phe Ser Gly Ser Leu Tyr Leu Ser Pro Val Ser Gly Ser Leu
20 25 30Thr Phe Ser Asn Glu Ser Ser Ala Asn Asn Gln Lys Trp Asn Val
Glu 35 40 45Tyr Met Ala Glu Asn Arg Cys Phe Lys Ile Ser Asn Val Ala
Glu Pro 50 55 60Asn Lys Tyr Leu Ser Tyr Asp Asn Phe Gly Phe Ile Ser
Leu Asp Ser65 70 75 80Leu Ser Asn Arg Cys Tyr Trp Phe Pro Ile Lys
Ile Ala Val Asn Thr 85 90 95Tyr Ile Met Leu Ser Leu Asn Lys Val Asn
Glu Leu Asp Tyr Ala Trp 100 105 110Asp Ile Tyr Asp Thr Asn Glu Asn
Ile Leu Ser Gln Pro Leu Leu Leu 115 120 125Leu Pro Asn Phe Asp Ile
Tyr Asn Ser Asn Gln Met Phe Lys Leu Glu 130 135 140Lys
Ile14521146PRTClostridium botulinum serotype C1 21Met Ser Ser Glu
Arg Thr Phe Leu Pro Asn Gly Asn Tyr Lys Ile Lys1 5 10 15Ser Leu Phe
Ser Asn Ser Leu Tyr Leu Thr Tyr Ser Ser Gly Ala Leu 20 25 30Ser Phe
Ser Asn Thr Ser Ser Leu Asp Asn Gln Lys Trp Lys Leu Glu 35 40 45Tyr
Ile Ser Ser Ser Asn Gly Phe Arg Phe Ser Asn Val Ala Glu Pro 50 55
60Asn Lys Tyr Leu Ala Tyr Asn Asp Tyr Gly Phe Ile Tyr Leu Ser Ser65
70 75 80Ser Ser Asn Asn Ser Leu Trp Asn Pro Ile Lys Ile Ala Ile Asn
Ser 85 90 95Tyr Ile Ile Cys Thr Leu Ser Ile Val Asn Val Thr Asp Tyr
Ala Trp 100 105 110Thr Ile Tyr Asp Asn Asn Asn Asn Ile Thr Asp Gln
Pro Ile Leu Asn 115 120 125Leu Pro Asn Phe Asp Ile Asn Asn Ser Asn
Gln Ile Leu Lys Leu Glu 130 135 140Lys Leu14522146PRTClostridium
botulinum serotype D 22Met Ser Ser Glu Arg Thr Phe Leu Pro Asn Gly
Asn Tyr Lys Ile Lys1 5 10 15Ser Leu Phe Ser Asp Ser Leu Tyr Leu Thr
Tyr Ser Ser Gly Ser Leu 20 25 30Ser Phe Leu Asn Thr Ser Ser Leu Asp
Asn Gln Lys Trp Lys Leu Glu 35 40 45Tyr Ile Ser Ser Ser Asn Gly Phe
Arg Phe Ser Asn Val Ala Glu Pro 50 55 60Asn Lys Tyr Leu Ala Tyr Asn
Asp Tyr Gly Phe Ile Tyr Leu Ser Ser65 70 75 80Ser Ser Asn Asn Ser
Leu Trp Asn Pro Ile Lys Ile Ala Ile Asn Ser 85 90 95Tyr Ile Ile Cys
Thr Leu Ser Ile Val Asn Val Thr Asp Tyr Ala Trp 100 105 110Thr Ile
Tyr Asp Asn Asn Asn Asn Ile Thr Asp Gln Pro Ile Leu Asn 115 120
125Leu Pro Asn Phe Asp Ile Asn Asn Ser Asn Gln Ile Leu Lys Leu Glu
130 135 140Lys Leu145231193PRTClostridium botulinum serotype A
23Met Asn Ile Asn Asp Asn Leu Ser Ile Asn Ser Pro Val Asp Asn Lys1
5 10 15Asn Val Val Val Val Arg Ala Arg Lys Thr Asp Thr Val Phe Lys
Ala 20 25 30Phe Lys Val Ala Pro Asn Ile Trp Val Ala Pro Glu Arg Tyr
Tyr Gly 35 40 45Glu Ser Leu Ser Ile Asp Glu Glu Tyr Lys Val Asp Gly
Gly Ile Tyr 50 55 60Asp Ser Asn Phe Leu Ser Gln Asp Ser Glu Lys Asp
Lys Phe Leu Gln65 70 75 80Ala Ile Ile Thr Leu Leu Lys Arg Ile Asn
Ser Thr Asn Ala Gly Glu 85 90 95Lys Leu Leu Ser Leu Ile Ser Thr Ala
Ile Pro Phe Pro Tyr Gly Tyr 100 105 110Ile Gly Gly Gly Tyr Tyr Ala
Pro Asn Met Ile Thr Phe Gly Ser Ala 115 120 125Pro Lys Ser Asn Lys
Lys Leu Asn Ser Leu Ile Ser Ser Thr Ile Pro 130 135 140Phe Pro Tyr
Ala Gly Tyr Arg Glu Thr Asn Tyr Leu Ser Ser Glu Asp145 150 155
160Asn Lys Ser Phe Tyr Ala Ser Asn Ile Val Ile Phe Gly Pro Gly Ala
165 170 175Asn Ile Val Glu Asn Asn Thr Val Phe Tyr Lys Lys Glu Asp
Ala Glu 180 185 190Asn Gly Met Gly Thr Met Thr Glu Ile Trp Phe Gln
Pro Phe Leu Thr 195 200 205Tyr Lys Tyr Asp Glu Phe Tyr Ile Asp Pro
Ala Ile Glu Leu Ile Lys 210 215 220Cys Leu Ile Lys Ser Leu Tyr Phe
Leu Tyr Gly Ile Lys Pro Ser Asp225 230 235 240Asp Leu Val Ile Pro
Tyr Arg Leu Arg Ser Glu Leu Glu Asn Ile Glu 245 250 255Tyr Ser Gln
Leu Asn Ile Val Asp Leu Leu Val Ser Gly Gly Ile Asp 260 265 270Pro
Lys Phe Ile Asn Thr Asp Pro Tyr Trp Phe Thr Asp Asn Tyr Phe 275 280
285Ser Asn Ala Lys Lys Val Phe Glu Asp His Arg Asn Ile Tyr Glu Thr
290 295 300Glu Ile Glu Gly Asn Asn Ala Ile Gly Asn Asp Ile Lys Leu
Arg Leu305 310 315 320Lys Gln Lys Phe Arg Ile Asn Ile Asn Asp Ile
Trp Glu Leu Asn Leu 325 330 335Asn Tyr Phe Ser Lys Glu Phe Ser Ile
Met Met Pro Asp Arg Phe Asn 340 345 350Asn Ala Leu Lys His Phe Tyr
Arg Lys Gln Tyr Tyr Lys Ile Asp Tyr 355 360 365Pro Glu Asn Tyr Ser
Ile Asn Gly Phe Val Asn Gly Gln Ile Asn Ala 370 375 380Gln Leu Ser
Leu Ser Asp Arg Asn Gln Asp Ile Ile Asn Lys Pro Glu385 390 395
400Glu Ile Ile Asn Leu Leu Asn Gly Asn Asn Val Ser Leu Met Arg Ser
405 410 415Asn Ile Tyr Gly Asp Gly Leu Lys Ser Thr Val Asp Asp Phe
Tyr Ser 420 425 430Asn Tyr Lys Ile Pro Tyr Asn Arg Ala Tyr Glu Tyr
His Phe Asn Asn 435 440 445Ser Asn Asp Ser Ser Leu Asp Asn Val Asn
Ile Gly Val Ile Asp Asn 450 455 460Ile Pro Glu Ile Ile Asp Val Asn
Pro Tyr Lys Glu Asn Cys Asp Lys465 470 475 480Phe Ser Pro Val Gln
Lys Ile Thr Ser Thr Arg Glu Ile Asn Thr Asn 485 490 495Ile Pro Trp
Pro Ile Asn Tyr Leu Gln Ala Gln Asn Thr Asn Asn Glu 500 505 510Lys
Phe Ser Leu Ser Ser Asp Phe Val Glu Val Val Ser Ser Lys Asp 515 520
525Lys Ser Leu Val Tyr Ser Phe Leu Ser Asn Val Met Phe Tyr Leu Asp
530 535 540Ser Ile Lys Asp Asn Ser Pro Ile Asp Thr Asp Lys Lys Tyr
Tyr Leu545 550 555 560Trp Leu Arg Glu Ile Phe Arg Asn Tyr Ser Phe
Asp Ile Thr Ala Thr 565 570 575Gln Glu Ile Asn Thr Asn Cys Gly Ile
Asn Lys Val Val Thr Trp Phe 580 585 590Gly Lys Ala Leu Asn Ile Leu
Asn Thr Ser Asp Ser Phe Val Glu Glu 595 600 605Phe Gln Asn Leu Gly
Ala Ile Ser Leu Ile Asn Lys Lys Glu Asn Leu 610 615 620Ser Met Pro
Ile Ile Glu Ser Tyr Glu Ile Pro Asn Asp Met Leu Gly625 630 635
640Leu Pro Leu Asn Asp Leu Asn Glu Lys Leu Phe Asn Ile Tyr Ser Lys
645 650 655Asn Thr Ala Tyr Phe Lys Lys Ile Tyr Tyr Asn Phe Leu Asp
Gln Trp 660 665 670Trp Thr Gln Tyr Tyr Ser Gln Tyr Phe Asp Leu Ile
Cys Met Ala Lys 675 680 685Arg Ser Val Leu Ala Gln Glu Thr Leu Ile
Lys Arg Ile Ile Gln Lys 690 695 700Lys Leu Ser Tyr Leu Ile Gly Asn
Ser Asn Ile Ser Ser Asp Asn Leu705 710 715 720Ala Leu Met Asn Leu
Thr Thr Thr Asn Thr Leu Arg Asp Ile Ser Asn 725 730 735Glu Ser Gln
Ile Ala Met Asn Asn Val Asp Ser Phe Leu Asn Asn Ala 740 745 750Ala
Ile Cys Val Phe Glu Ser Asn Ile Tyr Pro Lys Phe Ile Ser Phe 755 760
765Met Glu Gln Cys Ile Asn Asn Ile Asn Ile Lys Thr Lys Glu Phe Ile
770 775 780Gln Lys Cys Thr Asn Ile Asn Glu Asp Glu Lys Leu Gln Leu
Ile Asn785 790 795 800Gln Asn Val Phe Asn Ser Leu Asp Phe Glu Phe
Leu Asn Ile Gln Asn 805 810 815Met Lys Ser Leu Phe Ser Ser Glu Thr
Ala Leu Leu Ile Lys Glu Glu 820 825 830Thr Trp Pro Tyr Glu Leu Val
Leu Tyr Ala Phe Lys Glu Pro Gly Asn 835 840 845Asn Val Ile Gly Asp
Ala Ser Gly Lys Asn Thr Ser Ile Glu Tyr Ser 850 855 860Lys Asp Ile
Gly Leu Val Tyr Gly Ile Asn Ser Asp Ala Leu Tyr Leu865 870 875
880Asn Gly Ser Asn Gln Ser Ile Ser Phe Ser Asn Asp Phe Phe Glu Asn
885 890 895Gly Leu Thr Asn Ser Phe Ser Ile Tyr Phe Trp Leu Arg Asn
Leu Gly 900 905 910Lys Asp Thr Ile Lys Ser Lys Leu Ile Gly Ser Lys
Glu Asp Asn Cys 915 920 925Gly Trp Glu Ile Tyr Phe Gln Asp Thr Gly
Leu Val Phe Asn Met Ile 930
935 940Asp Ser Asn Gly Asn Glu Lys Asn Ile Tyr Leu Ser Asp Val Ser
Asn945 950 955 960Asn Ser Trp His Tyr Ile Thr Ile Ser Val Asp Arg
Leu Lys Glu Gln 965 970 975Leu Leu Ile Phe Ile Asp Asp Asn Leu Val
Ala Asn Glu Ser Ile Lys 980 985 990Glu Ile Leu Asn Ile Tyr Ser Ser
Asn Ile Ile Ser Leu Leu Ser Glu 995 1000 1005Asn Asn Pro Ser Tyr
Ile Glu Gly Leu Thr Ile Leu Asn Lys Pro Thr 1010 1015 1020Thr Ser
Gln Glu Val Leu Ser Asn Tyr Phe Glu Val Leu Asn Asn Ser1025 1030
1035 1040Tyr Ile Arg Asp Ser Asn Glu Glu Arg Leu Glu Tyr Asn Lys
Thr Tyr 1045 1050 1055Gln Leu Tyr Asn Tyr Val Phe Ser Asp Lys Pro
Ile Cys Glu Val Lys 1060 1065 1070Gln Asn Asn Asn Ile Tyr Leu Thr
Ile Asn Asn Thr Asn Asn Leu Asn 1075 1080 1085Leu Gln Ala Ser Lys
Phe Lys Leu Leu Ser Ile Asn Pro Asn Lys Gln 1090 1095 1100Tyr Val
Gln Lys Leu Asp Glu Val Ile Ile Ser Val Leu Asp Asn Met1105 1110
1115 1120Glu Lys Tyr Ile Asp Ile Ser Glu Asp Asn Arg Leu Gln Leu
Ile Asp 1125 1130 1135Asn Lys Asn Asn Ala Lys Lys Met Ile Ile Ser
Asn Asp Ile Phe Ile 1140 1145 1150Ser Asn Cys Leu Thr Leu Ser Tyr
Asn Gly Lys Tyr Ile Cys Leu Ser 1155 1160 1165Met Lys Asp Glu Asn
His Asn Trp Met Ile Cys Asn Asn Asp Met Ser 1170 1175 1180Lys Tyr
Leu Tyr Leu Trp Ser Phe Lys1185 1190241198PRTClostridium botulinum
serotype A 24Met Asn Ile Asn Asp Asn Leu Ser Ile Asn Ser Pro Val
Asp Asn Lys1 5 10 15Asn Val Val Val Val Arg Ala Arg Lys Thr Asp Thr
Val Phe Lys Ala 20 25 30Phe Lys Val Ala Pro Asn Ile Trp Val Ala Pro
Glu Arg Tyr Tyr Gly 35 40 45Glu Ser Leu Ser Ile Asp Glu Glu Tyr Lys
Val Asp Gly Gly Ile Tyr 50 55 60Asp Ser Asn Phe Leu Ser Gln Asp Ser
Glu Lys Asp Lys Phe Leu Gln65 70 75 80Ala Ile Ile Thr Leu Leu Lys
Arg Ile Asn Ser Thr Asn Ala Gly Glu 85 90 95Lys Leu Leu Ser Leu Ile
Ser Thr Ala Ile Pro Phe Pro Tyr Gly Tyr 100 105 110Ile Gly Gly Gly
Tyr Tyr Ala Pro Asn Met Ile Thr Phe Gly Ser Ala 115 120 125Pro Lys
Ser Asn Lys Lys Leu Asn Ser Leu Ile Ser Ser Thr Ile Pro 130 135
140Phe Pro Tyr Ala Gly Tyr Arg Glu Thr Asn Tyr Leu Ser Ser Glu
Asp145 150 155 160Asn Lys Ser Phe Tyr Ala Ser Asn Ile Val Ile Phe
Gly Pro Gly Ala 165 170 175Asn Ile Val Glu Asn Asn Thr Val Phe Tyr
Lys Lys Glu Asp Ala Glu 180 185 190Asn Gly Met Gly Thr Met Thr Glu
Ile Trp Phe Gln Pro Phe Leu Thr 195 200 205Tyr Lys Tyr Asp Glu Phe
Tyr Ile Asp Pro Ala Ile Glu Leu Ile Lys 210 215 220Cys Leu Ile Lys
Ser Leu Tyr Phe Leu Tyr Gly Ile Lys Pro Ser Asp225 230 235 240Asp
Leu Val Ile Pro Tyr Arg Leu Arg Ser Glu Leu Glu Asn Ile Glu 245 250
255Tyr Ser Gln Leu Asn Ile Val Asp Leu Leu Val Ser Gly Gly Ile Asp
260 265 270Pro Lys Phe Ile Asn Thr Asp Pro Tyr Trp Phe Ile Asp Asn
Tyr Phe 275 280 285Ser Asn Ala Lys Lys Val Phe Glu Asp His Arg Asn
Ile Tyr Glu Thr 290 295 300Glu Ile Glu Gly Asn Asn Ala Ile Gly Asn
Asp Ile Lys Leu Arg Leu305 310 315 320Lys Gln Lys Phe Arg Ile Asn
Ile Asn Asp Ile Trp Glu Leu Asn Leu 325 330 335Asn Tyr Phe Ser Lys
Glu Phe Ser Ile Met Met Pro Asp Arg Phe Asn 340 345 350Asn Ala Leu
Lys His Phe Tyr Arg Lys Gln Tyr Tyr Lys Ile Asp Tyr 355 360 365Pro
Glu Asn Tyr Ser Ile Asn Gly Phe Val Asn Gly Gln Ile Asn Ala 370 375
380Gln Leu Ser Leu Ser Asp Arg Asn Gln Asp Ile Ile Asn Lys Pro
Glu385 390 395 400Glu Ile Ile Asn Leu Leu Asn Gly Asn Asn Val Ser
Leu Met Arg Ser 405 410 415Asn Ile Tyr Gly Asp Gly Leu Lys Ser Thr
Val Asp Asp Phe Tyr Ser 420 425 430Asn Tyr Lys Ile Pro Tyr Asn Arg
Ala Tyr Glu Tyr His Phe Asn Asn 435 440 445Ser Asn Asp Ser Ser Leu
Asp Asn Val Asn Ile Gly Val Ile Asp Asn 450 455 460Ile Pro Glu Ile
Ile Asp Val Asn Pro Tyr Lys Glu Asn Cys Asp Lys465 470 475 480Phe
Ser Pro Val Gln Lys Ile Thr Ser Thr Arg Glu Ile Asn Thr Asn 485 490
495Ile Pro Trp Pro Ile Asn Tyr Leu Gln Ala Gln Asn Thr Asn Asn Glu
500 505 510Lys Phe Ser Leu Ser Ser Asp Phe Val Glu Val Val Ser Ser
Lys Asp 515 520 525Lys Ser Leu Val Tyr Ser Phe Leu Ser Asn Val Met
Phe Tyr Leu Asp 530 535 540Ser Ile Lys Asp Asn Ser Pro Ile Asp Thr
Asp Lys Lys Tyr Tyr Leu545 550 555 560Trp Leu Arg Glu Ile Phe Arg
Asn Tyr Ser Phe Asp Ile Thr Ala Thr 565 570 575Gln Glu Ile Asn Thr
Asp Cys Gly Ile Asn Lys Val Val Thr Trp Phe 580 585 590Gly Lys Ala
Leu Asn Ile Leu Asn Thr Ser Asp Ser Phe Val Glu Glu 595 600 605Phe
Gln Asn Leu Gly Pro Ile Ser Leu Ile Asn Lys Lys Glu Asn Leu 610 615
620Ser Met Pro Lys Ile Glu Ile Asp Glu Ile Pro Asn Ser Met Leu
Asn625 630 635 640Leu Ser Phe Lys Asp Leu Ser Glu Asn Leu Phe Asn
Ile Phe Ser Lys 645 650 655Asn Asn Ser Tyr Phe Glu Lys Ile Tyr Tyr
Asp Phe Leu Asp Gln Trp 660 665 670Trp Thr Gln Tyr Tyr Ser Gln Tyr
Phe Asp Leu Ile Cys Met Ala Lys 675 680 685Arg Ser Val Leu Ala Gln
Glu Ser Leu Ile Lys Lys Ile Ile Gln Lys 690 695 700Lys Leu Ser Tyr
Leu Ile Gly Asn Ser Asn Ile Ser Ser Asp Asn Leu705 710 715 720Ala
Leu Met Asn Leu Thr Thr Thr Asn Thr Leu Arg Asp Ile Ser Asn 725 730
735Glu Ser Gln Ile Ala Met Asn Asn Val Asn Asn Phe Leu Asn Asn Val
740 745 750Ala Ile Cys Val Phe Gln Thr Asn Ile Tyr Pro Lys Phe Ile
Ser Phe 755 760 765Met Glu Gln Cys Ile Asn Asn Ile Asn Lys Asn Thr
Arg Glu Phe Ile 770 775 780Gln Lys Cys Thr Asn Ile Thr Glu Asn Glu
Lys Leu Gln Leu Ile Asn785 790 795 800Gln Asn Ile Phe Ser Ser Leu
Asp Phe Asp Phe Leu Asn Ile Glu Asn 805 810 815Leu Lys Ser Leu Phe
Asn Ser Glu Thr Gly Leu Leu Ile Lys Glu Glu 820 825 830Thr Ser Pro
Tyr Glu Leu Val Leu Tyr Ala Phe Gln Glu Pro Gly Asn 835 840 845Asn
Ala Ile Gly Asp Ala Ser Gly Lys Asn Thr Ser Ile Glu Tyr Ser 850 855
860Lys Asp Ile Gly Leu Val Tyr Gly Ile Asn Ser Asp Ala Leu Tyr
Leu865 870 875 880Asn Gly Ser Asn Gln Ser Ile Ser Phe Ser Asn Asp
Phe Phe Glu Asn 885 890 895Gly Leu Thr Asn Ser Phe Ser Ile Tyr Phe
Trp Leu Arg Asn Leu Gly 900 905 910Lys Asp Thr Ile Lys Ser Lys Leu
Ile Gly Ser Lys Glu Asp Asn Cys 915 920 925Gly Trp Glu Ile Tyr Phe
Gln Asp Thr Gly Leu Val Phe Asn Met Ile 930 935 940Asp Ser Asn Gly
Asn Glu Lys Asn Ile Tyr Leu Ser Asp Val Ser Asn945 950 955 960Asn
Ser Trp His Tyr Ile Thr Ile Ser Val Asp Arg Leu Lys Glu Gln 965 970
975Leu Leu Ile Phe Ile Asp Asp Asn Leu Val Ala Asn Glu Ser Ile Lys
980 985 990Glu Ile Leu Asn Ile Tyr Ser Ser Asn Thr Ile Ser Leu Val
Asn Glu 995 1000 1005Asn Asn Pro Ile Tyr Val Glu Gly Leu Ser Ile
Leu Asn Arg Ser Ile 1010 1015 1020Thr Ser Glu Glu Val Val Asn Asn
Tyr Phe Thr Tyr Leu Asn Asn Ser1025 1030 1035 1040Tyr Ile Arg Asp
Ile Ser Gly Glu Arg Leu Glu Tyr Asn Lys Thr Tyr 1045 1050 1055Glu
Leu Tyr Asn Tyr Val Phe Pro Glu Ser Ser Leu Tyr Glu Val Thr 1060
1065 1070Glu Asn Asn Asn Ile Tyr Leu Ser Ile Lys Asn Thr Asn Asn
Leu Asn 1075 1080 1085Ile Gln Gly Ala Lys Phe Lys Leu Ile Asn Ile
Asp Ala Asn Lys Gln 1090 1095 1100Tyr Val Gln Lys Trp Asp Glu Gly
Val Val Cys Leu Leu Gly Asp Glu1105 1110 1115 1120Glu Lys Tyr Val
Asp Ile Ser Ser Glu Asn Asn Arg Ile Gln Leu Val 1125 1130 1135Ser
Ser Lys Asp Thr Ala Lys Arg Ile Ile Phe Asn Asn Asp Ile Phe 1140
1145 1150Arg Pro Asn Cys Leu Thr Phe Ala Tyr Asn Asn Lys Tyr Leu
Ser Leu 1155 1160 1165Ser Leu Arg Asp Arg Asn Tyr Asn Trp Met Ile
Cys Asn Asn Asn Asp 1170 1175 1180Asn Ile Pro Lys Ala Ala His Leu
Trp Ala Leu Lys Gly Ile1185 1190 1195251197PRTClostridium botulinum
serotype B 25Met Asn Ile Asn Asp Asn Leu Ser Ile Asn Ser Pro Val
Asp Asn Lys1 5 10 15Asn Val Val Val Val Arg Ala Arg Lys Thr Asp Thr
Val Phe Lys Ala 20 25 30Phe Lys Val Ala Pro Asn Ile Trp Val Ala Pro
Glu Arg Tyr Tyr Gly 35 40 45Glu Ser Leu Ser Ile Asp Glu Glu Tyr Lys
Val Asp Gly Gly Ile Tyr 50 55 60Asp Ser Asn Phe Leu Ser Gln Asp Ser
Glu Lys Asp Lys Phe Leu Gln65 70 75 80Ala Ile Ile Thr Leu Leu Lys
Arg Ile Asn Ser Thr Asn Ala Gly Glu 85 90 95Lys Leu Leu Ser Leu Ile
Ser Thr Ala Ile Pro Phe Pro Tyr Gly Tyr 100 105 110Ile Gly Gly Gly
Tyr Tyr Ala Pro Asn Met Ile Thr Phe Gly Ser Ala 115 120 125Pro Lys
Ser Asn Lys Lys Leu Asn Ser Leu Ile Ser Ser Thr Ile Pro 130 135
140Phe Pro Tyr Ala Gly Tyr Arg Glu Thr Asn Tyr Leu Ser Ser Glu
Asp145 150 155 160Asn Lys Ser Phe Tyr Ala Ser Asn Ile Val Ile Phe
Gly Pro Gly Ala 165 170 175Asn Ile Val Glu Asn Asn Thr Val Phe Tyr
Lys Lys Glu Asp Ala Glu 180 185 190Asn Gly Met Gly Thr Met Thr Glu
Ile Trp Phe Gln Pro Phe Leu Thr 195 200 205Tyr Lys Tyr Asp Glu Phe
Tyr Ile Asp Pro Ala Ile Glu Leu Ile Lys 210 215 220Cys Leu Ile Lys
Ser Leu Tyr Phe Leu Tyr Gly Ile Lys Pro Ser Asp225 230 235 240Asp
Leu Val Ile Pro Tyr Arg Leu Arg Ser Glu Leu Glu Asn Ile Glu 245 250
255Tyr Ser Gln Leu Asn Ile Val Asp Leu Leu Val Ser Gly Gly Ile Asp
260 265 270Pro Lys Phe Ile Asn Thr Asp Pro Tyr Trp Phe Thr Asp Asn
Tyr Phe 275 280 285Ser Asn Ala Lys Lys Val Phe Glu Asp His Arg Asn
Ile Tyr Glu Thr 290 295 300Gln Ile Glu Gly Asn Asn Ala Ile Gly Asn
Asp Ile Lys Leu Arg Leu305 310 315 320Lys Gln Lys Phe Arg Ile Asn
Ile Asn Asp Ile Trp Glu Leu Asn Leu 325 330 335Asn Tyr Phe Ser Lys
Glu Phe Ser Ile Met Met Pro Asp Arg Phe Asn 340 345 350Asn Ala Leu
Lys His Phe Tyr Arg Lys Gln Tyr Tyr Lys Ile Asp Tyr 355 360 365Pro
Glu Asn Tyr Ser Ile Asn Gly Phe Val Asn Gly Gln Ile Asn Val 370 375
380Gln Leu Ser Leu Ser Asp Arg Asn Gln Asp Ile Ile Asn Lys Pro
Glu385 390 395 400Glu Ile Ile Asn Leu Leu Asn Gly Asn Asn Val Ser
Leu Met Arg Ser 405 410 415Asn Ile Tyr Gly Asp Gly Leu Lys Ser Thr
Val Asp Asp Phe Tyr Ser 420 425 430Asn Tyr Lys Ile Pro Tyr Asn Arg
Ala Tyr Glu Tyr His Phe Asn Asn 435 440 445Ser Asn Asp Ser Ser Leu
Asp Asn Val Asn Ile Gly Val Ile Asp Asn 450 455 460Ile Pro Glu Ile
Ile Asp Val Asn Pro Tyr Lys Glu Asn Cys Asp Lys465 470 475 480Phe
Ser Pro Val Gln Lys Ile Thr Ser Thr Arg Glu Ile Asn Thr Asn 485 490
495Ile Pro Trp Pro Ile Asn Tyr Leu Gln Ala Gln Asn Thr Asn Asn Glu
500 505 510Lys Phe Ser Leu Ser Ser Asp Phe Val Glu Val Val Ser Ser
Lys Asp 515 520 525Lys Ser Leu Val Tyr Ser Phe Leu Ser Asn Val Met
Phe Tyr Leu Asp 530 535 540Ser Ile Lys Asp Asn Ser Pro Ile Asp Thr
Asp Lys Lys Tyr Tyr Leu545 550 555 560Trp Leu Arg Glu Ile Phe Arg
Asn Tyr Ser Phe Asp Ile Thr Ala Thr 565 570 575Gln Glu Ile Asn Thr
Asp Cys Gly Ile Asn Lys Val Val Thr Trp Phe 580 585 590Gly Lys Ala
Leu Asn Ile Leu Asn Thr Ser Asp Ser Phe Val Glu Glu 595 600 605Phe
Gln Asn Leu Gly Pro Ile Ser Leu Ile Asn Lys Lys Glu Asn Leu 610 615
620Ser Met Pro Ile Ile Glu Ile Tyr Gly Ile Pro Asn Asp Met Leu
Gly625 630 635 640Leu Pro Leu Asn Asp Leu Asn Glu Lys Leu Phe Asn
Ile Tyr Leu Lys 645 650 655Asn Ile Leu Tyr Phe Lys Lys Val Tyr Phe
Asn Phe Leu Asp Gln Trp 660 665 670Trp Thr Glu Tyr Tyr Ser Gln Tyr
Phe Asp Leu Ile Cys Met Ala Lys 675 680 685Gln Ser Ile Leu Ala Gln
Glu Lys Leu Ile Lys Gln Ile Ile Gln Asn 690 695 700Lys Leu Gln Asp
Leu Phe Lys Ala Asp Ile Ser Met Asp Lys Leu Asn705 710 715 720Leu
Met Asn Leu Ala Thr Glu Lys Thr Phe Ile Asp Leu Ser Asn Glu 725 730
735Ser Gln Ile Ala Ile Asn Asn Ile Asn Asp Phe Leu Asn Lys Ser Ala
740 745 750Ile Cys Val Phe Asp Thr Asn Ile Tyr Pro Lys Phe Ile Ser
Phe Met 755 760 765Glu Gln Cys Ile Asn Ser Val Asn Ser Asn Val Thr
Ala Phe Ile Gln 770 775 780Lys Cys Thr Asn Ile Thr Glu Asp Glu Lys
Leu Gln Leu Ile Lys Leu785 790 795 800Asn Thr Phe Met Asn Ile Asp
Phe Glu Phe Phe Asp Ile Gln Ser Ile 805 810 815Lys Asp Leu Ile Thr
Ser Glu Thr Asp Leu Ile Lys Glu Glu Lys Glu 820 825 830Ser Asp Tyr
Asn Leu Phe Leu Phe Thr Leu Gln Glu Asp Asn Asn Lys 835 840 845Val
Ile Glu Asp Ile Ser Gly Lys Asn Thr Leu Val Lys Tyr Ser Asp 850 855
860Ser Ile Ser Leu Val Tyr Gly Val Asn Gly Asp Ala Leu Tyr Leu
Lys865 870 875 880Glu Pro Asp Glu Ser Val Ser Phe Ser Asn Lys Ala
Phe Glu Asn Gly 885 890 895Leu Thr Asn Ser Phe Ser Ile Cys Phe Trp
Leu Arg Asn Leu Gly Glu 900 905 910Asp Ile Ile Thr Ser Lys Leu Ile
Glu Asn Lys Ala Asp Asn Cys Gly 915 920 925Trp Glu Ile Tyr Phe Glu
Asn Asn Gly Leu Val Phe Ser Ile Val Asp 930 935 940Cys Asn Gly Asn
Glu Glu Asn Ile Tyr Leu Ser Asp Val Ile Ser Lys945 950 955 960Asn
Trp Tyr Tyr Ile Ser Ile Ser Ile Asp Arg Leu Arg Asn Gln Leu 965 970
975Leu Ile Phe Ile Asn Asp Lys Leu Ile Ala Asn Gln Ser Ile Glu Gln
980 985 990Ile Leu Asn Ile Tyr Ser Ser Asn Thr Ile Ser Leu Val Asn
Glu Asn 995 1000
1005Asn Pro Ile Tyr Ile Glu Gly Leu Ser Ile Leu Asn Arg Ser Ile Thr
1010 1015 1020Ser Glu Glu Val Val Asn Asn Tyr Phe Ser Tyr Leu Asn
Asn Ser Tyr1025 1030 1035 1040Ile Arg Asp Ile Ser Gly Glu Arg Leu
Glu Tyr Asn Lys Thr Tyr Glu 1045 1050 1055Leu Tyr Asn Tyr Val Phe
Pro Glu Asn Ser Leu Tyr Glu Val Thr Glu 1060 1065 1070Asn Asn Asn
Ile Tyr Leu Ser Ile Lys Asp Thr Asn Asn Leu Asn Ile 1075 1080
1085Gln Gly Ala Lys Phe Lys Leu Ile Asn Ile Asp Ala Asn Lys Gln Tyr
1090 1095 1100Val Gln Lys Trp Asp Glu Gly Val Val Cys Leu Leu Gly
Asp Glu Glu1105 1110 1115 1120Lys Tyr Val Asp Ile Ser Ser Glu Asn
Asn Arg Ile Gln Leu Val Asn 1125 1130 1135Ser Lys Asp Thr Ala Lys
Arg Ile Ile Phe Asn Asn Asp Ile Phe Met 1140 1145 1150Pro Asn Cys
Leu Thr Phe Ala Tyr Asn Asn Lys Tyr Leu Ser Leu Ser 1155 1160
1165Leu Arg Asp Arg Asn Tyr Asn Trp Met Ile Cys Asn Asn Asn Asp Asn
1170 1175 1180Ile Pro Lys Ala Ala His Leu Trp Ala Leu Lys Gly
Ile1185 1190 1195261193PRTClostridium botulinum serotype B 26Met
Asp Ile Asn Asp Asp Leu Asn Ile Asn Ser Pro Val Asp Asn Lys1 5 10
15Asn Val Val Ile Val Arg Ala Arg Lys Thr Asn Thr Phe Phe Lys Ala
20 25 30Phe Lys Val Ala Pro Asn Ile Trp Val Ala Pro Glu Arg Tyr Tyr
Gly 35 40 45Glu Pro Leu His Ile Ala Glu Glu Tyr Lys Leu Asp Gly Gly
Ile Tyr 50 55 60Asp Ser Asn Phe Leu Ser Gln Asp Ser Glu Arg Glu Asn
Phe Leu Gln65 70 75 80Ala Ile Ile Ile Leu Leu Lys Arg Ile Asn Asn
Thr Ile Ser Gly Lys 85 90 95Gln Leu Leu Ser Leu Ile Ser Thr Ala Ile
Pro Phe Pro Tyr Gly Tyr 100 105 110Ile Gly Gly Gly Tyr Ser Ser Pro
Asn Ile Phe Thr Phe Gly Lys Thr 115 120 125Pro Arg Thr Asn Lys Lys
Leu Asn Ser Leu Val Thr Ser Thr Ile Pro 130 135 140Phe Pro Phe Gly
Gly Tyr Arg Glu Thr Asn Tyr Ile Glu Ser Gln Asn145 150 155 160Asn
Lys Asn Phe Tyr Ala Ser Asn Ile Ile Ile Phe Gly Pro Gly Ser 165 170
175Asn Ile Val Glu Asn Asn Val Ile Tyr Tyr Lys Lys Asn Asp Ala Glu
180 185 190Asn Gly Met Gly Thr Met Ala Glu Ile Val Phe Gln Pro Leu
Leu Thr 195 200 205Tyr Lys Tyr Asn Lys Phe Tyr Ile Asp Pro Ala Met
Glu Leu Thr Lys 210 215 220Cys Leu Ile Lys Ser Leu Tyr Phe Leu Tyr
Gly Ile Lys Pro Ser Gly225 230 235 240Asn Leu Val Val Pro Tyr Arg
Leu Arg Thr Glu Leu Asp Asn Lys Gln 245 250 255Phe Ser Gln Leu Asn
Ile Ile Asp Leu Leu Ile Ser Gly Gly Val Asp 260 265 270Leu Glu Phe
Ile Asn Thr Asn Pro Tyr Trp Phe Thr Asn Ser Tyr Phe 275 280 285Pro
Asn Ser Ile Lys Met Phe Glu Lys Tyr Lys Asn Ile Tyr Lys Thr 290 295
300Glu Ile Glu Gly Asn Asn Ala Ile Gly Asn Asp Ile Lys Leu Arg
Leu305 310 315 320Lys Gln Lys Phe Gln Ile Asn Val Gln Asp Ile Trp
Asn Leu Asn Leu 325 330 335Asn Tyr Phe Cys Gln Ser Phe Asn Ser Ile
Ile Pro Asp Arg Phe Ser 340 345 350Asn Ala Leu Lys His Phe Tyr Arg
Lys Gln Tyr Tyr Thr Met Asp Tyr 355 360 365Thr Asp Asn Tyr Asn Ile
Asn Gly Phe Val Asn Gly Gln Ile Asn Thr 370 375 380Lys Leu Pro Leu
Ser Asn Lys Asn Thr Asn Ile Ile Ser Lys Pro Glu385 390 395 400Lys
Val Val Asn Leu Val Asn Glu Asn Asn Ile Ser Leu Met Lys Ser 405 410
415Asn Ile Tyr Gly Asp Gly Leu Lys Gly Thr Thr Glu Asp Phe Tyr Ser
420 425 430Thr Tyr Lys Ile Pro Tyr Asp Glu Glu Tyr Glu Tyr Arg Phe
Asn Asp 435 440 445Ser Asp Asn Phe Pro Leu Asn Asn Ile Ser Ile Glu
Glu Val Asp Ser 450 455 460Ile Pro Glu Ile Ile Asp Ile Asn Pro Tyr
Lys Asp Asn Ser Asp Asn465 470 475 480Leu Val Phe Thr Gln Ile Thr
Ser Met Thr Glu Glu Val Thr Thr His 485 490 495Thr Ala Leu Ser Ile
Asn Tyr Leu Gln Ala Gln Ile Thr Asn Asn Glu 500 505 510Asn Phe Thr
Leu Ser Ser Asp Phe Ser Lys Val Val Ser Ser Lys Asp 515 520 525Lys
Ser Leu Val Tyr Ser Phe Leu Asp Asn Leu Met Ser Tyr Leu Glu 530 535
540Thr Ile Lys Asn Asp Arg Pro Ile His Thr Asp Lys Lys Tyr Tyr
Leu545 550 555 560Trp Leu Lys Glu Val Phe Lys Asn Tyr Ser Phe Asp
Ile Asn Leu Thr 565 570 575Gln Glu Ile Asp Ser Met Cys Gly Ile Asn
Gln Val Val Leu Trp Phe 580 585 590Gly Lys Ala Leu Asn Ile Leu Asn
Thr Ser Asn Ser Phe Val Glu Glu 595 600 605Tyr Gln Asp Ser Gly Ala
Ile Ser Leu Ile Ser Lys Lys Asp Asn Leu 610 615 620Arg Glu Pro Asn
Ile Glu Ile Asp Asp Ile Ser Asp Ser Leu Leu Gly625 630 635 640Leu
Ser Phe Lys Asp Leu Asn Asn Lys Leu Tyr Glu Ile Tyr Ser Lys 645 650
655Asn Ile Val Tyr Phe Lys Lys Ile Tyr Phe Ser Phe Leu Asp Gln Trp
660 665 670Trp Thr Gln Tyr Tyr Ser Gln Tyr Phe Asp Leu Ile Cys Met
Ala Lys 675 680 685Lys Ser Ile Leu Ala Gln Glu Thr Leu Ile Lys Lys
Ile Ile Gln Lys 690 695 700Lys Leu Ser Tyr Leu Ile Gly Asn Ser Asn
Ile Ser Ser Asp Asn Leu705 710 715 720Ala Leu Met Asn Leu Thr Thr
Thr Asn Thr Leu Arg Asp Ile Ser Asn 725 730 735Glu Ser Gln Ile Ala
Met Asn Asn Val Asp Ser Phe Leu Asn Ser Ala 740 745 750Ala Ile Cys
Val Phe Glu Gly Asn Ile Tyr Pro Lys Phe Ile Ser Phe 755 760 765Met
Glu Gln Cys Ile Asn Asn Ile Asn Lys Asn Thr Arg Glu Phe Ile 770 775
780Gln Lys Cys Thr Asn Ile Thr Glu Asn Glu Lys Leu Gln Leu Ile
Asn785 790 795 800Gln Asn Ile Phe Ser Ser Leu Asp Phe Asp Phe Leu
Asn Ile Glu Asn 805 810 815Leu Lys Ser Leu Phe Ser Ser Glu Thr Ala
Leu Leu Ile Lys Glu Glu 820 825 830Thr Ser Pro Tyr Glu Leu Val Leu
Tyr Ala Phe Gln Glu Pro Asp Asn 835 840 845Asn Ala Ile Gly Asp Ala
Ser Ala Lys Asn Thr Ser Ile Glu Tyr Ser 850 855 860Lys Asp Ile Asp
Leu Val Tyr Gly Ile Asn Ser Asp Ala Leu Tyr Leu865 870 875 880Asn
Gly Ser Asn Gln Ser Ile Ser Phe Ser Asn Asp Phe Phe Glu Asn 885 890
895Gly Leu Thr Asn Ser Phe Ser Ile Tyr Phe Trp Leu Arg Asn Leu Gly
900 905 910Lys Asp Thr Ile Lys Ser Lys Leu Ile Gly Ser Lys Gly Asp
Asn Cys 915 920 925Gly Trp Glu Ile Tyr Phe Gln Asp Thr Gly Leu Val
Phe Asn Met Ile 930 935 940Asp Ser Asn Gly Asn Glu Lys Asn Ile Tyr
Leu Ser Asp Val Ser Asn945 950 955 960Asn Ser Trp His Tyr Ile Thr
Ile Ser Val Asp Arg Leu Lys Glu Gln 965 970 975Leu Leu Ile Phe Ile
Asp Asp Asn Leu Val Ala Asn Glu Ser Ile Lys 980 985 990Glu Ile Leu
Asn Ile Tyr Ser Ser Asn Ile Ile Ser Leu Leu Ser Glu 995 1000
1005Asn Asn Pro Ser Tyr Ile Glu Gly Leu Thr Ile Leu Asn Lys Pro Thr
1010 1015 1020Thr Ser Gln Glu Val Leu Asn Asn Tyr Phe Lys Val Leu
Asn Asn Ser1025 1030 1035 1040Tyr Ile Arg Asp Ser Asn Glu Glu Arg
Leu Glu Tyr Asn Lys Thr Tyr 1045 1050 1055Gln Leu Tyr Asn Tyr Val
Phe Ser Asp Lys Pro Ile Cys Glu Val Lys 1060 1065 1070Gln Asn Asn
Asn Ile Tyr Leu Thr Ile Asn Asn Thr Asn Asn Leu Asn 1075 1080
1085Leu Gln Pro Ser Lys Phe Lys Leu Leu Ser Ile Asn Pro Asn Lys Gln
1090 1095 1100Tyr Val Gln Lys Leu Asp Glu Val Ile Ile Ser Val Leu
Gly Asn Met1105 1110 1115 1120Glu Lys Tyr Ile Asp Ile Ser Glu Asp
Asn Arg Leu Gln Leu Ile Asp 1125 1130 1135Asn Lys Asn Gly Ala Lys
Lys Met Ile Ile Ser Asn Asp Met Phe Ile 1140 1145 1150Ser Asn Cys
Leu Thr Leu Ser Cys Gly Gly Lys Tyr Ile Cys Leu Ser 1155 1160
1165Met Lys Asp Glu Asn His Asn Trp Met Ile Cys Asn Asn Asp Met Ser
1170 1175 1180Lys Tyr Leu Tyr Leu Trp Ser Phe Lys1185
1190271196PRTClostridium botulinum serotype C1 27Met Asp Ile Asn
Asp Asp Leu Asn Ile Asn Ser Pro Val Asp Asn Lys1 5 10 15Asn Val Val
Ile Val Arg Ala Arg Lys Thr Asn Thr Phe Phe Lys Ala 20 25 30Phe Lys
Val Ala Pro Asn Ile Trp Val Ala Pro Glu Arg Tyr Tyr Gly 35 40 45Glu
Pro Leu Asp Ile Ala Glu Glu Tyr Lys Leu Asp Gly Gly Ile Tyr 50 55
60Asp Ser Asn Phe Leu Ser Gln Asp Ser Glu Arg Glu Asn Phe Leu Gln65
70 75 80Ala Ile Ile Ile Leu Leu Lys Arg Ile Asn Asn Thr Ile Ser Gly
Lys 85 90 95Gln Leu Leu Ser Leu Ile Ser Thr Ala Ile Pro Phe Pro Tyr
Gly Tyr 100 105 110Ile Gly Gly Gly Tyr Ser Ser Pro Asn Ile Phe Thr
Phe Gly Lys Thr 115 120 125Pro Lys Ser Asn Lys Lys Leu Asn Ser Leu
Val Thr Ser Thr Ile Pro 130 135 140Phe Pro Phe Gly Gly Tyr Arg Glu
Thr Asn Tyr Ile Glu Ser Gln Asn145 150 155 160Asn Lys Asn Phe Tyr
Ala Ser Asn Ile Ile Ile Phe Gly Pro Gly Ser 165 170 175Asn Ile Val
Glu Asn Asn Val Ile Tyr Tyr Lys Lys Asn Asp Ala Glu 180 185 190Asn
Gly Met Gly Thr Met Ala Glu Ile Val Phe Gln Pro Leu Leu Thr 195 200
205Tyr Lys Tyr Asn Lys Phe Tyr Ile Asp Pro Ala Met Glu Leu Thr Lys
210 215 220Cys Leu Ile Lys Ser Leu Tyr Phe Leu Tyr Gly Ile Lys Pro
Ser Asp225 230 235 240Asn Leu Val Val Pro Tyr Arg Leu Arg Thr Glu
Leu Asp Asn Lys Gln 245 250 255Phe Ser Gln Leu Asn Ile Ile Asp Leu
Leu Ile Ser Gly Gly Val Asp 260 265 270Leu Glu Phe Ile Asn Thr Asn
Pro Tyr Trp Phe Thr Asn Ser Tyr Phe 275 280 285Pro Asn Ser Ile Lys
Met Phe Glu Lys Tyr Lys Asn Ile Tyr Lys Thr 290 295 300Glu Ile Glu
Gly Asn Asn Ala Ile Gly Asn Asp Ile Lys Leu Arg Leu305 310 315
320Lys Gln Lys Phe Gln Ile Asn Val Gln Asp Ile Trp Asn Leu Asn Leu
325 330 335Asn Tyr Phe Cys Gln Ser Phe Asn Ser Ile Ile Pro Asp Arg
Phe Ser 340 345 350Asn Ala Leu Lys His Phe Tyr Arg Lys Gln Tyr Tyr
Thr Met Asp Tyr 355 360 365Thr Asp Asn Tyr Asn Ile Asn Gly Phe Val
Asn Gly Gln Ile Asn Thr 370 375 380Lys Leu Pro Leu Ser Asn Lys Asn
Thr Asn Ile Ile Ser Lys Pro Glu385 390 395 400Lys Val Val Asn Leu
Val Asn Glu Asn Asn Ile Ser Leu Met Lys Ser 405 410 415Asn Ile Tyr
Gly Asp Gly Leu Lys Gly Thr Thr Glu Asp Phe Tyr Ser 420 425 430Thr
Tyr Lys Ile Pro Tyr Asn Glu Glu Tyr Glu Tyr Arg Phe Asn Asp 435 440
445Ser Asp Asn Phe Pro Leu Asn Asn Ile Ser Ile Glu Glu Val Asp Ser
450 455 460Ile Pro Glu Ile Ile Asp Ile Asn Pro Tyr Lys Asp Asn Ser
Asp Asn465 470 475 480Leu Val Phe Thr Gln Ile Thr Ser Met Thr Glu
Glu Val Thr Thr His 485 490 495Thr Ala Leu Ser Ile Asn Tyr Leu Gln
Ala Gln Ile Thr Asn Asn Glu 500 505 510Asn Phe Thr Leu Ser Ser Asp
Phe Ser Lys Val Val Ser Ser Lys Asp 515 520 525Lys Ser Leu Val Tyr
Ser Phe Leu Asp Asn Leu Met Ser Tyr Leu Glu 530 535 540Thr Ile Lys
Asn Asp Gly Pro Ile Asp Thr Asp Lys Lys Tyr Tyr Leu545 550 555
560Trp Leu Lys Glu Val Phe Lys Asn Tyr Ser Phe Asp Ile Asn Leu Thr
565 570 575Gln Glu Ile Asp Ser Met Cys Gly Ile Asn Glu Val Val Leu
Trp Phe 580 585 590Gly Lys Ala Leu Asn Ile Leu Asn Thr Ser Asn Ser
Phe Val Glu Glu 595 600 605Tyr Gln Asp Ser Gly Ala Ile Ser Leu Ile
Ser Lys Lys Asp Asn Leu 610 615 620Arg Glu Pro Asn Ile Glu Ile Asp
Asp Ile Ser Asp Ser Leu Leu Gly625 630 635 640Leu Ser Phe Lys Asp
Leu Asn Asn Lys Leu Tyr Glu Ile Tyr Ser Lys 645 650 655Asn Ile Val
Tyr Phe Lys Lys Ile Tyr Phe Ser Phe Leu Asp Gln Trp 660 665 670Trp
Thr Glu Tyr Tyr Ser Gln Tyr Phe Glu Leu Ile Cys Met Ala Lys 675 680
685Gln Ser Ile Leu Ala Gln Glu Ser Leu Val Lys Gln Ile Val Gln Asn
690 695 700Lys Phe Thr Asp Leu Ser Lys Ala Ser Ile Pro Pro Asp Thr
Leu Lys705 710 715 720Leu Ile Arg Glu Thr Thr Glu Lys Thr Phe Ile
Asp Leu Ser Asn Glu 725 730 735Ser Gln Ile Ser Met Asn Arg Val Asp
Asn Phe Leu Asn Lys Ala Ser 740 745 750Ile Cys Val Phe Val Glu Asp
Ile Tyr Pro Lys Phe Ile Ser Tyr Met 755 760 765Glu Lys Tyr Ile Asn
Asn Ile Asn Ile Lys Thr Arg Glu Phe Ile Gln 770 775 780Arg Cys Thr
Asn Ile Asn Asp Asn Glu Lys Ser Ile Leu Ile Asn Ser785 790 795
800Tyr Thr Phe Lys Thr Ile Asp Phe Lys Phe Leu Asp Ile Gln Ser Ile
805 810 815Lys Asn Phe Phe Asn Ser Gln Val Glu Gln Val Met Lys Glu
Ile Leu 820 825 830Ser Pro Tyr Gln Leu Leu Leu Phe Ala Ser Lys Gly
Pro Asn Ser Asn 835 840 845Ile Ile Glu Asp Ile Ser Gly Lys Asn Thr
Leu Ile Gln Tyr Thr Glu 850 855 860Ser Ile Glu Leu Val Tyr Gly Val
Asn Gly Glu Ser Leu Tyr Leu Lys865 870 875 880Ser Pro Asn Glu Thr
Ile Lys Phe Ser Asn Lys Phe Phe Thr Asn Gly 885 890 895Leu Thr Asn
Asn Phe Thr Ile Cys Phe Trp Leu Arg Phe Thr Gly Lys 900 905 910Asn
Asp Asp Lys Thr Arg Leu Ile Gly Asn Lys Val Asn Asn Cys Gly 915 920
925Trp Glu Ile Tyr Phe Glu Asp Asn Gly Leu Val Phe Glu Ile Ile Asp
930 935 940Ser Asn Gly Asn Gln Glu Ser Val Tyr Leu Ser Asn Ile Ile
Asn Asp945 950 955 960Asn Trp Tyr Tyr Ile Ser Ile Ser Val Asp Arg
Leu Lys Asp Gln Leu 965 970 975Leu Ile Phe Ile Asn Asp Lys Asn Val
Ala Asn Val Ser Ile Asp Gln 980 985 990Ile Leu Ser Ile Tyr Ser Thr
Asn Ile Ile Ser Leu Val Asn Lys Asn 995 1000 1005Asn Ser Ile Tyr
Val Glu Glu Leu Ser Val Leu Asp Asn Pro Ile Thr 1010 1015 1020Ser
Glu Glu Val Ile Arg Asn Tyr Phe Ser Tyr Leu Asp Asn Ser Tyr1025
1030 1035 1040Ile Arg Asp Ser Ser Lys Ser Leu Leu Glu Tyr Asn Lys
Asn Tyr Gln 1045 1050 1055Leu Tyr Asn Tyr Val Phe Pro Glu Thr Ser
Leu Tyr Glu Val Asn Asp 1060 1065 1070Asn Asn Lys Ser Tyr Leu
Ser Leu Lys Asn Thr Asp Gly Ile Asn Ile 1075 1080 1085Ser Ser Val
Lys Phe Lys Leu Ile Asn Ile Asp Glu Ser Lys Val Tyr 1090 1095
1100Val Gln Lys Trp Asp Glu Cys Ile Ile Cys Val Leu Asp Gly Thr
Glu1105 1110 1115 1120Lys Tyr Leu Asp Ile Ser Pro Glu Asn Asn Arg
Ile Gln Leu Val Ser 1125 1130 1135Ser Lys Asp Asn Ala Lys Lys Ile
Thr Val Asn Thr Asp Leu Phe Arg 1140 1145 1150Pro Asp Cys Ile Thr
Phe Ser Tyr Asn Asp Lys Tyr Phe Ser Leu Ser 1155 1160 1165Leu Arg
Asp Gly Asp Tyr Asn Trp Met Ile Cys Asn Asp Asn Asn Lys 1170 1175
1180Val Pro Lys Gly Ala His Leu Trp Ile Leu Glu Ser1185 1190
1195281196PRTClostridium botulinum serotype D 28Met Asp Ile Asn Asp
Asp Leu Asn Ile Asn Ser Pro Val Asp Asn Lys1 5 10 15Asn Val Val Ile
Val Arg Ala Arg Lys Thr Asn Thr Phe Phe Lys Ala 20 25 30Phe Lys Val
Ala Pro Asn Ile Trp Val Ala Pro Glu Arg Tyr Tyr Gly 35 40 45Glu Pro
Leu Asp Ile Ala Glu Glu Tyr Lys Leu Asp Gly Gly Ile Tyr 50 55 60Asp
Ser Asn Phe Leu Ser Gln Asp Ser Glu Arg Glu Asn Phe Leu Gln65 70 75
80Ala Ile Ile Thr Leu Leu Lys Arg Ile Asn Asn Thr Ile Ser Gly Lys
85 90 95Gln Leu Leu Ser Leu Ile Ser Thr Ala Ile Pro Phe Pro Tyr Gly
Tyr 100 105 110Val Gly Gly Gly Tyr Ser Ser Pro Asn Ile Phe Thr Phe
Gly Lys Thr 115 120 125Pro Lys Ser Asn Lys Lys Leu Asn Ser Leu Val
Thr Ser Thr Ile Pro 130 135 140Phe Pro Phe Gly Gly Tyr Arg Glu Thr
Asn Tyr Ile Glu Ser Gln Asn145 150 155 160Asn Lys Asn Phe Tyr Ala
Ser Asn Ile Val Ile Phe Gly Pro Gly Ser 165 170 175Asn Ile Val Glu
Asn Asn Val Ile Cys Tyr Lys Lys Asn Asp Ala Glu 180 185 190Asn Gly
Met Gly Thr Met Ala Glu Ile Leu Phe Gln Pro Leu Leu Thr 195 200
205Tyr Lys Tyr Asn Lys Phe Tyr Ile Asp Pro Ala Met Glu Leu Thr Lys
210 215 220Cys Leu Ile Lys Ser Leu Tyr Phe Leu Tyr Gly Ile Lys Pro
Ser Asp225 230 235 240Asp Leu Val Val Pro Tyr Arg Leu Arg Thr Glu
Leu Asp Asn Lys Gln 245 250 255Phe Ser Gln Leu Asn Ile Ile Asp Leu
Leu Ile Ser Gly Gly Val Asp 260 265 270Leu Glu Phe Ile Asn Thr Asn
Pro Tyr Trp Phe Thr Asn Ser Tyr Phe 275 280 285Ser Asn Ser Ile Lys
Met Phe Glu Lys Tyr Lys Asn Ile Tyr Glu Thr 290 295 300Glu Ile Glu
Gly Asn Asn Ala Ile Gly Asn Asp Ile Lys Leu Arg Leu305 310 315
320Lys Gln Lys Phe Gln Asn Ser Val Gln Asp Ile Trp Asn Leu Asn Leu
325 330 335Asn Tyr Phe Ser Lys Glu Phe Asn Ser Ile Ile Pro Asp Arg
Phe Ser 340 345 350Asn Ala Leu Lys His Phe Tyr Arg Lys Gln Tyr Tyr
Thr Met Asp Tyr 355 360 365Gly Asp Asn Tyr Asn Ile Asn Gly Phe Val
Asn Gly Gln Ile Asn Thr 370 375 380Lys Leu Pro Leu Ser Asp Lys Asn
Thr Asn Ile Ile Ser Lys Pro Glu385 390 395 400Lys Val Val Asn Leu
Val Asn Ala Asn Asn Ile Ser Leu Met Lys Ser 405 410 415Asn Ile Tyr
Gly Asp Gly Leu Lys Gly Thr Thr Glu Asp Phe Tyr Ser 420 425 430Thr
Tyr Lys Ile Pro Tyr Asn Glu Glu Tyr Glu Tyr Arg Phe Asn Asp 435 440
445Ser Asp Asn Phe Pro Leu Asn Asn Ile Ser Ile Glu Glu Val Asp Ser
450 455 460Ile Pro Glu Ile Ile Asp Ile Asn Pro Tyr Lys Asp Asn Ser
Asp Asp465 470 475 480Leu Leu Phe Thr Gln Ile Thr Ser Thr Thr Glu
Glu Val Ile Thr His 485 490 495Thr Ala Leu Pro Val Asn Tyr Leu Gln
Ala Gln Ile Ile Thr Asn Glu 500 505 510Asn Phe Thr Leu Ser Ser Asp
Phe Ser Lys Val Val Ser Ser Lys Asp 515 520 525Lys Ser Leu Val Tyr
Ser Phe Leu Asp Asn Leu Met Ser Tyr Leu Glu 530 535 540Thr Ile Lys
Asn Asp Gly Pro Ile Asp Thr Asp Lys Lys Tyr Tyr Leu545 550 555
560Trp Leu Lys Glu Val Phe Lys Asn Tyr Ser Phe Asp Ile Asn Leu Thr
565 570 575Gln Glu Ile Asp Ser Ser Cys Gly Ile Asn Glu Val Val Ile
Trp Phe 580 585 590Gly Lys Ala Leu Asn Ile Leu Asn Thr Ser Asn Ser
Phe Val Glu Glu 595 600 605Tyr Gln Asn Ser Gly Pro Ile Ser Leu Ile
Ser Lys Lys Asp Asn Leu 610 615 620Ser Glu Pro Asn Ile Glu Ile Asp
Asp Ile Pro Asp Ser Leu Leu Gly625 630 635 640Leu Ser Phe Lys Asp
Leu Asn Asn Lys Leu Tyr Glu Ile Tyr Ser Lys 645 650 655Asn Arg Val
Tyr Phe Arg Lys Ile Tyr Phe Asn Phe Leu Asp Gln Trp 660 665 670Trp
Thr Glu Tyr Tyr Ser Gln Tyr Phe Glu Leu Ile Cys Met Ala Lys 675 680
685Gln Ser Ile Leu Ala Gln Glu Ser Val Val Lys Gln Ile Ile Gln Asn
690 695 700Lys Phe Thr Asp Leu Ser Lys Ala Ser Ile Pro Pro Asp Thr
Leu Lys705 710 715 720Leu Ile Lys Glu Thr Thr Glu Lys Thr Phe Ile
Asp Leu Ser Asn Glu 725 730 735Ser Gln Ile Ser Met Asn Arg Val Asp
Asn Phe Leu Asn Lys Ala Ser 740 745 750Ile Cys Val Phe Val Glu Asp
Ile Tyr Pro Lys Phe Ile Ser Tyr Met 755 760 765Glu Lys Tyr Ile Asn
Asn Ile Asn Ile Lys Thr Arg Glu Phe Ile Gln 770 775 780Arg Cys Thr
Asn Ile Asn Asp Asn Glu Lys Ser Ile Leu Ile Asn Ser785 790 795
800Tyr Thr Phe Lys Thr Ile Asp Phe Lys Phe Leu Asn Ile Gln Ala Ile
805 810 815Lys Asn Phe Phe Asn Ser Gln Val Glu Gln Val Met Lys Glu
Met Leu 820 825 830Ser Pro Tyr Gln Leu Leu Leu Phe Ala Thr Arg Gly
Pro Asn Ser Asn 835 840 845Ile Ile Glu Asp Ile Ser Gly Lys Asn Thr
Leu Ile Gln Tyr Thr Glu 850 855 860Ser Val Glu Leu Val Tyr Gly Val
Asn Gly Glu Ser Leu Tyr Leu Lys865 870 875 880Ser Pro Asn Glu Thr
Val Glu Phe Ser Asn Asn Phe Phe Thr Asn Gly 885 890 895Leu Thr Asn
Asn Phe Thr Ile Cys Phe Trp Leu Arg Phe Thr Gly Lys 900 905 910Asp
Asp Asp Lys Thr Arg Leu Ile Gly Asn Lys Val Asn Asn Cys Gly 915 920
925Trp Glu Ile Tyr Phe Glu Asp Asn Gly Leu Val Phe Glu Ile Ile Asp
930 935 940Ser Asn Gly Asn Gln Glu Ser Val Tyr Leu Ser Asn Val Ile
Asn Asn945 950 955 960Asn Trp Tyr Tyr Ile Ser Ile Ser Val Asp Arg
Leu Lys Asp Gln Leu 965 970 975Leu Ile Phe Ile Asn Asp Lys Asn Val
Ala Asn Val Ser Ile Glu Gln 980 985 990Ile Leu Asn Ile Tyr Ser Thr
Asn Val Ile Ser Leu Val Asn Lys Asn 995 1000 1005Asn Ser Ile Tyr
Val Glu Glu Leu Ser Val Leu Asp Lys Pro Val Ala 1010 1015 1020Ser
Glu Glu Val Ile Arg Asn Tyr Phe Ser Tyr Leu Asp Asn Ser Tyr1025
1030 1035 1040Ile Arg Asp Ser Ser Lys Ser Leu Leu Glu Tyr Asn Lys
Asn Tyr Gln 1045 1050 1055Leu Tyr Asn Tyr Val Phe Pro Glu Thr Ser
Leu Tyr Glu Val Asn Asp 1060 1065 1070Asn Asn Lys Ser Tyr Leu Ser
Leu Lys Asn Thr Asp Gly Ile Asn Ile 1075 1080 1085Pro Ser Val Lys
Phe Lys Leu Ile Asn Ile Asp Glu Ser Lys Gly Tyr 1090 1095 1100Val
Gln Lys Trp Asp Glu Cys Ile Ile Cys Val Ser Asp Gly Thr Glu1105
1110 1115 1120Lys Tyr Leu Asp Ile Ser Pro Glu Asn Asn Arg Ile Gln
Leu Val Ser 1125 1130 1135Ser Lys Asp Asn Ala Lys Lys Ile Thr Val
Asn Thr Asp Leu Phe Arg 1140 1145 1150Pro Asp Cys Ile Thr Phe Ser
Tyr Asn Asp Lys Tyr Phe Ser Leu Ser 1155 1160 1165Leu Arg Asp Gly
Asp Tyr Asn Trp Met Ile Cys Asn Asp Asn Asn Lys 1170 1175 1180Val
Pro Lys Gly Ala His Leu Trp Ile Leu Lys Ser1185 1190
1195291162PRTClostridium botulinum serotype E 29Met Lys Ile Asn Gly
Asn Leu Asn Ile Asp Ser Pro Val Asp Asn Lys1 5 10 15Asn Val Ala Ile
Val Arg Ser Arg Asn Gln Met Phe Phe Lys Ala Phe 20 25 30Gln Val Ala
Pro Asn Ile Trp Ile Val Pro Glu Arg Tyr Tyr Gly Glu 35 40 45Ser Leu
Lys Ile Asn Glu Asp Gln Lys Phe Asp Gly Gly Ile Tyr Asp 50 55 60Ser
Asn Phe Leu Ser Thr Asn Asn Glu Lys Asp Asp Phe Leu Gln Ala65 70 75
80Thr Ile Lys Leu Leu Gln Arg Ile Asn Asn Asn Val Val Gly Ala Lys
85 90 95Leu Leu Ser Leu Ile Ser Thr Ala Ile Pro Phe Pro Tyr Glu Asn
Asn 100 105 110Thr Glu Asp Tyr Arg Gln Thr Asn Tyr Leu Ser Ser Lys
Asn Asn Glu 115 120 125His Tyr Tyr Thr Ala Asn Leu Val Ile Phe Gly
Pro Gly Ser Asn Ile 130 135 140Ile Lys Asn Asn Val Ile Tyr Tyr Lys
Lys Glu Tyr Ala Glu Ser Gly145 150 155 160Met Gly Thr Met Leu Glu
Ile Trp Phe Gln Pro Phe Leu Thr His Lys 165 170 175Tyr Asp Glu Phe
Tyr Val Asp Pro Ala Leu Glu Leu Ile Lys Cys Leu 180 185 190Ile Lys
Ser Leu Tyr Tyr Leu Tyr Gly Ile Lys Pro Asn Asp Asn Leu 195 200
205Asn Ile Pro Tyr Arg Leu Arg Asn Glu Phe Asn Ser Leu Glu Tyr Ser
210 215 220Glu Leu Asn Met Ile Asp Phe Leu Ile Ser Gly Gly Ile Asp
Tyr Lys225 230 235 240Leu Leu Asn Thr Asn Pro Tyr Trp Phe Ile Asp
Lys Tyr Phe Ile Asp 245 250 255Thr Ser Lys Asn Phe Glu Lys Tyr Lys
Asn Asp Tyr Glu Ile Lys Ile 260 265 270Lys Asn Asn Asn Tyr Ile Ala
Asn Ser Ile Lys Leu Tyr Leu Glu Gln 275 280 285Lys Phe Lys Ile Asn
Val Lys Asp Ile Trp Glu Leu Asn Leu Ser Tyr 290 295 300Phe Ser Lys
Glu Phe Gln Ile Met Met Pro Glu Arg Tyr Asn Asn Ala305 310 315
320Leu Asn His Tyr Tyr Arg Lys Glu Phe Tyr Val Ile Asp Tyr Phe Lys
325 330 335Asn Tyr Asn Ile Asn Gly Phe Lys Asn Gly Gln Ile Lys Thr
Lys Leu 340 345 350Pro Leu Ser Lys Tyr Asn Lys Glu Ile Ile Asn Lys
Pro Glu Leu Ile 355 360 365Val Asn Leu Ile Asn Gln Asn Asn Thr Val
Leu Met Lys Ser Asn Ile 370 375 380Tyr Gly Asp Gly Leu Lys Gly Thr
Val Asp Asn Phe Tyr Ser Asn Tyr385 390 395 400Ile Ile Pro Tyr Asn
Leu Asn Tyr Glu His Ser Ile Asn Tyr Phe Tyr 405 410 415Leu Asp Asn
Val Asn Ile Glu Glu Ile Glu Lys Ile Pro Pro Ile Asn 420 425 430Asp
Glu Asp Ile Tyr Pro Tyr Arg Lys Asn Ala Asp Thr Phe Ile Pro 435 440
445Val Tyr Asn Ile Thr Lys Ala Lys Glu Ile Asn Thr Thr Thr Pro Leu
450 455 460Pro Val Asn Tyr Leu Gln Ala Gln Met Ile Asp Ser Asn Asp
Ile Asn465 470 475 480Leu Ser Ser Asp Phe Leu Lys Val Ile Ser Ser
Lys Gly Ser Leu Val 485 490 495Tyr Ser Phe Leu Asn Asn Thr Met Asp
Tyr Leu Glu Phe Ile Lys Tyr 500 505 510Asp Lys Pro Ile Asp Thr Asp
Lys Lys Tyr Tyr Lys Trp Leu Lys Ala 515 520 525Ile Phe Arg Asn Tyr
Ser Leu Asp Ile Thr Glu Thr Gln Glu Ile Ser 530 535 540Asn Gln Phe
Gly Asp Thr Lys Ile Ile Pro Trp Ile Gly Arg Ala Leu545 550 555
560Asn Ile Leu Asn Thr Asn Asn Ser Phe Val Glu Glu Phe Lys Asn Leu
565 570 575Gly Pro Ile Ser Leu Ile Asn Lys Lys Glu Asn Ile Thr Ile
Pro Lys 580 585 590Ile Lys Ile Asp Glu Ile Pro Ser Ser Met Leu Asn
Phe Ser Phe Lys 595 600 605Asp Leu Ser Glu Asn Leu Phe Asn Ile Tyr
Cys Lys Asn Asn Phe Tyr 610 615 620Leu Lys Lys Ile Tyr Tyr Asn Phe
Leu Asp Gln Trp Trp Thr Gln Tyr625 630 635 640Tyr Ser Gln Tyr Phe
Asp Leu Ile Cys Met Ala Ser Lys Ser Val Leu 645 650 655Ala Gln Glu
Lys Leu Ile Lys Lys Leu Ile Gln Lys Gln Leu Arg Tyr 660 665 670Leu
Met Glu Asn Ser Asn Ile Ser Ser Thr Asn Leu Ile Leu Ile Asn 675 680
685Leu Thr Thr Thr Asn Thr Leu Arg Asp Ile Ser Asn Gln Ser Gln Ile
690 695 700Ala Ile Asn Asn Ile Asp Lys Phe Phe Asn Asn Ala Ala Met
Cys Val705 710 715 720Phe Glu Asn Asn Ile Tyr Pro Lys Phe Thr Ser
Phe Met Glu Gln Cys 725 730 735Ile Lys Asn Ile Asn Lys Ser Thr Lys
Glu Phe Ile Leu Lys Cys Thr 740 745 750Asn Ile Asn Glu Thr Glu Lys
Ser His Leu Ile Met Gln Asn Ser Phe 755 760 765Ser Asn Leu Asp Phe
Asp Phe Leu Asp Ile Gln Asn Met Lys Asn Leu 770 775 780Phe Asn Leu
Tyr Thr Glu Leu Leu Ile Lys Glu Gln Thr Ser Pro Tyr785 790 795
800Glu Leu Ser Leu Tyr Ala Phe Gln Glu Gln Asp Asn Asn Val Ile Gly
805 810 815Asp Thr Ser Gly Lys Asn Thr Leu Val Glu Tyr Pro Lys Asp
Ile Gly 820 825 830Leu Val Tyr Gly Ile Asn Asn Asn Ala Ile His Leu
Thr Gly Ala Asn 835 840 845Gln Asn Ile Lys Phe Thr Asn Asp Tyr Phe
Glu Asn Gly Leu Thr Asn 850 855 860Asn Phe Ser Ile Tyr Phe Trp Leu
Arg Asn Leu Lys Gln Asn Thr Ile865 870 875 880Lys Ser Lys Leu Ile
Gly Ser Lys Glu Asp Asn Cys Gly Trp Glu Ile 885 890 895Tyr Phe Glu
Asn Asp Gly Leu Val Phe Asn Ile Ile Asp Ser Asn Gly 900 905 910Asn
Glu Lys Asn Ile Tyr Leu Ser Asn Ile Ser Asn Lys Ser Trp His 915 920
925Tyr Ile Val Ile Ser Ile Asn Arg Leu Lys Asp Gln Leu Leu Ile Phe
930 935 940Ile Asp Asn Ile Leu Val Ala Asn Glu Asp Ile Lys Glu Ile
Leu Asn945 950 955 960Ile Tyr Ser Ser Asp Ile Ile Ser Leu Leu Ser
Asp Asn Asn Asn Val 965 970 975Tyr Ile Glu Gly Leu Ser Val Leu Asn
Lys Thr Ile Asn Ser Asn Glu 980 985 990Ile Leu Thr Asp Tyr Phe Ser
Asp Leu Asn Asn Ser Tyr Ile Arg Asn 995 1000 1005Phe Asp Glu Glu
Ile Leu Gln Tyr Asn Arg Thr Tyr Glu Leu Phe Asn 1010 1015 1020Tyr
Val Phe Pro Glu Ile Ala Ile Asn Lys Ile Glu Gln Asn Asn Asn1025
1030 1035 1040Ile Tyr Leu Ser Ile Asn Asn Glu Asn Asn Leu Asn Phe
Lys Pro Leu 1045 1050 1055Lys Phe Lys Leu Leu Asn Thr Asn Pro Asn
Lys Gln Tyr Val Gln Lys 1060 1065 1070Trp Asp Glu Val Ile Phe Ser
Val Leu Asp Gly Thr Glu Lys Tyr Leu 1075 1080 1085Asp Ile Ser Thr
Thr Asn Asn Arg Ile Gln Leu Val Asp Asn Lys Asn 1090 1095 1100Asn
Ala Gln Ile Phe Ile Ile Asn Asn Asp Ile Phe Ile Ser Asn Cys1105
1110 1115 1120Leu Thr Leu Thr Tyr Asn Asn Val Asn Val Tyr Leu Ser
Ile Lys Asn 1125 1130 1135Gln Asp Tyr Asn Trp Val Ile Cys Asp Leu
Asn His Asp Ile Pro
Lys 1140 1145 1150Lys Ser Tyr Leu Trp Ile Leu Lys Asn Ile 1155
1160301159PRTClostridium botulinum serotype F 30Met Lys Ile Asn Asn
Asn Phe Asn Ile Asp Ser Leu Ile Asp Asn Arg1 5 10 15Asp Val Ala Ile
Val Arg Gly Arg Lys Thr Asp Thr Phe Phe Lys Val 20 25 30Phe Gln Val
Ala Pro Asn Ile Trp Ile Ala Pro Glu Arg Tyr Tyr Gly 35 40 45Glu Ser
Leu Asn Ile Asn Glu Asp Gln Lys Ser Asp Gly Gly Ile Tyr 50 55 60Asp
Ser Asn Phe Leu Ser Thr Asn Asp Glu Lys Asp Glu Phe Leu Gln65 70 75
80Ala Thr Val Lys Ile Leu Gln Arg Ile Asn Asn Asn Val Ile Gly Ala
85 90 95Lys Leu Leu Ser Leu Ile Ser Thr Ala Ile Pro Phe Pro Tyr Glu
Tyr 100 105 110Lys Pro Gly Asp Tyr Arg Gln Thr Asn Tyr Leu Val Ser
Lys Asp Asn 115 120 125Gln His Tyr Tyr Thr Ala Asn Leu Val Ile Phe
Gly Pro Gly Thr Asn 130 135 140Ile Val Glu Asn Asn Ala Ile Tyr Tyr
Lys Lys Glu Asp Ser Glu Asn145 150 155 160Gly Met Gly Thr Met Ser
Glu Ile Trp Phe Gln Pro Phe Leu Thr Tyr 165 170 175Lys Tyr Gly Gln
Phe Tyr Val Asp Pro Ala Leu Glu Leu Ile Lys Cys 180 185 190Leu Ile
Lys Ser Leu Tyr Tyr Leu Tyr Gly Ile Lys Pro Ser Asp Asp 195 200
205Leu Ser Ile Pro Tyr Arg Leu Arg Ser Glu Leu Asn Ser Phe Glu Tyr
210 215 220Ser Glu Leu Asp Met Ile Asp Phe Leu Ile Ser Gly Gly Thr
Glu Tyr225 230 235 240Lys Leu Leu Asp Thr Asn Pro Tyr Trp Phe Thr
Asp Asn Tyr Phe Ile 245 250 255Asp Ala Pro Lys Asn Phe Glu Lys Tyr
Lys Asn Asp Tyr Glu Thr Lys 260 265 270Ile Lys Asn Asn Asn Asp Ile
Ala Asn Ser Ile Lys Leu Tyr Leu Glu 275 280 285Gln Lys Phe Lys Thr
Asn Ala Gln Asp Ile Trp Glu Leu Asn Leu Ser 290 295 300Tyr Phe Ser
Thr Glu Phe Glu Ile Met Met Pro Glu Ile Phe Asn Asn305 310 315
320Ala Leu Asn His Tyr Tyr Arg Lys Glu Tyr Tyr Val Ile Asp Tyr Phe
325 330 335Lys Asn Tyr Asn Ile Asn Gly Phe Ile Asn Gly Gln Ile Lys
Thr Ile 340 345 350Leu Pro Leu Ser Lys Tyr Asn Lys Asn Ile Ile Asn
Lys Pro Glu Leu 355 360 365Val Val Asn Leu Ile Asn Glu Asn Asn Thr
Val Leu Met Lys Ser Asn 370 375 380Val Tyr Gly Asp Gly Leu Lys Gly
Thr Met Asp Asn Phe Tyr Ala Ala385 390 395 400Tyr Lys Ile Pro Tyr
Asn Ile Gly Asp Glu Tyr His Ile Asn Tyr Ser 405 410 415Tyr Leu Asn
Asn Val Asn Val Glu Glu Ile Asn Asn Ile Pro Pro Ile 420 425 430Asn
Asp Ala Asp Ile Tyr Pro Tyr Arg Lys Asn Ser Asp Pro Phe Ile 435 440
445Pro Val Tyr Asn Ile Thr Glu Thr Lys Glu Ile Asn Thr Thr Thr Pro
450 455 460Leu Ser Val Asn Tyr Leu Gln Ala Gln Val Thr Asn Ser Asn
Asp Ile465 470 475 480Ser Leu Ser Ser Asp Phe Ser Lys Val Ile Ser
Ser Lys Asp Arg Ser 485 490 495Leu Val Tyr Ser Phe Leu Asp Asn Thr
Ile Asp Tyr Leu Asp Ser Ile 500 505 510Lys Tyr Asp Glu Pro Ile Asp
Thr Asp Lys Lys Tyr Tyr Leu Trp Leu 515 520 525Lys Glu Ile Phe Arg
Asn Tyr Ser Phe Asp Met Thr Glu Thr Gln Glu 530 535 540Val Asn Thr
Pro Cys Gly Ile Asn Lys Val Val Pro Trp Leu Gly Lys545 550 555
560Ala Leu Asn Ile Leu Asn Thr Gly Asn Ser Phe Ile Glu Glu Phe Lys
565 570 575Ser Leu Gly Pro Ile Ser Leu Ile Asn Lys Lys Glu Asn Ile
Thr Met 580 585 590Pro Lys Ile Glu Ile Asp Glu Ile Pro Asn Ser Met
Leu Asn Leu Ser 595 600 605Phe Lys Asp Leu Ser Glu Asn Leu Phe Asn
Arg Phe Ser Lys Asn Asn 610 615 620Ser Tyr Phe Glu Lys Ile Tyr Tyr
Asp Phe Leu Asp Gln Trp Trp Thr625 630 635 640Gln Tyr Tyr Ser Gln
Tyr Phe Asp Leu Ile Cys Met Ala Lys Lys Ser 645 650 655Ile Leu Ala
Gln Glu Thr Leu Ile Lys Lys Ile Ile Gln Lys Lys Leu 660 665 670Ser
Tyr Leu Ile Gly Asn Ser Asn Ile Ser Ser Asp Asn Leu Ala Leu 675 680
685Met Asn Leu Thr Thr Thr Asn Thr Leu Arg Asp Ile Ser Asn Glu Ser
690 695 700Gln Ile Ala Met Asn Asn Val Asp Ser Phe Leu Asn Ser Ala
Ala Ile705 710 715 720Cys Val Phe Glu Gly Asn Ile Tyr Ser Lys Phe
Ile Ser Phe Met Glu 725 730 735Gln Cys Ile Asn Asn Ile Asn Lys Asn
Thr Arg Glu Phe Ile Gln Lys 740 745 750Cys Thr Asn Ile Thr Glu Asn
Glu Lys Leu Gln Leu Ile Asn Gln Asn 755 760 765Ile Phe Ser Ser Leu
Asp Phe Asp Phe Leu Asn Ile Glu Asn Leu Lys 770 775 780Ser Leu Phe
Ser Ser Glu Thr Ala Leu Leu Ile Lys Glu Glu Thr Ser785 790 795
800Pro Tyr Glu Leu Val Leu Tyr Ala Phe Gln Glu Pro Asp Asn Asn Ala
805 810 815Ile Gly Asp Ala Ser Ala Lys Asn Thr Ser Ile Glu Tyr Ser
Lys Asp 820 825 830Ile Asp Leu Val Tyr Gly Ile Asn Ser Asp Ala Leu
Tyr Leu Asn Gly 835 840 845Ser Asn Gln Ser Ile Ser Phe Ser Asn Asp
Phe Phe Glu Asn Gly Leu 850 855 860Thr Asn Ser Phe Ser Ile Tyr Phe
Trp Leu Arg Asn Leu Gly Lys Asp865 870 875 880Thr Ile Lys Tyr Lys
Leu Ile Gly Ser Lys Glu Asp Asn Cys Gly Trp 885 890 895Glu Ile Tyr
Phe Gln Asp Thr Gly Leu Val Phe Asn Met Ile Asp Ser 900 905 910Asn
Gly Asn Glu Lys Asn Ile Tyr Leu Ser Asp Val Ser Asn Asn Ser 915 920
925Trp His Tyr Ile Thr Ile Ser Val Asp Arg Leu Lys Glu Gln Leu Leu
930 935 940Ile Phe Ile Asp Asp Asn Leu Val Ala Asn Glu Ser Ile Lys
Glu Ile945 950 955 960Leu Asn Ile Tyr Ser Ser Asn Ile Ile Ser Leu
Leu Ser Glu Asn Lys 965 970 975Pro Ser Tyr Ile Glu Gly Leu Thr Ile
Leu Asn Lys Pro Thr Thr Ser 980 985 990Gln Glu Val Leu Asn Asn Tyr
Phe Lys Val Leu Asn Asn Ser Tyr Ile 995 1000 1005Arg Asp Ser Asn
Glu Glu Arg Leu Glu Tyr His Lys Thr Tyr Gln Leu 1010 1015 1020Asp
Asn Tyr Val Phe Ser Asp Lys Pro Ile Cys Glu Val Lys Gln Asn1025
1030 1035 1040Asn Asn Ile Tyr Leu Thr Ile Asn Asn Thr Asn Asn Leu
Asn Leu Gln 1045 1050 1055Pro Ser Lys Phe Lys Leu Leu Ser Ile Asn
Ser Asn Lys Gln Tyr Val 1060 1065 1070Gln Lys Phe Asp Glu Val Ile
Ile Ser Ile Leu Gly Asn Met Glu Lys 1075 1080 1085Tyr Ile Asp Ile
Ser Glu Asp Asn Arg Leu Gln Leu Ile Asp Asn Lys 1090 1095 1100Asn
Gly Ala Lys Lys Met Ile Ile Ser Asn Asp Met Phe Ile Ser Asn1105
1110 1115 1120Cys Leu Thr Leu Ser Cys Gly Gly Lys Tyr Ile Cys Leu
Ser Met Lys 1125 1130 1135Asp Glu Asn His Asn Trp Met Ile Cys Asn
Asn Asp Met Ser Lys Tyr 1140 1145 1150Leu Tyr Leu Trp Ser Phe Lys
1155311165PRTClostridium botulinum serotype F 31Met Lys Ile Asn Asp
Asp Leu Asn Ile Asn Ser Pro Val Asp Asn Lys1 5 10 15Asn Val Val Ile
Val Arg Ala Arg Lys Thr Asn Ile Phe Phe Lys Ala 20 25 30Phe Gln Val
Ala Pro Asn Ile Trp Val Ala Pro Glu Arg Tyr Tyr Gly 35 40 45Glu Pro
Leu Asn Ile Ser Asp Gln Glu Lys Ser Asp Gly Gly Ile Tyr 50 55 60Asp
Glu Asn Phe Leu Lys Glu Asn Ser Glu Lys Glu Glu Phe Leu Gln65 70 75
80Ala Ile Ile Leu Leu Leu Lys Arg Ile Asn Asn Asn Ile Ile Gly Gln
85 90 95Lys Leu Leu Ser Leu Met Cys Thr Ser Ile Pro Phe Leu His Glu
Tyr 100 105 110Lys Gln Gly Asp Tyr Arg Gln Ser Asn Tyr Leu Gly Ser
Lys Asn Ser 115 120 125Glu Tyr Leu Tyr Ser Ala Asn Ile Val Ile Phe
Gly Pro Gly Ser Asn 130 135 140Ile Val Lys Asn Asn Thr Ile Tyr Tyr
Lys Lys Asn Phe Ala Glu Asn145 150 155 160Gly Met Gly Thr Met Ala
Glu Ile Leu Phe Gln Pro Phe Leu Thr Tyr 165 170 175Lys Tyr Asn Gln
Phe Tyr Ala Asp Pro Ala Leu Glu Leu Ile Lys Cys 180 185 190Leu Ile
Lys Ala Ile Tyr Phe Leu Tyr Gly Ile Lys Pro Asn Asp Asn 195 200
205Leu Asn Ile Pro Tyr Arg Leu Arg Asn Glu Phe Ser Asn Val Glu Tyr
210 215 220Ser Glu Leu Asn Ile Ile Asp Phe Leu Ile Ser Gly Gly Ile
Asp Tyr225 230 235 240Lys Phe Ile Asn Thr Asn Pro Tyr Trp Phe Ile
Asp Asn Tyr Phe Ile 245 250 255Asp Val Pro Lys Val Phe Glu Lys His
Lys Asn Asp Tyr Glu Ile Asn 260 265 270Ile Lys Asn Asn Ser Glu Ile
Gly Thr Ser Ile Lys Leu Tyr Leu Glu 275 280 285Gln Lys Phe Lys Thr
Asn Val Gln Asp Ile Trp Glu Leu Asn Leu Ser 290 295 300Tyr Phe Ser
Lys Glu Phe Gln Ile Met Met Pro Glu Lys His Asn Asn305 310 315
320Ala Leu Lys His Tyr Tyr Arg Lys Glu Tyr Tyr Lys Ile Asn Tyr Ser
325 330 335Lys Gln Tyr Asp Ile Asn Gly Phe Val Asn Gly Gln Ile Ala
Thr Lys 340 345 350Leu Leu Leu Ser Glu Lys Asn Gln Tyr Ile Ile Asn
Lys Pro Gln Leu 355 360 365Ile Ile Asn Leu Ile Asn Lys Ser Asn Asn
Ser Leu Leu Met Lys Ser 370 375 380Asn Ile Tyr Gly Asp Gly Leu Asn
Gly Thr Thr Asp Asn Phe Tyr Arg385 390 395 400Asn Tyr Lys Ile Pro
Asp Asn Ile Ala Tyr Gln Tyr His Pro Asn Asn 405 410 415Thr Tyr Leu
Asp Asn Val Asn Ile Glu Glu Ile Asn Asn Ile Pro Gln 420 425 430Ile
Thr Asp Ala Asp Ile Tyr Pro Tyr Thr Asn Asn Cys Asp Thr Phe 435 440
445Ile Pro Ile Tyr Asn Ile Thr Gln Ser Arg Glu Ile Asn Thr Thr Val
450 455 460Pro Tyr Ser Ile Asn Tyr Leu Gln Ser Gln Ile Met Asn Ser
Asp Asp465 470 475 480Ile Thr Leu Ser Ser Asp Phe Trp Glu Val Val
Cys Ser Asn Asp Lys 485 490 495Ser Leu Val Tyr Ser Tyr Leu Asp Asn
Val Ile Asn Tyr Leu Asp Ser 500 505 510Ile Lys Asn Asn Thr Pro Ile
Asn Thr Asp Lys Lys Tyr Tyr Leu Trp 515 520 525Leu Lys Glu Ile Phe
Arg Asn Tyr Ser Phe Asp Ile Thr Ala Thr Glu 530 535 540Glu Ile Thr
Thr Glu Cys Gly Ile Asn Lys Ile Val Ser Trp Phe Gly545 550 555
560Lys Ala Phe Asn Ile Leu Asn Thr Asp Asn Ser Phe Lys Ile Glu Phe
565 570 575Gln Asn Ser Gly Ala Ile Ala Leu Ile Asn Lys Lys Asp Asn
Ile Ile 580 585 590Ile Pro Lys Ile Glu Ile Asp Glu Met Pro Asn Ser
Met Leu Asn Leu 595 600 605Ser Phe Glu Asp Leu Asn Glu Gln Leu Tyr
Ser Ile Tyr Ser Lys Asn 610 615 620Ile Thr Tyr Phe Lys Lys Ile Tyr
Tyr Asn Phe Leu Asp Gln Trp Trp625 630 635 640Thr Glu Tyr Tyr Ser
Gln Tyr Phe Asp Leu Ile Cys Met Ala Lys Lys 645 650 655Ser Ile Leu
Ala Gln Glu Asn Leu Ile Lys Lys Ile Ile Gln Lys Lys 660 665 670Ile
Ser Tyr Leu Ile Gly Ala Ser Asn Ile Pro Asp Asp Ile Leu Ala 675 680
685Val Met Arg Leu Thr Thr Thr Asn Thr Leu Arg Asp Ile Ser Val Glu
690 695 700Ser Gln Ile Ala Met Asn Asn Leu Asn Asn Phe Leu Asn Lys
Ala Ala705 710 715 720Met Cys Val Phe Gln Ser Asn Ile Tyr Pro Lys
Phe Ile Ser Phe Met 725 730 735Glu Gln Cys Ile Lys His Ile Asn Lys
Ser Thr Lys Glu Phe Ile Gln 740 745 750Lys Cys Thr Asn Ile Asn Glu
Thr Glu Lys Leu Gln Leu Ile Met Gln 755 760 765Asn Ser Phe Ser Asn
Leu Asp Phe Asp Phe Leu Asp Ile Gln Asn Met 770 775 780Lys Asn Leu
Phe Asn Ser Tyr Thr Glu Leu Leu Ile Lys Glu Gln Thr785 790 795
800Ser Pro Tyr Glu Leu Ser Leu Tyr Ala Phe Glu Glu Gln Asp Asn Asn
805 810 815Val Ile Gly Asp Ala Ser Gly Lys Asn Thr Leu Val Glu Tyr
Pro Lys 820 825 830Gly Ile Glu Leu Val Tyr Gly Ile Asn Asn Ser Ala
Leu Tyr Leu Asn 835 840 845Gly Ser Asn Gln Ser Ile Ile Phe Thr Asn
Asp Tyr Phe Glu Asn Gly 850 855 860Leu Thr Asn Ser Phe Ser Ile Tyr
Phe Trp Leu Arg Asn Leu Gly Gln865 870 875 880Asp Thr Ile Lys Ser
Lys Leu Ile Gly Ser Lys Glu Tyr Asn Cys Gly 885 890 895Trp Glu Ile
Tyr Phe Gln Glu Ile Gly His Val Phe Asn Met Ile Asp 900 905 910Ser
Asn Gly Asn Glu Lys Asn Ile Tyr Leu Ser Asp Val Ser Asn Asn 915 920
925Ser Trp His Tyr Ile Thr Ile Ser Val Asp Arg Leu Lys Glu Gln Leu
930 935 940Leu Ile Phe Ile Asp Asp Asn Leu Val Val Asn Glu Ser Ile
Lys Asp945 950 955 960Ile Leu Asn Ile Tyr Ser Ser Asn Ile Ile Ser
Leu Leu Ser Asp Asn 965 970 975Lys Ala Ser Tyr Ile Glu Gly Leu Thr
Ile Leu Asn Lys Pro Thr Thr 980 985 990Gly Glu Glu Val Leu Arg Asn
Tyr Phe Lys Asn Leu Asn Asn Ser Tyr 995 1000 1005Val Arg Asp Ser
Asn Asp Glu Arg Leu Glu Tyr Asn Lys Thr Tyr Gln 1010 1015 1020Leu
Tyr Asp Tyr Val Phe Pro Asp Asn Pro Ile Cys Glu Val Lys Gln1025
1030 1035 1040Asp Asn Asn Ile Tyr Leu Thr Ile Asn Asn Ile Asn Asn
Leu Asn Met 1045 1050 1055Lys Pro Cys Lys Phe Lys Leu Leu Ser Ile
Asn Ser Asn Lys Gln Tyr 1060 1065 1070Val Gln Lys Trp Asp Glu Val
Ile Ile Ser Val Leu Tyr Asp Thr Glu 1075 1080 1085Lys Tyr Val Cys
Ile Ser Asn Glu Asn Asn Arg Val Lys Ile Ile Asp 1090 1095 1100Asn
Lys Ile Met Gln Val Lys Phe Ile Ile Ser Asn Asp Ile Phe Ile1105
1110 1115 1120Ser Asn Cys Leu Thr His Ala His Asn Asn Lys Tyr Ile
Cys Leu Ser 1125 1130 1135Met Lys Asp Glu Asn Tyr Asn Trp Met Ile
Cys Asn Asn Glu Ser Asn 1140 1145 1150Ile Pro Lys Lys Ala Tyr Leu
Trp Ile Leu Lys Glu Val 1155 1160 1165321198PRTClostridium
botulinum serotype G 32Met Lys Ile Asn Ser Asn Leu Thr Ile Asn Ser
Pro Ile Asp Asn Lys1 5 10 15Asn Val Val Ile Val Arg Ala Arg Glu Thr
Ser Lys Phe Phe Lys Ala 20 25 30Phe Lys Val Ala Pro Asn Ile Trp Val
Ala Pro Glu Arg Tyr Tyr Gly 35 40 45Glu Ser Leu Ser Ile Glu Glu Ser
Lys Lys Val Asn Gly Gly Val Tyr 50 55 60Asp Ser Asn Phe Leu Ser Gln
Asn Asn Glu Lys Asp Lys Phe Leu Gln65 70 75 80Ala Ile Ile Thr Leu
Leu Lys Arg Ile Asn Ser Asn Ile Ala Gly Glu 85 90 95Lys Leu Leu Ser
Leu Val Ser Thr Ala Ile Pro Phe Pro Tyr Gly Tyr 100
105 110Ile Gly Gly Gly Tyr Tyr Cys Pro Asn Ile Val Thr Phe Gly Ser
Thr 115 120 125Ile Lys Tyr Asn Lys Lys Ile Asn Ser Leu Ile Ser Thr
Thr Ile Pro 130 135 140Phe Pro Tyr Gly Gly Tyr Arg Glu Thr Asn Tyr
Leu Ser Ser Lys Asp145 150 155 160Thr Glu Asn Phe Tyr Ala Ala Asn
Ile Val Ile Phe Gly Pro Gly Ala 165 170 175Asn Ile Val Glu Asn Asn
Thr Val Phe Tyr Lys Lys Glu Asp Ala Glu 180 185 190Asn Gly Met Gly
Thr Met Ala Glu Ile Cys Phe Gln Pro Phe Leu Thr 195 200 205Tyr Lys
Tyr Asp Gln Phe Tyr Val Asp Pro Ala Leu Glu Leu Met Glu 210 215
220Cys Leu Ile Lys Ser Leu Tyr Phe Leu Tyr Gly Ile Lys Pro Asn
Asn225 230 235 240Asn Leu Thr Val Pro Tyr Arg Leu Arg Asn Glu Leu
Ser Asn Ile Glu 245 250 255Phe Ser Gln Leu Ser Ile Val Asp Leu Leu
Ile Ser Gly Gly Ile Asp 260 265 270Ser Lys Phe Ile Asn Thr Asp Pro
Tyr Trp Phe Ile Asp Ser Tyr Phe 275 280 285Ser Asn Ala Lys Thr Thr
Phe Glu Glu His Lys Ser Ile Tyr Glu Thr 290 295 300Glu Ile Lys Gly
Asn Asn Ala Ile Gly Asn Asp Ile Lys Leu Arg Leu305 310 315 320Lys
Gln Lys Phe Gln Thr Thr Val His Asp Ile Trp Gln Leu Asn Leu 325 330
335Asp Tyr Phe Ser Lys Glu Phe Gln Ile Met Met Pro Tyr Arg Phe Asn
340 345 350Asn Ala Leu Lys Tyr Tyr Tyr Arg Lys Glu Tyr Tyr Lys Ile
Asp Tyr 355 360 365Pro Glu Lys Tyr Ser Ile Ala Gly Phe Val Asp Gly
Gln Leu Asn Thr 370 375 380Gln Leu Ser Leu Ser Asp Lys Asn Gln Tyr
Ile Ile Asn Lys Pro Glu385 390 395 400Leu Ile Val Asn Leu Ile Ser
Glu Asn Asn Ile Ser Leu Met Arg Ser 405 410 415Asn Ile Tyr Gly Asp
Gly Leu Lys Tyr Thr Thr Asp Asn Phe Tyr Ser 420 425 430Thr Tyr Lys
Ile Pro Tyr Asn Arg Ala Tyr Glu Tyr His Phe Asn Asn 435 440 445Ser
Ser Thr Ser Ser Leu Glu Asn Val Asn Val Glu Glu Ile Ser Asn 450 455
460Ile Pro Glu Ile Ile Asp Ile Asn Pro Tyr Arg Glu Asn Ser Asp
Ile465 470 475 480Phe Ser Pro Val Glu Asn Ile Ile Glu Thr Lys Glu
Val Asn Thr Lys 485 490 495Thr Pro Trp Pro Ile Asn Tyr Leu Gln Ala
Gln Ile Pro Asn Asn Glu 500 505 510Glu Phe Thr Leu Ser Ser Asp Phe
Ser Gln Val Val Ser Tyr Lys Thr 515 520 525Gln Ser Leu Val Tyr Ser
Phe Leu Ser Asn Val Ile Ser Tyr Leu Asp 530 535 540Ser Val Lys Asp
Thr Asn Pro Ile Asp Thr Asp Glu Lys Tyr Tyr Leu545 550 555 560Trp
Leu Arg Glu Ile Phe Arg Asn Tyr Ser Phe Asp Ile Thr Ala Ile 565 570
575Glu Glu Ile Asn Thr Ser Cys Gly Ile Asn Lys Val Val Ser Trp Phe
580 585 590Gly Lys Ala Leu Asn Ile Leu Asn Thr Ser Asn Ser Phe Val
Lys Glu 595 600 605Phe Lys Asn Leu Gly Pro Ile Ser Leu Ile Asn Lys
Lys Glu Asn Leu 610 615 620Ser Met Pro Ile Ile Glu Val Asn Glu Ile
Pro Asn Asp Met Leu Gly625 630 635 640Leu Ser Leu Lys Asp Leu Asn
Glu Lys Leu Phe Asn Ile Tyr Leu Lys 645 650 655Asn Ile Leu Tyr Phe
Lys Lys Val Tyr Phe Ser Phe Leu Asp Gln Trp 660 665 670Trp Thr Glu
Tyr Tyr Ser Gln Tyr Phe Gly Leu Ile Cys Met Ala Lys 675 680 685Gln
Ser Ile Leu Ala Gln Glu Asn Leu Ile Lys Lys Ile Val Gln Lys 690 695
700Lys Leu Ser Asp Leu Ser Lys Gln Ser Asn Ile Ser Asn Glu Lys
Leu705 710 715 720Asn Leu Met Asn Leu Thr Thr Glu Lys Thr Phe Ile
Asp Leu Ser Asn 725 730 735Gln Ser Gln Ile Ala Met Asn Asn Ile Asn
Asn Phe Leu Asn Lys Ala 740 745 750Ala Ile Cys Val Phe Glu Ser Asn
Ile Tyr Pro Lys Phe Ile Ser Phe 755 760 765Met Glu Gln Tyr Ile Asn
Asn Ile Asn Ile Lys Thr Thr Ala Phe Ile 770 775 780Arg Lys Cys Thr
Asn Ile Thr Glu Lys Glu Lys Leu Gln Leu Ile Asn785 790 795 800Gln
Asn Thr Phe Asn Asn Leu Asp Phe Glu Phe Phe Asp Ile Gln Thr 805 810
815Ile Glu Asn Leu Leu Thr Ser Glu Thr Asn Leu Ile Ile Lys Glu Lys
820 825 830Thr Ser Pro Tyr Asp Leu Leu Leu Phe Ser Leu Gln Glu Ala
Asp Arg 835 840 845Lys Val Ile Lys Asp Ile Ser Gly Lys Asp Thr Leu
Val Gln Tyr Ser 850 855 860Asp Thr Ile Asp Leu Ser Tyr Gly Val Asn
Gly Asp Ala Leu Tyr Leu865 870 875 880Lys Glu Pro Asn Gln Ser Val
Asn Phe Ser Asn Asn Ile Phe Glu Asn 885 890 895Gly Leu Thr Asn Ser
Phe Ser Ile Cys Phe Trp Leu Arg Asn Leu Gly 900 905 910Gln Asp Asn
Leu Ser Ser Asn Leu Ile Gly Asn Ile Val Asn Asn Cys 915 920 925Gly
Trp Gln Ile Tyr Phe Glu Asn Asn Gly Leu Val Phe Ser Met Val 930 935
940Asp Cys Asn Gly Asn Glu Lys Asn Ile Tyr Leu Ser Asp Val Leu
Ser945 950 955 960Lys Tyr Trp Tyr Tyr Ile Ser Val Ser Val Asp Arg
Leu Arg Asn Lys 965 970 975Leu Leu Ile Phe Ile Asn Asp Lys Leu Ile
Val Asn Glu Ser Ile Glu 980 985 990Gln Ile Leu Asn Ile Tyr Ser Ser
Asn Ile Ile Ser Leu Val Asn Glu 995 1000 1005Asn Asn Pro Ile Cys
Ile Glu Glu Leu Ser Ile Leu Asn Lys Ala Leu 1010 1015 1020Thr Ser
Glu Glu Val Leu Asn Ser Tyr Phe Thr Asn Leu Asn Asn Ser1025 1030
1035 1040Tyr Ile Arg Asp Ser Tyr Gly Ala Arg Leu Glu Tyr Asn Lys
Asn Tyr 1045 1050 1055Glu Leu Tyr Asn Tyr Val Phe Pro Glu Asn Ser
Leu Tyr Glu Val Ile 1060 1065 1070Glu Asn Asn Asn Met Tyr Leu Ser
Ile Lys Asn Ile Lys Asn Thr Asn 1075 1080 1085Ile Leu Gly Ala Lys
Phe Lys Leu Ile Asn Thr Asp Glu Ser Lys Gln 1090 1095 1100Tyr Val
Gln Lys Trp Asp Glu Val Ile Ile Cys Val Leu Gly Asp Thr1105 1110
1115 1120Glu Lys Tyr Ala Asp Ile Gln Ala Gly Asn Asn Arg Ile Gln
Leu Val 1125 1130 1135Asn Ser Lys Asp Asn Ala Arg Lys Ile Ile Val
Asn Asn Asn Ile Phe 1140 1145 1150Arg Pro Asn Cys Val Leu Phe Ser
Tyr Asn Asn Lys Tyr Leu Ser Leu 1155 1160 1165Ser Leu Arg Asn Arg
Asn Tyr Asn Trp Met Ile Cys Asn Asp Asn Ser 1170 1175 1180Phe Ile
Pro Lys His Ala His Leu Trp Ile Leu Lys Lys Ile1185 1190
119533155PRTHomo sapiens 33Met Ala Glu Gly Glu Ile Thr Thr Phe Thr
Ala Leu Thr Glu Lys Phe1 5 10 15Asn Leu Pro Pro Gly Asn Tyr Lys Lys
Pro Lys Leu Leu Tyr Cys Ser 20 25 30Asn Gly Gly His Phe Leu Arg Ile
Leu Pro Asp Gly Thr Val Asp Gly 35 40 45Thr Arg Asp Arg Ser Asp Gln
His Ile Gln Leu Gln Leu Ser Ala Glu 50 55 60Ser Val Gly Glu Val Tyr
Ile Lys Ser Thr Glu Thr Gly Gln Tyr Leu65 70 75 80Ala Met Asp Thr
Asp Gly Leu Leu Tyr Gly Ser Gln Thr Pro Asn Glu 85 90 95Glu Cys Leu
Phe Leu Glu Arg Leu Glu Glu Asn His Tyr Asn Thr Tyr 100 105 110Ile
Ser Lys Lys His Ala Glu Lys Asn Trp Phe Val Gly Leu Lys Lys 115 120
125Asn Gly Ser Cys Lys Arg Gly Pro Arg Thr His Tyr Gly Gln Lys Ala
130 135 140Ile Leu Phe Leu Pro Leu Pro Val Ser Ser Asp145 150
15534155PRTHomo sapiens 34Met Ala Ala Gly Ser Ile Thr Thr Leu Pro
Ala Leu Pro Glu Asp Gly1 5 10 15Gly Ser Gly Ala Phe Pro Pro Gly His
Phe Lys Asp Pro Lys Arg Leu 20 25 30Tyr Cys Lys Asn Gly Gly Phe Phe
Leu Arg Ile His Pro Asp Gly Arg 35 40 45Val Asp Gly Val Arg Glu Lys
Ser Asp Pro His Ile Lys Leu Gln Leu 50 55 60Gln Ala Glu Glu Arg Gly
Val Val Ser Ile Lys Gly Val Cys Ala Asn65 70 75 80Arg Tyr Leu Ala
Met Lys Glu Asp Gly Arg Leu Leu Ala Ser Lys Cys 85 90 95Val Thr Asp
Glu Cys Phe Phe Phe Glu Arg Leu Glu Ser Asn Asn Tyr 100 105 110Asn
Thr Tyr Arg Ser Arg Lys Tyr Thr Ser Trp Tyr Val Ala Leu Lys 115 120
125Arg Thr Gly Gln Tyr Lys Leu Gly Ser Lys Thr Gly Pro Gly Gln Lys
130 135 140Ala Ile Leu Phe Leu Pro Met Ser Ala Lys Ser145 150
15535206PRTHomo sapiens 35Met Ser Gly Pro Gly Thr Ala Ala Val Ala
Leu Leu Pro Ala Val Leu1 5 10 15Leu Ala Leu Leu Ala Pro Trp Ala Gly
Arg Gly Gly Ala Ala Ala Pro 20 25 30Thr Ala Pro Asn Gly Thr Leu Glu
Ala Glu Leu Glu Arg Arg Trp Glu 35 40 45Ser Leu Val Ala Leu Ser Leu
Ala Arg Leu Pro Val Ala Ala Gln Pro 50 55 60Lys Glu Ala Ala Val Gln
Ser Gly Ala Gly Asp Tyr Leu Leu Gly Ile65 70 75 80Lys Arg Leu Arg
Arg Leu Tyr Cys Asn Val Gly Ile Gly Phe His Leu 85 90 95Gln Ala Leu
Pro Asp Gly Arg Ile Gly Gly Ala His Ala Asp Thr Arg 100 105 110Asp
Ser Leu Leu Glu Leu Ser Pro Val Glu Arg Gly Val Val Ser Ile 115 120
125Phe Gly Val Ala Ser Arg Phe Phe Val Ala Met Ser Ser Lys Gly Lys
130 135 140Leu Tyr Gly Ser Pro Phe Phe Thr Asp Glu Cys Thr Phe Lys
Glu Ile145 150 155 160Leu Leu Pro Asn Asn Tyr Asn Ala Tyr Glu Ser
Tyr Lys Tyr Pro Gly 165 170 175Met Phe Ile Ala Leu Ser Lys Asn Gly
Lys Thr Lys Lys Gly Asn Arg 180 185 190Val Ser Pro Thr Met Lys Val
Thr His Phe Leu Pro Arg Leu 195 200 20536204PRTHomo sapiens 36Met
Gly Ser Pro Arg Ser Ala Leu Ser Cys Leu Leu Leu His Leu Leu1 5 10
15Val Leu Cys Leu Gln Ala Gln His Val Arg Glu Gln Ser Leu Val Thr
20 25 30Asp Gln Leu Ser Arg Arg Leu Ile Arg Thr Tyr Gln Leu Tyr Ser
Arg 35 40 45Thr Ser Gly Lys His Val Gln Val Leu Ala Asn Lys Arg Ile
Asn Ala 50 55 60Met Ala Glu Asp Gly Asp Pro Phe Ala Lys Leu Ile Val
Glu Thr Asp65 70 75 80Thr Phe Gly Ser Arg Val Arg Val Arg Gly Ala
Glu Thr Gly Leu Tyr 85 90 95Ile Cys Met Asn Lys Lys Gly Lys Leu Ile
Ala Lys Ser Asn Gly Lys 100 105 110Gly Lys Asp Cys Val Phe Thr Glu
Ile Val Leu Glu Asn Asn Tyr Thr 115 120 125Ala Leu Gln Asn Ala Lys
Tyr Glu Gly Trp Tyr Met Ala Phe Thr Arg 130 135 140Lys Gly Arg Pro
Arg Lys Gly Ser Lys Thr Arg Gln His Gln Arg Glu145 150 155 160Val
His Phe Met Lys Arg Leu Pro Arg Gly His His Thr Thr Glu Gln 165 170
175Ser Leu Arg Phe Glu Phe Leu Asn Tyr Pro Pro Phe Thr Arg Ser Leu
180 185 190Arg Gly Ser Gln Arg Thr Trp Ala Pro Glu Pro Arg 195
20037208PRTHomo sapiens 37Met Ala Pro Leu Gly Glu Val Gly Asn Tyr
Phe Gly Val Gln Asp Ala1 5 10 15Val Pro Phe Gly Asn Val Pro Val Leu
Pro Val Asp Ser Pro Val Leu 20 25 30Leu Ser Asp His Leu Gly Gln Ser
Glu Ala Gly Gly Leu Pro Arg Gly 35 40 45Pro Ala Val Thr Asp Leu Asp
His Leu Lys Gly Ile Leu Arg Arg Arg 50 55 60Gln Leu Tyr Cys Arg Thr
Gly Phe His Leu Glu Ile Phe Pro Asn Gly65 70 75 80Thr Ile Gln Gly
Thr Arg Lys Asp His Ser Arg Phe Gly Ile Leu Glu 85 90 95Phe Ile Ser
Ile Ala Val Gly Leu Val Ser Ile Arg Gly Val Asp Ser 100 105 110Gly
Leu Tyr Leu Gly Met Asn Glu Lys Gly Glu Leu Tyr Gly Ser Glu 115 120
125Lys Leu Thr Gln Glu Cys Val Phe Arg Glu Gln Phe Glu Glu Asn Trp
130 135 140Tyr Asn Thr Tyr Ser Ser Asn Leu Tyr Lys His Val Asp Thr
Gly Arg145 150 155 160Arg Tyr Tyr Val Ala Leu Asn Lys Asp Gly Thr
Pro Arg Glu Gly Thr 165 170 175Arg Thr Lys Arg His Gln Lys Phe Thr
His Phe Leu Pro Arg Pro Val 180 185 190Asp Pro Asp Lys Val Pro Glu
Leu Tyr Lys Asp Ile Leu Ser Gln Ser 195 200 20538216PRTHomo sapiens
38Met Gly Ala Ala Arg Leu Leu Pro Asn Leu Thr Leu Cys Leu Gln Leu1
5 10 15Leu Ile Leu Cys Cys Gln Thr Gln Gly Glu Asn His Pro Ser Pro
Asn 20 25 30Phe Asn Gln Tyr Val Arg Asp Gln Gly Ala Met Thr Asp Gln
Leu Ser 35 40 45Arg Arg Gln Ile Arg Glu Tyr Gln Leu Tyr Ser Arg Thr
Ser Gly Lys 50 55 60His Val Gln Val Thr Gly Arg Arg Ile Ser Ala Thr
Ala Glu Asp Gly65 70 75 80Asn Lys Phe Ala Lys Leu Ile Val Glu Thr
Asp Thr Phe Gly Ser Arg 85 90 95Val Arg Ile Lys Gly Ala Glu Ser Glu
Lys Tyr Ile Cys Met Asn Lys 100 105 110Arg Gly Lys Leu Ile Gly Lys
Pro Ser Gly Lys Ser Lys Asp Cys Val 115 120 125Phe Thr Glu Ile Val
Leu Glu Asn Asn Tyr Thr Ala Phe Gln Asn Ala 130 135 140Arg His Glu
Gly Trp Phe Met Ala Phe Thr Arg Gln Gly Arg Pro Arg145 150 155
160Gln Ala Ser Arg Ser Arg Gln Asn Gln Arg Glu Ala His Phe Ile Lys
165 170 175Arg Leu Tyr Gln Gly Gln Leu Pro Phe Pro Asn His Ala Glu
Lys Gln 180 185 190Lys Gln Phe Glu Phe Val Gly Ser Ala Pro Thr Arg
Arg Thr Lys Arg 195 200 205Thr Arg Arg Pro Gln Pro Leu Thr 210
21539207PRTHomo sapiens 39Met Tyr Ser Ala Pro Ser Ala Cys Thr Cys
Leu Cys Leu His Phe Leu1 5 10 15Leu Leu Cys Phe Gln Val Gln Val Leu
Val Ala Glu Glu Asn Val Asp 20 25 30Phe Arg Ile His Val Glu Asn Gln
Thr Arg Ala Arg Asp Asp Val Ser 35 40 45Arg Lys Gln Leu Arg Leu Tyr
Gln Leu Tyr Ser Arg Thr Ser Gly Lys 50 55 60His Ile Gln Val Leu Gly
Arg Arg Ile Ser Ala Arg Gly Glu Asp Gly65 70 75 80Asp Lys Tyr Ala
Gln Leu Leu Val Glu Thr Asp Thr Phe Gly Ser Gln 85 90 95Val Arg Ile
Lys Gly Lys Glu Thr Glu Phe Tyr Leu Cys Met Asn Arg 100 105 110Lys
Gly Lys Leu Val Gly Lys Pro Asp Gly Thr Ser Lys Glu Cys Val 115 120
125Phe Ile Glu Lys Val Leu Glu Asn Asn Tyr Thr Ala Leu Met Ser Ala
130 135 140Lys Tyr Ser Gly Trp Tyr Val Gly Phe Thr Lys Lys Gly Arg
Pro Arg145 150 155 160Lys Gly Pro Lys Thr Arg Glu Asn Gln Gln Asp
Val His Phe Met Lys 165 170 175Arg Tyr Pro Lys Gly Gln Pro Glu Leu
Gln Lys Pro Phe Lys Tyr Thr 180 185 190Thr Val Thr Lys Arg Ser Arg
Arg Ile Arg Pro Thr His Pro Ala 195 200 205405PRTArtificial
SequenceSITE(1)...(5)Bovine enterokinase cleavage site 40Asp Asp
Asp Asp Lys1 5417PRTArtificial
SequenceSITE(1)...(7)Tobacco Etch Virus cleavage site 41Glu Asn Leu
Tyr Phe Gln Gly1 5427PRTArtificial SequenceSITE(1)...(7)Tobacco
Etch Virus cleavage site 42Glu Asn Leu Tyr Phe Gln Ser1
5437PRTArtificial SequenceSITE(1)...(7)Tobacco Etch Virus cleavage
site 43Glu Asn Ile Tyr Thr Gln Gly1 5447PRTArtificial
SequenceSITE(1)...(7)Tobacco Etch Virus cleavage site 44Glu Asn Ile
Tyr Thr Gln Ser1 5457PRTArtificial SequenceSITE(1)...(7)Tobacco
Etch Virus cleavage site 45Glu Asn Ile Tyr Leu Gln Gly1
5467PRTArtificial SequenceSITE(1)...(7)Tobacco Etch Virus cleavage
site 46Glu Asn Ile Tyr Leu Gln Ser1 5477PRTArtificial
SequenceSITE(1)...(7)Tobacco Etch Virus cleavage site 47Glu Asn Val
Tyr Phe Gln Gly1 5487PRTArtificial SequenceSITE(1)...(7)Tobacco
Etch Virus cleavage site 48Glu Asn Val Tyr Ser Gln Ser1
5497PRTArtificial SequenceSITE(1)...(7)Tobacco Etch Virus cleavage
site 49Glu Asn Val Tyr Ser Gln Gly1 5507PRTArtificial
SequenceSITE(1)...(7)Tobacco Etch Virus cleavage site 50Glu Asn Val
Tyr Ser Gln Ser1 5517PRTArtificial SequenceSITE(1)...(7)Human
Rhinovirus 3C cleavage site 51Glu Ala Leu Phe Gln Gly Pro1
5527PRTArtificial SequenceSITE(1)...(7)Human Rhinovirus 3C cleavage
site 52Glu Val Leu Phe Gln Gly Pro1 5537PRTArtificial
SequenceSITE(1)...(7)Human Rhinovirus 3C cleavage site 53Glu Leu
Leu Phe Gln Gly Pro1 5547PRTArtificial SequenceSITE(1)...(7)Human
Rhinovirus 3C cleavage site 54Asp Ala Leu Phe Gln Gly Pro1
5557PRTArtificial SequenceSITE(1)...(7)Human Rhinovirus 3C cleavage
site 55Asp Val Leu Phe Gln Gly Pro1 5567PRTArtificial
SequenceSITE(1)...(7)Human Rhinovirus 3C cleavage site 56Asp Leu
Leu Phe Gln Gly Pro1 55798PRTArtificial
SequenceSITE(1)...(98)SUMO/ULP-1 cleavage site 57Met Ala Asp Ser
Glu Val Asn Gln Glu Ala Lys Pro Glu Val Lys Pro1 5 10 15Glu Val Lys
Pro Glu Thr His Ile Asn Leu Lys Val Ser Asp Gly Ser 20 25 30Ser Glu
Ile Phe Phe Lys Ile Lys Lys Thr Thr Pro Leu Arg Arg Leu 35 40 45Met
Glu Ala Phe Ala Lys Arg Gln Gly Lys Glu Met Asp Ser Leu Arg 50 55
60Phe Leu Tyr Asp Gly Ile Arg Ile Gln Ala Asp Gln Thr Pro Glu Asp65
70 75 80Leu Asp Met Glu Asp Asn Asp Ile Ile Glu Ala His Arg Glu Gln
Ile 85 90 95Gly Gly584PRTArtificial SequenceSITE(1)...(4)Thrombin
cleavage site 58Gly Val Arg Gly1594PRTArtificial
SequenceSITE(1)...(4)Thrombin cleavage site 59Ser Ala Arg
Gly1604PRTArtificial SequenceSITE(1)...(4)Thrombin cleavage site
60Ser Leu Arg Gly1614PRTArtificial SequenceSITE(1)...(4)Thrombin
cleavage site 61Asp Gly Arg Ile1624PRTArtificial
SequenceSITE(1)...(4)Thrombin cleavage site 62Gln Gly Lys
Ile1636PRTArtificial SequenceSITE(1)...(6)Thrombin cleavage site
63Leu Val Pro Arg Gly Ser1 5646PRTArtificial
SequenceSITE(1)...(6)Thrombin cleavage site 64Leu Val Pro Lys Gly
Ser1 5656PRTArtificial SequenceSITE(1)...(6)Thrombin cleavage site
65Phe Ile Pro Arg Thr Phe1 5666PRTArtificial
SequenceSITE(1)...(6)Thrombin cleavage site 66Val Leu Pro Arg Ser
Phe1 5676PRTArtificial SequenceSITE(1)...(6)Thrombin cleavage site
67Ile Val Pro Arg Ser Phe1 5686PRTArtificial
SequenceSITE(1)...(6)Thrombin cleavage site 68Ile Val Pro Arg Gly
Tyr1 5696PRTArtificial SequenceSITE(1)...(6)Thrombin cleavage site
69Val Val Pro Arg Gly Val1 5706PRTArtificial
SequenceSITE(1)...(6)Thrombin cleavage site 70Val Leu Pro Arg Leu
Ile1 5716PRTArtificial SequenceSITE(1)...(6)Thrombin cleavage site
71Val Met Pro Arg Ser Leu1 5726PRTArtificial
SequenceSITE(1)...(6)Thrombin cleavage site 72Met Phe Pro Arg Ser
Leu1 5734PRTArtificial SequenceSITE(1)...(4)Coagulation Factor Xa
cleavage site 73Ile Asp Gly Arg1744PRTArtificial
SequenceSITE(1)...(4)Coagulation Factor Xa cleavage site 74Ile Glu
Gly Arg1755PRTArtificial SequenceDOMAIN(1)...(5)Flexible spacer
75Gly Gly Gly Gly Ser1 5765PRTArtificial
SequenceDOMAIN(1)...(5)Flexible spacer 76Glu Ala Ala Ala Lys1
5778PRTArtificial SequencePEPTIDE(1)...(8)FLAG epitope-binding
region 77Asp Tyr Lys Asp Asp Asp Asp Lys1 5789PRTArtificial
SequencePEPTIDE(1)...(9)Human Influenza virus hemagluttinin (HA)
epitope-binding region 78Tyr Pro Tyr Asp Val Pro Asp Tyr Ala1
57910PRTArtificial SequencePEPTIDE(1)...(10)Human p62 c-Myc
epitope-binding region 79Glu Gln Lys Leu Ile Ser Glu Glu Asp Leu1 5
108011PRTArtificial SequencePEPTIDE(1)...(11)Vesicular Stomatitis
Virus Glycoprotein (VSV-G) epitope-binding region 80Tyr Thr Asp Ile
Glu Met Asn Arg Leu Gly Lys1 5 10816PRTArtificial
SequencePEPTIDE(1)...(6)Substance P epitope-binding region 81Gln
Phe Phe Gly Leu Met1 58211PRTArtificial
SequencePEPTIDE(1)...(11)Glycoprotein-D precursor of Herpes simplex
virus epitope-binding region 82Gln Pro Glu Leu Ala Pro Glu Asp Pro
Glu Asp1 5 108314PRTArtificial SequencePEPTIDE(1)...(14)V5
epitope-binding region 83Gly Lys Pro Ile Pro Asn Pro Leu Leu Gly
Leu Asp Ser Thr1 5 10846PRTArtificial SequencePEPTIDE(1)...(6)AU1
epitope-binding region 84Asp Thr Tyr Arg Tyr Ile1 5856PRTArtificial
SequencePEPTIDE(1)...(6)AU5 epitope-binding region 85Thr Asp Phe
Tyr Leu Lys1 5866PRTArtificial SequencePEPTIDE(1)...(6)HIS
epitope-binding region 86His His His His His His1 58723PRTInfluenza
A virus 87Gly Leu Phe Gly Ala Ile Ala Gly Phe Ile Glu Asn Gly Trp
Glu Gly1 5 10 15Met Ile Asp Gly Trp Tyr Gly 208823PRTInfluenza A
virus 88Gly Ile Phe Gly Ala Ile Ala Gly Phe Ile Glu Asn Gly Trp Glu
Gly1 5 10 15Met Ile Asp Gly Trp Tyr Gly 208923PRTInfluenza A virus
89Gly Leu Phe Gly Ala Ile Ala Gly Phe Ile Glu Asn Gly Trp Glu Gly1
5 10 15Met Val Asp Gly Trp Tyr Gly 209023PRTInfluenza A virus 90Gly
Ile Phe Gly Ala Ile Ala Gly Phe Ile Glu Asn Gly Trp Glu Gly1 5 10
15Met Val Asp Gly Trp Tyr Gly 209123PRTInfluenza A virus 91Gly Leu
Phe Gly Ala Ile Ala Gly Phe Ile Glu Asn Gly Trp Glu Gly1 5 10 15Leu
Ile Asp Gly Trp Tyr Gly 209226PRTSemliki Forest virus 92Asp Tyr Gln
Cys Lys Val Tyr Thr Gly Val Tyr Pro Phe Met Trp Gly1 5 10 15Gly Ala
Tyr Cys Phe Cys Asp Ser Glu Asn 20 259326PRTGetah virus 93Asp Tyr
Gln Cys Gln Val Tyr Thr Gly Val Tyr Pro Phe Met Trp Gly1 5 10 15Gly
Ala Tyr Cys Phe Cys Asp Thr Glu Asn 20 259426PRTEastern equine
encephalitis virus 94Asp Tyr Gln Cys Gln Val Phe Thr Gly Val Tyr
Pro Phe Met Trp Gly1 5 10 15Gly Ala Tyr Cys Phe Cys Asp Thr Glu Asn
20 259526PRTEastern equine encephalitis virus 95Asp Tyr Gln Cys Gln
Val Phe Ser Gly Val Tyr Pro Phe Met Trp Gly1 5 10 15Gly Ala Tyr Cys
Phe Cys Asp Thr Glu Asn 20 259626PRTEastern equine encephalitis
virus 96Asp Tyr Gln Cys Gln Val Phe Thr Gly Ser Tyr Pro Phe Met Trp
Gly1 5 10 15Gly Ala Tyr Cys Phe Cys Asp Thr Glu Asn 20
259726PRTEastern equine encephalitis virus 97Asp Tyr Gln Cys Gln
Val Phe Thr Gly Val Tyr Pro Phe Met Trp Gly1 5 10 15Gly Ala Tyr Cys
Phe Cys Asp Thr Glu Asp 20 259826PRTEastern equine encephalitis
virus 98Asp Tyr Gln Cys Gln Val Phe Ala Gly Val Tyr Pro Phe Met Trp
Gly1 5 10 15Gly Ala Tyr Cys Phe Cys Asp Thr Glu Asn 20
259926PRTEastern equine encephalitis virus 99Asp Tyr Gln Cys Gln
Val Phe Ala Gly Ile Tyr Pro Phe Met Trp Gly1 5 10 15Gly Ala Tyr Cys
Phe Cys Asp Thr Glu Asn 20 2510026PRTChikungunya virus 100Asp Tyr
Ser Cys Lys Val Phe Thr Gly Val Tyr Pro Phe Met Trp Gly1 5 10 15Gly
Ala Tyr Cys Phe Cys Asp Thr Glu Asn 20 2510126PRTO'nyong-nyong
virus 101Asp Tyr Asn Cys Lys Val Phe Thr Gly Val Tyr Pro Phe Met
Trp Gly1 5 10 15Gly Ala Tyr Cys Phe Cys Asp Ala Glu Asn 20
2510226PRTVenezuelan equine encephalitis virus 102Asp Glu Gln Cys
Lys Val Phe Thr Gly Val Tyr Pro Phe Met Trp Gly1 5 10 15Gly Ala Tyr
Cys Phe Cys Asp Ser Glu Asn 20 2510326PRTVenezuelan equine
encephalitis virus 103Asp Glu Gln Cys Lys Val Phe Thr Gly Val Tyr
Pro Phe Met Trp Gly1 5 10 15Gly Ala Tyr Cys Phe Cys Asp Ala Glu Asn
20 2510426PRTVenezuelan equine encephalitis virus 104Asp Glu Gln
Cys Gln Val Phe Thr Gly Val Tyr Pro Phe Met Trp Gly1 5 10 15Gly Ala
Tyr Cys Phe Cys Asp Ser Glu Asn 20 2510526PRTVenezuelan equine
encephalitis virus 105Asp Glu Gln Cys Lys Val Phe Thr Gly Val Tyr
Pro Phe Met Trp Gly1 5 10 15Gly Ala Tyr Cys Phe Cys Asp Thr Glu Asn
20 2510626PRTVenezuelan equine encephalitis virus 106Asp Glu Gln
Cys Lys Val Phe Ser Gly Val Tyr Pro Phe Met Trp Gly1 5 10 15Gly Ala
Tyr Cys Phe Cys Asp Thr Glu Asn 20 2510726PRTVenezuelan equine
encephalitis virus 107Asp Glu Lys Cys Lys Val Phe Thr Gly Val Tyr
Pro Phe Met Trp Gly1 5 10 15Gly Ala Tyr Cys Phe Cys Asp Thr Glu Asn
20 2510826PRTVenezuelan equine encephalitis virus 108Asp Glu His
Cys Lys Val Phe Thr Gly Val Tyr Pro Phe Met Trp Gly1 5 10 15Gly Ala
Tyr Cys Phe Cys Asp Thr Glu Asn 20 2510926PRTVenezuelan equine
encephalitis virus 109Asp Glu Gln Cys Lys Val Phe Ala Gly Val Tyr
Pro Phe Met Trp Gly1 5 10 15Gly Ala Tyr Cys Phe Cys Asp Thr Glu Asn
20 2511026PRTUna virus 110Asp Tyr Arg Cys Gln Thr Tyr Thr Gly Val
Tyr Pro Phe Leu Trp Gly1 5 10 15Gly Ala Tyr Cys Phe Cys Asp Thr Glu
Asn 20 2511126PRTMayaro virus 111Glu Tyr Lys Cys Ala Val Phe Thr
Gly Val Tyr Pro Phe Met Trp Gly1 5 10 15Gly Ala Tyr Cys Phe Cys Asp
Ser Glu Asn 20 2511226PRTBarmah Forest virus 112Asp Tyr Lys Cys Ser
Val Phe Thr Gly Val Tyr Pro Phe Met Trp Gly1 5 10 15Gly Ala Tyr Cys
Phe Cys Asp Thr Glu Asn 20 2511326PRTAura virus 113Asp Tyr Thr Cys
Lys Val Phe Thr Gly Val Tyr Pro Phe Leu Trp Gly1 5 10 15Gly Ala Gln
Cys Phe Cys Asp Ser Glu Asn 20 2511426PRTNdumu virus 114Asp Tyr Gln
Cys Arg Val Asp Ser Gly Val Tyr Pro Tyr Met Trp Gly1 5 10 15Gly Ala
Tyr Cys Phe Cys Ser Ser Glu Asn 20 2511526PRTHighlands J virus
115Asp Tyr Thr Cys Arg Val Phe Gly Gly Val Tyr Pro Phe Met Trp Gly1
5 10 15Gly Ala Gln Cys Phe Cys Asp Ser Glu Asn 20 2511626PRTFort
Morgan virus 116Asp Tyr Ala Cys Arg Val Phe Gly Gly Val Tyr Pro Phe
Met Trp Gly1 5 10 15Gly Ala Gln Cys Phe Cys Asp Thr Glu Asn 20
2511726PRTSindbis virus 117Asp Tyr Asn Cys Lys Val Phe Gly Gly Val
Tyr Pro Phe Met Trp Gly1 5 10 15Gly Ala Gln Cys Phe Cys Asp Ser Glu
Asn 20 2511826PRTSindbis virus 118Asp Tyr Thr Cys Lys Val Phe Gly
Gly Val Tyr Pro Phe Met Trp Gly1 5 10 15Gly Ala Gln Cys Phe Cys Asp
Ser Glu Asn 20 2511921PRTVesicular stomatitis virus 119Val Ser Phe
Asn Pro Gly Phe Pro Pro Gln Ser Cys Gly Tyr Gly Thr1 5 10 15Val Thr
Asp Ala Glu 2012021PRTVesicular stomatitis virus 120Val Ser Phe Asn
Pro Gly Phe Pro Pro Gln Ser Cys Gly Tyr Gly Ser1 5 10 15Val Thr Asp
Ala Glu 2012121PRTVesicular stomatitis virus 121Val Gly Phe Asn Pro
Gly Phe Pro Pro Gln Ser Cys Gly Tyr Gly Thr1 5 10 15Val Thr Asp Ala
Glu 2012221PRTVesicular stomatitis virus 122Ile Ser Phe Asn Pro Gly
Phe Pro Pro Gln Ser Cys Gly Tyr Gly Ser1 5 10 15Val Thr Asp Ala Glu
2012321PRTVesicular stomatitis virus 123Ile Ser Leu Asn Pro Gly Phe
Pro Pro Gln Ser Cys Gly Tyr Gly Ser1 5 10 15Val Thr Asp Ala Glu
2012421PRTVesicular stomatitis virus 124Thr Trp Leu Asn Pro Gly Phe
Pro Pro Gln Ser Cys Gly Tyr Ala Thr1 5 10 15Val Thr Asp Ala Glu
2012521PRTVesicular stomatitis virus 125Ile Trp Ile Asn Pro Gly Phe
Pro Pro Gln Ser Cys Gly Tyr Ala Thr1 5 10 15Val Thr Asp Ala Glu
2012621PRTPiry virus 126Ser Leu Ile Asn Leu Gly Phe Pro Pro Glu Ser
Cys Gly Tyr Ala Thr1 5 10 15Val Thr Asp Ser Glu 2012721PRTIsfahan
virus 127Thr Leu Met Tyr Pro Gly Phe Pro Pro Glu Ser Cys Gly Tyr
Ala Ser1 5 10 15Ile Thr Asp Ser Glu 2012821PRTChandipura virus
128Thr Leu Val Ser Pro Gly Phe Pro Pro Glu Ser Cys Gly Tyr Ala Ser1
5 10 15Val Thr Asp Ser Glu 2012921PRTCocal virus 129Thr Trp Met Ser
Pro Gly Phe Pro Pro Gln Asn Cys Gly Tyr Ala Thr1 5 10 15Val Thr Asp
Ser Val 2013025PRTSendai virus 130Phe Phe Gly Ala Val Ile Gly Thr
Ile Ala Leu Gly Val Ala Thr Ser1 5 10 15Ala Gln Ile Thr Ala Gly Ile
Ala Leu 20 2513125PRTSendai virus 131Phe Phe Gly Ala Val Ile Gly
Thr Ile Ala Leu Gly Val Ala Thr Ser1 5 10 15Ala Gln Ile Thr Thr Gly
Ile Ala Leu 20 2513225PRTHuman parainfluenza virus 1 132Phe Phe Gly
Ala Val Ile Gly Thr Ile Ala Leu Gly Val Ala Thr Ala1 5 10 15Ala Gln
Ile Thr Ala Gly Ile Ala Leu 20 2513325PRTHuman parainfluenza virus
3 133Phe Phe Gly Gly Val Ile Gly Thr Ile Ala Leu Gly Val Ala Thr
Ser1 5 10 15Ala Gln Ile Thr Ala Ala Val Ala Leu 20
2513425PRTBeilong virus 134Phe Trp Gly Ala Ile Ile Gly Gly Val Ala
Leu Gly Val Ala Thr Ser1 5 10 15Ala Gln Ile Thr Ala Gly Val Ala Leu
20 2513525PRTMurayama virus 135Phe Val Gly Ala Ile Ile Gly Thr Val
Ala Leu Gly Val Ala Thr Ser1 5 10 15Ala Gln Ile Thr Ala Ala Val Ala
Leu 20 2513625PRTMossman virus 136Phe Phe Gly Ala Val Ile Ala Gly
Val Ala Leu Gly Val Ala Thr Ala1 5 10 15Ala Gln Val Thr Ala Gly Val
Ala Leu 20 2513725PRTCanine distemper virus 137Phe Ala Gly Val Val
Leu Ala Gly Val Ala Leu Gly Val Ala Thr Ala1 5 10 15Ala Gln Ile Thr
Ala Gly Ile Ala Leu 20 2513825PRTCanine distemper virus 138Phe Ala
Gly Val Val Leu Ala Gly Ala Ala Leu Gly Val Ala Thr Ala1 5 10 15Ala
Gln Ile Thr Ala Gly Ile Ala Leu 20
2513925PRTPeste-des-petits-ruminants virus 139Phe Val Gly Ala Val
Leu Ala Gly Val Ala Leu Gly Val Ala Thr Ala1 5 10 15Ala Gln Ile Thr
Ala Gly Val Ala Leu 20 2514025PRTPeste-des-petits-ruminants virus
140Phe Ala Gly Ala Val Leu Ala Gly Val Ala Leu Gly Val Ala Thr Ala1
5 10 15Ala Gln Ile Thr Ala Gly Val Ala Leu 20
2514125PRTPeste-des-petits-ruminants virus 141Phe Ala Gly Ala Val
Leu Ala Gly Val Ala Leu Gly Val Ala Thr Arg1 5 10 15Ala Gln Ile Thr
Ala Gly Val Ala Leu 20 2514225PRTAvian paramyxovirus 2
142Phe Val Gly Ala Ile Ile Gly Ser Val Ala Leu Gly Val Ala Thr Ala1
5 10 15Ala Gln Ile Thr Ala Ala Val Ala Leu 20 2514325PRTPhocine
distemper virus 143Phe Ala Gly Val Val Ile Ala Gly Ala Ala Leu Gly
Val Ala Thr Ala1 5 10 15Ala Gln Ile Thr Ala Gly Val Ala Leu 20
2514425PRTSimian virus 5 144Phe Ala Gly Val Val Ile Gly Leu Ala Ala
Leu Gly Val Ala Thr Ala1 5 10 15Ala Gln Val Thr Ala Ala Val Ala Leu
20 2514525PRTSimian virus 41 145Phe Ala Gly Val Val Val Gly Leu Ala
Ala Leu Gly Val Ala Thr Ala1 5 10 15Ala Gln Val Thr Ala Ala Val Ala
Val 20 2514625PRTDolphin morbillivirus 146Phe Ala Gly Val Val Leu
Ala Gly Val Ala Leu Gly Val Ala Thr Ala1 5 10 15Ala Gln Ile Thr Ala
Gly Val Ala Leu 20 2514725PRTNewcastle disease virus 147Phe Ile Gly
Ala Val Ile Gly Ser Val Ala Leu Gly Val Ala Thr Ser1 5 10 15Ala Gln
Ile Thr Ala Ala Ala Ala Leu 20 2514825PRTNewcastle disease virus
148Phe Ile Gly Ala Ile Ile Gly Ser Ile Ala Leu Gly Val Ala Thr Ser1
5 10 15Ala Gln Ile Thr Ala Ala Ala Ala Leu 20 2514925PRTNewcastle
disease virus 149Phe Ile Gly Ala Ile Ile Gly Ser Val Ala Leu Gly
Val Ala Thr Ser1 5 10 15Ala Gln Ile Thr Ala Ala Ala Ala Leu 20
2515025PRTNewcastle disease virus 150Phe Ile Gly Ala Ile Ile Gly
Ser Val Ala Leu Gly Ile Ala Thr Ser1 5 10 15Ala Gln Ile Thr Ala Ala
Ala Ala Leu 20 2515125PRTNewcastle disease virus 151Phe Ile Gly Ala
Val Ile Gly Ser Val Ala Leu Gly Val Ala Thr Ala1 5 10 15Val Gln Ile
Thr Ala Ala Ala Ala Leu 20 2515225PRTNewcastle disease virus 152Phe
Ile Gly Ala Ile Ile Gly Ser Val Ala Leu Gly Val Ala Thr Ala1 5 10
15Ala Gln Ile Thr Ala Ala Ser Ala Leu 20 2515325PRTNewcastle
disease virus 153Phe Ile Gly Ala Ile Ile Gly Ser Val Ala Leu Gly
Val Ala Thr Asp1 5 10 15Ala Gln Ile Thr Ala Ala Ser Ala Leu 20
2515425PRTNewcastle disease virus 154Phe Ile Gly Ala Ile Ile Gly
Ser Val Ala Leu Gly Val Ala Thr Pro1 5 10 15Ala Gln Ile Thr Ala Ala
Ser Ala Leu 20 2515525PRTNewcastle disease virus 155Phe Ile Gly Ala
Ile Ile Gly Gly Val Ala Leu Gly Val Ala Thr Ala1 5 10 15Ala Gln Ile
Thr Ala Ala Ser Ala Leu 20 2515625PRTNewcastle disease virus 156Phe
Ile Gly Ala Ile Ile Gly Ser Val Ala Leu Gly Val Ala Thr Ser1 5 10
15Ala Gln Ile Thr Ala Ala Thr Ala Leu 20 2515725PRTNewcastle
disease virus 157Leu Ile Gly Ala Ile Ile Gly Gly Val Ala Leu Gly
Val Ala Thr Ala1 5 10 15Ala Gln Ile Thr Ala Ala Ser Ala Leu 20
2515825PRTPigeon paramyxovirus-1 158Phe Ile Gly Ala Ile Ile Gly Ser
Val Ala Leu Gly Val Ala Lys Ser1 5 10 15Ala Gln Ile Thr Ala Ala Ala
Ala Leu 20 2515921PRTPneumonia virus 159Phe Leu Gly Leu Ile Leu Gly
Leu Gly Ala Ala Val Thr Ala Gly Val1 5 10 15Ala Leu Ala Lys Thr
2016025PRTHendra virus 160Leu Ala Gly Val Val Met Ala Gly Ile Ala
Ile Gly Ile Ala Thr Ala1 5 10 15Ala Gln Ile Thr Ala Gly Val Ala Leu
20 2516125PRTNipah virus 161Leu Ala Gly Val Ile Met Ala Gly Val Ala
Ile Gly Ile Ala Thr Ala1 5 10 15Ala Gln Ile Thr Ala Gly Val Ala Leu
20 2516225PRTHuman metapneumovirus 162Gln Ser Arg Phe Val Leu Gly
Ala Ile Ala Leu Gly Val Ala Thr Thr1 5 10 15Ala Ala Val Thr Ala Gly
Ile Ala Ile 20 25
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