U.S. patent application number 12/906295 was filed with the patent office on 2011-02-03 for adhesion promoting primer for coated surfaces.
Invention is credited to Vipul Dave, Robert Falotico, Chengxue Li, Thai M. Nguyen, Theodore L. Parker, Jonathon Z. Zhao.
Application Number | 20110029070 12/906295 |
Document ID | / |
Family ID | 41683629 |
Filed Date | 2011-02-03 |
United States Patent
Application |
20110029070 |
Kind Code |
A1 |
Dave; Vipul ; et
al. |
February 3, 2011 |
ADHESION PROMOTING PRIMER FOR COATED SURFACES
Abstract
An expandable medical device includes a plurality of elongated
struts, forming a substantially cylindrical device which is
expandable from a first diameter to a second diameter. A plurality
of different beneficial agents may be loaded into different
openings within the struts for delivery to the tissue. For
treatment of conditions such as restenosis, different agents are
loaded into different openings in the device to address different
biological processes involved in restenosis and are delivered at
different release kinetics matched to the biological process
treated. The different agents may also be used to address different
diseases from the same drug delivery device. In addition,
anti-thrombotic agents may be affixed to at least a portion of the
surfaces of the medical device for the prevention of sub-acute
thrombosis. To ensure that the different agents remain affixed to
the device as well as to each other, primer layers may be
utilized.
Inventors: |
Dave; Vipul; (Hillsborough,
NJ) ; Falotico; Robert; (Belle Mead, NJ) ; Li;
Chengxue; (Fremont, CA) ; Nguyen; Thai M.;
(Santa Clara, CA) ; Parker; Theodore L.;
(Danville, CA) ; Zhao; Jonathon Z.; (Belie Mead,
NJ) |
Correspondence
Address: |
PHILIP S. JOHNSON;JOHNSON & JOHNSON
ONE JOHNSON & JOHNSON PLAZA
NEW BRUNSWICK
NJ
08933-7003
US
|
Family ID: |
41683629 |
Appl. No.: |
12/906295 |
Filed: |
October 18, 2010 |
Related U.S. Patent Documents
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Application
Number |
Filing Date |
Patent Number |
|
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12335741 |
Dec 16, 2008 |
7819914 |
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12906295 |
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Current U.S.
Class: |
623/1.46 ;
427/2.25 |
Current CPC
Class: |
A61L 31/16 20130101;
A61L 31/10 20130101; A61F 2250/0043 20130101; A61F 2/915 20130101;
A61F 2250/0068 20130101; A61F 2002/91558 20130101; A61F 2/91
20130101 |
Class at
Publication: |
623/1.46 ;
427/2.25 |
International
Class: |
A61F 2/82 20060101
A61F002/82; B05D 7/00 20060101 B05D007/00 |
Claims
1. (canceled)
2. (canceled)
3. (canceled)
4. (canceled)
5. (canceled)
6. (canceled)
7. (canceled)
8. (canceled)
9. (canceled)
10. (canceled)
11. (canceled)
12. An implantable medical device comprising: a substantially
cylindrical intraluminal scaffold which is expandable from a first
diameter for delivery into a vessel, to a second diameter for
expanding the vessel, the intraluminal scaffold having a luminal
surface and an abluminal surface, the distance between the luminal
surface and the abluminal surface defining the wall thickness of
the intraluminal scaffold, the intraluminal scaffold also including
a plurality of openings extending from the luminal to abluminal
surface; a first coating comprising a material having a first
electric charge affixed to at least a portion of the abluminal
surface, the luminal surface and a surface of the plurality of
openings; a second coating comprising a material having a second
electric charge affixed to at least a portion of the first coating,
the second electric charge being opposite of the first electric
charge; and at least one therapeutic agent deposited in at least
one of the plurality of openings, wherein the second coating is
configured as an intermediate bonding layer between the first
coating and the at least one therapeutic agent.
13. A method of coating an intraluminal scaffold having a plurality
of openings therein, the method comprising applying a first coating
comprising a material having a first electric charge to at least a
portion of a surface of the intraluminal scaffold and a surface of
the plurality of openings; applying a second coating comprising a
material having a second electric charge to at least a portion of
the first coating, the second electric charge being opposite of the
first electric charge; and applying at least one therapeutic agent
into at least one of the plurality of openings.
14. The implantable medical device, according to claim 1, wherein
the second coating comprises a polymeric polycation.
Description
BACKGROUND OF THE INVENTION
[0001] 1. Field of the Invention
[0002] The present invention relates to tissue-supporting medical
devices, and more particularly to expandable, non-removable devices
that are implanted within a bodily lumen of a living animal or
human to support the organ and maintain patency, and that have
openings for delivery of a plurality of beneficial agents to the
intervention site as well as a surface coating of an antithrombotic
agent. The present invention also relates to primer coatings for
use between the antithrombotic agent coating and other therapeutic
agent/polymer matrices.
[0003] 2. Discussion of the Related Art
[0004] In the past, permanent or biodegradable devices have been
developed for implantation within a body passageway to maintain
patency of the passageway. These devices are typically introduced
percutaneously, and transported transluminally until positioned at
a desired location. These devices are then expanded either
mechanically, such as by the expansion of a mandrel or balloon
positioned inside the device, or expand themselves by releasing
stored energy upon actuation within the body. Once expanded within
the lumen, these devices, called stents, become encapsulated within
the body tissue and remain a permanent implant.
[0005] Known stent designs include monofilament wire coil stents
(U.S. Pat. No. 4,969,458); welded metal cages (U.S. Pat. Nos.
4,733,665 and 4,776,337); and, most prominently, thin-walled metal
cylinders with axial slots formed around the circumference (U.S.
Pat. Nos. 4,733,665; 4,739,762; and 4,776,337). Known construction
materials for use in stents include polymers, organic fabrics and
biocompatible metals, such as, stainless steel, gold, silver,
tantalum, titanium, and shape memory alloys, such as
nickel-titanium.
[0006] U.S. Pat. No. 6,241,762, which is incorporated herein by
reference in its entirety, discloses a non-prismatic stent design
which remedies several performance deficiencies of previous stents.
In addition, preferred embodiments disclosed in this patent provide
a stent with large, non-deforming strut and link elements, which
may contain holes without compromising the mechanical properties of
the strut or link elements, or the device as a whole. Further,
these holes may serve as large, protected reservoirs for delivering
various beneficial agents to the device implantation site without
the need for a surface coating on the stent.
[0007] Of the many problems that may be addressed through
stent-based local delivery of beneficial agents, one of the most
important is restenosis. Restenosis is a major complication that
may arise following vascular interventions such as angioplasty and
the implantation of stents. Simply defined, restenosis is a wound
healing process that reduces the vessel lumen diameter by
extracellular matrix deposition and vascular smooth muscle cell
proliferation and which may ultimately result in renarrowing or
even reocclusion of the lumen. Despite the introduction of improved
surgical techniques, devices and pharmaceutical agents, the overall
restenosis rate for bare metal stents is still reported in the
range of about twenty-five percent to about fifty percent within
six to twelve months after an angioplasty procedure. To treat this
condition, additional revascularization procedures are frequently
required, thereby increasing trauma and risk to the patient.
[0008] Conventional stents with surface coatings of various
beneficial agents have shown promising results in reducing
restenosis. U.S. Pat. No. 5,716,981, for example, discloses a stent
that is surface-coated with a composition comprising a polymer
carrier and paclitaxel. The patent offers detailed descriptions of
methods for coating stent surfaces, such as spraying and dipping,
as well as the desired character of the coating itself: it should
"coat the stent smoothly and evenly" and "provide a uniform,
predictable, prolonged release of the anti-angiogenic factor."
Surface coatings, however, may provide little actual control over
the release kinetics of beneficial agents. These coatings are
necessarily very thin, typically five to eight microns deep. The
surface area of the stent, by comparison is very large, so that the
entire volume of the beneficial agent has a very short diffusion
path to discharge into the surrounding tissue. The resulting
cumulative drug release profile is characterized by a large initial
burst, followed by a rapid approach to an asymptote, rather than
the desired "uniform, prolonged release," or linear release.
[0009] Increasing the thickness of the surface coating has the
beneficial effects of improving drug release kinetics including the
ability to better control drug release and to allow increased drug
loading. However, the increased coating thickness results in an
increased overall thickness of the stent wall. This is undesirable
for a number of reasons, including potential increased trauma to
the vessel lumen during implantation, reduced flow cross-section of
the lumen after implantation, and increased vulnerability of the
coating to mechanical failure or damage during expansion and
implantation. Coating thickness is one of several factors that
affect the release kinetics of the beneficial agent, and
limitations on thickness thereby limit the range of release rates,
durations, and the like that may be achieved.
[0010] Surface coatings may also limit the delivery of multiple
drugs from a stent. For example, if multiple drugs were to be
released from a surface coating, the release rates, delivery
periods and other release characteristics may not be independently
controlled in a facile way. However, restenosis involves multiple
biological processes and may be treated most effectively by a
combination of drugs selected to act on these different biological
processes.
[0011] A paper entitled "Physiological Transport Forces Govern Drug
Distribution for Stent-Based Delivery" by Chao-Wei Hwang et al. has
revealed an important interrelationship between the spatial and
temporal drug distribution properties of drug eluting stents, and
cellular drug transport mechanisms. In pursuit of enhanced
mechanical performance and structural properties, stent designs
have evolved to more complex geometries with inherent inhomogeneity
in the circumferential and longitudinal distribution of stent
struts. Examples of this trend are the typical commercially
available stents which expand to a roughly diamond or polygonal
shape when deployed in a bodily lumen. Both have been used to
deliver a beneficial agent in the form of a surface coating.
Studies have shown that lumen tissue portions immediately adjacent
to the struts acquire much higher concentrations of drug than more
remote tissue portions, such as those located in the middle of the
"diamond" shaped strut cells. Significantly, this concentration
gradient of drug within the lumen wall remains higher over time for
hydrophobic beneficial agents, such as paclitaxel or a rapamycin,
which have proven to be the most effective anti-restinotics to
date. Because local drug concentrations and gradients are
inextricably linked to biological effects, the initial spatial
distribution of the beneficial agent sources (the stent struts) is
key to efficacy.
[0012] In addition to the sub-optimal spatial distribution of
beneficial agents, there are further potential disadvantages with
surface coated stents. Certain fixed matrix polymer carriers
frequently used in the device coatings typically retain a
significant percent of the beneficial agent in the coating
indefinitely. Since these beneficial agents may be cytotoxic, for
example, paclitaxel, sub-acute and chronic problems such as chronic
inflammation, late thrombosis, and late or incomplete healing of
the vessel wall may occur. Additionally, the carrier polymers
themselves are often inflammatory to the tissue of the vessel wall.
On the other hand, the use of bio-degradable polymer carriers on
stent surfaces may result in "mal-apposition" or voids between the
stent and tissue of the vessel wall after the polymer carrier has
degraded. The voids permit differential motion between the stent
and adjacent tissue. Resulting problems include micro-abrasion and
inflammation, stent drift, and failure to re-endothelialize the
vessel wall.
[0013] Early human clinical trials suggest that there may be
certain disadvantages associated with first generation drug
delivery devices. Follow-up examination of clinical trial patients
at six to eighteen months after drug coated stent implantation
indicates that mal-apposition of stent struts to arterial walls and
edge effect restenosis may occur in significant numbers of
patients. Edge effect restenosis occurs just beyond the proximal
and distal edges of the stent and progresses around the stent edges
and into the interior (luminal) space, frequently requiring repeat
revascularization of the patient.
[0014] Another potential disadvantage is that expansion of the
stent may stress an overlying polymeric coating causing the coating
to peel, crack, or rupture which may effect drug release kinetics
or have other untoward effects. These effects have been observed in
first generation drug coated stents when these stents are expanded
to larger diameters, preventing their use thus far in larger
diameter arteries. Further, expansion of such a coated stent in an
atherosclerotic blood vessel will place circumferential shear
forces on the polymeric coating, which may cause the coating to
separate from the underlying stent surface. Such separation may
again have untoward effects including embolization of coating
fragments causing vascular obstruction.
[0015] Another problem that may be addressed through stent-based
local delivery of beneficial agents is thrombosis. A stent may be
coated with an anti-thrombotic agent in addition to one or more
therapeutic agents for treating restenosis. However, depending on
the coatings on the surface of the stent, for example, an
antithrombotic drug coating, additional layer(s) or primer layer(s)
may be preferable to enhance the adhesion of other therapeutic
agents to the coated surfaces of the stent.
SUMMARY OF THE INVENTION
[0016] The adhesion promoting primer for heparin coated surfaces of
the present invention overcomes the difficulties briefly described
above.
[0017] In accordance with one aspect, the present invention is
directed to an implantable medical device. The implantable medical
device comprising an intraluminal scaffold having a plurality of
openings therein, a first coating comprising a material having a
first electric charge affixed to at least a portion of a surface of
the intraluminal scaffold and a surface of the plurality of
openings, a second coating comprising a material having a second
electric charge affixed to at least a portion of the first coating,
the second electric charge being opposite of the first electric
charge, and at least one therapeutic agent deposited in at least
one of the plurality of openings, wherein the second coating is
configured as an intermediate layer between the first coating and
the at least one therapeutic agent.
[0018] In accordance with another aspect, the present invention is
directed to an implantable medical device. The implantable medical
device comprising a substantially cylindrical intraluminal scaffold
which is expandable from a first diameter for delivery into a
vessel, to a second diameter for expanding the vessel, the
intraluminal scaffold having a luminal surface and an abluminal
surface, the distance between the luminal surface and the abluminal
surface defining the wall thickness of the intraluminal scaffold,
the intraluminal scaffold also including a plurality of openings
extending from the luminal to abluminal surface, a first coating
comprising a material having a first electric charge affixed to at
least a portion of the abluminal surface, the luminal surface and a
surface of the plurality of openings, a second coating comprising a
material having a second electric charge affixed to at least a
portion of the first coating, the second electric charge being
opposite of the first electric charge, and at least one therapeutic
agent deposited in at least one of the plurality of openings,
wherein the second coating is configured as an intermediate bonding
layer between the first coating and the at least one therapeutic
agent.
[0019] In accordance with another aspect, the present invention is
directed to a method for coating an intraluminal scaffold having a
plurality of openings therein. The method comprising applying a
first coating comprising a material having a first electric charge
to at least a portion of a surface of the intraluminal scaffold and
a surface of the plurality of openings, applying a second coating
comprising a material having a second electric charge to at least a
portion of the first coating, the second electric charge being
opposite of the first electric charge, and applying at least one
therapeutic agent into at least one of the plurality of
openings.
[0020] In view of the drawbacks of the prior art, it would be
advantageous to provide a stent capable of delivering a relatively
large volume of a beneficial agent to a traumatized site in a
vessel lumen while avoiding the numerous potential problems
associated with surface coatings containing beneficial agents,
without increasing the effective wall thickness of the stent, and
without adversely impacting the mechanical expansion properties of
the stent.
[0021] It would further be advantageous to provide a tissue
supporting device with different beneficial agents provided in
different holes to achieve a desired spatial distribution of two or
more beneficial agents.
[0022] It would further be advantageous to provide a tissue
supporting device with different beneficial agents provided in
different holes to achieve a desired different release kinetic for
two different beneficial agents from the same device.
[0023] It would further be advantageous to provide a tissue
supporting device having all surfaces coated with an
anti-thrombotic agent and then utilize a primer in the holes or
openings therein to increase the adhesion of the one or more
beneficial agents that fill the holes.
[0024] The present invention is directed to primer compositions and
configurations for improving the adhesion of a drug delivery
matrix, e.g. therapeutic agent and polymer combination, to openings
of a medical device, for example, a stent, that have a heparin
coating. The present invention is particularly advantageous where
the heparin coating is covalently bonded to a metallic or polymer
surface of the medical device. In the present invention, the primer
preferably comprises a high molecular weight component or a low
molecular weight component, and the drug delivery matrix comprises
a drug and/or other beneficial agent and an excipient, preferably a
polymeric excipient. In addition, the primer may also preferably
comprise a material having an opposite charge with similar density
to that of the underlying layer, for example, heparin.
BRIEF DESCRIPTION OF THE DRAWINGS
[0025] The foregoing and other features and advantages of the
invention will be apparent from the following more particular
description of preferred embodiments of the invention as
illustrated in the accompanying drawings.
[0026] FIG. 1 is an isometric view of an expandable medical device
with a beneficial agent at the ends in accordance with the present
invention.
[0027] FIG. 2 is an isometric view of an expandable medical device
with a beneficial agent at a central portion and no beneficial
agent at the ends in accordance with the present invention.
[0028] FIG. 3 is an isometric view of an expandable medical device
with different beneficial agents in different holes in accordance
with the present invention.
[0029] FIG. 4 is an isometric view of an expandable medical device
with different beneficial agents in alternating holes in accordance
with the present invention.
[0030] FIG. 5 is an enlarged side view of a portion of an
expandable medical device with beneficial agent openings in the
bridging elements in accordance with the present invention.
[0031] FIG. 6 is an enlarged side view of a portion of an
expandable medical device with a bifurcation opening in accordance
with the present invention.
[0032] FIG. 7 is a cross sectional view of an expandable medical
device having a combination of a first agent, such as an
anti-inflammatory agent, in a first plurality of holes and a second
agent, such as an anti-proliferative agent, in a second plurality
of holes in accordance with the present invention.
[0033] FIG. 8 is a graph of the release rates of one example of an
anti-inflammatory and an anti-proliferative delivered by the
expandable medical device of FIG. 7 in accordance with the present
invention.
[0034] FIGS. 9A-9C are partial diagrammatic representations of an
alternate exemplary embodiment of an expandable medical device in
accordance with the present invention.
[0035] FIG. 10 illustrates a conjugation reaction between PLGA with
a carboxylic acid end group and low molecular weight PEI in
accordance with the present invention.
[0036] FIG. 11 illustrates a conjugation reaction between PLGA with
a carboxylic acid end group and high molecular weight or branched
PEI in accordance with the present invention.
DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS
[0037] FIG. 1 illustrates an expandable medical device having a
plurality of holes comprising a beneficial agent for delivery to
tissue by the expandable medical device. The expandable medical
device 10 illustrated in FIG. 1 is cut from a tube of material to
form a cylindrical expandable device. The expandable medical device
10 includes a plurality of cylindrical sections 12 interconnected
by a plurality of bridging elements 14. The bridging elements 14
allow the tissue supporting device to bend axially when passing
through the torturous path of vasculature to a deployment site and
allow the device to bend axially when necessary to match the
curvature of a lumen to be supported. Each of the cylindrical tubes
12 is formed by a network of elongated struts 18 which are
interconnected by ductile hinges 20 and circumferential struts 22.
During expansion of the medical device 10 the ductile hinges 20
deform while the struts 18 are not deformed. Further details of one
example of the expandable medical device are described in U.S. Pat.
No. 6,241,762 which is incorporated herein by reference in its
entirety.
[0038] As illustrated in FIG. 1, the elongated struts 18 and
circumferential struts 22 include openings 30, some of which
comprise a beneficial agent for delivery to the lumen in which the
expandable medical device is implanted. In addition, other portions
of the device 10, such as the bridging elements 14, may include
openings, as discussed below with respect to FIG. 5. Preferably,
the openings 30 are provided in non-deforming portions of the
device 10, such as the struts 18, so that the openings are
non-deforming and the beneficial agent is delivered without risk of
being fractured, expelled, or otherwise damaged during expansion of
the device. A further description of one example of the manner in
which the beneficial agent may be loaded within the openings 30 is
described in U.S. patent application Ser. No. 09/948,987, filed
Sep. 7, 2001, which is incorporated herein by reference in its
entirety.
[0039] The exemplary embodiments of the present invention may be
further refined by using Finite Element Analysis and other
techniques to optimize the deployment of the beneficial agents
within the openings 30. Basically, the shape and location of the
openings 30, may be modified to maximize the volume of the voids
while preserving the relatively high strength and rigidity of the
struts with respect to the ductile hinges 20. According to one
preferred exemplary embodiment of the present invention, the
openings have an area of at least 5.times.10.sup.-6 square inches,
and preferably at least 7.times.10.sup.-6 square inches. Typically,
the openings are filled, from about fifty percent to about
ninety-five percent full of beneficial agent.
DEFINITIONS
[0040] The terms "agent," "therapeutic agent" or "beneficial agent"
as used herein are intended to have the broadest possible
interpretation and are used to include any therapeutic agent or
drug, as well as inactive agents such as barrier layers, carrier
layers, therapeutic layers, or protective layers.
[0041] The terms "drug" and "therapeutic agent" are used
interchangeably to refer to any therapeutically active substance
that is delivered to a bodily lumen of a living being to produce a
desired, usually beneficial, effect. Beneficial agents may include
one or more drug or therapeutic agent.
[0042] The present invention is particularly well suited for the
delivery of antineoplastics, antiangiogenics, angiogenic factors,
anti-inflammatories, immuno-suppressants such as a rapamycin,
antirestenotics, antiplatelet agents, vasodilators,
anti-thrombotics, antiproliferatives, such as paclitaxel, for
example, and antithrombins, such as heparin, for example.
[0043] The term "erosion" means the process by which components of
a medium or matrix are bioresorbed and/or degraded and/or broken
down by chemical or physical or enzymatic processes. For example in
reference to biodegradable polymer matrices, erosion may occur by
cleavage or hydrolysis of the polymer chains, thereby increasing
the solubility of the matrix and suspended beneficial agents.
[0044] The term "erosion rate" is a measure of the amount of time
it takes for the erosion process to occur, usually reported in
unit-area per unit-time.
[0045] The terms "matrix" or "bioresorbable matrix" are used
interchangeably to refer to a medium or material that, upon
implantation in a subject, does not elicit a detrimental response
sufficient to result in the rejection of the matrix. The matrix
typically does not provide any therapeutic responses itself, though
the matrix may contain or surround a beneficial agent, as defined
herein. A matrix is also a medium that may simply provide support,
structural integrity or structural barriers. The matrix may be
polymeric, non-polymeric, hydrophobic, hydrophilic, lipophilic,
amphiphilic, and the like. In addition, bioresorbable matrix shall
also be understood to mean complete absorption of the matrix by the
body over time.
[0046] The term "openings" includes both through openings and
recesses.
[0047] The term "pharmaceutically acceptable" refers to the
characteristic of being non-toxic to a host or patient and suitable
for maintaining the stability of a beneficial agent and allowing
the delivery of the beneficial agent to target cells or tissue.
[0048] The term "polymer" refers to molecules formed from the
chemical union of two or more repeating units, called monomers.
Accordingly, included within the term "polymer" may be, for
example, dimers, trimers and oligomers. The polymer may be
synthetic, naturally-occurring or semisynthetic. In preferred form,
the term "polymer" refers to molecules which typically have a
M.sub.w greater than about 3000 and preferably greater than about
10,000 and a M.sub.w that is less than about 10 million, preferably
less than about a million and more preferably less than about
200,000. Examples of polymers include but are not limited to,
poly-.alpha.-hydroxy acid esters such as, polylactic acid (PLLA or
DLPLA), polyglycolic acid, polylactic-co-glycolic acid (PLGA),
polylactic acid-co-caprolactone; poly (block-ethylene
oxide-block-lactide-co-glycolide) polymers (PEO-block-PLGA and
PEO-block-PLGA-block-PEO); polyethylene glycol and polyethylene
oxide, poly (block-ethylene oxide-block-propylene
oxide-block-ethylene oxide); polyvinyl pyrrolidone;
polyorthoesters; polysaccharides and polysaccharide derivatives
such as polyhyaluronic acid, poly (glucose), polyalginic acid,
chitin, chitosan, chitosan derivatives, cellulose, methyl
cellulose, hydroxyethylcellulose, hydroxypropylcellulose,
carboxymethylcellulose, cyclodextrins and substituted
cyclodextrins, such as beta-cyclodextrin sulfobutyl ethers;
polypeptides and proteins, such as polylysine, polyglutamic acid,
albumin; polyanhydrides; polyhydroxy alkonoates such as polyhydroxy
valerate, polyhydroxy butyrate, and the like.
[0049] The term "primarily" with respect to directional delivery,
refers to an amount greater than about fifty percent of the total
amount of therapeutic agent provided to a blood vessel is provided
in the primary direction.
[0050] The various exemplary embodiments of the present invention
described herein provide different beneficial agents in different
openings in the expandable device or beneficial agent in some
openings and not in others. The particular structure of the
expandable medical device may be varied without departing from the
spirit of the invention. Since each opening is filled
independently, individual chemical compositions and pharmacokinetic
properties may be imparted to the beneficial agent in each
opening.
[0051] One example of the use of different beneficial agents in
different openings in an expandable medical device or beneficial
agents in some openings and not in others, is in addressing edge
effect restenosis. As discussed above, current generation coated
stents may have a difficulty with edge effect restenosis or
restenosis occurring just beyond the edges of the stent and
progressing around the stent and into the interior luminal
space.
[0052] The causes of edge effect restenosis in first generation
drug delivery stents are currently not well understood. It may be
that the region of tissue injury due to angioplasty and/or stent
implantation extends beyond the diffusion range of current
generation beneficial agents such as paclitaxel, which tends to
partition strongly in tissue. A similar phenomenon has been
observed in radiation therapies in which low doses of radiation at
the edges of stent have proven stimulatory in the presence of an
injury. In this case, radiating over a longer length until
uninjured tissue is irradiated solved the problem. In the case of
drug delivery stents, placing higher doses or higher concentrations
of beneficial agents along the stent edges, placing different
agents at the stent edges which diffuse more readily through the
tissue, or placing different beneficial agents or combinations of
beneficial agents at the edges of the device may help to remedy the
edge effect restenosis problem.
[0053] FIG. 1 illustrates an expandable medical device 10 with "hot
ends" or beneficial agent provided in the openings 30a at the ends
of the device in order to treat and reduce edge effect restenosis.
The remaining openings 30b in the central portion of the device may
be empty (as shown) or may contain a lower concentration of
beneficial agent.
[0054] Other mechanisms of edge effect restenosis may involve the
cytotoxicity of particular drugs or combinations of drugs. Such
mechanisms could include a physical or mechanical contraction of
tissue similar to that seen in epidermal scar tissue formation, and
the stent might prevent the contractile response within its own
boundaries, but not beyond its edges. Further, the mechanism of
this latter form of restenosis may be related to sequelae of
sustained or local drug delivery to the arterial wall that is
manifest even after the drug itself is no longer present in the
wall. That is, the restenosis may be a response to a form of
noxious injury related to the drug and/or the drug carrier. In this
situation, it might be beneficial to exclude certain agents from
the edges of the device.
[0055] FIG. 2 illustrates an alternate exemplary embodiment of an
expandable medical device 200 having a plurality of openings 230 in
which the openings 230b in a central portion of the device are
filled with a beneficial agent and the openings 230a at the edges
of the device remain empty. The device of FIG. 2 is referred to as
having "cool ends."
[0056] In addition to use in reducing edge effect restenosis, the
expandable medical device 200 of FIG. 2 may be used in conjunction
with the expandable medical device 10 of FIG. 1 or another drug
delivery stent when an initial stenting procedure has to be
supplemented with an additional stent. For example, in some cases
the device 10 of FIG. 1 with "hot ends" or a device with uniform
distribution of drug may be implanted improperly. If the physician
determines that the device does not cover a sufficient portion of
the lumen a supplemental device may be added at one end of the
existing device and slightly overlapping the existing device. When
the supplemental device is implanted, the device 200 of FIG. 2 is
used so that the "cool ends" of the medical device 200 prevent
double-dosing of the beneficial agent at the overlapping portions
of the devices 10, 200.
[0057] FIG. 3 illustrates a further alternate exemplary embodiment
of the invention in which different beneficial agents are
positioned in different holes of an expandable medical device 300.
A first beneficial agent is provided in holes 330a at the ends of
the device and a second beneficial agent is provided in holes 330b
at a central portion of the device. The beneficial agent may
contain different drugs, the same drugs in different
concentrations, or different variations of the same drug. The
exemplary embodiment of FIG. 3 may be used to provide an expandable
medical device 300 with either "hot ends" or "cool ends."
[0058] Preferably, each end portion of the device 300 which
includes the holes 330a comprising the first beneficial agent
extends at least one hole and up to about fifteen holes from the
edge. This distance corresponds to about 0.005 to about 0.1 inches
from the edge of an unexpanded device. The distance from the edge
of the device 300 which includes the first beneficial agent is
preferably about one section, where a section is defined between
the bridging elements.
[0059] Different beneficial agents comprising different drugs may
be disposed in different openings in the stent. This allows the
delivery of two or more beneficial agents from a single stent in
any desired delivery pattern. Alternately, different beneficial
agents comprising the same drug in different concentrations may be
disposed in different openings. This allows the drug to be
uniformly distributed to the tissue with a non-uniform device
structure.
[0060] The two or more different beneficial agents provided in the
devices described herein may comprise (1) different drugs; (2)
different concentrations of the same drug; (3) the same drug with
different release kinetics, i.e., different matrix erosion rates;
or (4) different forms of the same drug. Examples of different
beneficial agents comprising the same drug with different release
kinetics may use different carriers to achieve the elution profiles
of different shapes. Some examples of different forms of the same
drug include forms of a drug having varying hydrophilicity or
lipophilicity.
[0061] In one example of the device 300 of FIG. 3, the holes 330a
at the ends of the device are loaded with a first beneficial agent
comprising a drug with a high lipophilicity while holes 330b at a
central portion of the device are loaded with a second beneficial
agent comprising the drug with a lower lipophilicity. The first
high lipophilicity beneficial agent at the "hot ends" will diffuse
more readily into the surrounding tissue reducing the edge effect
restenosis.
[0062] The device 300 may have an abrupt transition line at which
the beneficial agent changes from a first agent to a second agent.
For example, all openings within 0.05 inches of the end of the
device may comprise the first agent while the remaining openings
comprise the second agent. Alternately, the device may have a
gradual transition between the first agent and the second agent.
For example, a concentration of the drug in the openings may
progressively increase (or decrease) toward the ends of the device.
In another example, an amount of a first drug in the openings
increases while an amount of a second drug in the openings
decreases moving toward the ends of the device.
[0063] FIG. 4 illustrates a further alternate exemplary embodiment
of an expandable medical device 400 in which different beneficial
agents are positioned in different openings 430a, 430b in the
device in an alternating or interspersed manner. In this manner,
multiple beneficial agents may be delivered to tissue over the
entire area or a portion of the area supported by the device. This
exemplary embodiment will be useful for delivery of multiple
beneficial agents where combination of the multiple agents into a
single composition for loading in the device is not possible due to
interactions or stability problems between the beneficial
agents.
[0064] In addition to the use of different beneficial agents in
different openings to achieve different drug concentrations at
different defined areas of tissue, the loading of different
beneficial agents in different openings may be used to provide a
more even spatial distribution of the beneficial agent delivered in
instances where the expandable medical device has a non-uniform
distribution of openings in the expanded configuration.
[0065] The use of different drugs in different openings in an
interspersed or alternating manner allows the delivery of two
different drugs which may not be deliverable if combined within the
same polymer/drug matrix composition. For example, the drugs
themselves may interact in an undesirable way. Alternately, the two
drugs may not be compatible with the same polymers for formation of
the matrix or with the same solvents for delivery of the
polymer/drug matrix into the openings.
[0066] Further, the exemplary embodiment of FIG. 4 having different
drugs in different openings in an interspersed arrangement provide
the ability to deliver different drugs with very different desired
release kinetics from the same medical device or stent and to
optimize the release kinetic depending on the mechanism of action
and properties of the individual agents. For example, the water
solubility of an agent greatly affects the release of the agent
from a polymer or other matrix. A highly water soluble compound
will generally be delivered very quickly from a polymer matrix,
whereas, a lipophilic agent will be delivered over a longer time
period from the same matrix. Thus, if a hydrophilic agent and a
lipophilic agent are to be delivered as a dual drug combination
from a medical device, it is difficult to achieve a desired release
profile for these two agents delivered from the same polymer
matrix.
[0067] The system of FIG. 4 allows the delivery of a hydrophilic
and a lipophilic drug easily from the same stent. Further, the
system of FIG. 4 allows the delivery two agents at two different
release kinetics and/or administration periods. Each of the initial
release in the first twenty-four hours, the release rate following
the first twenty-four hours, the total administration period and
any other characteristics of the release of the two drugs may be
independently controlled. For example the release rate of the first
beneficial agent can be arranged to be delivered with at least
forty percent (preferably at least fifty percent) of the drug
delivered in the first twenty-four hours and the second beneficial
agent may be arranged to be delivered with less than twenty percent
(preferably less than ten percent) of the drug delivered in the
first twenty-four hours. The administration period of the first
beneficial agent may be about three weeks or less (preferably two
weeks or less) and the administration period of the second
beneficial agent may be about four weeks or more.
[0068] Restenosis or the recurrence of occlusion post-intervention,
involves a combination or series of biological processes. These
processes include the activation of platelets and macrophages.
Cytokines and growth factors contribute to smooth muscle cell
proliferation and upregulation of genes and metalloproteinases lead
to cell growth, remodeling of extracellular matrix, and smooth
muscle cell migration. A drug therapy which addresses a plurality
of these processes by a combination of drugs may be the most
successfully antirestenotic therapy. The present invention provides
a means to achieve such a successful combination drug therapy.
[0069] The examples discussed below illustrate some of the combined
drug systems which benefit from the ability to release different
drugs in different holes or openings. One example of a beneficial
system for delivering two drugs from interspersed or alternating
holes is the delivery of an anti-inflammatory agent or an
immunosuppressant agent in combination with an antiproliferative
agent or an anti-migratory agent. Other combinations of these
agents may also be used to target multiple biological processes
involved in restenosis. The anti-inflammatory agent mitigates the
initial inflammatory response of the vessel to the angioplasty and
stenting and is delivered at a high rate initially followed by a
slower delivery over a time period of about two weeks to match the
peak in the development of macrophages which stimulate the
inflammatory response. The antiproliferative agent is delivered at
a relatively even rate over a longer time period to reduce smooth
muscle cell migration and proliferation.
[0070] In addition to the examples that are be given below, the
following chart illustrates some of the useful two drug combination
therapies which may be achieved by placing the drugs into different
openings in the medical device.
TABLE-US-00001 PTX Epothilone Imatinibmesylate Rapamycin PKC-
ApoA-I 2-Cda D Gleevec analog Pimecrolimus 412 Dexamethasone
Farglitazar Insulin VIP milano PTX x x x x x x x x 2-CdA x x x x x
s Epothilone D x x x x x x Imatinib x x x x mesylate Gleevec
Rapamycin x x x x x analog Pimecrolimus x x x x x PKC-412 x x x x
Dexamethasone x x Farglitazar x x Insulin x VIP x ApoA-I milano
[0071] The placement of the drugs in different openings allows the
release kinetics to be tailored to the particular agent regardless
of the hydrophobilicity or lipophobicity of the drug. Examples of
some arrangements for delivery of a lipophilic drug at a
substantially constant or linear release rate are described in WO
04/110302 published on Dec. 23, 2004, which is incorporated herein
by reference in its entirety. Examples of some of the arrangements
for delivery of hydrophilic drug are described in WO 04/043510,
published on May 27, 2004 which is incorporated herein by reference
in its entirety. The hydrophilic drugs listed above include CdA,
Gleevec, VIP, insulin, and ApoA-1 milano. The lipophilic drugs
listed above include paclitaxel, Epothilone D, rapamycin,
pimecrolimus, PKC-412 and Dexamethazone. Farglitazar is partly
liphophillic and partly hydrophilic.
[0072] In addition to the delivery of multiple of drugs to address
different biological processes involved in restenosis, the present
invention may deliver two different drugs for treatment of
different diseases from the same stent. For example, a stent may
deliver an anti-proliferative, such as paclitaxel or a limus drug
from one set of openings for treatment of restenosis while
delivering a myocardial preservative drug, such as insulin, from
other openings for the treatment of acute myocardial
infarction.
[0073] In many of the known expandable devices and for the device
illustrated in FIG. 5 the coverage of the device 500 is greater at
the cylindrical tube portions 512 of the device than at the
bridging elements 514. Coverage is defined as the ratio of the
device surface area to the area of the lumen in which the device is
deployed. When a device with varying coverage is used to deliver a
beneficial agent contained in openings in the device, the
beneficial agent concentration delivered to the tissue adjacent the
cylindrical tube portions 512 is greater that the beneficial agent
delivered to the tissue adjacent the bridging elements 514. In
order to address this longitudinal variation in device structure
and other variations in device coverage which lead to uneven
beneficial agent delivery concentrations, the concentration of the
beneficial agent may be varied in the openings at portions of the
device to achieve a more even distribution of the beneficial agent
throughout the tissue. In the case of the exemplary embodiment
illustrated in FIG. 5, the openings 530a in the tube portions 512
include a beneficial agent with a lower drug concentration than the
openings 530b in the bridging elements 514. The uniformity of agent
delivery may be achieved in a variety of manners including varying
the drug concentration, the opening diameter or shape, the amount
of agent in the opening (i.e., the percentage of the opening
filed), the matrix material, or the form of the drug.
[0074] Another example of an application for the use of different
beneficial agents in different openings is in an expandable medical
device 600, as illustrated in FIG. 6, configured for use at a
bifurcation in a vessel. Bifurcation devices include a side hole
610 which is positioned to allow blood flow through a side branch
of a vessel. One example of a bifurcation device is described in
U.S. Pat. No. 6,293,967 which is incorporated herein by reference
in its entirety. The bifurcation device 600 includes the side hole
feature 610 interrupting the regular pattern of beams which form a
remainder of the device. Since an area around a bifurcation is a
particularly problematic area for restenosis, a concentration of an
antiproliferative drug may be increased in openings 830a at an area
surrounding the side hole 610 of the device 600 to deliver
increased concentrations of the drug where needed. The remaining
openings 630b in an area away from the side opening contain a
beneficial agent with a lower concentration of the
antiproliferative. The increased antiproliferative delivered to the
region surrounding the bifurcation hole may be provided by a
different beneficial agent containing a different drug or a
different beneficial agent containing a higher concentration of the
same drug.
[0075] In addition to the delivery of different beneficial agents
to the mural or abluminal side of the expandable medical device for
treatment of the vessel wall, beneficial agents may be delivered to
the luminal side of the expandable medical device to prevent or
reduce thrombosis. Drugs which are delivered into the blood stream
from the luminal side of the device may be located at a proximal
end of the device or a distal end of the device.
[0076] The methods for loading different beneficial agents into
different openings in an expandable medical device may include
known techniques such as dipping and coating and also known
piezoelectric micro-jetting techniques. Micro-injection devices may
be computer controlled to deliver precise amounts of two or more
liquid beneficial agents to precise locations on the expandable
medical device in a known manner. For example, a dual agent jetting
device may deliver two agents simultaneously or sequentially into
the openings. When the beneficial agents are loaded into through
openings in the expandable medical device, a luminal side of the
through openings may be blocked during loading by a resilient
mandrel allowing the beneficial agents to be delivered in liquid
form, such as with a solvent. The beneficial agents may also be
loaded by manual injection devices.
EXAMPLE 1
[0077] FIG. 7 illustrates a dual drug stent 700 having an
anti-inflammatory agent and an antiproliferative agent delivered
from different holes in the stent to provide independent release
kinetics of the two drugs which are specifically programmed to
match the biological processes of restenosis. According to this
example, the dual drug stent includes an anti-inflammatory agent
pimecrolimus in a first set of openings 710 in combination with the
antiproliferative agent paclitaxel in a second set of openings 720.
Each agent is provided in a matrix material within the holes of the
stent in a specific inlay arrangement designed to achieve the
release kinetics illustrated in FIG. 8. Each of the drugs are
delivered primarily murally for treatment of restenosis.
[0078] As illustrated in FIG. 7, pimecrolimus is provided in the
stent for directional delivery to the mural side of the stent by
the use of a barrier 712 at the luminal side of the hole. The
barrier 712 is formed by a biodegradable polymer. The pimecrolimus
is loaded within the holes in a manner which creates a release
kinetics having dual phases. A first phase of the release of
pimecrolimus is provided by a murally located region 716 of the
matrix which has a fast release formulation including pimecrolimus
and biodegradable polymer (PLGA) with a high percentage of drug,
such as about ninety percent drug to about ten percent polymer. A
second phase of the release is provided by a central region 714 of
the matrix with pimecrolimus and biodegradable polymer (PLGA) in a
ratio of about fifty percent drug to fifty percent polymer. As may
be seen on the graph of FIG. 8, the first phase of the pimecrolimus
release delivers about fifty percent of the loaded drug in about
the first twenty-four hours. The second phase of the release
delivers the remaining fifty percent over about two weeks. This
release is specifically programmed to match the progression of the
inflammatory process following angioplasty and stenting. In
addition to or as an alternative to changing the drug concentration
between the two regions to achieve the two phase release, different
polymers or different comonomer ratios of the same polymer may be
used in two drug different regions to achieve the two different
release rates.
[0079] The paclitaxel is loaded within the openings 720 in a manner
which creates a release kinetic having a substantially linear
release after the first approximately twenty-four hours, as
illustrated in FIG. 8. The paclitaxel openings 720 are loaded with
three regions including a base region 722 of primarily polymer with
minimal drug at a luminal side of the hole, a central region 724
with paclitaxel and polymer (PLGA) provided in a concentration
gradient, and a cap region 726 with primarily polymer which
controls release of the paclitaxel. The paclitaxel is released with
an initial release in the first day of about five to about fifteen
percent of the total drug load followed by a substantially linear
release for about twenty to ninety days. Additional examples of
arrangements for paclitaxel in the holes with a concentration
gradient are described in WO 04/110302 set forth above.
[0080] FIG. 7 illustrates the drug, barrier, and cap regions as
distinct regions within the openings for ease of illustration. It
should be understood that these regions indistinct and formed by a
blending of the different areas. Thus, although the barrier layers
are primarily polymer without drug, depending on the manufacturing
processes employed, some small amount of drug of the subsequent
region can be incorporation into the barrier region.
[0081] The amount of the drugs delivered varies depending on the
size of the stent. For a three mm by six mm stent the amount of
pimecrolimus is about fifty to about three hundred micrograms
preferably about one hundred to about two hundred fifty micrograms.
The amount of paclitaxel delivered from this stent is about five to
about fifty micrograms preferably about ten to about thirty
micrograms. In one example, about two hundred micrograms of
pimecrolimus and about twenty micrograms of paclitaxel are
delivered. The drugs may be located in alternating holes in the
stent. However, in view of the large difference in the doses to be
delivered between the two drugs, it may be desirable to place the
paclitaxel in every third of fourth hole in the stent.
Alternatively, the holes for delivery of the low dose drug
(paclitaxel) may be made smaller than the holes for the high
dose.
[0082] The polymer/drug inlays are formed by computer controlled
piezoelectric injection techniques as described in WO 04/026182
published on Apr. 1, 2004, which is incorporated herein by
reference in its entirety. The inlays of the first agent may be
formed first followed by the inlays of the second agent using the
piezoelectric injector. Alternately, the system of WO 04/02182 may
be equipped with dual piezoelectric dispensers for dispensing the
two agents at the same time.
EXAMPLE 2
[0083] According to this example, the dual drug stent includes the
Gleevec in the first set of openings 710 in combination with the
antiproliferative agent paclitaxel in the second set of openings
720. Each agent is provided in a matrix material within the holes
of the stent in a specific inlay arrangement designed to achieve
the release kinetics illustrated in FIG. 8.
[0084] The Gleevec is delivered with a two phase release including
a high initial release in the first day and then a slow release for
one to two weeks. The first phase of the Gleevec release delivers
about fifty percent of the loaded drug in about the first
twenty-four hours. The second phase of the release delivers the
remaining fifty percent over about one-two weeks. The paclitaxel is
loaded within the openings 720 in a manner which creates a release
kinetics having a substantially linear release after the first
approximately twenty-four hours, as illustrated in FIG. 8 and as
described above in Example 1.
[0085] The amount of the drugs delivered varies depending on the
size of the stent. For a three mm by six mm stent the amount of
Gleevec is about two hundred to about five hundred micrograms,
preferably about three hundred to about four hundred micrograms.
The amount of paclitaxel delivered from this stent is about five to
about fifty micrograms, preferably about ten to about thirty
micrograms. As in Example 1, the drugs may be located in
alternating holes in the stent or interspersed in a non-alternating
manner. The polymer/drug inlays are formed in the manner described
in Example 1.
EXAMPLE 3
[0086] According to this example, the dual drug stent includes the
PKC-412 (a cell growth regulator) in the first set of openings in
combination with the antiproliferative agent paclitaxel in the
second set of openings. Each agent is provided in a matrix material
within the holes of the stent in a specific inlay arrangement
designed to achieve the release kinetics discussed below.
[0087] The PKC-412 is delivered at a substantially constant release
rate after the first approximately twenty-four hours, with the
release over a period of about four to sixteen weeks, preferably
about six to twelve weeks. The paclitaxel is loaded within the
openings in a manner which creates a release kinetic having a
substantially linear release after the first approximately
twenty-four hours, with the release over a period of about four to
sixteen weeks, preferably about six to twelve weeks.
[0088] The amount of the drugs delivered varies depending on the
size of the stent. For a three mm by six mm stent the amount of
PKC-412 is about one hundred to about four hundred micrograms,
preferably about one hundred fifty to about two hundred fifty
micrograms. The amount of paclitaxel delivered from this stent is
about five to about fifty micrograms, preferably about ten to about
thirty micrograms. As in Example 1, the drugs may be located in
alternating holes in the stent or interspersed in a non-alternating
manner. The polymer/drug inlays are formed in the manner described
in Example 1.
Therapeutic Agents
[0089] The present invention relates to the delivery of
anti-restenotic agents including paclitaxel, rapamycin, cladribine
(CdA), and their derivatives, as well as other cytotoxic or
cytostatic agents and microtubule stabilizing agents. Although
anti-restenotic agents have been primarily described herein, the
present invention may also be used to deliver other agents alone or
in combination with anti-restenotic agents. Some of the therapeutic
agents for use with the present invention which may be transmitted
primarily luminally, primarily murally, or both and may be
delivered alone or in combination include, but are not limited to,
antiproliferatives, antithrombins, immunosuppressants including
sirolimus, antilipid agents, anti-inflammatory agents,
antineoplastics, antiplatelets, angiogenic agents, anti-angiogenic
agents, vitamins, antimitotics, metalloproteinase inhibitors, NO
donors, estradiols, anti-sclerosing agents, and vasoactive agents,
endothelial growth factors, estrogen, beta blockers, AZ blockers,
hormones, statins, insulin growth factors, antioxidants, membrane
stabilizing agents, calcium antagonists, retenoid, bivalirudin,
phenoxodiol, etoposide, ticlopidine, dipyridamole, and trapidil
alone or in combinations with any therapeutic agent mentioned
herein. Therapeutic agents also include peptides, lipoproteins,
polypeptides, polynucleotides encoding polypeptides, lipids,
protein-drugs, protein conjugate drugs, enzymes, oligonucleotides
and their derivatives, ribozymes, other genetic material, cells,
antisense, oligonucleotides, monoclonal antibodies, platelets,
prions, viruses, bacteria, and eukaryotic cells such as endothelial
cells, stem cells, ACE inhibitors, monocyte/macrophages or vascular
smooth muscle cells to name but a few examples. The therapeutic
agent may also be a pro-drug, which metabolizes into the desired
drug when administered to a host. In addition, therapeutic agents
may be pre-formulated as microcapsules, microspheres, microbubbles,
liposomes, niosomes, emulsions, dispersions or the like before they
are incorporated into the therapeutic layer. Therapeutic agents may
also be radioactive isotopes or agents activated by some other form
of energy such as light or ultrasonic energy, or by other
circulating molecules that can be systemically administered.
Therapeutic agents may perform multiple functions including
modulating angiogenesis, restenosis, cell proliferation,
thrombosis, platelet aggregation, clotting, and vasodilation.
[0090] Anti-inflammatories include but are not limited to
non-steroidal anti-inflammatories (NSAID), such as aryl acetic acid
derivatives, e.g., Diclofenac; aryl propionic acid derivatives,
e.g., Naproxen; and salicylic acid derivatives, e.g., Diflunisal.
Anti-inflammatories also include glucocoriticoids (steroids) such
as dexamethasone, aspirin, prednisolone, and triamcinolone,
pirfenidone, meclofenamic acid, tranilast, and nonsteroidal
anti-inflammatories. Anti-inflammatories may be used in combination
with antiproliferatives to mitigate the reaction of the tissue to
the antiproliferative.
[0091] The agents may also include anti-lymphocytes;
anti-macrophage substances; immunomodulatory agents; cyclooxygenase
inhibitors; anti-oxidants; cholesterol-lowering drugs; statins and
angiotens in converting enzyme (ACE); fibrinolytics; inhibitors of
the intrinsic coagulation cascade; antihyperlipoproteinemics; and
anti-platelet agents; anti-metabolites, such as 2-chlorodeoxy
adenosine (2-CdA or cladribine); immuno-suppressants including
sirolimus, everolimus, tacrolimus, etoposide, and mitoxantrone;
anti-leukocytes such as 2-CdA, IL-1 inhibitors, anti-CD116/CD18
monoclonal antibodies, monoclonal antibodies to VCAM or ICAM, zinc
protoporphyrin; anti-macrophage substances such as drugs that
elevate NO; cell sensitizers to insulin including glitazones; high
density lipoproteins (HDL) and derivatives; and synthetic facsimile
of HDL, such as lipator, lovestatin, pranastatin, atorvastatin,
simvastatin, and statin derivatives; vasodilators, such as
adenosine, and dipyridamole; nitric oxide donors; prostaglandins
and their derivatives; anti-TNF compounds; hypertension drugs
including Beta blockers, ACE inhibitors, and calcium channel
blockers; vasoactive substances including vasoactive intestinal
polypeptides (VIP); insulin; cell sensitizers to insulin including
glitazones, P par agonists, and metformin; protein kinases;
antisense oligonucleotides including resten-NG; antiplatelet agents
including tirofiban, eptifibatide, and abciximab; cardio
protectants including, VIP, pituitary adenylate cyclase-activating
peptide (PACAP), apoA-I milano, amlodipine, nicorandil,
cilostaxone, and thienopyridine; cyclooxygenase inhibitors
including COX-1 and COX-2 inhibitors; and petidose inhibitors which
increase glycolitic metabolism including omnipatrilat. Other drugs
which may be used to treat inflammation include lipid lowering
agents, estrogen and progestin, endothelin receptor agonists and
interleukin-6 antagonists, and Adiponectin. Therapeutic agents may
also include phosphodiesterase inhibitors (PDEi), such as
cilastazol and adenosine receptor agonists, preferably A.sub.2A
receptor, agonists such as regadenoson.
[0092] Agents may also be delivered using a gene therapy-based
approach in combination with an expandable medical device. Gene
therapy refers to the delivery of exogenous genes to a cell or
tissue, thereby causing target cells to express the exogenous gene
product. Genes are typically delivered by either mechanical or
vector-mediated methods.
[0093] Some of the agents described herein may be combined with
additives which preserve their activity. For example additives
including surfactants, antacids, antioxidants, and detergents may
be used to minimize denaturation and aggregation of a protein drug.
Anionic, cationic, or nonionic surfactants may be used. Examples of
nonionic excipients include but are not limited to sugars including
sorbitol, sucrose, trehalose; dextrans including dextran, carboxy
methyl (CM) dextran, diethylamino ethyl (DEAE) dextran; sugar
derivatives including D-glucosaminic acid, and D-glucose diethyl
mercaptal; synthetic polyethers including polyethylene glycol (PEO)
and polyvinyl pyrrolidone (PVP); carboxylic acids including
D-lactic acid, glycolic acid, and propionic acid; surfactants with
affinity for hydrophobic interfaces including
n-dodecyl-.beta.-D-maltoside, n-octyl-.beta.-D-glucoside, PEO-fatty
acid esters (e.g. stearate (myrj 59) or oleate), PEO-sorbitan-fatty
acid esters (e.g. Tween 80, PEO-20 sorbitan monooleate),
sorbitan-fatty acid esters (e.g. SPAN 60, sorbitan monostearate),
PEO-glyceryl-fatty acid esters; glyceryl fatty acid esters (e.g.
glyceryl monostearate), PEO-hydrocarbon-ethers (e.g. PEO-10 oleyl
ether; triton X-100; and Lubrol. Examples of ionic detergents
include but are not limited to fatty acid salts including calcium
stearate, magnesium stearate, and zinc stearate; phospholipids
including lecithin and phosphatidyl choline; (PC) CM-PEG; cholic
acid; sodium dodecyl sulfate (SDS); docusate (AOT); and taumocholic
acid.
[0094] In accordance with another exemplary embodiment, a stent or
intraluminal scaffold as described herein, may be coated with an
anti-thrombotic agent in addition to one or more therapeutic agents
deposited in the holes or openings. In one exemplary embodiment,
the stent may be fabricated with the openings therein and prior to
the addition or deposition of other therapeutic agents into the
openings, an anti-thrombotic agent, with or without a carrier
vehicle (polymer or polymeric matrix) may be affixed to the stent
or a portion thereof. In this exemplary embodiment, the luminal and
abluminal surfaces of the stent may be coated with the
anti-thrombotic agent or coating, as well as the surfaces of the
walls of the openings. In an alternative exemplary embodiment, a
stent may first be coated with an anti-thrombotic agent or coating
and then the openings may be fabricated. In this exemplary
embodiment, only the luminal and abluminal surfaces would have the
anti-thrombotic agent or coating and not the walls of the openings.
In each of these embodiments any number of anti-thrombotic agents
may be affixed to all or portions of the stents. In addition, any
number of known techniques may be utilized to affix the
anti-thrombotic agent to the stent such as that utilized with the
HEPACOAT.TM. on the Bx Velocity.RTM. Coronary Stent from Cordis
Corporation. Alternatively, the stents may be manufactured with a
rough surface texture or have a micro-texture to enhance cell
attachment and endothelialization, independently of or in addition
to the anti-thrombotic coating. In addition, any number of
therapeutic agents may be deposited into the openings and different
agents may be utilized in different regions of the stent.
[0095] As described above, it is important to note that any number
of drugs and or agents may be utilized in accordance with the
present invention including: antiproliferative/antimitotic agents
including natural products such as vinca alkaloids (i.e.
vinblastine, vincristine, and vinorelbine), paclitaxel,
epidipodophyllotoxins (i.e. etoposide, teniposide), antibiotics
(dactinomycin (actinomycin D) daunorubicin, doxorubicin and
idarubicin), anthracyclines, mitoxantrone, bleomycins, plicamycin
(mithramycin) and mitomycin, enzymes (L-asparaginase which
systemically metabolizes L-asparagine and deprives cells which do
not have the capacity to synthesize their own asparagine);
antiplatelet agents such as G(GP)II.sub.bIII.sub.a inhibitors and
vitronectin receptor antagonists; antiproliferative/antimitotic
alkylating agents such as nitrogen mustards (mechlorethamine,
cyclophosphamide and analogs, melphalan, chlorambucil),
ethylenimines and methylmelamines (hexamethylmelamine and
thiotepa), alkyl sulfonates-busulfan, nirtosoureas (carmustine
(BCNU) and analogs, streptozocin), trazenes--dacarbazinine (DTIC);
antiproliferative/antimitotic antimetabolites such as folic acid
analogs (methotrexate), pyrimidine analogs (fluorouracil,
floxuridine, and cytarabine), purine analogs and related inhibitors
(mercaptopurine, thioguanine, pentostatin and
2-chlorodeoxyadenosine {cladribine}); platinum coordination
complexes (cisplatin, carboplatin), procarbazine, hydroxyurea,
mitotane, aminoglutethimide; hormones (i.e. estrogen);
anticoagulants (heparin, synthetic heparin salts and other
inhibitors of thrombin); fibrinolytic agents (such as tissue
plasminogen activator, streptokinase and urokinase), aspirin,
dipyridamole, ticlopidine, clopidogrel, abciximab; antimigratory;
antisecretory (breveldin); antiinflammatory: such as adrenocortical
steroids (cortisol, cortisone, fludrocortisone, prednisone,
prednisolone, 6.alpha.-methylprednisolone, triamcinolone,
betamethasone, and dexamethasone), non-steroidal agents (salicylic
acid derivatives i.e. aspirin; para-aminophenol derivatives i.e.
acetominophen; indole and indene acetic acids (indomethacin,
sulindac, and etodalac), heteroaryl acetic acids (tolmetin,
diclofenac, and ketorolac), arylpropionic acids (ibuprofen and
derivatives), anthranilic acids (mefenamic acid, and meclofenamic
acid), enolic acids (piroxicam, tenoxicam, phenylbutazone, and
oxyphenthatrazone), nabumetone, gold compounds (auranofin,
aurothioglucose, gold sodium thiomalate); immunosuppressives:
(cyclosporine, tacrolimus (FK-506), sirolimus (rapamycin),
azathioprine, mycophenolate mofetil); angiogenic agents: vascular
endothelial growth factor (VEGF), fibroblast growth factor (FGF)
platelet derived growth factor (PDGF), erythropoetin; angiotensin
receptor blocker; nitric oxide donors; anti-sense oligionucleotides
and combinations thereof; cell cycle inhibitors, mTOR inhibitors,
and growth factor signal transduction kinase inhibitors.
[0096] Referring now to FIGS. 9A, 9B and 9C, there is illustrated a
diagrammatic representation of a portion of a stent.
[0097] As illustrated in FIG. 9A the stent 900 comprises a
plurality of substantially circular openings 902. In this exemplary
embodiment, the plurality of substantially circular openings 902
extend through the wall of the stent 900. In other words, the
plurality of substantially circular openings 902 extend from the
abluminal surface of the stent 904 to the abluminal surface of the
stent 906, wherein the wall thickness is defined as the distance
between the luminal and abluminal surfaces. In other embodiments;
however, the openings need not extend through the wall of the stent
900. For example, the openings or reservoirs may extend partially
from either the luminal or abluminal surfaces or both. The stent
900 in FIG. 9A has untreated surfaces 904 and 906 and empty
openings 902.
[0098] In FIG. 9B, at least one surface has been coated with a
therapeutic agent 908. The therapeutic agent preferably comprises
an anti-thrombotic agent such as heparin; however, any
anti-thrombotic agent may be utilized. The anti-thrombotic agent
may be affixed utilizing any technique as briefly described above.
In this exemplary embodiment, both the abluminal and luminal
surfaces have an anti-thrombotic agent affixed thereto. In
addition, as there is nothing in the plurality of substantially
circular openings 902 at this juncture, the walls of the openings
902 may also have some anti-thrombotic agent affixed thereto. The
amount of anti-thrombotic agent affixed to the walls of the
openings 910 depends on how the agent is affixed. For example, if
the agent is affixed by dip coating, the walls of the openings will
have more agent affixed thereto than if the agent is affixed
utilizing a spray coating technique. As described herein, in this
exemplary embodiment, all exposed surfaces have a substantial
anti-thrombotic coating affixed thereto; however, in alternate
exemplary embodiments, only specific surfaces may have an
anti-thrombotic affixed thereto. For example, in one exemplary
embodiment, only the surface in contact with the blood may be
treated with the anti-thromobotic agent. In yet another alternate
exemplary embodiment, one or both surfaces may be coated with the
anti-thrombotic agent while the walls of the openings are not. This
may be accomplished in a number of ways including plugging the
openings prior to coating or creating the openings after the
anti-thrombotic agent is affixed.
[0099] FIG. 9C illustrates a completed stent in accordance with
this exemplary embodiment. As illustrated in this figure, the
plurality of substantially circular openings 902 have been filled
with one or more therapeutic agents for treating vascular diseases
such as restenosis and inflammation or any other dieses as
described herein. Each opening 902 may be filled with the same
therapeutic agent or different agents as described in detail above.
As illustrated in the figure, these different agents 912, 914 and
916 are used in a particular pattern; however, as detailed above,
any combination is possible as well as utilizing a singe agent with
different concentrations. The drugs, such as a rapamycin, may be
deposited in the openings 902 in any suitable manner. Techniques
for depositing the agent include micro-pippetting and/or ink-jet
filling methods. In one exemplary embodiment, the drug filling may
be done so that the drug and/or drug/polymer matrix in the opening
will be below the level of the stent surfaces so that there is no
contact with the surrounding tissue. Alternately, the openings may
be filled so that the drug and/or drug/polymer matrix may contact
the surrounding tissue. In addition, the total dose of each of the
drugs, if multiple drugs are utilized, may be designed with maximal
flexibility. Additionally, the release rate of each of the drugs
may be controlled individually. For example, the openings near the
ends may contain more drugs to treat edge restenosis.
[0100] In accordance with this exemplary embodiment, the hole or
openings may be configured not only for the most efficacious drug
therapy, but also for creating a physical separation between
different drugs. This physical separation may aid in preventing the
agents from interacting.
[0101] As used herein, rapamycin includes rapamycin and all
analogs, derivatives and conjugates that bind to FKBP12, and other
immunophilins and possesses the same pharmacologic properties as
rapamycin including inhibition of TOR. In addition, all drugs and
agents described herein in their analogs, derivatives and
conjugates.
[0102] As described herein, a stent having through-holes, holes,
reservoirs or openings therein may be coated with an
anti-thrombotic agent and/or drug or combination of drugs such as
those described herein, and the openings filled with one or more
therapeutic agents alone or in combination with one or more
polymers. Essentially, the stent may be fabricated with the
openings therein and prior to the addition or deposition of
therapeutic agents alone or in combination with one or more
polymers into the openings, an anti-thrombotic agent, with or
without a carrier vehicle, may be affixed to the stent or a portion
thereof. In the exemplary embodiment as described herein, the
luminal and abluminal surfaces of the stent as well as the surfaces
of the walls of the openings may be coated with the
anti-thromobotic agent. In this exemplary embodiment, the
anti-thrombotic agent comprises heparin or its various derivatives
such as low molecular-weight heparin (LMWH), although any number of
suitable anti-thrombotic agents may be utilized. Heparin and/or
LMWH have very high negative charges.
[0103] The entire surface of the stent described herein, including
the interior surfaces of the through-holes or openings that become
reservoirs for the therapeutic agent and/or combination polymers
and therapeutic agent, is first given a covalently bonded heparin
coating. The heparin coating itself is bonded to the metal surface
of the stent by its own primer comprising alternating layers of
poly(ethyleneimine), a strongly cationic polymer known by the
abbreviation PEI, and dextran sulfate, a polymeric anion. The
application of this type of primer is known in the art and is set
forth in a number of patents, including U.S. Pat. Nos. 5,213,898,
5,049,403, 6,461,665 and 6,767,405. More specifically, the heparin
is covalently bonded to the primer, including the PEI-dextran
sulfate layers, which is in turn bonded to the metal surface. Once
all surfaces are coated with the heparin mixture, each of the holes
or reservoirs are filled utilizing one of the processes described
herein.
[0104] In accordance with another exemplary embodiment, the present
invention is directed to primer compositions and configurations for
improving the adhesion of a drug delivery matrix, e.g. therapeutic
agent and polymer combination, to a heparin coated surface of a
medical device, for example, a stent. The present invention is
particularly advantageous where the heparin coating is covalently
bonded to a metallic or polymer surface of the medical device. In
the present invention, the primer preferably comprises a high
molecular weight component or a low molecular weight component, and
the drug delivery matrix comprises a drug and/or other beneficial
agent and an excipient, preferably a polymeric excipient. In
addition, the primer may also preferably comprise a material having
an opposite electrical charge and similar charge density to that of
the underlying layer, for example, heparin.
[0105] The concept of a primer on top of a heparin layer or coating
to increase the bonding of a heparin coated surface to any other
matrix or coating is unique given that typically the heparin
surface is utilized to confer anti-thromobotic properties and hence
will not be covered in practical uses. In the present invention, it
is only the interior wall surfaces of the holes or openings in the
stent that hold the drug-polymer reservoirs that will be covered
with the primer of the present invention, thus increasing the
adhesion between the two layers and limiting the potential loss of
the drug-polymer matrix without substantially affecting the heparin
surface outside the reservoirs. It is important to note that the
heparin blocking primers in accordance with the present invention
are biocompatible in their original intended uses.
[0106] The primer of the present invention may be utilized with any
type of stent. In the exemplary embodiment described herein, the
primer is utilized with the stent or stents illustrated in FIGS. 1,
2, 3 and 4.
[0107] In accordance with one exemplary embodiment, the primer
comprises polymer-poly(ethyleneimine) conjugates, for example
polylactic-co-glycolic acid (PLGA) and poly(ethyleneimine) PEI
and/or PLGA-protamine. Poly(ethyleneimine) is a strongly cationic
polymer that binds to certain negatively charged proteins or
polysaccharides. In addition to PEI, the other material useful in
this conjugate is protamine. Protamine is an approved low molecular
weight protein drug that is utilized as an antidote to heparin. It
is sparsely solution in water. In this manner, the primer may
simultaneously interact strongly with both the heparin coating and
the drug containing matrix, thus improving the adhesion between the
two substances. Since heparin is a poly(anionic) species, it is
anticipated that a poly(cationic) species such as protamine would
bond well to the heparin, but would be sufficiently hydrophobic in
the other sections of its structure to allow good bonding of the
PLGA component of the drug polymer matrix in the reservoir.
[0108] The bonding reactions between PLGA and PEI and PEI and
heparin may be ionic-bonding or covalent-bonding reactions. FIG. 10
illustrates an example of covalent-bonding between PLGA and PEI.
More specifically, FIG. 10 illustrates the conjugation reaction
between PLGA with a carboxylic acid end group and low molecular
weight PEI. Alternatively, the primer may comprise a high molecular
weight PEI or a branched PEI. Referring to FIG. 11, there is
illustrated the conjugation reaction between PLGA with a carboxylic
acid end group and a high molecular weight or branched PEI. As
illustrated, the reaction may be configured for a one-to-one ratio
or a conjugate of PLGA-PEI-PLGA for a 2:1 ratio between PLGA and
PEI.
[0109] The Table below illustrates the effectiveness of PEI as a
primer for increasing the adhesion of the drug/polymer complex to
the heparin coated surfaces. The test stents are immersed in a
testing medium comprising a phosphate buffer saline and bovine
serum albumin which simulates physiological fluid conditions. The
drug/polymer complex comprises a rapamycin and PLGA.
TABLE-US-00002 Percent (%) of Empty Reservoirs versus Initial Total
Reservoirs Heparin (after immersion in PBS-BSA) coated Day Day Day
Day Day Day Day Day Day Day Day Day stent Initial 7 14 21 28 30 35
42 49 60 75 90 No primer Avg 0% -- 0% 0.3% 3.1% -- 3.7% 4.1% 4.8%
4.8% 5.1% 5.3% Stdev 0% -- 0% 0.4% 2.1% -- 2.0% 2.0% 1.2% 1.2% 1.3%
1.5% RSD n/a -- n/a 124.9% 68.3% -- 56.0% 48.5% 25.3% 25.3% 25.1%
28.4% Primed with Avg 0.0% 0.0% -- -- -- 0.0% 0.0% 0.25% PEI Stdev
0.0% 0.0% -- -- -- 0.0% 0.0% (linear) in RSD n/a n/a -- -- -- n/a
n/a Water Primed with Avg 0% -- 0% 0% 0% -- 0.1% 0.1% 0.1% 0.1%
0.1% 0.1% 0.5% PEI Stdev 0% -- 0% 0% 0% -- 0% 0% 0% 0% 0% 0%
(linear) in RSD n/a -- n/a n/a n/a -- n/a n/a n/a n/a n/a n/a Water
Primed with Avg 0% -- 0% 0% 0% -- 0.1% 0.1% 0.1% 0.1% 0.1% 0.1%
0.5% PEI Stdev 0% -- 0% 0% 0% -- 0% 0% 0% 0% 0% 0% (linear) in RSD
n/a -- n/a n/a n/a -- n/a n/a n/a n/a n/a n/a DMSO Primed with Avg
0% -- 0% 0% 0% -- 0% 0% 0% 0% 0% 0% 0.5% PEI Stdev 0% -- 0% 0% 0%
-- 0% 0% 0% 0% 0% 0% (branched) RSD n/a -- n/a n/a n/a -- n/a n/a
n/a n/a n/a n/a in Water Primed with Avg 0% -- 0% 0% 0% -- 0% 0% 0%
0% 0% 0% 0.5% PEI Stdev 0% -- 0% 0% 0% -- 0% 0% 0% 0% 0% 0%
(branched) RSD n/a -- n/a n/a n/a -- n/a n/a n/a n/a n/a n/a in
DMSO Primed with Avg 0.0% 0.0% -- -- -- 0.0% 0.0% 0.75% PEI Stdev
0.0% 0.0% -- -- -- 0.0% 0.0% (linear) in RSD n/a n/a -- -- -- n/a
n/a Water
[0110] In an alternate exemplary embodiment, the primer may
comprise low molecular weight complexing cations to heparin,
including benzalkonium chloride and/or oligomeric arginine
peptides, or high molecular weight complexing cations, including
polylysine, poly(arginine), protamine, poly(dimethylaminoethyl)
methacrylate or poly (dimethylaminoethyl) acrylate.
[0111] In accordance with the present invention, the process for
increasing adhesion of the drug complex to the heparin may include
the application of the adhesion promoting primer followed by
polymer/drug fill solution or the application of the adhesion
promoting primer followed by carboxyl-ended PLGA or a blend of
carboxyl-ended and regular PLGA and PLGA/drug fill solution.
[0112] The primer of the present invention will be applied to the
interior, heparin coated walls of the holes or openings in the
stent prior to the openings being filled with a local drug delivery
matrix. In other words, in the finished drug eluting stent, the
primer will occupy a space between the surface of the heparin
coating and the body of the drug delivery matrix and will increase
the adhesion between the heparin coating and the drug delivery
matrix. The enhancement of adhesion is achieved through multiple
factors, including the reduction of osmolarity/water infiltration
of the heparin coating in use after the charge neutralization by a
cationic primer, reduced aqueous solubility of heparin/cationic
primer complex as compared to the heparin surface alone, ionic
bonding, covalent bonding and better physical adhesion between the
primer and the polymer/drug matrix due to surface tension and the
like.
[0113] In another exemplary embodiment of the present invention,
the primer of the present invention will preferably have a portion
of its molecular structure that is positively charged for bonding
to the negatively charged heparin coating, and a portion that is
hydrophobic, hydrophilic, or balanced for bonding to the polymer
component of the drug delivery matrix. This portion of the primer
will vary depending on the nature of the drug delivery matrix. More
specifically, the primer is designed to improve the adhesion of the
drug delivery matrix to the heparin coated openings of the stent so
that none or substantially none of the openings or reservoirs lose
their contents when the stent comes into contact with water based
fluids, such as saline, blood and/or intercellular fluid.
[0114] Although the primer of the present invention has been
described specifically to increasing the adhesion between the
heparin coated interior walls of a stent reservoir and the
drug/polymer mixture filling the reservoir, it may be useful for
the attachment or bonding of any substrate to a portion of a
heparin coated surface. For example, blood contacting plastic
medical devices are often coated with heparin to minimize
thrombosis on the device, but it may be desired to bond later to
that surface. A mixture of the primers of the present invention in
a solvent could be applied to a selected area of the device, the
solvent evaporated to provide a primer coated area on the heparin
surface, then subsequently a new subsystem could be bonded to the
primer covered area.
[0115] The primer material will be advantageously applied as a
solution of the polymeric primer in a solvent such as dimethyl
sulfoxide (DMSO), N-methylpyrrolidone, or water mixtures thereof
and may be introduced into the reservoirs using any of the filling
techniques described in the instant application. Such a primer
solution could then be dried to provide the coating of the primer
layer over the heparin coated surface. Preferably, the application
of the primer layer will require only a single deposition step in
the stent filling process. The selection of a suitable solvent for
the deposition of a cationic primer is determined primarily by its
ability to dissolve a primer and its compatibility with the filling
apparatus and process described herein.
[0116] The present invention may be simply characterized as an
implantable medical device. The medical device comprises an
intraluminal scaffold having a plurality of openings therein, a
first coating comprising a material having a first electric charge
affixed to at least a portion of a surface of the intraluminal
scaffold and a surface of the plurality of openings, a second
coating comprising a material having a second electric charge
affixed to at least a portion of the first coating, the second
electric charge being opposite of the first electric charge, and at
least one therapeutic agent deposited in at least one of the
plurality of openings, wherein the second coating is configured as
an intermediate layer between the first coating and the at least
one therapeutic agent.
[0117] The first coating may comprise any suitable anti-thrombotic
as described herein. For example, a polysaccharide such as heparin
may be utilized. The second coating may comprise a polymeric cation
or a polymeric conjugate having cationic segments as described
herein. Examples of polymeric cations include oligomeric arginine
peptides, polylysine, poly(arginine), protamine,
poly(dimethylaminoethyl) poly(ethyleneimine). Examples of polymeric
cationic conjugates include a first component such as
polylactic-co-glycolic acid and the second component comprises any
of the cations set forth above. The therapeutic agent may comprise
an anti-restenotic, an anti-inflammatory, an anti-thrombotic, an
anti-proliferative, an agent for minimizing damage to infarcted
tissue or any combination thereof.
[0118] In a more general sense, the concept of the present
invention may be expanded to include primers that increase the
bonding strength between hydrophilic and hydrophobic surfaces. For
example, other hydrophilic surfaces of interest are the so called
"lubricious" coatings, such as those utilized in conjunction with
catheters. These hydrophilic surfaces are often also covalently
bonded, but may be just conformal coatings. Examples of chemical
structures that occur in lubricious coatings are those based on
polyvinylpyrrolidone, hydroxyethyl methacrylate, poly(ethylene
oxide) or poly(ethylene glycol) and the like.
[0119] Although shown and described is what is believed to be the
most practical and preferred embodiments, it is apparent that
departures from specific designs and methods described and shown
will suggest themselves to those skilled in the art and may be used
without departing from the spirit and scope of the invention. The
present invention is not restricted to the particular constructions
described and illustrated, but should be constructed to cohere with
all modifications that may fall within the scope of the appended
claims.
* * * * *