U.S. patent number PP33,000 [Application Number 16/501,446] was granted by the patent office on 2021-04-27 for cannabis plant named `og citron`.
This patent grant is currently assigned to Biotech Institute, LLC. The grantee listed for this patent is Biotech Institute, LLC. Invention is credited to Steven Robert Haba, Mark Anthony Lewis.
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United States Patent |
PP33,000 |
Lewis , et al. |
April 27, 2021 |
Cannabis plant named `OG CITRON`
Abstract
Disclosed herein are main terpenes of `OG CITRON`, which are
linalool, limonene, myrcene, beta-caryophyllene, alpha-bisabolol,
alpha-humulene, and beta-pinene. Also, the present invention
provides the estimated concentration of the THC.sub.max,
CBD.sub.max, and CBG.sub.max, about 9.90-12.66%, about 5.50-9.23%,
and about 0.08-0.78%, respectively, at the time of assaying
metabolites from flower samples of `OG CITRON`.
Inventors: |
Lewis; Mark Anthony (Westlake
Village, CA), Haba; Steven Robert (Westlake Village,
CA) |
Applicant: |
Name |
City |
State |
Country |
Type |
Biotech Institute, LLC |
Westlake Village |
CA |
US |
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Assignee: |
Biotech Institute, LLC
(Westlake Village, CA)
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Family
ID: |
66696627 |
Appl.
No.: |
16/501,446 |
Filed: |
April 17, 2019 |
Prior Publication Data
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Document
Identifier |
Publication Date |
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US 20190254213 P1 |
Aug 15, 2019 |
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Related U.S. Patent Documents
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Application
Number |
Filing Date |
Patent Number |
Issue Date |
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16350327 |
Nov 5, 2018 |
PP31917 |
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62596561 |
Dec 8, 2017 |
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Current U.S.
Class: |
PLT/258 |
Current CPC
Class: |
A01H
1/101 (20210101); G06T 7/337 (20170101); G06T
7/75 (20170101); A61K 36/185 (20130101); A01H
5/12 (20130101); A01H 5/02 (20130101); A01H
6/28 (20180501); G06T 2207/20016 (20130101); G06F
3/0481 (20130101); G06T 2207/10032 (20130101) |
Current International
Class: |
A01H
5/02 (20180101); A01H 6/28 (20180101); A61K
36/185 (20060101) |
Field of
Search: |
;PLT/258,263.1 |
References Cited
[Referenced By]
U.S. Patent Documents
Foreign Patent Documents
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WO 2014/145490 |
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Sep 2014 |
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WO |
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WO 2015/065544 |
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May 2015 |
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WO |
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WO 2016/105514 |
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Jun 2016 |
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WO |
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WO 2016/123160 |
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Aug 2016 |
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WO |
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WO 2018/094359 |
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May 2018 |
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WO |
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Other References
Alchimia The effect of cold on Cannabis plants 2017, retrieved on
Mar. 18, 2020, retrieved from the Internet at
https://www.alchimiaweb.com/blogen/the-cold-during-a-cannabis-plant-crop/-
, 13 pp. (Year: 2017). cited by examiner .
Grasscity Forums, "Lemon Crush", 2010, retrieved on May 1, 2019
from https://forum.grasscity.com/threads/ lemon-crush-weed.475046/,
7 pages cited by applicant .
Reddit, "Cake batter?," 2016, retrieved on Jun. 10, 2019, from
https://www.reddit.com/r/CannabisExtracts/comments/4jcow1/cake_batter/,
5 pages. cited by applicant .
Weed Connection, "#Cake Batter #OG," 2016, retrieved on Jun. 11,
2019, from https://weedconnection.com/medz/
link/cakebatterogkush.html, 2 pages. cited by applicant.
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Primary Examiner: Hwu; June
Attorney, Agent or Firm: Cooley LLP
Claims
The invention claimed is:
1. A new and distinct cultivar of Cannabis plant named `OG CITRON`
substantially as shown and described herein.
Description
Latin name of genus and species: Cannabis hybrid.
Variety denomination: `OG CITRON`.
BACKGROUND OF THE INVENTION
The present invention relates to a new and distinct Cannabis
cultivar designated as `OG CITRON`.
This new cultivar is the result of controlled-crosses between
proprietary cultivars made by the inventors. The new cultivar of
`OG CITRON` was asexually reproduced via a `stem cutting` and
`cloning` method by the inventors at Salinas, Calif. Asexual clones
from the original source have been tested in greenhouses,
nurseries, and/or fields. The properties of each cultivar were
found to be transmissible by such asexual reproduction. The
cultivar is stable and reproduces true to type in successive
generations of asexual reproduction.
TAXONOMY AND NOMENCLATURE
Cannabis, more commonly known as marijuana, is a genus of flowering
plants that includes at least three species, Cannabis sativa,
Cannabis indica, and Cannabis ruderalis as determined by plant
phenotypes and secondary metabolite profiles. In practice however,
Cannabis nomenclature is often used incorrectly or interchangeably.
Cannabis literature can be found referring to all Cannabis
varieties as "sativas" or all cannabinoid producing plants as
"indicas". Indeed the promiscuous crosses of indoor Cannabis
breeding programs have made it difficult to distinguish varieties,
with most Cannabis being sold in the United States having features
of both sativa and indica species.
Human cultivation history of Cannabis dates back 8,000 years
(Schultes, R E., 1970, Random thoughts and queries on the botany of
Cannabis. Pages 11-38 in: CRB Joyce, and S H Curry eds., THE BOTANY
AND CHEMISTRY OF CANNABIS. J. & A. Churchill. London, England).
Hemp cloth recovered in Europe dates back 6,000 years (Small, E,
Beckstead, H D, and Chan, A, 1975, The evolution of cannabinoid
phenotypes in Cannabis, ECONOMIC BOTANY 29(3):219-232). The written
record of the pharmacologic properties of Cannabis goes back more
than 4,000 years (Ti, H. 2737 BC. NEI JING SU WEN HUANG TI, Yellow
Emperor's Classic on Internal Medicine; referred to without
citation in Small et al. 1975 Supra).
The taxonomy and nomenclature of the highly variable genus Cannabis
(Emboden, W A, 1974, ECONOMIC BOTANY 28(3):304-310; Small, E and
Cronquist, A, 1976, TAXON 25(4):405-435; Small E and Cronquist, A,
1977, TAXON 26(1):110; Hillig, K W and Mahlberg, P G, 2004,
American Journal of Botany 91(6):966-975), remains in question.
This is in spite of the fact that its formal scientific name,
`Cannabis sativa L.`, assigned by Carolus Linneaus (Linnaeus, C,
1753, SPECIES PLANTARUM, 2:1027, Salvius, Stockholm, Facsimile
edition, 1957-1959, Ray Society, London, U.K.), is one of the
oldest established names in botanical history and is still accepted
to this day. Another species in the genus, `Cannabis indica Lam.`
was formally named somewhat later (Lamarck, J B, 1785, ENCYCLOPEDIE
METHODIQUE DE BOTANIQUE, 1(2):694-695), but is still very old in
botanical history. In 1785, Jean-Baptiste Lamarck published a
description of a second species of Cannabis, which he named
Cannabis indica. Lamarck based his description of the newly named
species on plant specimens collected in India. C. indica was
described as relatively short, conical, and densely branched,
whereas C. sativa was described as tall and laxly branched
(Schultes R. E. et al, 1974, Harvard University Botanical Museum
Leaflets, 23:337-367). C. indica plants were also described as
having short, broad leaflets whereas those of C. sativa were
characterized as relatively long and narrow (Anderson L. C., 1980,
Harvard University Botanical Museum Leaflets, 28: 61-69). C. indica
plants conforming to Schultes' and Anderson's descriptions may have
originated from the Hindu Kush mountain range. Because of the often
harsh and variable (extremely cold winters, and warm summers)
climate of those parts, C. indica is well-suited for cultivation in
temperate climates.
Three other species names were proposed in the 1800s to distinguish
plants with presumably different characteristics (C. macrosperma
Stokes, C. chinensis Delile, C. gigantean Vilmorin), none of which
are accepted today, although the epithet "indica" lives on as a
subspecies of C. sativa (`C. sativa ssp. indica Lam.`, Small and
Cronquist 1976 Supra).
In the 20th century, two new names were added to the liturgy of
proposed `Cannabis species: C. ruderalis` Janischevsky and a
hybrid, x `C. intersita` Sojak. (Small, E, Jui, P Y, and
Lefkovitch, L P, 1976, SYSTEMATIC BOTANY 1(1):67-84; Small and
Cronquist 1976 Supra). Further, numerous names have been proposed
for horticultural variants of `Cannabis` but as of 1976, "very few
of these have been validly published as formal taxa under the
International Code of Botanical Nomenclature" (Small and Cronquist
1976 Supra). Moreover, other recent work continues to focus on
higher-order evolutionary relationships of the genus. Cannabis has
been variously ascribed as belonging to mulberry family (Moraceae)
(Engler, H G A, Ulmaceae, Moraceae and Urticaceae, pages 59-118 in:
A. Engler and K. Prantl eds., 1889, DIE NATURLICHEN
PFLANZENFAMILIEN 3(1). W. Engelmann, Leipzig, Germany; Judd, W S,
Sanders, R W, and Donoghue, M J, 1994, HARVARD PAPERS IN BOTANY
5:1-51; Humphries, C J and Blackmore, S, A review of the
classification of the Moraceae, pages 267-277 In: Crane and
Blackmore 1989 id.); nettle family (Urticaceae) (Berg, C C,
Systematics and phylogeny of the Urticales, pages 193-220, in: P.
R. Crane and S. Blackmore eds., 1989, EVOLUTION, SYSTEMATIC, AND
FOSSIL HISTORY OF THE HAMAMELIDAE, VOL. 2, HIGHER HAMAMELIDAE,
Clarendon Press, Oxford, U.K.); and most recently in its own family
with hops (Humulus), Cannabaceae, or hemp family (Sytsma, K J, et
al, 2002, AMERICAN JOURNAL OF BOTANY 89(9):1531-1546). While the
work of Small and Cronquist 1976 Supra, seemed to effectively
confine the genus to a single species with 2 subspecies (C. sativa
s., C. s. indica), each with two varieties (C. s. s. var. sativa,
C. s. s. var. spontanea; C. s. i. var. indica, C. s. i. var.
Kafiristanica) largely on the basis of chemotaxonomy and
interfertility of all forms, more recent work (Sytsma et al. 2002
Supra), proposes a two-species concept, resurrecting the binomial
C. indica Lam. Since Sytsma et al. (2002) provides no key for
discriminating between the species, the dichotomous key of Small
and Cronquist (1976), which accounts for all forms in nature,
whether wild or domesticated, is preferred to classify the
characteristics of the plants.
BRIEF SUMMARY OF THE INVENTION
This invention relates to a new and distinctive Cannabis cultivar
designated as `OG CITRON`.
The objective of the breeding program which produced novel plants
disclosed herein was primarily to develop a Cannabis cultivar with
its unique blend of various cannabinoids and/or terpenes for (a)
medicinal effects such as improving appetite and reducing nausea,
vomiting and/or chronic pain, as well as neurological and
cardiovascular effects, (b) psychoactive effects such as increased
motivation and energetic behavior rather than indifference,
passiveness and lethargy, and (c) recreational effects with
enhanced enjoyment such as food and aroma.
As used herein, the term "cultivar" is used interchangeably with
"variety", "strain", and/or "clone".
Cannabis plants produce a unique family of terpeno-phenolic
compounds. Cannabinoids, terpenoids, and other compounds are
secreted by glandular trichomes that occur most abundantly on the
floral calyxes and bracts of female plants. As a drug it usually
comes in the form of dried flower buds (marijuana), resin
(hashish), or various extracts collectively known as hashish oil.
The Cannabis plant has at least 545 distinct compounds that span
twenty chemical classes including cannabinoids, terpenes,
terpenoids, amino acids, nitrogenous compounds, simple alcohols,
aldehydes, ketones, esters, lactones, acids, fatty acids, steroids,
non-cannabinoid phenols, pigments, flavonoids, vitamins, proteins,
enzymes, glycoproteins, and hydrocarbons. Terpenes and/or
cannabinoids, in particular, have shown great potential in terms of
medicinal value.
Terpenes and/or cannabinoids have been shown to be largely
responsible for beneficial effects of a Cannabis plant. In fact,
each Cannabis plant has the varying concentrations of medically
viable compounds depending on different strains (genotypes) and
their resulting chemotypes. Even a small variation in terpene
and/or cannabinoid concentration can cause noticeable differences
in the entourage and/or synergistic effects of a Cannabis plant,
which distinguishes one variety from another. Research shows that
it relies heavily on the physiological effects produced by terpenes
and/or cannabinoids.
Over one hundred different kinds of terpenes have been identified
in Cannabis plants although not being as well-studied as
cannabinoids, they are instrumental in giving rise to the
physiological and psychoactive effects in Cannabis.
Terpenes are a large and diverse class of organic compounds,
produced by a variety of plants. They are often strong smelling and
thus may have had a protective function. Terpenes are an important
component, not only influencing taste and smell of each Cannabis
strain but also influencing its effects on the mind and body of a
subject such as humans and animals. Terpenes are a classification
of organic molecules that are found in a wide variety of plants and
animals. These molecules are known for their characteristic scents
and flavors. The varying terpene concentrations found in Cannabis
plants directly influence the resulting taste and smell, as well as
the observed effects. Non-limiting examples of types of terpenes
include Hemiterpenes, Monoterpenes, Sesquiterpenes, Diterpenes,
Sesterterpenes, Triterpenes, Sesquarterpenes, Tetraterpenes,
Polyterpenes, and Norisoprenoids. The main terpenes found in
Cannabis plants include, but are not limited to, myrcene, limonene,
caryophyllene, pinene, terpinene, terpinolene, camphene, terpineol,
phellandrene, carene, humulene, pulegone, sabinene, geraniol,
linalool, fenchol, borneol, eucalyptol, and nerolidol.
Cannabinoids are the most studied group of the main physiologically
active secondary metabolites in Cannabis. The classical
cannabinoids are concentrated in a viscous resin produced in
structures known as glandular trichomes. At least over one hundred
different cannabinoids have been isolated from Cannabis plants. The
main classes of cannabinoids from Cannabis include, but are not
limited to tetrahydrocannabinol (THC), cannabidiol (CBD),
cannabigerol (CBG), and cannabinol (CBN). Cannabinoid can be at
least one of a group comprising tetrahydrocannabinol (THC),
cannabidiol (CBD), cannabigerol (CBG), cannabinol (CBN)
cannabichromene (CBC), cannabinodiol (CBDL), cannabicyclol (CBL),
cannabivarin (CBV), tetrahydrocannabivarin (THCV), cannabidivarin
(CBDV), cannabigerovarin (CBGV), cannabichromevarin (CBCV),
cannabigerol monomethyl ether (CBGM), cannabielsoin (CBE),
cannabicitran (CBT), cannabinol propyl variant (CBNV), cannabitriol
(CBO), and their acidic counterparts, such as
tetrahydrocannabinolic acid (THCA), tetrahydrocannabivarinic acid
(THCVA), cannabidivarinic acid (CBDVA), cannabidiolic acid (CBDA),
cannabigerolic acid (CBGA) and cannabinerolic acid (CBNRA).
Most cannabinoids exist in two forms, as acids and in neutral
(decarboxylated) forms. The acidic form of cannabinoids is
designated by an "A" at the end of its acronym (i.e. THCA). The
cannabinoids in their acidic forms (those ending in "-A") can be
converted to their non-acidic forms through a process called
decarboxylation when the sample is heated. The phytocannabinoids
are synthesized in the plant as acidic forms. While some
decarboxylation does occur in the plant, it increases significantly
post-harvest and the kinetics increase at high temperatures
(Flores-Sanchez and Verpoorte, 2008, Plant Cell Physiol. 49(12):
1767-1782). The biologically active forms for human consumption are
the neutral forms. Decarboxylation is usually achieved by thorough
drying of the plant material followed by heating it, often by
combustion, vaporization, heating, or baking in an oven. Unless
otherwise noted, references to cannabinoids in a plant include both
the acidic and decarboxylated versions (e.g., CBD and CBDA).
The molecules lose mass through the process of decarboxylation. In
order to find the total theoretical active cannabinoids, the acid
forms should be multiplied by 87.7%. For example, THCA can be
converted to active THC using the formula: THCA.times.0.877=THC.
The maximum THC for the sample is:
THC.sub.max=(THCA.times.0.877)+THC. This method has been validated
according to the principles of the International Conference on
Harmonization. Similarly, CBDA can be converted to active CBD and
the yield is determined using the yield formula:
CBDA.times.0.877=CBD. Also the maximum amount of CBD yielded, i.e.
max CBD for the sample is: CBD.sub.max=(CBDA.times.0.877)+CBD.
Additionally, CBGA can be converted to active CBG by multiplying
87.8% to CBGA. Thus, the maximum amount of CBG is:
CBG.sub.max=(CBGA.times.0.878)+CBG.
The biologically active chemicals found in plants, phytochemicals,
may affect the normal structure or function of the human body and
in some cases treat disease. The mechanisms for the medicinal and
psychoactive properties of a Cannabis plant, like any medicinal
herb, produce the pharmacologic effects of its phytochemicals, and
the key phytochemicals for a medical Cannabis plant are
cannabinoids and terpenes.
.DELTA.9-Tetrahydrocannabinol (THC) is a psychoactive cannabinoid
responsible for many of the effects such as mild to moderate pain
relief, relaxation, insomnia and appetite stimulation. THC has been
demonstrated to have anti-depressant effects. The majority of
strains range from about 12-21% THC with very potent and carefully
prepared strains reaching even higher. While
.DELTA.9-Tetrahydrocannabinol (THC) is also implicated in the
treatment of disease, the psychotropic activity of THC makes it
undesirable for some patients and/or indications.
Tetrahydrocannabinol, THC, is the primary psychoactive and
medicinal cannabinoid and is the result of the decarboxylation of
tetrahydrocannabinolic acid (THC-A), its acidic precursor. THC-A,
(6ar,10ar)-1-hydroxy-6,6,9-trimethyl-3-pentyl-6a,7,8,10a-tetrahydro-6h-be-
nzochromene-2-carboxylic acid, is found in the trichomes of the
plant and converted into THC, which actually exists in only minute
quantities in the living plant, after harvest and drying.
Cannabidiol (CBD) is one of the principal cannabinoids found in a
Cannabis plant and is largely considered to be the most medically
significant. CBD occurs in many strains, at low levels, <1%. In
some cases, CBD can be the dominant cannabinoid, as high as 15% by
weight. CBD is non-psychoactive, meaning that unlike THC, CBD does
not cause a noticeable "high". CBD has shown potential for its
medical properties in the treatment of a wide variety of diseases
and symptoms, including cancer, nausea, chronic pain, spasms,
seizures/epilepsy, anxiety, psoriasis, Crohn's disease, rheumatoid
arthritis, diabetes, schizophrenia, post-traumatic stress disorder
(PTSD), alcoholism, strokes, multiple sclerosis, and cardiovascular
disease. CBD also has been reported to act as a muscle relaxant,
antibiotic, anti-inflammatory, and bone stimulant, as well as to
improve blood circulation, cause drowsiness, and protect the
nervous system. It can provide relief for chronic pain due to
muscle spasticity, convulsions and inflammation, as well as
effective relief from anxiety-related disorders. It can offer
relief for patients with Multiple Sclerosis (MS), Fibromyalgia and
Epilepsy. CBD has also been shown to inhibit cancer cell growth
when injected into breast and brain tumors in combination with
THC.
A Cannabis cultivar can be used to achieve the desire of patients
to be treated with CBD without the adverse side-effects (e.g.,
psychoactivity) of THC.
Cannabichromene (CBC) is a rare, non-psychoactive cannabinoid,
usually found at low levels (<1%) when present. It has been
shown to have anti-depressant effects and to improve the
pain-relieving effects of THC. Studies have demonstrated that CBC
has sedative effects such as promoting relaxation.
Cannabidiol (CBD) and cannabichromene (CBC) are both
non-psychoactive and end products of CBG metabolism, like THC, so
that they can be used medically.
Cannabigerol (CBG) is a non-psychoactive cannabinoid. CBG-acid is
the precursor to both THC-acid and CBD-acid in the plant usually
found at low levels (<1%) when present. It has been demonstrated
to have both pain relieving and inflammation reducing effects. CBG
reduces intraocular pressure, associated with glaucoma. CBG has
been shown to have antibiotic properties and to inhibit platelet
aggregation, which slows the rate of blood clotting. While
Cannabigerol (CBG), is not considered psychoactive, it is known to
block the psychoactive effects of THC and is considered medically
active in a variety of conditions. Its precursor, cannabigerolic
acid, CBG-A,
(E)-3-(3,7-Dimethyl-2,6-octadienyl)-2,4-dihydroxy-6-pentylbenzoic
acid, is being studied medically.
Cannabinol (CBN) is an oxidative degradation product of THC. It may
result from improper storage or curing and extensive processing,
such as when making concentrates. It is usually formed when THC is
exposed to UV light and oxygen over time. CBN has some psychoactive
properties, less strength than THC. CBN is thought to enhance the
dizziness and disorientation that users of Cannabis may experience.
It may cause feelings of grogginess, and has been shown to reduce
heart rate.
High potency Cannabis plants contain large quantities of specific
terpenes as well as various assortments of other terpenes. For
instance, a Cannabis plant may have a profile with either a high
level of, a moderate amount of, or a small amount of various
terpenes depending on its cultivar and environmental
conditions.
Various cultivars of `Cannabis` species have been cultivated in an
effort to create a cultivar best suited to meet the interest of
inventors according to their own need. The particular plant
disclosed herein was discovered in the area where the inventors
were intentionally cross-pollinating and cultivating plants
described below using standard Mendelian breeding procedures well
known to those of ordinary skill in the art. This resulted in the
progenies of the inventors' crosses.
The progenies resulting from any selection stage of either the
crossing, selfing or backcrossing versions of the breeding regimes
of the present invention were asexually reproduced to fix and
maintain the desirable THC content, CBs content, terpenes content,
the aroma and flavor(s) typical of the desired class, and the other
desirable phenotypic and/or genotypic characteristics. The
resultant selected Cannabis cultivar is designated as `OG CITRON`
disclosed herein.
The inventors reproduced progenies asexually by stem cutting and
cloning. This is the origin of this remarkable new cultivar. The
plant has been and continues to be asexually reproduced by stem
cutting and cloning at the inventors' greenhouses, nurseries and/or
fields in Salinas, Calif., Oakland, Calif., and/or Washington,
D.C.
The following are the most outstanding and distinguishing chemical
characteristics of this new cultivar when grown under normal
conditions in Salinas, Calif. Chemical analyses of the new Cannabis
variety and the check variety (or the parental varieties) disclosed
herein were performed using standard chemical separation techniques
well known to those skilled in the art. Samples for assaying were
obtained from flower tissues of the Cannabis plant disclosed
herein. Cannabinoid composition of this cultivar can be determined
by assaying the concentration of at least one cannabinoid in a
subset (e.g., sample) of the harvested product.
Table 1 includes detailed information of the Cannabis plant named
`OG CITRON` including the concentration ranges of terpenes and
cannabinoids as tested on flowers at least twelve different times.
The Cannabis plant has been tested in a laboratory setting and/or
facility to determine cannabinoids and terpenes concentrations in
the Cannabis plant named `OG CITRON` according to the procedures
provided in Giese et al. (Journal of AOAC International (2015)
98(6):1503-1522). High throughput homogenization (HTH) of typical
inflorescences as a single sample preparation method for the
analysis of both terpenoids and cannabinoids. HPLC-UV assay was
used for HTH of cannabinoids, while GC-flame ionization detector
(FID) was used for HTH of terpenes. 1) The main terpenes found in
`OG CITRON` are linalool, limonene, myrcene, beta-caryophyllene,
alpha-bisabolol, alpha-humulene, and beta-pinene; and 2) The
estimated concentration of the total THC.sub.max, CBD.sub.max, and
CBG.sub.max is about 9.90-12.66%, about 5.50-9.23%, and about
0.08-0.78%, respectively, at the time of assaying metabolites from
flower samples of `OG CITRON`.
Terpene and cannabinoid profiles of `OG CITRON` demonstrate that
`OG CITRON` has a phenotypically unique profile, particularly
insofar as to the level of terpenes and cannabinoids. This data is
presented in a tabular form in Table 1.
TABLE-US-00001 TABLE 1 Ranges of Active Cannabinoids and Terpenes
Ranges of Active Cannabinoids (% by weight) Max THC 9.90- Max CBD
5.50- Max CBG 0.08- 12.66% 9.23% 0.78% Ranges of Terpenes (% by
weight) thujene 0.00% gamma- 0.00% hexyl 0.00- terpinene hexanoate
0.03% alpha-pinene 0.02- linalool oxide 0.00- octyl butyrate 0.00%
0.05% 0.02% camphene 0.00- terpinolene 0.00- beta- 0.21- 0.02%
0.01% caryophyllene 0.32% sabinene 0.00% fenchone 0.00% alpha-
0.09- humulene 0.14% beta-pinene 0.05- linalool 0.24- cis-nerolidol
0.00% 0.10% 0.71% myrcene 0.11- fenchol 0.02- trans-nerolidol 0.00%
0.47% 0.07% alpha- 0.00% -- -- caryophyllene 0.01- phellandrene
oxide 0.04% carene 0.00% camphor 0.00% alpha- 0.00- bisabolol 0.16%
alpha- 0.00% isoborneol 0.00% nerol 0.00% terpinene limonene 0.26-
(-)borneol 0.00- geraniol 0.00% 0.64% 0.02% beta- 0.00% menthol
0.00% geranyl- 0.00% phellandrene acetate cineole 0.00% hexyl 0.00%
methyl- 0.00% butyrate eugenol cis-ocimene 0.00% alpha- 0.00- Total
1.67- terpineol 0.07% Terpenes 2.57% trans-ocimene 0.00%
citronellol 0.00% -- --
The Cannabis plant named `OG CITRON` has a complement of terpenes,
including but not limited to, relatively high levels of linalool,
limonene, myrcene, beta-caryophyllene, alpha-bisabolol,
alpha-humulene, and beta-pinene compared to other terpene
compounds. This unique combination of differently concentrated
terpenes further distinguishes `OG CITRON` from other varieties in
its odor, its medical qualities, and its effects on mood and
mentation.
Asexual Reproduction
Asexual reproduction, also known as "cloning", is a process well
known to those of ordinary skill in the art of Cannabis production
and breeding and includes the following steps.
The Cannabis cultivar disclosed herein is asexually propagated via
taking cuttings of shoots and putting them in rock wool cubes.
These cubes are presoaked with pH-adjusted water and kept warm
(-80.degree. F.). Full trays are covered, left under 18 hours of
light and allowed to root (7-14 days). Upon root onset, the
plantlets are transplanted into rigid 1 gallon containers filled
with a proprietary soil mix A and remain in 18 hours of daylight
for another 14-21 days. Once root-bound, plants are transplanted
into rigid 3 gallon containers filled with proprietary soil mix B.
Immediately, the light cycle is altered to 12/12 and flower
initiating begins. The plants remain in 12/12 lighting until
harvesting. They undergo a propriety nutrient regimen and grow as
undisturbed as possible for 60-70 days depending on chemotype
analysis.
All sun leaves are removed and the plant is dismantled to result in
approximately 12'' branches covered in inflorescences and
trichomes. The goal in harvesting is to actually harvest trichome
heads but not `buds`. Thus, great care is taken not to disturb the
trichome heads and as much of the plant remains intact as possible
to promote even and slow drying. Slow drying is followed by a one
to two months curing process.
Observation of the all female progenies of the original plant has
demonstrated that this new and distinct cultivar has fulfilled the
objectives and that its distinctive characteristics are firmly
fixed and hold true from generation to generation vegetatively
propagated from the original plant.
Under careful observation, the unique characteristics of the new
cultivar have been uniform, stable and reproduced true to type in
successive generations of asexual reproduction.
DESCRIPTION OF THE DRAWINGS
The accompanying color photographs depict characteristics of the
new `OG CITRON` plants as nearly true as possible to make color
reproductions. The overall appearance of the `OG CITRON` plants in
photographs is shown in colors that may differ slightly from the
color values described in the detailed botanical description.
FIG. 1 shows an overall view of the `OG CITRON` plant from the
side.
FIG. 2A shows a close view of a single leaf of the check variety
BLK03 plant.
FIG. 2B shows a close view of a single leaf of the new variety `OG
CITRON` plant.
FIG. 3A shows top parts (including inflorescence) of the BLK03
plant from the side.
FIG. 3B shows top parts (including inflorescence) of the `OG
CITRON` plant from the side.
FIG. 4 shows a close view of flowers of the `OG CITRON` plant at
the mid to late flowering stage.
FIG. 5 shows another close view of flowers of the `OG CITRON` plant
at the mid to late flowering stage.
FIG. 6 shows a reproductive part of a sample Cannabis plant,
indicating position/location of a flower, a bract and a stipule in
the plant. The sample Cannabis plant in FIG. 6 is not the claimed
`OG CITRON` plant.
DETAILED BOTANICAL DESCRIPTION
`OG CITRON ` has not been observed under all possible environmental
conditions, and the phenotype may vary significantly with
variations in environment. The following observations,
measurements, and comparisons describe this plant as grown at
Salinas, Calif., when grown in the greenhouse, nursery or field,
unless otherwise noted.
Plants for the botanical measurements in the present application
are annual plants. In the following description, the color
determination is in accordance with The Royal Horticultural Society
Colour Chart, 2007 Edition, except where general color terms of
ordinary dictionary significance are used.
The Cannabis plant disclosed herein was derived from female and
male parents that are internally designated as below.
The internal GNBR Code of the Cannabis plant named `OG CITRON` is
S8.08.34, also known as SLV08.P38.BX.08.34. The internal GNBR
Breeding Code of the Cannabis plant named `OG CITRON` is
(S08)x(P38.BX.08).34. The additional number `.34` was only assigned
to the 34.sup.th individual plant (i.e.`OG CITRON`) selected from
progenies of the cross event between pollen acceptor (S08) and
pollen donor (P38.BX.08). `OG CITRON` is a fertile hybrid derived
from a controlled-cross between two proprietary cultivars: (i)
SLV08 (pollen acceptor; female parent), also known as S08 or S8,
and (ii) P38.BX.08 (pollen donor; male parent), also known as 08.
The initial cross between two parental cultivars was made in
September 2015. The primary phenotypic criteria used to select the
new and distinct Cannabis cultivar disclosed herein is as follows:
structure score, nose/organoleptic testing, mold
susceptibility/resistance, and insect susceptibility/resistance.
Also, the chemotypic characteristics described in Table 1 were used
to select the new and distinct Cannabis cultivar disclosed herein.
The first asexual propagation of `OG CITRON` occurred on Apr. 30,
2016 in Salinas, Calif.
The following traits in combination further distinguish the
Cannabis cultivar `OG CITRON` from the check variety `BLK03`, which
is set as a standard for phenotypic comparison. Tables 2 to 6
present phenotypic traits and/or characteristics of `OG CITRON`
compared to the check variety `BLK03` as follows. Unless otherwise
indicated, all plants were raised together and evaluated when 90
days old (i.e., 23 days in vegetative stage, 15 days in clonal
propagation stage, and 52 days in flowering times).
TABLE-US-00002 TABLE 2 General Characteristics Characteristics New
Variety Check Variety (BLK03) Plant life An herbaceous An
herbaceous forms plant (herb) plant (herb) Plant growth An upright,
tap-rooted An upright, tap-rooted habit annual plant; forming
annual plant; forming fibrous roots when fibrous roots when
asexually propagated asexually propagated Plant origin A
controlled-cross between A controlled-cross between pollen acceptor
(SLV08) pollen acceptor and (P38.BX.08) (GLD13) and pollen donor
(BSIA) Plant Asexually propagated by Asexually propagated by
propagation stem cuttings and cloning stem cuttings and cloning
Propagation Easy Moderate ease Height 1.50-2.30 m 0.50-2.50 m Width
89.0 cm 119.5 cm Plant vigor High Medium Time to 7-8 weeks; as
early 8 weeks Harvest as 50 days (Seed to Harvest) Resistance to
Resistant to pests as Not Resistant to pests as pests or follows;
(1) Two-spotted follows; (1) Two spotted diseases spider mite
(Tetranychus spider mite urticae (Koch)); (Tetranychus urticae (2)
Aphids species such (Koch)); (2) Aphids as: Cannabis species such
as: Cannabis Aphids (Phorodon Aphids (Phorodon cannabis), Green
Peach cannabis), Green Peach Aphid (Myzus persicae Aphid (Myzus
(Sulzer)), Foxglove Aphid persicae (Sulzer)), (Aulacorthum solani),
Foxglove Aphid (Aulacor- Peach Aphid thum solani), Peach Aphid
(Macrosiphum (Macrosiphum euphorbiae), Black Bean euphorbiae),
Black Aphid (Aphis fabae); Bean Aphid (Aphis (3) Whitefly
(Trialeurodes fabae); (3) Whitefly (Tri- vaporariorum; (4) Lepi-
aleurodes vaporariorum); doptera species such as: (4) Lepidoptera
species Armyworm (Spodoptera such as: Armyworm frupperda), Cabbage
(Spodoptera frupperda), Whites (Pieris rapae), Cabbage Whites
(Pieris Painted Lady (Vanessa rapae), Painted cardui), Lepidoptera
sp. Lady (Vanessa Resistant to diseases cardui), Lepidoptera sp. as
follows;Powdery Mildew Not resistant to diseases (Podosphaera
xanthii) as follows; Botrytis/ Flower Rot (Botrytis cinerea) and
Powdery Mildew (Podosphaera xanthii) Genetically- NO NO modified
organism
TABLE-US-00003 TABLE 3 Leaf/Foliage Characteristics New Variety
Check Variety (BLK03) Leaf Alternate Alternate arrangement Leaf
shape Palmately compound Palmately compound Leaf structure
Linear-lanceolate Linear-lanceolate leaflet blades leaflet blades
with glandular hairs with glandular hairs Leaf margins Dentate,
coarsely Dentate, coarsely serrated, serrated, and the teeth and
the teeth point point away from the tip away from the tip Leaf
hairs Present and abundant Present on both upper on both upper and
lower surfaces and lower surfaces Leaf length with 40.20 cm 16.60
cm petiole at maturity Leaf width at 9.3-15.9 cm 10.7 cm maturity
Petiole length at 17.10 cm 6.50 cm maturity Petiole color 149A 140C
(RHS No.) Intensity of Very weak Medium (vegetative petiole stage);
very strong anthocyanin (late flowering stage) Stipule length at
0.70 cm 0.70 cm maturity Stipule shape Bulbous Elliptical Stipule
color 134A 149B (RHS No.) No. of leaflets 5-7 5-7 Middle largest
23.50 cm 9.80 cm (longest) leaflet length Middle largest 4.70 cm
2.3 cm (longest) leaflet width Middle largest 23.5:4.7 9.8:2.3
(longest) leaflet length/width ratio No. teeth of 40 25 middle
leaflet (average) Leaf (upper 132B 132A side) color (RHS No.) Leaf
(lower 141D 134D side) color (RHS No.) Leaf glossiness Medium at
the upper Strong at the upper leaf surface leaf surface Vein/midrib
Obliquely continuous Obliquely continuous shape throughout leaflet
throughout leaflet Vein/midrib 150B 144C color (RHS No.) Aroma
Euphoric to Chemical/ Spicy Ambrosial
TABLE-US-00004 TABLE 4 Stem Characteristics New Variety Check
Variety (BLK03) Stem shape Hollow and large Hollow, ribbed,
textured Stem diameter 3.40 cm 2.80 cm at base Stem color 149D
N144D (RHS No.) Depth of main Shallow Absent stem ribs/grooves
Internode length 9.9-17.3 cm 2.4-4.9 cm
TABLE-US-00005 TABLE 5 Inflorescence (Female/Pistillate Flowers)
Characteristics New Variety Check Variety (BLK03) Flowering Cymes
Cymes (blooming) habit Proportion of 100% pistillate 100%
pistillate female plants Inflorescence Even Even position Flower
Cymose (Inflorescences are Cymose (terminal bud arrangement very
compact, and there- matures, while lateral fore bracts overlap
within flowers mature thereafter) each cluster of flowers. Thus,
the flowers are over- lapping within each node) Number of 25-50
80-120 flowers per plant Flower shape Calcarate-urceolate
Calcarate-urceolate Flower 0.61 cm 0.70 cm (individual pistillate)
length Flower 9.70 cm 3.80 cm (compound cyme) diameter Corolla
shape No defined corolla No defined corolla Corolla Color N/A N/A
(RHS No.) Bract shape Urceolate Urceolate Bract size 0.8-2.0 cm
(high 0.2-0.8 cm density of trichomes) Bract color 130A N134C (RHS
No.) Calyx shape No defined calyx No defined calyx Calyx color N/A
N/A (RHS No.) Stigma shape Linear Acute Stigma length 0.1-0.4 mm
2.20 mm Stigma color 30A 159D (RHS No.) Trichome Capitate-stalked
glandular Capitate-stalked shape glandular Trichome 157A at day 55
in flowering 157A at day 40 in color (before harvest) flowering
(RHS No.) Other types of Capitate sessile trichomes Capitate
sessile trichomes trichomes are present on the upper are present on
the leaves of and lower surfaces on plants, as well as being leaves
of plants, noticed in the flowers as well as being (color: 157A at
day 40 noticed in the flowers in flowering). (color: 157A at day
During later flowering, i.e. 55 in flowering). day 48 to day 60 in
During later flowering, flowering, capitate stalked i.e. day 60
trichomes are present to day 70 in flowering, (color: N30B). the
capitate stalked Bulbous and trichomes are present non-glandular
trichomes are (color: N30B). also present and most notice- able on
the petioles, stems, and leaves (color: 157A). Terminal bud
Urceolate Oblong shape Terminal bud 127B 203C color (RHS No.)
Pedicel Absent Absent Staminate No staminate flowers No staminate
flowers shape produced naturally; produced naturally; however, male
flower however, male flower (staminate) can be induced (staminate)
can be induced with chemical compounds with chemical compounds
(such as silver nitrate (such as silver nitrate and silver
thiosulphate and silverthiosulphate anionic complex). anionic
complex). Pollen Absent Absent description Seed shape Striped
Smooth and globular Seed size 2.80-3.20 mm 1.80-2.30 mm (Diameter)
Marbling of Weak to absent Absent (non-existent) seed Petal
Apetalous Apetalous description Max THC About 9.90-12.66% About
18.88-19.37% content Max CBD About 5.50-9.23% 0.00% content Max CBG
About 0.08-0.78% About 0.84-0.91% content N/A: Not available
TABLE-US-00006 TABLE 6 Other Characteristics Characteristics New
Variety Check Variety (BLK03) Time period and 7-9 weeks 7-9 weeks
condition of flowering/ blooming Hardiness of Hardy to 25.degree.
F.- Hardy to 25.degree. F.- plant ambient temperature ambient
temperature Breaking action Flexible, resistant Strong,
non-flexible to breakage Rooting rate 99%-vigorous 70%-moderate
after cutting/cloning Types of Stem Stem Cutting for Cloning
Shipping quality Good Moderate Storage life Medium (3-6 months
Medium (2-6 months with minor changes with minor changes in
physical appearance in physical appearance and/or smell/taste)
and/or smell/taste) Market use Medicinal n/a
In general, `OG CITRON` is larger in width and height than both
parents, pollen acceptor (S08) and pollen donor (P38.BX.08). `OG
CITRON` is more robust in terms of growing performance, time to
rooted clones, and time to flower maturity. As `OG CITRON` has
greater resistance to pests and disease (specifically to powdery
mildew), it yields higher than both parents. Since `OG CITRON` has
stronger branches and thicker stems with greater flexibility than
both parents, its main and lateral branches give `OG CITRON`
ability to produce higher yields under different/variable growing
conditions. The flowers of `OG CITRON` are larger in width and
length than both parents. `OG CITRON` clearly demonstrates hybrid
vigor, and outperforms both parents overall. Chemically, `OG
CITRON` has a higher cannabinoid content with a high THC:CBD ratio
as well as a higher terpene content than either parent. Also, `OG
CITRON` has unique combination of parental terpenes.
When `OG CITRON` is compared to the check variety `BLK03`, `OG
CITRON` is narrower in width than `BLK03` and has higher plant
vigor than `BLK03`. `OG CITRON` has longer and wider leaves than
`BLK03` in terms of whole leaf length including petioles. Also, `OG
CITRON` has longer and wider leaflets than `BLK03` when comparing
the middle largest leaflet. `OG CITRON` has more than twice longer
petioles in average than `BLK03` at maturity, while it has about
the same stipule size to `BLK03`. Regarding the average stem
diameter at base, `OG CITRON` is longer than `BLK03`, and the
internode length of `OG CITRON` is also longer than that of
`BLK03`. When comparing the compound cyme diameter, `OG CITRON` is
longer than `BLK03`, while individual pistillate flowers of `OG
CITRON` are shorter than that of `BLK03` in length. `OG CITRON` has
longer bracts than `BLK03`, while having shorter stigma. With
respect to aroma, `OG CITRON` has an ambrosial smell, while `BLK03`
has a generally spicy smell.
When `OG CITRON` is compared to the known Cannabis plant named
`ECUADORIAN SATIVA` (U.S. Plant Pat. No. 27,475), there are several
distinctive characteristics. For example, the overall form of `OG
CITRON` plant is taller in height and wider across at the widest
point than `ECUADORIAN SATIVA` plant. `OG CITRON` plant has longer
leaves including petioles, longer and wider middle leaflets, and
longer petioles at maturity than `ECUADORIAN SATIVA` plant.
Regarding stem diameter at base, `OG CITRON` pant is longer than
`ECUADORIAN SATIVA` plant. When comparing individual pistillate
flower length, `OG CITRON` flowers are longer than `ECUADORIAN
SATIVA` flowers. While the aroma of `ECUADORIAN SATIVA` is strongly
mephitic with hints of limonene, `OG CITRON` has an ambrosial
smell. When comparing total THC content between `OG CITRON` and
`ECUADORIAN SATIVA`, the total THC content of `OG CITRON` is about
9.90-12.66%, while `ECUADORIAN SATIVA` accumulates 12.45% total
THC.
* * * * *
References