U.S. patent number PP27,141 [Application Number 13/998,648] was granted by the patent office on 2016-09-13 for corylus plant named `wepster`.
This patent grant is currently assigned to State of Oregon Acting by and through the State Board of Higher Education on behalf of Oregon State University. The grantee listed for this patent is Rebecca L. McCluskey, Shawn A. Mehlenbacher, David C. Smith. Invention is credited to Rebecca L. McCluskey, Shawn A. Mehlenbacher, David C. Smith.
United States Patent |
PP27,141 |
Mehlenbacher , et
al. |
September 13, 2016 |
Corylus plant named `Wepster`
Abstract
A new and distinct cultivar of Corylus plant named `Wepster`
characterized by high vigor and an upright-spreading plant habit,
yellowish-green developing and fully expanded leaves during the
spring and summer, resistance to eastern filbert blight caused by
the fungus Anisogramma anomala (Peck) E. Muller, presence of random
amplified polymorphic DNA markers 152-800 and 268-580 amplified by
the polymerase chain reaction and which are linked to a dominant
allele for resistance to eastern filbert blight from the cultivar
Gasaway, expression of incompatibility alleles S.sub.1 and S.sub.2
in the styles, and DNA fingerprints at 8 of 20 microsatellite
marker loci differ from both parents OSU 440.005 and `Tonda
Pacifica`, and from one parent at an additional 12 marker loci.
Inventors: |
Mehlenbacher; Shawn A.
(Corvallis, OR), Smith; David C. (Corvallis, OR),
McCluskey; Rebecca L. (Corvallis, OR) |
Applicant: |
Name |
City |
State |
Country |
Type |
Mehlenbacher; Shawn A.
Smith; David C.
McCluskey; Rebecca L. |
Corvallis
Corvallis
Corvallis |
OR
OR
OR |
US
US
US |
|
|
Assignee: |
State of Oregon Acting by and
through the State Board of Higher Education on behalf of Oregon
State University (Corvallis, OR)
|
Family
ID: |
53174710 |
Appl.
No.: |
13/998,648 |
Filed: |
November 19, 2013 |
Prior Publication Data
|
|
|
|
Document
Identifier |
Publication Date |
|
US 20150143594 P1 |
May 21, 2015 |
|
Current U.S.
Class: |
PLT/152 |
Current CPC
Class: |
A01H
5/08 (20130101); A01H 6/54 (20180501) |
Current International
Class: |
A01H
5/08 (20060101) |
Field of
Search: |
;PLT/152,216 |
Other References
Bassil et al, "Additional Microsatellite Markers of the European
Hazelnut," Acta Hortic. 686:105-110, 2005. cited by applicant .
Bassil et al, "Microsatellite Markers in Hazelnut: Isolation,
Characterization, and Cross-species Amplification," J. Amer. Soc.
Hort. Sci., 130(4):543-549, 2005. cited by applicant .
Bassil et al, "Nuclear and chloroplast microsatellite markers to
assess genetic diversity and evolution in hazelnut species, hybrids
and cultivars," Genet. Resour. Crop Evol., 60(2):543-568, 2012.
cited by applicant .
Boccacci et al, "Characterization and evaluation of microsatellite
loci in European hazelnut (Corylus avellana L.) and their
transferability to other Corylus species," Molecular Ecology Notes,
5:934-937, 2005. cited by applicant .
Boccacci et al, "DNA typing and genetic relations among European
hazelnut (Corylus avellana L.) cultivars using microsatellite
markers," Genome, 49:598-611, 2006. cited by applicant .
Gokirmak et al, "Characterization of European hazelnut (Corylus
avellana) cultivars using SSR markers," Genet. Resour. Crop Evol.,
vol. 56(2), pp. 147-172, 2008. cited by applicant .
Gurcan et al, "Genetic diversity in hazelnut (Corylus avellana L.)
cultivars from Black Sea countries assessed using SSR markers,"
Plant Breeding, 129:422-434, 2010. cited by applicant .
Gurcan et al, "Development, characterization, segregation, and
mapping of microsatellite markers for European hazelnut (Corylus
avellana L.) from enriched genomic libraries and usefulness in
genetic diversity studies," Tree Genetics & Genomes, 6:513-531,
2010. cited by applicant .
Gurcan et al, "Transferability of Microsatellite Markers in the
Betulaceae," J. Amer. Soc. Hort. Sci. 135(2):159-173, 2010. cited
by applicant .
Gurcan and Mehlenbacher. "Development of microsatellite marker loci
for European hazelnut (Corylus avellana L.) from ISSR fragments,"
Molecular Breeding 26:551-559, 2010. cited by applicant .
Mehlenbacher et al., "Inheritance of the Cutleaf Trait in
Hazelnut," HortScience, 30(3):611-612, 1995. cited by applicant
.
Mehlenbacher et al., "`Tonda Pacifica` hazelnut," HortScience
46:505-508, 2011. cited by applicant .
Mehlenbacher et al., "`Santiam` hazelnut," HortScience 42:715-717,
2007. cited by applicant .
Mehlenbacher et al., "RAPD markers linked to eastern filbert blight
resistance in Corylus avellana," Theor. Appl. Genet., 108:651-656,
2004. cited by applicant .
Sathuvalli et al., "Characterization of American hazelnut (Corylus
americana) accessions and Corylus americana.times.Corylus avellana
hybrids using microsatellite markers," Genet. Resour. Crop. Evol.,
59:1055-1075, 2012. cited by applicant.
|
Primary Examiner: McCormick Ewoldt; Susan
Assistant Examiner: Redden; Karen
Attorney, Agent or Firm: Klarquist Sparkman, LLP
Government Interests
ACKNOWLEDGMENT OF GOVERNMENT SUPPORT
This invention was made with government support under Specific
Cooperative Agreement No. 58-5358-9-447 awarded by the United
States Department of Agriculture. The government has certain rights
in the invention.
Claims
We claim:
1. A new and distinct cultivar of Corylus plant named `Wepster`, as
illustrated and described.
Description
Botanical denomination: Corylus avellana.
Variety designation: `Wepster`.
BACKGROUND
The present Invention relates to a new and distinct cultivar of
Corylus plant, botanically known as Corylus avellana, and
hereinafter referred to by the name `Wepster`. Corylus avellana is
in the family Betulaceae.
The new Corylus resulted from a controlled cross of female parent
`Tonda Pacifica` and male parent OSU 440.005 (unpatented) made in
1997 by Shawn A. Mehlenbacher and David C. Smith. `Tonda Pacifica`
is protected by U.S. Plant Pat. No. 22,715 (issued May 8, 2012).
Hybrid seeds from the cross were harvested in August 1997,
stratified, and seedlings grown in the greenhouse during the summer
of 1998. From this cross, a total of 182 seedling trees were
planted in the field in Corvallis, Oreg., USA in October, 1998.
`Wepster` was discovered and selected by the Inventors as a single
plant within the progeny of the stated cross-pollination in a
controlled environment in Corvallis, Oreg. It was originally
assigned the designation OSU 894.030 (unpatented), which indicates
the row and tree location of the original seedling. OSU 440.005
(unpatented) is from a cross of OSU 49.073.times.VR 8-32 (both
unpatented). OSU 49.073 is from a cross of OSU 14.084.times.`Tombul
Ghiaghli` (both unpatented). OSU 14.084 is from a cross of
`Barcelona`.times.`Daviana` (both unpatented). VR 8-32 is from a
cross of `Montebello`.times.`Gasaway` (both unpatented). `Tonda
Gentile delle Langhe` (unpatented), the female parent of `Tonda
Pacifica`, is an important cultivar in Piemonte, northern Italy.
`Barcelona`, Oregon's most widely planted hazelnut cultivar, is
known as `Castanyera` (unpatented) where it originated in
Catalunya, Spain. `Daviana`, originally from England, is the most
widely planted pollinizer in `Barcelona` orchards. `Tombul
Ghiaghli`, obtained from Greece, is similar to the Turkish cultivar
`Mincane` (unpatented). `Montebello` is widely grown under several
names in Sicily. `Gasaway` is the source of a single dominant gene
for resistance to eastern filbert blight.
The new cultivar was asexually reproduced by rooted suckers
annually for eight years (2004-05 and 2007-12) in Corvallis, Oreg.
The new cultivar was also asexually propagated by whip grafting in
2005 in Corvallis, Oreg. The unique features of this new Corylus
are stable and reproduced true-to-type in successive generations of
asexual reproduction.
SUMMARY
The following traits have been repeatedly observed and are
determined to be the unique characteristics of `Wepster`. These
characteristics in combination distinguish `Wepster` as a new and
distinct cultivar: 1. High vigor and upright-spreading plant habit.
2. Yellowish-green developing and fully expanded leaves during the
spring and summer. 3. Resistance to eastern filbert blight caused
by the fungus Anisogramma anomala (Peck) E. Muller. 4. Presence of
random amplified polymorphic DNA markers 152-800 and 268-580 in DNA
of `Wepster` amplified by the polymerase chain reaction. These two
markers are linked to a dominant allele for resistance to eastern
filbert blight from the cultivar Gasaway (unpatented). 5.
Expression of incompatibility alleles S.sub.1 and S.sub.2 in the
styles. 6. DNA fingerprints at 8 of 20 microsatellite marker loci
differ from both parents, `Tonda Pacifica` and OSU 440.005, and
from one parent at an additional 12 marker loci. Additional DNA
fingerprints of grandparent `Tonda Gentile delle Langhe`, standard
cultivar `Barcelona`, eastern filbert blight resistance source
`Gasaway` and five cultivars released by Oregon State University
hazelnut breeding program are shown in attached Table 2.
In comparisons in two replicated trials conducted in Corvallis,
Oreg., plants of the new Corylus differed from plants of the
Corylus avellana cultivar `Barcelona` (unpatented), and other
cultivars and selections of Corylus avellana known to the Inventors
primarily in nut size, nut shape, kernel percentage (ratio of
kernel weight to nut weight), frequency of blank nuts (nuts lacking
kernels), time of pollen shed, time of nut maturity, length of the
husk or involucre, and plant size. For example: Blank nut
frequency: `Wepster` in two trials 7.25%, `Barcelona` in one trial
7.7%; Pollen shed: `Wepster` sheds pollen about 6 days later than
`Barcelona` (average of 3 years, 2011-13); Nut maturity: `Wepster`
nuts mature 8 days earlier than Barcelona; and Husk Length:
`Wepster` 2 times nut length, `Barcelona` 1.5 times nut length.
BRIEF DESCRIPTION OF THE DRAWINGS
The accompanying colored photographs illustrate the overall
appearance of the new cultivar, showing the colors as true as it is
reasonably possible to obtain in colored reproductions of this
type. Colors in the photographs may differ slightly from the color
values cited in the detailed botanical description which accurately
describe the colors of the new Corylus.
FIG. 1 shows typical nuts, raw kernels, and blanched kernels of
`Wepster` hazelnut (OSU 894.030) compared to those of `Yamhill`
hazelnut.
FIG. 2 shows the typical nuts, raw kernels, and blanched kernels of
`Wepster` hazelnut (OSU 894.030) compared to those of `Barcelona`,
`Felix,` and `York` hazelnut cultivars.
FIG. 3 shows the blanched kernels of `Wepster` hazelnut (OSU
894.030).
FIG. 4 shows the tree of the new cultivar `Wepster` growing in a
field in January, in Corvallis, Oreg.
FIG. 5 shows a pruned tree of the new cultivar `Wepster` in
January, in Corvallis, Oreg.
FIG. 6 shows the husks and nuts of `Wepster` hazelnut.
FIG. 7 shows a tree of the new cultivar `Wepster,` 8.sup.th leaf,
growing in a field in the summer, in Corvallis, Oreg.
DETAILED DESCRIPTION
The cultivar `Wepster` has not been observed under all possible
environmental conditions. The phenotype may vary somewhat with
variations in environment such as temperature and light intensity,
without, however, any variance in genotype. The aforementioned
images and following observations and measurements describe plants
grown in Corvallis, Oreg. under commercial practice outdoors in the
field during the fall, winter and spring. Plants used for the
photographs and description were propagated by tie-off layerage and
growing on their own roots, and about seven years old. In the
following description, color references are made to The Royal
Horticultural Society Colour Chart, 1966 Edition, except where
general terms of ordinary dictionary significance are used. The
list of UPOV descriptors are from the Mar. 28, 1979 Hazelnut
guidelines from UPOV. Botanical classification: Corylus avellana
cultivar Wepster. Parentage: Female, or seed, parent.--Corylus
avellana cultivar Tonda Pacifica (U.S. Plant Pat. No. 22,715).
Male, or pollen, parent.--Corylus avellana selection OSU 440.005
(unpatented). Propagation (type rooted suckers): Time to initiate
roots.--About 30 days at 20.degree. C. Time to produce a rooted
young plant.--About six months at 22.degree. C. Root
description.--Fine to thick; freely branching; creamy white in
color. Propagation (type whip grafting): Time to budbreak on the
scions.--About 14 days at 25.degree. C. Time to produce a grafted
plant.--About six months at 25.degree. C. Plant description:
Type.--Natural habit is a perennial shrub, but in commercial
orchards is a single-trunk tree. Upright-spreading plant habit.
Growth and branching habit.--Freely branching; about 15 lateral
branches develop per plant. Pinching, i.e., removal of the terminal
apices, enhances branching with lateral branches potentially
forming at every node. Size.--Plant height. -- About 6 meters;
plant diameter or spread is about 6 meters. Vigor.--High vigor
growth habit. Lateral branch description: Length.--About 32 cm.
Diameter.--About 6 mm. Internode length.--About 3.0 cm.
Texture.--Smooth, glabrous. Strength.--Strong. Color.--Immature --
152B; mature -- 152B. Foliage description: Arrangement.--Alternate,
simple. Length.--About 10.2 cm. Width.--About 9.1 cm.
Shape.--Oblong to ovate. Apex.--Obtuse to acute. Base.--Cordate.
Margin.--Serrate. Texture, upper and lower surfaces.--Slightly
pubescent. Venation pattern.--Pinnate. Leaf bud shape.--Globular.
Time of leaf budbreak.--Descriptor=6 (medium to late). Coloration
of leaf bud.--178C. Time of leaf fall.--Descriptor=4 (between
`Tonda Gentile delle Langhe` and `Barcelona`). Color.--Developing
foliage, upper surface 144A, lower surfaces: 187A. Fully expanded
foliage, upper surface: Spring and summer, 143A; late summer and
fall, 143A. Fully expanded foliage, lower surface: Spring and
summer, 139C; late summer and fall, 139C. Venation, upper surface:
Spring and summer, 139C; late summer and fall, 139C. Venation,
lower surface: Spring and summer, 139D; late summer and fall, 139D.
Petiole description: Length.--About 2.7 cm. Diameter.--About 1.8
mm. Texture, upper and lower surfaces.--Pubescent. Color.--Upper
surface: Spring and summer, 139D; late summer and fall, 139D. Lower
surface: Spring and summer, 139D; late summer and fall, 139D.
Flower description: Male inflorescences.--Catkins, color prior to
elongation 176C. Female inflorescence.--Style color 048B to 047B.
Time of pollen shed.--Descriptor=6 (same as `Daviana`, `Cosford`,
and `Tonda Romana`). Catkin length.--29.5 mm, descriptor=5
(medium). Time of female flowering.--Descriptor=5 (medium). Time of
female flowering compared to male flowering.--Protogyny,
descriptor=1 (earlier). Involucre constriction.--Absent. Involucre
length.--Twice length of nut, descriptor=7. Size of
indentation.--Descriptor=7 (strong). Strength of serration of
indentation.--Descriptor=7 (strong). Pubescence on
husk.--Descriptor=9 (present). Thickness of callus at
base.--Descriptor=5 (medium). Jointing of bracts.--Descriptor=2 (on
one side). Nut description: Length.--About 18.3 mm. Width.--About
19.0 mm. Depth.--About 16.6 mm. Nut shape.--Round. Nut shape index
[(width+depth)/2*length]--0.97. Nut compression index
(width/depth).--1.15. Nut shell color.--164A. Nut weight.--About
2.39 grams. Kernel weight.--About 1.11 grams. Kernel percentage
(kernel weight/nut weight).--About 43.9%. Number of fruits per
cluster.--Two to three. Number of stripes on shell.--Descriptor=5
(medium). Prominence of fruit apex.--Slight, descriptor=3. Size of
fruit pistil.--Very small, descriptor=3. Hairiness of top of
fruit.--Weak, descriptor=3. Curvature of nut basal scar.--Flat.
Double kernels.--Absent. Kernel shape.--Globular. Shape of kernel
in cross-section.--Circular. Lateral groove in kernel.--Absent.
Corkiness of pellicle of kernel.--Descriptor=5 (medium corky). Nut
yield (pounds per tree or per acre).--Total 25.91 kg per tree,
years 3 to 7 (1.sup.st trial) (very high) total 19.67 kg per tree,
years 3 to 7 (2.sup.nd trial). Storability of fruits.--Excellent,
similar to OSU releases and check cultivars. Disease/pest
resistance: Plants of the new Corylus are highly resistant to
eastern filbert blight caused by the fungus Anisogramma anomala
(Peck) E. Muller. Plants of the new Corylus are highly resistant to
bud mites (Phytoptus avellanae Nal.), while plants of `Tonda
Gentile delle Langhe` are highly susceptible, and plants of
`Barcelona` are highly resistant. Temperature tolerance: Plants of
the new Corylus have been observed to tolerate temperatures from
-10 to 38 degrees C. in the field in Corvallis, Oreg.
TABLE-US-00001 TABLE 1 Primers and annealing temperatures for the
20 microsatellite marker loci used to fingerprint `Wepster` and
other hazelnut cultivars. Locus Repeat motif Size T.sub.a n He Ho
A614 (TC).sub.17(CA).sub.10 125-156 60 14 0.85 0.85 NNN(CA).sub.6
A616 (AC).sub.11 136-162 60 13 0.85 0.85 A640
(CT).sub.15(CA).sub.13 354-378 67 11 0.80 0.73 B617 (GA).sub.15
280-298 60 9 0.80 0.78 B619 (TC).sub.21 146-180 60 14 0.88 0.88
B634 (AG).sub.15 218-238 60 9 0.76 0.76 B671
(AG).sub.6NN(GA).sub.17 221-249 60 13 0.86 0.88 B709 (GA).sub.21
219-233 60 16 0.87 0.80 B733 (TC).sub.15 161-183 60 8 0.68 0.68
B749 (TC).sub.12 200-210 60 6 0.60 0.64 B767 (TC).sub.15(AT).sub.7
198-238 60 16 0.87 0.80 B774 (AG).sub.15 195-213 60 8 0.80 0.80
B795 (TC).sub.8Ns(CT).sub.7Ns 296-332 60 12 0.76 0.74
(CT).sub.10Ns(TC).sub.5 C115 (TAA).sub.5(GAA).sub.12 167-226 60 14
0.80 0.80 KG807 (TAAA)AA(TAAA).sub.2 226-248 54 4 0.67 0.78
A(TAAA).sub.2 KG809 (AGG).sub.6 333-345 55 5 0.66 0.64 KG811
(GA).sub.17 240-278 58 12 0.83 0.82 KG827 (CT).sub.13AA(CA).sub.7
264-282 67 9 0.78 0.84 KG830 (CT).sub.14GTATT(CA).sub.8 279-311 67
9 0.79 0.78 Soman-G (AAT).sub.5 193-200 54 3 0.60 0.98 (=856-
MS1-13) Primers 5'-3' Locus PIC r LG (forward) A614 0.84 0.00 6
Hex-TGGCAGAGCTTTGT CAGCTT (SEQ ID NO: 1) A616 0.83 0.00 8
Fam-CACTCATACCGCAA ACTCCA (SEQ ID NO: 3) A640 0.77 0.04 10
F-TGCCTCTGCAGTTAGTC ATCAAATGTAGG (SEQ ID NO: 5) B617 0.78 0.01 8
Fam-TCCGTGTTGAGTATG GACGA (SEQ ID NO: 7) B619 0.87 0.00 3
Fam-AGTCGGCTCCCCT TTTCTC (SEQ ID NO: 9) B634 0.73 0.00 4
Hex-CCTGCATCCAGGACT CATTA (SEQ ID NO: 11) B671 0.84 -0.01 9
Hex-TTGCCAGTGCATACT CTGATG (SEQ ID NO: 13) B709 0.86 0.04 8
Fam-CCACCAACTGTTTC ACACCA (SEQ ID NO: 15) B733 0.63 0.00 7,2
Ned-CACCCTCTTCACCAC CTCAT (SEQ ID NO: 17) B749 0.51 -0.03 1
Hex-GGCTGACAACACAG CAGAAA (SEQ ID NO: 19) B767 0.86 0.04 8
Fam-CCACCAACTGTTTC ACACCA (SEQ ID NO: 21) B774 0.77 0.01 5
Ned-GTTTTGCGAGCTCATT GTCA (SEQ ID NO: 23) B795 0.74 0.01 NA
Fam-GACCCACAAACAAT AACCTATCTC (SEQ ID NO: 25) C115 0.77 0.00 4
Fam-ATTTTCCGCAGAT AATACAGG (SEQ ID NO: 27) KG807 0.60 -0.07 11
AAGCAAGAAAGGGATGGT (SEQ ID NO: 29) KG809 0.60 0.01 4
Hex-AGGCATCAGTTCA TCCAA (SEQ ID NO: 31) KG811 0.81 0.01 2
Ned-AAGGCGGCACTC GCTCAC (SEQ ID NO: 33) KG827 0.75 -0.04 9
Fam-AGAACTCCGACTA ATAATCCTAACCCTTGC (SEQ ID NO: 35) KG830 0.76 0.00
9 Ned-TGGAGGAAGTTTT GAATGGTAGTAGAGGA (SEQ ID NO: 37) Soman-G 0.51
-0.27 NA Hex-TGGCGTTGCAACAT (=856- ATTCTC (SEQ ID NO: 39) MS1-13)
Primers 5'-3' Locus (reverse) Reference A614 R-GCAGTGGAGGATTGCTGACT
Gurcan et al. 2010 (SEQ ID NO: 2) A616 R-ATGGCTTTTGCTTCGTTTTG
Gurcan et al. 2010 (SEQ ID NO: 4) A640 Fam-CGCCATATAATTGGATGC
Gurcan et al. 2010 TTGTTG (SEQ ID NO: 6) B617 R-TGTTTTTGGTGGAGCGATG
Gurcan et al. 2010 (SEQ ID NO: 8) B619 R-GCGATCTGACCTCATTTTTG
Gurcan et al. 2010 (SEQ ID NO: 10) B634 R-GTGCAGAGGTTGCACTCAAA
Gurcan et al. 2010 (SEQ ID NO: 12) B671 R-ACCAGCTCTGGGCTTAACAC
Gurcan et al. 2010 (SEQ ID NO: 14) B709 R-GCGAAATGGAGCTCTTGAAC
Gurcan et al. 2010 (SEQ ID NO: 16) B733 R-CATCCCCTGTTGGAGTTTTC
Gurcan et al. 2010 (SEQ ID NO: 18) B749 R-TCGGCTAGGGTTAGGGTTTT
Gurcan et al. 2010 (SEQ ID NO: 20) B767 R-GCGAAATGGAGCTCTTGAAC
Gurcan et al. 2010 (SEQ ID NO: 22) B774 R-TGTGTGTGGTCTGTAGGCAC
Gurcan et al. 2010 T (SEQ ID NO: 24) B795 R-TGGGCATCATCCAGGTCTA
Gurcan et al. 2010 (SEQ ID NO: 26) C115 GTTTCCAGATCTGCCTCCATAT
Bassil et al. AAT (SEQ ID NO: 28) 2005b, Gokirmak et al. 2009 KG807
FAM-CTTACAGATAAATGGCTC Gurcan and AAA (SEQ ID NO: 30) Mehlenbacher
2010 KG809 F-GGAAGGTGAGAGAAATCAAG Gurcan and T (SEQ ID NO: 32)
Mehlenbacher 2010 KG811 F-GAACAACTGAAGACAGCAAA Gurcan and G (SEQ ID
NO: 34) Mehlenbacher 2010 KG827 GAGGGAGCAAGTCAAAGTTGAG Gurcan and
AAGAAA (SEQ ID NO: 36) Mehlenbacher 2010 KG830 AAAGCAACTCATAGC
Gurcan and TGAAGTCCAATCA (SEQ ID Mehlenbacher 2010 NO: 38) Soman-G
R-GCCATCTTTAG unpublished (=856- AAAGTTCGATACAG MS1-13) (SEQ ID NO:
40) Primer fluorescent tags are FAM, HEX, and NED. Ta: annealing
temperature (.degree. C.) N: number of alleles He: expected
heterozygosity Ho: observed heterozygosity PIC: polymorphism
information content r: estimated null allele frequency LG: linkage
group Reference: for development and characterization
TABLE-US-00002 TABLE 2 Allele sizes in Wepstee and other hazelnut
cultivars at 20 microsatellite loci. Tonda OSU Tonda Gentile Bar-
Locus Wepster Pacifica 440.005 delle Langhe celona A614 135/158
135/150 125/158 125/135 125/132 A616 152/160 150/160 152/160
150/152 144/152 A640 368/374 368/374 355/368 355/368 355/374 B617
293/295 293/293 293/295 285/295 285/289 B619 166/172 166/172
166/166 150/166 158/172 B634 228/228 228/228 228/234 228/228
228/228 B671 239/249 229/239 249/249 239/243 225/229 B709 229/235
229/235 223/229 229/229 227/235 B733 173/175 173/175 173/175
173/175 173/175 B749 207/209 207/209 209/209 207/209 209/209 B767
200/242 200/218 212/242 214/218 214/240 B774 203/207 203/207
203/211 203/211 203/207 B795 333/333 315/333 333/333 315/333
333/333 C115 183/194 174/183 194/216 174/174 174/194 KG807 252/252
228/252 252/252 238/252 238/252 KG809 342/342 339/342 342/348
339/342 339/339 KG811 257/257 245/257 257/267 257/267 261/267 KG827
270/282 270/284 272/282 268/278 282/284 KG830 295/305 291/295
295/305 291/295 291/295 Soman-G 196/200 196/200 196/196 196/200
196/200 Locus Vanillin Dorris York Felix Santiam Gasaway A614
132/158 132/158 124/158 138/143 132/158 143/158 A616 150/150
150/152 144/152 150/152 150/152 150/150 A640 355/368 372/374
363/374 368/372 355/362 362/368 B617 289/295 287/295 287/289
287/287 285/295 291/295 B619 158/172 158/166 158/166 158/166
158/166 172/176 B634 236/236 228/228 228/236 228/236 222/236
222/234 B671 225/243 229/249 243/249 229/237 225/237 237/249 B709
229/229 229/229 229/233 229/233 229/229 229/229 B733 181/185
173/181 173/181 175/181 175/181 175/175 B749 209/209 207/207
209/209 207/207 209/209 207/209 B767 214/238 214/218 236/238
214/214 212/214 214/214 B774 203/211 203/207 203/209 203/213
209/213 203/209 B795 333/333 333/333 333/333 321/333 317/333
317/319 C115 197/216 194/216 197/197 197/216 194/197 216/219 KG807
230/252 242/252 242/252 238/242 242/252 242/252 KG809 348/348
339/348 339/348 339/348 339/342 339/348 KG811 251/261 257/267
257/257 251/267 257/267 257/261 KG827 268/282 272/284 268/272
272/284 272/272 272/282 KG830 291/295 295/297 295/295 293/303
291/295 291/305 Soman- 196/200 196/200 196/200 196/200 196/200
196/196 G
References: Bassil N. V., Botta R., Mehlenbacher S. A. 2005a.
Microsatellite markers in hazelnut: Isolation, characterization and
cross-species amplification. J. Amer. Soc. Hort. Sci. 130:543-549.
Bassil N. V., Botta R., Mehlenbacher S. A. 2005b. Additional
microsatellite markers of the European hazelnut. Acta Hort.
686:105-110. Boccacci P., Akkak A., Bassil N. V., Mehlenbacher S.
A., Botta R. 2005. Characterization and evaluation of
microsatellite loci in European hazelnut (C. avellana) and their
transferability to other Corylus species. Molec. Ecol. Notes
5:934-937. Boccacci P., Akkak, A. and Botta, R. 2006. DNA typing
and genetic relations among European hazelnut (Corylus avellana L.)
cultivars using microsatellite markers. Genome 49:598-611. Gokirmak
T., Mehlenbacher S. A., Bassil N. V. 2009. Characterization of
European hazelnut (Corylus avellana) cultivars using SSR markers.
Genetic Resources and Crop Evolution 56:147-172. Gurcan, K., S. A.
Mehlenbacher and V. Erdogan. 2010a. Genetic diversity in hazelnut
cultivars from Black Sea countries assessed using SSR markers.
Plant Breeding (available on-line doi :10. 1111/j. 1439-0523. 2009.
01753. x). Gurcan, K., S. A. Mehlenbacher, N. V. Bassil, P.
Boccacci, A. Akkak and R. Botta. 2010b. New microsatellite markers
for Corylus avellana from enriched libraries. Tree Genetics and
Genomes (available on-line as DOI 10.1007/s11295-010-0269-y).
Gurcan, K. and S. A. Mehlenbacher. 2010. Development of
microsatellite marker loci for European hazelnut (Corylus avellana
L.) from ISSR fragments. Molecular Breeding (available
on-line).
SEQUENCE LISTINGS
1
40120DNAArtificial SequenceSynthetic polynucleotide 1tggcagagct
ttgtcagctt 20220DNAArtificial SequenceSynthetic polynucleotide
2gcagtggagg attgctgact 20320DNAArtificial SequenceSynthetic
polynucleotide 3cactcatacc gcaaactcca 20420DNAArtificial
SequenceSynthetic polynucleotide 4atggcttttg cttcgttttg
20529DNAArtificial SequenceSynthetic polynucleotide 5tgcctctgca
gttagtcatc aaatgtagg 29624DNAArtificial SequenceSynthetic
polynucleotide 6cgccatataa ttggatgctt gttg 24720DNAArtificial
SequenceSynthetic polynucleotide 7tccgtgttga gtatggacga
20819DNAArtificial SequenceSynthetic polynucleotide 8tgtttttggt
ggagcgatg 19919DNAArtificial SequenceSynthetic polynucleotide
9agtcggctcc ccttttctc 191020DNAArtificial SequenceSynthetic
polynucleotide 10gcgatctgac ctcatttttg 201120DNAArtificial
SequenceSynthetic polynucleotide 11cctgcatcca ggactcatta
201220DNAArtificial SequenceSynthetic polynucleotide 12gtgcagaggt
tgcactcaaa 201321DNAArtificial SequenceSynthetic polynucleotide
13ttgccagtgc atactctgat g 211420DNAArtificial SequenceSynthetic
polynucleotide 14accagctctg ggcttaacac 201520DNAArtificial
SequenceSynthetic polynucleotide 15ccaccaactg tttcacacca
201620DNAArtificial SequenceSynthetic polynucleotide 16gcgaaatgga
gctcttgaac 201720DNAArtificial SequenceSynthetic polynucleotide
17caccctcttc accacctcat 201820DNAArtificial SequenceSynthetic
polynucleotide 18catcccctgt tggagttttc 201920DNAArtificial
SequenceSynthetic polynucleotide 19ggctgacaac acagcagaaa
202020DNAArtificial SequenceSynthetic polynucleotide 20tcggctaggg
ttagggtttt 202120DNAArtificial SequenceSynthetic polynucleotide
21ccaccaactg tttcacacca 202220DNAArtificial SequenceSynthetic
polynucleotide 22gcgaaatgga gctcttgaac 202320DNAArtificial
SequenceSynthetic polynucleotide 23gttttgcgag ctcattgtca
202421DNAArtificial SequenceSynthetic polynucleotide 24tgtgtgtggt
ctgtaggcac t 212524DNAArtificial SequenceSynthetic polynucleotide
25gacccacaaa caataaccta tctc 242619DNAArtificial SequenceSynthetic
polynucleotide 26tgggcatcat ccaggtcta 192721DNAArtificial
SequenceSynthetic polynucleotide 27attttccgca gataatacag g
212825DNAArtificial SequenceSynthetic polynucleotide 28gtttccagat
ctgcctccat ataat 252918DNAArtificial SequenceSynthetic
polynucleotide 29aagcaagaaa gggatggt 183021DNAArtificial
SequenceSynthetic polynucleotide 30cttacagata aatggctcaa a
213118DNAArtificial SequenceSynthetic polynucleotide 31aggcatcagt
tcatccaa 183221DNAArtificial SequenceSynthetic polynucleotide
32ggaaggtgag agaaatcaag t 213318DNAArtificial SequenceSynthetic
polynucleotide 33aaggcggcac tcgctcac 183421DNAArtificial
SequenceSynthetic polynucleotide 34gaacaactga agacagcaaa g
213530DNAArtificial SequenceSynthetic polynucleotide 35agaactccga
ctaataatcc taacccttgc 303628DNAArtificial SequenceSynthetic
polynucleotide 36gagggagcaa gtcaaagttg agaagaaa 283729DNAArtificial
SequenceSynthetic polynucleotide 37tggaggaagt tttgaatggt agtagagga
293828DNAArtificial SequenceSynthetic polynucleotide 38aaagcaactc
atagctgaag tccaatca 283920DNAArtificial SequenceSynthetic
polynucleotide 39tggcgttgca acatattctc 204025DNAArtificial
SequenceSynthetic polynucleotide 40gccatcttta gaaagttcga tacag
25
* * * * *