U.S. patent number 3,923,460 [Application Number 05/523,091] was granted by the patent office on 1975-12-02 for dual ion chromatograph using parallel columns for ionic analysis.
This patent grant is currently assigned to The Dow Chemical Company. Invention is credited to Donald Clarence Benefiel, Albert Ross Parrott.
United States Patent |
3,923,460 |
Parrott , et al. |
December 2, 1975 |
Dual ion chromatograph using parallel columns for ionic
analysis
Abstract
Integrated system for the concurrent quantitative analysis,
using ion exchange resin, of chromatographically separable cations
and anions contained in a single measured portion of aqueous sample
solution includes an eluant water reservoir, a sample injection
valve, a pump delivering water from the reservoir to the sample
injection valve, and thence to a first stream splitter feeding two
parallel ion exchange columns. One column is charged with anion
exchange resin and the other with cation exchange resin, preferably
with differing bed depths but in any event selected in nature and
geometry to achieve differing elution times for each ion present
and being determined, and ordinarily to achieve elution of all the
ions of one sign before elution of the ions of the opposite sign,
e.g., all the anions before the cations. The effluent from each
column is joined through a second stream splitter and the resulting
single stream is directed to a conductivity cell with associated
readout means. The effluent from the conductivity cell may be
discarded but is ordinarily passed through clean-up resin bed means
and cycled back to the eluant water reservoir. Preferably means are
provided for (1) substituting columns with different resins and bed
depths for specific analyses, (2) occasionally flushing the sample
solution delivery system with bactericide, or (3) running known
standard solution.
Inventors: |
Parrott; Albert Ross (Lake
Jackson, TX), Benefiel; Donald Clarence (Lake Jackson,
TX) |
Assignee: |
The Dow Chemical Company
(Midland, MI)
|
Family
ID: |
24083622 |
Appl.
No.: |
05/523,091 |
Filed: |
November 12, 1974 |
Current U.S.
Class: |
436/149; 210/264;
422/70; 73/61.53; 210/659; 436/161 |
Current CPC
Class: |
G01N
30/466 (20130101); G01N 30/96 (20130101); G01N
2030/202 (20130101); G01N 2030/207 (20130101); G01N
2030/402 (20130101); G01N 2030/628 (20130101); G01N
2030/965 (20130101) |
Current International
Class: |
G01N
30/96 (20060101); G01N 30/46 (20060101); G01N
30/00 (20060101); G01N 30/60 (20060101); G01N
027/08 (); G01N 031/04 (); G01N 031/08 () |
Field of
Search: |
;23/23R,253R ;73/61.1C
;210/25,31C,264,37,38 ;127/9 |
References Cited
[Referenced By]
U.S. Patent Documents
Primary Examiner: Wolk; Morris O.
Assistant Examiner: Marantz; Sidney
Attorney, Agent or Firm: Schilling; Edward E.
Claims
We claim:
1. Chromatographic apparatus for the concurrent quantitative
determination of chromatographically separable cations and anions
in aqueous solution from a single measured portion of sample
solution which comprises:
first and second tubular columns each adapted to hold a charge of
ion exchange resin;
first and second stream splitters, a sample selection valve, a
pump, a water reservoir and a conductivity cell; and
liquid conduit means sequentially connecting, in series, the water
reservoir, the pump, the same selection valve and the first stream
splitter, then, in parallel, between the first stream splitter and
the second stream splitter, the first and second tubular columns,
and finally, in series, the second stream splitter and the
conductivity cell.
2. The apparatus as in claim 1 wherein the second tubular column is
at least 25 percent longer than the first tubular column.
3. The apparatus as in claim 1 wherein the second tubular column is
at least 50 percent longer than the first tubular column.
4. The apparatus as in claim 1 wherein the first tubular column
contains a cation exchange resin and the second tubular column
contains an anion exchange resin, the cation exchange resin being
in the hydrogen form and the anion exchange resin being in the
hydroxide form.
5. The apparatus as in claim 1, including, in addition, at least
one additional tubular column and additional liquid conduit means
connecting in series the conductivity cell, the at least one
additional tubular column and the water reservoir thereby providing
a closed loop.
6. The apparatus as in claim 5 wherein the at least one additional
tubular column is charged with a double bed of ion exchange resin
combination adapted to substantially demineralize aqueous solution
effluent from the conductivity cell passed therethrough the double
bed.
7. The method of chromatographically quantitatively determining
concurrently cationic species as well as anionic species in a given
portion of aqueous sample solution with a single detector, the
cations and anions being chromatographically separable from ion
species of like charge, which comprises:
introducing and blending a known quantity of said aqueous sample
solution into a flowing stream of water;
dividing the blend of sample and water stream into a first and
second stream;
directing the first stream through a first tubular column
containing a charge of anion exchange resin in the hydroxide form
and chromatographically separating said cationic species therein
and eluting the separated cationic species therefrom;
simultaneously directing the second stream through a second tubular
column containing a charge of cation exchange resin in the hydrogen
form and chromatographically separating said anionic species
therein and eluting the separated anionic species therefrom;
and
conjoining the effluent streams exiting from the first and second
tubular columns and passing the conjoined streams through a
conductivity cell capable of detecting each of the
chromatographically separated cationic species and anionic species,
the relative depths of the anion and cation exchange resin beds and
the relative flow rates of the first and second streams being
cooperatively preselected to avoid interference between ionic
species being detected by the conductivity cell.
8. The method as in claim 7 wherein the number of each of the
cationic species and anionic species present, respectively does not
exceed six.
9. The method as in claim 7 wherein the number of each of the
cationic species and anionic species present, respectively, does
not exceed three.
10. The method as in claim 7 wherein the ion exchange resin bed
depths and the relative flow rates are so selected that the each of
the anionic species present elute from the ion exchange resin in
the second tubular column and reach the conductivity cell before
any of the cationic species elute from the ion exchange resin in
the first tubular column and reach the conductivity cell.
11. The method as in claim 7 wherein the ion exchange resin bed in
the first tubular column has a depth at least 1.25 as great as the
ion exchange resin bed in the second tubular column.
12. The method as in claim 7 wherein the ion exchange resin bed in
the first tubular column has a depth at least 1.5 as great as the
ion exchange resin bed in the second tubular column.
Description
CROSS-REFERENCE TO RELATED APPLICATION
In a copending application of T. S. Stevens and W. H. Parth, Ser.
No. 386,259, filed Aug. 6, 1973, there is described an integrated
system for quantitative analysis, using ion exchange resins, of the
number of equivalents of ionic species present by converting all
the species to a given ion pair and measuring the concentration of
such pair with a conductivity cell.
BACKGROUND OF THE INVENTION
1. Field of the Invention
The invention relates to an integrated system for the quantitative
analysis of an aqueous solution containing a limited number of
separable ion species, the determination of all cations and anions
being determined being carried out on a single injected sample
portion and the anions and cations being determined
concurrently.
2. Description of the Prior Art
There is a constant and ever increasing demand for a rapid,
inexpensive method of analysis of large numbers of samples of
aqueous solutions containing a relatively small number of species
of ion pairs dissolved therein. There is also a great need for a
simple automatable apparatus for carrying out such analyses as well
as for analysis of process streams for a small number of specified
ion pairs or for a given ion in the presence of a small number of
ion pairs, such as is present, theoretically, with up to six cation
species and up to six anion species represented in the solution, in
readily detectable amounts.
The determinations of dissolved ions in such aqueous solutions have
generally been carried out using old classical, slow, and
reinatively costly methods. Even where ion exchange separations,
chromatographically or by exchange, have been utilized, the
fractions obtained have generally been analyzed by classical
methods which usually require a different test and/or a different
instrument for each species to be determined, particularly with
respect to the anions to be determined. Therefore, a new approach
with new apparatus appears to be needed.
SUMMARY OF THE INVENTION
Concurrent quantitative determination of chromatographically
separable cations and anions in aqueous solution from a single
measured portion of sample solution is readily carried out using a
system which includes: first and second tubular columns each
adapted to hold a charge of ion exchange resin; first and second
stream splitters, a sample selection valve, a pump, a water
reservoir and a conductivity cell having associated readout means;
and liquid conduit means sequentially connecting, in series, the
water reservoir, the pump, the sample selection valve and the first
stream splitter, then in parallel, between the first stream
splitter and the second stream splitter, the first and second
tubular columns, and finally, in series, the second stream splitter
and the conductivity cell. In using the system, one column is
charged with an anion exchange resin and cations are
chromatographed thereon while the other column is charged with a
cation exchange resin and anions are chromatographed thereon,
concurrently, that is, substantially simultaneously with the cation
separation and from the same sample portion divided by the stream
splitter. The column or bed geometry is selected to facilitate
elution of the separated ions at discretely different times so that
quantitative detection with a conductivity cell can be
accomplished. Generally, a clean-up resin bed means is employed
following the conductivity cell, and the eluant water is cycled
back to the water reservoir in a closed loop system which is
readily automated, in which case it is generally desirable to
include means for running a standard solution periodically and also
flushing the sample selection valve and associated parts
occasionally with a bactericide solution.
BRIEF DESCRIPTION OF THE DRAWINGS
The single FIGURE of the drawing is a schematic representation of
chromatographic apparatus for the concurrent quantitative
determination of chromatographically separable cations and anions
in aqueous solution from a single measured portion of sample
solution per concurrent determination, the apparatus including dual
columns connected in parallel for the substantially simultaneous
separation of anions on one column and cations on the other column,
according to the invention.
FURTHER DESCRIPTION OF THE INVENTION
An embodiment of the present chromatographic apparatus, or dual ion
chromatograph, for the concurrent quantitative analysis of
chromatographically separable cations and anions in a single sample
portion, as shown in the drawing, includes a water reservoir 10,
normally containing a substantial quantity of water 11 which is
used as eluant, a sample selection valve 12 ordinarily in the form
of a conventional sample injection valve, a pump 13 for conveying
eluant water 11 to the sample selection valve 12, a first stream
splitter 14 receiving eluant water and any sample admixed therewith
in the sample selection valve 12, and dividing the stream and
directing the divided parts to the first tubular column 15, which
is charged with cation exchange resin when ready for use, and the
second tubular column 16, which is charged with anion exchange
resin when ready for use, a second stream splitter 17 which here
serves as a stream combining means on receiving the effluent from
both of the tubular columns 15, 16, a conductivity cell 18 with
associated readout means 19, receiving the conjoined column
effluents, clean-up resin bed means indicated generally by the
numeral 20 and taking the form here of two columns 21, 22 in series
and normally charged with a bed of cation exchange resin in the
hydrogen form and a bed of anion exchange resin in the hydroxide
form in the respective columns for deionizing the effluent water,
and liquid conduit means 23 for connecting the above-described
parts in the sequence described, as well as connecting the clean-up
resin bed means 20 to the water reservoir 10.
The first tubular column 15, when ready for use, is charged with a
cation exchange resin in the hydrogen form, while the second
tubular column 16 is charged with an anion exchange resin in the
hydroxide form. Anions must become separated chromatographically
while traversing the first column 15, else a smaller sample or a
deeper bed of resin must be employed or both, if success is to be
achieved, that is, the ion species separated, so that the
conductivity cell detects separated bands or groups of ions. Or, a
particular ion exchange resin may be selected that holds the ions
of interest in a differential manner. Similarly, cations must
become separated chromatographically while traversing the second
column 16 containing anion exchange resin.
In order to avoid the elution of cations and anions simultaneously,
that is, from both columns at once, column 16 is selected from or
made up as a longer column than column 15, or at least the charge
of resin therein is deeper in column 16. Generally separate
issuance of anions prior to the issuance of cations is assured upon
making the resin bed or column 16 at least 25 percent greater in
depth, and preferably at least 50 percent greater in depth, than
the resin bed depth in column 15.
While the stream splitter 14 will ordinarily be one that divides
the stream evenly between the two columns, by adjusting the split
between the two columns, and therefore the amount of eluant water
applied to the columns respectively, further adjustments are
possible in the relative elution times from the two columns as will
be appreciated by those skilled in the art.
The sample selection valve 12 is of the general type commonly used
for chromatographic analyses and typically is provided with a
measuring bore in the valve plug of known volume or a pair of ports
to the valve body are connected by a tubing loop of known volume,
and the valve is provided with bypass means for continuously
directing eluant water through the valve to the stream splitter 14
and through the system while sample solution, or standard solution,
as the case may be, flows continuously through the measuring bore
or the tubing loop and discharges continuously to a waste
stream.
In the case of individual samples under analysis a syringe or pipet
may be used to fill the measuring loop, or the sample, as measured
by the syringe, is simply injected through a septum into the eluant
stream. In the case of a sample stream, a by-pass line, not shown,
can be used to bring sample to the sample intake of the sample
selection valve 12, and through the measuring section described
above.
Wherein the measuring loop is used, the valve is manipulated to
bring the sample-filled volume into series with the eluant stream
of water constantly passing through a portion of the valve, and the
selected sample portion of known volume is swept on into the
system. If the apparatus is automated, a computer-controller, not
shown, will cause the selection valve to be actuated to bring the
sample measuring loop to be swept out by eluant water and will
coordinate the readout of the conductivity cell therewith.
The sample selection valve 12 should provide for measuring and
injecting a predetermined sample size in the range of about 2 to
about 1000 microliters. Typically chosen sample sizes adequate for
detecting most ionic materials and small enough to avoid
unnecessary exhaustion of the ion exchange resins used are in the
range of about 5 to about 50 microliters.
The sample selection valve 12 is preferably either a rotary valve
such as the Model R 6031 SVA-K valve supplied by Chromatronix,
Inc., or a slide valve such as the Model CSVA-K valve from the same
supplier, or the equivalent of either. When using remotely actuated
valves in an automated system the valves must each be provided with
appropriate actuators.
The columns used to house the ion exchange resins, viz, columns 15
and 16, are best selected from glass or metal columns now readily
available commercially and having the proper fittings to be easily
connected into a system. While larger columns may generally be
used, if desired, such as those having 25 to 50 mm. inside diameter
(ID), the smaller columns utilizing smaller resin beds better serve
the purposes of obtaining rapid, sharp analytical separations and
the preferred column sizes are in the range of about 1 to 10 mm. ID
and from about 5 to 1000 cm. length, provided the two columns are
relatively proportioned, one to the other as described above, but
more generally selected sizes are 2.8 mm. or 9 mm. ID and the first
column has a length of about 20 to 30 cm. and the second column is
proportionately longer to provide for the requisite deeper resin
bed needed, although both columns may be the same length and the
second column filled with a greater depth of ion exchange resin,
i.e., with a larger charge of resin.
The resin charged to the columns 15 and 16 should be high specific
exchange capacity ion exchange resins. The ion exchange resins used
for chromatographic separations herein gradually become exhausted
according to their specific exchange capacities, amounts of resin
used, and the number of equivalents of ion species in the samples
exchanging with the ions at the active sites of the resins.
Preferably, for the sake of economy of operator attention the
number of equivalents in selected sizes of samples and the total
exchange capacity of the ion exchange resins used permit the
analysis of at least about 500 and more preferably 5000 or more
samples before the charge of resin in a given column becomes
exhausted.
The ion exchange resins usable in the present method and apparatus
are typically polystyrene or modified polystyrene copolymers
cross-linked, e.g., with divinylbenzene, and carrying nuclear
groups, the latter providing active exchange sites. The cation
exchange resins carry nuclear sulfonic acid or sulfonate groups
along the polymer chain. The strong base anion exchange resins
carry nuclear chloromethyl groups which have been quaternized.
For further information on ion exchange theory, processes and
resins synthesis reference is made to the monograph "Dowex: Ion
Exchange" 3rd Ed. 1964, published by The Dow Chemical Company,
Midland, Mich., and the two-volume work "Ion Exchange" Ed. by Jacob
A. Marinski and published by Marcel Dekker Inc., New York, 1966.
Chapter 6, Vol. 2, of "Ion Exchange" is devoted to a description of
synthesis of ion exchange resins of various types usable
herein.
The dimensions of the clean up resin bed columns 21, 22 used to
house the clean up resins beds are not critical as analytical
separations are not carried out therein. Most any geometry suffices
so long as the ion exchange resin placed therein has a total
exchange capacity sufficient to deionize the eluant water effluent
from the conductivity cell for a very large number of samples,
preferably handling at least as many samples as the ion exchange
resins in columns 15 and 16. Usually columns with the same bed
volumes as that of column 15 suffices.
Instead of using two columns in series as shown in the drawing the
clean up resin bed means 20 may take the form of a single column
which will be charged with either a two layer bed or a mixed bed
resin for deionization of the eluant water. In carrying out
analyses according to the invention, both anions and cations in the
effluent from the conductivity cell will need to be exchanged for
hydroxide ions and hydrogen ions respectively in the clean up bed
resins. The quality of the deionized water should be such as to
give a very low base line reading, e.g., about 2 micromho/cm, when
passed through the conductivity cell.
The conductivity cell employed is selected from most any of the
conventional commercially available models regularly used in
conductimetric detection chromatography.
An example of a suitable conductivity cell is Model MCC 75
available from Chromatromix, Inc. A suitable conductivity meter for
use therewith is Model CM 1A from the same supplier. Most any meter
selected is preferably modified, for the present purposes, to
reduce zero suppression.
In addition, the apparatus may include provision (not shown) for
bringing sample solution from a process stream to the sample
selection valve 12, and filtering the solution, if desirable.
To assure accurate results by making corrections from time to time
for such changes as instrument component drift, it is highly
desirable and usually considered essential to supply a known or
standard solution to the analysis apparatus on an intermittent
basis and mutually exclusively to the supplying of sample solution.
Accordingly, standard solution stored in a reservoir 24 is supplied
as by gravity through a three-port selector valve 25 which, when
appropriately set, delivers standard solution to the sample
injection valve 12 with the aid of pump 26, if necessary, while
stopping the flow of sample solution for the duration of the
running of the standard solution to the sample injection valve
12.
To prevent the growth of bacteria, algae or anaerobic organisms in
the system, it may be found desirable, especially in the sampling
and analysis of some solutions using automated apparatus, to
provide means for periodically flushing the three-port valve 25,
the pump 26 and parts of the sample injection valve 12 and the
liquid conduit means interconnecting the same with a bactericide
solution. Solutions such as aqueous sulfuric acid, or inhibited
hydrochloric acid, having a concentration of e.g., 4 normal, or an
aqueous solution of most any of the organic chemical bactericidal,
algaecidal or slimicidal compounds used in keeping cooling towers,
sampling lines, and the like free from organisms, may be used. Such
bactericide solution is stored in a container 27 which serves as a
reservoir and, on appropriately setting the selector valve 25, the
bactericide solution flows to the pump 26 and into the sample
selection valve 12, at times when sample and also standard
solutions are mutually exclusively shut off, relative to the
bactericide solution. Since the bactericide would be deleterious to
the resin beds it is excluded therefrom by closing off access to
the columns at the sample injection valve 12; all of the
bactericide solution exits through a waste port in the sample
injection valve 12.
While the columns 15, 16 and the resin beds therein may be readily
replaced when the resin is exhausted, it may also be desirable to
backwash and regenerate each bed in place, utilizing multi-port
valves 28 and 29 in working with column 15, and multi-port valves
30 and 31 in connection with column 16.
Candidate sample solutions analyzable by the present method and
apparatus therefor are aqueous solutions, as indicated herein
above, containing ion species that are resolvable on an ion
exchange resin column serving as a chromatographic column, a cation
exchange resin column for the separation of anions and an anion
exchange resin column for the separation of cations, and the column
geometries varying sufficiently that the individual ions elute at
different times, usually all of the anions first. It is not
essential that all of the ions of a given balance sign elute first
so long as the peaks in the output of the conductivity cell are
identifiable.
Resolvability of all of the ions present is most likely to be
achieved easily if the number of cations and the number of anions
in each is no more than six and even more so if each is no more
than three. Expecially likely to be resolvable is a solution
containing the conjugate bases of a weak and a strong acid and the
conjugate acids of a weak and a strong base, or either alone.
Traces of other ions may be present providing they elute at
different times than the ions under determination or providing they
do not significantly alter the conductivity cell output at peaks
for the ions under determination. The output is not altered
significantly if it does not change the readout of apparent ion
concentration by an increment greater than the limits of accuracy
required of the analyst. Typically, this limit might be 1 to 5
percent of the actual ion concentration.
The following example serves to illustrate and not to limit the
scope of the invention.
EXAMPLE
Using substantially the apparatus represented schematically in the
drawing and having a cation exchange resin column 9 mm ID and 250
mm in length and charged with a commercial cation exchange resin,
Dowex 50W X16, 200-400 mesh (37-74 microns) in the hydrogen form
and an anion exchange resin column 9 mm ID and 500 mm in length and
charged with a commercial anion exchange resin, Dowex 1 X8, 200-400
mesh (37-74 microns), in the hydroxide form, a solution containing
sodium chloride, sodium carbonate, and ammonium hydroxide that was
too concentrated to analyze without dilution was diluted 1:25 with
deionized water and 40 microliters of the diluted sample was
introduced into the system through the sample selection valve. With
eluant water flowing at the rate of 345 ml per hour the readout of
the conductivity cell on a meter with stripchart recorder appeared
as well separated peaks at 1.5, 3.0, 4.5, and 7.0 minutes for,
respectively, chloride ion, carbonate ion, sodium ion, and ammonium
ion. Identification was confirmed by running known standardized
mixtures from which it was also possible to compute the sample
composition, from conductivity cell response, to be, by weight,
13.0 percent sodium chloride, 12.5 percent sodium carbonate, and
13.0 percent ammonia. Molar concentrations are as follows:
Diluted Sample Original Sample moles/liter moles/liter
______________________________________ Chloride 0.0089 0.222
Carbonate 0.0047 0.118 Sodium 0.0183 0.458 Ammonium 0.0148 0.371
______________________________________
* * * * *