U.S. patent number 3,898,457 [Application Number 05/411,839] was granted by the patent office on 1975-08-05 for methods and apparatus for handling flexible liquid sample containers for scintillation spectrometry.
This patent grant is currently assigned to Packard Instrument Company, Inc.. Invention is credited to Lyle E. Packard, Ariel G. Schrodt.
United States Patent |
3,898,457 |
Packard , et al. |
August 5, 1975 |
Methods and apparatus for handling flexible liquid sample
containers for scintillation spectrometry
Abstract
Methods and apparatus for handling flexible sample containers
used for liquid scintillation spectrometry analysis where detector
photomultiplier tubes are moved axially together "squeezing" the
flexible container therebetween in the counting station to achieve
close optical coupling. The sample containers may be made up of
layered, flexible, light-transmissive, polyester film wherein the
sample container portion is defined by joining the film layers. The
flexible container may be individual units or a continuous strip
and may include provision for identification data or records by way
of a magnetic strip or punch character designation. Optimum
counting efficiency is achieved with round flask-like
configurations where the flexible container conforms to the shape
of the photomultiplier tubes used therewith.
Inventors: |
Packard; Lyle E. (Chicago,
IL), Schrodt; Ariel G. (Wilmette, IL) |
Assignee: |
Packard Instrument Company,
Inc. (Downers Grove, IL)
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Family
ID: |
26888167 |
Appl.
No.: |
05/411,839 |
Filed: |
November 1, 1973 |
Related U.S. Patent Documents
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Application
Number |
Filing Date |
Patent Number |
Issue Date |
|
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192543 |
Oct 26, 1971 |
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Current U.S.
Class: |
250/328; 250/303;
250/367; 250/494.1; 422/68.1; 422/71; 422/82.05; 422/944 |
Current CPC
Class: |
C08B
11/08 (20130101); C08B 17/06 (20130101); G01T
7/08 (20130101); B01L 3/505 (20130101) |
Current International
Class: |
C08B
11/08 (20060101); C08B 17/00 (20060101); C08B
17/06 (20060101); C08B 11/00 (20060101); B01L
3/00 (20060101); G01T 7/00 (20060101); G01T
7/08 (20060101); G01t 001/204 () |
Field of
Search: |
;23/253R
;250/303,304,252,366,367,494,496,497,302 |
References Cited
[Referenced By]
U.S. Patent Documents
Primary Examiner: Dixon; Harold A.
Attorney, Agent or Firm: Wolfe, Hubbard, Leydig, Voit &
Osann, Ltd.
Parent Case Text
This is a continuation of application Ser. No. 192543 filed Oct.
26, 1971 and now abandoned.
Claims
We claim as our invention:
1. In an apparatus for spectrally analyzing test samples containing
one or more radioactive isotopes disposed in a liquid scintillator
and adapted to handle a plurality of sample containers, the
combination comprising, housing means including a source of said
sample containers, at least one photomultiplier detection device
and storage means for receiving said containers, said
photomultiplier detection device being disposed adjacent a linear
path extending between said sample source and said sample storage
means, a light-tight counting station between said sample source
and said sample storage means and disposed on said path, means for
conveying said sample containers in a continuous strip from said
source along said path and individually into the counting station
and, thereafter, into said storage means, means for axially
shifting said photomultiplier detection device toward and away from
the counting station, and a second photomultiplier detection device
in a longitudinally spaced apart relation to said other
photomultiplier detection device.
2. Apparatus as claimed in claim 1 wherein said conveying means
simultaneously transfers adjacent ones of said containers from said
source to said counting station and said storage means.
3. In an apparatus for spectrally analyzing test samples containing
one or more radioactive isotopes disposed in a liquid scintillator
and adapted to handle flexible sample containers formed by joining
layered, flexible, light-transmissive, polyester film to define a
bag portion therein, the combination comprising, housing means
including a source of said flexible sample containers, at least one
photomultiplier detection device and storage means for receiving
said flexible containers, said photomultiplier detection device
being disposed between said sample source and said sample storage
means, a light-tight counting station adjacent said photomultiplier
detection device, means for axially shifting said photomultiplier
detection device toward and away from said counting station so that
said flexible containers are squeezed against said photomultiplier
detection device during said spectrally analyzing, means for
conveying said flexible sample containers from said source into the
counting station and thereafter into said storage means, and said
flexible containers are disposed on a continuous strip and said
conveying means simultaneously transfers adjacent ones of said
flexible containers from said source to said counting station and
said storage means.
4. A method of spectrally analyzing test samples containing one or
more radioactive isotopes disposed in a liquid scintillator and
confined in a sample container, comprising the steps of, storing a
plurality of said containers at one side of a counting station
having at least one photomultiplier detection device adjacent said
counting station, shifting said containers in a continuous strip
from said source to the counting station, locating individual ones
of said containers of the strip in the light-tight counting
station, counting the scintillations produced within the sample
container in the counting station, shifting the counted sample
container out of said counting station to a storage means at the
opposite side of said counting station, said sample container being
one of a plurality of flexible containers formed of layered,
light-transmissive, polyester film, spacing said photomultiplier
detection device during said counting at a distance from a surface
adjacent the counting station which is less than the thickness of
the filled sample containers such that said containers when shifted
into the counting station are squeezed between said photomultiplier
detection device and said surface so that the face of the container
in the counting station conforms to the face of the photomultiplier
detection device.
5. A method as claimed in claim 4 wherein said surface comprises
another photomultiplier detection device.
6. A method as claimed in claim 4 wherein said container is formed
by joining at least a pair of layers of flexible light-transmissive
polyester film and conforms in geometry to the contacting surface
of said photomultiplier detection device.
7. A method as claimed in claim 4 wherein said spacing step
includes axially shifting said photomultiplier detection device
toward said sample container in the counting station until
sufficient pressure is applied to the face of the container so that
it conforms to the face of the photomultiplier detection device,
and
axially shifting said photomultiplier detection device away from
the container after completion of the count.
8. A method as claimed in claim 4 wherein said flexible containers
include at least one face conforming in geometry to the surface of
said photomultiplier detection device.
Description
RELATED APPLICATIONS
Lyle E. Packard Application Ser. No. 630,892 filed Apr. 19, 1967,
assigned to the assignee of the present application, and now U.S.
Pat. No. 3,688,120.
DESCRIPTION OF THE INVENTION
This invention relates generally to methods and apparatus for
handling sample containers for liquid scintillation spectral
analysis of test samples containing one or more radioactive
isotopes disposed in a liquid scintillator. More particularly, the
invention relates methods and apparatus for handling the flexible
sample containers for liquid scintillation detection operations in
a wholly automatic manner, yet which permit maintainance of high
counting efficiency and quantitative accuracy of result.
Background of the Invention
In conventional liquid scintillation counting, a radioactive
nuclide dissolved or dispersed in a scintillator liquid is
contained in a thin plastic or glass sample vial. Liquid
scintillation spectrometers, or apparatus designed to provide
spectral analysis of the test samples contained in such vials have
been successfully utilized now for several years. One system which
has found great acceptance today by people employing such
sophisticated equipment is shown in U.S. Pat. No. 3,188,468 to L.
E. Packard, assigned to the assignee of the present invention. Such
apparatus is loaded with trays holding sample vials containing the
scintillator and isotope, and the vials are manipulated seriatim
into and out of a photomultiplier detection mechanism.
One of the important determinants of the quantitative accuracy of
scintillation detectors is the efficiency with which the
photomultiplier tubes can detect the scintillations. Some light is
inevitably lost at the surfaces of the sample vial and of the
photomultipliers, and as a consequence the efficiency of light
detection is reduced. In other words, the closeness of optical
coupling is one of the factors entering into an efficient
scintillation counting apparatus.
It has been recognized that close optical coupling is desirable for
high counting efficiency. In the aforementioned Packard U.S. Pat.
No. 3,188,468 close optical coupling is achieved with the use of
"light pipes" which are blocks of plastic having a central
vial-receiving well, and with all external surfaces other than
those facing the photomultipliers coated with a reflective
material. Other arrangements for achieving the close optical
coupling have been suggested such as vials having flexible walls
adjacent the sides facing the photomultipliers and which walls
conform to the surface of the photomultiplier tubes so as to remove
air gaps. One such arrangement is illustrated in U.S. Pat. No.
2,750,514. Also, close coupling has been achieved with the use of a
liquid having an index of refraction similar to that of the glass
or plastic vial.
Another technique employed for improving counting efficiency has
been described in an article by Gopi N. Gupta, "Simplified
Solid-State Scintillation Counting on Glass Microfilm Medium in
Plastic Bag for Hydrogen-3, Carbon-14 and Chlorine-36 in Biological
and Organic Material" which was published in Anal. Chem., 39, 1911
(1967), The author used a heat sealed polyester film bag to contain
a radionuclide-impregnated fiberglass sheet, the bag in turn being
placed within a conventional plastic counting vial.
Accordingly, it is a general aim of the present invention to
provide improved flexible sample containers handling methods and
mechanisms for liquid scintillation spectrometry characterized in
that the containers may be constructed at relatively low cost and
can be easily filled, handled and subjected to spectral analysis
with optimum counting efficiency.
A more detailed objective of the invention is the provision of a
flexible sample container which is capable of being mechanically
handled, and eliminates the need for extraneous vials, external
light pipes or the like and yet is conformable with the surface(s)
of the photomultiplier tube(s) to optimize counting geometry.
It is still another object of the present invention to provide a
flexible sample container which may be formed in a continuous strip
wherein a plurality of samples may be stored and handled in a
compact assembly.
Yet another objective of the invention is the provision of
handling, changing and transfer mechanisms which will operate with
a continuous strip of flexible sample containers of the foregoing
type.
DESCRIPTION OF DRAWINGS
Other objects and advantages of the invention will become apparent
upon reading the following detailed description and upon reference
to the drawings in which:
FIG. 1 is a front sectional view of an exemplary radioactive sample
handling and measuring apparatus, housed in a suitable cabinet and
depicting one form of handling of flexible containers of the
present invention;
FIG. 2 is an enlarged view of one form of a portion of a strip of
flexible containers of the present invention used in conjunction
with the apparatus shown in FIG. 1;
FIG. 3 is an enlarged view of a filled flexible container of the
type shown in FIG. 2;
FIG. 4 is an enlarged, fragmentary view of another form of a strip
of flexible containers incorporating the features of data recording
and identification of the present invention;
FIG. 5 is an enlarged fragmentary view of a flexible container
incorporating another form of data recording and
identification;
FIG. 6 shows another version of a strip of flexible containers,
here illustrating individual containers attached to conveyor
strips;
FIG. 7 is an enlarged fragmentary view of a further form of a strip
of flexible containers, here depicting sprocket and locating
aperatures provided along the marginal edges thereof;
FIG. 8 is a diagramatic view illustrating a strip of flexible
containers moving in position between spaced apart photomultiplier
tubes of the detector apparatus for introducing a sample container
therebetween; and,
FIG. 9 is a diagramatic view similar to FIG. 8, here depicting the
position of the flexible container with respect to the
photomultiplier tubes during counting.
While the invention is susceptible of various modifications and
alternative forms, specific embodiments thereof have been shown by
way of example in the drawings and will herein be described in
detail. It should be understood, however, that it is not intended
to limit the invention to the particular forms disclosed, but, on
the contrary, the intention is to cover all modifications,
equivalents and alternatives falling within the spirit and scope of
the invention as expressed in the appended claims.
General Apparatus Organization
Turning first to FIG. 1, there is shown a liquid scintillation
apparatus, indicated generally at 10, which houses a scintillation
detector mechanism or apparatus 12. For details of a detection
apparatus generally similar to the one depicted in FIG. 1, cross
reference is made to copending application Serial No. 630,892 filed
Apr. 14, 1967 of Lyle E. Packard, which although serving a purpose
different from that of the present invention, provides an
arrangement of components as to the detection mechanism which is
suitable for use in accordance with the present invention. Thus,
the detection apparatus 12, as viewed in FIG. 1, includes a base
assembly 14 which houses a pair of photomultipliers PMT No. 1, PMT
No. 2 disposed on opposite sides of a counting station 16.
The photomultipliers PMT No. 1, PMT No. 2 are mounted so as to
permit controlled simultaneous movement toward and away from the
counting station 16. To accomplish this, in the exemplary
arrangement the photomultipliers PMT No. 1, PMT No. 2 are
respectively mounted on depending brackets 18,20, the latter having
coaxially aligned, non-rotatable, internal oppositely-threaded
bearing sleeves 22,24 rigidly mounted therein. A parallely disposed
actuating shaft 26 passes through the aligned bearing sleeves 22,24
and is supported at one end in a bearing assembly 28 mounted on the
base assembly 14, and near its opposite end in a similar bearing
assembly 30 also rigid with the base assembly 14.
The end of the shaft adjacent the photomultiplier PMT No. 1 is
provided with a left hand threaded portion 32 threadbly engaging
with the bearing assembly 28 and bracket 18 supporting
photomultiplier PMT No. 1. The other portion of the shaft 26 is
provided with a right hand threaded portion 34 which is threadably
engaged with the bearing sleeve 24 and bracket 20 supporting the
photomultiplier PMT No. 2. The two brackets 18,20 and hence the
photomultiplier tubes are maintained free for controlled movement
toward and away from one another along a common axis while being
prevented from rotating about the axis of the actuating shaft 26 by
provision of a longitudinally extending track 36 fixedly mounted on
the base assembly 14 and slidably engaged in complimentary shaped
grooves 38,40 formed in the brackets 18,20.
In order to enable rotation of the actuating shaft 26, and hence
movement of the photomultiplier tubes linearly toward and away from
one another, one end of the actuating shaft portion 32 projects
slightly beyond its bearing support 28 and is drivingly coupled to
a gear train 42,44 (here shown only in diagramatic form, it being
understood that the particular means employed for driving shaft 26
could take any of well known conventional forms and could, if
desired, be mounted within the base assembly 14). Moreover, a
yieldable mounting may be employed to limit the movement of the
tubes toward each other on the basis of reaction forces applied
thereto. The movement of the photomultiplier tubes are more fully
discussed below permits close optical coupling with the sample
containers.
As illustrated in FIG. 1 the gear 44 is coupled to the drive shaft
46 of a conventional reversible servo motor 48 which receives its
input from a servo motor drive 50, the latter being provided with a
control signal derived from a control module (not shown).
Pursuant to the invention, the housing 61 (shown in phantom) is
provided with a sealed sample source supply chamber 62 adjacent the
input side of the detector and a sealed sample receiving chamber 64
attached to the housing 61 adjacent the output side of the
detector. A plurality of sample containers indicated generally at
66 are carried by a reel 68 in the supply chamber 62 and are
transferred in seriatim order through the counting or detecting
station 16 and then to a receiving reel 70 in chamber 64.
In order to move the sample containers from reel 68 to reel 70, the
latter is provided with a crank handle 72. However, it will be
appreciated that a suitable motor and control may be utilized for
automatic operation without departing from the invention.
Flexible Container Arrangement
In accordance with the present invention, the sample container is
in the form of a flexible bag made up of a layered,
light-transmissive polyester film which is heat sealed, welded or
otherwise suitably joined to define a closed flask between the
layers. To this end, referring to FIG. 2 there is shown in
prespective a portion of a strip of flexible containers 66
particularly suitable for use with the foregoing described detector
apparatus 10. The individual flexible sample containers 66 are
produced from a pair of strips 78,80 of polyester film such as
polyethylene terephthalate (Mylar) or any other suitable film
materials that are available to those skilled in the art. The
centrally disposed flask-like portion 76 is formed by joining the
layers 78,80 around its periphery, and it is preferably circular in
the plane of the layers with a diameter closely approximating the
diameters of the photomultiplier tubes. This arrangement enables
the flask portions to conform with the round photomultiplier tubes
and provides the optimum counting geometry for the container
structure.
In order to enable filling of the flask portions 76 with test
samples, the joining of the layers in the present instance is
provided with a discontinuity 82 that permits the injection of the
test samples into the centally located closed chamber 83 of the
flasks. To further facilitate filling operations, the joint line
defining the flask portion periphery may be brought up along
vertical or angular paths to the top of the strips so as to form
necks 84. After the flasks have been filled with the test samples,
sealing to prevent escape of the sample may be readily accomplished
by a transverse joint 86 across the neck 84 defining lines. Of
course, the joint may be made continuous in the first instance and
broken open for filling followed by resealing. On the other hand,
self-healing material may be employed to enable filling by a hollow
needle or the like.
A filled container 66, FIG. 3 assumes a generally pillow-shaped
configuration, viewed edgewise, wherein the side walls become
convex when the flask is as completely filled with liquid as
possible. The width of the strips 78,80 are preferably larger than
the diameter of flask portion 76 formed therein so that top and
bottom borders 88,90 respectively are left along the entire strip
of containers.
In accordance with one of the features of the present invention,
provision is made for the container strips to carry identifying
data or information which may be mechanically, magnetically,
optically or electrically handled for use. To this end, referring
to FIG. 4, there is illustrated one form of information carrying
arrangement for a flexible container strip 66 wherein the lower
border 90 is provided with a magnetic data recording strip 92. The
magnetic strip direcly beneath the flask portions is sufficient to
carry a recorded identification of the sample program instructions
and data for operating the counting apparatus and may also be used
to store any results obtained with the scintillation counting
apparatus.
Referring now to FIG. 5 there is illustrated another form of
information or identification carrying arrangement rendered
possible with the flexible container construction of the present
invention. Thus, it is here shown that the lower border 90 of the
flexible container strip 66 is provided with punched code
designations 94 which may be utilized with conventional and known
types of punched tape readers. Since the punched characters of the
arrangement of FIG. 5 as well as the magnetic strip arrangement of
FIG. 4 are integral with the flexible containers there is little
possibility that the data will be lost or misplaced.
Flexible Container Transference and Handling
In accordance with another feature of the present invention, the
provision of the flexible containers in a continuous strip enables
a simplified handling in the detection apparatus and eliminates the
need for much of the sample handling and transfer equipment which
was previously utilized with individual sample vials. As shown in
FIG. 1, the strip of flexible containers may be compactly stored on
a reel and the transference of the samples through the detector
apparatus involves a passing of the strip from reel to reel.
Moreover, the flexible containers may be produced individually or,
after production in a continuous strip, they may be severed into
individual units. When such individual units are produced, the
individual units may be attached to a conveyor strip or the like
that is adapted to pull them in sequential order through the
detector apparatus. An arrangement of that type is shown in FIG. 6
where a pair of wire-like elements 92,94 passing through sleeves
96,98, respectively, formed at the top and bottom borders of the
flexible containers 66 carrying individual containers in the same
manner as would a continuous strip.
As an alternative mechanical means for transferring a flexible
container 66 of the present invention through a detector apparatus,
there is shown in FIG. 7 an arrangement where a plurality of spaced
sprocket holes 100 are disposed along the lower border 90 of a
container strip which in the present instance is shown with a pair
of containers 76. The arrangement is such that conventional
strocket wheels or a belt with correspondingly spaced pins may be
utilized to pull the flexible containers through the counting
station 16 of a detection apparatus such as that viewed in FIG. 1.
In addition, as shown in FIG. 7 pilot holes 102 may be disposed at
the top border 88 of the strip to serve as locaters that insure
precise orienting of the flask portion 76 between the
photomultiplier tubes of the detection apparatus.
Detector Apparatus
Pursuant to the invention, close optical coupling with the flexible
containers 66 is achieved by "squeezing" contact between the
photomultiplier tubes and the flask portions 76 of the containers.
According to the invention, this may be accomplished by axially
shifting the photomultipliers with respect to a container in the
counting station. To this end, referring to FIG. 8, and FIG. 1
conjointly, it is seen that the flexible containers carrying the
test samples to be counted are brought into the counting station 16
of detector apparatus 10 in seriatim order. During the time when
the containers are being shifted into and out from the counting
station, the photomultiplier tubes PMT No. 1 and PMT No. 2 are in
the retracted, axially spaced apart position. When the flexible
container 66 holding the test sample to be analyzed is in the
counting station 16, the photomultiplier tubes are then moved
axially together until they are brought into intimate contact with
the outer faces of the flask portion 76, as shown in FIG. 9.
The photomultiplier tubes are brought together with sufficient
pressure so as to "squeeze" the flexible container 76 causing the
outer walls of the flask portion to conform with the faces of the
photomultiplier tubes. This results in an extremely close optical
coupling between the tubes and the container which minimizes lost
light at the contact surfaces of the container and tubes and high
quantitative accuracy of liquid scintillation counting results.
Of course, it will be appreciated that a similar result may be
achieved by maintaining the photomultiplier tubes in a fixed spaced
apart axial position and pulling the container into position in the
counting station between the faces of the tubes. If the spaced
apart distance between the tubes is preselected so as to be
slightly less than the thickness of the filled containers, the
squeezing will result from the pulling of the container between the
tube faces.
* * * * *