U.S. patent number 3,897,214 [Application Number 05/306,127] was granted by the patent office on 1975-07-29 for diagnostic device.
This patent grant is currently assigned to Boehringer Mannheim GmbH. Invention is credited to Hans Lange, Hans-Georg Rey, Walter Rittersdorf.
United States Patent |
3,897,214 |
Lange , et al. |
July 29, 1975 |
Diagnostic device
Abstract
Diagnostic agent for the detection of components contained in
liquids, e.g. body fluids, comprising a felt or fleece of
preponderantly polyamide fibers and impregnated with appropriate
reagents.
Inventors: |
Lange; Hans (Lampertheim,
DT), Rittersdorf; Walter (Mannheim-Waldhof,
DT), Rey; Hans-Georg (Mannheim-Waldhof,
DT) |
Assignee: |
Boehringer Mannheim GmbH
(Mannheim, DT)
|
Family
ID: |
5825931 |
Appl.
No.: |
05/306,127 |
Filed: |
November 13, 1972 |
Foreign Application Priority Data
|
|
|
|
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Nov 24, 1971 [DT] |
|
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2158124 |
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Current U.S.
Class: |
422/424; 28/166;
435/805; 436/97; 436/128 |
Current CPC
Class: |
G01N
33/521 (20130101); Y10T 436/200833 (20150115); Y10S
435/805 (20130101); Y10T 436/146666 (20150115) |
Current International
Class: |
G01N
33/52 (20060101); G01n 029/02 (); G01n 031/22 ();
G01n 033/16 () |
Field of
Search: |
;23/253TP,23B
;195/13.5R,127 ;117/14R,138.8R ;28/72NW |
References Cited
[Referenced By]
U.S. Patent Documents
Other References
Edelman G. M., Rutishauser U., and Millette C. F.; Cell
Fractionation and Arrangement on Fibers, Beads and Surfaces; Proc.
Nat. Acad. Sci. U.S.A. Vol. 68, No. 9, pp. 2153-2157, September
1971..
|
Primary Examiner: Scovronek; Joseph
Assistant Examiner: Lovercheck; Dale
Attorney, Agent or Firm: Burgess, Dinklage & Sprung
Claims
We claim:
1. Diagnostic device for the detection of components contained in
liquids, which diagnostic device comprises an absorbent carrier
comprising felt or fleece preponderantly consisting of polyamide
fibers, wherein said carrier is impregnated with appropriate
reagents.
2. Diagnostic device as claimed in claim 1 wherein said absorbent
carrier contains up to 50 percent by weight of fibers other than
polyamide fibers.
3. Diagnostic device as claimed in claim 2 wherein said other fiber
is at least one member of the group consisting of polyester fibers,
polyvinylchloride fibers and cellulose fibers.
4. Diagnostic device as claimed in claim 1 in which the felt or
fleece fibers are oriented directionally.
5. Diagnostic device as claimed in claim 1 in which the felt or
fleece fibers are randomly disposed.
6. Diagnostic device as claimed in claim 1 wherein the fibers of
the felt or fleece are bonded thermally or by means of bonding
agents.
7. Diagnostic device as claimed in claim 1 wherein said felt or
fleece is a needled felt or fleece.
8. Diagnostic device as claimed in claim 1 wherein said carrier is
impregnated with a wetting agent.
9. Diagnostic device as claimed in claim 1 wherein the reagents are
strongly alkaline phosphate buffer, sodium nitroferricyanide and
glycocoll, for carrying out Legal's test for ketonic bodies.
10. Diagnostic device as claimed in claim 1 wherein the reagents
are potassium bisulfate and p-dimethylaminobenzaldehyde, for
carrying out Ehrlich's test for urobilinogen.
11. Diagnostic device as claimed in claim 1 wherein said absorbent
composition is in the form of a narrow strip adhered to a synthetic
resin backing extending therefrom.
12. Diagnostic device as claimed in claim 1 wherein said absorbent
carrier is sealed between two synthetic resin films.
13. Diagnostic device as claimed in claim 1 wherein said absorbent
carrier is sealed between a synthetic resin film and a synthetic
resin mesh.
14. Diagnostic device as claimed in claim 9 wherein said absorbent
carrier consists essentially of pure polyamide felt or fleece.
15. Diagnostic device as claimed in claim 10 wherein said absorbent
carrier is a mixed felt or fleece also containing polyester
fibers.
16. Diagnostic device as claimed in claim 1 wherein the polyamide
is of the nylon type.
17. Diagnostic device as claimed in claim 1 wherein the polyamide
is of the perlon type.
Description
The present invention is concerned with diagnostic agents for the
detection of components in liquids, especially in body fluids,
comprising an absorbent carrier impregnated with reagents.
Test strips impregnated with suitable reagents have been in use for
a long time for detecting components of liquids. pH indicator
papers are used very widely but other reagent papers are also
employed. In recent years, test papers have achieved great
importance for the detection of glucose, protein, nitrite and the
like in body fluids, for example in urine and blood, because they
enable the physician to carry out a rapid and simple diagnosis of
metabolic disturbances outside the laboratory.
For the various tests, a large number of absorbent carriers has
been proposed, for example, wood, asbestos, gypsum, glass fiber
felts, synthetic resin fleeces and the like, but in actual practice
filter paper is used almost exclusively for the commercially
available test strips. The reason for this is that, in addition to
the cheapness and ease of working up of paper, the reagents on the
cellulose fibers of the paper are especially reactive.
Although filter paper is usually the best carrier material, there
are chemical test reactions which cannot be carried out on paper.
Quite apart from test reagents which, in a state of fine division,
are unstable in air and, therefore, cannot be applied to a carrier
by impregnation, there are a number of test reagents which destroy
paper fibers and make the paper brittle. Such reagents include, for
example, strongly alkaline, strongly acidic and oxidizing
substances and especially reagent mixtures with a high salt
content.
Tests which are especially important for medical diagnosis but
which cannot be carried out or cannot be carried out satisfactorily
with the use of test strips include, for example, Legal's test for
ketonic bodies and Ehrlich's test of pyrrole bodies. Legal's test
requires, for a satisfactory functioning, a high concentration of a
strongly alkaline buffer in the reaction solution and Ehrlich's
test requires a high concentration of a weakly acidic compound, for
example, oxalic acid or potassium bisulfate.
Filter papers which have been impregnated with the high salt
concentrations necessary for these test admittedly show a rapid and
sensitive reaction with the substrates in question but are so hard
and brittle that they cannot be bent, folded or cut up without
breaking and crumbling. Since not only in the production but also
in the transport and use of such strips, mechanical stresses
constantly occur, the usefulness of such strips is severely
limited.
Attempts to make such papers mechanically more stable by laminating
on to a synthetic resin film were unsuccessful because the hard
paper, upon bending or folding, again separated or crumbled away
from the film. Cellulose fleece strengthened with synthetic resin
fibers, for example, with polyvinyl chloride or polyester fibers,
also have not proved to be advantageous because they still did not
possess a sufficient degree of stability.
The obvious complete replacement of cellulose by more stabel
synthetic resin fibers, for example by polypropylene fibers, such
as has been propsed in German Patent Specification No. 2,007,013
for a bilirubin test, also do not prove to be successful in the
case of the above mentioned tests since these test strips show a
sufficient mechanical stability but only showed very weak color
changes which, in the lower concentration ranges, could no longer
be evaluated as an indication of bilirubin.
Microscopic investigations have shown that the important difference
between the cellulose fibers of paper and synthetic resin fibers is
that the cellulose fibers swell during the impregnation and a part
of the reagents is embedded or incorporated into the fibers,
whereas in the case of synthetic resin fibers, the reagents are
only deposited on the surfaces of the fibers.
After laborious and fruitless experiments with a very large variety
of absorbent materials, especially with various synthetic resin
fleeces, we have now, surprisingly, found, that the felts or
fleeces of the instant invention differ significantly from all of
the other materials tested.
The present invention provides diagnostic agents comprising an
absorbent carrier which consists entirely or preponderantly of
polyamides. Only these fleeces or felts based on polyamides provide
the final test strips with a sufficient degree of stability,
without destroying the reactivity and sensitivity of the test
reagents.
It is not known why polyamides differ in this manner from other
synthetic resins, for example from polyesters and polyvinyl
chlorides. The difference is extremely surprising because the
reagents are also not embedded or incorporated into the polyamide
fibers.
As polyamides to be used according to the present invention, there
can be employed not only those of the nylon type, made from
dicarboxylic acids and diamines, but also those of the perlon type,
made from .omega.-aminocarboxylic acids. As mixture components in
mixed felts and fleeces, it is especially preferred to use
polyester fibers but fibers of other synthetic resins, for example
of polyvinyl chloride and the like, can also be admixed in amounts
of up to 50%.
The felts and fleeces can be produced not only be wet-depositing
but also by dry-depositing, the fibers can be oriented or lie at
random and they can be connected thermally or by binding agents or
they can be needled.
The felt or fleece selected depends essentially upon the nature of
the reagents to be used for the impregnation thereof. From the
large number of commercially available felts and fleeces the best
one can easily be determined by a few simple preliminary
experiments.
Thus, in the case of impregnation with acidic salts, for example
with potassium bisulfate in the case of the urobilinogen test, it
is recommended to use a mixed felt or fleece containing polyester
fibers. On the other hand, in the case of basic salts, such as are
used for the ketonic body test, pure polyamide felt or fleece has
proved to be the best. In special cases, a mixed felt or fleece of
polyamide fibers and cellulose fibers can also be used.
The thickness and weight per unit area of the felt or fleece used
can be varied. However, very thin or light felts and fleeces take
up very little reagent and thus have a poorer reactivity. Thick or
voluminous felts and fleeces, on the other hand, take up large
amounts of reagents and it is thus more difficult to work them up.
Here again, the most suitable felt or fleece for any particular
case can be easily determined by a few simple preliminary
experiments.
The felt or fleece can be impregnated in conventional manner.
However, in order to improve wettability, it is sometimes desirable
either to add a wetting agent to the impregnation solution or first
to impregnate the felt or fleece with a wetting agent.
The impregnated felt or fleece is then dried in the usual manner.
If desired, it can be cut up into narrow strips and used directly
or, as still smaller pieces, can either be stuck on to a handle of
synthetic resin or, according to German Patent Specification No.
1,546,307, can be sealed between synthetic resin films or,
according to German Patent Specification No. P 21 18 455.4, can be
sealed between a synthetic resin film and a synthetic resin
mesh.
The following Examples are given for the purpose of illustrating
the present invention, the properties of the fleeces and felts used
in the following Examples being summarized in Table 4 given
hereinafter.
EXAMPLE 1
Urobilinogen Test
The materials set out in the following Table 1 were impregnated
with an aqueous solution containing, per 100 ml, 20 g potassium
bisulfate and 0.2 g p-dimethylaminobenzaldehyde. The properties of
the impregnated test strips, as well as their reactions with
urobilinogen-containing urine, were also set out in the following
Table 1.
TABLE 1 ______________________________________ material and
stability reaction material number
______________________________________ filter paper (1)* very
brittle good becomes grey uniform polyester fleece (9) stable weak
non-uniform polyester-polyamide very good fleece (8) stable uniform
______________________________________ *The material numbers are
described in Table 4, infra.
EXAMPLE 2.
Ketonic Body Test
The materials set out in the following Table 2 were impregnated
with Solution I, dried, impregnated with Solution II and again
dried.
______________________________________ Solution I trisodium
phosphate dodecahydrate 21.0 g disodium hydrogen phosphate
dihydrate 11.2 g glycocoll 18.7 g distilled water ad 100.0 ml
Solution II sodium nitroferricyanide dihydrate 0.9 g
polyvinylpyrrolidone-vinyl acetate copolymer (50% solution in
ethanol) 6.5 ml organic phosphate ester of anionic surface-active
agent (10% solution 1.7 ml in ethanol) dimethyl sulfoxide 38.0 ml
ethanol 18.5 ml distilled water ad 100.0 ml
______________________________________
The properties of the impregnated test strips and the reactions
with urine containing increasing amounts of acetoacetate or of
acetone were also set out in the following Table 2.
TABLE 2 ______________________________________ material and
stability reaction material number
______________________________________ filter paper (1) very
brittle very good uniform polyester fleece (9) stable weak
non-uniform polyamide fleece (5) stable good uniform
______________________________________
EXAMPLE 3
Ketone Body Test
The materials set out in the following Table 3 were impregnated
with Solution I, dried, again impregnated with Solution II and
again dried.
______________________________________ Solution I tetrasodium
ethylenediamine- tetraacetate 38.5 g glycocoll 18.7 g distilled
water ad 100.0 ml Solution II sodium nitroferricyanide dihydrate
1.0 g dimethyl formamide 40.0 ml methanol ad 100.0 ml
______________________________________
The following Table 3 also shows the mechanical properties and the
reactions of the test strips with urine which contains increasing
amounts of acetoacetate or of acetone.
TABLE 3 ______________________________________ material and
properties reaction material number
______________________________________ filter paper (1) very
brittle very good cellulose-cottonwool very brittle still good
fleece (2) regenerated cellulose- polyvinyl chloride rather brittle
good fleece (3) regenerated cellulose- polyamide fleece (4)
scarcely brittle very good polyamide fleece (5) stable very good
polyamide fleece (6) stable good polyamide fleece (7) stable still
good polyester-polyamide fleece (8) stable good polyester fleece
(9) stable weak polyester fleece (10) stable weak
______________________________________
The following Table 4 gives the properties and description of the
various carrier materials used in the above Examples:
TABLE 4
__________________________________________________________________________
material material type producer synthetic working up thickness wt.
per No. (in Western resin mon- in mm. unit Germany) omers and area
in average g/m.sup.2 mol. wt.
__________________________________________________________________________
1 filter paper 23SL Schleicher -- random, with 0.45 230 &
Schull wet tear strength agent 2 cellulose- Paratex Lohmann --
longitudinally 0.2 190 cottonwool III/50 KG laid with fleece (7:3)
binding agent 3 regenerated VS 446 Binzer vinyl random, with 0.5
100 cellulose- chloride binding agent polyvinyl M.W. about chloride
100,000 fleece (95:5) 4 regenerated (sample) Binzer
.epsilon.-capro- random, with 1.0 150 cellulose- lactam binding
agent polyamide M.W. about fleece (1:1) 22,000 5 polyamide V 27835
C. Freu- .epsilon.-capro- longitudinally 0.35 100 fleece (sample)
denberg lactam and transverse- M.W. about ly laid, therm- 20,000
ally strength- ened 6 polyamide N 933c C. Freu- .epsilon.-capro-
longitudinally 0.5 95 fleece (sample) denberg lactam and
transverse- M.W. about ly laid, therm- 20,000 ally strength- ened 7
polyamide FT 2114 C. Freu- .epsilon.-capro- longitudinally 0.25 80
fleece denberg lactam and transversely M.W. about laid, thermally
20,000 strengthened 8 polyester- Suprotex Kalle terephthalic
longitudinally 1.5 300 polyamide acid-ethylene and transversely
fleece glycol M.W. laid, thermally (1:1) about 18,000 strengthened,
adipic acid- needled, without hexamethylene- binding agent diamine,
M.W. about 20,000 9 polyester E 5209 Kalle terephthalic needled,
therm- 0.35 250 fleece (sample) acid-ethylene ally strengthened
glycol M.W. about 18,000 10 polyester H 1015 C. Freu- terephthalic
longitudinally 0.25 170 fleece denberg acid-ethylene and
transversely glycol M.W. laid, thermally about 18,000 strengthened
__________________________________________________________________________
It will be understood that the specification and examples are
illustrative but not limitative of the present invention and that
other embodiments within the spirit and scope of the invention will
suggest themselves to those skilled in the art.
* * * * *