U.S. patent number 3,891,140 [Application Number 05/446,369] was granted by the patent office on 1975-06-24 for centrifuge.
This patent grant is currently assigned to Becton, Dickinson and Company. Invention is credited to Waldemar A. Ayres.
United States Patent |
3,891,140 |
Ayres |
June 24, 1975 |
Centrifuge
Abstract
A centrifuge for serum/plasma separator tubes in which racks
holding one or more tubes can be inserted into slots in the
rotatable member of the centrifuge. The slots of the rotatable
member are positioned to provide dynamic balancing when all the
slots are filled with racks of tubes. When the slots are not all
filled with racks of tubes, the centrifuge is so designed that such
racks can be positioned opposite each other to maintain such
balance. In a modified form, the entire rotatable member loaded
with racks of tubes can be loaded onto or removed from the
centrifuge as a unit, thereby providing a quick, convenient and
simple way of handling a full load of tubes at one time.
Inventors: |
Ayres; Waldemar A. (Rutherford,
NJ) |
Assignee: |
Becton, Dickinson and Company
(East Rutherford, NJ)
|
Family
ID: |
23772331 |
Appl.
No.: |
05/446,369 |
Filed: |
February 27, 1974 |
Current U.S.
Class: |
494/12;
494/16 |
Current CPC
Class: |
B04B
5/0414 (20130101) |
Current International
Class: |
B04B
5/04 (20060101); B04B 5/00 (20060101); B04B
005/00 () |
Field of
Search: |
;233/1R,26,27,28 |
References Cited
[Referenced By]
U.S. Patent Documents
Primary Examiner: Krizmanich; George H.
Attorney, Agent or Firm: Kane, Dalsimer, Kane, Sullivan and
Kurucz
Claims
Having thus described the invention, what is claimed is:
1. A centrifuge for liquid separator tubes comprising:
drive means,
a rotatable member mounted on the drive means,
said rotatable member having an even numbered plurality of
uniformly spaced supports around the periphery of the rotatable
member with opposite supports equidistant from the axis of the
drive means,
a rack adapted to hold a plurality of separator tubes spaced from
each other,
said rack having means to mount the rack in the said supports of
the rotatable member in an attitude normal to the said axis,
and
means to secure the racks in the supports,
said rack comprising:
a. a pair of vertical side members spaced from each other by a
distance greater than the diameter of said separator tubes and
having a height less than the height of said separator tubes;
b. a base member connecting the said side members and adapted to
support the separator tubes during loading, unloading and
centrifugation;
c. a longitudinal guide member spaced from the base member and
supported by the side members and adapted to evenly space the
bottoms of the separator tubes; and
d. a top rack member having a series of holes larger than the
separator tubes and adapted to space said tubes;
e. said top rack member being secured to bent over edge portions of
said vertical side members to form a pair of flanges adapted to fit
into cooperating slots in the said supports of the rotatable member
and retain the rack in the rotatable member.
2. The centrifuge of claim 1 in which the rotatable member is
detachably mounted to the drive means whereby the said member
loaded with racks of tubes can be removed from or loaded onto the
centrifuge as a unit.
3. The centrifuge of claim 2 which includes handle means detachably
secured to the said rotatable member whereby the rotatable member
and racks may be lifted in and out of the centrifuge as an
assembly.
4. The centrifuge of claim 3 wherein the handle means comprise two
handles located along a diameter passing through the axis of said
rotatable means and each handle is located equidistant from said
axis.
5. The centrifuge of claim 4 wherein the handle means includes a
pair of T-shaped portions adapted for comfortable and efficient
lifting by the two hands of a user.
6. The centrifuge of claim 5 wherein the T-shaped portion of each
handle means is insertable into the rotatable member and includes a
boss adapted to limit the distance the handle means can be inserted
into the rotatable member.
7. The centrifuge of claim 1 in which each support comprises a
cutout in the rotatable member parallel to the axis of the drive
means to receive the said rack, and which includes safety closure
means detachably mounted to close the upper ends of the said
cutouts so that the racks are secured in position while the
rotatable member is spinning.
Description
BACKGROUND OF THE INVENTION
The prior art includes the following pertinent U.S. Pat. Nos.
3,674,198 3,151,073 3,643,812 3,142,385 3,474,913 3,050,239
3,454,217 2,755,018 3,339,837 2,560,988 3,289,927 486,390
None of these patents show or describe a centrifuge head embodying
the means, hereinafter described and claimed, for mounting a
plurality of racks holding a plurality of separator tubes on the
periphery of the head and for holding such racks on the head during
rotation of the head. Furthermore, such patents do not disclose the
specific design of the racks for holding the plurality of
tubes.
SUMMARY OF THE INVENTION
It is an object of the present invention to provide a centrifuge
for separator tubes designed to hold a plurality of racks or tubes
on the periphery of a rotatable member, which racks can be
positioned on the said member to maintain dynamic balance during
rotation of the centrifuge.
It is a further object to provide racks for the centrifuge which
will hold a plurality of tubes and are so designed that they can be
fitted into support means on the periphery of the rotatable member
of the centrifuge.
It is a further object to provide means on the centrifuge for
holding the racks and tubes so that they cannot leave the support
means during centrifugation.
It is a further object to provide a centrifuge with a removable
rotatable member which will hold racks of tubes so that all the
racks and tubes supported on the rotatable member can be removed as
a unit to facilitate loading and unloading of the centrifuge.
BRIEF DESCRIPTION OF THE DRAWINGS
Other objects and advantages will become apparent from the
following description which is to be taken in conjunction with the
accompanying drawings, in which:
FIG. 1 is a perspective view, partly broken away, of the centrifuge
of the present invention;
FIG. 2 is a side elevation, partly in section, showing the
centrifuge with one rack of tubes in place on one side and the rack
on the opposite side removed;
FIG. 3 is a top plan view of a portion of the centrifuge showing
how the racks of tubes are inserted downwardly into the top of the
centrifuge;
FIG. 4 is a perspective view of the rack of tubes which is inserted
into the centrifuge;
FIG. 5 is a side elevation, partly in section, of the tube rack
containing six tubes;
FIG. 6 is a view, partly in section, along the line 6--6 of FIG.
5;
FIG. 7 is a top plan view of the tube rack;
FIG. 8 is a perspective view, partly broken away, of a carrier for
holding a plurality of tube racks; and
FIG. 9 is a perspective view of a modified form of centrifuge in
which the entire rotatable member holding the tube racks can be
removed as a unit.
DESCRIPTION OF THE PREFERRED EMBODIMENT
Referring to FIGS. 4-7, serum/plasma separator tubes 10, provided
with top and bottom stoppers 10a and 10b respectively, are shown
resting in a rack 11. This rack may be formed of a strong
non-corrodable material, for example stainless steel, It consists
of a bottom 12, side members 13--13 and flanges 14--14. The
opposite side members 13 are spaced to provide adequate clearance
for the tubes. The rack, as shown, is of a size to hold six tubes,
but it will be understood that such size may be varied to hold more
or fewer tubes. A flat plate 15, preferably also made of stainless
steel, extends across the top surfaces of the flanges 14 and is
rigidly secured to them by any suitable means, for example by
welding. The top plate 15 is provided with a plurality of holes 16
to receive the tubes 10 and keep them uniformly spaced from each
other. These holes are of a size that they will provide sufficient
clearance to receive the lower stoppers of the tubes 10. Secured to
the bottom 12 of the rack is a spacer guide 17 having a plurality
of holes 18 corresponding in number to the holes 16 in the top
plate 15. The holes 16 and 18 are aligned so that the tubes
inserted into the top plate 15 and the spacer guide 17 will keep
the tubes parallel and uniformly spaced.
Referring to FIG. 2, there is a motor housing 19 from which extends
the motor shaft 20. The motor enclosed within the housing is of a
conventional type for driving a centrifuge and is not illustrated
or described. A rotatable member 21 fits onto the motor shaft 20,
being vertically supported by the snap ring 22 or some other
suitable means. The rotatable member 21 has an even numbered
plurality of uniformly spaced cutouts 21a around the periphery
(FIG. 3). On each side of each cutout is a guide slot 23 (FIGS. 2
and 3) to receive each tube rack. The lower ends of the guide slot
23 are closed by the circular ring member 24 which is secured to
the rotatable member 21 by screws 25 or other suitable means. The
circular ring member 24 also acts as a stiffening and restraining
member resisting the outward thrust of centrifugal force on the
lower portions of the rotatable member 21.
Further referring to FIG. 2, there is shown a rack 11 of six tubes
10, rotated 90.degree. from the vertical position shown in FIG. 4
and inserted into the rotatable member 21. The flanges 14 (FIG. 4)
and the ends of the top plate 15 fit into the mating slots 23 (FIG.
2) of the rotatable member. The rack is lowered into the rotatable
member until it comes to rest against the circular ring member
24.
The vertical height of the spacer guide 17 is made to cooperate
with the central rotatable member 21 and the motor housing 19 so
that, during loading of a tube rack, if an individual tube is
jostled radially inward until the top stopper contacts the motor
housing 19, part of the tube's lower stopper is still within its
hole in the guide 17. Then when the centrifuge motor starts to
rotate, the displaced tube will be thrust radially outward by
centrifugal force and will automatically reposition itself in the
proper location.
After the tube racks 11 (FIG. 1) are positioned in the slots of the
rotatable member a cover 26 is placed on top of the rotatable
member closing the upper ends of guide slots 23. Both the cover
member 26 and the rotatable member 21 are secured to the motor
shaft 20 by screwing the knurled and slotted knob 27 into the top
of the shaft (FIG. 2).
The motor housing 19 (FIG. 2) rests on a base plate 28 from which
projects upwardly a shell 29 which serves as a protective shroud
around the centrifuge.
When all eight tube racks with their 48 tubes are loaded into the
centrifuge as heretofore described and the cover 26 is secured with
the knob 27, the centrifuge is spun at the speeds and time
intervals desired (which may be automatically programmed) so that
the different liquids and/or solids are separated in all the tubes.
Then the centrifuge is stopped and the racks of tubes are unloaded
and placed in a tube rack carrier 30 (FIG. 8) for transfer to the
location where tests are to be performed or the different elements
are to be analyzed or handled.
The apparatus has great flexibility for centrifuging fewer tubes
than a full load of 48 tubes. Any pairs of racks may be omitted as
long as the empty regions are ones symmetrically across a diameter
of the centrifuge, so that dynamic balancing will be preserved.
Also two opposite racks having less than six tubes can be used as
long as the same number of tubes are in each and they are in the
same respective locations in each rack to maintain dynamic
balancing. If the number of blood-filled tubes on hand to be
centrifuged is an uneven number, an additional tube can be filled
with water to provide a matching weight and this tube can be used
with the others to provide an even number of tubes in total, for
handling as described above. Thus, the apparatus and method of the
present invention has complete flexibility in being able to
centrifuge any number of tubes at one time, from one up to a
maximum of 48 tubes.
Referring to FIG. 8, the tube rack carrier 30 has a base 31, end
members 32 and a top 33. The top 33 has a plurality of cutouts 34
spaced from each other and each sized to receive a tube rack.
Secured to the base 31 are a pair of spaced, parallel guide members
35 which run the length of the base. Extending vertically from
these members 35 are tabs 36 with beveled corners. These tabs are
located to guide the tube racks 11 into the correct positions as
they are inserted into cutouts 34 and lowered to the bottom of the
carrier. When a tube rack is being lowered through the cutout 34 it
can only be rocked right or left. If it comes to rest on top of a
tab 36, the top of the tube rack will be so much higher than normal
and higher than other tube racks that it will be immediately
noticeable and the operator will then shift it to its proper
position.
The above description has covered the loading and unloading of the
centrifuge by the insertion or removal of the tube racks
individually. In such case the rotatable member 21 has remained
affixed to the centrifuge. As an alternative, the entire rotatable
member 21 loaded with tube racks and tubes can be removed from the
centrifuge and replaced with another pre-load rotatable member, so
that the second load can be centrifuged while the first load is
being removed from the first rotatable member and replaced with a
third set of serum separators. The removal of the entire rotatable
member and its load can be accomplished by providing for the
insertion of a pair of handles into the member by which the member
can be lifted from the centrifuge, as shown in FIG. 9. The top of
the rotatable member is provided with a pair of drilled tapped
holes 37 (FIGS. 2 and 3), one on each side of the member. A
T-shaped handle 38 (FIG. 9), having a threaded portion and a collar
or boss 38a, which limits how far the handle can be screwed into
the hole 37 (FIG. 2), is used to lift the member 21 (FIGS. 2 and 9)
on each side. The knob 27 (FIG. 2) and the cover 26 are first
removed, and handles 38 (FIG. 9) are screwed into the holes 37
(FIG. 2) and the entire rotatable member 21 (FIG. 9) with its load
is removed from the centrifuge. It is not necessary for the handles
to be screwed so that the bosses 38a are tight against the
rotatable member. It is only necessary for the handles to be
screwed in sufficiently to prevent the handles from becoming
detached from the member. The handles can be oriented so that the
cross members are approximately parallel. One handle can then be
grasped in each hand with two fingers and the entire rotatable
member lifted from the centrifuge and placed on an adjacent work
table for loading and reloading. After is has been reloaded it is
reinstalled on the motor shaft by reversing the steps described
above.
The centrifuge of the present invention has been described and
illustrated in connection with serum/plasma separator tubes used in
the separation of blood into its light and heavy phases. However,
it will be understood that the centrifuge can also be used for the
separation of other liquids into their various phases.
It will be obvious to those skilled in the art that various
modifications may be made to the apparatus hereinbefore described.
Such embodiments are considered to be within the scope of the
invention. The above invention is not to be limited except as set
forth in the following claims.
* * * * *