U.S. patent number 3,890,954 [Application Number 05/358,350] was granted by the patent office on 1975-06-24 for method of and apparatus for collecting cultures.
This patent grant is currently assigned to U.S. Medical Research & Development, Inc.. Invention is credited to Donald J. Greenspan.
United States Patent |
3,890,954 |
Greenspan |
June 24, 1975 |
Method of and apparatus for collecting cultures
Abstract
A swab is packaged in a tube having an open end and containing a
culture-sustaining liquid at the bottom of the tube and a plug
including a one-way isolating valve located above the liquid. After
removal of the swab from the tube and swabbing of a body canal or
the like with the absorbent tip of the swab, the swab may be placed
back in the tube with the absorbent tip adjacent the plug. The plug
may then be forced downwardly through the liquid by pressing on the
end of the swab or another liquid member telescoped within the tube
so as to force the liquid up through the valve into contact with
the absorbent tip. A closure or cap which may be used to press on
the end of the stick or the rigid member then forms a seal at the
open end of the tube.
Inventors: |
Greenspan; Donald J.
(Riverside, NJ) |
Assignee: |
U.S. Medical Research &
Development, Inc. (Riverside, NJ)
|
Family
ID: |
23409312 |
Appl.
No.: |
05/358,350 |
Filed: |
May 8, 1973 |
Current U.S.
Class: |
600/572;
604/1 |
Current CPC
Class: |
A61B
10/02 (20130101); A61B 10/0096 (20130101); C12M
23/32 (20130101); C12M 45/22 (20130101); C12M
33/02 (20130101); B01L 3/5029 (20130101); B01L
2400/0694 (20130101); G01N 2001/028 (20130101); A61B
2050/314 (20160201); B01L 2200/185 (20130101); B01L
2400/0638 (20130101); B01L 2400/0478 (20130101); A61F
13/38 (20130101) |
Current International
Class: |
C12M
1/26 (20060101); A61B 10/00 (20060101); C12M
1/30 (20060101); A61B 19/02 (20060101); A61F
13/38 (20060101); A61B 19/00 (20060101); G01N
1/02 (20060101); A61b 010/00 () |
Field of
Search: |
;128/2W,2F,269
;195/13.5R,139 |
References Cited
[Referenced By]
U.S. Patent Documents
Foreign Patent Documents
Primary Examiner: Howell; Kyle L.
Attorney, Agent or Firm: Woodcock, Washburn, Kurtz &
Mackiewicz
Claims
What is claimed is:
1. An apparatus for collecting cultures including an elongated
member having an absorbent swabbing tip received within a container
tube having an open end, a closed end and sides therebetween, a
culture-sustaining liquid at the closed end of the tube, the
improvement comprising plug means within said tube adjacent said
culture-sustaining liquid, a portion of said plug means extending
outwardly toward the sides of said tube in resilient contact
therewith so as to form a sliding, substantially liquid-tight
junction with the sides of said tube, said plug means including a
resilient isolating valve member closing an opening in said plug
means, said resilient isolating valve member being closed when said
plug means is stationary within said tube so as to create
substantial physical isolation of the swab within the tube from the
culture-sustaining liquid when the plug means is located above the
culture-sustaining liquid, said resilient isolating valve member
opening in response to movement of said plug means toward the
bottom of said tube through said culture-sustaining liquid so as to
moisten said absorbent swabbing tip of said swab with said
culture-sustaining liquid, said plug means having a surface
adjacent said tip tapering radially inwardly toward said closed end
for guiding the absorbent tip toward the center of said plug means
to enable said elongated member to transmit a substantially axial
force for moving said plug means through the culture-sustaining
liquid.
2. The apparatus of claim 1 further comprising a closure member
having a surface tapering radially inwardly away from the end of
said elongated member opposite said absorbent swabbing tip for
guiding said end of said elongated member toward the center of said
closure member thereby enabling the elongated member to transmit a
substantially axial force from said closure member to said plug
means when said closure means is pushed on said tube.
3. The apparatus of claim 1 wherein said isolating valve member is
spaced from the center of said plug means such that pressure
applied at the center of said plug means by said swab tends to open
said valve member.
4. The apparatus of claim 1 including more than one said valve
member closing more than one said opening in said plug means, each
valve member being spaced from another and the center of said plug
means to assure that at least one valve member will open.
5. The apparatus of claim 4 wherein said plug means includes holes
extending upwardly from the bottom of the plug means and
terminating in each said valve member, each said valve member
comprising a flap extending across one of said holes to said
tapered surface of said plug means and formed in part
therefrom.
6. The apparatus of claim 1 wherein said plug means includes at
least one hole extending upwardly from the bottom of the plug means
and terminating in at least one said valve member, said valve
member comprising a flap extending across said hole and formed in
part from the upper surface of said plug means.
7. An improved method of collecting cultures in a live condition
utilizing an apparatus comprising a collection tube having sides
and a botton containing a culture-sustaining liquid adjacent the
bottom of the tube, a plug located within the tube above the liquid
and in resilient contact with the sides of the tube, the plug
including an isolating valve remaining closed when the plug is
stationary above the liquid and opening as the plug is moved
downwardly through the liquid, and a swab including an elongated
member having an absorbent tip at one end thereof and adapted to be
disposed within the tube above the plug, and closure means for
sealingly engaging the open end of the tube, the improved method
comprising the steps of:
swabbing an area of culturable material with the absorbent swabbing
tip of the swab;
inserting the swab into the tube;
transmitting a downward force to the plug through the swab so as to
open the isolating valve and permit the liquid to saturate the
absorbent tip of the swab;
metering a selected amount of culture-sustaining liquid into
saturating contact with the absorbent tip as a function of the swab
position, and
closing said valve when said swab comes to rest in said tube so as
to substantially isolate any culture-sustaining liquid remaining
adjacent the bottom of the tube from said swab.
8. The method of claim 7 including the step of applying said
closure means and transmitting said downward force through said
closure means to said swab.
9. Apparatus for collecting cultures and the like comprising:
a hollow tubular container having a closed end, an open end and
sides extending therebetween;
a culture-sustaining liquid positioned within said tubular
container adjacent said closed end;
piston means forming a swab-receiving chamber within said hollow
tubular container, said chamber being substantially isolated from
the sides of said tubular container, said piston means including a
plug portion adjacent said culture-sustaining liquid and in
resilient contact with the sides of said tube so as to form a
sliding, substantially liquid-tight junction with the sides of said
tube, said plug portion having an opening therethrough for
communication between said swab-receiving chamber and said
culture-sustaining medium, said plug portion including a resilient
isolating valve member in said opening, said valve member remaining
closed when said piston means is stationary within said tubular
container; and
a swab having an absorbent swabbing tip with at least said tip
being enclosed within said chamber,
said resilient isolating valve member adapted to open as said
piston means is advanced through said culture-sustaining medium
toward said closed end so as to allow said culture-sustaining
medium to flow through said opening to moisten said absorbent tip
of said swab enclosed within said chamber.
10. The apparatus of claim 9 wherein said piston means comprises a
tubular portion contacting said plug portion and extending along a
substantial length of said swab.
11. The apparatus of claim 10 wherein said piston means further
comprises a cap closing said tubular portion and said tubular
container at said open end thereof.
Description
BACKGROUND OF THE INVENTION
This invention relates to a swabbing method and apparatus for use
by physicians and technicians for collecting a culture from various
areas of a patient's body, such as the ears, the nose and throat,
and for keeping the culture moist and alive for a period of time
after it is collected.
In general, this is accomplished through the use of a container
which receives the culture-carrying swab and bathes the swab in a
culture-sustaining liquid. U.S. Pat. No. 3,450,129 -- Avery et al.
discloses a particular swabbing unit for this purpose. The unit
includes a container which carries its own sealed supply of liquid
in a frangible ampoule along with a swab, all of which is packaged
in a sanitary wrapper. After the swab has been removed from the
wrapper and container and the culture has been taken, the swab is
inserted back into the container, a cap is applied to the end of
the container and the frangible ampoule is broken so as to bathe
the absorbent tip of the swab in the culture-sustaining liquid
which was incapsulated in the ampoule.
SUMMARY OF THE INVENTION
It is therefore one object of this invention to provide a swabbing
method and apparatus for taking cultures which is economical.
It is another object of this invention to provide such a method and
apparatus which may sustain the life of a culture for an extended
period of time.
In accordance with one important aspect of the invention, a
container tube is provided for receiving a culture-carrying swab
comprising a tube having an open end and closed bottom, a
culture-sustaining liquid at the bottom and a plug in sealing
engagement with the walls of the tube above the upper level of the
liquid. The plug includes a one-way isolating valve closing an
opening through the plug so as to permit the flow of the liquid
through the plug into contact with the absorbent tip of the swab as
the plug is forced downwardly toward the bottom of the tube.
In one embodiment of the invention, an inner tube is telescoped
within the container tube with the swab located centrally in the
inner tube. The inner tube may then be used to push the plug
through the liquid by pushing on the upper end with the closure
member.
In another embodiment of the invention, the swab itself is utilized
to push the plug down through the liquid. Once again the closure
member may be utilized to push on the upper end of the swab. In
order to assure that the force on the plug is substantially axially
directed at the center of the plug to avoid skewing of the plug
within the container tube, the interior of the cap is tapered
upwardly to a centrally located surface. Similarly, the upper
surface of the plug may be tapered downwardly toward a centrally
located surface.
In accordance with another important aspect of this invention, the
closure member and the container tube are provided with elongated
sealing surfaces so as to allow the closure member to be moved
downwardly toward the bottom while maintaining a seal. The closure
in turn forces the inner tube or the swab downwardly so as to move
the plug closer to the botton. In this manner, fresh
culture-sustaining liquid may be periodically introduced into
contact with the absorbent tip of the swab so as to assure that the
tip is maintained in a moist condition. For this purpose, marks may
be applied to the upper end of the container tube to indicate the
various positions to which the closure member may be periodically
moved.
BRIEF DESCRIPTION OF THE DRAWINGS
FIG. 1 is a perspective view of a swabbing apparatus embodying the
invention in its wrapper;
FIG. 2 is a sectional view of the swabbing apparatus embodying the
invention;
FIG. 2a is an enlarged view of a valve in the plug of the swabbing
apparatus shown in FIG. 2;
FIG. 3 is a sectional view of the swabbing apparatus of FIG. 2
after the culture-carrying swab has been sealed into its container
tube;
FIG. 4 is a sectional view of another closure member for the
container tube of FIGS. 2 and 3;
FIG. 5 is a partial elevational view of the markings on the
container tube of FIGS. 2 and 3;
FIG. 6 is a sectional view of another swabbing apparatus embodying
the invention before the swab has been bathed in a
culture-sustaining liquid;
FIG. 7 is a sectional view of the swabbing apparatus of FIG. 6
after the swab has been bathed in a culture-sustaining liquid;
FIG. 8 is a sectional view of a modified plug which may be utilized
with the swabbing apparatus shown in FIGS. 6 and 7;
FIG. 9 is a sectional view of a swab which is permanently affixed
to a closure member which may be utilized with the container tube
of FIGS. 6 and 7;
FIG. 10 is a sectional view of another modified plug which may be
utilized with the swabbing apparatus shown in FIG. 9;
FIG. 11 is a bottom view of the plug of FIG. 10;
FIG. 12 is a sectional view of another swabbing apparatus embodying
the invention before the swab has been bathed in a
culture-sustaining liquid; and
FIG. 13 is a fragmentary cross-section of a lower portion of the
apparatus shown in FIG. 12 after the swab has been bathed in a
culture-sustaining liquid.
DETAILED DESCRIPTION OF PREFERRED EMBODIMENTS
A swabbing apparatus 10 constructed in accordance with this
invention for use in obtaining cultures is enclosed within a
wrapper 12 comprising paper or the like as shown in FIG. 1. As
shown in FIG. 2, the apparatus 10, which has been removed from the
wrapper 12 of FIG. 1, comprises a container tube 14 and a closure
member or cap 16 which is not sealed to the upper and open end 18
of the tube 14. The tube 14 contains a culture-sustaining liquid 20
at the closed end or bottom 22 of the tube 14 and remains isolated
from a swab 24 comprising an elongated member or stick 26 and an
absorbent tip 28. This isolation is achieved by a piston-like plug
30 comprising a substantially resilient material such as rubber or
a plastic so as to resiliently and sealingly engage the walls of
the tube 14. The plug 30 includes a one-way valve 32 which allows
the liquid 20 to flow through an opening in the plug 30 when the
plug is forced downwardly into the liquid 20 but maintains
substantial physical isolation between the absorbent tip 28 of the
swab 24 and a culture-sustaining liquid when the plug 30 is in the
position shown in FIG. 2.
The tube 14 also contains an inner tube 34 on which the cap 16 (the
cap is shown as shortened in length because of space limitations of
the drawings) is resting which forms a chamber spaced and isolated
from the sides of the tube 14 and within which the swab 24 is
located. An absorbent material 36 such as cotton, rayon or a foam
is located within the chamber formed by the tube 34 supporting the
tip 28 above the plug 30.
Once the apparatus 10 has been removed from the wrapper 12 as shown
in FIG. 2, the cap 16 may be removed from the upper end of the
inner tube 34. A swab 24 may then be grasped between the finger
tips at the upper end protruding from the inner tube 34 and removed
from the tube 14. At that time, the absorbent tip 28 may be brought
into contact with that portion of the body from which a culture is
to be taken and re-inserted back into the inner tube 34.
At this time, the plug 30 is pushed downwards through the liquid 20
by pressing on the cap 16 which in turn presses on the upper end of
the inner tube 34 so as to transmit the downward force therethrough
to the plug 30 moving it to the position shown in FIG. 3. Note that
the level of the liquid 20 now extends above the bottom of the
inner tube 34 so as to saturate the absorbent material 36 and
thereby assure that the tip 28 remains moist to keep the culture
alive.
In order for the cap 16 to perform this pushing function with
respect to the plug 30, the inside of the cap has a particular
configuration. An elongated sealing surface 38 is provided which is
adapted to engage the outside of the container tube 14 so as to
isolate, at least to some degree, the contents of the tube once the
surface 38 has been brought into contact with the outside of the
tube 14. In addition, the cap 16 includes a shoulder which extends
substantially perpendicular to the axis of the cap 16 so as to
provide a surface which pushes against the upper end of the tube
34. Finally, the cap 16 includes a recess 40 which receives the
upper end of the swab stick 26. The recess 40 has slightly less
depth than the protrusion of the swab stick 26 beyond the end of
the tube 34 to assure the swab 24 is, to some degree, formed into
the cotton 36.
The nature of the one-way isolating valve in the plug 30 is shown
in some detail in the enlarged view of FIG. 2a. The valve 32
includes a hole 42 which extends upwardly from the underside of the
plug 30. An inclined slit is provided which extends from the upper
surface of the plug 30 at one side of the hole 42 down to the upper
end of the hole 42 so as to form a flap 46 which is biased to the
closed position when the plug 30 is stationary within the liquid 20
and pressure on opposite side of the plug 30 are substantially
equal. It will be understood that the bias provided by the
resilient material of the plug is sufficient to maintain the
resilient flap 46 closed even when there is some pressure
differential between opposite sides of the plug to be sure that the
valve remains closed even when the tube 14 is inverted. For other
details relating to the nature of this valve, reference is made to
the inventor's own U.S. Pat. No. 3,661,265.
Referring now to FIG. 4, a somewhat modified cap 48 is shown where
there is no recess to receive the upper end of the stick 26 which
protrudes beyond the upper end of the tube 34. In this embodiment,
the cap 48 forces the stick 26 down into the absorbent material 36
and also bends the stick 26. In this embodiment, it is generally
desirable to have the stick protrude somewhat less beyond the upper
end of the inner tube 34 so that the bending of the stick 26 and
the burying in the absorbent material 36 may accommodate the extra
length of the stick without undue pressure on the plug 30 which
might force the valve 32 to remain closed. However, because there
is some pressure on the cap 48, a rather tight seal must be
achieved between the sealing surface 38 and the outside walls of
the tube 14 in order to hold the cap 48 on the upper end of the
tube 14. Since such a tight fit will not permit the escape of the
air as the cap 48 is applied to the tube 14, a one-way air vent 50
is provided at the upper end of the sealing surface 38. The air
vent 50 is similar is design to the one-way isolating valve shown
in detail in FIG. 2a. In other words, it is designed to maintain
substantial isolation of the contents within the tube 14 while
opening under the influence of compressed air within the tube as
the cap 48 forms a seal therewith.
In the embodiment of FIGS. 2 and 3, an air valve similar to that
shown in FIG. 4 has not been provided. It is not of course
necessary as long as the skirt of the cap 16 having a sealing
surface 38 is sufficiently flexible to spread and thereby release
the air within the tube 14 when the cap 16 is applied to the tube
14. Of course, a one-way air valve could be provided in the cap 16
if the cap 16 was too rigid to permit the escape of air along the
sealing surface 38.
In accordance with another important aspect of the invention, the
culture-sustaining liquid 20 may be periodically introduced into
the area above the plug 30 so as to extend the period in which the
culture may be kept alive. For this purpose marks 52 shown in FIGS.
3 and 5 are provided on the exterior and upper end of the tube 14.
When the cap 16 is first placed on the tube 14 after the culture
has been taken, it is pushed down to the first of the marks 52. It
may then be sequentially advanced to the other marks at desirable
intervals, e.g., every 24 hours, to keep re-introducing the liquid
into contact with the absorbent material 36 and absorbent tip 28 of
the swab 24.
In the embodiment of FIG. 6, the inner tube for pushing the plug
downwardly through the liquid 20 is eliminated and the pushing is
performed by the swab 24 itself. For this purpose, a cap 54 is
provided with a skirt including an internal sealing surface 56 for
engaging the outside walls of the tube 14 and a recess comprising
upwardly and inwardly tapered walls 58 leading to a flat surface 60
near the top of the cap 54. The purpose of the taper 58 is to
provide a surface which will center the upper end of the stick 26
with respect to the cap 54 and thereby direct the forces applied to
the cap substantially axially downwardly through the tube 14 to
move a plug 62 through the liquid 20 as shown in FIG. 7. Without
the taper 58, the stick 26 may be off-center with respect to the
cap 54 and this would apply a non-axial force to the plug 62 with
the possibility of skewing it within the tube 14. Although this
skewing would still permit the liquid to saturate the absorbent
material 36 and the tip 28, it would not permit the controlled
periodic introduction of the liquid into contact with the tip 28 as
is considered highly desirable.
Note that plug 62 has a rather limited contact with the walls of
the tube 14 as achieved by the tapered surface 64 at the periphery
thereof. This reduces the amount of friction between the plug 62
and the walls of the tube 14 to permit the swab 24 to push the plug
downwardly through the liquid 20. It will of course be appreciated
that the strength of the stick 24 is substantially less than the
tube 34 shown in FIGS. 2 and 3. A valve 66 in the plug 62 is
substantially identical to that shown in FIG. 2a.
Note that the sealing surface 56 of the cap 54 does engage the top
of the tube 14 even without submerging the plug 62 in the liquid
20. This is desirable in that it does permit the wrapper 12 as
shown in FIG. 1 to be eliminated without contaminating the swab 24
or the interior of the tube 14 prior to use of the swab 24 to take
a culture.
In order to assure that the plug containing the one-way valve does
not become skewed within the tube 14 when the swab 24 depresses it
through the liquid 20, it is possible to provide an additional area
of contact between the tube 14 and the plug as shown in FIG. 8.
Plug 68 of FIG. 8 includes a tapered surface 70 with an area 72
adapted to resiliently and sealingly engage the walls of the tube
14. In addition, an upper sealing area 74 is provided along an
upper tapered surface 76 which also engages the sides of the tube
14 and serves to stablize the plug 68 within the tube 14 without
substantially increasing the friction encountered when attempting
to submerge the plug 68 in the liquid 20.
In order to prevent the tip of the swab from closing a one-way
isolating valve 78, the valve 78 is recessed at the botton of a
hole 80 extending downwardly from an upper surface 82. The hole 80
is sufficiently small to prevent the tip of the swab from forcing
the absorbent material down into the hole but sufficiently large to
allow the flap of the valve 78 which is similar to that shown in
FIG. 2a to open when the plug 68 is being submerged in the
culture-sustaining liquid.
In the embodiment of FIG. 9, the stick 26 of the swab 24 is
embedded or otherwise attached to a cap 84 having a centrally
located pedestal 86 and a skirt 88 providing a sealing surface for
engaging the tube 14 shown in phantom. The pedestal 86 is provided
so as to permit substantially the entire length of the swab stick
26 to be inserted into a particular body canal without contacting
the body canal with the skirt 88 of the cap 84. With this
arrangement, the problem of skewing the plug within the tube is
substantially eliminated since mounting of the swab 24 on the cap
84 at the central portion thereof substantially assures axially
directed forces on the center of the plug.
In the embodiment of FIGS. 10 and 11, the problem of axially
directing a force on the center of a plug has been solved by
providing a downwardly and inwardly tapering surface 90 in a plug
92 which, when utilized in conjunction with a cap 60 having an
upwardly and inwardly tapered surface 58 as shown in FIGS. 6 and 7,
will serve to center a freely movable swab 24 along the axis of the
tube thereby assuring the central, axially directed force on the
plug 92. In addition to centering the tip 28 of the swab 24 on the
plug 92, the tapered surface 90 also allows the swab 24 to assist
in opening spaced one-way valves 94 which are located near the
periphery of the plug 92. The valves 94 include elongated holes 96
extending substantially parallel with the axis of the plug and
terminated by flaps 98 which are formed in part by the tapered
surface 90. By observation it will be seen that any pressure
located in the central area of the surface 90 will tend to collapse
areas 100 of the plug beneath the flaps 98 so as to assist in
opening the valves 94. It will also be seen that at least one valve
94 will be clear of the tip 28 to permit it to open.
It is also possible to slightly modify the valves 94 which are
similar to that shown in FIG. 2a by providing an essentially
vertical slit at the top of the holes 96 extending upwardly from a
central position of the holes 96 rather than extending horizontally
from the side of the hole as shown in FIGS. 10 and 11. As a result,
there are two flaps formed instead of one. Such a valve may be
placed at the bottom of the tapered surface 90 with the result that
the swab pushes on the tapered surface above the valve so as to
assist in opening the valve as the plug is pushed toward the bottom
of the tube.
In order to reduce the amount of friction along the side of the
tube, sealing engagement is provided by annular ridges 102. The use
of a plurality of such ridges again serves to stablize the plug
without substantially increasing the friction.
In the embodiment of FIGS. 12 and 13, the movable plug has been
eliminated and the culture-sustaining liquid 20 has been
incapsulated within an ampoule 104 having a downwardly and inwardly
tapered or concave surface 106 at the upper end thereof. The
surface when used in combination with an upwardly tapered surface
58 of the cap 54 serves to center and axially direct the force
applied to the flap 54 thereby permitting the use of the swab 24 to
transmit a force sufficient to break as shown in FIG. 13, the
surface 106 which may comprise, for example, a plastic such as
polyethylene. The remainder of the ampoule 104 may comprise any
suitable material such as a plastic which may be fused or otherwise
sealingly affixed to the material forming the surface 106. It is
important in the embodiment of FIGS. 12 and 13 as well as FIGS. 10
and 11, not to use excessive amounts of absorbent material 36 since
large amounts of such material might impede the centering of the
swab 24 and the centering of the axially directed force applied by
the cap 54.
The culture-sustaining liquid 20 may comprise any liquid which is
capable of providing the culture with an environment in which it
can live. For this purpose, the liquid might comprise distilled
water, a saline solution or another non-reactive liquid capable of
sustaining such life.
Although specific embodiments of the invention and modifications
thereof have been described and suggested, it will be understood
that the appended claims are intended to cover other embodiments
and modifications which fall within the true spirit and scope of
the invention.
* * * * *