U.S. patent number 3,890,204 [Application Number 05/509,706] was granted by the patent office on 1975-06-17 for culture collecting package.
This patent grant is currently assigned to Marion Health and Safety, Inc.. Invention is credited to Carl Frederick Avery.
United States Patent |
3,890,204 |
Avery |
June 17, 1975 |
Culture collecting package
Abstract
A culture collecting swab and a sealed glass ampoule are
packaged side-by-side within an envelope. After a culture has been
collected on the swab, one end portion of the ampoule is broken off
to open the ampoule and permit insertion of the swab into the
ampoule and into contact with culture-sustaining media contained in
the ampoule.
Inventors: |
Avery; Carl Frederick
(Rockford, IL) |
Assignee: |
Marion Health and Safety, Inc.
(Rockford, IL)
|
Family
ID: |
24027780 |
Appl.
No.: |
05/509,706 |
Filed: |
September 26, 1974 |
Current U.S.
Class: |
600/572; 206/229;
206/361; 206/461; 435/30; 435/810 |
Current CPC
Class: |
C12M
33/02 (20130101); C12M 45/22 (20130101); A61B
10/0096 (20130101); A61B 2010/0216 (20130101); G01N
2001/028 (20130101); Y10S 435/81 (20130101) |
Current International
Class: |
C12M
1/26 (20060101); C12M 1/30 (20060101); G01N
1/02 (20060101); C12k 001/10 () |
Field of
Search: |
;195/127,139,109 ;23/23B
;128/2W |
References Cited
[Referenced By]
U.S. Patent Documents
Primary Examiner: Monacell; A. Louis
Assistant Examiner: Fan; C. A.
Attorney, Agent or Firm: Wolfe, Hubbard, Leydig, Voit &
Osann, Ltd.
Claims
I claim as my invention:
1. A culture collecting package, said package including a sealed
one-piece ampoule made of glass, said ampoule having an elongated
tubular body of circular cross-section and having domed ends, a
culture-sustaining media contained within said ampoule, and a
circumferentially extending score line formed in said body whereby
one end portion of the ampoule may be broken away from the other
end portion along said score line to allow access to said media,
said package further including a swab comprising an elongated stem,
a swabbing tip on one end of said stem, and a closure on the other
end of said stem and sized to telescope with and close the open end
of said other end portion of said ampoule after a culture has been
collected on said tip and the latter has been placed in contact
with said media.
2. A culture collecting package as defined in claim 1 including an
elongated envelope, said ampoule and said swab being disposed
side-by-side within said envelope prior to initial opening of the
envelope.
3. A culture collecting package as defined in claim 1 in which said
ampoule is filled with a gas which is heavier than air.
4. A culture collecting package as defined in claim 1 in which said
score line extends only part way around the circumference of said
body, and further including a flexible band wrapped around said
body and covering said score line, one end portion of said band
extending around one end portion of said ampoule and the other end
portion of said band extending around the other end portion of said
ampoule, at least one end portion of said band being secured to the
corresponding end portion of said ampoule, and a circumferentially
extending tear line formed around said band and alined axially with
said score line.
5. A culture collecting package, said package including a sealed
one-piece ampoule, said ampoule comprising an elongated tubular
body having closed ends, a culture-sustaining media contained
within said ampoule, and a circumferentially extending score line
formed in said body to enable breaking of one end portion of the
ampoule away from the other end portion to allow access to said
media, said package further including a swab comprising an
elongated stem, a swabbing tip on one end of said stem, and a
closure for closing the open end of said other end portion of said
ampoule after a culture has been collected on said tip and the
latter has been placed in said other end portion in contact with
said media.
Description
BACKGROUND OF THE INVENTION
This invention relates to devices used by physicians and the like
for collecting a culture from various areas of a patient's body and
for keeping the culture alive for a period of time after it has
been collected. Such devices customarily include an absorbent swab
upon which the culture is collected by swabbing a particular body
area, and further include a container having its own supply of
culture-sustaining media. After the culture has been collected, the
swab is placed into the container and in contact with the media so
as to keep the culture alive until it is subsequently tested.
Devices of this general type are disclosed in Pickering U.S. Pat.
No. 3,579,303; Aronoff, et al., U.S. Pat. No. 3,773,035;
Henshilwood, et al., U.S. Pat. No. 3,783,104 and Henshilwood U.S.
Pat. No. 3,783,106.
One of the most commercially successful culture collecting devices
is of the type disclosed in Avery, et al., U.S. Pat. No. 3,450,129.
Such a device includes a flexible outer tube within which is
retained a frangible glass ampoule having a liquid
culture-sustaining media sealed therein. After a culture has been
collected on a swab, the latter is placed into the tube and the
tube is squeezed to break the ampoule and release the liquid. The
liquid moistens an absorbent plug which is disposed within the tube
in engagement with the tip of the swab so as to keep the latter
moist until the culture is tested.
A large part of the success of the Avery, et al., culture
collecting device is attributable to the fact that the
culture-sustaining media is originally sealed within an ampoule
made of glass. The glass ampoule can be easily and effectively
sterilized with steam and it does not react either with the
culture-sustaining media or the culture.
SUMMARY OF THE INVENTION
One of the primary aims of the present invention is to provide a
new and improved culture collecting device which retains all of the
basic advantages of the prior Avery, et al., device while being
simpler in construction, easier to use, and more readily adaptable
to collect and sustain different types of cultures.
A further object is to provide a comparatively simple and
inexpensive culture collecting device which may employ either a
liquid or solid culture-sustaining media and which is capable of
maintaining the culture in a substantially oxygen-free
atmosphere.
A more detailed object is to provide a culture collecting device
having a unique glass ampoule which not only holds the
culture-sustaining media but which also serves as a container for
the swab after the culture has been taken. By providing an ampoule
of this type, the need for an outer tube and an absorbent plug such
as used in the Avery, et al., device is eliminated completely and
yet the advantages accruing from the use of a glass ampoule are
retained.
The invention also resides in the novel construction of the ampoule
to facilitate the gaining of access to the culture-sustaining
media.
These and other objects and advantages of the present invention
will become more apparent from the following detailed description
when taken in conjunction with the accompanying drawings.
BRIEF DESCRIPTION OF THE DRAWINGS
FIG. 1 is a perspective view of a new and improved culture
collecting package embodying the novel features of the present
invention.
FIG. 2 is a perspective view of the ampoule.
FIG. 3 is a perspective view of the swab.
FIG. 4 is a cross-section taken along the line 4--4 of FIG. 3.
FIG. 5 is a perspective view showing the ampoule being opened to
enable access to the culture-sustaining media.
FIG. 6 is a perspective view showing the swab telescoped with the
ampoule.
DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENT
As shown in the drawings for purposes of illustration, the
invention is embodied in a package 10 whose components are used for
collecting a bacterial specimen or culture from a patient's body
and for maintaining the culture in a live condition until such time
as it can be tested. The present invention contemplates the
provision of a culture collecting package having new and improved
components which are simpler, less expensive and easier to use than
those available heretofore and which are capable of maintaining the
collected culture in either a normal or an oxygen-free atmosphere
while preserving the culture in either a liquid or solid
culture-sustaining media.
More specifically, the package 10 includes a swab 11 and an ampoule
13 which initially are disposed side-by-side within an elongated
and substantially flat envelope 14. The latter comprises a flexible
transparent blister 15 which is releasably sealed to a peel-away
backing 16. After the swab and the ampoule have been sealed in the
envelope, the envelope may be autoclaved in order to sterilize the
swab and the outside of the ampoule. The swab and the ampoule may
be removed from the envelope by peeling away one end portion of the
backing 16 and taking the swab and ampoule out of the opened end of
the envelope.
The swab 11 comprises an elongated stem 17 (FIG. 3) made of plastic
and having an absorbent swabbing tip 19 such as cotton on one end
thereof. After a culture has been collected on the tip, it is
necessary to place the tip in contact with a culture-sustaining
media 20 in order to keep the culture in a live condition until it
reaches a laboratory for testing.
In carrying out the invention, the culture-sustaining media 20 is
originally sealed within the ampoule 13 which advantageously is
made of glass and which serves as a container for the swab 11 after
the culture has been collected. As shown in FIG. 2, the ampoule is
of one-piece construction and includes an elongated tubular body 21
having a circular cross-section and having ends which are defined
by generally hemi-spherical domes 23 and 24. The culture-sustaining
media 20 is disposed within one end portion of the ampoule and, in
this instance, is shown as being a liquid such as modified Stuart's
transport media. The culture-sustaining media can, however, be a
solid substance such as an agar slant which may be located in the
end portion of the ampoule either with or without a charcoal
filtering substance or other filtering agent. Some cultures can
survive and grow only in an oxygen-free atmosphere and thus, in
some instances, the ampoule also will be filled with a
non-oxidizing and preferably heavier-than-air gas such as
nitrogen.
After the culture has been collected on the swabbing tip 19, the
ampoule 13 is opened to permit the tip to be placed in contact with
the media 20. For this purpose, a circumferentially extending score
line 25 (FIG. 2) is scratched in the outside of the ampoule body 21
adjacent the dome 24, the score line being comparatively short and
extending only part way around the circumference of the body. When
the ampoule is held as shown in FIG. 5, one end portion of the
ampoule may be snapped cleanly away from the other end portion at
the break point defined by the short score line 25. After the
ampoule has been thus opened, the broken away end portion is
discarded and the swab stem 17 is inserted into the longer end
portion to place the tip 19 into contact with the media 20 (see
FIG. 6). To inform the user as to where to break the ampoule, a
band of tape 26 (FIG. 2) with printed instructions may be wrapped
around the body 21, the tape having end portions overlapping the
end portions of the ampoule and having a perforated tear line 27
alined with the score line 25.
As an incident to inserting the swab stem 17 into the ampoule 13,
the open end of the ampoule is closed to keep contaminants out of
the ampoule and to prevent the nitrogen or the like from escaping
from the ampoule. For this purpose, a closure or cap 30 (FIG. 3) is
joined to the end of the stem 17 opposite the tip 19 and is sized
to telescope tightly over the open end portion of the ampoule. The
cap is made of plastic and includes a series of axially extending
ribs 31 (FIG. 4) which are spaced circumferentially around the
interior of the cap and which tightly grip the swab stem 17. If the
cap is placed quickly on the ampoule just after the latter has been
opened, the relatively heavy nitrogen will not escape from the
ampoule and no substantial amount of air will enter the ampoule.
After being closed by the cap 30, the ampoule 11 may be replaced in
the envelope 14 and delivered to the laboratory for testing of the
culture on the swab tip 19.
Formation of the ampoule 11 is effected much in the same way as the
ampoule disclosed in the aforementioned Avery, et al., patent. That
is, one end portion of the body 21 is flamed over to form the dome
23 and then the culture-sustaining media 20 is inserted into the
ampoule along with a non-oxidizing gas if the latter is desired.
Thereafter, the other end portion of the body is flamed over to
form the dome 24 and seal the media within the ampoule. Filling and
sealing of the ampoule are effected under sterile conditions and
thus the interior of the ampoule is sterile when the ampoule is
packaged in the envelope 14.
From the foregoing, it will be apparent that the present invention
brings to the art a new and improved culture collecting device
which possesses the basic advantages of the commercially successful
Avery, et al., device presently being marketed and provides several
additional advantages. That is, the new device does not require an
outer plastic tube nor an absorbent plug. There is no danger of
glass fragments from a crushed ampoule collecting on the swab tip
or piercing through an outer tube. The new device is capable of
using both solid and liquid culture-sustaining media and of
maintaining the culture in an oxygen-free atmosphere. Moreover, the
cap 30 may be made shorter since the swab 11 assumes only a single
position within the ampoule 13 rather than a pre-collecting
position and a different post-collecting position as in the case of
the swab within the outer tube of the prior Avery, et al.,
device.
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