U.S. patent number 3,888,972 [Application Number 05/252,462] was granted by the patent office on 1975-06-10 for process for preparation of wide-pore adsorbent for use in chromatography.
Invention is credited to Andrei Vladimirovich Kiselev, Galina Lvovna Kustova, Boris Alexandrovich Lipkind, Jury Stepanovich Nikitin.
United States Patent |
3,888,972 |
Kiselev , et al. |
June 10, 1975 |
Process for preparation of wide-pore adsorbent for use in
chromatography
Abstract
A process for preparing a wide-pore adsorbent adapted for use in
chromatography, comprising mixing finely dispersed non-porous
silica having a specific surface area S of 35 to 380 m.sup.2 /g
with water followed by drying the aqueous suspension at a
temperature within the range of 100 to 450.degree.C to give a
silica xerogel and particulating thereof. The xerogel, prior to
particulation or thereafter, is subjected to the hydrothermal
treatment in an autoclave at a temperature within the range of from
100 to 380.degree.C and under a pressure of 1 to 300 atm., followed
by drying at a temperature of 100 to 300.degree.C. The
above-described process makes possible the preparation of an
adsorbent with larger pores as compared to adsorbents prepared from
finely dispersed non-porous silica and produced by conventional
processes. The process according to the present invention makes it
possible to produce an adsorbent having a specific surface area
within the range of from 2 to 280 m.sup.2 /g and an average pore
diameter of from 230 to 30,000 A, while retaining a high pore
volume of 0.9 to 1.9 cm.sup.3 /g. Such adsorbent is useful in
chromatography for the purposes of separation, purification and
analysis of a wide range of substances: from low- and
moderately-boiling compounds up to polymers, and more particularly,
biopolymers, and viruses.
Inventors: |
Kiselev; Andrei Vladimirovich
(Moscow, SU), Kustova; Galina Lvovna (Gorky,
SU), Lipkind; Boris Alexandrovich (Gorky,
SU), Nikitin; Jury Stepanovich (Moscow,
SU) |
Family
ID: |
22956102 |
Appl.
No.: |
05/252,462 |
Filed: |
May 11, 1972 |
Current U.S.
Class: |
502/407;
423/335 |
Current CPC
Class: |
B01J
20/103 (20130101); C01B 33/16 (20130101) |
Current International
Class: |
B01J
20/10 (20060101); C01B 33/00 (20060101); C01B
33/16 (20060101); C01b 033/16 (); C01b
033/18 () |
Field of
Search: |
;423/338,339,335
;23/293A ;252/449,451 |
References Cited
[Referenced By]
U.S. Patent Documents
Other References
Book, "The Colloid Chemistry of Silica and Silicates," by Ralph K.
Iler, 1955 Ed., pages 137 and 138. Cornell Univ. Press, Ithaca, New
York..
|
Primary Examiner: Stern; Edward
Claims
We claim:
1. A process of preparing a porous silica adsorbent adapted for use
in chromatography comprising the steps of (1) mixing finely
dispersed non-porous silica having a specific surface area of 35 to
380 m.sup.2 /g with water to form an aqueous suspension; (2) spray
drying the suspension at a temperature of 400.degree. to
450.degree.C. to form a particulated silica xerogel; (3)
hydrothermally treating the xerogel at a temperature of 100.degree.
to 380.degree.C. and a pressure of 1 to 300 atm.; and (4) drying
the treated xerogel at a temperature of 100.degree. to
300.degree.C.
2. A process according to claim 1 further comprising the step of
calcining the silica xerogel in an atmosphere of steam or air at a
temperature of from 550.degree. to 1000.degree.C. after either or
both of steps (2) and (4).
Description
The present invention relates to processes for the production of a
wide-pore adsorbent adapted for use in chromatography from a finely
dispersed non-porous silica having a specific surface area of 35 to
380 m.sup.2 /g. Such an adsorbent in useful as an active packing in
chromatographic columns and as a support of stationary phases in
gas and liquid chromatography, as well as a molecular sieve in gel-
permeation chromatography.
A process is known for preparing a wide-pore adsorbent comprising
mixing a finely dispersed non-porous silica having a specific
surface area S of 170 or 280 m.sup.2 /g with water, followed by
drying the suspension at a temperature of 100.degree. to
450.degree.C to produce a silica xerogel. Said xerogel is then
subjected to grinding and screening (cf. N. K. Bebris, A. V.
Kiselev, Y. S. Nikitin "Kolloidnyj zhournal" 29, No. 3, 326, 1967;
N. K. Bebris et al. "Neftechimia" journal, 8, No. 3, 481,
1968).
The adsorbent produced by the known process has a specific surface
area of 160 or 260 m.sup.2 /g and an average pore diameter of 550
and 230 A respectively.
Said prior-art process has a disadvantage in that it is impossible
to increase the pore size of the resulting adsorbent within broad
limits, thereby restricting its applicability in chromatography.
Thus, for the analysis of high-molecular substances by methods of
gel-permeation chromatography a range of adsorbent with various
pore sizes is required including those with pores larger than in
the adsorbent produced by the known process.
Another disadvantage of said process resides in great losses of the
adsorbent during grinding thereof to particles less than 0.05 mm in
size (up to 15 percent by weight of the starting silica).
Still another disadvantage of the said process resides in the
irregular shape of the particles resulting from grinding, whereby
the uniformity of distribution of the chromatographic column
packing particles and hydrodynamic properties of the packing are
impaired.
It is an object of the present invention to eliminate the aforesaid
disadvantages.
More particularly, it is an object of the present invention to
change the manner of treatment of a xerogel produced by drying an
aqueous suspension of a finely dispersed non-porous silica having a
specific surface area S of 35 to 380 m.sup.2 /g at a temperature of
100 to 450.degree.C in order to prepare an adsorbent possessing the
necessary structural characteristics and reduce its losses during
the preparation thereof.
According to the present invention, said object has been
accomplished by subjecting the xerogel of silica prior to
particulation or thereafter to hydrothermal treatment in an
autoclave at a temperature of 100.degree. to 380.degree. C and
under a pressure of 1 to 300 atm., followed by drying thereof at a
temperature of 100.degree. to 300.degree.C.
The process of the present invention enables the preparation of an
adsorbent with larger pores and smaller specific surface as
compared to the adsorbent produced by the known process. By the
hydrothermal treatment of the xerogel produced from non-porous
silica it is possible to prepare an adsorbent having a specific
surface area of from 2 to 280 m.sup.2 /g and an average pore
diameter of from 230 to 30,000 A while retaining a high pore volume
of from 0.9 to 1.9 cm.sup.3 /g. Such an adsorbent is useful in
chromatography for separation, purification, and analysis of a wide
range of substances: from low- and moderately-boiling substances up
to polymers, more particularly biopolymers, and viruses.
As has been mentioned above, the hydrothermal treatment of the
silica xerogel may be effected both prior to and after the
particulation of the xerogel. In doing so, the particulation of the
xerogel is performed by grinding thereof.
In addition, in case of hydrothermal treatment of the xerogel
followed by the drying thereof after the particulation procedure,
it is advisable to effect said procedure simultaneously with drying
the silica suspension by spraying thereof in a drying oven at a
temperature of 400.degree.-450.degree.C. As a result of such
combination of stages (suspension drying and xerogel particulation)
the resulting xerogel is in the form of spherical particles which
are much less liable to destruction in the autoclave during the
hydrothermal treatment, this being especially important under high
pressure and temperature treatment conditions. In addition, drying
the suspension and particulation of xerogel under the
above-specified conditions enables the obviation of xerogel
grinding and is conducive to a reduction of adsorbent losses to as
low as 5 to 7 percent by weight of the starting silica. The
production of the adsorbent in the form of spherical particles also
provides conditions for improving the uniformity of distribution of
the chromatographic column packing particles and hydrodynamic
properties of the packing.
For making the adsorbent applicable within a wide temperature
range, for removing bound water therefrom and for increasing the
adsorbent strength, it is advisable, prior to the hydrothermal
treatment and/or after the hydrothermal treatment and drying of the
silica xerogel, to calcine said xerogel at a temperature of
550.degree. to 1,000.degree.C in air or in a steam atmosphere.
The process for the preparation of a wide-pore adsorbent from
finely dispersed non-porous silica and adapted for use in
chromatography, according to the present invention, is effected in
the following manner.
1 part by weight of finely dispersed silica (such as aerosil, white
soot) is mixed with 1.6-12 parts by weight of water till a
suspension is formed which is dried at a temperature of 100.degree.
to 450.degree.C. The resulting silica xerogel is placed into an
autoclave, mixed with water and subjected to hydrothermal treatment
at a temperature of 100.degree. to 380.degree.C under a pressure of
1 to 300 atm. during a period of 4 to 100 hours. After the
hydrothermal treatment the xerogel is dried at a temperature of
100.degree. to 300.degree.C. The resulting adsorbent is then ground
and screened into fractions. Grinding may also be performed prior
to the hydrothermal treatment of the xerogel (after drying the
aqueous suspension of silica).
To produce a wide-pore adsorbent in the form of spherical particles
and reduce the adsorbent losses it is advisable to perform drying
of the aqueous suspension of silica and the particulation of
xerogel simultaneously by spraying said suspension in a drying oven
at a temperature of 400.degree. to 450.degree.C. The resulting
xerogel particles have a spherical form and their size ranges from
0.05 to 1 mm. Then the hydrothermal treatment of the xerogel and
its drying are effected is accordance with the above-described
procedure, whereafter the adsorbent is ready for use.
As it has been mentioned hereinbefore, in order to make the
adsorbent applicable within a wider temperature range, to remove
bound water therefrom and to increase the adsorbent strength, it is
advisable, prior to the hydrothermal treatment and/or after the
hydrothermal treatment and drying of the xerogel of silica, to
calcine said xerogel at a temperature of 550.degree. to
1,000.degree.C in air or in a steam atmosphere during a period of 6
to 24 hours.
For a better understanding of the present invention the following
examples of the preparation of a wide-pore adsorbent from a fine
disperse non-porous silica are given by way of illustration.
EXAMPLE 1
280 g of finely dispersed non-porous silica having a specific
surface area S of 175 m.sup.2 /g are mixed with one liter of water
to yield a homogeneous suspension which is then dried at a
temperature of 140.degree.C. The resulting xerogel is divided into
three samples, each of them is subjected to hydrothermal treatment
in an autoclave under the following conditions: sample I, at
100.degree.C and 1 atm. pressure for 69 hours; sample II, at
275.degree.C and 60 atm. for 4 hours; sample III, at 380.degree.C
and 300 atm. for 4 hours. Then the samples are dried in a drying
cabinet at 200.degree.C, ground and screened to separate the
0.05-0.8 mm fraction.
The yield of the final product for each of the samples is about 85
percent by weight of the starting silica.
The structural characteristics of the adsorbents produced from the
three xerogel samples are given in Table 1 below.
Table 1
__________________________________________________________________________
Structural characteris- Adsorbent Adsorbent Adsorbent tics of the
resulting from from from adsorbent Sample I Sample II Sample III
__________________________________________________________________________
Specific surface area, S m.sup.2 /g 135 48 4 Average pore diameter,
d, A 560 1,600 19,500 Pore volume, V, cm.sup.3 /g 1.9 1.9 1.9
__________________________________________________________________________
EXAMPLE 2
Into an impeller 60 kg of finely dispersed non-porous silica having
a specific surface area of 380 m.sup.2 /g are added along with 490
l of water and thoroughly intermixed. The resulting suspension is
dried in a spraying furnace at a temperature of 450.degree.C with a
simultaneous particulation of the xerogel. 62 kg of the
particulated xerogel featuring spherical particles of 0.05 to 0.8
mm in size and humidity of 5 percent is subjected to hydrothermal
treatment in an autoclave at a temperature of 220.degree.C under 20
atm. pressure for 24 hours. Then the xerogel is dried at
300.degree.C for 8 hours, whereafter it is screened to obtain the
0.05 to 0.8 mm fraction.
The yield of the desired product is 57.9 kg or 96.5 percent by
weight of the starting silica. The adsorbent has the following
structural characteristics: specific surface area S =66 m.sup.2 /g;
average pore diameter d = 940 A; pore volume V = 1.55 cm.sup.3
/g.
EXAMPLE 3
280 g of finely dispersed non-porous silica having a specific
surface area S of 160 m.sup.2 /g are mixed with one liter of water
to yield a homogeneous suspension which is dried at a temperature
of 200.degree.C. The resulting xerogel is ground and screened to
separate the 0.05 to 0.5 mm fraction and calcined in air at
750.degree.C for 10 hours. The calcined xerogel is subjected to
hydrothermal treatment in an autoclave at a temperature of
250.degree.C under a pressure of 40 atm. for a period of 4 hours.
Then the xerogel is dried at 200.degree.C and screened to separate
the 0.05 to 0.5 mm fraction.
The yield of the final adsorbent is 92 percent by weight of the
starting silica. The adsorbent has the following characteristics:
specific surface area S = 33 m.sup.2 /g; average pore diameter d =
1,650 A; pore volume V = 1.37 cm.sup.3 /g.
EXAMPLE 4
280 g of finely dispersed non-porous silica having a specific
surface area S of 175 m.sup.2 /g are mixed with one liter of water
to produce a homogeneous suspension which is dried at a temperature
of 140.degree.C. The resulting xerogel is hydrothermally treated in
an autoclave at a temperature of 275.degree.C and under a pressure
of 60 atm. for four hours. Thereafter the xerogel is dried at
200.degree.C and divided into two samples. Sample 1 is calcined in
air at 1,000.degree.C for 6 hours, while sample 2 is calcinated in
an atmosphere of steam at 750.degree.C for six hours. The calcined
samples are ground and screened to separate the 0.05 to 0.8 mm
fraction.
The yield of the final product for each of the samples is 84
percent by weight of the starting silica.
The structural characteristics of the adsorbent prepared from the
two xerogel samples are given in Table 2 below.
Table 2 ______________________________________ Structural
characteristics Adsorbent Adsorbent of the resulting adsorbent from
from Sample 1 Sample 2 ______________________________________
Specific surface area S, m.sup.2 /g 45 47 Average pore diameter, d,
A 1,600 1,620 Pore volume V, cm.sup.3 /g 1.8 1.9
______________________________________ Said calcination of samples
results in essential reduction of the bound water content W
(.mu.mol H.sub.2 O/g of adsorbent) and hydroxyl group concentration
on the adsorbent surface .alpha..sub.OH (mcmol OH/m.sup.2 of
adsorbent surface). Thus, the adsorbent before the calcination has:
W = 630 and .alpha..sub.OH = 8.0; Sample 1 has: W = 30 and
.alpha..sub.OH .notident. 1.3; Sample 2 has: W = 190 and
.alpha..sub.OH = 5.7. The calcination enhanced the adsorbent
strength. The losses at vibration abrasion of the adsorbent prior
to the calcination are 4.5 percent by weight, while those of the
calcined samples 1 and 2 are 2.5 and 1.8 percent by weight
respectively.
EXAMPLE 5
Into an impeller 60 kg of finely dispersed non-porous silica having
a specific surface area S of 380 m.sup.2 /g and 490 l of water are
charged and thoroughly intermixed. The resulting suspension is
dried in a spraying furnace at a temperature of 450.degree.C with a
simultaneous particulation of the xerogel. 62 kg of the
particulated xerogel featuring spherical particles 0.05-0.8 mm in
size and 5 percent humidity are subjected to hydrothermal treatment
in an autoclave at a temperature of 220.degree.C and under a
pressure of 20 atm, for a period of 24 hours. Then the xerogel is
dried at 120.degree.C and calcined at 900.degree.C in air for a
period of six hours. After the calcination the adsorbent is
screened to separate the 0.05-0.8 mm fraction.
The yield of the final product is 57.6 kg or 96 percent by weight
of the initial non-porous silica. The structural characteristics of
the adsorbent are as follows. Specific surface area S = 64 m.sup.2
/g; average pore diameter d = 950 A; pore volume V = 1.52 cm.sup.3
/g.
EXAMPLE 6
Into an impeller 60 kg of finely dispersed non-porous silica having
a specific surface area S of 175 m.sup.2 /g and 430 l of water are
charged and thoroughly intermixed. The resulting suspension is
dried in a spraying furnace at a temperature of 400.degree.C with
simultaneous particulation of the xerogel. The particulated xerogel
featuring spherical particles of 0.1 to 1 mm in size is divided
into three samples; each of them is hydrothermally treated under
the following conditions. Sample 1, 220.degree.C and 20 atm. for 96
hours; Sample 2, 280.degree.C and 100 atm. for 16 hours; Sample 3,
370.degree.C and 290 atm. for 6 hours. The samples are then dried
at a temperature of 100.degree.C and calcined in air at
900.degree.C over a period of 24 hours. After the calcination the
samples are screened to separate the 0.1 to 1 mm fraction.
The yield of the final product produced from Sample 1 is 96
percent; Sample 2, 94.8 percent; Sample 3, 93 percent by weight of
the starting silica.
The structural characteristics of the adsorbents produced from the
three samples of the xerogel are given in Table below.
Table 3 ______________________________________ Structural
character- Adsorbent Adsorbent Adsorbent tics of the resulting from
from from adsorbent Sample 1 Sample 2 Sample 3
______________________________________ Specific surface area 46 16
2.2 S,m.sup.2 /g Average pore diameter d, 1,600 4,400 30,000 Pore
volume V, cm.sup.3 /g 1.81 1.77 1.74
______________________________________
EXAMPLE 7
45 g of finely dispersed non-porous silica having a specific
surface area S of 35 m.sup.2 /g are mixed with 100 ml of water. The
resulting aqueous suspension is dried at a temperature of
100.degree.C in a drying cabinet. The xerogel thus obtained is
hydrothermally treated at 100.degree.C and under a pressure of 3
atm. for a period of 4 hours. Then the xerogel is dried at
300.degree.C and calcined in air at 750.degree.C for eight hours.
After the calcination the adsorbent is ground and screened to
separate the 0.05 to 0.5 mm fraction.
The yield of the final product is 84 percent by weight of the
starting silica. The adsorbent has the following structural
characteristics: specific surface area S = 29 m.sup.2 /g; average
pore diameter d = 1,250 A; pore volume V = 0.92 cm.sup.3 /g.
EXAMPLE 8
Into an impeller 60 kg of finely dispersed non-ferrous silica
having a specific surface area S of 175 m.sup.2 /g are charged
along with 430 l of water and thoroughly intermixed. The resulting
suspension is dried in a spraying furnace at a temperature of
400.degree.C with a simultaneous particulation of xerogel. The
particulated xerogel featuring spherical particles of 0.1-1 mm in
size and 5 percent humidity is calcined in air at a temperature of
550.degree.C for a period of eight hours. The calcinated xerogel is
then hydrothermally treated in an autoclave at a temperature of
220.degree.C and under 20 atm. pressure for a period of 96 hours.
Thereafter, the xerogel is dried at 120.degree.C and calcined in
the air at 900.degree.C for 24 hours. After the calcination the
adsorbent is screened to separate the 0.1 to 1 mm fraction.
The yield of the final product is 98 percent by weight of the
starting silica. The adsorbent has the following structural
characteristics: specific surface area S = 44 m.sup.2 /g average
pore diameter d = 1,500 A; pore volume V = 1.68 cm.sup.3 /g.
EXAMPLE 9
Into an apparatus impeller 60 kg of finely dispersed silica having
a specific surface area S of 175 m.sup.2 /g are charged along with
450 l of water and thoroughly intermixed. The resulting suspension
is dried in a spraying furnace at a temperature of 450.degree.C
with simultaneous particulation of xerogel. 62 kg of the
particulated xerogel featuring spherical particles of 0.1 to 1 mm
in size and 5 percent moisture content are calcined in an
atmosphere of steam at 850.degree.C for a period of six hours.
The calcinated xerogel is hydrothermally treated at a temperature
of 115.degree.C and under a pressure of 1.5 atm. for a period of 4
hours. Then the xerogel is dried at 300.degree.C and screened to
separate the 0.1-1 mm fraction.
The yield of the final product is 97.5 percent by weight of the
starting silica. The adsorbent has the following structural
characteristics: specific surface area S = 85 m.sup.2 /g, average
pore diameter d 32 700 A; pore volume V = 1.52 cm.sup.3 /g.
* * * * *