U.S. patent number 3,881,527 [Application Number 05/430,384] was granted by the patent office on 1975-05-06 for bulb-operated pipet.
Invention is credited to Justin Joel Shapiro.
United States Patent |
3,881,527 |
Shapiro |
May 6, 1975 |
Bulb-operated pipet
Abstract
A liquid transfer pipet consisting of a tubular barrel having an
inlet conduit portion at one end and a reduced stem at the other
end having a capillary bore. An apertured operating bulb is
slidably and sealingly engaged on the stem. The capillary bore acts
to allow filling of the barrel but substantially prevents liquid
from entering the bulb because it allows the operator time to
remove his finger from the vent aperture of the bulb. The capillary
bore is of sufficiently small diameter to provide stable retention
of liquid contained in the barrel. The operating bulb may form part
of the cap or stopper of a bottle, vial, or similar vessel. The
capillary bore may be formed merely in a solid top extension of the
pipet main barrel without requiring the formation of a reduced
stem.
Inventors: |
Shapiro; Justin Joel (Berkeley,
CA) |
Family
ID: |
23707338 |
Appl.
No.: |
05/430,384 |
Filed: |
January 3, 1974 |
Current U.S.
Class: |
141/24; 422/922;
222/420; 141/29 |
Current CPC
Class: |
B01L
3/0282 (20130101) |
Current International
Class: |
B01L
3/02 (20060101); B65b 003/04 () |
Field of
Search: |
;73/425.5P,425.6
;128/232,233 ;141/2,18,21-29,31,325-327 ;222/158,206,420 |
References Cited
[Referenced By]
U.S. Patent Documents
Primary Examiner: Aegerter; Richard E.
Assistant Examiner: Schmidt; Frederick R.
Attorney, Agent or Firm: Gordon; Herman L.
Claims
What is claimed is:
1. A liquid transfer pipet comprising a barrel member having a
liquid-receiving cavity portion and an intake conduit element at
one end of said barrell member communicating with said cavity
portion, the opposite portion of said barrel member being formed
with a capillary bore coextensive in length therewith in
communication with said cavity portion and opening at the opposite
end of the barrel member, and a flexible operating bulb sealingly
engaged on and slidably receiving said opposite portion, said bulb
being formed with an upper vent opening adapted to be sealingly
engaged at times by an operator, and wherein said opposite portion
of the barrel member is of substantial length and of uniform
cross-sectional size and shape and is much smaller than the bulb,
whereby the bulb may be adjusted to a position along said opposite
portion wherein said opposite portion projects upwardly a
substantial distance through an opening in the bulb to define
within said bulb above said opposite portion an air space and
within said bulb below said opposite portion an overflow space of
substantial volume in the bottom of the bulb around said opposite
portion, and said upper vent opening being in communication with
said air space, and whereby liquid accumulating in said overflow
space will not re-enter said capillary bore when the bulb is
squeezed to discharge liquid from the pipet.
2. The liquid transfer pipet of claim 1, and wherein said capillary
bore has an inside diameter of between 0.002 and 0.02 inch.
3. The liquid transfer pipet of claim 1, and wherein the end of
said opposite portion received in the operating bulb is tapered,
whereby to minimize the possibility of re-entry of overflow liquid
from the bulb into the capillary bore when liquid is discharged
from the pipet.
4. The liquid transfer pipet of claim 1, and wherein said opposite
portion is in the form of a reduced stem of substantially uniform
cross-sectional size, whereby the operating bulb may be at times
adjusted in position along said reduced stem.
5. The liquid transfer pipet of claim 1, and wherein the capillary
bore is sufficiently small in inside diameter and of sufficient
length to develop the capillary sealing action required to support
the total volume of liquid receivable in said cavity.
6. The liquid transfer pipet of claim 1, and wherein said opposite
portion is in the form of a reduced stem of circular crosssectional
shape.
Description
This invention relates to liquid transfer devices, and more
particularly to a pipet of the bulb-operated type.
A main object of the invention is to provide a novel and improved
bulb-operated liquid transfer pipet which is very simple in
construction, which is easy to operate, and which is volumetrically
precise.
A further object of the invention is to provide an improved
bulb-operated pipet which is versatile in use, which is easy to
control, and which acts to automatically substantially terminate
liquid intake action as soon as its barrel is completely filled
with liquid, the intake action being terminated by the resistance
to flow of a capillary column, the resistance to flow being
sufficient to give the operator time to discontinue the application
of filling pressure.
A still further object of the invention is to provide an improved
bulb-operated liquid transfer pipet which is compossed of
relatively inexpensive and easily manufacturable parts, which
extracts highly accurate measured volumes from liquid containers,
and which allows said measured volumes to be safely and reliably
transferred without loss.
A still further object of the invention is to provide an improved
liquid transfer pipet of the bulb-operated type which is
constructed so that substantially all of its liquid retention
chamber is visible to the operator and so that he can quickly
terminate filling pressure when the chamber is filled.
A still further object of the invention is to provide an improved
liquid pipet, which once filled can be placed in any position or
handled fairly roughly without losing its contents.
A still further object of the invention is to provide an improved
pipet closure for containers of diagnostic test reagents.
A still further object of the invention is to provide an improved
bulb-operated liquid transfer pipet wherein the operating bulb is
combined with a closure cap for a container and wherein the bulb
and cap are adjustable so that they can be at times shifted to a
position allowing the pipet to reach a bottom corner portion of the
associated container, whereby by tilting the container practically
all the liquid in the container may be ultimately removed, thus
minimizing waste of the liquid.
A still further object of the invention is to provide an improved
bulb-operated pipet which cuts off filling pressure when its main
chamber is filled and reduces the possibility of drawing liquid
into its operating bulb, the time period required for filling the
main chamber being a definite value so that the operator can
terminate the filling pressure at the end of such time period,
making it unnecessary for him to closely watch the chamber during
the filling operation, the pipet being thereby usable with high
speed and precision while requiring a minimum amount of visual and
mechanical effort and requiring a relatively low degree of
dexterity on the part of the operator.
Further objects and advantages of the invention will become
apparent from the following description and claims, and from the
accompanying drawings, wherein:
FIG. 1 is a vertical cross-sectional view taken through a typical
bulb-operated liquid transfer pipet constructed in accordance with
the present invention.
FIG. 2 is a vertical cross-sectional view of a modified form of
pipet in accordance with the present invention, shown combined with
a screw cap closure on a liquid container.
FIG. 3 is a vertical cross-sectional view of the pipet of FIG. 2,
shown with its operating bulb and screw cap in an extended position
enabling the pipet to reach residual liquid in a bottom corner
portion of the associated container, with the container tilted.
FIG. 4 is a vertical cross-sectional view of the pipet of FIG. 2,
shown combined with the ground glass closure stopper of a reagent
bottle.
FIG. 5 is a vertical cross-sectional view of the pipet of FIG. 2,
shown combined with the rubber stopper of a laboratory vial.
FIG. 6 is a fragmentary vertical cross-sectional view showing a
further modification of a bulb-operated liquid transfer pipet
according to the present invention.
Referring to the drawings, and more particularly to FIG. 1, 11
generally designates a typical liquid transfer pipet according to
the present invention. The pipet comprises a tubular main barrel 12
having a reduced bottom inlet conduit portion 13 integrally
connected to the main barrel 12 by a tapered portion 14. Main
barrel 12 is provided with a reduced top stem 15 of substantial
length having a capillary bore 16 which may be from about 0.002 to
0.02 inch in inside diameter. Slidably and sealingly mounted on
stem 16 is a filling bulb 17 of resilient deformable material, such
as rubber or the like. Bulb 17 is provided with a top vent aperture
18 adapted to be at times sealingly covered by the user's finger,
for example, after the bulb has been squeezed to a collapsed
condition and it is desired to draw liquid into barrel 12, or when
it is desired to expel liquid from the barrel after it has been
filled.
The barrel 12 has a generally frusto-conical top wall 19, with the
stem 15 integrally formed at its apex. When liquid drawn into the
barrel 12 reaches top wall 19, it is thereafter forced into the
capillary bore 16, which imposes a high resistance to flow and
thereby substantially abruptly terminates intake of liquid into the
barrel. With a given pipet, the filling time will therefore be
substantially constant, and an operator can quickly learn to remove
his finger from the aperture 18 at the end of the filling time
period after a few experimental usages of the pipet. Alternatively,
the operator can easily visually determine when the liquid reaches
top wall 19. In either case, the intake flow is so retarded by the
action of the capillary bore 16 that the operator has sufficient
time to remove his finger from aperture 18 after barrel 12 is
filled to prevent liquid from being forced completely through and
beyond the capillary bore 16.
After filling, the surface tension of the liquid in the capillary
bore 16 acts in a manner to cause this liquid to seal the bore and
to thereby allow the pipet to be transported without loss of liquid
from the filled barrel 12. The liquid can be thereafter discharged
from the pipet by sealing vent aperture 18 with the user's finger
and applying squeezing pressure to the bulb 17.
In the event that during filling the operator fails to release his
finger from the vent aperture 18 in time to prevent liquid from
overflowing from the top end of capillary bore 16 into the bulb 17,
it will not re-enter the bore when the pipet is discharged in the
above-described normal manner since it will tend to accumulate at
the bottom of the bulb around the stem 15. The possibility of such
overflow can be minimized by making stem 15 of substantial length,
for example, from about 3/4 inch to as much as 2-1/2 inches. To
further reduce the possibility of re-entry of overflow liquid from
bulb 17 into the capillary bore when liquid is discharged from the
pipet, the top end of the capillary stem portion may be made
conical, as shown at 21 in FIGS. 2 to 5, or frusto-conical, as
shown at 22 in FIG. 6.
As shown in FIG. 6, the capillary bore 16 may be formed merely in a
solid top extension 20 of the pipet main barrel, wherein no
reduction in diameter of the top portion of the main barrel body is
necessary.
Although the capillary bore 16 is of substantial length, its inside
diameter is so small that its entire volume, compared to the
measured volume in the main barrel of the pipet, is not large
enough to cause any significant error in the measured volume of
liquid dispensed by the pipet.
Referring now to FIGS. 2 and 3, a pipet 23 generally similar to the
previously described pipet 11 is shown combined with a screw cap
closure 24 threadedly engageable on the neck 25 of a conventional
bottle 26. In this embodiment the operating bulb, shown at 27, is
integrally formed with a generally frusto-conical annular bottom
sealing stopper 28 which is received beneath an inturned annular
top lip or flange 29 of the generally ring-shaped rigid screw cap
24, so that when the cap 24 is tightened the stopper element is
clamped sealingly on the top rim of the bottle neck 25. The top
stem, shown at 15', is slidably and sealingly engaged through the
thickened bottom portion 30 of the bulb 27 concentrically with
stopper 28. With the screw cap 24 loosened sufficiently to allow
atmospheric air to enter the bottle through the neck 25, the pipet
assembly can be employed in the same manner as previously described
to dispense accurately measured liquid volumes from the bottle 26.
To remove practically all of the liquid from the bottle, so as to
minimize waste, the cap 24 may be unscrewed from the neck of the
bottle and the bulb 27 can be adjusted upwardly on the stem 15', as
shown in FIG. 3, whereby the inlet conduit portion 13 can be
manoeuvered to reach the lowermost corner portion of the bottle, in
the tilted position of the bottle illustrated, whereby to draw the
last remaining amounts of liquid into the pipet.
In the embodiment illustrated in FIG. 4, the pipet assembly of FIG.
2 is combined with a stopper plug 31 similar to the ground glass
sealing plug of a reagent bottle 32. The stopper plug 31 is of
hollow construction and has a top wall which is centrally apertured
to receive a rubber, or similar resilient deformable grommet 33,
and the stem portion 15' of the pipet assembly is slidably and
loosely engaged through said grommet and the pipet assembly may be
employed in the same manner as described above in connection with
FIGS. 2 and 3 to dispense accurately measured volumes of liquid
reagent from the bottle 32.
In the embodiment illustrated in FIG. 5, the pipet assembly of FIG.
2 is employed with a centrally apertured rubber or similar stopper
plug 34 similar to the stopper plug of a conventional serum vial
35. The barrel 12 of the pipet assembly is loosely engaged through
the central aperture of the plug 34, and the pipet assembly may be
employed in the same manner as described above in connection with
FIGS. 1, 2 and 4.
In each of the embodiments illustrated in the drawings and
described above, the capillary bore at the top end of the pipet
barrel serves to 1) inhibit further entry of liquid into the barrel
when the measuring cavity therein has been completely filled, and
thereby give the operator time to remove his finger from the vent
aperture 18, and 2) provide a retention means, namely, the
capillary column of liquid in the bore, to enable the filled pipet
to be safely transported without risk of loss of its contents,
since the capillary column provides a holding effect sufficient to
support the liquid in the filled pipet barrel. In pipets of the
type previously employed, either of the mouth-operated or
bulb-operated type, the operator must place and hold his finger in
sealing engagement with the top end of the pipet or other vent
aperture of the pipet when the pipet is to be transported, calling
for considerable dexterity on the part of the operator. This is not
necessary with the above-described apparatus.
In operating any of the above-described forms of the present
invention, the operating bulb is first squeezed to a collapsed
condition, after which, with the intake end of the pipet immersed
in the liquid to be dispensed, the operator covers aperture 18 with
his finger and allows the bulb to resume its normal shape. This
draws liquid into the pipet barrel, filling the pipet barrel
cavity, the liquid rising therein until it enters the capillary
bore 15, at which time the filling action is abruptly terminated by
the resistance imposed by the capillary bore, as above described.
The operator then removes his finger from aperture 18 and the pipet
can then be transported to the intended receiving vessel or
receptacle, into which the liquid in the pipet may be then
discharged by again covering the aperture 18 and squeezing the
operating bulb.
In the various different forms of the invention above-described the
portion of the barrel carrying the operating bulb is preferably of
cylindrical shape and of uniform cross-sectional diameter, whereby
to facilitate adjustment of the bulb therealong as required.
While certain specific embodiments of liquid transfer pipets have
been disclosed in the foregoing description it will be understood
that various modifications within the spirit of the invention may
occur to those skilled in the art. Therefore it is intended that no
limitations be placed on the invention except as defined by the
scope of the appended claims.
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