U.S. patent number 3,881,464 [Application Number 05/311,817] was granted by the patent office on 1975-05-06 for sampling device and method.
Invention is credited to Max Moses Levene.
United States Patent |
3,881,464 |
Levene |
May 6, 1975 |
SAMPLING DEVICE AND METHOD
Abstract
A device for obtaining cell and tissue samples, for example for
screening for cervical cancer, which comprises a generally conical
brush with bristles soluble in a liquid that does not damage the
tissues, and a method of using the device.
Inventors: |
Levene; Max Moses (Kensington,
London S.W. 7, EN) |
Family
ID: |
10478871 |
Appl.
No.: |
05/311,817 |
Filed: |
December 4, 1972 |
Foreign Application Priority Data
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|
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Dec 9, 1971 [GB] |
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57304/71 |
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Current U.S.
Class: |
600/569; 604/1;
604/540; 15/207.2 |
Current CPC
Class: |
A61B
10/0291 (20130101); A61B 2010/0216 (20130101); A61B
2017/320012 (20130101) |
Current International
Class: |
A61B
10/00 (20060101); A61B 17/32 (20060101); A61b
010/00 () |
Field of
Search: |
;128/2B,2W,2R,269,304
;15/145,160,159A |
References Cited
[Referenced By]
U.S. Patent Documents
Primary Examiner: Howell; Kyle L.
Attorney, Agent or Firm: Brisebois & Kruger
Claims
I claim:
1. A method of obtaining from the uterine cervix a sample that is
suitable for both cytological and histological examination, which
comprises the steps of obtaining from the uterine cervix a sample
which contains both tissue and cellular material by contacting said
cervix with a generally conical brush having radially projecting
bristles made of soft fibres having the firm sample-retaining
texture of cellulose acetate, which bristles are soluble in a
solvent that does not cause morphological damage to the sample when
said bristles and sample are immersed in said solvent, but are
insoluble in conventional fixatives for said cells and tissues,
immersing the brush having the sample thereon in a fixative for
said sample, causing relative agitation between said brush and
fixative to remove cellular material therefrom for cytological
examination, and subsequently dissolving said bristles in said
solvent to prepare a sample suitable for histological
examination.
2. A method as claimed in claim 1 in which said fibres are made of
cellulose acetate.
3. A device for obtaining cell and tissue samples which comprises a
generally frusto-conical brush having a coaxial handle projecting
from its larger end and soft radially projecting bristles of
cellulose acetate which are soluble in a liquid that does not cause
morphological damage to the sample when said bristles and sample
are immersed in said liquid, the narrower end of said brush being
surmounted by a portion of substantially constant diameter.
Description
This invention relates to a process and device for collecting
specimens for diagnostic purposes.
While there has been as yet little progress in developing a cure
for various forms of cancer, in the sense of providing
pharmaceutical treatment, advances have been made in surgical
treatments which rely to a large extent for success on early
diagnosis. One example is in uterine cervical cancer where progress
has been made by instituting tests of the section of the population
at most risk. It is hoped that in future all women will be examined
at regular intervals in an attempt to attain early detection of
presence of abnormal cells.
At present, between 3 percent and 5 percent of women initially
subjected to cytological examination appear to have abnormal cells
and have to be examined by histological methods before a firm
diagnosis can be made. Many of those so examined are found not to
show symptoms of cancer. Apart from the expense and labor involved,
the apprehension naturally felt by those called back makes it
desirable to avoid a second examination. The presently used methods
of obtaining samples normally do not remove tissue, or sufficient
tissue, from the cervix to enable a histological test to be carried
out using the original sample. Further, the area of the cervix from
which the sample is collected does not usually extend sufficiently
to ensure that abnormal material is collected if it is indeed
present.
It is an object of the present invention to provide a device and
method whereby sufficient material can be collected at one time to
enable both cytological and histological examination to be carried
out if necessary.
Accordingly, the present invention provides a device for obtaining
cell and tissue samples which comprises a generally conical brush,
the bristles of which are soluble in a liquid that does not cause
morphological damage to the samples.
Further, the present invention provides a method of obtaining a
sample suitable for both cytological and histological examination
comprising contacting such a brush with a hollow viscus, for
example the uterine cervix, to obtain the sample on the bristles.
The bristles may then be contacted with such a liquid, and the
liquid containing the sample treated to prepare the sample in a
form suitable for at least one of the examinations.
The precise shape and size of the brush will, of course, be
determined by the degree of penetration and extent of contact
required in a particular case. By the use of a conical brush, of
the order of 2.5 to 4 cm height and 2 to 3 cm diameter at base, a
large surface area can be contacted at the desired regions.
It will be apparent that the bristles should normally be made of a
material that can be sterilized by generally used techniques, as
should any support needed for the bristles. It will also be
apparent that the brush should be made of materials that are not
harmful to the person being examined or to the samples
themselves.
Suitable materials include cellulose-based materials, for example
Courtauld "Dicel Celafibre," which are spun cellulose acetate
fibres. Such a brush is of soft but firm texture and is readily
soluble in acetone.
The brush is preferably attached to a sterilizable handle from
which it may be readily detached. Conveniently, the handle is a
straight rod or shaft of length about 12 to 18 cm, having at the
end to which the brush is to be attached a gripper mechanism for
detachably retaining the brush. At this end there is also
preferably provided a generally disk-shaped support member of
approximately the same diameter as the base of the brush and
slightly concave toward the brush.
Any suitable gripper mechanism may be used, for example a
spring-loaded chuck. This can be operated from the other end of the
handle whereby contamination is prevented. Alternatively, the brush
may be a force fit in or on the handle.
The invention will now be described by way of example only with
reference to the accompanying drawing.
Referring to the drawing:
A metal wire 1, comprising a pair of strands twisted together, acts
as a support to bristles (indicated generally by the reference
numeral 2), the construction being akin to that of a pipecleaner;
the bristles 2 comprise a region 3 of approximately constant
diameter extending from one end of the wire 1 to a point about a
third of the way along its length; the second region of bristles 4
extends from this point to the other end and is flared outwardly
from this point to form a frusto-cone the maximum diameter of which
is about 5 times that of the bristles in the region 3. Attached to
the end of the wire carrying the widest bristles is a rod 5 which
serves as a handle. The rod 5 has at the attached end a gripper 6
in the form of a chuck which is operated by a plunger 7 at the
other end of the rod. A generally saucer-like support 8 is
positioned on the attached end of the rod with its concave side
facing the bristles.
In use, the device comprising the brush and the handle is
sterilized, the plunger 7 is depressed to open the gripper 6 over
the wire 1 and the plunger 7 is then released. The brush is then
inserted into the cervix and rotated to obtain the sample. The
brush is then released from the gripper into a fixative solution.
The fixative solution is then shaken to cause enough cells to be
shed from the bristles to enable cytologic preparations to be made
for cytological diagnosis. If necessary or desired, the remaining
tissue and any remaining cells can be removed from the brush by
dissolving it in a suitable solvent to prepare samples for
histological diagnosis.
Suitable dimensions for the various parts are as follows:
wire 1 -- 3 cm in length; bristle region 3 -- 1 cm in length and
0.5 cm diameter; bristle region 4 -- 2 cm in length increasing in
diameter from 0.5 to 2.5 cm; support 8 -- 2.5 cm diameter; rod 5 --
15 cm in length.
* * * * *