U.S. patent number 3,848,581 [Application Number 05/296,215] was granted by the patent office on 1974-11-19 for apparatus for taking multiple samples of biological liquid.
Invention is credited to Louise Cinqualbre, nee Monier, Paul Cinqualbre.
United States Patent |
3,848,581 |
Cinqualbre , et al. |
November 19, 1974 |
APPARATUS FOR TAKING MULTIPLE SAMPLES OF BIOLOGICAL LIQUID
Abstract
Apparatus for taking samples of biological liquid and in
particular human blood comprises an elongate body having a
longitudinally extending central passageway connected at one end to
a hypodermic needle. Branch channels extend respectively from the
central passageway to individual test tubes which are supported by
their upper ends along the lower side of the body. The test tubes
are sealed so as to remain in sterile condition and means is
provided for selectively venting air from the test tubes and
controlling flow of liquid from the central passageway to the
individual test tubes so that they can be filled successively or as
desired. The test tubes are removable from the body for carrying
out the several tests or analyses for which the samples are
intended.
Inventors: |
Cinqualbre; Paul (Nancy,
FR), Cinqualbre, nee Monier; Louise (Nancy,
FR) |
Family
ID: |
26216654 |
Appl.
No.: |
05/296,215 |
Filed: |
October 10, 1972 |
Foreign Application Priority Data
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|
|
|
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Oct 8, 1971 [FR] |
|
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71.36314 |
Oct 2, 1972 [FR] |
|
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72.35882 |
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Current U.S.
Class: |
600/575;
600/579 |
Current CPC
Class: |
A61B
5/150236 (20130101); A61B 5/150244 (20130101); A61B
5/150213 (20130101); A61B 5/150389 (20130101); A61B
5/150519 (20130101); A61B 5/150221 (20130101); A61B
5/153 (20130101); A61B 5/150755 (20130101); A61B
5/15003 (20130101); A61B 5/150251 (20130101); G01N
1/14 (20130101); A61B 5/150259 (20130101) |
Current International
Class: |
A61B
5/155 (20060101); A61B 5/15 (20060101); G01N
1/14 (20060101); A61b 005/14 () |
Field of
Search: |
;128/2F,276,214.2,2G,214B,DIG.5,239,240,247,220,272,277,274,275
;141/236,8,363,351,318,193,288 ;73/425.4,423A ;137/588,256,259
;251/343 |
References Cited
[Referenced By]
U.S. Patent Documents
Primary Examiner: Gaudet; Richard A.
Assistant Examiner: Recla; Henry J.
Attorney, Agent or Firm: Burns; Robert E. Lobato; Emmanuel
J. Adams; Bruce L.
Claims
What we claim and desire to secure by letters patent is:
1. Apparatus for taking samples of a biological liquid and
particularly human blood to obtain a plurality of separate samples
for different analyses, comprising an elongated body having a
longitudinally extending central passageway, a hypodermic needle
connected with said central passageway at one end of said body, a
plurality of test tubes, means for removably mounting said test
tubes by their upper ends in depending position under said body
with the upper ends of the test tubes closed by said body, said
mounting means comprising means for slidably gripping the upper end
of each test tube for movement between an upper position and a
lower position and venting means comprising a vent passage which is
closed by the test tube when in its upper position and opened when
the test tube is in its lower position, and branch passageways
leading from said central passagewy to said test tubes
respectively, whereby the individual test tubes can be filled
successively to the desired extent with samples of liquid supplied
through said needle, central passageway and the respective branch
passageways by selectively manually lowering individual test tubes
to corresponding lower positions on said mounting means to allow
displacement of air in the lowered test tubes by fluid.
2. Apparatus according to claim 1, in which said branch passageways
are of such small cross section that flow of liquid therethrough
prevents the escape of air from the test tubes through said branch
passageways.
3. Apparatus according to claim 1, in which said mounting means
comprises a plurality of recesses in the lower side of said body,
each recess receiving the upper end of one of said test tubes.
4. Apparatus for taking samples of a biological liquid and
particularly human blood to obtain a plurality of separate samples
for different analyses, comprising an elongated body having a
longitudinally extending central passageway, a hypodermic needle
connected with said central passageway at one end of said body, a
plurality of test tubes, means for removably mounting said test
tubes by their upper ends in depending position under said body
with the upper ends of the test tubes closed by said body, said
mounting means comprising means for slidably gripping the upper end
of each test tube for movement between an upper position and a
lower position and venting means comprising a vent passage which is
closed by the test tube when in its upper position and opened when
the test tube is in its lower position, branch passageways leading
from said central passageway to said test tubes respectively and a
plunger longitudinally slidable in said central passageway to
selected positions to admit a flow of liquid from the central
passageway through the branch passageways to the respective test
tubes, whereby the individual test tubes can be filled successively
to the desired extent with samples of liquid supplied through said
needle, central passageway and the respective branch
passageways.
5. Apparatus according to claim 4, in which said branch passageways
are of such small cross section that flow of liquid therethrough
prevents the escape of air from the test tubes through said branch
passageways.
6. Apparatus according to claim 4, in which said mounting means
comprises a plurality of tubular projections projecting down from
the lower side of said body, each of said projections fitting into
the upper end of one of said test tubes.
7. Apparatus according to claim 6, in which a circular groove in
said body around the upper end of each of said projections receives
the upper edge of each of said test tubes to provide a seal.
8. Apparatus according to claim 6, in which each of said
projections has a circular external rib near its lower end and an
axially extending groove in its outer face extending from the lower
end of the projection to a point part way up the projection.
Description
FIELD OF INVENTION
The present invention relates to apparatus for the selective
sampling of biological liquid and more particularly blood and
especially human blood with a view to obtaining by a single
sampling a plurality of separate samples or specimens intended for
different tests or analyses.
BACKGROUND OF INVENTION
At present blood samples are customarily taken by means of a large
capacity syringe. In order to carry out different biological
analyses it is desirable to have some samples without coagulants
and others with coagulants or different preservatives. This
operation is still frequently effected in an archaic manner by a
single operation comprising traditional withdrawal of a sample by a
hypodermic syringe and its division in apparatus difficult to
protect from outside contaminants and under conditions disagreeable
for the patient.
SUMMARY OF THE INVENTION
The apparatus in accordance with the present invention avoids these
difficulties and objections by delivering samples of the blood or
other biological liquid through conduits to individual test tubes
for receiving samples for the several tests or analyses to be
performed. The samples are taken under conditions in which they are
protected from all outside contamination. Moreover, the apparatus,
by avoiding choice of anticoagulants by the operator, makes it
possible for the sampling to be carried out by persons who are not
highly specialized or skilled.
The invention accordingly provides apparatus for taking samples of
biological liquid from an organism and more particularly samples of
blood from a patient with a view to making a plurality of analyses
of such liquid characterized in that it comprises an elongate body
which is traversed longitudinally by a main canal connected at one
of its extremities to a hypodermic needle. The body is provided on
the side which is normally down during use, with a plurality of
individual means for holding test tubes by their upper extremities.
The main canal has branches leading to the individual test tubes
and means for directing the biological liquid into the test tubes
to fill them. Moreover, means which preferably controllable is
provided to permit the escape of air initially contained in the
test tubes as the test tubes are being filled.
The invention thus permits in a single sampling operation the
successive filling of each of the test tubes. When an upstream test
tube has been filled to the desired amount, it is sufficient for
the operator merely to actuate the respective closing means to
disconnect it from the main channel whereupon the liquid is
directed to a downstream test tube of which the individual closing
means is in open position.
Before the sampling, certain of the test tubes contain reagents for
analysis which are not required for the test being made, can be
disconnected from the circuit so that only those test tubes
corresponding to the desired analyses are maintained in position to
receive samples and are successively disconnected from the circuit
when the quantity of liquid in them is considered sufficient.
All manipulation of multiple test tubes which are delicate for the
operator and dangerous for the subject is thus avoided. The
individual test tubes used in the apparatus of the present
invention are placed on the support and are maintained in a sterile
condition up to and during the sampling.
Other characteristics and advantages of the invention will appear
from the following description of three preferred embodiments of
the invention which are shown by way of nonlimiting example in the
accompanying drawings in which:
FIG. 1 is a longitudinal section of apparatus in accordance with
the invention;
FIG. 2 is a cross section taken on the line II--II of FIG. 1;
FIG. 3 is a cross section identical with that of FIG. 2 but showing
the test tube in active position to receive a sample;
FIG. 4 is a longitudinal section of a second form of apparatus in
accordance with the invention;
FIG. 5 is a perspective view showing in detail a valve member of
the embodiment of FIG. 4;
FIG. 6 is a longitudinal section of a third embodiment of sampling
apparatus in accordance with the present invention;
FIG. 7 is a cross section of the apparatus along the line VII--VII
in FIG. 6; and,
FIG. 8 is a side elevation of a valve member of the apparatus of
FIG. 6.
The sampling apparatus shown by way of example in FIGS. 1, 2 and 3
comprises an elongated body 1 formed for example of metal or
plastic and having a longitudinally extending main canal 2
communicating at one end with a hypodermic needle 3 either directly
as shown in the drawings or by a flexible conduit. As seen in FIGS.
2 and 3 the body portion 1 is generally rectangular in cross
section. On its lower side the body 1 is provided with a plurality
of recesses 4 adapted to receive individual test tubes 5 which are
held by their upper ends in the recesses.
Each of the recesses 4 communicates with the main canal 2 by a
branch channel 6. The cross section of the branch channels 6 is
such as not to permit the counter-current passage of air from the
test tube when the test tube is being filled with the liquid being
sampled. To permit the escape of air, each of the recesses 4 is
provided with a lateral passage 7 which extends through to a side
of the body 1 so as to communicate with the atmosphere. To control
the flow of liquid into the test tube, each of the test tubes 5 is
movable in the respective recess 4 between an upper position as
shown in FIG. 2 and a lower position as shown in FIG. 3. When the
test tube is in the upper position as shown in FIG. 4, it closes
the air passage 7 so that air is not permitted to escape from the
test tube and hence liquid cannot enter the test tube through the
branch channel 6. When the test tube 5 is in the lower position as
illustrated in FIG. 3, air is permitted to escape through the
lateral passage 7 and hence liquid can enter the test tube through
the branch channel 6.
Prior to the taking of a sample, the body 1, hypodermic needle 3
and test tubes 5 are sterilized. The test tubes are assembled in
the body 1, preferably in their upper position as shown in FIGS. 1
and 2. They are thus maintained in sterile condition. Moreover, the
test tubes may contain, prior to sampling, the appropriate reagents
and notably the anticoagulants necessary in the case of apparatus
used for sampling blood. When the hypodermic needle has been
inserted into the subject, the blood or other liquid being sampled
flows through the hypodermic needle 3 into the main channel 1 and
from the main channel through a branch channel into the respective
test tubes. The test tubes are preferably filled successively.
Thus, the test tube nearest the needle is first positioned in the
lower position shown in FIG. 3 so as to receive liquid from the
main channel 1 through the corresponding branch channel 6. When a
sufficient amount of liquid has been received in the first test
tube, it is moved to its upper position as shown in FIG. 2 so as to
block the air escape passage 7 and thereby prevent further liquid
from entering the test tube. The liquid is thereupon directed to
the branch passageway of the next downstream test tube positioned
in its lower position to receive liquid. The test tubes may thus be
filled one after the other to the desired extent. The test tubes
are suitably numbered or otherwise identified so that each can be
designated for a particular test or analysis. If a particular test
is not to be made on the liquid being sampled, the corresponding
test tube can be left in its upper closed position so that it does
not receive liquid. The upper ends of the test tubes fit
frictionally in the respective recesses 4 of the body 1 so as to be
held securely in the recesses and to remain in the position to
which they are moved in controlling the flow of liquid into the
respective test tube. When the sampling has been completed, the
entire apparatus is taken to the laboratory where the test tubes
can be removed and appropriate analyses or tests are carried out on
the individual samples thus collected.
The embodiment of apparatus shown in FIG. 4 likewise comprises an
elongate body 1 having a longitudinally extending main canal 2
which is of capillary cross sectional dimensions. The body 1 is
also provided at its lower side with a plurality of recesses 4 for
receiving the upper ends of test tubes 5. Each of the recesses 4
communicates with the main canal 2 by a transverse channel 8 which
is perpendicular to the main canal and extends all of the way from
the recess 4 to the upper side of the body 1. Moreover, each of the
recesses 4 is provided with an air escape passage 9 of small cross
section which extends from the top of the recess into the main
canal 2 at a point downstream of the corresponding transverse
channel 8.
The test tube 5 is filled by liquid flowing down from the main
canal 2 through the transverse channel 8 while the air escapes
through the small passage 9. Communication of the main channel 2
with the atmosphere is provided by an auxiliary transverse passage
10 which is located at the end of the main canal 2 remote from the
hypodermic needle 3 and opens to the upper face of the rectilinear
body 1.
Each of the secondary transverse channels 8 is provided with a
valve member 11 controlling the flow of liquid into the
corresponding test tube. The valve member 11 is shown as comprising
a valve stem 11a which has a sliding close fit in the corresponding
passage 8 and is provided with an operating button 11b at its upper
end. Each valve stem 11a is provided with a transverse opening or
window 12 which is preferably circular. The valve member is movable
longitudinally between a lower closed position in which a lower
portion of the valve stem 11a closes the passage 8 leading from the
main canal 2 to the respective test tube holding recess 4 and an
open position in which the lower end of the valve stem is above the
main canal 2 so that liquid can flow from the main canal 2 through
the transverse channel 8 into the respective test tube. When the
valve member is in its lower position, the window 12 is in
alignment with the main channel 2 and permits flow of liquid
through the window to a downstream test tube. The hole 12 in the
valve member 11 is preferably of a diameter equal to that of the
main canal 2. If desired, the valve member can be moved to an
intermediate position in which the valve stem blocks both the main
canal and also the branch channel to the respective test tube. This
might be done for example to assure that all of the liquid for the
time being goes to an upstream test tube.
The apparatus shown in FIGS. 4 and 5 is used in substantially the
same manner as described with respect to that shown in FIG. 1 to 3
except that flow of liquid to the individual test tubes is
controlled by means of the valve members 11 rather than by the
position of the test tubes, all of which remain seated in their
uppermost positions. As previously described, the test tubes can
contain suitable reagents as desired and can be filled one after
the other to the desired extent by suitable manipulation of the
respective valve members 11 which fit frictionally in the
respective channels so as to stay at the positions to which they
are moved. The test tubes can be filled in any desired order and
any of them can be left empty if it is so desired.
A third form of apparatus is shown in FIGS. 6, 7 and 8. In this
embodiment an elongate body 1 for supporting the test tubes 5 is
traversed longitudinally by a central conduit 13 which is open at
both ends. On opposite sides of the conduit 13 the upper portion of
the body 1 is provided with longitudinally extending parallel
recesses 14 and 15. A flexible conduit 16 at one end of the body 1
joins the central conduit 13 with a hypodermic needle 17.
On the side which normally faces downwardly when the apparatus is
in use the body 4 is provided with a plurality of downwardly
projecting cylindrical projections or tubular sleeve portions 18
which are of a size to fit into the upper end of and hold a test
tube 5. A circular groove 19 is provided in the lower face of the
body 1 around the upper portion of each of the cylindrical
projections 18 to receive the upper edge of the test tube and
permit a tight closing of the test tube. Communication between the
central conduit 13 and the interiors of the cylindrical projections
18 is effected by an opening 20 of a conical top portion 21 of the
recess formed inside of the cylindrical projection. Through this
opening 20 each of the tubular test tube holders 18 communicates
directly with the central conduit 13.
Each of the test tube holders 18 is provided near its lower end
with two outwardly projecting circumferential ribs 22 and 23 which
are spaced a short distance apart to provide an intervening groove
between them. At one side of the tubular part 18 there is provided
an axially extending groove 24 which is formed in the outer surface
of the tubular member and extends from its lower conical end
approximately halfway up the length of the tubular part. These
notches extend down to the lower end portions of the tubular
members 18 and are all disposed on the sides toward the needle 17
in the same longitudinal median plane of the apparatus.
The ribs 22 and 23 are in a position to guide the respective test
tube and to hold it in an upper position in which the upper edge of
the test tube is seated in the circular groove 19 or in a lower
position in which the upper part of the test tube uncovers an upper
portion of the groove 24. When the test tube is in its upper
position, the upper end of the test tube is closed and air cannot
escape from the interior of the test tube. There is thus formed a
cushion of air which prevents entry of liquid into the test tube.
When the test tube is in its lower position, the upper portion of
the groove 24 is uncovered so that air is permitted to escape from
the test tube and hence liquid can enter.
A sealing plunger 25 is slidable for its whole length in the
interior of the central conduit 13. The plunger is provided at
regular intervals throughout its length with circular ribs 25a
forming sealing rings which assure a good separation between the
test tubes and fluid tightness with respect to the atmosphere. The
spacing of the sealing rings 25 is equal to the spacing of the test
tubes along the body 1. The inner end of the plunger 25 has a
conical point 26 while the other end extends beyond the body 1 and
is provided with an enlarged head 27 which serves as a hand grip
for operation of the plunger.
The operation of the apparatus shown in FIGS. 6 to 8 is essentially
the same as that of the apparatus shown in the preceding figures.
With the first test tube (nearest the hypodermic needle) in its
lower position so that air can escape and with the plunger 25
positioned so that its inner end is between the entrances to the
first and second test tubes, liquid will flow from the central
conduit 13 into the first test tube. When this has been filled to
the desired amount it is raised to its upper closed position and
the plunger is withdrawn sufficiently to permit liquid to flow to a
downstream test tube. In this manner the test tubes can be filled
successively or otherwise as desired.
While the invention has been described with reference to the
preferred embodiments illustrated in the drawings it will be
understood that it is in no way limited to these embodiments and
that various modifications of form and material may be made. The
various features of the three embodiments shown in the drawings are
mutually interchangeable insofar as they are compatible and other
modifications may be made within the spirit of the invention.
* * * * *