U.S. patent number 3,819,106 [Application Number 05/243,347] was granted by the patent office on 1974-06-25 for sample bag.
Invention is credited to Samuel J. Schuster.
United States Patent |
3,819,106 |
Schuster |
June 25, 1974 |
SAMPLE BAG
Abstract
A pre-sterilizable sample bag is disclosed for receiving and
storing a sample for analysis at a location remote from the situs
of sample collection. The bag is fabricated of material essentially
completely impermeable to bacteria and other foreign matter and
includes an access opening covered by a removable closure membrane
essentially completely impermeable to bacteria and other foreign
matter but highly permeable, in comparison to said bag material, to
sterilizing vapor. The outer surface of the bag includes an
adhesive coating adapted to be engaged in sealing contact with the
area of the bag material about the access opening to completely
seal the opening after removal of the closure membrane and
insertion of the sample into the bag.
Inventors: |
Schuster; Samuel J. (Monterey
Park, CA) |
Family
ID: |
22918389 |
Appl.
No.: |
05/243,347 |
Filed: |
April 12, 1972 |
Current U.S.
Class: |
206/439; 383/89;
206/459.1; 383/35; 229/80 |
Current CPC
Class: |
B65D
33/20 (20130101); A61L 2/26 (20130101); A61B
10/0096 (20130101); A61B 2050/002 (20160201); A61B
2050/314 (20160201) |
Current International
Class: |
A61L
2/26 (20060101); A61B 10/00 (20060101); B65D
33/20 (20060101); B65D 33/18 (20060101); A61B
19/02 (20060101); A61B 19/00 (20060101); B65d
033/20 () |
Field of
Search: |
;229/62,80 ;206/63.2R
;150/3 |
References Cited
[Referenced By]
U.S. Patent Documents
Primary Examiner: Price; William I.
Assistant Examiner: Garbe; Stephen P.
Attorney, Agent or Firm: Fraser and Bogucki
Claims
What is claimed is:
1. A pre-sterilizable bag for receiving and storing a sample for
analysis at a location remote from the situs of sample collection,
said bag being fabricated of a material essentially completely
impermeable to bacteria and including an access opening covered by
a removable closure membrane essentially completely impermeable to
bacteria, but highly permeable, in comparison to said bag material,
to sterilizing vapor, the outer surface of said bag including
sealing means shaped and positioned to be engaged in sealing
contact with the area of said bag material about said access
opening to completely seal said opening after removal of said
closure membrane and insertion of said sample into said bag.
2. A bag, as defined in claim 1, in which said bag material is
transparent plastic and said closure membrane comprises a paper
strip removably attached to the outer surface of said bag about
said access opening.
3. A bag, as defined in claim 1, in which said sealing means and
access opening are in spaced relation, said bag being foldable
along a portion of said bag between said sealing means and said
access opening for bringing the portion s of said bag about said
access opening into sealing contact with said sealing means.
4. A bag, as defined in claim 3, in which said access opening
comprises an elongated, generally linear slit and said sealing
means includes a pressure-sensitive adhesive coating applied to
said bag as a strip substantially parallel to said access
opening.
5. A bag, as defined in claim 4, which includes indicia adapted to
be brought into registry upon folding of said bag to assist in the
positioning of said access opening relative to said adhesive
coating.
6. A bag, as defined in claim 5, in which said indicia includes a
pair of spaced, linear marks parallel to each other and said access
opening.
7. A pre-sterilizable bag for receiving and storing biological
samples for subsequent analysis, said bag being fabricated of a
material essentially completely impermeable to bacteria and
including an access opening covered by a closure membrane, said
membrane being essentially completely impermeable to bacteria but
highly permeable, in comparison to said bag material, to
sterilizing vapor, said bag further including an adhesive coating,
said closure membrane being removable and said bag being foldable
to bring the region about said access opening into sealing
engagement with said adhesive coating to seal said opening against
the entry into said bag of bacteria, foreign matter or the
like.
8. A bag, as defined in claim 7, in which said bag material is
transparent plastic and said closure membrane comprises a paper
strip removably attached to the outer surface said bag about said
access opening.
9. A bag, as defined in claim 7, in which said adhesive coating and
access opening are in spaced, parallel relation, said bag being
foldable along a portion thereof between said adhesive coating and
said access opening for bringing the portions of said bag about
said access opening into sealing contact with said adhesive
coating.
10. A bag, as defined in claim 9, in which said access opening is
defined by overlapping longitudinal edge portions of said bag and
said adhesive coating comprises a pressure-sensitive adhesive
applied to the outer surface of said bag as a strip substantially
parallel to said access opening.
11. A bag, as defined in claim 10, which includes indicia adapted
to be brought into registry upon folding of said bag to assist in
the positioning of said access opening relative to said adhesive
coating and insure complete sealing of said opening.
12. A bag, as defined in claim 11, in which said indicia comprises
a pair of spaced, parallel lines.
13. A flexible sample bag, capable of having its interior
sterilized by sterilizing vapor, comprising:
a heat sealable plastic sheet essentially completely impermeable to
bacteria, said sheet having longitudinal and transverse edges and
being folded along longitudinal fold lines, said longitudinal edges
defining a longitudinally extending access opening;
a removable closure membrane of a material essentially completely
impermeable to bacteria and highly permeable, in comparison to said
plastic sheet material, to sterilizing vapor, said membrane
extending the length of said bag and closing said access opening,
said membrane being secured to the exterior of said bag by a
longitudinally extending, continuous, pressure heat seal joinder
line on each side of said opening;
continuous, pressure heat seal joinder lines extending along said
transverse edges closing said bag and further uniting said strip to
said plastic sheet along transverse edges; and
a pressure-sensitive adhesive coating applied to a longitudinally
extending area of the exterior surface of said bag, said area lying
in spaced, parallel relation to said access opening, said region
being overlayed by a protective strip ending use of said bag
whereby the interior of said bag is sterilizable by a process
involving the exposure of said bag interior to a sterilizing vapor
passed through said membrane, said membrane being removable to
permit insertion of said sample into said bag through said access
opening, said bag being sealable by removing said protective strip
and folding said bag to bring the area of said bag about said
access opening into sealing contact with said pressure-sensitive
adhesive area.
14. A sample bag, as defined in claim 13, in which at least one of
said transverse heat seal joinder lines is spaced from the
corresponding transverse edge to define a pull tab on said
removable membrane.
15. A sample bag, as defined in claim 13, which includes spaced
indicia registrable upon folding of said bag to assist in the
positioning of said access opening relative to said
pressure-sensitive adhesive coating to insure complete sealing of
said access opening.
Description
FIELD OF THE INVENTION
The present invention relates generally to sample receptacles and
particularly to pre-sterilizable, readily openable sample bags
resealable to completely prevent the entry of foreign matter so as
to maintain the condition of said sample for subsequent
analysis.
BACKGROUND OF THE INVENTION
There are presently available various kinds of receptacles for
collecting biological, marine, agricultural and other samples for
analysis at a location remote from the situs of sample collection.
Such sampling, collection and analysis have always been of
substantial importance to a number of industries and government
agencies for monitoring water resources, crops, and so forth, and
is assuming even greater importance today in light of the
increasing emphasis of the study and preservation of environmental
and ecological parameters.
The sample that is obtained in the field must, of course, be
retained in a state in which introduction of extraneous factors at
the time of collection or during retention are completely avoided
or at least minimized. One way to accomplish this is to insert the
sample in a container or receptacle that has been previously
sterilized and to immediately reseal the receptacle in a manner to
preclude the entry of foreign matter which would introduce unknown
qualitative and quantitative variables into the subsequent
analysis. The object is to preserve the sample in a condition in
which its subsequent laboratory analysis will be meaningful insofar
as ascertaining accurately its state at the time of collection.
SUMMARY OF THE INVENTION
According to the present invention there is provided a sample
receptacle in the form of a flexible bag whose interior may be
readily pre-sterilized. The sample bag of the invention is further
characterized by the ability to maintain a sterilized condition
over a long period of time and by the ease with which it may be
opened, loaded and sealed.
In accordance with the broad structural aspects of the invention, a
bag is provided that is fabricated of a material essentially
completely impermeable to bacteria and which includes an access
opening covered by a removable closure membrane essentially
completely impermeable to bacteria but highly permeable, in
comparison to the bag material, to sterilizing vapor such as steam
or ethylene oxide. The outer surface of the bag includes sealing
means shaped and positioned to be engaged in sealing contact with
the portions of the bag material about the access opening so that
the bag may be completely sealed after removal of the closure
membrane and insertion of the sample.
In accordance with other, more specific aspects of the invention,
the bag is foldable along a portion thereof between the sealing
means and the access opening so that the parts of the bag about the
access opening may be brought into sealing contact with the sealing
means. Indicia, such as a pair of spaced lines printed on the bag,
may be provided to facilitate sealing of the bag. Upon folding of
the bag, such indicia are brought into registry to assist in the
positioning of the access slit relative to the sealing means so
that complete sealing of the access opening is assured.
The bag is preferably constructed of transparent, heat sealable
plastic such as polyethylene or polypropylene or the like and the
closure membrane typically comprises a paper tear strip removably
attached to the outer surface of the bag about the access
opening.
According to a more specific form of the invention, the bag may be
constructed along the lines disclosed in U. S. Pat. No. 3,472,369
issued Oct. 14, 1969 to the inventor of the present invention, and
entitled "Readily Opened Package For Storing Items In Bacteria-Free
Condition." Pursuant to that construction, certain features of
which are incorporated herein, the bag is fabricated of a heat
sealable plastic sheet essentially completely impermeable to
bacteria. The sheet has longitudinal and transverse edges and is
folded along longitudinal fold lines so that the longitudinal edges
define a longitudinally extending access opening. A removable
closure membrane of paper, for example, essentially completely
impermeable to bacteria but highly permeable, in comparison to the
plastic sheet, to sterilizing vapor, extends the length of the bag
and closes the access opening, the membrane being secured to the
exterior of the bag by longitudinal, continuous, pressure heat seal
joinder lines adjacent the access opening on each side thereof.
Continuous pressure heat seal joinder lines extend along the
transverse edges closing the bag and further uniting the membrane
to the plastic sheet along the transverse edges.
Pursuant to specific aspects of the present invention, a
pressure-sensitive adhesive is applied to a longitudinally
extending rectangular area of the exterior surface of the bag, the
area lying parallel to the access opening. The adhesive area is
overlaid with a protective paper strip pending use of the bag. The
interior of the bag is sterilized by any of the well-known
processes involving the exposure of the bag interior to a
sterilizing vapor passing through the strip. In use, the closure
membrane is removed permitting insertion of the sample into the bag
through the access opening, following which the bag is sealed by
removing the protective strip and folding the bag to bring the area
of the bag about the access opening into sealing contact with the
pressure-sensitive adhesive area.
BRIEF DESCRIPTION OF THE DRAWINGS
Further objects, advantages and feature of the present invention
will become readily apparent from a reading of the detailed
description in conjunction with the accompanying drawings, in
which:
FIG. 1 is a perspective view of a sample bag constructed pursuant
to the teachings of the present invention;
FIG. 2 is a cross-section view of the bag of FIG. 1 as seen along
2--2;
FIGS. 3, 4, 5 and 6 are perspective views of a sample bag according
to the present invention illustrating, in sequence, the manner in
which the bag is used; and
FIG. 7 is an enlarged cross-section view of the upper portion of
sample bag in the sealed configuration, the section being taken
along 7--7 in FIG. 6.
DETAILED DESCRIPTION
For purposes of illustrating the background of the invention and
the state of the art, the aforementioned U. S. Pat. No. 3,472,369
issued to the present inventor on Oct. 14, 1969 is incorporated
herein by reference.
At the outset, it is to be noted that in the accompanying drawings
the thicknesses of the plastic and paper sheet material and that of
the adhesive coating have been greatly exaggerated to clearly show
their interrelationship. Further, in the description that follows,
the terms "longitudinal" and "length" denote directions and
dimensions parallel with the access opening and closure membrane
directions and the terms "transverse" and "width" denote directions
and dimensions perpendicular to the longitudinal direction.
Referring now to the drawings, sample bags according to the present
invention comprise mainly a flexible container 10, a closure
membrane 12 and an adhesive coating 14 covered by a protective
strip 16. The container portion of the bag comprises a rectangular
sheet of plastic such as polyethylene having a thickness sufficient
for purposes of strength and durability and for rendering the
container essentially completely impermeable to bacteria and other
foreign matter. Heat sealable materials other than polyethylene,
may, of course, be used, such materials including polypropylene,
nylon, polybutylene, and so forth, but polyethylene has the added
desirable properties of being low in cost and easily processed for
purposes of fabrication of the sample bag.
The container 10 is preferably made of transparent stock to
facilitate identification of the contents of the bag once the
sample is enclosed but it will be appreciated that translucent or
even opaque plastic can be utilized if desired for particular
applications. Tinted plastics to selectively filter light or other
electromagnetic radiation may also be employed. Further, the
plastic sheet stock may be treated by embossing in accordance with
techniques well known in the art and further may be a composite
structure fabricated of two every thin sheets cohered together
mechanically. Such coherence can be enhanced by subjecting the
sheet stock to corona discharge as is well known in the art.
Containers according to the present invention may be readily formed
from a continuous sheet of material, the longitudinal edges 18 and
20 thereof being folded inwardly along spaced, parallel
longitudinal fold lines 22 and 24. Portions 26 and 28 of the sheet
adjacent the longitudinal edges 18 and 20 are brought into
overlapping relation, with the upwardly extending portion 28
overlying the downwardly extending portion 26. The overlapping
portions 26 and 28 of the container 10 define a longitudinal access
slit or opening 30 which for purposes of the present invention is
preferably disposed near the upper longitudinal fold line 22 of the
container.
The closure membrane 12 comprises a longitudinal strip bonded to
the container by parallel heat seal joinder lines 32 and 34. The
functions of the membrane 12 are to close the access opening 30 and
form a barrier to bacteria and other foreign matter while
simultaneously being highly permeable to sterilizing vapor, such as
steam or ethylene oxide, in comparison to the plastic container 10.
The longitudinal heat seal joinder lines 32 and 34 bracket the
overlapping portions 26 and 28 of the container 10. Although these
bonds are preferably and most easily obtained by heat sealing,
using spaced-apart heat seal sealer bars elevated to the required
heat sealing temperature, they may also be provided by adhesive or
other forms of chemical or mechanical bonding.
The closure membrane 12 may be made of conventional paper 3 to 7
mils thick. Paper is characterized by several important properties
for purposes of this invention. Unlike plastic, increasing the
thickness of paper does not substantially affect its permeability
to sterilizing vapors. On the other hand, the labyrinth, fibrous
structure of paper is such that it effectively prevents bacteria
from penetrating into the container 10.
The closure membrane 12 may be provided with a thin polyethylene
coating (not shown), such coating being in contact with the outer
surface of the container 10. The thickness of the polyethylene
coating would preferably be of the order of 1/2 mil which is
insufficnet to impede the passage of sterilizing vapor but
nevertheless provides a sufficient thickness to facilitate the heat
seal joinder of the closure membrane 12 and the container 10.
Preferably, however, the container 10 is treated by corona
discharge along the longitudinal heat seal areas so as to render
the described thin polyethylene coating unnecessary, the membrane
12 being thereby directly heat sealable to the container 10.
Many kinds of inexpensive paper may be employed for the closure
membrane but depending upon particular needs other materials, both
woven and nonwoven, that are impermeable to bacteria yet are
comparatively highly permeable to sterilizing vapors may be used.
These alternatives would include various paper-like materials such
as glass fiber products and "Tyvac," a spun polyolefin of the
Dupont Company, all of which are impermeable to bacteria but in
comparison to the plastic container 10 highly permeable to
sterlizing vapor.
As shown in FIG. 1, the longitudinal joinder lines 32 and 34 may be
terminated short of the end of the closure membrane 12 to define a
pull tab 36 which serves as a manual gripping edge and thereby
facilitates removal of the closure membrane. The membrane 12 may
also have transverse extensions 38 and 40 beyond the longitudinal
joinder lines 32 and 34 of sufficient width to provide a lateral
pull tab along each longitudinal side in addition to, or instead
of, the end tab 36. It is generally sufficient to provide a pull
tab of only so much width that it can be grasped with the tips of
the fingers. Where greater tearing forces are involved more area
may be provided for more secure gripping.
The transverse end edges of the bag are joined to each other and to
the closure membrane 12 by transverse joinder lines 42 and 44 each
of which is adjacent one of the ends of the bag. The joinder lines
42 and 44 are also preferably and most conveniently produced by
heat seals. Thus, it will be apparent from FIG. 1 that the
longitudinal access opening 30 is completely bounded by a
continuous bond or heat seal which is completely impervious to the
passage of bacteria and foreign matter; likewise, the interior of
the bag is completely isolated against the entry of bacteria and
foreign matter. The transverse heat seal 42 along the end of the
bag having the pull tab 36 may be spaced somewhat further from the
corresponding edge of the bag than the seal 44 so that a sufficient
pull tab area is exposed.
The adhesive coating 14 is applied to the outer surface of the
container along a longitudinally extending rectangular area
adjacent the lower edge of the closure membrane 12. The adhesive
coating 14 is covered by te protective strip 16 of wax-impregnated
paper or the like. The adhesive coating 14 may comprise any
pressure sensitive bonding material such as a latex type adhesive
or the like.
Indicia, which may be in the form of parallel broken lines 46 and
48 printed on the container 10, insure that the access opening 30
is in proper relationship with the adhesive coating 14 when the bag
is folded for sealing after the sample has been inserted. In the
example under consideration, the upper broken line 46 is positioned
parallel with and just below the longitudinal edge 20 and the lower
broken line 48 is placed parallel with and below the upper boundary
of the adhesive coating 14. It will be evident that the placement
of the indicia may vary according to the particular design but in
any event, when the bag is folded for sealing, the longitudinal
edge 20 should lie approximately along the longitudinal center line
of the adhesive coating 14.
The bag may have appropriate printed matter including blanks to be
filled in with the identification of the sample collected, and
other information. The closure membrane 12 may likewise include
printed matter and also have marginal indicia showing the points at
which the membrane may be most readily gripped for removal and the
preferred direction of removal.
The bags may be fabricated individually on suitable bag-making
apparatus such as the "Simplex" machine manufactured by FMC Corp.
of San Jose, Calif. In these machines, plastic sheet stock is fed
intermittently in successive steps, heat sealed across the ends and
cut into separate bags. Such standard bag-making machines require
certain modifications the nature of which are unimportant to the
disclosure of the present invention but which facilitate the
production of the bags of the present invention at an extremely
high rate with a great deal of reliability, accuracy and
repeatability.
Generally, the sheet stock forming the container 10 is fed from a
supply roll, folded over along the longitudinal fold lines 22 and
24 as explained and joined to the closure membrane 12 to form a
composite tube stock. The closure membrane 12 is fed concurrently
with the plastic sheet stock and is fed along the longitudinal
access opening 30 and into contact with the outer surface of the
container 10. The adhesive coating 14 may be simultaneously and
continuously applied along with the overlying protective strip 16.
The longitudinal joinder lines 32 and 34 are added by a heat seal
mechanism using longitudinal parallel spaced heater bars and
actuated intermittently in timed relation with the feeding of the
stock material. The transverse heat sealing operations and the
cutting operation may take place in conventional fashion using, for
example, either roller elements or heat seal bars to apply the
transverse heat seals and either a mechanical or thermal cutter to
separate the tube stock adjacent the transverse heat seals 42 and
44 thereby forming individual bags.
Following fabrication of the bag, the interior thereof is
sterilized by any of the well-known sterilizing vapor techniques
such as the ethylene oxide process. The interior of the bag will
remain in sterile condition over an extended period of time,
typically in excess of one year and may thus be stored until ready
for use.
In accordance with one practical example of a sample bag embodying
the invention, the bag has an overall length of 11 1/4 inches and
an overall width of 18 inches. The closure membrane 12 and
protective strip 16 are in abutment and have widths of 3 inches and
1 1/2 inches respectively. The width of the adhesive coating 14 is
also 1 1/2 inches. The upper edge of the closure membrane 12 lies
about 5/8 inch from the upper longitudinal fold line 22. The
portions 26 and 28 of the container have an overlap of 1 inch, the
longitudinal edge 20 lying about 1 3/4 inches from the fold line
22. The length of the pull tab 36 is about 1/2 inch and the broken
line indicia 46 and 48 are spaced 2 inches apart, the upper line 46
being positioned about 1/8 inch below the longitudinal edge 20.
Turning now specifically to FIGS. 3-7, the utilization of the
sample bag of the present invention will be described. First, the
closure membrane 12 is removed by grasping the tab 36 and pulling
the membrane toward the right, as viewed in FIG. 3. Next (FIGS. 3
and 4), the protective strip 16 is pulled away from the adhesive
coating 14. If there are two persons handling the sample collection
operation, as is usually the case, the sample loading technique
shown in FIG. 5 may be employed. One person holds the sample bag
with one hand along the upper margin thereof. By lightly contacting
the adhesive coating 14 with the thumb of the other hand, and
applying a gentle pull, the overlapping portions 26 and 28 of the
bag are separated to open the access opening 30. The other person
then drops the sample 50 into the bag which is then sealed (FIGS. 6
and 7) by folding the upper part of the bag along a longitudinal
line about mid-way between the indicia lines 46 and 48 to bring the
lines into registry and firmly pressing the portion of the bag
about the access opening into engagement with the adhesive coating
14. The access opening 30 is thus completely sealed by the adhesive
coating 14 against the entry of bacteria or other foreign matter.
Although it appears from FIG. 7 that bacteria may enter through an
opening below the edge 20, because of the thinness and flexibility
of the plastic sheet and the flowability of commercially available
pressure - sensitive adhesives which may be used for the adhesive
coating 14, said opening, below edge 20, does not exist in actual
practice. If only one person is available to both manipulate the
sample bag and insert the sample, a small area of the adhesive
coating 14 may be pressed lightly into engagement with a stationary
object. With one hand the rest of the bag is pulled away to open
the access opening 30 and the sample 50 is then inserted with the
other hand.
* * * * *