U.S. patent number 3,815,000 [Application Number 05/363,824] was granted by the patent office on 1974-06-04 for levitator.
This patent grant is currently assigned to Science Spectrum, Inc.. Invention is credited to Herman H Brooks, Chelcie B. Liu, David T. Phillips, Philip J. Wyatt.
United States Patent |
3,815,000 |
Phillips , et al. |
June 4, 1974 |
LEVITATOR
Abstract
A levitator for use with a light scattering photometer unit
including a spaced pair of plate electrodes to provide an electric
field for producing a first electrostatic force on a charged
particle located between the spaced plate electrodes and with the
levitator additionally including a pin electrode extending through
and insulated from one of the plate electrodes to provide an
electric field for producing a second electrostatic force and with
the combination of the first and second electrostatic forces
suspending the charged particle between the plate electrodes at a
location spaced from but adjacent to the pin electrode. An
automatic servo system includes an optical detector for detecting
the position of the charged particle to produce a control signal to
adjust the electric fields to maintain the charged particle in the
proper position.
Inventors: |
Phillips; David T. (Goleta,
CA), Brooks; Herman H (Goleta, CA), Wyatt; Philip J.
(Santa Barbara, CA), Liu; Chelcie B. (Santa Barbara,
CA) |
Assignee: |
Science Spectrum, Inc. (Santa
Barbara, CA)
|
Family
ID: |
26886993 |
Appl.
No.: |
05/363,824 |
Filed: |
May 25, 1973 |
Related U.S. Patent Documents
|
|
|
|
|
|
|
Application
Number |
Filing Date |
Patent Number |
Issue Date |
|
|
191373 |
Oct 21, 1971 |
3754830 |
|
|
|
Current U.S.
Class: |
361/233;
310/90.5; 356/343 |
Current CPC
Class: |
G01N
21/53 (20130101); G01N 15/0205 (20130101) |
Current International
Class: |
G01N
15/02 (20060101); G01N 21/53 (20060101); G01N
21/47 (20060101); G01n 021/00 (); B01d
059/44 () |
Field of
Search: |
;308/10 ;317/262E |
References Cited
[Referenced By]
U.S. Patent Documents
Other References
Physical Review, Vol. 32, pg. 349, first series, R. A. Millikan.
.
Physical Review, Vol. 4, pg. 440, second series, Fletcher..
|
Primary Examiner: Miller; J. D.
Assistant Examiner: Moose, Jr.; Harry E.
Attorney, Agent or Firm: Smyth, Roston & Pavitt
Parent Case Text
This is a division of application Ser. No. 191,373 filed Oct. 21,
1971, now U.S. Pat. No. 3,754,830.
Claims
We claim:
1. A levitator for suspending charged particles, including
a pair of parallel plate electrodes,
a third pin electrode extending through and insulated from one of
the pair of parallel plate electrodes and spaced from the other
electrode,
first means for supplying an electrical potential between the pair
of parallel plate electrodes to produce a first electric field in a
first direction,
second means for supplying an electrical potential between the pin
electrode and the pair of parallel plate electrodes to produce a
second electric field in a second direction opposite to the
direction of the first electric field to form a composite field
between the pair of plate electrodes to balance any charged
particles located between the pair of plate electrodes and to
produce a third radial electric field to pull any charged particles
located between the pair of plate electrodes toward a position
adjacent to the pin electrode.
2. The levitator of claim 1 wherein the first and second means
simultaneously supply electrical potentials to the electrodes.
3. The levitator of claim 1 additionally including means for
supplying individual charged particles between the pair of plate
electrodes.
4. The levitator of claim 1 additionally including means detecting
the position of a charged particle located between the electrodes
and for providing an error signal representing the difference
between the actual position of the charged particle and a desired
position for the charged particle and for adjusting the potential
applied to the electrodes to adjust the positions of the charged
particle.
5. A levitator for suspending microparticles in a fixed location to
be intercepted by a beam of light, including
an electrode structure including a pair of electrodes spaced from
each other and supplied with an electrical potential between the
pair of electrodes to produce a first electric field on a
microparticle located between the pair of electrodes and including
a third electrode extending through and insulated from one of the
first pair of electrodes and supplied with an electrical potential
between the third electrode and the pair of electrodes to produce a
second electric field to oppose the first electric field on the
microparticle located between the pair of electrodes to balance the
microparticle between the first pair of electrodes and to produce a
third electric field to pull the microparticle to a position
adjacent to the third electrode.
6. The levitator of claim 5 wherein the pair of electrodes and the
third electrode simultaneously receive electrical potentials.
7. The levitator of claim 5 additionally including means for
supplying individual microparticles between the pair of
electrodes.
8. The levitator of claim 5 additionally including means for
detecting the position of a microparticle located between the pair
of electrodes and for providing an error signal representing the
difference between the actual position of the microparticle and a
desired position for the microparticle and for adjusting the
potential applied to the electrodes to adjust the position of the
microparticle.
Description
The present invention is directed to a levitator for use with a
light scattering photometer unit. As one specific example, the
light scattering photometer unit may operate in the following
manner. Microparticles, whose light scattering properties are to be
investigated, are located in a beam of light produced by a light
source, preferably the light energy produced by the light source is
polarized and at a single frequency, such as the light energy
produced by various lasers. The microparticles may be of many
types, such as bacteria or latex spheres of about 1 micrometer in
diameter, but the levitator of the present invention could also be
used with particles of greater or lesser size. The light scattered
by the microparticle is intercepted by a detector mounted beneath a
periscope which moves in an arc around the particle. The signal
from the detector is amplified to drive a recorder to record a plot
of the scattered light intensity as a function of the angle of the
detector relative to the incident beam of light, this plot being a
differential light scattering pattern.
A clearer understanding of the operation of a light scattering
photometer may be had with reference to U.S. Pat. application Ser.
No. 777,837, filed on Nov. 21, 1968, in the name of Philip J.
Wyatt, and U.S. Pat. Ser. No. 34,243, filed on May 4, 1970, in the
names of Philip J. Wyatt, et al., and both assigned to the same
assignee as the instant application.
In the present invention, a stream of microparticles whose light
scattering properties are to be investigated is introduced into a
scattering chamber such as by nebulizing a liquid suspension or by
any other convenient means. Most microparticles upon being
nebulized from a liquid suspension or when collected from an
aerosol source naturally tend to have a small positive or negative
charge. An individual microparticle is isolated and positioned in
the center of a laser beam through the use of pneumatic and
electrical controls. Once the particle is suspended in the laser
beam, the particle may be automatically held in position by means
of an automatic servo mechanism so that the light scattering
properties of the particle may be investigated.
Generally, the particle is maintained in the proper position
through the use of a levitator which includes a pair of parallel
plate members serving as electrodes and with a third pin electrode
extending through but insulated from one of the parallel plate
electrodes. The combination of the three electrodes provides for
electric fields to maintain the particle suspended between the
parallel plates and in a position for interception with the light
beam.
In the prior art it is known to use a pair of parallel plate
electrodes to suspend a particle between the electrodes. For
example, Millikan, in the early part of this century, used parallel
plate electrodes to suspend small oil drops between the plates.
Generally, with the prior art apparatus, the plates were charged to
provide an electric field interacting with the charge of the
particles to counterbalance the force of gravity to maintain the
particle between the plates. The difficulty with this type of
apparatus is that the particle would not necessarily be centered
and would tend to move off from between the plates. In addition,
the electric field would have to be very accurately maintained and
parallel to the gravitational field which required that the plates
be very accurately aligned and perpendicular to the local
gravitational field or the particle would tend to move within the
space between the plates. These above difficulties made the use of
the Millikan-type apparatus impractical other than for
demonstrations.
In order to overcome some of the difficulties of the Millikan
apparatus, Fletcher, in 1914, added a third electrode which took
the form of a small plate located within and insulated from one of
the parallel plates, which third electrode was used to produce an
electric field to pull the particle in toward the center, and also
to counterbalance the gravitational forces on the particle. By
using this center electrode at selected times to produce an
electric field between the center electrode and the pair of
parallel plate electrodes, the particle could be returned to the
center position. The difficulty with the Fletcher apparatus is that
the size of the radial field produced by the small third electrode
and used for centering is limited by the fact that, in addition to
producing the radial field, the third electrode also produces a
vertical field. The vertical field is limited to a level to
counterbalance gravity which, in turn, limits the size of the
radial field. The Fletcher apparatus, therefore, provides for a
very weak radial field which is often not effective.
In order to overcome the shortcomings of both the Millikan and
Fletcher apparatus, the present invention uses a pair of spaced
parallel plate electrodes and with a pin electrode extending
through and insulated from one of the plate electrodes. In a
preferred embodiment of the invention the pin electrode extends
through the top electrode and wherein the two parallel plate
electrodes are charged to provide an electric field to produce a
force which is "down" to add to the gravitational force on the
particle. In order to compensate for this electric field produced
by the parallel plate electrodes, the center pin electrode is
charged relative to each of the plate electrodes to provide a field
to produce a force which is "up" to overcome both the effects of
gravity and the electric field pushing down thereby balancing the
particle between the plates. This "up" field is therefore
relatively large since it must overcome both the "down" field and
gravity.
In addition to the "up" field produced by the pin electrode, a
radial field is also produced, which is quite large since it is
produced by the same charge as the "up" field. This combination of
fields provides a strong radial field to maintain the particle in a
centered position while the pair of fields, one "up" and one
"down," is used to overcome the effects of gravity to maintain the
particle between the plates. Once the particle is in the center
position, a servo system with an optical detector may adjust the
magnitude of the fields to keep the particle suspended in the
proper position.
The above arrangement of two parallel plates forming a pair of
electrodes and with a third electrode extending through and
insulated from one of the parallel plates so as to provide for a
strong radial field while still balancing the particle may be
energized in a number of different ways. For example, the
electrodes may be energized alternately so that the "up" and "in"
field can be produced alternately with the "down" field to produce
the strong net radial field. The alternation must be rapid relative
to any rate of motion of the particle. In addition, both fields may
be produced simultaneously so as to produce the strong net radial
field while still providing the balancing of the particle. The
invention will be described with reference to an arrangement
wherein the fields are produced simultaneously, but it is to be
appreciated that the fields could be applied alternately, as
explained above.
A clearer understanding of the invention will be had with reference
to the following description and drawings, wherein:
FIG. 1a, 1b, and 1c illustrate in schematic form the operation of
the three-electrode levitator of the present invention;
FIG. 2 illustrates in detail a particular construction for a
scattering cell including a levitator for receiving and suspending
the microparticles;
FIG. 3 illustrates a general arrangement of the scattering cell 1
of FIG. 2 in combination with the various pneumatic controls for
introducing microparticles to the cell;
FIG. 4 illustrates a view of the control panel used to provide the
electrical and pneumatic control of the microparticles within the
scattering cell of FIG. 2;
FIG. 5 illustrates the arrangement of the optical detector and
other optics for use with scattering cell of FIG. 2; and
FIG. 6 illustrates a block diagram of a servo for controlling the
position of the microparticles within the scattering cell.
Referring first to FIGS. 1a, 1b, and 1c, a schematic representation
of the levitator of the present invention is shown. Specifically,
the levitator includes a pair of parallel plate electrodes 10 and
12 and a third pin electrode 14 extending through but insulated
from the electrode 10. As shown in FIG. 1c, the various electrodes
may have potentials applied thereto, the values of which, shown in
FIG. 1c, are representative only. For a given particle, the
electrode 10 may have a reference potential or be at ground, the
electrode 12 may have a potential on the order of +50 volts, and
the electrode 14 may have a potential on the order of +100 volts.
These positive potentials for the electrical energy applied to the
electrodes 12 and 14 are based on an assumption that the
microparticle to be suspended has a negative charge as shown by
charged particle 16 located in a suspended position. If the
particle had a positive charge, instead, then the corresponding
voltages on electrodes 10, 12 and 14 would be reversed.
Referring now to FIG. 1a, it can be seen that the difference in
potential between the electrodes 12 and 14 provides for strong
electric field producing a force which is "up" and "in" as shown by
arrows 18. The difference in potential between the electrodes 10
and 14 provides for strong electric field producing a force which
is radial and "in," as shown by the arrows 20. If the electrode 12
had no potential, a negatively charged particle would therefore be
drawn toward the center pin electrode 14.
Referring now to FIG. 1b, the difference in potential between the
electrodes 10 and 12 provides for weak electric field producing a
force which is "down," as shown by the arrows 22, and if no
potential were applied to electrode 14, a negatively charged
particle would be pulled downward toward the electrode 12. Since
the particle also is pulled downward by the force of gravity, the
electric field as shown by the arrows 22 is in a direction to
increase this downward movement.
FIG. 1c shows the combination of these electric fields.
Specifically, the strong radial electric field 20 is still present
to hold the particle 16 in a center position. A combined vertical
field 24 is a combination of the fields 18 and 22 shown in FIGS. 1a
and 1b and is weaker than the field shown by the arrows 20. This
weak vertical field is used to counterbalance the force of gravity
of the charged particle 16. It can be seen, therefore, that by the
use of this three-electrode structure, and specifically in the
particular manner in which this three-electrode structure is
energized, the gravitational forces acting on the charged particles
may be counterbalanced by a weak vertical field, while at the same
time a strong radial field is used to pull the charged particle
into a central position.
It is to be appreciated that the pin electrode 14 may extend
through the bottom electrode instead of the top electrode and with
the electric fields arranged to provide for the charged particle
properly positioned between the electrodes. Also, the plane of the
plate electrodes need not be perpendicular to the force of gravity
and the plate electrode may be disposed in any angular relationship
to the force of gravity and with the charged particle still held
suspended between the plate electrodes.
FIG. 2 is an exploded view of a scattering cell including a
levitator of the present invention to receive microparticles and to
provide for the detection of the differential light scattering
properties of these particles. Since air currents may exert a
substantial force on a suspended particle, preferably all parts of
the scattering cell are pneumatically sealed to one another, as by
O-rings, and the inlet and outlet also include means permitting
them to be selectively pneumatically sealed from exterior pressure
fluctuations. The scattering cell includes a cover member 100,
having an inlet connector 102 and a flush connector 104. The cover
fits over a settling chamber 106 which receives the microparticles.
The cover and settling chamber are sealed by an O-ring 107. The
base 10 of the settling chamber 106 is the first upper electrode 10
shown in FIG. 1. The pin electrode 14 extends through an insulating
plug 108 which is received in an opening 110 in the base 10.
An electrical connector 112 is mounted on the outside wall of the
settling chamber 106 to provide for the application of electrical
potential through the wire 114 to the pin electrode 14. The plate
electrode 10 and the settling chamber 106 are grounded through the
electrical connector 112. A bellows connector 116 extends through
the wall of the settling chamber 106. An opening 118 in the
insulator 108 allows microparticles to pass from the settling
chamber 106 into the light scattering area of the scattering cell
of FIG. 2.
The light scattering area is formed by a transparent cell member
120 which includes a masked section 122. Light energy, such as from
a laser beam, passes into the transparent cell 120 through an
entrance mask 124. A light trap 126 receives the light energy after
it passes through the transparent cell. A light exit 128 may be
provided through the masked portion 122 so that the interior of the
cell may be visually observed with a microscope in a manner to be
described in a later portion of this specification. This sealed
light exit is wedged at a slight angle to the axis of the
scattering cell in the preferred embodiment to avoid reflecting
light back into the cell in the horizontal plane in which scattered
light is viewed. The settling chamber 106 and the transparent cell
120 are sealed by an upper O-ring 130.
The light energy, when appropriately directed through the
transparent cell 120 from the entrance port 124, intersects any
microparticle located at the proper position in the transparent
cell and provides for differential light scattering in accordance
with the differential light scattering properties of the
microparticle. This differential light scattering may be detected
by viewing the differentially scattered light at different angular
positions through the transparent portion of the transparent cell
120.
A base member 132 supports the lower plate electrode 12. The base
member 132 and the transparent cell 120 are sealed using a lower
O-ring 134. An opening 136 extends through the lower plate
electrode and is connected to an exhaust connector 138 to provide
the exhausting of microparticles within the transparent cell 120.
Finally, an electrical connection for applying potential to the
lower plate electrode 12 is provided by electrical connector 140
which may be an opening to receive a plug, such as a banana
plug.
FIG. 3 illustrates a typical manner in which the scattering cell of
FIG. 2 may be interconnected with other elements to introduce
microparticles to the transparent cell. Specifically, a nebulizer
200 may be used to provide individual microparticles to the
scattering cell through a hose 202 connected to the inlet connector
102. The nebulizer 200 operates in a known manner using a supply of
a filtered gas, such as air to atomize portions of a liquid
suspension of microparticles and provide a stream of the gas
containing individual microparticles. An another example,
microparticles may be introduced by drawing an aerosol of
microparticles into a syringe, thus connecting the outlet of the
syringe to line 202 and selectively injecting the entrained
microparticles by collapsing the syringe.
When a nebulizer is employed, a supply of air is coupled through a
hose 204 and is connected to an air control valve 206. The air
control valve 206 has two outputs, one of which is through a hose
member 208 directly to the flush connector 104. An air control
switch 210 controls the application of air from the hose 204
through the hose 208 to flush out the scattering cell. A second
output from the air control valve 206 is through a hose 210 to a
nebulizer control valve 212. The output from the nebulizer control
valve is through a hose member 214 which is connected to the
nebulizer 200. A nebulizer button control member 216 is used to
control the application of air to the nebulizer 200. When the
button 216 is pushed, air is supplied to the nebulizer 200 to
provide for the introduction of microparticles contained in the
stream of air through the hose 202 to the inlet connector 102.
A bellows assembly 218 is used to supply a gentle supply of air
through a hose member 220 to the bellows connector 116. Finally, an
exhaust hose 222 is connected to the exhaust connector 138 to
provide for an exhaust of any microparticles within the scattering
cell. The interior of the scattering cell is pneumatically isolated
from any external air pressure changes and the flow through the
scattering cell is controlled by the bellows assembly 218 and the
nebulizer 200.
FIG. 4 illustrates the control panel of the levitator of the
present invention. As shown in FIG. 3, the control panel includes
an air control switch 210 and a nebulizer button switch 216. In
addition, the control panel of FIG. 4 includes a polarity control
knob 224, a voltage control knob 226, a balance control knob 228,
an automatic-manual-position knob 230, a bellows control knob 232
and a focus control knob 234. Also, an eyepiece 236 of a microscope
extends from the control panel.
The use of the various control knobs shown on the control panel of
FIG. 4 are as follows:
The air control switch 210 in its forward position provides for the
flushing of the scattering cell with a clean gas, such as filtered
air. In the rear position of the switch 210, the air control valve
206 shown in FIG. 3 is connected to have the air supplied to the
nebulizer control valve 212. The nebulizer control button 216, when
pushed, supplied air to the nebulizer to spray particles from the
nebulizer into the scattering cell. The polarity knob 224 controls
the electrode voltages to be either plus or minus so that particles
of either charge polarity can be levitated within the scattering
cell.
The voltage knob 226 sets the maximum voltage which may be applied
to the scattering cell electrodes. The balance knob 228 is set to
balance the input to the servo system for the desired position of
the particle. The position knob 230 may be in one of two modes,
depending upon whether the knob is pulled out or whether the knob
is pushed in. When the knob 230 is pulled out, the levitator is in
the manual mode. Rotating the knob 230 adjusts the voltages
provided to the electrodes so as to move the particle within the
scattering cell. When the knob is pushed in, the levitator is in
the automatic mode, and the levitator servo controls the electrode
voltages to maintain the particle in the proper position within the
scattering cell.
The bellows control knob 232 is used to flex a small bellows to
move air and particles slowly through the scattering cell. Finally,
the focus control knob 234 is used to focus the microscope so as to
visually observe the center of the scattering cell.
FIG. 5 illustrates the optical arrangement for visually observing
the center of the scattering cell and for providing for an optical
detection of the position of the particle within the scattering
cell. The light energy scattered by the particle near the center of
the scattering cell, as shown by arrows 300, leaves the scattering
cell through the exit 128 and is focused by a lens structure 302
onto a beam splitter 304. A portion of the light energy passes
through the beam splitter 304 to a diagonal mirror 306. A second
portion of the light energy is reflected by the beam splitter 304
to a viewing mirror 310 and may be viewed through a microscope 308.
When the eyepiece 236 of the microscope 308 is properly focused, a
visual observation may be had of the central region of the
scattering cell.
The light energy which impinges on the diagonal mirror 306 is
directed upward toward a second diagonal mirror 312. A portion of
the light energy is reflected by the second diagonal mirror 312 to
a first photomultiplier 314. In addition, a portion of the light
energy from the diagonal mirror 306 passes by the second diagonal
mirror 312 to impinge on a second photomultiplier 316. The
combination of thd two photomultipliers 314 and 316 with the second
diagonal mirror 312 may be used to provide an optical detection of
the vertical position of the particle within the scattering cell.
For example, if there are no particles in the scattering cell in
the path of the laser beam, then no light energy would be scattered
out of the exit 128 and no light energy would therefore pass to the
diagonal mirror 306. If there is a particle in the path of the
light beam within the scattering cell, then the position of this
particle determines the intensity distribution of the scattered
light passing through the exit which in turn determines the amount
of light energy which is received by each of the two
photomultipliers 314 and 316. In this way, the vertical position of
the particle determines the relative output of the photomultipliers
314 and 316.
FIG. 6 illustrates the operation of the automatic servoing of the
levitator so as to maintain the microparticle in the proper
position within the scattering cell. In FIG. 6 the three-electrode
levitator of the present invention, including the parallel plate
electrodes 10 and 12 and the center pin electrode 14 is shown with
the plate electrode 10 grounded and with voltages applied to the
electrodes 12 and 14. A laser beam supplies light energy to the
scattering cell and a scattered portion of the light energy is
directed toward the mirror 312. The scattered light strikes the
edge of the mirror 312 which divides this scattered light between
the two photomultiplier tubes 314 and 316. The intensity of the
light received by the two photomultiplier tubes 314 and 316 is
equal when the particle is in the proper position within the
scattering cell.
The output from the photomultipliers 314 and 316 are applied to a
pair of matched characteristic logarithmic input amplifiers 350 and
352, which logarithmic amplifiers convert the photomultiplier
output current to a voltage proportional to the logarithm of the
current from the photomultiplier. The use of logarithmic amplifiers
maintains constant servo gain and provides servo stability for a
wide range of particles. The balance control 228 is adjusted to
provide equal gain for equal signals into the input amplifiers 350
and 352. The outputs from the amplifiers 350 and 352 are applied to
a difference circuit 354 and the difference between the two
voltages is amplified by isolation amplifier 356 and applied to a
sample and hold circuit 358. The output of the sample and hold
circuit 358 is controlled by a one-shot multivibrator 360 which
controls a switch 362. Since a pulsed laser is used in a preferred
embodiment, the output signals from the input amplifiers 350 and
352 are used to control the multivibrator 360. If a continuous wave
laser is used, the sample and hold circuit may be ommitted, or
replaced by an averaging circuit.
The position control 230(a) which is the resistive control portion
of the positioning control switch 230 shown in FIG. 4 is used to
control an input signal to an isolation amplifier 364. In addition,
the position control switch 230 may be either in a manual or an
automatic mode as controlled by the switch portion 230(b) of the
position control. The manual mode is when the switch 230(b) is
closed, which controls the multivibrator 360 to maintain the switch
362 in an open position. At this time only the position control
230(a) effects the voltages applied to the electrodes of the
levitator. When the switch 230(b) is in the open position, the
servoing is automatic. At this time input signals are provided from
the differencing circuit 354 to the sample and hold circuit 358 so
that the output of the sample and hold circuit is an error signal
in accordance with the position of the particle.
As indicated above, the output from the sample and hold circuit 358
is applied to the isolation amplifier 364 and then to a pair of d-c
to d-c converters 366 and 368. The maximum output from the d-c to
d-c converters 366 and 368 is adjusted by the voltage control 226,
but below that maximum value the output from the d-c to d-c
converters are control signals which are applied through the
polarity switch 224 to the electrodes 12 and 14 to maintain the
particle in the proper position within the scattering cell.
The operation of the levitator in isolating and positioning an
individual particle within the laser beam with the structure shown
in FIGS. 2-5 is as follows:
First, the nebulizer 200 is filled with a suspension of the
microparticles to be studied and the hoses are connected to the
scattering cell in the manner shown in FIG. 3. The various
electrical power requirements are coupled to the instrument so that
the laser (or other light source) is energized and the electrodes
in the levitator are also energized. Initially the polarity control
224 may be positioned to the plus position. The voltage control 226
is adjusted to zero volts which is normally in the full
counterclockwise position. The position control 230 is in the
manual mode and turned counterclockwise which normally means that
the particle is pulled downward.
In order to clear the scattering cell, the air toggle switch 210 is
switched forward to flush the scattering cell with clean air. This
may be visually observed by watching through the eyepiece 236 of
the microscope 308 until no particles can be seen passing through
the laser beam. When this has occurred, toggle switch 210 is
returned to the rear position to connect up the nebulizer 200. The
nebulizer button 216 may then be pushed briefly a few times until
several particles are observed passing through the laser beam with
each pulse of the nebulizer button 216. Now the bellows knob 232
may be moved back and forth to move particles slowly through the
beam. The larger particles may be easily seen and for smaller
particles the defraction images of the particles appear as sharply
defined concentric rings will be visible through the microscope.
The focus knob 234 may be adjusted for maximum image sharpness.
When there is a bright image in the field of view of the
microscope, representing a particle, the voltage control 226 may be
turned up until the image can be observed moving in response to the
voltage control 226. The motion of the particle should be in the
downward direction. If the motion is not downward, this means that
the polarity of the particle is reversed and the polarity control
224 should be switched to the minus position. The position knob 230
may be moved to arrest the downward motion of the particle and
bring the particle back toward the center of the field of view of
the microscope. Actually, both the voltage and position controls
may be simultaneously manipulated so as to stop the particle's
motion before it leaves the region within which it can be seen.
If the particle is lost, then the voltage and position controls may
be returned to zero and the nebulizer button pushed again to bring
new particles in the field of view. Since the particles may have
different polarities, it is important to remember that the polarity
control may have to be reversed in order to provide the downward
motion. As an alternative to the above, it may be more convenient
to begin with the polarity control 224 at the zero position and
with the voltage control 226 set high enough to produce relatively
fast motion. The positioning of the particle may then be
accomplished by simultaneously manipulating the polarity and
position controls instead of the voltage and position controls.
When a particle has been positioned in the center of the field of
view and remains nearly stationary, then the position control 230
is pushed in to place the levitator in the automatic mode. This
engages the automatic servo control shown in FIG. 6 so as to hold
the particle fixed within the light beam. The voltage control 226
may be turned completely up so as to provide a maximum automatic
servoing of the position of the particle within the levitator.
The present invention therefore provides a simple structure for
positioning a microparticle within a beam of light, such as light
from a laser, and maintaining that particle automatically in this
position. The levitator includes a pair of parallel plate
electrodes and a third electrode extending through and insulated
from one of the plate electrodes. In a preferred embodiment of the
invention, the pair of parallel plate electrodes provide an
electric field to pull the particle down to aid the force of
gravity and with the center electrode providing an electric field
to pull the particle up and counterbalance the downward forces and
also to pull the particle toward the center. This structure allows
for a relatively strong electric field to be used to pull the
particle into the central position. The maintaining of the position
of the particle may be automatically controlled using an optical
detector in combination with a servo system.
The invention has been described with reference to a particular
embodiment but the invention is only to be limited by the appended
claims.
* * * * *