U.S. patent number 3,782,387 [Application Number 05/230,336] was granted by the patent office on 1974-01-01 for apparatus and methods for obtaining and making skin grafts.
Invention is credited to Rafael Falabella.
United States Patent |
3,782,387 |
Falabella |
January 1, 1974 |
APPARATUS AND METHODS FOR OBTAINING AND MAKING SKIN GRAFTS
Abstract
An apparatus and method are provided for obtaining skin grafts
and making skin grafts by subjecting a donor site to vacuum to
produce blistering of the epidermal layer and removing and
transferring the blister to the recipient site.
Inventors: |
Falabella; Rafael (Cali,
CO) |
Family
ID: |
22864831 |
Appl.
No.: |
05/230,336 |
Filed: |
February 29, 1972 |
Current U.S.
Class: |
128/898;
604/315 |
Current CPC
Class: |
A61B
17/322 (20130101); A61B 2017/306 (20130101); A61B
2017/00544 (20130101) |
Current International
Class: |
A61B
17/322 (20060101); A61B 17/30 (20060101); A61B
17/00 (20060101); A61b 017/32 () |
Field of
Search: |
;128/276-278,299,300,302,305,305.5 |
References Cited
[Referenced By]
U.S. Patent Documents
Other References
"The Lancet," Vol. I/64, No. 7348; June 27, 1964, pp.
1444-1445..
|
Primary Examiner: Rosenbaum; Charles F.
Attorney, Agent or Firm: Buell, Blenko & Ziesenheim
Claims
I claim:
1. An apparatus for obtaining epidermic grafts comprising a vacuum
source, an hermetic vessel connected to said vacuum source and
placed under vacuum thereby, a plurality of hemispherical cup
shaped suction capsules connected to said hermetic vessel and
adapted to be placed on an epidermal layer to be collected and
transverse flange means surrounding the edge of said cup shaped
capsule engaging and forming a seal with said epidermal layer to
prevent relative movement of the epidermal and flange.
2. An apparatus for obtaining epidermic grafts as claimed in claim
1 having a manifold connected to said hermetic vessel and a
plurality of said hemispherical cup shaped suction capsules
connected to said manifold whereby said cups are under uniform
vacuum.
3. An apparatus for obtaining epidermic grafts as claimed in claim
1 having vacuum measuring means connected to said hermetic
vessel.
4. An apparatus as claimed in claim 2 wherein the edges of said
plurality of suction capsules are connected to one another by a
flexible sheet having a sealing projection around its
periphery.
5. A method of epidermal grafting comprising the steps of:
a. forming a blister of the epidermal layer of a donor skin
site;
b. removing said blister from the donor site by cutting around its
periphery;
c. transferring the blister from the donor site to the recipient
site with the dermal face exposed;
d. attaching the dermal face of said blister to the recipient site;
and
e. smoothing said blister onto the contour of the recipient
site.
6. The method as claimed in claim 5 wherein the blister is
transferred by placing the external surface thereof onto a smooth
plate and applied by pressing said plate and blister with the
dermal face onto the recipient site and removing said plate.
7. The method as claimed in claim 5 wherein the blister is formed
by subjecting the donor site to a vacuum of about 200 mm. of
mercury.
Description
This invention relates to apparatus and methods for obtaining and
making skin grafts and particularly to an apparatus for obtaining
from a donor area skin grafts by suction and to a method of
obtaining skin grafts from a donor area and implanting them to a
receptor area.
The problem of obtaining skin grafts and applying them is an old
and well recognized one. Normally skin grafts are obtained by
incising a portion of donor skin with the patient under local or
general anaesthesia and moving the same to the receptor area. Such
grafts included a substantial thickness of both epidermal and
dermal tissue. They are painful, they frequently scar or at least
seriously discolor the donor area and they include an anaesthetic
risk in many patients.
There are many situations where achromic lesions and granulating
areas of the skin which could be corrected are simply ignored
because of the fact that skin grafting techniques presently
available are too complex, expensive and painful to warrant their
correction. This is particularly true in those areas of the world
with large numbers of dark-skinned individuals where achromic
lesions are particularly noticeable. Heretofore therapy has been
difficult and discouraging and no means has been available for
obtaining and applying pigment bearing grafts to cover achromic
lesions in a simple, inexpensive and relatively painless way.
Typical of such problems are achromic nevi, achromic areas in
healed ulcers of thermal burns.
The present invention provides a means and method for obtaining
skin grafts and applying them without the problems attendant with
ordinary skin grafting techniques. By the present invention it is
possible to obtain epidermal grafts without damaging the dermal
tissue, thereby avoiding scarring and disfigurement of the donor
area, without anaesthetic risk and with little pain to the
patient.
It is accordingly an object of the present invention to provide an
apparatus which makes possible the obtaining of epidermal grafts
with minimum discomfort and without anaesthetic risk.
It is another object of this invention to provide an apparatus for
obtaining epidermal grafts by suction blistering.
A further object of the invention consists in providing a method
for obtaining skin grafts and transferring the same to a receptor
area.
The apparatus of this invention makes it possible to obtain
practically a complete separation of the epidermis including the
basic layer, in a non-traumatic form, by means of negative
pressure. This is accomplished by applying an inverted cup-shaped
vessel over the epidermal area and leaving it in position, under
suction or negative pressure, for a more or less prolonged period
of time to produce a blister or bulla, which consists exclusively
of epidermis without producing any traumatism in the dermis.
Blistering techniques have heretofore been used for treating
various skin problems. For example it has been known for many years
that blisters could be produced by chemical means. More recently
suction techniques have been used for blister production. However
such techniques have not been designed for production of skin
grafts and the apparatus used in such early blister techniques
caused excessive traumatization of areas surrounding the blisters
and utilized excessive sealing areas so that the production of
blister areas was inefficient and not completely satisfactory for
most effective production of skin grafts.
Essentially, the apparatus of the invention is composed of a means
producing a vacuum greater than 200 mm. of H.sub.g connected to a
central vacuum unit from which goes a flexible tube which extends
the central vacuum unit hydraulically with a central suction unit
which consists of a hollow cylinder of plastic material having a
sealed end while at the other end it is connected to the central
vacuum unit and carries on its lateral surface a plurality of
plastic tubes each one of which is connected at its end to a cup or
capsule, preferably round provided with an edge of flange, by means
of which a greater hermetic seal is obtained and it is avoided that
the skin slides inside the cup during the suction.
So in the event that an appreciable quantity of tissue is necessary
it is possible to join the cups or capsules in one unit in which
their edges are joined on a plate of flexible material provided
with a perimetrical edge which has the same function as the flange
of the aforementioned cup or capsule. Through the condition of
flexibility of the plate, it is obtained that the plate adapts to
the form of the area of the skin which is the donor site so that
the skin can adhere readily when the suction is applied.
In the preferred practice of the method of this invention, both the
donor and receptor sites are cleansed, the donor area is blistered,
the blisters are cut around their periphery and transferred to a
smooth transfer surface with the external surface in contact with
the transfer surface and the dermal face exposed, the blister is
transferred to the receptor area on the transfer surface, an edge
of the blister is held against the receptor site while the transfer
surface is removed, leaving the blister on the receptor area and
the blister or graft is smoothed over the receptor area. The
transfer surface is preferably a glass microscope slide .
So that the invention may be understood easily and can be readily
put in practice it has been represented in its preferred working
form in the accompanying drawings in which:
FIG. 1 represents the apparatus diagramatically annd in one of its
preferred working forms;
FIG. 2 is a longitudinal cross-section view of the central suction
unit or manifold;
FIG. 3 is a lateral elevational view of one of the preferred
alternate forms of achieving the suction cups or capsules;
FIG. 4 is a front view of FIG. 3; and
FIG. 5 is a plan view of the form shown in FIG. 3.
A vacuum producing apparatus is shown in all the figures with the
same reference numerals applied to corresponding parts of the
invention. It is illustrated as a vacuum pump actuated by an
electric motor. The vacuum pump is indicated by reference 1, its
suction end is connected by a flexible hose 2 with a central vacuum
unit 3 which preferably has the form of a flask or bottle of more
or less a liter capacity and is provided with a rubber stopper 4
through which the connecting tube penetrates with the hose 2.
Through this rubber stopper 4 passes a tube 6 which by means of a
flexible hose 7 communicates with a vacuum manometer 8, while by
means of a tube 9 and hose 10 the inside of 3 is connected with a
central suction manifold unit or manifold 11 represented in detail
in FIG. 2 and composed of a walled chamber 12, base end 13 and
opposite end 14 provided with the spout 15 connecting with the hose
10.
The lateral walls 12 are provided with spouts 16 on each one of
which are attached corresponding flexible hoses 17 which through
suitable connections are joined to suction cups or capsules 18.
Preferably, these suction capsules or cups are composed of a hollow
hemi-spherical body whose edge is provided with a flange 19 whose
function is to obtain a better vacuum seal and prevent the skin
from sliding inside the capsule during suction.
In another of the preferred working forms of the invention, the
suction capsules or cups 18 are supported over a flexible plate 20
provided with a perimetrical edge 21 which constitutes a flange or
projection anchoring the plate 20 to the skin.
The inside of each of the suction capsules or cups 18 communicates,
by means of small short flexible hoses 23, with a manifold 22 to
which is connected the flexible hose 10 actuating the manifold 22
as central suction unit.
The suction capsules or cups 18 are preferably constructed of
transparent or semi-transparent plastic material so that their
interior can be seen.
The process for using the device of the invention consists in first
cleaning the donor area with soap and later alcohol; tincture of
benzoin is applied to insure better adherence of the suction
capsules to the skin. Then one by one the capsules are placed, each
of which has a pressure clamp or pincer on the connecting hose
which is released on being applied to the skin; the negative
pressure is first set at 200 mm. H.sub.g and is not changed with
application of the capsules. The donor area should preferably be
the inside face of the thigh, although other areas can be used.
Approximately 3 hours later the blister or bullas have appeared as
the final product of coalescence of small vesicles or blisters
which appear gradually during the suction period. They can be
observed through semi-transparent plastic of the capsules. The
pressure is then brought to zero and the capsules are removed from
the skin.
At this moment the blisters can be considered ready to be grafted.
The blisters are cut off with iris type scissors for dermatological
use at the level of their implantation. The epidermal sheet thus
obtained is manipulated with a hypodermic needle to place it on a
microscopic glass slide with the external face in direct contact
with the glass and the internal or dermic face to be applied
directly on the surface and exposed ready to be grafted. The glass
slide is moved immediately with adherence of the epidermic sheet so
that it is put in contact with the receiving area; an edge of the
epidermic fragment is held with a hypodermic needle and the glass
plate is lifted slowly until it is completely removed. Then the
graft is positioned finally with the two hypodermic needles and is
located according to the size and shape of the area to be
grafted.
In the foregoing specification I have set out certain preferred
embodiments and practices of my invention, however it will be
understood that this invention may be otherwise embodied within the
scope of the following claims.
* * * * *