U.S. patent number 3,770,027 [Application Number 05/216,050] was granted by the patent office on 1973-11-06 for manifold liquid distributor.
Invention is credited to Jean Guigan.
United States Patent |
3,770,027 |
Guigan |
November 6, 1973 |
MANIFOLD LIQUID DISTRIBUTOR
Abstract
The present invention provides apparatus for removing a measured
quantity of liquid by a series of capillary tubes with the
subsequent introduction of the liquid into respective work tubes
for the performance of, for example, chemical reactions. The liquid
is introduced into the capillary tubes by a piston action and then
subsequently forced passed a restrictive orifice under the
influence of a centrifugal action. Various forms of creating the
piston action are provided.
Inventors: |
Guigan; Jean (Paris,
FR) |
Family
ID: |
26216141 |
Appl.
No.: |
05/216,050 |
Filed: |
January 7, 1972 |
Foreign Application Priority Data
|
|
|
|
|
Jan 7, 1971 [FR] |
|
|
7100378 |
Feb 24, 1971 [FR] |
|
|
7106311 |
|
Current U.S.
Class: |
141/34; 141/245;
422/72; 141/236; 141/329; 422/500; 422/504 |
Current CPC
Class: |
G01N
31/22 (20130101); G01N 1/18 (20130101); G01N
1/10 (20130101); G01N 2001/1454 (20130101) |
Current International
Class: |
G01N
31/22 (20060101); G01N 1/18 (20060101); G01N
1/14 (20060101); G01N 1/00 (20060101); B65b
037/12 (); B01l 011/00 () |
Field of
Search: |
;141/31,34,54,57,58,59,99,100,234,236,283,285,286,297,312,325,329,330,331,332
;222/319 ;233/26 ;23/259 |
References Cited
[Referenced By]
U.S. Patent Documents
Primary Examiner: Petrakes; John
Assistant Examiner: Schmidt; Frederick R.
Claims
What is claimed is:
1. An apparatus for distributing liquid in discrete measured
quantities comprising:
a collection tube for holding the liquid to be distributed;
a plurality of capillary conduits having a size relative to the
type of liquid to be distributed to permit a capillary action
between the liquid and the conduits connected to the collection
tube;
throttling means in each of the conduits;
means for forcing and passing the liquid from the collection tube
into the conduits at least as far as the throttling means;
means for venting the collection tube thereby permitting the liquid
to be retained in the conduits to the extent of said throttling
means;
work tubes connected to each conduit for receiving its respective
retained quantity of liquid, and
means for forcing the liquid retained in the conduits into the
working tubes.
2. An apparatus as in claim 1 wherein: the means for forcing the
liquid from the collection tube comprising means for moving the
collection tube relative to the conduits to produce a piston action
on the liquid in the collection tube thereby forcing the liquid
into the conduits.
3. An apparatus as in claim 1 wherein the means for forcing the
liquid into the work tubes includes means for rotation of the
conduits to produce a centrifugal action.
4. An apparatus as in claim 1 wherein the means for forcing the
liquid from the collection tube includes a plunger conduit
connected to each of the conduits that receive a measured quantity
of liquid and wherein, at least a portion of the outside diameter
of the plunger conduit corresponds approximately to at least a
portion of the inside diameter of the collection tube.
5. An apparatus as in claim 4 wherein guide flanges are attached to
the plunger conduit.
6. An apparatus as in claim 4 wherein the plunger conduit and the
collection tube are respectively threaded to permit a screw action
between each other.
7. An apparatus as claimed in claim 4 wherein: the collection tube
has a central narrow position of the same inside diameter as the
outside diameter of the plunger conduit with the upper and lower
portions of the collection tube having larger diameters.
8. An apparatus as in claim 4 wherein the conduits extend to the
bottom of the plunger conduit, the plunger conduit having a vent
conduit sealed with a diaphragm and the collection tube having a
piercing stud located on its inside surface for piercing the
diaphragm.
9. An apparatus as in claim 4 wherein: the radially outer ends of
said conduits terminate in a sharp puncturing tip and the work
tubes having a sealing diaphragm across their surface designed to
be punctured by the conduit tips.
10. An apparatus as in claim 9 where the throttling means includes
a restricted orifice.
11. An apparatus as in claim 1 wherein an overflow tube is
connected to the conduits.
12. An apparatus as in claim 1 wherein the conduits are conically
arranged about the collection tube and the work tubes are arranged
approximately perpendicular to the plane of the cone.
13. An apparatus as in claim 1 wherein: the conduits terminate at
their radially outer ends, in a sharp puncturing tip and the work
tubes have a sealing diaphragm acros their surface designated to be
punctured by the conduit tips.
14. An apparatus as in claim 13 wherein: the means for venting
includes a groove adjacent the puncturing tip of each conduit.
15. An apparatus as in claim 1 wherein the conduits are bent
approximately 90.degree. before connection with the work tubes but
after the throttling means.
16. An apparatus as claimed in claim 1 further comprising a ring
portion for holding the work tubes at circumferentially spaced
positions.
17. An apparatus as in claim 1 wherein the conduits are arranged in
a conical plane and spiral outward fro the collection tube.
18. An apparatus as in claim wherein: the work tubes are divided
into two liquid retaining portions by a diaphragm, the top portion
adapted to hold one reactant while the bottom is adapted to hold
another reactant.
19. An apparatus as in claim 1 wherein; the conduits terminate at
their radial outer ends in a sharp tip, the work tubes have an
outer and inner sealing diaphragm spaced axially from each other
and a spacer is provided between said conduits and said tubes for
limiting the respective tip of each conduit to pierce only the
outer diaphragm when the outer ends of the tubes are connected to
the conduits.
20. An apparatus as in claim 19 wherein the conduits have an
adjacent bearing shoulder for receiving the spacer.
Description
BACKGROUND OF THE INVENTION
Field of the Invention
The present invention relates to a manifold distributor of a
measured liquid substance from a first container in which it is
stored to a plurality of secondary containers.
SUMMARY OF THE INVENTION
The aim of the present invention is to provide a distributor of
simple structure, which is low in cost and provides rapid and
reliable operation.
Another aim of the invention is the simultaneous distribution of
measured amounts of liquid into each of the secondary containers,
these amounts being preferably small and accurately measured.
The present invention has as its object a manifold distributor of a
liquid in measured amounts comprising a first tube for collecting
the liquid to be distributed and a plurality of secondary tubes
known as work tubes and intended for receiving the measured amounts
of the liquid, characterized in that it also comprises a plurality
of capillary conduits provided with throttling means, a piston
action cooperating with the collection tube to ensure the filling
of the conduits from the collection tube via a first end of the
former and at least as far as the throttling means, venting means
of the first end of the conduits, and centrifugal means cooperating
with the work tubes to ensure the transfer into the latter of the
liquid contained in the conduit.
BRIEF DESCRIPTION OF THE DRAWINGS
Other features of the manifold distributor according to the
invention will be given in the following description relating to
the attached drawings in which:
FIG. 1 is a sectional view along an axial plane of the device
according to the invention;
FIG. 2 shows a plan view of the unit for collecting the measured
amounts of the liquid;
FIG. 3 is a diagrammatic plan view of an embodiment of the
retention member;
FIGS. 4 and 5 show two embodiments of one of the members of FIG.
1;
FIG. 6 is a sectional view along an axial plane of an embodiment
with respect to FIG. 1;
FIG. 7 is an enlarged partial sectional view of a work tube and the
conduit associated with it according to an embodiment of the
invention;
FIGS. 8, 9 and 10 are sectional views of three respective positions
of the work tube and the conduit associated with it as shown in
FIG. 7.
DESCRIPTION OF THE PREFERRED EMBODIMENTS
As shown in the Figures, the manifold distributor comprises
essentially three parts intended to work together to ensure a
simultaneous distribution of measured amounts of a same liquid.
The manifold distributor comprises a storage tube 1 of the liquid,
known hereafter as the tube for collecting the liquid of which
measured amounts are to be distributed, a member 20 for the
retention of measured amounts of the liquid, the member ensuring
the drawing off of the liquid and its distribution in measured
amounts, and a plurality of storage tubes 30 for the measured
amounts of the liquid, the tubes being known hereinafter as work
tubes.
In FIG. 1, the collection tube 1 has a constant cross-section. The
tube 1 is open at its upper end. The member 20 consists of a hollow
tube 2 of slight section in relation to that of the collection tube
1, provided at a first open end intended to penetrate into the
collection tube 1, with a collar 3 of the same external diameter as
the internal diameter of the collection tube 1. Blades 4 disposed
along the length of tube 2 are intended to ensure the guiding of
the latter within the collection tube 1.
The second end of tube 2 opens into a central chamber 5 of
spherical shape in FIG 1, the outer wall of which is provided, over
a section along a plane perpendicular to the axis of the tube 2,
with a plurality of regularly disposed orifices 6 into which issue
a plurality of conduits 9 having a very small section. A tube 7
disposed in the extended part of tube 2 has a first end which also
debouches into the central chamber 5. The second end of tube 7
widens out into 8.
The conduits 9 ending in the orifices 6 of the central chamber 5
are disposed radially with respect to chamber 5. These conduits 9
are constituted by tubes, for example, capillary tubes. Each
comprises a throttling means 10, the volume between the end opening
into one of the orifices 6 and the section of the lowest diameter
of the throttling means being calibrated with precision.
The portions between the second ends of the conduit 9 and the
throttling means are bent outwards, the vertices of the bends being
at a similar or slightly higher level than that of the
funnel-shaped part 8 of the tube 7. The second ends of the conduits
9 are regularly distributed on a circumference having as an axis
the axis of the tube 2 or 7. These ends are intended to issue
respectively into a plurality of work tubes 30 joined one to the
other by means of an annular bearer element 31 on which they are
regularly spaced, the distance between the openings of two
successive work tubes 30 being equal to that between the second
ends of two successive conduits 9.
Each of the work tubes 30 is closed initially by a piece 17, the
central part of which forms an easily pierceable disphragm 12.
Above the disphragm 12, the piece 17 limits a recess 18 ensuring
the guiding into position of a piece 13 in which is sunk the second
end of the corresponding conduit 9 which is intended to open into
the relevant work tube 30. This piece 13 has a section end which is
substantially equal to that of the recess 18, the end terminating
in a tapered point turned towards the diaphragm 12 to be
pierced.
A circular band 14 of cardboard or the like may form a cross-piece
between the piece 13 and each corresponding diaphragm 12 and may
thus isolate the conical end of the piece 13 from the diaphragm 12
before utilization of the device. To use the device the band is
removed by using a circular tearing movement. An outwardly
projecting portion of the band allows it to be gripped for this
purpose.
Preferably, as illustrated in FIG. 1, a groove 15 made in the
conical end of the piece 13, supplemented by a groove 16 made in
the part of the piece 17 limiting the recess 18, ensures venting of
the conduit 9 when the diaphragm 12 has not been pierced, and
venting of the corresponding work tube 30 when the diaphragm 12 has
been pierced.
It will be noted in the example of the embodiment in FIG. 1, that
the conduits 9 are inclined with respect to the axis of the tube 2.
The work tubes 30 have, with respect to an axis passing through the
vertex of the bend and parallel to the axis of the tube 2, an
inclination which is substantially symmetrical with that of the
conduit 9 with which they are respectively associated.
Individual embodiments of the device will now be described.
The liquid to be distributed into the tubes 30 is collected in tube
1. The device described above is held horizontally; the tube 1 is
positioned onto the central tube 2 of the device so as to establish
a connection between the collection tube 1 and the central chamber
5. By applying pressure in the direction indicated by the arrow 11
on the base of the tube 1, the collar 3 adopts the role of a piston
and the liquid rises in tube 1 towards chamber 5.
In proportion to the distance the hollow tube 2 penetrates the
collection tube 1 and in dependence on the capacity of the
capillary tubes 9 the liquid rises in the conduit 9; either by
capillary attraction -- as soon as a sufficient amount of the
liquid reaches the chamber 5, the liquid rises as far as the level
of the throttling means 10 solely by virtue of the capillary force;
or by the action of the pressure maintained by continuing to push
the collection tube 1 in the direction of the arrow 11. In this
case, the level of the liquid is raised to the level of the
throttling means 10 in the conduit 9 and even beyond this point. At
the same time, the liquid rises to the same level in the tube 7 and
reaches the widened part 8 intended to control the maximum level
attained in the conduits 9 so that the liquid will in no event rise
above the level of the bend of each conduit 9 and flow over the
ends of the conduit 9.
In the two above cases, by subsequently drawing the collection tube
1 downwards, i.e., in the opposite direction to that of the arrow
11, or by pulling the unit 20 upwards the level of the liquid in
the conduits 9 and/or the tube 7, the chamber 5 and the tube 2 will
descend. Under the influence of the capillar forces at the level of
the throttling means 10, the level of the liquid in the conduit 9
will only descend to the level of these throttling means. In the
upper central tube 7, in the chamber 5 and in the lower central
tube 2, the level of the liquid descends until all the liquid
contained in these elements has flowed into the collection tube
1.
In this way, at the end of this operation, each conduit 9 contains
a measured amount of the liquid between its lower end and the
throttling means.
The device is then rotated about its symmetry axis (the axes of the
tubes 2 and 7) either by hand or with the aid of a small
centrifuge, which is not shown, and on which is mounted the end of
the central tube 2 removed from the collection tube 1.
The ends of the conduit 9 are then engaged respectively in the work
tubes 30, each piece 13 piercing the corresponding diaphragm
12.
Under the influence of centrifugal force, the amounts of liquid
contained in the conduit 9 pass from these conduits into the
corresponding work tubes 30 where they are mixed if necessary with
the solvent, a reagent or a known quantity of any other liquid or
solid substance placed in the work tubes.
Each work tube thus receives simultaneously an accurately measured
amount of liquid. Preferably, the measured amounts of liquid will
be identical from one tube to another.
To facilitate comprehension of the operation of the device, on the
lefthand side of FIG. 1 is shown the end of one of the conduits 9
discharging into the inside of the corresponding work tube 30, the
diaphragm 12 having been pierced, the grooves 15 and 16 thus
bringing the inside of the tube into contact with the outside of
the conduit. The end of one of the conduits 9 shown on the
righthand side of FIG. 1 has not pierced the corresponding tube 30
and is thus not in contact with the inside of this work tube.
In the form of embodiment shown in FIG. 1, collection tube 1 is of
a known conventional type. The member 20 for the drawing off and
distribution of measured amounts of liquid may be obtained, for
example, by superposing two half shells, a lower shell 21 an an
upper shell 22 having on their opposing faces grooves which
complement each other to form the interior parts of the conduit 9
and the central chamber 5 when the two half shells 21 and 22 are
assembled in a sealed manner at least along the edges of these
grooves. The lower half shell 21 comprises the lower central tube 2
fitted with the collar 3 and the blades 4 while the upper half
shell 22 comprises the upper central tube 7, the widened upper part
8 of the central tube 7 preferably being a part connected to the
tube 7.
The work tubes 30 which are integrally linked to one another by an
annular bearer element 31 and which are intended to receive
measured amounts of the liquid may be formed, as shown in FIG. 2,
by a series of regularly spaced cavities in a crown forming the
bearer element 31. The crown 31 is an inclined generatrix. The
cavities defined in the crown 31 are inclined with respect to the
axis of the crown 31, the centers of the openings of the tubes 30
being regularly distributed on a circle having a lower diameter
than that on which the central part of their respective bases are
regularly distributed. The openings of the cavities or work tubes
30 are sealed by a flat annular piece 19 having on one face, as
shown in FIG. 1, portions in relief, the dimension of each of which
are substantially equal to those of the openings of the work tubes,
such that the latter are sealed by the portions. This flat piece 19
has on its other face, hollowed portions to the right of the
central part of the portions in relief. These hollow portions form
the aforementioned recesses 18 which ensure the centering of the
second ends of the conduits 9 on the opening of the tubes 30 and
which then permit easy perforation of the thin central parts of the
portions in relief, the central parts forming the aforementioned
diaphragm 12.
The grooves 16 which are intended to cooperate with the grooves 15
are cut in those portions of the piece 19 limiting the recesses 18.
These grooves may be seen in FIG. 1 and are intended for the
venting of the tubes 30 when the ends of the conduit 9 penetrate
into these tubes.
FIG. 3 is a schematic view of a variant of an embodiment of FIG. 1.
In FIG. 3, the capillary conduits 9 that penetrate into the central
chamber 5 instead of being radially disposed as described with
respect to FIG. 1, are curved inward in the form of a spiral so as
to present greater capacities with a similar load or identical
capacities with a reduced load. In FIG. 3, the conduits 9 have
merely been schematized by the perforated lines which refer to the
capillar conduits. This form an disposition of the conduits 9 also
render possible for the centrifugation, the use of the force of
inertia at the start of the rotation of the displacement of the
columns of liquid to be distributed into the work tubes 30.
In an embodiment shown in FIG. 4, the liquid may be discharged from
the collection tube 1, not by pushing this tube 1 in the direction
of the arrow 11 of FIG. 1, but by screwing it onto the lower
central tube 2. This embodiment does not involve any modification
to the operating principle given with respect to FIG 1.
In FIG. 4, the lower central tube 2 has an outer thread 24 and the
collection tube 1 has an internal screw cutting 25. The outer
diameter of the central tube 2 is substantially equal to that of
the collection tube. The collection tube screws onto the central
tube 2 which acts as a plunger. The liquid is then pushed in
proportion to tube 1 into tube 2 which has a reduced internal
section in relation to that of tube 1.
In addition, FIG. 4 discloses the central tube 2 carrying a filter
26 on its lower part. It is obvious that the lower part of the tube
2 in the embodiment given in FIG. 1 may be provided with such a
filter.
In the method of operation given above, the filling of the conduits
9 and the measuring of the amounts of the liquid to be subsequently
transferred into the respective work tubes 30 are effected in two
stages corresponding to the pushing movement of tube 1 when the
central tube 2 penetrates further into tube 1 and to the retraction
of the central tube 2 from the collection tube 1.
FIG. 5 discloses another embodiment of the collection tube 1
permitting a simplified method of operation. In FIG. 5, the
collection tube 1 has a lower cylindrical part 41 and an upper part
42 which is frustoconical and then cylindrical with a larger
section than that of the lower part 41. The opening 43 of this tube
has the same diameter as that of the lower part 41. In the upper
part 42, the tube is fitted with blades 44 extending in such a way
as to align approximately the edges of the openings 43 and the wall
of the part 41. The lower part 41 is extended by a portion 45
forming the base of the tube. This portion 45 is slightly larger in
diameter than that of the part 41.
The drawing off and distribution member is similar to that shown in
FIG. 1. The lower central tube 2 has a constant transversal
cross-section. The end of this central tube which is intended to
penetrate into the collection tube 1 is fitted with a collar such
as 3 which forms the piston. The outer diameter of this collar is
substantially equal to that of the opening 43 or the part 41 of the
collection tube.
The lower control tube 2 may, as shown in FIG. 1, be provided with
guide blades such as 4.
When the piston of the lower central tube 2 is introduced into the
lower collection tube 1, the level of the liquid in the collection
tube and in the central tube rises to a level proportional to the
volume displaced. As soon as the collar forming the piston reaches
the part 41 having the smallest section, the volume of the liquid
contained in the part 45 is isolated from that contained in part
42. As the piston descends in part 41, the level of the liquid
descends in part 42, while under the influence of the generated
pressure the liquid in parts 41 and 45 makes the level rise in the
central tube 2 and then in the capillary conduits 9 and the central
tube 7. In the embodiment of the collection tube according to FIG.
5, the level of the part 41 is calculated so that the volume of
liquid displaced from the collection tube to the central tube 2 and
to the conduits 9 is allowed to pass the level of the throttling
means 10 of the conduits 9. The piston 3 continues to descend in
the collection tube and at the end of its course reaches the part
45 forming the base of the collection tube. As the piston is lower
in height than that of the portion 45, the collar 3 fitted on the
end of the central tube 2 no longer forms a piston which has the
effect of placing in contact the volumes of liquid contained in the
parts of the tube 1 on the one hand with the liquid of the collar 3
on the other. The level of liquid in the central tube 7 descends.
In the conduits 9, the level of liquid descends to the level of the
smallest section of the throttling means, while the liquid rises in
the collection tube as all the liquid flows from the central tube 7
of the central chamber 5 and the lower central tube 2. The measured
amounts of liquid are thus contained in the portions of the
conduits 9 lying between their ends opening into the central
chamber 5 and the throttling means. The collection tube can then be
centrifuged with the unit comprising the conduits 9. The edge at
the level of the opening prevents overflow of the liquid during the
distribution of the measured amounts of liquid into the respective
work tubes.
FIG. 6 shows another variant of an embodiment of the collection
tube 1 and the member 20 for the drawing of measured amounts of
liquid.
The collection tube 1 is similar to that shown in FIG. 5. It
comprises a lower cylindrical part 51 having a smaller section than
upper part 52. The lower part of the part 51 bears the base of the
collection tube. The base of th tube comprises a substantially
conical stud 56 disposed substantially in its center. The part 52
is cylindrical at the level of the opening 53 of the tube and
frustoconical to permit connection with the part 51 having an upper
section smaller than part 52. Part 52 is also provided with blades
54 extending towards the inside of the tube and coming into
alignment with the walls of the part 51.
The member 20 comprises the capillary conduits 9 but in this
embodiment as compared to that of FIG. 1, the conduits 9 are bent
at 69 such that the lower part of each of these is vertical. All
the lower vertical parts of the conduits 9 extend to the end of the
central tube 62 (analogous to the central tube 2 of FIG. 1). At its
lower end, the group of vertical parts of the conduits 9 is
provided with a collar 63 having an outer diameter which is
substantially equal to the diameter of the cross-section of part 51
of the collection tube, whereas the lower end of the central tube
62 is closed by a sealed diaphragm 64.
The second end of the central tube 62 is in contact with the
atmosphere. For this reason, the second end of the tube 62 emerges
either in the lower part 67 of the group of median parts of the
conduits 9 or in an annular conduit which is also open and which is
located in the inner space formed by the median parts of the
conduits 9.
As in the case of the embodiments described above, the collar 63 is
introduced into the collection tube 1. As soon as it reaches the
part 51, having a constant but smaller section than that of the
part 52, it adopts the role of a piston. The liquid imprisoned in
the part 51 below the piston rises in the conduits 9 under the
influence of the pressure as the collar 63 descends in the tube 1.
The volume of the part 51 of the collection tube is selected so
that it is equal or slightly greater than the sum of the volumes of
the liquid in the parts of the capillary conduits lying between
their lower ends and the throttling means 10.
At the end of the stroke of the piston 63 in the part 51 of the
collection tube, the conical bussing 56 pierces the diaphragm 64
initially sealing the central tube 62. The columns of liquid in the
conduits 9 thus come into contact with the atmosphere. The level of
liquid descends in the conduits 9 to the level of the throttling
means 10. The collection tube is then withdrawn and the group 20 is
then centrifuged to obtained distribution of the measured amount of
the liquid in the work tubes.
In the above embodiment, the collection tube and/or the retention
member 20 of the measured amounts of liquid and/or the work tubes
are preferably of transparent plastic materials. The work tubes may
contain initially reagents in the form of a liquid or solid or
absorbed, for example, in blotting paper, to allow for direct
analysis of the liquid distributed in the work tubes by direct
examination of the colored reactions or by comparing these
reactions with a colored screen.
In the embodiment of FIG. 7, the work tube 117 comprises two
superimposed compartments 101 and 102 closed respectively by a
piece 105 fitted with a pierceable diaphragm 106 and a piece 103
fitted with a pierceable diaphragm 104.
The pierceable diaphragm 106 is disposed, for example, at the
bottom of a cylindrical hole 112 made in the piece 105.
The conduit 100 comprises a channel 107 and is bent at 113 at the
level of its end, towards the outside of the retention unit. The
end of the channel 107 discharges through a conical point 108
disposed facing the work tube 117.
The conical point 108 is provided with a neck 109, which is
preferably sharp. The neck 109 of the point 108 is intended to
ensure the venting of the upper compartment 101 and the bringing
into contact of the compartment 101 with the adjacent compartment
102.
The superimposed cross-piece 110 and 111 are applied between a
plane face 118 of the conduit 100 and a plane face of the piece 105
to limit the course of the piece 108 and to keep the neck 108 at
predetermined levels in the work tube 117.
According to a preferred embodiment, the cross-piece 111 and 110
are common to all the tubes 117 of the manifold distributor and
consist of circular bands which may be removed at the time of use
by a circular movement. Each of the bands may be provided with a
part which is not shown but which projects outward to facilitate
gripping the band.
The implementation of a device according to the invention is
described below with reference to FIGS. 8, 9 and 10.
Compartment 101 is first partially filled with a first reagent 114
and compartment 102 with a second reagent 115 (see FIG. 8).
The plane face 118 of the conduit 100 abuts against the
cross-pieces 110 and 111 and the end of the conical point 108 is
disposed slightly above the diaphragm 106 closing the compartments
101.
In a first phase (see FIG. 9), the cross-piece 111 is removed and
pressure is applied to the bend 113 in the direction of the arrow
120. The face 118 of the conduit 100 abuts on the cross-piece 110.
The end of the conical point 108 tears the diaphragm 106 and
penetrates into compartment 101. The beveled neck 109 ensures
venting of this compartment.
In a second stage the liquid contained in the channel 107 is
injected by centrifuging according to arrow 121 into compartment
101 and is mixed with the reagent 114. 122 is the resultant mixture
thus obtained.
In a third stage, for example, after a period of incubation of the
mixture 122 at a given temperature, the cross-piece 110 is removed
(see FIG. 10) and pressure is applied to the bend 113 in the
direction of the arrow 130. The face 118 comes to rest against the
upper face of the piece 105. The end of the conical point 118 tears
the diaphragm 104 and penetrates into compartment 102.
The neck 109 102 ensures contact between the two compartments. The
mixture 122 flows in the direction of the arrow 131 into the
reagent 115. The new mixture obtained is designated by 132. The
tube 107, which is now empty of liquid, ensures venting of the
compartment 102.
A particular embodiment of the device according to the invention
has been described.
Naturally, the work tube 117 may comprise any number of
superimposed compartments connected, for example, to a plurality of
cross-piece. The reagents may be in liquid or solid form.
Various modifications can be accomplished in the present invention
by those skilled in the art and accordingly the scope of the
present invention shold be measured solely from the following
claims.
* * * * *