U.S. patent number 3,756,458 [Application Number 05/006,440] was granted by the patent office on 1973-09-04 for burettes and pipettes.
Invention is credited to Matzas Alfonso Fill.
United States Patent |
3,756,458 |
Fill |
September 4, 1973 |
BURETTES AND PIPETTES
Abstract
Method and device for dispensing a predetermined amount of
liquid which includes a storage means for holding liquid therein, a
nozzle for dispensing the liquid from the storage means, and means
for producing a symmetrical puff of gas about the nozzle side wall
to remove droplets from the outlet of the nozzle.
Inventors: |
Fill; Matzas Alfonso (Sussex,
Brighton, EN) |
Family
ID: |
9800146 |
Appl.
No.: |
05/006,440 |
Filed: |
January 28, 1970 |
Foreign Application Priority Data
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Feb 3, 1969 [GB] |
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5,655/69 |
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Current U.S.
Class: |
222/1; 222/631;
222/632; 222/108; 422/919 |
Current CPC
Class: |
B01L
3/02 (20130101); B01L 2200/0615 (20130101); B01L
2400/02 (20130101) |
Current International
Class: |
B01L
3/02 (20060101); B65d 083/00 () |
Field of
Search: |
;222/1,192,193,195,4,148,420,108 ;259/147,151 ;34/85
;128/215,218R,218C |
References Cited
[Referenced By]
U.S. Patent Documents
Primary Examiner: Reeves; Robert B.
Assistant Examiner: Bartuska; Francis J.
Claims
What we claim is:
1. A method of delivering a measured quantity of liquid from a
burette, pipette, syringe or the like each of which includes
storage means for storing a quantity of liquid, nozzle means
coupled to said storage means, said nozzle means directed
vertically downwards and having an outer side wall and a central
bore with an outlet at the terminus of the bore, and means for
dispensing a measured amount of liquid to be dispensed from said
storage means through the central bore of said nozzle, the method
comprising the steps of providing an annular chamber about the
nozzle, said annular chamber having an inwardly disposed
cylindrical wall extending about the nozzle and spaced therefrom
and which extends towards said outlet but not below said outlet,
the chamber only permitting the escape of gas from the chamber in
the direction of the outlet and about the nozzle outer side wall;
dispensing liquid from said storage means through said nozzle
outlet; discontinuing said dispensation of liquid; and then
directing a puff of compressed gas into the chamber to produce a
symmetrical puff of gas flow between the outer side wall of the
nozzle and the inwardly disposed cylindrical wall to discharge
liquid adhering to the nozzle and permit its passage vertically
downwards.
2. The method of claim 1 in which a hand operated bulb is coupled
to the annular chamber and is squeezed to produce the puff of
gas.
3. A device for delivering measured amounts of liquid comprising
storage means for storing a quantity of liquid, nozzle means
coupled to said storage means, said nozzle means having an outer
side wall and a central bore with an outlet at the terminus of the
bore, means for dispensing a measured amount of liquid from the
storage means through the central bore of said nozzle, means for
measuring the quantity of liquid dispensed, a hollow cylindrical
member having an inner side wall, said cylindrical member, inner
side wall and said nozzle outer side wall defining an annular
chamber, said annular chamber extending about the nozzle outer side
wall and spaced therefrom and which also extends towards the outlet
of the nozzle but not below the outlet of the nozzle, said annular
chamber only permitting the escape of gas from the chamber in the
direction of the outlet and about the nozzle outer side wall, a
tube extending into the annular chamber, and means for producing a
puff of compressed gas coupled to said tube in order to produce a
symmetrical puff of gas flow between the outer said wall of the
nozzle and the inner side wall of the hollow cylindrical member to
effect removal of liquid adhereing to the nozzle outlet.
4. The device according to claim 3 in which the storage means,
comprises a syringe, the means for dispensing comprises a syringe
plunger and the nozzle comprises a needle, and in which the
cylindrical member surrounds and is supported by a portion of the
syringe.
5. A device according to claim 4 in which the needle extends past
the end of the hollow cylindrical member.
6. A device according to claim 5 in which a valve is provided in
the tube to only permit the flow of gas into the annular
chamber.
7. A device according to claim 3 in which a hand operable bulb is
coupled to the tube.
8. A device according to claim 3 in which the cylindrical member
surrounds and is supported by a portion of the nozzle.
9. A device according to claim 8 in which the nozzle extends past
the end of the hollow cylindrical member.
10. A device according to claim 9 in which a valve is provided in
the tube to only permit the flow of gas into the annular
chamber.
11. A device according to claim 3 in which the nozzle includes a
chisel edge at the end thereof about the outlet.
Description
The present invention relates to burettes, pipettes, syringes and
the like.
In the delivery of accurately measured quantities of liquid from a
burette, pipette, syringe or the like, one of the limits of
accuracy has always been that the liquid cannot be dispensed in
units of less than one drop, the size of which will depend on the
liquid being delivered, and the material and configuration of the
delivery nozzle.
It is an object of this invention to provide a method and apparatus
by means of which the accuracy of measurement of dispensing a
liquid from an orifice can be greatly increased. This object is
achieved by a method for removing droplets from the tip of the
delivery nozzle of burettes, pipettes, syringes and the like. It is
a further object of the invention to provide a method for stirring
a liquid into which the tip of the delivery nozzle of a burette,
pipette, syringe or the like, projects.
Accordingly the invention provides a method for removing droplets
from the tip of the delivery nozzle of burettes, pipettes,
syringes, and the like, or for stirring liquid into which the tip
of said delivery nozzle projects, which comprises directing a puff
of gas past said tip in the direction of delivery of liquid through
said nozzle in such a manner as to symmetrically surround said tip.
This method is effected by a device having a gas passage therein,
an inlet to said passage adapted to be supplied with pressurised
gas, and an outlet directed in the delivery direction of said
delivery nozzle and arranged to allow egress of said pressurised
gas adjacent to and symmetrically surrounding the tip of said
delivery nozzle.
The method is performed by applying a short flow or puff of gas
after the main delivery of the liquid has been completed, the flow
or puff of gas being of sufficient intensity to blow off
substantially all adhering liquid. The effect is to take the level
of the liquid at the delivery nozzle always to a definite level.
Thus, a puff of gas before delivery effects an adjustment of the
liquid to this definite level, and a puff after a measured volume
has been dispensed returns the liquid to the same level. The volume
of liquid which has passed out of the apparatus thus accurately
corresponds to the volume measured and no allowance or corrections
have to be made for liquid adhering to the nozzle.
A preferred embodiment of the device of the invention comprises a
cylindrical member for mounting coaxial with the delivery nozzle,
to be spaced therefrom so as to provide an annular chamber, the end
of the cylindrical member remote from the tip of the delivery
nozzle fitting the nozzle tightly. The cylindrical member is
conveniently provided with a side-tube as the inlet through which
the gas can be applied, and has an outlet adjacent to and
symmetrically surrounding the tip of the delivery nozzle. The
side-tube preferably incorporates a valve, thereby preventing any
suck-back of the gas after a puff, and it is convenient for the
valve to be a press-button valve which is operated each time a puff
of gas is required. The tip of the delivery nozzle of any burette,
pipette, syringe or the like, forming part of the device, or to
which the device is attached, is most desirably provided with a
chisel-edge, and is preferably coplanar with the outlet of the
cylindrical member. When the tip of the delivery nozzle extends
beyond the outlet, the incorporation of a valve into the side-tube
is not necessary for maximum effectiveness and accuracy.
An alternative embodiment is one in which instead of an annular
chamber around the delivery nozzle constituting the gas passage,
the gas passage is contained within the walls of the cylindrical
member, and thus the device need not touch the delivery nozzle.
The gas used in operation of the device of the invention may be
air, and the air can be applied conveniently from a source of
compressed air such as a hand-operated bulb or syringe. Other
preferred gases are nitrogen, carbon dioxide, or an inert gas such
as argon. Sterile air may also be used when it is desirable to
operate the device under sterile conditions.
The device of the invention is particularly useful in combination
with a micrometer syringe, such as that sold under the registered
trade-mark "Agla." In this case the delivery nozzle comprises a
syringe needle, around which the cylindrical member is positioned.
The capillary of the needle is of a very fine bore, and the finer
the bore, the greater is the gas pressure require to remove the
droplets; conversely, if the syringe is fitted with a shorter
needle, the gas pressure required is decreased.
The invention therefore provides a means of obtaining a very high
order of accuracy of dispensing liquid in a microtitration,
particularly for volumes of liquid less than 500 microlitres. When
a microtitration is performed the needle is cleared initially with
a puff of gas; when the microtitration is performed with the needle
above the surface of the solution in the receiving vessel, each
drop of liquid delivered can be blown off by subsequent discharges
of the gas. Thus liquid can be dispensed under sterile conditions,
and the need for any contact with the sides of the receiving vessel
is obviated.
The invention also provides a means of dispensing accurate volumes
of liquid in chromatography procedures. Preferably the needle is
held close to, but not in contact with, the support medium.
As well as its use in connection with syringes, the device of the
invention provides a means for increasing the accuracy of
dispensing liquid from a burette, pipette or the like. In this
case, the device is most conveniently constructed as an adaptor for
sliding onto the conical delivery nozzle of the measuring
device.
The invention also provides a means for stirring a liquid, the flow
of gas through the liquid or onto the surface simulating mechanical
agitation.
In order that the invention may be more fully understood, preferred
embodiments thereof, which are not to scale, will now be described,
by way of illustration only, with reference to the accompanying
drawings in which:
FIG. 1 is a vertical cross-section of a syringe in combination with
a device according to the invention;
FIG. 2 is a cross-section of the tip of the syringe shown in FIG.
1;
FIG. 3 is a vertical cross-section of a burette in combination with
an alternative form of a device according to the invention;
FIG. 4 is a vertical cross-section of a device according to the
invention, similar to that shown in FIG. 3, showing its use as a
stirring mechanism.
Referring first to FIG. 1, there is shown a syringe 1, fitted with
a plunger 2 and a needle 3, in combination with a cylindrical
member 4 forming an annular chamber 5. Chamber 5 is provided with
an inlet 6 connected to a side-tube 7 fitted with a valve 8.
Side-tube 7 is connected to a hand-operated bulb 9 of compressed
air by rubber tubing 10. Chamber 5 has an outlet 11 adjacent to and
surrounding the tip 12 of the syringe needle 3.
In operation, air is supplied from the bulb 9, the supply being
controlled by the valve 8 in the side-tube 7. The air passes
through the inlet 6 into the chamber 5, and hence through the
outlet 11 removing liquid adhering to the tip 12 of the syringe
needle 3.
The syringe 1 can be positioned in a horizontal plane, in which
case the cylindrical member 4 and the syringe needle 3 may be
extended and curved so that the tip 12 of the needle 3 is
directedly vertically for delivery. It is often convenient to use a
straight needle 3, fitted to the syringe 1 by means of a curved
metal tube.
FIG. 2 shows the tip 12 of the syringe needle 3, the tip 12 having
a chisel-edge 13 to facilitate the removal of adhering liquid.
In FIG. 3, the conical-shaped delivery nozzle 14 of a burette 15 is
surrounded by a coaxial member 16. The upper end of the coaxial
member 16 has a tapered opening adapted to fit precisely onto the
tapered delivery nozzle 14. The same reference numerals as those
used in FIG. 1 and 2 are employed here where applicable. The
side-arm 7 is connected by rubber tubing 10 to a source of
pressurised gas (not shown in this Figure).
FIG. 4 shows the delivery nozzle 17 of a measuring device (not
shown in this Figure), the nozzle 17 projecting into a liquid 18 is
contained in a vessel 19.
In operation a flow of gas passing through the outlet 11 of the
chamber 5 causes agitation of the liquid 18, and thus effects
stirring.
* * * * *