U.S. patent number 3,754,687 [Application Number 05/182,469] was granted by the patent office on 1973-08-28 for a multi-positional pipetting dispenser.
This patent grant is currently assigned to Kontes Glass Company. Invention is credited to Robert W. Norton.
United States Patent |
3,754,687 |
Norton |
August 28, 1973 |
A MULTI-POSITIONAL PIPETTING DISPENSER
Abstract
A pipetting dispenser of the piston-cylinder type with
interchangeable fittings by which the operative movement of the
piston may be arranged to be in line with or at an angle to the
axis of an attached pipette and having adjustable stop means to
limit travel of the piston within predetermined limits, together
with means automatically retracting the piston from its discharge
position to a position in engagement with said adjustable stop
means, thereby insuring the repeated dispensing of like volumes of
liquid from said pipette in successive operations of the dispenser,
for any adjusted position of the stop means.
Inventors: |
Norton; Robert W. (Vineland,
NJ) |
Assignee: |
Kontes Glass Company (Vineland,
NJ)
|
Family
ID: |
22668624 |
Appl.
No.: |
05/182,469 |
Filed: |
September 21, 1971 |
Current U.S.
Class: |
73/864.14;
73/864.18; 604/211; 422/925; 222/309; 604/218 |
Current CPC
Class: |
G01F
11/06 (20130101); B01L 3/0224 (20130101) |
Current International
Class: |
B01L
3/02 (20060101); G01F 11/06 (20060101); G01F
11/02 (20060101); G01f 011/06 () |
Field of
Search: |
;222/1,14,31,43,50,250,309,384,400.8,386 ;73/425.4P,425.6
;141/25,26,27 ;128/213,224,234,235,236,237,238,239 ;239/587
;138/89,92 ;124/13 |
References Cited
[Referenced By]
U.S. Patent Documents
Primary Examiner: Reeves; Robert B.
Assistant Examiner: Rolla; Joseph J.
Claims
What I claim as new is:
1. A pipetting dispenser comprising a body member having a cavity
therein, a plunger mounted within said cavity for reciprocation
therein, means defining fluid inlet and discharge passage means in
said body member in communication with said cavity, means for
reciprocating said plunger within said cavity to draw fluid into
said cavity by movement thereof in one direction and to discharge
fluid from said cavity by movement in the opposite direction
adjustable stop means for variably limiting the plunger stroke in
one direction and fixed stop means limiting the plunger stroke in
the opposite direction means to support a pipette in operative
fluid communication with said cavity in said body member, said
pipette-supporting means including a body-member-attachment fitting
having communicating fluid bores therein, each bore having fluid
communication at one end with a pipette supported in said pipette
surrpot, each of said bores opening at their opposite ends out
through distinct and spaced-apart wall portions of said body
member, said body member having mounting means operatively and
selectively connectable to either one or the other of said bore
openings, and a closure plug for closing the other of said bore
openings not connected to said mounting means.
2. The apparatus as described in claim 1 wherein both the fixed
stop means and said adjustable stop means are disposed within the
cavity in said body member, and wherein means are provided for
resiliently biasing said plunger to a normally retracted position
in engagement with said adjustable stop means.
3. The apparatus as described in claim 1 wherein said adjustable
stop means comprises a sleeve threadedly mounted in the wall of
said cavity in coaxial relation thereto and provided with a stop
shoulder adjustably positionable within said cavity in the path of
movement of said plunger.
4. The apparatus described in claim 3 wherein a threaded jam nut is
provided and engaged with said sleeve to lock the sleeve in an
adjusted position within said body member.
5. The apparatus as described in claim 1 wherein said pipette
supporting means includes a theaded terminal portion on said
body-member-attachment fitting provided with a recess dimensioned
to receive an end portion of a pipette, and a screw-threaded
packing gland engaged with said terminal portion for compressing a
packing material within said gland into sealing engagement with a
pipette fitted into said recess.
6. The apparatus as described in claim 1 wherein calibrated indicia
are provided on said dispenser to indicate the effective volume of
the cavity as defined by said adjustable stop means.
Description
This invention relates generally to pipetting dispensers and in
particular to pipetting dispensers of the piston-cylinder type.
One object of the invention is to provide a pipetting dispenser of
the character described wherein means are provided in the form of
interchangeable fittings by which piston travel may be arranged to
be in line with or at an angle to the axis of the dispenser
pipette.
Another object of the invention is to provide in a pipetting
dispenser of the character described, adjustable stop means for
limiting piston travel within the cylinder to adjust the effective
volume of the cylinder, within predetermined limits, as determined
by the extent of piston travel.
A further object of the invention is to provide in the dispenser of
the character described, means to retract the piston after each
discharge stroke thereof to a position in engagement with the
adjustable stop means whereby to accurately measure and repeatedly
discharge successive equal amounts of liquid from the
dispenser.
Another object of the invention is to provide lock means for
maintaining the adjustable stop means in an adjusted position
whereby to insure the discharge of accurately measured amounts of
liquid by repeated and successive operations of the dispenser.
A further object of the invention is to provide means for attaching
and replacing a pipette in operative position on the dispenser.
Another object of the invention is to provide a reliable pipetting
dispenser for use with glass disposable pipettes as well as with
standard laboratory pipettes.
A further object of the invention is to provide a repetitive
pipetting dispenser which can be completely autoclaved.
A further object of the invention is to provide a pipetting
dispenser in which the material being dispensed does not come in
contact with the body of the dispenser but is always contained
within the glass pipette attached thereto.
Another object of the invention is to provide a pipetting dispenser
of the character described which can be used to eject the cotton
plugs laboratory workers insert in the large or "mouth" end of
pipettes to prevent mouth contamination by the material being
pipetted when the last volume of material in the pipette is "blown
out" as is customary.
These and other objects of the invention will become apparent from
the following specification and drawings wherein I have described
and illustrated a preferred form of my invention and wherein:
FIG. 1 is a side elevation of the invention with the parts thereof
arranged so that the line of piston travel is disposed at an angle
to the axis of the pipette,
FIG. 2 is an enlarged sectional view of the dispenser with the
parts thereof arranged as shown in FIG. 1,
FIG. 3 is a side elevation of the invention with the parts thereof
arranged so that the line of piston travel is aligned with the axis
of the pipette, and
FIG. 4 is a view similar to FIG. 2 showing the dispenser arranged
to eject a cotton plug from a pipette.
Referring to the drawings more particularly, the invention is shown
comprising a body member 1 provided with an inner cylindrical
cavity 2 opening out through one end of said body member and within
which a piston or plunger 3 is disposed with the cavity 2 and the
plunger 3 accurately dimensioned to permit longitudinal movement of
the plunger within the cylindrical cavity. The plunger 3 may be
provided with a packing groove 4 within which is disposed a sealing
ring 5 whereby to preclude leakage of the liquid being dispensed
past the plunger.
The plunger 3 is provided with an operating rod 6 which may be
threadedly attached to the right-hand end of the plunger as viewed
in FIG. 2. The rod 6 is of a length to extend beyond the open end
of the body which end terminates in an operating handle or button 7
by which the plunger may be reciprocated within the cylinder. The
inner surface of the wall of the cylindrical cavity 2, at the open
end thereof, and through which the operating rod 6 projects, is as
shown, provided with an internal screw thread 8. An exteriorly
screw-threaded sleeve 9 is threadedly engaged with the threaded
portion 8 of the body 1 and the sleeve 9 is provided with a through
longitudinally extending bore 10 dimensioned to receive and guide
the rod 6 in its operative movements. The inner end 11 of the
sleeve 9 is designed to function as an adjustable stop means to
limit the travel of the plunger in one direction within the
cylindrical cavity 2 and to this end has a radial wall thickness
such as to overlap radially the transverse dimension of the plunger
3 as clearly shown in FIG. 2. Thus, by adjusting the sleeve
longitudinally of the cylindrical cavity 2, the permissible travel
of the plunger 3 to the right as viewed in FIG. 2 is adjustably
defined, and thus the volume of the liquid charge drawn into that
portion of the cavity 2 indicated by the reference 12 may be
accurately determined. A jam nut 13 is provided on that portion of
the sleeve 9 projecting beyond the body 1 to lock the sleeve 9 in
adjusted position and thus insure accuracy in the volumetric amount
of liquid dispensed in successive operations of the plunger 3 in
any given position of adjustment of said sleeve. A coil spring 14
surrounds the projecting end of rod 6 and is placed under proper
compressive force between the button 7 and the adjacent end of
sleeve 9 to resiliently bias the plunger 3 into engagement with
stop 11 and thus insure full retraction of the plunger 3 upon its
release, following a full discharge stroke toward the left as shown
in FIG. 2.
The opposite end of the body 1 is provided with mounting means
comprising a reduced externally threaded portion 15 having a
through central bore 16 in communication with the portion 12 of the
cavity 2, by tapered section 16a which is dimensioned to provide
fixed stop means to limit travel of the plunger 3 towards this end
of the body member and thus in cooperation with the adjustable stop
means 11, define the range of movement of the plunger 3 within the
cavity 2. The terminal portion 15 of the body 1 is screw-threadedly
attached to a body-member-attachment fitting 17 having a threaded
bore 18 opening laterally of said fitting. The bore 18 thus
communicates with a longitudinally extending through bore or
channel 19 in the fitting 17. The upper end 20 of said fitting is
provided with a threaded bore 21 of the same dimensions as that of
the bore 16, whereby a screw-threaded plug 22 is interchangeable
with the body member 1 in respect to the bores 16 and 21 so as to
provide for the different arrangements of these parts as shown in
FIGS. 1 and 3. This will allow the dispenser to be operated in
areas where space limitations are restrictive.
The opposite end 23 of the fitting 17 is provided with
pipette-supporting means for detachably connecting a pipette
thereto, thus the end 23 is exteriorly threaded as shown to
threadedly receive a screw-threaded packing gland 24 which serves
to secure and maintain a fluid-tight seal between the packing seal
25 and the upper end of a pipette 26 fitted within the lower
enlarged end of the channel 19.
The dispenser may be provided with calibrated indicia to indicate
the volumetric contents of the portion 12 of the cavity 2 as
determined by the setting of the stop member 11, and to this end
calibrated indicia 28 may be inscribed on the wall of the body 1
when it is made of transparent or translucent plastic material as
hereinafter suggested.
With the parts arranged for example as shown in FIG. 3, the
operation of the dispenser is as follows: the operator depresses
the rod 6 against the force of the spring 14 until the plunger 3
encounters the lower stop means 16a. The free end of the pipette 26
is immersed within a body of liquid to be pipetted and the plunger
rod 6 then released whereupon the spring 14 retracts the plunger 3
into a position where it is engaged with the stop 11 which has been
previously adjusted to define a predetermined volume within the
portion 12 of the cavity 2. The dispenser is then positioned in
discharging relation to the vessel or container into which the
liquid is to be dispensed, and the plunger rod 6 depressed to
discharge the liquid from the chamber 12. Repeated operation of the
dispenser as described insures accurately measured volumes of the
liquid being dispensed on successive operations thereof, for each
adjusted position of the stop member. These operations are
facilitated by the provision of the shaped finger piece 27 which
may be press fitted to the body member 1 adjacent the upper end
thereof. The finger piece may be engaged by the operator's first
and second fingers while he presses the plunger rod 6 with his
thumb.
Reference has been made herein to the fact that the material being
dispensed does not come into contact with the body of the dispenser
and to this end the plunger 3, the cavity 12, and the plunger
travel are dimensioned and proportioned so that the maximum
volumetric capacity of the cavity 12 as determined by the position
of the plunger 3 never exceeds the volume of the pipette attached
to the dispenser. Thus the material being pipetted never enters the
body of the dispenser, but remains contained within the body of the
pipette.
The dispenser may be made of metal, glass, or plastic material such
as Nylon and the body member may be transparent or translucent so
that the plunger 3 may be properly oriented within the cavity 2 as
indicated by the calibrations 28.
Reference has been made herein to the use of the dispenser unit of
my invention as a means of ejecting the cotton plugs which
laboratory workers usually insert in the large or mouth end of the
standard pipette, to prevent contamination by the material being
pipetted when the last volume of material in the pipette is "blown
out." These plugs are generally removed by the use of wires, hooks,
tweezers, etc., and the operation is sometimes quite troublesome,
and is always a nuisance. With the dispenser of my invention, the
cotton plugs can readily be ejected by wetting or soaking the
"plug" with water, and inserting the tip end of the plugged pipette
in the lower end 23 of the dispenser with a snug or air-tight fit
and then rapidly depressing the plunger handle 6, whereupon the
resulting air pressure built up in the chamber 19 between the
plunger and the pipette tip readily ejects the soaked plug.
Having thus described my invention, it is apparent that I have
provided a pipetting dispenser which provides means for realizing
the several objects hereinbefore noted, and that the dispenser is
simple in construction and reliable in operation.
* * * * *