U.S. patent number 3,753,657 [Application Number 05/152,189] was granted by the patent office on 1973-08-21 for automatic test tube transporter and sample dispenser having solid state controls.
This patent grant is currently assigned to Micromedic Systems, Inc.. Invention is credited to Harvey T. Downing, Charles V. Lawson, Byron E. Sturgis.
United States Patent |
3,753,657 |
Downing , et al. |
August 21, 1973 |
**Please see images for:
( Certificate of Correction ) ** |
AUTOMATIC TEST TUBE TRANSPORTER AND SAMPLE DISPENSER HAVING SOLID
STATE CONTROLS
Abstract
An automatically controlled test tube transporter apparatus
which advances a test tube rack containing two rows of receptacles
for test tubes under a vertically movable aspirating and dispensing
tip or nozzle which is adapted to move down into adjacent tubes and
aspirate or discharge depending on the test to be performed. The
tip is operationally in communication with a twin pipette metering
and dispensing apparatus which includes a linearly adjustable
eccentric mechanism which drives the pipette pistons with a motion
of adjustable sinusoidal amplitude. The apparatus includes a solid
state control circuit which includes a binary shift register. The
pipettes may be adjusted to either work in parallel or alternating
strokes. An automatic tip wiping mechanism, controlled by the
operation of the tip, is provided to insure precision in the
processing. The apparatus is adapted to be set for continuous
operation or for individual test tube processing cycle operation.
Empty racks can be advanced without engaging the tip and pump
apparatus. The pipetting mechanism may be operated separately
without the operation of the transporting mechanism. A malfunction
and alarm logic circuit gives a warning in case of certain
malfunctions and stops the apparatus.
Inventors: |
Downing; Harvey T. (Huntsville,
AL), Lawson; Charles V. (Arab, AL), Sturgis; Byron E.
(Huntsville, AL) |
Assignee: |
Micromedic Systems, Inc.
(Philadelphia, PA)
|
Family
ID: |
22541872 |
Appl.
No.: |
05/152,189 |
Filed: |
June 11, 1971 |
Current U.S.
Class: |
422/65;
73/863.32; 73/864.16; 73/864.22; 73/864.24; 141/130 |
Current CPC
Class: |
G01N
35/10 (20130101); B01L 9/06 (20130101); G01N
35/1004 (20130101) |
Current International
Class: |
B01L
9/00 (20060101); B01L 9/06 (20060101); G01N
35/10 (20060101); G01n 021/00 () |
Field of
Search: |
;23/230,253,292,259
;141/130 |
References Cited
[Referenced By]
U.S. Patent Documents
Primary Examiner: Wolk; Morris O.
Assistant Examiner: Serwin; R. E.
Claims
We claim:
1. An apparatus for selectively or automatically processing racks,
each having a row of liquid sample-containing tubes and a row of
additional tubes either empty or containing suitable re agents,
said rows being adjacent so as to form pairs of tubes, said
apparatus comprising a base member having a supporting surface
divided into a rack-loading area to receive loaded racks for
processing, a rack-advancement area, and a rack-ejectment area,
rack-advancement means adjacent said rack-advancement area to
advance a rack along a linear path from an initial position
adjacent the loading area to a final position adjacent the
ejectment area, liquid sampling and dispensing means, conduit means
in communication with said sampling and dispensing means, an
aspirating and dispensing tip means above the path and connected to
said conduit means, said rack-advancement means being constructed
and arranged to advance said racks incrementally to position
successive pairs of tubes under said tip means, tip-moving means
comprising means for shifting the tip means to and fro between two
positions corresponding to those of a pair of the tubes in a rack,
and means for raising and lowering the tip means in a straight line
at the two positions, rack-ejection means adjacent said final
position for ejecting a processed rack from said path in a
direction transverse to said path into said rack-ejectment area,
means for feeding the racks in the loading area successively into
the initial position of the path by moving said racks transverse to
said path in a direction opposite that of ejectment, and control
means for coordinating the operation of said liquid sampling means
and dispensing means, said rack-advancement means, said
tip-shifting means, said tip raising and lowering means, and said
ejection means.
2. An apparatus as in claim 1 wherein said tip-shifting means
includes a driven grooved cylindrical cam means and a cam groove
follower means adapted to ride in a groove of the cam means to
control the position of said tip means.
3. An apparatus according to claim 2 in which the cylindrical cam
means has two peripheral cam grooves and the follower means
comprises a separate follower for each cam groove and a snap-action
lever, connected at each of its ends to a separate one of the
followers, for alternately bringing a follower into its respective
groove, each groove having a portion which raises the follower
therein and rocks the lever to its snap-action position, thereby
causing the other follower to engage the other groove.
4. An apparatus as in claim 3 including a bidirectional tip motor,
said control means adapted to rotate said motor in either
direction, one direction of rotation adapted to move said tip
downwardly and the other direction of rotation adapted to move said
tip upwardly.
5. An apparatus as in claim 4 and further including a switch cam
having a pair of lobes located adjacent said cylindrical cam, said
cylindrical cam having an actuator bar thereon which is adapted to
engage one of said lobes upon rotation in either direction.
6. An apparatus as in claim 1 wherein said tip-moving means
includes a pair of guide rods and a cam means to move said guide
rods longitudinally to a first and second position, tip holder
means mounted on said guide rods, said tip means being located in
said tip holder means, and a cross slide assembly means associated
with said tip holder to move said tip holder and said tip means
down and up along said guide in both said positions.
7. An apparatus as in claim 6 and including a drive means for
rotating said cam means, a crank means associated with said drive
means and operatively in engagement with said cross slide means to
effect downward and upward displacement of said cross slide means
and said tip holder when said cam means is rotated.
8. An apparatus as in claim 7 and including a cam follower toggle
means, said toggle means having two cam followers and said cam
having two cam grooves therein, said toggle means adapted to toggle
when one of said cam followers rides out of one of said cam grooves
and thus to force the remaining cam follower into the other cam
groove.
9. An apparatus as in claim 7 wherein said crank means has a top
dead center cam on one end thereof, a switch means, said top dead
center cam means adapted to engage said switch means when said
cross slide assembly and said tip holder are in a predetermined
position for either aspirating or dispensing.
10. An apparatus as in claim 1, and including a rack bar means
adapted to bias said racks into advancement position on said
surface means.
11. An apparatus as in claim 1 wherein said liquid sampling and
dispensing means comprises a pair of pump units, each pump unit
having a piston and a common driving means for driving the pistons
of each said pump unit in a sinusoidal reciprocal manner, each pump
unit having at least two port bores thereon and valve means
associated with said common driving means and said port bores to
alternately place the chamber of each pump unit in communication
with one of said port bores respectively.
12. An apparatus as in claim 1 and additionally including means for
wiping said tip means as it is moved upwardly by said tip raising
and lowering means in each position.
13. An apparatus as in claim 12 wherein said wiper means includes a
supply reel of wiper tape and a take-up reel for the wiper tape and
a driving spool means for advancing the wiper tape.
14. An apparatus as in claim 13 wherein said tip wiper means also
includes a pair of pad means and means to press said wiper tape
against the sides of said tip means as it is being raised in either
position to effect a wiping thereof.
15. An apparatus as in claim 13 wherein said wiper spool driving
means includes a plurality of peripheral projections adapted to
penetrate said wiper tape.
16. An apparatus as in claim 12 including a drive means for
operatively effecting rotation of said wiper spool drive means upon
upward motion of said tip means.
17. An apparatus as in claim 1 wherein said control means includes
a malfunction and alarm circuit means, said malfunction alarm
circuit means including a buzzer which is adapted to sound for a
predetermined period of time upon the completion of processing and
then remain silent for a second period of time and again sound for
said first predetermined period of time whereby said cycle is
repeated until an operator shuts off the apparatus.
18. An apparatus as claimed in claim 1 including wiper means for
wiping said tip during its upward movement out of said tubes, said
wiper means including a supply reel of wiping tape, and said
control means having a circuit means to prevent said apparatus from
functioning if there is no supply reel in place.
19. An apparatus as in claim 1 and including wiper means to wipe
said tip, said wiper means being mounted on a tray or drawer which
may be pulled outwardly from the apparatus into open position for
servicing, said control means including a malfunction circuitry
means adapted to shut down the operation of the machine if the tray
is in open position or if a jam occurs in the rack advancement
means.
20. An apparatus as in claim 19 wherein said wiper means includes a
reel supply of tape, and said control means has a photoelectric
means which gives a signal to the operator if the supply of tape is
low.
21. An apparatus as in claim 1 wherein said control means includes
automatic switches for sensing the presence of a rack, sensing the
condition of the tip being raised, sensing the presence of tubes on
both rows on said rack tray, and sensing when said rack is through
being porcessed.
22. An apparatus as in claim 21 wherein said control means
additionally includes means for providing an ejection pulse, an
ejection apparatus, said ejection pulse operating to allow said
ejection apparatus to eject a processed rack onto said surface
means.
23. An apparatus as in claim 1 wherein said control means has
circuitry providing for an automatic mode wherein said tubes are
sequentially processed and the rack advanced incrementally.
24. An apparatus as in claim 23 wherein said control means
additionally includes a manual mode circuit by which an operator
can perform one sampling and dispensing apparatus at a time.
25. An apparatus as in claim 24 wherein said control means also
includes an advance mode circuit and means to advance continually
the rack without allowing the pipette and tip means to process any
of the tubes contained therein.
26. An apparatus as in claim 25 wherein said control means
additionally includes a pipette mode circuit means adapted to allow
an operator to operate the pipette without operating the tip or
liquid sampling and dispensing means.
27. An apparatus as in claim 26 wherein said control means has an
exclusionary circuit means allowing only one of said modes to be
operated at a single time.
28. An apparatus as in claim 1 wherein said control means includes
circuit means adapted to prevent processing or operation of the
apparatus before the rack is in a correct position, the pipette is
in correct pumping sequence, before and when there is no
malfunction signal present.
29. An apparatus as in claim 1 wherein said control mans includes
an advance circuit means which will advance the rack until tubes
are sensed by said control, means, this allowing for a partially
filled rack to be processed.
30. An apparatus as in claim 1 wherein said control means includes
a binary shift register and solid state controls, the shift
register acting to automatically operate the control means to
provide a predetermined sequence of operation with appropriate
times and time delays therebetween.
31. An apparatus as in claim 30 wherein said control mans includes
a START switch means which is adapted, upon being held in depressed
condition, to provide automatic processing and stepping of said
shift register.
32. An apparatus as in claim 1 wherein said tip raising and
lowering means includes a tip motor means, the length of time of
rotation of said motor in either direction controlling the depth of
stroke of said tip means, said control means providing that
rotation of said motor means in one direction allows said tip to
move downwardly in both front and rear positions and rotation of
said motor in an opposite direction allows said tip to move
upwardly in both front and rear positions.
33. An apparatus as in claim 32 wherein said control means has
manually adjustable timer means to control the amount of rotation
of said motor means in either direction, thereby determining the
depth of stroke of said tip means in both said positions.
34. An apparatus as in claim 32 wherein said control means includes
a circuit means adapted to first reverse said motor means, provide
a predetermined delay period to allow the dynamic braking of said
motor to take effect, switch the contacts on said motor, provide a
second time delay of a predetermined amount of time and finally to
start said motor rotating in an opposite direction.
35. An apparatus as in claim 1 wherein said control means has an
external select switch adapted to allow an operator to control the
operation of the transporter externally, said control means also
having an external potentiometer means, said control means
including two timing means, said first timing means adapted to
provide a delay to allow the reagent to mix with a sample after it
has been dispensed above the level of the sample in the tube and
said second timing means adapted to provide a pulse to said tip
raising and lowering means to move the tip down below the level of
said mixed reagent and sample in the same tube, said potentiometer
providing adjustment ot said timer whereby an operator may select
the depth of additional stroke which the tip moves down into said
tube.
36. An apparatus as in claim 1 wherein said liquid sampling and
dispensing means includes a control circuit, said control circuit,
when activated, providing power to said control means for the
entire apparatus.
37. An apparatus as in claim 1 wherein said control means includes
circuit means to operate an auxiliary pipette or liquid sampling
and dispensing means which can be used in conjunction with said
liquid sampling and dispensing means.
38. An apparatus as in claim 37 wherein an auxiliary pipette is
provided on said liquid sampling and dispensing means and is
attached thereto in fixed position so as to be in a position to
dispense into the tubes in one row on said rack.
39. In an apparatus for transferring liquids with respect to
containers, especially test tubes, comprising a base, means for
incrementally moving a holder or rack having a plurality of
receptacles for the containers longitudinally along a path over the
base from an initial station to a final station, a movable nozzle
or "tip" above an intermediate station along the path,
positive-displacement pump means comprising a piston reciprocable
in a cylinder having a port for passage of liquid, a flexible
conduit connecting the port with the tip for communication
therewith, means for moving the tip relative to the base, and
control means for coordinating the operation of the several
means,
the improvement wherein:
the rack has two rows of receptacles for the containers extending
longitudinally of the rack,
the means for moving the tip comprises (1) means for moving it back
and forth transversely of the rows from a position over a
receptacle in one row of the rack to a position over a receptacle
in another row of the rack and (2) means for lowering and raising
the tip in each of its positions, and
the control means is constructed and arranged to perform one or
more cycles, each cycle including the following steps in the order
recited:
the rack is advanced in its path to bring a receptacle in one of
the rows under the tip, at which time the
rack-advancement-ceases,
the tip is moved down into the container under the tip,
the piston is moved through one of its alternate strokes while the
tip is in the container,
the tip is raised out of the container,
the tip is moved transversely of the rack to a position over a
receptacle in the other row,
the tip is lowered into the container under the tip,
the piston is moved through the other of its alternate strokes,
the tip is raised out of the container, and
the tip is moved transversely back to a position over the first
row.
40. In an apparatus for transferring liquids with respect to
containers, especially test tubes, comprising a base, means for
intermittently moving a test tube holder or rack longitudinally
along a path over the base from an initial station to a final
station, a movable aspirating and dispensing tip above an
intermediate station along the path, positive displacement pump
means comprising a piston reciprocable in a cylinder having a port
for inlet and outlet of liquid, a flexible conduit conecting the
port with the tip, means for lowering and raising the tip relative
to the base, and control means for coordinating the operation of
the several means, the improvement wherein: the apparatus also
comprises means for wiping the tip comprising a rotatable reel
carrying a wound supply of an absorbent wiping material in the form
of a tape of indefinite length, a driven take-up reel for the tape,
means for guiding the tape from the supply reel to the take-up reel
along a path adjacent the path of the tip, and means for pressing
the tape into wiping engagement with the tip whenever the latter
rises.
41. In an apparatus for transferring liquid with respect to
containers, especially test tubes, comprising a nozzle, a plurality
of receptacles, pump means, a flexible conduit connecting the pump
means to the nozzle for communication therebetween, means for
moving, relative to one another, the nozzle and the receptacles
including means for raising and lowering the nozzle in a path
relative to the receptacles, the improvement wherein there is
provided nozzle-wiping means comprising a rotatable reel carrying a
wound supply of absorbent wiping material in the form of a tape of
indefinite length, a driven take-up reel for the tape, means for
guiding the tape from the supply reel to the take-up reel along a
path adjacent the path of the nozzle, and means for pressing the
tape into wiping engagment with the nozzle.
42. Apparatus according to claim 41 comprising means for actuating
the pressing means in response to upward movement of the
nozzle.
43. In an apparatus for transferring liquids with respect to
containers, especially test tubes, comprising a rack having a
plurality of receptacles for supporting containers therein, a
nozzle, means for intermittently moving the rack to position the
receptacles successively under the nozzle, pump means, and a
flexible conduit connected to the pump means and the nozzle to
provide fluid intercommunication therebetween, an improved nozzle
module unit comprising a support, stationary, vertically extending
guide rods secured to the support, a cross-head slidably mounted
for motion up and down on the guide rods, a rotatable crank arm
having a roller secured thereto and projecting into a guideway in
the cross-head, means for rotating the crank arm, and means secured
to the cross-head for supporting the nozzle to move it up and down
with the cross-head.
44. Apparatus according to claim 42 in which the nozzle module unit
also comprises a pair of guide bars inclined to both vertical and
horizontal and the nozzle-supporting means is a holder slidable up
and down the guide bars.
45. Apparatus according to claim 52 in which the nozzle module unit
also comprises a pair of normally spaced-apart sponge rubber pads
and means for moving the pads towards each other and gainst the
nozzle as it moves upwardly.
46. Apparatus according to claim 42 in which the nozzle module unit
also comprises a pair of guide bars inclined to both vertical and
horizontal and the nozzle-supporting means is a holder slidable up
and down the guide bars, and the module unit also comprises a pair
of sponge rubber pads, mounted on rods slidable transversely with
respect to the lower ends of the inclined guide bars, spring means
for normally holding the pads in an opposed but spaced relationship
with each other, and means for urging the pads together against the
nozzle when the nozzle moves upwardly.
47. Apparatus according to claim 46 which also comprises means for
shifting the inclined guide bars back and forth between two
positions so that lowering of the nozzle holder lowers the nozzle
into different containers supported in the receptacles of the rack
under the two positions and the means for urging the pads together
is effective to press them against the nozzle in both
positions.
48. Apparatus according to claim 47 in which the means for urging
the pads against the nozzle comprises a pair of arms pivotally
mounted on parallel horizontal axes and rotatable cam means for
swinging the arms on their pivotal axes.
Description
DESCRIPTION OF THE INVENTION
This invention is directed to an automatically controlled
transporter apparatus for sequentially performing aspirating and/or
dispensing functions on a plurality of test tube pairs containing
liquid samples and reagents. It entails drawing off, through a
conduit, a volumetrically metered, freely adjustable, quantity of
liquid and for performing further functions on this metered
quantity through another conduit.
A major problem in the past in analytical testing of large numbers
of sample liquids, such as blood, has been the large amount of time
consumed in individually processing each sample-containing tube.
For instance, where a tube having a sample liquid therein has to
have reagent added thereto in order for certain tests to be
performed and analytical conclusions drawn, there are the problems
of adding exactly the right amount of reagent, handling the sample
tube so as not to spill or splash any sample liquid and the placing
of the sample tube in upright position in a rack. In addition, the
time consumed by such individual handling results in a serious time
delay in hospitals and private analytical laboratories in getting
the analysis results back to the physicians or, in the case of
non-medical testing, the party requesting the analysis.
Thee same problems occur in performing other types of laboratory
analysis. While metering and dispensing apparatus to perform this
function are known, they are mainly used in test laboratories,
e.g., biochemical and clinical laboratories, and are intended
either to deliver a predetermined volume of a liquid to be analyzed
(i.e., operating as a metering apparatus), or to deliver
successively several metered quantities, all of identical volume,
of the same liquid (i.e., operating as a dispensing apparatus), or
else to deliver simultaneously both a volumetrically predetermined
metered quantity of a liquid to be analyzed and a metered quantity,
also volumetrically predetermined but not necessarily of same
volume as the liquid to be analyzed, of a diluting liquid or of a
reagent (i.e., operating as a diluting apparatus). The use of these
apparatuses is becoming widespread as the demand for large volume
testing increases. However, the known apparatuses are often of low
accuracy, inasmuch as the volumetric quantities they meter lack
accuracy and are not readily reproducible, particularly when these
volumetric quantities are small.
This defect in the known devices is largely due to the fact that,
in these known forms of apparatus, adjustment of the metered
quantity is not "digitalized." I.e., adjustment is continuous and
such adjustment is often affected by errors in the reading of
scales, these being human errors attributable to distraction,
inattention or tiredness on the part of the operators. Moreover,
the known forms of metering apparatus operate in a rather brutal
way. First, an abrupt suction action occurs, then an abrupt
stoppage of the suction action and finally an abrupt discharge of
the metered quantity of liquid that has been sucked in. This often
results in cavitation or breaks in the columns of liquid (hydraulic
hammering effects) or in the formation of drops, both being a
source of material errors also occurring in manual handling of the
samples. The only way known to reduce the extent of the errors with
these known forms of metering and dispensing apparatus is by having
them handle relatively large quantities of liquid. This, of course,
is a major drawback when it is required to carry out a large number
of different tests on a liquid from one source since, in order to
do this, a corresponding number of samples is usually required of
the liquid and this means that quite a sizable starting quantity
will be needed. The only way in which this need for sizable
starting quantity of the liquid can be avoided is to reduce to the
greatest possible extent the volume of each sample.
Another problem in analysis has been the handling, of the sample
after it has been added to a reagent and before a separate function
is to be performed. I.e., the devices available have not been
sophisticated or flexible enough to coordinate several analytical
steps on the sample or sample and reagent.
Therefore, it is desirable to minimize all possible causes for
inaccuracy in metering. This is particularly important in
haematology where it is often desired to carry out a large number
of tests without having to draw off large quantities of blood from
the patients. One of the most relevant examples is in pediatric
haemotology.
According to the present invention, there is provided a solid state
controlled apparatus which includes an advancement mechanism which
moves the racks under a movable tip or nozzle which is actuated by
a grooved cam arrangement which lowers it into first one tube and
then the adjacent tube. After aspirating or dispensing the tip is
withdrawn and simultaneously wiped clean by a wiper mechanism
controlled by the tip. An ejector mechanism which ejects the
processed racks is also part of the apparatus.
The apparatus includes a pipette unit having a pair of pumps, each
with a suction and forcing piston, pumpable during a suction stroke
to draw off a predetermined volume and a switching valve associated
with said pump and able cyclically to pass from one condition in
which it causes said pump to communicate with one conduit to a
second condition in which it causes the pump to communicate with
another conduit. The pipette unit also includes a drive mechanism
able to cause said valve to pass from one condition to the other
and able cyclically to actuate said pump by imparting to the
reciprocating movement of the piston a substantially sinusoidal
action, said mechanism being adapted so that the suction strokes of
the pump occur when the valve is in said one condition and that the
forcing strokes of the pump occur when the valve is in said other
condition and so that each suction and forcing stroke may be
followed by an idling pause during which the valve passes from one
condition to another. Also featured is an adjustment means for
selectively adjusting the volume of said metered quantity of
varying the length of the stroke of the pump piston and display
means for displaying to the outside a number indicative of said
volume.
The apparatus also has a novel cam and toggle arrangement for
controlling the movement of the tip in a vertical direction in and
out of adjacent tubes in a test tube rack. The distance the tip
moves into each tube is controlled by the duration of the drive
motor for the tip. A second and/or third fixed tips may also be
used in conjunction with a movable tip to discharge reagents into
the back tube in a test tube rack. Also, an auxiliary pipette unit
may also be used in conjunction with the pipette unit furnished
with the transporter. The auxiliary pipette discharges through one
or two fixed tips. The apparatus is designed to be connected to a
flame photometer or other auxiliary equipment for direct analysis
of the diluted or reacted sample in the rear test tube.
A novel wiper mechanism is provided for wiping the tip as it moves
upwardly out of the tube after having either dispensed or aspirated
within the tube. A pair of reels, one a supply reel for a wiping
tape and the other a take-up reel, are mounted on a slidable tray
or drawer which may be pulled out from the apparatus to replace the
tape supply and/or take-up reel.
Accordingly, it is an object of this invention to provide an
improved sample moving and testing apparatus for use in analyzing
sample liquids carried by test tubes mounted in a rack.
It is a further object of this invention to provide an improved
dispensing and aspirating tip advance and retraction mechanism.
It is a further object of this invention to provide a novel test
tube transporter and sample aspirator and dispensing mechanism for
use in conjunction with various tests run on the samples contained
in the test tubes.
It is a still further object of this invention to provide an
improved tip wiper mechanism for use in a test tube transporter and
sample dispensing apparatus.
Another object of this invention is to provide an automatic test
tube transporter and sample dispenser apparatus having a novel
solid state control system which minimizes operator involvement and
maximizes flexibility of the apparatus in performing various
analytical functions upon samples carried in test tubes.
Another object of the invention is to provide a novel test tube
transporter and sample dispenser apparatus which may operate
continuously upon a plurality of test tube samples in parallel rows
within a single test tube rack or in a pair of test tube racks or
may be manually operated upon each tube or pair of tubes.
It is a further object of this invention to provide a novel test
tube sample transporter and dispensing apparatus which may be used
in conjunction with auxiliary pipettes or external control
units.
It is a still further object of this invention to provide a novel
test tube transporter and sample dispenser apparatus having a solid
state control system which includes a binary shift register.
These and other objects of this invention will become apparent when
reference is had to the following specification and the
accompanying drawings in which:
FIG. 1 is a perspective view of the transporter showing the working
surface, the control and pipette modules and the movable tip
module;
FIG. 2 is a side view of the tip module, partially in section,
showing the various interrelationships between the tip moving
mechanism and the tip wiper mechanism;
FIGS. 3 and 4 are front views of the tip module with the case
removed, showing the tip guide mechanism when the tip is in
retracted and down positions, respectively;
FIG. 5 is an exploded view of the cam and cam clutch driving
assembly for the tip wiper mechanism;
FIG. 6 is a partial front view of the wiper cam assembly showing
the interrelationship between the cam, the cam clutch, the wiper
toggle mechanism and the follower;
FIG. 7 is a top view with parts broken away and in section of the
tip module shown in FIG. 2;
FIG. 8 is a perspective view of the apparatus similar to FIG. 1,
but showing the wiper tray pulled forward and with the pipette and
electronic control modules removed and the casing off the tip
module;
FIG. 9 is a front view of the supply reel subassembly showing the
supply spindle and take-up spindle;
FIG. 10a is a two-dimensional plot of the grooves in the
cylindrical tip cam;
FIG. 10b is a cross-section taken along lines 10b--10b of FIG.
10c;
FIG. 10c is a side view of the cylindrical tip cam;
FIG. 11 is a partial planar view showing the teeth in the take-up
spindle shown in FIG. 9;
FIG. 12 is a plan view of the wiper tray showing the interrelation
of the supply and take-up reels with the supply and take-up
spindles;
FIG. 13 is a side view with parts in section of the transmission
means for the wiper tape spool;
FIG. 14 is a plan view of the device shown in FIG. 13;
FIG. 15 is a schematic view of the A.C. motor driver circuitry;
FIG. 16 is a schematic view of the driver and breaking circuit
typically used in FIG. 15;
FIG. 17 is a schematic view of the pipette logic circuitry;
FIG. 18a is a partial schematic view of the tip motor logic
circuitry;
FIG. 18b is a schematic view of the remaining tip motor logic
circuitry and has matching leads with FIG. 18a;
FIG. 19 is a schematic view of the shift register and logic
circuitry;
FIG. 20 is a schematic view of the advance and start logic
circuitry;
FIG. 21 is a schematic view of the lamp driver circuitry;
FIG. 22 is a schematic view of a typical lamp driver circuit used
in FIG. 21;
FIG. 23 is a schematic view of the manual switch buffers and logic
circuitry;
FIG. 24 is a schematic view of the power-up reset used in the
circuitry shown in FIG. 23;
FIG. 25 is a schematic view of the automatic switch buffers and
logic circuitry;
FIG. 26 is a schematic view of the malfunction and alarm logic
circuitry;
FIG. 27 is a schematic view of the interface circuit used in FIG.
26;
FIG. 28 is a schematic view of a typical timer circuit used in the
circuitry shown in FIG. 26;
FIG. 29 is a schematic view of the relay driver circuitry used in
FIG. 17;
FIG. 30 is a diagrammatic view of a sampling and dispensing
operation;
FIG. 31 is a diagrammatic view of a sampling and dispensing
operation using a double bore tip;
FIG. 32 is a cross-sectional view taken along lines 32--32 in FIG.
31;
FIG. 33 is a diagrammatic view showing an alternative sampling and
dispensing arrangement.
The apparatus is provided with three modules; the pipette,
electronic and tip modules. Controls on the pipette and electronic
modules enable an operator to adapt the apparatus to perform many
and various analytical operations and functions.
The general operating function of the transporter is determined by
positions of an auxiliary pipette switch and an external control
switch on the function control panel in the electronic modular
unit. Details of the operation may vary according to the operator
set-up; for instance, in the manifolding of pipettes. There are
four basic switch combinations; both OFF, either one ON, and both
ON.
In the basic operation function, i.e., with the auxiliary pipette
and external control switches OFF, the function control option
instituting operations in the following arrangements:
1. a sample in the front tube row diluted with a sample reagent
into the back tube row;
2. a reagent dispensed in each tube row; or
3. one or two reagents dispensed in the back tube row only.
With the auxiliary pipette switch ON and the external control
switch OFF, the function control option provides for the use of an
auxiliary dispensing pipette in conjunction with the apparatus.
Basically, this application entails the preparation of a sample in
the front tube row diluted with a first reagent from the
transporter pipette and further with second and third reagents from
the auxiliary pipette. The transporter pipette will be manifolded
in the conventional way through a single or double-bore tip moved
by the tip mechanism. The auxiliary pipette uses one or two fixed
tips mounted over the tubes in the rear row. Discharge from the
pipette tips is adapted to occur simultaneously. The auxiliary
pipette may use one or two pumps in the pipette module as required
by the particular application. The fixed position auxiliary tip
will not touch the wall of the test tube during the operation soc
that dispensed volumes should be relatively large to minimize
break-off reproducibility errors.
With the auxiliary pipette switch OFF and the external control ON,
the function control option provides for applications requiring
supply of diluted samples to a readout instrument. An interface
unit is then provided to adapt perspective readout instruments to
the apparatus. In this operation, the tip module is provided with a
twin tip in the tip mechanism. In the alternative, special
configurations may be provided for other readout instruments to
provide minimum sample carry-over and rapid sample feed to the
instrument. In this adaptation, the rear tubes in the rack are
omitted and a fixed cup is installed on the transporter. The
transporter has a single movable tip for sample and diluent.
With the auxiliary pipette switch ON and the external control
switch ON, the operation is similar to the one just described
except that it allows for the introduction of one or two additional
reagents through the fixed tips in the latter part of the
analytical sequence.
This device is an improvement over the apparatus shown and
disclosed in copending application Ser. No. 126,782, filed Mar. 22,
1971, entitled AUTOMATIC TEST TUBE TRANSPORTER AND SAMPLE
DISPENSER. The improvement over the apparatus described in the
copending application consists of the electronic solid state
controls and a re-designed tip mechanism. The new tip mechanism
facilitates many other analytical operations. The pipette unit used
in said copending application is identical with the pipette unit,
including the pumps and motion converting means used in this
application.
Since the pipette units used in both the apparatus of the copending
application and the pipette unit used in the instant device are
identical, the contents of said copending application are hereby
incorporated by reference in their entirety.
The structure and operation of the pipette module is described on
pages 22 to 36 of said copending application. The details,
including the drawings, are not included as a part of this case but
the unit is identical. Generally, the apparatus is a two-piston
sinusoidal stroke metering and dispensing device. A motor drives a
motion converting mechanism through a gear box. The motion
converting mechanism comprises a cylindrical housing having a
hollow cylindrical insert member adapted to be rotated therewithin.
Said member is driven by said gear box. There are two parallel
juxtapositioned longitudinal slots or slideways in the inner
surface of said tubular member. A second insert member is located
within said tubular member and locked against rotation therewithin.
The second insert member, however, is mounted for longitudinal
sliding within said tubular member and its position relative to
said first rotatable insert member is adjustable by means of a
screw adjustment shaft. The first rotatable insert member has a
pair of longitudinally extending slideways which carry one end of a
crank mechanism. The other end of the mechanism is fixed within a
jeweled bearing within said second nonrotatable insert member. The
crank mechanism includes a shaft connecting said insert members
with a bearing connected thereto. A slide block rides on the
outside of said tubular member and is connected to said bearing. As
the first insert member rotates, the crank and crank pin describe
an arc and the motion is transmitted to the bearing and slide block
on the tubular member housing. Thus, the slide block reciprocates
vertically as the first insert member rotates. The slide block is
connected to a crank bar, one end of which is fixed to said tubular
housing and the other end is fixed to the lower driving portion of
the pump piston located within said pipette unit. The motion
converting means thereby converts rotary motion into sinusoidal
reciprocal motion which is transmitted to the pump pistons at the
end of the crank bar.
The pumps which make up part of the pipette unit are positive
displacement type pumps having a cylinder, a constant diameter
piston, the driving end of which is connected to the stroke bar of
the motion converting means and a valve switching mechanism. There
are two port bores connected to each pump, there being two pumps,
and the valves are adapted either to switch from one port bore to
another between each stroke, whether positive or negative, of said
crank bar, or to remain in position. The valve itself has a
cylindrical member located within the cylinder housing of each pump
and has a V-shaped connecting channel which alternately connects
each port bore with the interior of said cylinder when the valve is
rotatably reciprocated. A priming means is provided on each pump.
The piston is easily removed from the pumps of varying volume.
The gear box is adapted to drive a valve switching mechanism which
converts the rotary motion of the gears to longitudinal
reciprocating motion. At the pumped end of the valve switching
mechanism are reciprocally rotatable driving pins. The driving pins
may be placed in one of two positions, the first position being a
driving position in which the pin is operatively connected to the
pump valve whereupon the valve will switch from one port to another
and the second being in a position in which the pin is disconnected
from the valve and the valve remains in a preselect position,
thereby maintaining only one port in connection with the cylinder.
The driving gears for the valve switching mechanism have relieved
areas thereon whereby after said valves are switched, no motion
takes place while the piston is stroked either negatively or
positively.
Thus, the pipette pumps can be operated in several fashions, the
first being where the valves switch in both pumps between pumping
strokes, the second being where neither valve changes ports during
the pumping strokes, the third where one valve remains in a
predetermined position and the second valve switches between piston
strokes. Also the pistons may be stroked in the same direction
simultaneously or may, through changing the relative gear
positions, be alternately stroked or be simultaneously stroked in
opposite directions. Thus, a great variety of dispensing and
sampling operations are possible with the pipette unit.
The pipette unit also has counters located thereon which are
operatively connected to the main threaded shafts for adjusting the
stroke of the pump pistons. These counters are used to indicate to
the operator the amount of stroke and consequently, the particular
volume of each pump during that operation.
The pipette unit has a RUN switch and a SET switch thereon which,
when both are depressed, provide power to the tip and wiper
mechanism. The operation of the pipette unit and its components
actuates several switches within the electronic solid state control
system. The RUN and SET swtiches are shown in FIG. 17, the pipette
logic circuitry diagram. Also shown in that diagram are switches
569, 570 and 571. They are, respectively, a pipette limit position
switch, a pipette left position limit switch, and a pipette limit
right position switch. Also in the drawing is switch 574 which is a
pipette limit set position switch. The operation of these contact
switches will be described later herein.
TIP MECHANISM
Referring now to FIG. 1, there is shown the transporter apparatus
generally designated as 1. It consists of a base unit 2 having a
supporting structure 3. A planar surface 4 is located on top of
base portion 2 and has an exit surface 5 separated therefrom by
base unit projection 6 and dividing bar 46. A pair of other base
portion projections 7 and 8 act together with border strips 9 and
10 to provide an alignment guidance for the test tube racks. Member
10 contains a slot 11 therein in which rides rack guide arm 12. Arm
12 is tapered as at 14 and has a relieved area as at 13 by which an
operator may grasp the arm to retract it when placing racks into
the transporter. The member 10 and arm 12 and its accompanying
drive mechanism form the contents of a separate application and is
not described in detail in this specification.
The racks such as R are placed on surface 4 and contain adjacent
rows of tubes such as T.sub.1 and T.sub.2. Usually, the sample to
be tested, whether it be blood, urine, or some other substance, is
placed in the row containing tube T.sub.1 and a reagent to be
reacted with said sample is placed in the row containing tube
T.sub.2.
A raised portion of the transporter, 15, houses a rack-advancement
mechanism, not shown in detail but generally similar to the
rack-advancement mechanism shown and described in said earlier
copending application. The rack-advancement mechanism has teeth,
such as 51, which project outwardly through a slot 50 is the
housing. The housing also contains a rack sense contact 47 which
tells the electronic logic of the transporter when a rack is in
place and is ready for processing. Also located alongside the path
of rack R in front of the housing is a tube sensing switch, such as
49, which projects through a slot 48 within housing 15, and 49'
projecting from the end of bar 46. The sensing switches 49 and 49'
are not shown in detail but may be simply a spring-loaded slide
switch or a microswitch. A rack-ejector mechanism is also located
within housing 15 but is not described in detail. Instead of using
flipper arms, as used in said earlier copending application, the
mechanism is adapted to drive push rods, 54 and 55, shown in
phantom, through cylindrical apertures 52 and 53 in said housing to
eject racks along the surface 5 once processing on the tubes
contained therein has been completed.
The main console containing cabinet 16 is supported on housing 15
and contains a pipette module unit identical with the one shown and
described in said earlier copending application. The unit contains
pumps 18 and 19 having windows 20 and 21 cut therein respectively.
The windows are for viewing the liquid within the cylinder
chambers, the chambers preferably being made of glass or clear
plastic. The pumps have ports, such as 20' and 21', located thereon
and are used as previously described in this application and
described in detail in said earlier copending application. The
pistons (not shown) contained within the pumps are connected to
stroke bars such as 8' and 9' which, in turn, are connected to
motion converting means within the pipette module.
A SET switch 26 and a RUN switch 27 are located on said pipette
control panel and are used to set both the pipette module and
electronic control module into operation. Control knobs 25 and 26
are used by the operator to set the stroke adjustment or, in other
words, the length of said stroke which each pump will incur.
Counters 22' and 23' are adjacent the control knobs to indicate to
the operator the present setting of the stroke length. Thus, the
operator can quickly determine the volumetric capacity of each pump
during the processing of the samples.
Located adjacent the pipette module is the tip housing module 28.
It consists of a frame 29 having a window 30 therein through which
the tip may be viewed or, in the alternative, the panel 30 may be
non-transparent.
Located on the other side of the tip module is the electronic
control module 32. It has an upper control face 31 which contains
the major mode switches. It contains automatic mode switch 39,
manual mode switch 40, advance mode switch 41, pipette mode switch
42, reset switch 43 and start switch 44. These switches are shown
designated by different numerals on FIG. 23, the manual switch
buffer and logic circuitry. Located directly above said switches is
a lamp display console 45 which includes the following lamps to
indicate the following conditions: open tray; advance jam; tip
wiper; eject; pump sequence; tip down front; pipette; tip down
back; tip up; advance; automatic; manual; advance and pipette.
Located underneath said major control switches on panel 33 are the
auxiliary pipette switch 34 and the external control switch 35.
Adjacent said switches on panel 36 are front stroke adjustment knob
38 and back stroke adjustment knob 37. The latter two knobs control
the depth to which the transporter tip moves into tubes such as
T.sub.1 and T.sub.2.
All three module units are supported on a support member 16'.
Located on the underside (not shown) of member 16' is a fixed
auxiliary pipette tip member 30'. As previously stated, the tip
module unit and the electronic control unit will be described in
detail whereas the pipette module unit, being identical with that
disclosed in said earlier copending application will not be
described nor shown in detail.
Referring now to FIG. 2, there is shown in section, the tip module
unit 60 the major components of which are tip holder, guide
mechanism, and guide driving means. During the description of the
structure and operation of this portion of the apparatus, reference
may be had to FIGS. 3, 4, 5, 6, 7, 8, 10a, 10b, and 10c.
A front mounting casting 61 supports most of the major components
of the mechanism. It is connected, in any suitable fashion, to a
base plate member 62 which, in turn, is connected to a rear plate
63 to form the supporting structure for the entire mechanism.
Casting 61 has a plurality of projections 64, 65, and 66 on the
rear face thereof. It also has a pair of bores 67 therein each of
which receives a bushing 129. Another pair of bores 68 are in the
top of said casting and each receives the forwardly extending
portion 99 of a tip guide rod. Projection 64 has a bore 69 located
therein with counterbored sections 71 and 72. Located within said
counterbored areas are bearings 70 and 73. The bearings support a
wiper cam shaft 75 therein. One end of said wiper cam shaft has a
gear 74 thereon having teeth 74' and a flanged portion 77. A nut 76
secures said gear to the end of said cam shaft 75.
Gear 74 is in operative drive action with a gear 86. Gear 86 has a
flange portion thereon and a machine screw 88 secures gear 86
through lock washer 89 to the drive shaft 85. Drive shaft 85 is
located within a bore 79 in projection 65. Bore 79 has counterbored
areas at the end thereof such as 80 and 81 in which are located
journal bearings 82 and 83 which support shaft 85 for rotation.
Mounted for rotation with gear 86 on its flange portion 87 is a
crank 90. Crank 90 is best seen in FIGS. 3 and 4.
A bore 91 in projection 66 has a pair of journal bearings 92 and 93
therein which support a cylinder cam shaft 94 for rotation therein.
On one end of cylinder cam shaft 94 is a washer 98 and a splined
section 94'. Splined section 94' carries cylindrical cam 216
thereon for rotation therewith. The opposite end of shaft 94 is
threaded as at 96 which receives a nut 97 and washer 95 to lock the
shaft in projection 66 against longitudinal movement therein.
The upper end of crank 90 has a circular cam follower 118 mounted
thereon by spacer 119 in machine screw 120. Follower 118 is adapted
to ride in a longitudinal slot 117 in a cam follower guide member
113'. Cam follower guide member has projecting portions 115 and 116
which together form slot 117. The opposite ends of member 113' are
vertically bored as at 114' in FIG. 2 so as to receive a pair of
guide rods 111 and 111'. The tops of guide rods 111 and 111' are
secured by locking washers or members 112'. They are secured to
projecting tab portions such as 110'. Tab portions 110' are
extensions of front castings 61.
The lower end of crank 90 (as seen in FIG. 2) has a top dead-center
cam 123 secured thereto by machine screw 122. The configuration of
cam 123 can be seen in FIGS. 3 and 4. Cam 123 has rounded surfaces
such as 124 which are adapted to engage the actuator arm 126 of a
microswitch 125. Microswitch 125 is secured to the device in any
suitable manner, such as by pins 127.
The guide rods 111 and 111' support a cross slide (or cross-head)
having opposed sections 113 and 113' for sliding movement thereon.
As seen in FIG. 3, the cross slides have projecting transverse
portions 115 and 116 connecting the two together. The guide bars
99' and 99" project outwardly from the top of casting 61 and are
angled downwardly. They support a tip holder 100 for up and down
sliding movement thereon. Tip holder 100 has a pair of bores, such
as 101, as shown in FIG. 2 which receive the guide bars. Tip holder
100 has an aperture (not shown) for receiving the tip T, as shown
in FIG. 3. A latch member 104 is supported on a shaft 102 and is
biased inwardly by spring 103 to hold the tip T in position. The
tip holder also has bores 106 and 106' for receiving a pair of
stabilizing rods 105 and 105'. Stabilizing rods are received, at
their end portions, in projection member 116 of the cross slide. As
shown in FIG. 3, the tip T has a reduced diameter portion T' which
is the maximum portion which is wiped.
The ends of guide bars 99' and 99" are received in a cam block 254
as shown in FIG. 7. Cam block 254 has a pair of tab portions, such
as 255, which are split as at 256 to receive a machine screw 257
for locking the cam block 254 in position on bars 99' and 99". The
upper portion of casting 61 is cut away as at 258 to accommodate
longitudinal movement of cam block 254. Located within cam block
254 is a "toggle" mechanism comprising a snap-action lever 266 and
a pair of guide cylinders 259 and 260. Located within member 254
between the cylinders is a fixed cylinder 268 having a transverse
bore receiving for rotative movement therein a pin member 267 which
is secured to lever 266. The upper portion of cylinder 268 is
slotted as at 272 to allow lever 266 to reciprocally rotate
therein. Located within cylinder 268 is a compression spring 269
which urges a ball 270 upwardly against a downwardly directed
pointed projection or tab 271 on lever 266 which causes the lever
266 to snap from one position to the other when the lever 266 is
moved clockwise from the position shown in FIG. 2 till the point of
271 passes over to the other side of ball 270. Since both cylinders
259 and 260 are identical, only one will be described. Guide
cylinder 259 has a plunger 262 located therein with the upper
portion cut away as at 261. The upper portion of 262 supports a pin
265 which is received within a notch on the end of lever 266. The
other end of lever 266 has an identical notch. A cam follower 263
is secured to plunger 262 by means of a machine screw 264. Cam
follower 263 is adapted to ride in grooves such as 240 in
cylindrical cam 216. Cylindrical cam 216 has the hub portion 218
which extends inwardly therein to cover the splined portion 94' of
shaft 94. The interior of cam 216 is cut away as at 217 to
accommodate projection 66 on casting 61.
Referring now to FIGS. 10a, 10b, and 10c, the cam 216 will be
described. FIG. 10a shows a development of the grooves within the
cam. In other words, FIG. 10a is representative of the
circumference of cam 216 in a two-dimensional plane. The cam
contains two grooves, namely groove 246 and groove 248, which have
side walls 240 and 247, respectively. The grooves are essentially
linear and have rounded terminal portions 241 and 250,
respectively. Groove 248 has a curved wall 242 at the opposite end
thereof and has a portion which tapers upwardly as at 243 so as to
provide a more shallow groove. The tapered portion 243 terminates
at 244. Groove 248 has a curved wall section 251 which forms a
portion of an upwardly tapered groove portion 252 which terminates
at 253. The cam is shown in side view in FIG. 10c and the view
shows that groove 240 is initially deep and then rises as at 243 to
planar section 244. FIG. 10b shows a cross-sectional view of the
cam taken along lines 10b-10b of FIG. 10c and shows the general
cross-sectional configuration of the grooves at the tapered or
upwardly slanted groove portions. As shown in FIG. 10c, the cam has
a gear 249 mounted on one end portion thereof, In the alternative,
referring back to FIG. 2, a gear 220 may be provided on the end of
splined shaft 94' which fits within a bore 219 in cam 216. In
either construction, the gear is adapted to mesh with a gear 221
which is operatively connected to drive shaft 85. Gear 221 has a
flange portion 222 with a slot 223 therein. An extension of drive
shaft 85 contains a roll pin 224 which engages within slot 223 to
lock the gear and drive shaft against relative rotation. Gear 221
is connected to a coupling member 225 which in turn is connected to
the main drive shaft 226 which projects through an aperture 227 in
rear plate 63.
A machine screw 231 mounts a camming plate 230 to rear plate 63.
Cam plate 230, although not shown in front view configuration, is a
generally U-shaped member having two extending arm portions such as
229. Mounted on gear 221 for rotation therewith is an actuator bar
228. As gear 221 rotates, actuator bar 228 describes an arc which
eventually forces it to abut one of the depending portions, such as
229, of member 230. Such an action forces member 230 to rotate on
screw 231 and, as shown in FIG. 7, member 230 has a camming surface
on one upper corner thereof such as 236. The camming surface 236 is
adapted to engage a microswitch roller 235 which is supported
between arms such as 234 of a switch actuator 233. The switch
actuator 233 extends from a microswitch 232 and when actuated gives
enabling signals to the solid state control logic.
Referring again to FIG. 7, the member 230 is shown to have a flange
portion 236 with a semicircular recess 239' therein. Rear plate 63
has a bore therein which supports an insert member 237. Insert
member 237 has a bore therein which supports a helical compression
spring 237'. Spring 237' biases a ball 238 into engagement with the
aperture 239' in the rear of member 230. The compression spring and
ball maintain member 230 in actuated position until the actuator
bar 228 rotates around in a reverse direction and engages the other
leg from the one previously engaged. In other words, this prevents
the member 230 from assuming normal position before the actuator
bar has again made contact. Thus, it is seen that the member 230
has two positions.
Referring again to FIG. 2, the wiper cam shaft 75 is connected via
an extension 78 to an extension portion 170. Mounted on shaft
extension portion 170 is the cam and cam clutch assembly for
operating the wiping mechanism.
TIP WIPER ASSEMBLY
Referring now to FIG. 8, there is shown the tip wiper bars and the
wiping tape assembly tray. The wiping assembly is controlled by the
cam and cam clutch wiping assembly 140. Assembly 140 is shown in
detail in FIGS. 5 and 6. It consists of a cam member 145 having a
large-diameter peripheral camming surface 141 and a small-diameter
camming surface 146. The cam surfaces are merged smoothly as at 147
and 148. The cam is preferably made of nylon and has a projecting
cam portion 151 having a rounded surface at one end thereof the
center of which is recessed as at 152. Extending downwardly away
from portion 151 is portion 156. Portion 156 is a camming surface
having a recessed center area 157 and a main body section 155. The
cam 145 itself is relieved as at 149. A bore 154 is provided for
locking onto the cam shaft and a keyway 153 is provided to lock the
cam against rotation relative to the cam shaft. The angle X is
one-half of the distance of camming surface 146 and is preferably
about 770.degree.. Member 142 and member 144 are clutch washers
having square apertures 158 and 166, respectively, therein.
Received between the cam washers is the cam clutch plate 143. The
clutch plate 143 is generally circular having a reduced-thickness
portion 159 and a central bore 160' therein. A pair of ears 161 and
162 are located 180.degree. apart on the cam plate. A notch 165' is
located approximately midway between the ears and a second notch
consisting of deep notches 163 and 164 and projecting tab portion
165 is located 180' opposite from notch 165'. Angle Y, shown in
FIG. 5, is preferably approximately 38'.
Referring now to FIG. 6, the cam assembly is shown assembled
together with its "toggle" or snap-action mechanism and wiper bars.
The Snap-action assembly for the wiper mechanism is shown
designated generally as 180. The mechanism is shown in side view in
FIG. 2. Snap-action assembly 180 has a pair of guide cylinders 207
and 184. Since both the guide cylinders are identical, only one
will be described in detail. Cylinder 184 is maintained in place by
retaining rings 188 and 184'. Mounted for vertical sliding movement
within cylinder 184 is insert member 187. Insert member 187 has a
slot 189 and a pin 190 extends transversely through the slot into
the adjacent portions of 187. Member 187 is relieved as at 191 to
receive a helical compression spring 192. A vertical slot 195 is
cut in the wall of cylinder 187 and a plunger 193, having a pin
194, is adapted to ride within aperture 191 of member 187. Pin 194
rides within slot 195 and defines the limit of travel of plunger
193. Cylinder 184 is mounted in an aperture 181 of base plate
62.
Mounted between cylinders 184 and 207 is a cylinder 196.
Maintaining rings, such as 196', maintain 196 in place on plate
member 62. Cylinder 196 has an aperture 197 therein which receives
a helical spring 198 which, in turn, biases a ball bearing member
199 upwardly. Cylinder 196 is relieved at its upper extremity to
define a notch having a pin 201 extending thereacross into the
adjacent walls or prongs of 196. A snap-section lever 202 is
mounted for reciprocal pivotal movement on pin 201. A pointed
projecting tab 209 is adapted to engage either side of ball 199 and
thus produce a snap-action as lever 202 is reciprocated. The ends
of lever 202 have notches such as 203 which engage pins 190 mounted
on the relieved areas of the insert members 187. A pair of tab
portions such as 204 project upwardly from the top surface of lever
202 and have pins such as 206 to secure rollers 205 and 208 in
place. Rollers 205 and 208 are adapted to engage camming portion
156 of cam 145.
Plunger pin 193 is adapted to ride on a twist disc 377 and engage
in a slot 380 thereon. The operation of the twist disc will be
described later herein.
Referring now to FIG. 2, there is shown beneath cam assembly 140 a
cam follower barrel 210. The barrel is preferably made of plastic
and has an aperture 212 therein. Aperture 212 receives a helical
spring and a follower member 214. Follower member 214 has two
longitudinally spaced and parallel extending arms 215 and 216. Arm
216 is adapted to ride in a notched area 214 of follower barrel
210. Member 215 is adapted to ride on the lower surface of tab
portion 165 of clutch plate 143. At either limit of its travel, it
snaps into notches 163 or 164, depending on the direction of
rotation.
In projection 65 of casting 61 is a plate follower 167. Plate
follower 167 is engaged by the top of camming portion 151 and has a
stud 171 adapted to engage in notch 165' of clutch plate 143. A pin
168 (FIG. 6) extends from the opposite side of plate follower 167
into an aperture 169 in projection 65. A helical spring 170
maintains the plate follower in a downwardly biased condition,
thereby allowing the stud 171 to snap into notch 165'.
Ears 161 and 162 on clutch plate 143 are adapted to engage a pair
of rollers 177 and 177' which control the operation of the wipers.
Referring to FIG. 6, roller 177' is shown attached by pin 176' to
wiper crank 172'. Wiper crank 172' has an aperture 173' therein
which receives one end of wiper bar 129'. The end of wiper crank
172' is split to provide grapsing portions such as 174' and has
apertures therein to allow for a machine screw 175' and nut 176' to
securely fasten the wiper crank to the wiper bar.
Wiper bars 131 and 131' are shown extending from the main portion
of the apparatus outwardly in FIGS. 2 and 7. They have a bent
portion such as 130' and a flattened end potrion such as 132 and
132'. The end of each guide rod 99' and 99" has a pair of holes
bored therein, on parallel axes. The holes receive rods 133 and 134
which are attached at one end to support pads 136 and 136'. Mounted
on support pads 136 and 136' are wiper pads 137 and 137' preferably
made of sponge rubber or like material. FIG. 7 shows the plan view
configuration of the wiper pads. A helical spring, such as 135, is
mounted on rod 133 and a flange on the end of rod 133 keeps the
spring 135 in place. The flattened portions 132 and 132' of the
wiper bars are adapted to engage the ends of rods 133 and 133' and
force them inwardly against the action of compression springs 135
and 135'. The wipers are adapted to wipe the tip as it is withdrawn
upwardly through slot 367 in wiper tray 352, shown in FIG. 12.
Wiper tray 352 is shown in FIG. 12 and consists of a flattened
planar portion 354 cut away as at 368 and provided with a border
strip 353. As shown in FIG. 8, the wiper tray is supported within a
bracket member 301 having top surfaces 302 and 303. A plurality of
mounting holes such as 302' provide for mounting the pipette and
solid state control logic modules on portions 302 and 303. Bracket
member 301 has a depending portion 304 which allows for operation
of the wiper bars 131. The wiper tray 352 is attached to the
brackets by slide blocks 355 and 355'. The slide blocks are mounted
to the bottom sides of portions 302 and 303 by suitable fastening
means, such as 356. Rod anchor blocks, such as 358, 358', 359 and
359' support tray guide rods 357 and 357' by which are received
slide blocks 355 and 355'. Therefore the wiper tray is mounted for
sliding movement out of bracket 301. A pair of biased snap
fasteners (not shown) are used to lock the wiper tray within
bracket member 301 against inadvertent displacement. The pull
exercised by an operator of the apparatus is sufficient to overcome
these snap locks.
The wiper tray, as shown in FIG. 12, is seen to have a take-up reel
361 mounted for rotation about pin 361' and a supply reel 360
mounted for rotation about pin 360'. The wiper tape W is trained
around pulley 362 mounted for rotation on 364 and then around wiper
spool 366 and around pullyey 363 mounted for rotation on pin 365
and eventually to the take-up reel 361. As shown in FIG. 12, the
tape travels in opposite directions on either side of slot 367
through which the tip T moves. The slot 367 is elongated to provide
for the tip moving up and down in two positions to enter test tubes
in both rows of a rack tray. Also shown in FIG. 12 is phantom, are
the positions of the wiper pads 137 and 137'.
Referring now to FIG. 9, there is shown the bracket 301 having
raised portions 302 and 303 and depressed portion 304. Depending
from portion 302 is bracket 305 which rotatably supports on pivot
pin 308 a torque arm 309. Screw 310 maintains the components in a
predetermined relationship. Flange portions 306 and 307 support
between them a roller (not shown) around which is wound one end of
a negator spring 310. A screw 311 maintains the roller and negator
spring in place. The negator spring is attached at its other end by
screw 311 to torque arm 309. Torque arm 309 carries a mounting 316
attached to which is a slip clutch 317. A machine screw 312 secures
an L-shaped bracket 313 to the bottom of torque arm 309. A clutch
spring anchor 314 is mounted on the bottom portion of bracket 313
BY MACHINE SCREW 315. Connected to slip clutch 317 and resting upon
clutch spring anchor 314 is a torquing spring 319, one end of which
is received in an aperture in pin member 318 which depends from
slip clutch 317. A shaft 320 extends upwardly and is mounted in any
suitable fashion to a flange 322 of supply spindle 321. Supply
spindle 321 has a groove around the periphery thereof which
receives a resilient or elastic band 323. Supply spindle 321 is
adapted to engage the tape which is wound on supply reel 360, as
shown in FIG. 12. Supply spindle 321 is biased inwardly to ride
against the supply of wiper tape W on reel 360 by negator spring
310.
An anchor block 324 depends from the underside of portion 303 and
mounts a torque arm 325 for rotation about a pivot pin 329. A
machine screw 330 maintains the components in assembled
relationship. A pair of flange members 326 and 327 support a roller
(not shown) therebetween, around which is wound a negator spring
328. A screw 331 maintains the roller and negator spring between
flanges 326 and 327. The other end of negator spring 328 is
fastened to the opposite end of the torque arm by screw 329.
Mounted on the lower portion of torque arm 325 is a motor bracket
332. Machine screws 333 are used to secure motor bracket 332 to the
torque arm. A motor 335 is secured to the lower portion of bracket
332, which is C-shaped in configuration, by machine screws 336. The
screws 336 secure the motor to the lower portion 334 of the
bracket. A shaft 337 extending from motor 335 is connected to a
flexible coupling 338 and a take-up clutch 339.
Shaft 340 is connected to a flange 342 of a take-up spindle 342.
The periphery of take-up spindle 342 has a raised scalloped center
portion 342'(FIG. 9) such as shown in FIG. 11. It consists of a
plurality of arcs which intersect in points such as 345. The arcs
are designated as 343. The outline of the remainder of the
circumference of spindle 342 is shown in phantom in FIG. 11 and
designated as 344. Thus, it is seen that the points 345 project
outwardly beyond the peripheray of the remainder of the spindle and
are adapted to puncture and grasp the wiper tape W on the take-up
reel.
The supply spindle 323 and the take-up spindle 342 are shown in
position in FIG. 12. Arm 346 is adapted to engage a microswitch 347
having an actuator arm 350 and contact roller 351. The microswitch
347 is secured to the base of bracket 301 by studs 348. It can be
seen from FIG. 12 that the spindles engage the wiper tape W on the
reels.
Referring now to FIGS. 2, 13 and 14, there is shown mechanism for
controlling the advancement of the wiper tape itself. The mechanism
comprises a twist disc 377 having a sloping cam surface 379 which
terminates in a peripheral slot 380. The cam surface 379 commences
at 381 and is approximately 180.degree. in arcuate length. The
twist disc 377 has a hub portion 378 which is adapted to rotate on
shaft 375. Plunger 193 of escape cylinder 194 is adapted to ride on
cam surface 379 until it drops down into peripheral slot 380. A
supporting frame 370 is mounted beneath the twist disc and has a
projecting portion 372 having a hollow passageway therein 372' and
bearings 374 and 373 mounted at either end of said passageway in
countersunk portions. A shaft 375 drives twist disc 377 which rides
on bearing 376. Frame 370 has supporting structure (shown in
phantom in FIG. 13), such as brackets 371, machine screws 369, etc.
On the opposite end of shaft 375 is mounted a gear 383 which is
held in place through a lock pin 382 held in aligned passageways in
the shaft 375 and the gear itself. Gear 383 turns on bearing
surface 384 and engages gear 385 which is on a second shaft 387.
This shaft is driven by the wiper 366 rotated by the wiper tape W.
Gear 385 is held on shaft 387 through lock pin 386. A projection
388 of frame 370 contains a hollow passageway 389 and journal
bearings 390 and 391. Gear 385 rides on bearing surface 393. The
upper end of shaft 387 is bored as at 393 and has a passageway
receiving a pin 394. Mounted atop this upper end of shaft 387 is
member 395 having a passageway 396 therein which receives the upper
extremity of shaft 387 and a slotted area 397 which allows pin 394
to move within member 395. A helical compression spring 398 is
mounted within passage 393 and is anchored at its upper end in a
small passage in member 395. Thus, member 395 rotates with, but is
longitudinally biased on, shaft 387 as indicated by the arrow in
FIG. 13.
A projection 399 on the top of member 395 engages in an aperture
400 in the bottom of wiper spool 366. Wiper spool 366 is mounted in
a journal bearing 401 in tray 354 and has a circular rotating base
portion 402. Four projecting tab portions 404 extend upwardly from
portion 402. Each tab 404 has a generally vertical surface with
upper and lower tab portions, such as 403, and a puncture point 405
which punctures the wiper tape W to meter the tape through the
supply reels and take-up reels. As shown in FIG. 14, frame 370 has
mounting holes such as 370' and is generally rectangular with one
edge rounded. The top of member 395 is shown in FIG. 14 and the
projection 399 is shown as equal to the diameter of the upper
member of circular member 395. The member is adapted to turn in
aperture 406 in the frame member. Thus, it is seen that when
plunger pin 193 is engaged in slot 380, it maintains the wiper
spool 366 against any further movement. Wiper spool 366
automatically engages and disengages member 395 when the wiper tray
352, FIG. 12 is moved in or out.
Referring again to FIG. 2, it is seen that the cam and cams 141 and
143 clutch mechanism 140 is held in engaging position by washer
167' and a compression spring 171' which acts on washer 168' and
surrounds shaft portion 169.
TIP AND TIP WIPER OPERATION
The tip motor (not shown) is bidirectional. The time that it turns
in either direction, thus the tip depth stroke, is controlled by a
dial setting on knob 38 on panel 36. Before the motor will reverse
its direction, however, it must rotate at least 56 1/2.degree..
This minumum angular displacement is effected by actuator bar 228
on gear 221 (FIG. 2) striking either lobe, such as 229, on member
230 which in turn actuates switch 232 (FIG. 7). The minimum angle
of displacement insures that the snap action of cylinders 261 and
261' will be completed and that one of the cam followers, such as
263, will fall in the back or front cam groove. It is apparent from
FIGS. 10a, 10b, and 10c, that the arcuate portion of each groove
(as viewed in FIG. 10a) on the cylindrical cam 216 surfaces extends
through 90.degree. rotation from one side to the center. This
represents 56 1/2.degree. rotation of the motor when the gear ratio
of the drive and driven gear is 2.0:1.25 as is preferable.
Once the cam follower has dropped in a groove, the motor may
continue to turn for any angular displacement up to 180.degree.
maximum.
An angular displacement greater that 56 1/2.degree. is timed. At
the end of a set time period, the tip stops and pauses until the
pipette operation is completed which usually takes around 3
seconds. At that time, the motor rotational direction is reversed
and the tip T is raised in a straight line until it reaches top
dead center. Past top dead center position the cam follower riding
in the groove in cam 216 is advanced sinusoidally forward, or
backward, depending on the groove the follower is in, until a
toggling action takes place, which consists of one follower riding
out of the surfacing groove and the second follower dropping into
the second groove. Naturally, all this action is transmitted to
guide bars 99' and 99" on which the top holder 100 slides. At the
same time, the motor turns crank 90 which, via cam follower 118,
moves the cross slide subassembly vertically. As can be seen from
FIGS. 1 through 4, rods 105 and 105' ride in holes in tip holder
100. In other words, the tip holder 100 is moved horizontally by
the rods 105 and 105' and vertically by the rods on the cross slide
sybassembly.
While the tip is being moved, the cam shaft 75 is being rotated in
either direction via gears 86 and 74. When the crank 90 is at top
dead center, the top lobe 151 on cam 145 (FIG. 5) holds up follower
plate 167, thus disengaging the pin 171 on the plate follower and
slot 165' in the cam plate 143. Consequently, the cam 145 rotates
with gear 74 when the drive shaft 85 and motor rotate. The cam 145
turns until stopped by the top pin 215 in follower 214. Initially,
the pin 215 rests against one of the walls of slots 163 or 165 of
the 38.degree. arc cutout. If shaft 75 rotates more than
approximately 103.degree., the radius surface 141 on cam 145 gives
way to the smaller radial surface 146. This allows the follower 214
to move vertically which raises pin 215 into either slot 163 or
164. As shaft 75 continues to rotate to the angular displacement
dictated by the time setting on the motor, the pin 215 remains in
the upper portion of either slot. The pin goes into the slot only
if more than 103.degree. shaft rotation has occurred. Presuming the
motor, hence shaft 75, reverses at some displacement greater than
103.degree., then the pin 215 will remain in its slot until the
larger cam surface 141 pushes it out. At that time, the friction
plates 142 and 144 turn the cam 145 in the direction of shaft
rotation. This results in the dropping of follower plate 167 and
dropping of pin 171 into notch 165' of cam plate 143 and locking
the cam plate with the ears horizontal. The ears 161 and 162 on cam
plate 143 push out the rollers 177 and 177' which in turn rotate
the wiper bars 131 and 131' which swing the wiper pads 137 against
the tip and 137'. This action wipes the tip T. At near top dead
center tip-slide 100, the top lobe 151 on cam 145 raises follower
plate 167 and hence pin 171. Cam plate 143 is rotated and the ears
161 and 162 move out of the horizontal position causing the wiper
pads 137 and 137' to separate. All the activity just described
results in the tip being wiped only as it is raised out of a tube
and only for a maximum distance of approximately 1.8 inches. This
is the distance from the wipe start to the end of the tip. A
shorter distance is wiped if the tip depth setting is less.
The oscillation of cam 145 causes the lever 202 to snap alternately
into its positions. Each time an this occurs, one of the plunger
pins 193 (in 207 or 184) is lifted out of slot 380 in twist disc
377, and the other descends to ride on top of the ungrooved or
unslotted portion of the disc. This action frees the disc to rotate
180.degree. before the riding pin 193 drops into the slot and locks
the disc. The disc has a constant torque on it due to take-up
tension on the wiper tape being transmitted via the wiper spool 366
and the transmission assembly 180. This snap-action of lever 202
occurs shortly after the tip T passes top dead center. Therefore,
the tape moves as the tip is entering the tube. The tape has
advanced before the tip is retracted.
Referring now to FIGS. 8, 9 and 12, a full supply reel is placed on
pin 360' and the wiper tape is routed as shown in FIG. 12. When the
tray is pushed in, the spring loaded clutch assembly shown in FIG.
9 tends to take the slack out of the tape on the supply side of
wiper spool 366 and the motor driven clutch 339 (FIG. 9) starts up
to take the slack out of the take-up side of the wiper spool. The
motor driven clutch 339 runs constantly at approximately 2
revolutions per minute. Both clutches 317 and 339 slip at a
constant torque. Therefore, the tension on each side of the wiper
spool 366 is always constant. The wiper spool 366 has four needles
405 press-fit with bushings which extend beyond the surface of
lands as 404. During a running operation, there is approximately 3
ounces of tape tension in the supply side and 8 ounces of tension
on the take-up side of the spool. Negator springs 310 and 328 are
located and sized so that the spindles 321 and 342 exert a constant
radial force on reels 360 and 361 at all tape diameters.
The tray 354 cannot be opened except when the tip is at top dead
center. In this position, the tip is up and the wiper pads 137 and
137' are spread to the maximum position. This allows the wiper
spool 366 to come out between the wiper pads and the tip assembly
housing. Once the tray 354 is in place, the transporter can be
started. As previously mentioned, the disc 377 is free to rotate
180.degree. every time the tip T passes top dead center. Since
there is a tension differential between the supply and take-up
sides of the wiper spool, the tape advances until the disc is
locked again. The tape rolling diameter of the wiper spool 366 and
the spools' relative distance from the two pad wiper stations
provides for the tape to wipe the tip at each retraction but never
twice on the same spot on the tape. Also, the wetted part of the
tape goes around the wiper spool 366 between lands 404 so that the
wiper spool is not contaminated.
ELECTRONIC CONTROLS
The electronic controls are shown in FIGS. 15-29. The overall
systems are contained in FIGS. 15, 17, 18, 19, 20, 21, 23, 25 and
26. Sample circuitry is shown in FIGS. 16, 22, 24, 27, 28 and
29.
FIG. 15 shows the A.C. motor driver circuit; FIG. 17 is the pipette
logic circuitry; FIG. 18 is the tip motor logic circuitry; FIG. 19
is the shift register and logic circuitry; FIG. 20 is the advance
and start logic circuitry; FIG. 21 is the lamp driver circuitry;
FIG. 23 shows the manual switch buffers and logic circuitry; FIG.
25 shows the automatic switch buffers and logic circuitry; and FIG.
26 shows the malfunction and alarm logic circuitry. The input and
output leads of the aforementioned circuits shown on the figures
just enumerated do not match up from figure drawing to figure
drawing but are described herein. In other words, an output lead on
FIG. 25 is described as connected to an input lead on FIG. 15, for
instance. FIG. 18, the tip motor logic circuitry, consists of two
sheets and has been designated FIGS. 18A and 18B. The latter two
drawings have aligned leads which match up when the drawing sheets
are placed together.
The remaining figures show circuitry which is representative of
circuits which are shown as boxes on the aforementioned figures.
FIG. 16 is the driver and braking circuit; FIG. 22 shows the driver
circuitry; FIG. 24 is the power-up reset circuitry; FIG. 29 is the
relay timer circuitry; FIG. 27 is the interface circuitry; and FIG.
28 is the timer circuitry.
The control system has six operator-controlled switches which are
shown in FIG. 1 and designated as 39-44. They are the Automatic
Mode Switch, the Manual Mode Switch, the Advance Mode Switch, the
Pipette Mode switch, the Reset Switch and the Start Switch. To
provide power to the electronic system, either the Run or Set
switches have to be activated on the pipette console. These
switches are shown in FIG. 1 as 26 and 27. Activation of these
switches places the pipettes in a prime sequence and provides power
to the electronic control system. Other switches which are
activated by the apparatus itself include the Advancement Switch,
Paper Reel Supply Switch, the Tray Switch, the Advance Motor Cam
Switch, the Ejection Motor Cam Switch, the Rack Limit Switch, the
Rack Sensing Switch, the Tube Sensing Front Switch, the Tube
Sensing Back Switch, the tip Up Sensor Switch, and the tip "Toggle"
Switch. Additional controls are provided by a Rear Stroke
Adjustment, a Front Stroke Adjustment, an External Select Switch,
and an External Potentiometer, and an Auxiliary Pipette Select
Switch.
Referring now to FIG. 15, the A.C. motor driver circuitry diagram,
it is seen that the circuitry consists of a group of small
individual circuits for controlling the motors. A signal enters the
advance motor circuit through terminal 511 and is an advance motor
driver signal from terminal 875 on FIG. 20. The signal proceeds
through the A.C. driver 509 and braking circuit 510 and on to the
advance motor 501 which has a capacitance 507 and is connected to
the A. C. voltage at 508. A tip motor driver signal enters at
terminal 517 and originates in the tip motor logic circuitry, FIG.
18, at terminal 730. The signal continues on through A. C. driver
515 and braking circuit 516. The signal then proceeds through a tip
reversing relay switch 514 to tip motor 502 which has a capacitance
512 and is connected to the A. C. voltage at 513.
A tip reverse relay signal enters at terminal 521 and originates in
the tip motor logic circuitry, FIG. 18, at terminal 746. The signal
continues on to reversing relay 518, which is used to reverse the
tip motor. Terminal 520 is connected to the logic voltage. The
component designated at 519 is a silicon rectifier. A pipette motor
driver signal enters at terminal 526 and orginates in the pipette
logic circuitry, FIG. 17, at termainl 638. The signal proceeds
through A. C. driver 524 and braking circuit 525 and then on to
pipette motor 503 which has a capacitance at 522 and is connected
to the A.C. voltage at 523.
An ejection motor driver signal enters at terminal 530 and
originates in the automatic switch buffer and logic circuitry, FIG.
25, at terminal 1091. The signal proceeds through A. C. driver 529
to the ejection motor 504. Motor 504 has a capacitance 527 and is
connected to the A. C. voltage 528. A tray feed bar motor driver
signal enters at terminal 534 and originates in the advance and
start logic circuitry, FIG. 20, at terminal 878. The signal
proceeds through A. C. driver 533 and on to tray feed bar motor 505
which has capacitance 531 and is connected to the A. C. voltage at
532.
A buzzer driver signal enters at terminal 538 and originates in the
malfunction and alarm logic circuitry, FIG. 26, at terminal 1172.
The signal proceeds through A. C. driver 537 to buzzer 536 which is
connected to the A. C. voltage at 535. A reel motor driver signal
enters at terminal 542 and originates in the malfunction and alarm
circuitry, FIG. 26, at terminal 1185. This signal passes through A.
C. driver 541 to reel motor 506. Motor 506 is connected to the A.
C. voltage at 540 and has a capacitance 539.
FIG. 16 shows a representative A. C. driver and braking circuit.
The input signal enters the circuit at terminal 549 and passes
through resistor 548. The signal is then modified by silicon
transistors 545 and 547 along with resistors 546, 550, 554 and 555.
The signal then passes through Triac 544 and then proceeds out at
terminal 563. Resistor 556, 558 and 559 and silicon rectifiers 557
and 561 and capacitors 560 and 562 comprise the braking circuit.
The entire circuit is shown designated as 566. Terminals 551 and
565 are ground terminals. Terminal 552 and 553 are logic voltage
connections. Terminal 564 is an A. C. voltage connection. The
various arrows shown in the lead lines are merely quick-discconnect
points so that the various components, such as the motors, can be
easily removed from the system. Obviously, the capacitance for each
of the motors, 501 thorugh 505, may vary and have a different
value.
Referring now to FIG. 17, there is shown the pipette logic
circuitry. The circuitry contains a plurality of switches which are
located in the transporter pipette and are enclosed within the
dotted line box 568. The entire circuit is designated as 567.
Within box 568 are manually operated switches Run and Set, 572 and
573, respectively. These buttons or switches are depressed to
initiate A. C. power into the transporter. When both the Set and
Run switches are depressed, the pumps within the pipette unit are
in a prime condition. Also located within the transporter are
pipette limit switches 570 and 571, switch 571 being for the left
position and switch 570 being for the right position. As shown in
FIG. 17, these contacts are normally closed. Also located within
the pipette is a pipette limit set position switch 574, normally
with closed contacts, and a pipette limit right position switch 569
which is located on the double cam within the pipette unit itself.
As shown in the circuit, switch 569 is grounded as at 569'.
Switch 569 has leads connected to a flip-flop acting as a switch
buffer and consisting of NAND gates 576, 580, capacitance 581 and
resistors 577, 578 and logic voltage 579. The output of the gates
is passed through terminal 632 to terminal 836 in FIG. 20, the
advance and start logic circuitry.
The signal made by either contact 570, 571, or 574 or switches 572
and 573 passes through an R-C filter containing resistors 582, 584'
and capacitance 584 which is grounded at 585. Resistor 582 is
grounded as at 583. The signal then passes through silicon
rectifier 586 and through a SCHMITT trigger consisting of NAND
gates 587 and 589 and resistors 588 together with capacitance 590
which is grounded at 591. The signal than passes through gate 592
and onto gates 628 and 629. The signal is added in AND gate 628 and
passes through NAND gate 634 to terminal 633, which is connected to
terminal 763 on FIG. 19, the shift register and logic circuitry.
The output of gate 629 is fed into gate 635 together with a logic
voltage signal originating in terminal 575' and is outputted
through gate 638 to terminal 526 on FIG. 15, the A.C. motor driver
circuitry. A capacitance 636 grounded at 637 is connected to the
output of gate 635.
Provision for an auxiliary pipette is made by switch 593 and the
auxiliary pipette connector 597 which is connected to the logic
voltage as 596 and 595 and has an auxiliary pipette select switch
594. See FIG. 17. If a pipette is in use, the output is passed
thorugh an R-C filter consisting of resistors 598 and 600',
capacitance 599 and grounds 598', and 599'. The signal passes
through a sCHMITT trigger comprising silicon rectifier 600, gates
603' and 603, resistance 602 and capacitance 604 which is grounded
at 605. The output of the SCHMITT trigger is fed into gate 628
together with the output of gate 592. If auxiliary devices such as
a flame spectrophotometer are used, provision is made through
terminals 606 and 607 for external control pulses which pass
through gate 608 and are fed also into gate 628.
A series of four additional signals are provided in the circuitry.
A pipette mode signal enters the circuitry at 609 and originates
from output terminal 1013 in FIG. 23, the manjal switch buffer and
logic circuitry. A start signal enters the circuitry at terminal
611 and originates from output terminal 1016 in FIG. 23, the manual
switch buffer and logic circuitry. Additionally, signals
representing the third and sixth bit from the shift register enter
the circuitry at terminals 613 and 614. respectively, and originate
from output terminals 808 and 812, respectively, in FIG. 19, the
shift register and logic circuitry. The signals from terminals 609
and 611 pass through gate 610 and are mixed with the shift register
bit signals from terminals 613 and 614 in OR gate 615. Also, the
sixth bit signal from terminal 614 is carried to a NOR gate 630 and
is combined with the outut of gate 629 through capacitance 630' and
resistance 631 to provide an output at terminal 631 which is
connected to input terminal 701 in FIG. 18, the tip motor logic
circuitry. Referring back to gate 615, the output thereof passes
through capacitors 616 and through one-shot start pulse system
comprising NOR gates 620, 622 and 624 and resistors 618 and 621
grounded at 619 and 621', respectively, and capacitance 623.
Capacitor 617 is a filter capacitor. The output from NOR gate 624
is also fed into gates 628 and 629. Additionally, the output of
gate 624 passes through a relay driver 625 and an operator
auxiliary pipette start relay 626 which is connected to the logic
voltage as at 627. Also included in the circuit is contact 639 of
626 having terminal connection points 640, 641, and 642, which is
adapted to operate like a foot switch when an auxiliary pipette is
connected into the transporter.
Referring now to FIG. 19, the shift register components are shown
designated as 797, 798, 799, 800, 801, 802, 803 and 804. The
component is an 8-bit Serial In Parallel-Out Shift Register. A
series of input terminals are shown on the left-hand side of the
drawing. A start signal enters the circuit at terminal 748 and
originates from output terminal 877 in FIG. 20, the advance and
start logic circuitry. A tube signal enters the circuitry at
terminal 760 and originates in output terminal 1041 in FIG. 25, the
automatic switch buffer and logic circuitry. An advance signal
enters the circuitry at terminal 762 and originates in output
terminal 876 in FIG. 20, the advance and start logic circuitry. A
pipette signal enters the circuitry at terminal 763 and originates
in terminal 633 in FIG. 17, the pipette logic circuitry. A tip
motor signal, for running the tip motor forward or reverse, enters
the system at terminal 768 and originates in output terminal 728 in
FIG. 18, the tip motor logic circuitry. An automatic mode signal
enters the circuitry at terminal 769 and originates in output
terminal 1008 in FIG. 23, the manual switch buffer and logic
circuitry. A manual mode signal enters the circuitry at terminal
770 and originates in output terminal 1009 in FIG. 23, also the
manual switch buffer and logic circuitry. A start signal and
automatic and manual signal enter the system at terminals 771 and
778, respectively, and originate in output terminals 1016 and 1010,
respectively, In FIG. 23, the manual switch buffer and logic
circuitry. A rack sense signal enters the system at terminal 779
and originates in output terminal 1049 in FIG. 25, the automatic
switch buffer and logic circuitry. And, finally, a CLEAR signal
enters the system at terminal 780 and originates from output
terminal 879 in FIG. 20, the advance and start logic circuitry.
The start signal entering at terminal 748 is fed through a NOR gate
749 through an A.C. coupling circuit consisting of ground 751,
resistor 752 and capacitor 750, and through NAND gate 753 and
through another A.C. coupling circuit consisting of capacitants
754, resistor 756 and ground 755. The signal proceeds through NAND
gate 757 and on to a NAND gate, which acts like an OR gate, 781. A
capacitance is attached to the connecting line between gates 757
and 781 and is designated as 759, which is grounded at 758.
The tube signal passes through NAND gate 761 and is fed to an AND
gate 767. The advance, pipette, and tip motor signals, originating
from terminals 762, 763 and 768, respectively, are fed to gate 764.
The output of NAND gate 764 is fed through NAND gate 765 to AND
gate 767, together with the signal from gate 761. The automatic
mode signal from terminal 769 is fed through an inverting NAND gate
772, through an OR gate 774, where it is coupled with the output of
an AND gate 773, and then on to gate 767. The manual mode signal
and start signal from terminals 770 and 771, respectively, are fed
into AND gate 773.
The output of gate 767 is fed through an A.C. coupling circuit
comprising capacitance 775, resistor 777 and ground 776, and fed to
OR gate 781. The output of gate 781 is fed through two NOR gates,
782 and 785, which have an A.C. circuit comprising ground 783,
resistor 784' and capacitance 786 connected thereto. The output of
gate 785 is fed through an invertor 814 and then on to the
components of the shift register.
The automatic and manual signal from terminal 778 and the rack
sense signal from terminal 779 are fed into AND gate 821 where they
are combined with the start pulse from terminal 748. The output of
gate 821 is fed through inverting NOR gate 822 and then on to OR
gate 823. The output of OR gate 823 is an advance lamp signal going
to terminal 820 which is connected to input terminal 890 in FIG.
21, the lamp driver circuit.
The output of gate 753 is additionally fed to an entry flip-flop
circuit consisting of gates 789 and 788 and capacitance 790. The
output of that circuit is fed to OR gate 791 and through NOR gate
792, NAND gate 795 and invertor 796 to the first component 797 on
the shift register. A capacitance 794 grounded at 793 is located
between gates 792 and 795. Additionally, a signal to OR gate 823 is
fed up to OR gate 791.
The connecting line between gates 791 and 823 also is connected to
components 803 and 804 of the shift register and provides a seventh
bit output from the register at output terminal 813 which is
connected to input terminal 658 in FIG. 18, the tip motor logic
circuitry. The clear signal from terminal 780 is fed through
invertor 815 into the shift register. The shift register provides
several outputs and also circulates a bit back from the shift
register from components 803, 804 into gate 791 which is connected
to gate 789 of the entry flip-flop. The bit signal from component
797 in the shift register is used to reset the entry flip-flop via
gate 787. A bit signal 1 passes through output terminal 805 and is
connected to input terminal 829 in FIG. 20, the advance and start
logic circuitry, and input terminal 886 in FIG. 21, the lamp driver
circuitry. A bit signal 2 passes through output terminal 806 which
is connected to input terminal 647 in FIG. 18, the tip motor logic
circuitry. A bit signal 3 passes through invertor 807 and through
output terminal 808 which is connected to input terminal 613 in
FIG. 17, the pipette logic circuitry. A bit signal 4 passes through
output terminal 809 which is connected to input terminal 666 in
FIG. 18, the tip motor logic circuitry and input terminal 887 in
FIG. 21, the lamp driver circuitry. A bit signal 5 passes through
output terminal 810, which is connected to input terminal 682 in
FIG. 18, the tip motor logic circuitry. A bit signal 6 passes
through invertor 811 and output terminal 812 which is connected to
input terminal 614 in FIG. 17, the pipette logic circuitry. As
stated before, a bit signal 7 passes through terminal 813 which is
connected to input terminal 658 in the tip motor logic
circuitry.
An output from components 802 and 803 of the shift register is
connected through OR gate 818 which also receives an output from
components 799 and 800 of the shift register. The output of gate
818 is a tip up lamp signal which passes through output terminal
819 connected to input terminal 889 in FIG. 21, the lamp driver
circuitry. An output from components 798 and 799 is coupled with an
output from components 801 and 802 of the shift register in OR gate
816. The output of gate 816 is a pipette lamp signal passing
through output terminal 817 which is connected to input terminal
888 in FIG. 21, the lamp driver circuitry. Referring now to FIG.
20, there is shown the advance and start logic circuitry. Several
inputs are provided on the left-hand side of the figure. A
malfunction signal enters the circuit at terminal 826 and
originates in output terminal 1183 of FIG. 26, the malfunction and
alarm logic circuitry. An advance motor cam signal enters the
circuitry at terminal 827 and originates from output terminal 1096
in FIG. 25, the automatic switch buffer and logic circuitry. The 1
bit pulse signal from output terminal 805 in the shift register
circuitry enters the advance and start logic circuitry at terminal
829. A tip up signal enters at terminal 830 and originates from
output terminal 1058 in FIG. 25, the automatic switch buffer and
logic circuitry. An advance mode signal and a start signal enter
the circuitry at terminals 831 and 834, respectively, and originate
from output terminals 1012 and 1016 in FIG. 23, the manual switch
buffer and logic circuit. An ejection signal enters at terminal 835
and originates from output terminal 1090 in FIG. 25, the automatic
switch buffer and logic circuitry. A pipette position signal enters
the circuit at terminal 836 and originates in output terminal 632
in FIG. 17, the pipette logic circuitry. A rack signal enters at
terminal 837 and originates from output terminal 1049 in FIG. 25,
the automatic switch buffer and logic circuitry. An automatic and
manual signal enters at terminal 838 and 844 and originates from
output terminal 1010 in FIG. 23, the manual switch buffer and logic
circuitry. A tube signal enters at terminal 843 and originates from
output terminal 1041 in FIG. 25, the automatic switch buffer and
logic circuitry. A reset signal enters at terminal 847 and
originates from output terminal 1015 in FIG. 23, the manual switch
buffer and logic circuitry.
The malfunction signal from terminal 826 passes to an AND gate 872.
The signal additionally is fed to AND gate 839 and OR gate 851. The
advance motor cam signal from terminal 827 is fed to a gate 869, a
NAND gate in a flip-flop, and an OR gate 871. The 1 pulse bit
signal from terminal 829 is fed through an A.C. coupling circuit
comprising capacitance 832', resistor 833' and ground 833 to an AND
gate 854. The tip up signal from terminal 830 is fed through an
inverting gate 832 and to AND gate 854 and AND gate 855. The
advance mode signal from terminal 831 and the start signal from
terminal 834 are also fed to AND gate 855. The ejection signal from
terminal 835 is also fed to AND gate 855. Additionally, the start
signal from terminal 834 is fed to an AND gate 853 and the ejection
signal from terminal 835 is fed to a NAND gate 848. The pipette
position signal from terminal 836 and the rack signal from terminal
837 are fed to AND gate 839 together with the start signal from
terminal 834, the malfunction signal from terminal 826 and the
automatic and manual signal from terminal 834. The output of gate
839 is fed through a flip-flop circuit comprising NAND gates 840
and 841 and capacitance 842. Additionally, the pipette position
signal from terminal 836 is fed through an inverting gate 828, the
output of which is coupled with the start signal pulse in AND gate
853. The output of the flip-flop concluding gates 840 and 841 is
fed also to gate 853 and to an AND gate 856, together with the
ejection signal from terminal 835. The tube signal from terminal
843 is also fed to AND gate 856. The rack signal from terminal 837
is also fed to AND gate 856 and to an inverting gate 845. The
output of inverting gate 845 is passed to a NAND gate 848 which
also receives an ejection signal from terminal 835. An A.C.
coupling circuit is provided having capacitance 849, ground 850 and
resistance 850'. The output of gate 848 is passed to an OR gate 851
which also receives a malfunction signal from terminal 826 and a
reset signal from terminal 847. Additionally, the output from
inverting gate 845 passes through an A.C. coupling circuit
comprising capacitance 845', resistor 846' and ground 846, to a
NAND gate 857. The output of OR gate 851 passes through an
inverting gate 852 and provides a clear signal at output terminal
879 which is connected to input terminal 780 in FIG. 19, the shift
register and logic circuitry. Additionally, the clear signal is fed
back to gate 841 of the flip-flop receiving the output of AND gate
839.
The output of gate 857 is fed to a flip-flop including gates 858,
860 and capacitor 859 and the output of that flip-flop controls a
three-second timer 861 which is coupled to a resistor 862 and
capacitor 863. The output of AND gate 856 is fed through two NOR
gates 865 and 866, which together act like an OR gate. Gate 865
also receives an output from the flip-flop including gates 858 and
860.
The tube signal from terminal 843 is also fed to an OR gate 867 and
is coupled with the signal output from the 3-second timer 861. The
output of AND gate 854 proceeds to a flip-flop including gates 868
and 869 and capacitor 870. The output of the flip-flop is fed to OR
gate 871. Gate 869 of the flip flop also receives the reset signal
from terminal 847 and the advance motor cam signal from terminal
829. The advance motor cam signal is also fed to OR gate 871 which
additionally receives the output of AND gate 855. The output of
gates 865 and 866 is also fed into OR gate 871. The output of gate
871 is fed through an inverting gate 873 to provide an advance
signal through at output terminal 876 which is connected to input
terminal 762 in FIG. 19, the shift register and logic circuitry. A
capacitor 874', grounded at 874, is connected to this output line.
Additionally, the output of OR gate 871 is fed through AND gate 872
which also receives the malfunction signal from terminal 826. The
output of gate 872 is fed to output terminal 875 which is connected
to input terminal 511 in FIG. 15, the A.C. motor driver circuitry.
The output of gate 871 is also fed back to gate 857, which controls
the flip-flop including gates 858 and 860. The output of AND gate
853 provides a pump sequence signal at terminal 853' which is
connected to input terminal 885 in FIG. 21, the lamp driver
circuitry. The output of gate 841 of the flip-flop is also used to
provide a start signal at output terminal 877 which is connected to
input terminal 661 in FIG. 18, the tip motor logic circuitry and
input terminal 748 in FIG. 19, the shift register and logic
circuitry. The output of the flip-flop including gates 858 and 860
is also passed out of the system as a tray feed motor signal at
output terminal 878 which is connected to input terminal 534 in
FIG. 15, the A.C. motor driver circuitry. An inverting gate 864 is
connected to the three-second timer 861 in gate 860 of the
flip-flop.
Referring now to FIG. 21, there is shown the lamp driver circuitry
designated generally as 880. The lamp driver circuitry provides an
indication to the operator of what is going to occur next and what
is happening at the present time. Additionally, it provides an
indication of problems such as jams in the system. An open tray
signal enters the system at terminal 881 and originates from output
terminal 1173 of FIG. 26, the malfunction and alarm logic
circuitry. The signal passes through lamp driver circuit 895 to the
open tray lamp 896 to give the operator warning if he is about to
operate the system and the tray is in open conditon. The lamp
driver circuits for all of these lamps are substantially identical
and are illustrated in FIG. 22 to be described.
An advance jam signal enters at terminal 882 and originates from
output terminal 1182 of FIG. 26, the malfunction and alarm logic
circuitry. The signal then proceeds on through lamp driver circuit
897 to advance jam light 898.
A tip wiper signal enters at terminal 883 and originates from
output terminal 1184 in FIG. 26 and passes through lamp driver
circuit 899 to the tip wiper lamp 900.
An ejection signal enters at terminal 884 and originates from
output terminal 1090 in FIG. 25, the automatic switch buffer and
logic circuitry, and passes through lamp driver circuit 901 to
eject lamp 902.
A pump sequence signal enters at terminal 885 and originates from
output terminal 853' in FIG. 20, the advance and start logic
circuitry, and passes through lamp driver circuit 903 to pump
sequence lamp 904.
A 1 bit signal enters at terminal 886 and originates from output
terminal 805 in FIG. 19, the shift register and logic circuitry.
The signal then proceeds through invertor 907 and lamp driver
circuit 905 to the tip down front light 906.
A 4 bit signal enters at terminal 887 and originates from output
terminal 809 in FIG. 17, the pipette logic circuitry. The signal
then proceeds through invertor 908 and lamp driver circuit 909 to
the tip down back lamp 910.
A pipette sequence lamp signal enters at terminal 888 and
originates from output terminal 817 in FIG. 19, the shift register
and logic circuitry, and passes through lamp driver circuit 911 to
pipette lamp 912.
A tip up sequence lamp signal enters at terminal 889 and originates
from terminal 819 in FIG. 19, the shift register and logic
circuitry. The signal then passes through lamp driver circuit 913
to the tip up lamp 914.
An advance sequence lamp signal enters the system at terminal 890
and originates from output terminal 820 in FIG. 19, the shift
register and logic circuitry. The signal then passes through lamp
driver circuit 915 to light advance lamp 916.
An automatic mode signal enters at terminal 891 and originates from
output terminal 1008 in FIG. 23, the manual switch buffers and
logic circuitry, and then passes through invertor 919 and lamp
driver circuit 917 to the automatic mode lamp 918.
A manual mode signal enters at terminal 892 and originates from
output terminal 1009 in FIG. 23, the manual switch buffer and logic
circuitry. The signal then proceeds through invertor 920 and lamp
driver circuit 921 to the manual lamp 922.
An advance mode signal enters at terminal 893 having originated in
the manual switch buffer and logic circuitry, FIG. 23, at output
terminal 1012. The signal then passes through invertor 923 and lamp
driver circuit 924 to the advance mode lamp 925.
A pipette mode signal originates from output terminal 1013 in FIG.
23, the manual switch buffer and logic circuitry, and enters at
terminal 894. The signal then proceeds on through invertor 926 and
lamp driver circuit 927 to pipette mode lamp 928.
FIG. 22 is representative of the lamp driver circuits used in FIG.
21. Generally, the circuit is designated by 929 and the signal
enters the system at terminal 932. Terminals 935 and 941 are
connected to ground and terminal 930 is connected to the logic
voltage. The signal is then processed by resistors 931, 933, 934,
937 and 938 and silicon transistors 936 and 939 to output to the
appropriate lamp at output terminal 940.
Referring now to FIG. 23, there is shown the manual switch buffers
and logic circuitry designated as 942. There are no inputs to this
system, only outputs. The system circuitry is centered around the
four major operator switches in the transporter circuitry, namely
the automatic mode switch 943, the manual mode switch 949, the
advance mode switch 955, the pipette mode switch 961, the reset
switch 967 and the start switch 977. Each of these switches have
ground connections such as 944, 950, 957, 962, 968 and 978. There
are switch buffering circuits associated with each major operator
control switch. Switch 943 is buffered through gates 946 and 947
and resistors 945 and 948. Switch 949 is buffered through gates 952
and 953 and resistors 951 and 954. Switch 955 is buffered through
gates 958 and 959 and resistors 956 and 960. Switch 961 is buffered
through gates 964 and 965 and resistors 963 and 966. Switch 967 is
buffered through gates 969 and 971 and resistors 970 and 972.
Switch 977 is buffered through gates 980 and 981 and resistors 979
and 982. Each resistor is connected to the logic voltage at 983. A
power up reset 973 has its output connected to invertor gate 976
and is combined in gate 974 with the output from the buffering
circuit for switch 967. The outputs of the buffering circuits for
switches 943, 949, 955 and 961 are fed through a series of gates
which are designated respectively as 984, 988 and 992 for the
automatic mode switch, 985, 989 and 993 for the manual mode switch,
986, 990 and 994 for the advance mode switch, and 987, 991 and 995
for the pipette mode switch. The output of gate 992 is fed into a
flip-flop mode circuit consisting of gates 996 and 997 and
capacitor 1004. The output of this flip-flop exits through terminal
1008 in the form of an automatic mode signal and is connected to
input terminal 891 in FIG. 21, the lamp driver circuit, and input
terminal 769 in FIG. 19, the shift register and logic circuit. The
output of gate 993 is fed through a flip-flop circuit consisting of
gates 998 and 999 and capacitor 1005 and is coupled with a portion
of the output of gate 997 in an OR gate 1011 which produces an
output signal. The output signal is an automatic or manual signal
which exits through output terminal 1010 which, in turn, is
connected to input terminal 838 in FIG. 20, the advance and start
logic circuitry, and input terminal 778 in FIG. 19, the shift
register and logic circuit. The output of gate 994 proceeds through
a flip-flop circuit consisting of gates 1000 and 1001 and capacitor
1006. The output of this flip-flop circuit is an advance signal
exiting through output terminal 1012 which is connected to input
terminal 893 in FIG. 21, the lamp driver circuit, and input
terminal 831 in FIG. 20, the advance and start logic circuitry.
The output of gate 995 passes through a flip-flop circuit
comprising gates 1002 and 1003 and capacitor 1007 and exits as a
pipette signal at termianl 1013 which is connected to input
terminals 894 in FIG. 21, the lamp driver circuitry, and input
terminal 609 in FIG. 17, the pipette logic circuitry. The output of
gate 974 proceeds through inverting gate 975 to produce a pulse
signal at terminal 1015. The reset signal from gate 975 also
connects to gates 984, 985, 986 and 987 to clear the mode
flip-flops. Terminal 1015 is connected to input terminal 1119 in
FIG. 26, the malfunction and alarm logic circuitry, input terminal
847 in FIG. 20, the advance and start logic circuitry, and input
terminal 656 in FIG. 18, the tip motor logic circuitry. A capacitor
1014', grounded at 1014, is connected to the output terminal.
The output of the buffering circuit for the start switch 977
produces a start switch signal at terminal 1016. Terminal 1016 is
connected to input terminal 771 in FIG. 19, the shift register and
logic circuitry, input terminal 834 in FIG. 20, the advance and
start logic circuitry, and input terminal 611 in FIG. 17, the
pipette logic circuitry.
Referring now to FIG. 25, there is shown the circuitry for the
automatic switch buffers and ejection logic. The circuitry is
designated generally as 1031. A pair of tube sensing contacts, 1034
and 1035, have normally open contacts and act in conjunction with a
pair of tube sensing contacts 1032 and 1033, having normally closed
contacts for sensing tubes in the front and rear rows of a tube
rack. This tube sensing circuit is grounded at 1036 and signals
produced thereby are buffered through gates 1038 and 1040,
resistors 1037 and 1039 and capacitor 1041'. The output of the
switch buffer is a tube present signal exiting through output
terminal 1041 which is connected to input terminal 760 in FIG. 19,
the shift register and logic circuitry, and input terminal 843 in
FIG. 20, the advance and start logic circuitry.
A rack sensing switch 1042 is grounded as at 1043 and produces a
signal when a rack is in position which is buffered through gates
1045 and 1047, resistors 1044 and 1046 and capacitor 1048. The
output of the buffering produces two signals. The first signal is a
rack sense signal exiting at output terminal 1049 which is
connected to input terminal 779 in FIG. 19, the shift register and
logic circuitry and input terminal 837 in FIG. 20, the advance and
start logic circuitry. The second signal is a rack sense signal
exiting through terminal 1050 which is connected to input terminal
1114 in FIG. 26, the malfunction and alarm logic circuitry.
A tip up sensing switch 1051 is grounded as at 1052 and is buffered
through a buffering circuit comprising gates 1055 and 1056,
resistors 1053 and 1054 and capacitor 1057. The output of this
buffering circuit produces a tip up signal at terminal 1058 which
is connected to input terminals 659 in FIG. 18, the tip motor logic
circuitry, and input terminal 830 in FIG. 20, the advance and start
logic circuitry.
A toggle switch 1059, used to sense the toggle position on the tip
motor cam, is grounded as at 1060 and produces a signal which is
buffered through gates 1062 and 1064, resistors 1061 and 1063 and
capacitor 1065. The output of the buffering produces a toggle
signal at terminal 1066 which is connected to input terminal 662 in
FIG. 18, the tip motor logic circuitry.
An ejection motor cam switch 1067 is grounded as at 1068 and
produces a signal which is buffered through a buffering circuit
which includes gates 1070 and 1072, resistors 1069 and 1071 and
capacitor 1073. A rack limit switch 1074 which is grounded as at
1075 produces a signal which is buffered through a buffering
circuit containing gates 1077 and 1085, resistors 1076 and 1083,
and capacitor 1086. The combined signals from the latter two
buffering circuits are fed into an OR gate 1087.
An advance motor cam switch 1081 is grounded at 1082 and produces a
signal buffered through gates 1092 and 1093, resistors 1083' and
1084 and capacitor 1094. The output from gate 1092 is an advance
motor cam signal which exits at terminal 1096 which, in turn, is
connected to input terminal 827 in FIG. 20, the advance and start
logic circuitry. A common buss is connected to all the buffering
circuits and has its terminal to the logic voltage at 1078. Another
logic voltage terminal 1095 is connected to AND gate 1089 together
with the output from OR gate 1087. The output of gate 1089 produces
an ejection motor driver signal at terminal 1091. Terminal 1091 is
connected to input terminal 530 in FIG. 15, the A.C. motor driver
circuitry.
The output from OR gate 1087 is also connected to gate 1088 whose
output produces an ejection signal at terminal 1090. Terminal 1090
is connected to input terminal 1113 in FIG. 26, the malfunction and
alarm logic circuitry, input terminal 884 in FIG. 21, the lamp
driver circuitry, and input terminal 835 in FIG. 20, the advance
and start logic circuitry.
Referring now to FIG. 26, the malfunction and alarm logic circuitry
is shown designated generally as 1112. An ejection signal enters
the circuit through terminal 1113 and originates in output terminal
1090 in FIG. 25, the automatic switch buffer and logic circuitry. A
rack sense signal enters through terminal 1114 an originates from
output terminal 1050 in FIG. 25, the automatic switch buffer and
logic circuitry. A reset signal enters through terminal 1119 and
originates in output terminal 1015 in FIG. 23, the manual switch
buffer and logic circuitry. The ejection signal from terminal 1113
is passed through a NAND gate 1115, through an A.C. coupling
circuit which includes capacitor 1115', resistor 1118 and ground
1117 to an AND gate 1116. The rack sense signal from terminal 1114
is also fed into gate 1116. The reset signal from 1119 is fed
through NAND gate 1120 and NAND gate 1121. The combined outputs of
gates 1116 and 1121 are fed into a flip-flop circuit comprising
gates 1122 and 1123 and capacitor 1124. The output of the flip-flop
circuit is fed to gate 1150 which acts like an OR gate and from
there to a flip-flop circuit including gates 1155 and 1156 and
capacitor 1157. Another portion of the output from gate 1122 is fed
through an A.C. coupling circuit including capacitor 1151, resistor
1153 and ground 1152 to a NAND gate 1154 and into a gate 1155 in
the flip-flop circuit. Additionally, the output from gate 1122 is
fed to an AND gate 1162. The output from NAND gate 1121 is also fed
into the gate 1156 of the second flip-flop circuit. The flip-flop
circuit controls a seven-second timer 1160 which includes
capacitance 1159 and resistor 1158. The output from the
seven-second timer 1160 rpoceeds through gate 1161 to a second
flip-flop circuit including gates 1165 and 1166 and capacitance
1167. Also the output from gate 1121 is bypassed around the
seven-second timer to gate 1166 of the third flip-flop circuit. The
output of the latter mentioned flip-flop circuit controls a
35-second timer 1170 which includes capacitance 1169 and resistor
1168. The output from the timer 1170 is fed through a NAND gate
1171 whose output is recycled back to gate 1166. The output of
timer 1170 is also fed back to gate 1150, an OR gate.
A portion of the output of the flip-flop gate 1155 is fed to gate
1162 together with the output from gate 1122.
The output from NAND gate 1120 is fed to a series of NOR gates
1129, 1134 and 1140. These NOR gates are coupled with NOR gates
1128, 1133 and 1139, respectively, and together with capacitors
1128', 1135 and 1141 comprise switch buffering circuits. A tray
open contact switch 1125, coupled to the logic voltage at 1126
provides an input into gate 1128. A jam contact switch 1130,
connected to the logic voltage at 1131, provides an input to gate
1133. A phototransistor 1136, grounded at 1137, provides an input
into interface unit 1138. The interface unit is shown in FIG. 27.
The output from the interface unit is passed into NOR gate
1139.
A switch 1144, which is actuated when the tray is shut and if a
reel is in place, is connected to the logic voltage at 1145 and
provides a signal to either NOR gate 1142 or NOR gate 1143. The
latter two gates together with capacitance 1148 provide a buffering
for the signal. The switch buffering resistors 1127, 1132 and 1146
are grounded at 1149. The output from the buffering circuit which
includes gates 1128 and 1129 provides a tray open signal at
terminal 1173 which is connected to input terminal 881 in FIG. 21,
the lamp driver circuitry. The output from NOR gate 1133 of the
buffering circuit provides an advance jam signal at terminal 1182
which is connected to input terminal 882 in FIG. 21, the lamp
driver circuitry. The output from gate 1139 provides a tip wiper
signal at terminal 1184 which is connected to input terminal 883 in
FIG. 21, the lamp driver circuitry. The output from gate 1142 of
the buffering circuit provides a reel motor signal at terminal 1185
which is connected to terminal 542 in FIG. 15, the A.C. motor
driver circuitry. The tray signal from gate 1128 and the jam signal
from gate 1133 are also connected via OR gate 1174, whose output
goes to inverting gate 1175. The output of inverting gate 1175
passes through capacitor 1176 and into a flip-flop circuit
comprising gates 1177 and 1180 and capacitor 1181. Capacitor 1176
together with resistor 1178 and ground 1179 comprises an A.C.
coupling circuit. The flip-flop gate 1180 additionally receives an
input from gate 1121. The output of the flip-flop provides a
malfunction signal at terminal 1183 which is connected to input
terminal 826 in FIG. 20, the advance and start logic circuitry, and
input terminal 646 in FIG. 18, the tip motor logic circuitry. This
signal is also passed into OR gate 1163 together with the output
from gate 1162. The output of gate 1163 is inverted by gate 1164
and produces a buzzer signal at terminal 1172 which is connected to
input terminal 538 in FIG. 15, the A.C. motor driver circuitry.
Referring now to FIG. 18a is shown one-half of the tip motor logic
circuitry. A malfunction signal enters at terminal 646 and
originates in output terminal 1183 in FIG. 26, the malfunction and
alarm logic circuitry. The malfunction signal continues on to AND
gate 729. Since the stroke of the tip is dependent upon the time
the motor runs, a front stroke adjustment is provided as at 645 and
provides an input into a down front timer 724. Also included in
this circuit are maximum stroke adjustment 721 and minimum stroke
adjustment 722 which are located within the device and are not
operator accessible. These are set before the unit is delivered.
Down front timer 724 also includes resistor 722' and capacitor 723.
The down front timer controls the amount of time the motor is to
run and consequently the length of stroke of the tip. The entire
unit, together with the minimum and maximum stroke adjustments
constitutes a bistable multivibrator.
A 2-bit signal from the shift register in FIG. 19 enters at
terminal 647, passes through A.C. coupling circuit including
capacitor 648, resistor 649 and ground 650 and into inverter 651. A
reset pulse signal enters the system at terminal 656 and originates
from output terminal 1015 in FIG. 23, the manual switch buffer and
logic circuitry. The 2-bit signal in terminal 647 originates from
output terminal 806 on FIG. 19, the shift register circuitry. The
reset signal passes through two inverters 655 and 677 and, together
with the output from inverter 651 into a flip-flop circuit
comprising gates 652 and 653 in capacitor 654.
A 7-bit signal enters the system at terminal 658 and originates in
output terminal 813 in FIG. 19, the shift register and logic
circuitry, and enters the gate 660. A tip up signal enters at
terminal 659 and originates from output terminal 1058 in FIG. 25,
the automatic switch buffer circuitry. The tip up signal is
combined with the 7 bit signal in gate 660. A start signal enters
at terminal 661 and originates from output terminal 877 in FIG. 20,
the advance and start logic circuitry. A toggle signal enters at
terminal 662 and originates from output terminal 1066 in FIG. 25,
the automatic switch buffer and logic circuitry. The start signal
and toggle signal are fed into a gate 665. The signals are
additionally combined in gate 664 together with the tip up signal
from terminal 659, but the toggle signal from 662 is inverted by
passing through inverter 663. A 4-bit signal enters at terminal 666
and originates from output terminal 809 in FIG. 19, the shift
register and logic circuitry. The 4-bit pulse signal, together with
the tip up signal from terminal 659, are combined in gate 667.
The toggle switch signal present at 662 is AND gated with the
output of gate 652 by gate 654'. The output of gate 654' connects
to the input of timer 724.
The output from gate 653, 660 and 664 are fed into gate 668. The
output from gate 653 and gate 664 are fed into gate 669. The output
from gate 669 is fed through an A.C. coupling circuit comprising
resistor 671, capacitor 671' and logic voltage connection 670 to a
negative OR gate 678. The output from gate 665 is fed to a gate
673. The gate 673 also receives part of its input from AND gate 672
connected to leads 672' and 673' which match up with adjoining
leads on FIG. 18b.
Gate 653 also receives a portion of its input through matching lead
655'. A matching lead 665' is coupled with the output from gate 665
before entering gate 673. A gate 677 receives output from the
components on FIG. 18b through terminals 674' and 677'. The output
from terminal 677' also enters gate 673. The output from gate 673
passes through an A.C. coupling circuit comprising capacitor 674,
resistor 676 and logic voltage connection 675 to negative OR gate
678. The output from gate 677 is passed through inverting gate 726'
and onto OR gate 726. OR gate 726 receives the output also of gate
668 through inverting gate 679. The output of gate 726 is connected
to gate 729 and to gate 727. Gate 727 inverts the signal and
provides a signal at terminal 728. Terminal 728 is connected to
input terminal 768 in FIG. 19, the shift register and logic
circuitry.
The output of gate 678 is passed to the circuitry in FIG. 18b
through matching lead 678' and to NOR gate 731. Gate 731, together
with gate 732, capacitor 734, and resistor 736, grounded at 735,
provides a one-shot pulse to gate 729. The gate 729 provides a tip
motor drive signal at terminal 730 which is connected to input
terminal 517 in FIG. 15, the A. C. motor driver circuit.
Referring now to FIG. 18b, the remainder of the tip motor logic
circuitry is shown. As FIG. 18b shows a front stroke adjustment,
FIG. 18b shows a rear stroke adjustment 681 which is used to
control the stroke of the tip going into the back tube as opposed
to the front tube in the rack. Adjustment 681 provides input to a
timer circuit 690 containing resistors 688' and capacitor 689. As
in the front stroke adjustment, a maximum stroke adjustment 687 and
a minimum stroke adjustment 688 are provided within the timing
circuit. However, these latter adjustments are set and are not
operator accessible
A 5 bit signal enters at 682 from output terminal 810 in FIG. 19,
the shift register and logic circuitry. Bit 5 signal passes through
an A. C. coupling circuit comprising capacitor 681', resistor 683'
and ground 682' to an inverter 683. The output of inverter 683
together with the output of inverter 655 in FIG. 18a, is passed
into a flip-flop circuit containing capacitor 686 and gates 684 and
685. The inverted toggle switch signal present at the output of
inverter 663 in FIG. 18a connects to gate 685' via lead 690'This
signal is AND gated with the output of gate 684 by 685'. The output
of gate 685' connects to the input of timer 690. The output of
timer circuit 690 passes through inverting gate 691 and is recycled
back into the flip-flop gate 685 to reset the flip-flop. The output
of timer circuit 690 is passed into AND gate 696. Terminal 692 is
connected to resistor 694 with ground 693 to inverting gate 695. An
external select switch is coupled to terminal 692 when other
devices are to be used in conjunction with the operation of the
transporter, such as a flame spectrophotometer, etc.
An external potentiometer 700 is used in conjunction with an
external select switch when it is connected to terminal 692.
A delay start signal enters at terminal 701 and originates in
output terminal 631 in FIG. 17, the pipette logic circuitry. The
delay start signal goes to gate 705. Gate 705 also receives an
external select signal hooked to terminal 692 and its output enters
a flip-flop circuit comprising gates 706 and 707 and capacitor
707'. This flip-flop circuit is used to control and set a
one-second timer 708. Timer 708 includes capacitor 709 and resistor
710. The output of timer 708 passes through inverting gate 711 and
into a second flip-flop circuit comprising gates 712 and 713 and
capacitor 713'. The output of inverting gate 711 is also fed back
into the flip-flop gate 707. The second flip-flop circuit controls
a variable timer 714 which includes resistor 716 and capacitor 715.
The variable timer is connected to the external potentiometer 700
and is set by said potentiometer. The output of timer 714 is fed
through inverting gate 717 and onto terminal 718 to provide an
external pulse signal which tells an external device, such as a
spectrophotometer, to start. Also, the output of gate 717 is fed
back into flip-flop gate 713 to reset the flip-flop circuit. The
second flip-flop circuit is also connected via matching terminal
677' to gates 673 and 677 in FIG. 18a.
The output of inverting gate 725 in FIG. 18a is passed to a gate
697 in FIG. 18b via matching terminal 725'. Gate 697 is one-half of
a flip-flop circuit to run the motor in a forward direction, the
other half of the flip-flop being gate 698. The output of gate 696
is fed into gate 698 together with the output of inverting gate 717
in the variable timing circuit. The output of the flip-flop
comprising gates 697 and 698 are fed to an OR gate 719 together
with the output of gate 665 in FIG. 18a via matching terminal
665'.
The output of OR gate 678 in FIG. 18a is fed, via matching terminal
678' to a NOR gate 737. The output of NOR gate 737 is fed to an
inverting NOR gate 741 and the output of said second NOR gate is
fed through an A.C. coupling circuit back into gate 737. The A.C.
coupling circuit includes capacitor 740, resistor 739 and common
ground connection 738. The output of NOR gate 741 is fed to NAND
gate 743 together with the output of an inverting gate 720 which
inverts the output of OR gate 719. The output of gate 737, together
with the output of OR gate 719 are fed to a NAND gate 742. The
outputs of NAND gates 742 and 743 are fed to NAND gate 744. The
output of gate 744 is fed to a relay driver 745 which produces a
reverse relay signal at terminal 746. Terminal 746 is connected to
input terminal 521 in FIG. 15, the A. C. motor driver circuit.
ELECTRONIC SOLID STATE CONTROL SYSTEM OPERATION
The A. C. motor driver circuitry in FIG. 15 is essentially
self-explanatory in the sense that when the system receives signals
from the other circuit cards or circuitry they energize the
appropriate motors.
In FIG. 17, the pipette logic circuitry, the switches 569, 570,
571, 572, 573, and 574 are all located within the transporter
pipette. In normal operation the RUN switch 572 is closed which
selects the switches 571 and 570. Whenever the transporter pipette
is not running after a dispensing or discharging cycle, either
switch 571 or 570 will be operated causing no signal to be present
at the filtered Schmitt trigger circuit comprised of gates 587 and
589. Whenever the SET switch 573 is operated for setting the pump
stroke for the pipette, switch 574 is selected and will cause the
pipette to operate until switch 574 is operated by cam action.
Whenever both switches 572 and 573 are selected, a condition is
relaized where a signal is always present which will cause the
pipette to operate continually, thus enabling pumps to be primed.
The circuitry also accommodates the auxiliary pipette which has its
contact switch designated as 593. The various arrows accompanying
the circuit are merely pin connections as they are throughout the
remainder of the circuits. Whenever the auxiliary pipette is not
operating, switch contact 593 is closed. This causes a signal to be
present at the input of the filtered Schmitt trigger comprised of
gates 603' and 603. The auxiliary pipette select switch 594 is used
to select contact 593, otherwise, a true signal is always presented
to the said Schmitt trigger. The one-shot circuit consisting of
gates 620, 622, and 624 produces a pulse whose duration is long
enough to run the pipette to operate the cam switches in subcircuit
568 in the pipette. The said one-shot circuit is triggered by the
start switch signal when the pipette mode is selected via gates 610
and 615 and by bit signals 3 and 6 from the shift register via
terminals 613 and 614 and gate 615, an OR gate. The said one-shot
circuit energizes relay coil 626 via relay driver 625. Relay 626
has contacts 639 which operate the auxiliary pipette via terminals
640, 641, and 642.
Gate 608 is used to enable an external device to inhibit
transporter operation via gate 628 and 634 and terminal 633. Gates
628 and 634 gives a signal that indicates that the transporter
pipette or the auxiliary pipette, if selected, are operating or
that the external device, if selected, is functioning. Gates 629
and 635 are used to activate the transporter pipette motor via
terminal 638 which connects to the A. C. drivers. Gate 630 is used
to produce a delay start signal only after the transporter pipette
has completed a discharge cycle. Gates 576 and 580 are used as a
switch buffer for the right position limit switch 569 contacts.
Referring now to FIGS. 18a and 18b, the tip motor logic circuitry,
the malfunction signal originating into the system at terminal 646
prevents the tip motor from running. Gates 652 and 653 constitute a
timer flip-flop for starting the tip down front timer 724. Gate 725
constitutes a time output gate for timer 724. Each timer in all the
circuits in this apparatus has such a timer flip-flop and output
gate associated therewith. The purpose of gate 725 is to produce a
reset signal for its respective timer flip-flop, gates 652 and 653.
Whenever a timer flip-flop is set, its respective timer will start
timing. At the end of the predetermined time interval a pulse will
appear at its output which in turn resets the timer. This is true
for all timers in the apparatus. The timer flip-flop for the tip
down front timer is set by the 2-bit signal from the shift register
via terminal 647. The timing of the tip motor starts after the
toggle switch is operated by the tip motor via gate 654'. The tip
down front timer controls the time the motor runs the tip down into
the front tube. The timing interval is determined by the setting of
control 645.
The operation of the tip down back timer is identical to that of
the tip down front timer except that it is triggered by 5 bit
signal from the shift register via terminal 682 and that the timing
of the tip motor starts after the toggle switch is again operated
by the tip motor via gate 685'.
In the normal operation of the tip motor, that is, when the
external control is not selected, the motor reversing flip-flop
comprised of gates 697 and 698 is always set after a timed forward
rotation of the motor and always reset after a timed reverse
rotation of the motor. This action predetermines the next direction
of rotation. The output of the self-positioning circuitry
consisting of gates 664 and 665 also determines the direction of
rotation of the tip motor by combining with the output of the motor
reversing flip-flop via gate 719. This signal is exclusively OR'ed
with the signal from the one-shot circuit comprised of gates 737
and 741 which energizes the tip motor reversing relay via relay
driver 745 and terminal 746. The exclusive OR function is
accomplished by gates 720, 742, 743, and 744. The said one-shot
circuit is normally triggered only after the tip motor runs due to
the self-positioning circuitry or due to a timer action. The
triggering logic is accomplished with gates 669, 673, and 678. The
net result of the exclusive OR logic is to prevent the operation of
the reversing relay during dynamic motor braking. Further, the
one-shot circuit composed of gates 731 and 732 is also triggered at
the same instant as the one-shot circuit previously described. The
action of this one-shot inhibits the starting of the tip motor
until the aforesaid relay contacts are closed.
The gates 660 and 667 are used to raise the tip up from the back
and front tubes respectively upon command from the shift register
via terminals 658 and 666 respectively. Logic comprising gates 668,
677, 726, 726', and 727 gives a signal when the tip motor is
operating. The inverse of this signal is AND gated with the
malfunction signal at 646 and with the output of one-shot composed
of gates 731 and 732 by gate 729 to give a tip motor drive signal
at 730.
When the external control select switch is operated, the normal
operation of the tip is interrupted as described below. After the
tip moves down into the back tube, the normal resetting of the
motor reversing flip-flop is inhibited by gate 696. Also, the
triggering logic for the previously described one-shot circuits is
inhibited by gate 672. After a discharge cycle of the pipette, a
delay start signal at 701 starts timer 708 via gate 705. Timer 708
has a delay of approximately one second to allow any reagents that
the pipette has dispensed to mix with the sample. After the delay
of approximately one second, timer 708 starts timer 714. This timer
is adjusted through the external potentiometer 700 and makes the
tip motor run the tip further down the same tube. In other words,
the tip initially discharges above the liquid level in the tube,
then waits for a predetermined amount of time as set into the timer
714, usually one-half second, and then goes down into the liquid to
aspirate the combined liquid and sample. The external signal going
out of the circuit at terminal 718 tells the external device to
begin its function.
The reset signal at 656 is buffered by inverters 677 and 655 and is
used to reset all timer flip-flops and the motor reversing
flip-flop in the tip motor logic system.
Referring now to FIG. 19, the shift register and logic circuitry,
the shift register is used to sequentially control the operations
of the apparatus by the stepwise circulation of a single bit
through it. The appearance of the bit at each bit position
initiates the corresponding action required at that step.
The entry of the single bit into the shift register is accomplished
by an entry flip-flop comprised of gates 789 and 788, and pulse
delaying gates of 749, 753, and 757. When a start signal is
presented at terminal 748, a negative start pulse at gate 753 sets
the entry flip-flop. At the output of gate 757, a negative delayed
start pulse triggers a one-shot circuit comprised of gates 782 and
785 which produces a shift signal for the shift register. Since an
input signal is presented to the shift register from the entry
flip-flop through gates 791, 792, and 795, a bit will be entered
into the first bit position. The bit appearing at position 1 resets
the entry flip-flop.
Subsequent shift pulses are produced by the completion of an
advance operation, a tip operation, a pipette operation, or an
external operation or by manual means.
The logic consisting of gates 761, 764, 765, 767, 772, and 774 is
used to generate shift pulses when in the automatic mode upon
completion of any advance, tip, pipette, or external control
operation. The logic is also adapted to give shift pulses when the
start switch is depressed while in the manual mode. This is
accomplished specifically by gate 773.
Gates 821 and 822 are used to give an advance lamp signal before
starting occurs. Gate 823 OR's this signal with that from the shift
register so that the advance lamp will operate after starting at
the appropriate time. The lamp driver gates 816 and 818 are
connected to act like OR gates.
Referring now to FIG. 20, the advance and start logic circuitry,
the malfunction signal originating at terminal 826 is used to
inhibit the advance motor. Gate 853 is used to give a pump sequence
signal when starting is attempted when the pipette is to perform a
discharge cycle. This signal is inhibited after starting occurs.
Gate 839 is used to determine the condition which must be met
before the start flip-flop composed of gates 840 and 841 can be
set. The conditions which must be met are a false pipette right
position signal at terminal 836, a true rack signal at terminal
837, a false malfunction signal at termnal at 826, a true automatic
or manual signal at terminal 838, and a true start switch signal at
terminal 834. Once the start flip-flop is set, a 1 bit signal will
appear at terminal 829 setting the advance start flip-flop
comprised of gates 868 and 869. Gate 854 insures that the tip is up
before rack advancing occurs. After the advance start flip-flop is
set, gate 871 gives an advance motor drive signal at terminal 875
through gate 872. The signal furnished at terminal 876 through gate
873 is for the shift register logic. Once the advancement motor
starts rotating an advance motor cam switch signal will disappear
at terminal 827. This action will reset the advance start flip-flop
via gate 869 and furnish another signal to gate 871. This will
cause the advance motor to continue running until the signal at 827
is again true. The advance motor cam switch signal at 827 will only
be true after the advance motor causes one complete cycle of rack
advancement.
The advance start flip-flop is also set by another means by gate
867 as described below. Whenever a rack advances to its last tube
position, the rack signal at terminal 837 disappears causing timer
861 to start by means of gates 845 and 857. The rack signal must
change during an advance cycle in order for timer 861 to be
triggered. This is accomplished with gate 857. If no tubes are
present in the last position of the rack, the automatic advance
signal produced by gate 856 and described below is inhibited by the
timer by neans of gate 865 and 866. Timer 861 has a time delay
which allows time for the next rack to be processed, if any, to
move into the processing position. When the delay has expired the
pulse from the timer goes to gate 867 and, if no tubes are present,
then to the advance start flip-flop which causes an advance
cycle.
Two other means are possible for causing a rack advance. Whenever a
rack is advanced and no tubes are present at the new tube position
and the start flip-flop is set and no rack eject is occurring, gate
856 gives a false signal to gate 871 via gates 865 and 866. When
the rack is moved to the last tube position, this signal is
inhibited, as mentioned above, by timer 861. The rack may also be
advanced manually via gate 855. The output of gate 855 goes false,
thus causing an advance signal, when the advance mode signal at
terminal 831 is true and when the tip up signal from terminal 830
via gate 832 is true and when the not eject signal at terminal 835
is true and when the start switch signal at terminal 834 is
true.
A clear signal is generated by the logic of gate 851 which acts
like an OR gate to combine the reset signal at terminal 847, the
malfunction signal at terminal 826, and the output of gate 848. The
output of gate 848 gives a signal at the completion of the rack
processing, that is, after a rack eject occurs with no more racks
present to be processed. Therefore, a clear signal occurs at
terminal 879 when a reset, a malfunction, or a complete signal is
received by gate 851. This signal is used to reset the start
flip-flop and the shift register via terminal 879.
The operation of the lamp driver circuit shown in FIG. 21 is
relatively self-explanatory in the sense that any signal coming in
through the lamp driver circuits operates the lamp to inform the
operator of the present or impending condition. The manual switch
buffer and logic circuitry shown in FIG. 23 is also fairly
self-explanatory in that the system allows only one mode flip-flop
to be set at a time. The system essentially comprises the six main
control switches, the switch buffering flip-flops, the gates which
permit only one control flip-flop to be set at a time, and the
exclusionary control flip-flops inlcuding gates 996, 997, 998, 999,
1000, 1001, 1002 and 1003. The power up reset 973 resets the entire
logic of the transporter when the run or set switch in the pipette
module is operated. Rferring now to FIG. 25, the automatic switch
buffer and ejection logic system, the system contains a tube
sensing switch 1034 and 1032 for the front row and a tube sensing
switch 1035 and 1033 for the rear row on the rack. The signal goes
through a switch buffer comprising gates 1038 and 1040 and provides
a "tube-present" signal. The rack sensing switch 1042, the tip up
sensing switch 1051, the toggle tip switch 1059, the ejection motor
cam switch 1067, the rack limit switch 1074 and the advance motor
cam 1081 are all contacted by moving parts within the apparatus
itself and the signals produced by the switch go through switch
buffers and provide signals to the other circuits. In the case of
the ejection motor cam switch 1067 and the rack limit switch 1074,
the signals, after buffering, are combined in OR gate 1087 and
provide an ejection signal at terminal 1090 and an ejection motor
driver signal at 1089. The particular ejection mechanism is not
shown as previously stated but is simply a solenoid and motor
operated mechanism which operates two thrust push rod rack ejectors
55 and 54, as shown in FIG. 1, outwardly to eject the rack along
surface 5.
Referring now to FIG. 26, the malfunction and alarm logic
circuitry, gates 1177 and 1180 comprise a buzzer flip-flop which is
set by means of gates 1174 and 1175. The output of the buzzer
flip-flop is fed to gate 1163 which is wired as an OR gate to give
a buzzer drive signal at terminal 1172. Gates 1128 and 1129
comprise a latching switch buffer for the tray open switch 1125.
Similarly, gates 1133 and 1134 comprise a latching switch buffer
for the advance jam switch 1130. When a tray open signal or an
advance jam signal occurs, they appear at terminals 1173 and 1182
respectively to connect with the lamp driver circutriy for
malfunction indication purposes. These signals are also OR gated
together by gates 1174 and 1175 to set the buzzer flip-flop and
give a malfunction signal at terminal 1183. This is the malfunction
signal which acts to prohibit other portions of the circuitry in
the apparatus from operating. The latching switch buffers are reset
by reset signal via gate 1120 after the malfunction condition has
been cleared. The reset signal also resets the buzzer flip-flop at
gate 1180 to silence the buzzer.
The buzzer also sounds by the means described below to indicate the
completion of rack processing. After a rack has been ejected from
the processing area and no more racks are to be processed, gate
1116 sets the completion flip-flop containing gates 1122 and 1123.
When the completion flip-flop is set, gates 1150 and 1162 are
enabled and a pulse from gate 1154 sets the timer flip-flop for
timer 1160. Timer 1160 is a 7-second timer which sounds the buzzer
by means of gates 1162, 1163, and 1164. Upon the completion of the
7-second delay, timer 1160 starts timer 1170 and the buzzer is
silent. Timer 1170 is a 35-second timer which allows the buzzer to
operate intermittently. When the 35-second delay has elapsed, timer
1170 restarts timer 1160 via gate 1150 which, in turn, resounds the
buzzer for 7 seconds. This process is repeated until the operator
resets the completion flip-flop with a reset signal as at terminal
1119.
The phototransistor 1136 operates to produce a signal which is
buffered by gates 1139, 1140 to produce a tape signal at terminal
1184. This signal is indicative of a low supply of tape and
illuminates a lamp on the panel of the transporter.
The reel switch 1144 is actuated if one shuts the tray and if a
take-up reel is in place. If a reel is not in place, the take up
spindle will not run. The reel signal exits from the alarm logic
circuitry at terminal 1185.
FUNCTIONS AND APPLICATIONS
The general operating function of the transporter as stated before
is determined by the positions of the "auxiliary pipette" and
"external control" select switches on the function control panel on
the electronic module. There are the four switch combinations, in
other words, both "off," either one "on" and both "on."
With the "auxiliary pipette" and "external control" switches off,
the following combinations of operation are available: (1) a sample
on the front tube row diluted with a single reagent into the back
tube row; (2) a reagent dispensed in each tube row; or (3) one or
two reagents dispensed in the back tube row only.
With the "auxiliary pipette" switch on and the "external control"
switch off, the system is designed to operate in the arrangement
shown in FIG. 30. The tube rack 1300 contains a front tube 1301 and
a rear tube 1302 into which the tip 1303 (also shown in phantom
lines in the front tube) is designed to enter. A double pipete unit
comprising tubes 1304 and 1305 and a holder mechanism 1306 is
arranged in fixed position on the transporter. These two tubes
constitute the auxiliary dispensing pipette. Basically, the
application envisioned here is the preparation of a sample in the
tube 1301 diluted with a reagent 1 from the transporter pipette tip
1303 and with reagents 2 and/or 3 from the auxiliary pipette in
tubes 1304 and/or 1305. The transporter pipette will be manifolded
in a conventional way through a single tip moved by the tip
mechanism. The auxiliary pipette utilizes a fixed tip 1304 or tips
1304 and 1305 mounted over tube 1302 in the rear row. The auxiliary
pipette may use one or two pumps as required by the application. As
shown, the fixed auxiliary tips 1304 and 1305 do not touch the tube
well.
Referring now to FIG. 31, the device is shown adapted for the
"external control" switch on and the "auxiliary pipette" switch
off. This application requires the supply of a diluted sample to a
readout instrument. It is assumed that either the "external
control" unit or the readout unit will have means of aspirating or
pumping the sample into the readout instrument. The transporter is
provided with a twin tip shown as 1313 and 1314 in FIG. 31. Line
1316 connects with tip 1314 and is flexible and attached in turn to
a valve 1317 which connects said line with the line 1318 going to a
readout instrument. FIG. 32 shows a cross-sectional view of the
twin tip. A is evidenced from FIG. 32, the two tubes may be molded
as one having two passages. Basically, the rack 1310 contains a
front tube 1311 and a rear tube 1312. The sequence of events is as
follows: Valve 1317 is closed to tip 1314. The double tip enters
the front tube 1311 is shown in phantom lines, picks up the sample
therein and fills a diluting pump with a reagent. The tips move to
position A in the rear tube 1312, and are wiped in transit. The
sample and reagent are dispensed. Position A is preset by the
potentiometer 37 on panel 36 of the logic control module. A fixed
time delay of approximately 1 second is used to permit mixing and
liquid motion damping. The tips 1313 and 1314 then move down tube
1312 into position B, as shown in the drawings, which position is
established by an external control potentiometer as shown in the
circuitry drawings. Valve 1317 then opens to tip 1314 and the
diluted sample is aspirated. The aspiration time is set on a timer
in the external control unit. The readout is triggered at a preset
time in the aspiration cycle. Then valve 1317 closes. Valve 1317
can be three way to allow air flow through the readout instrument
if desired. At this point, controls return to the transporter logic
circuitry. The tips 1313 and 1314 are then removed from the rear
tube and wiped and the transporter operation proceeds in the usual
manner.
With the "auxiliary pipette" switch on and the "external control"
switch on, the application is the same as just described except
that the auxiliary pipette function allows the introduction of one
or two additional reagents through the fixed tips such as 1304 and
1305 in FIG. 30 during the dispensing step of tips 1314 and 1313.
In other words, the fixed pipette having the double tips shown in
FIG. 30 can be used with the double-core tip shown in FIG. 31.
With the "auxiliary pipette" switch off and the "external control"
switch on, the transporter is adapted for other readout instruments
providing minimum sample carryover and rapid sample feed to the
instrument. In this configuration, the rear tubes in the rack such
as 1320 in FIG. 33 are omitted and a fixed cup such as 1332 is
installed in the transporter on a mounting such as 1323. The
transporter has a single movable tip 1323 for sample and diluent.
The external control package is provided with a diaphragm pump 1335
to pump a solution through valve 1334 and fixed tip 1333 for
rinsing cup 1332 and is also provided with a four-way valve 1329.
Support 1323 has an aperture 1324 in the base thereof through which
a connecting stem 1325 runs to connector 1326. A pipe 1327 runs
from connector 1326 through valve 1328 and then on to valve 1329.
Conduit 1330 runs to the readout instrument such as a flame
spectrophotometer; conduit 1332 is the drain conduit, and conduit
1331 is the vent conduit.
In operation the sequence of events is as follows. Tip 1323 picks
up a sample from the front tube 1321 and the dispensing pump (not
shown) is filled. Valve 1328 is in the closed position and valve
1329 is in the position which connects the analysis instrument or
conduit 1330 to vent and the cup line 1327 to the drain line 1332.
Then tip 1323 moves to fixed cup 1332, is wiped in transit, and
discharges the sample and diluant. A short time delay is provided
for mixing and motion damping and is approximately one second. The
external control logic then takes over and initiates the following
functions. Valve 1328 opens for a preset time A allowing the
diluted sample to flush line 1327 between cup 1332 and valve 1329.
Then valve 1329 is shifted to connect the cup 1332 and the analysis
instrument connected to conduit 1330. Diluted sample is aspirated
into the analysis instrument for a preset time B and the instrument
readout is triggered. Valve 1329 shifts then to connect the conduit
1330 to vent conduit 1331, allowing the aspirator to clear the
connecting line up to valve 1329. The diaphragm pump 1335 then
pulses to rinse the fixed cup 1332 and the line 1327 between the
cup and the drain conduit 1332 clears by gravity flow. At this
point, valve 1328 is again closed and control is returned to the
transporter and normal operation proceeds. Tip 1323 is removed from
the fixed cup and is wiped. The rack then advances the next sample
tube and the operation is repeated.
While the one embodiment of the tip and wiper mechanism and solid
state circuitry has been shown and described, it will become
apparent to those skilled in the art that many changes and
modifications may be made to the apparatus and circuitry without
departing from the scope of the appended claims.
* * * * *