U.S. patent number 3,650,306 [Application Number 05/073,505] was granted by the patent office on 1972-03-21 for laboratory dispensing apparatus.
This patent grant is currently assigned to Cooke Engineering Company. Invention is credited to Jesse F. Lancaster.
United States Patent |
3,650,306 |
Lancaster |
March 21, 1972 |
**Please see images for:
( Certificate of Correction ) ** |
LABORATORY DISPENSING APPARATUS
Abstract
Laboratory dispensing apparatus including a base, a liquid
reservoir and well assembly mounted on the base, and a
microtitration plate carrier device mounted on the base for
reciprocating movement between a forward retracted position and a
rearward operative position overlying a portion of the well. A
vertically movable pipette dispensing assembly is positioned above
the base in operative alignment with the portion of the liquid well
so that with the carrier in the forward retracted position the
plural pipettes may withdraw microquantities of liquid from the
well and then dispense the same into the microtitration plate wells
when the carrier is moved to the rearward position. The plural
pipettes are supported and contained in a head unit which is
readily removable from the dispensing assembly to permit quick
changing of heat units for cleaning purposes. A liquid agitator
assembly is associated with the liquid reservoir and well assembly
to circulate and stir the liquid and thereby insure uniformity and
homogeneity of the liquid being dispensed by the plural number of
pipettes. An electrical and pneumatic control system operates the
stirring assembly and dispensing assembly, with the various control
elements and components being mounted within the base structure of
the dispensing apparatus to provide a compact self-contained
portable system.
Inventors: |
Lancaster; Jesse F. (Great
Falls, VA) |
Assignee: |
Cooke Engineering Company
(Alexandria, VA)
|
Family
ID: |
22114081 |
Appl.
No.: |
05/073,505 |
Filed: |
September 18, 1970 |
Current U.S.
Class: |
141/238; 222/263;
366/273; 141/242; 366/190; 422/922; 422/923; 422/561 |
Current CPC
Class: |
B01L
3/021 (20130101); G01N 35/1065 (20130101); B01L
3/0217 (20130101) |
Current International
Class: |
B01L
3/02 (20060101); G01N 1/00 (20060101); B65b
003/30 (); B01j 004/02 () |
Field of
Search: |
;141/8,18,21,25,20.5,31,59,67,115,129,130,181,183,234,237,238,242,392
;73/429.4R,429.6 ;23/253,259
;222/299,196,263,275,334,335,205,183,130 ;259/DIG.46 |
References Cited
[Referenced By]
U.S. Patent Documents
Primary Examiner: Bell, Jr.; Houston S.
Claims
What is claimed and desired to be secured by Letters Patent is:
1. Dispensing apparatus comprising a base section, liquid reservoir
means on said base section for containing a liquid to be dispensed,
agitator means associated with said reservoir means pick up the
liquid therein, carrier means mounted on said base section for
horizontal movement between a first non-dispensing position at one
side of said liquid reservoir means and a second dispensing
position in which said carrier means overlies a portion of said
liquid reservoir means, liquid receptacle means supported on said
carrier means, pick up and dispensing means mounted on said base
section in operative alignment above said portion of said reservoir
means, means for moving said pick up and dispensing means
vertically relative to said base section, whereby, when said
carrier means is in said first position, said pick up and
dispensing means may be lowered to a first position to withdraw
liquid from said portion of said reservoir means, then raised to
permit said carrier means to be placed in said second position, and
then lowered to a second position to dispense the withdrawn liquid
into said receptacle means.
2. Dispensing apparatus as in claim 1, said liquid reservoir means
comprising a trough positioned underneath said pick up and
dispensing means, a liquid supply well remote from said trough and
communicating therewith through connecting channel means, and said
agitator means causing agitation of the liquid in said trough.
3. Dispensing apparatus as in claim 2, wherein said channel means
comprises a pair of channels connecting said supply well with said
trough, and said agitator means causes recirculation of the liquid
between said supply well and said trough via said channels.
4. Dispensing apparatus as in claim 3, said agitator means
comprising a permanent magnet rotatably mounted in said supply well
for stirring said liquid, a drive assembly for said magnet
including motor means mounted on said base section, a drive magnet
rotated by said motor means, said drive magnet being operatively
associated with said stirring magnet to rotate the same upon
actuation of said motor means.
5. Dispensing apparatus as in claim 4, wherein said pair of
channels extends substantially tangentially from the outer
periphery of said supply well to the outer portions of said trough,
and another channel intersects and extends from the inlet one of
said pair of channels toward the central section of said trough to
ensure that all of the liquid in said trough is agitated.
6. Dispensing apparatus as in claim 1, wherein said agitator means
comprises a permanent magnet rotatably mounted in said liquid
reservoir means for stirring said liquid, a drive assembly for said
stirring magnet including motor means mounted on said base section,
a drive magnet rotated by said motor means, said drive magnet being
operatively associated with said stirring magnet to rotate the same
upon actuation of said motor means.
7. Dispensing apparatus as in claim 1, wherein said base section
includes a lower base plate and an upper mounting bed-plate
supported above said base plate, said liquid reservoir means, said
carrier means, and said pick up and dispensing means being mounted
on said upper mounting bed-plate, said mounting bed-plate having an
elongated slot at least part of which extends underneath said
reservoir means, said agitator means comprising a permanent magnet
rotatably mounted in said reservoir means for stirring the liquid
therein, motor means mounted on said lower base plate, a drive
magnet rotated by said motor means and positioned within said slot
of said reservoir means, said drive magnet being operatively
associated with said stirrer magnet to rotate the same upon
actuation of said motor means.
8. Dispensing apparatus as in claim 7, comprising an electrical
control system for said agitator motor means, a pneumatic control
system for operating said pick up and dispensing means, and at
least some of the components of said electrical and pneumatic
control systems being mounted on said base plate, thereby providing
a compact, self-contained dispensing apparatus.
9. Dispensing apparatus as in claim 1, said pick up and dispensing
means comprising manifold means, a pipette head unit including a
plurality of dispensing pipettes normally retained in assembled
relationship in said head unit, means for removably connecting said
pipette head unit to said manifold means, flexible diaphragm means
operatively positioned between said manifold means and said pipette
unit, said manifold means having air passage means leading to one
side of said diaphragm means, and a pneumatic supply system
connected to said air passage means for creating a differential
pressure on opposite sides of said diaphragm means to
simultaneously pick up predetermined quantities of liquid from said
reservoir means into said pipettes and thereafter simultaneously
dispense said quantities from said pipettes.
10. Dispensing apparatus as in claim 9, said manifold means
comprising a manifold having an air passageway, an actuating guide
plate connected to said manifold and having a plurality of
apertures communicating at one end with said manifold air
passageway and terminating at their other ends at said one side of
said diaphragm means, piston means mounted in each of said
apertures and normally biased into engagement with said one side of
said diaphragm means, said pipette head unit being removably
connected to said manifold and piston guide plate, with each
pipette being operatively aligned on the other side of said
diaphragm means with a corresponding piston.
11. Dispensing apparatus as in claim 10, said pipette head unit
comprising a dropper plate having a plurality of openings which
operatively align with said piston guide plate apertures, a
retainer plate connected to said dropper plate and having openings
aligning with said dropper plate openings, each of said pipettes
having an enlarged diameter section at one end slidably received
within one of said dropper plate openings and a smaller diameter
section extending outwardly through the aligned retainer plate
opening, and spring means biasing said enlarged diameter section
into engagement with the other side of said diaphragm means in
operative alignment with one of said pistons.
12. Dispensing apparatus as in claim 10, said means for removably
connecting said pipette head unit to said manifold means comprising
clamp means for supporting said head unit, shaft means slidably
extending through said manifold and piston guide plate and fixed at
one end to said clamp means, locking means connected to the other
end of said shaft means and movable between locked and unlocked
positions, whereby when said locking means is in said locked
position said head unit is operatively clamped to said manifold and
said piston guide plate, and when said locking means is in said
unlocked position said head unit is unclamped and may be readily
removed from said pick up and dispensing means.
13. Dispensing apparatus as in claim 12, wherein said shaft means
is adjustable screw means adjustably connected to said clamp means
so that the effective actuating pressure acting on said diaphragm
means may be varied by adjustment of said screw means.
14. Dispensing apparatus as in claim 9, wherein said pneumatic
supply system includes a vacuum source.
15. Dispensing apparatus comprising a base section including a
lower base plate and an upper mounting bed-plate supported above
said base plate; liquid reservoir means on said mounting bed-plate
for containing a liquid to be dispensed, said reservoir means
including a trough and a liquid supply well remote from said trough
and connected therewith through connecting channel means; liquid
agitator means comprising a permanent stirring magnet rotatably
mounted in said reservoir means, motor means mounted on said lower
base plate, a drive magnet rotated by said motor means and
operatively associated with said stirring magnet to rotate the same
upon actuation of said motor means; carrier means mounted on said
mounting bed-plate for movement between a first non-dispensing
position at one side of said trough and a second dispensing
position in which said carrier means overlies said trough; liquid
receptacle means supported on said carrier means; pick up and
dispensing means mounted for vertical movement on said bed-plate in
operative alignment above said trough, said pick up and dispensing
means comprising manifold means, a pipette head unit including a
plurality of dispensing pipettes normally retained in assembled
relationship in said head unit, means for removably connecting said
pipette head unit to said manifold means, flexible diaphragm means
operatively positioned between said manifold means and pipette head
unit, said manifold means having air passage means leading to one
side of said diaphragm means; a pneumatic control system connected
to said air passage means for creating a differential pressure on
opposite sides of said diaphragm means to permit simultaneous pick
up of predetermined quantities of liquid from said trough when said
carrier means is in said first non-dispensing position and then
dispensing of said predetermined quantities of liquid from said
pipettes into said liquid receptacle means when said carrier means
is in said second dispensing position; an electrical control system
for said agitator motor means; and at least some of the components
of said pneumatic control system and said electrical control system
being mounted on said lower base plate to provide a compact,
self-contained dispensing apparatus.
16. Dispensing apparatus as in claim 15, said pneumatic control
system comprising an air supply source, a solenoid-operated air
valve mounted on said lower base plate and connected between said
supply source and said air passage means in said manifold means,
manually operated electrical switch means connected to said air
solenoid valve means for operating said valve means, said switch
means being accessible to an operator, and a pressure indicator
means mounted on said base plate and extending through said
mounting bed-plate so as to be readily visible by an operator.
17. Dispensing apparatus as in claim 16, said electrical control
system comprising an electrical supply source, a transformer
mounted on said base plate, second switch means connecting the
primary side of said transformer to said electrical supply source,
said second switch means being mounted above said bed-plate for
ready access by an operator, first circuit means connecting the
secondary side of said transformer to said agitator motor means and
including a variable resistance mounted on said lower base plate
for varying the speed of said motor means, and second circuit means
including an indicator lamp mounted above said bed plate and
readily visible to an operator to indicate operation of the
apparatus when said second switch means has been placed in an on
position.
18. Dispensing apparatus as in claim 16, wherein said air supply
source is a vacuum source and said indicator means is a vacuum
gauge.
Description
BACKGROUND of THE INVENTION
This invention is related generally to laboratory analyzing and
titrating apparatus and more particularly to an automatic sampling
and dispensing apparatus capable of precisely and simultaneously
withdrawing into a plurality of needles or pipettes predetermined
reproducible microquantities of liquid from a liquid source and
delivering the same to a plurality of corresponding wells of a
microtitration plate.
In the laboratory, quite often it is necessary to perform
analytical and/or experimental tests or procedures on a large
number of laboratory specimens, the procedures frequently involving
the measuring and dispensing of microquantities of liquid in the
nature of 25 microliters. It is, of course, desirable that such
procedures be performed within a minimum amount of time and with
extreme accuracy and precision to eliminate error.
In the past, laboratory tests of this nature have usually been
performed manually and individually and have required a great deal
of time and produced questionable results. The operator simply
could not, time after time, reproduce with accuracy the
microquantitative liquid measurements necessary for these tests,
nor could he perform an adequate number of tests within a
reasonable period of time.
Automatic laboratory devices have been developed in attempting to
overcome these problems and one such device which was quite
successful is described in my copending U.S. Pat. application Ser.
No. 740,289 filed on June 26, 1968, now U.S. Pat. No. 3,568,735.
While the apparatus disclosed in application Ser. No. 740,289, now
U.S. Pat. No. 3,568,735 represented a significant improvement over
prior conventional devices, it was not entirely satisfactory from
several standpoints. For example, when the apparatus was used with
liquids consisting of two different constituents, such as a saline
solution, no provision was made in the apparatus to maintain
uniform characteristics of the liquid so that all the
microquantities of the liquid dispensed are the same. Also, each of
the dispensing pipettes was individually mounted in the pipette
dispensing assembly, thus requiring a great deal of time and labor
in removing each pipette for cleaning or replacement purposes. In
addition, the components of the electrical and pneumatic control
systems were mounted separate from the dispensing apparatus itself,
which as a result was not as compact as desired.
Accordingly, while this invention includes several of the unique
features of the apparatus disclosed in Application Ser. No.
740,289, now U.S. Pat. No. 3,568,735 it is intended to be an
improvement thereover and includes various novel features which
contribute significantly to a more reliable, more practical and
more compact dispensing apparatus.
SUMMARY OF THE INVENTION
A primary object of the invention resides in the provision of a
novel, more compact, self-contained laboratory dispensing apparatus
capable of reliable use with any type liquid and functioning to
simultaneously withdraw from a liquid reservoir into each of a
plurality of needles or pipettes a predetermined reproducible
microquantity of liquid, for example, a drop of 25 microliters, and
simultaneously dispense the same into corresponding specimen
containers or wells of a microtitration plate.
Another object of the invention resides in the provision of a more
compact, self-contained laboratory dispensing apparatus in which
the liquid supply source is readily mounted as part of the
apparatus and liquid agitating means stirs and circulates the
liquid to ensure that it is uniform and homogeneous throughout,
thereby ensuring that the plural number of pipettes withdraw
precise microquantities of liquid identical in characteristics and
dispense the same into the associated microtitration plate
wells.
Still another object resides in the provision of a novel, more
compact, and self-contained laboratory dispensing apparatus in
which the electrical and pneumatic control components for
automatically controlling a dispensing operation are mounted within
the base section of the apparatus as an integral part of the system
thereby producing a readily portable apparatus which is easier to
handle and conserves laboratory space.
A further object resides in the provision of a novel pickup and
dispensing assembly including a pipette head unit having a
plurality of needles or pipettes mounted therein, with the pipette
head unit being readily removable from the dispensing assembly to
permit quick simultaneous replacement of all the pipettes, or
simultaneous cleaning thereof in a device such as an autoclave.
A still further object of the invention resides in the provision of
a novel, vacuum-operated pneumatic control system for actuating the
dispensing assembly to simultaneously control the pickup and
dispensing of liquid from the plurality of pipettes and for
accurately and precisely determining the exact microquantities of
fluid which are to be picked up and dispensed.
Still other objects and advantages of the invention will become
more apparent from reading the following detailed description of
the invention as it proceeds with reference to the accompanying
drawings in which like numerals indicate like elements. However, it
is understood that the described embodiment is for illustrative
purposes only, and that the scope of the invention is to be
determined and limited solely by the appended claims.
BRIEF DESCRIPTION OF THE DRAWINGS
FIG. 1 is a general perspective view of the novel laboratory
dispensing apparatus of the invention;
FIG. 2 is a fragmentary, partially sectioned front elevation view
of the laboratory dispensing apparatus shown in FIG. 1 and
particularly illustrating the base construction of the
apparatus;
FIG. 3 is a fragmentary, partially sectioned view taken generally
along line 3--3 of FIG. 2 and illustrating particularly the manner
in which several of the electrical and pneumatic control components
and indicators and the liquid stirring device are mounted within
the base structure;
FIG. 4 is a fragmentary, partially sectioned plan view taken
generally along line 4--4 of FIG. 2 with the upper bed plate
removed and illustrating the general positions in which the various
electrical and pneumatic control components are mounted on the
lower base plate of the laboratory dispensing apparatus;
FIG. 5 is a top plan view of the liquid reservoir base and bottle
receiver forming a part of the invention;
FIG. 6 is a sectional elevation view taken generally along line
6--6 of FIG. 5;
FIG. 7 is an enlarged fragmentary, partially sectioned view of the
novel pickup and dispensing assembly which forms part of the
invention;
FIG. 8 is a fragmentary, partially sectioned side elevation view of
the dispenser unit shown in FIG. 7;
FIG. 9 is an enlarged fragmentary, partially sectioned view of an
individual pipette illustrating the manner in which it is mounted
within the dispensing assembly; and
FIG. 10 is a schematic diagram of the electrical and pneumatic
control systems by which the laboratory dispensing apparatus is
automatically operated.
DETAILED DESCRIPTION OF THE INVENTION
Referring now to the drawings, the novel automatic pickup and
dispensing apparatus 20 comprises a base assembly 22 having an
upper, Bakelite mounting bed-plate 24 covered by a thin decorative
brass plate 25, and a pickup and dispensing pipette assembly 26
which includes a plurality of pipettes 27. The assembly 26 is
mounted above bed-plate 24 via vertical post 28, control gear
housing 30, gear rack 32 mounted within housing 30 and driven by a
suitable pinion within housing 30 by handle 34, and L-bracket 36
fixed to the lower end of rack 32 and the rear end of dispensing
assembly 26 via screws 38. The pickup and dispensing assembly 26 is
moved vertically relative to the mounting bed-plate 24 by rotating
handle 34 to drive the rack 32 up and down.
A sample carrier plate 40 is slidably mounted within longitudinal
recesses provided in guide rails 42 and 44 which are spaced above
the bed-plate 24 by spacers 46 secured to the bed-plate. Carrier
plate 40 has a readily accessible knob 48 by which an operator may
slide the plate from its forward retracted position shown in FIG. 1
to a rearward operational position in which the plate underlies the
dispensing assembly 26.
Carrier plate 40 may support a plurality of separate vials or tubes
or may preferably support a transparent microtitration plate 50
having a plurality of wells 52 corresponding to the number of
pipettes 27 in the dispensing assembly 26. The microtitration plate
50 may be located on carrier 40 via a titration plate locator and
guide assembly (not shown) similar to that disclosed in application
Ser. No. 740,289. The use of such a locator assembly enables an
operator to readily replace one titration plate for another and
ensures that the wells 52 of each plate 50 will be accurately
aligned with their respective corresponding pipettes 27 during a
dispensing operation.
Mounted across the rear portion of mounting bed 24 is a clear
acrylic, liquid reservoir plate 54 (FIGS. 5 and 6) which is
positioned on bed-plate 24 by an end recess 56 that engages a guide
pin 58 extending upwardly from bed-plate 24 and by a locking pin 60
which extends downwardly through rail 44 into a blind hole 62
provided in the top surface of reservoir plate 54. Reservoir plate
54 has a cylindrical recess or well 64 communicating with a liquid
supply trough 66 located underneath the dispenser assembly 26 via a
pair of channels 68 and 70 arranged substantially tangentially to
the outer periphery of cylindrical recess 64 to provide for
recirculation of the liquid between recess 64 and trough 66 by an
agitating device described below. Another passage 72 extends
diagonally between the supply passage 68 and trough 66 to ensure
that the liquid at the center of trough 66 remains in an agitated
state. A liquid reservoir tank or bottle 74 is mounted in an
inverted state above cylindrical recess 64 via bottle receiver 76
having a central opening 77, the receiver 76 being fixed to the
upper surface of reservoir plate 54 by screws 78.
A permanent magnet bar stirrer 80 is supported on the bottom of
recess 64 via a non-friction bearing stud 82 and is rotated by the
magnetic drive assembly 90 (FIGS. 2-4) to circulate the liquid from
recess 64 through channel 68 into trough 66 and then back through
channel 70 to recess 64. A portion of the liquid from channel 68
passes through channel 72 toward the center of trough 66 to ensure
that all the liquid in trough 66 remains agitated and prevents any
occurrence of dead spots within the trough.
Referring now particularly to FIGS. 2-4, the base assembly 22 also
includes a lower base plate 84 supported from a table or other
suitable support by mounting pads 86, with the mounting bed-plate
24 being spaced above base plate 84 and supported thereon by
vertical posts 88. The various electrical and pneumatic control
components, which will be described later with respect to a typical
dispensing operation, and the agitator magnetic drive assembly 90
are supported on base plate 84 within the confines of an annular
shroud or cover member 92 connected at its upper end to the
peripheral edge of mounting bed 24.
Mounting bed 24 has an elongated slotted opening 93 estending along
one side thereof. The agitator drive assembly 90 includes a motor
94 mounted on base plate 84 for rotating a suitable holder for a
permanent bar magnet 96 which is positioned within slot 92 under
the brass plate 25 directly beneath the cylindrical recess 64 of
reservoir plate 54. As the drive magnet 96 is rotated, stirrer
magnet 80 in recess 64 will follow magnet 96 and will thereby
circulate the fluid between recess 64 and through 66 to ensure that
the liquid, for example a saline solution, will be of uniform and
homogeneous composition at all times.
Referring now particularly to FIGS. 1 and 7-9, the dispensing
assembly 26 includes an upper manifold plate 100, a piston guide
plate 102 secured to manifold 100 via screws 104, and a removable
dropper or pipette head unit 106 which includes a dropper plate 108
and a dropper spring retainer plate 110 secured to plate 108 via
screws 112. The dropper head unit 106 which supports all the
pipettes 27 as a removable, self-contained unit is removably
clamped to manifold 100 and guide plate 102 by a pair of side
clamps 114 and 116, each of which has a horizontal lip 118 engaging
the bottom side edges of retainer plate 110. As shown best in FIGS.
1 and 7, a pair of adjusting jack screws 120 is connected to each
clamp 114 and 116. Each jack screw has an upper, large-diameter
cylindrical portion 122 which is slidable within an opening 124 in
manifold 100 and a reduced diameter portion 126 which extends
through a complimentary hold in guide plate 102, with the lower end
of section 126 being threaded through a mating threaded opening in
clamp 114 or 116. A compressed spring 128 surrounds screw section
126 of each of the adjusting screws in position between the opposed
faces of guide plate 102 and clamps 114 and 116. A locking cam 130
is pivotally supported between the upper ends of each cooperating
pair of jack screws 120 on a pin 132 which extends between the
screws and is retained thereon by snap rings 134. A washer 136
surrounds each of the screws 120 in resting engagement with the top
surface of manifold 100, and each of the locking cams 130 includes
a locking cam surface 138 which, when engaged with washer 136 as
shown in FIG. 7, fastens the dropper unit 106 in operative relation
to manifold 100 and guide plate 102. When the locking cams 130 are
pivoted to an upright position, jack screws 120 will slide
downwardly relative to manifold 100 and plate 102 and thereby cause
the clamps 114 and 116 and dropper unit 106 to be lowered to a
release position (shown in phantom lines in FIG. 7) in which the
entire unit 106 may be removed simply by sliding the unit forwardly
over the retaining lips 118. The dropper unit 106 is properly
positioned in place during operation of the unit by abutment of the
rear ends of plates 108 and 110 against the bottom edge of the
support bracket 36 as shown best in FIG. 8.
The dispenser assembly is operated by a vacuum source and, to
accomplish this, the manifold 100 includes a horizontal passageway
140 extending through one side face of the manifold and a vertical
passageway 142 intersecting and depending downwardly from passage
140 through the bottom face of manifold 100. The outer end of
passage 140 is connected to a vacuum source by way of conduit 144
and a suitable threaded coupling 146.
Piston plate 102 has an uppermost rectangular annular recess 148
which receives a sealing gasket 150 providing a seal between the
opposed faces of manifold 100 and plate 102. Plate 102 also has an
enlarged central rectangular recess 152 communicating with passage
142, with recess 152 being intersected by a plurality of bores 154
which open through the bottom face of plate 102. In the embodiment
illustrated, there are 96 bores 154 corresponding in number to the
96 pipettes 27.
A piston 156 is slidably received in each of bores 154 and is
biased downwardly by a spring 158. A thin flexible diaphragm 160 is
positioned between the opposing faces of the piston guide plate 102
and the dropper plate 108 when the dropper assembly 106 is
positively fastened to plate 102. In such a position, diaphragm 160
closes the bottom ends of bores 154 and thereby separates bores 154
from aligned openings 162 provided through the upper face of
dropper plate 108 which also has an enlarged rectangular bottom
recess 164. The dropper spring retainer plate 110 closes the bottom
of recess 164 and itself includes a plurality of openings 166 which
are aligned with openings 162 and bores 154.
Each of the actuating pistons and the corresponding pipettes is
identical to that illustrated in FIG. 9 and therefore only one
piston and its actuating effect on its associated pipette 27 will
be described. The pistons 156 include an upper annular flange
section 168 adapted to seat on the bottom of recess 152 around bore
154. A reduced diameter piston section 170 is loosely received
within bore 154 to provide a clearance therebetween and spring 158
is received within an axial blind bore 172 to normally bias the
piston 156 downwardly so that its bottom end firmly engages the
upper side of the diaphragm 160.
Each pipette 27 includes a large tubular section 174 having at its
upper end an enlarged diameter section 176 which is slidable but
closely fitted within opening 162 of dropper plate 108. The upper
end of section 176 is formed with an annular flange 178 which
defines an upper recess 180 communicating with the central
passageway 182 of tubular section 174. The lower end of section 176
is formed with a shoulder 184, and a compressed spring 186 acts
between shoulder 184 and the upper face of the spring retainer
plate 112 to bias the dropper 27 upwardly so that the annular
flange 178 sealingly engages the underside of diaphragm 160. The
lower end of tubular section 174 extends outwardly through opening
166 of plate 110 and has a reduced diameter tubular stem 188
press-fitted within its bottom end in flow communication with the
passage 182.
In operation, when a vacuum is applied through passages 140 and 142
to recess 152, piston 168 will be moved upwardly within bore 154
and the vacuum will be applied to the top of diaphragm 160, which
then will be retracted within bore 154. As a result, a suction
effect will be created within recess 180 and passage 182 of pipette
27 and, if the bottom end of the pipette stem 188 is immersed in
the liquid in trough 66, a predetermined quantity of the liquid
will be sucked into the pipette.
The actuating pressure acting on diaphragm 160, in addition to
being set by the pneumatic control components described
hereinbelow, is determined by properly adjusting jack screws 120 to
set the clamping force applied by claims 114 and 116 to hold the
pipette head unit 106 against diaphragm 160 and plate 102. For
example, jack screws 120 may be adjusted to provide approximately 7
to 10 pounds actuating pressure with the head unit in place.
It should be especially noted that each of the pipettes 27 is
permanently mounted within the dropper unit 106 so long as the
plates 108 and 110 are maintained in assembled relationship. When
unit 106 is to be removed from manifold 100 and guide plate 102 by
releasing the locking cams 130 and clamps 114 and 116, the annular
shoulder 184 of each of the pipettes will engage the wall surfaces
of dropper plate 108 surrounding the openings 162 and thereby
maintain the pipettes in assembled relationship between plates 108
and 110. Consequently, the entire dropper unit 106 may be removed
and placed in a device such as an autoclave for simultaneously
cleaning all the pipettes together without having to disturb the
individual mounting or assemblage of each individual pipette.
A typical pickup and dispensing operation will now be described
with special reference to FIGS. 1 to 4 and 10, with FIG. 4
generally illustrating the position of the various electrical and
pneumatic control components as they are mounted on the base plate
84 enclosed within shroud 92, and FIG. 10 schematically
illustrating the electrical and pneumatic connections to the
various components. Flexible conduit 144 extends from manifold 100
downwardly through suitable openings in the decorative coverplate
25 and mounting bed 24 within shroud 92 and is connected to a
solenoid operated valve 188. Valve 188 is connected to a suitable
vacuum source via vacuum gauge 190 and conduit 192 which has a
coupling end 194 extending outwardly through a slot 196 provided in
the rear wall of annular shroud 92. The head of vacuum gauge 190 is
positioned so as to lie within the elongated slot 93 of mounting
bed 24 and decorative plate 25 has a suitable opening and
protective transparent cover through which the gauge 190 may be
read by an operator as illustrated in FIG. 1. It should be noted
that the valve 188, gauge 190, and connecting conduits are all
supported on base plate 84 within the annular shroud 92.
The electrical operating circuit for the dispensing apparatus
includes an on-off switch 196 and indicator light 198 mounted on
the top of decorative plate 25 and a foot operated switch 200
conveniently located for use by an operator. The main electrical
system of the apparatus includes a terminal board 202 having
contacts C1, C2, C3, C4, C5 and C6, with terminal board 202 being
mounted on the top of base plate 84. A three-wire electrical
conductor 204 has its ground lead connected to contacts C1 and its
other leads 208 and 210 connected to contacts C2 and C3,
respectively. The electrical system also includes a transformer 212
for reducing the supply voltage of either 110 volts or 220 volts to
a predetermined control voltage used to operate the agitating motor
94 and the other components of the control system. The transformer
212 has an output terminal board 214 having contacts A, B and C,
with contact B being a ground terminal connected to ground contact
C1 of terminal board 202 by a conductor 216 and to a machine ground
218 by a conductor 220.
To use the apparatus, an operator will initially close switch 196
to pass current from contact C2 through lines 222 and 224, fuse
226, line 228, the appropriate contacts of a slide switch 230 to
the primary side T1 of transformer 212. Slide switch 230 is of a
conventional type by which either 110 volts or 220 volts can be
supplied to the primary side T1 of transformer 212. Current will
then be supplied from the secondary side T2 of transformer 212 to
the output terminals A, B and C of terminal board 214. Current then
flows from terminal C of board 214 to the agitating drive motor 94
via conductor 232, contacts C4 of board 202, conductor 236 through
motor 94 to conductor 238, resistor 240, potentiometer 242, and
conductor 244 back to contact C5 of board 202. The speed of motor
94 may be adjusted by proper adjustment of potentiometer 242 to
vary the speed of rotation of drive magnet 96 and follower magnet
80 within recess 64. In this way, the rate of circulation of the
liquid in reservoir 54 may be properly adjusted.
Following closure of switch 196, the indicator lamp 198 will be
lighted as current flows from contact C4 through the lamp and back
to contact C5 of board 202 and contact A of board 214 via conductor
246. Thus, the operator is informed of proper operation of the
system by the lighting of lamp 198.
With the agitating drive system 90 continually operating and the
liquid being circulated between recess 64 and trough 66 to ensure
uniformity of the characteristics of the liquid, the operator may
then effect a dispensing operation as follows. With the carrier 40
and microtitration plate 50 in its inoperative retracted position
shown in FIG. 1, the dispensing assembly 26 may be lowered by
rotation of handle 34 until the open ends of pipettes 27 are
immersed in the liquid in trough 66. The operator then depresses
the foot operated switch 200 which causes current to flow from
contact C4 through the closed switch 200 to contact C6, through
conductor 248 to the operating solenoid 250 of valve 188, and back
to contact C5 via conductor 252. Energization of solenoid 250 will
open valve 188 to thereby connect conduit 144 to the vacuum in
conduit 192.
This vacuum will then be applied through the passages 140 and 142
of manifold 100 to the enlarged guide plate recess 152 which acts
as a vacuum chamber. Pistons 156 will be raised within their
respective bores 154 and the vacuum will then be applied to the top
of diaphragm 160 which will be retracted within the bores 154.
Consequently, a suction will be created within each of the pipettes
27 and a predetermined amount of liquid will be withdrawn into the
tubular passageways of each pipette. While still holding the foot
operated switch 200 depressed to continue application of the vacuum
to manifold 100, the operator will then raise the dispensing
assembly 26 out of trough 66 and thereafter slide carrier plate 40
along guide tracks 42 and 44 to its rearward operative position in
which it overlies trough 66 and in which the wells 52 of
microtitration plate 50 are accurately aligned with respective ones
of pipettes 27. Dispensing assembly 26 is again lowered so that
each of the pipettes 27 is positioned within an aligned well 52 of
plate 50. The operator then releases foot-operated switch 200 to
deenergize solenoid 250 and close valve 188 to disconnect conduit
144 from conduit 192. Valve 188 includes a conventional means (not
shown) which, when the valve is in the closed position, will
automatically vent conduit 144 to atmosphere. Thus, when valve 188
is closed, atmospheric pressure will again be applied to manifold
100 through conduit 144 to thereby cause pistons 156 and diaphragm
160 to be biased downwardly by springs 158. Downward movement of
diaphragm 160 will cause the liquid in each of the pipettes 27 to
be simultaneously expelled from the pipettes into wells 52 of
microtitration plate 50.
The dispensing assembly 26 is then raised to its uppermost position
and carrier 40 returned to its forward retracted position in which
microtitration plate 50 may be readily removed and another plate
placed on the carrier 40 for the next dispensing operation.
From the above description, it is apparent that the laboratory
dispensing apparatus of the invention accomplishes the objects and
provides the advantages initially set forth by providing a device
which is highly reliable and accurate in simultaneously dispensing
reproduceable microquantities of liquid; is compact and
self-contained and therefore readily portable and manipulatable; is
adapted for usage with various type liquids including those having
several constituents; and is easily cleaned when necessary due to
the fact that the dispenser head unit supporting the plural number
of pipettes is quickly and easily removed from the apparatus for
placement in a device such as an autoclave.
It is understood of course, that various other modifications and
embodiments are encompassed within the scope of the invention
which, of course, is not limited by the detailed description above.
For example, when used with some liquids, it may be necessary to
provide a suitable coating on the external surfaces of the pipettes
to prevent any adherence of the liquid thereto which might
adversely affect the accuracy of the microquantities of liquid
being dispensed.
The invention may be embodied in other specific forms without
departing from the spirit or essential characteristics thereof. The
present embodiment is therefore to be considered in all respects as
illustrative and not restrictive, the scope of the invention being
indicated by the appended claims rather than by the foregoing
description, and all changes which come within the meaning and
range of equivalency of the claims are therefore intended to be
embraced therein.
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