U.S. patent number 3,615,241 [Application Number 05/015,022] was granted by the patent office on 1971-10-26 for firefly pump-metering system.
Invention is credited to George M. Low, N/A, Chris J. Plakas.
United States Patent |
3,615,241 |
Low , et al. |
October 26, 1971 |
FIREFLY PUMP-METERING SYSTEM
Abstract
A pumping and metering dual piston system for simultaneously
injecting controlled amounts of sample and reactants into a
reaction chamber and an enzyme into an adjacent injection chamber.
A rotary shaft is actuated to communicate the injection chamber
with the reaction chamber and to actuate a mechanism for forcing
the enzyme from the injection chamber to the reaction chamber.
Additionally, the shaft supports and operatively rotates detector
apparatus from a magnetic and light radiation shielded position to
a position for monitoring the reaction chamber constituents.
Inventors: |
Low; George M. (N/A),
N/A (N/A), Plakas; Chris J. |
Family
ID: |
21769120 |
Appl.
No.: |
05/015,022 |
Filed: |
February 27, 1970 |
Current U.S.
Class: |
422/81; 141/23;
222/135; 222/309; 422/52; 435/286.4; 435/287.3 |
Current CPC
Class: |
G01N
1/38 (20130101) |
Current International
Class: |
G01N
1/38 (20060101); G01N 001/14 (); B67D 005/16 () |
Field of
Search: |
;23/259,253 ;73/425.6
;222/71,135,309 ;141/23 ;195/127 |
References Cited
[Referenced By]
U.S. Patent Documents
Primary Examiner: Wolk; Morris O.
Assistant Examiner: Serwin; R. E.
Claims
It is claimed:
1. A pumping and metering system comprising:
means for storing a sample to be analyzed;
means for storing a reagent separately from said sample;
a housing defining a reaction chamber in communication with said
sample-storing means and an injection chamber in communication with
said reagent storing means;
metering means for adjustably controlling the volume of said
reaction chamber and said injection chamber, said metering means
including a first piston slidable within said housing and mounted
on an elongated shaft thereby defining said injection chamber and a
second slidable piston mounted on said shaft within said housing
thereby defining said reaction chamber;
adjustment means to control the volume of said reaction chamber
including a nut threadably adjustable on said shaft and providing a
stop in order to limit sliding travel of said second piston;
initially closed valve means providing communication between said
reaction and said injection chambers;
pumping means for transferring reagent from said injection chamber
to said reaction chamber; and,
automatically actuable means for actuating said valve means and
said pumping means in a properly determined timed sequence.
2. The structure of claim 1, wherein said automatically actuable
means comprises a rotatable shaft and said valve means comprises a
groove conduit in said shaft.
3. The structure of claim 1, and further including a radiation
shielded housing containing a pivotally mounted detector means,
said automatic actuable means being so constructed and arranged to
open said valve means, activate said pumping means and pivot said
detector means into registration with said reaction chamber.
4. The structure of claim 1, wherein said pumping means includes a
coil spring surrounding said shaft, a spring retainer slidably
receiving said shaft and retaining said coil spring initially in
compression, latching means initially precluding motion of said
retainer means, said automatically actuable means adapted to
release said latching means permitting expansion of said coil
spring for transferring said reagent, said adjustment nut adapted
for engagement on said spring retainer.
5. The structure of claim 1 and further including a first
distribution manifold providing communication between said
sample-storing means and said reaction chamber and a second
distribution manifold providing communication between said
reagent-storing means and said injection chamber.
6. The structure of claim 5 and further including an automatically
actuable distribution valve operatively associated with said
sample-storing means and said reagent-storing means for
simultaneously supplying sample and reagent to said manifolds.
Description
The invention described herein was made in the performance of work
under a NASA contract and is subject to the provisions of section
305 of the National Aeronautics and Space Act of 1958, Public Law
85-568 (72 Stat. 435; 42 USC 2457).
The present invention relates to a pumping and metering system for
laboratory sample analysis, and, more particularly, to an
instrument for automatically mixing a sample with reactants and for
injecting controlled amount of the same into a reaction chamber.
Simultaneously, an enzyme or other reagent is metered into an
adjacent injection chamber. A rotatable shaft is then actuated to
operatively connect the reaction and injection chambers and to
actuate a mechanism for forcing the reagent from the injection
chamber into the reaction chamber. The shaft further is actuated to
operatively position detector apparatus in registration with the
reaction chamber for monitoring the constituents therein.
BACKGROUND OF THE PRIOR ART
A typical pumping and metering system of the prior art is described
in U.S. Pat. No. 3,193,358 wherein a reciprocable piston divides a
surrounding housing into two reacting chambers. An adjusting screw
is utilized to limit piston travel enabling the dispensing of a
measured portion of a mixed sample and reagent. A disadvantage of
such construction resides in a requirement for separate sample and
reagent mixing chambers and the absence of an automatic control for
simultaneously mixing the sample and reagent and positioning
detector apparatus for monitoring the mixed constituents.
BRIEF SUMMARY OF THE INVENTION
Apparatus according to the invention provides a storage unit for
separately containing a sample, reactants and an enzyme or other
reagent. A solenoid valve is activated to mix the sample and
reactants and inject the same into a reaction chamber.
Simultaneously the solenoid valve permits injection of the reagent
into an injection chamber. Volume of the injection chamber is
controlled by a reciprocating piston, the travel of which is
purposely preadjusted. Volume of the reaction chamber is purposely
metered by controlled travel of a second floating piston. As the
injection and reaction chambers receive the reagent and sample, the
two pistons will be caused to stop in abutting engagement with each
other thereby metering controlled amounts of reagent and sample
within the respective chambers. A rotatable shaft is then
automatically actuated to operatively communicate the injection
chamber with the reaction chamber. Further, actuation of the
rotatable shaft releases a spring-loaded mechanism for slidably
forcing the first piston to compress the reagent and force the same
from the injection chamber into the reaction chamber. Additionally,
rotation of the shaft rotatably positions a photomultiplier or
other detector apparatus into registration with the reaction
chamber in order to monitor the constituents therein.
OBJECTS OF THE INVENTION
An object of the present invention is to provide apparatus for
pumping and metering controlled amounts of sample, reactants and
reagents into adjacent controlled volume chambers, and for
automatically mixing the sample and reagent within one of the
chambers, and operatively positioning a detector apparatus for
monitoring the constituents of said chamber.
Another object of the present invention is to provide apparatus for
automatically pumping and metering controlled amounts of sample to
a reaction chamber and controlled amounts of reactant to an
injection chamber, and further for simultaneously automatically
mixing the sample and the reactant and positioning detector
apparatus which monitors the mixed constituents.
A further object of the invention is to provide a reactant
injection chamber defined by a first controlled travel piston, a
sample-receiving reaction chamber, the volume of which is
determined by a floating second piston engageably stopped on said
first piston, an automatic mechanism for reciprocating said first
piston in order to transfer the reagent from the injection chamber
to the reaction chamber.
Another object of the present invention is to provide a reaction
chamber and injection chamber each defined by a reciprocating
piston, one of the pistons metering a controlled amount of sample
injected into the reaction chamber and the other piston both
metering a controlled amount of reagent supplied to the injection
chamber and pumping the reagent into the reaction chamber.
Other objects and many attendant advantages of the present
invention will become obvious upon perusal of the following
detailed description taken in conjunction with the accompanying
drawing.
BRIEF DESCRIPTION OF THE DRAWING
The FIGURE is a schematic in elevation and partially in section
illustrating a preferred embodiment of the pumping and metering
system according to the present invention.
DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENT
With more particular reference to the drawing, there is generally
illustrated in the FIGURE at 10 a storage and distribution unit
comprising a housing 12 provided with three cylindrical vertically
oriented storage cavities 14, 16 and 18. The storage cavity 14 is
provided with a cover plate 20 having attached thereto an inverted
depending retaining collar 22 having attached thereto one end of a
compression coil spring 24 extending axially vertically within the
storage cavity 14. The other end of the coil spring 24 is secured
to a reciprocating piston 26 provided with a circumferentially
surrounding ring seal 28. Similarly, the cavities 16 and 18 are
provided with cover plates and structure similar to that described
in conjunction with the cavity 14. However, such structure will not
be described in detail for clarity. The bottom portions of cavities
14 and 16 are connected by a reduced diameter passageway 30 having
a plug valve or other suitable valve 32 interposed therein. The
bottoms of cavities 16 and 18 are provided with vertically oriented
outlet passageways 34 and 36, respectively. The passageways 34 and
36 extend through and communicate with a laterally extending,
enlarged diameter bore 38. Slidably reciprocable within the bore 38
is an elongated armature 40 of a solenoid 42 mounted on a lateral
surface of the housing 12. The armature 40, shown in its retracted
position, is provided with longitudinally spaced reduced diameter
portions 44 and 46 which portions, will register in line with the
passageways 34 and 36, respectively, when the armature 40 is in its
extended position. Accordingly, in such extended position, the
armature 40 acts as an automatically actuated double distribution
valve. The passageways 34 and 36 are connected to distribution
conduits 48 and 50, respectively. The conduit 48 operatively
supplies a four-outlet distribution manifold indicated
schematically at 52. Similarly, the conduit 50 supplies a
distribution manifold 54 similar in construction to the manifold
52.
In the FIGURE, there is illustrated generally at 56 a fragmentary
schematic of an injection unit 56 provided at its lowermost portion
with an aluminum chamber block 58, only a portion of which is
illustrated in the fragmentary view. The chamber block 58 is
provided with a vertically extending bore 60 within which is
received an elongated rotary shaft 62. The top portion of the
chamber block 58 is provided with an enlarged diameter recess 64
laterally offset from the bore 60. The recess 64 opens into a
generally reduced diameter bore 66 coaxial with the recess 64
extending vertically parallel to the rotary shaft 62. In practice,
the rotary shaft 62 is disposed centrally of the chamber block 58
and four laterally offset bores 66 are provided. However, for
clarity, only one of such bores will be described in detail
hereinafter.
The recess 64 receives therein a transparent glass window 68 which
forms a sealing cover plate for the bore 66. A laterally extending
intake passageway 70 extends through the chamber block 58 into the
bore 66 immediately adjacent to and under the bottom surface of the
window 68. The passageway 70 is provided with a shutoff valve 72 to
which is connected one outlet 74 from the distribution manifold 52.
In similar fashion, an inlet passageway 76 is provided through the
chamber block 58 adjacent the bottom wall of the bore 66. The
passageway 76 communicates with a shutoff valve 78 to which is
operatively connected one of the outlet conduits 80 of the
distribution manifold 54.
Axially aligned with the bore 66 is an inverted recess 82 provided
in the chamber block 58. A generally centrally located opening
connects the recess 82 with the bottom portion of the bore 66 and
has mounted therein a generally annular sealing ring 84 which
surroundingly and slidably receives a reciprocating piston shaft
86. At one end of the piston shaft 86 is secured a generally
cylindrical piston 88 disposed within the bottom portion of the
bore 66. The piston 88 carries a circumferentially surrounding
sealing ring 90 which sealably isolates the bottom portion of the
bore 66 from its remainder thereof to define a reagent injection
chamber 92. Accordingly, the injection chamber 92 is supplied
through the conduit 76, the shutoff valve 78 and the outlet conduit
80 of the distribution manifold 54. In the remaining portion of the
bore 66 is disposed a floating piston 94 carrying a surrounding
annular sealing ring 96. The floating piston 94 carries a depending
adjusting screw 98 having a flat head provided purposely to stop
against the piston 88 in a manner to be hereinafter described in
detail. Accordingly, the piston 94 sealably isolates the top
portion of the bore 66 and defines thereby a sample reaction
chamber 100 which is supplied by the conduit 70, the shutoff valve
72 and the outlet conduit 74 of the distribution manifold 52. It
should be noted, that the remaining not shown bores 66 are provided
with similar structure and are supplied in similar fashion by the
remaining outlet conduits from the manifolds 52 and 54. A detailed
description of the same is omitted for purposes of clarity.
With more particular reference yet to the FIGURE, the reciprocating
piston shaft 86 is slidably extended through the inverted recess 82
and is provided with a surrounding concentric injection compression
spring 102 retained initially in compression against the bottom
wall of the recess 82 by an impinging injection spring retainer 104
slidably received in the recess 82 but initially restrained against
movement by a ball latch 106 partially protruding into the recess
82 and engaged in a groove 108 provided circumferentially of the
injection spring retainer 104. The reciprocating piston shaft 86 is
slidably received centrally of the injection spring retainer 104.
The end portion of the reciprocating piston shaft 86 is threadably
provided thereover with a volume adjustment nut 110 initially in
spaced relationship from the injection spring retainer 104.
Initially, the ball latch 106 is retained in registration with the
injection spring retainer 104 by a ball release mechanism
illustrated schematically at 112. The ball release mechanism 112 is
rigidly secured to and is adapted for rotation by the rotary shaft
62 to position a recess 113 as hereinafter described. A motor 114
is operatively connected to the rotary shaft 62. In its generally
central portion the rotary shaft 62 is provided with a reduced
diameter conduit groove 116 which extends generally parallel to the
central axis of the shaft. The reagent injection chamber 92 is
provided with an outlet passageway 118 and the sample reaction
chamber 100 is provided with a similar reduced diameter passageway
120. Each of the passageways 118 and 120 communicate with the
groove conduit 116 of the rotary shaft 62. It should be understood,
however, that such communication occurs only upon rotation of the
rotary shaft to be hereinafter described in detail. Accordingly,
the initial position of the groove conduit and the recess is
illustrated in phantom line at 116' and 113' so that initially no
communication between the reagent injection chamber 92 and the
sample reaction chamber 100 can occur. The remaining end of the
rotary shaft 62 is operatively connected with a photomultiplier or
other suitable detector mounted within a magnetic casing 122. For
added support, the casing 122 may be carried by a mounting tray
124.
Casing 122 may be carried by a mounting tray 124 secured to the top
portion of the chamber block 58. For further protection, the casing
122 may be contained within an inverted light shielding housing 126
also secured to the top portion of the chamber block 58 by any
well-known fabrication techniques.
In operation, a quantity of sample to be tested is deposited within
the cavity 16 of the storage and distribution unit 10.
Additionally, a quantity of another substance, such as ATP
extracts, is deposited within the cavity 14. Also, a reagent or a
quantity of any enzyme may be deposited in the cavity 18. Each
cavity is then covered with its appropriate cover plate 20. The
depending spring 24 of each cover plate will be compressed due to
the quantity of substance within each cavity. Accordingly, each
piston 28 will exert pressure upon the quantity of substance within
each storage cavity. Subsequently, the plug valve 32 is opened to
permit communication between the cavities 14 and 16, for example,
to mix the sample with the ATP extracts. Simultaneously, the
solenoid 42 is actuated, thereby extending its armature 40 and
aligning the reduced diameter portions 44 and 46 thereof with the
outlet conduits 34 and 36. Ad a result of the solenoid actuation,
the mixed substances within the sample cavity 16 and the enzyme
substance within the cavity 18 will simultaneously be supplied
under pressure provided by the pistons 26 of the storage cavities
16 and 18 to the distribution manifolds 52 and 54. Enzyme will be
supplied under such pressure through the check valve 78 into the
reagent injection chamber 92. Such pressure additionally
reciprocates the piston 88 in order to accommodate the increasing
volume of enzyme supplied to the injection chamber 92. The piston
will continue to rise until the volume adjustment nut 110 impinges
against the injection spring retainer 104. Accordingly, the volume
of enzyme accepted by the injection chamber 92 is positively
controlled by proper adjustment of the volume adjustment nut 110.
Simultaneously, the mixed sample and ATP extracts from the manifold
52 are supplied through the check valve 72 into the sample reaction
chamber 100. The floating piston 94 will reciprocate in response to
the expanding volume of sample accepted into the reaction chamber
100 until the adjustment screw 98 stops against the piston 88.
Accordingly, the volume of sample accepted into the reaction
chamber 100 can be adjustably controlled by a proper setting of the
adjustment screw 98.
At this point in the operation the valves 72 and 78 are closed
thereby isolating the injection chamber 92 and the reaction chamber
100. The motor 114 is then actuated to operatively rotate the
rotary shaft 62. By such operation, the groove conduit 116 is
brought into registration with the passageways 118 and 120 of the
injection chamber 92 and the reaction chamber chamber 100.
Simultaneously, the ball release mechanism 112 is rotated to bring
the recess 113 in registration with the ball latch 106, permitting
the same to be withdrawn from the groove 108 of the injection
spring retainer 104. Accordingly, the compressed coil spring 102 is
permitted to expand, thereby reciprocating the piston 88 and
purging the injection chamber 92 of the enzyme which is caused to
flow through the passageway 118, the conduit 116, the passageway
120 and into the sample reaction chamber 100. Such reciprocation of
the piston 88 permits a corresponding reciprocation of the piston
94, permitting expansion of the reaction chamber 100 to accommodate
the increase in volume thereof due to the enzyme received therein.
Additionally, rotation of the rotary shaft 62 rotates
photomultiplier (not shown) initially from a position within the
magnetic shield casing 122 to a position in registration with the
glass window 68 covering the reaction chamber 100. Accordingly, the
photomultiplier is operatively positioned upon rotation of the
rotary shaft 62 to monitor the mixed constituents within the
reaction chamber 100.
Accordingly, and in accordance with the objects of the present
invention, the preferred embodiment of the invention is a dual
piston sample analysis instrument which both meters the amount of
the reactants and pumps the same into the reaction chamber.
Additionally, the present invention utilizes an uncomplicated
mechanism in the form of a rotary shaft for accomplishing within
significant time relationships, three functions: namely, releasing
a ball detent latch and enzyme injection spring, activating a
conduit permitting enzyme to flow from an injection chamber into a
reaction chamber where it is mixed with a sample to be analyzed,
and supporting and positioning a photomultiplier tube from a
protected position to a position whereby monitoring of the mixed
constituents is accomplished. Other embodiments and modifications
of the present invention are apparent and intended to be protected
by the scope of the appended claims.
* * * * *