U.S. patent number 3,593,909 [Application Number 04/820,232] was granted by the patent office on 1971-07-20 for reaction vessel closure.
This patent grant is currently assigned to Eppendorf Geraetebau Netheler & Hinz G.m.b.H.. Invention is credited to Wilhelm Bergmann.
United States Patent |
3,593,909 |
Bergmann |
July 20, 1971 |
REACTION VESSEL CLOSURE
Abstract
A vessel for small quantities of liquid including a top closure
comprising a pierceable foil responsive to puncture by an obliquely
sharpened cannula by elastic deformation of the prick hole into
close bearing contact with the cannula.
Inventors: |
Bergmann; Wilhelm (Hamburg,
DT) |
Assignee: |
Eppendorf Geraetebau Netheler &
Hinz G.m.b.H. (Hamburg, DT)
|
Family
ID: |
5701644 |
Appl.
No.: |
04/820,232 |
Filed: |
April 29, 1969 |
Foreign Application Priority Data
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May 2, 1968 [DT] |
|
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P 17 73 331.2 |
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Current U.S.
Class: |
220/277; 220/375;
220/359.4; 422/913; 215/247 |
Current CPC
Class: |
B01L
3/5082 (20130101) |
Current International
Class: |
B01L
3/14 (20060101); B65d 005/64 (); B65d 043/00 () |
Field of
Search: |
;229/43
;215/37,38,46,47,DIG.3 ;128/272 ;220/27,38.5 |
References Cited
[Referenced By]
U.S. Patent Documents
Primary Examiner: Hall; George T.
Claims
What I claim is:
1. A reaction vessel for small quantities of liquid and having a
top closure adapted to be pierced by a sharpened cannula, said top
closure consisting of a foil bridging substantially the entire
throat of said vessel, said foil exhibiting sufficient elasticity
to provide elastic deformation of a prick hole edge to effect
bearing contact upon the outer wall of such a cannula.
2. A reaction vessel according to claim 1, characterized in that
said foil has a thickness within the range of 0.08 to 0.35 mm.
3. A reaction vessel according to claim 1 wherein said foil is
joined to the upper vessel edge by welding.
4. A reaction vessel according to claim 3 wherein said foil is
applied under a slight tension.
5. A reaction vessel according to claim 3 wherein the thickness of
said foil is in the range of 0.08 to 0.2 mm.
6. A reaction vessel according to claim 3 wherein said foil is
approximately 0.1 mm. thick.
7. A reaction vessel according to claim 1 wherein said foil is an
integral part of a removable cover and said cover includes a
reinforced edge, said cover being adapted for insertion into the
upper throat of the vessel.
8. A reaction vessel according to claim 7 wherein the thickness of
the foil is in the range of 0.2 to 0.3 mm.
9. A reaction vessel according to claim 1 wherein the foil and the
vessel are both fabricated from a polypropylene-based plastic.
10. A reaction vessel according to claim 7, wherein said cover and
said vessel are formed as integral components of a single
polypropylene-based plastic unit.
11. A reaction vessel for small quantities of liquid, having a
substantially cylindrical upper body portion with a top closure
adapted to be pierced by a sharpened cannula; said top closure
consisting of a foil substantially bridging the opening of said
upper body portion; said foil and said vessel being fabricated of
polypropylene-based plastic; and said foil having a thickness of
0.08 to 0.35 mm. and being characterized by sufficient elasticity
such that the prick hole edge effects bearing contact upon the
outer wall of a piercing cannula.
12. A reaction vessel according to claim 11, wherein said foil is
an integral part of a removable cover having a reinforced edge and
dimensioned to fit within the opening of said upper body portion,
and wherein said cover is an integral part of said vessel.
Description
BACKGROUND OF THE INVENTION
The invention relates to reaction vessels for holding small
quantities of liquids. In particular, this invention relates to
reaction vessels which may be handled automatically and which
include top closures.
It is common to use small vessels for the processing of small
quantities of liquids. During such processing, for example,
portions of the liquid within the reaction vessel may be extracted
or inserted with a pipette, or cannula. Generally, test tubes are
not suitable because due to their dimensions they tend to spread
out the small quantities of liquid, they are cumbersome to clean,
and they are relatively expensive.
On the other hand, it has also become common to process small
quantities of liquids while using small plastic reaction vessels.
Such vessels, for example, may be about 30 mm. high and have a
diameter of 15 mm. These vessels are cheap to produce and need not
be cleaned since they are dispensable. This is advantageous because
it saves the cost of cleaning and also eliminates possible faulty
reactions due to deficient cleaning.
It is important to provide a closure for the reaction vessel that
can be easily handled, particularly during the processing of the
liquid and still more particularly, in the event that the
processing is automatic. During automatic processing, a portion of
the liquid is often taken out by means of a cannula introduced into
the vessel. In the event of a series of tests, the cannula may be
brought successively into different vessels, each containing
different liquids. The cannula is customarily cleaned internally
between successive operations. On the other hand, there is a danger
during such series tests that errors will be introduced due to the
entrainment of liquid on the outside of the cannula.
In the past, test tubes and reaction vessels have been sealed with
cotton wads or elastic covers such as rubber stoppers. It is
particularly undesirable to use cotton wads with reaction vessels
because they do not make a tight seal and they will often foul the
stored liquid. These prior sealing means offer no solution to the
problem of entrainment of liquids on the outside of the cannulas
and they prove a nuisance when they must be completely opened
before the introduction of the cannula.
A still further problem encountered when using reaction vessels
arises because such vessels are sometimes heated. Due to the
resulting volume expansion of the contents of the vessel, if the
vessel cover is clamped closed, there is a danger that the cover
will be forcibly ejected. In the event of this occurrence, the
vessel closure is completely removed and creates the possibility of
vaporization or evaporation of the contents. In fact, during
certain processes, even the temporary removal of a cover gives rise
to this possible disadvantage.
SUMMARY OF THE INVENTION
The problems noted above are solved by the unique combination of
vessel and closure means utilized in the present invention. The
reaction vessel disclosed herein provides a closure which permits
the extraction or introduction of liquid by means of a cannula
while avoiding entrainment of liquids upon the outside surface of
the cannula and, furthermore, insures a continuous largely
dust-free closure following introduction of the cannula.
An object of the invention is to provide an improved reaction
vessel having a closure means which automatically cleans the
external surface of the cannula that is used to pierce it.
Another object of the invention is to provide an improved reaction
vessel having a closure means which does not require removal during
extraction of samples therefrom.
Another object of the invention is to provide an improved reaction
vessel having a closure means which provides dust-free closure
following introduction of a cannula and after extraction
thereof.
Another object of the invention is to provide an improved reaction
vessel that can be heated without danger of having the contents
force complete removal of the closure means.
In accordance with the invention, there is provided a reaction
vessel having a closure comprising a pierceable foil. The thickness
of this foil is related to its elasticity such that when the foil
is pierced by a cannula, the elastic deformation of the prick hole
edge effects a bearing contact of the foil on the external wall of
the cannula.
In one illustrative embodiment of the invention, the foil is joined
to the upper edge of the vessel by welding. In another illustrative
embodiment of the invention, a hollow-type cover is employed
adapted for insertion within the throat of the vessel. In this
latter embodiment, the bottom of the cover comprises the
aforementioned foil and exhibits the characteristics hereinbefore
described.
A more complete understanding of the objects and features of the
present invention will be available following a consideration of
the specific illustrative embodiments shown in the illustrated
drawings.
BRIEF DESCRIPTION OF THE DRAWINGS
FIG. 1 shows a cross-sectional view through a vessel having a
closure according to one illustrative embodiment of the invention;
and
FIG. 2 shows a cross-sectional view through a vessel having a
closure according to a second illustrative embodiment of the
invention.
DESCRIPTION OF THE PREFERRED EMBODIMENT
With reference to FIG. 1, it will be seen that a typical reaction
vessel 1 is illustrated. The upper edge of this reaction vessel has
an outwardly extending edge flange 2. A foil 3 is stretched over
this edge flange and is welded to the periphery thereof. The foil
has a thickness with respect to its elasticity such that when it is
pierced by a sharpened cannula, the elastic deformation of the
prick hole edge effects a bearing contact upon the walls of the
cannula.
In use, it has been found that a suction cannula sharpened with an
angle smaller than 20.degree. may be advantageously employed. When
utilizing such a cannula with a foil having a thickness of about
0.1 mm., as the foil is pierced by the cannula, it arches downward
to a certain degree causing an elastic expansion at the instant of
piercing. This expansion recedes following the piercing so that due
to the elasticity of the foil, it hugs closely to the cannula wall
about the periphery of the prick hole edge. When the cannula is
later extracted, a good wiping effect is achieved. Still further,
when the cannula is extracted, there remains at most only a very
small opening and therefore the covering is substantially
preserved. It has been found that in order to achieve the desired
wiping effect, a foil thickness of 0.08 to 0.35 mm. is advantageous
and a foil thickness ranging from 0.1 to 0.3 mm. is preferable.
The foil 3 in FIG. 1 is preferably affixed to the vessel edges 1
under slight tension. With this type of construction, a thickness
of foil in the lower range of the above-cited range will be
preferred. Advantageously, a foil thickness of about 0.1 mm. can be
used for this welded closure arrangement. It has been discovered
that with the welded foil closure, it is possible to heat the
reaction vessel over 100.degree. C. Under the resulting pressure,
an unpierced foil closure will expand and arch due to the
volumetric expansion of the contents of the vessel; nevertheless,
when subsequently pierced, the area of the foil surrounding the
prick will retain enough natural elasticity to effect the desired
wiping of the cannula when it is extracted.
An ancillary feature of the present invention resides in the fact
that the prick hole also fulfills an identification function. As a
result of the prick hole, there is a discrete mark on the vessel
cover which indicates with certainty that the particular vessel has
been served with the cannula. In automatic processing systems
particularly, this positive indication of prior processing may be
of extreme importance.
FIG. 2 illustrates another top closure utilizing the principles of
the present invention. As shown in this FIG., a hollow cover 4 is
inserted into the upper edge of the vessel 1. The dimensions of the
hollow cover are such that it is pressed into the upwardly
chamfered throat of the reaction vessel. The bottom of cover 4 is
formed of a foil 5 which may be present, for example, at the lower
section of the hollow cover edge 6. As a result of the hollow cover
edge, it is possible upon insertion to achieve a satisfactory
edgewise support so that a somewhat greater foil thickness is
appropriate. This greater thickness permits certain advantages and
heightens the desirable effects achievable through the elasticity
of the cover. In fact, it has been found advantageous with closures
of the type shown in FIG. 2 to utilize a foil thickness in the
range between 0.2 and 0.3 mm. Within this size range, one finds
that the cover tends to fit tightly to the vessel and there is
little danger of loosening during cannula extraction or as a result
of increased pressure within the vessel itself.
In connection with the closure shown in FIG. 2, the somewhat
thicker dimension of the foil proves advantageous because during
extraction of a cannula, there is a canting or upward tilting of
the prick hole edge which provides a greater wiping surface and
consequently a better wiping effect.
The preceding description has set forth two specific embodiments of
the invention. No detailed comments concerning the material of
either the reaction vessel or the closure means have been made.
Advantageously, the foil, like the vessel, may be made of a
polypropylene-based plastic. On the other hand, there is no
intention to limit the invention to the use of any particular
material. It is intended in the following claims to include all
such modifications and variations of the disclosed embodiments as
come within the skill of those in the art.
* * * * *