U.S. patent application number 17/825346 was filed with the patent office on 2022-09-29 for method for removing and/or detecting nucleic acids having mismatched nucleotides. This patent application is currently assigned to New England Biolabs, Inc.. The applicant listed for this patent is New England Biolabs, Inc.. Invention is credited to Andrew F. Gardner.
Application Number | 20220307081 17/825346 |
Document ID | / |
Family ID | 1000006379735 |
Filed Date | 2022-09-29 |
United States Patent Application | 20220307081 |
Kind Code | A1 |
Gardner; Andrew F. | September 29, 2022 |
Provided herein, among other things, are various in vitro methods that involve cleaving dsDNA molecules that comprise a mismatched nucleotide using EndoMS. In some embodiments, the method may comprise ligating a T-tailed double-stranded adapter to A-tailed double-stranded fragments of nucleic acid to produce ligation products that comprise adapter-ligated fragments and double-stranded adapter dimers that comprise a T:T mismatch at the ligation junction and cleaving both strands of the adapter dimers using EndoMS.
Inventors: | Gardner; Andrew F.; (Manchester, MA) | ||||||||||
Applicant: |
|
||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
Assignee: | New England Biolabs, Inc. Ipswich MA |
||||||||||
Family ID: | 1000006379735 | ||||||||||
Appl. No.: | 17/825346 | ||||||||||
Filed: | May 26, 2022 |
Application Number | Filing Date | Patent Number | ||
---|---|---|---|---|
16616631 | Nov 25, 2019 | 11371088 | ||
PCT/US18/36875 | Jun 11, 2018 | |||
17825346 | ||||
62525803 | Jun 28, 2017 | |||
Current U.S. Class: | 1/1 |
Current CPC Class: | C12Q 1/6874 20130101; C12N 9/22 20130101; C12N 9/93 20130101; C12Q 1/6855 20130101 |
International Class: | C12Q 1/6855 20060101 C12Q001/6855; C12N 9/22 20060101 C12N009/22; C12N 9/00 20060101 C12N009/00; C12Q 1/6874 20060101 C12Q001/6874 |
Sequence CWU 1
1
1091260PRTMethanobrevibacter 1Met Lys Tyr Lys Ile Leu Glu Asn Pro
Asn Cys Glu Asp Ala Tyr Gly1 5 10 15Leu Ile Glu Glu Ala Leu Arg Lys
Arg Ala Thr Ile Thr Ile Tyr Ala 20 25 30Cys Cys Lys Val Asn Tyr Glu
Gly Arg Ala Leu Ser Glu Leu Asn Trp 35 40 45Gly Glu Arg Ile Ile Leu
Ile Lys Pro Asp Gly Ser Phe Leu Ile His 50 55 60Gln Glu Lys Lys Val
Glu Pro Val Asn Trp Gln Pro Pro Lys Ser Lys65 70 75 80Thr Arg Gly
Tyr Ile Gln Asp Asn Asn Leu Ile Leu Glu Ser His Arg 85 90 95Arg Thr
Pro Lys Glu Leu Leu Thr Val Glu Ile Arg Lys Val Gln Tyr 100 105
110Ile Thr Tyr Ala Asn Ile Glu Asp Phe Glu Glu Leu Glu Gln Ala Gly
115 120 125Tyr Glu Lys Asp Met Gly Asp Met Ile Met Glu Lys Pro His
Met Ile 130 135 140Glu Glu Gly Phe Lys Pro Thr Ala Arg Glu Tyr Ser
Val Glu His Gly145 150 155 160Phe Ile Asp Ile Leu Gly Lys Asp Cys
Asp Asn Asn Leu Met Ile Leu 165 170 175Glu Leu Lys Ala Arg Lys Ala
Gly Val Ser Ala Val Lys Gln Leu Lys 180 185 190Arg Tyr Leu Thr Asp
Phe Glu Asp Asp Asp Asn Asp Tyr Leu Lys Glu 195 200 205Cys Leu Val
Gln Lys Lys Lys Ile Arg Gly Leu Leu Val Ala Pro Ser 210 215 220Leu
Gly Glu Asp Ala Lys Glu Leu Ile Glu Lys Glu Gly Ile Glu Phe225 230
235 240Val Ala Val Asn Pro Pro Lys Glu Leu Lys Arg Asp Lys Lys Val
Thr 245 250 255Leu Asp Ala Phe 2602260PRTMethanobrevibacter smithii
2Met Lys Tyr Lys Ile Leu Glu Asn Pro Asn Cys Glu Asp Ala Tyr Gly1 5
10 15Leu Ile Glu Glu Ala Leu Arg Lys Arg Ala Thr Ile Thr Ile Tyr
Ala 20 25 30Cys Cys Lys Val Asn Tyr Glu Gly Arg Ala Leu Ser Glu Leu
Asn Trp 35 40 45Gly Glu Arg Ile Ile Leu Ile Lys Pro Asp Gly Ser Phe
Leu Ile His 50 55 60Gln Glu Lys Lys Val Glu Pro Val Asn Trp Gln Pro
Pro Lys Ser Lys65 70 75 80Thr Arg Gly Tyr Ile Gln Asp Asn Asn Leu
Ile Leu Glu Ser His Arg 85 90 95Arg Thr Pro Lys Glu Leu Leu Thr Val
Glu Ile Arg Lys Val Gln Tyr 100 105 110Ile Thr Tyr Ala Asn Ile Glu
Asp Phe Glu Glu Leu Glu Gln Ala Gly 115 120 125Tyr Glu Lys Asp Met
Gly Asp Met Ile Met Glu Lys Pro His Met Ile 130 135 140Glu Glu Gly
Phe Lys Pro Thr Ala Arg Glu Tyr Ser Val Glu His Gly145 150 155
160Phe Ile Asp Ile Leu Gly Lys Asp Cys Asp Asn Asn Leu Met Ile Leu
165 170 175Glu Leu Lys Ala Arg Lys Ala Gly Val Ser Ala Val Lys Gln
Leu Lys 180 185 190Arg Tyr Leu Thr Asp Phe Glu Asp Gly Asp Asn Asp
Tyr Leu Lys Glu 195 200 205Cys Leu Val Gln Lys Lys Lys Ile Arg Gly
Leu Leu Val Ala Pro Ser 210 215 220Leu Gly Glu Asp Ala Lys Glu Leu
Ile Glu Lys Glu Gly Ile Glu Phe225 230 235 240Val Ala Val Asn Pro
Pro Lys Glu Leu Lys Arg Asp Lys Lys Val Thr 245 250 255Leu Asp Ala
Phe 2603260PRTMethanobrevibacter smithii 3Met Lys Tyr Lys Ile Leu
Glu Asn Pro Asn Cys Glu Asp Ala Tyr Gly1 5 10 15Leu Val Glu Glu Ala
Leu Arg Lys Lys Ala Thr Ile Thr Ile Tyr Ala 20 25 30Cys Cys Lys Val
Thr Tyr Glu Gly Arg Ala Leu Ser Glu Leu Asn Trp 35 40 45Gly Glu Arg
Ile Ile Leu Ile Lys Pro Asp Gly Ser Phe Leu Ile His 50 55 60Gln Glu
Lys Lys Val Glu Pro Val Asn Trp Gln Pro Pro Lys Ser Lys65 70 75
80Thr Arg Gly Tyr Ile Gln Asp Asp Asn Leu Ile Leu Glu Ser His Arg
85 90 95Arg Thr Pro Lys Glu Leu Leu Ile Val Glu Ile Arg Lys Val Gln
Tyr 100 105 110Ile Thr Tyr Ala Asn Ile Glu Asp Phe Glu Glu Leu Glu
Gln Ala Gly 115 120 125Tyr Glu Lys Asp Met Gly Asp Met Ile Met Glu
Lys Pro His Met Ile 130 135 140Glu Glu Gly Phe Lys Pro Thr Ala Arg
Glu Tyr Ser Val Glu His Gly145 150 155 160Phe Ile Asp Ile Leu Gly
Lys Asp Arg Asp Asn Asn Leu Met Ile Leu 165 170 175Glu Leu Lys Ala
Arg Lys Ala Gly Val Ser Ala Val Lys Gln Leu Lys 180 185 190Arg Tyr
Leu Thr Asp Phe Glu Asp Asp Asp Asn Asp Tyr Leu Lys Glu 195 200
205Cys Arg Val Gln Lys Lys Lys Ile Arg Gly Leu Leu Val Ala Pro Ser
210 215 220Leu Gly Asn Asp Ala Glu Glu Leu Leu Glu Lys Glu Gly Ile
Glu Phe225 230 235 240Val Ala Val Glu Pro Pro Lys Glu Leu Lys Arg
Asp Lys Lys Val Thr 245 250 255Leu Asp Ala Phe
2604260PRTMethanobrevibacter smithii 4Met Lys Tyr Lys Ile Leu Glu
Asn Pro Asn Cys Glu Asp Ala Tyr Gly1 5 10 15Leu Val Glu Glu Ala Leu
Arg Lys Lys Ala Thr Ile Thr Ile Tyr Ala 20 25 30Cys Cys Lys Val Thr
Tyr Glu Gly Arg Ala Leu Ser Glu Leu Asn Trp 35 40 45Gly Glu Arg Ile
Ile Leu Ile Lys Pro Asp Gly Ser Phe Leu Ile His 50 55 60Gln Glu Lys
Lys Val Glu Pro Val Asn Trp Gln Pro Pro Lys Ser Lys65 70 75 80Thr
Arg Gly Tyr Ile Gln Asp Asp Asn Leu Ile Leu Glu Ser His Arg 85 90
95Arg Thr Pro Lys Glu Leu Leu Ile Val Glu Ile Arg Lys Val Gln Tyr
100 105 110Ile Thr Tyr Ala Asn Ile Glu Asp Phe Glu Glu Leu Glu Gln
Ala Gly 115 120 125Tyr Glu Lys Asp Met Gly Asp Met Ile Met Lys Lys
Pro His Met Ile 130 135 140Glu Glu Gly Phe Lys Pro Thr Ala Arg Glu
Tyr Ser Val Glu His Gly145 150 155 160Phe Ile Asp Ile Leu Gly Lys
Asp Arg Asp Asn Asn Leu Met Ile Leu 165 170 175Glu Leu Lys Ala Arg
Lys Ala Gly Val Ser Ala Val Lys Gln Leu Lys 180 185 190Arg Tyr Leu
Thr Asp Phe Glu Asp Asp Asp Asn Asp Tyr Leu Lys Glu 195 200 205Cys
Arg Val Gln Lys Lys Lys Ile Arg Gly Leu Leu Val Ala Pro Ser 210 215
220Leu Gly Asn Asp Ala Glu Glu Leu Leu Glu Lys Glu Gly Ile Glu
Phe225 230 235 240Val Ala Val Glu Pro Pro Lys Glu Leu Lys Arg Asp
Lys Lys Val Thr 245 250 255Leu Asp Ala Phe
2605260PRTMethanobrevibacter smithii 5Met Lys Tyr Lys Ile Leu Glu
Asn Pro Asn Cys Glu Asp Ala Tyr Gly1 5 10 15Leu Val Glu Glu Ala Leu
Arg Lys Lys Ala Thr Ile Thr Ile Tyr Ala 20 25 30Cys Cys Lys Val Thr
Tyr Glu Gly Arg Ala Leu Ser Glu Leu Asn Trp 35 40 45Gly Glu Arg Ile
Ile Leu Ile Lys Pro Asp Gly Ser Phe Leu Ile His 50 55 60Gln Glu Lys
Lys Val Glu Pro Val Asn Trp Gln Pro Pro Lys Ser Lys65 70 75 80Thr
Arg Gly Tyr Ile Gln Asp Asp Asn Leu Ile Leu Glu Ser His Arg 85 90
95Arg Thr Pro Lys Glu Leu Leu Ile Val Glu Ile Arg Lys Val Gln Tyr
100 105 110Ile Thr Tyr Ala Asn Ile Glu Asp Phe Glu Glu Leu Glu Gln
Ala Gly 115 120 125Tyr Glu Lys Asp Met Gly Asp Met Ile Met Glu Lys
Pro His Met Ile 130 135 140Glu Glu Gly Phe Lys Pro Thr Ala Arg Glu
Tyr Ser Val Glu His Gly145 150 155 160Phe Ile Asp Ile Leu Gly Lys
Asp Arg Asn Asn Asn Leu Met Ile Leu 165 170 175Glu Leu Lys Ala Arg
Lys Ala Gly Val Ser Ala Val Lys Gln Leu Lys 180 185 190Arg Tyr Leu
Thr Asp Phe Glu Asp Asp Asp Asn Asp Tyr Leu Lys Glu 195 200 205Cys
Arg Val Gln Lys Lys Lys Ile Arg Gly Leu Leu Val Ala Pro Ser 210 215
220Leu Gly Asn Asp Ala Glu Glu Leu Leu Glu Lys Glu Gly Ile Glu
Phe225 230 235 240Val Ala Val Glu Pro Pro Lys Glu Leu Lys Arg Asp
Lys Lys Val Thr 245 250 255Leu Asp Ala Phe
2606260PRTMethanobrevibacter oralis 6Met Lys Tyr Arg Ile Leu Glu
Arg Pro Ser Cys Glu Glu Gly Tyr Asp1 5 10 15Leu Val Glu Glu Ala Leu
Arg Lys Lys Ala Thr Ile Ile Ile Phe Ala 20 25 30Cys Cys Lys Val Asn
Tyr Glu Gly Arg Ala Leu Ser Gln Leu Asn Trp 35 40 45Gly Glu Arg Ile
Ile Leu Ile Lys Pro Asp Gly Ser Phe Leu Ile His 50 55 60Gln Glu Arg
Lys Val Asp Pro Val Asn Trp Gln Pro Pro Lys Ser Arg65 70 75 80Thr
Arg Ser Phe Ile Arg Asn Asp Lys Leu Ile Leu Glu Ser His Arg 85 90
95Arg Val Pro Lys Glu Leu Leu Ser Val Glu Ile Arg Lys Ile Gln Phe
100 105 110Ile Asn Tyr Ala Asn Val Glu Asp Phe Glu Glu Leu Glu Gln
Ala Gly 115 120 125Tyr Glu Lys Asp Met Gly Asp Met Ile Met Asp Lys
Pro His Leu Ile 130 135 140Glu Glu Gly Phe Thr Pro Thr Ala Arg Glu
Tyr Ser Val Glu His Gly145 150 155 160Phe Ile Asp Ile Leu Gly Lys
Asp Asp Asp Asn Asn Leu Met Val Leu 165 170 175Glu Leu Lys Ala Arg
Lys Ala Gly Val Ser Ala Val Lys Gln Leu Lys 180 185 190Arg Tyr Leu
Ser Asp Phe Glu Asn Thr Glu Asn Asp Tyr Leu Lys Glu 195 200 205Cys
Lys Ala Ser Lys Lys Arg Ile Arg Gly Leu Leu Val Ala Pro Ser 210 215
220Leu Gly Glu Asp Ala Lys Glu Met Ile Glu Asp Glu Gly Ile Glu
Phe225 230 235 240Val Ser Ile Glu Pro Pro Lys Glu Leu Arg Arg Asp
Lys Lys Thr Thr 245 250 255Leu Asp Ala Phe
2607260PRTMethanobrevibacter gottschalkii 7Met Lys Tyr Lys Ile Leu
Glu Lys Pro Ser Cys Glu Asp Gly Tyr Asp1 5 10 15Leu Val Gln Glu Ala
Leu Arg Lys Lys Ala Thr Ile Leu Ile Phe Ala 20 25 30Cys Cys Lys Val
Ser Tyr Glu Gly Arg Ala Leu Ser Glu Leu Asn Trp 35 40 45Gly Glu Arg
Ile Ile Met Ile Lys Pro Asp Gly Thr Phe Leu Ile His 50 55 60Gln Glu
Lys Lys Val Glu Pro Val Asn Trp Gln Pro Pro Lys Ser Arg65 70 75
80Thr Arg Ser Tyr Ile Lys Asn Asp Asn Leu Phe Leu Glu Ser His Arg
85 90 95Arg Thr Pro Lys Glu Leu Leu Thr Val Glu Ile Arg Lys Ile Gln
Phe 100 105 110Ile Asn Tyr Ala Asn Ile Glu Asp Phe Glu Glu Leu Glu
Gln Ala Gly 115 120 125Tyr Glu Lys Asp Met Gly Asp Met Ile Met Glu
Lys Pro His Met Ile 130 135 140Glu Glu Gly Phe Thr Pro Thr Ala Arg
Glu Tyr Ser Val Glu His Gly145 150 155 160Phe Ile Asp Ile Leu Gly
Lys Asp Arg Asp Asn Asn Leu Met Val Leu 165 170 175Glu Leu Lys Ala
Arg Lys Ala Gly Ile Thr Ala Val Lys Gln Leu Arg 180 185 190Arg Tyr
Leu Gln Asp Leu Glu Asn Thr Asp Asn Asp Tyr Leu Lys Glu 195 200
205Cys Glu Ser Gln Lys Lys Lys Ile Arg Gly Leu Leu Val Ala Pro Ser
210 215 220Ile Met Asp Asp Ala Leu Glu Leu Leu Glu Asn Glu Gly Ile
Glu Phe225 230 235 240Val Ser Ile Glu Pro Pro Arg Glu Leu Lys Arg
Asp Lys Lys Val Thr 245 250 255Leu Asp Ala Phe
2608260PRTMethanobrevibacter 8Met Lys Tyr Lys Ile Leu Glu Lys Pro
Ser Cys Glu Asp Gly Tyr Asp1 5 10 15Leu Val Gln Glu Ala Leu Arg Lys
Lys Thr Thr Ile Leu Ile Phe Ala 20 25 30Cys Cys Lys Val Ser Tyr Glu
Gly Arg Ala Leu Ser Glu Leu Asn Trp 35 40 45Gly Glu Arg Ile Ile Met
Ile Lys Pro Asp Gly Thr Phe Leu Ile His 50 55 60Gln Glu Lys Lys Val
Glu Pro Val Asn Trp Gln Pro Pro Lys Ser Arg65 70 75 80Thr Arg Ser
Tyr Ile Lys Asn Asp Asn Leu Phe Leu Glu Ser His Arg 85 90 95Arg Thr
Pro Lys Glu Leu Leu Thr Val Glu Ile Arg Lys Ile Gln Phe 100 105
110Ile Asn Tyr Ala Asn Ile Glu Asp Phe Glu Glu Leu Glu Gln Ala Gly
115 120 125Tyr Glu Lys Asp Met Gly Asp Met Ile Met Glu Lys Pro His
Met Ile 130 135 140Glu Glu Gly Phe Thr Pro Thr Ala Arg Glu Tyr Ser
Val Glu His Gly145 150 155 160Phe Ile Asp Ile Leu Gly Lys Asp Ser
Asp Asn Asn Leu Met Val Leu 165 170 175Glu Leu Lys Ala Arg Lys Ala
Gly Ile Thr Ala Val Lys Gln Leu Arg 180 185 190Arg Tyr Leu Gln Asp
Leu Glu Asn Thr Asp Asn Asp Tyr Leu Lys Glu 195 200 205Cys Glu Ser
Gln Lys Lys Lys Ile Arg Gly Leu Leu Val Ala Pro Ser 210 215 220Ile
Met Asp Asp Ala Leu Glu Leu Leu Glu Asn Glu Gly Ile Glu Phe225 230
235 240Val Ser Ile Glu Pro Pro Arg Glu Leu Lys Arg Asp Lys Lys Val
Thr 245 250 255Leu Asp Ala Phe 2609260PRTMethanobrevibacter
millerae 9Met Lys Tyr Lys Ile Leu Glu Lys Pro Asp Cys Glu Lys Ala
Tyr Glu1 5 10 15Leu Val Glu Glu Ala Met Arg Lys Arg Ala Thr Ile Thr
Leu Phe Ala 20 25 30Cys Cys Lys Val Glu Tyr Glu Gly Arg Ala Leu Ser
Gln Leu Asn Trp 35 40 45Gly Glu Arg Ile Ile Leu Ile Lys Pro Asp Gly
Ser Phe Leu Ile His 50 55 60Gln Asp Lys Lys Val Glu Pro Val Asn Trp
Gln Pro Pro Lys Ser Lys65 70 75 80Thr Arg Ser Tyr Leu Ser Gly Glu
Arg Leu Ile Leu Glu Ser His Arg 85 90 95Arg Thr Pro Lys Glu Leu Leu
Thr Val Glu Val Arg Gln Ile Gln Phe 100 105 110Ile Ser Tyr Ala Asn
Met Glu Asp Phe Glu Glu Leu Glu Gln Ala Gly 115 120 125Tyr Glu Lys
Asp Met Ser Asp Met Ile Met Glu Arg Pro His Leu Ile 130 135 140Glu
Glu Gly Phe Thr Pro Lys Thr Arg Glu Tyr Ser Val Glu His Gly145 150
155 160Phe Ile Asp Ile Leu Gly Lys Asp Asn Asp Gly Asn Leu Met Val
Leu 165 170 175Glu Leu Lys Ala Arg Lys Ala Gly Val Ser Ala Val Lys
Gln Ile Arg 180 185 190Arg Tyr Leu Gln Asp Leu Glu Asn Thr Glu Asn
Asp Tyr Leu Lys Glu 195 200 205Cys Lys Ala Gln Lys Lys Lys Ile Arg
Gly Ile Leu Val Ala Pro Ser 210 215 220Ile Met Glu Asp Ala Arg Glu
Met Ile Glu Glu Glu Gly Ile Glu Phe225 230 235 240Val Ser Val Glu
Pro Pro Arg Glu Leu Lys Arg Asp Lys Lys Val Thr 245 250 255Leu Asp
Ala Phe 26010260PRTMethanobrevibacter 10Met Lys Tyr Lys Ile Leu Glu
Lys Pro Asn Cys Glu Asp Ala Tyr Glu1 5 10 15Leu Val Gln Asp Ala Leu
Arg Lys Lys Ala Thr Ile Ile Ile Phe Ala 20 25 30Cys Cys Lys Val Asn
Tyr Glu Gly Arg Ala Leu Ser Glu Leu Asn Trp 35 40 45Gly Glu Arg Ile
Ile Met Ile Lys Pro Asp Gly Ala Phe Leu Ile His 50 55 60Gln Glu Lys
Lys Val Glu Pro Val Asn Trp Gln Pro Pro Lys Ser Arg65 70
75 80Thr Arg Ser Tyr Ile Lys Asn Glu Asn Leu Phe Leu Glu Ser His
Arg 85 90 95Arg Thr Pro Lys Glu Leu Leu Thr Ala Glu Ile Arg Gln Ile
Gln Phe 100 105 110Ile Ser Tyr Ala Asn Ile Glu Asp Phe Glu Glu Leu
Glu Gln Ala Gly 115 120 125Tyr Glu Lys Asp Met Ser Asp Met Ile Met
Glu Lys Pro His Leu Ile 130 135 140Glu Glu Gly Phe Thr Pro Thr Thr
Arg Glu Tyr Ser Val Glu His Gly145 150 155 160Phe Ile Asp Ile Leu
Gly Lys Asp Ser Asp Asn Asn Leu Met Val Leu 165 170 175Glu Leu Lys
Ala Arg Lys Ala Gly Val Ala Ala Val Lys Gln Leu Arg 180 185 190Arg
Tyr Leu Gln Asp Leu Glu Asn Thr Asp Asn Asp Tyr Leu Lys Glu 195 200
205Cys Lys Ala Gln Lys Lys Lys Ile Arg Gly Leu Leu Val Ala Pro Ser
210 215 220Ile Met Asp Asp Ala Leu Glu Leu Ile Glu Glu Glu Gly Ile
Glu Phe225 230 235 240Val Ser Ile Glu Pro Pro Arg Glu Leu Lys Arg
Asp Lys Lys Val Thr 245 250 255Leu Asp Ala Phe
26011254PRTMethanobrevibacter oralis 11Met Lys Tyr Arg Ile Leu Glu
Arg Pro Ser Cys Glu Glu Gly Tyr Asp1 5 10 15Leu Val Glu Glu Ala Leu
Arg Lys Lys Ala Thr Ile Ile Ile Phe Ala 20 25 30Cys Cys Lys Val Asn
Tyr Glu Gly Arg Ala Leu Ser Gln Leu Asn Trp 35 40 45Gly Glu Arg Ile
Ile Leu Ile Lys Pro Asp Gly Ser Phe Leu Ile His 50 55 60Gln Glu Arg
Lys Val Asp Pro Val Asn Trp Gln Pro Pro Lys Ser Arg65 70 75 80Thr
Arg Ser Phe Ile Arg Asn Asp Lys Leu Ile Leu Glu Ser His Arg 85 90
95Arg Val Pro Lys Glu Leu Leu Ser Val Glu Ile Arg Lys Ile Gln Phe
100 105 110Ile Asn Tyr Ala Asn Val Glu Asp Phe Glu Glu Leu Glu Gln
Ala Gly 115 120 125Tyr Glu Lys Asp Met Gly Asp Met Ile Met Asp Lys
Pro His Leu Ile 130 135 140Glu Glu Gly Phe Thr Pro Thr Ala Arg Glu
Tyr Ser Val Glu His Gly145 150 155 160Phe Ile Asp Ile Leu Gly Lys
Asp Asp Asp Asn Asn Leu Met Val Leu 165 170 175Glu Leu Lys Ala Arg
Lys Ala Gly Val Ser Ala Val Lys Gln Leu Lys 180 185 190Arg Tyr Leu
Ser Asp Phe Glu Asn Thr Glu Asn Asp Tyr Leu Lys Glu 195 200 205Cys
Lys Ala Ser Lys Lys Arg Ile Arg Gly Leu Leu Val Ala Pro Ser 210 215
220Leu Gly Glu Asp Ala Lys Glu Met Ile Glu Asp Glu Gly Ile Glu
Phe225 230 235 240Val Ser Ile Glu Pro Pro Lys Glu Leu Arg Arg Asp
Lys Lys 245 25012260PRTMethanobrevibacter cuticularis 12Met Lys Tyr
Lys Leu Leu Glu Asn Pro Asn Ile Glu Glu Met Tyr Asp1 5 10 15Leu Ile
Glu Glu Gly Leu Arg Lys Lys Ala Met Ile Asn Val Phe Cys 20 25 30Cys
Cys Lys Val Ile Tyr Glu Gly Arg Ala Leu Ser Gln Leu Asp Phe 35 40
45Gly Glu Arg Met Ile Leu Leu Lys Pro Asp Gly Ser Phe Leu Ile His
50 55 60Gln Glu Arg Lys Val Asp Pro Val Asn Trp Gln Pro Pro Lys Ser
Arg65 70 75 80Thr Arg Thr Phe Ile Lys Asp Asn Thr Leu Phe Leu Glu
Ser His Arg 85 90 95Arg Ser Pro Lys Glu Arg Leu Glu Val Glu Ile Lys
Lys Thr His Phe 100 105 110Val Asn Tyr Val Leu Val Glu Asp Tyr Gln
Glu Leu Glu Ile Ala Gly 115 120 125Tyr Glu Lys Asp Met Gly Asp Met
Ile Met Lys His Pro His Met Ile 130 135 140Glu Glu Gly Phe Lys Pro
Thr Asp Arg Glu Tyr Ser Thr Glu His Gly145 150 155 160Phe Ile Asp
Ile Leu Gly Lys Asp Lys Glu Gly Asn Leu Met Val Leu 165 170 175Glu
Leu Lys Cys Arg Lys Ala Gly Ile Asn Ala Val Lys Gln Ile Arg 180 185
190Arg Tyr Leu Thr Asp Phe Lys Glu Glu Glu Asn Ser Asn Leu Glu Asn
195 200 205Thr Gly Asn Glu Lys Lys Lys Val Arg Gly Leu Leu Val Ala
Pro Ser 210 215 220Ile Gly Glu Asp Ala Arg Glu Leu Leu Glu Glu Glu
Asn Ile Glu Phe225 230 235 240Lys Ser Ile Asp Pro Pro Lys Glu Leu
Lys Asn Asp Lys Lys Val Thr 245 250 255Leu Asp Ile Phe
26013260PRTMethanobrevibacter curvatus 13Met Lys Phe Lys Gly Lys
Glu Asp Pro Asn Ile Asp Glu Ser Phe Asp1 5 10 15Leu Ile Asp Glu Gly
Leu Arg Lys Lys Ala Thr Ile Val Ile Phe Ala 20 25 30Cys Cys Lys Val
Ile Tyr Glu Gly Arg Ala Ile Ser Gln Leu Asp Phe 35 40 45Gly Glu Arg
Ile Ile Leu Ile Lys Pro Asp Gly Ser Phe Leu Ile His 50 55 60Gln Asp
Lys Lys Val Asp Pro Val Asn Trp Gln Pro Pro Lys Ser Arg65 70 75
80Ser Arg Val Leu Ile Arg Asn Glu Lys Leu Phe Leu Glu Ser Phe Arg
85 90 95Arg Thr Pro Arg Glu His Leu Glu Val Glu Ile Arg Lys Ile His
Phe 100 105 110Leu Asn Tyr Ala Leu Ile Glu Asp Tyr Gln Glu Leu Glu
Ile Ala Gly 115 120 125Tyr Glu Lys Asp Leu Gly Asp Leu Ile Ile Asp
Lys Pro His Leu Ile 130 135 140Glu Glu Gly Phe Lys Pro Thr Asn Arg
Glu Tyr Ser Thr Glu His Gly145 150 155 160Phe Ile Asp Ile Leu Gly
Asn Asp Lys Glu Gly Asn Leu Met Val Leu 165 170 175Glu Leu Lys Ser
Arg Lys Ile Gly Val Asn Ala Val Lys Gln Ile Arg 180 185 190Arg Tyr
Leu Ser Asp Phe Glu Asp Glu Lys Asn Ser Tyr Leu Lys Asp 195 200
205Leu Gly Val Glu Lys Lys Lys Ile Arg Gly Leu Leu Val Ala Pro Lys
210 215 220Ile Asp Glu Asp Ala Lys Glu Met Ile Glu Glu Glu Gly Ile
Glu Phe225 230 235 240Val Ser Leu Glu Pro Pro Lys Glu Leu Lys Arg
Asp Lys Leu Val Thr 245 250 255Leu Asp Ser Phe
26014260PRTMethanobrevibacter olleyae 14Met Lys Tyr Lys Ile Leu Glu
Asn Pro Asn Tyr Glu Glu Thr Tyr Asp1 5 10 15Leu Ile Glu Glu Gly Leu
Lys Lys Lys Ala Thr Ile Leu Leu Phe Ala 20 25 30Cys Cys Arg Val Ser
Tyr Glu Gly Arg Ser Leu Ser Glu Leu Asp Tyr 35 40 45Gly Glu Arg Ile
Ile Met Met Lys Pro Asp Gly Cys Phe Leu Ile His 50 55 60Gln Asp Asn
Lys Val Asp Pro Val Asn Trp Gln Pro Pro Lys Ser Arg65 70 75 80Pro
Arg Ala Tyr Ile Lys Asp Glu Ile Leu Phe Leu Glu Ser His Arg 85 90
95Arg Ser Pro Pro Glu Arg Ile Glu Val Glu Ile Lys Lys Val His Tyr
100 105 110Ala Asn Tyr Asn Leu Ile Glu Asp Tyr Glu Glu Leu Glu Arg
Ala Gly 115 120 125Tyr Glu Lys Asp Met Gly Asp Met Ile Trp Asp Lys
Pro His Ile Ile 130 135 140Glu Glu Gly Phe Arg Pro Thr Val Arg Glu
Tyr Ala Thr Glu His Gly145 150 155 160Phe Ile Asp Ile Leu Gly Lys
Asp Ser Lys Gly Asn Leu Met Val Leu 165 170 175Glu Leu Lys Ser Arg
Lys Ala Gly Ile Thr Ala Val Lys Gln Ile Arg 180 185 190Arg Tyr Leu
Thr Asp Phe Glu Asn Lys Glu Asn Ser Glu Ile Lys Ala 195 200 205Lys
Asn Gly Gln Lys Gln Lys Ile Arg Gly Leu Leu Val Ala Pro Ser 210 215
220Ile Gly Asp Asp Ala Leu Glu Leu Leu Glu Glu Glu Gly Ile Glu
Phe225 230 235 240Val Ser Val Glu Pro Pro Arg Glu Leu Lys Lys Asp
Lys Arg Val Thr 245 250 255Leu Asp Ser Phe
26015259PRTMethanobrevibacter ruminantium 15Met Lys Tyr Lys Ile Leu
Glu Asn Pro Asn Tyr Lys Glu Ala Tyr Glu1 5 10 15Leu Ile Glu Glu Gly
Leu Val Lys Lys Ala Thr Met Leu Leu Phe Ala 20 25 30Cys Cys Lys Val
Ser Tyr Glu Gly Arg Ala Leu Ser Glu Leu Asp Tyr 35 40 45Gly Glu Arg
Ile Ile Met Ile Lys Pro Asp Gly Cys Phe Leu Ile His 50 55 60Gln Asp
Asn Lys Val Asp Pro Val Asn Trp Gln Pro Pro Lys Ser Lys65 70 75
80Thr Lys Ala Leu Ile Lys Asp Glu Thr Leu Tyr Leu Glu Ser His Arg
85 90 95Arg Lys Pro Pro Glu Leu Leu Glu Val Glu Val Lys Lys Ile His
Tyr 100 105 110Ala Arg Tyr Asn Leu Ile Glu Asp Tyr Glu Glu Leu Glu
Arg Ala Gly 115 120 125Tyr Glu Lys Asp Met Gly Asp Met Ile Trp Asp
Asn Pro His Ile Ile 130 135 140Glu Glu Gly Phe Arg Pro Thr Val Arg
Glu Tyr Ala Thr Glu His Gly145 150 155 160Phe Ile Asp Ile Leu Gly
Lys Asp Ala Asp Gly Asn Leu Met Val Leu 165 170 175Glu Leu Lys Ala
Arg Lys Ala Gly Ile Thr Ala Val Lys Gln Ile Arg 180 185 190Arg Tyr
Leu Thr Asp Phe Glu Asn Arg Glu Asn Lys Glu Ile Arg His 195 200
205Glu Glu Glu Lys Gln Lys Val Arg Gly Leu Leu Val Ala Pro Ser Ile
210 215 220Gly Asp Asp Ala Leu Glu Leu Leu Glu Glu Glu Gly Ile Glu
Phe Val225 230 235 240Ser Val Glu Pro Pro Arg Glu Leu Lys Lys Asp
Lys Lys Val Thr Leu 245 250 255Asp Ala
Phe16259PRTMethanobrevibacter 16Met Asn Tyr Lys Thr Ile Glu Glu Pro
Asn Thr Glu Glu Thr Tyr Asp1 5 10 15Leu Ile Glu Ala Gly Leu Arg Lys
Lys Ala Met Ile Thr Leu Phe Thr 20 25 30Tyr Cys Lys Val Glu Tyr Glu
Gly Arg Ala Leu Ser Gln Leu Gly Tyr 35 40 45Gly Glu Arg Met Ile Leu
Ile Lys Pro Asp Gly Ser Phe Leu Val His 50 55 60Gln Asp Arg Lys Val
Glu Pro Val Asn Trp Gln Pro Pro Lys Ser Lys65 70 75 80Thr Arg Val
Phe Ile Arg Asp Gly Lys Val Ile Ile Glu Ser Asn Arg 85 90 95Arg Thr
Pro Lys Glu Arg Leu Glu Val Leu Ile Glu Lys Thr Phe Ile 100 105
110Gly Thr Tyr Ala Val Val Glu Asp Tyr Glu Glu Leu Glu Gln Ala Gly
115 120 125Tyr Glu Lys Asp Met Gly Asp Met Ile Met Glu Asn Pro His
Ile Ile 130 135 140Glu Glu Gly Phe Lys Pro Thr Asp Arg Glu Tyr Asn
Val Glu His Gly145 150 155 160Phe Ile Asp Ile Leu Gly Lys Asp Ser
Asp Gly Asn Leu Met Ile Leu 165 170 175Glu Leu Lys Ser Arg Lys Ile
Gly Ile Ser Ala Val Lys Gln Ile Lys 180 185 190Arg Tyr Ile Asp Asp
Leu Thr Asn Thr Glu Asn Arg Ser Leu Arg Leu 195 200 205Gly Val Glu
Lys Lys Lys Ile Arg Gly Leu Leu Val Gly Pro Lys Ile 210 215 220Asp
Glu Asp Ala Lys Glu Met Ile Glu Glu Glu Gly Ile Glu Phe Val225 230
235 240Glu Cys Glu Pro Pro Lys Glu Leu Lys Arg Asp Lys Lys Thr Thr
Leu 245 250 255Asp Ser Phe17260PRTMethanobrevibacter arboriphilus
17Met Lys Tyr Lys Leu Leu Glu Asn Pro Asp Ile Glu Glu Ala Tyr Asp1
5 10 15Leu Ile Asp Ser Gly Ile Arg Lys Lys Ala Val Ile Asn Ile Phe
Ala 20 25 30Tyr Cys Lys Val Leu Tyr Glu Gly Arg Ala Leu Ser Gln Leu
Asp Trp 35 40 45Gly Glu Arg Phe Ile Met Leu Lys Pro Asp Gly Ser Phe
Leu Val His 50 55 60Gln Glu Arg Lys Ile Asp Pro Val Asn Trp Gln Pro
Pro Lys Ser Arg65 70 75 80His Arg Ala Leu Ile Lys Glu Asn Asn Leu
Ile Leu Glu Ser His Arg 85 90 95Arg Thr Pro Lys Glu Lys Leu Glu Val
Glu Ile Glu Lys Val His Phe 100 105 110Ala Ser Phe Ala Leu Ala Glu
Asp Tyr Gln Glu Leu Glu Leu Ala Gly 115 120 125Tyr Glu Lys Asp Met
Gly Asp Met Ile Ile Lys His Pro Asn Met Ile 130 135 140Glu Gln Gly
Phe Thr Pro Thr Ala Arg Glu Tyr Asn Thr Glu His Gly145 150 155
160Phe Ile Asp Ile Leu Gly Lys Asp Ile Glu Gly Asn Ile Val Val Val
165 170 175Glu Leu Lys Cys Arg Lys Ala Gly Thr Asn Ala Val Lys Gln
Ile Arg 180 185 190Arg Tyr Leu Lys Asp Phe Glu Glu Asn Asp Asn Asp
Tyr Leu Lys Glu 195 200 205Ile Lys Ser Lys Lys Lys Lys Ile Arg Gly
Leu Leu Val Ala Pro Asp 210 215 220Ile Asn Glu Asp Ala Lys Glu Leu
Leu Glu Glu Glu Gly Ile Glu Phe225 230 235 240Lys Ala Val Asp Pro
Pro Lys Glu Leu Lys Ser Asp Lys Lys Val Thr 245 250 255Leu Asp Ile
Phe 26018261PRTMethanobrevibacter arboriphilus 18Met Lys Tyr Lys
Leu Leu Glu Asn Pro Asp Ile Glu Glu Thr Tyr Asp1 5 10 15Leu Ile Asp
Ser Gly Ile Arg Lys Lys Ala Val Ile Asn Ile Phe Ala 20 25 30Tyr Cys
Lys Val Leu Tyr Glu Gly Arg Ala Leu Ser Gln Leu Asp Trp 35 40 45Gly
Gly Glu Arg Phe Ile Met Leu Lys Pro Asp Gly Ser Phe Leu Val 50 55
60His Gln Glu Arg Lys Ile Asp Pro Val Asn Trp Gln Pro Pro Lys Ser65
70 75 80Arg His Arg Ala Leu Ile Lys Glu Asn Asn Leu Ile Leu Glu Ser
His 85 90 95Arg Arg Thr Pro Lys Glu Lys Leu Glu Val Glu Ile Glu Lys
Val His 100 105 110Phe Ala Ser Phe Ala Leu Ala Glu Asp Tyr Gln Glu
Leu Glu Leu Ala 115 120 125Gly Tyr Glu Lys Asp Met Gly Asp Met Ile
Ile Lys His Pro Asn Met 130 135 140Ile Glu Gln Gly Phe Thr Pro Thr
Ala Arg Glu Tyr Asn Thr Glu His145 150 155 160Gly Phe Ile Asp Ile
Leu Gly Lys Asp Ile Glu Gly Asn Ile Val Val 165 170 175Val Glu Leu
Lys Cys Arg Lys Ala Gly Thr Asn Ala Val Lys Gln Ile 180 185 190Arg
Arg Tyr Leu Lys Asp Phe Glu Glu Asn Asp Asn Asp Tyr Leu Lys 195 200
205Glu Ile Lys Ser Lys Lys Lys Lys Ile Arg Gly Leu Leu Val Ala Pro
210 215 220Asp Ile Asn Glu Asp Ala Lys Glu Leu Leu Glu Glu Glu Gly
Ile Glu225 230 235 240Phe Lys Ala Val Asp Pro Pro Lys Glu Leu Lys
Ser Asp Lys Lys Val 245 250 255Thr Leu Asp Ile Phe
26019260PRTMethanobrevibacter arboriphilus 19Met Lys Tyr Lys Leu
Leu Glu Asn Pro Asp Ile Glu Glu Thr Tyr Asp1 5 10 15Leu Ile Asp Ser
Gly Ile Arg Lys Lys Ala Val Ile Asn Ile Phe Ala 20 25 30Tyr Cys Lys
Val Leu Tyr Glu Gly Arg Ala Leu Ser Gln Leu Asp Trp 35 40 45Gly Glu
Arg Phe Ile Met Leu Lys Pro Asp Gly Ser Phe Leu Val His 50 55 60Gln
Glu Arg Lys Ile Asp Pro Val Asn Trp Gln Pro Pro Lys Ser Arg65 70 75
80His Arg Ala Leu Ile Lys Glu Asn Asn Leu Ile Leu Glu Ser His Arg
85 90 95Arg Thr Pro Lys Glu Lys Leu Glu Val Glu Ile Glu Lys Val His
Phe 100 105 110Ala Ser Phe Ala Leu Ala Glu Asp Tyr Gln Glu Leu Glu
Leu Ala Gly 115 120 125Tyr Glu Lys Asp Met Gly Asp Met Ile Ile Lys
His Pro Asn Met Ile 130 135 140Glu Gln Gly Phe Thr Pro Thr Ala Arg
Glu Tyr Asn Thr Glu His Gly145 150 155 160Phe Ile Asp Ile Leu Gly
Lys Asp Ile Glu Gly Asn
Ile Val Val Val 165 170 175Glu Leu Lys Cys Arg Lys Ala Gly Ile Asn
Ala Val Lys Gln Ile Arg 180 185 190Arg Tyr Leu Lys Asp Phe Glu Glu
Asn Asp Asn Asp Tyr Leu Lys Glu 195 200 205Val Lys Ser Lys Lys Lys
Lys Ile Arg Gly Leu Leu Val Ala Pro Asp 210 215 220Ile Asn Glu Asp
Ala Lys Glu Leu Leu Glu Glu Glu Gly Ile Glu Phe225 230 235 240Lys
Ala Val Asp Pro Pro Lys Glu Leu Arg Ser Asp Lys Lys Val Thr 245 250
255Leu Asp Ile Phe 26020259PRTMethanobrevibacter wolinii 20Met Asn
Tyr Lys Asn Ile Glu Lys Pro Asn Ile Asn Glu Ser Tyr Asn1 5 10 15Phe
Ile Glu Glu Gly Leu Arg Lys Arg Ala Thr Ile Ser Leu Tyr Thr 20 25
30Tyr Cys Lys Val Glu Tyr Glu Gly Arg Ala Leu Ser Gln Leu Asn Tyr
35 40 45Gly Glu Arg Leu Ile Ile Ile Lys Pro Asp Gly Ser Phe Leu Ile
His 50 55 60Gln Asn Lys Lys Val Glu Pro Val Asn Trp Gln Pro Pro Lys
Ser Arg65 70 75 80Thr Lys Val Tyr Ile Lys Asn Asn Lys Leu Phe Leu
Glu Ser Asn Arg 85 90 95Lys Thr Pro Arg Glu Arg Leu Glu Val Glu Ile
Asn Asn Ile Glu Thr 100 105 110Gly Thr Tyr Ala Ile Leu Glu Asp Tyr
Glu Glu Leu Glu Gln Ala Gly 115 120 125Tyr Glu Lys Asp Met Gly Asp
Met Ile Met Lys Asn Pro His Ile Ile 130 135 140Glu Glu Gly Phe Lys
Pro Ser Ser Arg Glu Tyr Ser Val Asn Ser Gly145 150 155 160Phe Ile
Asp Ile Leu Gly Lys Asp Lys Asn Gly Asn Leu Met Val Leu 165 170
175Glu Leu Lys Ser Arg Lys Ile Gly Val Ser Ala Val Lys Gln Ile Arg
180 185 190Arg Tyr Val Glu Asp Leu Lys Asn Thr Glu Asn Lys Glu Phe
Glu Thr 195 200 205Asp Asn Asn Lys Lys Arg Ile Arg Gly Ile Leu Val
Ala Pro Lys Ile 210 215 220Asp Asn Asp Ala Lys Glu Met Ile Glu Glu
Glu Asn Phe Glu Phe Val225 230 235 240Ala Cys Glu Pro Pro Lys Glu
Leu Lys Lys Asp Lys Lys Val Thr Leu 245 250 255Asp Leu
Phe21249PRTMethanothermobacter wolfeii 21Met Lys Cys Gln Val Phe
Glu Asn Pro Ser Pro Lys Lys Ala Tyr Arg1 5 10 15Val Ile Glu Glu Gly
Ile Arg Lys Arg Val Leu Ile Val Ile Leu Ala 20 25 30Cys Cys Ser Ala
Ser Tyr Glu Gly Arg Ala Arg Ser Arg Leu Glu Pro 35 40 45Gly Glu Arg
Leu Ile Val Ile Lys Pro Asp Gly Thr Phe Met Ile His 50 55 60Gln Asp
Arg Lys Val Asp Pro Val Asn Trp Gln Pro Pro Arg Ser Arg65 70 75
80Cys Arg Ser Tyr Met Lys Gly Gly Lys Leu Tyr Leu Glu Ser Ile Arg
85 90 95Arg Ser Pro Glu Glu Arg Leu Glu Val Glu Ile His Glu Ala His
Leu 100 105 110Val Ser Cys Tyr Thr Ala Arg Asp Arg His Glu Leu Glu
Val Ala Gly 115 120 125His Glu Arg Asp Met Gly Asp Met Ile Met Glu
His Pro His Leu Ile 130 135 140Glu Lys Gly Phe Arg Pro Val Ala Arg
Glu Tyr Ser Thr Asp Ala Gly145 150 155 160Phe Ile Asp Ile Leu Gly
Lys Asp Glu Asn Gly Ser Leu Val Ile Ile 165 170 175Glu Leu Lys Ser
Arg Lys Ala Gly Val Ser Ala Val Lys Gln Leu Lys 180 185 190Arg Tyr
Leu Asp Glu Phe Arg Asp Asp Arg Arg Gly Val Arg Gly Met 195 200
205Leu Val Ala Pro Ser Ile Thr His Asp Ala Met Glu Met Leu Glu Asp
210 215 220Glu Gly Leu Glu Phe Arg Ser Leu Glu Pro Leu Arg Glu Leu
Arg Ser225 230 235 240Ala Arg Gly Val Thr Leu Asp Asn Phe
24522249PRTMethanobacteriaceae archaeon 22Met Arg Phe Ile Ser Leu
Glu Asn Pro Thr Ser Glu Glu Ser Cys Arg1 5 10 15Ile Ile Lys Glu Gly
Leu Arg Lys Lys Ala Met Ile Ile Ile Phe Ser 20 25 30Cys Cys Lys Val
Arg Tyr Tyr Gly Arg Ala Lys Ser Arg Leu Gly Pro 35 40 45Gly Glu Arg
Leu Ile Ile Ile Lys Pro Asp Gly Ser Phe Leu Ile His 50 55 60Gln Asp
Arg Lys Val Glu Pro Val Asn Trp Gln Pro Pro Gly Ser Lys65 70 75
80Ala Lys Val Lys Ile Glu Asp Gly Lys Ile Leu Val Glu Ser Ile Arg
85 90 95Arg Lys Pro Ala Glu Lys Leu Met Val Glu Met Glu Lys Val His
Thr 100 105 110Leu Ser Tyr Tyr Leu Val Lys Asp Val His Glu Leu Glu
Val Ala Gly 115 120 125His Glu Glu Asp Met Arg Arg Leu Ile Leu Glu
Ser Pro Asp Ile Ile 130 135 140Glu Lys Gly Phe Arg Pro Ile Thr Arg
Glu Tyr Gln Thr Ser Asn Gly145 150 155 160Phe Ile Asp Ile Leu Gly
Lys Asp Glu Asn Gly Ser Leu Met Val Leu 165 170 175Glu Leu Lys Ser
Arg Arg Ala Gly Val Ser Ala Val Arg Gln Leu Lys 180 185 190Arg Tyr
Leu Glu Asp Phe Lys Asp Asp Lys His Gly Val Arg Gly Val 195 200
205Leu Val Ala Pro Ser Ile Thr His Asp Ala Arg Glu Leu Leu Glu Ala
210 215 220Glu Gly Leu Glu Phe Lys Ser Leu Glu Pro Pro Arg Glu Leu
Lys Lys225 230 235 240Gly Tyr Lys Met Thr Leu Asp Lys Phe
24523249PRTMethanothermobacter thermautotrophicus 23Met Lys Cys Lys
Val Ser Glu Asn Pro Ser Ile Lys Glu Ala Tyr Arg1 5 10 15Leu Ile Glu
Asp Gly Ile Arg Lys Arg Ala Leu Val Val Ile Leu Ala 20 25 30Cys Cys
Ser Ala Ser Tyr Glu Gly Arg Ala Arg Ser Arg Leu Asp Ala 35 40 45Gly
Glu Arg Leu Ile Val Ile Lys Pro Asp Gly Thr Phe Met Val His 50 55
60Gln Asp Arg Lys Val Asp Pro Val Asn Trp Gln Pro Pro Lys Ser Arg65
70 75 80Ser Arg Ala Tyr Ile Lys Arg Gly Ser Leu Tyr Leu Glu Ser Ile
Arg 85 90 95Arg Asp Pro Glu Glu Arg Leu Glu Val Glu Ile His Glu Ala
His Leu 100 105 110Val Ser Tyr Tyr Leu Ala Arg Asp Val His Asp Leu
Met Val Ala Gly 115 120 125His Glu Asn Asp Met Gly Asp Met Ile Ile
Met His Pro His Leu Ile 130 135 140Glu Lys Gly Phe Arg Pro Val Ala
Arg Glu Tyr Ala Val Thr Ser Gly145 150 155 160Phe Ile Asp Ile Leu
Gly Lys Asp Glu Asn Gly Ser Leu Met Ile Ile 165 170 175Glu Leu Lys
Ser Arg Lys Ala Gly Val Ser Ala Val Lys Gln Leu Lys 180 185 190Arg
Tyr Val Asp Glu Phe Arg Glu Asp Arg Val Gly Val Arg Gly Val 195 200
205Leu Val Ala Pro Ser Ile Thr His Asp Ala Met Glu Met Leu Glu Glu
210 215 220Glu Gly Leu Glu Phe Arg Glu Ile Glu Pro Pro Arg Glu Leu
Arg Ser225 230 235 240Asn Arg Gly Val Thr Leu Asp Asn Phe
24524249PRTMethanothermobacter thermautotrophicus str. Delta H
24Met Lys Cys Lys Val Ser Glu Asn Pro Ser Ile Lys Glu Ala Tyr Arg1
5 10 15Leu Ile Glu Asp Gly Ile Arg Lys Arg Ala Leu Val Val Ile Leu
Ala 20 25 30Cys Cys Ser Ala Ser Tyr Glu Gly Arg Ala Arg Ser Arg Leu
Asp Ala 35 40 45Gly Glu Arg Leu Ile Val Ile Lys Pro Asp Gly Thr Phe
Met Val His 50 55 60Gln Asp Arg Lys Val Asp Pro Val Asn Trp Gln Pro
Pro Lys Ser Arg65 70 75 80Ser Arg Ala Tyr Ile Lys Arg Gly Ser Leu
Tyr Leu Glu Ser Ile Arg 85 90 95Arg Asp Pro Glu Glu Arg Leu Glu Val
Glu Ile His Glu Ala His Leu 100 105 110Val Ser Tyr Tyr Leu Ala Arg
Asp Val His Asp Leu Met Val Ala Gly 115 120 125His Glu Asn Asp Met
Gly Asp Met Ile Ile Met His Pro His Leu Ile 130 135 140Glu Lys Gly
Phe Arg Pro Val Ala Arg Glu Tyr Ala Val Thr Ser Gly145 150 155
160Phe Ile Asp Ile Leu Gly Lys Asp Glu Asn Gly Ser Leu Met Ile Ile
165 170 175Glu Leu Lys Ser Arg Lys Ala Gly Val Ser Ala Val Lys Gln
Leu Lys 180 185 190Arg Tyr Val Asp Glu Phe Arg Glu Asp Arg Val Gly
Val Arg Gly Val 195 200 205Leu Val Ala Pro Ser Ile Thr His Asp Ala
Met Glu Met Leu Glu Glu 210 215 220Glu Gly Leu Glu Phe Arg Glu Ile
Glu Pro Pro Arg Glu Leu Arg Ser225 230 235 240Asn Arg Gly Val Thr
Leu Asp Asn Phe 24525249PRTMethanothermobacter 25Met Lys Cys Arg
Val Ser Glu Asn Pro Ser Arg Asn Glu Ala Tyr Gln1 5 10 15Leu Leu Glu
Glu Gly Ile Arg Lys Arg Ser Leu Ile Val Ile Leu Ala 20 25 30Cys Cys
Ser Ala Ser Tyr Glu Gly Arg Ala Arg Ser Ser Leu Gly Ala 35 40 45Gly
Glu Arg Leu Ile Val Ile Lys Pro Asp Gly Thr Phe Met Val His 50 55
60Gln Asp Arg Lys Val Asp Pro Val Asn Trp Gln Pro Pro Arg Ser Arg65
70 75 80Cys Arg Val Tyr Met Lys Arg Gly Ser Leu Phe Leu Glu Ser Ile
Arg 85 90 95Arg Ser Pro Glu Glu Arg Leu Glu Val Glu Leu His Glu Ile
His Leu 100 105 110Ile Ser Ser Tyr Leu Pro Arg Asp Met His Glu Leu
Thr Val Ser Gly 115 120 125His Glu Ser Asp Met Gly Asp Met Ile Ile
Met His Pro His Leu Ile 130 135 140Glu Pro Gly Phe Arg Pro Val Ala
Arg Glu Tyr Ala Thr Ser Ser Gly145 150 155 160Phe Ile Asp Ile Leu
Gly Lys Asp Glu Asn Gly Ser Leu Met Ile Ile 165 170 175Glu Leu Lys
Ser Arg Lys Ala Gly Val Ser Ala Val Lys Gln Leu Arg 180 185 190Arg
Tyr Val Asp Glu Phe Arg Asp Asp Arg Val Gly Val Arg Gly Ile 195 200
205Leu Val Ala Pro Ser Ile Thr His Asp Ala Leu Glu Met Leu Glu Asp
210 215 220Glu Gly Leu Glu Phe Arg Glu Ile Glu Pro Pro Arg Glu Leu
Lys Ser225 230 235 240Asn Arg Gly Val Thr Leu Asp Asn Phe
24526248PRTMethanothermobacter 26Arg Phe Lys Thr Ile Glu Asn Pro
Ser Pro Lys Glu Ser His Glu Leu1 5 10 15Ile Ser Glu Gly Leu Arg Lys
Lys Ala Met Ile Ile Leu Phe Ala Cys 20 25 30Cys Lys Val Gln Tyr His
Gly Arg Ala Lys Ser Arg Leu Gly Thr Gly 35 40 45Glu Arg Leu Ile Ile
Ile Lys Pro Asp Gly Ser Phe Leu Ile His Gln 50 55 60Asp Arg Lys Val
Asp Pro Val Asn Trp Gln Pro Pro Gly Ser Lys Thr65 70 75 80Lys Ile
Lys Ile Glu Asn Gly Lys Ile Leu Leu Glu Ser Arg Arg Arg 85 90 95Lys
Pro Pro Glu Lys Leu Thr Ala Glu Ile Lys Arg Ile His Ser Leu 100 105
110Ser Tyr His Leu Ala Arg Asp Ala His Glu Leu Gln Val Thr Gly His
115 120 125Glu Glu Asp Met Arg Gln Leu Ile Leu Glu Ser Pro Asp Ile
Ile Glu 130 135 140Lys Gly Phe Arg Pro Thr Thr Ser Glu Tyr Pro Thr
Ser Asn Gly Phe145 150 155 160Ile Asp Ile Leu Gly Lys Asp Lys Asn
Gly Ser Leu Met Ile Leu Glu 165 170 175Leu Lys Ser Arg Arg Ala Gly
Leu Asn Ala Val Arg Gln Leu Lys Arg 180 185 190Tyr Leu Lys Asp Phe
Lys Asp Asp Lys His Gly Val Arg Gly Val Leu 195 200 205Val Ala Pro
Ser Ile Thr His Asp Ala Lys Glu Leu Leu Glu Lys Glu 210 215 220Gly
Leu Glu Phe Lys Ser Leu Lys Pro Pro Gln Glu Leu Lys Lys Asp225 230
235 240His Lys Ile Thr Leu Asp Lys Phe
24527249PRTMethanothermobacter marburgensis 27Met Lys Cys Arg Val
Ser Glu Asn Pro Ser Arg Ser Glu Ala Tyr Arg1 5 10 15Leu Leu Glu Glu
Gly Ile Arg Lys Arg Ser Leu Ile Val Ile Leu Ala 20 25 30Cys Cys Ser
Ala Ser Tyr Glu Gly Arg Ala Arg Ser Ser Leu Gly Ala 35 40 45Gly Glu
Arg Leu Ile Val Ile Lys Pro Asp Gly Thr Phe Met Val His 50 55 60Gln
Asp Arg Lys Val Asp Pro Val Asn Trp Gln Pro Pro Arg Ser Arg65 70 75
80Cys Arg Val Tyr Met Lys Gln Lys Ser Leu Phe Leu Glu Ser Ile Arg
85 90 95Arg Ser Pro Glu Glu Arg Leu Glu Val Glu Leu His Glu Val His
Leu 100 105 110Ile Ser Phe Tyr Leu Pro Arg Asp Met His Glu Leu Thr
Val Ser Gly 115 120 125His Glu Ser Asp Met Gly Asp Met Ile Ile Met
His Pro His Leu Ile 130 135 140Glu Pro Gly Phe Arg Pro Val Ala Arg
Glu Tyr Ala Thr Ser Ser Gly145 150 155 160Phe Ile Asp Ile Leu Gly
Lys Asp Glu Asn Gly Ser Leu Met Ile Ile 165 170 175Glu Leu Lys Ser
Arg Lys Ala Gly Val Ser Ala Val Lys Gln Leu Arg 180 185 190Arg Tyr
Val Asp Glu Phe Arg Asp Asp Gln Val Gly Val Arg Gly Ile 195 200
205Leu Val Ala Pro Ser Ile Thr His Asp Ala Leu Glu Met Leu Glu Asp
210 215 220Glu Gly Leu Glu Phe Arg Glu Ile Glu Pro Pro Arg Glu Leu
Lys Ser225 230 235 240Asn Arg Gly Val Thr Leu Asp Asn Phe
24528249PRTMethanobacterium 28Met Ile Ile Lys Leu Asp Glu Asn Pro
Gly Thr Glu Glu Val Phe Asp1 5 10 15Phe Ile Asn Glu Ala Ile Ser Lys
Arg Ala Phe Ile Ile Ile Val Ala 20 25 30Cys Cys Arg Ile Lys Tyr Arg
Gly Arg Ala Thr Ser Arg Leu Gly Ser 35 40 45Gly Asp Arg Thr Ile Ile
Ile Lys Thr Asp Gly Ser Phe Leu Val His 50 55 60Gln Asp Tyr Asn Leu
Glu Pro Val Asn Trp Gln Pro Pro Asn Cys Lys65 70 75 80Phe Lys Thr
Arg Met Glu Asn Gly Arg Val Tyr Leu Cys Gly Val Arg 85 90 95Arg Asn
Pro Pro Glu Ser Leu Glu Val Glu Ile Cys Lys Thr His Met 100 105
110Ile Ser Tyr His Ile Gly Lys Asp Ile Lys Lys Leu Glu Leu Ala Gly
115 120 125Tyr Glu Glu Asp Met Arg Ala Met Ile Met Glu Ser Pro Glu
Leu Ile 130 135 140Glu Lys Gly Phe Arg Pro Thr Ser Lys Glu Tyr Pro
Val Ser Thr Gly145 150 155 160Phe Ile Asp Ile Leu Gly Lys Asp Lys
Asn Gly Ser Leu Met Val Leu 165 170 175Glu Leu Lys Ser Arg Gln Ala
Gly Ile Asn Ala Val Lys Gln Leu Lys 180 185 190Lys Tyr Phe Glu Asp
Phe Thr Asp His Lys Asp Phe Val Arg Gly Leu 195 200 205Leu Val Ala
Pro Ser Ile Thr Glu Asp Ala Gln Glu Leu Leu Glu Lys 210 215 220Tyr
Gln Leu Glu Phe Lys Ala Leu Glu Pro Pro Lys Glu Leu Lys Ser225 230
235 240Ala Lys Ser Val Thr Leu Asp Phe Phe
24529249PRTMethanobacterium paludis 29Met Lys Phe Leu Ser Glu Lys
Asn Pro Asp Ile Lys Arg Thr Tyr Glu1 5 10 15Ile Ile Asn Glu Gly Ile
Ser Lys Arg Ala Val Ile Val Ile Met Ala 20 25 30Cys Cys Ser Val Leu
Tyr Glu Gly Arg Ala Arg Ser Arg Leu Ala Asp 35 40 45Gly Asp Arg Met
Val Met Ile Lys Thr Asp Gly Ser Phe Leu Val His 50 55 60Gln Asp Arg
Asn Leu Glu Pro Val Asn Trp Gln Pro Pro Lys Ser Gln65 70 75 80Cys
Lys Ala Ser Leu Lys Glu Gly Leu Leu His Ile Glu Gly Ala Arg 85
90
95Arg Asn Pro Pro Glu Arg Leu Glu Val Glu Ile His Ser Thr Tyr Met
100 105 110Ala Ser Tyr Phe Ile Gly Glu Asp Ser Lys Asp Leu Glu Leu
Ala Gly 115 120 125Tyr Glu Ala Asp Met Val Asp Met Ala Phe Glu Ser
Pro Glu Leu Ile 130 135 140Glu Lys Gly Phe Arg Pro Thr Ser Arg Glu
Tyr Ser Thr Glu Asn Gly145 150 155 160Phe Ile Asp Ile Leu Gly Lys
Asp Gln Asp Gly Asn Leu Met Val Leu 165 170 175Glu Phe Lys Ser Arg
Arg Ala Gly Ile Asn Ala Val Lys Gln Leu Lys 180 185 190Arg Tyr Leu
Asp Cys Phe Ala Asp His Lys Gln Phe Val Arg Gly Leu 195 200 205Leu
Val Ala Pro Ser Val Thr Asp Asp Ala Ser Asp Leu Leu Lys Glu 210 215
220Tyr Lys Leu Glu Phe Lys Glu Leu Glu Pro Pro Met Glu Phe Asp
Gly225 230 235 240Asp Lys Asn Leu Thr Leu Asp Phe Phe
24530249PRTMethanobacterium 30Met Ile Ile Lys Leu Asp Glu Asn Pro
Gly Thr Glu Glu Val Phe Asp1 5 10 15Phe Ile Asn Glu Ala Ile Ser Lys
Arg Ala Phe Ile Ile Ile Val Ala 20 25 30Cys Cys Arg Ile Lys Tyr Arg
Gly Arg Ala Thr Ser Arg Leu Gly Ser 35 40 45Gly Asp Arg Thr Ile Ile
Ile Lys Thr Asp Gly Ser Phe Leu Val His 50 55 60Gln Asp Tyr Asn Leu
Glu Pro Val Asn Trp Gln Pro Pro Asn Cys Lys65 70 75 80Phe Lys Thr
Arg Met Glu Asn Asp Arg Val Tyr Ile Cys Gly Val Arg 85 90 95Arg Asn
Pro Pro Glu Ser Leu Glu Val Glu Ile Cys Lys Thr His Met 100 105
110Ile Ser Tyr His Ile Gly Lys Asp Ile Lys Lys Leu Glu Leu Ala Gly
115 120 125Tyr Glu Glu Asp Leu Arg Thr Met Ile Met Glu Ser Pro Glu
Leu Ile 130 135 140Glu Lys Gly Phe Arg Pro Thr Ser Lys Glu Tyr Gln
Val Ser Thr Gly145 150 155 160Phe Ile Asp Ile Leu Gly Lys Asp Lys
Asn Gly Ser Leu Met Ile Leu 165 170 175Glu Leu Lys Ser Arg Gln Ala
Gly Val Asn Ala Val Lys Gln Leu Lys 180 185 190Lys Tyr Phe Glu Asp
Phe Thr Asp His Lys Asp Phe Val Arg Gly Leu 195 200 205Leu Val Ala
Pro Ser Val Thr Glu Asp Ala Gln Glu Leu Leu Glu Lys 210 215 220Tyr
Gln Leu Glu Phe Lys Ala Leu Glu Pro Pro Lys Glu Leu Lys Ser225 230
235 240Ala Lys Ser Val Thr Leu Asp Phe Phe 24531248PRTPalaeococcus
ferrophilus 31Met Lys Leu Arg Ala Leu Glu Asn Pro Ser Ala Glu Glu
Leu Glu Ser1 5 10 15Ile Ile Ser Glu Gly Leu Ser Ser Glu Ala Ile Ile
Thr Ile Phe Ala 20 25 30Arg Cys Arg Val Tyr Tyr Asp Gly Arg Ala Lys
Ser Glu Leu Gly Glu 35 40 45Gly Asp Arg Val Ile Leu Ile Lys Pro Asp
Gly Ser Phe Leu Ile His 50 55 60Gln Arg Asn Lys Arg Glu Pro Val Asn
Trp Gln Pro Pro Gly Ser Ser65 70 75 80Val Phe Phe Glu Arg Glu Lys
Leu Arg Leu Val Ser Val Arg Arg Lys 85 90 95Pro Arg Glu Thr Leu Glu
Val Glu Leu Leu Lys Val Tyr Leu Ala Thr 100 105 110Tyr Phe Gln Ala
Glu Asp Ser Glu Glu Leu Thr Leu Ile Gly Ser Glu 115 120 125Ala Glu
Met Arg Asp Tyr Ile Phe Asp His Pro Glu Val Ile Glu Glu 130 135
140Gly Phe Lys Pro Leu Phe Arg Glu Lys Pro Ile Lys His Gly Ile
Val145 150 155 160Asp Ile Phe Gly Arg Asp Arg Asp Gly Asn Ile Val
Ile Leu Glu Leu 165 170 175Lys Arg Arg Arg Ala Asp Leu His Ala Val
Ser Gln Leu Lys Arg Tyr 180 185 190Val Glu Asp Met Arg Glu Glu Tyr
Glu Asn Val Arg Gly Ile Leu Val 195 200 205Ala Pro Ser Leu Thr Ser
Gly Ala Lys Lys Leu Leu Glu Lys Glu Gly 210 215 220Leu Glu Phe Arg
Lys Val Lys Pro Pro Lys Gly Glu Arg Lys Lys Gly225 230 235 240Lys
Gln Lys Thr Leu Asp Ser Phe 24532243PRTMethanobacterium 32Lys Asn
Pro Asp Thr Gln Arg Val Leu Glu Ile Ile Asn Glu Gly Leu1 5 10 15Ser
Lys Arg Ala Val Ile Thr Ile Met Ala Cys Cys Arg Val Asp Tyr 20 25
30Asn Gly Arg Ala Val Ser Arg Leu Gly Leu Gly Asp Arg Ile Ile Ile
35 40 45Ile Lys Ala Asp Gly Ser Phe Leu Ile His Gln Asp Arg Asn Leu
Glu 50 55 60Pro Val Asn Trp Gln Pro Pro Lys Thr Lys Val Ser Ala Asp
Ile Tyr65 70 75 80Gln Gly Met Val Lys Ile Lys Gly Val Arg Arg Asn
Pro His Glu Ser 85 90 95Leu Glu Val Lys Ile Leu Gln Thr His Met Ile
Ser Tyr Phe Ile Gly 100 105 110Glu Asp Ser Glu Asn Leu Glu Leu Ala
Gly Tyr Glu Ala Asp Met Gly 115 120 125Asp Leu Ile Phe Lys Asp Pro
Asp Val Phe Glu Lys Gly Phe Arg Pro 130 135 140Thr Ser Arg Glu Tyr
His Thr Pro Gln Gly Phe Ile Asp Ile Leu Gly145 150 155 160Lys Asp
Gln Asp Gly Asn Ile Thr Ile Leu Glu Leu Lys Ser Arg Lys 165 170
175Ala Gly Val Asn Ala Val Lys Gln Leu Arg Arg Tyr Leu Asp Cys Phe
180 185 190Ser Asp His Lys Glu Lys Val Arg Gly Val Leu Val Ala Pro
Ser Ala 195 200 205Thr Asp Asp Ala Leu Glu Leu Leu Glu Lys Gln Gly
Met Glu Phe Lys 210 215 220Ala Leu Glu Pro Pro Arg Glu Leu Asn Asn
Glu Lys Val Val Thr Leu225 230 235 240Glu Asn Phe33244PRTPyrococcus
abyssi 33Ile Lys Glu Asn Pro Ser Glu Glu Glu Ile Lys Glu Leu Leu
Asp Leu1 5 10 15Ala Glu Lys His Gly Gly Val Val Thr Ile Phe Ala Arg
Cys Lys Val 20 25 30His Tyr Glu Gly Arg Ala Lys Ser Glu Leu Gly Glu
Gly Asp Arg Ile 35 40 45Ile Ile Ile Lys Pro Asp Gly Ser Phe Leu Ile
His Gln Asn Lys Lys 50 55 60Arg Glu Pro Val Asn Trp Gln Pro Pro Gly
Ser Lys Val Thr Phe Lys65 70 75 80Glu Asn Ser Ile Ile Ser Ile Arg
Arg Arg Pro Tyr Glu Arg Leu Glu 85 90 95Val Glu Ile Ile Glu Pro Tyr
Ser Leu Val Val Phe Leu Ala Glu Asp 100 105 110Tyr Glu Glu Leu Ala
Leu Thr Gly Ser Glu Ala Glu Met Ala Asn Leu 115 120 125Ile Phe Glu
Asn Pro Arg Val Ile Glu Glu Gly Phe Lys Pro Ile Tyr 130 135 140Arg
Glu Lys Pro Ile Arg His Gly Ile Val Asp Val Met Gly Val Asp145 150
155 160Lys Asp Gly Asn Ile Val Val Leu Glu Leu Lys Arg Arg Lys Ala
Asp 165 170 175Leu His Ala Val Ser Gln Leu Lys Arg Tyr Val Asp Ser
Leu Lys Glu 180 185 190Glu Tyr Gly Glu Asn Val Arg Gly Ile Leu Val
Ala Pro Ser Leu Thr 195 200 205Glu Gly Ala Lys Lys Leu Leu Glu Lys
Glu Gly Leu Glu Phe Arg Lys 210 215 220Leu Glu Pro Pro Lys Lys Gly
Asn Glu Lys Arg Ser Lys Gln Lys Thr225 230 235 240Leu Asp Phe
Phe34249PRTMethanobacterium congolense 34Met Lys Phe Leu Ser Glu
Lys Asn Pro Asp Ile His Arg Thr Tyr Glu1 5 10 15Ile Ile Asn Glu Gly
Ile Ser Lys Arg Ala Phe Val Val Leu Met Ala 20 25 30Cys Cys Lys Val
Leu Tyr Gln Gly Arg Ala Lys Ser Arg Leu Gly Ser 35 40 45Gly Asp Arg
Phe Ile Ile Ile Lys Pro Asp Gly Ser Phe Met Val His 50 55 60Gln Asp
Arg Asn Leu Glu Pro Val Asn Trp Gln Pro Pro Lys Ser Asn65 70 75
80Cys Lys Ala Ser Leu Glu Asn Gly Ile Leu Gln Ile Thr Gly Ser Arg
85 90 95Arg Asn Pro Pro Glu Ser Leu Glu Val Glu Ile His Cys Thr Tyr
Met 100 105 110Ala Ser Tyr Phe Ile Gly Glu Asp Ser Lys Glu Leu Glu
Ile Ala Gly 115 120 125Tyr Glu Glu Asn Met Arg Glu Met Val Phe Glu
Ser Pro Glu Leu Ile 130 135 140Glu Glu Gly Phe Arg Pro Thr Ser Arg
Glu Tyr Gln Thr Glu Asn Gly145 150 155 160Phe Ile Asp Ile Leu Gly
Lys Asp Lys Asn Gly Ala Leu Met Val Leu 165 170 175Glu Leu Lys Ser
Arg Arg Ala Gly Val Asn Ala Val Lys Gln Leu Lys 180 185 190Lys Tyr
Leu Asp Cys Phe Thr Asp His Lys Glu Phe Val Arg Gly Val 195 200
205Leu Val Ala Pro Ser Val Thr Asp Asp Ala Met Glu Leu Leu Lys Glu
210 215 220Tyr Gln Leu Glu Phe Lys Glu Leu His Pro Pro Met Glu Leu
Gly Gly225 230 235 240Gly Lys Asn Leu Thr Leu Asp Phe Phe
24535249PRTMethanobacterium 35Met Lys Phe Leu Ser Glu Lys Asn Pro
Asp Gln Gln Arg Thr Phe Glu1 5 10 15Ile Ile Asn Glu Gly Leu Ser Lys
Lys Ala Val Ile Val Leu Met Val 20 25 30Cys Cys Arg Val Ile Tyr Glu
Gly Arg Ala Arg Ser Lys Leu Ala Ser 35 40 45Gly Asp Arg Met Ile Ile
Ile Lys Ser Asp Gly Ser Phe Met Ile His 50 55 60Gln Asp Arg Asn Leu
Glu Pro Val Asn Trp Gln Pro Pro Lys Ser Gln65 70 75 80Cys Lys Ala
Ser Leu Lys Lys Gly Ile Met Tyr Ile Glu Gly Lys Arg 85 90 95Arg Asn
Pro Pro Glu Arg Leu Glu Val Glu Ile His Asn Thr Tyr Ile 100 105
110Ala Ser Tyr Phe Asn Gly Glu Asp Ser Lys Asp Leu Glu Leu Thr Gly
115 120 125Tyr Glu Glu Asn Met Arg Glu Leu Val Phe Glu Asn Pro Glu
Ile Ile 130 135 140Glu Glu Gly Phe Arg Pro Ser Asn Arg Glu Tyr Ser
Thr Pro Asn Gly145 150 155 160Phe Ile Asp Val Leu Gly Lys Asp Lys
Asn Gly Asn Leu Met Val Ile 165 170 175Glu Leu Lys Ser Arg Arg Ala
Gly Ile Asn Ala Val Lys Gln Leu Lys 180 185 190Lys Tyr Leu Asp Cys
Phe Ser Asp His Lys Glu Phe Val Arg Gly Ile 195 200 205Leu Val Ala
Pro Ser Ile Thr Gly Asp Ala Glu Glu Leu Leu Glu Glu 210 215 220Tyr
Lys Leu Glu Tyr Lys Ser Leu Glu Pro Pro Arg Glu Phe Gly Asn225 230
235 240Asp Lys Asn Leu Thr Leu Asp Phe Phe
24536242PRTMethanobacterium 36Asn Pro Asp Thr Glu Leu Val Leu Glu
Ile Ile Thr Glu Gly Ile Ser1 5 10 15Lys Arg Ala Phe Ile Thr Ile Met
Ala Ser Cys Arg Val Tyr Tyr Glu 20 25 30Gly Arg Ala Thr Ser Arg Leu
Glu Leu Gly Asp Arg Ile Ile Leu Ile 35 40 45Lys Ser Asp Gly Ser Phe
Ile Ile His Gln Asp Arg Asn Leu Glu Pro 50 55 60Val Asn Trp Gln Pro
Pro Lys Thr Lys Val Thr Val Asn Ile His Gln65 70 75 80Gly Met Val
Lys Leu Lys Gly Val Arg Arg Ser Pro Ser Glu Ser Leu 85 90 95Glu Val
Glu Ile Leu Gln Thr His Leu Ala Ser Tyr Phe Ile Gly Glu 100 105
110Asp Ser Glu Ser Leu Glu Leu Ala Gly Tyr Glu Ala Asp Met Gly Asp
115 120 125Leu Ile Phe Lys Asp Pro Glu Val Ile Glu Lys Gly Phe Arg
Pro Thr 130 135 140Ser Arg Glu Tyr His Thr Pro Gln Gly Phe Ile Asp
Ile Met Gly Lys145 150 155 160Asp His Glu Gly Asn Ile Thr Ile Leu
Glu Leu Lys Ser Arg Lys Ala 165 170 175Gly Thr Asn Ala Val Lys Gln
Leu Arg Arg Tyr Val Asp Cys Phe Cys 180 185 190Asp His Lys Glu Lys
Val Arg Gly Val Leu Val Ala Pro Ser Ala Thr 195 200 205Asp Asp Ala
Leu Glu Met Leu Glu Glu Gln Gly Met Glu Phe Lys Ala 210 215 220Leu
Glu Ala Pro Arg Glu Leu Lys Asn Asn Lys Ile Val Thr Leu Glu225 230
235 240Ser Phe37244PRTPyrococcus Abyssimisc_feature(84)..(84)Xaa
can be any naturally occurring amino
acidmisc_feature(125)..(125)Xaa can be any naturally occurring
amino acidmisc_feature(157)..(157)Xaa can be any naturally
occurring amino acidmisc_feature(183)..(183)Xaa can be any
naturally occurring amino acid 37Ile Lys Glu Asn Pro Ser Glu Glu
Glu Ile Lys Glu Leu Leu Asp Leu1 5 10 15Ala Glu Lys His Gly Gly Val
Val Thr Ile Phe Ala Arg Cys Lys Val 20 25 30His Tyr Glu Gly Arg Ala
Lys Ser Glu Leu Gly Glu Gly Asp Arg Ile 35 40 45Ile Ile Ile Lys Pro
Asp Gly Ser Phe Leu Ile His Gln Asn Lys Lys 50 55 60Arg Glu Pro Val
Asn Trp Gln Pro Pro Gly Ser Lys Val Thr Phe Lys65 70 75 80Glu Asn
Ser Xaa Ile Ser Ile Arg Arg Arg Pro Tyr Glu Arg Leu Glu 85 90 95Val
Glu Ile Ile Glu Pro Tyr Ser Leu Val Val Phe Leu Ala Glu Asp 100 105
110Tyr Glu Glu Leu Ala Leu Thr Gly Ser Glu Ala Glu Xaa Ala Asn Leu
115 120 125Ile Phe Glu Asn Pro Arg Val Ile Glu Glu Gly Phe Lys Pro
Ile Tyr 130 135 140Arg Glu Lys Pro Ile Arg His Gly Ile Val Asp Val
Xaa Gly Val Asp145 150 155 160Lys Asp Gly Asn Ile Val Val Leu Glu
Leu Lys Arg Arg Lys Ala Asp 165 170 175Leu His Ala Val Ser Gln Xaa
Lys Arg Tyr Val Asp Ser Leu Lys Glu 180 185 190Glu Tyr Gly Glu Asn
Val Arg Gly Ile Leu Val Ala Pro Ser Leu Thr 195 200 205Glu Gly Ala
Lys Lys Leu Leu Glu Lys Glu Gly Leu Glu Phe Arg Lys 210 215 220Leu
Glu Pro Pro Lys Lys Gly Asn Glu Lys Arg Ser Lys Gln Lys Thr225 230
235 240Leu Asp Phe Phe38249PRTMethanosphaera 38Met Lys Phe Lys Thr
Ile Glu Asn Pro Glu Asn Asn Asp Ala Tyr Thr1 5 10 15Leu Leu Gln Glu
Gly Phe Asp Lys Lys Ala Met Ile Ile Val Leu Ala 20 25 30Glu Cys His
Val Glu Tyr Glu Gly Arg Ala Arg Ser Arg Leu Asp Ile 35 40 45Gly Asp
Arg Leu Ile Leu Ile Lys Lys Asp Gly Thr Phe Val Ile His 50 55 60Gln
Glu Leu Asn Leu Asp Pro Val Asn Trp Gln Ala Pro Gly Cys Lys65 70 75
80Asn Lys Val Lys Leu Glu Asp Asn His Val Met Leu Ile Ser Lys Lys
85 90 95Thr Lys Pro Thr Glu Glu Ile Lys Val Phe Leu Asp Thr Ile Tyr
Asn 100 105 110Ile Thr Tyr Tyr Asn Cys Leu Asp Thr Lys Asn Leu Glu
Ile Arg Gly 115 120 125Tyr Glu Lys His Met Val Asp Leu Ala Trp Glu
Lys Pro Glu Leu Ile 130 135 140Glu Lys Gly Phe Arg Pro Thr Arg Arg
Glu Tyr Gln Thr Glu Asn Gly145 150 155 160Phe Ile Asp Leu Met Gly
Thr Asp Ile Asp Glu Lys Leu Met Ile Leu 165 170 175Glu Phe Lys Ser
Arg Lys Ala Gly Thr Asn Ala Val Lys Gln Leu Lys 180 185 190Gly Tyr
Ile Asp Cys Phe Lys Asp Asn Lys Glu Phe Val Arg Gly Ile 195 200
205Ile Val Ala Pro Asp Ile Thr Asp Asn Ala Arg Glu Leu Leu Glu Ser
210 215 220Leu Gln Met Glu Phe Ile Ser Met Asn Pro Pro Leu Asp Leu
Leu Lys225 230 235 240Gln Lys Ala Ser Thr Leu Asp Ser Phe
24539250PRTMethanobacterium 39Val Lys Phe Lys Val Glu Glu Asn Pro
Ser Ile Glu Lys Thr Asn Glu1 5 10 15Leu Leu Lys Asp Gly Leu Lys Asn
Lys Ala Ile Ile Ile Ile Thr Ala 20 25 30Cys Cys Arg Val Phe Tyr Glu
Gly Arg Ala Lys Ser Asn Leu Glu Leu 35 40
45Gly Asp Arg Val Ile Ile Ile Lys Pro Asp Gly Ser Phe Leu Ile His
50 55 60Lys Ser Glu Lys Arg Asn Pro Val Asn Trp Gln Pro Pro Gly Cys
Thr65 70 75 80Val Lys Phe Lys Val Lys Asp Asp Leu Met Leu Ile Arg
Ser Ile Arg 85 90 95Lys Asn Pro Lys Glu Ile Leu Asp Val Glu Ile Ser
Lys Thr Tyr Met 100 105 110Ala Thr Tyr Phe Val Ala Lys Asp Tyr Glu
Glu Leu Asp Leu Ile Gly 115 120 125Ser Glu Glu Asp Met Ala Asn Leu
Ile Phe Tyr Glu Asn Pro Glu Val 130 135 140Ile Glu Glu Gly Phe Lys
Pro Ile Ala Lys Glu Lys Ser Ile Ser Asn145 150 155 160Gly Val Ile
Asp Ile Leu Gly Lys Asp Lys Asn Gly Asn Ile Met Val 165 170 175Leu
Glu Leu Lys Arg Val Arg Gly Ser Leu Gly Ala Val Ser Gln Leu 180 185
190Lys Arg Tyr Val Asp Asn Leu Lys Glu Glu Asn Glu Gly Leu Arg Gly
195 200 205Met Leu Val Ala Pro Ser Ile Thr Asp Ser Ala Met Lys Leu
Leu Lys 210 215 220Glu Tyr Gly Leu Glu Phe Lys Glu Leu His Pro Pro
Lys Lys Leu Lys225 230 235 240Lys Glu Asp Ile Ile Lys Leu Asp Phe
Phe 245 25040250PRTMethanobacterium 40Val Lys Phe Lys Val Glu Glu
Asn Pro Asp Ile Glu Lys Thr Asn Glu1 5 10 15Ile Leu Lys Asp Gly Leu
Lys Asn Lys Ala Ile Ile Ile Ile Thr Ala 20 25 30Cys Cys Arg Val Phe
Tyr Glu Gly Arg Ala Lys Ser Asn Leu Glu Leu 35 40 45Gly Asp Arg Val
Ile Ile Ile Lys Pro Asp Gly Ser Phe Leu Ile His 50 55 60Lys Ser Glu
Lys Arg Asn Pro Val Asn Trp Gln Pro Pro Gly Cys Thr65 70 75 80Val
Lys Phe Lys Val Lys Asp Asp Leu Met Leu Ile Arg Ser Ile Arg 85 90
95Lys Asn Pro Lys Glu Ile Leu Asp Val Glu Ile Ser Lys Thr Tyr Met
100 105 110Ala Thr Tyr Phe Val Ala Lys Asp Tyr Glu Glu Leu Asp Leu
Ile Gly 115 120 125Ser Glu Glu Asp Met Ala Asn Leu Ile Phe Tyr Glu
Asn Pro Glu Val 130 135 140Ile Glu Glu Gly Phe Lys Pro Ile Ala Lys
Glu Lys Ser Ile Ser Asn145 150 155 160Gly Val Ile Asp Ile Leu Gly
Lys Asp Lys Asn Gly Asn Ile Met Val 165 170 175Leu Glu Leu Lys Arg
Val Arg Gly Ser Leu Gly Ala Val Ser Gln Leu 180 185 190Lys Arg Tyr
Val Asp Asn Leu Lys Glu Glu Asn Glu Gly Leu Arg Gly 195 200 205Met
Leu Val Ala Pro Ser Ile Thr Asp Ser Ala Met Lys Leu Leu Lys 210 215
220Glu Tyr Gly Leu Glu Phe Lys Glu Leu His Pro Pro Lys Lys Leu
Lys225 230 235 240Lys Glu Asp Ile Ile Lys Leu Asp Phe Phe 245
25041249PRTMethanosphaera 41Met Lys Tyr Lys Thr Ile Glu Asn Pro Ser
Thr Gln Glu Ala Tyr Glu1 5 10 15Leu Ile Lys Asp Gly Phe Asn Lys Lys
Ser Met Ile Ile Ile Leu Ala 20 25 30Gln Cys His Val Glu Tyr Glu Gly
Arg Ala Arg Ser Arg Leu Asp Lys 35 40 45Gly Asp Arg Leu Ile Leu Ile
Lys Lys Asp Gly Thr Phe Thr Ile His 50 55 60Gln Glu Leu Asn Leu Asp
Pro Val Asn Trp Gln Ala Pro Gly Cys Lys65 70 75 80Asn Lys Val Ser
Leu Lys Glu Asn Gln Ile Ile Leu Gln Ser Ile Lys 85 90 95Thr Lys Pro
Asp Glu Glu Ile Thr Val Tyr Leu Asp Thr Val Tyr Cys 100 105 110Ala
Thr Tyr Tyr Asn Cys Val Asp Thr Lys Asn Leu Glu Ile Arg Gly 115 120
125Tyr Glu Lys His Met Val Asp Leu Ala Trp Glu Lys Pro Glu Leu Ile
130 135 140Glu Lys Gly Phe Arg Pro Thr Arg Arg Glu Tyr Gln Thr Glu
Asn Gly145 150 155 160Phe Ile Asp Leu Met Gly Thr Asp Lys Asp Glu
Lys Leu Met Ile Leu 165 170 175Glu Phe Lys Ser Arg Lys Ala Gly Thr
Asn Ala Val Lys Gln Leu Lys 180 185 190Gly Tyr Val Glu Cys Phe Met
Asp Asn Lys Glu Phe Val Arg Gly Ile 195 200 205Ile Val Ala Pro Asp
Ile Thr Asp Asn Ala Leu Glu Leu Leu Lys Ser 210 215 220Leu Gln Met
Glu Phe Ile Pro Leu Asn Pro Pro Lys Asp Leu Leu Thr225 230 235
240Lys Lys Ala Ser Thr Leu Asp Ser Phe 24542249PRTMethanobacterium
lacus 42Met Lys Phe Leu Ser Glu Glu Asn Pro Asp His Gln Arg Thr Phe
Glu1 5 10 15Ile Ile Asn Glu Gly Leu Ser Lys Lys Ala Val Val Val Val
Val Ala 20 25 30Cys Cys Thr Val Asn Tyr Asp Gly Arg Ala Arg Ser Lys
Leu Gly Ala 35 40 45Gly Asp Arg Met Val Met Ile Lys Ser Asp Gly Ser
Phe Met Val His 50 55 60Gln Asn Ile Asn Leu Glu Pro Val Asn Trp Gln
Pro Pro Lys Ser Asn65 70 75 80Cys Ser Ala Val Leu Lys Asp Gly Asn
Ile Ile Leu Glu Gly Ser Arg 85 90 95Arg Ser Pro Pro Glu Arg Leu Gln
Val Val Ile His Lys Thr Tyr Val 100 105 110Ala Ser Tyr Tyr Asn Gly
Lys Asp Asn Lys Asp Leu Glu Leu Thr Gly 115 120 125Tyr Glu Glu Asn
Met Arg Glu Met Ile Phe Lys Thr Pro Asp Ile Ile 130 135 140Glu Glu
Gly Phe Arg Pro Ser Thr Lys Glu Tyr Pro Thr Ser Thr Gly145 150 155
160Phe Ile Asp Val Leu Gly Lys Asp Lys Asn Gly Asn Val Val Val Leu
165 170 175Glu Leu Lys Ser Arg Arg Ala Gly Ile Asn Ala Val Lys Gln
Leu Lys 180 185 190Arg Tyr Leu Asp Asp Phe Ser Asp His Lys Glu Phe
Val Arg Gly Ile 195 200 205Leu Val Ala Pro Ser Val Thr Asp Asp Ala
Ala Glu Leu Leu Glu Gly 210 215 220Phe Lys Leu Glu Phe Lys Pro Leu
Asp Pro Pro Arg Glu Phe Gly Ala225 230 235 240Asp Lys Asn Leu Thr
Leu Asp Phe Phe 24543249PRTMethanobacterium 43Met Leu Ile Glu Ile
Lys His Asn Pro Ser Asn Leu Glu Ser Met Glu1 5 10 15Ile Ile Asn Glu
Ala Leu Ser Lys Arg Ala Phe Leu Ile Leu Val Leu 20 25 30Cys Cys Lys
Val Asn Tyr Glu Gly Arg Ala Arg Ser Lys Leu Gly Leu 35 40 45Gly Glu
Arg Thr Val Leu Ile Lys Gly Asp Gly Ser Phe Ile Ile His 50 55 60Gln
Asp Arg Asn Leu Glu Pro Ile Asn Trp Gln Pro Pro Lys Thr Lys65 70 75
80Leu Ser Val Lys Leu Glu Asn Asp Val Ile Lys Ile Val Gly Met Arg
85 90 95Arg Lys Pro Lys Glu Gln Leu Glu Leu Ile Ile Ser Thr Val His
Leu 100 105 110Ile Ser Tyr Tyr Phe Gly Ser Asp Thr Lys Asp Ile Glu
Leu Ala Gly 115 120 125Tyr Glu Glu Asp Met Arg His Met Ile Met Asn
Asn Pro Asp Leu Ile 130 135 140Glu Lys Gly Phe Arg Pro Thr Ser Lys
Glu Tyr Gln Thr Pro Gln Gly145 150 155 160Phe Ile Asp Ile Tyr Gly
Lys Asp Ala Asn Gly Lys Ile Val Ile Ile 165 170 175Glu Leu Lys Ser
Arg Lys Ala Gly Ile Asn Ala Val Lys Gln Leu Lys 180 185 190Arg Tyr
Ile Asn Cys Phe Leu Asp Asn Lys Glu Phe Val Arg Gly Ile 195 200
205Leu Val Ala Pro Ser Ile Thr Asp Asp Ala Arg Glu Leu Leu Glu Asn
210 215 220Asn Lys Met Glu Tyr Ile Ser Leu Asp Pro Pro Lys Glu Leu
Lys Thr225 230 235 240Lys Thr Thr Thr Thr Leu Asp Tyr Phe
24544249PRTMethanobacterium 44Met Leu Leu Lys Ile Glu His Asn Pro
Thr Asn Leu Glu Ala Thr Glu1 5 10 15Leu Ile Asn Glu Ala Ile Ser Lys
Arg Ala Phe Met Ile Leu Val Val 20 25 30Cys Cys Lys Val Asn Tyr Glu
Gly Arg Ala Thr Ser Lys Leu Gly Phe 35 40 45Gly Glu Arg Thr Val Leu
Ile Lys Gly Asp Gly Ser Phe Ile Ile His 50 55 60Gln Asp Arg Asn Leu
Glu Pro Ile Asn Trp Gln Pro Pro Arg Thr Lys65 70 75 80Ile Gly Val
Lys Leu Glu Asp Asp Val Ile Lys Ile Met Gly Lys Arg 85 90 95Arg Lys
Pro Lys Glu Gln Leu Glu Leu Val Ile Arg Asn Val His Leu 100 105
110Ile Ser Tyr His Leu Gly Ser Asp Thr Lys Asp Ile Glu Leu Ala Gly
115 120 125Tyr Glu Glu Asp Met Arg Gln Met Ile Met Asp Asn Pro Glu
Leu Ile 130 135 140Glu Lys Gly Phe Arg Pro Thr Ser Lys Glu Tyr Gln
Thr Pro Gln Gly145 150 155 160Phe Ile Asp Ile Leu Gly Lys Asp Asp
Ala Gly Lys Leu Val Val Leu 165 170 175Glu Leu Lys Ser Arg Lys Ala
Gly Val Asn Ala Val Lys Gln Leu Leu 180 185 190Arg Tyr Val Asp Cys
Phe Ser Asp Asn Lys Glu Phe Val Arg Gly Ile 195 200 205Leu Val Ser
Pro Ser Ile Thr Glu Glu Ala Lys Glu Ile Leu Asn Glu 210 215 220Tyr
Gln Met Glu His Ile Ser Leu Ser Pro Pro Lys Glu Leu Lys Val225 230
235 240Lys Ser Ser Thr Thr Leu Asp Tyr Phe 24545251PRTPalaeococcus
pacificus 45Met Lys Val Lys Ser Lys Glu Asn Pro Ser Val Glu Glu Val
Val Glu1 5 10 15Ile Leu Ser Glu Gly Leu Ser Asn Glu Ala Ile Ile Thr
Leu Phe Ala 20 25 30His Cys Ser Val Phe Tyr Asp Gly Arg Ala Lys Ser
Glu Leu Gly Ala 35 40 45Gly Asp Arg Val Ile Met Ile Lys Pro Asp Gly
Thr Phe Leu Ile His 50 55 60Gln Lys Glu Lys Arg Val Pro Val Asn Trp
Gln Pro Pro Gly Ser Ile65 70 75 80Val Ser Phe Gln Ile Glu Glu Gly
Lys Ile Lys Leu Arg Ser Val Arg 85 90 95Arg Lys Pro Lys Glu Ile Leu
Glu Val Glu Leu Leu Lys Val Tyr Leu 100 105 110Ile Ser Tyr Phe Gln
Ala Glu Asp Tyr Glu Ala Leu Asn Leu Met Gly 115 120 125Ser Glu Ala
Glu Met Ala Asp Leu Ile Leu Gln Asn Pro Ser Ile Ile 130 135 140Glu
Glu Gly Phe Lys Ala Leu Gln Lys Glu Lys Pro Ile Lys His Gly145 150
155 160Ile Ile Asp Ile Tyr Gly Val Asp Arg Asp Gly Asn Ile Val Val
Leu 165 170 175Glu Leu Lys Arg Arg Arg Ala Asp Leu His Ala Val Ser
Gln Leu Lys 180 185 190Arg Tyr Val Asp Ala Leu Lys Glu Glu His Gly
Ser Val Arg Gly Ile 195 200 205Leu Val Ala Pro Ser Leu Thr Ser Gly
Ala Glu Lys Leu Leu Lys Asp 210 215 220Leu Gly Leu Glu Phe Lys Lys
Leu Asn Pro Pro Lys Arg Glu Lys Ala225 230 235 240Arg Lys Gly Lys
Gln Lys Thr Leu Asp Met Leu 245 25046242PRTMethanobacterium
formicicum 46Asn Pro Asp Thr Pro Arg Val Leu Glu Ile Ile Asn Glu
Gly Leu Ser1 5 10 15Lys Arg Ala Val Ile Thr Ile Met Ala Cys Cys Arg
Val Asp Tyr Asp 20 25 30Gly Arg Ala Val Ser Arg Leu Gly Leu Gly Asp
Arg Ile Ile Leu Ile 35 40 45Lys Ser Asp Gly Ser Phe Ile Ile His Gln
Asp Arg Asn Leu Asp Pro 50 55 60Val Asn Trp Gln Pro Pro Lys Thr Lys
Val Thr Ala Asp Ile Tyr Gln65 70 75 80Gly Met Val Lys Ile Lys Gly
Val Arg Arg Asn Pro Ser Glu Ser Leu 85 90 95Glu Val Lys Ile Leu Gln
Thr His Met Ile Ser Tyr Phe Ile Gly Glu 100 105 110Asp Ser Glu Ser
Leu Glu Leu Ala Gly Tyr Glu Ala Asn Met Gly Asp 115 120 125Leu Ile
Phe Lys Asp Pro Glu Val Phe Glu Lys Gly Phe Arg Pro Thr 130 135
140Ser Arg Glu Tyr His Thr Pro Gln Gly Phe Ile Asp Ile Leu Gly
Lys145 150 155 160Asp Gln Asp Gly Asn Ile Thr Ile Leu Glu Leu Lys
Ser Arg Lys Ala 165 170 175Gly Thr Asn Ala Val Lys Gln Leu Arg Arg
Tyr Val Asp Cys Phe Ser 180 185 190Asp His Lys Glu Lys Val Arg Gly
Val Leu Val Ala Pro Ser Ala Thr 195 200 205Asp Asp Ala Leu Glu Leu
Leu Glu Glu Gln Gly Met Glu Phe Lys Ala 210 215 220Leu Glu Pro Pro
Arg Glu Leu Gly Thr Asp Lys Val Val Thr Leu Glu225 230 235 240Asn
Phe47247PRTPyrococcus kukulkanii 47Lys Ile Val Val Arg Glu Asn Pro
Thr Val Glu Asp Val Lys Glu Leu1 5 10 15Leu Glu Phe Ala Glu Lys His
Asn Gly Met Val Thr Ile Phe Ala Arg 20 25 30Cys Arg Val Tyr Tyr Glu
Gly Arg Ala Lys Ser Glu Leu Gly Glu Gly 35 40 45Asp Arg Ile Ile Ile
Ile Lys Pro Asp Gly Ser Phe Leu Ile His Gln 50 55 60Asn Lys Lys Arg
Glu Pro Val Asn Trp Gln Pro Pro Gly Ser Val Val65 70 75 80Lys Ile
Glu Gly Asn Lys Val Val Ser Ile Arg Arg Lys Pro Arg Glu 85 90 95Lys
Leu Glu Val Glu Leu Ile Glu Ser Tyr Ala Ile Thr Val Phe Leu 100 105
110Ala Glu Asp Tyr Glu Glu Leu Ser Leu Thr Gly Ser Glu Ala Glu Met
115 120 125Ala Lys Leu Ile Phe Glu Lys Pro Glu Val Ile Glu Glu Gly
Phe Lys 130 135 140Pro Met Phe Lys Glu Lys Pro Ile Lys His Gly Ile
Val Asp Ile Leu145 150 155 160Gly Val Asp Arg Glu Gly Asn Val Val
Val Leu Glu Leu Lys Arg Arg 165 170 175Arg Ala Asp Leu His Ala Val
Ser Gln Leu Lys Arg Tyr Val Asp Ser 180 185 190Leu Lys Glu Glu Tyr
Gly Asp Lys Val Arg Gly Ile Leu Val Ala Pro 195 200 205Ser Leu Thr
Glu Gly Ala Arg Lys Leu Leu Glu Lys Glu Gly Leu Glu 210 215 220Phe
Arg Lys Leu Glu Pro Pro Lys Arg Glu Ser Arg Lys Lys Ser Lys225 230
235 240Gln Lys Thr Leu Asp Phe Phe 24548247PRTThermococcus
guaymasensis 48Lys Val Glu Leu Arg Glu Asn Pro Ser Pro Glu Glu Ile
Lys Leu Leu1 5 10 15Val Asp Ser Ala Val Ser Ser Glu Gly Ile Leu Thr
Ile Phe Ala Arg 20 25 30Cys Arg Val His Tyr Asp Gly Arg Ala Lys Ser
Glu Leu Gly Pro Gly 35 40 45Asp Arg Val Ile Ile Val Lys Pro Asp Gly
Ser Phe Leu Ile His Gln 50 55 60Lys Glu Lys Arg Glu Pro Val Asn Trp
Gln Pro Pro Gly Ser Val Val65 70 75 80Arg Leu Glu Leu Arg Glu Lys
Pro Val Leu Val Ser Val Arg Arg Lys 85 90 95Pro Arg Glu Thr Leu Glu
Val Glu Leu Glu Glu Val Tyr Leu Ile Thr 100 105 110Val Phe His Ala
Glu Asp Tyr Glu Glu Leu Ala Leu Thr Gly Ser Glu 115 120 125Ala Glu
Met Ala Glu Leu Ile Phe Glu Asn Pro Glu Val Ile Glu Phe 130 135
140Gly Phe Lys Pro Leu Tyr Arg Glu Lys Pro Ile Lys His Gly Ile
Val145 150 155 160Asp Val Leu Gly Val Asp Arg Glu Gly Asn Ile Val
Val Leu Glu Leu 165 170 175Lys Arg Arg Arg Ala Asp Leu His Ala Val
Ser Gln Leu Lys Arg Tyr 180 185 190Val Glu Thr Leu Arg Glu Glu His
Glu Asn Val Arg Gly Ile Leu Val 195 200 205Ala Pro Ser Leu Thr Ser
Gly Ala Lys Lys Leu Leu Glu Lys Glu Gly 210 215 220Leu Glu Phe Arg
Lys Leu Glu Pro Pro Lys Arg Asp Arg Lys Ser Arg225 230 235 240Gly
Lys Gln Leu Lys Leu Phe 24549247PRTThermococcus eurythermalis 49Lys
Val Glu Leu Arg Glu Asn Pro
Ser Pro Glu Glu Ile Lys Leu Leu1 5 10 15Val Asp Ser Ala Val Ser Ser
Glu Gly Val Leu Thr Ile Phe Ala Arg 20 25 30Cys Arg Val His Tyr Asp
Gly Arg Ala Lys Ser Glu Leu Gly Pro Gly 35 40 45Asp Arg Val Ile Ile
Val Lys Pro Asp Gly Ser Phe Leu Ile His Gln 50 55 60Lys Glu Lys Arg
Glu Pro Val Asn Trp Gln Pro Pro Gly Ser Val Val65 70 75 80Arg Leu
Glu Leu Arg Glu Lys Pro Val Leu Val Ser Val Arg Arg Lys 85 90 95Pro
Arg Glu Thr Leu Glu Val Glu Leu Glu Glu Val Tyr Leu Ile Thr 100 105
110Val Phe His Ala Glu Asp Tyr Glu Glu Leu Ala Leu Thr Gly Ser Glu
115 120 125Ala Glu Met Ala Glu Leu Ile Phe Glu Asn Pro Glu Val Ile
Glu Pro 130 135 140Gly Phe Lys Pro Leu Tyr Arg Glu Lys Pro Ile Lys
His Gly Ile Val145 150 155 160Asp Val Leu Gly Val Asp Arg Asp Gly
Asn Leu Val Val Leu Glu Leu 165 170 175Lys Arg Arg Arg Ala Asp Leu
His Ala Val Ser Gln Leu Lys Arg Tyr 180 185 190Val Glu Thr Leu Arg
Glu Glu His Glu Asn Val Arg Gly Ile Leu Val 195 200 205Ala Pro Ser
Leu Thr Ser Gly Ala Lys Lys Leu Leu Glu Lys Glu Gly 210 215 220Leu
Glu Phe Arg Lys Leu Glu Pro Pro Lys Arg Asp Arg Lys Ser Arg225 230
235 240Gly Lys Gln Leu Lys Leu Phe 24550247PRTThermococcus nautili
50Lys Val Glu Leu Arg Glu Asn Pro Ser Pro Glu Glu Ile Lys Leu Leu1
5 10 15Val Asp Ser Ala Val Ser Ser Glu Gly Val Leu Thr Ile Phe Ala
Arg 20 25 30Cys Arg Val His Tyr Asp Gly Arg Ala Lys Ser Glu Leu Gly
Pro Gly 35 40 45Asp Arg Val Ile Ile Val Lys Pro Asp Gly Ser Phe Leu
Ile His Gln 50 55 60Lys Glu Lys Arg Glu Pro Val Asn Trp Gln Pro Pro
Gly Ser Val Val65 70 75 80Arg Leu Glu Leu Arg Glu Lys Pro Val Leu
Val Ser Val Arg Arg Lys 85 90 95Pro Arg Glu Thr Leu Glu Val Glu Leu
Glu Glu Val Tyr Leu Ile Thr 100 105 110Val Phe His Ala Glu Asp Tyr
Glu Glu Leu Ala Leu Thr Gly Ser Glu 115 120 125Ala Glu Met Ala Glu
Leu Ile Phe Glu Asn Pro Glu Val Ile Glu Pro 130 135 140Gly Phe Lys
Pro Leu Tyr Arg Glu Lys Pro Ile Lys His Gly Ile Val145 150 155
160Asp Val Leu Gly Val Asp Lys Asp Gly Asn Leu Val Val Leu Glu Leu
165 170 175Lys Arg Arg Arg Ala Asp Leu His Ala Val Ser Gln Leu Lys
Arg Tyr 180 185 190Val Glu Thr Leu Arg Glu Glu His Glu Asn Val Arg
Gly Ile Leu Val 195 200 205Ala Pro Ser Leu Thr Ser Gly Ala Lys Lys
Leu Leu Glu Lys Glu Gly 210 215 220Leu Glu Phe Arg Lys Leu Glu Pro
Pro Lys Arg Gly Arg Lys Ser Arg225 230 235 240Gly Lys Gln Leu Lys
Leu Phe 24551247PRTThermococcus gammatolerans 51Lys Val Glu Leu Arg
Glu Asn Pro Ser Pro Glu Glu Ile Lys Leu Leu1 5 10 15Val Asp Leu Ala
Ile Ser Ser Glu Gly Val Leu Thr Ile Phe Ala Arg 20 25 30Cys Arg Val
His Tyr Asp Gly Arg Ala Lys Ser Glu Leu Gly Pro Gly 35 40 45Asp Arg
Val Ile Ile Val Lys Pro Asp Gly Ser Phe Leu Ile His Gln 50 55 60Lys
Glu Lys Arg Glu Pro Val Asn Trp Gln Pro Pro Gly Ser Val Val65 70 75
80Arg Leu Glu Leu Arg Glu Lys Pro Val Leu Ile Ser Val Arg Arg Lys
85 90 95Pro Arg Glu Thr Leu Glu Val Glu Leu Asp Glu Val Tyr Leu Ile
Thr 100 105 110Val Phe His Ala Glu Asp Tyr Glu Glu Leu Ala Leu Thr
Gly Ser Glu 115 120 125Ala Glu Met Ala Glu Leu Ile Phe Glu Asn Pro
Glu Val Ile Glu Leu 130 135 140Gly Phe Lys Pro Leu Tyr Arg Glu Lys
Pro Ile Arg His Gly Ile Val145 150 155 160Asp Val Leu Gly Val Asp
Arg Asp Gly Asn Leu Val Val Leu Glu Leu 165 170 175Lys Arg Arg Arg
Ala Asp Leu His Ala Val Ser Gln Leu Lys Arg Tyr 180 185 190Val Glu
Thr Leu Arg Glu Glu His Glu Asn Val Arg Gly Ile Leu Val 195 200
205Ala Pro Ser Leu Thr Ser Gly Ala Lys Lys Leu Leu Glu Lys Glu Gly
210 215 220Leu Glu Phe Arg Lys Leu Glu Pro Pro Lys Arg Asp Gly Lys
Ser Arg225 230 235 240Gly Arg Gln Leu Arg Leu Phe
24552247PRTThermococcus 52Lys Val Glu Leu Arg Glu Asn Pro Ser Pro
Glu Glu Ile Lys Leu Leu1 5 10 15Val Asp Ser Ala Val Ser Ser Glu Gly
Val Leu Thr Ile Phe Ala Arg 20 25 30Cys Arg Val His Tyr Asp Gly Arg
Ala Lys Ser Glu Leu Gly Pro Gly 35 40 45Asp Arg Val Ile Ile Val Lys
Pro Asp Gly Ser Phe Leu Ile His Gln 50 55 60Lys Glu Lys Arg Glu Pro
Val Asn Trp Gln Pro Pro Gly Ser Val Val65 70 75 80Arg Leu Glu Leu
Arg Glu Lys Pro Val Leu Val Ser Val Arg Arg Lys 85 90 95Pro Arg Glu
Thr Leu Glu Val Glu Leu Glu Glu Val Tyr Leu Ile Thr 100 105 110Val
Phe His Ala Glu Asp Tyr Glu Glu Leu Ala Leu Thr Gly Ser Glu 115 120
125Ala Glu Met Ala Glu Leu Ile Phe Glu Asn Pro Glu Val Ile Glu Pro
130 135 140Gly Phe Lys Pro Leu Tyr Arg Glu Lys Pro Ile Lys His Gly
Ile Val145 150 155 160Asp Val Leu Gly Val Asp Lys Asp Gly Asn Leu
Val Val Leu Glu Leu 165 170 175Lys Arg Arg Arg Ala Asp Leu His Ala
Val Ser Gln Leu Lys Arg Tyr 180 185 190Val Glu Thr Leu Arg Glu Lys
His Glu Asn Val Arg Gly Ile Leu Val 195 200 205Ala Pro Ser Leu Thr
Ser Gly Ala Arg Lys Leu Leu Glu Lys Glu Gly 210 215 220Leu Glu Phe
Arg Lys Leu Glu Pro Pro Lys Arg Gly Arg Lys Ser Lys225 230 235
240Gly Arg Gln Leu Lys Leu Phe 24553252PRTMethanotorris igneus
53Lys Val Asn Tyr Leu Val Asn Pro Lys Ala Glu Asp Ile Val Glu Leu1
5 10 15Leu Ala Ser Gly Ile Val Asn Asp Ser Ile Leu Val Phe Phe Ala
Phe 20 25 30Cys Arg Val Arg Tyr Asp Gly Arg Ala Lys Ser Glu Leu Glu
Pro Gly 35 40 45Asp Arg Ile Ile Ile Ile Lys Pro Asp Gly Ser Phe Leu
Val His Lys 50 55 60Asn Thr Lys Arg Glu Pro Val Asn Trp Gln Pro Pro
Gly Ser Val Val65 70 75 80Ser Trp Glu Val His Asp Gly Lys Leu Val
Leu Lys Ser Val Arg Lys 85 90 95Lys Pro Arg Glu Ile Leu Gln Val Glu
Leu Ile Lys Val Tyr His Ala 100 105 110Cys Ser Phe Gln Cys Glu Asp
Tyr Glu Glu Leu Ser Leu Thr Gly Ser 115 120 125Glu Ala Glu Met Ala
Glu Leu Ile Phe Glu Asn Pro Ser Leu Ile Glu 130 135 140Asp Gly Phe
Lys Pro Leu Phe Lys Glu Lys Gln Ile Asn His Gly Ile145 150 155
160Ile Asp Ile Leu Gly Lys Asp Lys Ser Gly Arg Trp Val Val Ile Glu
165 170 175Leu Lys Arg Arg Arg Ala Asp Leu Gln Ala Val Ser Gln Leu
Lys Arg 180 185 190Tyr Val Glu Cys Leu Lys Tyr Glu Tyr Gly Glu Gly
Asn Ile Arg Gly 195 200 205Ile Leu Val Ala Pro Ser Leu Thr Ser Gly
Ala Lys Lys Leu Leu Glu 210 215 220Glu Glu Asn Leu Glu Phe Arg Glu
Leu Lys Pro Pro Lys Lys Glu Arg225 230 235 240Leu Lys Glu Asn Lys
Gln Thr Thr Leu Asp Phe Tyr 245 25054249PRTThermococcus barophilus
54Met Lys Val Glu Ala Lys Val Glu Pro Ser His Glu Glu Ile Ile Glu1
5 10 15Ile Leu Asp Lys Ala Leu Ser Val Glu Ala Ile Ile Thr Leu Phe
Ala 20 25 30Tyr Cys Arg Val Phe Tyr Glu Gly Arg Ala Lys Ser Glu Leu
Gly Pro 35 40 45Gly Asp Arg Val Ile Ile Ile Lys Pro Asp Gly Ser Phe
Leu Ile His 50 55 60Gln Lys Asn Lys Arg Glu Pro Val Asn Trp Gln Pro
Pro Gly Ser Val65 70 75 80Val Ser Ile Val Leu Glu Asp Gly Arg Ile
Met Leu Arg Ser Val Arg 85 90 95Arg Lys Pro Lys Glu Thr Leu Glu Val
Glu Leu Ile Lys Thr Tyr Leu 100 105 110Val Ser Tyr Phe Gln Ala Glu
Asp Tyr Glu Glu Leu Thr Leu Thr Gly 115 120 125Ser Glu Ala Glu Met
Ala Asp Leu Ile Phe Glu Asn Pro Ser Leu Ile 130 135 140Glu Glu Gly
Phe Lys Pro Leu Phe Lys Glu Lys Pro Ile Lys His Gly145 150 155
160Ile Val Asp Val Leu Gly Lys Asp Lys His Gly Asn Leu Val Val Leu
165 170 175Glu Leu Lys Arg Arg Arg Ala Asp Leu His Ala Val Ser Gln
Leu Lys 180 185 190Arg Tyr Val Asp Ser Leu Arg Glu Glu His Lys Asn
Val Arg Gly Ile 195 200 205Leu Val Ala Pro Ser Leu Thr Ala Gly Ala
Lys Lys Leu Leu Glu Lys 210 215 220Glu Gly Leu Glu Phe Lys Lys Leu
Asn Pro Pro Lys Arg Glu Lys Arg225 230 235 240Lys Lys Gly Lys Gln
Lys Thr Leu Asp 24555249PRTThermococcus barophilus 55Met Lys Val
Glu Ala Lys Val Gly Pro Ser His Glu Glu Ile Val Glu1 5 10 15Ile Leu
Asn Lys Ala Leu Ser Val Glu Ala Ile Ile Thr Leu Phe Ala 20 25 30Tyr
Cys Arg Val Phe Tyr Glu Gly Arg Ala Lys Ser Glu Leu Gly Pro 35 40
45Gly Asp Arg Val Ile Ile Ile Lys Pro Asp Gly Ser Phe Leu Ile His
50 55 60Gln Lys Asn Lys Arg Glu Pro Val Asn Trp Gln Pro Pro Gly Ser
Val65 70 75 80Val Ser Ile Val Leu Gly Asp Gly Arg Ile Met Leu Arg
Ser Val Arg 85 90 95Arg Lys Pro Lys Glu Thr Leu Glu Val Glu Leu Ile
Lys Thr Tyr Leu 100 105 110Val Ser Tyr Phe Gln Ala Glu Asp Tyr Glu
Glu Leu Thr Leu Thr Gly 115 120 125Ser Glu Ala Glu Met Ala Asp Leu
Ile Phe Glu Asn Pro Ser Leu Ile 130 135 140Glu Glu Gly Phe Lys Pro
Leu Phe Lys Glu Lys Pro Ile Lys His Gly145 150 155 160Ile Val Asp
Val Leu Gly Lys Asp Lys His Gly Asn Leu Val Val Leu 165 170 175Glu
Leu Lys Arg Arg Arg Ala Asp Leu His Ala Val Ser Gln Leu Lys 180 185
190Arg Tyr Val Asp Ser Leu Arg Glu Glu His Lys Asn Val Arg Gly Ile
195 200 205Leu Val Ala Pro Ser Leu Thr Ala Gly Ala Lys Lys Leu Leu
Glu Lys 210 215 220Glu Gly Leu Glu Phe Lys Lys Leu Asn Pro Pro Lys
Arg Glu Lys Arg225 230 235 240Lys Lys Gly Lys Gln Lys Thr Leu Asp
24556242PRTMethanobacterium 56Asn Pro Asp Thr Gln Arg Val Leu Asp
Ile Ile Asn Glu Gly Leu Ser1 5 10 15Lys Arg Ala Val Ile Thr Ile Met
Ala Ser Cys Arg Val Tyr Tyr Asp 20 25 30Gly Arg Ala Val Ser Arg Leu
Glu Leu Gly Asp Arg Ile Ile Met Ile 35 40 45Lys Ser Asp Gly Ser Phe
Ile Ile His Gln Asp Arg Asn Leu Glu Pro 50 55 60Val Asn Trp Gln Pro
Pro Lys Thr Lys Val Thr Val Asp Thr His Gln65 70 75 80Gly Met Val
Lys Ile Arg Gly Val Arg Arg Ser Pro Ser Glu Ser Leu 85 90 95Glu Val
Glu Ile Leu Gln Thr His Leu Val Ser Tyr Phe Ile Gly Glu 100 105
110Asp Val Glu Ser Leu Glu Leu Ala Gly Tyr Glu Ala Asn Met Gly Asp
115 120 125Leu Ile Phe Lys Asp Pro Glu Val Ile Glu Lys Gly Phe Arg
Pro Thr 130 135 140Ser Arg Glu Tyr His Thr Pro Gln Gly Phe Ile Asp
Val Leu Gly Lys145 150 155 160Asp Gln Asn Gly Asn Ile Thr Ile Leu
Glu Leu Lys Ser Arg Lys Ala 165 170 175Gly Val Asn Ala Val Lys Gln
Leu Arg Arg Tyr Val Asp Cys Phe Ser 180 185 190Asp His Lys Asp Thr
Val Arg Gly Val Leu Val Ala Pro Ser Ile Thr 195 200 205Asp Asp Ala
Arg Glu Leu Leu Glu Glu Gln Lys Met Glu Phe Lys Glu 210 215 220Leu
Glu Pro Pro Arg Glu Leu Gly Thr Asp Lys Val Val Thr Leu Glu225 230
235 240Lys Phe57249PRTPyrococcus yayanosii 57Lys Ala Glu Val Arg
Met Glu Pro Thr Pro Lys Glu Leu Ala Glu Leu1 5 10 15Phe Asp Leu Ala
Arg Lys Leu Glu Gly Met Leu Ile Ile Phe Ala Arg 20 25 30Cys Arg Val
His Tyr Glu Gly Arg Ala Lys Ser Glu Leu Gly Glu Gly 35 40 45Asp Arg
Ile Ile Met Ile Lys Pro Asp Gly Ser Phe Leu Ile His Gln 50 55 60Lys
Lys Lys Arg Glu Pro Val Asn Trp Gln Pro Pro Gly Ser Lys Val65 70 75
80Arg Leu Glu Leu Arg Asn Val Pro Thr Ile Val Ser Val Arg Arg Lys
85 90 95Pro Arg Glu Ile Leu Glu Val Glu Leu Leu Glu Thr Tyr Met Val
Ser 100 105 110Ala Phe Phe Ala Glu Asp Tyr Glu Ala Leu Lys Leu Ser
Gly Ser Glu 115 120 125Ala Glu Met Ala Ala Leu Ile Phe Ser Asn Pro
Asp Val Ile Glu Pro 130 135 140Gly Phe Lys Pro Leu Phe Arg Glu Lys
Pro Val Arg His Gly Ile Val145 150 155 160Asp Val Leu Gly Val Asp
Lys Glu Gly Asn Leu Val Val Leu Glu Leu 165 170 175Lys Arg Arg Lys
Ala Asp Leu His Ala Val Ser Gln Leu Lys Arg Tyr 180 185 190Val Glu
Ala Leu Arg Glu Glu Asn Pro Gly Lys Ile Val Arg Gly Ile 195 200
205Leu Val Ala Pro Ser Ile Thr Ala Gly Ala Gln Arg Leu Leu Glu Lys
210 215 220Glu Gly Leu Glu Phe Arg Arg Met Glu Pro Pro Lys Arg Lys
Glu Lys225 230 235 240Arg Lys Ser Arg Gln Lys Thr Leu Asp
24558248PRTThermococcus litoralis 58Met Lys Val Glu Ala Lys Leu Asn
Pro Thr Tyr Glu Glu Ile Val Asp1 5 10 15Ile Phe Asn Arg Ala Leu Ser
Lys Glu Ala Ile Val Asn Ile Phe Ala 20 25 30His Cys Arg Val Phe Tyr
Asp Gly Arg Ala Lys Ser Glu Leu Gly Pro 35 40 45Gly Asp Arg Val Ile
Leu Ile Lys Pro Asp Gly Ser Phe Leu Ile His 50 55 60Gln Lys Glu Lys
Arg Glu Pro Val Asn Trp Gln Pro Pro Gly Ser Ser65 70 75 80Val Gly
Leu Glu Val Lys Glu Asp Lys Ile Phe Leu Arg Ser Ile Arg 85 90 95Arg
Lys Pro Arg Glu Ile Leu Glu Val Glu Leu Leu Asn Val Tyr Leu 100 105
110Ile Ser Tyr Phe Gln Ala Glu Asp Tyr Glu Glu Leu Ala Leu Thr Gly
115 120 125Ser Glu Ala Glu Met Ala Asp Leu Ile Phe Glu Asn Pro Ser
Leu Ile 130 135 140Glu Asp Gly Phe Lys Pro Leu Phe Lys Glu Lys Pro
Ile Lys His Gly145 150 155 160Ile Val Asp Val Leu Gly Val Asp Lys
Glu Gly Asn Ile Val Ile Leu 165 170 175Glu Leu Lys Arg Arg Arg Ala
Asp Leu His Ala Val Ser Gln Leu Lys 180 185 190Arg Tyr Val Glu Ala
Met Arg Glu Glu His Glu Lys Val Arg Gly Ile 195 200 205Leu Val Ala
Pro Ser Leu Thr Ser Gly Ala Lys Lys Leu Leu Glu Lys 210 215 220Glu
Gly Leu Glu Phe Arg Lys Leu Thr Pro Pro Lys Arg Gly Lys
Ser225 230 235 240Lys Arg Gly Arg Gln Lys Thr Leu
24559248PRTThermococcus 59Met Lys Val Glu Ala Lys Leu Asn Pro Thr
Tyr Glu Glu Ile Val Asp1 5 10 15Ile Phe Asn Arg Ala Leu Ser Lys Glu
Ala Ile Val Asn Ile Phe Ala 20 25 30His Cys Arg Val Phe Tyr Asp Gly
Arg Ala Lys Ser Glu Leu Gly Pro 35 40 45Gly Asp Arg Val Ile Leu Ile
Lys Pro Asp Gly Ser Phe Leu Ile His 50 55 60Gln Lys Glu Lys Arg Glu
Pro Val Asn Trp Gln Pro Pro Gly Ser Ser65 70 75 80Val Gly Leu Glu
Val Lys Glu Gly Arg Ile Phe Leu Arg Ser Ile Arg 85 90 95Arg Lys Pro
Arg Glu Ile Leu Glu Val Glu Leu Leu His Val Tyr Leu 100 105 110Ile
Ser Tyr Phe Gln Ala Glu Asp Tyr Glu Glu Leu Ala Leu Thr Gly 115 120
125Ser Glu Ala Glu Met Ala Asp Leu Ile Phe Glu Asn Pro Ser Val Ile
130 135 140Glu Asp Gly Phe Lys Pro Leu Phe Lys Glu Lys Pro Ile Lys
His Gly145 150 155 160Ile Val Asp Val Leu Gly Val Asp Lys Glu Gly
Asn Ile Val Val Leu 165 170 175Glu Leu Lys Arg Arg Arg Ala Asp Leu
His Ala Val Ser Gln Leu Lys 180 185 190Arg Tyr Val Glu Ala Met Lys
Glu Glu His Glu Lys Val Arg Gly Ile 195 200 205Leu Val Ala Pro Ser
Leu Thr Ser Gly Ala Lys Lys Leu Leu Glu Lys 210 215 220Glu Gly Leu
Glu Phe Arg Arg Leu Thr Pro Pro Lys Arg Gly Lys Ser225 230 235
240Lys Arg Gly Arg Gln Lys Thr Leu 24560243PRTPyrococcus horikoshii
60Glu Asn Pro Thr Val Glu Glu Val Lys Glu Leu Leu Asp Ile Ala Glu1
5 10 15Lys His Gly Gly Val Val Thr Ile Phe Ala Arg Cys Arg Val Tyr
Tyr 20 25 30Glu Gly Arg Ala Lys Ser Glu Leu Gly Glu Gly Asp Arg Ile
Val Ile 35 40 45Ile Lys Pro Asp Gly Ser Phe Leu Ile His Gln Asn Lys
Lys Arg Glu 50 55 60Pro Val Asn Trp Gln Pro Pro Gly Ser Lys Val Ser
Met Arg Glu Asn65 70 75 80Ser Ile Ile Ser Ile Arg Arg Lys Pro His
Glu Arg Leu Glu Val Glu 85 90 95Leu Met Glu Val Tyr Ala Val Thr Val
Phe Leu Ala Glu Asp Tyr Glu 100 105 110Glu Leu Ala Leu Thr Gly Ser
Glu Ala Glu Met Ala Lys Leu Ile Phe 115 120 125Glu Asn Pro Asn Val
Ile Glu Glu Gly Phe Lys Pro Met Phe Arg Glu 130 135 140Lys Gln Ile
Lys His Gly Ile Val Asp Ile Met Gly Leu Asp Lys Asp145 150 155
160Gly Asn Ile Val Val Leu Glu Leu Lys Arg Arg Lys Ala Asp Leu His
165 170 175Ala Val Ser Gln Leu Lys Arg Tyr Val Asp Ser Leu Lys Glu
Glu Tyr 180 185 190Gly Glu Lys Val Arg Gly Ile Leu Val Ala Pro Ser
Leu Thr Glu Gly 195 200 205Ala Lys Lys Leu Leu Glu Lys Glu Gly Leu
Glu Phe Arg Lys Leu Glu 210 215 220Pro Pro Lys Asn Asn Asp Asn Lys
Arg Glu Val Lys Gln Lys Thr Leu225 230 235 240Asp Phe
Phe61247PRTThermococcus thioreducens 61Lys Val Glu Ala Val Gln Asn
Pro Ser Arg Asp Glu Leu Met Arg Met1 5 10 15Val Asp Ser Ala Leu Ser
Ala Glu Ala Met Leu Thr Ile Phe Ala Arg 20 25 30Cys Lys Val His Tyr
Asp Gly Arg Ala Lys Ser Glu Leu Gly Ser Gly 35 40 45Asp Arg Val Ile
Ile Ile Lys Pro Asp Gly Ala Phe Leu Ile His Gln 50 55 60Ser Arg Lys
Arg Glu Pro Val Asn Trp Gln Pro Pro Gly Ser Phe Val65 70 75 80Met
Met Glu Glu Arg Asp Gly Ile Leu Val Leu Arg Ser Val Arg Arg 85 90
95Lys Pro Lys Glu Ile Leu Glu Val Glu Leu Glu Glu Val Tyr Leu Ile
100 105 110Ser Leu Phe Lys Ala Glu Asp Tyr Glu Glu Leu Ala Leu Thr
Gly Ser 115 120 125Glu Ala Glu Met Ala Glu Met Val Phe Arg Asn Pro
Glu Leu Ile Glu 130 135 140Pro Gly Phe Lys Pro Leu Phe Arg Glu Lys
Gln Ile Gly His Gly Ile145 150 155 160Val Asp Ile Leu Gly Arg Asp
Arg Asp Gly Asn Leu Val Val Leu Glu 165 170 175Leu Lys Arg Arg Lys
Ala Asp Leu His Ala Val Ser Gln Leu Lys Arg 180 185 190Tyr Val Glu
Ala Leu Lys Arg Glu His Glu Thr Val Arg Gly Ile Leu 195 200 205Val
Ala Pro Ser Leu Thr Ala Gly Ala Lys Lys Leu Leu Glu Lys Glu 210 215
220Gly Leu Glu Phe Arg Arg Val Gln Pro Pro Lys Arg Glu Lys Phe
Gly225 230 235 240Arg Gly Arg Gln Lys Thr Leu
24562244PRTThermococcus chitonophagus 62Val Arg Glu Lys Pro Thr Val
Glu Asp Val Lys Glu Leu Leu Glu Phe1 5 10 15Ala Glu Lys His Asn Gly
Met Val Thr Ile Phe Ala Arg Cys Arg Val 20 25 30Tyr Tyr Glu Gly Arg
Ala Lys Ser Glu Leu Gly Glu Gly Asp Arg Ile 35 40 45Ile Ile Ile Lys
Pro Asp Gly Ser Phe Leu Ile His Gln Asn Lys Lys 50 55 60Arg Glu Pro
Val Asn Trp Gln Pro Pro Gly Ser Val Val Arg Val Glu65 70 75 80Gly
Asn Lys Val Ile Ser Ile Arg Arg Lys Pro Arg Glu Lys Leu Glu 85 90
95Val Glu Leu Ile Glu Ser Tyr Ala Ile Thr Val Phe Leu Ala Glu Asp
100 105 110Tyr Glu Glu Leu Ser Leu Thr Gly Ser Glu Ala Glu Met Ala
Lys Leu 115 120 125Ile Phe Glu Lys Pro Glu Val Ile Glu Glu Gly Phe
Lys Pro Met Phe 130 135 140Lys Glu Lys Pro Ile Lys His Gly Ile Val
Asp Ile Leu Gly Ile Asp145 150 155 160Arg Glu Gly Asn Val Val Val
Leu Glu Leu Lys Arg Arg Arg Ala Asp 165 170 175Leu His Ala Val Ser
Gln Leu Lys Arg Tyr Val Asp Ala Leu Lys Glu 180 185 190Glu Tyr Gly
Glu Arg Val Arg Gly Ile Leu Val Ala Pro Ser Leu Thr 195 200 205Glu
Gly Ala Arg Lys Leu Leu Glu Lys Glu Gly Leu Glu Phe Lys Lys 210 215
220Leu Glu Pro Pro Lys Arg Glu Ser Arg Lys Lys Ser Lys Gln Arg
Thr225 230 235 240Leu Asp Phe Phe63245PRTPyrococcus 63Ile Lys Glu
Lys Pro Thr Ala Asp Glu Ile Lys Glu Leu Leu Asp Ile1 5 10 15Ala Glu
Lys Tyr Gly Gly Val Ile Thr Ile Phe Ala Lys Cys Lys Val 20 25 30Tyr
Tyr Glu Gly Arg Ala Lys Ser Glu Leu Gly Glu Gly Asp Arg Ile 35 40
45Ile Ile Ile Lys Pro Asp Gly Ser Phe Leu Ile His Gln Asn Lys Lys
50 55 60Arg Glu Pro Val Asn Trp Gln Pro Pro Gly Ser Ser Val Arg Ile
Glu65 70 75 80Gly Asn Thr Ile Ile Ser Ile Arg Arg Lys Pro Arg Glu
Lys Leu Glu 85 90 95Val Glu Val Leu Glu Ala Tyr Ser Gly Ile Val Phe
Phe Ala Glu Asp 100 105 110Tyr Glu Glu Leu Ala Leu Thr Gly Ser Glu
Ala Glu Met Ala Glu Leu 115 120 125Ile Phe Gln Asn Pro Asp Ile Ile
Glu Lys Gly Phe Lys Pro Leu Phe 130 135 140Arg Glu Lys Pro Ile Lys
His Gly Ile Val Asp Ile Leu Gly Val Asp145 150 155 160Lys Glu Gly
Asn Ile Val Val Leu Glu Leu Lys Arg Arg Arg Ala Asp 165 170 175Leu
His Ala Val Ser Gln Leu Lys Arg Tyr Val Glu Ser Leu Lys Glu 180 185
190Glu Tyr Lys Arg Lys Val Arg Gly Ile Leu Val Ala Pro Ser Leu Thr
195 200 205Glu Gly Ala Arg Lys Leu Leu Glu Lys Glu Gly Leu Glu Phe
Lys Arg 210 215 220Leu Glu Pro Pro Lys Arg Lys Asp Lys Lys Ser Arg
Gly Lys Gln Lys225 230 235 240Thr Leu Asp Phe Phe
24564245PRTPyrococcus 64Ile Lys Glu Lys Pro Thr Ala Asp Glu Ile Lys
Glu Leu Leu Asp Ile1 5 10 15Ala Glu Lys Tyr Gly Gly Val Ile Thr Ile
Phe Ala Lys Cys Lys Val 20 25 30Tyr Tyr Glu Gly Arg Ala Lys Ser Glu
Leu Gly Glu Gly Asp Arg Ile 35 40 45Ile Ile Ile Lys Pro Asp Gly Ser
Phe Leu Ile His Gln Asn Lys Lys 50 55 60Arg Glu Pro Val Asn Trp Gln
Pro Pro Gly Ser Ser Val Arg Ile Glu65 70 75 80Gly Asn Thr Ile Ile
Ser Ile Arg Arg Lys Pro Arg Glu Lys Leu Glu 85 90 95Val Glu Val Leu
Glu Ala Tyr Ser Gly Ile Val Phe Phe Ala Glu Asp 100 105 110Tyr Glu
Glu Leu Ala Leu Thr Gly Ser Glu Ala Glu Met Ala Glu Leu 115 120
125Ile Phe Gln Asn Pro Asp Ile Ile Glu Lys Gly Phe Lys Pro Leu Phe
130 135 140Arg Glu Lys Pro Ile Lys His Gly Ile Val Asp Ile Leu Gly
Val Asp145 150 155 160Lys Glu Gly Asn Ile Val Val Leu Glu Leu Lys
Arg Arg Arg Ala Asp 165 170 175Leu His Ala Val Ser Gln Leu Lys Arg
Tyr Val Glu Ser Leu Lys Glu 180 185 190Glu Tyr Lys Arg Lys Val Arg
Gly Ile Leu Val Ala Pro Ser Leu Thr 195 200 205Glu Gly Ala Arg Lys
Leu Leu Glu Lys Glu Gly Leu Glu Phe Lys Arg 210 215 220Leu Glu Pro
Pro Lys Arg Lys Asp Lys Lys Ser Arg Gly Lys Gln Lys225 230 235
240Thr Leu Asp Phe Phe 24565247PRTThermococcus onnurineus 65Lys Val
Glu Ala Val Thr Asn Pro Ser Arg Glu Glu Leu Leu Gly Ile1 5 10 15Ile
Asp Ser Ala Leu Ser Lys Glu Ala Met Leu Thr Ile Phe Ala Arg 20 25
30Cys Lys Val His Tyr Asp Gly Arg Ala Lys Ser Glu Leu Gly Ser Gly
35 40 45Asp Arg Val Ile Leu Val Lys Pro Asp Gly Ala Phe Leu Ile His
Gln 50 55 60Ser Lys Lys Arg Glu Pro Val Asn Trp Gln Pro Pro Gly Ser
Phe Val65 70 75 80Thr Val Glu Glu Arg Asp Gly Ile Ile Val Leu Arg
Ser Val Arg Arg 85 90 95Lys Pro Lys Glu Ile Leu Glu Val Glu Leu Glu
Glu Val Tyr Leu Ala 100 105 110Ser Leu Phe Lys Ala Glu Asp Tyr Glu
Glu Leu Ala Leu Thr Gly Ser 115 120 125Glu Ala Glu Met Ala Glu Met
Ile Phe Lys Asn Pro Glu Leu Ile Glu 130 135 140Pro Gly Phe Arg Pro
Leu Phe Arg Glu Lys Ser Ile Gly His Gly Ile145 150 155 160Val Asp
Ile Leu Gly Arg Asp Arg Glu Gly Asn Leu Val Val Leu Glu 165 170
175Leu Lys Arg Arg Arg Ala Asp Leu His Ala Val Ser Gln Leu Lys Arg
180 185 190Tyr Val Glu Ala Leu Arg Ala Glu His Pro Ala Val Arg Gly
Ile Leu 195 200 205Val Ala Pro Ser Leu Thr Ser Gly Ala Lys Lys Leu
Leu Glu Lys Glu 210 215 220Gly Leu Glu Phe Arg Arg Val Gln Pro Pro
Lys Arg Glu Ser Val Thr225 230 235 240Lys Gly Arg Gln Thr Thr Leu
24566242PRTMethanobacterium formicicum 66Asn Pro Glu Thr Gln Arg
Val Leu Glu Ile Ile Asn Glu Gly Leu Ser1 5 10 15Lys Arg Ala Val Ile
Thr Ile Met Ala Ser Cys Arg Val Asn Tyr Asp 20 25 30Gly Arg Ala Val
Ser Arg Leu Gly Val Gly Asp Arg Ile Ile Leu Ile 35 40 45Lys Ser Asp
Gly Ser Phe Ile Ile His Gln Asp Arg Asn Leu Glu Pro 50 55 60Val Asn
Trp Gln Pro Pro Lys Thr Lys Val Thr Val Glu Thr Tyr Gln65 70 75
80Gly Met Val Lys Ile Arg Gly Val Arg Arg Asn Pro Ser Glu Ser Leu
85 90 95Glu Val Glu Ile Leu Gln Thr His Leu Ala Ser Tyr Phe Ile Gly
Glu 100 105 110Asp Val Glu Ser Leu Glu Leu Ala Gly Tyr Glu Ala Asn
Met Gly Asp 115 120 125Leu Ile Phe Lys Asp Pro Glu Val Val Glu Lys
Gly Phe Arg Pro Thr 130 135 140Ser Arg Glu Tyr His Thr Pro Gln Gly
Phe Ile Asp Ile Leu Gly Lys145 150 155 160Asp Gln Asn Gly Asn Ile
Thr Ile Leu Glu Leu Lys Ser Arg Lys Ala 165 170 175Gly Ile Asn Ala
Val Lys Gln Leu Arg Arg Tyr Val Asp Cys Phe Ser 180 185 190Asp His
Lys Glu Ala Val Arg Gly Val Leu Val Ala Pro Ser Ile Thr 195 200
205Asp Asp Ala Arg Gln Leu Leu Glu Glu Gln Lys Met Glu Phe Lys Ala
210 215 220Leu Glu Pro Pro Arg Glu Leu Gly Thr Asp Lys Val Val Thr
Leu Glu225 230 235 240Asn Phe67242PRTThermococcus 67Met Lys Val Glu
Ala Lys Leu Asn Pro Ser Lys Glu Glu Ile Ile Asp1 5 10 15Leu Phe Ser
Lys Gly Leu Ser Lys Glu Ala Ile Leu Thr Ile Phe Ala 20 25 30His Cys
Lys Val Ser Tyr Asn Gly Arg Ala Lys Ser Glu Leu Gly Pro 35 40 45Gly
Asp Arg Val Ile Ile Ile Lys Pro Asp Gly Ser Phe Leu Ile His 50 55
60Gln Lys Glu Lys Arg Glu Pro Val Asn Trp Gln Pro Pro Gly Ser Ser65
70 75 80Pro Ser Leu Ser Ala Gly Glu Asp Lys Leu Ile Leu Lys Ser Val
Arg 85 90 95Arg Lys Pro Lys Glu Thr Leu Glu Val Glu Leu Ile Asp Val
Tyr Leu 100 105 110Phe Ser Tyr Phe Gln Ala Glu Asp Tyr Glu Thr Leu
Ala Leu Met Gly 115 120 125Ser Glu Ala Glu Met Ala Asp Leu Ile Phe
Glu Asn Pro Glu Leu Ile 130 135 140Glu Asp Gly Phe Lys Pro Leu Phe
Lys Glu Lys Ser Ile Lys His Gly145 150 155 160Ile Val Asp Ile Leu
Gly Lys Asp Lys Asn Gly Asn Ile Val Val Leu 165 170 175Glu Phe Lys
Arg Arg Arg Ala Asp Leu His Ala Val Ser Gln Leu Lys 180 185 190Arg
Tyr Val Glu Thr Ile Lys Glu Glu Tyr Lys Asn Val Arg Gly Ile 195 200
205Leu Val Ala Pro Ser Leu Thr Ser Gly Ala Lys Arg Leu Leu Glu Lys
210 215 220Glu Gly Leu Glu Phe Lys Lys Leu Thr Pro Pro Lys Lys Glu
Lys Ser225 230 235 240Lys Lys68242PRTThermococcus sibiricus 68Met
Lys Val Glu Ala Lys Leu Asn Pro Ser Lys Glu Glu Ile Ile Asp1 5 10
15Leu Phe Ser Lys Gly Leu Ser Lys Glu Ala Ile Val Thr Ile Phe Ala
20 25 30His Cys Lys Val Ser Tyr Asn Gly Arg Ala Lys Ser Glu Leu Gly
Pro 35 40 45Gly Asp Arg Val Ile Ile Ile Lys Pro Asp Gly Ser Phe Leu
Ile His 50 55 60Gln Lys Glu Lys Arg Glu Pro Val Asn Trp Gln Pro Pro
Gly Ser Ser65 70 75 80Pro Ser Leu Ser Ala Gly Glu Asp Lys Leu Ile
Leu Lys Ser Val Arg 85 90 95Arg Lys Pro Lys Glu Thr Leu Glu Val Glu
Leu Ile Asp Val Tyr Leu 100 105 110Phe Ser Tyr Phe Gln Ala Glu Asp
Tyr Glu Thr Leu Ala Leu Met Gly 115 120 125Ser Glu Ala Glu Met Ala
Asp Leu Ile Phe Glu Asn Pro Glu Leu Ile 130 135 140Glu Asp Gly Phe
Lys Pro Leu Phe Lys Glu Lys Ser Ile Lys His Gly145 150 155 160Ile
Val Asp Ile Leu Gly Lys Asp Lys Asn Gly Asn Ile Val Val Leu 165 170
175Glu Phe Lys Arg Arg Arg Ala Asp Leu His Ala Val Ser Gln Leu Lys
180 185 190Arg Tyr Val Glu Thr Ile Lys Glu Glu Tyr Lys Asn Val Arg
Gly Ile 195 200 205Leu Val Ala Pro Ser Leu Thr Ser Gly Ala Lys Arg
Leu Leu Glu Lys 210 215 220Glu Gly Leu Glu Phe
Lys Lys Leu Thr Pro Pro Lys Lys Glu Lys Ser225 230 235 240Lys
Lys69242PRTThermococcus sibiricus 69Met Lys Val Glu Val Lys Leu Asn
Pro Ser Lys Glu Glu Val Ile Asp1 5 10 15Leu Phe Ser Arg Gly Leu Ser
Thr Glu Ala Ile Val Thr Ile Phe Ala 20 25 30Arg Cys Asn Val Ser Tyr
Asp Gly Arg Ala Lys Ser Glu Leu Gly Leu 35 40 45Gly Asp Arg Val Ile
Ile Ile Lys Pro Asp Gly Ser Phe Leu Ile His 50 55 60Gln Lys Glu Lys
Arg Glu Pro Val Asn Trp Gln Pro Pro Gly Ser Ser65 70 75 80Val Ser
Leu Lys Ile Gly Glu Asp Lys Leu Ile Leu Arg Ser Val Arg 85 90 95Arg
Lys Pro Lys Glu Ile Leu Glu Val Gly Leu Ile Asp Val Tyr Leu 100 105
110Leu Ser Tyr Phe Gln Ala Glu Asp Tyr Glu Ser Leu Ala Leu Met Gly
115 120 125Ser Glu Ala Glu Met Ala Asp Leu Val Phe Glu Asn Pro Glu
Leu Ile 130 135 140Glu Pro Gly Phe Lys Pro Ile Phe Lys Glu Lys Ser
Ile Arg His Gly145 150 155 160Ile Ile Asp Ile Leu Gly Lys Asp Lys
Asp Gly Asn Ile Val Val Leu 165 170 175Glu Phe Lys Arg Arg Arg Ala
Asp Leu His Ala Val Ser Gln Leu Lys 180 185 190Arg Tyr Val Glu Thr
Met Arg Glu Glu Tyr Lys Asn Val Arg Gly Ile 195 200 205Leu Val Ala
Pro Ser Leu Thr Ser Gly Ala Lys Arg Leu Leu Glu Lys 210 215 220Glu
Gly Leu Glu Phe Lys Lys Leu Thr Pro Pro Lys Lys Glu Lys Ser225 230
235 240Arg Lys70241PRTThermococcus zilligii 70Asn Pro Ser Pro Glu
Glu Ile Lys Leu Leu Val Asp Ser Ala Ile Ser1 5 10 15Ser Glu Ala Leu
Leu Thr Ile Phe Ala His Cys Arg Val Tyr Tyr Asp 20 25 30Gly Arg Ala
Lys Ser Glu Leu Gly Ser Gly Asp Arg Val Ile Ile Val 35 40 45Lys Pro
Asp Gly Ser Phe Leu Ile His Gln Ser Lys Lys Arg Glu Pro 50 55 60Val
Asn Trp Gln Pro Pro Gly Ser Val Val His Val Glu Leu Arg Glu65 70 75
80Lys Pro Val Leu Val Ser Val Arg Arg Lys Pro Pro Glu Thr Leu Glu
85 90 95Val Glu Leu Glu Glu Val Tyr Leu Ile Thr Val Phe His Ala Glu
Asp 100 105 110Tyr Glu Glu Leu Ala Leu Thr Gly Ser Glu Ala Glu Met
Ala Glu Leu 115 120 125Ile Phe Glu Gln Pro Glu Val Ile Glu Pro Gly
Phe Lys Pro Leu Tyr 130 135 140Arg Glu Lys Pro Val Lys His Gly Ile
Val Asp Val Leu Gly Val Asp145 150 155 160Arg Glu Gly Asn Ile Val
Val Leu Glu Leu Lys Arg Arg Arg Ala Asp 165 170 175Leu His Ala Val
Ser Gln Leu Lys Arg Tyr Val Glu Thr Leu Lys Glu 180 185 190Glu His
Gly Asn Val Arg Gly Ile Leu Val Ala Pro Ser Leu Thr Ser 195 200
205Gly Ala Arg Lys Leu Leu Glu Lys Glu Gly Leu Glu Phe Arg Lys Leu
210 215 220Gln Pro Pro Lys Gly Gly Lys Lys Ser Arg Gly Lys Gln Leu
Arg Leu225 230 235 240Phe71239PRTThermococcus kodakarensis KOD1
71Lys Val Thr Val Ile Thr Ser Pro Ser Thr Glu Glu Leu Val Ser Leu1
5 10 15Val Asn Ser Ala Leu Leu Glu Glu Ala Met Leu Thr Ile Phe Ala
Arg 20 25 30Cys Lys Val His Tyr Asp Gly Arg Ala Lys Ser Glu Leu Gly
Ser Gly 35 40 45Asp Arg Val Ile Ile Val Lys Pro Asp Gly Ser Phe Leu
Ile His Gln 50 55 60Ser Lys Lys Arg Glu Pro Val Asn Trp Gln Pro Pro
Gly Ser Arg Val65 70 75 80Arg Leu Glu Leu Arg Glu Asn Pro Val Leu
Val Ser Ile Arg Arg Lys 85 90 95Pro Arg Glu Thr Leu Glu Val Glu Leu
Glu Glu Val Tyr Met Val Ser 100 105 110Val Phe Arg Ala Glu Asp Tyr
Glu Glu Leu Ala Leu Thr Gly Ser Glu 115 120 125Ala Glu Met Ala Glu
Leu Ile Phe Glu Asn Pro Glu Val Ile Glu Pro 130 135 140Gly Phe Lys
Pro Leu Phe Arg Glu Lys Ala Ile Gly Thr Gly Ile Val145 150 155
160Asp Val Leu Gly Arg Asp Ser Asp Gly Asn Ile Val Val Leu Glu Leu
165 170 175Lys Arg Arg Arg Ala Glu Leu His Ala Val Arg Gln Leu Lys
Ser Tyr 180 185 190Val Glu Ile Leu Arg Glu Glu Tyr Gly Asp Lys Val
Arg Gly Ile Leu 195 200 205Val Ala Pro Ser Leu Thr Ser Gly Ala Lys
Arg Leu Leu Glu Lys Glu 210 215 220Gly Leu Glu Phe Arg Lys Leu Glu
Pro Pro Lys Arg Asp Ser Lys225 230 23572239PRTThermococcus
kodakarensis KOD1 72Lys Val Thr Val Ile Thr Ser Pro Ser Thr Glu Glu
Leu Val Ser Leu1 5 10 15Val Asn Ser Ala Leu Leu Glu Glu Ala Met Leu
Thr Ile Phe Ala Arg 20 25 30Cys Lys Val His Tyr Asp Gly Arg Ala Lys
Ser Glu Leu Gly Ser Gly 35 40 45Asp Arg Val Ile Ile Val Lys Pro Asp
Gly Ser Phe Leu Ile His Gln 50 55 60Ser Lys Lys Arg Glu Pro Val Asn
Trp Gln Pro Pro Gly Ser Arg Val65 70 75 80Arg Leu Glu Leu Arg Glu
Asn Pro Val Leu Val Ser Ile Arg Arg Lys 85 90 95Pro Arg Glu Thr Leu
Glu Val Glu Leu Glu Glu Val Tyr Met Val Ser 100 105 110Val Phe Arg
Ala Glu Asp Tyr Glu Glu Leu Ala Leu Thr Gly Ser Glu 115 120 125Ala
Glu Met Ala Glu Leu Ile Phe Glu Asn Pro Glu Val Ile Glu Pro 130 135
140Gly Phe Lys Pro Leu Phe Arg Glu Lys Ala Ile Gly Thr Gly Ile
Val145 150 155 160Ala Val Leu Gly Arg Asp Ser Asp Gly Asn Ile Val
Val Leu Glu Leu 165 170 175Lys Arg Arg Arg Ala Glu Leu His Ala Val
Arg Gln Leu Lys Ser Tyr 180 185 190Val Glu Ile Leu Arg Glu Glu Tyr
Gly Asp Lys Val Arg Gly Ile Leu 195 200 205Val Ala Pro Ser Leu Thr
Ser Gly Ala Lys Arg Leu Leu Glu Lys Glu 210 215 220Gly Leu Glu Phe
Arg Lys Leu Glu Pro Pro Lys Arg Asp Ser Lys225 230
23573241PRTThermococcus barophilus 73Pro Ser His Glu Glu Ile Ile
Glu Ile Leu Asp Lys Ala Leu Ser Val1 5 10 15Glu Ala Ile Ile Thr Leu
Phe Ala Tyr Cys Arg Val Phe Tyr Glu Gly 20 25 30Arg Ala Lys Ser Glu
Leu Gly Pro Gly Asp Arg Val Ile Ile Ile Lys 35 40 45Pro Asp Gly Ser
Phe Leu Ile His Gln Lys Asn Lys Arg Glu Pro Val 50 55 60Asn Trp Gln
Pro Pro Gly Ser Val Val Ser Ile Val Leu Glu Asp Gly65 70 75 80Arg
Ile Met Leu Arg Ser Val Arg Arg Lys Pro Lys Glu Thr Leu Glu 85 90
95Val Glu Leu Ile Lys Thr Tyr Leu Val Ser Tyr Phe Gln Ala Glu Asp
100 105 110Tyr Glu Glu Leu Thr Leu Thr Gly Ser Glu Ala Glu Met Ala
Asp Leu 115 120 125Ile Phe Glu Asn Pro Ser Leu Ile Glu Glu Gly Phe
Lys Pro Leu Phe 130 135 140Lys Glu Lys Pro Ile Lys His Gly Ile Val
Asp Val Leu Gly Lys Asp145 150 155 160Lys His Gly Asn Leu Val Val
Leu Glu Leu Lys Arg Arg Arg Ala Asp 165 170 175Leu His Ala Val Ser
Gln Leu Lys Arg Tyr Val Asp Ser Leu Arg Glu 180 185 190Glu His Lys
Asn Val Arg Gly Ile Leu Val Ala Pro Ser Leu Thr Ala 195 200 205Gly
Ala Lys Lys Leu Leu Glu Lys Glu Gly Leu Glu Phe Lys Lys Leu 210 215
220Asn Pro Pro Lys Arg Glu Lys Arg Lys Lys Gly Lys Gln Lys Thr
Leu225 230 235 240Asp74239PRTMethanocaldococcus jannaschii 74Leu
Thr Asn Pro Thr Thr Lys Asp Leu Glu Asn Phe Ile Asp Met Tyr1 5 10
15Val Phe Lys Tyr Ile Leu Ile Leu Leu Ala Arg Cys Lys Val Phe Tyr
20 25 30Glu Gly Arg Ala Lys Ser Gln Leu Glu Glu Gly Asp Arg Val Ile
Ile 35 40 45Ile Lys Pro Asp Gly Ala Phe Leu Ile His Lys Asp Lys Lys
Arg Glu 50 55 60Pro Val Asn Trp Gln Pro Ser Gly Ser Ser Ile Ile Trp
Glu Val Glu65 70 75 80Asp Asn Phe Phe Ile Leu Lys Ser Ile Arg Arg
Lys Pro Lys Glu Glu 85 90 95Leu Lys Val Val Ile Ser Glu Val Tyr His
Ala Cys Ala Phe Asn Cys 100 105 110Glu Asp Tyr Glu Glu Ile Asn Leu
Arg Gly Ser Glu Ser Glu Met Ala 115 120 125Glu Met Ile Phe Arg Asn
Pro Asp Leu Ile Glu Glu Gly Phe Lys Pro 130 135 140Ile Ser Arg Glu
Tyr Gln Ile Pro Thr Gly Ile Val Asp Ile Leu Gly145 150 155 160Lys
Asp Lys Glu Asn Lys Trp Val Ile Leu Glu Leu Lys Arg Arg Arg 165 170
175Ala Asp Leu Gln Ala Val Ser Gln Leu Lys Arg Tyr Val Glu Tyr Phe
180 185 190Lys Asn Lys Tyr Gly Glu Asp Lys Val Arg Gly Ile Leu Val
Ser Pro 195 200 205Ser Leu Thr Thr Gly Ala Glu Lys Leu Leu Lys Glu
Glu Asn Leu Glu 210 215 220Phe Lys Arg Leu Asn Pro Pro Lys Gly Ser
Lys Arg Asp Leu Lys225 230 23575239PRTMethanococcus jannaschii
75Leu Thr Asn Pro Thr Thr Lys Asp Leu Glu Asn Phe Ile Asp Met Tyr1
5 10 15Val Phe Lys Tyr Ile Leu Ile Leu Leu Ala Arg Cys Lys Val Phe
Tyr 20 25 30Glu Gly Arg Ala Lys Ser Gln Leu Glu Glu Gly Asp Arg Val
Ile Ile 35 40 45Ile Lys Pro Asp Gly Ala Phe Leu Ile His Lys Asp Lys
Lys Arg Glu 50 55 60Pro Val Asn Trp Gln Pro Ser Gly Ser Ser Ile Ile
Trp Glu Val Glu65 70 75 80Asp Asn Phe Phe Ile Leu Lys Ser Ile Arg
Arg Lys Pro Lys Glu Glu 85 90 95Leu Lys Val Val Ile Ser Glu Val Tyr
His Ala Cys Ala Phe Asn Cys 100 105 110Glu Asp Tyr Glu Glu Ile Asn
Leu Arg Gly Ser Glu Ser Glu Met Ala 115 120 125Glu Met Ile Phe Arg
Asn Pro Asp Leu Ile Glu Glu Gly Phe Lys Pro 130 135 140Ile Ser Arg
Glu Tyr Gln Ile Pro Thr Gly Ile Val Asp Ile Leu Gly145 150 155
160Lys Asp Lys Glu Asn Lys Trp Val Ile Leu Glu Leu Lys Arg Arg Arg
165 170 175Ala Asp Leu Gln Ala Val Ser Gln Leu Lys Arg Tyr Val Glu
Tyr Phe 180 185 190Lys Asn Lys Tyr Gly Glu Asp Lys Val Arg Gly Ile
Leu Val Ser Pro 195 200 205Ser Leu Thr Thr Gly Ala Glu Lys Leu Leu
Lys Glu Glu Asn Leu Glu 210 215 220Phe Lys Arg Leu Asn Pro Pro Lys
Gly Ser Lys Arg Asp Leu Lys225 230 23576238PRTMethanocaldococcus
vulcanius 76Met Lys Val His Phe Leu His Lys Pro Asp Ile Lys Asn Leu
Val Asn1 5 10 15Phe Ile Lys Glu His Ile Tyr Asp Ser Val Ile Ile Leu
Leu Ser Arg 20 25 30Cys Ser Val Ile Tyr Asp Gly Arg Ala Lys Ser Thr
Leu Asn Glu Gly 35 40 45Asp Arg Ile Ile Met Ile Lys Pro Asp Gly Ser
Leu Leu Ile His Lys 50 55 60Asn Lys Lys Arg Glu Pro Val Asn Trp Gln
Pro Ser Gly Ser Ser Ile65 70 75 80Ser Tyr Lys Ile Glu Asn Lys Gln
Phe Ile Ile Arg Ser Ile Arg Lys 85 90 95Lys Pro Arg Glu Val Leu Glu
Ile Ile Val Tyr Glu Val Tyr His Ala 100 105 110Cys Ala Phe Lys Cys
Glu Asp Tyr Glu Glu Leu Asn Leu Thr Gly Ser 115 120 125Glu Gly Asp
Met Val Asp Met Ile Phe Lys Asn Pro Lys Leu Ile Glu 130 135 140Glu
Gly Phe Lys Pro Leu Ser Lys Glu Tyr Gln Ile Pro Thr Gly Ile145 150
155 160Ile Asp Ile Leu Gly Lys Asp Glu Asn Asn Asn Trp Val Ile Leu
Glu 165 170 175Leu Lys Arg Arg Arg Ala Asp Leu Gln Ser Val Ser Gln
Leu Lys Arg 180 185 190Tyr Val Glu Tyr Phe Lys Ser Lys Tyr Gly Glu
Lys Arg Val Arg Gly 195 200 205Ile Leu Val Ala Pro Ser Leu Thr Thr
Gly Ala Leu Asn Leu Leu Lys 210 215 220Ser Glu Asn Leu Glu Phe Lys
Lys Leu Thr Pro Pro Lys Lys225 230 23577235PRTThermococcus
paralvinellae 77Met Lys Val Glu Ala Lys Val Asp Pro Ser His Glu Glu
Met Val Glu1 5 10 15Ile Leu Asp Lys Ala Leu Ser Thr Asp Ala Ile Ile
Thr Leu Phe Ala 20 25 30Tyr Cys Arg Val Phe Tyr Glu Gly Arg Ala Lys
Ser Glu Leu Gly Pro 35 40 45Gly Asp Arg Val Ile Ile Ile Lys Pro Asp
Gly Ser Phe Leu Ile His 50 55 60Gln Lys Asn Lys Arg Glu Pro Val Asn
Trp Gln Pro Pro Gly Ser Ala65 70 75 80Val Ser Ile Val Leu Glu Asp
Gly Lys Ile Met Leu Arg Ser Val Arg 85 90 95Arg Lys Pro Lys Glu Thr
Leu Glu Val Glu Leu Ile Lys Thr Tyr Leu 100 105 110Val Ser Tyr Phe
Gln Ala Glu Asp Tyr Glu Glu Leu Ala Leu Thr Gly 115 120 125Ser Glu
Ala Glu Met Ala Asp Leu Ile Phe Glu Asn Pro Ser Leu Ile 130 135
140Glu Glu Gly Phe Lys Pro Leu Phe Lys Glu Lys Pro Ile Lys His
Gly145 150 155 160Ile Val Asp Val Leu Gly Arg Asp Lys His Gly Asn
Leu Val Val Leu 165 170 175Glu Leu Lys Arg Arg Arg Ala Asp Leu His
Ala Val Ser Gln Leu Lys 180 185 190Arg Tyr Val Asp Ala Leu Arg Glu
Glu His Lys Asn Val Arg Gly Ile 195 200 205Leu Val Ala Pro Ser Ile
Thr Ala Gly Ala Lys Lys Leu Leu Glu Lys 210 215 220Glu Gly Leu Glu
Phe Lys Lys Leu Asn Pro Pro225 230 23578235PRTThermococcus
paralvinellae 78Met Lys Val Glu Ala Lys Val Asp Pro Ser His Glu Glu
Met Val Glu1 5 10 15Ile Leu Asp Lys Ala Leu Ser Thr Asp Ala Ile Ile
Thr Leu Phe Ala 20 25 30Tyr Cys Arg Val Phe Tyr Glu Gly Arg Ala Lys
Ser Glu Leu Gly Pro 35 40 45Gly Asp Arg Val Ile Ile Ile Lys Pro Asp
Gly Ser Phe Leu Ile His 50 55 60Gln Lys Asn Lys Arg Glu Pro Val Asn
Trp Gln Pro Pro Gly Ser Ala65 70 75 80Val Ser Ile Val Leu Glu Asp
Gly Lys Ile Met Leu Arg Ser Val Arg 85 90 95Arg Lys Pro Lys Glu Thr
Leu Glu Val Glu Leu Ile Lys Thr Tyr Leu 100 105 110Val Ser Tyr Phe
Gln Ala Glu Asp Tyr Glu Glu Leu Ala Leu Thr Gly 115 120 125Ser Glu
Ala Glu Met Ala Asp Leu Ile Phe Glu Asn Pro Ser Leu Ile 130 135
140Glu Glu Gly Phe Lys Pro Leu Phe Lys Glu Lys Pro Ile Lys His
Gly145 150 155 160Ile Val Asp Val Leu Gly Arg Asp Lys His Gly Asn
Leu Val Val Leu 165 170 175Glu Leu Lys Arg Arg Arg Ala Asp Leu His
Ala Val Ser Gln Leu Lys 180 185 190Arg Tyr Val Asp Ala Leu Arg Glu
Glu His Lys Asn Val Arg Gly Ile 195 200 205Leu Val Ala Pro Ser Ile
Thr Ala Gly Ala Lys Lys Leu Leu Glu Lys 210 215 220Glu Gly Leu Glu
Phe Lys Lys Leu Asn Pro Pro225 230 23579237PRTHalomicrobium
mukohataei 79Glu Thr Leu Ser Asp Pro Asp Phe Asp Ala Ala Gly Asp
Leu Val Glu1 5 10 15Arg Gly Ile Asp Ala Gly Ala Leu Val Thr Leu Phe
Gly Arg Cys Arg 20 25
30Val Asp Tyr Asp Gly Arg Ala Thr Ser Thr Leu Gly Pro Gly Asp Arg
35 40 45His Val Met Leu Lys Pro Asp Gly Ala Ala Leu Val His Thr Asp
Glu 50 55 60Gly Gln Gln Pro Val Asn Trp Gln Pro Pro Gly Cys Glu His
Ala Val65 70 75 80Arg Val Val Asp Gly Glu Phe Val Val Glu Ser Glu
Arg Ser Ser Pro 85 90 95Asp Glu Leu Leu Ser Ile Ala Phe Glu Ser Leu
Ser His Val Gly Val 100 105 110Phe Asp Val Thr Asp Ala Thr Asp Leu
Ser Leu Thr Gly Thr Glu Glu 115 120 125Asp Leu Arg Glu Arg Ile Leu
Asp Asp Pro Asp Leu Leu Glu Pro Ala 130 135 140Phe Thr Pro Leu Ala
Thr Glu Arg Ser Thr Pro Ala Gly Ala Ile Asp145 150 155 160Ile Tyr
Gly Glu Asp Ala Asp Gly Arg Thr Val Val Val Glu Leu Lys 165 170
175Arg Arg Arg Val Gly Pro Asp Ala Val Gly Gln Leu Asp Arg Tyr Val
180 185 190Gln Ala Leu Gly Arg Asp Leu His Asp Glu Ala Glu Ile Arg
Gly Leu 195 200 205Leu Val Ala Pro Ser Val Thr Asp Arg Ala Arg Glu
Leu Leu Ala Gln 210 215 220Lys Gly Leu Glu Phe Val Ser Leu Ala Pro
Pro Glu Glu225 230 23580235PRTHalomicrobium katesii 80Leu Ser Asp
Pro Asp Phe Asp Ala Ala Gly Asp Leu Val Glu Arg Gly1 5 10 15Ile Asp
Ala Gly Ala Leu Val Thr Leu Phe Gly Arg Cys Arg Val Asp 20 25 30Tyr
Asp Gly Arg Ala Thr Ser Thr Leu Gly Pro Gly Asp Arg His Val 35 40
45Met Leu Lys Pro Asp Gly Ala Ala Leu Val His Thr Asp Glu Gly Gln
50 55 60Gln Pro Val Asn Trp Gln Pro Pro Gly Cys Glu His Ala Val Arg
Val65 70 75 80Val Asp Gly Glu Phe Val Val Glu Ser Glu Arg Ser Ser
Pro Asp Glu 85 90 95Leu Leu Ser Ile Ala Phe Glu Ser Leu Ala His Val
Gly Val Phe Asp 100 105 110Val Thr Asp Ala Thr Asp Leu Ser Leu Thr
Gly Thr Glu Ala Asp Leu 115 120 125Arg Glu Arg Ile Leu Asp Asp Pro
Asp Leu Leu Glu Pro Ala Phe Thr 130 135 140Pro Leu Ala Thr Glu Arg
Ser Thr Pro Ala Gly Ala Ile Asp Ile Tyr145 150 155 160Gly Glu Asp
Gly Asp Gly Arg Thr Val Val Val Glu Leu Lys Arg Arg 165 170 175Arg
Val Gly Pro Asp Ala Val Gly Gln Leu Asp Arg Tyr Val Gln Ala 180 185
190Leu Gly Arg Asp Leu His Asp Glu Ala Glu Ile Arg Gly Ile Leu Val
195 200 205Ala Pro Ser Val Thr Asp Arg Ala Arg Glu Leu Leu Ala Gln
Lys Gly 210 215 220Leu Glu Phe Val Ser Leu Ala Pro Pro Glu Glu225
230 23581235PRTHaloarcula japonica 81Glu Thr Leu Thr Tyr Pro Asp
Pro Ala Asp Ala Leu Asp Leu Ala Ser1 5 10 15Arg Asn Ala Asp Arg Gly
Ala Leu Val Thr Leu Val Gly Thr Cys Thr 20 25 30Val Glu Tyr Glu Gly
Arg Ala Ala Ser Ser Leu Gly Leu Gly Asp Arg 35 40 45His Val Met Leu
Lys Pro Asp Gly Ala Ala Leu Val His Thr Asp Glu 50 55 60Gly Gln Gln
Pro Val Asn Trp Gln Pro Pro Gly Cys Glu His Ser Ile65 70 75 80Ser
Val Asp Asp Gly Ser Leu Val Val Arg Ser Thr Arg Ser Thr Pro 85 90
95Glu Glu Leu Leu Glu Val Thr Phe Glu Thr Val Ala His Ala Ala Ala
100 105 110Phe Asp Val Thr Asp Ser Lys Asp Leu Ala Leu Thr Gly Thr
Glu Ala 115 120 125Asp Leu Lys Asp Arg Ile Leu Asp Glu Pro Gly Leu
Val Glu Ser Gly 130 135 140Phe Thr Pro Leu Ala Thr Glu Arg Glu Thr
Pro Ala Gly Ala Val Asp145 150 155 160Ile Tyr Gly Glu Asp Ala Asp
Gly Arg Thr Thr Ile Leu Glu Leu Lys 165 170 175Arg Arg Arg Val Gly
Pro Asp Ala Val Gly Gln Leu Gly Arg Tyr Val 180 185 190Asp Ala Leu
Glu Arg Asp Leu His Ala Asp Thr Glu Val Arg Gly Ile 195 200 205Leu
Val Ala Pro Ser Val Thr Asp Arg Ala Arg Gln Leu Leu Ala Glu 210 215
220Lys Gly Leu Glu Phe Val Ser Leu Glu Pro Pro225 230
23582226PRTPyrococcus furiosus 82Glu Asn Pro Arg Ile Glu Glu Ile
Lys Glu Leu Leu Glu Val Ala Glu1 5 10 15Ser Arg Glu Gly Leu Leu Thr
Ile Phe Ala Arg Cys Thr Val Tyr Tyr 20 25 30Glu Gly Arg Ala Lys Ser
Glu Leu Gly Glu Gly Asp Arg Ile Ile Ile 35 40 45Ile Lys Pro Asp Gly
Ser Phe Leu Ile His Gln Lys Lys Lys Arg Glu 50 55 60Pro Val Asn Trp
Gln Pro Pro Gly Ser Lys Val Lys Met Glu Gly Asn65 70 75 80Ser Leu
Ile Ser Ile Arg Arg Asn Pro Lys Glu Thr Leu Lys Val Asp 85 90 95Ile
Ile Glu Ala Tyr Ala Ala Val Leu Phe Met Ala Glu Asp Tyr Glu 100 105
110Glu Leu Thr Leu Thr Gly Ser Glu Ala Glu Met Ala Glu Leu Ile Phe
115 120 125Gln Asn Pro Asn Val Ile Glu Glu Gly Phe Lys Pro Met Phe
Arg Glu 130 135 140Lys Pro Ile Lys His Gly Ile Val Asp Val Leu Gly
Val Asp Arg Glu145 150 155 160Gly Asn Ile Val Val Leu Glu Leu Lys
Arg Arg Arg Ala Asp Leu His 165 170 175Ala Val Ser Gln Leu Lys Arg
Tyr Val Asp Ala Leu Lys Glu Glu His 180 185 190Gly Asn Lys Val Arg
Gly Ile Leu Val Ala Pro Ser Leu Thr Glu Gly 195 200 205Ala Lys Lys
Leu Leu Glu Lys Leu Gly Leu Glu Phe Arg Lys Leu Glu 210 215 220Pro
Pro22583226PRTPyrococcus furiosus 83Glu Asn Pro Arg Ile Glu Glu Ile
Lys Glu Leu Leu Glu Val Ala Glu1 5 10 15Ser Arg Glu Gly Leu Leu Thr
Ile Phe Ala Arg Cys Thr Val Tyr Tyr 20 25 30Glu Gly Arg Ala Lys Ser
Glu Leu Gly Glu Gly Asp Arg Ile Ile Ile 35 40 45Ile Lys Pro Asp Gly
Ser Phe Leu Ile His Gln Lys Lys Lys Arg Glu 50 55 60Pro Val Asn Trp
Gln Pro Pro Gly Ser Lys Val Lys Met Glu Gly Asn65 70 75 80Ser Leu
Ile Ser Ile Arg Arg Asn Pro Lys Glu Thr Leu Lys Val Asp 85 90 95Ile
Ile Glu Ala Tyr Ala Ala Val Leu Phe Met Ala Glu Asp Tyr Glu 100 105
110Glu Leu Thr Leu Thr Gly Ser Glu Ala Glu Met Ala Glu Leu Ile Phe
115 120 125Gln Asn Pro Asn Val Ile Glu Glu Gly Phe Lys Pro Met Phe
Arg Glu 130 135 140Lys Pro Ile Lys His Gly Ile Val Asp Val Leu Gly
Val Asp Arg Glu145 150 155 160Gly Asn Ile Val Val Leu Glu Leu Lys
Arg Arg Arg Ala Asp Leu His 165 170 175Ala Val Ser Gln Leu Lys Arg
Tyr Val Asp Ala Leu Lys Glu Glu His 180 185 190Gly Asn Lys Val Arg
Gly Ile Leu Val Ala Pro Ser Leu Thr Glu Gly 195 200 205Ala Lys Lys
Leu Leu Glu Lys Leu Gly Leu Glu Phe Arg Lys Leu Glu 210 215 220Pro
Pro22584226PRTPyrococcus furiosus DSM 3638 84Glu Asn Pro Arg Ile
Glu Glu Ile Lys Glu Leu Leu Glu Val Ala Glu1 5 10 15Ser Arg Glu Gly
Leu Leu Thr Ile Phe Ala Arg Cys Thr Val Tyr Tyr 20 25 30Glu Gly Arg
Ala Lys Ser Glu Leu Gly Glu Gly Asp Arg Ile Ile Ile 35 40 45Ile Lys
Pro Asp Gly Ser Phe Leu Ile His Gln Lys Lys Lys Arg Glu 50 55 60Pro
Val Asn Trp Gln Pro Pro Gly Ser Lys Val Lys Met Glu Gly Asn65 70 75
80Ser Leu Ile Ser Ile Arg Arg Asn Pro Lys Glu Thr Leu Lys Val Asp
85 90 95Ile Ile Glu Ala Tyr Ala Ala Val Leu Phe Met Ala Glu Asp Tyr
Glu 100 105 110Glu Leu Thr Leu Thr Gly Ser Glu Ala Glu Met Ala Glu
Leu Ile Phe 115 120 125Gln Asn Pro Asn Val Ile Glu Glu Gly Phe Lys
Pro Met Phe Arg Glu 130 135 140Lys Pro Ile Lys His Gly Ile Val Asp
Val Leu Gly Val Asp Arg Glu145 150 155 160Gly Asn Ile Val Val Leu
Glu Leu Lys Arg Arg Arg Ala Asp Leu His 165 170 175Ala Val Ser Gln
Leu Lys Arg Tyr Val Asp Ala Leu Lys Glu Glu His 180 185 190Gly Asn
Lys Val Arg Gly Ile Leu Val Ala Pro Ser Leu Thr Glu Gly 195 200
205Ala Lys Lys Leu Leu Glu Lys Leu Gly Leu Glu Phe Arg Lys Leu Glu
210 215 220Pro Pro22585226PRTPyrococcus furiosus 85Glu Asn Pro Arg
Ile Glu Glu Ile Lys Glu Leu Leu Glu Val Ala Glu1 5 10 15Ser Arg Glu
Gly Leu Leu Thr Ile Phe Ala Arg Cys Thr Val Tyr Tyr 20 25 30Glu Gly
Arg Ala Lys Ser Glu Leu Gly Glu Gly Asp Arg Ile Ile Ile 35 40 45Ile
Lys Pro Asp Gly Ser Phe Leu Ile His Gln Lys Lys Lys Arg Glu 50 55
60Pro Val Asn Trp Gln Pro Pro Gly Ser Lys Val Lys Met Glu Gly Asn65
70 75 80Ser Leu Ile Ser Ile Arg Arg Asn Pro Lys Glu Thr Leu Lys Val
Asp 85 90 95Ile Ile Glu Ala Tyr Ala Ala Val Leu Phe Met Ala Glu Asp
Tyr Glu 100 105 110Glu Leu Thr Leu Thr Gly Ser Glu Ala Glu Met Ala
Glu Leu Ile Phe 115 120 125Gln Asn Pro Asn Val Ile Glu Glu Gly Phe
Lys Pro Met Phe Arg Glu 130 135 140Lys Pro Ile Lys His Gly Ile Val
Asp Val Leu Gly Val Asp Arg Glu145 150 155 160Gly Asn Ile Val Val
Leu Glu Leu Lys Arg Arg Arg Ala Asp Leu His 165 170 175Ala Val Ser
Gln Leu Lys Arg Tyr Val Asp Ala Leu Lys Glu Glu His 180 185 190Gly
Asn Lys Val Arg Gly Ile Leu Val Ala Pro Ser Leu Thr Glu Gly 195 200
205Ala Lys Lys Leu Leu Glu Lys Leu Gly Leu Glu Phe Arg Lys Leu Glu
210 215 220Pro Pro22586232PRTHalostagnicola larsenii 86Leu Glu Arg
Pro Ala Val Glu Thr Ala Cys Glu Thr Val Ala Asp Gly1 5 10 15Ile Asp
Arg Asp Ala Leu Val Thr Val Phe Gly Arg Cys Ser Val Glu 20 25 30Tyr
Asp Gly Arg Ala Ser Ser Gln Leu Asp Ala Gly Asp Arg His Val 35 40
45Met Cys Lys Pro Asp Gly Thr Thr Leu Val His Thr Asp Glu Gly Gln
50 55 60Gln Pro Val Asn Trp Gln Pro Pro Gly Cys Thr His Glu Val Phe
Cys65 70 75 80Asp Asp Gly Ala Leu Phe Leu Glu Ser His Arg Ser Thr
Pro Gln Glu 85 90 95Arg Leu Leu Ile Gly Phe Glu Arg Val Val His Val
Ser Val Phe Pro 100 105 110Val Ser Asp Ser Ser Glu Leu Thr Leu Val
Gly Thr Glu Glu Asp Leu 115 120 125Arg Gln Arg Ile Leu Glu Asp Pro
Gly Leu Leu Glu Pro Gly Phe Arg 130 135 140Pro Leu Ala Thr Glu Arg
Asp Thr Pro Ala Gly Ala Ile Asp Ile Tyr145 150 155 160Gly Glu Asp
Ser Val Gly Arg Ala Val Val Val Glu Leu Lys Arg Arg 165 170 175Arg
Val Gly Pro Asp Ala Val Ser Gln Leu Arg Arg Tyr Val Asp Ala 180 185
190Leu Glu Arg Asp Leu His Ala Asp Ala Ser Ile Arg Gly Ile Leu Val
195 200 205Ala Pro Ser Val Thr Asp Arg Ala Ser Gly Leu Leu Gly Glu
His Gly 210 215 220Leu Glu Phe Val Ser Leu Glu Pro225
23087222PRTThermococcus barophilus 87Ile Thr Leu Phe Ala Tyr Cys
Arg Val Phe Tyr Glu Gly Arg Ala Lys1 5 10 15Ser Glu Leu Gly Pro Gly
Asp Arg Val Ile Ile Ile Lys Pro Asp Gly 20 25 30Ser Phe Leu Ile His
Gln Lys Asn Lys Arg Glu Pro Val Asn Trp Gln 35 40 45Pro Pro Gly Ser
Val Val Ser Ile Val Leu Gly Asp Gly Arg Ile Met 50 55 60Leu Arg Ser
Val Arg Arg Lys Pro Lys Glu Thr Leu Glu Val Glu Leu65 70 75 80Ile
Lys Thr Tyr Leu Val Ser Tyr Phe Gln Ala Glu Asp Tyr Glu Glu 85 90
95Leu Thr Leu Thr Gly Ser Glu Ala Glu Met Ala Asp Leu Ile Phe Glu
100 105 110Asn Pro Ser Leu Ile Glu Glu Gly Phe Lys Pro Leu Phe Lys
Glu Lys 115 120 125Pro Ile Lys His Gly Ile Val Asp Val Leu Gly Lys
Asp Lys His Gly 130 135 140Asn Leu Val Val Leu Glu Leu Lys Arg Arg
Arg Ala Asp Leu His Ala145 150 155 160Val Ser Gln Leu Lys Arg Tyr
Val Asp Ser Leu Arg Glu Glu His Lys 165 170 175Asn Val Arg Gly Ile
Leu Val Ala Pro Ser Leu Thr Ala Gly Ala Lys 180 185 190Lys Leu Leu
Glu Lys Glu Gly Leu Glu Phe Lys Lys Leu Asn Pro Pro 195 200 205Lys
Arg Glu Lys Arg Lys Lys Gly Lys Gln Lys Thr Leu Asp 210 215
22088221PRTPyrococcus 88Phe Glu Lys Pro Ser Ile Glu Glu Val Lys Glu
Leu Phe Lys Met Ala1 5 10 15Glu Lys His Gly Gly Val Val Thr Val Phe
Ala Arg Cys Lys Val Tyr 20 25 30Tyr Glu Gly Arg Ala Lys Ser Glu Leu
Gly Glu Gly Asp Arg Ile Ile 35 40 45Ile Val Lys Pro Asp Gly Thr Phe
Leu Val His Gln Asn Lys Lys Arg 50 55 60Glu Pro Val Asn Trp Gln Pro
Pro Gly Ser Ile Val Ser Ile Glu Gly65 70 75 80Asn Ser Ile Ile Ser
Ile Arg Arg Arg Pro Arg Glu Lys Leu Glu Val 85 90 95Glu Leu Ile Asp
Val Tyr Ala Val Val Val Phe Leu Ala Glu Asp Tyr 100 105 110Lys Glu
Leu Ala Leu Thr Gly Ser Glu Ala Glu Met Ala Lys Leu Ile 115 120
125Phe Glu Asn Pro Glu Val Ile Glu Glu Gly Phe Lys Pro Met Phe Arg
130 135 140Glu Lys Pro Ile Lys His Gly Ile Val Asp Ile Met Gly Val
Asp Lys145 150 155 160Asn Gly Asn Ile Val Ile Leu Glu Leu Lys Arg
Arg Lys Ala Asp Leu 165 170 175His Ala Val Ser Gln Leu Lys Arg Tyr
Val Asp Ala Leu Lys Glu Glu 180 185 190Tyr Gly Glu Arg Val Arg Gly
Ile Leu Val Ala Pro Ser Leu Thr Glu 195 200 205Gly Ala Lys Lys Leu
Leu Glu Lys Glu Gly Leu Glu Phe 210 215 22089221PRTThermococcus
cleftensis 89Leu Thr Ile Phe Ala Arg Cys Arg Val His Tyr Asp Gly
Arg Ala Lys1 5 10 15Ser Glu Leu Gly Ser Gly Asp Arg Val Ile Leu Val
Lys Pro Asp Gly 20 25 30Ala Phe Leu Val His Gln Ser Lys Lys Arg Glu
Pro Val Asn Trp Gln 35 40 45Pro Pro Gly Ser Phe Val Thr Val Glu Val
Arg Glu Gly Leu Val Val 50 55 60Leu Arg Ser Val Arg Arg Lys Pro Lys
Glu Ile Leu Glu Val Glu Leu65 70 75 80Glu Glu Val Tyr Leu Ala Ser
Leu Phe Asn Ala Glu Asp Tyr Glu Glu 85 90 95Leu Ala Leu Thr Gly Ser
Glu Ala Glu Met Ala Glu Met Ile Phe Arg 100 105 110Asn Pro Glu Leu
Ile Glu Pro Gly Phe Lys Pro Leu Phe Arg Glu Lys 115 120 125Gln Ile
Gly His Gly Ile Val Asp Ile Leu Gly Lys Asp Gly Arg Gly 130 135
140Asn Leu Val Val Leu Glu Leu Lys Arg Arg Lys Ala Asp Leu His
Ala145 150 155 160Val Ser Gln Leu Lys Arg Tyr Val Glu Ala Leu Glu
Arg Glu His Gly 165 170 175Lys Val Arg Gly Ile Leu Val Ala Pro Ser
Leu Thr Ser Gly Ala Lys 180 185 190Lys Leu Leu Glu Lys Glu
Gly Leu Glu Phe Arg Lys Val Glu Pro Pro 195 200 205Lys Lys Glu Lys
Leu Gly Arg Gly Arg Gln Lys Thr Leu 210 215 22090224PRTPalaeococcus
pacificus 90Ile Thr Leu Phe Ala His Cys Ser Val Phe Tyr Asp Gly Arg
Ala Lys1 5 10 15Ser Glu Leu Gly Ala Gly Asp Arg Val Ile Met Ile Lys
Pro Asp Gly 20 25 30Thr Phe Leu Ile His Gln Lys Glu Lys Arg Val Pro
Val Asn Trp Gln 35 40 45Pro Pro Gly Ser Ile Val Ser Phe Gln Ile Glu
Glu Gly Lys Ile Lys 50 55 60Leu Arg Ser Val Arg Arg Lys Pro Lys Glu
Ile Leu Glu Val Glu Leu65 70 75 80Leu Lys Val Tyr Leu Ile Ser Tyr
Phe Gln Ala Glu Asp Tyr Glu Ala 85 90 95Leu Asn Leu Met Gly Ser Glu
Ala Glu Met Ala Asp Leu Ile Leu Gln 100 105 110Asn Pro Ser Ile Ile
Glu Glu Gly Phe Lys Ala Leu Gln Lys Glu Lys 115 120 125Pro Ile Lys
His Gly Ile Ile Asp Ile Tyr Gly Val Asp Arg Asp Gly 130 135 140Asn
Ile Val Val Leu Glu Leu Lys Arg Arg Arg Ala Asp Leu His Ala145 150
155 160Val Ser Gln Leu Lys Arg Tyr Val Asp Ala Leu Lys Glu Glu His
Gly 165 170 175Ser Val Arg Gly Ile Leu Val Ala Pro Ser Leu Thr Ser
Gly Ala Glu 180 185 190Lys Leu Leu Lys Asp Leu Gly Leu Glu Phe Lys
Lys Leu Asn Pro Pro 195 200 205Lys Arg Glu Lys Ala Arg Lys Gly Lys
Gln Lys Thr Leu Asp Met Leu 210 215 22091222PRTMethanosphaera
stadtmanae 91Ile Ile Ile Leu Ala Gln Cys His Val Glu Tyr Glu Gly
Arg Ala Arg1 5 10 15Ser Arg Leu Asp Lys Gly Asp Arg Leu Ile Leu Ile
Lys Lys Asp Gly 20 25 30Thr Phe Thr Ile His Gln Glu Leu Asn Leu Asp
Pro Val Asn Trp Gln 35 40 45Ala Pro Gly Cys Lys Asn Lys Val Ser Leu
Lys Glu Asn Gln Ile Ile 50 55 60Leu Gln Ser Ile Lys Thr Lys Pro Asp
Glu Glu Ile Thr Val Tyr Leu65 70 75 80Asp Thr Val Tyr Cys Ala Thr
Tyr Tyr Asn Cys Val Asp Thr Lys Asn 85 90 95Leu Glu Ile Arg Gly Tyr
Glu Lys His Met Val Asp Leu Ala Trp Glu 100 105 110Lys Pro Glu Leu
Ile Glu Lys Gly Phe Arg Pro Thr Arg Arg Glu Tyr 115 120 125Gln Thr
Glu Asn Gly Phe Ile Asp Leu Met Gly Thr Asp Lys Asp Glu 130 135
140Lys Leu Met Ile Leu Glu Phe Lys Ser Arg Lys Ala Gly Thr Asn
Ala145 150 155 160Val Lys Gln Leu Lys Gly Tyr Val Glu Cys Phe Met
Asp Asn Lys Glu 165 170 175Phe Val Arg Gly Ile Ile Val Ala Pro Asp
Ile Thr Asp Asn Ala Leu 180 185 190Glu Leu Leu Lys Ser Leu Gln Met
Glu Phe Ile Pro Leu Asn Pro Pro 195 200 205Lys Asp Leu Leu Thr Lys
Lys Ala Ser Thr Leu Asp Ser Phe 210 215
22092223PRTMethanocaldococcus 92Leu Ile Leu Leu Ala Arg Cys Arg Val
Phe Tyr Glu Gly Arg Ala Lys1 5 10 15Ser Gln Leu Glu Glu Gly Asp Arg
Val Ile Ile Ile Lys Pro Asp Gly 20 25 30Thr Phe Leu Ile His Lys Asp
Lys Lys Arg Glu Pro Val Asn Trp Gln 35 40 45Pro Pro Gly Ser Asn Ile
Ile Trp Lys Val Glu Asp Asn Tyr Phe Ile 50 55 60Leu Lys Ser Ile Arg
Arg Lys Pro Lys Glu Glu Leu Lys Val Val Ile65 70 75 80Ser Glu Val
Tyr His Thr Cys Ala Phe Asn Cys Glu Asp Tyr Glu Glu 85 90 95Leu Asn
Leu Thr Gly Ser Glu Ser Glu Met Ala Glu Met Ile Phe Arg 100 105
110Asn Pro Asn Leu Ile Glu Glu Gly Phe Lys Pro Leu Ser Arg Glu Tyr
115 120 125Gln Ile Pro Thr Gly Ile Ile Asp Ile Leu Gly Lys Asp Lys
Asp Glu 130 135 140Arg Trp Val Ile Leu Glu Leu Lys Arg Arg Arg Ala
Asp Leu Gln Ala145 150 155 160Val Ser Gln Leu Lys Arg Tyr Val Glu
Tyr Phe Lys Ser Lys Tyr Gly 165 170 175Arg Asp Arg Val Arg Gly Ile
Leu Val Ala Pro Ser Leu Thr Val Gly 180 185 190Ala Glu Arg Leu Leu
Lys Glu Glu Asn Leu Glu Phe Lys Lys Leu Asn 195 200 205Pro Pro Lys
Gly Ser Lys Lys Asp Leu Lys Gln Asn Met Lys Ser 210 215
22093221PRTThermococcus 93Val Asn Ile Phe Ala His Cys Arg Val Phe
Tyr Asp Gly Arg Ala Lys1 5 10 15Ser Glu Leu Gly Pro Gly Asp Arg Val
Ile Leu Ile Lys Pro Asp Gly 20 25 30Ser Phe Leu Ile His Gln Lys Glu
Lys Arg Glu Pro Val Asn Trp Gln 35 40 45Pro Pro Gly Ser Ser Val Gly
Leu Glu Ile Asn Asp Gly Lys Leu Phe 50 55 60Leu Arg Ser Val Arg Arg
Lys Pro Arg Glu Ile Leu Glu Val Glu Leu65 70 75 80Leu Asn Val Tyr
Leu Ile Ser Tyr Phe Gln Ala Glu Asp Tyr Glu Glu 85 90 95Leu Ala Leu
Thr Gly Ser Glu Ala Glu Met Ala Asp Leu Ile Phe Glu 100 105 110Asp
Pro Ser Leu Ile Glu Ala Gly Phe Lys Pro Leu Phe Arg Glu Lys 115 120
125Pro Ile Lys His Gly Ile Val Asp Val Leu Gly Val Asp Lys Glu Gly
130 135 140Asn Ile Val Ile Leu Glu Leu Lys Arg Arg Arg Ala Asp Leu
His Ala145 150 155 160Val Ser Gln Leu Lys Arg Tyr Val Glu Ala Met
Arg Glu Glu His Glu 165 170 175Lys Val Arg Gly Ile Leu Val Ala Pro
Ser Leu Thr Ser Gly Ala Lys 180 185 190Lys Leu Leu Glu Lys Glu Gly
Leu Glu Phe Arg Lys Leu Thr Pro Pro 195 200 205Lys Arg Gly Lys Ser
Lys Arg Gly Arg Gln Lys Thr Leu 210 215 22094215PRTThermococcus
sibiricus 94Val Thr Ile Phe Ala Arg Cys Asn Val Ser Tyr Asp Gly Arg
Ala Lys1 5 10 15Ser Glu Leu Gly Leu Gly Asp Arg Val Ile Ile Ile Lys
Pro Asp Gly 20 25 30Ser Phe Leu Ile His Gln Lys Glu Lys Arg Glu Pro
Val Asn Trp Gln 35 40 45Pro Pro Gly Ser Ser Val Ser Leu Lys Ile Gly
Glu Asp Lys Leu Ile 50 55 60Leu Arg Ser Val Arg Arg Lys Pro Lys Glu
Ile Leu Glu Val Gly Leu65 70 75 80Ile Asp Val Tyr Leu Leu Ser Tyr
Phe Gln Ala Glu Asp Tyr Glu Ser 85 90 95Leu Ala Leu Met Gly Ser Glu
Ala Glu Met Ala Asp Leu Val Phe Glu 100 105 110Asn Pro Glu Leu Ile
Glu Pro Gly Phe Lys Pro Ile Phe Lys Glu Lys 115 120 125Ser Ile Arg
His Gly Ile Ile Asp Ile Leu Gly Lys Asp Lys Asp Gly 130 135 140Asn
Ile Val Val Leu Glu Phe Lys Arg Arg Arg Ala Asp Leu His Ala145 150
155 160Val Ser Gln Leu Lys Arg Tyr Val Glu Thr Met Arg Glu Glu Tyr
Lys 165 170 175Asn Val Arg Gly Ile Leu Val Ala Pro Ser Leu Thr Ser
Gly Ala Lys 180 185 190Arg Leu Leu Glu Lys Glu Gly Leu Glu Phe Lys
Lys Leu Thr Pro Pro 195 200 205Lys Lys Glu Lys Ser Arg Lys 210
21595221PRTThermococcus piezophilus 95Leu Thr Ile Phe Ala Arg Cys
Lys Val His Tyr Asp Gly Arg Ala Lys1 5 10 15Ser Glu Leu Gly Ser Gly
Asp Arg Val Ile Leu Val Lys Pro Asp Gly 20 25 30Ala Phe Leu Ile His
Gln Ser Lys Lys Arg Glu Pro Val Asn Trp Gln 35 40 45Pro Pro Gly Ser
Phe Val Thr Val Glu Glu Arg Asp Gly Ile Ile Val 50 55 60Leu Arg Ser
Val Arg Arg Lys Pro Lys Glu Ile Leu Glu Val Glu Leu65 70 75 80Glu
Glu Val Tyr Leu Ala Ser Leu Phe Lys Ala Glu Asp Tyr Glu Glu 85 90
95Leu Ala Leu Thr Gly Ser Glu Ala Glu Met Ala Glu Met Ile Phe Lys
100 105 110Asn Pro Glu Leu Ile Glu Pro Gly Phe Arg Pro Leu Phe Arg
Glu Lys 115 120 125Ser Ile Gly His Gly Ile Val Asp Ile Leu Gly Arg
Asp Arg Glu Gly 130 135 140Asn Leu Val Val Leu Glu Leu Lys Arg Arg
Arg Ala Asp Leu His Ala145 150 155 160Val Ser Gln Leu Lys Arg Tyr
Val Glu Ala Leu Arg Ala Glu His Pro 165 170 175Ala Val Arg Gly Ile
Leu Val Ala Pro Ser Leu Thr Ser Gly Ala Lys 180 185 190Lys Leu Leu
Glu Lys Glu Gly Leu Glu Phe Arg Arg Val Gln Pro Pro 195 200 205Lys
Arg Glu Ser Val Ala Lys Gly Arg Gln Thr Thr Leu 210 215
22096221PRTThermococcus 96Val Asn Ile Phe Ala His Cys Arg Val Phe
Tyr Asp Gly Arg Ala Lys1 5 10 15Ser Glu Leu Gly Pro Gly Asp Arg Val
Ile Leu Ile Lys Pro Asp Gly 20 25 30Ser Phe Leu Ile His Gln Lys Glu
Lys Arg Glu Pro Val Asn Trp Gln 35 40 45Pro Pro Gly Ser Ser Val Gly
Leu Glu Val Lys Glu Gly Arg Ile Phe 50 55 60Leu Arg Ser Ile Arg Arg
Lys Pro Arg Glu Ile Leu Glu Val Glu Leu65 70 75 80Leu His Val Tyr
Leu Ile Ser Tyr Phe Gln Ala Glu Asp Tyr Glu Glu 85 90 95Leu Ala Leu
Thr Gly Ser Glu Ala Glu Met Ala Asp Leu Ile Phe Glu 100 105 110Asn
Pro Ser Val Ile Glu Asp Gly Phe Lys Pro Leu Phe Lys Glu Lys 115 120
125Pro Ile Lys His Gly Ile Val Asp Val Leu Gly Val Asp Lys Glu Gly
130 135 140Asn Ile Val Val Leu Glu Leu Lys Arg Arg Arg Ala Asp Leu
His Ala145 150 155 160Val Ser Gln Leu Lys Arg Tyr Val Glu Ala Met
Lys Glu Glu His Glu 165 170 175Lys Val Arg Gly Ile Leu Val Ala Pro
Ser Leu Thr Ser Gly Ala Lys 180 185 190Lys Leu Leu Glu Lys Glu Gly
Leu Glu Phe Arg Arg Leu Thr Pro Pro 195 200 205Lys Arg Gly Lys Ser
Lys Arg Gly Arg Gln Lys Thr Leu 210 215 22097221PRTThermococcus
thioreducens 97Leu Thr Ile Phe Ala Arg Cys Lys Val His Tyr Asp Gly
Arg Ala Lys1 5 10 15Ser Glu Leu Gly Ser Gly Asp Arg Val Ile Ile Ile
Lys Pro Asp Gly 20 25 30Ala Phe Leu Ile His Gln Ser Arg Lys Arg Glu
Pro Val Asn Trp Gln 35 40 45Pro Pro Gly Ser Phe Val Met Met Glu Glu
Arg Asp Gly Ile Leu Val 50 55 60Leu Arg Ser Val Arg Arg Lys Pro Lys
Glu Ile Leu Glu Val Glu Leu65 70 75 80Glu Glu Val Tyr Leu Ile Ser
Leu Phe Lys Ala Glu Asp Tyr Glu Glu 85 90 95Leu Ala Leu Thr Gly Ser
Glu Ala Glu Met Ala Glu Met Val Phe Arg 100 105 110Asn Pro Glu Leu
Ile Glu Pro Gly Phe Lys Pro Leu Phe Arg Glu Lys 115 120 125Gln Ile
Gly His Gly Ile Val Asp Ile Leu Gly Arg Asp Arg Asp Gly 130 135
140Asn Leu Val Val Leu Glu Leu Lys Arg Arg Lys Ala Asp Leu His
Ala145 150 155 160Val Ser Gln Leu Lys Arg Tyr Val Glu Ala Leu Lys
Arg Glu His Glu 165 170 175Thr Val Arg Gly Ile Leu Val Ala Pro Ser
Leu Thr Ala Gly Ala Lys 180 185 190Lys Leu Leu Glu Lys Glu Gly Leu
Glu Phe Arg Arg Val Gln Pro Pro 195 200 205Lys Arg Glu Lys Phe Gly
Arg Gly Arg Gln Lys Thr Leu 210 215 22098221PRTThermococcus
litoralis 98Val Asn Ile Phe Ala His Cys Arg Val Phe Tyr Asp Gly Arg
Ala Lys1 5 10 15Ser Glu Leu Gly Pro Gly Asp Arg Val Ile Leu Ile Lys
Pro Asp Gly 20 25 30Ser Phe Leu Ile His Gln Lys Glu Lys Arg Glu Pro
Val Asn Trp Gln 35 40 45Pro Pro Gly Ser Ser Val Gly Leu Glu Val Lys
Glu Asp Lys Ile Phe 50 55 60Leu Arg Ser Ile Arg Arg Lys Pro Arg Glu
Ile Leu Glu Val Glu Leu65 70 75 80Leu Asn Val Tyr Leu Ile Ser Tyr
Phe Gln Ala Glu Asp Tyr Glu Glu 85 90 95Leu Ala Leu Thr Gly Ser Glu
Ala Glu Met Ala Asp Leu Ile Phe Glu 100 105 110Asn Pro Ser Leu Ile
Glu Asp Gly Phe Lys Pro Leu Phe Lys Glu Lys 115 120 125Pro Ile Lys
His Gly Ile Val Asp Val Leu Gly Val Asp Lys Glu Gly 130 135 140Asn
Ile Val Ile Leu Glu Leu Lys Arg Arg Arg Ala Asp Leu His Ala145 150
155 160Val Ser Gln Leu Lys Arg Tyr Val Glu Ala Met Arg Glu Glu His
Glu 165 170 175Lys Val Arg Gly Ile Leu Val Ala Pro Ser Leu Thr Ser
Gly Ala Lys 180 185 190Lys Leu Leu Glu Lys Glu Gly Leu Glu Phe Arg
Lys Leu Thr Pro Pro 195 200 205Lys Arg Gly Lys Ser Lys Arg Gly Arg
Gln Lys Thr Leu 210 215 22099221PRTThermococcus onnurineus 99Leu
Thr Ile Phe Ala Arg Cys Lys Val His Tyr Asp Gly Arg Ala Lys1 5 10
15Ser Glu Leu Gly Ser Gly Asp Arg Val Ile Leu Val Lys Pro Asp Gly
20 25 30Ala Phe Leu Ile His Gln Ser Lys Lys Arg Glu Pro Val Asn Trp
Gln 35 40 45Pro Pro Gly Ser Phe Val Thr Val Glu Glu Arg Asp Gly Ile
Ile Val 50 55 60Leu Arg Ser Val Arg Arg Lys Pro Lys Glu Ile Leu Glu
Val Glu Leu65 70 75 80Glu Glu Val Tyr Leu Ala Ser Leu Phe Lys Ala
Glu Asp Tyr Glu Glu 85 90 95Leu Ala Leu Thr Gly Ser Glu Ala Glu Met
Ala Glu Met Ile Phe Lys 100 105 110Asn Pro Glu Leu Ile Glu Pro Gly
Phe Arg Pro Leu Phe Arg Glu Lys 115 120 125Ser Ile Gly His Gly Ile
Val Asp Ile Leu Gly Arg Asp Arg Glu Gly 130 135 140Asn Leu Val Val
Leu Glu Leu Lys Arg Arg Arg Ala Asp Leu His Ala145 150 155 160Val
Ser Gln Leu Lys Arg Tyr Val Glu Ala Leu Arg Ala Glu His Pro 165 170
175Ala Val Arg Gly Ile Leu Val Ala Pro Ser Leu Thr Ser Gly Ala Lys
180 185 190Lys Leu Leu Glu Lys Glu Gly Leu Glu Phe Arg Arg Val Gln
Pro Pro 195 200 205Lys Arg Glu Ser Val Thr Lys Gly Arg Gln Thr Thr
Leu 210 215 220100215PRTThermococcus celericrescens 100Leu Thr Ile
Phe Ala Arg Cys Lys Val His Tyr Asp Gly Arg Ala Lys1 5 10 15Ser Glu
Leu Gly Ser Gly Asp Arg Val Ile Ile Ile Lys Pro Asp Gly 20 25 30Ala
Phe Leu Ile His Gln Ser Lys Lys Arg Glu Pro Val Asn Trp Gln 35 40
45Pro Pro Gly Ser Phe Val Thr Val Glu Glu Arg Asp Gly Ile Ile Ile
50 55 60Leu Arg Ser Val Arg Arg Lys Pro Lys Glu Ile Leu Glu Val Glu
Leu65 70 75 80Glu Glu Val Tyr Leu Val Ser Leu Phe Lys Ala Glu Asp
Tyr Glu Glu 85 90 95Leu Thr Leu Thr Gly Ser Glu Ala Glu Met Ala Glu
Met Ile Phe Gly 100 105 110Asn Pro Glu Leu Ile Glu Pro Gly Phe Lys
Pro Leu Phe Arg Glu Lys 115 120 125Gln Ile Gly His Gly Ile Val Asp
Ile Leu Gly Arg Asp Lys Asn Gly 130 135 140Asn Leu Val Val Leu Glu
Leu Lys Arg Arg Lys Ala Asp Leu His Ala145 150 155 160Val Ser Gln
Leu Lys Arg Tyr Val Glu Gly Leu Ser Lys Glu His Glu 165 170 175Gly
Val Arg Gly Ile Leu Val Ala Pro Ser Leu Thr Ser Gly Ala Lys 180
185
190Arg Leu Leu Glu Lys Glu Gly Leu Glu Phe Arg Lys Val Gln Pro Pro
195 200 205Lys Arg Glu Lys Leu Gly Lys 210 215101215PRTThermococcus
101Val Thr Ile Phe Ala His Cys Lys Val Phe Tyr Asp Gly Arg Ala Lys1
5 10 15Ser Glu Leu Gly Pro Gly Asp Arg Val Ile Ile Ile Lys Pro Asp
Gly 20 25 30Ser Phe Leu Ile His Gln Lys Glu Lys Arg Glu Pro Val Asn
Trp Gln 35 40 45Pro Pro Gly Ser Ser Val Ser Leu Asp Val Lys Glu Asp
Lys Leu Ile 50 55 60Leu Arg Ser Val Arg Arg Lys Pro Lys Glu Ile Leu
Glu Val Glu Leu65 70 75 80Ile Asp Val Tyr Leu Phe Ser Tyr Phe Gln
Ala Glu Asp Tyr Glu Ala 85 90 95Leu Ala Leu Val Gly Ser Glu Ala Glu
Met Ala Asp Leu Val Phe Glu 100 105 110Asn Pro Glu Leu Ile Glu Asp
Gly Phe Lys Pro Leu Phe Lys Glu Lys 115 120 125Ser Ile Arg His Gly
Ile Val Asp Leu Leu Gly Lys Asp Lys Asp Gly 130 135 140Asn Ile Val
Ile Leu Glu Phe Lys Arg Arg Arg Ala Asp Leu His Ala145 150 155
160Val Ser Gln Leu Lys Arg Tyr Val Glu Thr Met Arg Glu Glu Tyr Glu
165 170 175Lys Val Arg Gly Ile Leu Val Ala Pro Ser Leu Thr Ser Gly
Ala Arg 180 185 190Arg Leu Leu Glu Lys Glu Gly Leu Glu Phe Lys Lys
Leu Lys Pro Pro 195 200 205Lys Gln Glu Lys Ser Arg Lys 210
215102215PRTThermococcus 102Leu Thr Ile Phe Ala Arg Cys Arg Val His
Tyr Asp Gly Arg Ala Lys1 5 10 15Ser Glu Leu Gly Ser Gly Asp Arg Val
Ile Ile Ile Lys Pro Asp Gly 20 25 30Ala Phe Leu Ile His Gln Ser Lys
Lys Arg Glu Pro Val Asn Trp Gln 35 40 45Pro Pro Gly Ser Phe Val Thr
Ile Glu Glu Arg Asp Gly Ile Ile Ile 50 55 60Leu Arg Ser Val Arg Arg
Lys Pro Lys Glu Ile Leu Glu Val Glu Leu65 70 75 80Glu Glu Val Tyr
Leu Val Ser Leu Phe Lys Ala Glu Asp Tyr Glu Glu 85 90 95Leu Ala Leu
Thr Gly Ser Glu Ala Glu Met Ala Glu Met Ile Phe Arg 100 105 110Asn
Pro Glu Leu Ile Glu Pro Gly Phe Lys Pro Leu Phe Arg Glu Lys 115 120
125Gln Ile Gly His Gly Ile Val Asp Ile Leu Gly Arg Asp Gly Asp Gly
130 135 140Asn Leu Val Val Leu Glu Leu Lys Arg Arg Lys Ala Asp Leu
His Ala145 150 155 160Val Ser Gln Leu Lys Arg Tyr Val Glu Ala Leu
Ser Arg Glu His Glu 165 170 175Ser Val Arg Gly Ile Leu Val Ala Pro
Ser Leu Thr Ser Gly Ala Lys 180 185 190Lys Leu Leu Glu Lys Glu Gly
Leu Glu Phe Arg Lys Val Gln Pro Pro 195 200 205Lys Arg Glu Lys Leu
Gly Lys 210 215103213PRTThermococcus kodakarensis 103Leu Thr Ile
Phe Ala Arg Cys Lys Val His Tyr Asp Gly Arg Ala Lys1 5 10 15Ser Glu
Leu Gly Ser Gly Asp Arg Val Ile Ile Val Lys Pro Asp Gly 20 25 30Ser
Phe Leu Ile His Gln Ser Lys Lys Arg Glu Pro Val Asn Trp Gln 35 40
45Pro Pro Gly Ser Arg Val Arg Leu Glu Leu Arg Glu Asn Pro Val Leu
50 55 60Val Ser Ile Arg Arg Lys Pro Arg Glu Thr Leu Glu Val Glu Leu
Glu65 70 75 80Glu Val Tyr Met Val Ser Val Phe Arg Ala Glu Asp Tyr
Glu Glu Leu 85 90 95Ala Leu Thr Gly Ser Glu Ala Glu Met Ala Glu Leu
Ile Phe Glu Asn 100 105 110Pro Glu Val Ile Glu Pro Gly Phe Lys Pro
Leu Phe Arg Glu Lys Ala 115 120 125Ile Gly Thr Gly Ile Val Asp Val
Leu Gly Arg Asp Ser Asp Gly Asn 130 135 140Ile Val Val Leu Glu Leu
Lys Arg Arg Arg Ala Glu Leu His Ala Val145 150 155 160Arg Gln Leu
Lys Ser Tyr Val Glu Ile Leu Arg Glu Glu Tyr Gly Asp 165 170 175Lys
Val Arg Gly Ile Leu Val Ala Pro Ser Leu Thr Ser Gly Ala Lys 180 185
190Arg Leu Leu Glu Lys Glu Gly Leu Glu Phe Arg Lys Leu Glu Pro Pro
195 200 205Lys Arg Asp Ser Lys 210104208PRTThermococcus
peptonophilus 104Leu Thr Ile Phe Ala Arg Cys Lys Val His Tyr Asp
Gly Arg Ala Lys1 5 10 15Ser Glu Leu Gly Ser Gly Asp Arg Val Ile Ile
Val Lys Pro Asp Gly 20 25 30Ser Phe Leu Ile His Gln Ser Lys Lys Arg
Glu Pro Val Asn Trp Gln 35 40 45Pro Pro Gly Ser Arg Val Arg Leu Glu
Leu Arg Glu Asn Pro Val Leu 50 55 60Val Ser Ile Arg Arg Lys Pro Lys
Glu Thr Leu Glu Val Glu Leu Glu65 70 75 80Glu Val Tyr Met Val Ser
Val Phe Arg Ala Glu Asp Tyr Glu Glu Leu 85 90 95Thr Leu Thr Gly Ser
Glu Ala Glu Met Ala Glu Leu Ile Phe Glu Asn 100 105 110Pro Glu Val
Ile Glu Pro Gly Phe Lys Pro Leu Phe Arg Glu Lys Thr 115 120 125Ile
Lys Ser Gly Ile Val Asp Ile Leu Gly Arg Asp Ser Asn Gly Asn 130 135
140Ile Val Val Leu Glu Leu Lys Arg Arg Arg Ala Glu Leu His Ala
Val145 150 155 160Arg Gln Leu Lys Ser Tyr Val Glu Ile Leu Lys Glu
Glu Tyr Gly Asp 165 170 175Lys Val Arg Gly Ile Leu Val Ala Pro Ser
Leu Thr Ser Gly Ala Lys 180 185 190Arg Leu Leu Glu Lys Glu Gly Leu
Glu Phe Arg Lys Leu Glu Pro Pro 195 200 20510524DNAArtificial
SequenceSynthetic consstruct 105cgccagggtt ttcccagtca cgac
2410624DNAArtificial SequenceSynthetic construct 106gtcgtgactg
ggaaaaccct ggcg 2410724DNAArtificial SequenceSynthetic construct
107gtcgtgactg ggtaaaccct ggcg 2410876DNAArtificial
SequenceSynthetic constuct 108atctgatcgg aagagcacac gtctgaactc
cagtctacac tctttcccta cacgacgctc 60ttccgatctg atcgga
7610954DNAArtificial SequenceSynthetic construct 109agagcacacg
tctgaactcc agtctacact ctttccctac acgacgctct tccg 54
uspto.report is an independent third-party trademark research tool that is not affiliated, endorsed, or sponsored by the United States Patent and Trademark Office (USPTO) or any other governmental organization. The information provided by uspto.report is based on publicly available data at the time of writing and is intended for informational purposes only.
While we strive to provide accurate and up-to-date information, we do not guarantee the accuracy, completeness, reliability, or suitability of the information displayed on this site. The use of this site is at your own risk. Any reliance you place on such information is therefore strictly at your own risk.
All official trademark data, including owner information, should be verified by visiting the official USPTO website at www.uspto.gov. This site is not intended to replace professional legal advice and should not be used as a substitute for consulting with a legal professional who is knowledgeable about trademark law.