U.S. patent application number 17/577460 was filed with the patent office on 2022-06-23 for bee nutrition.
This patent application is currently assigned to APIX BIOSCIENCES BIOSCIENCES NV aka APIX BIOSCIENCES. The applicant listed for this patent is APIX BIOSCIENCES BIOSCIENCES NV aka APIX BIOSCIENC. Invention is credited to Sue NICOLSON, Sharoni SHAFIR, Phil STEVENSON, Geraldine WRIGHT.
Application Number | 20220192232 17/577460 |
Document ID | / |
Family ID | |
Filed Date | 2022-06-23 |
United States Patent
Application |
20220192232 |
Kind Code |
A1 |
WRIGHT; Geraldine ; et
al. |
June 23, 2022 |
BEE NUTRITION
Abstract
A method for increasing bee performance by administering pollen
substitutes to bees where the pollen substitute includes sources of
proteins, fatty acids and carbohydrates.
Inventors: |
WRIGHT; Geraldine;
(Newcastle Upon Tyne, GB) ; SHAFIR; Sharoni;
(Jerusalem, IL) ; NICOLSON; Sue; (Pretoria,
ZA) ; STEVENSON; Phil; (London, GB) |
|
Applicant: |
Name |
City |
State |
Country |
Type |
APIX BIOSCIENCES BIOSCIENCES NV aka APIX BIOSCIENC |
Wingene |
|
BE |
|
|
Assignee: |
APIX BIOSCIENCES BIOSCIENCES NV aka
APIX BIOSCIENCES
Wingene
BE
|
Appl. No.: |
17/577460 |
Filed: |
January 18, 2022 |
Related U.S. Patent Documents
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Application
Number |
Filing Date |
Patent Number |
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15776371 |
May 15, 2018 |
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PCT/GB2016/053573 |
Nov 16, 2016 |
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17577460 |
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International
Class: |
A23K 50/90 20060101
A23K050/90; A23K 20/105 20060101 A23K020/105; A23K 20/158 20060101
A23K020/158; A23K 20/163 20060101 A23K020/163; A23K 20/20 20060101
A23K020/20; A23K 20/147 20060101 A23K020/147; A23K 20/168 20060101
A23K020/168; A23K 20/174 20060101 A23K020/174; A23K 20/111 20060101
A23K020/111 |
Foreign Application Data
Date |
Code |
Application Number |
Nov 16, 2015 |
GB |
1520144.5 |
Claims
1-59. (Canceled)
60. A method for increasing bee performance, comprising:
administering to a bee a pollen substitute composition, comprising:
a) at least one component which is a source of protein; b) at least
one component which is a source of one or more fatty acids; and c)
at least one component which is a source of one or more
carbohydrates; wherein: the ratio of protein and one or more fatty
acids in the composition is between about 1 (protein):10 (fatty
acid) w/w and about 20 (protein):1 (fatty acid) w/w; and the
composition comprises linoleic acid (LA) and .alpha.-linolenic acid
(ALA) in a ratio of between about 5:1 (LA:ALA) to 1:20 (LA:ALA) as
compared to a composition comprising linoleic acid (LA) and
.alpha.-linolenic acid (ALA) in a ratio of more than 5:1 (LA:ALA)
or less than 1:20 (LA:ALA).
61. The method of claim 60, wherein administration of the
composition increases palatability as compared to a composition
comprising linoleic acid (LA) and .alpha.-linolenic acid (ALA) in a
ratio of more than 5:1 (LA:ALA) or less than 1:20 (LA:ALA).
62. The method of claim 60, wherein administration of the
composition increases brood production as compared to a composition
comprising linoleic acid (LA) and .alpha.-linolenic acid (ALA) in a
ratio of more than 5:1 (LA:ALA) or less than 1:20 (LA:ALA).
63. The method of claim 60, wherein administration of the
composition increases foraging as compared to a composition
comprising linoleic acid (LA) and .alpha.-linolenic acid (ALA) in a
ratio of more than 5:1 (LA:ALA) or less than 1:20 (LA:ALA).
64. The method of claim 60, wherein administration of the
composition increases cognitive function as compared to a
composition comprising linoleic acid (LA) and .alpha.-linolenic
acid (ALA) in a ratio of more than 5:1 (LA:ALA) or less than 1:20
(LA:ALA).
65. The method of claim 60, wherein administration of the
composition increases consumption as compared to a composition
comprising linoleic acid (LA) and .alpha.-linolenic acid (ALA) in a
ratio of more than 5:1 (LA:ALA) or less than 1:20 (LA:ALA).
66. The method of claim 60, wherein administration of the
composition increases survival as compared to a composition
comprising linoleic acid (LA) and .alpha.-linolenic acid (ALA) in a
ratio of more than 5:1 (LA:ALA) or less than 1:20 (LA:ALA).
67. The method of claim 60, wherein administration of the
composition increases bee size as compared to a composition
comprising linoleic acid (LA) and .alpha.-linolenic acid (ALA) in a
ratio of more than 5:1 (LA:ALA) or less than 1:20 (LA:ALA).
68. The method of claim 60, wherein administration of the
composition increases honey production as compared to a composition
comprising linoleic acid (LA) and .alpha.-linolenic acid (ALA) in a
ratio of more than 5:1 (LA:ALA) or less than 1:20 (LA:ALA).
69. The method of claim 60, wherein administration of the
composition increases biological fitness as compared to a
composition comprising linoleic acid (LA) and .alpha.-linolenic
acid (ALA) in a ratio of more than 5:1 (LA:ALA) or less than 1:20
(LA:ALA).
Description
CROSS-REFERENCE TO RELATED APPLICATIONS
[0001] This application is a division of U.S. patent application
Ser. No. 15/776,371 filed on May 15, 2018, as the U.S. National
Stage of International Patent Application No. PCT/GB2016/053573,
filed on Nov. 16, 2016, which claims the benefit of Great Britain
Patent Application No. 1520144.5 filed Nov. 16, 2015, the
disclosures of each of which are expressly incorporated by
reference in their entirety.
FIELD OF THE INVENTION
[0002] The present invention relates to compositions, methods and
products for providing nutrition to insects such as bees, e.g.,
honey bees. Certain embodiments of the present invention also
relate to methods of providing nutrition to bees e.g., colonies of
bees together with methods of preparing a composition for providing
nutrition to bees.
BACKGROUND TO THE INVENTION
[0003] Domesticated honeybees are important for world agriculture
as pollinators of crops. They are also kept for the production of
honey, wax, and royal jelly for human use. Commercial beekeeping
operations rear millions of colonies every year for transport to
areas of intense cultivation of soft fruit and nut crops throughout
the world. Commercially-reared bumblebee colonies have been in
production for the past 30 years. Bumblebees were domesticated to
meet the need for pollination services of glasshouse-grown crops
such as tomatoes that require pollination to set fruit. The demand
for pollination services has expanded with the need to provide food
for the growing world human population.
[0004] Historically, honeybee keepers have relied on natural forage
for bees provided by floral nectar and pollen to supply the
nutrition of their colonies. However, several factors have limited
their ability to feed large bee populations required for
pollination services. Of greatest impact has been the conversion of
natural landscapes with diverse flora to urban or agricultural use,
limiting the flowering plants available to feed bee populations.
Commercial honeybee keepers transport hives to orchards for
pollination services during winter, just prior to crop bloom and
other forms of spring forage. In these conditions (e.g., almond
orchards), bee colonies are in high density but have few natural
resources. Without pollen to feed brood, honeybee colonies struggle
to grow quickly enough to perform sufficient pollination services
to crops. Furthermore, crops are often monocultures. Monocultures
may provide pollen, but this pollen is unlikely to be a complete
source of essential nutrients needed for growth and maintenance of
honeybees.
[0005] Honeybees and other bee species collect pollen from flowers
to obtain protein, fats, sterols, vitamins, and minerals. They also
collect floral nectar, aphid honeydew, and other sugar sources like
tree sap to provide them with carbohydrates. Both adult and larval
stages depend on pollen and nectar for nourishment. Their
metabolism is biased very strongly towards the need for
carbohydrates and they also have unique needs for dietary sterols
which are not common to other insects outside of the
Hymenoptera.
[0006] Researchers have studied bee nutrition for over 100 years,
but no one has been able to replicate the nutrition provided by
multiple and diverse sources of floral pollen collected by
naturally foraging honeybees. The most common way to feed honeybees
during periods when they cannot obtain sufficient pollen is to add
pollen substitutes to the colony in the form of a patty or a
solution. These substitutes are typically composed of plant (e.g.
soy or corn gluten) and/or animal proteins (e.g. whole eggs), oils,
and brewer's yeast.
[0007] Most pollen supplement or substitute formulations are
unpalatable to bees and provide inadequate nutrition. For this
reason, existing supplements require the addition of floral,
bee-collected pollen to make them attractive and usable for long
periods of time. There are other problems with pollen substitutes
added to honeybee colonies. For example, these mixtures also
contain chemical compounds that are toxic or have negative impacts
on honeybee metabolism. In addition, many bee food formulations
contain proteins and other compounds that bees cannot digest.
Furthermore, some commercially available formulations contain very
high concentrations of protein which have been shown to reduce
worker bee lifespan. Indeed, the negative impact of feeding
honeybees with incorrectly formulated pollen substitutes in
commercial beekeeping operations has been argued as one of the
contributing factors to colony losses in the USA and elsewhere in
the world. These supplements may also cause poor foraging
performance and, therefore, inadequate pollination services in the
bees that survive.
[0008] Bumblebee colony producers exclusively use honeybee
collected pollen pellets as their source of pollen for colony
production. This makes the bumblebee production industry entirely
reliant on a food source collected by foraging honeybee colonies.
Problems with this include the potential exposure of bumblebees to
parasites and pathogens that infect honeybee colonies. It also
means that bumblebee producers have little control over the quality
of the food they provide.
[0009] It is an aim of aspects of the present invention to at least
partially mitigate the problems associated with the prior art.
[0010] It is an aim of certain embodiments of the present invention
to provide a composition which supplies insects e.g., honeybees,
with all or substantially all of the nutrients required for
survival, health, development and best foraging performance.
[0011] It is an aim of certain embodiments of the present invention
to provide a composition which is a pollen supplement.
[0012] It is an aim of certain embodiments of the present invention
to provide a composition which is a pollen substitute.
SUMMARY OF CERTAIN EMBODIMENTS OF THE INVENTION
[0013] The composition of certain embodiments of the present
invention includes a mixture of macronutrients and micronutrients
as found in naturally-occurring floral pollen that provides the
correct balance of nutrients for honeybees. In certain embodiments,
the pollen substitute provides a source of nutrition that does not
require the addition of floral pollen to make it palatable to bees,
to sustain brood production, and provide them with key nutrients
and their combinations that result in best foraging performance.
This combination of traits--palatability, brood production, and
ideal foraging and cognitive function--will hitherto be referred to
as `improved bee performance.`
[0014] In certain embodiments, the present invention provides a
pollen substitute for use in honeybee colonies as a replacement for
floral pollen.
[0015] In certain embodiments, the present invention provides a
pollen supplement for use in honeybee colonies as an additive to
floral pollen.
[0016] Certain embodiments of the invention specifically define the
ratios of macronutrients and micronutrients that enable improved
bee performance. Aptly, certain embodiments provide the proportions
of proteins (amino acids), carbohydrates, and fats required by
honeybees. Certain embodiments of the present invention also
provide the amounts of essential amino acids and non-essential
amino acids, carbohydrates, and fatty acids and the relative
proportions of these classes of macronutrients needed for optimal
performance in bee colonies.
[0017] In a first aspect of the present invention, there is
provided a composition for providing nutrition to bees, the
composition comprising: [0018] a) at least one component which is a
source of protein (P); [0019] b) at least one component which is a
source of one or more fatty acids (FA); and [0020] c) at least one
component which is a source of one or more carbohydrates (C),
wherein the ratio of the source of protein and the source of one or
more fatty acids in the composition is between about 1 (protein):10
(fatty acid) w/w and about 20 (protein):1 (fatty acid) w/w.
[0021] In certain embodiments, the ratio of the source of protein
and the source of one or more fatty acids in the composition is
between about 1 (protein):5 (fatty acid) w/w and about 20
(protein):1 (fatty acid) w/w. In certain embodiments, the ratio of
the source of protein and the source of one or more fatty acids in
the composition is between about 1 (protein):1 (fatty acid) w/w and
about 20 (protein):1 (fatty acid) w/w.
[0022] In certain embodiments, the source of one or more fatty
acids may be a source of fat. The source of fat may be a single
component or a combination of components.
[0023] In certain embodiments, the composition comprises a
component or plurality of components which is/are a source of
essential fatty acids, linoleic acid (LA) and .alpha.-linolenic
acid (ALA). In certain embodiments, the composition comprises
linoleic acid and .alpha.-linolenic acid in a ratio of between
about 5:1 (linoleic acid:.alpha.-linolenic acid) to 1:10 (linoleic
acid:.alpha.-linolenic acid). Aptly, the composition comprises a
low ratio of linoleic acid:.alpha.-linolenic acid. In certain
embodiments, the ratio about 3:1 (linoleic acid:.alpha.-linolenic
acid) to 1:3 (linoleic acid:.alpha.-linolenic acid). Aptly, the
composition comprises a low ratio of linoleic
acid:.alpha.-linolenic acid.
[0024] In certain embodiments, the ratio of the component which is
a source of protein and the component which is a source of
carbohydrate is between about 2 (protein):1 (carbohydrate) and
about 1 (protein):10 (carbohydrate).
[0025] Further details of the composition are provided below.
[0026] In a second aspect of the present invention, there is
provided a product for providing nutrition to bees comprising a
composition according to the first aspect of the present invention,
wherein the product is solid. Aptly, the composition is: [0027] i)
moist; [0028] ii) baked; and/or [0029] iii) powdered.
[0030] In a third aspect of the present invention, there is
provided a product for providing nutrition to bees comprising a
composition according to the first aspect of the present invention,
wherein the product is a liquid.
[0031] In a fourth aspect of the present invention, there is
provided a method of providing nutrition to a bee comprising:
[0032] a) providing a composition according to the first aspect of
the present invention or a product according to the second and/or
third aspects of the present invention to a bee, wherein the bee is
comprised in a colony of bees.
[0033] In a fifth aspect of the present invention, there is
provided a method of reducing bee mortality comprising; [0034] a)
providing a composition according to the first aspect of the
present invention or a product according to the second and/or third
aspects of the present invention to a bee, wherein the bee is
comprised in a colony of bees.
BRIEF DESCRIPTION OF THE FIGURES
[0035] Embodiments of the present invention will now be described
hereinafter, by way of example only, with reference to the
following figures:
[0036] FIG. 1 is a graph showing the learning performance results
of honeybees fed one of four diets.
[0037] FIG. 2 is a graph showing that biscuit size can affect the
amount of the food consumed by honeybees within hives. Small,
medium, and large pollen supplement patties had dimensions of small
(100.times.100.times.23 mm), medium (141.times.141.times.11.5 mm)
and large (200.times.200.times.5.8 mm), respectively. Food
consumption of the patties was measured on a weekly basis over 6
weeks. Of the pollen supplement patties, the large surface area
patty was consumed most. Candy refers to a formulation of sucrose
only.
[0038] FIG. 3 comprising FIGS. 3a, 3b, and 3c, are graphs showing
that the proportion of protein-to-fat (P:FA) in diet affects
survival and food consumption of adult bees. In FIG. 3a, individual
adult worker bumblebees were fed with a diet composed of
carbohydrate (sucrose), protein (sodium caseinate), and fat
(lecithin) for 7 days. Fewer bees survived on diets with a high
proportion of fat; the optimal proportion (in solid red) was 10:1
(P:FA). N=15 per diet.
[0039] In FIG. 3b, adult worker honeybees (0-10 days old)
self-select diets that are 1:2.8 protein-to-fat when the diets are
composed of free amino acids and lecithin and the
protein-to-carbohydrate ratio is 1:25. The diets were composed of
protein in the form of free amino acids and fat in the form of
lecithin. N=15 cohorts of 30 bees per diet.
[0040] FIG. 3c shows that adult worker honeybees (0-10 days old)
survive well on diets that range over 1:0-1:5 parts protein-to-fat,
but have lower survival when the diets are biased towards high fat
(1:10 or greater). Particularly, newly-emerged adult worker
honeybees survive well on diets proportionally high in fat, as long
as the amount of fat is .gtoreq.1:10 protein-to-fat. N=15 cohorts
of 30 bees per diet.
[0041] FIG. 4 is a graph showing the learning performance results
of honeybees fed one of 12 diets. Newly emerged bees were kept in
groups of five in a petri dish with ad lib access to three feeders
containing one of the diets, honey, and water. The amount of flax
and corn oil in the diets was varied so that the diets contained
between 0.8 and 38 mg/g omega-3 (ALA) and between 4 and 33 mg/g
omega-6 (LA). The total fatty acid proportion of the diet was
either 1, 2, 4, or 8%, and the omega-6:3 ratio was either 0.33, 1,
or 5. After one week bees were tested in an olfactory proboscis
extension response discrimination conditioning assay. The learning
index (delta PER) is the difference between the number of responses
to the odor associated with an appetitive (sugar) reward minus the
number of responses to the odor associated with an aversive (salt)
reward during the last three of six conditioning trials of every
subject. The x-axis shows the composition of each diet in terms of
omega-6:3 ratio, omega-3 amount (mg FA per g diet), and total fatty
acid (TFA) percent of the diet. Better performance was achieved
with omega-6:3 ratio of 1 or less, and TFA % of 2 or above, with
the optimum being TFA% of 4 and omega-6:3 ratio of 1. These data
support the importance of sufficient omega-3 in the diet, and the
deleterious effect of too much omega-6.
[0042] FIG. 5 is a graph that shows the ratio of essential amino
acids found in diet affects the protein-to-carbohydrate ratio
self-selected by cohorts of adult worker honeybees. Bees fed with
diets containing only one protein that does not contain the correct
proportions of essential amino acids will consume a diet
proportionally higher in protein to meet their nutritional
needs.
[0043] FIG. 6, comprising FIGS. 6A to 6H are graphs illustrating
the effect of various minerals and their concentration in the diet
of bees on adult worker honeybee survival. Minerals, metals and
salts in food are essential nutrients and affect bee survival but
they must be placed in food at concentrations that do not kill
bees. The dashed line represents the control diet treatment and
coloured (paler) line the highest mineral salt concentration
tested. Each concentration (in ppm) was tested on N=15 cohorts of
25 bees.
DETAILED DESCRIPTION OF EMBODIMENTS OF THE INVENTION
[0044] FIG. 1 is a graph showing the learning performance results
of honeybees fed one of four diets Two diets had a high omega-6:3
ratio and contained either corn or sesame oil. Bees fed on these
diets showed poor learning. Honeybees fed on one of two diets which
had a low omega-6:3 ratio, and which contained an oil mixture
comprising in addition to corn and sesame also sage and flax oils
or a pollen mixture showed good learning. Percent proboscis
extension (y-axis) describes the proportion of the bees in the
assay that exhibited conditioned olfactory learning.
[0045] Details of diets, showing percent of each essential fatty
acid of total fatty acids (TFA). The essential fatty acids comprise
about 50% or more of TFA (composition of non-essential fatty acids
not shown). Also shown is the omega-6:3 ratio.
TABLE-US-00001 TABLE 1 Treatment Diet/FA Corn Sesame Oil Mixture
Pollen Omega-6 53.85 46.08 41.82 21.41 (% of TFA) Omega-3 4.56 3.63
19.47 59.41 (% of TFA) Omega-6:3 11.81 12.69 2.15 0.36 ratio
[0046] Certain embodiments of the present invention relate to
compositions which have utility in providing nutrients to insects
such as, for example, bees.
[0047] The term `bee` refers to members of the Family Apidae, Order
Hymenoptera. A `honeybee` refers to members of the eusocial group
of bees that belong to the Genus Apis. For example, the
domesticated honeybee is Apis mellifera. Aptly, the composition of
certain embodiments of the present invention is for providing
nutrients to members of the Apis genus. A bumblebee refers to the
genus `Bombus`. For example, a domesticated species of bumblebee is
the buff-tailed bumblebee, Bombus terrestris.
[0048] The term `colony` refers to the collective group of eusocial
bees that belong to the same family unit, i.e. that arise from the
same queen. A `colony` is composed of a single queen (and also her
fertile daughters), sterile workers, brood (larvae/young bees), and
males (drones). This unit lives together on combs constructed in
the same location to rear brood that are the progeny of one queen.
Single honeybee colonies are housed in `hives` when domesticated.
Certain embodiments of the present invention provide a composition
for providing nutrition to a colony of bees e.g. a colony of
honeybees.
[0049] A colony of bees may be reared in a hive. As used herein,
the term `hive` refers to the wooden structure used by professional
beekeepers to rear a single colony of bees. Hives come in a variety
of forms throughout the world including but not limited to:
Langstroth hives, National Hives, WBC, Dadant, `Commercial`, Smith,
Warre, Top Bar, Rose OSB, Dartington, Centenary. They are generally
made of wood and characterized by removable frames upon which bees
can build wax comb for rearing bee brood and storing honey and
pollen.
[0050] As described herein, the composition of certain embodiments
of the present invention comprises a mixture of macronutrients and
micronutrients. Aptly, the composition provides a mixture of
components which provides a correct balance of nutrients for bees
e.g. honeybees. Aptly, the composition provides a pollen substitute
which does not require the addition of floral pollen, including for
example, bee collected pollen `pellets`, to make it palatable to
bees and to sustain brood production.
[0051] Unless otherwise specified, references to percentages are by
weight (wt %). Percentages of components in the composition of
certain embodiments of the invention are based on the total dry
weight of the composition.
[0052] In a first aspect of the present invention, there is
provided a composition for providing nutrition to bees, the
composition comprising: [0053] a) at least one component which is a
source of protein (P); [0054] b) at least one component which is a
source of one or more fatty acids (FA); and [0055] c) at least one
component which is a source of one or more carbohydrates (C)
wherein the ratio of the source of protein and the source of one or
more fatty acids in the composition is between about 1 (protein):10
(fatty acid) w/w and about 20 (protein):1 (fattyacid) w/w.
[0056] In certain embodiments, the composition comprises a
component or plurality of components which is/are a source of
essential fatty acids, linoleic acid and .alpha.-linolenic
acid.
[0057] In certain embodiments, the ratio of the source of protein
and the source of one or more fatty acids is between about 1
(protein):5 (fatty acid) w/w and about 20 (protein):1 (fatty acid)
w/w.
[0058] In certain embodiments, the ratio of the source of protein
and the source of one or more fatty acids is between about 1
(protein):1 (fatty acid) w/w and about 20 (protein):1 (fatty acid)
w/w.
[0059] In certain embodiments, the ratio of the component which is
a source of protein and the component which is a source of
carbohydrate is between about 2 (protein):1 (carbohydrate) and
about 1 (protein):10 (carbohydrate).
[0060] In certain embodiments, the ratio of protein:fats is between
about 25:1 and 1:10. Aptly, the ratio of protein:fats is between
about 1:5 and about 5:1.
[0061] In certain embodiments, the at least one component which is
a source of carbohydrate provided in a concentration of between
about 20% to about 90% by weight of the composition e.g. about 50%
to about 90% by dry weight of the composition.
[0062] In certain embodiments, the ratio of protein:carbohydrate is
between about 1:1 and about 1:5.
[0063] In certain embodiments, the composition comprises a high
omega 3 fatty acid:omega 6 fatty acid ratio. In some embodiments,
the composition comprises a ratio of omega 3 fatty acid:omega 6
fatty acid of about 1:1 to about 3:1. The fatty acids may be
provided by one or more components in the composition.
[0064] The components of the composition of certain embodiments are
described in more detail below:
[0065] In an embodiment, the composition comprises at least one
component which is a source of protein.
[0066] Aptly, the composition further comprises at least one
component which is a source of carbohydrate. Aptly, the composition
further comprises at least one component which is a source of a
lipid e.g. a fatty acid.
[0067] Thus, the composition provides macronutrients, e.g. protein,
lipid and carbohydrate, in predetermined ratios.
[0068] In certain embodiments, the at least one component which is
a source of protein is provided in a concentration of between about
10% and about 50% by total dry weight of the composition, e.g.
about 10% and about 30% by dry weight of the composition.
[0069] For example, the composition may comprise 10%, 15%, 20%,
25%, 30%, 35%, 40%, 45% or 50% protein by total dry weight of the
composition.
[0070] The component which is a source of protein may be selected
from one or more of the following: [0071] dehydrated egg white or
isolate thereof; [0072] fresh egg white or isolate thereof; [0073]
canola seed protein isolate; [0074] soy protein isolate; [0075]
hemp protein isolate; [0076] sodium and/or calcium caseinate;
[0077] bovine serum albumin; [0078] pea protein isolate; [0079]
rice protein isolate; [0080] spirulina protein isolate; [0081]
wheat gluten isolate; [0082] oat protein isolate; [0083] whey
protein isolate; [0084] fish meal; and [0085] blood meal (from
mammals); and [0086] dehydrated egg yolk protein or isolate
thereof.
[0087] Thus, in one embodiment, the composition comprises
dehydrated egg white or an isolate thereof. Aptly, the composition
comprises fresh egg white or an isolate thereof. In certain
embodiments, the composition comprises canola seed protein isolate.
Aptly, the canola seed protein isolate is Puratein.RTM. or
Supertein.RTM., both obtainable from Burcon Nutrascience. In one
embodiment, the canola seed protein isolate is obtained from a
commercial source such as for example Isolexx.RTM., a protein
product available from Teutexx proteins.
[0088] In certain embodiments, the composition comprises soy
protein isolate and/or hemp protein isolate. Aptly, the hemp
protein isolate is at least 70% protein (dry wt.)
[0089] Aptly, the soy protein isolate is ProFam 955.RTM. obtainable
from ADM Foods & Wellness, Illinois. In certain embodiments,
the oat protein isolate is PrOatein.RTM. obtainable from Tate &
Lyle, London.
[0090] In certain embodiments, the component which is a source of
protein is sodium caseinate or calcium caseinate. In an alternative
embodiment, the composition comprises bovine serum albumin. Aptly,
the composition comprises pea protein isolate. In certain
embodiments, the composition comprises rice protein isolate and/or
spirulina protein isolate and/or wheat gluten isolate.
[0091] In certain embodiments, dehydrated egg yolk may be obtained
from commercially available sources.
[0092] Aptly, the composition comprises a plurality of components,
each of which is a source of protein. It will be understood that if
the composition comprises two or more components which each
provides a source of protein, the total amount of protein will be
provided in the composition in a concentration of between 10-50% by
dry weight. In certain embodiments, the composition comprises two
or more components which each provide a source of protein, the
total amount of protein being in a ratio of between about 1:3
(protein:carbohydrate) and about 1:10 (protein:carbohydrate).
[0093] In certain embodiments, the total amount of protein may also
be in a ratio of between about 5:1 to about 1:5 (protein:fatty
acid). In some embodiments, the total amount of protein is in a
ratio of between about 5:1 and about 10:1.
[0094] Each component will provide a predetermined essential amino
acid profile. Aptly, the plurality 35 of components together
provide a predetermined essential amino acid profile in the final
product that approximates the amino acid profile seen in bee pupae
(Table 1).
[0095] Table 2 shows essential amino acid profiles of certain
components which may be comprised in the composition.
TABLE-US-00002 TABLE 2 Arq Hist lie Leu Lys Meth Phe Thr Val Bee
Bread 13.2 9.0 3.4 14.0 29.5 5.9 9.0 9.3 6.7 Bee Pupae 11.2 4.3 3.4
9.8 45.6 4.0 6.0 6.4 9.2 Royal Jelly 10.3 10.4 4.8 16.7 36.0 2.5
7.3 8.8 3.2 Blood meal 8.6 8.3 2.2 24.9 16.7 1.4 13.4 7.4 17.2 BSA
16.5 0.1 1.0 7.5 61.0 0.7 6.0 3.5 3.8 Canola protein 16.0 7.9 9.1
17.9 13.4 5.3 9.7 9.4 11.2 Casein 7.6 6.3 11.3 19.4 16.4 6.0 10.4
8.6 13.9 Egg white 11.3 4.7 9.8 16.2 12.7 10.6 11.4 9.4 13.9 Fish
meal 14.5 5.2 9.7 16.6 17.3 6.5 8.6 9.7 11.8 Hemp protein 29.3 7.4
6.5 14.9 9.0 5.3 10.3 8.6 8.7 Oat protein 10.1 3.2 5.7 10.9 6.1 3.7
7.8 4.8 47.6 Pea protein 18.6 5.5 9.6 17.8 15.8 2.5 11.5 8.0 10.6
Rice protein 18.6 5.1 9.8 18.7 6.8 6.6 12.8 8.5 13.0 Soy protein
17.4 6.0 11.1 17.7 13.9 3.0 12.0 8.2 10.7 Spirulina 13.8 3.5 13.0
20.2 9.3 5.0 10.7 10.1 14.3 Wheat gluten 10.2 5.4 13.0 22.9 4.4 4.1
14.0 8.9 17.1 Whey 3.8 2.8 12.0 21.9 20.8 3.6 5.5 17.0 12.6 Units
are mg/g
[0096] In certain embodiments of the present invention, the source
of protein also provides tryptophan.
[0097] In certain embodiments, the composition comprises a
combination of components which each provide a source of one or
more amino acids. Aptly, the combination of components together
provides a combination of amino acids which mimics the amino acids
found in bee pupae. Aptly, the composition comprises a combination
of components which together provide at least the following amino
acids (as a percentage of the total essential amino acids): [0098]
a) approximately 10 to 15% arginine; [0099] b) approximately 3 to
8% histadine; [0100] c) approximately 1 to 5% isoleucine; [0101] d)
approximately 8 to 13% leucine; [0102] e) approximately 40 to 50%
lysine; [0103] f) approximately 2 to 7% methionine; [0104] g)
approximately 3 to 8% phenylalanine; [0105] h) approximately 4 to
9% threonine; [0106] i) approximately 5 to 15% valine; and [0107]
j) approximately 5 to 10% tryptophan.
[0108] As noted above, the composition of certain embodiments of
the present invention comprises at least one component which is a
source of carbohydrate. Aptly, the composition comprises
carbohydrate in a concentration of between about 20 to about 90% of
the total dry weight of the composition. In certain embodiments,
the composition comprises a total amount of carbohydrate in a ratio
of between about 1:3 (protein:carbohydrate) to about 1:10
(protein:carbohydrate).
[0109] Aptly, the carbohydrate is provided in the composition at a
concentration of between about 20% to about 90%, e.g. between about
50% and about 90% by weight. Aptly, the composition comprises 20%,
25%, 30%, 35%, 40%, 45%, 50%, 55% 60%, 65%, 70%, 75% 80%, 85% or
90% by weight of carbohydrate.
[0110] The carbohydrate may be provided by one or more components.
Aptly, the carbohydrate is a mono-saccharide or a
di-saccharide.
[0111] In certain embodiments, the at least one component which is
a source of carbohydrate provides a carbohydrate selected from:
[0112] a) sucrose; [0113] b) glucose; [0114] c) fructose; [0115] d)
melezitose; [0116] e) sorbitol; [0117] f) mannitol; [0118] g)
trehalose; and [0119] h) maltose.
[0120] Aptly, the composition comprises one or more of a
carbohydrate selected from glucose, fructose and sucrose. In an
embodiment, the composition comprises fructose, sucrose and a
carbohydrate selected from glucose, melezitose and a sugar alcohol
e.g. sorbitol and mannitol. In an embodiment, the composition
comprises fructose, sucrose and glucose. In certain embodiments,
the composition comprises maltose e.g. in combination with one or
more of glucose, fructose or sucrose.
[0121] In certain embodiments, the at least one component which is
a source of carbohydrate provides sucrose, fructose and a
carbohydrate selected from glucose, melezitose, sorbitol and
mannitol, wherein the composition comprises fructose and the
carbohydrate selected from glucose, melezitose, sorbitol and
mannitol in equal proportions. In certain embodiments, the at least
one component which is a source of carbohydrate provides sucrose,
fructose and glucose, wherein the composition comprises fructose
and glucose in equal proportions.
[0122] In certain embodiments, the composition comprises sucrose,
glucose and fructose (e.g. a ratio of about 0:1:1 to about 1:0:0,
sucrose:glucose:fructose), wherein the composition comprises
fructose and glucose in equal proportions. In certain embodiments,
the composition comprises sucrose, glucose and fructose in a ratio
of about 0.5:1:1 to about 3:1:1 (sucrose:glucose:fructose) wherein
the composition comprises fructose and glucose in equal
proportions.
[0123] In certain embodiments, the composition comprises sucrose,
glucose and fructose between about 1:1:1 and about 3:1:1
(sucrose:glucose:fructose) wherein the composition comprises
fructose and glucose in equal proportions.
[0124] Aptly, the composition comprises fructose, sucrose and a
carbohydrate selected from glucose, melezitose and a sugar alcohol
e.g. sorbitol and mannitol in a predetermined ratio. In an
embodiment, the composition comprises fructose, sucrose and a
carbohydrate selected from glucose, melezitose and a sugar alcohol
e.g. sorbitol and mannitol in a ratio of between about O
(sucrose):1 (glucose):1 (fructose) to about 1 (sucrose):O
(glucose):O (fructose). Aptly, the composition comprises a ratio of
sucrose:glucose:fructose of between about 0.5:1:1 to about
3:1:1.
[0125] Aptly, the composition comprises a ratio of
sucrose:glucose:fructose of between about 1:0:0 to about 3:1:1,
e.g. 1:1:1, 1.5:1:1, 2:1:1, 2.5:1:1 or 3:1:1. In certain
embodiments of the invention, the composition comprises melezitose
and/or a sugar alcohol e.g. sorbitol and/or mannitol in place of
glucose.
[0126] In certain embodiments, the composition does not comprise a
complex carbohydrate e.g. starch.
[0127] Aptly, the carbohydrate is provided by dried sucrose,
glucose and/or fructose as a powder.
[0128] In an embodiment, the carbohydrate is provided by liquid
sucrose, glucose and/or fructose.
[0129] In certain embodiments, the composition comprises at least
one component which is a source of a fatty acid. Aptly, the
composition comprises at least one component which is a source of
an essential fatty acid. Aptly, the composition comprises one or
more components which provide a source of two essential fatty
acids.
[0130] It is considered that there are two essential fatty acids,
linoleic acid (an omega-6 fatty acid) and .alpha.-linolenic acid
(an omega-3 fatty acid). In certain embodiments, the composition
comprises a component or plurality of components which provide both
essential fatty acids. Thus, in certain embodiments, the
composition comprises linoleic acid (LA) and .alpha.-linolenic acid
(ALA).
[0131] Aptly, the composition comprises a plurality of components,
each of which provide at least one fatty acid. In certain
embodiments, the composition comprises a component which provides a
plurality of fatty acids. In an embodiment, the composition
comprises between about 1% and about 10% by weight of a fatty acid
or mixture of fatty acids. The one or more fatty acids may be
present in the composition in an amount of 1%, 2%, 3%, 4%, 5%, 6%,
7%, 8%, 9% or 10% by weight. In some embodiments, the composition
comprises greater than about 2% by weight of total fatty acid. In
some embodiments, the composition comprises greater than about 4%
by weight of total fatty acid.
[0132] It is considered that the ratio of omega-3 and omega-6 fatty
acids may be important in honey bee associative learning. Thus, in
certain embodiments, the composition comprises a high omega 3 fatty
acid:omega 6 fatty acid ratio and thus may improve honey bee
learning and cognitive performance. In certain embodiments, the
composition comprises components which provide a ratio of omega
3:omega 6 of less than 1:5, e.g. less than about 1:4, e.g. less
than 1:3, e.g. less than 1:2 for example 1:1.
[0133] In certain embodiments, the composition comprises one or
more components which provide a ratio of omega 3 fatty acid:omega 6
fatty acid of about 1:1 to about 3:1, for example 1:1 to 2:1.
[0134] It is considered that prior art compositions comprise a high
omega-6:omega 3 ratio, which may have detrimental effects on bee
learning i.e. greater than about 5:1 (omega 6:omega 3).
[0135] Aptly, the composition comprises linoleic acid and
.alpha.-linolenic acid in a ratio of between about 5:1 (linoleic
acid:.alpha.-linolenic acid) to 1:20 (linoleic
acid:.alpha.-linolenic acid). In one embodiment, the composition
comprises linoleic acid and .alpha.-linolenic acid in a ratio of
between about 5:1 (linoleic acid:.alpha.-linolenic acid) to 1:10
(linoleic acid:.alpha.-linolenic acid).
[0136] In certain embodiments, the composition comprises linoleic
acid and .alpha.-linolenic acid in a ratio of between about 2:1 to
about 1:5 (linoleic acid:.alpha.-linolenic acid). Aptly, the
composition comprises linoleic acid (LA) and .alpha.-linoleic acid
(ALA) in a ratio of (LA:ALA) in the range of 2:1 or less.
[0137] Aptly, the composition further comprises a fatty acid
selected from palmitic acid, myristic acid, lauric acid,
tricosanoic acid and arachidic acid. In certain embodiments, the at
least one component which is a source of one or more fatty acids
(FA) further provides palmitic acid, and oleic acid and
combinations thereof.
[0138] In certain embodiments, at least one component which is a
source of protein is not derived from egg yolk or corn gluten. In
certain embodiments, the composition is an aqueous composition.
[0139] The component which provides at least one fatty acid may be
an oil e.g. canola oil, Echium sp. oil, borage oil, flaxseed oil,
sage oil or hempseed oil or a mixture thereof. Dried lecithin from
soy or sunflower can also be used as a source of fatty acids.
[0140] Aptly, the composition comprises at least one sterol.
Insects require a dietary source of sterols; they cannot synthesize
sterols but need them for lipid membranes and hormone biosynthesis.
Bees acquire sterols from the pollen that they consume. Bees are
unique because unlike other insects, they have specific dietary
requirements for sterols. In particular, a dietary source of
24-methylene cholesterol (IUPAC name: ergosta-5,24(28)-dien-3 -ol)
may be important (Svoboda et al. 1986.). Other sterols derived from
pollen include campesterol, -sitosterol, and cholesterol.
[0141] Aptly, the at least one sterol is present in the composition
in an amount of about 0.01% to about 4%, e.g. 0.5%, 1%, 1.5%, 2%,
2.5%, 3%, 3.5% or 4%. In certain embodiments, the composition
comprises a plurality of sterols. If more than one sterol is
provided in the composition, the total combined amount of the
plurality of sterols is aptly between about 0.01% and about 4% by
weight of the composition. Aptly, the composition comprises one or
more sterols in a total concentration of between about 0.5% and
about 2% by dry weight of the composition.
[0142] At least one sterol may be selected from 24-methylene
cholesterol, campesterol, -sitosterol, and cholesterol or a
combination thereof. In certain embodiments, the composition
comprises 24-methylene cholesterol.
[0143] Aptly, the at least one sterol is a plurality of sterols,
wherein the plurality of sterols comprises at least two sterols
selected from 24-methylene cholesterol, campesterol, -sitosterol
and cholesterol. In certain embodiments, the composition comprises
24-methylene cholesterol, campesterol, -sitosterol, and
cholesterol. Aptly, the sterols are present in the composition in a
ratio of between about 1:1:1:1 to 10:1:1:1 (24-methylene
cholesterol:campesterol: -sitosterol:cholesterol).
[0144] Sources of sterols for use in the composition of certain
embodiments include for example, canola/oilseed rape oil, Echium
sp. oil, borage seed oil, marine algae, marine organisms and other
synthetic sources. Aptly, the composition comprises a component
selected from canola/oilseed rape oil, Echium sp. oil, borage seed
oil, and a component isolated from marine algae and a component
isolated form a marine organism.
[0145] In one embodiment, the at least one component which is a
source of a sterol is a sterol that has been extracted from one or
more of the above sources.
[0146] In certain embodiments, the sterol is derived from addition
of or extraction from Echium sp. Oil or Borago officianalis oil or
other oils or marine organisms.
[0147] In certain embodiments, the composition comprises one or
more vitamins and/or minerals. Bees have requirements for several
vitamins which they typically obtain from pollen. Diets deficient
in these vitamins can affect bee lifespan and performance.
Additionally, bees require dietary sources of salts and metals,
which they acquire from both nectar and pollen. However, too much
of these micronutrients can harm bees.
[0148] In certain embodiments, the composition further comprises at
least one vitamin. In certain embodiments, the at least one vitamin
is selected from one or more of Vitamin B1, Vitamin B2, Vitamin B3,
Vitamin B4, Vitamin B5, Vitamin B6, Vitamin B7, Vitamin B9,
inositol, Vitamin A, Vitamin C, Vitamin E and choline. Aptly, the
at least one vitamin is a plurality of vitamins, and wherein the
plurality of vitamins is selected from two or more of Vitamin B1,
Vitamin B2, Vitamin B3, Vitamin B4, Vitamin B5, Vitamin B6, Vitamin
B7, Vitamin B9, inositol, Vitamin A, Vitamin C, Vitamin E and
choline.
[0149] In certain embodiments, the at least one vitamin is provided
in the composition in a concentration of between about 0.0001% to
about 2% by dry weight of the composition, e.g. about 0.02% to
about 1% by dry weight of the composition.
[0150] In certain embodiments, the composition comprises at least
one mineral. Aptly, the at least one mineral is selected from zinc,
iron, manganese, magnesium, copper, potassium, phosphorous, calcium
and sodium. In certain embodiments, the at least one mineral is a
plurality of minerals and wherein the plurality of minerals is
selected from two or more of zinc, iron, manganese, magnesium,
copper, potassium, phosphorous, calcium and sodium.
[0151] In certain embodiments, the at least one mineral is provided
in the composition in a concentration of between about 0.05% and
about 2% by dry weight of the composition, e.g. between about 0.05%
and about 1% by dry weight of the composition.
[0152] Aptly, the composition comprises one or more vitamins and/or
minerals selected from the vitamins and mineral listed in Table 3.
Aptly, the vitamin and/or mineral is provided in the composition in
an amount indicated in Table 3. For example, in certain
embodiments, the composition comprises Vitamin B1 in an amount of
between about 1 to 25 .mu.g/g of the total dry weight of the
composition.
TABLE-US-00003 TABLE 3 Vitamins and minerals Component First range
Second Range Third Range Vitamins (.mu.g/g) B1 (thiamin) 1-25 6-20
6-20 B2 (riboflavin) 5-50 10-30 10-30 B3 (niacin) 50-350 100-300
100-300 B4 (nicotinic acid) 40-250 40-200 40-200
B5(pantothenicacid) 100-600 300-500 300-500 B6 (pyroxidine) 2-50
2-30 2-30 B9 (folic acid) 5-600 5-20 5-20 B7 (biotin) 0.5-2 0.5-2
0.5-2 B12 2-200 2-200 2-200 Inositol 2-2000 50-300 50-300 A
(retinal) 0.5-10 0.5-10 0.5-10 C (ascorbic acid) 100-2000 150-600
150-600 E (tocopherol) 0.3-50 10-40 10-40 Choline 50-5000 500-5000
500-5000 Minerals/Metals (mg/g) Zinc 0.030-0.250 0.030-0.250
0.030-0.250 Iron 0.011-0.170 0.011-0.170 0.011-0.170 Manganese
0.020-0.110 0.020-0.110 0.020-0.110 Magnesium 0.7-1.48 0.7-1.48
0.7-1.48 Copper 0.002-0.016 0.002-0.016 0.002-0.016 Potassium 4-20
4-20 4-20 Phosphorous 0.8-6 0.8-6 0.8-6 Calcium 0.2-3 0.2-3 0.2-3
Sodium 0.006-0.6 0.006-0.6 0.006-0.6
[0153] In certain embodiments, the composition comprises at least
one component which is a source of a phenolic compound. It is
believed that phenolic compounds may improve palatability of the
composition.
[0154] Aptly, the composition comprises at least one component
which is a source of a carotenoid compound. Bee collected pollen
contains a large variety of phenolic compounds (e.g.
hydroxycinnamic acids and flavonoids) and carotenoid compounds made
by plants. Some of these compounds are anti-oxidant and provide
protection against free radicals. Phenolic compounds have been
reported as a major component of the pollen of most species of
plants and range in concentration from 0.025 to 25 mg/g dry weight
and occasionally higher.
[0155] Aptly, the at least one phenolic compound is selected from a
hydroxycinnamic acid and a flavonoid. Aptly, the phenolic compound
is selected from rutin, quercetin, naringenin, ferulic acid,
p-coumaric acid, caffeic acid and kaempferol.
[0156] In certain embodiments, the composition further comprises
ferulic acid, p-coumaric acid, caffeic acid and kaempferol, wherein
each of ferulic acid, p-coumaric acid, caffeic acid and kaempferol
is provided in the composition at a ratio to rutin of 1:10.
[0157] Aptly, the composition comprises at least one phenolic
compound selected from ferulic acid, p-coumaric acid, caffeic acid
or their derivatives, or a flavonoid from quercetin, naringenin,
kaempferol or their glycosides and combinations thereof.
[0158] In certain embodiments, the at least one phenolic compound
is a plurality of phenolic compounds selected from two or more of
rutin, quercetin, naringenin, ferrulic acid, p-coumaric acid,
caffeic acid and kaempferol. Aptly, the composition comprises
rutin, quercetin and naringenin in equal concentration.
[0159] In certain embodiments, the composition comprises
kaempferol, quercetin and naringenin or their glycosides in equal
ratios. Aptly, kaempferol, quercetin and naringenin or their
glycosides are present in the composition in an amount of
approximately 0.2% by weight.
[0160] In some embodiments, the composition further comprises
p-coumaric acid, caffeic acid and a flavonoid glycoside. Aptly,
each of the aforementioned phenolic compounds are provided in an
amount which is approximately a tenth of the amount of rutin
provided in the composition. Aptly, p-coumaric acid, caffeic acid
or their derivatives and the flavonoid glycoside are each provided
in an amount of approximately 0.02% by weight.
[0161] In certain embodiments, the composition further comprises a
component which is a source of a carotenoid compound. Exemplary
carotenoid compounds include for example lutein, -cryptoxanthin and
-carotene. Aptly, the at least one carotenoid compound is selected
from lutein, -cryptoxanthin, and -carotene. Aptly, the composition
comprises lutein, -cryptoxanthin, and -carotene. Aptly, the lutein,
-cryptoxanthin, and -carotene are provided in the composition in
equal respective amounts to a total amount of about 0.2 mg/g
(0.002% by weight).
[0162] Aptly, the composition comprises lutein, -cryptoxanthin, and
-carotene. In certain embodiments, each carotenoid compound is
provided in the composition in a concentration which is equal to a
concentration of one or more respective other carotenoid
compounds.
[0163] In certain embodiments, the composition comprises at least
one scent compound.
[0164] Most pollens emit a scent in the form of aldehyde and ketone
fatty acid derivatives, mono and sesquiterpenes, or phenolics such
as benzaldehyde and eugenol (Dobson and Bergstrom, 2000). Some of
these compounds are attractive to foraging bees and indicate the
presence of pollen. These compounds are also found in honey
produced by bees.
[0165] Aptly, the at least one scent compound is selected from
eugenol, phenylacetaldehyde, linalool, furfural, -carophyllene,
-damascenone and 1-hexanol. Aptly, the at least one scent compound
is a plurality of scent compounds selected from two or more of
eugenol, phenylacetaldehyde, linalool, furfural, -carophyllene,
-damascenone and 1-hexanol. In certain embodiments, each of the one
or more scent compounds is provided in a concentration which is
equal to a concentration of one or more respective other scent
compounds.
[0166] In certain embodiments, the one or more scent compounds are
provided in the composition in a total amount of about 0.001% of
the final dry weight of the composition.
[0167] In other embodiments, the composition may further comprise
one or more additional essential oils including for example neroli
oil and/or orange or rose flower water extractions. Such
extractions are commercially available.
[0168] In one embodiment, the composition further comprises water.
The amount of water added to the composition will depend on the
specific embodiment of the composition and the components provided
in the composition.
[0169] In certain embodiments of the present invention, the
composition further comprises glycerol. The composition of certain
embodiments of the present invention may therefore comprise a
plurality of components in predetermined ratios and amounts. Aptly,
the composition comprises a plurality of components selected from:
[0170] a) a source of protein; [0171] b) a source of carbohydrate;
[0172] c) a source of fatty acids, wherein aptly the fatty acids
are provided in a ratio of 5:1 (omega 6 fatty acid:omega 3 fatty
acid) to 1:20 (omega 6 fatty acid:omega 3 fatty acid); [0173] d) a
source of a sterol; [0174] e) a source of one or more vitamins
and/or minerals; [0175] f) a source of one or more phenolic
compounds; [0176] g) a source of one or more carotenoids; and
[0177] h) a source of one or more scent compounds.
[0178] In one embodiment, the composition comprises the components
listed in Table 4 below:
TABLE-US-00004 TABLE 4 Recipe for dry ingredients Component First
range SecondRange Third Range Carbohydrate 20-90% 50-90% 50-90%
Suc:gluc:fruct 0:1:1 to 1:0:0 1:0.5:0.5 to 1:0:0 to 3:1:1 3:1:1
Protein 10-50% 10-30% 10-30% Fatty acids 1-30% 1-10% 1-8% LA:ALA
5:1 to 1:20 2:1 to 1:10 1:1 to 1:10 Sterols 0%-4% 0.5-2% 0.5-2%
24M:CMP: 1:1:1:1 to 1:1:1:1 to 1:1:1:1 to CHO:BST 10:1:1:1 10:1:1:1
10:1:1:1 Vitamins 0.001-2% 0.02-2% 0.02-1% Minerals 0.05-6% 0.05-1%
0.05-1% Phenolics 0-12% 1-8% 2-5% Carotenoids 0-0.05% 0.007-0.05%
0.007-0.05% Scent 0-0.05% 0.001-0.05% 0.001-0.05% All are % dry
weight of mixture. Note: this table does not include water. Amount
of water added to final formulation is determined by specific
embodiments of the invention. LA = linoleic acid, ALA =
.alpha.-linolenic acid 24M = 24-methylene cholesterol, CMP =
campesterol, CHO = cholesterol, BST = .beta.-sitosterol
[0179] In certain embodiments, a product comprising the composition
may be prepared as follows:
[0180] Method 1
[0181] As a first step, all dry ingredients are combined.
Subsequently, oils e.g. oils which are components which provide one
or more fatty acids and/or one or more sterols are mixed together.
These are then cut into the dry ingredients using a blending method
to render a fine crumb. Finally, water should be added to yield a
dry, slightly sticky dough. The dough can then be shaped into
patties. In certain embodiments, glycerol may be added to keep the
patties moist and easy for bees to consume.
[0182] Aptly, the composition is formulated into a "raw" i.e.
non-baked product.
[0183] Method 2
[0184] As a first step, all dry ingredients are combined together
with a portion of the carbohydrates. Aptly, a third of the
carbohydrate is added to the dry components.
[0185] Baking powder and baking soda (sodium bicarbonate) is then
added to the dry component/carbohydrate material. Approximately,
fifteen grams of baking powder and fifteen grams of baking soda
(sodium bicarbonate) may be added to every 400 grams of dry
component material.
[0186] In a subsequent step, components which are oils e.g. oils
which provide fatty acids and sterols should be mixed together,
then cut into the dry ingredients using a blending method to render
a fine crumb. 100 ml of water is added to every 400 g of dry
material and mixed thoroughly. The resulting batter is placed in a
pan to approximately 1-2.5 cm deep and baked for 40-60 min at
120.degree. C.
[0187] After baking, the resulting biscuit is removed from the oven
and holes should be pricked in the surface. While the biscuit is
still warm, 60% sugar syrup is poured over it, covering the entire
surface and saturating the biscuit.
[0188] Aptly, the composition is provided as a baked product e.g. a
biscuit. In certain embodiments, the patty or biscuit has a large
surface to volume ratio. For example, the patty or biscuit may have
dimensions of 200 mm.times.200 mm.times.6 mm.
[0189] The composition according to certain embodiments of the
present invention may be used to supply nutritional support to a
bee. Aptly, the bee may be in a colony of bees e.g. in a hive.
[0190] Aptly, the composition increases honey production by a
colony of bees fed on the composition. Aptly the composition is fed
to a colony of bees in the absence of pollen.
[0191] Aptly, the composition is fed to a colony of bees as a
supplement to naturally available pollen. In certain embodiments,
the composition is a liquid.
[0192] The composition may be fed to a colony of bees in a variety
of ways. For example, the composition may be a liquid and fed
within a hive in a horizontal feeder in place of a comb.
Alternatively, or in addition, the composition may be placed in a
vertical feeder which is in turn placed on top of a comb within the
hive. The composition may be a liquid, a patty or a biscuit.
[0193] In certain embodiments, the composition is provided adjacent
to a comb in the hive e.g. on top of the comb. Aptly, the
composition is provided on a mesh through which the bees can pass.
The composition may be a liquid, a patty or a biscuit.
[0194] In certain embodiments, the composition is provided in an
inverted jar inserted into a hole in the roof of a hive. Aptly, the
composition is liquid.
[0195] In certain embodiments, the composition may be provided in
an area surrounding a hive (within an apiary). Thus, the bees may
eat the composition as part of their foraging. Aptly, the
composition may be in the form of a liquid or a powder.
EXAMPLES
Example 1
[0196] The composition is tested on nucleus honey bee colonies.
[0197] Methods. Feeding Assays with Whole Colonies of Bees.
[0198] Nucleus honey bee colonies containing 4-5 frames of bees,
including two frames with brood, can be placed separately in netted
enclosures (2.5.times.4.times.2 m) according to a randomized block
design. The hives may be placed inside the enclosures for a week
without additional food in order to finish their pollen reserves.
Then each hive can forage from a dish in the enclosure that
contained 30 g of a composition according to certain embodiments of
the present invention every day, and leftover food from the day
before can be removed and weighed. Each hive may have inside the
enclosure ad lib access to 30-50% sucrose solution and fresh water.
All hives are inside the enclosures for eight weeks.
[0199] In an alternative embodiment, the composition may be
supplied to individual bees as described below:
[0200] Method 1. Individual Bee Tests.
[0201] To measure the food consumption and behaviour of individual
bees the methods previously published in Stabler et al. (2015) may
be used. In summary, bees can be housed individually in a plastic
box (16.5.times.11.times.6.5 cm) with 20 holes (2 mm) drilled at
each end of the lid for ventilation. In three sides of the box, a
hole can be cut to insert a 2 ml microcentrifuge tube; each tube
may have four holes (2 mm) drilled in a line in one side of the
tube to facilitate feeding by the bees.
[0202] Two of the tubes may be filled with a liquid composition
according to certain embodiments of the present invention; the
remaining tube can then be filled with deionised water. A piece of
absorbent laboratory paper may be added to the housing box,
covering the base. After being placed in the box, bees are left to
acclimatise at room temperature before the feeding solutions are
added. Bees may then be moved into the 28.degree. C. controlled
temperature room or incubator and kept in darkness for 7 days,
through the course of the experiment. After use in treatments, bees
are frozen at -20.degree. C. to euthanize. Each tube containing the
solution is weighed before being placed in the arena and 24 h
afterwards.
[0203] A further alternative method to determine food consumption
and behaviour of bees is as follows:
[0204] Method 2. Cohorts of Bees.
[0205] To measure the feeding and survival of groups of bees,
methods previously published in Paoli et al. (2014) can be used. In
short, newly-emerged honeybees are collected from frames and 20-30
bees placed in a Perspex box (11.times.6.times.20 cm). The boxes
are placed in a constant temperature room at 34.degree. C. and 60%
relative humidity.
[0206] Experiments continue for 7-14 days. The number of dead bees
in each box is counted daily to measure survival. Bees may be fed
using feeding tubes consisting of 2 ml microcentrifuge tubes (four
3 mm holes were drilled along the top of each tube). Four feeding
tubes are filled with a solution comprising a composition according
to certain embodiments of the present invention; each box also has
a water tube. In the case of solid compositions according to
certain embodiments of the present invention, bees can be fed with
modified microcentrifuge tubes. Each tube may contain a 1
cm.times.2 cm hole cut on the top surface. Food can be dried in an
oven at 40 C, and then packed into the tube, the tube weighed, and
then placed in the rearing box for 24 h. After 24 h, each tube may
be placed in the 40.degree. C. oven for 24 h, and the tube was
reweighed.
[0207] Throughout the description and claims of this specification,
the words "comprise" and "contain" and variations of them mean
"including but not limited to" and they are not intended to (and do
not) exclude other moieties, additives, components, integers or
steps. Throughout the description and claims of this specification,
the singular encompasses the plural unless context otherwise
requires. In particular, where the indefinite article is used, the
specification is to be understood as contemplating plurality as
well as singularity, unless the context requires otherwise.
[0208] Features, integers, characteristics or groups described in
conjunction with a particular aspect, embodiment or example of the
invention are to be understood to be applicable to any other
aspect, embodiment or example described herein unless incompatible
therewith. All of the features disclosed in this specification
(including any accompanying claims, abstract and drawings), and/or
all of the steps of any method or process so disclosed, may be
combined in any combination, except combinations where at least
some of the features and/or steps are mutually exclusive. The
invention is not restricted to any details of any foregoing
embodiments. The invention extends to any novel one, or novel
combination, of the features disclosed in this specification
(including any accompanying claims, abstract and drawings), or to
any novel one, or any novel combination, of the steps of any method
or process so disclosed.
[0209] The reader's attention is directed to all papers and
documents which are filed concurrently with or previous to this
specification in connection with this application and which are
open to public inspection with this specification, and the contents
of all such papers and documents are incorporated herein by
reference.
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