Methods for Producing Corn Plants with Northern Leaf Blight Resistance and Compositions Thereof
BOURDONCLE; William J. ; et al.
U.S. patent application number 17/537900 was filed with the patent office on 2022-03-24 for methods for producing corn plants with northern leaf blight resistance and compositions thereof. This patent application is currently assigned to Monsanto Technology LLC. The applicant listed for this patent is Monsanto Technology LLC. Invention is credited to William J. BOURDONCLE, Franck J. CHOPIN, Romain FOUQUET, Marcelo P. GIOVANINI, Peter V. MALONEY, Yule PAN.
| Application Number | 20220087187 17/537900 |
| Document ID | / |
| Family ID | 1000005996714 |
| Filed Date | 2022-03-24 |
| United States Patent Application | 20220087187 |
| Kind Code | A1 |
| BOURDONCLE; William J. ; et al. | March 24, 2022 |
Methods for Producing Corn Plants with Northern Leaf Blight Resistance and Compositions Thereof
Abstract
The present disclosure is in the field of plant breeding and disease resistance. The disclosure provides methods for breeding corn plants having northern leaf blight (NLB) resistance using marker-assisted selection. The disclosure further provides corn germplasm resistant to NLB. The disclosure also provides markers associated with NLB resistance loci for introgressing these loci into elite germplasm in a breeding program, thus producing novel NLB resistant germplasm.
| Inventors: | BOURDONCLE; William J.; (Borken, DE) ; CHOPIN; Franck J.; (Boucau, FR) ; FOUQUET; Romain; (Saint-Palais, FR) ; GIOVANINI; Marcelo P.; (Londrina, BR) ; MALONEY; Peter V.; (Galena, MD) ; PAN; Yule; (Chesterfield, MO) | ||||||||||
| Applicant: |
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| Assignee: | Monsanto Technology LLC St. Louis MO |
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| Family ID: | 1000005996714 | ||||||||||
| Appl. No.: | 17/537900 | ||||||||||
| Filed: | November 30, 2021 |
Related U.S. Patent Documents
| Application Number | Filing Date | Patent Number | ||
|---|---|---|---|---|
| 16877649 | May 19, 2020 | 11219174 | ||
| 17537900 | ||||
| 15382229 | Dec 16, 2016 | 10694693 | ||
| 16877649 | ||||
| 62269635 | Dec 18, 2015 | |||
| Current U.S. Class: | 1/1 |
| Current CPC Class: | A01H 6/4684 20180501; A01H 1/04 20130101; C12Q 2600/13 20130101; A01H 1/02 20130101; C12Q 1/6895 20130101; C12Q 2600/156 20130101; A01H 5/10 20130101 |
| International Class: | A01H 6/46 20060101 A01H006/46; A01H 1/02 20060101 A01H001/02; A01H 1/04 20060101 A01H001/04; A01H 5/10 20060101 A01H005/10; C12Q 1/6895 20060101 C12Q001/6895 |
Claims
1-26. (canceled)
27. A method of creating a population of Northern Leaf Blight (NLB) resistant corn plants, corn seeds, or corn cells, said method comprising: a) crossing a first NLB resistant corn plant comprising NLB resistance quantitative trait locus (QTL) NLB_4.02 with a second corn plant to generate a first population of corn plants, corn seeds, or corn cells; b) genotyping said first population of corn plants, corn seeds, or corn cells at one or more marker loci linked to, and within 10 centimorgans (cM) of, said NLB resistance QTL NLB_4.02; and c) selecting from said first population one or more corn plants, corn seeds, or corn cells having resistance to NLB comprising said one or more of said marker loci linked to said NLB resistance QTL NLB_4.02 linked to an NLB resistance allele selected from the group consisting of: SEQ ID NO: 33 comprising a G, at position number 319; SEQ ID NO: 35 comprising a G, at position number 101; SEQ ID NO: 36 comprising an A, at position number 373; SEQ ID NO: 37 comprising an A, at position number 115; SEQ ID NO: 38 comprising a G, at position number 171; SEQ ID NO: 39 comprising a T, at position number 37; SEQ ID NO: 40 comprising a C, at position number 101; SEQ ID NO: 41 comprising a G, at position number 101; SEQ ID NO: 42 comprising a T, at position number 101; SEQ ID NO: 43 comprising a T, at position number 101; SEQ ID NO: 44 comprising a G or a T, at position number 2239; SEQ ID NO: 45 comprising an A, at position number 569; SEQ ID NO: 46 comprising a T, at position number 101; SEQ ID NO: 47 comprising a C, at position number 240; SEQ ID NO: 48 comprising a C, at position number 247; SEQ ID NO: 49 comprising an A, at position number 719; SEQ ID NO: 50 comprising an A, at position number 429; SEQ ID NO: 51 comprising an A, at position number 101; SEQ ID NO: 52 comprising a C, at position number 81; SEQ ID NO: 471 comprising a G, at position number 173; SEQ ID NO: 472 comprising a T, at position number 101; SEQ ID NO: 473 comprising a G, at position number 101; SEQ ID NO: 474 comprising a G, at position number 101; and SEQ ID NO: 475 comprising an A, at position number 101.
28. The method of claim 27, wherein said selecting comprises selecting one or more marker loci located in a chromosomal interval flanked by any two of SEQ ID NOs: 32 to 52 and 471 to 475, and said NLB resistance allele is at said QTL NLB_4.02.
29. The method of claim 27, wherein said selecting comprises selecting one or more marker loci are located in a chromosomal interval flanked by any two of SEQ ID NOs: 33 to 42, 473 and 474, and said NLB resistance allele is at said QTL NLB_4.02.
30. The method of claim 27, wherein said selecting comprises selecting one or more marker loci are located in a chromosomal interval flanked by any two of SEQ ID NOs: 43 to 49, and said NLB resistance allele is at said QTL NLB_4.02.
31. The method of claim 27, wherein said selecting comprises selecting one or more marker loci are linked to, and within 5 cM of, any one of the marker loci selected from the group consisting of SEQ ID NOs: SEQ ID NOs: 32 to 52 and 471 to 475, and said NLB resistance allele is at said QTL NLB_4.02.
32. The method of claim 27, wherein said selected plant comprises NLB resistance QTL NLB_4.02 and mild resistance or resistance to infection by Exserohilum turcicum.
33. The method of claim 27, wherein said selected one or more corn plants exhibits reduced root rot, reduced stalk rot, fewer leaf lesions, less foliage destruction, or any combination thereof, compared to corn plants lacking said NLB resistance QTL NLB_4.02 when grown under a high NLB stress condition.
34. The method of claim 27, wherein said NLB resistance QTL NLB_4.02 does not confer a yield penalty when grown under a low NLB stress condition.]
35. The method of claim 27, wherein said method further comprises: producing from said one or more corn plants, corn seeds, or corn cells selected in step c) a second population of corn plants, corn seeds, or corn cells comprising said NLB resistance QTL NLB_4.02.
36. The method of claim 35, wherein said second population of corn plants or seeds exhibits a reduction of NLB rating score of 1 or more as compared to corn plants or seeds lacking said NLB resistance QTL NLB_4.02 when grown under a high NLB stress condition.
37. The method of claim 35, wherein said second population of corn plants or seeds exhibits a reduction of NLB rating score of 2 or more as compared to corn plants or seeds lacking said NLB resistance QTL NLB_4.02 when grown under a high NLB stress condition.
38. The method of claim 27, wherein said one or more marker loci are selected from the group consisting of: SEQ ID NO: 33 comprising a G, at position number 319; SEQ ID NO: 35 comprising a G, at position number 101; SEQ ID NO: 36 comprising an A, at position number 373; SEQ ID NO: 37 comprising an A, at position number 115; SEQ ID NO: 38 comprising a G, at position number 171; SEQ ID NO: 39 comprising a T, at position number 37; SEQ ID NO: 40 comprising a C, at position number 101; SEQ ID NO: 41 comprising a G, at position number 101; SEQ ID NO: 42 comprising a T, at position number 101; SEQ ID NO: 43 comprising a T, at position number 101; SEQ ID NO: 44 comprising a G or a T, at position number 2239; SEQ ID NO: 45 comprising an A, at position number 569; SEQ ID NO: 46 comprising a T, at position number 101; SEQ ID NO: 47 comprising a C, at position number 240; SEQ ID NO: 48 comprising a C, at position number 247; SEQ ID NO: 49 comprising an A, at position number 719; SEQ ID NO: 50 comprising an A, at position number 429; SEQ ID NO: 51 comprising an A, at position number 101; SEQ ID NO: 52 comprising a C, at position number 81; SEQ ID NO: 471 comprising a G, at position number 173; SEQ ID NO: 472 comprising a T, at position number 101; SEQ ID NO: 473 comprising a G, at position number 101; SEQ ID NO: 474 comprising a G, at position number 101; and SEQ ID NO: 475 comprising an A, at position number 101.
39. The method of claim 27, wherein said selecting comprises selecting one or more corn plants, corn seeds, or corn cells that do not comprise NLB resistance quantitative trait loci (QTL) NLB_4.01.
40. The method of claim 27, further comprising selecting one or more corn plants, corn seeds, or corn cells comprising NLB resistance quantitative trait loci (QTL) NLB_4.01.
41. A method of creating a population of Northern Leaf Blight (NLB) resistant corn plants, corn seeds, or corn cells, said method comprising: a) crossing a first NLB resistant corn plant comprising an NLB resistance quantitative trait loci (QTL) NLB_4.02 with a second corn plant to generate a first population of corn plants, corn seeds, or corn cells; b) genotyping said first population of corn plants, corn seeds, or corn cells at one or more marker loci linked to, and within 10 centimorgans (cM) of, any one of the marker loci selected from the group consisting of SEQ ID NOs: 32 to 52 and 471 to 475, wherein said marker is associated with said NLB resistance QTL NLB_4.02; and c) selecting from said first population one or more corn plants, corn seeds, or corn cells having resistance to NLB comprising said one or more of said marker loci linked to said NLB resistance QTL NLB_4.02 linked to an NLB resistance allele selected from the group consisting of: SEQ ID NO: 33 comprising a G, at position number 319; SEQ ID NO: 35 comprising a G, at position number 101; SEQ ID NO: 36 comprising an A, at position number 373; SEQ ID NO: 37 comprising an A, at position number 115; SEQ ID NO: 38 comprising a G, at position number 171; SEQ ID NO: 39 comprising a T, at position number 37; SEQ ID NO: 40 comprising a C, at position number 101; SEQ ID NO: 41 comprising a G, at position number 101; SEQ ID NO: 42 comprising a T, at position number 101; SEQ ID NO: 43 comprising a T, at position number 101; SEQ ID NO: 44 comprising a G or a T, at position number 2239; SEQ ID NO: 45 comprising an A, at position number 569; SEQ ID NO: 46 comprising a T, at position number 101; SEQ ID NO: 47 comprising a C, at position number 240; SEQ ID NO: 48 comprising a C, at position number 247; SEQ ID NO: 49 comprising an A, at position number 719; SEQ ID NO: 50 comprising an A, at position number 429; SEQ ID NO: 51 comprising an A, at position number 101; SEQ ID NO: 52 comprising a C, at position number 81; SEQ ID NO: 471 comprising a G, at position number 173; SEQ ID NO: 472 comprising a T, at position number 101; SEQ ID NO: 473 comprising a G, at position number 101; SEQ ID NO: 474 comprising a G, at position number 101; and SEQ ID NO: 475 comprising an A, at position number 101.
42. The method of claim 27, wherein said selecting comprises selecting a first or second corn plant comprises an NLB resistance QTL selected from NLB_2.01, NLB_3.01, NLB_6.01, NLB_7.01, or NLB_9.01, wherein said NLB resistance QTL is linked to a second NLB resistance allele from the group consisting of: SEQ ID NO: 1 comprising an A, at position number 101; SEQ ID NO: 2 comprising a G, at position number 101; SEQ ID NO: 3 comprising an A, at position number 101; SEQ ID NO: 4 comprising an A, at position number 77; SEQ ID NO: 5 comprising a T, at position number 101; SEQ ID NO: 6 comprising a G, at position number 136; SEQ ID NO: 7 comprising a T, at position number 104; SEQ ID NO: 8 comprising an A, at position number 112; SEQ ID NO: 9 comprising a T, at position number 902; SEQ ID NO: 10 comprising a G, at position number 101; SEQ ID NO: 11 comprising an A, at position number 205; SEQ ID NO: 12 comprising an A, at position number 245; SEQ ID NO: 13 comprising a G, at position number 43; SEQ ID NO: 14 comprising a G, at position number 144; SEQ ID NO: 15 comprising a C, at position number 101; SEQ ID NO: 16 comprising a T, at position number 247; SEQ ID NO: 17 comprising an A, at position number 341; SEQ ID NO: 18 comprising a C, at position number 91; SEQ ID NO: 19 comprising a T, at position number 216; SEQ ID NO: 20 comprising a G, at position number 81; SEQ ID NO: 21 comprising a G, at position number 194; SEQ ID NO: 22 comprising an A, at position number 46; SEQ ID NO: 23 comprising a G, at position number 859; SEQ ID NO: 24 comprising a T, at position number 200; SEQ ID NO: 25 comprising a C, at position number 73; SEQ ID NO: 26 comprising a T, at position number 352; SEQ ID NO: 27 comprising a T, at position number 162; SEQ ID NO: 28 comprising a T, at position number 106; SEQ ID NO: 29 comprising a C, at position number 319; SEQ ID NO: 30 comprising a G, at position number 127; SEQ ID NO: 31 comprising a T, at position number 101; SEQ ID NO: 65 comprising a G, at position number 101; SEQ ID NO: 67 comprising a C, at position number 101; SEQ ID NO: 68 comprising a T, at position number 101; SEQ ID NO: 69 comprising a G, at position number 101; SEQ ID NO: 70 comprising a T, at position number 101; SEQ ID NO: 71 comprising a T, at position number 101; SEQ ID NO: 72 comprising a G, at position number 279; SEQ ID NO: 73 comprising a G, at position number 265; SEQ ID NO: 74 comprising a T, at position number 101; SEQ ID NO: 75 comprising a C, at position number 101; SEQ ID NO: 76 comprising a C, at position number 209; SEQ ID NO: 77 comprising a G, at position number 256; SEQ ID NO: 78 comprising a T, at position number 101; SEQ ID NO: 79 comprising an A, at position number 101; SEQ ID NO: 80 comprising an A, at position number 91; SEQ ID NO: 81 comprising an A, at position number 47; SEQ ID NO: 82 comprising a T, at position number 321; SEQ ID NO: 83 comprising a C, at position number 101; SEQ ID NO: 84 comprising a G, at position number 474; SEQ ID NO: 85 comprising a T, at position number 101; SEQ ID NO: 86 comprising a C, at position number 101; SEQ ID NO: 87 comprising a C, at position number 101; SEQ ID NO: 88 comprising a G, at position number 49; SEQ ID NO: 89 comprising an A, at position number 223; SEQ ID NO: 469 comprising a T, at position number 191; SEQ ID NO: 470 comprising a G, at position number 426; SEQ ID NO: 476 comprising a G, at position number 101; SEQ ID NO: 477 comprising a C, at position number 101; SEQ ID NO: 478 comprising a G, at position number 412; SEQ ID NO: 479 comprising a C, at position number 444; SEQ ID NO: 480 comprising a C, at position number 101; SEQ ID NO: 481 comprising an A, at position number 101; and SEQ ID NO: 482 comprising a C, at position number 101.
43. The method of claim 27, wherein said selecting comprises selecting a first or second corn plant comprises an NLB resistance QTL selected from NLB_2.01, NLB_3.01, NLB_6.01, NLB_7.01, or NLB_9.01, wherein said NLB resistance QTL is linked to a second NLB resistance allele from the group consisting of: SEQ ID NO: 1 comprising an A, at position number 101; SEQ ID NO: 2 comprising a G, at position number 101; SEQ ID NO: 3 comprising an A, at position number 101; SEQ ID NO: 4 comprising an A, at position number 77; SEQ ID NO: 5 comprising a T, at position number 101; SEQ ID NO: 6 comprising a G, at position number 136; SEQ ID NO: 7 comprising a T, at position number 104; SEQ ID NO: 8 comprising an A, at position number 112; SEQ ID NO: 9 comprising a T, at position number 902; SEQ ID NO: 10 comprising a G, at position number 101; SEQ ID NO: 11 comprising an A, at position number 205; SEQ ID NO: 12 comprising an A, at position number 245; SEQ ID NO: 13 comprising a G, at position number 43; SEQ ID NO: 14 comprising a G, at position number 144; SEQ ID NO: 15 comprising a C, at position number 101; SEQ ID NO: 16 comprising a T, at position number 247; SEQ ID NO: 17 comprising an A, at position number 341; SEQ ID NO: 18 comprising a C, at position number 91; SEQ ID NO: 19 comprising a T, at position number 216; SEQ ID NO: 20 comprising a G, at position number 81; SEQ ID NO: 21 comprising a G, at position number 194; SEQ ID NO: 22 comprising an A, at position number 46; SEQ ID NO: 23 comprising a G, at position number 859; SEQ ID NO: 24 comprising a T, at position number 200; SEQ ID NO: 25 comprising a C, at position number 73; SEQ ID NO: 26 comprising a T, at position number 352; SEQ ID NO: 27 comprising a T, at position number 162; SEQ ID NO: 28 comprising a T, at position number 106; SEQ ID NO: 29 comprising a C, at position number 319; SEQ ID NO: 30 comprising a G, at position number 127; SEQ ID NO: 31 comprising a T, at position number 101; SEQ ID NO: 65 comprising a G, at position number 101; SEQ ID NO: 67 comprising a C, at position number 101; SEQ ID NO: 68 comprising a T, at position number 101; SEQ ID NO: 69 comprising a G, at position number 101; SEQ ID NO: 70 comprising a T, at position number 101; SEQ ID NO: 71 comprising a T, at position number 101; SEQ ID NO: 72 comprising a G, at position number 279; SEQ ID NO: 73 comprising a G, at position number 265; SEQ ID NO: 74 comprising a T, at position number 101; SEQ ID NO: 75 comprising a C, at position number 101; SEQ ID NO: 76 comprising a C, at position number 209; SEQ ID NO: 77 comprising a G, at position number 256; SEQ ID NO: 78 comprising a T, at position number 101; SEQ ID NO: 79 comprising an A, at position number 101; SEQ ID NO: 80 comprising an A, at position number 91; SEQ ID NO: 81 comprising an A, at position number 47; SEQ ID NO: 82 comprising a T, at position number 321; SEQ ID NO: 83 comprising a C, at position number 101; SEQ ID NO: 84 comprising a G, at position number 474; SEQ ID NO: 85 comprising a T, at position number 101; SEQ ID NO: 86 comprising a C, at position number 101; SEQ ID NO: 87 comprising a C, at position number 101; SEQ ID NO: 88 comprising a G, at position number 49; SEQ ID NO: 89 comprising an A, at position number 223; SEQ ID NO: 469 comprising a T, at position number 191; SEQ ID NO: 470 comprising a G, at position number 426; SEQ ID NO: 476 comprising a G, at position number 101; SEQ ID NO: 477 comprising a C, at position number 101; SEQ ID NO: 478 comprising a G, at position number 412; SEQ ID NO: 479 comprising a C, at position number 444; SEQ ID NO: 480 comprising a C, at position number 101; SEQ ID NO: 481 comprising an A, at position number 101; and SEQ ID NO: 482 comprising a C, at position number 101.
44. The method of claim 42, wherein said NLB resistance QTL is NLB_2.01.
45. The method of claim 42, wherein said NLB resistance QTL is NLB_3.01.
46. The method of claim 42, wherein said NLB resistance QTL is NLB_6.01.
47. The method of claim 42, wherein said NLB resistance QTL is NLB_7.01.
48. The method of claim 42, wherein said NLB resistance QTL is NLB_9.01.
49. The method of claim 43, wherein said NLB resistance QTL is NLB_2.01.
50. The method of claim 43, wherein said NLB resistance QTL is NLB_3.01.
51. The method of claim 43, wherein said NLB resistance QTL is NLB_6.01.
52. The method of claim 43, wherein said NLB resistance QTL is NLB_7.01.
53. The method of claim 43, wherein said NLB resistance QTL is NLB_9.01.
Description
CROSS REFERENCE TO RELATED APPLICATIONS
[0001] This application is a divisional of U.S. application Ser. No. 16/877,649, filed May 19, 2020, which is a divisional of U.S. application Ser. No. 15/382,229, filed Dec. 16, 2016 (now U.S. Pat. No. 10,694,693, issued Jun. 30, 2020), which claims the benefit and priority of U.S. Provisional Application No. 62/269,635 filed Dec. 18, 2015, which are incorporated by reference in their entireties.
FIELD
[0002] The present disclosure relates to the field of agricultural biotechnology. More specifically, this disclosure relates to methods for producing corn plants, seeds, or cells with improved northern leaf blight resistance.
INCORPORATION OF SEQUENCE LISTING
[0003] A sequence listing contained in the file named P34361US03_SEQ.txt, which is 217,339 bytes (measure in MS-Windows.RTM.) and created on Nov. 29, 2021, comprising 630 nucleotide sequences, is filed electronically herewith and incorporated by reference in its entirety.
BACKGROUND
[0004] Corn (Zea mays L.) is one of the most important commercial crops in the world. Like many commercial crops, corn is subjected to numerous potentially detrimental environmental conditions (e.g., moisture availability, temperature stresses, soil conditions, pests, disease) that can reduce, or entirely eliminate, crop yield. Crop disease alone accounted for the loss of more than 1.3 billion bushels of corn in the United States and Ontario, Canada in 2012. See Mueller, Corn Disease Loss Estimates from the United States and Ontario, Canada--2012. Purdue Extension Publication BP-96-12-W (2014).
[0005] Northern leaf blight (NLB) is a crop disease caused by the fungal pathogen Exserohilum turcicum (also referred to as Helminthosporium turcicum or Setosphaeria turcica in some literature). NLB can infect corn in tropical and temperate climates. Infected corn plants can exhibit a range of symptoms from cigar-shaped lesions on lower leaves to complete destruction of multiple leaves. Corn infected with NLB is also highly susceptible to stem rot and root rot caused by secondary infections. NLB is particularly problematic in tropical highlands, where environmental conditions favor disease development. However, NLB infection can cause yield losses of 30%-50% in temperate environments, including the United States and Europe.
[0006] E. turcicum overwinters as mycelia and conidia on corn plant parts left on the soil surface. The conidia are transformed into spores, and during warm, wet weather, new conidia are produced and carried by wind or rain onto the lower leaves of corn plants. Infection requires the presence of water on the leaf surface for at least 6 hours and a temperature of between 65.degree. F. and 80.degree. F. If infection occurs, lesions develop within 12 days and produce new conidia which can spread the infection to additional leaves and plants. NLB management strategies include crop rotation, destruction of over-wintering corn plant parts, and fungicide application. However, fungicide application alone is not an efficient mechanism of control, especially in Brazil.
[0007] There is a need in corn breeding to identify corn germplasm that provides resistance to NLB infection. There is also a need to develop polymorphic markers for monitoring and introgressing NLB resistance alleles, and further develop agronomically elite corn lines comprising NLB resistance for enhancing plant productivity.
SUMMARY
[0008] The present disclosure identifies genetic loci conferring NLB resistance in corn, and provides molecular markers linked to these resistance loci. This disclosure further provides methods for introgressing resistance alleles of genetic loci conferring NLB resistance into plant varieties previously lacking such alleles, thereby providing plants with NLB resistance. The genetic loci, markers, and methods provided herein therefore allow for production of new varieties with enhanced NLB resistance.
[0009] In one aspect, this disclosure provides a method of creating a population of corn plants, seeds, or cells comprising genotyping a first population of corn plants, seeds, or cells at one or more marker loci associated with and within about 10 cM of one or more NLB resistance quantitative trait loci (QTLs) selected from the group consisting of NLB resistance QTLs NLB_2.01, NLB_3.01, NLB_4.01, NLB_4.02, NLB_5.01, NLB_6.01, NLB_7.01, and NLB_9.01; selecting from the first population one or more corn plants, seeds, or cells comprising one or more NLB resistance alleles of the one or more marker loci; and producing from the selected one or more corn plants, seeds, or cells a second population of corn plants, seeds, or cells comprising the one or more NLB QTLs.
[0010] In one aspect, this disclosure provides a method of introgressing an NLB resistance QTL comprising crossing a first corn plant comprising an NLB resistance QTL with a second corn plant of a different genotype to produce one or more progeny plants or seeds; and selecting a progeny plant or seed comprising an NLB resistance allele of a polymorphic locus linked to the NLB resistance QTL, where the polymorphic locus is in a chromosomal segment flanked by: any two of marker loci SEQ ID NOs: 1 to 18, any two of marker loci SEQ ID NOs: 19 to 31, any two of marker loci SEQ ID NOs: 32 to 52 and 471-475, any two of marker loci SEQ ID NOs: 53 to 65 and 446 to 468, any two of marker loci SEQ ID NOs: 66 to 84, any two of marker loci SEQ ID NOs: 85 to 89, marker loci SEQ ID NOs: 469 and 470, or any two of marker loci SEQ ID NOs: 476-482.
[0011] In one aspect, this disclosure provides an NLB resistant corn plant, seed, or cell comprising a combination of two or more, three or more, four or more, five or more, six or more, or seven or more introgressed NLB resistance QTLs selected from the group consisting of NLB resistance QTLs NLB_2.01, NLB_3.01, NLB_4.01, NLB_4.02, NLB_5.01, NLB_6.01, NLB_7.01, and NLB_9.01.
[0012] In one aspect, this disclosure provides a method for selecting a corn plant, seed, or cell comprising isolating nucleic acids from a corn plant, seed, or cell; analyzing the nucleic acids to detect a polymorphic marker associated with and within 10 cM of an NLB resistance QTL selected from the group consisting of NLB resistance QTLs NLB_2.01, NLB_3.01, NLB_4.01, NLB_4.02, NLB_5.01, NLB_6.01, NLB_7.01, and NLB_9.01; and selecting a corn plant, seed, or cell comprising the NLB resistance QTL.
[0013] In one aspect, this disclosure provides a method comprising providing a set of corn seeds comprising one or more, two or more, three or more, four or more, five or more, six or more, or seven or more NLB resistance QTLs selected from the group consisting of NLB resistance QTLs NLB_2.01, NLB_3.01, NLB_4.01, NLB_4.02, NLB_5.01, NLB_6.01, NLB_7.01, and NLB_9.01, to a person desirous of planting said set of corn seeds in a field plot.
[0014] In one aspect, this disclosure provides a method of growing a population of corn plants in a field plot, said method comprising planting a population of corn seeds comprising one or more, two or more, three or more, four or more, five or more, six or more, or seven or more introgressed NLB resistance QTLs selected from the group consisting of NLB resistance QTLs NLB_2.01, NLB_3.01, NLB_4.01, NLB_4.02, NLB_5.01, NLB_6.01, NLB_7.01, and NLB_9.01 in said field plot.
BRIEF DESCRIPTION OF THE SEQUENCES
[0015] SEQ ID NOs: 1-89 and 446-482 list sequences of exemplary SNP marker loci associated with an NLB resistance QTL. Example resistant and susceptible alleles of these marker loci are listed in Table 5. SEQ ID NOs: 90-445 and 483-630 list the sequences of exemplary primers and probes which can be used to detect the SNP marker loci of SEQ ID NOs: 1-89 and 446-482.
DETAILED DESCRIPTION
[0016] Unless defined otherwise herein, terms are to be understood according to conventional usage by those of ordinary skill in the relevant art. Examples of resources describing many of the terms related to molecular biology used herein can be found in Alberts et al., Molecular Biology of The Cell, 5.sup.th Edition, Garland Science Publishing, Inc.: New York, 2007; Rieger et al., Glossary of Genetics: Classical and Molecular, 5th edition, Springer-Verlag: New York, 1991; King et al, A Dictionary of Genetics, 6th ed., Oxford University Press: New York, 2002; and Lewin, Genes IX, Oxford University Press: New York, 2007. The nomenclature for DNA bases as set forth at 37 C.F.R. .sctn. 1.822 is used.
[0017] As used herein, terms in the singular and the singular forms "a," "an," and "the," for example, include plural referents unless the content clearly dictates otherwise. Thus, for example, reference to "plant," "the plant," or "a plant" also includes a plurality of plants; also, depending on the context, use of the term "plant" can also include genetically similar or identical progeny of that plant; use of the term "a nucleic acid" optionally includes, as a practical matter, many copies of that nucleic acid molecule; similarly, the term "probe" optionally (and typically) encompasses many similar or identical probe molecules.
[0018] As used herein, "plant" refers to a whole plant and/or progeny of the same. A progeny plant can be from any filial generation, e.g., F.sub.1, F.sub.2, F.sub.3, F.sub.4, F.sub.5, F.sub.6, F.sub.7, etc. A "plant part" refers to any part of a plant, comprising a cell or tissue culture derived from a plant, plant components or organs (e.g., leaves, stems, roots, etc.), plant tissues, seeds, and plant cells. A plant cell is a biological cell of a plant, taken from a plant or derived through culture from a cell taken from a plant.
[0019] As used herein, a "corn plant" or "maize plant" refers to a plant of species Zea mays L and includes all plant varieties that can be bred with corn, including wild maize species.
[0020] As used herein, "germplasm" refers to living sources of genetic material. The germplasm can be part of an organism or cell, or can be separate from the organism or cell. In general, germplasm provides genetic material with a specific molecular makeup that provides a physical foundation for some or all of the hereditary qualities of an organism or cell culture. As used herein, germplasm includes cells, seed, or tissues from which new plants can be grown, or plant parts, such as leaves, stems, pollen, or cells that can be cultured into a whole plant.
[0021] As used herein, the phrase "associated with" or "linked to" refers to a recognizable and/or assayable relationship between two entities. For example, the phrase "associated with NLB resistance" refers to a trait, locus, gene, allele, marker, phenotype, etc., or the expression thereof, the presence or absence of which can influence an extent, degree, and/or rate at which a plant or a part of interest thereof that has an NLB resistance trait. As such, a marker is "associated with" a trait when it is linked to it and when the presence of the marker is an indicator of whether and/or to what extent the desired trait or trait form will occur in a plant/germplasm comprising the marker. Similarly, a marker is "associated with" an allele when it is linked to it and when the presence of the marker is an indicator of whether the allele is present in a plant/germplasm comprising the marker. For example, "a marker associated with a resistance allele" refers to a marker whose presence or absence can be used to predict whether and to what extent a plant will display an NLB resistance phenotype.
[0022] As used herein, a "centimorgan" (cM) is a unit of measure of recombination frequency and genetic distance between two loci. One cM is equal to a 1% chance that a marker at one genetic locus will be separated from a marker at a second locus due to crossing over in a single generation.
[0023] As used herein, "closely linked" means that the marker or locus is within about 20 cM, 15 cM, 10 cM, 5 cM, 4 cM, 3 cM, 2 cM, 1 cM, 0.5 cM, or less than 0.5 cM of another marker or locus. For example, 20 cM means that recombination occurs between the marker and the locus with a frequency of equal to or less than about 20%.
[0024] As used herein, "locus" is a chromosome region or chromosomal region where a polymorphic nucleic acid, trait determinant, gene, or marker is located. A locus can represent a single nucleotide, a few nucleotides or a large number of nucleotides in a genomic region. The loci of this disclosure comprise one or more polymorphisms in a population (e.g., alternative alleles are present in some individuals).
[0025] As used herein, "allele" refers to an alternative nucleic acid sequence at a particular locus. The length of an allele can be as small as one nucleotide base. For example, a first allele can occur on one chromosome, while a second allele occurs on a second homologous chromosome, e.g., as occurs for different chromosomes of a heterozygous individual, or between different homozygous or heterozygous individuals in a population.
[0026] As used herein, "crossed," "cross," or "crossing" means to produce progeny via fertilization (e.g., cells, seeds, or plants) and includes crosses between plants (sexual) and self-fertilization (selfing).
[0027] As used herein, "backcross" and "backcrossing" refer to the process whereby a progeny plant is repeatedly crossed back to one of its parents. In a backcrossing scheme, the "donor" parent refers to the parental plant with the desired gene or locus to be introgressed. The "recipient" parent (used one or more times) or "recurrent" parent (used two or more times) refers to the parental plant into which the gene or locus is being introgressed. For example, see Ragot et al., Marker-assisted Backcrossing: A Practical Example, in Techniques Et Utilisations Des Marqueurs Moleculaires Les Colloques, 72:45-56 (1995); and Openshaw et al., Marker-assisted Selection in Backcross Breeding, in Proceedings Of The Symposium "Analysis Of Molecular Marker Data," pp. 41-43 (1994). The initial cross gives rise to the F.sub.1 generation. The term "BC1" refers to the second use of the recurrent parent, "BC2" refers to the third use of the recurrent parent, and so on. In an aspect, a backcross is performed repeatedly, with a progeny individual of each successive backcross generation being itself backcrossed to the same parental genotype.
[0028] As used herein, "agronomically elite background" means any line that has resulted from breeding and selection for superior agronomic performance. Similarly, an "elite germplasm" or elite strain of germplasm is an agronomically superior germplasm. Numerous elite lines are available and known to those of skill in the art of corn breeding.
[0029] As used herein, "genotype" is the genetic constitution of an individual (or group of individuals) at one or more genetic loci, as contrasted with the observable trait (phenotype). Genotype is defined by the allele(s) of one or more known loci that the individual has inherited from its parents. The term genotype can be used to refer to an individual's genetic constitution at a single locus, at multiple loci, or, more generally, the term genotype can be used to refer to an individual's genetic make-up for all the genes in its genome. The term "genotype" can also refer to determining the genetic constitution of an individual (or group of individuals) at one or more genetic loci.
[0030] As used herein, a "haplotype" is the genotype of an individual at a plurality of genetic loci. Typically, the genetic loci described by a haplotype are physically and genetically linked, e.g., in the same chromosome interval. A haplotype can also refer to a combination of SNP alleles located within a single gene.
[0031] As used herein, "marker assay" means a method for detecting a polymorphism at a particular locus using a particular method, e.g. measurement of at least one phenotype (such as seed color, flower color, or other visually detectable traits), restriction fragment length polymorphism (RFLP), single base extension, electrophoresis, sequence alignment, allelic specific oligonucleotide hybridization (ASO), random amplified polymorphic DNA (RAPD), microarray-based technologies, and nucleic acid sequencing technologies, etc.
[0032] As used herein, "marker assisted selection" (MAS) is a process by which phenotypes are selected based on marker genotypes. "Marker assisted selection breeding" refers to the process of selecting a desired trait or traits in a plant or plants by detecting one or more nucleic acids from the plant, where the nucleic acid is linked to the desired trait, and then selecting the plant or germplasm possessing those one or more nucleic acids.
[0033] As used herein, "polymorphism" means the presence of one or more variations in a population. A polymorphism can manifest as a variation in the nucleotide sequence of a nucleic acid or as a variation in the amino acid sequence of a protein. Polymorphisms include the presence of one or more variations of a nucleic acid sequence or nucleic acid feature at one or more loci in a population of one or more individuals. The variation can comprise, but is not limited to, one or more nucleotide base changes, the insertion of one or more nucleotides or the deletion of one or more nucleotides. A polymorphism can arise from random processes in nucleic acid replication, through mutagenesis, as a result of mobile genomic elements, from copy number variation and during the process of meiosis, such as unequal crossing over, genome duplication, and chromosome breaks and fusions. The variation can be commonly found or can exist at low frequency within a population, the former having greater utility in general plant breeding and the latter can be associated with rare but important phenotypic variation. Useful polymorphisms can include a single nucleotide polymorphisms (SNP), an insertion or deletion in DNA sequence (indel), a simple sequence repeats of DNA sequence (SSR), a restriction fragment length polymorphism (RFLP), and a tag SNP. A genetic marker, a gene, a DNA-derived sequence, a RNA-derived sequence, a promoter, a 5' untranslated region of a gene, a 3' untranslated region of a gene, microRNA, small interfering RNA, a tolerance locus, a satellite marker, a transgene, mRNA, double-stranded RNA, a transcriptional profile, and a methylation pattern can also comprise a polymorphism. In addition, the presence, absence, or variation in copy number of the preceding can comprise a polymorphism.
[0034] As used herein, "SNP" or "single nucleotide polymorphism" means a sequence variation that occurs when a single nucleotide (A, T, C, or G) in the genome sequence is altered or variable. "SNP markers" exist when SNPs are mapped to sites on the genome.
[0035] As used herein, "marker," "molecular marker," or "marker locus" is a term used to denote a nucleic acid or amino acid sequence that is sufficiently unique to characterize a specific locus on the genome. Any detectable polymorphic trait can be used as a marker so long as it is inherited differentially and exhibits linkage disequilibrium with a phenotypic trait of interest. A number of markers and integrated genetic maps have been developed for corn (e.g., the UMC 98 map, the Nested Association Mapping (NAM) map, the Intermated B73/Mol7 (IBM2) Neighbors 2008 genetic map, and the LHRF Gnp2004 map. See maizegdb.org/data_center/map for more). All markers are used to define a specific locus in corn genomes. Large numbers of these markers have been mapped. See maizegdb.org/data_center/marker. Each marker is therefore an indicator of a specific segment of DNA, having a unique nucleotide sequence. The map positions provide a measure of the relative positions of particular markers with respect to one another. When a trait is stated to be linked to a given marker it will be understood that the actual DNA segment whose sequence affects the trait generally co-segregates with the marker. More precise and definite localization of a trait can be obtained if markers are identified on both sides of the trait. By measuring the appearance of the marker(s) in progeny of crosses, the existence of the trait can be detected by relatively simple molecular tests without actually evaluating the appearance of the trait itself, which can be difficult and time-consuming because the actual evaluation of the trait requires growing plants to a stage and/or under environmental conditions where the trait can be expressed. Molecular markers have been widely used to determine genetic composition in corn. In an aspect, markers used herein exhibit LOD scores of 2 or greater, 3 or greater, 4 or greater, 5 or greater, 6 or greater, 7 or greater, 8 or greater, or 9 or greater with an associated trait of interest (e.g., NLB resistance), measuring using a method known in the art such as Qgene Version 2.23 (1996) and default parameters. Without being limiting, examples of molecular markers and molecular marker systems include SNPs, indels, RFLPs, SSRs, restriction site-associated DNA (RAD), diversity array technology (DArT), and genotyping by sequencing (GBS).
[0036] As used herein, "linkage disequilibrium" (LD) refers to a non-random segregation of genetic loci or traits (or both). In either case, linkage disequilibrium implies that the relevant loci are within sufficient physical proximity along a length of a chromosome so that they segregate together with greater than random (i.e., non-random) frequency (in the case of co-segregating traits, the loci that underlie the traits are in sufficient proximity to each other). Linked loci co-segregate more than 50% of the time, e.g., from about 51% to about 100% of the time. Linkage disequilibrium can be measured using any one of the methods provided in Hedrick, Gametic disequilibrium measures: proceed with caution. Genetics, 117:331-41(1987). The term "physically linked" is sometimes used to indicate that two loci, e.g., two marker loci, are physically present on the same chromosome. Advantageously, the two linked loci are located in close proximity such that recombination between homologous chromosome pairs does not occur between the two loci during meiosis with high frequency, e.g., such that linked loci co-segregate at least about 90% of the time, e.g., 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 99.5%, 99.75%, or more of the time.
[0037] As used herein, a "genetic map" is the relationship of genetic linkage among loci on one or more chromosomes (or linkage groups) within a given species, generally depicted in a diagrammatic or tabular form. Genetic mapping is the process of defining the linkage relationships of loci through the use of genetic markers, populations segregating for the markers, and standard genetic principles of recombination frequency. A genetic map location is a location on a genetic map relative to surrounding genetic markers on the same linkage group where a specified marker can be found within a given species. In contrast, a "physical map" of the genome refers to absolute distances (for example, measured in base pairs or isolated and overlapping contiguous genetic fragments, e.g., contigs). In general, the closer two markers or genomic loci are on the genetic map, the closer they lie to one another on the physical map. A physical map of the genome does not take into account the genetic behavior (e.g., recombination frequencies) between different points on the physical map. A lack of precise proportionality between genetic distances and physical distances can exist due to the fact that the likelihood of genetic recombination is not uniform throughout the genome; some chromosome regions are cross-over "hot spots," while other regions demonstrate only rare recombination events, if any. Genetic mapping variability can also be observed between different populations of the same crop species. In spite of this variability in the genetic map that can occur between populations, genetic map and marker information derived from one population generally remains useful across multiple populations in identification of plants with desired traits, counter-selection of plants with undesirable traits and in MAS breeding. As one of skill in the art will recognize, recombination frequencies (and as a result, genetic map positions) in any particular population are not static. The genetic distances separating two markers (or a marker and a QTL) can vary depending on how the map positions are determined. For example, variables such as the parental mapping populations used, the software used in the marker mapping or QTL mapping, and the parameters input by the user of the mapping software can contribute to the QTL marker genetic map relationships. However, it is not intended that this disclosure be limited to any particular mapping populations, use of any particular software, or any particular set of software parameters to determine linkage of a particular marker or haplotypes with a desired phenotype. It is well within the ability of one of ordinary skill in the art to extrapolate the novel features described herein to any gene pool or population of interest, and using any particular software and software parameters. Indeed, observations regarding genetic markers and haplotypes in populations in addition to those described herein are readily made using the teaching of the present disclosure.
[0038] As used herein, "selecting" or "selection" in the context of marker-assisted selection or breeding refer to the act of picking or choosing desired individuals, normally from a population, based on certain pre-determined criteria.
[0039] As used herein, "primer" refers to an oligonucleotide (synthetic or occurring naturally), which is capable of acting as a point of initiation of nucleic acid synthesis or replication along a complementary strand when placed under conditions in which synthesis of a complementary strand is catalyzed by a polymerase. Typically, primers are about 10 to 30 nucleotides in length, but longer or shorter sequences can be employed. Primers can be provided in double-stranded form, though the single-stranded form is more typically used. A primer can further contain a detectable label (e.g., a 5' end label).
[0040] As used herein, "probe" refers to an oligonucleotide (synthetic or occurring naturally) that is complementary (though not necessarily fully complementary) to a polynucleotide of interest and forms a duplex structure by hybridization with at least one strand of the polynucleotide of interest. Typically, probes are oligonucleotides from 10 to 50 nucleotides in length, but longer or shorter sequences can be employed. A probe can further contain a detectable label.
[0041] As used herein, a "population of plants" or a "population of seeds" means a set comprising any number, at least two, of individuals, objects, or data from which samples are taken for evaluation. Most commonly, the terms relate to a breeding population of plants from which members are selected and crossed to produce progeny in a breeding program. A population of plants can include the progeny of a single breeding cross or a plurality of breeding crosses, and can be either actual plants or plant derived material, or in silico representations of the plants or seeds. The population members need not be identical to the population members selected for use in subsequent cycles of analyses or those ultimately selected to obtain final progeny plants or seeds. Often, a population of plants or seeds is derived from a single biparental cross, but can also derive from two or more crosses between the same or different parents. Although a population of plants or seeds can comprise any number of individuals, those of skill in the art will recognize that plant breeders commonly use population sizes ranging from one or two hundred individuals to several thousand, and that the highest performing 5% to 20% of a population is what is commonly selected to be used in subsequent crosses in order to improve the performance of subsequent generations of the population.
[0042] As used herein, "cultivar" and "variety" are used synonymously and mean a group of plants within a species (e.g., Z. mays L.) that share certain genetic traits that separate them from other possible varieties within that species. Corn cultivars can be inbreds or hybrids, though commercial corn cultivars are mostly hybrids to take advantage of hybrid vigor. Individuals within a corn hybrid cultivar are homogeneous, nearly genetically identical, with most loci in the heterozygous state.
[0043] As used herein, the term "inbred" means a line that has been bred for genetic homogeneity.
[0044] As used herein, the term "hybrid" means a progeny of mating between at least two genetically dissimilar parents. Without limitation, examples of mating schemes include single crosses, modified single cross, double modified single cross, three-way cross, modified three-way cross, and double cross wherein at least one parent in a modified cross is the progeny of a cross between sister lines.
[0045] As used herein, "introgression" refers to the transmission of a desired allele of a genetic locus from one genetic background to another.
[0046] As used herein, the term "chromosome interval" or "chromosomal interval" designates a contiguous linear span of genomic DNA that resides on a single chromosome.
[0047] As used herein, "flanked by," when used to describe a chromosomal interval, refers to two loci physically surrounding the chromosomal interval, with one locus on each side of the chromosomal interval. As referenced herein, a chromosomal interval flanked by two marker loci includes the two marker loci.
[0048] As used herein, a "resistant allele" or "resistance allele" is an allele at a particular locus that confers, or contributes to, NLB resistance, or alternatively, is an allele that allows the identification of plants that comprise NLB resistance. A resistant allele of a marker is a marker allele that segregates with NLB resistance, or alternatively, segregates with NLB susceptibility, therefore providing the benefit of identifying plants having NLB susceptibility. A resistant allelic form of a chromosome interval is a chromosome interval that includes a nucleotide sequence that contributes to NLB resistance at one or more genetic loci physically located in the chromosome interval.
[0049] As used herein, "genetic element" or "gene" refers to a heritable sequence of DNA, e.g., a genomic sequence, with functional significance. The term "gene" can also be used to refer to, e.g., a cDNA and/or an mRNA encoded by a genomic sequence, as well as to that genomic sequence.
[0050] As used herein, the terms "phenotype," or "phenotypic trait," or "trait" refers to one or more detectable characteristics of a cell or organism which can be influenced by genotype. The phenotype can be observable to the naked eye, or by any other means of evaluation known in the art, e.g., microscopy, biochemical analysis, genomic analysis, an assay for a particular disease tolerance, etc. In some cases, a phenotype is directly controlled by a single gene or genetic locus, e.g., a "single gene trait." In other cases, a phenotype is the result of several genes.
[0051] As used herein, "resistance" and "enhanced resistance" are used interchangeably herein and refer to any type of increase in resistance, or any type of decrease in susceptibility. A plant or plant variety exhibiting resistance need not possess absolute or complete resistance. Instead, a plant or plant variety with "enhanced resistance" will have a level of resistance which is higher than that of a comparable susceptible plant or variety. The level of NLB resistance can be determined based on disease ratings as determined in Example 1. Briefly, resistance to NLB infection of corn plants is scored using an NLB resistance scale, wherein NLB resistance is measured by rating the percentage of leaf area infected on a scale of 1 to 9. An NLB resistance scale comprises ratings of: 1 (highly resistant; 0% of leaf area infected, no visible lesions), 2 (highly resistant; less than 1% leaf area infected, few lesions dispersed through lower leaves), 3 (resistant; 1% to less than 20% leaf area infected), 4 (resistant; 20% to less than 40% leaf area infected), 5 (mildly resistant; 40% to less than 50% leaf area infected, lesions reaching ear leaf with sparse lesions in leaves above the ear), 6 (mildly susceptible; 50% to less than 60% leaf area infected, lesions reaching the leaves above the ears), 7 (susceptible; 60% to less than 75% leaf area infected), 8 (susceptible; 75% to less than 90% leaf area infected), and 9 (susceptible; greater than 90% of leaf area infected, with premature death of the plant).
[0052] As used herein, "quantitative trait locus" (QTL) or "quantitative trait loci" (QTLs) refer to a genetic domain that effects a phenotype that can be described in quantitative terms and can be assigned a "phenotypic value" which corresponds to a quantitative value for the phenotypic trait.
[0053] As used herein, "adjacent", when used to describe a nucleic acid molecule that hybridizes to DNA containing a polymorphism, refers to a nucleic acid that hybridizes to DNA sequences that directly abut the polymorphic nucleotide base position. For example, a nucleic acid molecule that can be used in a single base extension assay is "adjacent" to the polymorphism.
[0054] As used herein, "northern leaf blight" or "NLB" refers to a plant disease caused by the fungal pathogen Exserohilum turcicum, which is also referred to as Helminthosporium turcicum or Setosphaeria turcica.
[0055] As used herein, "field plot" refers to a location that is suitable for growing corn. The location can be indoors (e.g., a greenhouse or a growth chamber) or outdoors; irrigated or non-irrigated; in the ground or in a container that holds soil.
[0056] As used herein, a "planting season" is the length of time, typically about 90-120 days, in which corn can be grown from seed to maturity. One skilled in the art would recognize that a "planting season" could be significantly shorter or longer than about 90-120 days depending on the corn variety being grown and environmental conditions.
[0057] As used herein, "transgenic" means a plant or seed whose genome has been altered by the stable integration of recombinant DNA. A transgenic line includes a plant regenerated from an originally-transformed plant cell and progeny transgenic plants from later generations or crosses of a transformed plant.
[0058] As used herein, "haploid" means a line that has had its normal chromosome complement reduced by half, typically by pollinating an ear with pollen from a haploid inducing line. In corn, haploid refers to an individual plant or seed that has a haploid chromosome complement where n=10, instead of the normal diploid chromosome complement where 2n=20. A "doubled haploid" refers to a haploid line (n=10) that has been induced, typically via chemical means, to double its chromosome complement and return to a diploid state (2n=20) that is homozygous at all loci within the genome.
[0059] As used herein, "yield penalty" refers to a reduction of seed yield in a line correlated with or caused by the presence of an NLB resistance allele or NLB resistance QTL as compared to a line that does not contain that NLB resistance allele or NLB resistance QTL.
[0060] As used herein, "seed yield" can refer to a measure of crop production such as test weight, seed number per plant, seed weight, seed number per unit area (i.e. seeds, or weight of seeds, per acre), bushels per acre, tons per acre, kilograms per hectare, or quintals per hectare.
[0061] Northern leaf blight is a plant disease caused by the fungal pathogen Exserohilum turcicum (also referred to as Helminthosporium turcicum or Setosphaeria turcica by some researchers). NLB afflicts corn in temperate and tropical regions worldwide. NLB is endemic to the United States. Yield losses of over 30% are reported in susceptible hybrids, and yield loss can reach 70% if the onset of NLB infection occurs 2-3 weeks after silking. See Perkins and Pedersen, Disease development and yield losses associated with northern leaf blight on corn, Plant Disease, 71: 940-943 (1987); and Pataky, Relationships between yield of sweet corn and northern leaf blight caused by Exserohilum turcicum, Phytopathology, 82: 370-375 (1992).
[0062] Corn plants in tropical regions are especially at risk to NLB infection due to environmental conditions that are conducive to NLB growth. E. turcicum thrives in humid environments with heavy dews, frequent rain showers, and moderate temperatures. However, E. turcicum also overwinters in areas that see hard freezes as mycelia and conidia on corn plant parts left on the soil surface and NLB is also a major corn disease in temperate regions. NLB conidia are transformed into resting spores during warm, wet weather in spring and early summer. New conidia are then produced and carried by wind or rain onto the lower leaves of young corn plants. Infection requires the presence of water on the leaf surface for 6-18 hours and a temperature of between 65.degree. F. and 80.degree. F. If infection occurs, lesions develop within 12 days and produce new conidia which can spread the infection to additional leaves and plants via new spores carried by wind or rain. NLB lesions can begin producing spores in as little as 7 days under ideal conditions. Generally, NLB infections begin on lower leaves and progress upwards to younger leaves. However, during high spore loads infections can begin at the top of the plant, including the tassel and flag leaf.
[0063] The first sign of NLB infection is an elliptical, or cigar-shaped, gray-green lesion. Lesions typically, but not always, occur on lower leaves before upper leaves. As lesions enlarge they can reach over 6 inches in length and turn a pale gray to brown in color. NLB lesions are not restricted by leaf veins, and the entire leaf can be covered by one or a few lesions in an advanced infection as individual lesions grow and merge. Leaves from highly susceptible plants often appear gray or burned, with little or no healthy, green, photosynthetic tissue remaining. The reduction in tissue capable of photosynthesis leads to a lack of carbohydrates needed for grain fill, which can reduce seed yield. During moist conditions lesions, especially on a lower leaf surface, can produce numerous dark gray spores. Plants infected with NLB are also susceptible to secondary infections from fungi, bacteria, and/or viruses that can cause stem rot and/or root rot.
[0064] Several fungicides, including picoxystrobin, cyproconazole, tetraconazole, pyraclostrobin, metconazole, azoxystrobin, propiconazole, prothioconazole, trifloxystrobin, and combinations thereof are used to treat NLB. However, reliance on chemical agents to reduce NLB incidence is unreliable because NLB can develop resistance to the chemical agents.
[0065] Four NLB resistance loci conferring incomplete, race-specific, NLB resistance have been reported in corn: Ht1, Ht2, Ht3, and Htn1. Resistance conferred by these loci appears dependent on environmental conditions (e.g., light, temperature), and the loci tend to confer delayed leaf lesion development or delayed sporulation rather than complete disease resistance.
[0066] Ht1 maps to the long arm of chromosome 1. See Bentolila et al., Identification of an RFLP marker tightly linked to the Ht1 gene in maize. Theoretical and Applied Genetics. 82: 393-398 (1991). Ht2 maps to the long arm of chromosome 8, and Htn1 maps approximately 10 centimorgans distal to Ht2 on chromosome 8. See Zaitlin et al., Linkage of a second gene for NCLB resistance to molecular markers in maize. Maize Genetics Cooperative Newsletter. 66: 69-70 (1992); Simcox and Bennetzen, The use of molecular markers to study Setosphaeria turcica resistance in maize. Phytopathology. 83: 1326-1330 (1993); Yin et al., Fine mapping of the Ht2 (Helminthosporium turcicum resistance 2) gene in maize. Chinese Science Bulletin. 48: 165-169 (2003); and Chung et al., Characterization and fine-mapping of a resistance locus for northern leaf blight in maize bin 8.06. Theoretical and Applied Genetics. 121: 205-227 (2010). Ht3 maps to chromosome 7. See Van Staden et al., SCAR markers for the Ht1, Ht2, Ht3, and Htn1 resistance genes in maize. Maize Genetics Conference Abstract. 43: P134 (2001).
[0067] A corn plant, seed, or cell provided herein possesses one or more NLB resistance QTLs and/or one or more NLB resistance alleles that confer enhanced resistance to NLB compared to a corn plant, seed, or cell that lacks the one or more NLB resistance QTLs and/or one or more NLB resistance alleles. Further, a corn plant, seed, or cell provided herein provided herein does not suffer a yield penalty when grown in the absence of NLB spores, conidia, and/or mycelia.
[0068] In an aspect, a corn plant, seed, or cell provided herein is a Zea mays L. corn plant, seed, or cell. In another aspect, a corn plant, seed, or cell provided herein is a Zea mays ssp. mays corn plant, seed, or cell. In yet another aspect, a corn plant or seed provided herein is a domesticated line, cultivar, or variety of corn plant or seed. In another aspect, a corn plant, seed, or cell provided herein is a sweet corn plant, sweet corn seed, or sweet corn cell.
[0069] In an aspect, this disclosure provides quantitative trait loci (QTLs) that exhibit significant co-segregation with NLB resistance. The QTLs of this disclosure can be tracked during plant breeding or introgressed into a desired genetic background in order to provide plants exhibiting enhanced NLB resistance and one or more other beneficial traits. As an example, this disclosure identifies QTL intervals that are associated with NLB resistance in corn varieties CV114258, CV115214, CV099829, CV102084, CV095508, CV103141, CV105893, CV595358, CV593417, CV117407, CV592505, and CV592420.
[0070] In an aspect, this disclosure provides molecular markers closely linked to one or more NLB resistance QTLs and methods of using these markers for detection of and selection for NLB resistance. An aspect of this disclosure includes specific markers and their resistance alleles, chromosome intervals comprising the markers, and methods of detecting markers genetically linked to NLB resistance to identify plant lines with enhanced NLB resistance. For example, one aspect of this disclosure provides a chromosome interval associated with NLB resistance which is flanked by any two of marker loci SEQ ID NOs: 12 to 15. Another example of this disclosure provides a chromosome interval associated with NLB resistance, where the interval is flanked by any two of marker loci SEQ ID NOs: 22 to 25. Another example of this disclosure provides a chromosome interval associated with NLB resistance which is flanked by any two of marker loci SEQ ID NOs: 37 to 42 and 474. Another example of this disclosure provides a chromosome interval associated with NLB resistance which is flanked by any two of marker loci SEQ ID NOs: 44 to 49. Another example of this disclosure provides a chromosome interval associated with NLB resistance which is flanked by any two of marker loci SEQ ID NOs: 57 to 62 and 458 to 466. Another example of this disclosure provides a chromosome interval associated with NLB resistance which is flanked by any two of marker loci SEQ ID NOs: 79 to 81. Another example of this disclosure provides a chromosome interval associated with NLB resistance which is flanked by any two of marker loci SEQ ID NOs: 87 to 89 and 477 to 480. Another example of this disclosure provides a chromosome interval associated with NLB resistance which is flanked by marker loci SEQ ID NOs: 469 and 470.
[0071] One aspect of this disclosure provides a chromosome interval associated with NLB resistance which is flanked by any two of marker loci SEQ ID NOs: 8 to 18. Another aspect of this disclosure provides a chromosome interval associated with NLB resistance, where the interval is flanked by any two of marker loci SEQ ID NOs: 21 to 29. Another aspect of this disclosure provides a chromosome interval associated with NLB resistance which is flanked by any two of marker loci SEQ ID NOs: 33 to 42, 473, and 474. Another aspect of this disclosure provides a chromosome interval associated with NLB resistance which is flanked by any two of marker loci SEQ ID NOs: 43 to 49 and 475. Another aspect of this disclosure provides a chromosome interval associated with NLB resistance which is flanked by any two of marker loci SEQ ID NOs: 57 to 64 and 458 to 468. Another aspect of this disclosure provides a chromosome interval associated with NLB resistance which is flanked by any two of marker loci SEQ ID NOs: 74 to 82. Another aspect of this disclosure provides a chromosome interval associated with NLB resistance which is flanked by any two of marker loci SEQ ID NOs: 86 to 89 and 476, 477, 479, and 480. Also provided herein are markers, e.g., SEQ ID NOs: 1-89 and 446-482, that are useful for tracking NLB resistant alleles and can be used in MAS breeding programs to produce plants with enhanced NLB resistance.
[0072] This disclosure further provides methods of using the markers identified herein to introgress loci associated with NLB resistance into NLB susceptible plants. As an example, one skilled in the art can use this disclosure to create a novel corn plant, seed, or cell with NLB resistance by crossing a donor line comprising a QTL provided herein with any desired recipient line, with or without MAS.
[0073] In another aspect, this disclosure further provides methods for introgressing multiple NLB resistance QTLs identified herein to generate an enhanced NLB resistant population of corn plants, seeds, or cells.
[0074] In an aspect, this disclosure provides a method of creating a population of corn plants, seeds, or cells, where the method comprises the steps of: (a) genotyping a first population of corn plants, seeds, or cells at one or more marker loci associated with one or more NLB resistance QTLs selected from the group consisting of NLB resistance QTLs NLB_2.01, NLB_3.01, NLB_4.01, NLB_4.02, NLB_5.01, NLB_6.01, NLB_7.01, and NLB_9.01; (b) selecting from the first population one or more corn plants, seeds, or cells comprising one or more NLB resistance alleles of the one or more marker loci; and (c) producing from the selected one or more corn plants, seeds, or cells a second population of corn plants, seeds, or cells comprising one or more NLB QTLs.
[0075] In an aspect, this disclosure provides a corn plant, seed, or cell as described in any of paragraphs [00175] to [00183], where the corn seed further comprises one or more NLB resistance loci selected from the group consisting of Ht1, Ht2, Ht3, and Htn1. In another aspect, this disclosure provides a corn plant, seed, or cell as described in any of paragraphs [00175] to [00183], where the corn seed further comprises two or more NLB resistance loci selected from the group consisting of Ht1, Ht2, Ht2, and Htn1. In another aspect, this disclosure provides a corn plant, seed, or cell as described in any of paragraphs [00175] to [00183], where the corn seed further comprises three or more NLB resistance loci selected from the group consisting of Ht1, Ht2, Ht2, and Htn1. In another aspect, this disclosure provides a corn plant, seed, or cell as described in any of paragraphs [00175] to [00183], where the corn seed further comprises NLB resistance loci Ht1, Ht2, Ht2, and Htn1.
[0076] In another aspect, this disclosure provides a corn plant, seed, or cell comprising a first NLB resistance locus selected from the group consisting of NLB resistance QTLs NLB_2.01, NLB_3.01, NLB_4.01, NLB_4.02, NLB_5.01, NLB_6.01, NLB_7.01, and NLB_9.01 and a second NLB resistance locus Ht1. In another aspect, this disclosure provides a corn plant, seed, or cell comprising a first NLB resistance locus selected from the group consisting of NLB resistance QTLs NLB_2.01, NLB_3.01, NLB_4.01, NLB_4.02, NLB_5.01, NLB_6.01, NLB_7.01, and NLB_9.01 and a second NLB resistance locus Ht2. In another aspect, this disclosure provides a corn plant, seed, or cell comprising a first NLB resistance locus selected from the group consisting of NLB resistance QTLs NLB_2.01, NLB_3.01, NLB_4.01, NLB_4.02, NLB_5.01, NLB_6.01, NLB_7.01, and NLB_9.01 and a second NLB resistance locus Ht3. In another aspect, this disclosure provides a corn plant, seed, or cell comprising a first NLB resistance locus selected from the group consisting of NLB resistance QTLs NLB_2.01, NLB_3.01, NLB_4.01, NLB_4.02, NLB_5.01, NLB_6.01, NLB_7.01, and NLB_9.01 and a second NLB resistance locus Htn1.
[0077] In another aspect, this disclosure provides a corn plant, seed, or cell comprising a first NLB resistance locus selected from the group consisting of NLB resistance QTLs NLB_4.01 and NLB_4.02 and a second NLB resistance locus selected from the group consisting of Ht1, Ht2, Ht3, and Htn1. In another aspect, this disclosure provides a corn plant, seed, or cell comprising a first NLB resistance locus selected from the group consisting of NLB resistance QTLs NLB_4.01 and NLB_4.02 and a second NLB resistance locus Ht1. In another aspect, this disclosure provides a corn plant, seed, or cell comprising a first NLB resistance locus selected from the group consisting of NLB resistance QTLs NLB_4.01 and NLB_4.02 and a second NLB resistance locus Ht2. In another aspect, this disclosure provides a corn plant, seed, or cell comprising a first NLB resistance locus selected from the group consisting of NLB resistance QTLs NLB_4.01 and NLB_4.02 and a second NLB resistance locus Ht3. In another aspect, this disclosure provides a corn plant, seed, or cell comprising a first NLB resistance locus selected from the group consisting of NLB resistance QTLs NLB_4.01 and NLB_4.02 and a second NLB resistance locus Htn1.
[0078] In an aspect, this disclosure provides a method of creating a population of corn plants, seeds, or cells, which method comprising the steps of: (a) genotyping a first population of corn plants, the population comprising at least one allele associated with NLB resistance, wherein the NLB resistance allele is associated with a marker selected from the group consisting of SEQ ID NOs: 1-89 and 446-482; (b) selecting from the first population one or more corn plants, seeds, or cells comprising the NLB resistance allele; and (c) producing from the selected corn plants, seeds, or cells a second population of corn plants, seeds, or cells comprising the at least one NLB resistance allele.
[0079] In an aspect, this disclosure provides a method for introgressing a resistance allele of a locus conferring NLB resistance, which method comprising the steps of: (a) crossing a first corn plant with a second corn plant, wherein the first corn plant comprises the resistance allele, wherein the NLB resistance allele is associated with a marker selected from the group consisting of SEQ ID NOs: 1-89 and 446-482; (b) genotyping a progeny corn plant or seed from the cross using a marker associated with the resistance allele; and (c) selecting a progeny plant or seed comprising the resistance allele.
[0080] In an aspect, this disclosure provides a method for introgressing an NLB resistance QTL, which method comprising the steps of: (a) crossing a first corn plant comprising an NLB resistance QTL selected from the group consisting of NLB resistance QTLs NLB_2.01, NLB_3.01, NLB_4.01, NLB_4.02, NLB_5.01, NLB_6.01, NLB_7.01, and NLB_9.01, with a second corn plant of a different genotype to produce one or more progeny plants or seeds; (b) assaying the one or more progeny plants or seeds at a marker locus associated with the NLB resistance QTL; and (c) selecting a progeny plant or seed comprising the NLB resistance QTL.
[0081] In an aspect, this disclosure provides a method for creating a population of corn plants, seeds, or cells with NLB resistance, which method comprising the steps of: (a) concurrently detecting in a first population of corn plants, seeds, or cells the presence of a combination of two or more, three or more, four or more, five or more, six or more, or seven or more introgressed NLB resistance loci selected from the group consisting of NLB resistance QTLs NLB_2.01, NLB_3.01, NLB_4.01, NLB_4.02, NLB_5.01, NLB_6.01, NLB_7.01, and NLB_9.01; (b) selecting from the first population one or more corn plants or seed comprising the one or more, two or more, three or more, four or more, five or more, six or more, or seven or more introgressed NLB resistance QTLs; and (c) producing a population of offspring from the selected one or more corn plants, seeds, or cells. In an aspect, a method comprises concurrent detection of one or more molecular markers located in at least one chromosome interval flanked by any two of marker loci SEQ ID NOs: 1 to 18, any two of marker loci SEQ ID NOs: 19 to 31, any two of marker loci SEQ ID NOs: 32 to 52 and 471 to 475, any two of marker loci SEQ ID NOs: 53 to 65 and 446 to 468, any two of marker loci SEQ ID NOs: 66 to 84, any two of marker loci SEQ ID NOs: 85 to 89 and 476 to 482, or marker loci SEQ ID NOs: 469 and 470. In another aspect, a method comprises concurrent detection of one or more molecular markers located in at least one chromosome interval flanked by any two of marker loci SEQ ID NOs: 12 to 15, any two of marker loci SEQ ID NOs: 22 to 25, any two of marker loci SEQ ID NOs: 37 to 42 and 474, any two of marker loci SEQ ID NOs: 44 to 49, any two of marker loci SEQ ID NOs: 57 to 62 and 458 to 466, any two of marker loci SEQ ID NOs: 79 to 81, or any two of marker loci SEQ ID NOs: 87 to 89 and 477 to 480. In another aspect, a method comprises concurrent detection of one or more molecular markers located in at least one chromosome interval flanked by any two of marker loci SEQ ID NOs: 8 to 18, any two of marker loci SEQ ID NOs: 21 to 29, any two of marker loci SEQ ID NOs: 33 to 42, 473, and 474, any two of marker loci SEQ ID NOs: 43 to 49 and 475, any two of marker loci SEQ ID NOs: 57 to 64 and 458 to 468, any two of marker loci SEQ ID NOs: 74 to 82, or any two of marker loci SEQ ID NOs: 86 to 89 and 476, 477, 479, and 480.
[0082] In an aspect, a method comprises concurrently detecting NLB resistance QTLs NLB_4.01, and NLB_4.02. In another aspect, a method comprises concurrently detecting NLB resistance QTLs NLB_4.01 and NLB_4.02 and at least one or more NLB resistance QTLs selected from the group consisting of NLB_2.01, NLB_3.01, NLB_5.01, NLB_6.01, NLB_7.01, and NLB_9.01. In another aspect, a method comprises concurrently detecting NLB resistance QTLs NLB_4.01 and NLB_4.02 and at least two or more NLB resistance QTLs selected from the group consisting of NLB_2.01, NLB_3.01, NLB_5.01, NLB_6.01, NLB_7.01, and NLB_9.01. In another aspect, a method comprises concurrently detecting NLB resistance QTLs NLB_4.01 and NLB_4.02 and at least three or more NLB resistance QTLs selected from the group consisting of NLB_2.01, NLB_3.01, NLB_5.01, NLB_6.01, NLB_7.01, and NLB_9.01. In another aspect, a method comprises concurrently detecting NLB resistance QTLs NLB_4.01 and NLB_4.02 and at least four or more NLB resistance QTLs selected from the group consisting of NLB_2.01, NLB_3.01, NLB_5.01, NLB_6.01, NLB_7.01, and NLB_9.01. In another aspect, a method comprises concurrently detecting NLB resistance QTLs NLB_4.01 and NLB_4.02 and at least five or more NLB resistance QTLs selected from the group consisting of NLB_2.01, NLB_3.01, NLB_5.01, NLB_6.01, NLB_7.01, and NLB_9.01.
[0083] In another aspect, a method comprises concurrently detecting NLB resistance QTLs NLB_3.01, NLB_4.01, and NLB_4.02. In another aspect, a method comprises concurrently detecting NLB resistance QTLs NLB_3.01, NLB_4.01, and NLB_4.02 and at least one or more NLB resistance QTLs selected from the group consisting of NLB_2.01, NLB_5.01, NLB_6.01, NLB_7.01, and NLB_9.01. In another aspect, a method comprises concurrently detecting NLB resistance QTLs NLB_3.01, NLB_4.01, and NLB_4.02 and at least two or more NLB resistance QTLs selected from the group consisting of NLB_2.01, NLB_5.01, NLB_6.01, NLB_7.01, and NLB_9.01. In another aspect, a method comprises concurrently detecting NLB resistance QTLs NLB_3.01, NLB_4.01, and NLB_4.02 and at least three or more NLB resistance QTLs selected from the group consisting of NLB_2.01, NLB_5.01, NLB_6.01, NLB_7.01, and NLB_9.01. In another aspect, a method comprises concurrently detecting NLB resistance QTLs NLB_3.01, NLB_4.01, and NLB_4.02 and at least four or more NLB resistance QTLs selected from the group consisting of NLB_2.01, NLB_5.01, NLB_6.01, NLB_7.01, and NLB_9.01.
[0084] In another aspect, a method comprises concurrently detecting NLB resistance QTLs NLB_3.01 and NLB_4.01. In another aspect, a method comprises concurrently detecting NLB resistance QTLs NLB_3.01 and NLB_4.01 and at least one or more NLB resistance QTLs selected from the group consisting of NLB_2.01, NLB_4.02, NLB_5.01, NLB_6.01, NLB_7.01, and NLB_9.01. In another aspect, a method comprises concurrently detecting NLB resistance QTLs NLB_3.01 and NLB_4.01 and at least two or more NLB resistance QTLs selected from the group consisting of NLB_2.01, NLB_4.02, NLB_5.01, NLB_6.01, NLB_7.01, and NLB_9.01. In another aspect, a method comprises concurrently detecting NLB resistance QTLs NLB_3.01 and NLB_4.01 and at least three or more NLB resistance QTLs selected from the group consisting of NLB_2.01, NLB_4.02, NLB_5.01, NLB_6.01, NLB_7.01, and NLB_9.01. In another aspect, a method comprises concurrently detecting NLB resistance QTLs NLB_3.01 and NLB_4.01 and at least four or more NLB resistance QTLs selected from the group consisting of NLB_2.01, NLB_4.02, NLB_5.01, NLB_6.01, NLB_7.01, and NLB_9.01. In another aspect, a method comprises concurrently detecting NLB resistance QTLs NLB_3.01 and NLB_4.01 and at least five or more NLB resistance QTLs selected from the group consisting of NLB_2.01, NLB_4.02, NLB_5.01, NLB_6.01, NLB_7.01, and NLB_9.01.
[0085] In another aspect, a method comprises concurrently detecting NLB resistance QTLs NLB_3.01 and NLB_4.02. In another aspect, a method comprises concurrently detecting NLB resistance QTLs NLB_3.01 and NLB_4.02 and at least one or more NLB resistance QTLs selected from the group consisting of NLB_2.01, NLB_4.01, NLB_5.01, NLB_6.01, NLB_7.01, and NLB_9.01. In another aspect, a method comprises concurrently detecting NLB resistance QTLs NLB_3.01 and NLB_4.02 and at least two or more NLB resistance QTLs selected from the group consisting of NLB_2.01, NLB_4.01, NLB_5.01, NLB_6.01, NLB_7.01, and NLB_9.01. In another aspect, a method comprises concurrently detecting NLB resistance QTLs NLB_3.01 and NLB_4.02 and at least three or more NLB resistance QTLs selected from the group consisting of NLB_2.01, NLB_4.01, NLB_5.01, NLB_6.01, NLB_7.01, and NLB_9.01. In another aspect, a method comprises concurrently detecting NLB resistance QTLs NLB_3.01 and NLB_4.02 and at least four or more NLB resistance QTLs selected from the group consisting of NLB_2.01, NLB_4.01, NLB_5.01, NLB_6.01, NLB_7.01, and NLB_9.01. In another aspect, a method comprises concurrently detecting NLB resistance QTLs NLB_3.01 and NLB_4.02 and at least five or more NLB resistance QTLs selected from the group consisting of NLB_2.01, NLB_4.01, NLB_5.01, NLB_6.01, NLB_7.01, and NLB_9.01.
[0086] In an aspect, a method comprises concurrently detecting NLB resistance QTLs NLB_2.01, NLB_4.01, and NLB_4.02. In another aspect, a method comprises concurrently detecting NLB resistance QTLs NLB_2.01, NLB_4.01, and NLB_4.02 and at least one or more NLB resistance QTLs selected from the group consisting of NLB_3.01, NLB_5.01, NLB_6.01, NLB_7.01, and NLB_9.01. In another aspect, a method comprises concurrently detecting NLB resistance QTLs NLB_2.01, NLB_4.01, and NLB_4.02 and at least two or more NLB resistance QTLs selected from the group consisting of NLB_3.01, NLB_5.01, NLB_6.01, NLB_7.01, and NLB_9.01. In another aspect, a method comprises concurrently detecting NLB resistance QTLs NLB_2.01, NLB_4.01, and NLB_4.02 and at least three or more NLB resistance QTLs selected from the group consisting of NLB_3.01, NLB_5.01, NLB_6.01, NLB_7.01, and NLB_9.01. In another aspect, a method comprises concurrently detecting NLB resistance QTLs NLB_2.01, NLB_4.01, and NLB_4.02 and at least four or more NLB resistance QTLs selected from the group consisting of NLB_3.01, NLB_5.01, NLB_6.01, NLB_7.01, and NLB_9.01.
[0087] In an aspect, a method comprises concurrently detecting NLB resistance QTLs NLB_2.01 and NLB_4.01. In another aspect, a method comprises concurrently detecting NLB resistance QTLs NLB_2.01 and NLB_4.01 and at least one or more NLB resistance QTLs selected from the group consisting of NLB_3.01, NLB_4.02, NLB_5.01, NLB_6.01, NLB_7.01, and NLB_9.01. In another aspect, a method comprises concurrently detecting NLB resistance QTLs NLB_2.01 and NLB_4.01 and at least two or more NLB resistance QTLs selected from the group consisting of NLB_3.01, NLB_4.02, NLB_5.01, NLB_6.01, NLB_7.01, and NLB_9.01. In another aspect, a method comprises concurrently detecting NLB resistance QTLs NLB_2.01 and NLB_4.01 and at least three or more NLB resistance QTLs selected from the group consisting of NLB_3.01, NLB_4.02, NLB_5.01, NLB_6.01, NLB_7.01, and NLB_9.01. In another aspect, a method comprises concurrently detecting NLB resistance QTLs NLB_2.01 and NLB_4.01 and at least four or more NLB resistance QTLs selected from the group consisting of NLB_3.01, NLB_4.02, NLB_5.01, NLB_6.01, NLB_7.01, and NLB_9.01. In another aspect, a method comprises concurrently detecting NLB resistance QTLs NLB_2.01 and NLB_4.01 and at least five or more NLB resistance QTLs selected from the group consisting of NLB_3.01, NLB_4.02, NLB_5.01, NLB_6.01, NLB_7.01, and NLB_9.01.
[0088] In an aspect, a method comprises concurrently detecting NLB resistance QTLs NLB_2.01 and NLB_4.02. In another aspect, a method comprises concurrently detecting NLB resistance QTLs NLB_2.01 and NLB_4.02 and at least one or more NLB resistance QTLs selected from the group consisting of NLB_3.01, NLB_4.01, NLB_5.01, NLB_6.01, NLB_7.01, and NLB_9.01. In another aspect, a method comprises concurrently detecting NLB resistance QTLs NLB_2.01 and NLB_4.02 and at least two or more NLB resistance QTLs selected from the group consisting of NLB_3.01, NLB_4.01, NLB_5.01, NLB_6.01, NLB_7.01, and NLB_9.01. In another aspect, a method comprises concurrently detecting NLB resistance QTLs NLB_2.01 and NLB_4.02 and at least three or more NLB resistance QTLs selected from the group consisting of NLB_3.01, NLB_4.01, NLB_5.01, NLB_6.01, NLB_7.01, and NLB_9.01. In another aspect, a method comprises concurrently detecting NLB resistance QTLs NLB_2.01 and NLB_4.02 and at least four or more NLB resistance QTLs selected from the group consisting of NLB_3.01, NLB_4.01, NLB_5.01, NLB_6.01, NLB_7.01, and NLB_9.01. In another aspect, a method comprises concurrently detecting NLB resistance QTLs NLB_2.01 and NLB_4.02 and at least five or more NLB resistance QTLs selected from the group consisting of NLB_3.01, NLB_4.01, NLB_5.01, NLB_6.01, NLB_7.01, and NLB_9.01.
[0089] In another aspect, a method comprises concurrently detecting NLB resistance QTL NLB_4.01 and at least one or more NLB resistance loci selected from the group consisting of Ht1, Ht2, Ht3, and Htn1. In another aspect, a method comprises concurrently detecting NLB resistance QTL NLB_4.01 and at least two or more NLB resistance loci selected from the group consisting of Ht1, Ht2, Ht3, and Htn1. In another aspect, a method comprises concurrently detecting NLB resistance QTL NLB_4.01 and at least three or more NLB resistance loci selected from the group consisting of Ht1, Ht2, Ht3, and Htn1. In another aspect, a method comprises concurrently detecting NLB resistance QTL NLB_4.02 and at least one or more NLB resistance loci selected from the group consisting of Ht1, Ht2, Ht3, and Htn1. In another aspect, a method comprises concurrently detecting NLB resistance QTL NLB_4.02 and at least two or more NLB resistance loci selected from the group consisting of Ht1, Ht2, Ht3, and Htn1. In another aspect, a method comprises concurrently detecting NLB resistance QTL NLB_4.02 and at least three or more NLB resistance loci selected from the group consisting of Ht1, Ht2, Ht3, and Htn1. In another aspect, a method comprises concurrently detecting NLB resistance QTLs NLB_4.01 and NLB_4.02 and at least one or more NLB resistance loci selected from the group consisting of Ht1, Ht2, Ht3, and Htn1. In another aspect, a method comprises concurrently detecting NLB resistance QTLs NLB_4.01 and NLB_4.02 and at least two or more NLB resistance loci selected from the group consisting of Ht1, Ht2, Ht3, and Htn1. In another aspect, a method comprises concurrently detecting NLB resistance QTLs NLB_4.01 and NLB_4.02 and at least three or more NLB resistance loci selected from the group consisting of Ht1, Ht2, Ht3, and Htn1.
[0090] In another aspect, a method comprises concurrently detecting NLB resistance QTL NLB_2.01 and one or more NLB resistance QTLs selected from the group consisting of NLB resistance QTLs NLB_3.01, NLB_4.01, NLB_4.02, NLB_5.01, NLB_6.01, NLB_7.01, and NLB_9.01. In another aspect, a method comprises concurrently detecting NLB resistance QTL NLB_2.01 and two or more NLB resistance QTLs selected from the group consisting of NLB resistance QTLs NLB_3.01, NLB_4.01, NLB_4.02, NLB_5.01, NLB_6.01, NLB_7.01, and NLB_9.01. In another aspect, a method comprises concurrently detecting NLB resistance QTL NLB_2.01 and three or more NLB resistance QTLs selected from the group consisting of NLB resistance QTLs NLB_3.01, NLB_4.01, NLB_4.02, NLB_5.01, NLB_6.01, NLB_7.01, and NLB_9.01. In another aspect, a method comprises concurrently detecting NLB resistance QTL NLB_2.01 and four or more NLB resistance QTLs selected from the group consisting of NLB resistance QTLs NLB_3.01, NLB_4.01, NLB_4.02, NLB_5.01, NLB_6.01, NLB_7.01, and NLB_9.01. In another aspect, a method comprises concurrently detecting NLB resistance QTL NLB_2.01 and five or more NLB resistance QTLs selected from the group consisting of NLB resistance QTLs NLB_3.01, NLB_4.01, NLB_4.02, NLB_5.01, NLB_6.01, NLB_7.01, and NLB_9.01. In another aspect, a method comprises concurrently detecting NLB resistance QTL NLB_2.01 and or six or more NLB resistance QTLs selected from the group consisting of NLB resistance QTLs NLB_3.01, NLB_4.01, NLB_4.02, NLB_5.01, NLB_6.01, NLB_7.01, and NLB_9.01. In another aspect, a method comprises concurrently detecting NLB resistance QTL NLB_2.01 and NLB resistance QTLs NLB_3.01, NLB_4.01, NLB_4.02, NLB_5.01, NLB_6.01, NLB_7.01, and NLB_9.01.
[0091] In another aspect, a method comprises concurrently detecting NLB resistance QTL NLB_3.01 and one or more NLB resistance QTLs selected from the group consisting of NLB resistance QTLs NLB_2.01, NLB_4.01, NLB_4.02, NLB_5.01, NLB_6.01, NLB_7.01, and NLB_9.01. In another aspect, a method comprises concurrently detecting NLB resistance QTL NLB_3.01 and two or more NLB resistance QTLs selected from the group consisting of NLB resistance QTLs NLB_2.01, NLB_4.01, NLB_4.02, NLB_5.01, NLB_6.01, NLB_7.01, and NLB_9.01. In another aspect, a method comprises concurrently detecting NLB resistance QTL NLB_3.01 and three or more NLB resistance QTLs selected from the group consisting of NLB resistance QTLs NLB_2.01, NLB_4.01, NLB_4.02, NLB_5.01, NLB_6.01, NLB_7.01, and NLB_9.01. In another aspect, a method comprises concurrently detecting NLB resistance QTL NLB_3.01 and four or more NLB resistance QTLs selected from the group consisting of NLB resistance QTLs NLB_2.01, NLB_4.01, NLB_4.02, NLB_5.01, NLB_6.01, NLB_7.01, and NLB_9.01. In another aspect, a method comprises concurrently detecting NLB resistance QTL NLB_3.01 and five or more NLB resistance QTLs selected from the group consisting of NLB resistance QTLs NLB_2.01, NLB_4.01, NLB_4.02, NLB_5.01, NLB_6.01, NLB_7.01, and NLB_9.01. In another aspect, a method comprises concurrently detecting NLB resistance QTL NLB_3.01 and or six or more NLB resistance QTLs selected from the group consisting of NLB resistance QTLs NLB_2.01, NLB_4.01, NLB_4.02, NLB_5.01, NLB_6.01, NLB_7.01, and NLB_9.01. In another aspect, a method comprises concurrently detecting NLB resistance QTL NLB_3.01 and NLB resistance QTLs NLB_2.01, NLB_4.01, NLB_4.02, NLB_5.01, NLB_6.01, NLB_7.01, and NLB_9.01.
[0092] In another aspect, a method comprises concurrently detecting NLB resistance QTL NLB_4.01 and one or more NLB resistance QTLs selected from the group consisting of NLB resistance QTLs NLB_2.01, NLB_3.01, NLB_4.02, NLB_5.01, NLB_6.01, NLB_7.01, and NLB_9.01. In another aspect, a method comprises concurrently detecting NLB resistance QTL NLB_4.01 and two or more NLB resistance QTLs selected from the group consisting of NLB resistance QTLs NLB_2.01, NLB_3.01, NLB_4.02, NLB_5.01, NLB_6.01, NLB_7.01, and NLB_9.01. In another aspect, a method comprises concurrently detecting NLB resistance QTL NLB_4.01 and three or more NLB resistance QTLs selected from the group consisting of NLB resistance QTLs NLB_2.01, NLB_3.01, NLB_4.02, NLB_5.01, NLB_6.01, NLB_7.01, and NLB_9.01. In another aspect, a method comprises concurrently detecting NLB resistance QTL NLB_4.01 and four or more NLB resistance QTLs selected from the group consisting of NLB resistance QTLs NLB_2.01, NLB_3.01, NLB_4.02, NLB_5.01, NLB_6.01, NLB_7.01, and NLB_9.01. In another aspect, a method comprises concurrently detecting NLB resistance QTL NLB_4.01 and five or more NLB resistance QTLs selected from the group consisting of NLB resistance QTLs NLB_2.01, NLB_3.01, NLB_4.02, NLB_5.01, NLB_6.01, NLB_7.01, and NLB_9.01. In another aspect, a method comprises concurrently detecting NLB resistance QTL NLB_4.01 and or six or more NLB resistance QTLs selected from the group consisting of NLB resistance QTLs NLB_2.01, NLB_3.01, NLB_4.02, NLB_5.01, NLB_6.01, NLB_7.01, and NLB_9.01. In another aspect, a method comprises concurrently detecting NLB resistance QTL NLB_4.01 and NLB resistance QTLs NLB_2.01, NLB_3.01, NLB_4.02, NLB_5.01, NLB_6.01, NLB_7.01, and NLB_9.01.
[0093] In another aspect, a method comprises concurrently detecting NLB resistance QTL NLB_4.02 and one or more NLB resistance QTLs selected from the group consisting of NLB resistance QTLs NLB_2.01, NLB_3.01, NLB_4.01, NLB_5.01, NLB_6.01, NLB_7.01, and NLB_9.01. In another aspect, a method comprises concurrently detecting NLB resistance QTL NLB_4.02 and two or more NLB resistance QTLs selected from the group consisting of NLB resistance QTLs NLB_2.01, NLB_3.01, NLB_4.01, NLB_5.01, NLB_6.01, NLB_7.01, and NLB_9.01. In another aspect, a method comprises concurrently detecting NLB resistance QTL NLB_4.02 and three or more NLB resistance QTLs selected from the group consisting of NLB resistance QTLs NLB_2.01, NLB_3.01, NLB_4.01, NLB_5.01, NLB_6.01, NLB_7.01, and NLB_9.01. In another aspect, a method comprises concurrently detecting NLB resistance QTL NLB_4.02 and four or more NLB resistance QTLs selected from the group consisting of NLB resistance QTLs NLB_2.01, NLB_3.01, NLB_4.01, NLB_5.01, NLB_6.01, NLB_7.01, and NLB_9.01. In another aspect, a method comprises concurrently detecting NLB resistance QTL NLB_4.02 and five or more NLB resistance QTLs selected from the group consisting of NLB resistance QTLs NLB_2.01, NLB_3.01, NLB_4.01, NLB_5.01, NLB_6.01, NLB_7.01, and NLB_9.01. In another aspect, a method comprises concurrently detecting NLB resistance QTL NLB_4.02 and or six or more NLB resistance QTLs selected from the group consisting of NLB resistance QTLs NLB_2.01, NLB_3.01, NLB_4.01, NLB_5.01, NLB_6.01, NLB_7.01, and NLB_9.01. In another aspect, a method comprises concurrently detecting NLB resistance QTL NLB_4.02 and NLB resistance QTLs NLB_2.01, NLB_3.01, NLB_4.01, NLB_5.01, NLB_6.01, NLB_7.01, and NLB_9.01.
[0094] In another aspect, a method comprises concurrently detecting NLB resistance QTL NLB_5.01 and one or more NLB resistance QTLs selected from the group consisting of NLB resistance QTLs NLB_2.01, NLB_3.01, NLB_4.01, NLB_4.02, NLB_6.01, NLB_7.01, and NLB_9.01. In another aspect, a method comprises concurrently detecting NLB resistance QTL NLB_5.01 and two or more NLB resistance QTLs selected from the group consisting of NLB resistance QTLs NLB_2.01, NLB_3.01, NLB_4.01, NLB_4.02, NLB_6.01, NLB_7.01, and NLB_9.01. In another aspect, a method comprises concurrently detecting NLB resistance QTL NLB_5.01 and three or more NLB resistance QTLs selected from the group consisting of NLB resistance QTLs NLB_2.01, NLB_3.01, NLB_4.01, NLB_4.02, NLB_6.01, NLB_7.01, and NLB_9.01. In another aspect, a method comprises concurrently detecting NLB resistance QTL NLB_5.01 and four or more NLB resistance QTLs selected from the group consisting of NLB resistance QTLs NLB_2.01, NLB_3.01, NLB_4.01, NLB_4.02, NLB_6.01, NLB_7.01, and NLB_9.01. In another aspect, a method comprises concurrently detecting NLB resistance QTL NLB_5.01 and five or more NLB resistance QTLs selected from the group consisting of NLB resistance QTLs NLB_2.01, NLB_3.01, NLB_4.01, NLB_4.02, NLB_6.01, NLB_7.01, and NLB_9.01. In another aspect, a method comprises concurrently detecting NLB resistance QTL NLB_5.01 and or six or more NLB resistance QTLs selected from the group consisting of NLB resistance QTLs NLB_2.01, NLB_3.01, NLB_4.01, NLB_4.02, NLB_6.01, NLB_7.01, and NLB_9.01. In another aspect, a method comprises concurrently detecting NLB resistance QTL NLB_5.01 and NLB resistance QTLs NLB_2.01, NLB_3.01, NLB_4.01, NLB_4.02, NLB_6.01, NLB_7.01, and NLB_9.01.
[0095] In another aspect, a method comprises concurrently detecting NLB resistance QTL NLB_6.01 and one or more NLB resistance QTLs selected from the group consisting of NLB resistance QTLs NLB_2.01, NLB_3.01, NLB_4.01, NLB_4.02, NLB_5.01, NLB_7.01, and NLB_9.01. In another aspect, a method comprises concurrently detecting NLB resistance QTL NLB_6.01 and two or more NLB resistance QTLs selected from the group consisting of NLB resistance QTLs NLB_2.01, NLB_3.01, NLB_4.01, NLB_4.02, NLB_5.01, NLB_7.01, and NLB_9.01. In another aspect, a method comprises concurrently detecting NLB resistance QTL NLB_6.01 and three or more NLB resistance QTLs selected from the group consisting of NLB resistance QTLs NLB_2.01, NLB_3.01, NLB_4.01, NLB_4.02, NLB_5.01, NLB_7.01, and NLB_9.01. In another aspect, a method comprises concurrently detecting NLB resistance QTL NLB_6.01 and four or more NLB resistance QTLs selected from the group consisting of NLB resistance QTLs NLB_2.01, NLB_3.01, NLB_4.01, NLB_4.02, NLB_5.01, NLB_7.01, and NLB_9.01. In another aspect, a method comprises concurrently detecting NLB resistance QTL NLB_6.01 and five or more NLB resistance QTLs selected from the group consisting of NLB resistance QTLs NLB_2.01, NLB_3.01, NLB_4.01, NLB_4.02, NLB_5.01, NLB_7.01, and NLB_9.01. In another aspect, a method comprises concurrently detecting NLB resistance QTL NLB_6.01 and or six or more NLB resistance QTLs selected from the group consisting of NLB resistance QTLs NLB_2.01, NLB_3.01, NLB_4.01, NLB_4.02, NLB_5.01, NLB_7.01, and NLB_9.01. In another aspect, a method comprises concurrently detecting NLB resistance QTL NLB_6.01 and NLB resistance QTLs NLB_2.01, NLB_3.01, NLB_4.01, NLB_4.02, NLB_5.01, NLB_7.01, and NLB_9.01.
[0096] In another aspect, a method comprises concurrently detecting NLB resistance QTL NLB_7.01 and one or more NLB resistance QTLs selected from the group consisting of NLB resistance QTLs NLB_2.01, NLB_3.01, NLB_4.01, NLB_4.02, NLB_5.01, NLB_6.01, and NLB_9.01. In another aspect, a method comprises concurrently detecting NLB resistance QTL NLB_7.01 and two or more NLB resistance QTLs selected from the group consisting of NLB resistance QTLs NLB_2.01, NLB_3.01, NLB_4.01, NLB_4.02, NLB_5.01, NLB_6.01, and NLB_9.01. In another aspect, a method comprises concurrently detecting NLB resistance QTL NLB_7.01 and three or more NLB resistance QTLs selected from the group consisting of NLB resistance QTLs NLB_2.01, NLB_3.01, NLB_4.01, NLB_4.02, NLB_5.01, NLB_6.01, and NLB_9.01. In another aspect, a method comprises concurrently detecting NLB resistance QTL NLB_7.01 and four or more NLB resistance QTLs selected from the group consisting of NLB resistance QTLs NLB_2.01, NLB_3.01, NLB_4.01, NLB_4.02, NLB_5.01, NLB_6.01, and NLB_9.01. In another aspect, a method comprises concurrently detecting NLB resistance QTL NLB_7.01 and five or more NLB resistance QTLs selected from the group consisting of NLB resistance QTLs NLB_2.01, NLB_3.01, NLB_4.01, NLB_4.02, NLB_5.01, NLB_6.01, and NLB_9.01. In another aspect, a method comprises concurrently detecting NLB resistance QTL NLB_7.01 and or six or more NLB resistance QTLs selected from the group consisting of NLB resistance QTLs NLB_2.01, NLB_3.01, NLB_4.01, NLB_4.02, NLB_5.01, NLB_6.01, and NLB_9.01. In another aspect, a method comprises concurrently detecting NLB resistance QTL NLB_7.01 and NLB resistance QTLs NLB_2.01, NLB_3.01, NLB_4.01, NLB_4.02, NLB_5.01, NLB_6.01, and NLB_9.01.
[0097] In another aspect, a method comprises concurrently detecting NLB resistance QTL NLB_9.01 and one or more NLB resistance QTLs selected from the group consisting of NLB resistance QTLs NLB_2.01, NLB_3.01, NLB_4.01, NLB_4.02, NLB_5.01, NLB_6.01, and NLB_7.01. In another aspect, a method comprises concurrently detecting NLB resistance QTL NLB_9.01 and two or more NLB resistance QTLs selected from the group consisting of NLB resistance QTLs NLB_2.01, NLB_3.01, NLB_4.01, NLB_4.02, NLB_5.01, NLB_6.01, and NLB_7.01. In another aspect, a method comprises concurrently detecting NLB resistance QTL NLB_9.01 and three or more NLB resistance QTLs selected from the group consisting of NLB resistance QTLs NLB_2.01, NLB_3.01, NLB_4.01, NLB_4.02, NLB_5.01, NLB_6.01, and NLB_7.01. In another aspect, a method comprises concurrently detecting NLB resistance QTL NLB_9.01 and four or more NLB resistance QTLs selected from the group consisting of NLB resistance QTLs NLB_2.01, NLB_3.01, NLB_4.01, NLB_4.02, NLB_5.01, NLB_6.01, and NLB_7.01. In another aspect, a method comprises concurrently detecting NLB resistance QTL NLB_9.01 and five or more NLB resistance QTLs selected from the group consisting of NLB resistance QTLs NLB_2.01, NLB_3.01, NLB_4.01, NLB_4.02, NLB_5.01, NLB_6.01, and NLB_7.01. In another aspect, a method comprises concurrently detecting NLB resistance QTL NLB_9.01 and or six or more NLB resistance QTLs selected from the group consisting of NLB resistance QTLs NLB_2.01, NLB_3.01, NLB_4.01, NLB_4.02, NLB_5.01, NLB_6.01, and NLB_7.01. In another aspect, a method comprises concurrently detecting NLB resistance QTL NLB_9.01 and NLB resistance QTLs NLB_2.01, NLB_3.01, NLB_4.01, NLB_4.02, NLB_5.01, NLB_6.01, and NLB_7.01.
[0098] In an aspect, this disclosure provides a method of producing a corn plant with enhanced NLB resistance, which method comprising the steps of: (a) crossing a first corn plant comprising an NLB resistance QTL with a second corn plant of a different genotype to produce one or more progeny plants or seeds; (b) selecting a progeny plant or seed comprising an NLB resistance allele of a polymorphic locus linked to an NLB resistance QTL, wherein a polymorphic locus is in a chromosomal segment flanked by any two of marker loci SEQ ID NOs: 1 to 18, any two of marker loci SEQ ID NOs: 19 to 31, any two of marker loci SEQ ID NOs: 32 to 52 and 471 to 475, any two of marker loci SEQ ID NOs: 53 to 65 and 446 to 468, any two of marker loci SEQ ID NOs: 66 to 84, any two of marker loci SEQ ID NOs: 85 to 89 and 476 to 482, or marker loci SEQ ID NOs: 469 and 470; (c) crossing the selected progeny plant with itself or the second corn plant to produce one or more further progeny plants or seeds; and (d) selecting a further progeny plant or seed comprising the NLB resistance allele. In an aspect, the further progeny plant in step (d) is an F.sub.2 to F.sub.7 progeny plant. In another aspect, the further progeny plant in step (d) comprises 2 to 7 generations of backcrossing. In yet another aspect, a method comprises using marker-assisted selection to select an NLB resistance allele in at least one polymorphic locus selected from the group consisting of SEQ ID NOs: 1-89 and 446-482.
[0099] In an aspect, this disclosure provides a method of obtaining a corn plant, seed, or cell with enhanced NLB resistance, which method comprises the steps of: (a) detecting in a population of corn plants, seeds, or cells a plant or seed comprising an NLB resistance allele at a polymorphic locus in a chromosomal segment flanked by SEQ ID NOs: 1 to 18, any two of marker loci SEQ ID NOs: 19 to 31, any two of marker loci SEQ ID NOs: 32 to 52 and 471 to 475, any two of marker loci SEQ ID NOs: 53 to 65 and 446 to 468, any two of marker loci SEQ ID NOs: 66 to 84, any two of marker loci SEQ ID NOs: 85 to 89 and 476 to 482, or marker loci SEQ ID NOs: 469 and 470; and (b) selecting the corn plant, seed, or cell from the population based on the presence of the NLB resistance allele.
[0100] In an aspect, this disclosure provides a method of producing a corn plant with enhanced NLB resistance, which method comprising the steps of: (a) crossing a first corn plant comprising an NLB resistance haplotype with a second corn plant of a different genotype to produce one or more progeny plants or seeds; (b) selecting a progeny plant or seed based on the presence of the NLB resistance haplotype, wherein the haplotype comprises resistance alleles of two or more polymorphic loci in a chromosomal interval flanked by: any two marker loci selected from the group consisting of SEQ ID NOs: 1 to 18, any two of marker loci SEQ ID NOs: 19 to 31, any two of marker loci SEQ ID NOs: 32 to 52 and 471 to 475, any two of marker loci SEQ ID NOs: 53 to 65 and 446 to 468, any two of marker loci SEQ ID NOs: 66 to 84, any two of marker loci SEQ ID NOs: 85 to 89 and 476 to 482, or marker loci SEQ ID NOs: 469 and 470.
[0101] In an aspect, this disclosure provides a method of obtaining a corn plant, seed, or cell with enhanced NLB resistance, which method comprising the steps of: (a) detecting in a population of corn plants, seeds, or cells a plant or seed comprising an NLB resistance haplotype, wherein the haplotype comprises resistance alleles of two or more polymorphic loci in a chromosomal interval flanked by: any two marker loci selected from the group consisting of SEQ ID NOs: 12 to 15; any two marker loci selected from the group consisting of SEQ ID NOs: 22 to 25; any two marker loci selected from the group consisting of SEQ ID NOs: 37 to 42 and 474; any two marker loci selected from the group consisting of SEQ ID NOs: 44 to 49; any two marker loci selected from the group consisting of SEQ ID NOs: 57 to 62 and 458 to 466; any two marker loci selected from the group consisting of SEQ ID NOs: 79 to 81; any two marker loci selected from the group consisting of SEQ ID NOs: 87 to 89 and 477 to 480; and marker loci SEQ ID NOs: 469 and 470; and (b) selecting a corn plant, seed, or cell from the population based on the presence of the NLB resistance haplotype. In another aspect, an NLB resistance haplotype comprises resistance alleles of two or more polymorphic loci selected from the group consisting of SEQ ID NOs: 12-15; 22-24; 37-41 and 474; 44-46; 60-62 and 464-466; 79-81; 87-89, 477, and 480; and 469-470.
[0102] In an aspect, this disclosure provides a method of obtaining a corn plant, seed, or cell with enhanced NLB resistance, which method comprising the steps of: (a) detecting in a population of corn plants, seeds, or cells a corn plant, seed, or cell comprising an NLB resistance haplotype, wherein the haplotype comprises resistance alleles of two or more polymorphic loci in a chromosomal interval flanked by: any two marker loci selected from the group consisting of SEQ ID NOs: 8 to 18; any two marker loci selected from the group consisting of SEQ ID NOs: 21 to 29; any two marker loci selected from the group consisting of SEQ ID NOs: 33 to 42, 473, and 474; any two marker loci selected from the group consisting of SEQ ID NOs: 43 to 49 and 475; any two marker loci selected from the group consisting of SEQ ID NOs: 57 to 64 and 458 to 468; any two marker loci selected from the group consisting of SEQ ID NOs: 74 to 82; any two marker loci selected from the group consisting of SEQ ID NOs: 86 to 89, 476, 477, 479, and 480; and marker loci SEQ ID NOs: 469 and 470; and (b) selecting a corn plant, seed, or cell from the population based on the presence of the NLB resistance haplotype. In yet another aspect, an NLB resistance haplotype comprises resistance alleles of two or more polymorphic loci selected from the group consisting of SEQ ID NOs: 8-18; 21-29; 33-42, 473, and 474; 43-49 and 475; 57-64 and 458-468; 74-82; 86-89, 476, 477, 479, and 480; and 469-470.
[0103] In an aspect, this disclosure provides a method for selecting a corn plant, seed, or cell, which method comprising the steps of: (a) isolating nucleic acids from a corn plant, seed, or cell; (b) analyzing the nucleic acids to detect a polymorphic marker associated with an NLB resistance QTL selected from the group consisting of NLB resistance QTLs NLB_2.01, NLB_3.01, NLB_4.01, NLB_4.02, NLB_5.01, NLB_6.01, NLB_7.01, and NLB_9.01; and (c) selecting a corn plant, seed, or cell comprising the NLB resistance QTL.
[0104] In an aspect, this disclosure provides a method for selecting a corn plant, seed, or cell, which method comprising the steps of: (a) detecting in a population of corn plants, seeds, or cells a corn plant, seed, or cell comprising an NLB resistance allele of a marker locus associated with an NLB resistance QTL selected from the group consisting of NLB resistance QTLs NLB_2.01, NLB_3.01, NLB_4.01, NLB_4.02, NLB_5.01, NLB_6.01, NLB_7.01, and NLB_9.01; and (b) selecting a corn plant, seed, or cell comprising the NLB resistance allele.
[0105] In an aspect, this disclosure provides a method for evaluating a collection of corn germplasm, which method comprising the steps of: (a) obtaining a collection of corn germplasm; (b) isolating nucleic acids from each germplasm; (c) assaying the nucleic acids for one or more markers linked to an NLB resistance QTL selected from the group consisting of NLB resistance QTLs NLB_2.01, NLB_3.01, NLB_4.01, NLB_4.02, NLB_5.01, NLB_6.01, NLB_7.01, and NLB_9.01; and (d) selecting germplasm comprising an NLB resistance QTL based on the marker assay.
[0106] In an aspect, a method provided herein comprises genotyping by a marker assay. As an example, a method provided herein comprises marker-assisted selection. As another example, a method provided herein comprises assaying a SNP marker. In yet another example, a method provided herein comprises the use of an oligonucleotide probe. In a further example, a method provided herein comprises using an oligonucleotide probe adjacent to a polymorphic nucleotide position in a marker locus being genotyped.
[0107] As an example, a corn plant or seed provided herein can be an inbred, a hybrid, a transgenic, a haploid, a doubled haploid, or in an agronomically elite background. These groups are not mutually exclusive, and a corn plant or seed could be in two or more groups (e.g., a plant could be a transgenic hybrid, another plant could be an inbred doubled haploid, etc.).
[0108] In an aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a polymorphic marker locus within about 20 cM of any one of marker loci SEQ ID NOs: 1-89 and 446-482. In an aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a polymorphic marker locus within about 15 cM of any one of marker loci SEQ ID NOs: 1-89 and 446-482. In an aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a polymorphic marker locus within about 10 cM of any one of marker loci SEQ ID NOs: 1-89 and 446-482. In an aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a polymorphic marker locus within about 5 cM of any one of marker loci SEQ ID NOs: 1-89 and 446-482. In an aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a polymorphic marker locus within about 4 cM of any one of marker loci SEQ ID NOs: 1-89 and 446-482. In an aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a polymorphic marker locus within about 3 cM of any one of marker loci SEQ ID NOs: 1-89 and 446-482. In an aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a polymorphic marker locus within about 2 cM of any one of marker loci SEQ ID NOs: 1-89 and 446-482. In an aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a polymorphic marker locus within about 1 cM of any one of marker loci SEQ ID NOs: 1-89 and 446-482. In an aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a polymorphic marker locus within about 0.5 cM of any one of marker loci SEQ ID NOs: 1-89 and 446-482. In an aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a polymorphic marker locus within less than about 0.5 cM of any one of marker loci SEQ ID NOs: 1-89 and 446-482. In an aspect, this disclosure provides a method comprising genotyping a polymorphic locus selected from the group consisting of SEQ ID NOs: 1-89 and 446-482.
[0109] In an aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a marker locus associated with NLB resistance QTL NLB_2.01, which NLB resistance QTL NLB_2.01 is located in a chromosomal interval flanked by any two of the marker loci selected from the group consisting of SEQ ID NOs: 1 to 18. In another aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a marker locus associated with NLB resistance QTL NLB_2.01, which NLB resistance QTL NLB_2.01 is located in a chromosomal interval flanked by any two of the marker loci selected from the group consisting of SEQ ID NOs: 12 to 15. In another aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a marker locus associated with NLB resistance QTL NLB_2.01, which NLB resistance QTL NLB_2.01 is located in a chromosomal interval flanked by any two of the marker loci selected from the group consisting of SEQ ID NOs: 8 to 18.
[0110] In an aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a marker locus within about 20 cM of NLB resistance QTL NLB_2.01, which NLB resistance QTL NLB_2.01 is located in a chromosomal interval flanked by any two of the marker loci selected from the group consisting of SEQ ID NOs: 1 to 18. In an aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a marker locus within about 15 cM of NLB resistance QTL NLB_2.01, which NLB resistance QTL NLB_2.01 is located in a chromosomal interval flanked by any two of the marker loci selected from the group consisting of SEQ ID NOs: 1 to 18. In an aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a marker locus within about 10 cM of NLB resistance QTL NLB_2.01, which NLB resistance QTL NLB_2.01 is located in a chromosomal interval flanked by any two of the marker loci selected from the group consisting of SEQ ID NOs: 1 to 18. In an aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a marker locus within about 5 cM of NLB resistance QTL NLB_2.01, which NLB resistance QTL NLB_2.01 is located in a chromosomal interval flanked by any two of the marker loci selected from the group consisting of SEQ ID NOs: 1 to 18. In an aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a marker locus within about 4 cM of NLB resistance QTL NLB_2.01, which NLB resistance QTL NLB_2.01 is located in a chromosomal interval flanked by any two of the marker loci selected from the group consisting of SEQ ID NOs: 1 to 18. In an aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a marker locus within about 3 cM of NLB resistance QTL NLB_2.01, which NLB resistance QTL NLB_2.01 is located in a chromosomal interval flanked by any two of the marker loci selected from the group consisting of SEQ ID NOs: 1 to 18. In an aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a marker locus within about 2 cM of NLB resistance QTL NLB_2.01, which NLB resistance QTL NLB_2.01 is located in a chromosomal interval flanked by any two of the marker loci selected from the group consisting of SEQ ID NOs: 1 to 18. In an aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a marker locus within about 1 cM of NLB resistance QTL NLB_2.01, which NLB resistance QTL NLB_2.01 is located in a chromosomal interval flanked by any two of the marker loci selected from the group consisting of SEQ ID NOs: 1 to 18. In an aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a marker locus within about 0.5 cM of NLB resistance QTL NLB_2.01, which NLB resistance QTL NLB_2.01 is located in a chromosomal interval flanked by any two of the marker loci selected from the group consisting of SEQ ID NOs: 1 to 18. In an aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a marker locus within less than about 0.5 cM of NLB resistance QTL NLB_2.01, which NLB resistance QTL NLB_2.01 is located in a chromosomal interval flanked by any two of the marker loci selected from the group consisting of SEQ ID NOs: 1 to 18. In an aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a marker locus associated with NLB resistance QTL NLB_3.01, which NLB resistance QTL NLB_3.01 is located in a chromosomal interval flanked by any two of the marker loci selected from the group consisting of SEQ ID NOs: 19 to 31. In another aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a marker locus associated with NLB resistance QTL NLB_3.01, which NLB resistance QTL NLB_3.01 is located in a chromosomal interval flanked by any two of the marker loci selected from the group consisting of SEQ ID NOs: 22 to 25. In another aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a marker locus associated with NLB resistance QTL NLB_3.01, which NLB resistance QTL NLB_3.01 is located in a chromosomal interval flanked by any two of the marker loci selected from the group consisting of SEQ ID NOs: 21 to 29.
[0111] In an aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a marker locus associated with and within about 20 cM of NLB resistance QTL NLB_3.01, which NLB resistance QTL NLB_3.01 is located in a chromosomal interval flanked by any two of the marker loci selected from the group consisting of SEQ ID NOs: 19 to 31. In an aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a marker locus associated with and within about 15 cM of NLB resistance QTL NLB_3.01, which NLB resistance QTL NLB_3.01 is located in a chromosomal interval flanked by any two of the marker loci selected from the group consisting of SEQ ID NOs: 19 to 31. In an aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a marker locus associated with and within about 10 cM of NLB resistance QTL NLB_3.01, which NLB resistance QTL NLB_3.01 is located in a chromosomal interval flanked by any two of the marker loci selected from the group consisting of SEQ ID NOs: 19 to 31. In an aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a marker locus associated with and within about 5 cM of NLB resistance QTL NLB_3.01, which NLB resistance QTL NLB_3.01 is located in a chromosomal interval flanked by any two of the marker loci selected from the group consisting of SEQ ID NOs: 19 to 31. In an aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a marker locus associated with and within about 4 cM of NLB resistance QTL NLB_3.01, which NLB resistance QTL NLB_3.01 is located in a chromosomal interval flanked by any two of the marker loci selected from the group consisting of SEQ ID NOs: 19 to 31. In an aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a marker locus associated with and within about 3 cM of NLB resistance QTL NLB_3.01, which NLB resistance QTL NLB_3.01 is located in a chromosomal interval flanked by any two of the marker loci selected from the group consisting of SEQ ID NOs: 19 to 31. In an aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a marker locus associated with and within about 2 cM of NLB resistance QTL NLB_3.01, which NLB resistance QTL NLB_3.01 is located in a chromosomal interval flanked by any two of the marker loci selected from the group consisting of SEQ ID NOs: 19 to 31. In an aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a marker locus associated with and within about 1 cM of NLB resistance QTL NLB_3.01, which NLB resistance QTL NLB_3.01 is located in a chromosomal interval flanked by any two of the marker loci selected from the group consisting of SEQ ID NOs: 19 to 31. In an aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a marker locus associated with and within about 0.5 cM of NLB resistance QTL NLB_3.01, which NLB resistance QTL NLB_3.01 is located in a chromosomal interval flanked by any two of the marker loci selected from the group consisting of SEQ ID NOs: 19 to 31. In an aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a marker locus within less than about 0.5 cM of NLB resistance QTL NLB_3.01, which NLB resistance QTL NLB_3.01 is located in a chromosomal interval flanked by any two of the marker loci selected from the group consisting of SEQ ID NOs: 19 to 31.
[0112] In an aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a marker locus associated with NLB resistance QTL NLB_4.01, which NLB resistance QTL NLB_4.01 is located in a chromosomal interval flanked by any two of the marker loci selected from the group consisting of SEQ ID NOs: 32 to 52 and 471 to 475. In another aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a marker locus associated with NLB resistance QTL NLB_4.01, which NLB resistance QTL NLB_4.01 is located in a chromosomal interval flanked by any two of the marker loci selected from the group consisting of SEQ ID NOs: 37 to 42 and 474. In another aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a marker locus associated with NLB resistance QTL NLB_4.01, which NLB resistance QTL NLB_4.01 is located in a chromosomal interval flanked by any two of the marker loci selected from the group consisting of SEQ ID NOs: 33 to 42, 473, and 474.
[0113] In an aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a marker locus associated with and within about 20 cM of NLB resistance QTL NLB_4.01, which NLB resistance QTL NLB_4.01 is located in a chromosomal interval flanked by any two of the marker loci selected from the group consisting of SEQ ID NOs: 32 to 52 and 471 to 475. In an aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a marker locus associated with and within about 15 cM of NLB resistance QTL NLB_4.01, which NLB resistance QTL NLB_4.01 is located in a chromosomal interval flanked by any two of the marker loci selected from the group consisting of SEQ ID NOs: 32 to 52 and 471 to 475. In an aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a marker locus associated with and within about 10 cM of NLB resistance QTL NLB_4.01, which NLB resistance QTL NLB_4.01 is located in a chromosomal interval flanked by any two of the marker loci selected from the group consisting of SEQ ID NOs: 32 to 52 and 471 to 475. In an aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a marker locus associated with and within about 5 cM of NLB resistance QTL NLB_4.01, which NLB resistance QTL NLB_4.01 is located in a chromosomal interval flanked by any two of the marker loci selected from the group consisting of SEQ ID NOs: 32 to 52 and 471 to 475. In an aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a marker locus associated with and within about 4 cM of NLB resistance QTL NLB_4.01, which NLB resistance QTL NLB_4.01 is located in a chromosomal interval flanked by any two of the marker loci selected from the group consisting of SEQ ID NOs: 32 to 52 and 471 to 475. In an aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a marker locus associated with and within about 3 cM of NLB resistance QTL NLB_4.01, which NLB resistance QTL NLB_4.01 is located in a chromosomal interval flanked by any two of the marker loci selected from the group consisting of SEQ ID NOs: 32 to 52 and 471 to 475. In an aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a marker locus associated with and within about 2 cM of NLB resistance QTL NLB_4.01, which NLB resistance QTL NLB_4.01 is located in a chromosomal interval flanked by any two of the marker loci selected from the group consisting of SEQ ID NOs: 32 to 52 and 471 to 475. In an aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a marker locus associated with and within about 1 cM of NLB resistance QTL NLB_4.01, which NLB resistance QTL NLB_4.01 is located in a chromosomal interval flanked by any two of the marker loci selected from the group consisting of SEQ ID NOs: 32 to 52 and 471 to 475. In an aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a marker locus associated with and within about 0.5 cM of NLB resistance QTL NLB_4.01, which NLB resistance QTL NLB_4.01 is located in a chromosomal interval flanked by any two of the marker loci selected from the group consisting of SEQ ID NOs: 32 to 52 and 471 to 475. In an aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a marker locus within less than about 0.5 cM of NLB resistance QTL NLB_4.01, which NLB resistance QTL NLB_4.01 is located in a chromosomal interval flanked by any two of the marker loci selected from the group consisting of SEQ ID NOs: 32 to 52 and 471 to 475.
[0114] In an aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a marker locus associated with NLB resistance QTL NLB_4.02, which NLB resistance QTL NLB_4.02 is located in a chromosomal interval flanked by any two of the marker loci selected from the group consisting of SEQ ID NOs: 32 to 52 and 471 to 475. In another aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a marker locus associated with NLB resistance QTL NLB_4.02, which NLB resistance QTL NLB_4.02 is located in a chromosomal interval flanked by any two of the marker loci selected from the group consisting of SEQ ID NOs: 44 to 49. In another aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a marker locus associated with NLB resistance QTL NLB_4.02, which NLB resistance QTL NLB_4.02 is located in a chromosomal interval flanked by any two of the marker loci selected from the group consisting of SEQ ID NOs: 43 to 49 and 475.
[0115] In an aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a marker locus associated with and within about 20 cM of NLB resistance QTL NLB_4.02, which NLB resistance QTL NLB_4.02 is located in a chromosomal interval flanked by any two of the marker loci selected from the group consisting of SEQ ID NOs: 32 to 52 and 471 to 475. In an aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a marker locus associated with and within about 15 cM of NLB resistance QTL NLB_4.02, which NLB resistance QTL NLB_4.02 is located in a chromosomal interval flanked by any two of the marker loci selected from the group consisting of SEQ ID NOs: 32 to 52 and 471 to 475. In an aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a marker locus associated with and within about 10 cM of NLB resistance QTL NLB_4.02, which NLB resistance QTL NLB_4.02 is located in a chromosomal interval flanked by any two of the marker loci selected from the group consisting of SEQ ID NOs: 32 to 52 and 471 to 475. In an aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a marker locus associated with and within about 5 cM of NLB resistance QTL NLB_4.02, which NLB resistance QTL NLB_4.02 is located in a chromosomal interval flanked by any two of the marker loci selected from the group consisting of SEQ ID NOs: 32 to 52 and 471 to 475. In an aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a marker locus associated with and within about 4 cM of NLB resistance QTL NLB_4.02, which NLB resistance QTL NLB_4.02 is located in a chromosomal interval flanked by any two of the marker loci selected from the group consisting of SEQ ID NOs: 32 to 52 and 471 to 475. In an aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a marker locus associated with and within about 3 cM of NLB resistance QTL NLB_4.02, which NLB resistance QTL NLB_4.02 is located in a chromosomal interval flanked by any two of the marker loci selected from the group consisting of SEQ ID NOs: 32 to 52 and 471 to 475. In an aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a marker locus associated with and within about 2 cM of NLB resistance QTL NLB_4.02, which NLB resistance QTL NLB_4.02 is located in a chromosomal interval flanked by any two of the marker loci selected from the group consisting of SEQ ID NOs: 32 to 52 and 471 to 475. In an aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a marker locus associated with and within about 1 cM of NLB resistance QTL NLB_4.02, which NLB resistance QTL NLB_4.02 is located in a chromosomal interval flanked by any two of the marker loci selected from the group consisting of SEQ ID NOs: 32 to 52 and 471 to 475. In an aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a marker locus associated with and within about 0.5 cM of NLB resistance QTL NLB_4.02, which NLB resistance QTL NLB_4.02 is located in a chromosomal interval flanked by any two of the marker loci selected from the group consisting of SEQ ID NOs: 32 to 52 and 471 to 475. In an aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a marker locus within less than about 0.5 cM of NLB resistance QTL NLB_4.02, which NLB resistance QTL NLB_4.02 is located in a chromosomal interval flanked by any two of the marker loci selected from the group consisting of SEQ ID NOs: 32 to 52 and 471 to 475.
[0116] In an aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a marker locus associated with NLB resistance QTL NLB_5.01, which NLB resistance QTL NLB_5.01 is located in a chromosomal interval flanked by any two of the marker loci selected from the group consisting of SEQ ID NOs: 53 to 65 and 446 to 468. In another aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a marker locus associated with NLB resistance QTL NLB_5.01, which NLB resistance QTL NLB_5.01 is located in a chromosomal interval flanked by any two of the marker loci selected from the group consisting of SEQ ID NOs: 57 to 62 and 458 to 466. In another aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a marker locus associated with NLB resistance QTL NLB_5.01, which NLB resistance QTL NLB_5.01 is located in a chromosomal interval flanked by any two of the marker loci selected from the group consisting of SEQ ID NOs: 57 to 64 and 458 to 468.
[0117] In an aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a marker locus associated with and within about 20 cM of NLB resistance QTL NLB_5.01, which NLB resistance QTL NLB_5.01 is located in a chromosomal interval flanked by any two of the marker loci selected from the group consisting of SEQ ID NOs: 53 to 65 and 446 to 468. In an aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a marker locus associated with and within about 15 cM of NLB resistance QTL NLB_5.01, which NLB resistance QTL NLB_5.01 is located in a chromosomal interval flanked by any two of the marker loci selected from the group consisting of SEQ ID NOs: 53 to 65 and 446 to 468. In an aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a marker locus associated with and within about 10 cM of NLB resistance QTL NLB_5.01, which NLB resistance QTL NLB_5.01 is located in a chromosomal interval flanked by any two of the marker loci selected from the group consisting of SEQ ID NOs: 53 to 65 and 446 to 468. In an aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a marker locus associated with and within about 5 cM of NLB resistance QTL NLB_5.01, which NLB resistance QTL NLB_5.01 is located in a chromosomal interval flanked by any two of the marker loci selected from the group consisting of SEQ ID NOs: 53 to 65 and 446 to 468. In an aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a marker locus associated with and within about 4 cM of NLB resistance QTL NLB_5.01, which NLB resistance QTL NLB_5.01 is located in a chromosomal interval flanked by any two of the marker loci selected from the group consisting of SEQ ID NOs: 53 to 65 and 446 to 468. In an aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a marker locus associated with and within about 3 cM of NLB resistance QTL NLB_5.01, which NLB resistance QTL NLB_5.01 is located in a chromosomal interval flanked by any two of the marker loci selected from the group consisting of SEQ ID NOs: 53 to 65 and 446 to 468. In an aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a marker locus associated with and within about 2 cM of NLB resistance QTL NLB_5.01, which NLB resistance QTL NLB_5.01 is located in a chromosomal interval flanked by any two of the marker loci selected from the group consisting of SEQ ID NOs: 53 to 65 and 446 to 468. In an aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a marker locus associated with and within about 1 cM of NLB resistance QTL NLB_5.01, which NLB resistance QTL NLB_5.01 is located in a chromosomal interval flanked by any two of the marker loci selected from the group consisting of SEQ ID NOs: 53 to 65 and 446 to 468. In an aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a marker locus associated with and within about 0.5 cM of NLB resistance QTL NLB_5.01, which NLB resistance QTL NLB_5.01 is located in a chromosomal interval flanked by any two of the marker loci selected from the group consisting of SEQ ID NOs: 53 to 65 and 446 to 468. In an aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a marker locus within less than about 0.5 cM of NLB resistance QTL NLB_5.01, which NLB resistance QTL NLB_3.01 is located in a chromosomal interval flanked by any two of the marker loci selected from the group consisting of SEQ ID NOs: 53 to 65 and 446 to 468.
[0118] In an aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a marker locus associated with NLB resistance QTL NLB_6.01, which NLB resistance QTL NLB_6.01 is located in a chromosomal interval flanked by any two of the marker loci selected from the group consisting of SEQ ID NOs: 66 to 84. In another aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a marker locus associated with NLB resistance QTL NLB_6.01, which NLB resistance QTL NLB_6.01 is located in a chromosomal interval flanked by any two of the marker loci selected from the group consisting of SEQ ID NOs: 79 to 81. In another aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a marker locus associated with NLB resistance QTL NLB_6.01, which NLB resistance QTL NLB_6.01 is located in a chromosomal interval flanked by any two of the marker loci selected from the group consisting of SEQ ID NOs: 74 to 82.
[0119] In an aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a marker locus associated with and within about 20 cM of NLB resistance QTL NLB_6.01, which NLB resistance QTL NLB_6.01 is located in a chromosomal interval flanked by any two of the marker loci selected from the group consisting of SEQ ID NOs: 74 to 82. In an aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a marker locus associated with and within about 15 cM of NLB resistance QTL NLB_6.01, which NLB resistance QTL NLB_6.01 is located in a chromosomal interval flanked by any two of the marker loci selected from the group consisting of SEQ ID NOs: 74 to 82. In an aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a marker locus associated with and within about 10 cM of NLB resistance QTL NLB_6.01, which NLB resistance QTL NLB_6.01 is located in a chromosomal interval flanked by any two of the marker loci selected from the group consisting of SEQ ID NOs: 74 to 82. In an aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a marker locus associated with and within about 5 cM of NLB resistance QTL NLB_6.01, which NLB resistance QTL NLB_6.01 is located in a chromosomal interval flanked by any two of the marker loci selected from the group consisting of SEQ ID NOs: 74 to 82. In an aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a marker locus associated with and within about 4 cM of NLB resistance QTL NLB_6.01, which NLB resistance QTL NLB_6.01 is located in a chromosomal interval flanked by any two of the marker loci selected from the group consisting of SEQ ID NOs: 74 to 82. In an aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a marker locus associated with and within about 3 cM of NLB resistance QTL NLB_6.01, which NLB resistance QTL NLB_6.01 is located in a chromosomal interval flanked by any two of the marker loci selected from the group consisting of SEQ ID NOs: 74 to 82. In an aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a marker locus associated with and within about 2 cM of NLB resistance QTL NLB_6.01, which NLB resistance QTL NLB_6.01 is located in a chromosomal interval flanked by any two of the marker loci selected from the group consisting of SEQ ID NOs: 74 to 82. In an aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a marker locus associated with and within about 1 cM of NLB resistance QTL NLB_6.01, which NLB resistance QTL NLB_6.01 is located in a chromosomal interval flanked by any two of the marker loci selected from the group consisting of SEQ ID NOs: 74 to 82. In an aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a marker locus associated with and within about 0.5 cM of NLB resistance QTL NLB_6.01, which NLB resistance QTL NLB_6.01 is located in a chromosomal interval flanked by any two of the marker loci selected from the group consisting of SEQ ID NOs: 74 to 82. In an aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a marker locus within less than about 0.5 cM of NLB resistance QTL NLB_6.01, which NLB resistance QTL NLB_3.01 is located in a chromosomal interval flanked by any two of the marker loci selected from the group consisting of SEQ ID NOs: 74 to 82.
[0120] In an aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a marker locus associated with NLB resistance QTL NLB_7.01, which NLB resistance QTL NLB_7.01 is located in a chromosomal interval flanked by the marker loci SEQ ID NOs: 469 and 470.
[0121] In an aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a marker locus associated with and within about 20 cM of NLB resistance QTL NLB_7.01, which NLB resistance QTL NLB_7.01 is located in a chromosomal interval flanked by any two of the marker loci selected from the group consisting of SEQ ID NOs: 469 and 470. In an aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a marker locus associated with and within about 15 cM of NLB resistance QTL NLB_7.01, which NLB resistance QTL NLB_7.01 is located in a chromosomal interval flanked by any two of the marker loci selected from the group consisting of SEQ ID NOs: 469 and 470. In an aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a marker locus associated with and within about 10 cM of NLB resistance QTL NLB_7.01, which NLB resistance QTL NLB_7.01 is located in a chromosomal interval flanked by any two of the marker loci selected from the group consisting of SEQ ID NOs: 469 and 470. In an aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a marker locus associated with and within about 5 cM of NLB resistance QTL NLB_7.01, which NLB resistance QTL NLB_7.01 is located in a chromosomal interval flanked by any two of the marker loci selected from the group consisting of SEQ ID NOs: 469 and 470. In an aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a marker locus associated with and within about 4 cM of NLB resistance QTL NLB_7.01, which NLB resistance QTL NLB_7.01 is located in a chromosomal interval flanked by any two of the marker loci selected from the group consisting of SEQ ID NOs: 469 and 470. In an aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a marker locus associated with and within about 3 cM of NLB resistance QTL NLB_7.01, which NLB resistance QTL NLB_7.01 is located in a chromosomal interval flanked by any two of the marker loci selected from the group consisting of SEQ ID NOs: 469 and 470. In an aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a marker locus associated with and within about 2 cM of NLB resistance QTL NLB_7.01, which NLB resistance QTL NLB_7.01 is located in a chromosomal interval flanked by any two of the marker loci selected from the group consisting of SEQ ID NOs: 469 and 470. In an aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a marker locus associated with and within about 1 cM of NLB resistance QTL NLB_7.01, which NLB resistance QTL NLB_7.01 is located in a chromosomal interval flanked by any two of the marker loci selected from the group consisting of SEQ ID NOs: 469 and 470. In an aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a marker locus associated with and within about 0.5 cM of NLB resistance QTL NLB_7.01, which NLB resistance QTL NLB_7.01 is located in a chromosomal interval flanked by any two of the marker loci selected from the group consisting of SEQ ID NOs: 469 and 470. In an aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a marker locus within less than about 0.5 cM of NLB resistance QTL NLB_7.01, which NLB resistance QTL NLB_3.01 is located in a chromosomal interval flanked by any two of the marker loci selected from the group consisting of SEQ ID NOs: 469 and 470.
[0122] In an aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a marker locus associated with NLB resistance QTL NLB_9.01, which NLB resistance QTL NLB_9.01 is located in a chromosomal interval flanked by any two of the marker loci selected from the group consisting of SEQ ID NOs: 85 to 89 and 476 to 482. In another aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a marker locus associated with NLB resistance QTL NLB_9.01, which NLB resistance QTL NLB_9.01 is located in a chromosomal interval flanked by any two of the marker loci selected from the group consisting of SEQ ID NOs: 86 to 89, 476, 477, 479, and 480. In another aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a marker locus associated with NLB resistance QTL NLB_9.01, which NLB resistance QTL NLB_9.01 is located in a chromosomal interval flanked by any two of the marker loci selected from the group consisting of SEQ ID NOs: 87 to 89, 477, and 480.
[0123] In an aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a marker locus associated with and within about 20 cM of NLB resistance QTL NLB_9.01, which NLB resistance QTL NLB_9.01 is located in a chromosomal interval flanked by any two of the marker loci selected from the group consisting of SEQ ID NOs: 85 to 89 and 476 to 482. In an aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a marker locus associated with and within about 15 cM of NLB resistance QTL NLB_9.01, which NLB resistance QTL NLB_9.01 is located in a chromosomal interval flanked by any two of the marker loci selected from the group consisting of SEQ ID NOs: 85 to 89 and 476 to 482. In an aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a marker locus associated with and within about 10 cM of NLB resistance QTL NLB_9.01, which NLB resistance QTL NLB_9.01 is located in a chromosomal interval flanked by any two of the marker loci selected from the group consisting of SEQ ID NOs: 85 to 89 and 476 to 482. In an aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a marker locus associated with and within about 5 cM of NLB resistance QTL NLB_9.01, which NLB resistance QTL NLB_9.01 is located in a chromosomal interval flanked by any two of the marker loci selected from the group consisting of SEQ ID NOs: 85 to 89 and 476 to 482. In an aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a marker locus associated with and within about 4 cM of NLB resistance QTL NLB_9.01, which NLB resistance QTL NLB_9.01 is located in a chromosomal interval flanked by any two of the marker loci selected from the group consisting of SEQ ID NOs: 85 to 89 and 476 to 482. In an aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a marker locus associated with and within about 3 cM of NLB resistance QTL NLB_9.01, which NLB resistance QTL NLB_9.01 is located in a chromosomal interval flanked by any two of the marker loci selected from the group consisting of SEQ ID NOs: 85 to 89 and 476 to 482. In an aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a marker locus associated with and within about 2 cM of NLB resistance QTL NLB_9.01, which NLB resistance QTL NLB_9.01 is located in a chromosomal interval flanked by any two of the marker loci selected from the group consisting of SEQ ID NOs: 85 to 89 and 476 to 482. In an aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a marker locus associated with and within about 1 cM of NLB resistance QTL NLB_9.01, which NLB resistance QTL NLB_9.01 is located in a chromosomal interval flanked by any two of the marker loci selected from the group consisting of SEQ ID NOs: 85 to 89 and 476 to 482. In an aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a marker locus associated with and within about 0.5 cM of NLB resistance QTL NLB_9.01, which NLB resistance QTL NLB_9.01 is located in a chromosomal interval flanked by any two of the marker loci selected from the group consisting of SEQ ID NOs: 85 to 89 and 476 to 482. In an aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a marker locus within less than about 0.5 cM of NLB resistance QTL NLB_9.01, which NLB resistance QTL NLB_3.01 is located in a chromosomal interval flanked by any two of the marker loci selected from the group consisting of SEQ ID NOs: 85 to 89 and 476 to 482.
[0124] In a further aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a marker locus located in a chromosomal interval flanked by any two of marker loci SEQ ID NOs: 1 to 18. In another aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a marker locus located in a chromosomal interval flanked by any two of marker loci SEQ ID NOs: 12 to 15. In another aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a marker locus located in a chromosomal interval flanked by any two of marker loci SEQ ID NOs: 8 to 18.
[0125] In a further aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a marker locus located in a chromosomal interval flanked by any two of marker loci SEQ ID NOs: 19 to 31. In another aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a marker locus located in a chromosomal interval flanked by any two of marker loci SEQ ID NOs: 22 to 25. In another aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a marker locus located in a chromosomal interval flanked by any two of marker loci SEQ ID NOs: 21 to 29.
[0126] In a further aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a marker locus located in a chromosomal interval flanked by any two of marker loci SEQ ID NOs: 32 to 52 and 471 to 475. In another aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a marker locus located in a chromosomal interval flanked by any two of marker loci SEQ ID NOs: 37 to 42 and 474. In yet another aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a marker locus located in a chromosomal interval flanked by any two of marker loci SEQ ID NOs: 44 to 49. In yet another aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a marker locus located in a chromosomal interval flanked by any two of marker loci SEQ ID NOs: 33 to 42, 473, and 474. In yet another aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a marker locus located in a chromosomal interval flanked by any two of marker loci SEQ ID NOs: 43 to 49 and 475.
[0127] In a further aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a marker locus located in a chromosomal interval flanked by any two of marker loci SEQ ID NOs: 53 to 65 and 446 to 468. In another aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a marker locus located in a chromosomal interval flanked by any two of marker loci SEQ ID NOs: 57 to 62 and 458 to 466. In another aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a marker locus located in a chromosomal interval flanked by any two of marker loci SEQ ID NOs: 57 to 64 and 458 to 468.
[0128] In a further aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a marker locus located in a chromosomal interval flanked by any two of marker loci SEQ ID NOs: 66 to 84. In another aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a marker locus located in a chromosomal interval flanked by any two of marker loci SEQ ID NOs: 79 to 81. In another aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a marker locus located in a chromosomal interval flanked by any two of marker loci SEQ ID NOs: 74 to 82.
[0129] In a further aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a marker locus located in a chromosomal interval flanked by marker loci SEQ ID NOs: 469 and 470.
[0130] In a further aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a marker locus located in a chromosomal interval flanked by any two of marker loci SEQ ID NOs: 85 to 89 and 476 to 482. In another aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a marker locus located in a chromosomal interval flanked by any two of marker loci SEQ ID NOs: 86 to 89, 476, 477, 479, and 480. In another aspect, a method provided herein comprises genotyping a corn plant, seed, or cell at a marker locus located in a chromosomal interval flanked by any two of marker loci SEQ ID NOs: 87 to 89, 477, and 480.
[0131] In another aspect, a method provided herein comprises genotyping a corn plant, seed, or cell by detecting a haplotype. In an aspect, a haplotype comprises an NLB resistance allele at one or more, two or more, three or more, four or more, or five or more of marker loci SEQ ID NOs: 1 to 18. In an aspect, a haplotype comprises an NLB resistance allele at two or more of marker loci SEQ ID NOs: 1 to 18. In an aspect, a haplotype comprises an NLB resistance allele at three or more of marker loci SEQ ID NOs: 1 to 18. In an aspect, a haplotype comprises an NLB resistance allele at four or more of marker loci SEQ ID NOs: 1 to 18. In an aspect, a haplotype comprises an NLB resistance allele at five or more of marker loci SEQ ID NOs: 1 to 18. In an aspect, a haplotype comprises an NLB resistance allele at one or more of marker loci SEQ ID NOs: 12 to 15. In an aspect, a haplotype comprises an NLB resistance allele at two or more of marker loci SEQ ID NOs: 12 to 15. In an aspect, a haplotype comprises an NLB resistance allele at three or more of marker loci SEQ ID NOs: 12 to 15. In an aspect, a haplotype comprises an NLB resistance allele at marker loci SEQ ID NOs: 12 to 15. In an aspect, a haplotype comprises an NLB resistance allele at one or more of marker loci SEQ ID NO: 8 to 18. In an aspect, a haplotype comprises an NLB resistance allele at two or more of marker loci SEQ ID NOs: 8 to 18. In an aspect, a haplotype comprises an NLB resistance allele at three or more of marker loci SEQ ID NOs: 8 to 18. In an aspect, a haplotype comprises an NLB resistance allele at four or more of marker loci SEQ ID NOs: 8 to 18. In an aspect, a haplotype comprises an NLB resistance allele at five or more of marker loci SEQ ID NOs: 8 to 18.
[0132] In an aspect, a haplotype comprises an NLB resistance allele at one or more, two or more, three or more, four or more, or five or more of marker loci SEQ ID NOs: 19 to 31. In an aspect, a haplotype comprises an NLB resistance allele at two or more of marker loci SEQ ID NOs: 19 to 31. In an aspect, a haplotype comprises an NLB resistance allele at three or more of marker loci SEQ ID NOs: 19 to 31. In an aspect, a haplotype comprises an NLB resistance allele at four or more of marker loci SEQ ID NOs: 19 to 31. In an aspect, a haplotype comprises an NLB resistance allele at five or more of marker loci SEQ ID NOs: 19 to 31. In an aspect, a haplotype comprises an NLB resistance allele at one or more of marker loci SEQ ID NO: 22 to 25. In an aspect, a haplotype comprises an NLB resistance allele at two or more of marker loci SEQ ID NOs: 22 to 25. In an aspect, a haplotype comprises an NLB resistance allele at three or more of marker loci SEQ ID NOs: 22 to 25. In an aspect, a haplotype comprises an NLB resistance allele at marker loci SEQ ID NOs: 22 to 25. In an aspect, a haplotype comprises an NLB resistance allele at one or more of marker loci SEQ ID NO: 21 to 29. In an aspect, a haplotype comprises an NLB resistance allele at two or more of marker loci SEQ ID NOs: 21 to 29. In an aspect, a haplotype comprises an NLB resistance allele at three or more of marker loci SEQ ID NOs: 21 to 29. In an aspect, a haplotype comprises an NLB resistance allele at four or more of marker loci SEQ ID NOs: 21 to 29. In an aspect, a haplotype comprises an NLB resistance allele at five or more of marker loci SEQ ID NOs: 21 to 29.
[0133] In an aspect, a haplotype comprises an NLB resistance allele at one or more of marker loci SEQ ID NO: 32 to 52 and 471 to 475. In an aspect, a haplotype comprises an NLB resistance allele at two or more of marker loci SEQ ID NOs: 32 to 52 and 471 to 475. In an aspect, a haplotype comprises an NLB resistance allele at three or more of marker loci SEQ ID NOs: 32 to 52 and 471 to 475. In an aspect, a haplotype comprises an NLB resistance allele at four or more of marker loci SEQ ID NOs: 32 to 52 and 471 to 475. In an aspect, a haplotype comprises an NLB resistance allele at five or more of marker loci SEQ ID NOs: 32 to 52 and 471 to 475. In an aspect, a haplotype comprises an NLB resistance allele at one or more of marker loci SEQ ID NO: 37 to 42 and 474. In an aspect, a haplotype comprises an NLB resistance allele at two or more of marker loci SEQ ID NOs: 37 to 42 and 474. In an aspect, a haplotype comprises an NLB resistance allele at three or more of marker loci SEQ ID NOs: 37 to 42 and 474. In an aspect, a haplotype comprises an NLB resistance allele at four or more of marker loci SEQ ID NOs: 37 to 42 and 474. In an aspect, a haplotype comprises an NLB resistance allele at five or more of marker loci SEQ ID NOs: 37 to 42 and 474. In an aspect, a haplotype comprises an NLB resistance allele at one or more of marker loci SEQ ID NO: 33 to 42, 473 and 474. In an aspect, a haplotype comprises an NLB resistance allele at two or more of marker loci SEQ ID NOs: 33 to 42, 473 and 474. In an aspect, a haplotype comprises an NLB resistance allele at three or more of marker loci SEQ ID NOs: 33 to 42, 473 and 474. In an aspect, a haplotype comprises an NLB resistance allele at four or more of marker loci SEQ ID NOs: 33 to 42, 473 and 474. In an aspect, a haplotype comprises an NLB resistance allele at five or more of marker loci SEQ ID NOs: 33 to 42, 473 and 474. In an aspect, a haplotype comprises an NLB resistance allele at one or more of marker loci SEQ ID NO: 43 to 49 and 475. In an aspect, a haplotype comprises an NLB resistance allele at two or more of marker loci SEQ ID NOs: 43 to 49 and 475. In an aspect, a haplotype comprises an NLB resistance allele at three or more of marker loci SEQ ID NOs: 43 to 49 and 475. In an aspect, a haplotype comprises an NLB resistance allele at four or more of marker loci SEQ ID NOs: 43 to 49 and 475. In an aspect, a haplotype comprises an NLB resistance allele at five or more of marker loci SEQ ID NOs: 43 to 49 and 475. In an aspect, a haplotype comprises an NLB resistance allele at one or more of marker loci SEQ ID NO: 44 to 49. In an aspect, a haplotype comprises an NLB resistance allele at two or more of marker loci SEQ ID NOs: 44 to 49. In an aspect, a haplotype comprises an NLB resistance allele at three or more of marker loci SEQ ID NOs: 44 to 49. In an aspect, a haplotype comprises an NLB resistance allele at four or more of marker loci SEQ ID NOs: 44 to 49. In an aspect, a haplotype comprises an NLB resistance allele at five or more of marker loci SEQ ID NOs: 44 to 49.
[0134] In an aspect, a haplotype comprises an NLB resistance allele at one or more of marker loci SEQ ID NO: 53 to 65 and 446 to 468. In an aspect, a haplotype comprises an NLB resistance allele at two or more of marker loci SEQ ID NOs: 53 to 65 and 446 to 468. In an aspect, a haplotype comprises an NLB resistance allele at three or more of marker loci SEQ ID NOs: 53 to 65 and 446 to 468. In an aspect, a haplotype comprises an NLB resistance allele at four or more of marker loci SEQ ID NOs: 53 to 65 and 446 to 468. In an aspect, a haplotype comprises an NLB resistance allele at five or more of marker loci SEQ ID NOs: 53 to 65 and 446 to 468. In an aspect, a haplotype comprises an NLB resistance allele at one or more of marker loci SEQ ID NO: 57 to 64 and 458 to 468. In an aspect, a haplotype comprises an NLB resistance allele at two or more of marker loci SEQ ID NOs: 57 to 64 and 458 to 468. In an aspect, a haplotype comprises an NLB resistance allele at three or more of marker loci SEQ ID NOs: 57 to 64 and 458 to 468. In an aspect, a haplotype comprises an NLB resistance allele at four or more of marker loci SEQ ID NOs: 57 to 64 and 458 to 468. In an aspect, a haplotype comprises an NLB resistance allele at five or more of marker loci SEQ ID NOs: 57 to 64 and 458 to 468. In an aspect, a haplotype comprises an NLB resistance allele at one or more of marker loci SEQ ID NO: 57 to 62 and 458 to 466. In an aspect, a haplotype comprises an NLB resistance allele at two or more of marker loci SEQ ID NOs: 57 to 62 and 458 to 466. In an aspect, a haplotype comprises an NLB resistance allele at three or more of marker loci SEQ ID NOs: 57 to 62 and 458 to 466. In an aspect, a haplotype comprises an NLB resistance allele at four or more of marker loci SEQ ID NOs: 57 to 62 and 458 to 466. In an aspect, a haplotype comprises an NLB resistance allele at five or more of marker loci SEQ ID NOs: 57 to 62 and 458 to 466.
[0135] In an aspect, a haplotype comprises an NLB resistance allele at one or more of marker loci SEQ ID NO: 66 to 84. In an aspect, a haplotype comprises an NLB resistance allele at two or more of marker loci SEQ ID NOs: 66 to 84. In an aspect, a haplotype comprises an NLB resistance allele at three or more of marker loci SEQ ID NOs: 66 to 84. In an aspect, a haplotype comprises an NLB resistance allele at four or more of marker loci SEQ ID NOs: 66 to 84. In an aspect, a haplotype comprises an NLB resistance allele at five or more of marker loci SEQ ID NOs: 66 to 84. In an aspect, a haplotype comprises an NLB resistance allele at one or more of marker loci SEQ ID NO: 79 to 81. In an aspect, a haplotype comprises an NLB resistance allele at two or more of marker loci SEQ ID NOs: 79 to 81. In an aspect, a haplotype comprises an NLB resistance allele at marker loci SEQ ID NOs: 79 to 81. In an aspect, a haplotype comprises an NLB resistance allele at one or more of marker loci SEQ ID NO: 74 to 82. In an aspect, a haplotype comprises an NLB resistance allele at two or more of marker loci SEQ ID NOs: 74 to 82. In an aspect, a haplotype comprises an NLB resistance allele at three or more of marker loci SEQ ID NOs: 74 to 82. In an aspect, a haplotype comprises an NLB resistance allele at four or more of marker loci SEQ ID NOs: 74 to 82. In an aspect, a haplotype comprises an NLB resistance allele at five or more of marker loci SEQ ID NOs: 74 to 82.
[0136] In an aspect, a haplotype comprises an NLB resistance allele at one or more of marker loci SEQ ID NO: 85 to 89 and 476 to 482. In an aspect, a haplotype comprises an NLB resistance allele at two or more of marker loci SEQ ID NOs: 85 to 89 and 476 to 482. In an aspect, a haplotype comprises an NLB resistance allele at three or more of marker loci SEQ ID NOs: 85 to 89 and 476 to 482. In an aspect, a haplotype comprises an NLB resistance allele at four or more of marker loci SEQ ID NOs: 85 to 89 and 476 to 482. In an aspect, a haplotype comprises an NLB resistance allele at five or more of marker loci SEQ ID NOs: 85 to 89 and 476 to 482. In an aspect, a haplotype comprises an NLB resistance allele at one or more of marker loci SEQ ID NO: 86 to 89, 476, 477, 479, and 480. In an aspect, a haplotype comprises an NLB resistance allele at two or more of marker loci SEQ ID NOs: 86 to 89, 476, 477, 479, and 480. In an aspect, a haplotype comprises an NLB resistance allele at three or more of marker loci SEQ ID NOs: 86 to 89, 476, 477, 479, and 480. In an aspect, a haplotype comprises an NLB resistance allele at four or more of marker loci SEQ ID NOs: 86 to 89, 476, 477, 479, and 480. In an aspect, a haplotype comprises an NLB resistance allele at five or more of marker loci SEQ ID NOs: 86 to 89, 476, 477, 479, and 480. In an aspect, a haplotype comprises an NLB resistance allele at one or more, or two or more of marker loci SEQ ID NO: 87 to 89, 477 and 480. In an aspect, a haplotype comprises an NLB resistance allele at two or more of marker loci SEQ ID NOs: 87 to 89, 477 and 480. In an aspect, a haplotype comprises an NLB resistance allele at three or more of marker loci SEQ ID NOs: 87 to 89, 477 and 480. In an aspect, a haplotype comprises an NLB resistance allele at four or more of marker loci SEQ ID NOs: 87 to 89, 477 and 480. In an aspect, a haplotype comprises an NLB resistance allele at five or more of marker loci SEQ ID NOs: 87 to 89, 477 and 480.
[0137] In an aspect, a haplotype comprises an NLB resistance allele at one or more of marker loci SEQ ID NO: 469 and 470. In an aspect, a haplotype comprises an NLB resistance allele at marker loci SEQ ID NO: 469 and 470.
[0138] In an aspect, a corn plant, seed, or cell comprising NLB resistance QTLs or NLB resistant alleles provided herein exhibits intermediate resistance to NLB infection from Exserohilum turcicum (also referred to as Helminthosporium turcicum or Setosphaeria turcica). In another aspect, a corn plant, seed, or cell comprising NLB resistance QTLs or NLB resistant alleles provided herein exhibits at least mild resistance (e.g., NLB resistance score of <5; see Table 1) to NLB infection from Exserohilum turcicum (also referred to as Helminthosporium turcicum or Setosphaeria turcica). In a further aspect, a corn plant, seed, or cell comprising NLB resistance QTLs or NLB resistant alleles provided herein exhibits resistance (e.g., NLB resistance score of <4; see Table 1) to NLB infection from Exserohilum turcicum (also referred to as Helminthosporium turcicum or Setosphaeria turcica). In an aspect, NLB infection is caused by Exserohilum turcicum (also referred to as Helminthosporium turcicum or Setosphaeria turcica).
[0139] As used herein, a "low NLB stress condition" refers to a condition where very few to no NLB susceptible corn plants in a field plot (e.g., less than 10%) exhibit signs of NLB infection. Signs of NLB infection can include: leaf lesions, foliage destruction, root rot, or stalk rot.
[0140] As used herein, a "high NLB stress condition" refers to a condition where a plurality of NLB susceptible corn plants in a field plot (e.g., more than 30%) exhibit signs of NLB infection.
[0141] As an example, an NLB resistance QTL or NLB resistance allele provided herein does not confer a yield penalty under a low NLB stress condition. In another example, a combination of two or more, three or more, four or more, five or more, or six or more NLB resistance QTLs provided herein does not confer a yield penalty under a low NLB stress condition. In an aspect, presence of NLB resistance QTL NLB_2.01 in a corn plant, seed, or cell genome does not confer a yield penalty under a low NLB stress condition. In an aspect, presence of NLB resistance QTL NLB_3.01 in a corn plant, seed, or cell genome does not confer a yield penalty under a low NLB stress condition. In an aspect, presence of NLB resistance QTL NLB_4.01 in a corn plant, seed, or cell genome does not confer a yield penalty under a low NLB stress condition. In an aspect, presence of NLB resistance QTL NLB_4.02 in a corn plant, seed, or cell genome does not confer a yield penalty under a low NLB stress condition. In an aspect, presence of NLB resistance QTL NLB_5.01 in a corn plant, seed, or cell genome does not confer a yield penalty under a low NLB stress condition. In an aspect, presence of NLB resistance QTL NLB_6.01 in a corn plant, seed, or cell genome does not confer a yield penalty under a low NLB stress condition. In an aspect, presence of NLB resistance QTL NLB_7.01 in a corn plant, seed, or cell genome does not confer a yield penalty under a low NLB stress condition. In an aspect, presence of NLB resistance QTL NLB_9.01 in a corn plant, seed, or cell genome does not confer a yield penalty under a low NLB stress condition.
[0142] In another aspect, a corn plant or seed provided herein comprising one or more, two or more, three or more, four or more, five or more, six or more, or seven or more NLB resistance QTLs selected from the group consisting of NLB resistance QTLs NLB_2.01, NLB_3.01, NLB_4.01, NLB_4.02, NLB_5.01, NLB_6.01, NLB_7.01, and NLB_9.01 exhibits a reduction of NLB rating score of about 0.25 or more, 0.5 or more, 0.75 or more, 1 or more, 1.5 or more, 2 or more, 2.5 or more, 3 or more, 3.5 or more, 4 or more, 4.5 or more, 5 or more, 5.5 or more, 6 or more, 6.5 or more, 7 or more, or 7.5 or more compared to a corn plant or seed without the one or more, two or more, three or more, four or more, five or more, or six or more NLB resistance QTLs under a high NLB stress condition.
[0143] In an aspect, a corn plant or seed provided herein as described in any of paragraphs [00175] to [00183] exhibits a reduction of NLB rating score of about 0.25 or more compared to a corn plant without the one or more, two or more, three or more, four or more, five or more, six or more, or seven or more NLB resistance QTLs under a high NLB stress condition. In an aspect, a corn plant or seed provided herein as described in any of paragraphs [00175] to [00183] exhibits a reduction of NLB rating score of about 0.5 or more compared to a corn plant without the one or more, two or more, three or more, four or more, five or more, six or more, or seven or more NLB resistance QTLs under a high NLB stress condition. In an aspect, a corn plant or seed provided herein as described in any of paragraphs [00175] to [00183] exhibits a reduction of NLB rating score of about 0.75 or more compared to a corn plant without the one or more, two or more, three or more, four or more, five or more, six or more, or seven or more NLB resistance QTLs under a high NLB stress condition. In an aspect, a corn plant or seed provided herein as described in any of paragraphs [00175] to [00183] exhibits a reduction of NLB rating score of about 1 or more compared to a corn plant without the one or more, two or more, three or more, four or more, five or more, six or more, or seven or more NLB resistance QTLs under a high NLB stress condition. In an aspect, a corn plant or seed provided herein as described in any of paragraphs [00175] to [00183] exhibits a reduction of NLB rating score of about 1.5 or more compared to a corn plant without the one or more, two or more, three or more, four or more, five or more, six or more, or seven or more NLB resistance QTLs under a high NLB stress condition. In an aspect, a corn plant or seed provided herein as described in any of paragraphs [00175] to [00183] exhibits a reduction of NLB rating score of about 2 or more compared to a corn plant without the one or more, two or more, three or more, four or more, five or more, six or more, or seven or more NLB resistance QTLs under a high NLB stress condition. In an aspect, a corn plant or seed provided herein as described in any of paragraphs [00175] to [00183] exhibits a reduction of NLB rating score of about 2.5 or more compared to a corn plant without the one or more, two or more, three or more, four or more, five or more, six or more, or seven or more NLB resistance QTLs under a high NLB stress condition. In an aspect, a corn plant or seed provided herein as described in any of paragraphs [00175] to [00183] exhibits a reduction of NLB rating score of about 3 or more compared to a corn plant without the one or more, two or more, three or more, four or more, five or more, six or more, or seven or more NLB resistance QTLs under a high NLB stress condition. In an aspect, a corn plant or seed provided herein as described in any of paragraphs [00175] to [00183] exhibits a reduction of NLB rating score of about 3.5 or more compared to a corn plant without the one or more, two or more, three or more, four or more, five or more, six or more, or seven or more NLB resistance QTLs under a high NLB stress condition. In an aspect, a corn plant or seed provided herein as described in any of paragraphs [00175] to [00183] exhibits a reduction of NLB rating score of about 4 or more compared to a corn plant without the one or more, two or more, three or more, four or more, five or more, six or more, or seven or more NLB resistance QTLs under a high NLB stress condition. In an aspect, a corn plant or seed provided herein as described in any of paragraphs [00175] to [00183] exhibits a reduction of NLB rating score of about 4.5 or more compared to a corn plant without the one or more, two or more, three or more, four or more, five or more, six or more, or seven or more NLB resistance QTLs under a high NLB stress condition. In an aspect, a corn plant or seed provided herein as described in any of paragraphs [00175] to [00183] exhibits a reduction of NLB rating score of about 5 or more compared to a corn plant without the one or more, two or more, three or more, four or more, five or more, six or more, or seven or more NLB resistance QTLs under a high NLB stress condition. In an aspect, a corn plant or seed provided herein as described in any of paragraphs [00175] to [00183] exhibits a reduction of NLB rating score of about 5.5 or more compared to a corn plant without the one or more, two or more, three or more, four or more, five or more, six or more, or seven or more NLB resistance QTLs under a high NLB stress condition. In an aspect, a corn plant or seed provided herein as described in any of paragraphs [00175] to [00183] exhibits a reduction of NLB rating score of about 6 or more compared to a corn plant without the one or more, two or more, three or more, four or more, five or more, six or more, or seven or more NLB resistance QTLs under a high NLB stress condition. In an aspect, a corn plant or seed provided herein as described in any of paragraphs [00175] to [00183] exhibits a reduction of NLB rating score of about 6.5 or more compared to a corn plant without the one or more, two or more, three or more, four or more, five or more, six or more, or seven or more NLB resistance QTLs under a high NLB stress condition. In an aspect, a corn plant or seed provided herein as described in any of paragraphs [00175] to [00183] exhibits a reduction of NLB rating score of about 7 or more compared to a corn plant without the one or more, two or more, three or more, four or more, five or more, six or more, or seven or more NLB resistance QTLs under a high NLB stress condition. In an aspect, a corn plant or seed provided herein as described in any of paragraphs [00175] to [00183] exhibits a reduction of NLB rating score of about 7.5 or more compared to a corn plant without the one or more, two or more, three or more, four or more, five or more, six or more, or seven or more NLB resistance QTLs under a high NLB stress condition.
[0144] In another aspect, a corn plant or seed provided herein comprising one or more, two or more, three or more, four or more, five or more, six or more, or seven or more NLB resistance QTLs or NLB resistance alleles provided herein exhibits a reduction of NLB rating score of about 0.25 or more, 0.5 or more, 0.75 or more, 1 or more, 1.5 or more, 2 or more, 2.5 or more, 3 or more, 3.5 or more, 4 or more, 4.5 or more, 5 or more, 5.5 or more, 6 or more, 6.5 or more, 7 or more, or 7.5 or more compared to a corn plant or seed without the one or more, two or more, three or more, four or more, five or more, or six or more NLB resistance QTLs or NLB resistance alleles under a high NLB stress condition.
[0145] In another aspect, a corn plant or seed provided herein comprising one or more, two or more, three or more, four or more, five or more, six or more, or seven or more NLB resistance QTLs selected from the group consisting of NLB resistance QTLs NLB_2.01, NLB_3.01, NLB_4.01, NLB_4.02, NLB_5.01, NLB_6.01, NLB_7.01, and NLB_9.01 exhibits a reduction of NLB rating score of between 0.25 and 8, between 0.25 and 7.5, between 0.25 and 7, between 0.25 and 6.5, between 0.25 and 6, between 0.25 and 5.5, between 0.25 and 5, between 0.25 and 4.5, between 0.25 and 4, between 0.25 and 3.5, between 0.25 and 3, between 0.25 and 2.5, between 0.25 and 2, between 0.25 and 1.5, between 0.25 and 1, between 1 and 8, between 1 and 7, between 1 and 6, between 1 and 5, between 1 and 4, between 1 and 3, or between 1 and 2 compared to a corn plant or seed without the one or more, two or more, three or more, four or more, five or more, six or more, or seven or more NLB resistance QTLs under a high NLB stress condition.
[0146] In an aspect, a corn plant or seed provided herein as described in any of paragraphs [00175] to [00183] exhibits a reduction of NLB rating score of between 0.25 and 8 compared to a corn plant without the one or more, two or more, three or more, four or more, five or more, six or more, or seven or more NLB resistance QTLs under a high NLB stress condition. In an aspect, a corn plant or seed provided herein as described in any of paragraphs [00175] to [00183] exhibits a reduction of NLB rating score of between 0.25 and 7.5 compared to a corn plant without the one or more, two or more, three or more, four or more, five or more, six or more, or seven or more NLB resistance QTLs under a high NLB stress condition. In an aspect, a corn plant or seed provided herein as described in any of paragraphs [00175] to [00183] exhibits a reduction of NLB rating score of between 0.25 and 7 compared to a corn plant without the one or more, two or more, three or more, four or more, five or more, six or more, or seven or more NLB resistance QTLs under a high NLB stress condition. In an aspect, a corn plant or seed provided herein as described in any of paragraphs [00175] to [00183] exhibits a reduction of NLB rating score of between 0.25 and 6.5 compared to a corn plant without the one or more, two or more, three or more, four or more, five or more, six or more, or seven or more NLB resistance QTLs under a high NLB stress condition. In an aspect, a corn plant or seed provided herein as described in any of paragraphs [00175] to [00183] exhibits a reduction of NLB rating score of between 0.25 and 6 compared to a corn plant without the one or more, two or more, three or more, four or more, five or more, six or more, or seven or more NLB resistance QTLs under a high NLB stress condition. In an aspect, a corn plant or seed provided herein as described in any of paragraphs [00175] to [00183] exhibits a reduction of NLB rating score of between 0.25 and 5.5 compared to a corn plant without the one or more, two or more, three or more, four or more, five or more, six or more, or seven or more NLB resistance QTLs under a high NLB stress condition. In an aspect, a corn plant or seed provided herein as described in any of paragraphs [00175] to [00183] exhibits a reduction of NLB rating score of between 0.25 and 5 compared to a corn plant without the one or more, two or more, three or more, four or more, five or more, six or more, or seven or more NLB resistance QTLs under a high NLB stress condition. In an aspect, a corn plant or seed provided herein as described in any of paragraphs [00175] to [00183] exhibits a reduction of NLB rating score of between 0.25 and 4.5 compared to a corn plant without the one or more, two or more, three or more, four or more, five or more, six or more, or seven or more NLB resistance QTLs under a high NLB stress condition. In an aspect, a corn plant or seed provided herein as described in any of paragraphs [00175] to [00183] exhibits a reduction of NLB rating score of between 0.25 and 4 compared to a corn plant without the one or more, two or more, three or more, four or more, five or more, six or more, or seven or more NLB resistance QTLs under a high NLB stress condition. In an aspect, a corn plant or seed provided herein as described in any of paragraphs [00175] to [00183] exhibits a reduction of NLB rating score of between 0.25 and 3.5 compared to a corn plant without the one or more, two or more, three or more, four or more, five or more, six or more, or seven or more NLB resistance QTLs under a high NLB stress condition. In an aspect, a corn plant or seed provided herein as described in any of paragraphs [00175] to [00183] exhibits a reduction of NLB rating score of between 0.25 and 3 compared to a corn plant without the one or more, two or more, three or more, four or more, five or more, six or more, or seven or more NLB resistance QTLs under a high NLB stress condition. In an aspect, a corn plant or seed provided herein as described in any of paragraphs [00175] to [00183] exhibits a reduction of NLB rating score of between 0.25 and 2.5 compared to a corn plant without the one or more, two or more, three or more, four or more, five or more, six or more, or seven or more NLB resistance QTLs under a high NLB stress condition. In an aspect, a corn plant or seed provided herein as described in any of paragraphs [00175] to [00183] exhibits a reduction of NLB rating score of between 0.25 and 2 compared to a corn plant without the one or more, two or more, three or more, four or more, five or more, six or more, or seven or more NLB resistance QTLs under a high NLB stress condition. In an aspect, a corn plant or seed provided herein as described in any of paragraphs [00175] to [00183] exhibits a reduction of NLB rating score of between 0.25 and 1.5 compared to a corn plant without the one or more, two or more, three or more, four or more, five or more, six or more, or seven or more NLB resistance QTLs under a high NLB stress condition. In an aspect, a corn plant or seed provided herein as described in any of paragraphs [00175] to [00183] exhibits a reduction of NLB rating score of between 0.25 and 1 compared to a corn plant without the one or more, two or more, three or more, four or more, five or more, six or more, or seven or more NLB resistance QTLs under a high NLB stress condition. In an aspect, a corn plant or seed provided herein as described in any of paragraphs [00175] to [00183] exhibits a reduction of NLB rating score of between 1 and 8 compared to a corn plant without the one or more, two or more, three or more, four or more, five or more, six or more, or seven or more NLB resistance QTLs under a high NLB stress condition. In an aspect, a corn plant or seed provided herein as described in any of paragraphs [00175] to [00183] exhibits a reduction of NLB rating score of between 1 and 7 compared to a corn plant without the one or more, two or more, three or more, four or more, five or more, six or more, or seven or more NLB resistance QTLs under a high NLB stress condition. In an aspect, a corn plant or seed provided herein as described in any of paragraphs [00175] to [00183] exhibits a reduction of NLB rating score of between 1 and 6 compared to a corn plant without the one or more, two or more, three or more, four or more, five or more, six or more, or seven or more NLB resistance QTLs under a high NLB stress condition. In an aspect, a corn plant or seed provided herein as described in any of paragraphs [00175] to [00183] exhibits a reduction of NLB rating score of between 1 and 5 compared to a corn plant without the one or more, two or more, three or more, four or more, five or more, six or more, or seven or more NLB resistance QTLs under a high NLB stress condition. In an aspect, a corn plant or seed provided herein as described in any of paragraphs [00175] to [00183] exhibits a reduction of NLB rating score of between 1 and 4 compared to a corn plant without the one or more, two or more, three or more, four or more, five or more, six or more, or seven or more NLB resistance QTLs under a high NLB stress condition. In an aspect, a corn plant or seed provided herein as described in any of paragraphs [00175] to [00183] exhibits a reduction of NLB rating score of between 1 and 3 compared to a corn plant without the one or more, two or more, three or more, four or more, five or more, six or more, or seven or more NLB resistance QTLs under a high NLB stress condition. In an aspect, a corn plant or seed provided herein as described in any of paragraphs [00175] to [00183] exhibits a reduction of NLB rating score of between 1 and 2 compared to a corn plant without the one or more, two or more, three or more, four or more, five or more, six or more, or seven or more NLB resistance QTLs under a high NLB stress condition.
[0147] In another aspect, a corn plant or seed provided herein comprising one or more, two or more, three or more, four or more, five or more, six or more, or seven or more NLB resistance QTLs or NLB resistance alleles provided herein exhibits a reduction of NLB rating score of between 0.25 and 8, between 0.25 and 7.5, between 0.25 and 7, between 0.25 and 6.5, between 0.25 and 6, between 0.25 and 5.5, between 0.25 and 5, between 0.25 and 4.5, between 0.25 and 4, between 0.25 and 3.5, between 0.25 and 3, between 0.25 and 2.5, between 0.25 and 2, between 0.25 and 1.5, between 0.25 and 1, between 1 and 8, between 1 and 7, between 1 and 6, between 1 and 5, between 1 and 4, between 1 and 3, or between 1 and 2 compared to a corn plant or seed without the one or more, two or more, three or more, four or more, five or more, six or more, or seven or more NLB resistance QTLs or NLB resistance alleles under a high NLB stress condition.
[0148] In another aspect, a corn plant or seed provided herein comprising one or more, two or more, three or more, four or more, five or more, six or more, or seven or more NLB resistance QTLs selected from the group consisting of NLB resistance QTLs NLB_2.01, NLB_3.01, NLB_4.01, NLB_4.02, NLB_5.01, NLB_6.01, NLB_7.01, and NLB_9.01 exhibits a reduction of NLB rating score of between 5% and 90%, 5% and 80%, between 5% and 70%, between 5% and 60%, between 5% and 50%, between 5% and 40%, between 5% and 30%, between 5% and 20%, between 5% and 15%, or between 5% and 10%, compared to a corn plant or seed without the one or more, two or more, three or more, four or more, five or more, six or more, or seven or more NLB resistance QTLs under a high NLB stress condition.
[0149] In an aspect, a corn plant or seed provided herein comprising one or more, two or more, three or more, four or more, five or more, six or more, seven or more, eight or more, nine or more, ten or more, or eleven or more NLB resistance QTLs or NLB resistance alleles provided herein exhibits a seed yield increase of about 1% or more, 2% or more, 3% or more, 4% or more, 5% or more, 10% or more, 15% or more, 20% or more, 25% or more, 30% or more, 40% or more, 50% or more, 60% or more, 70% or more, 80% or more, 90% or more, or 100% or more than seed yield of a corn plant or seed without the one or more, two or more, three or more, four or more, five or more, six or more, seven or more, eight or more, nine or more, ten or more, or eleven or more NLB resistance QTLs or NLB resistance alleles under a high NLB stress condition.
[0150] In an aspect, a corn plant or seed provided herein as described in any of paragraphs [00175] to [00183] exhibits a seed yield increase of about 1% or more than seed yield of a corn plant or seed without the NLB resistance QTLs under a high NLB stress condition. In an aspect, a corn plant or seed provided herein comprising one or more, two or more, three or more, four or more, five or more, six or more, seven or more, eight or more, nine or more, ten or more, or eleven or more NLB resistance alleles provided herein exhibits a seed yield increase of about 1% or more than seed yield of a corn plant or seed without the one or more, two or more, three or more, four or more, five or more, six or more, seven or more, eight or more, nine or more, ten or more, or eleven or more NLB resistance alleles under a high NLB stress condition.
[0151] In an aspect, a corn plant or seed provided herein as described in any of paragraphs [00175] to [00183] exhibits a seed yield increase of about 2% or more than seed yield of a corn plant or seed without the NLB resistance QTLs under a high NLB stress condition. In an aspect, a corn plant or seed provided herein comprising one or more, two or more, three or more, four or more, five or more, six or more, seven or more, eight or more, nine or more, ten or more, or eleven or more NLB resistance alleles provided herein exhibits a seed yield increase of about 2% or more than seed yield of a corn plant or seed without the one or more, two or more, three or more, four or more, five or more, six or more, seven or more, eight or more, nine or more, ten or more, or eleven or more NLB resistance alleles under a high NLB stress condition.
[0152] In an aspect, a corn plant or seed provided herein as described in any of paragraphs [00175] to [00183] exhibits a seed yield increase of about 3% or more than seed yield of a corn plant or seed without the NLB resistance QTLs under a high NLB stress condition. In an aspect, a corn plant or seed provided herein comprising one or more, two or more, three or more, four or more, five or more, six or more, seven or more, eight or more, nine or more, ten or more, or eleven or more NLB resistance alleles provided herein exhibits a seed yield increase of about 3% or more than seed yield of a corn plant or seed without the one or more, two or more, three or more, four or more, five or more, six or more, seven or more, eight or more, nine or more, ten or more, or eleven or more NLB resistance alleles under a high NLB stress condition.
[0153] In an aspect, a corn plant or seed provided herein as described in any of paragraphs [00175] to [00183] exhibits a seed yield increase of about 4% or more than seed yield of a corn plant or seed without the NLB resistance QTLs under a high NLB stress condition. In an aspect, a corn plant or seed provided herein comprising one or more, two or more, three or more, four or more, five or more, six or more, seven or more, eight or more, nine or more, ten or more, or eleven or more NLB resistance alleles provided herein exhibits a seed yield increase of about 4% or more than seed yield of a corn plant or seed without the one or more, two or more, three or more, four or more, five or more, six or more, seven or more, eight or more, nine or more, ten or more, or eleven or more NLB resistance alleles under a high NLB stress condition.
[0154] In an aspect, a corn plant or seed provided herein as described in any of paragraphs [00175] to [00183] exhibits a seed yield increase of about 5% or more than seed yield of a corn plant or seed without the NLB resistance QTLs under a high NLB stress condition. In an aspect, a corn plant or seed provided herein comprising one or more, two or more, three or more, four or more, five or more, six or more, seven or more, eight or more, nine or more, ten or more, or eleven or more NLB resistance alleles provided herein exhibits a seed yield increase of about 5% or more than seed yield of a corn plant or seed without the one or more, two or more, three or more, four or more, five or more, six or more, seven or more, eight or more, nine or more, ten or more, or eleven or more NLB resistance alleles under a high NLB stress condition.
[0155] In an aspect, a corn plant or seed provided herein as described in any of paragraphs [00175] to [00183] exhibits a seed yield increase of about 10% or more than seed yield of a corn plant or seed without the NLB resistance QTLs under a high NLB stress condition. In an aspect, a corn plant or seed provided herein comprising one or more, two or more, three or more, four or more, five or more, six or more, seven or more, eight or more, nine or more, ten or more, or eleven or more NLB resistance alleles provided herein exhibits a seed yield increase of about 10% or more than seed yield of a corn plant or seed without the one or more, two or more, three or more, four or more, five or more, six or more, seven or more, eight or more, nine or more, ten or more, or eleven or more NLB resistance alleles under a high NLB stress condition.
[0156] In an aspect, a corn plant or seed provided herein as described in any of paragraphs [00175] to [00183] exhibits a seed yield increase of about 15% or more than seed yield of a corn plant or seed without the NLB resistance QTLs under a high NLB stress condition. In an aspect, a corn plant or seed provided herein comprising one or more, two or more, three or more, four or more, five or more, six or more, seven or more, eight or more, nine or more, ten or more, or eleven or more NLB resistance alleles provided herein exhibits a seed yield increase of about 15% or more than seed yield of a corn plant or seed without the one or more, two or more, three or more, four or more, five or more, six or more, seven or more, eight or more, nine or more, ten or more, or eleven or more NLB resistance alleles under a high NLB stress condition.
[0157] In an aspect, a corn plant or seed provided herein as described in any of paragraphs [00175] to [00183] exhibits a seed yield increase of about 20% or more than seed yield of a corn plant or seed without the NLB resistance QTLs under a high NLB stress condition. In an aspect, a corn plant or seed provided herein comprising one or more, two or more, three or more, four or more, five or more, six or more, seven or more, eight or more, nine or more, ten or more, or eleven or more NLB resistance alleles provided herein exhibits a seed yield increase of about 20% or more than seed yield of a corn plant or seed without the one or more, two or more, three or more, four or more, five or more, six or more, seven or more, eight or more, nine or more, ten or more, or eleven or more NLB resistance alleles under a high NLB stress condition.
[0158] In an aspect, a corn plant or seed provided herein as described in any of paragraphs [00175] to [00183] exhibits a seed yield increase of about 25% or more than seed yield of a corn plant or seed without the NLB resistance QTLs under a high NLB stress condition. In an aspect, a corn plant or seed provided herein comprising one or more, two or more, three or more, four or more, five or more, six or more, seven or more, eight or more, nine or more, ten or more, or eleven or more NLB resistance alleles provided herein exhibits a seed yield increase of about 25% or more than seed yield of a corn plant or seed without the one or more, two or more, three or more, four or more, five or more, six or more, seven or more, eight or more, nine or more, ten or more, or eleven or more NLB resistance alleles under a high NLB stress condition.
[0159] In an aspect, a corn plant or seed provided herein as described in any of paragraphs [00175] to [00183] exhibits a seed yield increase of about 30% or more than seed yield of a corn plant or seed without the NLB resistance QTLs under a high NLB stress condition. In an aspect, a corn plant or seed provided herein comprising one or more, two or more, three or more, four or more, five or more, six or more, seven or more, eight or more, nine or more, ten or more, or eleven or more NLB resistance alleles provided herein exhibits a seed yield increase of about 30% or more than seed yield of a corn plant or seed without the one or more, two or more, three or more, four or more, five or more, six or more, seven or more, eight or more, nine or more, ten or more, or eleven or more NLB resistance alleles under a high NLB stress condition.
[0160] In an aspect, a corn plant or seed provided herein as described in any of paragraphs [00175] to [00183] exhibits a seed yield increase of about 40% or more than seed yield of a corn plant or seed without the NLB resistance QTLs under a high NLB stress condition. In an aspect, a corn plant or seed provided herein comprising one or more, two or more, three or more, four or more, five or more, six or more, seven or more, eight or more, nine or more, ten or more, or eleven or more NLB resistance alleles provided herein exhibits a seed yield increase of about 40% or more than seed yield of a corn plant or seed without the one or more, two or more, three or more, four or more, five or more, six or more, seven or more, eight or more, nine or more, ten or more, or eleven or more NLB resistance alleles under a high NLB stress condition.
[0161] In an aspect, a corn plant or seed provided herein as described in any of paragraphs [00175] to [00183] exhibits a seed yield increase of about 50% or more than seed yield of a corn plant or seed without the NLB resistance QTLs under a high NLB stress condition. In an aspect, a corn plant or seed provided herein comprising one or more, two or more, three or more, four or more, five or more, six or more, seven or more, eight or more, nine or more, ten or more, or eleven or more NLB resistance alleles provided herein exhibits a seed yield increase of about 50% or more than seed yield of a corn plant or seed without the one or more, two or more, three or more, four or more, five or more, six or more, seven or more, eight or more, nine or more, ten or more, or eleven or more NLB resistance alleles under a high NLB stress condition.
[0162] In an aspect, a corn plant or seed provided herein as described in any of paragraphs [00175] to [00183] exhibits a seed yield increase of about 60% or more than seed yield of a corn plant or seed without the NLB resistance QTLs under a high NLB stress condition. In an aspect, a corn plant or seed provided herein comprising one or more, two or more, three or more, four or more, five or more, six or more, seven or more, eight or more, nine or more, ten or more, or eleven or more NLB resistance alleles provided herein exhibits a seed yield increase of about 60% or more than seed yield of a corn plant or seed without the one or more, two or more, three or more, four or more, five or more, six or more, seven or more, eight or more, nine or more, ten or more, or eleven or more NLB resistance alleles under a high NLB stress condition.
[0163] In an aspect, a corn plant or seed provided herein as described in any of paragraphs [00175] to [00183] exhibits a seed yield increase of about 70% or more than seed yield of a corn plant or seed without the NLB resistance QTLs under a high NLB stress condition. In an aspect, a corn plant or seed provided herein comprising one or more, two or more, three or more, four or more, five or more, six or more, seven or more, eight or more, nine or more, ten or more, or eleven or more NLB resistance alleles provided herein exhibits a seed yield increase of about 70% or more than seed yield of a corn plant or seed without the one or more, two or more, three or more, four or more, five or more, six or more, seven or more, eight or more, nine or more, ten or more, or eleven or more NLB resistance alleles under a high NLB stress condition.
[0164] In an aspect, a corn plant or seed provided herein as described in any of paragraphs [00175] to [00183] exhibits a seed yield increase of about 80% or more than seed yield of a corn plant or seed without the NLB resistance QTLs under a high NLB stress condition. In an aspect, a corn plant or seed provided herein comprising one or more, two or more, three or more, four or more, five or more, six or more, seven or more, eight or more, nine or more, ten or more, or eleven or more NLB resistance alleles provided herein exhibits a seed yield increase of about 80% or more than seed yield of a corn plant or seed without the one or more, two or more, three or more, four or more, five or more, six or more, seven or more, eight or more, nine or more, ten or more, or eleven or more NLB resistance alleles under a high NLB stress condition.
[0165] In an aspect, a corn plant or seed provided herein as described in any of paragraphs [00175] to [00183] exhibits a seed yield increase of about 90% or more than seed yield of a corn plant or seed without the NLB resistance QTLs under a high NLB stress condition. In an aspect, a corn plant or seed provided herein comprising one or more, two or more, three or more, four or more, five or more, six or more, seven or more, eight or more, nine or more, ten or more, or eleven or more NLB resistance alleles provided herein exhibits a seed yield increase of about 90% or more than seed yield of a corn plant or seed without the one or more, two or more, three or more, four or more, five or more, six or more, seven or more, eight or more, nine or more, ten or more, or eleven or more NLB resistance alleles under a high NLB stress condition.
[0166] In an aspect, a corn plant or seed provided herein as described in any of paragraphs [00175] to [00183] exhibits a seed yield increase of about 100% or more than seed yield of a corn plant or seed without the NLB resistance QTLs under a high NLB stress condition. In an aspect, a corn plant or seed provided herein comprising one or more, two or more, three or more, four or more, five or more, six or more, seven or more, eight or more, nine or more, ten or more, or eleven or more NLB resistance alleles provided herein exhibits a seed yield increase of about 100% or more than seed yield of a corn plant or seed without the one or more, two or more, three or more, four or more, five or more, six or more, seven or more, eight or more, nine or more, ten or more, or eleven or more NLB resistance alleles under a high NLB stress condition.
[0167] In an aspect, a corn plant or seed provided herein comprising one or more, two or more, three or more, four or more, five or more, six or more, or seven or more NLB resistance QTLs selected from the group consisting of NLB resistance QTLs NLB_2.01, NLB_3.01, NLB_4.01, NLB_4.02, NLB_5.01, NLB_6.01, NLB_7.01, and NLB_9.01 exhibits a seed yield increase of about 1% or more, 2% or more, 3% or more, 4% or more, 5% or more, 10% or more, 15% or more, 20% or more, 25% or more, 30% or more, 40% or more, 50% or more, 60% or more, 70% or more, 80% or more, 90% or more, or 100% or more than seed yield of a corn plant or seed without the one or more, two or more, three or more, four or more, five or more, six or more, or seven or more NLB resistance QTLs under a high NLB stress condition.
[0168] In another aspect, a corn plant or seed provided herein comprising one or more, two or more, three or more, four or more, five or more, six or more, or seven or more NLB resistance QTLs or NLB resistance alleles provided herein exhibits a seed yield increase of between 1% and 100%, between 1% and 90%, between 1% and 80%, between 1% and 70%, between 1% and 60%, between 1% and 50%, between 1% and 40%, between 1% and 30%, between 1% and 25%, between 1% and 20%, between 1% and 15%, between 1% and 10%, between 1% and 5%, between 1% and 4%, between 1% and 3%, between 1% and 2%, between 2% and 90%, between 3% and 80%, between 4% and 70%, between 5% and 60%, between 10% and 50%, between 15% and 40%, between 20% and 30%, or between 5% and 25% of seed yield of a corn plant or seed without the one or more, two or more, three or more, four or more, five or more, six or more, or seven or more NLB resistance QTLs or NLB resistance alleles under a high NLB stress condition.
[0169] In an aspect, a corn plant or seed provided herein comprising one or more, two or more, three or more, four or more, five or more, six or more, or seven or more NLB resistance QTLs selected from the group consisting of NLB resistance QTLs NLB_2.01, NLB_3.01, NLB_4.01, NLB_4.02, NLB_5.01, NLB_6.01, NLB_7.01, and NLB_9.01 exhibits a seed yield increase of between 1% and 100%, between 1% and 90%, between 1% and 80%, between 1% and 70%, between 1% and 60%, between 1% and 50%, between 1% and 40%, between 1% and 30%, between 1% and 25%, between 1% and 20%, between 1% and 15%, between 1% and 10%, between 1% and 5%, between 1% and 4%, between 1% and 3%, between 1% and 2%, between 2% and 90%, between 3% and 80%, between 4% and 70%, between 5% and 60%, between 10% and 50%, between 15% and 40%, between 20% and 30%, or between 5% and 25% of seed yield of a corn plant or seed without the one or more, two or more, three or more, four or more, five or more, six or more, or seven or more NLB resistance QTLs under a high NLB stress condition. In an aspect, a corn plant or seed provided herein as described in any of paragraphs [00175] to [00183] exhibits a seed yield increase of between 1% and 100% compared to the seed yield of a corn plant or seed without the NLB resistance QTLs under a high NLB stress condition. In an aspect, a corn plant or seed provided herein as described in any of paragraphs [00175] to [00183] exhibits a seed yield increase of between 1% and 90% compared to the seed yield of a corn plant or seed without the NLB resistance QTLs under a high NLB stress condition. In an aspect, a corn plant or seed provided herein as described in any of paragraphs [00175] to [00183] exhibits a seed yield increase of between 1% and 80% compared to the seed yield of a corn plant or seed without the NLB resistance QTLs under a high NLB stress condition. In an aspect, a corn plant or seed provided herein as described in any of paragraphs [00175] to [00183] exhibits a seed yield increase of between 1% and 70% compared to the seed yield of a corn plant or seed without the NLB resistance QTLs under a high NLB stress condition. In an aspect, a corn plant or seed provided herein as described in any of paragraphs [00175] to [00183] exhibits a seed yield increase of between 1% and 60% compared to the seed yield of a corn plant or seed without the NLB resistance QTLs under a high NLB stress condition. In an aspect, a corn plant or seed provided herein as described in any of paragraphs [00175] to [00183] exhibits a seed yield increase of between 1% and 50% compared to the seed yield of a corn plant or seed without the NLB resistance QTLs under a high NLB stress condition. In an aspect, a corn plant or seed provided herein as described in any of paragraphs [00175] to [00183] exhibits a seed yield increase of between 1% and 40% compared to the seed yield of a corn plant or seed without the NLB resistance QTLs under a high NLB stress condition. In an aspect, a corn plant or seed provided herein as described in any of paragraphs [00175] to [00183] exhibits a seed yield increase of between 1% and 30% compared to the seed yield of a corn plant or seed without the NLB resistance QTLs under a high NLB stress condition. In an aspect, a corn plant or seed provided herein as described in any of paragraphs [00175] to [00183] exhibits a seed yield increase of between 1% and 25% compared to the seed yield of a corn plant or seed without the NLB resistance QTLs under a high NLB stress condition. In an aspect, a corn plant or seed provided herein as described in any of paragraphs [00175] to [00183] exhibits a seed yield increase of between 1% and 20% compared to the seed yield of a corn plant or seed without the NLB resistance QTLs under a high NLB stress condition. In an aspect, a corn plant or seed provided herein as described in any of paragraphs [00175] to [00183] exhibits a seed yield increase of between 1% and 15% compared to the seed yield of a corn plant or seed without the NLB resistance QTLs under a high NLB stress condition. In an aspect, a corn plant or seed provided herein as described in any of paragraphs [00175] to [00183] exhibits a seed yield increase of between 1% and 10% compared to the seed yield of a corn plant or seed without the NLB resistance QTLs under a high NLB stress condition. In an aspect, a corn plant or seed provided herein as described in any of paragraphs [00175] to [00183] exhibits a seed yield increase of between 1% and 5% compared to the seed yield of a corn plant or seed without the NLB resistance QTLs under a high NLB stress condition. In an aspect, a corn plant or seed provided herein as described in any of paragraphs [00175] to [00183] exhibits a seed yield increase of between 1% and 4% compared to the seed yield of a corn plant or seed without the NLB resistance QTLs under a high NLB stress condition. In an aspect, a corn plant or seed provided herein as described in any of paragraphs [00175] to [00183] exhibits a seed yield increase of between 1% and 3% compared to the seed yield of a corn plant or seed without the NLB resistance QTLs under a high NLB stress condition. In an aspect, a corn plant or seed provided herein as described in any of paragraphs [00175] to [00183] exhibits a seed yield increase of between 1% and 2% compared to the seed yield of a corn plant or seed without the NLB resistance QTLs under a high NLB stress condition. In an aspect, a corn plant or seed provided herein as described in any of paragraphs [00175] to [00183] exhibits a seed yield increase of between 2% and 90% compared to the seed yield of a corn plant or seed without the NLB resistance QTLs under a high NLB stress condition. In an aspect, a corn plant or seed provided herein as described in any of paragraphs [00175] to [00183] exhibits a seed yield increase of between 3% and 80% compared to the seed yield of a corn plant or seed without the NLB resistance QTLs under a high NLB stress condition. In an aspect, a corn plant or seed provided herein as described in any of paragraphs [00175] to [00183] exhibits a seed yield increase of between 4% and 70% compared to the seed yield of a corn plant or seed without the NLB resistance QTLs under a high NLB stress condition. In an aspect, a corn plant or seed provided herein as described in any of paragraphs [00175] to [00183] exhibits a seed yield increase of between 5% and 60% compared to the seed yield of a corn plant or seed without the NLB resistance QTLs under a high NLB stress condition. In an aspect, a corn plant or seed provided herein as described in any of paragraphs [00175] to [00183] exhibits a seed yield increase of between 10% and 50% compared to the seed yield of a corn plant or seed without the NLB resistance QTLs under a high NLB stress condition. In an aspect, a corn plant or seed provided herein as described in any of paragraphs [00175] to [00183] exhibits a seed yield increase of between 15% and 40% compared to the seed yield of a corn plant or seed without the NLB resistance QTLs under a high NLB stress condition. In an aspect, a corn plant or seed provided herein as described in any of paragraphs [00175] to [00183] exhibits a seed yield increase of between 20% and 30% compared to the seed yield of a corn plant or seed without the NLB resistance QTLs under a high NLB stress condition. In an aspect, a corn plant or seed provided herein as described in any of paragraphs [00175] to [00183] exhibits a seed yield increase of between 5% and 25% compared to the seed yield of a corn plant or seed without the NLB resistance QTLs under a high NLB stress condition.
[0170] In an aspect, a corn plant or seed provided herein comprising one or more, two or more, three or more, four or more, five or more, six or more, or seven or more NLB resistance QTLs or NLB resistance alleles provided herein exhibits a seed yield about 0.1 quintal/hectare or more, 0.25 quintal/hectare or more, 0.5 quintal/hectare or more, 0.75 quintal/hectare or more, 1 quintal/hectare or more, 1.5 quintal/hectare or more, 2 quintal/hectare or more, 2.5 quintal/hectare or more, 3 quintal/hectare or more, 3.5 quintal/hectare or more, 4 quintal/hectare or more, 4.5 quintal/hectare or more, 5 quintal/hectare or more, 6 quintal/hectare or more, 7 quintal/hectare or more, 8 quintal/hectare or more, 9 quintal/hectare or more, or 10 quintal/hectare or more higher than seed yield of a corn plant or seed without the one or more, two or more, three or more, four or more, five or more, six or more, or seven or more NLB resistance QTLs or NLB resistance alleles under a high NLB stress condition.
[0171] In an aspect, a corn plant or seed provided herein comprising one or more, two or more, three or more, four or more, five or more, six or more, or seven or more NLB resistance QTLs selected from the group consisting of NLB resistance QTLs NLB_2.01, NLB_3.01, NLB_4.01, NLB_4.02, NLB_5.01, NLB_6.01, NLB_7.01, and NLB_9.01 exhibits a seed yield about 0.1 quintal/hectare or more, 0.25 quintal/hectare or more, 0.5 quintal/hectare or more, 0.75 quintal/hectare or more, 1 quintal/hectare or more, 1.5 quintal/hectare or more, 2 quintal/hectare or more, 2.5 quintal/hectare or more, 3 quintal/hectare or more, 3.5 quintal/hectare or more, 4 quintal/hectare or more, 4.5 quintal/hectare or more, 5 quintal/hectare or more, 6 quintal/hectare or more, 7 quintal/hectare or more, 8 quintal/hectare or more, 9 quintal/hectare or more, or 10 quintal/hectare or more higher than seed yield of a corn plant or seed without the one or more, two or more, three or more, four or more, five or more, six or more, or seven or more NLB resistance QTLs under a high NLB stress condition. In an aspect, a corn plant or seed provided herein as described in any of paragraphs [00175] to [00183] exhibits a seed yield increase of about 0.1 quintal/hectare or more than seed yield of a corn plant or seed without the NLB resistance QTLs under a high NLB stress condition. In an aspect, a corn plant or seed provided herein as described in any of paragraphs [00175] to [00183] exhibits a seed yield increase of about 0.25 quintal/hectare or more than seed yield of a corn plant or seed without the NLB resistance QTLs under a high NLB stress condition. In an aspect, a corn plant or seed provided herein as described in any of paragraphs [00175] to [00183] exhibits a seed yield increase of about 0.5 quintal/hectare or more than seed yield of a corn plant or seed without the NLB resistance QTLs under a high NLB stress condition. In an aspect, a corn plant or seed provided herein as described in any of paragraphs [00175] to [00183] exhibits a seed yield increase of about 0.75 quintal/hectare or more than seed yield of a corn plant or seed without the NLB resistance QTLs under a high NLB stress condition. In an aspect, a corn plant or seed provided herein as described in any of paragraphs [00175] to [00183] exhibits a seed yield increase of about 1 quintal/hectare or more than seed yield of a corn plant or seed without the NLB resistance QTLs under a high NLB stress condition. In an aspect, a corn plant or seed provided herein as described in any of paragraphs [00175] to [00183] exhibits a seed yield increase of about 1.5 quintal/hectare or more than seed yield of a corn plant or seed without the NLB resistance QTLs under a high NLB stress condition. In an aspect, a corn plant or seed provided herein as described in any of paragraphs [00175] to [00183] exhibits a seed yield increase of about 2 quintal/hectare or more than seed yield of a corn plant or seed without the NLB resistance QTLs under a high NLB stress condition. In an aspect, a corn plant or seed provided herein as described in any of paragraphs [00175] to [00183] exhibits a seed yield increase of about 2.5 quintal/hectare or more than seed yield of a corn plant or seed without the NLB resistance QTLs under a high NLB stress condition. In an aspect, a corn plant or seed provided herein as described in any of paragraphs [00175] to [00183] exhibits a seed yield increase of about 3 quintal/hectare or more than seed yield of a corn plant or seed without the NLB resistance QTLs under a high NLB stress condition. In an aspect, a corn plant or seed provided herein as described in any of paragraphs [00175] to [00183] exhibits a seed yield increase of about 0.1 quintal/hectare or more than seed yield of a corn plant or seed without the NLB resistance QTLs under a high NLB stress condition. In an aspect, a corn plant or seed provided herein as described in any of paragraphs [00175] to [00183] exhibits a seed yield increase of about 4 quintal/hectare or more than seed yield of a corn plant or seed without the NLB resistance QTLs under a high NLB stress condition. In an aspect, a corn plant or seed provided herein as described in any of paragraphs [00175] to [00183] exhibits a seed yield increase of about 4.5 quintal/hectare or more than seed yield of a corn plant or seed without the NLB resistance QTLs under a high NLB stress condition. In an aspect, a corn plant or seed provided herein as described in any of paragraphs [00175] to [00183] exhibits a seed yield increase of about 5 quintal/hectare or more than seed yield of a corn plant or seed without the NLB resistance QTLs under a high NLB stress condition. In an aspect, a corn plant or seed provided herein as described in any of paragraphs [00175] to [00183] exhibits a seed yield increase of about 6 quintal/hectare or more than seed yield of a corn plant or seed without the NLB resistance QTLs under a high NLB stress condition. In an aspect, a corn plant or seed provided herein as described in any of paragraphs [00175] to [00183] exhibits a seed yield increase of about 7 quintal/hectare or more than seed yield of a corn plant or seed without the NLB resistance QTLs under a high NLB stress condition. In an aspect, a corn plant or seed provided herein as described in any of paragraphs [00175] to [00183] exhibits a seed yield increase of about 8 quintal/hectare or more than seed yield of a corn plant or seed without the NLB resistance QTLs under a high NLB stress condition. In an aspect, a corn plant or seed provided herein as described in any of paragraphs [00175] to [00183] exhibits a seed yield increase of about 9 quintal/hectare or more than seed yield of a corn plant or seed without the NLB resistance QTLs under a high NLB stress condition. In an aspect, a corn plant or seed provided herein as described in any of paragraphs [00175] to [00183] exhibits a seed yield increase of about 10 quintal/hectare or more than seed yield of a corn plant or seed without the NLB resistance QTLs under a high NLB stress condition.
[0172] In another aspect, a corn plant or seed provided herein comprising one or more, two or more, three or more, four or more, five or more, six or more, or seven or more NLB resistance QTLs or NLB resistance alleles provided herein exhibits a seed yield between 0.1 and 10 quintal/hectare, between 0.1 and 9 quintal/hectare, between 0.1 and 8 quintal/hectare, between 0.1 and 7 quintal/hectare, between 0.1 and 6 quintal/hectare, between 0.1 and 5 quintal/hectare, between 0.1 and 4.5 quintal/hectare, between 0.1 and 4 quintal/hectare, between 0.1 and 3.5 quintal/hectare, between 0.1 and 3 quintal/hectare, between 0.1 and 2.5 quintal/hectare, between 0.1 and 2 quintal/hectare, between 0.1 and 1.5 quintal/hectare, between 0.1 and 1 quintal/hectare, between 0.1 and 0.75 quintal/hectare, between 0.1 and 0.5 quintal/hectare, between 0.1 and 0.25 quintal/hectare, between 0.25 and 9 quintal/hectare, between 0.5 and 8 quintal/hectare, between 0.75 and 7 quintal/hectare, between 1 and 6 quintal/hectare, between 1.5 and 5 quintal/hectare, between 2 and 4.5 quintal/hectare, between 2.5 and 4 quintal/hectare, or between 3 and 3.5 quintal/hectare higher than seed yield of a corn plant or seed without the one or more, two or more, three or more, four or more, five or more, six or more, or seven or more NLB resistance QTLs or NLB resistance alleles under a high NLB stress condition. In an aspect, a corn plant or seed provided herein as described in any of paragraphs [00175] to [00183] exhibits a seed yield between 0.1 and 10 quintal/hectare higher than seed yield of a corn plant or seed without the NLB resistance QTLs under a high NLB stress condition. In an aspect, a corn plant or seed provided herein as described in any of paragraphs [00175] to [00183] exhibits a seed yield between 0.1 and 9 quintal/hectare higher than seed yield of a corn plant or seed without the NLB resistance QTLs under a high NLB stress condition. In an aspect, a corn plant or seed provided herein as described in any of paragraphs [00175] to [00183] exhibits a seed yield between 0.1 and 8 quintal/hectare higher than seed yield of a corn plant or seed without the NLB resistance QTLs under a high NLB stress condition. In an aspect, a corn plant or seed provided herein as described in any of paragraphs [00175] to [00183] exhibits a seed yield between 0.1 and 7 quintal/hectare higher than seed yield of a corn plant or seed without the NLB resistance QTLs under a high NLB stress condition. In an aspect, a corn plant or seed provided herein as described in any of paragraphs [00175] to [00183] exhibits a seed yield between 0.1 and 6 quintal/hectare higher than seed yield of a corn plant or seed without the NLB resistance QTLs under a high NLB stress condition. In an aspect, a corn plant or seed provided herein as described in any of paragraphs [00175] to [00183] exhibits a seed yield between 0.1 and 5 quintal/hectare higher than seed yield of a corn plant or seed without the NLB resistance QTLs under a high NLB stress condition. In an aspect, a corn plant or seed provided herein as described in any of paragraphs [00175] to [00183] exhibits a seed yield between 0.1 and 4.5 quintal/hectare higher than seed yield of a corn plant or seed without the NLB resistance QTLs under a high NLB stress condition. In an aspect, a corn plant or seed provided herein as described in any of paragraphs [00175] to [00183] exhibits a seed yield between 0.1 and 4 quintal/hectare higher than seed yield of a corn plant or seed without the NLB resistance QTLs under a high NLB stress condition. In an aspect, a corn plant or seed provided herein as described in any of paragraphs [00175] to [00183] exhibits a seed yield between 0.1 and 3.5 quintal/hectare higher than seed yield of a corn plant or seed without the NLB resistance QTLs under a high NLB stress condition. In an aspect, a corn plant or seed provided herein as described in any of paragraphs [00175] to [00183] exhibits a seed yield between 0.1 and 3 quintal/hectare higher than seed yield of a corn plant or seed without the NLB resistance QTLs under a high NLB stress condition. In an aspect, a corn plant or seed provided herein as described in any of paragraphs [00175] to [00183] exhibits a seed yield between 0.1 and 2.5 quintal/hectare higher than seed yield of a corn plant or seed without the NLB resistance QTLs under a high NLB stress condition. In an aspect, a corn plant or seed provided herein as described in any of paragraphs [00175] to [00183] exhibits a seed yield between 0.1 and 2 quintal/hectare higher than seed yield of a corn plant or seed without the NLB resistance QTLs under a high NLB stress condition. In an aspect, a corn plant or seed provided herein as described in any of paragraphs [00175] to [00183] exhibits a seed yield between 0.1 and 1.5 quintal/hectare higher than seed yield of a corn plant or seed without the NLB resistance QTLs under a high NLB stress condition. In an aspect, a corn plant or seed provided herein as described in any of paragraphs [00175] to [00183] exhibits a seed yield between 0.1 and 1 quintal/hectare higher than seed yield of a corn plant or seed without the NLB resistance QTLs under a high NLB stress condition. In an aspect, a corn plant or seed provided herein as described in any of paragraphs [00175] to [00183] exhibits a seed yield between 0.1 and 0.75 quintal/hectare higher than seed yield of a corn plant or seed without the NLB resistance QTLs under a high NLB stress condition. In an aspect, a corn plant or seed provided herein as described in any of paragraphs [00175] to [00183] exhibits a seed yield between 0.1 and 0.5 quintal/hectare higher than seed yield of a corn plant or seed without the NLB resistance QTLs under a high NLB stress condition. In an aspect, a corn plant or seed provided herein as described in any of paragraphs [00175] to [00183] exhibits a seed yield between 0.1 and 0.25 quintal/hectare higher than seed yield of a corn plant or seed without the NLB resistance QTLs under a high NLB stress condition. In an aspect, a corn plant or seed provided herein as described in any of paragraphs [00175] to [00183] exhibits a seed yield between 0.25 and 9 quintal/hectare higher than seed yield of a corn plant or seed without the NLB resistance QTLs under a high NLB stress condition. In an aspect, a corn plant or seed provided herein as described in any of paragraphs [00175] to [00183] exhibits a seed yield between 0.5 and 8 quintal/hectare higher than seed yield of a corn plant or seed without the NLB resistance QTLs under a high NLB stress condition. In an aspect, a corn plant or seed provided herein as described in any of paragraphs [00175] to [00183] exhibits a seed yield between 0.75 and 7 quintal/hectare higher than seed yield of a corn plant or seed without the NLB resistance QTLs under a high NLB stress condition. In an aspect, a corn plant or seed provided herein as described in any of paragraphs [00175] to [00183] exhibits a seed yield between 1 and 6 quintal/hectare higher than seed yield of a corn plant or seed without the NLB resistance QTLs under a high NLB stress condition. In an aspect, a corn plant or seed provided herein as described in any of paragraphs [00175] to [00183] exhibits a seed yield between 1.5 and 5 quintal/hectare higher than seed yield of a corn plant or seed without the NLB resistance QTLs under a high NLB stress condition. In an aspect, a corn plant or seed provided herein as described in any of paragraphs [00175] to [00183] exhibits a seed yield between 2 and 4 quintal/hectare higher than seed yield of a corn plant or seed without the NLB resistance QTLs under a high NLB stress condition. In an aspect, a corn plant or seed provided herein as described in any of paragraphs [00175] to [00183] exhibits a seed yield between 3 and 3.5 quintal/hectare higher than seed yield of a corn plant or seed without the NLB resistance QTLs under a high NLB stress condition.
[0173] In an aspect, a corn plant or seed provided herein comprising one or more, two or more, three or more, four or more, five or more, six or more, or seven or more NLB resistance QTLs selected from the group consisting of NLB resistance QTLs NLB_2.01, NLB_3.01, NLB_4.01, NLB_4.02, NLB_5.01, NLB_7.01, NLB_6.01, and NLB_9.01 exhibits a seed yield between 0.1 and 10 quintal/hectare, between 0.1 and 9 quintal/hectare, between 0.1 and 8 quintal/hectare, between 0.1 and 7 quintal/hectare, between 0.1 and 6 quintal/hectare, between 0.1 and 5 quintal/hectare, between 0.1 and 4.5 quintal/hectare, between 0.1 and 4 quintal/hectare, between 0.1 and 3.5 quintal/hectare, between 0.1 and 3 quintal/hectare, between 0.1 and 2.5 quintal/hectare, between 0.1 and 2 quintal/hectare, between 0.1 and 1.5 quintal/hectare, between 0.1 and 1 quintal/hectare, between 0.1 and 0.75 quintal/hectare, between 0.1 and 0.5 quintal/hectare, between 0.1 and 0.25 quintal/hectare, between 0.25 and 9 quintal/hectare, between 0.5 and 8 quintal/hectare, between 0.75 and 7 quintal/hectare, between 1 and 6 quintal/hectare, between 1.5 and 5 quintal/hectare, between 2 and 4.5 quintal/hectare, between 2.5 and 4 quintal/hectare, or between 3 and 3.5 quintal/hectare higher than seed yield of a corn plant or seed without the one or more, two or more, three or more, four or more, five or more, six or more, or seven or more NLB resistance QTLs under a high NLB stress condition.
[0174] In an aspect, this disclosure provides an NLB resistant corn plant or seed comprising one or more, two or more, three or more, four or more, five or more, six or more, or seven or more introgressed NLB resistance QTLs selected from the group consisting of NLB resistance QTLs NLB_2.01, NLB_3.01, NLB_4.01, NLB_4.02, NLB_5.01, NLB_6.01, NLB_7.01, and NLB_9.01. In an aspect, a corn plant or seed provided herein comprises NLB resistance QTLs NLB_2.01, NLB_3.01, NLB_4.01, NLB_4.02, NLB_5.01, NLB_6.01, NLB_7.01, and NLB_9.01 obtainable, obtained, or introgressed from any one of corn lines CV114258, CV115214, CV099829, CV102084, CV095508, CV103141, CV105893, CV595358, CV593417, CV117407, CV592505, and CV592420.
[0175] In an aspect, a corn plant, seed, or cell provided herein comprises NLB resistance QTLs NLB_4.01, and NLB_4.02. In another aspect, a corn plant, seed, or cell provided herein comprises NLB resistance QTLs NLB_2.01, NLB_4.01, and NLB_4.02. In an aspect, a corn plant, seed, or cell provided herein comprises NLB resistance QTLs NLB_2.01 and NLB_4.01. In an aspect, a corn plant, seed, or cell provided herein comprises NLB resistance QTLs NLB_2.01 and NLB_4.02.
[0176] In another aspect, a corn plant, seed, or cell provided herein comprises NLB resistance QTL NLB_2.01 and one or more NLB resistance QTLs selected from the group consisting of NLB resistance QTLs NLB_3.01, NLB_4.01, NLB_4.02, NLB_5.01, NLB_6.01, NLB_7.01, and NLB_9.01. In another aspect, a corn plant, seed, or cell provided herein comprises NLB resistance QTL NLB_2.01 and two or more NLB resistance QTLs selected from the group consisting of NLB resistance QTLs NLB_3.01, NLB_4.01, NLB_4.02, NLB_5.01, NLB_6.01, NLB_7.01, and NLB_9.01. In another aspect, a corn plant, seed, or cell provided herein comprises NLB resistance QTL NLB_2.01 and three or more NLB resistance QTLs selected from the group consisting of NLB resistance QTLs NLB_3.01, NLB_4.01, NLB_4.02, NLB_5.01, NLB_6.01, NLB_7.01, and NLB_9.01. In another aspect, a corn plant, seed, or cell provided herein comprises NLB resistance QTL NLB_2.01 and four or more NLB resistance QTLs selected from the group consisting of NLB resistance QTLs NLB_3.01, NLB_4.01, NLB_4.02, NLB_5.01, NLB_6.01, NLB_7.01, and NLB_9.01. In another aspect, a corn plant, seed, or cell provided herein comprises NLB resistance QTL NLB_2.01 and five or more NLB resistance QTLs selected from the group consisting of NLB resistance QTLs NLB_3.01, NLB_4.01, NLB_4.02, NLB_5.01, NLB_6.01, NLB_7.01, and NLB_9.01. In another aspect, a corn plant, seed, or cell provided herein comprises NLB resistance QTL NLB_2.01 and or six or more NLB resistance QTLs selected from the group consisting of NLB resistance QTLs NLB_3.01, NLB_4.01, NLB_4.02, NLB_5.01, NLB_6.01, NLB_7.01, and NLB_9.01. In another aspect, a corn plant, seed, or cell provided herein comprises NLB resistance QTL NLB_2.01 and NLB resistance QTLs NLB_3.01, NLB_4.01, NLB_4.02, NLB_5.01, NLB_6.01, NLB_7.01, and NLB_9.01.
[0177] In another aspect, a corn plant, seed, or cell provided herein comprises NLB resistance QTL NLB_3.01 and one or more NLB resistance QTLs selected from the group consisting of NLB resistance QTLs NLB_2.01, NLB_4.01, NLB_4.02, NLB_5.01, NLB_6.01, NLB_7.01, and NLB_9.01. In another aspect, a corn plant, seed, or cell provided herein comprises NLB resistance QTL NLB_3.01 and two or more NLB resistance QTLs selected from the group consisting of NLB resistance QTLs NLB_2.01, NLB_4.01, NLB_4.02, NLB_5.01, NLB_6.01, NLB_7.01, and NLB_9.01. In another aspect, a corn plant, seed, or cell provided herein comprises NLB resistance QTL NLB_3.01 and three or more NLB resistance QTLs selected from the group consisting of NLB resistance QTLs NLB_2.01, NLB_4.01, NLB_4.02, NLB_5.01, NLB_6.01, NLB_7.01, and NLB_9.01. In another aspect, a corn plant, seed, or cell provided herein comprises NLB resistance QTL NLB_3.01 and four or more NLB resistance QTLs selected from the group consisting of NLB resistance QTLs NLB_2.01, NLB_4.01, NLB_4.02, NLB_5.01, NLB_6.01, NLB_7.01, and NLB_9.01. In another aspect, a corn plant, seed, or cell provided herein comprises NLB resistance QTL NLB_3.01 and five or more NLB resistance QTLs selected from the group consisting of NLB resistance QTLs NLB_2.01, NLB_4.01, NLB_4.02, NLB_5.01, NLB_6.01, NLB_7.01, and NLB_9.01. In another aspect, a corn plant, seed, or cell provided herein comprises NLB resistance QTL NLB_3.01 and or six or more NLB resistance QTLs selected from the group consisting of NLB resistance QTLs NLB_2.01, NLB_4.01, NLB_4.02, NLB_5.01, NLB_6.01, NLB_7.01, and NLB_9.01. In another aspect, a corn plant, seed, or cell provided herein comprises NLB resistance QTL NLB_3.01 and NLB resistance QTLs NLB_2.01, NLB_4.01, NLB_4.02, NLB_5.01, NLB_6.01, NLB_7.01, and NLB_9.01.
[0178] In another aspect, a corn plant, seed, or cell provided herein comprises NLB resistance QTL NLB_4.01 and one or more NLB resistance QTLs selected from the group consisting of NLB resistance QTLs NLB_2.01, NLB_3.01, NLB_4.02, NLB_5.01, NLB_6.01, NLB_7.01, and NLB_9.01. In another aspect, a corn plant, seed, or cell provided herein comprises NLB resistance QTL NLB_4.01 and two or more NLB resistance QTLs selected from the group consisting of NLB resistance QTLs NLB_2.01, NLB_3.01, NLB_4.02, NLB_5.01, NLB_6.01, NLB_7.01, and NLB_9.01. In another aspect, a corn plant, seed, or cell provided herein comprises NLB resistance QTL NLB_4.01 and three or more NLB resistance QTLs selected from the group consisting of NLB resistance QTLs NLB_2.01, NLB_3.01, NLB_4.02, NLB_5.01, NLB_6.01, NLB_7.01, and NLB_9.01. In another aspect, a corn plant, seed, or cell provided herein comprises NLB resistance QTL NLB_4.01 and four or more NLB resistance QTLs selected from the group consisting of NLB resistance QTLs NLB_2.01, NLB_3.01, NLB_4.02, NLB_5.01, NLB_6.01, NLB_7.01, and NLB_9.01. In another aspect, a corn plant, seed, or cell provided herein comprises NLB resistance QTL NLB_4.01 and five or more NLB resistance QTLs selected from the group consisting of NLB resistance QTLs NLB_2.01, NLB_3.01, NLB_4.02, NLB_5.01, NLB_6.01, NLB_7.01, and NLB_9.01. In another aspect, a corn plant, seed, or cell provided herein comprises NLB resistance QTL NLB_4.01 and or six or more NLB resistance QTLs selected from the group consisting of NLB resistance QTLs NLB_2.01, NLB_3.01, NLB_4.02, NLB_5.01, NLB_6.01, NLB_7.01, and NLB_9.01. In another aspect, a corn plant, seed, or cell provided herein comprises NLB resistance QTL NLB_4.01 and NLB resistance QTLs NLB_2.01, NLB_3.01, NLB_4.02, NLB_5.01, NLB_6.01, NLB_7.01, and NLB_9.01.
[0179] In another aspect, a corn plant, seed, or cell provided herein comprises NLB resistance QTL NLB_4.02 and one or more NLB resistance QTLs selected from the group consisting of NLB resistance QTLs NLB_2.01, NLB_3.01, NLB_4.01, NLB_5.01, NLB_6.01, NLB_7.01, and NLB_9.01. In another aspect, a corn plant, seed, or cell provided herein comprises NLB resistance QTL NLB_4.02 and two or more NLB resistance QTLs selected from the group consisting of NLB resistance QTLs NLB_2.01, NLB_3.01, NLB_4.01, NLB_5.01, NLB_6.01, NLB_7.01, and NLB_9.01. In another aspect, a corn plant, seed, or cell provided herein comprises NLB resistance QTL NLB_4.02 and three or more NLB resistance QTLs selected from the group consisting of NLB resistance QTLs NLB_2.01, NLB_3.01, NLB_4.01, NLB_5.01, NLB_6.01, NLB_7.01, and NLB_9.01. In another aspect, a corn plant, seed, or cell provided herein comprises NLB resistance QTL NLB_4.02 and four or more NLB resistance QTLs selected from the group consisting of NLB resistance QTLs NLB_2.01, NLB_3.01, NLB_4.01, NLB_5.01, NLB_6.01, NLB_7.01, and NLB_9.01. In another aspect, a corn plant, seed, or cell provided herein comprises NLB resistance QTL NLB_4.02 and five or more NLB resistance QTLs selected from the group consisting of NLB resistance QTLs NLB_2.01, NLB_3.01, NLB_4.01, NLB_5.01, NLB_6.01, NLB_7.01, and NLB_9.01. In another aspect, a corn plant, seed, or cell provided herein comprises NLB resistance QTL NLB_4.02 and or six or more NLB resistance QTLs selected from the group consisting of NLB resistance QTLs NLB_2.01, NLB_3.01, NLB_4.01, NLB_5.01, NLB_6.01, NLB_7.01, and NLB_9.01. In another aspect, a corn plant, seed, or cell provided herein comprises NLB resistance QTL NLB_4.02 and NLB resistance QTLs NLB_2.01, NLB_3.01, NLB_4.01, NLB_5.01, NLB_6.01, NLB_7.01, and NLB_9.01.
[0180] In another aspect, a corn plant, seed, or cell provided herein comprises NLB resistance QTL NLB_5.01 and one or more NLB resistance QTLs selected from the group consisting of NLB resistance QTLs NLB_2.01, NLB_3.01, NLB_4.01, NLB_4.02, NLB_6.01, NLB_7.01, and NLB_9.01. In another aspect, a corn plant, seed, or cell provided herein comprises NLB resistance QTL NLB_5.01 and two or more NLB resistance QTLs selected from the group consisting of NLB resistance QTLs NLB_2.01, NLB_3.01, NLB_4.01, NLB_4.02, NLB_6.01, NLB_7.01, and NLB_9.01. In another aspect, a corn plant, seed, or cell provided herein comprises NLB resistance QTL NLB_5.01 and three or more NLB resistance QTLs selected from the group consisting of NLB resistance QTLs NLB_2.01, NLB_3.01, NLB_4.01, NLB_4.02, NLB_6.01, NLB_7.01, and NLB_9.01. In another aspect, a corn plant, seed, or cell provided herein comprises NLB resistance QTL NLB_5.01 and four or more NLB resistance QTLs selected from the group consisting of NLB resistance QTLs NLB_2.01, NLB_3.01, NLB_4.01, NLB_4.02, NLB_6.01, NLB_7.01, and NLB_9.01. In another aspect, a corn plant, seed, or cell provided herein comprises NLB resistance QTL NLB_5.01 and five or more NLB resistance QTLs selected from the group consisting of NLB resistance QTLs NLB_2.01, NLB_3.01, NLB_4.01, NLB_4.02, NLB_6.01, NLB_7.01, and NLB_9.01. In another aspect, a corn plant, seed, or cell provided herein comprises NLB resistance QTL NLB_5.01 and or six or more NLB resistance QTLs selected from the group consisting of NLB resistance QTLs NLB_2.01, NLB_3.01, NLB_4.01, NLB_4.02, NLB_6.01, NLB_7.01, and NLB_9.01. In another aspect, a corn plant, seed, or cell provided herein comprises NLB resistance QTL NLB_5.01 and NLB resistance QTLs NLB_2.01, NLB_3.01, NLB_4.01, NLB_4.02, NLB_6.01, NLB_7.01, and NLB_9.01.
[0181] In another aspect, a corn plant, seed, or cell provided herein comprises NLB resistance QTL NLB_6.01 and one or more NLB resistance QTLs selected from the group consisting of NLB resistance QTLs NLB_2.01, NLB_3.01, NLB_4.01, NLB_4.02, NLB_5.01, NLB_7.01, and NLB_9.01. In another aspect, a corn plant, seed, or cell provided herein comprises NLB resistance QTL NLB_6.01 and two or more NLB resistance QTLs selected from the group consisting of NLB resistance QTLs NLB_2.01, NLB_3.01, NLB_4.01, NLB_4.02, NLB_5.01, NLB_7.01, and NLB_9.01. In another aspect, a corn plant, seed, or cell provided herein comprises NLB resistance QTL NLB_6.01 and three or more NLB resistance QTLs selected from the group consisting of NLB resistance QTLs NLB_2.01, NLB_3.01, NLB_4.01, NLB_4.02, NLB_5.01, NLB_7.01, and NLB_9.01. In another aspect, a corn plant, seed, or cell provided herein comprises NLB resistance QTL NLB_6.01 and four or more NLB resistance QTLs selected from the group consisting of NLB resistance QTLs NLB_2.01, NLB_3.01, NLB_4.01, NLB_4.02, NLB_5.01, NLB_7.01, and NLB_9.01. In another aspect, a corn plant, seed, or cell provided herein comprises NLB resistance QTL NLB_6.01 and five or more NLB resistance QTLs selected from the group consisting of NLB resistance QTLs NLB_2.01, NLB_3.01, NLB_4.01, NLB_4.02, NLB_5.01, NLB_7.01, and NLB_9.01. In another aspect, a corn plant, seed, or cell provided herein comprises NLB resistance QTL NLB_6.01 and or six or more NLB resistance QTLs selected from the group consisting of NLB resistance QTLs NLB_2.01, NLB_3.01, NLB_4.01, NLB_4.02, NLB_5.01, NLB_7.01, and NLB_9.01. In another aspect, a corn plant, seed, or cell provided herein comprises NLB resistance QTL NLB_6.01 and NLB resistance QTLs NLB_2.01, NLB_3.01, NLB_4.01, NLB_4.02, NLB_5.01, NLB_7.01, and NLB_9.01.
[0182] In another aspect, a corn plant, seed, or cell provided herein comprises NLB resistance QTL NLB_7.01 and one or more NLB resistance QTLs selected from the group consisting of NLB resistance QTLs NLB_2.01, NLB_3.01, NLB_4.01, NLB_4.02, NLB_5.01, NLB_6.01, and NLB_9.01. In another aspect, a corn plant, seed, or cell provided herein comprises NLB resistance QTL NLB_7.01 and two or more NLB resistance QTLs selected from the group consisting of NLB resistance QTLs NLB_2.01, NLB_3.01, NLB_4.01, NLB_4.02, NLB_5.01, NLB_6.01, and NLB_9.01. In another aspect, a corn plant, seed, or cell provided herein comprises NLB resistance QTL NLB_7.01 and three or more NLB resistance QTLs selected from the group consisting of NLB resistance QTLs NLB_2.01, NLB_3.01, NLB_4.01, NLB_4.02, NLB_5.01, NLB_6.01, and NLB_9.01. In another aspect, a corn plant, seed, or cell provided herein comprises NLB resistance QTL NLB_7.01 and four or more NLB resistance QTLs selected from the group consisting of NLB resistance QTLs NLB_2.01, NLB_3.01, NLB_4.01, NLB_4.02, NLB_5.01, NLB_6.01, and NLB_9.01. In another aspect, a corn plant, seed, or cell provided herein comprises NLB resistance QTL NLB_7.01 and five or more NLB resistance QTLs selected from the group consisting of NLB resistance QTLs NLB_2.01, NLB_3.01, NLB_4.01, NLB_4.02, NLB_5.01, NLB_6.01, and NLB_9.01. In another aspect, a corn plant, seed, or cell provided herein comprises NLB resistance QTL NLB_7.01 and or six or more NLB resistance QTLs selected from the group consisting of NLB resistance QTLs NLB_2.01, NLB_3.01, NLB_4.01, NLB_4.02, NLB_5.01, NLB_6.01, and NLB_9.01. In another aspect, a corn plant, seed, or cell provided herein comprises NLB resistance QTL NLB_7.01 and NLB resistance QTLs NLB_2.01, NLB_3.01, NLB_4.01, NLB_4.02, NLB_5.01, NLB_6.01, and NLB_9.01.
[0183] In another aspect, a corn plant, seed, or cell provided herein comprises NLB resistance QTL NLB_9.01 and one or more NLB resistance QTLs selected from the group consisting of NLB resistance QTLs NLB_2.01, NLB_3.01, NLB_4.01, NLB_4.02, NLB_5.01, NLB_6.01, and NLB_7.01. In another aspect, a corn plant, seed, or cell provided herein comprises NLB resistance QTL NLB_9.01 and two or more NLB resistance QTLs selected from the group consisting of NLB resistance QTLs NLB_2.01, NLB_3.01, NLB_4.01, NLB_4.02, NLB_5.01, NLB_6.01, and NLB_7.01. In another aspect, a corn plant, seed, or cell provided herein comprises NLB resistance QTL NLB_9.01 and three or more NLB resistance QTLs selected from the group consisting of NLB resistance QTLs NLB_2.01, NLB_3.01, NLB_4.01, NLB_4.02, NLB_5.01, NLB_6.01, and NLB_7.01. In another aspect, a corn plant, seed, or cell provided herein comprises NLB resistance QTL NLB_9.01 and four or more NLB resistance QTLs selected from the group consisting of NLB resistance QTLs NLB_2.01, NLB_3.01, NLB_4.01, NLB_4.02, NLB_5.01, NLB_6.01, and NLB_7.01. In another aspect, a corn plant, seed, or cell provided herein comprises NLB resistance QTL NLB_9.01 and five or more NLB resistance QTLs selected from the group consisting of NLB resistance QTLs NLB_2.01, NLB_3.01, NLB_4.01, NLB_4.02, NLB_5.01, NLB_6.01, and NLB_7.01. In another aspect, a corn plant, seed, or cell provided herein comprises NLB resistance QTL NLB_9.01 and or six or more NLB resistance QTLs selected from the group consisting of NLB resistance QTLs NLB_2.01, NLB_3.01, NLB_4.01, NLB_4.02, NLB_5.01, NLB_6.01, and NLB_7.01. In another aspect, a corn plant, seed, or cell provided herein comprises NLB resistance QTL NLB_9.01 and NLB resistance QTLs NLB_2.01, NLB_3.01, NLB_4.01, NLB_4.02, NLB_5.01, NLB_6.01, and NLB_7.01.
[0184] As an example, a corn plant or seed comprising one or more NLB resistance QTLs provided herein exhibits smaller leaf lesions compared to a corn plant or seed lacking the one or more NLB resistance QTLs under a high NLB stress condition. In an aspect, a corn plant or seed comprising one or more NLB resistance QTLs provided herein exhibits fewer leaf lesions compared to a corn plant or seed lacking the one or more NLB resistance QTLs under a high NLB stress condition. In an aspect, a corn plant or seed comprising one or more NLB resistance QTLs provided herein exhibits less leaf area covered by leaf lesions compared to a corn plant or seed lacking the one or more NLB resistance QTLs under a high NLB stress condition. In an aspect, a corn plant or seed comprising one or more NLB resistance QTLs provided herein exhibits reduced stem rot compared to a corn plant or seed lacking the one or more NLB resistance QTLs under a high NLB stress condition. In an aspect, a corn plant or seed comprising one or more NLB resistance QTLs provided herein exhibits reduced root rot compared to a corn plant or seed lacking the one or more NLB resistance QTLs under a high NLB stress condition. In an aspect, a corn plant or seed comprising one or more NLB resistance QTLs provided herein exhibits less foliage destruction compared to a corn plant or seed lacking the one or more NLB resistance QTLs under a high NLB stress condition.
[0185] In an aspect, this disclosure provides a method comprising providing a set of corn seeds described in any one of paragraphs [00175] to [00183] to a person desirous of planting the set of corn seeds in a field plot. In an aspect, a method comprising a field plot that exhibits NLB infection in any one of the previous one or more, two or more, three or more, four or more, five or more, six or more, seven or more, eight or more, nine or more, or ten or more planting seasons.
[0186] In an aspect, a method, a corn plant, or a corn seed provided herein is used in combination with one or more pesticides including, but not limited to, herbicides, fungicides (e.g., picoxystrobin, cyproconazole, tetraconazole, pyraclostrobin, metconazole, azoxystrobin, propiconazole, prothioconazole, trifloxystrobin), insecticides, microbiocides, nematicides, insect repellents, bactericides, and other substances used to control pests. In another aspect, a method, a corn plant, or a corn seed provided herein is used in combination with one or more triazoles, strobilurins, acylamino acids, pyrimidines, pyridines, arylphenyl ketones, amides, benzanilides, imidazoles, dinitrophenols, morpholines, phenylsulfamides and organophosphorus cpds, derivatives thereof and combinations thereof which can be applied as a seed treatment, a foliar treatment, a drench treatment, or a drip treatment.
[0187] In an aspect, corn seeds provided herein are untreated. In another aspect, corn seeds provided herein can be subjected to various and multiple treatments. For example, without being limiting, the seeds can be treated to improve germination by priming the seeds, by disinfection to protect against seed borne pathogens, or both priming and disinfection. In another example, seeds can be coated with any available coating to improve, for example, plantability, seed emergence, and protection against seed borne pathogens. Seed coating can be any form of seed coating including, but not limited to, pelleting, film coating, and encrustments.
[0188] In a further example, the disclosure provides methods to enhance NLB resistance by combining two or more, three or more, five or more, six or more, or seven or more NLB resistance QTLs provided herein. In an aspect, the combined NLB resistance QTLs have additive effects in providing NLB resistance. In another aspect, the combined NLB resistance QTLs have synergistic effects in providing NLB resistance. In a further aspect, the combination of two or more, three or more, four or more, five or more, six or more, or seven or more NLB resistance QTLs provided herein has no negative effects over corn physiology, resistance, yield, or performance in general.
[0189] In an aspect, this disclosure provides corn plant cells, tissues, and organs that are not reproductive material and do not mediate the natural reproduction of the plant. In one aspect, this disclosure provides a non-reproductive corn cell. In another aspect, this disclosure also provides corn plant cells, tissues, and organs that are reproductive material and mediate the natural reproduction of the plant. In another aspect, this disclosure provides corn plant cells, tissues, and organs that cannot maintain themselves via photosynthesis. In another aspect, this disclosure provides somatic corn plant cells. Somatic cells, contrary to germline cells, do not mediate plant reproduction.
[0190] As an example, the provided cells, tissues and organs can be from seed, fruit, leaf, leaf blade, leaf sheath, auricle, ligule, cotyledon, hypocotyl, meristem, embryos, endosperm, root, shoot, stem, pod, flower, inflorescence, stalk, pedicel, style, stigma, receptacle, petal, sepal, pollen, anther, filament, ovary, ovule, pericarp, phloem, bud, or vascular tissue. In another example, this disclosure provides a corn plant chloroplast or mitochondria. In a further example, this disclosure provides epidermal cells, stomata cell, trichomes, root hairs, a storage root, or a tuber. In another example, this disclosure provides a corn protoplast.
[0191] Skilled artisans understand that corn plants naturally reproduce via seeds, not via asexual reproduction or vegetative propagation. In an example, this disclosure provides corn endosperm. In another example, this disclosure provides corn endosperm cells. In a further example, this disclosure provides a male or female sterile corn plant, which cannot reproduce without human intervention.
[0192] In a further aspect, this disclosure provides processed products made from a provided corn plant, seed, or cell. As an example, such products include, but are not limited to, meal, oil, plant extract, starch, fermentation products, or digestion products. In another example, this disclosure also provides a corn meal, which is substantially oil free and which is produced using the oilseed of any of the plants provided herein. In another example, this disclosure also provides a method of providing a corn meal by crushing oilseed of any of the plants provided herein.
[0193] A corn plant, seed, or cell provided herein can also be genetically engineered to express various phenotypes of agronomic interest. Exemplary genes implicated in this regard include, but are not limited to, genes that confer resistance to pests or disease, genes that confer resistance or tolerance to an herbicide, genes that control male sterility, genes that affect abiotic stress resistance, and other genes and transcription factors that affect plant growth and agronomic traits such as yield, flowering, plant growth, or plant architecture.
Corn Transformation
[0194] A corn plant, seed, or cell provided herein can be genetically transformed. Numerous methods for plant transformation have been developed including biological and physical plant transformation protocols. See, for example, Mild et al., "Procedures for Introducing Foreign DNA into Plants" in Methods in Plant Molecular Biology and Biotechnology, Glick B. R. and Thompson, J. E. Eds. (CRC Press, Inc., Boca Raton, 1993) pages 67-88. In addition, expression vectors and in vitro culture methods for plant cell or tissue transformation and regeneration of plants are available. See, for example, Gruber et al., "Vectors for Plant Transformation" in Methods in Plant Molecular Biology and Biotechnology, Glick B. R. and Thompson, J. E. Eds. (CRC Press, Inc., Boca Raton, 1993) pages 89-119.
[0195] One method for introducing an expression vector into plants is based on the natural transformation system of Agrobacterium. See, e.g., Horsch et al., A Simple and General Method for Transferring Genes into Plants. Science, 227:1229-1231 (1985). A. tumefaciens and A. rhizogenes are plant pathogenic soil bacteria which genetically transform plant cells. Descriptions of Agrobacterium vector systems and methods for Agrobacterium-mediated gene transfer are provided by, for example, U.S. Pat. No. 5,563,055, incorporated herein by reference in its entirety.
[0196] Several methods of plant transformation, collectively referred to as direct gene transfer, have been developed as an alternative to Agrobacterium-mediated transformation. A generally applicable method of plant transformation is microprojectile-mediated transformation wherein DNA is carried on the surface of microprojectiles. The expression vector is introduced into plant tissues with a biolistic device that accelerates the microprojectiles to speeds of 300 to 600 m/s which is sufficient to penetrate plant cell walls and membranes.
[0197] Another method for physical delivery of DNA to plants is sonication of target cells. Alternatively, liposome and spheroplast fusion have been used to introduce expression vectors into plants. Electroporation of protoplasts and whole cells and tissues can also be used.
[0198] Following transformation of corn target tissues, expression of the above-described selectable marker genes allows for preferential selection of transformed cells, tissues, and/or plants, using regeneration and selection methods well-known in the art.
[0199] The foregoing methods for transformation would typically be used for producing a transgenic variety. The transgenic variety could then be crossed with another (non-transformed or transformed) variety, in order to produce a new transgenic variety. Alternatively, a genetic trait which has been engineered into a particular corn line using the foregoing transformation techniques could be moved into another line using traditional backcrossing techniques that are well-known in the plant breeding arts. For example, a backcrossing approach could be used to move an engineered trait from a public, non-elite variety into an elite variety, or from a variety containing a foreign gene in its genome into a variety or varieties which do not contain that gene.
[0200] A corn plant, seed, or cell provided herein can also be produced by one or more genome engineering techniques or subject to further genomic editing. For example, one or more NLB resistance alleles can be introduced into an NLB susceptible background. Exemplary genome engineering techniques include meganucleases, zinc-finger nucleases, TALENs, and CRISPR/Cas9 systems. See, e.g., Gaj et al., ZFN, TALEN, and CRISPR/Cas-based methods for genome engineering. Trends in Biotechnology, 31:397-405 (2013). Additional genome engineering techniques known to those of ordinary skill in the art are also envisioned.
Additional Breeding
[0201] A corn plant or seed provided herein can also be subject to additional breeding using one or more known methods in the art, e.g., pedigree breeding, recurrent selection, mass selection, and mutation breeding. Pedigree breeding starts with the crossing of two genotypes, such as a corn variety comprising an NLB resistance QTL or NLB resistance allele provided herein and another corn variety lacking such a locus. If the two original parents do not provide all the desired characteristics, other sources can be included in the breeding population. In the pedigree method, superior plants are selfed and selected in successive filial generations. In the succeeding filial generations the heterozygous condition gives way to homogeneous varieties as a result of self-fertilization and selection. Typically in the pedigree method of breeding, five or more successive filial generations of selfing and selection is practiced: F.sub.1 to F.sub.2; F.sub.2 to F.sub.3; F.sub.3 to F.sub.4; F.sub.4 to F.sub.5, etc. After a sufficient amount of inbreeding, successive filial generations will serve to increase seed of the developed variety. The developed variety can comprise homozygous alleles at about 95% or more of its loci.
[0202] In addition to being used to create a backcross conversion, backcrossing can also be used in combination with pedigree breeding. As discussed previously, backcrossing can be used to transfer one or more specifically desirable traits from one variety, the donor parent, to a developed variety called the recurrent parent, which has overall good agronomic characteristics yet lacks that desirable trait or traits. However, the same procedure can be used to move the progeny toward the genotype of the recurrent parent but at the same time retain many components of the non-recurrent parent by stopping the backcrossing at an early stage and proceeding with selfing and selection. For example, a corn variety can be crossed with another variety to produce a first generation progeny plant. The first generation progeny plant can then be backcrossed to one of its parent varieties to create a BC1 or BC2. Progenies are selfed and selected so that the newly developed variety has many of the attributes of the recurrent parent and yet several of the desired attributes of the non-recurrent parent. This approach leverages the value and strengths of the recurrent parent for use in new corn varieties.
[0203] Recurrent selection is a method used in a plant breeding program to improve a population of plants. The method entails individual plants cross pollinating with each other to form progeny. The progeny are grown and the superior progeny selected by any number of selection methods, which include individual plant, half-sib progeny, full-sib progeny and selfed progeny. The selected progeny are cross pollinated with each other to form progeny for another population. This population is planted and again superior plants are selected to cross pollinate with each other. Recurrent selection is a cyclical process and therefore can be repeated as many times as desired. The objective of recurrent selection is to improve the traits of a population. The improved population can then be used as a source of breeding material to obtain new varieties for commercial or breeding use, including the production of a synthetic line. A synthetic line is the resultant progeny formed by the intercrossing of several selected varieties.
[0204] Mass selection is another useful technique when used in conjunction with molecular marker enhanced selection. In mass selection, seeds from individuals are selected based on phenotype or genotype. These selected seeds are then bulked and used to grow the next generation. Bulk selection requires growing a population of plants in a bulk plot, allowing the plants to self-pollinate, harvesting the seed in bulk and then using a sample of the seed harvested in bulk to plant the next generation. Also, instead of self-pollination, directed pollination could be used as part of the breeding program.
[0205] Mutation breeding can also be used to introduce new traits into a corn plant or seed provided herein. Mutations that occur spontaneously or are artificially induced can be useful sources of variability for a plant breeder. The goal of artificial mutagenesis is to increase the rate of mutation for a desired characteristic. Mutation rates can be increased by many different means including temperature, long-term seed storage, tissue culture conditions, radiation (such as X-rays, gamma rays (e.g., cobalt-60 or cesium-137), neutrons (product of nuclear fission by uranium-235 in an atomic reactor), beta radiation (emitted from radioisotopes such as phosphorus-32 or carbon-14), or ultraviolet radiation (from 2500 to 2900 nm)), or chemical mutagens (such as base analogues (5-bromo-uracil), related compounds (8-ethoxy caffeine), antibiotics (streptonigrin), alkylating agents (sulfur mustards, nitrogen mustards, epoxides, ethylenamines, sulfates, sulfonates, sulfones, lactones), azide, hydroxylamine, nitrous acid, or acridines). Transposon- or T-DNA-based mutagenesis is also encompassed by the present disclosure. Once a desired trait is observed through mutagenesis the trait can then be incorporated into existing germplasm by traditional breeding techniques.
[0206] In an aspect, the disclosure provides a doubled haploid corn plant and seed that comprise an NLB resistance QTL or NLB resistance marker alleles provided herein. The doubled haploid approach achieves isogenic plants in a shorter time frame, and is particularly useful for generating inbred lines and quantitative genetics studies. Doubled haploid plants can be produced according to methods known in the art. For example, the initial step involves the haploidization of the plant which results in the production of a population comprising haploid seed. Non-homozygous lines are crossed with an inducer parent, resulting in the production of haploid seeds. Seeds that have haploid embryos, but normal triploid endosperm, advance to the second stage. After selecting haploid seeds from the population, the selected seeds undergo chromosome doubling to produce doubled haploid seeds. A spontaneous chromosome doubling in a cell lineage will lead to normal gamete production or the production of unreduced gametes from haploid cell lineages. Application of a chemical compound, such as colchicine, can be used to increase the rate of diploidization. Colchicine binds to tubulin and prevents its polymerization into microtubules, thus arresting mitosis at metaphase, can be used to increase the rate of diploidization, i.e. doubling of the chromosome number. These chimeric plants are self-pollinated to produce diploid (doubled haploid) seed. This doubled haploid seed is cultivated and subsequently evaluated and used in hybrid testcross production.
[0207] In an aspect, this disclosure also provides methods for making a substantially homozygous corn plant by producing or obtaining a seed from a cross of a corn plant comprising an NLB resistance allele and another corn plant and applying doubled haploid methods to the F.sub.1 seed or F.sub.1 plant or to any successive filial generation.
Hybrid Production
[0208] In an aspect, this disclosure provides a hybrid corn plant or seed, and their production. The development of a corn hybrid in a corn plant breeding program generally involves three steps: (1) the selection of plants from various germplasm pools for initial breeding crosses; (2) the selfing of the selected plants from the breeding crosses for several generations to produce a series of inbred lines, which, although different from each other, breed true and are highly uniform; and (3) crossing the selected inbred lines with different inbred lines to produce the hybrids. During the inbreeding process in corn, the vigor of the lines decreases. Vigor is restored when two different inbred lines are crossed to produce the hybrid. An important consequence of the homozygosity and homogeneity of the inbred lines is that the hybrid between a defined pair of inbreds will always be the same. Once the inbreds that give a superior hybrid have been identified, the hybrid seed can be reproduced indefinitely as long as the homogeneity of the inbred parents is maintained.
[0209] Combining ability of a line, as well as the performance of the line, is a factor in the selection of improved corn lines that can be used as inbreds. Combining ability refers to a line's contribution as a parent when crossed with other lines to form hybrids. The hybrids formed for the purpose of selecting superior lines are designated test crosses. One way of measuring combining ability is by using breeding values. Breeding values are based on the overall mean of a number of test crosses. This mean is then adjusted to remove environmental effects and it is adjusted for known genetic relationships among the lines.
[0210] Hybrid seed production requires inactivation of pollen produced by the female parent. A pollination control system and effective transfer of pollen from one parent to the other offers improved plant breeding and an effective method for producing hybrid corn seed and plants. For example, a male sterility system can be used to produce corn hybrids.
[0211] Male sterility genes can increase the efficiency with which hybrids are made, in that they eliminate the need to physically emasculate the plant used as a female in a given cross. Where one desires to employ male-sterility systems, it can be beneficial to also utilize one or more male-fertility restorer genes. For example, where cytoplasmic male sterility (CMS) is used, hybrid crossing requires three inbred lines: (1) a cytoplasmically male-sterile line having a CMS cytoplasm; (2) a fertile inbred with normal cytoplasm, which is isogenic with the CMS line for nuclear genes ("maintainer line"); and (3) a distinct, fertile inbred with normal cytoplasm, carrying a fertility restoring gene ("restorer" line). The CMS line is propagated by pollination with the maintainer line, with all of the progeny being male sterile, as the CMS cytoplasm is derived from the female parent. These male sterile plants can then be efficiently employed as the female parent in hybrid crosses with the restorer line, without the need for physical emasculation of the male reproductive parts of the female parent.
Marker Detection
[0212] In an aspect, the present disclosure provides markers that are in linkage disequilibrium with at least one NLB resistance QTL or NLB resistance allele and can be used to select for NLB resistance. Exemplary markers comprise SEQ ID NOs: 1-89 and 446-482 with their NLB resistance alleles shown in Table 4. Markers within approximately 20 cM, 15 cM, 10 cM, 5 cM, 4 cM, 3 cM, 2 cM, 1 cM, 0.5 cM or less than 0.5 cM of these exemplary markers can also be identified from the known art.
[0213] Genetic markers are distinguishable from each other (as well as from the plurality of alleles of any one particular marker) on the basis of polynucleotide length and/or sequence. In general, any differentially inherited polymorphic trait (including a nucleic acid polymorphism) that segregates among progeny is a potential genetic marker.
[0214] As a set, polymorphic markers serve as a useful tool for fingerprinting plants to inform the degree of identity of lines or varieties. These markers can form a basis for determining associations with phenotype and can be used to drive genetic gain. The implementation of marker-assisted selection is dependent on the ability to detect and analyze underlying genetic differences between individuals.
[0215] As an example, nucleic acid analysis methods include, but are not limited to, PCR-based detection methods, microarray methods, mass spectrometry-based methods, and/or nucleic acid sequencing methods. In an aspect, the detection of polymorphic sites in a sample of DNA, RNA, or cDNA can be facilitated through the use of nucleic acid amplification methods. Such methods specifically increase the concentration of polynucleotides that span the polymorphic site, or include that site and sequences located either distal or proximal to it. Such amplified molecules can be readily detected by gel electrophoresis, fluorescence detection methods, or other means.
[0216] A method of achieving such amplification employs the polymerase chain reaction (PCR) using primer pairs that are capable of hybridizing to the proximal sequences that define a polymorphism in its double-stranded form. Methods for typing DNA based on mass spectrometry have been provided in U.S. Pat. Nos. 6,613,509 and 6,503,710, and references found therein.
[0217] Polymorphisms in DNA sequences can be detected or typed by a variety of effective methods well known in the art including, but not limited to, those provided in U.S. Pat. Nos. 5,468,613, 5,217,863; 5,210,015; 5,876,930; 6,030,787; 6,004,744; 6,013,431; 5,595,890; 5,762,876; 5,945,283; 5,468,613; 6,090,558; 5,800,944; 5,616,464; 7,312,039; 7,238,476; 7,297,485; 7,282,355; 7,270,981; and 7,250,252 all of which are incorporated herein by reference in their entireties. However, the compositions and methods of the present disclosure can be used in conjunction with any polymorphism typing method to type polymorphisms in genomic DNA samples. These genomic DNA samples used include but are not limited to genomic DNA isolated directly from a plant, cloned genomic DNA, or amplified genomic DNA.
[0218] For instance, polymorphisms in DNA sequences can be detected by hybridization to allele-specific oligonucleotide (ASO) probes as provided in U.S. Pat. Nos. 5,468,613 and 5,217,863. U.S. Pat. No. 5,468,613 discloses allele specific oligonucleotide hybridizations where single or multiple nucleotide variations in nucleic acid sequence can be detected in nucleic acids by a process in which the sequence containing the nucleotide variation is amplified, spotted on a membrane and treated with a labeled sequence-specific oligonucleotide probe.
[0219] Target nucleic acid sequence can also be detected by probe ligation methods as provided in U.S. Pat. No. 5,800,944 where sequence of interest is amplified and hybridized to probes followed by ligation to detect a labeled part of the probe.
[0220] Microarrays can also be used for polymorphism detection, wherein oligonucleotide probe sets are assembled in an overlapping fashion to represent a single sequence such that a difference in the target sequence at one point would result in partial probe hybridization (Borevitz et al., Large-scale identification of single-feature polymorphisms in complex genomes. Genome Research, 13:513-523 (2003); Cui et al., Detecting single-feature polymorphisms using oligonucleotide array and robustified projection pursuit. Bioinformatics, 21:3852-3858 (2005)). On any one microarray, it is expected there will be a plurality of target sequences, which can represent genes and/or noncoding regions wherein each target sequence is represented by a series of overlapping oligonucleotides, rather than by a single probe. This platform provides for high throughput screening a plurality of polymorphisms. A single-feature polymorphism (SFP) is a polymorphism detected by a single probe in an oligonucleotide array, wherein a feature is a probe in the array. Typing of target sequences by microarray-based methods is provided in U.S. Pat. Nos. 6,799,122; 6,913,879; and 6,996,476.
[0221] Target nucleic acid sequence can also be detected by probe linking methods as provided in U.S. Pat. No. 5,616,464, employing at least one pair of probes having sequences homologous to adjacent portions of the target nucleic acid sequence and having side chains which non-covalently bind to form a stem upon base pairing of the probes to the target nucleic acid sequence. At least one of the side chains has a photoactivatable group which can form a covalent cross-link with the other side chain member of the stem.
[0222] Other exemplary methods for detecting SNPs and Indels include single base extension (SBE) methods. Examples of SBE methods include, but are not limited, to those provided in U.S. Pat. Nos. 6,004,744; 6,013,431; 5,595,890; 5,762,876; and 5,945,283. SBE methods are based on extension of a nucleotide primer that is adjacent to a polymorphism to incorporate a detectable nucleotide residue upon extension of the primer. In an aspect, the SBE method uses four synthetic oligonucleotides. Two of the oligonucleotides serve as PCR primers and are complementary to sequence of the locus of genomic DNA which flanks a region containing the polymorphism to be assayed. Following amplification of the region of the genome containing the polymorphism, the PCR product is mixed with the third and fourth oligonucleotides (called extension primers) which are designed to hybridize to the amplified DNA adjacent to the polymorphism in the presence of DNA polymerase and two differentially labeled dideoxynucleosidetriphosphates. If the polymorphism is present on the template, one of the labeled dideoxynucleosidetriphosphates can be added to the primer in a single base chain extension. The allele present is then inferred by determining which of the two differential labels was added to the extension primer. Homozygous samples will result in only one of the two labeled bases being incorporated and thus only one of the two labels will be detected. Heterozygous samples have both alleles present, and will thus direct incorporation of both labels (into different molecules of the extension primer) and thus both labels will be detected.
[0223] In another exemplary method for detecting polymorphisms, SNPs and indels can be detected by methods provided in U.S. Pat. Nos. 5,210,015; 5,876,930; and 6,030,787 in which an oligonucleotide probe having a 5' fluorescent reporter dye and a 3' quencher dye covalently linked to the 5' and 3' ends of the probe. When the probe is intact, the proximity of the reporter dye to the quencher dye results in the suppression of the reporter dye fluorescence, e.g., by Forster-type energy transfer. During PCR, forward and reverse primers hybridize to a specific sequence of the target DNA flanking a polymorphism while the hybridization probe hybridizes to polymorphism-containing sequence within the amplified PCR product. In the subsequent PCR cycle DNA polymerase with 5'-3' exonuclease activity cleaves the probe and separates the reporter dye from the quencher dye resulting in increased fluorescence of the reporter.
[0224] As an example, the locus or loci of interest can be directly sequenced using nucleic acid sequencing technologies. Methods for nucleic acid sequencing are known in the art and include technologies provided by 454 Life Sciences (Branford, Conn.), Agencourt Bioscience (Beverly, Mass.), Applied Biosystems (Foster City, Calif.), LI-COR Biosciences (Lincoln, Nebr.), NimbleGen Systems (Madison, Wis.), Illumina (San Diego, Calif.), Pac-Bio (Menlo Park, Calif.) and VisiGen Biotechnologies (Houston, Tex.). Such nucleic acid sequencing technologies comprise formats such as parallel bead arrays, sequencing by ligation, capillary electrophoresis, electronic microchips, "biochips," microarrays, parallel microchips, and single-molecule arrays, as reviewed by Service, Gene sequencing: the race for the $1000 genome. Science, 311:1544-46 (2006).
[0225] As an example, in silico methods can be used to detect the marker loci of interest. For example, the sequence of a nucleic acid comprising the marker locus of interest can be stored in a computer. The desired marker locus sequence or its homolog can be identified using an appropriate nucleic acid search algorithm as provided by, for example, in such readily available programs as BLAST, or even simple word processors.
[0226] In an aspect, any of the aforementioned marker types can be employed in the context of this disclosure to identify chromosome intervals encompassing a genetic element that contributes to superior agronomic performance (e.g., corn NLB resistance).
[0227] The markers to be used in the methods of the present disclosure should preferably be diagnostic of origin in order for inferences to be made about subsequent populations. Experience to date suggests that SNP markers can be ideal for mapping because the likelihood that a particular SNP allele is derived from independent origins in the extant populations of a particular species is very low. As such, SNP markers appear to be useful for tracking and assisting introgression of QTL, particularly in the case of genotypes.
Association Mapping
[0228] In an aspect, the present disclosure also provides chromosome intervals, marker loci, germplasm for conducting genome-wide association mapping for NLB resistance. Exemplary chromosome intervals and marker loci are provided in Tables 4 and 6. Genome-wide association mapping is conducted to find signals of association for various complex traits by surveying genetic variation in the whole genome.
[0229] Association mapping relies on chromosomal recombination opportunities over a large number of generations, in the history of a species, which allows the removal of association between a QTL and any marker not tightly linked to it, thus improving the rate of discovery of true association (Jannink and Walsh, Quantitative Genetics, Genomics and Plant Breeding, Kang, Ed. CAB International, pp. 59-68 (2002)).
[0230] An approach used to link phenotypic variation with genetic loci is marker-trait association (MTA) mapping, also known as linkage disequilibrium (LD) mapping. LD mapping emerged as an important gene mapping tool in the early 1990's with the advent of high-throughput genotyping technology, and has been widely used in human genetics to identify genes affecting human diseases. This approach was introduced and began to be adopted in plant gene mapping studies in early 2000's (Flint-Garcia et al., Structure of linkage disequilibrium in plants. Annual Review of Plant Biology, 54:357-374 (2003)).
[0231] LD mapping assumes that the main cause for LD is linkage that binds loci on the same chromosome together in transmission to next generation. However, due to recombination events accumulated over many generations in a natural population, each chromosome has been shuffled deeply, so that the chromosome has been broken into many tiny regions where loci remain transmitted together, but loci from different regions tend to transmit independently as if they were from different chromosomes. Chromosomal regions where loci are bound together in transmission are commonly known as LD blocks (Reich et al., Linkage disequilibrium in the human genome. Nature, 411:199-204 (2001)). LD mapping identifies genes of interest through genetic markers on the LD blocks where the genes are located. This is done by detecting significant associations between the markers and the traits that the genes affect with a sample of unrelated individuals or a sample of unrelated pedigrees that are genotyped on a selected set of markers covering candidate gene regions or the whole genome, and phenotyped on a set of traits of interest.
[0232] Compared with traditional linkage mapping methods that are typically based on artificial biparental segregating populations (e.g., F.sub.2, BC, doubled haploid, recombinant inbred line, etc.), LD mapping generally produces better mapping resolution, because of the smaller sizes of LD blocks. In addition, LD mapping is useful in identifying more than two functional alleles at associated markers in a germplasm. Further, LD mapping is efficient for evaluating natural populations.
Identification of QTLs
[0233] As an example, markers, alleles, and haplotypes provided herein can be used for identifying QTLs associated with NLB resistance. The statistical principles of QTL identification include penalized regression analysis, ridge regression, single marker analysis, complex pedigree analysis, Bayesian MCMC, identity-by-descent analysis, interval mapping, composite interval mapping (CIM), joint linkage mapping, and Haseman-Elston regression.
[0234] A QTL can act through a single gene mechanism or by a polygenic mechanism. In an aspect, the present disclosure provides an NLB resistance QTL interval, where an NLB resistance QTL (or multiple NLB resistance QTLs) that segregates with an NLB resistance trait is contained in the chromosomal interval. As used herein, when a QTL (or multiple QTLs) segregates with the NLB resistance trait, it is referred to herein as an "NLB resistance locus" (or "NLB resistance loci").
[0235] In an aspect of this disclosure, the boundaries of an NLB resistance QTL interval are drawn to encompass markers that will be closely linked to or associated with one or more NLB resistance QTLs. In other words, an NLB resistance QTL interval is drawn such that any marker that lies within that interval (including the terminal markers that define the boundaries of the interval) is genetically linked to or associated with the NLB resistance QTL. Each interval comprises at least one NLB resistance QTL, and furthermore, can indeed comprise more than one NLB resistance QTL. Close proximity of multiple QTLs in the same interval can obfuscate the correlation of a particular marker with a particular QTL, as one marker can demonstrate linkage to more than one QTL. Conversely, e.g., if two markers in close proximity show co-segregation with the desired phenotypic trait, it is sometimes unclear if each of those markers identifying the same QTL or two different QTLs. Regardless, knowledge of how many QTLs are in a particular interval is not necessary to make or practice the claimed subject matter.
[0236] As an example, the present disclosure also provides the mapping of additional SNP markers associated with or closely linked to one or more NLB resistance QTLs provided herein. SNP markers are ideal for mapping because the likelihood that a particular SNP allele is derived from independent origins in the extant populations of a particular species is very low. As such, SNP markers are useful for tracking and assisting introgression of NLB resistance QTLs, particularly in the case of haplotypes. In an aspect, a SNP marker is selected for mapping an NLB resistance QTL based on the marker's genetic map position. In another aspect, a SNP marker is selected for mapping an NLB resistance QTL based on the marker's physical map position.
[0237] The genetic linkage of additional marker molecules can be established by a gene mapping model such as, without limitation, the flanking marker model reported by Lander and Botstein, (Lander and Botstein, Mapping Mendelian Factors Underlying Quantitative Traits Using RFLP Linkage Maps. Genetics, 121:185-199 (1989)), and the interval mapping, based on maximum likelihood methods described by Lander and Botstein (supra), and implemented in the software package MAPMAKER/QTL (Lincoln and Lander, Mapping Genes Controlling Quantitative Traits Using MAPMAKER/QTL, Whitehead Institute for Biomedical Research, Massachusetts, (1990). Additional software includes Qgene, Version 2.23 (1996), Department of Plant Breeding and Biometry, 266 Emerson Hall, Cornell University, Ithaca, N.Y., the manual of which is herein incorporated by reference in its entirety).
[0238] A maximum likelihood estimate (MLE) for the presence of a marker is calculated, together with an MLE assuming no QTL effect, to avoid false positives. A log.sub.10 of an odds ratio (LOD) is then calculated as: LOD=log.sub.10 (MLE for the presence of a QTL/MLE given no linked QTL). The LOD score essentially indicates how much more likely the data are to have arisen assuming the presence of a QTL versus in its absence. The LOD threshold value for avoiding a false positive with a given confidence, say 95%, depends on the number of markers and the length of the genome. Graphs indicating LOD thresholds are set forth in Lander and Botstein, (Lander and Botstein, Mapping Mendelian Factors Underlying Quantitative Traits Using RFLP Linkage Maps. Genetics, 121:185-199 (1989), and further described by Ar s and Moreno-Gonzilez, Plant Breeding, Hayward, Bosemark, Romagosa (eds.) Chapman & Hall, London, pp. 314-331 (1993).
[0239] Additional models can be used. Many modifications and alternative approaches to interval mapping have been reported, including the use of non-parametric methods (Kruglyak and Lander, A Nonparametric Approach for Mapping Quantitative Trait Loci. Genetics, 139:1421-1428 (1995), the entirety of which is herein incorporated by reference). Multiple regression methods or models can be also be used, in which the trait is regressed on a large number of markers (Jansen, Biometrics in Plant Breed, van Oijen, Jansen (eds.) Proceedings of the Ninth Meeting of the Eucarpia Section Biometrics in Plant Breeding, The Netherlands, pp. 116-124 (1994); Weber and Wricke, Advances in Plant Breeding, Blackwell, Berlin, 16 (1994)). Procedures combining interval mapping with regression analysis, whereby the phenotype is regressed onto a single putative QTL at a given marker interval, and at the same time onto a number of markers that serve as `cofactors,` have been reported by Jansen and Stam, High Resolution of Quantitative Traits Into Multiple Loci via Interval Mapping. Genetics, 136:1447-1455 (1994) and Zeng, Precision Mapping of Quantitative Trait Loci. Genetics, 136:1457-1468 (1994). Generally, the use of cofactors reduces the bias and sampling error of the estimated QTL positions (Utz and Melchinger, Biometrics in Plant Breeding, van Oijen, Jansen (eds.) Proceedings of the Ninth Meeting of the Eucarpia Section Biometrics in Plant Breeding, The Netherlands, pp. 195-204 (1994)), thereby improving the precision and efficiency of QTL mapping (Zeng, Precision Mapping of Quantitative Trait Loci. Genetics, 136:1457-1468 (1994)). These models can be extended to multi-environment experiments to analyze genotype-environment interactions (Jansen et al., Genotype-by-environment interaction in genetic mapping of multiple quantitative trait loci. Theoretical and Applied Genetics, 91:33-37 (1995)).
[0240] In an aspect, this disclosure provides chromosomal intervals comprising QTL associated with NLB resistance. In an aspect, the chromosome intervals of this disclosure are characterized by genomic regions including and flanked by any two of marker loci SEQ ID NOs: 12 to 15. In another aspect, the chromosome intervals of this disclosure are characterized by genomic regions including and flanked by any two of marker loci SEQ ID NOs: 22 to 25. In another aspect, the chromosome intervals of this disclosure are characterized by genomic regions including and flanked by any two of marker loci SEQ ID NOs: 37 to 42 and 474. In another aspect, the chromosome intervals of this disclosure are characterized by genomic regions including and flanked by any two of marker loci SEQ ID NOs: 44 to 49. In another aspect, the chromosome intervals of this disclosure are characterized by genomic regions including and flanked by any two of marker loci SEQ ID NOs: 57 to 62 and 458 to 466. In another aspect, the chromosome intervals of this disclosure are characterized by genomic regions including and flanked by any two of marker loci SEQ ID NOs: 79 to 81. In another aspect, the chromosome intervals of this disclosure are characterized by genomic regions including and flanked by any two of marker loci SEQ ID NOs: 87 to 89, 477, and 480. In another aspect, the chromosome intervals of this disclosure are characterized by genomic regions including and flanked by the marker loci SEQ ID NOs: 469 and 470.
[0241] In an aspect, this disclosure provides chromosomal intervals comprising QTL associated with NLB resistance. In an aspect, the chromosome intervals of this disclosure are characterized by genomic regions including and flanked by any two of marker loci SEQ ID NOs: 8 to 18. In another aspect, the chromosome intervals of this disclosure are characterized by genomic regions including and flanked by any two of marker loci SEQ ID NOs: 21 to 29. In another aspect, the chromosome intervals of this disclosure are characterized by genomic regions including and flanked by any two of marker loci SEQ ID NOs: 33 to 42, 473, and 474. In another aspect, the chromosome intervals of this disclosure are characterized by genomic regions including and flanked by any two of marker loci SEQ ID NOs: 43 to 49 and 475. In another aspect, the chromosome intervals of this disclosure are characterized by genomic regions including and flanked by any two of marker loci SEQ ID NOs: 57 to 64 and 458 to 468. In another aspect, the chromosome intervals of this disclosure are characterized by genomic regions including and flanked by any two of marker loci SEQ ID NOs: 74 to 82. In another aspect, the chromosome intervals of this disclosure are characterized by genomic regions including and flanked by any two of marker loci SEQ ID NOs: 86 to 89, 476, 477, 479, and 480.
[0242] This disclosure also provides multiple markers linked to or associated with an NLB resistance QTL, for example, the markers having the sequence selected from SEQ ID NOs: 1-89 and 446-482. This disclosure therefore provides plants comprising a nucleic acid molecule selected from the group consisting of SEQ ID NOs: 1-89 and 446-482, fragments thereof, or complements thereof. The present disclosure further provides a plant comprising alleles of the chromosome interval linked to or associated with NLB resistance or fragments and complements thereof as well as any plant comprising any combination of two or more NLB resistance alleles of marker loci selected from the group consisting of SEQ ID NOs: 1-89 and 446-482. Plants provided by this disclosure can be homozygous or heterozygous for such alleles.
[0243] The compositions and methods of the present disclosure can be utilized to guide MAS or breeding corn varieties with a desired complement (set) of allelic forms of chromosome intervals associated with superior agronomic performance (e.g., NLB resistance). Any of the provided marker alleles can be introduced into a corn line via introgression, by traditional breeding (or introduced via transformation, or both) to yield a corn plant with superior agronomic performance. The number of alleles associated with NLB resistance that can be introduced or be present in a corn plant of the present disclosure ranges from 1 to the number of alleles provided herein, each integer of which is incorporated herein as if explicitly recited.
[0244] MAS using additional markers flanking either side of the DNA locus provide further efficiency because an unlikely double recombination event would be needed to simultaneously break linkage between the locus and both markers. Moreover, using markers tightly flanking a locus, one skilled in the art of MAS can reduce linkage drag by more accurately selecting individuals that have less of the potentially deleterious donor parent DNA. Any marker linked to or among the chromosome intervals described herein can thus find use within the scope of this disclosure.
[0245] These marker loci can be introgressed into any desired genomic background, germplasm, plant, line, variety, etc., as part of an overall MAS breeding program designed to enhance NLB resistance. This disclosure also provides QTL intervals that can be used in MAS to select plants that demonstrate NLB resistance. Similarly, QTL intervals can also be used to counter-select plants that are lacking NLB resistance. By identifying plants lacking a desired marker locus, plants lacking NLB resistance can be identified and selected or eliminated from subsequent crosses.
[0246] The present disclosure also extends to a method of making a progeny corn plant and the resulting progeny corn plants. In an aspect, the method comprises crossing a first parent corn plant with a second corn plant and growing the corn plant parent under plant growth conditions to yield corn plant progeny. Methods of crossing and growing a corn plant are well within the ability of those of ordinary skill in the art. Such corn plant progeny can be assayed for alleles associated with NLB resistance as provided herein and, thereby, the desired progeny selected. Such progeny plants or seed thereof can be sold commercially for corn production, used for food, processed to obtain a desired constituent of the corn, or further utilized in subsequent rounds of breeding. At least one of the first or second corn plants can be a corn plant of the present disclosure in that it comprises at least one of the allelic forms of the markers of the present disclosure, such that the progeny are capable of inheriting the allele.
[0247] By providing the positions in the corn genome of QTL intervals and the associated markers within those intervals, this disclosure also allows one skilled in the art to identify and use other markers within the intervals provided herein or linked to or associated with the intervals provided herein. Having identified such markers, these intervals can be readily identified from public linkage maps.
[0248] Closely linked markers flanking the locus of interest that have alleles in linkage disequilibrium (LD) with an NLB resistance allele at that locus can be effectively used to select for progeny plants with NLB resistance. Thus, the markers described herein, such as those listed in Table 4, as well as other markers genetically linked to or associated with the same chromosome interval, can be used to select for a corn plant, seed, or cell with NLB resistance. Often, a set of these markers will be used, (e.g., 2 or more, 3 or more, 4 or more, 5 or more) in the flanking regions of the locus. Optionally, as described above, a marker flanking or within the actual locus can also be used. The parents and their progeny can be screened for these sets of markers, and the markers that are polymorphic between the two parents used for selection. In an introgression program, this allows for selection of the gene or locus genotype at the more proximal polymorphic markers and selection for the recurrent parent genotype at the more distal polymorphic markers.
[0249] The choice of markers actually used to practice this disclosure is not limited and can be any marker that is genetically linked to or associated with the QTL intervals as described in Table 6, including markers within approximately 20 cM, 15 cM, 10 cM, 5 cM, 4 cM, 3 cM, 2 cM, 1 cM, 0.5 cM or less than 0.5 cM of the intervals provided herein. Examples include, but are not limited to, any marker selected from SEQ ID NOs: 1-89 and 446-482. In an aspect, a marker locus selected from SEQ ID NOs: 1-89 and 446-482 can be amplified using an appropriate pair of primers as indicated in Table 5. Furthermore, since there are many different types of marker detection assays known in the art, it is not intended that the type of marker detection assay used to practice this disclosure be limited in any way.
Marker Assisted Selection (MAS) Breeding
[0250] Marker loci and their NLB resistance alleles provided herein can be used in MAS breeding of NLB resistance. The more tightly linked a marker is with a DNA locus influencing a phenotype (e.g., NLB resistance), the more reliable the marker is in MAS, as the likelihood of a recombination event unlinking the marker and the locus decreases. Markers containing the causal mutation for a trait, or that are within the coding sequence of a causative gene, are ideal as no recombination is expected between them and the sequence of DNA responsible for the phenotype. However, markers do not need to contain or correspond to causal mutations in order to be effective in MAS. In fact, most MAS breeding only uses markers linked to or associated with a causal mutation.
[0251] Developing molecular markers in crop species can increase efficiency in plant breeding through MAS. Genetic markers are used to identify plants that contain a desired genotype at one or more loci, and that are expected to transfer the desired genotype, along with a desired phenotype to their progeny. Genetic markers can be used to identify plants containing a desired genotype at one locus, or at several unlinked or linked loci (e.g., a haplotype), and that would be expected to transfer the desired genotype, along with a desired phenotype to their progeny. The present disclosure provides the means to identify plants that exhibit NLB resistance by identifying chromosomal intervals and genetic markers associated with NLB resistance.
[0252] In general, MAS uses polymorphic markers that have been identified as having a significant likelihood of co-segregation with a desired trait. Such markers are presumed to map near a gene or genes that give the plant its desired phenotype, and are considered indicators for the desired trait.
[0253] Identification of plants or germplasm that include a marker locus or marker loci linked to a desired trait or traits provides a basis for performing MAS. Plants that comprise favorable markers or favorable alleles are selected for, while plants that comprise markers or alleles that are negatively correlated with the desired trait can be selected against. Desired markers and/or alleles can be introgressed into plants having a desired (e.g., elite or exotic) genetic background to produce an introgressed plant or germplasm having the desired trait. In an aspect, it is contemplated that a plurality of markers for desired traits are sequentially or simultaneous selected and/or introgressed. The combinations of markers that are selected for in a single plant is not limited, and can include any combination of markers provided herein or any marker linked to the markers provided herein, or any markers located within the QTL intervals defined herein.
[0254] In an aspect, a first corn plant or germplasm exhibiting a desired trait (the donor, e.g., an NLB resistant corn plant) can be crossed with a second corn plant or germplasm (the recipient; e.g., an elite or exotic corn, depending on characteristics that are desired in the progeny) to create an introgressed corn plant or germplasm as part of a breeding program. In an aspect, the recipient plant can also contain one or more loci associated with one or more desired traits, which can be qualitative or quantitative trait loci. In another aspect, the recipient plant can contain a transgene.
[0255] In an aspect, the recipient corn plant or germplasm will typically lack desired traits as compared to the first corn plant or germplasm, while the introgressed corn plant or germplasm will display improved traits as compared to the second plant or germplasm. An introgressed corn plant or germplasm produced by these methods are also a feature of this disclosure.
[0256] MAS is a powerful shortcut to select for desired phenotypes and for introgressing desired traits into cultivars (e.g., introgressing desired traits into elite lines). MAS is easily adapted to high throughput molecular analysis methods that can quickly screen large numbers of plant or germplasm genetic material for the markers of interest and is much more cost effective than cultivating and observing plants for visible traits.
Genomic Selection
[0257] Genomic selection (GS), also known as genome wide selection (GWS), is a form of MAS that estimates all locus, haplotype, and/or marker effects across the entire genome to calculate genomic estimated breeding values (GEBVs). See Nakaya and Isobe, Will genomic selection be a practical method for plant breeding? Annals of Botany 110: 1303-1316 (2012); Van Vleck et al., Estimated breeding values for meat characteristics of cross-bred cattle with an animal model. Journal of Animal Science 70: 363-371 (1992); and Heffner et al., Genomic selection for crop improvement. Crop Science 49: 1-12 (2009). GS utilizes a training phase and a breeding phase. In the training phase, genotypes and phenotypes are analyzed in a subset of a population to generate a GS prediction model that incorporates significant relationships between phenotypes and genotypes. A GS training population must be representative of selection candidates in the breeding program to which GS will be applied. In the breeding phase, genotype data are obtained in a breeding population, then favorable individuals are selected based on GEBVs obtained using the GS prediction model generated during the training phase without the need for phenotypic data.
[0258] Larger training populations typically increase the accuracy of GEBV predictions. Increasing the training population to breeding population ratio is helpful for obtaining accurate GEBVs when working with populations having high genetic diversity, small breeding populations, low heritability of traits, or large numbers of QTLs. The number of markers required for GS modeling is determined based on the rate of LD decay across the genome, which must be calculated for each specific population to which GS will be applied. In general, more markers will be necessary with faster raters of LD decay. Ideally, GS comprises at least one marker in LD with each QTL, but in practical terms one of ordinary skill in the art would recognized that this is not necessary.
[0259] With genotyping data, favorable individuals from a population can be selected based only on GEBVs. GEBVs are the sum of the estimate of genetic deviation and the weighted sum of estimates of breed effects, which are predicted using phenotypic data. Without being limiting, commonly used statistical models for prediction of GEBVs include best linear unbiased prediction (Henderson, Best linear unbiased estimation and prediction under a selection model. Biometrics 31: 423 (1975)) and a Bayesian framework (Gianola and Fernando, Bayesian methods in animal breeding theory. Journal of Animal Science 63: 217-244 (1986)).
[0260] The compositions and methods of the present disclosure can be utilized for GS or breeding corn varieties with a desired complement (set) of allelic forms of chromosome intervals associated with superior agronomic performance (e.g., NLB resistance). In an aspect, a corn plant, seed, or cell provided herein can be selected using genomic selection. In another aspect, SEQ ID NOs: 1-89 and 446-482 can be used in a method comprising genomic selection. In another aspect, a genomic selection method provided herein comprises phenotyping a population of corn plants for NLB resistance using the NLB rating scale provided in Table 1. In another aspect, a genomic selection method provided herein comprises genotyping a population of corn plants, seeds, or cells with at least one of marker loci SEQ ID NOs: 1-89 and 446-482.
Introgression of NLB Resistance QTLs Using MAS
[0261] The disclosure provides methods and markers for introgressing one or more NLB resistance QTLs provided herein into a new corn variety using MAS.
[0262] Multiple methods are available to achieve the introgression. For example, introgression of a desired allele at a specified locus can be transmitted to at least one progeny via a cross between two parents of the same species, where at least one of the parents has the desired allele in its genome. Alternatively, for example, transmission of an allele can occur by recombination between two donor genomes, e.g., in a fused protoplast, where at least one of the donor protoplasts has the desired allele in its genome. The desired allele can be, e.g., a selected allele of a marker, a QTL, a transgene, or the like. In any case, offspring comprising the desired allele can be repeatedly backcrossed to a line having a desired genetic background and selected for the desired allele, to result in the allele becoming fixed in a selected genetic background.
[0263] The introgression of one or more desired loci from a donor line into another line is achieved via repeated backcrossing to a recurrent parent accompanied by selection to retain one or more loci from the donor parent. Markers associated with NLB resistance are assayed in progeny and those progeny with one or more desired markers are selected for advancement. In another aspect, one or more markers can be assayed in the progeny to select for plants with the genotype of the agronomically elite parent.
[0264] It is generally anticipated that trait introgression activities will require more than one generation, wherein progeny are crossed to the recurrent (agronomically elite) parent or selfed. Selections are made based on the presence of one or more markers linked to NLB resistance and can also be made based on the recurrent parent genotype, wherein screening is performed on a genetic marker and/or phenotype basis. In another aspect, markers of this disclosure can be used in conjunction with other markers, ideally at least one on each chromosome of the corn genome, to track the introgression of NLB resistance into elite germplasm. In another aspect, QTL intervals associated with NLB resistance will be useful in conjunction with SNP molecular markers of the present disclosure to combine quantitative and qualitative NLB resistance in the same plant. It is within the scope of this disclosure to utilize the methods and compositions for trait integration of NLB resistance. It is contemplated by the inventors that the present disclosure will be useful for developing commercial varieties with NLB resistance and other agronomically elite phenotypes.
[0265] The following non-limiting embodiments are envisioned: [0266] 1. A method of creating a population of corn plants, seeds, or cells, said method comprising: [0267] a. genotyping a first population of corn plants, seeds, or cells at one or more marker loci associated with and within about 10 cM of one or more Northern Leaf Blight (NLB) resistance quantitative trait loci (QTLs) selected from the group consisting of NLB resistance QTLs NLB_2.01, NLB_3.01, NLB_4.01, NLB_4.02, NLB_5.01, NLB_6.01, NLB_7.01, and NLB_9.01; [0268] b. selecting from said first population one or more corn plants, seeds, or cells comprising one or more NLB resistance alleles of said one or more marker loci; and [0269] c. producing from said selected one or more corn plants, seeds, or cells a second population of corn plants, seeds, or cells comprising said one or more NLB QTLs. [0270] 2. The method of embodiment 1, wherein said one or more marker loci are located in a chromosomal interval flanked by: [0271] any two of marker loci SEQ ID NOs: 1 to 18; [0272] any two of marker loci SEQ ID NOs: 19 to 31; [0273] any two of marker loci SEQ ID NOs: 32 to 52 and 471 to 475; [0274] any two of marker loci SEQ ID NOs: 53 to 65 and 446 to 468; [0275] any two of marker loci SEQ ID NOs: 66 to 84; [0276] any two of marker loci SEQ ID NOs: 85 to 89 and 476 to 482; or [0277] marker loci SEQ ID NOs: 469 and 470. [0278] 3. The method of embodiments 1 or 2, wherein said one or more marker loci are located in a chromosomal interval flanked by: [0279] any two marker loci selected from the group consisting of SEQ ID NOs: 12 to 15; [0280] any two marker loci selected from the group consisting of SEQ ID NOs: 22 to 25; [0281] any two marker loci selected from the group consisting of SEQ ID NOs: 37 to 42 and 474; [0282] any two marker loci selected from the group consisting of SEQ ID NOs: 44 to 49; [0283] any two marker loci selected from the group consisting of SEQ ID NOs: 57 to 62 and 458 to 466; [0284] any two marker loci selected from the group consisting of SEQ ID NOs: 79 to 81; [0285] any two marker loci selected from the group consisting of SEQ ID NOs: 87 to 89, 477, and 480; or [0286] marker loci SEQ ID NOs: 469 and 470. [0287] 4. The method of embodiments 1 or 2, wherein said one or more marker loci are located in a chromosomal interval flanked by: [0288] any two marker loci selected from the group consisting of SEQ ID NOs: 8 to 18; [0289] any two marker loci selected from the group consisting of SEQ ID NOs: 21 to 29; [0290] any two marker loci selected from the group consisting of SEQ ID NOs: 33 to 42, 473, and 474; [0291] any two marker loci selected from the group consisting of SEQ ID NOs: 43 to 49 and 475; [0292] any two marker loci selected from the group consisting of SEQ ID NOs: 57 to 64 and 458 to 468; [0293] any two marker loci selected from the group consisting of SEQ ID NOs: 74 to 82; [0294] any two marker loci selected from the group consisting of SEQ ID NOs: 86 to 89, 476, 477, 479, and 480; or [0295] marker loci SEQ ID NOs: 469 and 470. [0296] 5. The method of any one of embodiments 1-4, wherein said one or more marker loci are within about 5 cM, 1 cM, 0.5 cM, or less than 0.5 cM of any one of marker loci selected from the group consisting of SEQ ID NOs: 1-89 and 446-482. [0297] 6. The method of any one of embodiments 1-5, wherein said one or more NLB resistance QTLs provide mild resistance or resistance to infection by Exserohilum turcicum. [0298] 7. The method of any one of embodiments 1-6, wherein said second population of corn plants or seeds exhibit reduced root rot, reduced stalk rot, fewer leaf lesions, less foliage destruction, or any combination thereof compared to corn plants or seeds lacking said NLB resistance QTL under a high NLB stress condition. [0299] 8. The method of any one of embodiments 1-7, wherein said one or more NLB resistance QTLs confer no yield penalty under a low NLB stress condition. [0300] 9. The method of any one of embodiments 1-8, wherein said step (a) comprises assaying a single nucleotide polymorphism marker. [0301] 10. The method of any one of embodiments 1-9, wherein said step (a) comprises the use of an oligonucleotide probe. [0302] 11. The method of embodiment 10, wherein said oligonucleotide probe is adjacent to a polymorphic nucleotide position in said marker locus. [0303] 12. The method of any one of embodiments 1-11, wherein said step (a) comprises detecting a haplotype. [0304] 13. A method of introgressing an NLB resistance QTL, said method comprising: [0305] a. crossing a first corn plant comprising an NLB resistance QTL with a second corn plant of a different genotype to produce one or more progeny plants or seeds; and [0306] b. selecting a progeny plant or seed comprising an NLB resistance allele of a polymorphic locus linked to said NLB resistance QTL, wherein said polymorphic locus is in a chromosomal segment flanked by: [0307] any two of marker loci SEQ ID NOs: 1 to 18; [0308] any two of marker loci SEQ ID NOs: 19 to 31; [0309] any two of marker loci SEQ ID NOs: 32 to 52 and 471 to 475; [0310] any two of marker loci SEQ ID NOs: 53 to 65 and 446 to 468; [0311] any two of marker loci SEQ ID NOs: 66 to 84; [0312] any two of marker loci SEQ ID NOs: 85 to 89 and 476 to 482; or [0313] marker loci SEQ ID NOs: 469 and 470. [0314] 14. The method of embodiment 13, wherein said polymorphic locus is within about 10 cM, 5 cM, 1 cM, 0.5 cM, or less than 0.5 cM of any one of marker loci selected from the group consisting of SEQ ID NOs: 1-89 and 446-482. [0315] 15. The method of embodiment 13 or 14, further comprising: [0316] c. crossing said progeny plant with itself or said second plant to produce one or more further progeny plants or seeds; and [0317] d. selecting a further progeny plant or seed comprising said NLB resistance allele. [0318] 16. An NLB resistant corn plant, seed, or cell comprising a combination of two or more, three or more, four or more, five or more, six or more, or seven or more introgressed NLB resistance QTLs selected from the group consisting of NLB resistance QTLs NLB_2.01, NLB_3.01, NLB_4.01, NLB_4.02, NLB_5.01, NLB_6.01, NLB_7.01, and NLB_9.01. [0319] 17. The corn plant, seed, or cell of embodiment 16, wherein seed yield of said corn plant is about 1% or more, 3% or more, 5% or more, 10% or more, 20% or more, 30% or more, 40% or more, 50% or more, 60% or more, 70% or more, 80% or more, 90% or more, or 100% or more higher than seed yield of a corn plant without said combination of introgressed NLB resistance QTLs under a high NLB stress condition. [0320] 18. The corn plant, seed, or cell of embodiment 16 or 17, wherein said corn plant, seed, or cell is in an agronomically elite background. [0321] 19. The corn plant, seed, or cell of any one of embodiments 16-18, wherein said corn plant or seed is a transgenic hybrid plant, seed, or cell. [0322] 20. The corn plant, seed, or cell of any one of embodiments 16-19, wherein said combination of introgressed NLB resistance QTLs comprises one or more QTLs selected from the group consisting of NLB resistance QTLs NLB_4.01 and NLB_4.02. [0323] 21. The corn plant, seed, or cell of any one of embodiments 16-19, wherein said combination of introgressed NLB resistance QTLs comprises NLB resistance QTL NLB_4.01 and at least one NLB resistance QTL selected from the group consisting of NLB_2.01, NLB_3.01, NLB_4.02, NLB_5.01, NLB_6.01, NLB_7.01, and NLB_9.01. [0324] 22. The corn plant, seed, or cell of any one of embodiments 16-19, wherein said combination of introgressed NLB resistance QTLs comprises NLB resistance QTL NLB_4.02 and at least one NLB resistance QTL selected from the group consisting of NLB_2.01, NLB_3.01, NLB_4.01, NLB_5.01, NLB_6.01, NLB_7.01, and NLB_9.01. [0325] 23. The corn plant, seed, or cell of any one of embodiments 16-19, wherein said combination of introgressed NLB resistance QTLs comprises NLB resistance QTLs NLB_4.01 and NLB_4.02, and at least one NLB resistance QTL selected from the group consisting of NLB_2.01, NLB_3.01, NLB_5.01, NLB_6.01, NLB_7.01, and NLB_9.01. [0326] 24. A method for selecting a corn plant, seed, or cell, said method comprising: [0327] a. isolating nucleic acids from a corn plant, seed, or cell; [0328] b. analyzing said nucleic acids to detect a polymorphic marker associated with and within 10 cM of an NLB resistance QTL selected from the group consisting of NLB resistance QTLs NLB_2.01, NLB_3.01, NLB_4.01, NLB_4.02, NLB_5.01, NLB_6.01, NLB_7.01, and NLB_9.01; and [0329] c. selecting a corn plant, seed, or cell comprising said NLB resistance QTL. [0330] 25. A method comprising providing a set of corn seeds comprising one or more, two or more, three or more, four or more, five or more, six or more, or seven or more NLB resistance QTLs selected from the group consisting of NLB resistance QTLs NLB_2.01, NLB_3.01, NLB_4.01, NLB_4.02, NLB_5.01, NLB_6.01, NLB_7.01, and NLB_9.01, to a person desirous of planting said set of corn seeds in a field plot. [0331] 26. A method of growing a population of corn plants in a field plot, said method comprising planting a population of corn seeds comprising one or more, two or more, three or more, four or more, five or more, six or more, or seven or more introgressed NLB resistance QTLs selected from the group consisting of NLB resistance QTLs NLB_2.01, NLB_3.01, NLB_4.01, NLB_4.02, NLB_5.01, NLB_6.01, NLB_7.01, and NLB_9.01 in said field plot.
[0332] Having now generally described the invention, the same will be more readily understood through reference to the following examples that are provided by way of illustration, and are not intended to be limiting of the present invention, unless specified.
[0333] Each periodical, patent, and other document or reference cited herein is herein incorporated by reference in its entirety.
EXAMPLES
Example 1. Identification of QTLs Associated with Northern Leaf Blight Resistance in Biparental Mapping Populations
[0334] Biparental mapping populations are constructed to investigate the genetic basis of northern leaf blight (NLB) disease resistance in corn. Plant phenotyping is performed in field plots. About 30-40 plots per mapping population, each plot comprising 10 to 12 individual plants, are inoculated with E. turcicum by placing a sorghum seed carrying E. turcicum spores in the whorl of each plant between V6 (six leaf collars visible) stage and V8 stage. NLB disease resistance is measured 14-21 days after inoculation by rating the percentage of leaf area infected on a scale of 1 (highly resistant) to 9 (susceptible) as shown in Table 1.
TABLE-US-00001 TABLE 1 Description of NLB rating scale, ILA = infected leaf area. Symptoms Score Rating 0% of leaf area infected; no visible lesions 1 Highly Resistant ILA < 1%; few lesions, dispersed through 2 Highly Resistant lower leaves 1% .ltoreq. ILA < 20% 3 Resistant 20% .ltoreq. ILA < 40% 4 Resistant 40% .ltoreq. ILA < 50%; lesions reaching ear leaf, 5 Mildly Resistant with sparse lesions in the leaves above the ear 50% .ltoreq. ILA < 60%; lesions reaching the leaves 6 Mildly above the ear Susceptible 60% .ltoreq. ILA < 75% 7 Susceptible 75% .ltoreq. ILA < 90% 8 Susceptible >90% of foliar area infected, with premature 9 Susceptible death of the plant before forming black layer
[0335] Sixteen mapping populations are shown in Table 2. These populations include twelve NLB resistant parents (CV114258, CV115214, CV099829, CV102084, CV095508, CV103141, CV105893, CV595358, CV593417, CV117407, CV592505, and CV592420). Each mapping population is scored for NLB resistance and individual plant scores from rows of 20 plants each are averaged and reported as a final score for the row.
[0336] Plants from all mapping populations are genotyped using SNP markers that collectively span each chromosome in the maize genome. Marker-trait association studies are performed to identify NLB disease resistance QTLs and their associated markers using composite interval mapping (CIN4) and single marker analysis (SMA).
TABLE-US-00002 TABLE 2 Mapping populations. Map- NLB ping NLB Suscep- Popula- Resistant tible Population tion Cross Line Line Type A CV113018/CV114258 CV114258 CV113018 Double-haploid B CV115214/CV236864 CV115214 CV236864 F.sub.4 C CV108503/CV099829 CV099829 CV108503 Double-haploid D CV102084/CV108986 CV102084 CV108986 Double-haploid E CV102084/CV109562 CV102084 CV109562 Double-haploid F CV102084/CV112706 CV102084 CV112706 Double-haploid G CV095508/CV095869 CV095508 CV095869 Double-haploid H CV095508/CV097202 CV095508 CV097202 F.sub.3 I CV112894/CV103141 CV103141 CV112894 F.sub.3 J CV105893/CV112894 CV105893 CV112894 F.sub.3 K CV595358/CV589205 CV595358 CV589205 Double-haploid L CV582063/CV593417 CV593417 CV582063 Double-haploid M CV625558/CV593417 CV593417 CV625558 Double-haploid O CV603347/CV117407 CV117407 CV603347 Double-haploid P CV592505/CV031573 CV592505 CV031573 Double-haploid Q CV112894/CV592420 CV592420 CV112894 F.sub.3
Example 2. Identification of NLB Disease Resistance QTLs Via Composite Interval Mapping
[0337] A composite interval mapping (CIM) approach is taken to identify NLB resistance QTL intervals based on the phenotyping and genotyping data collected in Example 1. For each marker, the thresholds of likelihood ratio between full and null models for CIM are based on 1000 random permutation tests (Churchill and Doerg, Genetics, 138(3):963-71 (1994)). The composite interval mapping (CIM) analysis reveals several strong QTLs associated with NLB resistance. The QTLs are confirmed in multiple genetic backgrounds and summarized in Table 3.
[0338] In Table 3, genetic positions are represented in cM with position zero being the first (most distal) marker known at the beginning of the chromosome on Monsanto's internal consensus genetic map. Each row of Table 3 provides mapping population ID, number of SNP markers genotyped, resistant parent, chromosome position, the peak of the likelihood ratio corresponding to NLB resistance, left and right flanking positions, p-value, additive effect, and the phenotypic variance (R.sup.2) of individual QTL or Total QTLs.
TABLE-US-00003 TABLE 3 CIM results from all mapping populations. Number of QTL Positions (cM) Mapping Markers Resistant Left Right p- Individual Total population Genotyped Parent Chr Peak Flank Flank value* Additive QTL R.sup.2 R.sup.2 A 112 CV114258 4 168.3 152.3 175.5 0.01 0.53 0.21 0.58 B 90 CV115214 4 155 152 158 0.01 0.7 0.19 0.54 C 158 CV099829 3 113.21 104.2 118.5 0.01 0.41 0.09 0.67 C 158 CV099829 4 166.31 161.3 179.5 0.01 0.36 0.08 0.65 C 158 CV099829 5 80.01 71.9 89.4 0.01 0.44 0.11 0.67 C 158 CV099829 4 72.31 62.8 79.3 0.01 0.86 0.17 0.57 D 169 CV102084 2 107.51 100.5 118.3 0.01 1.04 0.23 0.76 E 173 CV102084 2 115.6 107.5 119.6 0.01 0.51 0.08 0.52 E 173 CV102084 2 114.61 108.6 118.7 0.05 0.64 0.09 0.50 F 171 CV102084 2 113.9 102.9 122.9 0.01 0.78 0.14 0.50 E 173 CV102084 4 174.31 166.3 184.4 0.01 0.47 0.07 0.51 F 171 CV102084 4 172.8 165.8 183.4 0.01 0.51 0.07 0.51 F 171 CV102084 4 172.8 165.8 183.4 0.01 0.81 0.15 0.48 E 173 CV102084 6 74.31 64.3 84.1 0.01 0.65 0.13 0.50 E 173 CV102084 6 78.31 65.3 85.3 0.01 0.93 0.19 0.52 G 111 CV095869 5 93.6 84.9 100.6 0.01 -0.58 0.23 0.49 H 156 CV097202 5 98.1 90.1 108.6 0.01 -0.65 0.2 0.38 I 158 CV103141 9 69.9 60.9 74.9 0.01 0.65 0.18 0.64 J 153 CV105893 5 89.61 85.6 100.4 0.01 0.53 0.17 0.52 K 182 CV595358 5 90.3 86 93.2 0.01 1.43 0.12 0.92 K 182 CV595358 7 109.3 106.1 121.3 0.05 0.92 0.08 0.94 L 173 CV593417 5 90.3 85.1 98.8 0.01 1.97 0.67 0.88 M 174 CV593417 5 90.3 80.6 95.6 0.01 1.7 0.51 0.69 O 142 CV117407 9 69.3 63.6 75.3 0.01 0.41 0.12 0.54 P 150 CV592505 4 165.8 161.2 171.9 0.01 0.45 0.13 0.54 Q 146 CV592420 9 72.9 67.5 83.9 0.01 0.54 0.13 0.53 *p-value is based on 1,000 permutation tests; .sup..dagger. Based on Monsanto's internal consensus genetic map.
Example 3. Identification of Molecular Markers Associated with NLB Disease Resistance Via Single-Marker Analysis (SMA)
[0339] Single-marker analysis (SMA) is performed to identify markers associated with NLB resistance using the genotypic data from Example 1. For each marker, the thresholds (p-value) for SMA are based on 10,000 random permutation tests (Churchill and Doerg, Genetics, 138(3):963-71 (1994)).
[0340] In total, 126 SNP markers are identified to be linked to NLB disease resistance (Table 4). Table 4 also provides the effect estimates on NLB rating score for each marker linked to NLB disease resistance. Further provided are the SEQ ID NO of the marker, chromosome position, marker position on Monsanto's internal consensus genetic map, corresponding marker position on the Neighbors 2008 maize genetic map (publicly available at the MaizeGDB website, maizegdb.org/data_center/map), genetic source of favorable allele, resistant allele SNP, susceptible allele SNP, the estimated effect that the marker polymorphism has on the NLB rating score, and p-value based on 10,000 random permutation tests. For example, SEQ ID NO: 1 is associated with a 0.47% reduction in NLB rating score by one copy of the resistant allele. However, one of skill in the art recognizes that a "resistant" allele at one locus can be a "susceptible" allele in a different genetic background. Thus, this disclosure is not limited to the "resistant" and "susceptible" alleles exemplified herein.
[0341] The primer sequences for amplifying exemplary SNP marker loci linked to NLB disease resistance and the probes used to genotype the corresponding SNP sequences are provided in Table 5. In an illustrative example, SNP marker SEQ ID NO: 1 can be amplified using the primers described in Table 5 as SEQ ID NO: 90 (forward primer) and SEQ ID NO: 179 (reverse primer), and detected with probes indicated as SEQ ID NO: 268 (Probe 1) and SEQ ID NO: 357 (Probe 2).
TABLE-US-00004 TABLE 4 Estimate effects of markers linked to NLB disease resistance from all mapping populations by SMA. MON IBM2008 Genetic Source Exemplary Exemplary Single Permutation SEQ Map Map of Favorable Resistant Susceptible Allele Testing ID NO. Chromosome (cM) (IcM) Allele Allele Allele Effect Probability 1 2 102.8 342.0 CV112894 A G 0.47 0.001 2 2 105.6 347.4 CV102084 G C 0.44 0.001 3 2 106.5 370.8 CV112894 A G 0.47 0.001 4 2 106.7 349.9 CV102084 A G 0.49 0.001 5 2 107.3 351.2 CV102084 T C 0.43 0.001 6 2 108.3 353.5 CV102084 G A 0.60 0.001 7 2 108.3 370.8 CV102084 T G 0.50 0.001 8 2 109.1 355.6 CV102084 A G 0.56 0.001 9 2 109.9 358.3 CV112894 T A 0.54 0.001 10 2 110.0 361.2 CV102084 G A 0.62 0.001 11 2 111.4 370.0 CV102084 A C 0.41 0.001 12 2 113.2 374.0 CV112894 A G 0.56 0.001 13 2 113.6 374.5 CV102084 G A 0.61 0.001 14 2 117.3 380.7 CV102084 G A 0.83 0.001 15 2 117.4 380.8 CV102084 C T 0.37 0.001 16 2 120.2 388.9 CV102084 T C 0.56 0.001 17 2 120.7 390.5 CV102084 A C 0.64 0.001 18 2 120.9 391.2 CV112894 C T 0.66 0.001 19 3 100.8 348.1 CV117407 T C 0.57 0.001 19 3 100.8 348.1 CV099829 T C 0.57 0.001 20 3 102.2 352.2 CV117407 G T 0.55 0.001 20 3 102.2 352.2 CV099829 G T 0.55 0.001 21 3 104.2 358.1 CV117407 G A 0.56 0.001 21 3 104.2 358.1 CV099829 G A 0.56 0.001 22 3 110.9 382.6 CV117407 A G 0.58 0.001 22 3 110.9 382.6 CV099829 A G 0.58 0.001 23 3 111.6 387.5 CV118913 G A 0.59 0.001 23 3 111.6 387.5 CV095508 G A 0.59 0.001 24 3 112.2 390.8 CV117407 T C 0.58 0.001 24 3 112.2 390.8 CV099829 T C 0.58 0.001 25 3 114.9 398.4 CV095508 C T 0.43 0.001 26 3 115.9 401.2 CV118913 T A 0.34 0.001 27 3 115.9 401.2 CV095508 T C 0.43 0.001 28 3 116.5 403.0 CV117407 T C 0.46 0.001 28 3 116.5 403.0 CV099829 T C 0.46 0.001 29 3 118.0 408.4 CV117407 C T 0.44 0.001 29 3 118.0 408.4 CV099829 C T 0.44 0.001 30 3 119.2 412.5 CV117407 G A 0.44 0.001 30 3 119.2 412.5 CV099829 G A 0.44 0.001 31 3 123.4 429.3 CV095508 T C 0.40 0.001 32 4 155.0 531.5 CV095508 A C 0.33 0.001 33 4 162.8 573.4 CV099829 G A 0.30 0.001 34 4 164.3 576.6 CV102084 G A 0.53 0.001 35 4 165.2 578.4 CV102084 G A 0.61 0.001 36 4 165.4 578.8 CV102084 A C 0.60 0.001 37 4 165.8 579.6 CV102084 A T 0.60 0.001 37 4 165.8 579.6 CV102084 A T 0.60 0.001 37 4 165.8 579.6 CV102084 A T 0.60 0.001 38 4 166.3 581.8 CV102084 G A 0.58 0.001 39 4 166.3 581.8 CV102084 T C 0.58 0.001 39 4 166.3 581.8 CV102084 T C 0.58 0.001 39 4 166.3 581.8 CV114258 T C 0.58 0.001 40 4 167.2 583.8 CV095508 C T 0.32 0.001 41 4 168.4 586.4 CV105893 G A 0.34 0.001 42 4 169.3 588.3 CV102084 T A 0.43 0.001 43 4 170.5 590.9 CV102084 T A 0.44 0.001 44 4 171.9 594.0 CV102084 G T 0.64 0.001 45 4 172.8 601.6 CULU085 A G 0.49 0.001 46 4 173.3 601.4 CV102084 T G 0.74 0.001 47 4 175.5 610.2 CV114258 C A 0.59 0.001 48 4 176.4 614.4 CV102084 C T 0.61 0.001 48 4 176.4 614.4 CV102084 C T 0.61 0.001 48 4 176.4 614.4 CV102084 C T 0.61 0.001 48 4 176.4 614.4 CV102084 C T 0.61 0.001 49 4 176.9 616.7 CV105893 A G 0.39 0.001 50 4 183.4 647.2 CV099829 A T 0.34 0.001 50 4 183.4 647.2 CV105893 A T 0.34 0.001 51 4 185.2 655.6 CV102084 A G 0.59 0.001 51 4 185.2 655.6 CV102084 A G 0.59 0.001 52 4 185.7 656.5 CV102084 C T 0.52 0.001 52 4 185.7 656.5 CV102084 C T 0.52 0.001 52 4 185.7 656.5 CV102084 C T 0.52 0.001 53 5 83.7 295.8 CV097202 C A 0.39 0.001 54 5 83.9 296.7 CV095869 T C 0.49 0.001 55 5 86.6 310.8 CV097202 C G 0.47 0.001 55 5 86.6 310.8 CV105893 C G 0.47 0.001 56 5 87.2 313.9 CV097202 C T 0.38 0.001 57 5 89.0 321.3 CV097202 C A 0.40 0.001 58 5 89.7 321.3 CV105893 A G 0.56 0.001 59 5 93.2 325.0 CV105893 T C 0.55 0.001 60 5 93.6 328.5 CV095869 A C 0.58 0.001 61 5 96.7 333.8 CV105893 A G 0.53 0.001 62 5 99.4 336.7 CV105893 T C 0.55 0.001 63 5 102.6 340.0 CV097202 T C 0.41 0.001 64 5 103.3 342.5 CV105893 C T 0.54 0.001 65 5 105.2 347.6 CV095869 G T 0.41 0.001 66 6 63.9 302.0 CV102084 G A 0.53 0.001 67 6 64.1 302.7 CV102084 C T 0.48 0.001 68 6 64.2 303.1 CV102084 T C 0.53 0.001 69 6 64.2 303.1 CV102084 G T 0.50 0.001 70 6 64.5 304.1 CV102084 T G 0.50 0.001 71 6 65.3 306.9 CV102084 T A 0.48 0.001 72 6 66.0 312.3 CV102084 G A 0.45 0.001 73 6 66.4 313.4 CV102084 G A 0.45 0.001 74 6 68.1 318.2 CV102084 T C 0.51 0.001 75 6 68.1 318.2 CV102084 C T 0.52 0.001 76 6 68.3 318.7 CV102084 C G 0.46 0.001 77 6 70.0 324.0 CV102084 G A 0.48 0.001 78 6 71.3 329.9 CV102084 T C 0.46 0.001 79 6 73.2 337.5 CV102084 A T 0.36 0.001 80 6 74.3 341.9 CV102084 A G 0.50 0.001 81 6 74.7 343.2 CV102084 A G 0.26 0.001 82 6 77.7 350.2 CV102084 T C 0.35 0.001 83 6 83.8 369.0 CV102084 C T 0.36 0.001 84 6 85.0 373.8 CV102084 G A 0.31 0.001 85 9 58.1 164.3 CV103141 T C 0.27 0.001 86 9 61.4 188.5 CV103141 C T 0.30 0.001 87 9 63.3 199.4 CV103141 C T 0.46 0.001 88 9 68.6 227.2 CV103141 G C 0.46 0.001 89 9 69.9 240.5 CV103141 A G 0.38 0.001 446 5 80.0 282.6 CV595358 T C 1.64 0.001 447 5 80.0 282.6 CV595358 T G 1.64 0.001 448 5 80.3 283.9 CV595358 C T 1.65 0.001 449 5 80.6 286.7 CV595358 T C 1.65 0.001 450 5 80.6 286.7 CV595358 C T 1.65 0.001 451 5 82.4 291.2 CV595358 G A 1.82 0.001 53 5 83.7 295.8 CV595358 A C 1.66 0.001 452 5 84.0 297.1 CV595358 G A 1.88 0.001 453 5 84.1 297.5 CV595358 A C 1.88 0.001 454 5 85.9 306.9 CV595358 T C 1.88 0.001 455 5 86.0 307.3 CV595358 T C 1.88 0.001 456 5 86.0 307.3 CV595358 C T 1.88 0.001 457 5 88.4 311.8 CV595358 G A 2.00 0.001 57 5 89.0 321.3 CV595358 A C 1.93 0.001 458 5 89.2 310.4 CV595358 G T 1.93 0.001 459 5 90.3 316.9 CV595358 T C 2.00 0.001 460 5 90.3 316.9 CV595358 G C 2.00 0.001 461 5 90.8 320.6 CV595358 G A 2.00 0.001 462 5 91.4 321.9 CV595358 A G 1.93 0.001 463 5 92.0 322.9 CV595358 G C 1.93 0.001 59 5 93.2 327.6 CV595358 C T 1.93 0.001 464 5 93.6 328.5 CV595358 T C 1.87 0.001 465 5 95.3 332.3 CV595358 G T 1.87 0.001 466 5 97.8 335.0 CV595358 C A 1.79 0.001 62 5 99.4 336.7 CV595358 T C 1.79 0.001 467 5 101.8 339.3 CV595358 A G 1.80 0.001 446 5 80.0 282.6 CV593417 T C 1.83 0.001 447 5 80.0 282.6 CV593417 T G 1.83 0.001 448 5 80.3 283.9 CV593417 C T 1.83 0.001 449 5 80.6 286.7 CV593417 T C 1.83 0.001 450 5 80.6 286.7 CV593417 C T 1.83 0.001 451 5 82.4 291.2 CV593417 G A 1.83 0.001 53 5 83.7 295.8 CV593417 A C 1.83 0.001 452 5 84.0 297.1 CV593417 G A 1.87 0.001 453 5 84.1 297.5 CV593417 A C 1.87 0.001 454 5 85.9 306.9 CV593417 T C 1.91 0.001 455 5 86.0 403.0 CV593417 T C 1.91 0.001 456 5 86.0 403.0 CV593417 C T 1.91 0.001 457 5 88.4 311.8 CV593417 G A 1.93 0.001 57 5 89.0 321.3 CV593417 A C 1.91 0.001 458 5 89.2 310.4 CV593417 G T 1.91 0.001 459 5 90.3 316.9 CV593417 T C 1.93 0.001 460 5 90.3 316.9 CV593417 G C 1.93 0.001 461 5 90.8 320.6 CV593417 G A 1.94 0.001 462 5 91.4 321.9 CV593417 A G 1.92 0.001 463 5 92.0 322.9 CV593417 G C 1.92 0.001 464 5 93.6 328.5 CV593417 T C 1.92 0.001 466 5 97.8 335.0 CV593417 C A 1.87 0.001 467 5 101.8 339.3 CV593417 A G 1.72 0.001 468 5 102.3 340.3 CV593417 C A 1.68 0.001 64 5 103.3 342.5 CV593417 T C 1.61 0.001 446 5 80.0 282.6 CV593417 T C 1.61 0.001 448 5 80.3 283.9 CV593417 C T 1.61 0.001 449 5 80.6 286.7 CV593417 T C 1.61 0.001 450 5 80.6 286.7 CV593417 C T 1.61 0.001 451 5 82.4 291.2 CV593417 G A 1.62 0.001 409 5 83.7 295.8 CV593417 A C 1.62 0.001 452 5 84.0 297.1 CV593417 G A 1.70 0.001 453 5 84.1 297.5 CV593417 A C 1.70 0.001 454 5 85.9 306.9 CV593417 T C 1.68 0.001 455 5 86.0 307.3 CV593417 T C 1.68 0.001 456 5 86.0 307.3 CV593417 C T 1.68 0.001 457 5 88.4 311.8 CV593417 G A 1.67 0.001 57 5 89.0 321.3 CV593417 A C 1.67 0.001 458 5 89.2 310.4 CV593417 G T 1.67 0.001 459 5 90.3 316.9 CV593417 T C 1.67 0.001 460 5 90.3 316.9 CV593417 G C 1.67 0.001 462 5 91.4 321.9 CV593417 A G 1.62 0.001 463 5 92.0 322.9 CV593417 G C 1.59 0.001 464 5 93.6 328.5 CV593417 T C 1.45 0.001 466 5 97.8 335.0 CV593417 C A 1.25 0.001 467 5 101.8 339.3 CV593417 A G 1.03 0.001 469 7 103.3 381.2 CV595358 T C 1.03 0.001 470 7 110.0 409.4 CV595358 G A 1.45 0.001 471 4 159.2 564.4 CV592505 G A 0.25 0.001 472 4 161.4 570.3 CV592505 T C 0.28 0.001 473 4 163.7 575.4 CV592505 G C 0.35 0.001 474 4 165.8 579.6 CV592505 G T 0.39 0.001 40 4 167.2 583.8 CV592505 C T 0.40 0.001 475 4 170.1 590.1 CV592505 A G 0.30 0.001 44 4 171.9 594.0 CV592505 T G 0.29 0.001 47 4 175.5 610.2 CV592505 C A 0.13 0.001 476 9 61.6 325.0 CV117407 G A 0.18 0.001 477 9 69.3 232.8 CV117407 C T 0.34 0.001 478 9 79.2 295.7 CV117407 G A 0.22 0.001 479 9 61.5 189.3 CV592420 C G 0.44 0.001 480 9 67.6 340.0 CV592420 C G 0.54 0.001 481 9 70.4 243.6 CV592420 A G 0.50 0.001 482 9 72.9 254.5 CV592420 C T 0.56 0.001 cM = centimorgans, IcM = map units of the IBM2 2008 Neighbors Genetic Map.
In Table 4, "IcM" refers to the map units of the IBM2 2008 Neighbors Genetic Map, which was generated with an intermated recombinant inbred population (syn 4) that resulted in approximately a four-fold increase in the number of meiosies as compared to the typical recombination experiment that is used to generate cM distances (Lee et al., 2002, Plant Mol Biol 48:453 and the Maize Genetics and Genomics Database). "cM" refers to the classical definition of a centimorgan wherein one cM is equal to a 1% chance that a trait at one genetic locus will be separated from a trait at another locus due to crossing over in a single generation (meaning the traits co-segregate 99% of the time during meiosis), and this definition is used herein to delineate map locations pertaining to this invention.
TABLE-US-00005 TABLE 5 Exemplary primers and probes used for genotyping representative SNP markers associated with NLB disease resistance. SEQ ID NO. SEQ SNP Forward Reverse Probe Probe ID NO. Position Primer Primer 1 2 1 101 90 179 268 357 2 101 91 180 269 358 3 101 92 181 270 359 4 77 93 182 271 360 5 101 94 183 272 361 6 136 95 184 273 362 7 104 96 185 274 363 8 112 97 186 275 364 9 902 98 187 276 365 10 101 99 188 277 366 11 205 100 189 278 367 12 245 101 190 279 368 13 43 102 191 280 369 14 144 103 192 281 370 15 101 104 193 282 371 16 247 105 194 283 372 17 341 106 195 284 373 18 91 107 196 285 374 19 216 108 197 286 375 20 81 109 198 287 376 21 194 110 199 288 377 22 46 111 200 289 378 23 859 112 201 290 379 24 200 113 202 291 380 25 73 114 203 292 381 26 352 115 204 293 382 27 162 116 205 294 383 28 106 117 206 295 384 29 319 118 207 296 385 30 127 119 208 297 386 31 101 120 209 298 387 33 319 122 211 300 389 35 101 124 213 302 391 36 373 125 214 303 392 37 115 126 215 304 393 38 171 127 216 305 394 39 37 128 217 306 395 40 101 129 218 307 396 41 101 130 219 308 397 42 101 131 220 309 398 43 101 132 221 310 399 44 2239 133 222 311 400 45 569 134 223 312 401 46 101 135 224 313 402 47 240 136 225 314 403 48 247 137 226 315 404 49 719 138 227 316 405 50 429 139 228 317 406 51 101 140 229 318 407 52 81 141 230 319 408 53 62 142 231 320 409 54 167 143 232 321 410 55 99 144 233 322 411 56 390 145 234 323 412 57 279 146 235 324 413 58 101 147 236 325 414 59 61 148 237 326 415 60 339 149 238 327 416 61 125 150 239 328 417 62 101 151 240 329 418 63 369 152 241 330 419 64 101 153 242 331 420 65 101 154 243 332 421 66 101 155 244 333 422 67 101 156 245 334 423 68 101 157 246 335 424 69 101 158 247 336 425 71 101 160 249 338 427 72 279 161 250 339 428 73 265 162 251 340 429 74 101 163 252 341 430 75 101 164 253 342 431 76 209 165 254 343 432 77 256 166 255 344 433 78 101 167 256 345 434 79 101 168 257 346 435 80 91 169 258 347 436 81 47 170 259 348 437 82 321 171 260 349 438 83 101 172 261 350 439 84 474 173 262 351 440 85 101 174 263 352 441 86 101 175 264 353 442 87 101 176 265 354 443 88 49 177 266 355 444 89 223 178 267 356 445 446 101 483 520 557 594 447 101 484 521 558 595 448 216 485 522 559 596 449 184 486 523 560 597 450 101 487 524 561 598 451 101 488 525 562 599 452 265 489 526 563 600 453 101 490 527 564 601 454 105 491 528 565 602 455 254 492 529 566 603 456 322 493 530 567 604 457 345 494 531 568 605 458 58 495 532 569 606 459 342 496 533 570 607 460 542 497 534 571 608 461 101 498 535 572 609 462 73 499 536 573 610 463 129 500 537 574 611 464 101 501 538 575 612 465 101 502 539 576 613 466 234 503 540 577 614 467 101 504 541 578 615 468 486 505 542 579 616 469 191 506 543 580 617 470 426 507 544 581 618 471 173 508 545 582 619 472 101 509 546 583 620 473 101 510 547 584 621 474 101 511 548 585 622 475 101 512 549 586 623 476 101 513 550 587 624 477 101 514 551 588 625 478 412 515 552 589 626 479 444 516 553 590 627 480 101 517 554 591 628 481 101 518 555 592 629 482 101 519 556 593 630
[0342] One of skill in the art recognizes that sequences to either side of the given primers can be used in place of the given primers, so long as the primers can amplify a region that includes the allele to be detected. The precise probe used for detection can vary, e.g., any probe that can identify the region of a marker amplicon to be detected can be substituted for those probes exemplified herein. Configuration of the amplification primers and detection probes can also be varied. Thus, this disclosure is not limited to the primers, probes, or marker sequences specifically listed in the tables.
[0343] In summary, the QTLs are designated as NLB_2.01, NLB_3.01, NLB_4.01, NLB_4.02, NLB_5.01, NLB_6.01, NLB_7.01, and NLB_9.01 (Table 6).
TABLE-US-00006 TABLE 6 Summary of NLB QTLs. Interval of QTL Peak QTL Chromosome MON Map (cM) IBM2008 Map (IcM) Designation 2 114-117 375-380 NLB_2.01 3 109-114 370-396 NLB_3.01 4 166-169 580-588 NLB_4.01 4 172-177 594-617 NLB_4.02 5 89-98 321-335 NLB_5.01 6 73-75 336-344 NLB_6.01 7 109 405 NLB_7.01 9 63-73 196-255 NLB_9.01 cM = centimorgans; IcM = map units of the IBM2 2008 Neighbors Genetic Map.
Example 4. Validation of NLB QTLs
[0344] Plants with or without resistant NLB QTL are derived. Plants carrying the resistant allele of NLB-2.01, NLB-3.01 or NLB-4.02 show significant reductions in NLB rating score when compared to plants carrying the susceptible allele (Table 7).
TABLE-US-00007 TABLE 7 Validation of NLB QTLs. NLB QTL Resistance Infection p- interval QTL Profile Score value NLB_2.01 Absent 5.88 2.74E-58 Present 2.47 NLB_3.01 Absent 5.3 1.70E-37 Present 3.5 NLB_4.02 Absent 5.34 7.23E-27 Present 2.92 NLB_6.01 Absent 3.78 0.15 Present 4.26
Example 5: Introgression of NLB Resistance QTLs into Additional Maize Lines
[0345] A maize plant comprising one or more, two or more, or three or more NLB resistance QTLs is crossed with an elite maize line comprising a desirable trait (e.g., improved yield under water, temperature, or pest stress conditions), but susceptible to NLB. F.sub.1 progeny plants from this cross are assayed for one or more SNP markers exemplified in Tables 4 and 5 or molecular markers linked to those SNP markers to select for NLB resistance QTLs. A selected F.sub.1 progeny plant is then backcrossed with the parent elite maize line comprising the desirable trait (recurrent parent). Plants from the BC1 generation are also genotyped using SNP markers exemplified in Table 5, or a linked marker, to select for NLB resistance QTLs. After multiple rounds of backcrossing (e.g., 5-7 generations) with the recurrent parent line, a new elite maize line is obtained comprising both NLB resistance and the desirable trait in the recurrent parent line. Using the above introgression and marker-assisted selection strategy, the pyramiding or stacking of multiple NLB resistance QTLs can be achieved.
[0346] As various modifications could be made in the constructions and methods herein described and illustrated without departing from the scope of this disclosure, it is intended that the foregoing description shall be interpreted as illustrative rather than limiting. The breadth and scope of the present disclosure should not be limited by any of the above-described exemplary embodiments, but should be defined only in accordance with the following claims appended hereto and their equivalents. All patent and non-patent documents cited in this specification are incorporated herein by reference in their entireties.
Sequence CWU 1
1
6301201DNAZea maysmisc_feature(101)..(101)n is a, c, g, or t
1agccttatgt ctgatggcct ccgctaaacc gtcagacata agggacatgg gccctgccaa
60cagttgttaa gaccgttatc gaggataaac gtcgacaacc naaaggttcc atcggacata
120gcttatttcc gatggccgcc gttggaaata aggttatttt tgacccttta
cggccgaggg 180acaagtaccg tcggacataa g 2012201DNAZea
maysmisc_feature(23)..(23)n is a, c, g, or
tmisc_feature(101)..(101)n is a, c, g, or t 2atacatgggc atcaacgagt
ccnaaaaaaa caagagcgga aaaaaaacaa gagcggaaac 60tggaaatgag tccgtgtgtt
atttatgacg cccgcaaggc ngcaacgcca gtttggtgga 120agccccagta
ccgtctgact ttgctggact gtacgtaatg cacgtaccat cctcctaatc
180ccatcctaac ccccgcaatt a 2013201DNAZea
maysmisc_feature(10)..(10)n is a, c, g, or tmisc_feature(29)..(29)n
is a, c, g, or tmisc_feature(75)..(75)n is a, c, g, or
tmisc_feature(101)..(101)n is a, c, g, or t 3tgctattcan accattaaat
ttcgaggcna aattcagggt cgaaaagttt tggttcttgt 60ggattccgat agttntcatt
catttctcag tttgcgggtg ncgtctaaat tagagggtgt 120gtcagagatg
ccatggccag tgatggtgca agtagctgat ggtggtagat tgttatgtga
180caaacagttt ttgggggcca c 20141186DNAZea
maysmisc_feature(77)..(77)n is a, c, g, or
tmisc_feature(1164)..(1165)n is a, c, g, or
tmisc_feature(1172)..(1176)n is a, c, g, or t 4tggcgtgatt
ctaggtaaaa gtttatgttt acataataat cagagatgta tttcgagtaa 60ctgaattatt
gtatatncct tcgtcctttt cctttcttgg aaactagctt tgtaaactat
120tgctttacta tactaagttg ggaaacctac ttctgttagt agtctccttt
tgcaagtgtt 180tttctggtta atctttcata acgagactgg agtgaatagg
ttaaatggat tatgatccct 240atcgcatgca gccttttttg tatgcacaac
agcatttttt tgttgctgca agccttctac 300ctttgagaat atatttgctc
ttacgtagtt atgtattttg acaacatagt tttggtcatt 360gtttagtact
cctataagct cccgcgtatc ctatcaggtg catttccttt tagcttataa
420aattttccct taatccttta tatatgctga ttttacatgt tccatcccat
tgggaattaa 480aactgtaata cctaaaatat atgttagttt acttctcact
gtttcatgag gatgtctttt 540caagttttca gcaaatttgg tgatatcttt
gacgaagatc acttcattga gtcacttaga 600aaatatgtaa gggttgtaaa
agatcttcct gaagatgttt ttctgcgatt caaccataat 660atcagcataa
taccaaacat gagaaccaaa gctttctcac ccccaagtta ctacttacaa
720catgtgcttc caaagctgtt ggagctaggg tacgtgctcc ctcttttttc
tccttgacat 780gaagtagagt ctgatatttg cactgagcat cacatagcac
catgtcttca cttatttctt 840gttctatgac acagggctgt gcgtattgcc
cctttctcaa atagactggc tcattcagtt 900cctatgaata tccaggcatt
gagatgtttg acaaactata aggcattaag attttctgaa 960ccaataagaa
ttcttgcaga taatatggtt gaccgaatga tcaagaggag ttttttaagt
1020ggcgggaagt acgtctcggt tcatcttcgc tttgaagagg ttttactaaa
gtttatcttg 1080tgtggactaa tattactact tttctcaagt tgtgatgagt
tatatgtcta ggacatggta 1140gctttttcat gctgtaatta tganngtggc
tnnnnngaga ataatg 11865201DNAZea maysmisc_feature(46)..(46)n is a,
c, g, or tmisc_feature(101)..(101)n is a, c, g, or t 5tttggccatg
tcagggaaaa aaaggtagga tgaattgaat gcattnttgc cagtttcacg 60caacactaga
taagcaactg caatgagaga ttataagaag naacaagctc acttttgtat
120ccgtcttcag cctttttgct ggttcattgt cgcagcagtt ctcgggactg
ccatctgaat 180ctggcttggt tattgaaatc g 2016355DNAZea
maysmisc_feature(31)..(32)n is a, c, g, or
tmisc_feature(136)..(136)n is a, c, g, or t 6tgatggcggg ggatcagggt
ctgggtccgg nnccggatcc ggtgatagta gtccttatgg 60tacccatgca agtgctggag
ggggtggtgc aggtggtgga gctagccaat acggtgggtc 120tggatatggt
tcaggntcag ggtctggttc agggtctagt acatatagtc aaggagggta
180ttattcgggt tatggagaat cttctaatgc tggtggttct ggtggaggtg
ggggtggagg 240acaagctgga ggttatggga attccaatgc tcaaggatct
ggtagtggca ccggttctgg 300ctctagctat gctaacaggt attgggatgg
atcaagtgga ggtgcaaatg ctaat 3557355DNAZea
maysmisc_feature(104)..(104)n is a, c, g, or t 7aagagaacgt
gactcctgcc tctaaggtga agaccgttgt taggggagtt cttggtacaa 60ggatggataa
ttcagtgtca gcagcaaatg cttcaaataa gaanaaagtt cttgggtcaa
120gggtagataa ttcagtgtca acagagaata gctcaaataa gaagcaatgt
gaattatcat 180cgaagtccaa aaaagtccac acagtagatt ttgatgtttt
ctattcggat aaggagaact 240tgactcctat atcttcagga ggcatgaaag
caaggaagtg ttttcccaag gacctctcag 300tcgacttaga ccaagatctg
gaagcattct gctcagacaa ggagaacttg acacc 3558273DNAZea
maysmisc_feature(112)..(112)n is a, c, g, or t 8aaaaggaacc
attgcatgca ggagaaaaga aatgatgaga taaagggaaa aaaaaggagg 60gagagagaga
gaggaaccaa tgcaagaagg tgatccgatc aacaggcaga gncatggcca
120ttggtcgatc tagccacggt ctctactcgc tgccgggggg cttgtaggcg
atgaagctga 180cgcactgcgt ctgcctgacg ttgtcgaagc cgatgacgcg
gtggaaggcg tccgggtacg 240cggcgatggc ctcctgcagc tccttgtacc acc
27391024DNAZea maysmisc_feature(902)..(902)n is a, c, g, or t
9ctctaggtct gttttctttt ggcttcttgt acgcagttct atcacctaag ttttattttc
60ctatgttact ggacatattt tctttatttt atgtcttatt actaatcttt acctttggtt
120ctccagccta gcagttattt ttggtgctgg acttgtaact agtttatctc
cttgtacact 180aagtgttcta ccactaactc taggatatat aggtaactat
attgttttgt ttgagatgat 240tgttggcaaa aatgcccaga attgatcatc
attaattact ctgattcagt gctttcttca 300taaacattta ggtgcatttg
gctcagggaa aggccgatcc gaggtaattt aagatgagtg 360aaaaattgta
cttctttatg ccatggaaaa acttgaagat attgctattt atctgttagg
420atatgtatta ccacaaagtt atcttcactg aaggatttac ttgactaatt
aaggctatga 480ataatgttga aacctgatta ttctagctgg gggcttccct
tgatacatgc attgacttgg 540cttttctatc aattataggt tgttgggaat
tcaattgcat tttcactagg actagcaaca 600accttagcca ttcttggtgt
tgctgcttct tttgctggaa aggcttatgg tcaggtagga 660caagggctcc
cagtggctgc ttccggtttg gccattatca tgggattgaa ccttctggag
720gtaaatcaca tgaaacagaa actacatata tagactgaag catgccaatc
agaatttcct 780cctaccttgt ttccttaagt tacaagatga ttcaatccct
gtatttcttg gtttctcatt 840ataaatacta ctgtagaagt acaaaccaat
tttgtgatag tttgttactt tgttcttatg 900antttcgtat tcctgcttgc
tcctgtaaag tacatgtaac tacgttatca aatgttctgc 960cctaagctgt
ttattatctg tccttaacag gtacttgaat tgcaacttcc ctcattttca 1020gcga
102410201DNAZea maysmisc_feature(23)..(23)n is a, c, g, or
tmisc_feature(101)..(101)n is a, c, g, or t 10gtaaaggcct catagtgtta
ccntgcctcg cttccttggt agaggtgtat gggggtcgta 60caaccgcatg gcagaaacaa
atacatagaa gattactaaa nagatactgg aaggcttatc 120tgaagatact
ggtgacagcg atagctatga tgtggaaagc ggggacgaag attttgagga
180tcggccctgg aggccaagtc a 20111460DNAZea
maysmisc_feature(205)..(205)n is a, c, g, or t 11aacagcagca
tatgccatat cagccatcca accgctctcg gttcagcctt tcatttcatt 60tctagggtta
atcccgaacc cgccagtttc agcagggctc gctcagtcga aggatacata
120agaccacagt gaaaattaga gggccttaca aaagcagcag cagcagcaat
gcagcacgca 180gatcaaattt acacatcaaa acgcnggcca aaacccgaga
agaaaccgaa accccgccgc 240cgccaagcac acacatgcac ccacacacac
acaccaccgc ccacggccgc aatatacaca 300acggaaccta ccccggcaac
tccaaccgaa acacagcacc catcaccgca ctcggcaagc 360cgtggccggg
acggcagggg agcacttggg gttcgggatg gccctgaagt ggacgctgtt
420cccggttatc ctgcggcgac ccctatagtc aggaaggcca 46012381DNAZea
maysmisc_feature(245)..(245)n is a, c, g, or t 12agctgacaca
gatatcgagg accaggagaa caatgcaatt ggtgatgatt acaatgatga 60ccttatggat
gaagaagatg acgactttct tgagaaccat gttatagaag taagatggag
120ggagagctta actcgaatgg attatgatca tcatctgaga ttttccaggg
gtcgtgcaga 180ctccagtggt tttattgata tatcttctga gtcatttcat
ggtgtgggga cagttgattc 240atttnatctg catcgttcgt ttggtcttga
acggcggcgc caaatcggaa gtaggtcgct 300tcttgatcga ccaaggaccg
atgggaatgc ctttcttcat ccactgcttg tcaggccagc 360acagtctagg
gagggaacta a 38113484DNAZea maysmisc_feature(43)..(43)n is a, c, g,
or tmisc_feature(140)..(140)n is a, c, g, or
tmisc_feature(144)..(144)n is a, c, g, or
tmisc_feature(158)..(158)n is a, c, g, or
tmisc_feature(280)..(280)n is a, c, g, or
tmisc_feature(285)..(285)n is a, c, g, or t 13ttgcctcttg tacaaatctt
gtgactcgtc ctgcatcgaa ggncaacaca atttgagatc 60tggactcaag gtgtgcctgg
aaacgcttta cagaaacatt gagtgaaatg catgcataca 120aggcctgaga
actgactgcn aagnggggaa aaacactnta cagaaacatt gagtgaaaac
180aatattaatc gctctcttag ccacaacagg ctaaagctaa gctaagctaa
gccaatggca 240ctcagagatt cggtacggca ttttcttctc tggctttttn
aaaanggact acttgcaagt 300aaatgacaat gtgtatacct tctctataga
accaaataat gctcccacaa gtcacggaca 360aagaggtgat ataatcgagc
atgacaatgt atcttaacca ttcggtatca tagtactata 420gcatccttcg
agacaaattt acccatgaac cttggtatgc ccaatttaca tgcacaaaga 480gaca
48414648DNAZea maysmisc_feature(144)..(144)n is a, c, g, or
tmisc_feature(203)..(203)n is a, c, g, or t 14gatgggcatt taccgagtgg
aacataaccc attgtgacat aaaaaaagaa aaggttagac 60tctttgctcc cagtaagaaa
acatcagtag ctccaattac aacactttta gcagggtatg 120ttacgcagtg
agggattggg aggnttctaa tgtcaactag tgtggaacag tagcgcatat
180gaactttcca actgaatcct ttnaaatcct agttgctata acacatttag
caaacttatg 240tgaatcctat gagaccctca catcctgtat tattctttct
gcattagcct ctccacatac 300ccactagcta tcactacttc ctgaacacag
aactatctgg ctccactact attggacata 360catcagttac tacctagaat
agctagctac actactatat tttcaacaag agtcttatat 420tcatcctagt
atctatctct ctaattactc accccatcac tgttacattt aatcacaatc
480ttatccttcc cacctcatat caatatgctt tttcacatac ttacctattt
ctattacata 540tcaatcgtac tattcttact tcagtctatc acacctcttt
accaatcatg tataacttaa 600tatacctatc tactacttat ttactatatc
tatttcttca tacttatt 64815201DNAZea maysmisc_feature(44)..(44)n is
a, c, g, or tmisc_feature(75)..(75)n is a, c, g, or
tmisc_feature(79)..(79)n is a, c, g, or tmisc_feature(101)..(101)n
is a, c, g, or tmisc_feature(152)..(152)n is a, c, g, or
tmisc_feature(165)..(165)n is a, c, g, or
tmisc_feature(168)..(168)n is a, c, g, or t 15tccgtgtcca tttcttacag
tcttccaact ctccttctca actngtatat acaagcactc 60actagattgc tattnaagnt
gaacaccttc tcaacgagtg nactgtgtac tatgtgatac 120tagtcctact
catacttaac acttgttatg gncttttaat ttttnttnat taattgctag
180actagttata ggctaagcca a 20116677DNAZea
maysmisc_feature(247)..(247)n is a, c, g, or t 16cgtgcccgtg
cgtttccttg tcgcatcgca cgtagccatt aattcccatg cacgtaggcc 60accgctgcat
gctgcacggc cacgagaaca gcacacgccc ctcgttacgt tcctggcatg
120catatctgat tgcgtttctt gctccttgcg tgttcctcgt gcatcgtggc
atgtcgatcg 180gcggatcgag caggtgtggg aggtgccgga gggcgcggag
gttctggcct cctccgacaa 240gaccggngtc gagatgttct gcgtcggcga
gcacgtgctg ggcatccagg ggcacccgga 300gtacaccaag gacatcttcc
tcagcctcgt cgaccgcctc ctcgccgcgg gatccatcac 360cgtgagtgcc
tgctttcacg tcaccttaca gcgactgatt cctctcacca aacggacaag
420cagccgcgtc gctctccgca tctctactct gattccgctt tgccactcgc
acacgctgca 480gattcccttt gctgaggccg tgaacaggca gctggagacc
actgcgccgg accgggagtt 540ctggctcatg ctctgcaaga gcttcctcaa
ggctcgtgaa gaataagagt attaattaga 600gtttgcacac tgtgatgtca
ttgtcactta ccattaaaac ggttgtaaat cttctatcac 660ttcaacaaat aaacctc
67717428DNAZea maysmisc_feature(341)..(341)n is a, c, g, or t
17tgctaatcct tgtcgtcccc ttcctcgccg catcgacgtc ggcggacggg aaggccggag
60acatgcagcg gcggcggcgg cgacaggtcc tgctgcggga caaggcgact ctgctggacc
120tgaagcaggg gctcaggctg tcatcttcag cggccctggc ggactggaac
gagtccaacg 180gcgacgtctg cggcttcacc ggcgtcacct gcgactggcg
gcgggagcac gttgttgggc 240tctctctcgc caacttgggc atcagtgttg
ctattccacg ggtcattggc aatctctcgc 300acctccgggg cctcgacgtg
tccaacaaca caatctctgg ncagataccg acgtccgtcg 360gcaacctcac
gcgactggag cgcctcttca tgaacaacaa cgacatctcg ggcaccatct 420cttcaatc
42818452DNAZea maysmisc_feature(91)..(91)n is a, c, g, or t
18agagtgctat ataccattcc ggattgacgc ctcggctgat ggcggggagg aaaacaaatg
60cttgaaaggg ggtgtttact tagctgacgg ngacgaaact gttccagttc ttagcgcggg
120ctacatgtgt gcaaaagggt ggcgtggcaa aactcgtttc aaccctgccg
gcagcaagac 180ttacgtgaga gagtacagcc attcaccacc ctcaactctc
ctggaaggca ggggcactca 240gagcggtgca catgttgata taatggggaa
cttcgctttg atcgaggaca tcatcaggat 300agctgccggg gcaaccggtg
aggaaattgg tggcgaccag gtttattcag atatattcaa 360atggtcagag
aaaatcaaat tgaaattgta acccatggga agttaaaaga agtgccccaa
420cccgttcatt gcgttcctaa atgcttgcct ga 45219393DNAZea
maysmisc_feature(216)..(216)n is a, c, g, or t 19gcggcggcgg
tggaggtggc tgaccatagc gccttcgttg gagcagttgt tgggaaggag 60gtggaggtgc
aggtgcaggt gcaggtgaaa gaagaggagg agcaggagga ggaggaggag
120gaaggaaacg aggaggagct gcacacgaga gtggaggact tcattgcgag
ggtcaagagg 180caaaggaagc tggagctcaa gagcttcttc gatgtngatc
gatgatgact atatgatatg 240tttggcaggt gcaaaattac agtaaggttg
cgggcgccct aaaaaggatc tgtaatggtt 300tgtgctgcta gctatccttt
tgattaattc tgtcacttgt agttgtagta ggttaaaatg 360tcgaaggtaa
aacgataagg agacgaaccc atc 39320686DNAZea
maysmisc_feature(81)..(81)n is a, c, g, or
tmisc_feature(252)..(252)n is a, c, g, or
tmisc_feature(389)..(389)n is a, c, g, or
tmisc_feature(485)..(485)n is a, c, g, or
tmisc_feature(570)..(570)n is a, c, g, or
tmisc_feature(644)..(645)n is a, c, g, or
tmisc_feature(650)..(651)n is a, c, g, or
tmisc_feature(657)..(663)n is a, c, g, or
tmisc_feature(668)..(668)n is a, c, g, or
tmisc_feature(670)..(670)n is a, c, g, or
tmisc_feature(673)..(683)n is a, c, g, or t 20ttcccattcc catcaagcaa
gcacattctc acttttccag catcaaggca tggagtatgg 60caacggatga caccttgctg
nataatttcc acacatacct taacatcacc aaacagcact 120tgccatgaac
tgtgaggggg attgcaaagg aagtctccta caatgataac ctgcattaca
180ttttcataag tccccaacca ttgtagttct cggcacaggt ttgtccaaac
tgataagttc 240ttttttgttt cnttgggttc tctggactca ggcttcaagc
tttcagatca aaatacttaa 300gtattactcc tcaattctga tttctaaaag
taacttgatt ttagattttg ccactcgtat 360atatatgcag gtcattatag
gattctagna acccgtcccc cattttttct tcttatgccc 420atgctaaaca
atctactaat cacagttaaa ggtcatttga atcatttcaa cacttcatta
480atttntatgc acaagcctaa aacaacttac atttggcatc agagtatgta
agtaccacat 540gaagatatgt tttgttcaat attatgccan tagaaaagaa
gaaagaagaa tgcatgttgc 600agtaatttaa tcaagcctag taactcgtac
tcatatcata cctnngtacn ntcatannnn 660nnngctgntn ctnnnnnnnn nnnatg
68621417DNAZea maysmisc_feature(194)..(194)n is a, c, g, or t
21tgatgtttga cgggcaggac gacaagctgt tcgagcactt ctccatggtc gcgcagaggc
60ttggcgttta caccgccagg gactacgccg acatcctcga gttcctcgtc gacaggtgga
120aggtggcgaa cctgactggt ctgtcgggtg aagggaacaa ggcgcaggac
tacctttgca 180cccttgcttc aagnatcagg aggctggagg agagggccca
gagcagagcc aagaaagcag 240gcacgctgcc tttcagctgg gtatacggta
gggacgtcca actgtgagat cggaaacctg 300ctgcggactg cttagacaag
acctgctgtg ctgtgtctgc gttacatagt tctccaggtt 360ttgatcagat
ggtcccgtgt cgtcttatag agcgatagga gaacgtgttg gtctgtg 41722254DNAZea
maysmisc_feature(46)..(46)n is a, c, g, or t 22agggtagtat
atgtgcattc atcgtttttc attagccttg attagnccaa agtgatagtt 60tatgcttggt
catcgagagt ttggtgatca gacgatgaag attgtgagtg gcacaactta
120agaggtaaac agttgtgtga ttcaacatag tagagtgaca aatgatcgac
tcatagagag 180ccctcgtatg agacgtgagc gacactcctt cataggtgtt
ctaataagga ttagttagaa 240gtgtcaactc ttga 254231041DNAZea
maysmisc_feature(859)..(859)n is a, c, g, or
tmisc_feature(985)..(993)n is a, c, g, or
tmisc_feature(1017)..(1021)n is a, c, g, or t 23ctcccttcgt
cgcgctggtc gctgccgcct ccccctcgtg ttcacactcc tctaggcgcc 60tcaggcctca
cggtcttcgt cctcgcctcg gcgtctcagc ctcgcgaccc gcgtggagct
120cacggcggcg accgccattg tagttcccgg aggtacaaag ctcgcctctc
tctcctcgcc 180tggaatgcct agctagcttt cttcgcttct agcgtttctc
ggctcccgtg tattgtaatc 240attatcacgc gcgtttgatt gctttgggct
ttggcatcgt cccgtgcagt tccttcagca 300tttgtgattt aggtgcttgt
tcaagtgctg cgattgggtg ggagcctgat ttggtgcgca 360aggtgcataa
aactcgcaca cgtgcacgca cggcgcgctc tgaggttggg atcgggagta
420gcggcaggtg tttcaggtga tcctgtgttt ccgctcggct agaggtcctg
tgattacgac 480agatttaggg cttagggggg aattggattt agtgagctta
ctatgggaag agtactcttt 540gggctaagtg gagtttctga tcgtcagctt
ttgcatgcct tgagtatgat aattcgttgt 600tttcatgcct aagtttctac
atttcaagag ataggaacga aagtactccg tatgcgcgat 660ttaaggtcaa
atacattcga caggctatac tggcgatcac tttatgggat gtgcttttat
720atgggactgt ctatgtttcc ttaagtggag taagacccat aaatggcgtc
tgaaatttct 780ttaagctgta ccactttttt tatggtttgg taagttctgc
agctaaaata gagtcttcaa 840tcttgataca ggtcaatgna ggagtcagtg
ccaatgtcag taatcagcag tatttcaaac 900ttccgcacgc tgtctaccag
cagtgtggta gaaactgagc tagttaagag atactgccgg 960aagatcaatg
aaatcctggg tcttnnnnnn nnngtcctgg atgaagttct cacccannnn
1020nagtctggat gataggatgc t 104124748DNAZea
maysmisc_feature(42)..(45)n is a, c, g, or tmisc_feature(64)..(64)n
is a, c, g, or tmisc_feature(91)..(91)n is a, c, g, or
tmisc_feature(119)..(119)n is a, c, g, or
tmisc_feature(132)..(132)n is a, c, g, or
tmisc_feature(200)..(200)n is a, c, g, or
tmisc_feature(272)..(272)n is a, c, g, or
tmisc_feature(295)..(295)n is a, c, g, or
tmisc_feature(350)..(350)n is a, c, g, or
tmisc_feature(522)..(522)n is a, c, g, or
tmisc_feature(536)..(536)n is a, c, g, or
tmisc_feature(614)..(615)n is a, c, g, or
tmisc_feature(635)..(642)n is a, c, g, or
tmisc_feature(647)..(647)n is a, c, g, or
tmisc_feature(649)..(651)n is a, c, g, or
tmisc_feature(654)..(656)n is a, c, g, or
tmisc_feature(666)..(667)n is a, c, g, or
tmisc_feature(673)..(679)n is a, c, g, or
tmisc_feature(685)..(693)n is a, c, g, or
tmisc_feature(706)..(706)n is a, c, g, or
tmisc_feature(711)..(711)n is a, c, g, or
tmisc_feature(715)..(723)n is a, c, g, or
tmisc_feature(726)..(730)n is a, c, g, or
tmisc_feature(732)..(742)n is a, c, g, or
tmisc_feature(744)..(744)n is a, c, g, or t 24cataatgcaa caaacgttaa
aaaactacag agatggaaaa annnncagtc accataaaag 60atgncttgta
caacaagttc
atgcaaaaaa nttattatta ctattaatga gctaactana 120aacaaacacc
cnaaatataa catcatgaca tcaaaaggaa gacatgtacc ttgagggcac
180caaagacacg gttcccagtn gtagtcctaa taaggccaac atccaagaga
gcacggaaag 240gcctcctctc atcagctggc tcaacagaga antcctcacc
agtggcctga tgaangaaaa 300gaaacaatat aaatgcccaa ccaatgtgac
atcagcaaaa aataagatcn aaatttagcc 360aaattttacc tcaacattgc
cctcatattc cttatctaaa ccacgggtct tgagcacacg 420gcgagccaac
agaaggccag tgcagtaggc tacaagatga gtgtaagcag ttgaactagt
480gaatatataa attcattaag gtacaatatg caatttacac antatttcca
gaaggnaaat 540taccagctgc atagttggtc agaccaactt caagaccata
tcgtggcaac tcatgcgagt 600aagcagaagc aagnnccata tcacctgcta
tactnnnnnn nntgatntnn nctnnnatgt 660ccttgnnggt ctnnnnnnnt
ctagnnnnnn nnnacaatat tatgcnactg nagcnnnnnn 720nnnatnnnnn
tnnnnnnnnn nnanatga 74825714DNAZea maysmisc_feature(35)..(41)n is
a, c, g, or tmisc_feature(73)..(73)n is a, c, g, or
tmisc_feature(279)..(279)n is a, c, g, or
tmisc_feature(651)..(652)n is a, c, g, or
tmisc_feature(668)..(669)n is a, c, g, or
tmisc_feature(673)..(673)n is a, c, g, or
tmisc_feature(693)..(693)n is a, c, g, or
tmisc_feature(695)..(696)n is a, c, g, or
tmisc_feature(702)..(709)n is a, c, g, or t 25ctcagcagtt ttaagtttct
ctatagttca cactnnnnnn nacaaatccg gctgtgccca 60aacaactcta ttnaacagag
aagcatcgaa actagcatgg agcaaaatct gaattcctta 120aatctccaac
ctaaaacact tagccgacca aaaagcccct gcttcccaaa cgattcgcca
180agttcgttaa agattttggg ggaaaacgag gcctaaacaa cgggaagtaa
gggaaatcaa 240tagaaaaaac atggttttgg tgagaacctg tgcctagcng
cgctctgggg aagattggct 300gagggagctg cgttttgggg gagtgcaaga
ggagcacggt atccagggaa gatgtgcggg 360agagagatat atattggata
cagctcaaga ggggaggaac gctcctcctt aattgttcaa 420ggcaacgctc
ctgccttctt ccaaaaaaaa gaggggagga tcctgacaaa attccagcaa
480agataggtgt gagagtttat tgctgccttc cttttctgct tgcctttttt
atctcctcaa 540aagagttgtg tttgtgttct atcccccgta aaacacaatt
tggaaagtgt ttctcaaaaa 600ttctgatgca tgcattgact ctcttttttt
tgtcactatc ctggatttct nngtattctt 660atttccanng tantgataac
taaaaaaatg aancnntagt tnnnnnnnnt atat 71426410DNAZea
maysmisc_feature(352)..(352)n is a, c, g, or t 26tcgttaacaa
ggagaggaga gaacgggttt cgtggaccgg gccggtcttc cactatgggc 60ttgttaggac
atacaaagac acagccgaaa tttgctgttg gtctcgagca aggccacggc
120ccacaggtct gcttgggttt gggtctagga aaggaaaaga actactctca
ctaaaatgag 180agaaaggtgc ggtaaaaaat gagagaaagg tgcggtaaaa
aaaaccaaaa agagagaaag 240atgaaaaaga agacttcaaa cgcactggca
tagacgccac ggcagtccag tggcaaccgc 300atcagtgcgt atttacggag
caaaacagat ctccccgtcg acctggtaca cngccgcagt 360tggtatcagg
acgtcgcacg gcaggtcacg agtcttcaga gttcagagag 41027359DNAZea
maysmisc_feature(162)..(162)n is a, c, g, or t 27gcagctcaaa
gcattcgtca agcaggatca acaagggacc ttgttattct tgttgatgac 60accattagtg
accaccaccg caaggggctg gaatctgctg ggtggaaggt tagaataata
120cagaggatcc ggaatcccaa agcggaacgt gatgcctaca angaatggaa
ctacagcaaa 180ttccggctgt ggcagcttac agattacgac aaggtcattt
tcattgatgc tgatctgctc 240atcctgagga acattgattt cttgtttgca
atgccggaaa tcaccgcaac tgggaacaat 300gctacactct tcaactctgg
ggtgatggtc attgagcctt caaactgcac gttccagtt 35928384DNAZea
maysmisc_feature(106)..(106)n is a, c, g, or t 28atcattcctt
tatctattgg aaatctcaaa gggttaaatg tctttgatgc acatcattgc 60aacttggggg
ggccaattcc agcaagcata ggcaacatgt cgaatntgtt gacacttgat
120ttatcaaaga actccctcga tggttcaatt tccaatgaga ttttcaaact
gtcatccctt 180gacccttatc tatttaaagt tttaaactta tcatacaatt
cgctatcggg acatcttcct 240tctgagatga gtagtttggg gaacctgaac
caactagttc tgtctgggaa ccgattgtct 300ggcgagatac ctgagagtat
tggggaatgc actgtgctgc aataccttat attggatata 360taactcaatc
gatggaagca tacc 38429788DNAZea maysmisc_feature(319)..(319)n is a,
c, g, or tmisc_feature(721)..(721)n is a, c, g, or
tmisc_feature(736)..(741)n is a, c, g, or
tmisc_feature(746)..(757)n is a, c, g, or
tmisc_feature(768)..(773)n is a, c, g, or t 29gtttgcttcg cagctgtttg
taggacatgg agagaaatgt gtaaagagat tgtgttgagc 60ccggagtttt gtggcaagct
caccttccct gtgtctctaa aacaggtaag ggaaagaaca 120atcaacctcc
ttcagttcga gcaatcttga accggatgtt gagaattgcc tccaaacctt
180gagcagcgga tatctatatc tccctctggt ccttgatata ttgcttgttt
cttttgacag 240cctggtcctc gagatggaaa tacaatggtc cagtgtttta
taaagaggaa taagtcaaaa 300tccacttacc atctctacnt gtgccttagc
aatggtacgt cacatgattt gcacattttc 360aagattcaat agcaatgttc
ataagtttat actgttttat tggagcatat gggattattg 420ttagccagat
atgtttctcc tttttgagtg tttgattttg tgattatatc tcgtatcttg
480taatcctcat aaattctgaa attgtgtggc cttttaatct catattcatg
agagtattta 540cagttaaatg atcaatggtc tgtttatctt gtttcacttt
atcaaatttg ttgactcatt 600ttcttctgtg cacagttgtt acttcagaaa
gtgggaaatt cctcttatca gctaaacgac 660accgcaaaac cacatgcacc
gagtacacta tatcaatgga ttctggcaac atctcaagat 720ngaaaagaac
ctacannnnn naaatnnnnn nnnnnnnatg tttttgannn nnntgttctt 780gtttcccc
78830443DNAZea maysmisc_feature(33)..(33)n is a, c, g, or
tmisc_feature(49)..(49)n is a, c, g, or tmisc_feature(127)..(127)n
is a, c, g, or tmisc_feature(199)..(199)n is a, c, g, or t
30actataatct acaatgaata gcaagcaatc ggntattagc ttctatacna ttgactacca
60tcaatccttt gtaagtgccc ctgacaactc tttgtaccct tgacagtgtc ctactactgt
120agtgccnttt tttggagacc agttcttctg gggcgagaga gttcatgcac
gaggagtggg 180tcctgcacct atatctatng cagaacttac tgtagaagca
ctatcagatg caataatatt 240catgcttgat ccagaggtag tactatttct
tatgctttca ctatatgcat ctggtcatgc 300caacaatgtt tttgatgcag
aactctgctt tggtaaggcc ccattgtgct aataactaat 360ccatttatga
tcctttgctg aaggtaaaat cacgaacaat ggaactggcg atagcaatag
420gcaatgagga tggtgtggca gca 44331201DNAZea
maysmisc_feature(101)..(101)n is a, c, g, or
tmisc_feature(164)..(164)n is a, c, g, or
tmisc_feature(178)..(178)n is a, c, g, or t 31gattttgagg agtcataggt
ggtagggaga agttttgctg gggtgggcag aaggattgat 60ggtaattacc tatgtcatgt
tctacgtgct ctatgtcgaa nggtccggtg tggtacgtgg 120acagtccaga
tgcatactcg ggtagtctgc acgcatagct cganggttcg agcatgtntg
180ttagcccttg catagggtgt g 201321083DNAZea
maysmisc_feature(77)..(78)n is a, c, g, or
tmisc_feature(294)..(297)n is a, c, g, or
tmisc_feature(394)..(394)n is a, c, g, or
tmisc_feature(615)..(620)n is a, c, g, or
tmisc_feature(773)..(773)n is a, c, g, or
tmisc_feature(896)..(897)n is a, c, g, or t 32gtgatgtcag attgtttgga
ataaccagat aaacatctat cgggttgact gattcatatc 60ctttcagtgt tgctgannac
taactgaatg aaagtaggca gacttctgaa ttaattgcaa 120ttttgggaag
tgtaacatac aaaaattatc ttgtgtccga ggaaatggat tgattgattc
180agaaaaccaa ccaccctggt tgtgcaaacc aaaaaaagaa caatatagag
cagtaaaggt 240tgattataac cagcattcca gcaatgaagc caacagtacc
attacaaaag caannnngtc 300aacatgagac atacctgaag gataaggcac
gtatgtcaaa tcacttgcag cccactgaat 360atggaatcat tagtgataga
aactgatatt caanttctat tgcatagagg gtggttacat 420atatagaccc
aggggaaccc taaccctaat gggctagcag cccatttaca catacatgga
480taatatagga gcatacactc taacaatcag tctggtggag aagctggaaa
tacacagtac 540aaaagtagca gaaatagaga aagtacttaa aaatgagaat
ataaaaggtc aaatagttta 600gagttgccat acttnnnnnn gtgaagaata
tgttcctagt caggaaagca atttacggtt 660gccaacgcta gtgaaaaata
tgaaagatat gaatcaagta cagtactatt tgacgaccaa 720atataacctt
gatgaatgtc tcccaacaac cagccaacta ggataaggat ttntaatgtc
780aacccactga aaactaaaga ttcattagca ttctacaaag cagatgttac
aatctaaaat 840atgttcaatc agcagggaaa tggtgataaa acctcaaagc
taatttaaaa cagttnnctg 900cctataaact gctggttctc actaacacat
acatgtacag ttaaatcact ccagtcaacc 960cacttattca gaagaataga
ctgctatgga gctgtgagct atatacgaga gagatcgcca 1020tttatatttg
atcaagtatc tggctttctc tttgaaaggg ggctaaatgc agctgcaggc 1080atg
108333693DNAZea maysmisc_feature(104)..(104)n is a, c, g, or
tmisc_feature(319)..(319)n is a, c, g, or
tmisc_feature(395)..(395)n is a, c, g, or
tmisc_feature(606)..(607)n is a, c, g, or
tmisc_feature(618)..(620)n is a, c, g, or
tmisc_feature(625)..(627)n is a, c, g, or
tmisc_feature(630)..(632)n is a, c, g, or
tmisc_feature(637)..(642)n is a, c, g, or
tmisc_feature(645)..(645)n is a, c, g, or
tmisc_feature(647)..(657)n is a, c, g, or
tmisc_feature(665)..(672)n is a, c, g, or
tmisc_feature(675)..(677)n is a, c, g, or
tmisc_feature(688)..(689)n is a, c, g, or t 33cctgcagacc atatatgttt
atggcatgcc aatatctagt aggaacttag taatagaaac 60gaataaattg ccttcttagt
tttaagaata aaagttggtg catnacatgc agccgctgga 120acaaagtttt
ggctcaaagt catatggtat tgtgaaagca tcactaatat ctagggaaat
180acttcttgaa gaagtgaaga agatcagtaa tgctgttggt agcactcttg
aggatttgga 240tcgcactgac ttaacccttg gtaaatatga gacagttcaa
ccatcaaagt cagcttcgcc 300cagttacagt tatgggcang gtacgcccac
aaagtgtagt ccccagatga ctggcatctt 360acgtgatttt cttgaggtat
acttcacttt ttttnctgaa aaacgttttc tatctttttg 420aatatctgta
ttggttgatg catctcaaac ttgtttcaga gttctggggt tgtggttgga
480agcactgatg atatcttgct gtatactcta tctgaggaag aattgtttga
actatttcaa 540attgtcagca gccaactctc atttatatgg aatgagttct
tgaaattcca taggttagtt 600atcttnncat gcatctcnnn ttagnnnatn
nntgtannnn nngantnnnn nnnnnnnatt 660cttannnnnn nntannnata
aacatatnnt gac 69334201DNAZea maysmisc_feature(16)..(16)n is a, c,
g, or tmisc_feature(19)..(19)n is a, c, g, or
tmisc_feature(101)..(101)n is a, c, g, or
tmisc_feature(135)..(135)n is a, c, g, or
tmisc_feature(155)..(155)n is a, c, g, or t 34aggtaatgag gcaatngtnt
cagcagcaag gcctttgctt tccgtaccga caacttcacc 60cagcgcaatt agctcctgag
gtcaccacgt tcatgaatca nctttgcacg gtagcagaaa 120acaaatgcac
agaanatcct aatctcttca acaanaataa cttgcctcgt aagagtattc
180gtctgggtca acatcttccc a 20135201DNAZea
maysmisc_feature(45)..(45)n is a, c, g, or
tmisc_feature(101)..(101)n is a, c, g, or
tmisc_feature(149)..(149)n is a, c, g, or t 35tgcccttgtt gacgaaggtc
caacgatcag tttcaaaaga aacgnttcca aatgttaaga 60atcagctaga tctaagtgtc
gatgaagatg tcgaatactt nggtgtgttt ggttggagag 120tcaattagaa
tagagtggtt ccattctant ttttgagaat ggagccgttc tattctccgt
180ttggcaagca aaacagagtc a 201361549DNAZea
maysmisc_feature(20)..(20)n is a, c, g, or tmisc_feature(34)..(34)n
is a, c, g, or tmisc_feature(373)..(373)n is a, c, g, or t
36ggctaaaaga attatatatn tgggacagag gggntacgtt ggaagcatct ttatagccag
60cagtcggctg tacttgctca gtcaagaaaa cgcgacatag gcctccctca ccaacactat
120ctgctataaa taaaaggatc ccgccagtac attccctcta cgtggtcatt
gaaccaaatc 180gcagttccta tgtcgacaca tcactgtcaa tttccacgga
agttccagct gcaatccgat 240gcatagatta cacagaatgc atatacttcc
aatagtaggc tatgccatct ccatatcact 300gtgactcatg agtacagtgt
aaacatgaag gcaacgttct gttcgtaagg atttcagaac 360attttccgag
tanctcattt atatgatccg ttattcgatc ttcagatatg gtcctgttat
420tcgatcttca gacttactaa agcaaagcca tgtggagaag aacttcgccc
tctacttggc 480cttcttggaa gcctcgtaca agtagccagc gataactacg
gctgcagcaa ctgctacccc 540aaaggcactc tgtgcaagca cggcggcggt
tggaagatct gatttggatg gtgctgtgct 600gcgaggcgtg aagccaagct
cagagagttt cttgtgtgat tcagcataat ctttgaagaa 660cgcatcttca
tcctttgcat agagctccac atagcgccta aattcaggat ctgataacag
720tgccttgtca gttgggagct ttaaaagtcc ctcagattcc tcattcaaca
gctcaagaaa 780gtatgagtta tcaaacttaa gaggctcctt tgtccaggca
ccatcgaatc cagacctctc 840agggtgagcc cttcccagag tatgtccccc
tgatagagct acaatatctt tgtccgataa 900gcccatccga taaaagatgt
ccctcagatg tggtgcacct ttcttagcat ctggcagacg 960cccttcacgg
gggcacaccg acgaatcacg tctgccagga ataaattcaa cagttggccc
1020cccagtcact tcgactgcaa ccactccagc aagctgatac aggtctgcat
atgttatctt 1080tggattcttt gctttgatag gctcaaggag atcgatagca
atctttaaac cagcatttga 1140accatgagtg tactcttctt catatctaat
cgaaccattt gcaccaccag tttttgtctt 1200caggtcataa gttccagcat
catgccatgc gaggcggagc atgatcgggg cgcatccctt 1260gttggagatg
agggcgcgga ggtggcggcg cgccctgtcg acctggcgca ggtattcggc
1320gtcgaccatg ggagccgcca tcgccggcgc ctcagccgcg gattcctgga
gatcggagag 1380ttggagtgga accgtgggcg gagcggaggc gtgtgggtgg
cgagtgctga ctgactgctg 1440agcagtgggg gatgggaagg gaggaaacga
caactgacga gccgaacaag ataacgcaag 1500cgagcgattc tattggcctc
cagttgccgg cgcctggcaa cccccggcc 154937669DNAZea
maysmisc_feature(41)..(41)n is a, c, g, or
tmisc_feature(115)..(115)n is a, c, g, or
tmisc_feature(299)..(299)n is a, c, g, or
tmisc_feature(332)..(332)n is a, c, g, or
tmisc_feature(336)..(336)n is a, c, g, or
tmisc_feature(348)..(348)n is a, c, g, or
tmisc_feature(350)..(350)n is a, c, g, or
tmisc_feature(370)..(370)n is a, c, g, or
tmisc_feature(491)..(491)n is a, c, g, or
tmisc_feature(495)..(495)n is a, c, g, or
tmisc_feature(527)..(527)n is a, c, g, or
tmisc_feature(534)..(534)n is a, c, g, or
tmisc_feature(576)..(576)n is a, c, g, or
tmisc_feature(643)..(644)n is a, c, g, or
tmisc_feature(646)..(647)n is a, c, g, or
tmisc_feature(649)..(650)n is a, c, g, or
tmisc_feature(664)..(666)n is a, c, g, or t 37acaaatcact cgctactctt
gcctacaccc tcacaatcat ntacgaatac tagtgactgc 60tttcctctcc tcagcatttt
tggcaagtgt tgtgctggcg tgccgtgtgt ggagnggaac 120gctatataaa
gcaacgtcta aaaaagaaaa aaaatactat atattagcat actagtatat
180aaatataaga gtaactccaa tagttttcta aaagactctc taaattaata
atttaagtaa 240ctaaactaaa agctcctctc caacggttct ctaaatgaac
ttcataaatt tagctactnc 300tcatctaacc ttattttctc tctacattta
gnaacnattt accaactncn taaacaaaaa 360aaaattgacn gtaatttttg
tatttcgctg cctttttcac tttatagtaa cgatatatta 420acatagccca
tgcgtcgaac aacgacagtc agctagagat taaataattg ccaatacaat
480agccgcacgt ncacntgtcg gaaataaata aataaacaat tgcaacngta
aatnaaaaga 540tcaacacaac tcaccaagtt gaatatgcca tcgatnatgg
tcccactcag atgagtgaca 600tgttaaattt taacatattt agaaagtaat
atatatataa ctnntnnann agatgcgttt 660tttnnntat 66938684DNAZea
maysmisc_feature(147)..(147)n is a, c, g, or
tmisc_feature(171)..(171)n is a, c, g, or
tmisc_feature(298)..(298)n is a, c, g, or
tmisc_feature(536)..(536)n is a, c, g, or
tmisc_feature(599)..(601)n is a, c, g, or
tmisc_feature(609)..(609)n is a, c, g, or
tmisc_feature(622)..(626)n is a, c, g, or
tmisc_feature(637)..(637)n is a, c, g, or
tmisc_feature(639)..(639)n is a, c, g, or
tmisc_feature(641)..(641)n is a, c, g, or
tmisc_feature(649)..(659)n is a, c, g, or
tmisc_feature(661)..(669)n is a, c, g, or
tmisc_feature(673)..(679)n is a, c, g, or t 38tcgaagctgg gaggctacct
ggcgatattt ttgatgagat accaagcaag tactataatg 60gatcagttgt ttgtgaggta
agttgtttta tcacacataa gaaaggctat gcatgcaatg 120ctattgaact
gcaacatgct gaaaccnttc tgaaatttat tctcttataa nttgtggttg
180gtttctctga tacatggttc aaaaagcatg ctggtacaat ttctgtactg
aggatttatg 240ctatatggga gaaatccttc tttaaaagaa aaactagaag
tttggtgagc tttactgncg 300acatgttcta taatatgcca acctaccagt
actgtaccaa cctttttcta acacttattt 360cagaaacaac cgttatgcat
tatcatgaag catcatgttg tgtcatgtgc tcatggtcat 420gtgctactgt
accttattga taagagacca ttgagtcttt agcagtactt cattgtctta
480catcgacata agtaatgagt tctctttcta gatacatgac taccgaaagc
atgtgnccaa 540ccaagcgcct gcatcatctg ctgagctagg atcaccaatt
gtgaataaag tacgactgnn 600natgacctnt gaaaatgttg tnnnnnacat
taccctncna nctgatgann nnnnnnnnna 660nnnnnnnnnt atnnnnnnna tgct
68439738DNAZea maysmisc_feature(37)..(37)n is a, c, g, or
tmisc_feature(566)..(566)n is a, c, g, or
tmisc_feature(611)..(612)n is a, c, g, or
tmisc_feature(623)..(625)n is a, c, g, or
tmisc_feature(684)..(684)n is a, c, g, or
tmisc_feature(689)..(690)n is a, c, g, or
tmisc_feature(692)..(698)n is a, c, g, or
tmisc_feature(702)..(706)n is a, c, g, or
tmisc_feature(710)..(715)n is a, c, g, or
tmisc_feature(717)..(720)n is a, c, g, or
tmisc_feature(727)..(735)n is a, c, g, or t 39agttggattt ccttctgtag
cttccagtcc tagtgantcc atgcaccgaa tacaacagcc 60tgctgttgct tcctcaaaga
ggaaaacaaa ttctgtcccc aaaactcaac cgcctgtgag 120tgctgttggg
tctccagcca gtgtttcaaa catgcatgcg ctgctgaatg caagcagtcc
180atcgattggg accacaccta tgggagacca agcaatcctt gataaatttg
tgaaaattga 240taacatttcc catcggtata gcataatttc tacatctgct
ccctcccttc acgaattttt 300gttgtcactt tcctttctat tcttagtttc
tttggaagtc tgtcatggga gactttttaa 360ggaagttttg ggttgcacgg
tgtgaatttg atgtctaggc tattttaaag ctgagattca 420gccctattac
tttggatggt cacacaaaaa aatgtcaggc tataatgtgc aaatgaactg
480tttcaattct ttatcaaagt taatcaacat tttaacctaa ataactagtc
cctccagttc 540aaattgtaag ttgttttggt tttttnagat acctggtttt
cactgtgtat atagacatag 600cacacatcta nntgcatagc agnnnctatg
tacctagaaa agtcaaaaca acttacaatt 660tgaaattgag ggaatgccta
acanaatann annnnnnnga gnnnnnacan nnnnntnnnn 720accggannnn nnnnnaaa
73840201DNAZea maysmisc_feature(69)..(69)n is a, c, g, or
tmisc_feature(101)..(101)n is a, c, g, or
tmisc_feature(174)..(174)n is a, c, g, or t 40gtgacacagg tggtgaggtg
ccagccttcg cctttgatgg acgctgtacc tgtacgcgcg 60cgtgttgcng tctgtagagt
agcaggataa ttggacgctc ngatgacacg actgcacatt 120ctcgtacacc
aggctgctat ctgctttcat atatttgacg cgtatgcata cgtncgtgct
180tggactcgta tactactact t 20141201DNAZea
maysmisc_feature(38)..(38)n is a, c, g, or
tmisc_feature(101)..(101)n is a, c, g, or
tmisc_feature(125)..(125)n is a, c, g, or t 41tgaattagga cttctgtttt
ctcactaaac taggtctnta ttttaatctc ttaaacaaac 60ctatgcaaat aaccaaacac
gcttgtgcaa ctaaggtttt ntctaagtgt tgctacctct 120actgnaaaag
gagttttgta acctaagttc caatcctacc aactagtctt tattctaaac
180taagaatggt aacgataaca a 20142201DNAZea
maysmisc_feature(11)..(11)n is a, c, g, or tmisc_feature(65)..(65)n
is a, c, g, or tmisc_feature(101)..(101)n is a,
c, g, or tmisc_feature(152)..(152)n is a, c, g, or t 42aggactcgtc
ngagctggta aacagcgacc attcgcttga cgtggcctcc gagaagtccg 60cgctnaccga
gtctgcgaag gcatcgtcgg cgcgatggaa ntccctggat ctcaactctg
120cttgctgatc ttgcgtgtag ccctcgccat cnggtctaaa gtcataaaca
ccgtatgctg 180gatcgttcgc cgagaggagt a 20143201DNAZea
maysmisc_feature(22)..(22)n is a, c, g, or tmisc_feature(24)..(24)n
is a, c, g, or tmisc_feature(43)..(43)n is a, c, g, or
tmisc_feature(76)..(76)n is a, c, g, or tmisc_feature(101)..(101)n
is a, c, g, or t 43cctacggttg tttgtctttg tncnttgctt gttcacgatc
gantttcacc gaaaacgttt 60ccgatgctgt aacaanatac tcgtttccgt cctagataga
nacaacgtga ttttgttttc 120actaatctcg atgcaaacga gcaacaacaa
aaaaaatcac caaaatgagc acacccagga 180acttgatcgt atctcttcat t
201442607DNAZea maysmisc_feature(2239)..(2239)n is a, c, g, or t
44caggaaaaaa acccggtgtt ctagccgcac gcatctcaaa ggtgatactg ggcggggcga
60tttggagatg atgttctagc actagcagca cgcacgcatc tcaaaggtga tactgggcgg
120ggcgatttgg agatgatgtt ctagcactag cagcacgcac gcatctcaag
gtcatactgg 180gcggggcgat ttgcgagtcc tgtggcctcg tgggccacgt
gcacgcagca gaccgcagga 240atttgcacga gaccagtacc tagaacaccc
tactccagcg aaatccgcca cttcgtcgtc 300gtcgtcgcac ggattggcat
tggctgcccc ccggccctgt tcctcgtcct cctcatcaat 360tcccgtctct
cagtcctccc aataatggtg gcgtggtgag aaatccttcc gtccggccgt
420cgtcttagaa gatgaactgt tcctcttgct gcgccgctgc cactccatct
tctccggcgc 480ttctcgccag gccgcggggg ggtttagctg ctagctgctc
cacgacgaca gcaaatcaga 540aggtgctttt cctgggctca aaacagtttc
cacggatcac gtatagccgt gcgtcgtcac 600ggttgtcgcg gagagaggta
atagcttttg ccgggcaaca accttgggac atcggcagat 660ttgtcaagac
gctgtatttc ttcaacgggc ctccaaacct tctcaagatt gtagaatcta
720tcatcagcag tttcactgga cctgcttcta gtgaagtgcc aaagaaaatg
gaaacgtcgg 780atgtggtgct ggttactgga gccaccggtg gtgttgggcg
acgggtggtg gacgtcctgc 840agaagaaggg agtacctgtt cgagtattgg
ctagaaatgt agacaaggca aggagcatgt 900tggggccgga tgtacctctg
atcataggag atgttacgaa ggaagataca cttgatccta 960agctattcaa
agggataaaa aaagtagtca atgcagtctc tgtcatagtg gggccaaagg
1020aaggtgatac gccagacagg cagaagtaca aacaaggcat caaatttttc
gaacctgaga 1080tcaagggacc ttcacctgaa atggttgagt acatcggaat
gcaaaacttg attaatgcca 1140taaagagtag tgttggactg agtgaaggga
aactgctatt tggtttcaaa ggcaacttat 1200ctggagagat tgtgtgggga
gctcttgatg acgttgtgat gggtggtgtt agtgaaagta 1260cattccaaat
cttgccaaca ggaagtgaaa gtagtggacc aactgggttg ttcaaaggga
1320ctgtatctac ttcaaataat ggtgggttta ctagtataag gacaaagaat
tttactgtgc 1380cagaggacct gtcagcatat gatggtattg agttacgagt
taatggtgat gggcgacggt 1440ataaactcat tatacggact agctatgaat
gggatactgt tggctataca gcaagtttta 1500acacaactaa ggggggatgg
caaagtgtta aagtaccttt ctcttctctg aaacctgtat 1560tccgtgctcg
tactgtgact gatgctccac ccttcgatgc aagcaacatt acttcactac
1620aactcatgtt tagcaaattt gaatacgatg gaatactcaa cccaacattt
actgaaggtc 1680cgtttgagct tcctttttcg agtattagag catacatcaa
tgagccgatc actccaaggt 1740tcgttcatgt gagttctgcg ggagttacaa
gacctgaaag accggggtta gatttaagca 1800agcagccacc tgctgttcga
ttgaacaaag agcttggctc cattttaact tttaagttga 1860agggagagga
tttaattcgg gaaagcggta ttccgtacac tattgtaagg ccatgtgcat
1920taactgagga accagctgga gccgatctca tgtttgacca gggggacaac
atcacaggaa 1980agatatcaag ggaagaagtt gcccgtattt gtgtagcagc
tctggcaagc ccagatgctg 2040tgggcaaaac tttcgaggtc aagagcactg
ttccattcag cgaaccgtat gtgattgacc 2100ctgcaaatcc tcctcctgaa
aaggactatg aagtatattt caaagaactc aaagaaggca 2160tcacgggtaa
agaggcgtta gaggcaacac ctgctcaagt ttgaagatgt cgttgaatta
2220agaatttcgt ctgttttcnt aaattctgac acagtaaccc ccactctgaa
tgtctaaagt 2280catctagaaa catagatgac actaatgcta aattttgtag
ctgaagatca gaccaataaa 2340tattcccaag aacacattac tgttaggttg
ggggatcatt aaactcgagc taagacacaa 2400atatgtagat gaaattagtg
ctgctgttca tgccttcgtt ggagttagcc attacaggac 2460acaagaattt
agtaagaggt ttggcaaaaa aaaaaaaaaa aaggagcagc tgcctgatga
2520gttggtagag tggttgcttt cagtcaattc tctagggtgc ctatctctat
ttaacttgat 2580gctgcacact gggggctccc ttcagtc 260745608DNAZea
maysmisc_feature(115)..(124)n is a, c, g, or
tmisc_feature(210)..(210)n is a, c, g, or
tmisc_feature(231)..(231)n is a, c, g, or
tmisc_feature(350)..(351)n is a, c, g, or
tmisc_feature(358)..(360)n is a, c, g, or
tmisc_feature(562)..(563)n is a, c, g, or
tmisc_feature(569)..(569)n is a, c, g, or t 45ttatgctagt tagattttgc
aaagaaaagt tcaattaccc cagttatctt gtttccctga 60atccttggta cacttgtagt
ttcttcttaa ttatgtaact ttaggttata taatnnnnnn 120nnnngctaca
tcttgattgc tgctgttcct ataataatat tccatgattt ggcatttttg
180gcttcattta gctgaaccaa ctgtaatctn acttcttatc acccccgggc
nttaatgatg 240cttctgtagc caagggtggg ctgagggagg cttttgcagc
tccagggtta ggttacgttg 300atattccaaa tgcacagata agaaaggtaa
attttgatgg ccagaaacan nttttttnnn 360catattagat gttagtaact
tgagcatgtg ttgatttctg ttgtcttcag gttaccgcaa 420accgcttgct
agcatctaaa cagaccattc ctcattacta cttgacagta gatgcacgtg
480ttgacaaact tgtcaagtat gtttctaacc taatatatga tttacagagc
tcttttcgtt 540ggtgggacag cacttctaac tnngagttnt gtcattctaa
tccacaggtt gcgaggtgaa 600ctgaatcc 60846201DNAZea
maysmisc_feature(47)..(47)n is a, c, g, or
tmisc_feature(101)..(101)n is a, c, g, or
tmisc_feature(167)..(167)n is a, c, g, or
tmisc_feature(178)..(178)n is a, c, g, or t 46ttggagtgat acttgttcga
ggaagagccg tggtggtggc gaaaacntcc atgttctctc 60gtcttgtcaa ttgtcacaag
ctaggacatc gatcgccgat ngagctagct aggggtcgtc 120gacggcgagg
ccgccccagc tagctagcta ctctcgatca ggggaanctg cttggtcngg
180acccgcgccc agacgacgac g 201471294DNAZea
maysmisc_feature(152)..(152)n is a, c, g, or
tmisc_feature(203)..(203)n is a, c, g, or
tmisc_feature(240)..(240)n is a, c, g, or
tmisc_feature(317)..(317)n is a, c, g, or
tmisc_feature(345)..(345)n is a, c, g, or
tmisc_feature(402)..(402)n is a, c, g, or
tmisc_feature(406)..(406)n is a, c, g, or
tmisc_feature(418)..(418)n is a, c, g, or
tmisc_feature(471)..(471)n is a, c, g, or
tmisc_feature(473)..(473)n is a, c, g, or
tmisc_feature(483)..(483)n is a, c, g, or
tmisc_feature(493)..(493)n is a, c, g, or
tmisc_feature(525)..(525)n is a, c, g, or
tmisc_feature(536)..(536)n is a, c, g, or
tmisc_feature(553)..(553)n is a, c, g, or
tmisc_feature(558)..(559)n is a, c, g, or
tmisc_feature(574)..(574)n is a, c, g, or
tmisc_feature(576)..(580)n is a, c, g, or
tmisc_feature(586)..(586)n is a, c, g, or
tmisc_feature(589)..(589)n is a, c, g, or
tmisc_feature(620)..(622)n is a, c, g, or
tmisc_feature(625)..(627)n is a, c, g, or
tmisc_feature(634)..(644)n is a, c, g, or
tmisc_feature(647)..(650)n is a, c, g, or
tmisc_feature(652)..(681)n is a, c, g, or
tmisc_feature(687)..(702)n is a, c, g, or
tmisc_feature(704)..(720)n is a, c, g, or
tmisc_feature(722)..(723)n is a, c, g, or
tmisc_feature(725)..(725)n is a, c, g, or
tmisc_feature(729)..(730)n is a, c, g, or
tmisc_feature(734)..(745)n is a, c, g, or
tmisc_feature(747)..(755)n is a, c, g, or
tmisc_feature(757)..(765)n is a, c, g, or
tmisc_feature(770)..(771)n is a, c, g, or
tmisc_feature(779)..(782)n is a, c, g, or
tmisc_feature(784)..(791)n is a, c, g, or
tmisc_feature(798)..(799)n is a, c, g, or
tmisc_feature(801)..(801)n is a, c, g, or
tmisc_feature(805)..(812)n is a, c, g, or
tmisc_feature(816)..(824)n is a, c, g, or
tmisc_feature(831)..(836)n is a, c, g, or
tmisc_feature(838)..(838)n is a, c, g, or t 47ttgttgacag cggagcgcaa
tcgactatca tatcaaaaag ttgcgctgaa cgttgcgggt 60atgattataa tttagttgat
ttgagctgct tagattttat atttgcagca ccatggtttt 120tgtgattttc
agcatggaga acataatatt angtagcaat cagccaatca tagggggtgt
180ttgctatagc tctgcttcac tantgaagta gctttgctgg aggagctgtt
ctggaatagn 240tctgctggtg catttgaagc tgtttttcta gaccatttgg
taaaacagct tctcatgata 300gataaataag gcaaaanggc ctctatccga
gttggttagg tggtntgggt agcactcctt 360aggtcctgag ttcgaatccc
agtggaagcg aattttaggc tnaggnttaa aaaatgtnac 420tcgttggttc
ccctggtcgt gtgcacacaa gatggactga cctatggggg ncngatcctc
480gtntaggggc tgngagggct taaatcacga gtaaagatct ggtcnatagg
ggatgnaccc 540tcatgttgca cgnggganna actttcgtga cctntnnnnn
tcaggnctnc gattgagctt 600cttcttaata taataccgtn nnagnnntct
tttnnnnnnn nnnngannnn tnnnnnnnnn 660nnnnnnnnnn nnnnnnnnnn
nactacnnnn nnnnnnnnnn nntnnnnnnn nnnnnnnnnn 720cnncntctnn
atcnnnnnnn nnnnntnnnn nnnnncnnnn nnnnncgctn nattccgann
780nngnnnnnnn ntgtgtcnng ncatnnnnnn nnaccnnnnn nnnntacgcc
nnnnnncnct 840gcgctaggag ctccagcgac cccacgccag cacaacttct
tcacgttgcc acgcctcccc 900tcgctgtcac cgcgcgcgcc cctggggcaa
attcgactat gctcacatga ggccccacgg 960agacaagtga gagcagggtg
aaggtgcctt tttcagctcc acctcacacc tttgacttgt 1020gtgagcagaa
aaaaacggga cttcacatgt ggagctgcaa cctccctaac cgtttggctg
1080ttgttcttgt gagactgctt cgggagttgt cccaggagcg gtaacaaacg
gcctcttagt 1140ttataaagta cttcctccgg cccaaaataa atcaattcct
agaatcaccc ttagccaaac 1200tattaagttt gaccaacttc ataggaatgg
gtatgtgcac caaataagta cattacgagg 1260ctgttgcttt gaattaaagc
tagttgttcg gact 129448332DNAZea maysmisc_feature(247)..(247)n is a,
c, g, or t 48gtcaagtccg gcgcctccaa ggtggccgtc aacgacgtcg tcttcaagaa
catccacggc 60acctccaaca cgccggaggc catcacgctc aactgcgcca acaacctgcc
ctgccagggc 120gtgcagctca tcaacgtcga catcaagtac aacaggtccg
acaacaagac catgtccgtc 180tgcaagaacg ccatcggcaa gtccattggc
atggcgaagg agctcgcctg cgtctgaacc 240tacttgnatc catcactcac
tcttcgtcac ctctctcttt ctcactctcg ccagtctttt 300tttaggcctc
tggcaatctg cgaactttct ta 33249776DNAZea
maysmisc_feature(719)..(719)n is a, c, g, or t 49gcgggcacga
cgtgtccaaa ctcaaccacg cgctcgctca aggcacacgc caatccctcc 60cagcgcaccg
ctctcgtcga agcccgcaag tacgttgcct tctacaccct ttcgcccagc
120gctcaggcct tgaagggtcc ctttctgtgt gtagcttgcc ttgaagggtg
tccaaacttg 180tgttttgttt gctctggatt tctttacctc tctctgggga
ggagacaagt tgctgcaata 240acgttatgct ctggattctg aatcatgtgc
cttcatcgtt aggttgattt tcctgattca 300gaaagatgat cgaggcaatc
tacgagtacc gaggcgagga tccgctccaa ccgtggctgg 360agtaagcgat
tctcgatgcc tctctttagc tcgcctcttt cgatttgttc gttcgtggtt
420gctaccctag ccgaaagatt gcttatctct tctctgcttc gtccgcgcgc
agctgcatca 480agtgggtcca ggagtcattc ccgaccggcg gcgacagtgc
gtgcggacct tatggcacga 540cgagcgctac aaggacgaca tccgctttct
caaagtgtgg ctggaatacg tgagtcgtgc 600gatggcagct tgattttttt
tcctgtagtt ttagtctgtg cagtgaaggc atctcacata 660tcattccgct
gccgatcttg ttctctttgt aggctgggaa ctgtgctgat gccgaggtna
720catacaggtt cctggaggcc aaccagattg ggcagggcca tgccatctac tacatg
77650612DNAZea maysmisc_feature(1)..(2)n is a, c, g, or
tmisc_feature(200)..(200)n is a, c, g, or
tmisc_feature(244)..(244)n is a, c, g, or
tmisc_feature(257)..(257)n is a, c, g, or
tmisc_feature(429)..(429)n is a, c, g, or
tmisc_feature(451)..(451)n is a, c, g, or
tmisc_feature(593)..(594)n is a, c, g, or
tmisc_feature(599)..(599)n is a, c, g, or
tmisc_feature(602)..(606)n is a, c, g, or t 50nnatctctga ttctgttcgc
tccaaattaa ttacatgctc ttttctcttt tcaggaggct 60tctcaatggc aactgccaac
agttcgtgat tagtatacac aagcattatg gtaacaaaga 120atcgattagt
cagatttgca acagcatata tacctggtac agctaaattt atttcacggg
180gtttctttct ttcaggaacn tcagataaac ctgttagtga aacttgaaaa
tcatgtatca 240tganatgaaa acaacanaag aaaacacata aatacattac
acaatggtca atgaaagtta 300accttttttg gcatcatgtc tagactgcac
cgaagcttgc ttgctttttg gctcctcagc 360cctctgatgc ctacctaatt
gagccatgga ataatcacgt ctagtctgtc tttcttgata 420cttggacang
caaaatacta ccagtagcgc nagaactatg aatagcacaa tagcaagaag
480aacatatcca acagttctga gggttgaagg cttcttcttc ttggatgacg
tagaattgct 540atttcgagca gtagatccac tagaagagtt tgatgctgca
ttagatggtg aannagatnc 600tnnnnnggat ga 61251755DNAZea
maysmisc_feature(1)..(1)n is a, c, g, or tmisc_feature(10)..(10)n
is a, c, g, or tmisc_feature(101)..(101)n is a, c, g, or
tmisc_feature(214)..(214)n is a, c, g, or
tmisc_feature(248)..(252)n is a, c, g, or
tmisc_feature(320)..(331)n is a, c, g, or
tmisc_feature(345)..(345)n is a, c, g, or
tmisc_feature(388)..(390)n is a, c, g, or
tmisc_feature(496)..(496)n is a, c, g, or
tmisc_feature(665)..(671)n is a, c, g, or
tmisc_feature(707)..(709)n is a, c, g, or
tmisc_feature(712)..(713)n is a, c, g, or
tmisc_feature(726)..(727)n is a, c, g, or
tmisc_feature(729)..(729)n is a, c, g, or
tmisc_feature(736)..(736)n is a, c, g, or
tmisc_feature(740)..(747)n is a, c, g, or t 51ngcctgcagn tgcttaaact
caccgaaaca aatatatcgg tcccagctgg tatttcatat 60ccctcttttg ctccattgca
cccgcctggg taaatagacg ntcacggcgg cagagttaga 120aggcatgtac
cagcgaaggc aacgcaccct gcttgtcact aaactaattc attgaaaaat
180agttgtgtgt gctgatcgaa ggatacaatt tgcnaacatc catccacaag
tatagaaagg 240ctgtcatnnn nncaccaagg tctcgataaa catattacca
gtaacaagaa cagcgacttc 300aaccaaacta gtctaaatgn nnnnnnnnnn
naccactaca caccntttgt attgctcagg 360aaagaactta cgacattaca
gagaagannn tggtacctgg caacttgtct ggccggagag 420aacgcctgat
taacaatggt ggctgaggat acaagcgaag agcttcaaga ataatcagtt
480ttatgtacct agaggncaac acaaaggttt catgagagat gcaaattcat
ttcaaatgta 540tagatatagc tagtaatttc agagaagtcg gataccatgg
atatgctact gttaataatc 600tgataatatt ttttatgaaa caataatcct
tattgataat atttttatga aacaataatc 660cttannnnnn ngcaccatac
acctatccct caataaatgg aaaatannng anncaattct 720agatgnntna
aaaatnatgn nnnnnnngac atact 75552399DNAZea
maysmisc_feature(5)..(5)n is a, c, g, or tmisc_feature(28)..(28)n
is a, c, g, or tmisc_feature(81)..(81)n is a, c, g, or
tmisc_feature(91)..(91)n is a, c, g, or tmisc_feature(168)..(168)n
is a, c, g, or tmisc_feature(180)..(180)n is a, c, g, or
tmisc_feature(208)..(208)n is a, c, g, or
tmisc_feature(214)..(214)n is a, c, g, or
tmisc_feature(222)..(222)n is a, c, g, or
tmisc_feature(227)..(227)n is a, c, g, or
tmisc_feature(231)..(231)n is a, c, g, or
tmisc_feature(248)..(248)n is a, c, g, or
tmisc_feature(256)..(256)n is a, c, g, or
tmisc_feature(286)..(286)n is a, c, g, or
tmisc_feature(310)..(310)n is a, c, g, or
tmisc_feature(337)..(337)n is a, c, g, or
tmisc_feature(361)..(361)n is a, c, g, or t 52gatcnggcca ggcactggct
acaccgcntt gattggggtc ctggggctcg aaggaatacc 60accaaacctg ttgaccagga
ncttgaaaca ngggaacgaa acggcgtaca gcacgaccag 120tacaattggt
gcagcaataa gccaacccag catctgttgt cgacaatngc agtagtcttn
180tgtcaatgtc ccagcaaagc aagagtanaa cagngctcct ancatangaa
ngagcaggac 240tgatttgngg aaaggntgtt cactcaccgc atggacgatg
ctcatnagca catcctttga 300ggcatgaccn gtgagaacat tcttcaatgc
gtcggcngtc aaggggaagt gcccgctgcc 360ngtgacggct tcacccaaac
gcaagaaagg gacgatcaa 39953448DNAZea maysmisc_feature(62)..(62)n is
a, c, g, or tmisc_feature(316)..(316)n is a, c, g, or t
53agtacaaccc tgacggcgcc atctggggca acaagatcgc gtggggccac gccgtgtccc
60gngacctgat ccactggcgc cacctcccgc tggccatggt gcccgaccag tggtacgaca
120ccaacggcgt gtggacgggg tccgccacca cgctccccga cggccgcctc
gccatgctct 180acacgggctc caccaacgcc tccgtccagg tgcagtgcct
ggccgtgccc gccgacgacg 240ccgacccgct gctcaccaac tggaccaagt
acgagggcaa cccggtgctg tacccgcccc 300cgggcatcgg gcccanggac
ttccgcgacc ccaccacggc ctggatcgac ccctcggacg 360gcgcatggcg
cgtcgtcatc ggctccaagg acgacgacgg ccacgcgggc atcgccgtcg
420tctaccgcac cacggacctg gtgcactt 44854226DNAZea
maysmisc_feature(167)..(167)n is a, c, g, or t 54ggcttgttga
agttcttgta cccctgggca acaatggcgc acgccatgga tgagaagcaa 60gcatcaagca
gcagcgagca gcaatagacg gatggtgatg gagatggaga ctcgggacac
120ggaccacgcc ggagacgtca aggatggtgg tgctctggtc gacgtgnttc
ttggcggaga 180tggagcaggc ggggaacttg acggcgaagg cgcgctcgaa ctccct
22655398DNAZea maysmisc_feature(99)..(99)n is a, c, g, or t
55ttttgagtca tcgggtcgat ctgacgattt gctatgggtg ctctgagtac tcgacctggg
60aactgtgggg cgcttgtagg cggcgcagag ttgggtctng tcgttgtaat gattccatcg
120gctcgttgcg tagggtttga tctgcctgtc gttttctttt cttttaatta
aatttctgat 180gcacagtcat gtattgatct ggtttggtgt tcctagatgc
gtgtaataaa tggggaaaaa 240accatgtcgt aataaaatca atttattttt
aataaaaaaa atctgaattc tggctctagt 300gaataaatgg atagagcctg
gctctaattc tggtaaaata aaaagacaac gagcttaact 360tcttaattgg
tcaattggaa gggttctccg atctaatt 39856521DNAZea
maysmisc_feature(390)..(390)n is a, c, g, or t 56cgtctgcgcg
gtcctgcatt gaaaaaaata aacgcatggt tcacataaaa atacgggacc 60gtaatgctga
ctactgtcga atataaaaaa aaagatgtat aggaccatac cggcctaaac
120atgtccacga caatgtcatc cagaccacca ctaagtcccc tttgcagcct
acctgtcccc 180atgagttcgt tgttgtagtt tagatgactg ctgacagctt
ggtgatcaat cctgccatct 240tgcagagggg ggacaacaca accacctgaa
ttgctaccac ccagtgtctg ccctggaaca 300acagaaccgt ctatgcgaag
gttcggcaat gtagagttga cgcctgaatt actaagcgca 360aggttgttat
tcagcatctc tctcgagttn gtgctgcttc cataagcgaa gggcactgct
420tcctgagtga acggcatcgg aagaccgttg ctggctagtg atgctacttg
gtggctctgc 480atttcatttc ccagcaaagg gatctgactg gatgcttctg t
52157783DNAZea maysmisc_feature(279)..(279)n is a, c, g, or t
57cgctcagcgc gggcgcgacg tgtccaaact caaccacgcg ctcaaggcac acgccgatcc
60cgcccagcgc gccactcttc tcgaagcccg aaagtacgtt tccttctcca gtctttcgcc
120cagcaatggg ggaaaaggag ttgtggtttt gtaacttttg tttgctctgg
atttcttttt 180cttgggcctg ggtctgtggg gaggagacaa gttgctgcag
taatgcaaat atatatcatg 240tgtgttcatc gttcggttca tttgcttgat
ccaggaagnt gattgaggca atctacgagt 300accaaggcga ggatccgctc
caaccgtggc tggagtaagt ttttctcata gcccctttaa 360ctcgcctttt
tcgatttctt ggttcgtgcc aggactggta gctgttctag cagatagatt
420gttcatttct tctctgattc gtccgcgtgc agctgcatca agtgggtgca
ggagtatttt 480ccgaccggcg gcgagtgctc
agggttggtg gtgttgtacg agcagtgcgt gcggacctta 540ttggacgacg
agcgctacaa ggacgacctc cgcttcctca aagtgtggct ggaatacgtg
600agtgatgctt tgccagcttg attgtttttc tctgtagttt gtgtcagtgc
agtggaggca 660cctcatatat cattccgctg ccgatcttgt cctttttgca
ggcggggaac tgtgctgatg 720ctgaggtaat atacaggttc ctggaggcca
accagattgg gcagggccat gcgatctact 780act 78358201DNAZea
maysmisc_feature(5)..(5)n is a, c, g, or tmisc_feature(101)..(101)n
is a, c, g, or tmisc_feature(162)..(162)n is a, c, g, or
tmisc_feature(164)..(164)n is a, c, g, or
tmisc_feature(169)..(169)n is a, c, g, or t 58aaacngacat ccccaagcaa
accgagtgca atacgataca agtagagatg tagaacatca 60ccttggttag gccggatctt
ggtagcgaac actaacaacc ngatttgtca ccctgatctg 120aaggccatgt
atcaaacaca aatcattaga acaattgcaa cncnttcgna ttactcatgc
180atataaggaa acaatcacgg a 20159121DNAZea
maysmisc_feature(61)..(61)n is a, c, g, or
tmisc_feature(100)..(100)n is a, c, g, or t 59gtacaattaa tgctcgtaag
aaattgcaat aaataaaata aaaaagagaa atttactatg 60nttcattgtt cttgtaccga
aatggcctgg tcggccctcn tggcctagcc caatagtctg 120t 12160669DNAZea
maysmisc_feature(63)..(63)n is a, c, g, or tmisc_feature(81)..(82)n
is a, c, g, or tmisc_feature(85)..(85)n is a, c, g, or
tmisc_feature(131)..(132)n is a, c, g, or
tmisc_feature(277)..(277)n is a, c, g, or
tmisc_feature(339)..(339)n is a, c, g, or
tmisc_feature(370)..(370)n is a, c, g, or
tmisc_feature(378)..(378)n is a, c, g, or
tmisc_feature(382)..(382)n is a, c, g, or
tmisc_feature(385)..(385)n is a, c, g, or
tmisc_feature(391)..(391)n is a, c, g, or
tmisc_feature(433)..(433)n is a, c, g, or
tmisc_feature(601)..(602)n is a, c, g, or
tmisc_feature(621)..(624)n is a, c, g, or
tmisc_feature(626)..(626)n is a, c, g, or
tmisc_feature(630)..(634)n is a, c, g, or
tmisc_feature(637)..(637)n is a, c, g, or
tmisc_feature(643)..(655)n is a, c, g, or
tmisc_feature(657)..(658)n is a, c, g, or
tmisc_feature(661)..(666)n is a, c, g, or t 60ttgcttcatc gacggccggg
acggcgcgtc gtccccggtg cacatgaccc cgagcttgaa 60canggccacg gcgtcgtcga
nntanacggc cctgtcctgg atggtctcgt ccacgacgtc 120gtggagaggg
nnccccgcct tgtacctccg ccacgcccac tccaccaggc agcagtcagc
180ggcgtccttg ctgctgtcgt tggctaccct cccggtcgcc agctccagca
gcaccacgcc 240gaagctgtac acgtctacct tctggttcac cttggcnccg
cgtccgtact ctgcatccaa 300ttccaaccat agcagatcag cagagtgtcg
attcatttna gcctcctgag aagaccagaa 360gggttggccn gctggtgnct
gncgnacggc ngattaagaa cgcacctgga gccatgtatc 420cgaaggtgcc
acngacggcg gacacggact cgggctcgcc ggacttgagc aggatccggg
480cgagcccgaa gtcggcgatc ttggcacgga accccgggtc gagcaggatg
ttgctggact 540tgacgtcccg gtgcatgatg ggctgcgcgc actcgtcgtg
catgtagctg agccccctcg 600nngcgtcgat ggcgatgccc nnnngngtcn
nnnngtncag cgnnnnnnnn nnnnngnngt 660nnnnnnggt 66961393DNAZea
maysmisc_feature(125)..(125)n is a, c, g, or t 61tcagattaac
atatgtgctt tacaaaattt tctatcttgt ttatttccta gtaggaactc 60caaagcatga
gtagaaaagg aattgatttg gtttttgtta gttattattg aaagagaaat
120caacngtttg ctagtaatgt tgtgactcaa tcatttctgt cggtgtacaa
tcatattgca 180caaaattatt tacagccttt gtaaatgcaa gcatattagt
tctgtttgac taccttgatg 240ggttctacac ttgtactctg ttgttatttt
acctttgatg gaagtttctt tgtaaggaaa 300tttctatagt aacttctagc
ctatatgtca tagaacctct gttgcaataa tacctttctt 360tcatcagtta
caaacccaaa tgcgttctaa aga 39362201DNAZea
maysmisc_feature(26)..(26)n is a, c, g, or tmisc_feature(72)..(72)n
is a, c, g, or tmisc_feature(101)..(101)n is a, c, g, or
tmisc_feature(156)..(156)n is a, c, g, or
tmisc_feature(161)..(161)n is a, c, g, or
tmisc_feature(187)..(187)n is a, c, g, or
tmisc_feature(195)..(195)n is a, c, g, or t 62agtgagagcc aaggaacata
agacanagtg atcaaccata ggtttagcaa agcaatatgt 60tcatggtgat cnactagacc
cttgattttg gcgtacatat ngatatattg actctagttc 120ttagtggtgg
ccttgtcaac tatcttgtgg gtgcanctcg naagggtgaa catagggcat
180ggggaantga tatcnagccc a 20163467DNAZea
maysmisc_feature(245)..(245)n is a, c, g, or
tmisc_feature(332)..(332)n is a, c, g, or
tmisc_feature(369)..(369)n is a, c, g, or
tmisc_feature(452)..(452)n is a, c, g, or t 63ttctattgat tgcagtttta
tattgtgatg ttgtggaggc atatgagtac catttttttt 60tcttcttaat atagcactag
tgttctgata ggcgcaggaa atcaacagct cgtgcagatg 120ggtttcttcg
agcaccctct cttgtcgaca accttgttat attttagaag ttatttgttt
180gacatccatc catccatctt ctctccaaat ttaggtcgga tgtacgagct
ctatcgacgt 240tatanttgtg cctccgcgag tccgagctga cccagcagcg
agtcaggtac cagcagtaga 300gttgtttgtt ttgtcgtccc atactgaaaa
tntatcatct atgcacatct cttgcctgat 360ccccctttng ccttctgtat
cgtggttcag aatccagcag ttgttgccag gaggatggtt 420ctgaagtcac
cacgcctaag gatcaaaagg cntgtcgtag tggcaca 46764201DNAZea
maysmisc_feature(101)..(101)n is a, c, g, or
tmisc_feature(128)..(128)n is a, c, g, or
tmisc_feature(163)..(163)n is a, c, g, or
tmisc_feature(191)..(191)n is a, c, g, or t 64agtgactata agaataatgg
aataaacgat gacccagtgt gtgtatgatt tccgttctga 60agaacaacac atcccttgca
ggattgttgt tcagtagatt ntacttgtac cgcggctttg 120gagttccnca
aaggacttta cttgttcaag aacttcttct ctnacaggaa ataacatgtg
180agtgcagtcc ntgcttttat c 20165201DNAZea
maysmisc_feature(101)..(101)n is a, c, g, or
tmisc_feature(188)..(188)n is a, c, g, or t 65cgccgcgtcg gcgcgccttt
ccgctggata tcccttgcca ccgccgcaaa ctgcttgcag 60cgtctttttg cattattgcc
tacacaaggc agaagacacg ntgattagaa tgcagcacgt 120acgtgatatc
agagtacaac cacctacaca atggacacgc agagatctct tccgtttctg
180ggcacagngc tttccatggg c 20166201DNAZea
maysmisc_feature(19)..(19)n is a, c, g, or tmisc_feature(47)..(47)n
is a, c, g, or tmisc_feature(101)..(101)n is a, c, g, or
tmisc_feature(173)..(173)n is a, c, g, or t 66agaattgcta ccatgcatnt
tggggtcctg ctatgtattg ggttcantct atggagaata 60gaatcaagga actgagttgg
gaagcgcttt gtgccaccct naacactaga tagatcagca 120taacctgctc
attagacatt tttatcacat ccatcaaacc aacttagtaa ccnaatatgt
180ggaacaattt gaccaactta t 20167201DNAZea maysmisc_feature(4)..(4)n
is a, c, g, or tmisc_feature(17)..(17)n is a, c, g, or
tmisc_feature(76)..(76)n is a, c, g, or tmisc_feature(101)..(101)n
is a, c, g, or tmisc_feature(194)..(194)n is a, c, g, or t
67ctgntttttc atatctngac attgtgattt agtaagttgc tcatatattc tactacattc
60tttagcacgc tttccncaag gggggcagtt ttaggcattc ntactgtggt tctgaatgaa
120gagataaatg atactccctc attcctgaag tctctgaccg aatttataga
aattagtacc 180aacatttctc tctncaaata a 20168201DNAZea
maysmisc_feature(101)..(101)n is a, c, g, or
tmisc_feature(123)..(123)n is a, c, g, or
tmisc_feature(130)..(130)n is a, c, g, or t 68ttagagacta atctttaacc
cctctaacca aacaccatct aaattaaatt aggtcgcaat 60acatgattta ggactccttg
gagttgctct aacgttccca nttgtcggac gccttcagta 120tcnggttggn
cattttaaca aagcaagtgc attcgagaga cccattttta caagttcgat
180gagagtcggc ctattttaga g 20169201DNAZea
maysmisc_feature(69)..(69)n is a, c, g, or
tmisc_feature(101)..(101)n is a, c, g, or
tmisc_feature(165)..(165)n is a, c, g, or
tmisc_feature(185)..(185)n is a, c, g, or t 69aaagctacat ccgaattgct
gaattcatga tatgttcagc agttgcaact tcttcgcctc 60atattcagna gggcactctg
tccaattata caagctagat nttcaaattt tgtcacgata 120ccactcattt
gttgcaaaca ctacactaat gtcaccctgt tgatncaaaa gtctccaaca
180tgagnaacat tttgcagtcc a 20170201DNAZea
maysmisc_feature(101)..(101)n is a, c, g, or t 70atgccatcta
gttgctcctt ctccacaagt ctgaaaaaca atgaacttat tcctatatga 60attcttaata
gaggtccttc tatatgacaa acagctctcc nttgtcgtag agggagggac
120tgtagcatac ctgtattgaa gaactgcatg ttgcttgctg caggagggat
gatctgtggg 180gacatctgca actttccttt c 20171201DNAZea
maysmisc_feature(101)..(101)n is a, c, g, or t 71gaagtttgaa
acaatggaat ctacatttag atttccgcta aagcttttgt cttccctatc 60agagcttaag
tttcttatta ataatgatag ggtaattcta ntagcacgat caccaaggtt
120gtgtttggat tggggactta gaggatggga tggttccatt cctgtttttg
aggatgcctt 180tagtttggtt gagagggtct g 20172854DNAZea
maysmisc_feature(5)..(12)n is a, c, g, or
tmisc_feature(279)..(279)n is a, c, g, or
tmisc_feature(315)..(315)n is a, c, g, or t 72tgcannnnnn nngctgttaa
tctcacatcc tgctctgtcc agaacccttc ctcagccatg 60gcataactca aatcaagaat
cttctcaaag agtgtcctct tgtttgacct ccactcattc 120aggaacttga
tcaaccgacc aacattcaca tgaagatcct tctcttctgc aaatgggtat
180gcctgaatat agtccttcct gtaaatagta ggtggataga aagccacata
tccaccaatc 240tcccacaaga tccgttgcgc ccagtaccca cggatcacnt
ctgctgccat tgagctcaca 300gacactggca tcatnagccc ccagaaagca
ggtgactgga acaatgtatt gaacgagttc 360actggtgcca tcatgccctg
aggtagtgcc accttcgggg cttccgaatc aaacctcaga 420tcgaacgccg
aagttggtgg ttttctagtg aagtagaaca ccgcatctac atctggcaac
480ccgtccgaca gcccctgctg aatgaattgc cggccactga agacctcggt
gtagaacacc 540tcatgggcga cctccccaac cttatcgagc ggcagccccc
ttggccagac cgaccgctgg 600ccgaagtgca cgtaggggtt caccactgtg
cgattgggat ccgcgtggct gtactggagc 660agcactgggt ggttggtgac
tccagatccc aaatccacgt cgaaatgctt ccccagatcg 720ttcccgggca
cctcggcgcg gtcgtcggcg tcgaagatca ccttggcccc gtgctggatg
780gcgaagaggt agcccgccgt cttgcggacg tgggagccgt agggcaggaa
gtcgacggag 840cggtatccga gctg 85473440DNAZea
maysmisc_feature(265)..(265)n is a, c, g, or t 73tacactggta
tatgatacac tgaaacccaa aaaggtgtca ggaggcataa ttatacatgt 60aaaccaaaga
agaaaaaact aggtgatggt taccagctct acactatcgt tgtcttctca
120ttccccggtt atcaacatga aatacaccct ggcatgccga tgcgatgata
gttatgtgca 180acttgcaagt ccactgcagg ttaagatttt gctaaatttg
aaatgctgca aaatggatgg 240gtgcaatata tcagatatgg tatcnaaaga
ataccacaag cctatgaaca aaaagccaag 300ccattagaag cctccgtcaa
taagtggaca tcatcaagaa caatatcagc agggtcccca 360atccaaagtc
tatccctcca ccgaaacttt atcctcagct cacatactgc acaagtcccg
420tgccagattc tcgacaacta 44074201DNAZea maysmisc_feature(62)..(62)n
is a, c, g, or tmisc_feature(101)..(101)n is a, c, g, or
tmisc_feature(156)..(156)n is a, c, g, or
tmisc_feature(164)..(164)n is a, c, g, or
tmisc_feature(176)..(176)n is a, c, g, or t 74gcggcagcat gcggacgatg
gatttgagct cctccacccg atgcaccgtc gatacacgcc 60anaggttcgg ctcgccggag
tcggaaaggt ctcccgtcgt nagtacagcc gctcggtcca 120agaaccttca
cgtgggagga caataaacaa gtaacnacct ctgnaatgca catganacca
180acgtctgaag taataagcaa g 20175201DNAZea
maysmisc_feature(28)..(28)n is a, c, g, or
tmisc_feature(101)..(101)n is a, c, g, or
tmisc_feature(189)..(189)n is a, c, g, or t 75gtatgccttg gtaaggaatc
ctacatcngt gagtgattca agagtacaat tttaggaata 60aaggcgatct atatggtttc
ttttgaaaat ccattgaaag ntccgattac taaagatgtg 120aaaatggggg
agaaaagttt gtttcaagaa ttgttcaact tttactcacc cgattttaaa
180atgtaagana ttttagcttt t 20176760DNAZea maysmisc_feature(5)..(5)n
is a, c, g, or tmisc_feature(26)..(26)n is a, c, g, or
tmisc_feature(69)..(69)n is a, c, g, or tmisc_feature(209)..(209)n
is a, c, g, or tmisc_feature(522)..(522)n is a, c, g, or
tmisc_feature(587)..(589)n is a, c, g, or
tmisc_feature(614)..(620)n is a, c, g, or
tmisc_feature(622)..(625)n is a, c, g, or
tmisc_feature(635)..(635)n is a, c, g, or
tmisc_feature(639)..(639)n is a, c, g, or
tmisc_feature(641)..(644)n is a, c, g, or
tmisc_feature(648)..(650)n is a, c, g, or
tmisc_feature(653)..(653)n is a, c, g, or
tmisc_feature(655)..(659)n is a, c, g, or
tmisc_feature(668)..(670)n is a, c, g, or
tmisc_feature(672)..(673)n is a, c, g, or
tmisc_feature(677)..(687)n is a, c, g, or
tmisc_feature(690)..(691)n is a, c, g, or
tmisc_feature(693)..(700)n is a, c, g, or
tmisc_feature(703)..(703)n is a, c, g, or
tmisc_feature(707)..(711)n is a, c, g, or
tmisc_feature(716)..(726)n is a, c, g, or
tmisc_feature(729)..(738)n is a, c, g, or
tmisc_feature(740)..(758)n is a, c, g, or t 76ggccngactg ctcctgcgaa
gacgangctg acatcgagta cgacagcagc cgtatctggg 60ttctggacnc gcccaacata
ccaaagcctc caccagagac ggagaggcta gtgattatgg 120gcggtgacta
caccagaatg gacacgtatt acgtcatgcc caatgggaag cgtgcgaggt
180gtgctggtga cgtggacaag tttctggang caaatccagc gtacaaaagc
cgcatatctg 240cttcggattt cgactttgca ccgcccgagg ttgttgagga
gactgttgtt tctcacaatt 300ttctgcctct gcaaggttgc caaggccaag
aaacaggaga aggcagagag gcacacaaaa 360taggtgaaag cagagaggca
tcgatgctct gaactgtgcc tgtgccctgt gctatgtagg 420tgatagtccg
ctcaagatca cgtcacgtct cgttcctatc tagtacggtt gtctatctcg
480gctgaagtta atgtagtagg tcttcttgcg tgttaattcc tngggaatat
atgagatgtt 540taataattag ttgcttaggc cttaaaactg tgcttcctta
acttttnnnc ttcagtcatc 600tcgtatatgc acgnnnnnnn cnnnngctag
cagcntagnc nnnnactnnn cancnnnnna 660tcatagcnnn anntctnnnn
nnnnnnngcn ngnnnnnnnn ctnacannnn ntacannnnn 720nnnnnngann
nnnnnnnnan nnnnnnnnnn nnnnnnnnat 76077553DNAZea
maysmisc_feature(29)..(29)n is a, c, g, or tmisc_feature(73)..(78)n
is a, c, g, or tmisc_feature(103)..(103)n is a, c, g, or
tmisc_feature(107)..(107)n is a, c, g, or
tmisc_feature(175)..(175)n is a, c, g, or
tmisc_feature(211)..(211)n is a, c, g, or
tmisc_feature(256)..(256)n is a, c, g, or
tmisc_feature(330)..(330)n is a, c, g, or
tmisc_feature(345)..(347)n is a, c, g, or
tmisc_feature(368)..(368)n is a, c, g, or t 77tgaatgtgtc gagggcctgc
aaaatttant tttggtaaag cttaggacag aagcaaaatg 60catagatatc cannnnnnaa
atagcaaatg tttttatacg atntaancat taagtaaatc 120catttctctt
gacacaacta caggttattg tatatgagca tagcgttgat catanattct
180taccttgttt agatagttca tctgtgttac natgcacaag attacaacaa
atgagaacac 240ccatgtttgt gaatanacaa gctggttcat ccctgaaaat
gtcaccttca aggctatccc 300aagagctttg acactcatga cctggcaaan
caaagacaat acagnnngag agtatttatg 360tcctagcntt cttgagaata
gttatgtgaa ttcacatgga aagaactgtt aatcatgaga 420agcataccga
taaagatcca acaagagaac atatgccaat atataccatg atatgtgtct
480gcccatactg agggacaaaa tggcatatga gcacaaaagc tgctgcaaat
acaacagctg 540catagaatag gaa 55378201DNAZea
maysmisc_feature(101)..(101)n is a, c, g, or t 78cagtttggta
cttacttcca ctattgctga tactaagggg atgttttgta ctcttactaa 60agtttagttc
gtatcacatc gattgtttaa atgtcagcta ngaatattaa atatagttta
120attataaaac taattacaca tatgtaaaat taactagcat caccacgtta
ttcagatatt 180cctttcaatg atatcaagga t 20179201DNAZea
maysmisc_feature(101)..(101)n is a, c, g, or t 79ctttcacatt
aagagtagaa ccgaaacata aaacttcact tttatagaaa ttaattttca 60agcccgacat
ttgttcaaaa aacagcaata taaatttaag ntttttagct ccagttagat
120catcttggag gagaaacacc gtgtcatcag catattgaag acatgctacc
catctttcca 180taatgtgagc gataagacca a 20180608DNAZea
maysmisc_feature(91)..(91)n is a, c, g, or t 80tgtccgtcag
ctccgccgcc tcatccagcg tcaggttgtg gttgcacccg gtgaaggcca 60gcatgtcctt
ctcgtatctg acgacgatca nggcaaacca atgcaagttc atccattgat
120tgatctgcag ataatagccg gccaccgaac gctgtgaact ctcagctacc
tcaggtgaag 180cgcgatgtaa tgctgggact cgtttctcag ccgatcaacc
agcgtgttgc cgagctcttc 240gatctccttc ctgtactgga gcgcctcgta
gttcgcgcgg caccgaagct tttgcagcga 300aggagcgagg ccgttgttca
cgatccgtga atccgtgtgt gtaaacctca ccactttgaa 360cttcctcagg
attttcgcaa agtctctgta gaaggaagcc tggaaaacgg agctggcgat
420gcatcagcct attcagtacg tattcggtta tcctctaagc tgctgcaact
gcaatgcaat 480ccagtagagc aaacaagttg ttgactcgta ccctggacca
ggaggtgggc gctctcacgt 540acggtttcac ccttctgtaa tgtggtggga
gggaatccac gatcacaatg tcttccttca 600acgactcc 60881602DNAZea
maysmisc_feature(8)..(13)n is a, c, g, or tmisc_feature(15)..(15)n
is a, c, g, or tmisc_feature(47)..(47)n is a, c, g, or
tmisc_feature(384)..(384)n is a, c, g, or t 81aggatccnnn nnngnagcaa
tttcgaccag ggaagctcat taacagnaaa tgatgccaca 60gccagtgaaa ttgaatgtca
cagactccaa atggctgcaa taaatgatta tggatttggt 120aaacctgaga
ttccttctag ttcctcaatg ccatttttct tggctgttga tcctcaacaa
180ctgaaattga gaaatgagac aaatgtttct tcaacatctt ccaacattcc
ttcagattct 240gcatcaccaa acttgaaaaa tggcacggat cctcttttga
tgccatttaa ttcctacatg 300gcagattgga gcagcgataa gataacttac
accactctga acactccaaa aataagcaca 360gaacttccag gtcagtatgc
atcnctttct tctattatta gctaaatcaa tttagctgac 420aacaaaaact
taaccatgca gtcaagttac accatgacaa aagtagtagc tttgaagcac
480caaacctgaa ggagcatgaa tcagtctttg caacacatga aatgacggta
gaagcaacaa 540gaaaagaaga cgaacacaca tcaaaatcta gttttacttc
ctacaatgga gtaccagata 600ca 60282591DNAZea
maysmisc_feature(2)..(3)n is a, c, g, or tmisc_feature(10)..(13)n
is a, c, g, or tmisc_feature(44)..(47)n is a, c, g, or
tmisc_feature(77)..(79)n is a, c, g, or tmisc_feature(146)..(146)n
is a, c, g, or tmisc_feature(201)..(201)n is a, c, g, or
tmisc_feature(221)..(221)n is a, c, g, or
tmisc_feature(223)..(223)n is a, c, g, or
tmisc_feature(242)..(242)n is a, c, g, or
tmisc_feature(261)..(261)n is a, c, g, or
tmisc_feature(306)..(306)n is a, c, g, or
tmisc_feature(321)..(321)n is a, c, g, or
tmisc_feature(346)..(346)n is a, c, g, or
tmisc_feature(348)..(349)n is a, c, g, or
tmisc_feature(404)..(404)n is a, c, g, or
tmisc_feature(414)..(414)n is
a, c, g, or tmisc_feature(474)..(474)n is a, c, g, or
tmisc_feature(483)..(483)n is a, c, g, or
tmisc_feature(495)..(495)n is a, c, g, or
tmisc_feature(522)..(524)n is a, c, g, or
tmisc_feature(549)..(549)n is a, c, g, or
tmisc_feature(552)..(554)n is a, c, g, or
tmisc_feature(556)..(560)n is a, c, g, or
tmisc_feature(562)..(566)n is a, c, g, or
tmisc_feature(570)..(575)n is a, c, g, or
tmisc_feature(583)..(589)n is a, c, g, or t 82cnngcgcctn nnnttctaga
acaaggcgag tagcacgcct ttannnnccc ggctcgcctt 60actacctaag cgtctcnnng
gcgacgcttg aatactatgt atatactttg tttcaatagt 120taacctggta
gtttggattt tttttnaaat tctagctaac aatttgttta atacatatgc
180tgaactcttg ttaaggtgcc nttaaactgg aaggattaga nanggcaaaa
gctagtggtg 240cntgccagct tgttgtgaag natctgaaag aataatttgt
tggtgaatat acatacccct 300gcttgngtgc tggtgcaatt natgaaagaa
agtatttgct ggggantnna atggctaggc 360ctgttattgc taaggtaaag
ttgctctggt aattacattt tttnacatat aatnaccaaa 420aatgcatcct
attttcttaa aagtagatac tacttatttt cttgagatct aatngacatt
480atngcttaca gtatngttta gtagtgtgca catgcgcatg tnnnaacaca
ttattagtat 540tataattana annncnnnnn annnnntgan nnnnntgatg
tgnnnnnnng a 59183201DNAZea maysmisc_feature(4)..(4)n is a, c, g,
or tmisc_feature(15)..(16)n is a, c, g, or
tmisc_feature(101)..(101)n is a, c, g, or
tmisc_feature(180)..(180)n is a, c, g, or
tmisc_feature(192)..(192)n is a, c, g, or t 83aganacgcaa acgtnnacat
accttcatag ttcttaaggc tatatcccat gctttgatga 60cagcgccttg tccaatttca
aatgagaaaa tagagttatc ntcatgggtg gtatcgaaaa 120cttcaccagt
ctcagcaagt gtaccttcgt aatgaactat agacaaggta aaatggtaan
180tattcatatt tncatgactt t 20184561DNAZea
maysmisc_feature(140)..(144)n is a, c, g, or
tmisc_feature(147)..(148)n is a, c, g, or
tmisc_feature(253)..(253)n is a, c, g, or
tmisc_feature(274)..(274)n is a, c, g, or
tmisc_feature(335)..(335)n is a, c, g, or
tmisc_feature(437)..(438)n is a, c, g, or
tmisc_feature(462)..(462)n is a, c, g, or
tmisc_feature(474)..(474)n is a, c, g, or t 84acagaaaatt aagttataga
tatactgaca gttataccgt acaaagaagg atcttttcta 60ttaagtcaac ataaaattca
aaaatattac taaatgtcaa cataaaattc aaaaatatta 120ctaaatgtca
acataaaatn nnnnatnnta aaattcatta ctattagaag tataacaaat
180ggtgcaagga tagtgtcaat ttaaactagg caaagtatat aattgatcag
atagtagaaa 240ttcaagacgt gtntacatat gcagatcaga caanattcgg
atagtatacc acagtatgta 300cagtcaatat gcgctagtct ctacacaaac
tcaantggca gaatcaatat gcagaacttt 360gattgatgtg cattaatgaa
aaaatcattt ggctaacaat taatcataat tatgcaacaa 420attagcagag
gcaatgnntt aaccatgcca aagcatttga angacaagct accnagcaat
480atttttgttt tatcacaata aagttccaca tttacaaacc tcatcttcct
gaggacgccc 540gccatctcct ctctcttctt c 56185201DNAZea
maysmisc_feature(101)..(101)n is a, c, g, or t 85actcaccatg
atttttaggg catatatagg cactatatat acctatgcat ttcacatgac 60catccactag
ctaatatatt aattggtgac cgtccttgtt nccttacgtg ttcaagatgt
120tcctcaacat ttgcaggaaa atgtaggcac gtaggacacc gtacctttgg
agggacgacg 180gcattgcaat ttgcatgtgt a 20186201DNAZea
maysmisc_feature(4)..(4)n is a, c, g, or tmisc_feature(8)..(8)n is
a, c, g, or tmisc_feature(15)..(15)n is a, c, g, or
tmisc_feature(31)..(31)n is a, c, g, or tmisc_feature(101)..(101)n
is a, c, g, or tmisc_feature(162)..(162)n is a, c, g, or
tmisc_feature(169)..(169)n is a, c, g, or
tmisc_feature(186)..(186)n is a, c, g, or
tmisc_feature(191)..(191)n is a, c, g, or
tmisc_feature(194)..(194)n is a, c, g, or t 86aacntctngg atganatctt
tatgagacaa naggaatcga tcaaattagg gaattattaa 60tcttgtaaag aatacaacaa
acacggatat cattctaaca ncgtgccgcc agcacaacgt 120atctaaaggt
gatgccaaag gattgagaac caaaatgatt cnagacganc ccttgtggtg
180ttagangctg ntgnattagt t 20187201DNAZea maysmisc_feature(7)..(7)n
is a, c, g, or tmisc_feature(101)..(101)n is a, c, g, or
tmisc_feature(142)..(142)n is a, c, g, or t 87gtccccngta ggatccgcaa
tcctatcaac ccaccagatc ctgatcccca acaacaggta 60ctacgattct cacacgagga
tccatcttat ttggcccttt ntttgtaacg gtcgtagaca 120attatgagaa
actcaaacta cntgaccagg caggtagctg caataatgaa aaagtaaatt
180aaagatttaa taggtttgtt t 20188804DNAZea
maysmisc_feature(49)..(49)n is a, c, g, or t 88tttgcatgcc
tgcagccgcc tccttgagca catgcgagtt cacttcccng accttggcgt 60cacccaatcg
gactgcgagg agataagctg gatccagtcc accgtgtact tcgccttcta
120ctcgagctcc aagccactgg agctgctcct ggacaggagc ggcgagacgc
ccagatacgt 180caaggccaag tccgactacg tgcaagaacc catcccacgg
cacgtgtggg agagaacatg 240gtcatggctg gagaagcccg aggccgggct
gctcatcctg gacccctacg gcggccggat 300gggcagcatc tctccgtcag
cgacgccgtt cccgcaccgg aaggggaacc tgtacaacct 360ccagtactac
tcgtattggt tcgagaatgg cactgcggca ttggagaagc ggatgagctg
420ggtcaggggg ctgtacgagg agatggagcc gtatgtgtcc aagaacccaa
gaactggata 480tgtcaactac agggacctgg atcttgggac gaacgagttg
gaggacaatg tgactagcta 540cgccagggcg aggatctggg gggagaagta
tttcaaaggc aattttgaga ggctggcagc 600tgtgaaggcc atggcggatc
ctgatgactt cttcaggaat gagcagagca tccctcctct 660tcccgctgca
aaaggatggg gcttcatttg agtggtcgtt tgtttcgtag ctttgtggtg
720gtggatttct tggctacatt tgtgaattgt gaacaccggt gaggaattgc
atgggtgaag 780tgtaaaatca cttttggatt tcgg 80489434DNAZea
maysmisc_feature(223)..(223)n is a, c, g, or
tmisc_feature(297)..(297)n is a, c, g, or t 89aaagttgttt tctttgttcg
atgaactaaa taatgagtaa atttcagtga actatgtgac 60tggtcctaaa gataatttta
gcttaaccca tatccaaaac cacacgtgtg tccatattac 120cacgaactac
gctttgtaac tagaataaca acattgattt ggatgtggac tgacatactg
180ggtccattgt aaaacagttg cagctcacag actaacagtg tcntatactc
atatatgtct 240aggcccaata tgtcattgac atttgaacta acagtgttag
ctaacacgtt cattttntct 300atcatatgaa atgaacaaat attatgtagc
attgagcctt tgagaaattt gttcatattt 360ttttgataat ctagactata
tatatatcgt ttagttatta ccagtaatta ctgaagatcg 420ttcagtaatt acca
4349025DNAZea mays 90ccaacagttg ttaagaccgt tatcg 259125DNAZea mays
91actggaaatg agtccgtgtg ttatt 259225DNAZea mays 92gggtcgaaaa
gttttggttc ttgtg 259335DNAZea mays 93gcgtgattct aggtaaaagt
ttatgtttac ataat 359425DNAZea mays 94gcaacactag ataagcaact gcaat
259523DNAZea mays 95caatacggtg ggtctggata tgg 239624DNAZea mays
96cctctaaggt gaagaccgtt gtta 249722DNAZea mays 97gcaagaaggt
gatccgatca ac 229832DNAZea mays 98actactgtag aagtacaaac caattttgtg
at 329925DNAZea mays 99cgcatggcag aaacaaatac ataga 2510024DNAZea
mays 100gcagcacgca gatcaaattt acac 2410125DNAZea mays 101tcttctgagt
catttcatgg tgtgg 2510222DNAZea mays 102aaatcttgtg actcgtcctg ca
2210323DNAZea mays 103tagcagggta tgttacgcag tga 2310425DNAZea mays
104agtcttccaa ctctccttct caact 2510515DNAZea mays 105gggcgcggag
gttct 1510621DNAZea mays 106cacgggtcat tggcaatctc t 2110725DNAZea
mays 107gggaggaaaa caaatgcttg aaagg 2510822DNAZea mays
108aaaggaagct ggagctcaag ag 2210921DNAZea mays 109tggagtatgg
caacggatga c 2111019DNAZea mays 110gcgcaggact acctttgca
1911129DNAZea mays 111ggtagtatat gtgcattcat cgtttttca 2911226DNAZea
mays 112ggtttggtaa gttctgcagc taaaat 2611320DNAZea mays
113ccttgagggc accaaagaca 2011418DNAZea mays 114cggctgtgcc caaacaac
1811521DNAZea mays 115ccgcatcagt gcgtatttac g 2111619DNAZea mays
116cccaaagcgg aacgtgatg 1911721DNAZea mays 117tccagcaagc ataggcaaca
t 2111831DNAZea mays 118ggtccagtgt tttataaaga ggaataagtc a
3111926DNAZea mays 119ctctttgtac ccttgacagt gtccta 2612026DNAZea
mays 120acctatgtca tgttctacgt gctcta 2612125DNAZea mays
121agcccactga atatggaatc attag 2512221DNAZea mays 122cagcttcgcc
cagttacagt t 2112319DNAZea mays 123ctcctgaggt caccacgtt
1912427DNAZea mays 124tcagctagat ctaagtgtcg atgaaga 2712525DNAZea
mays 125caacgttctg ttcgtaagga tttca 2512621DNAZea mays
126cctcagcatt tttggcaagt g 2112722DNAZea mays 127tgaactgcaa
catgctgaaa cc 2212824DNAZea mays 128gcagttggat ttccttctgt agct
2412914DNAZea mays 129acgcgcgcgt gttg 1413022DNAZea mays
130ccaaacacgc ttgtgcaact aa 2213119DNAZea mays 131gagtctgcga
aggcatcgt 1913225DNAZea mays 132gaaaacgttt ccgatgctgt aacaa
2513324DNAZea mays 133ctgctcaagt ttgaagatgt cgtt 2413423DNAZea mays
134ttggtgggac agcacttcta act 2313525DNAZea mays 135ttctctcgtc
ttgtcaattg tcaca 2513625DNAZea mays 136ggtgtttgct atagctctgc ttcac
2513719DNAZea mays 137ccattggcat ggcgaagga 1913819DNAZea mays
138gctgggaact gtgctgatg 1913928DNAZea mays 139catggaataa tcacgtctag
tctgtctt 2814020DNAZea mays 140ccctcttttg ctccattgca 2014121DNAZea
mays 141ggctcgaagg aataccacca a 2114216DNAZea mays 142cctgacggcg
ccatct 1614319DNAZea mays 143cggagacgtc aaggatggt 1914417DNAZea
mays 144gtaggcggcg cagagtt 1714525DNAZea mays 145cgcaaggttg
ttattcagca tctct 2514623DNAZea mays 146catcgttcgg ttcatttgct tga
2314721DNAZea mays 147tggttaggcc ggatcttggt a 2114835DNAZea mays
148tgctcgtaag aaattgcaat aaataaaata aaaaa 3514924DNAZea mays
149aaccatagca gatcagcaga gtgt 2415026DNAZea mays 150gaactccaaa
gcatgagtag aaaagg 2615127DNAZea mays 151ggtttagcaa agcaatatgt
tcatggt 2715224DNAZea mays 152tctatgcaca tctcttgcct gatc
2415322DNAZea mays 153cccttgcagg attgttgttc ag 2215425DNAZea mays
154gcgtcttttt gcattattgc ctaca 2515520DNAZea mays 155ctgagttggg
aagcgctttg 2015627DNAZea mays 156tctactacat tctttagcac gctttcc
2715727DNAZea mays 157gcaatacatg atttaggact ccttgga 2715824DNAZea
mays 158gcaacttctt cgcctcatat tcag 2415938DNAZea mays 159tgaacttatt
cctatatgaa ttcttaatag aggtcctt 3816028DNAZea mays 160gtcttcccta
tcagagctta agtttctt 2816118DNAZea mays 161atccgttgcg cccagtac
1816225DNAZea mays 162aatggatggg tgcaatatat cagat 2516317DNAZea
mays 163gccggagtcg gaaaggt 1716430DNAZea mays 164gcgatctata
tggtttcttt tgaaaatcca 3016520DNAZea mays 165tgctggtgac gtggacaagt
2016626DNAZea mays 166attacaacaa atgagaacac ccatgt 2616732DNAZea
mays 167ctcttactaa agtttagttc gtatcacatc ga 3216825DNAZea mays
168tttcaagccc gacatttgtt caaaa 2516923DNAZea mays 169cagcatgtcc
ttctcgtatc tga 2317019DNAZea mays 170ccctgggaag caatttcga
1917125DNAZea mays 171ttggtgaata tacatacccc tgctt 2517225DNAZea
mays 172cgccttgtcc aatttcaaat gagaa 2517323DNAZea mays
173gcaacaaatt agcagaggca atg 2317426DNAZea mays 174catttcacat
gaccatccac tagcta 2617532DNAZea mays 175tcttgtaaag aatacaacaa
acacggatat ca 3217625DNAZea mays 176ggtactacga ttctcacacg aggat
2517718DNAZea mays 177ccgcctcctt gagcacat 1817823DNAZea mays
178gggtccattg taaaacagtt gca 2317923DNAZea mays 179cggccatcgg
aaataagcta tgt 2318021DNAZea mays 180ccagcaaagt cagacggtac t
2118119DNAZea mays 181ggccatggca tctctgaca 1918229DNAZea mays
182agtttacaaa gctagtttcc aagaaagga 2918325DNAZea mays 183caaaaaggct
gaagacggat acaaa 2518426DNAZea mays 184acccgaataa taccctcctt gactat
2618530DNAZea mays 185ttatttgagc tattctctgt tgacactgaa
3018623DNAZea mays 186agtagagacc gtggctagat cga 2318725DNAZea mays
187gtactttaca ggagcaagca ggaat 2518823DNAZea mays 188cgctgtcacc
agtatcttca gat 2318919DNAZea mays 189tgggtgcatg tgtgtgctt
1919019DNAZea mays 190ccgccgttca agaccaaac 1919122DNAZea mays
191ggcacacctt gagtccagat ct 2219224DNAZea mays 192catatgcgct
actgttccac acta 2419331DNAZea mays 193ccataacaag tgttaagtat
gagtaggact a 3119416DNAZea mays 194gctcgccgac gcagaa 1619518DNAZea
mays 195ctccagtcgc gtgaggtt 1819619DNAZea mays 196cccgcgctaa
gaactggaa 1919723DNAZea mays 197gcccgcaacc ttactgtaat ttt
2319825DNAZea mays 198tgctgtttgg tgatgttaag gtatg 2519917DNAZea
mays 199gccctctcct ccagcct 1720025DNAZea mays 200ccaaactctc
gatgaccaag cataa 2520125DNAZea mays 201gcggaagttt gaaatactgc tgatt
2520223DNAZea mays 202gctctcttgg atgttggcct tat 2320323DNAZea mays
203gattttgctc catgctagtt tcg 2320419DNAZea mays 204cgtgcgacgt
cctgatacc 1920519DNAZea mays 205ctgccacagc cggaatttg 1920625DNAZea
mays 206gaagatgtcc cgatagcgaa ttgta 2520724DNAZea mays
207tgcaaatcat gtgacgtacc attg 2420821DNAZea mays 208ctctctcgcc
ccagaagaac t 2120921DNAZea mays 209ccgagtatgc atctggactg t
2121029DNAZea mays 210gtctatatat gtaaccaccc tctatgcaa 2921124DNAZea
mays 211gtcatctggg gactacactt tgtg 2421225DNAZea mays 212gacgaatact
cttacgaggc aagtt 2521329DNAZea mays 213gaaccactct attctaattg
actctccaa 2921428DNAZea mays 214gaataacagg accatatctg aagatcga
2821530DNAZea mays 215gtattttttt tcttttttag acgttgcttt
3021626DNAZea mays 216gctttttgaa ccatgtatca gagaaa 2621720DNAZea
mays 217ggaagcaaca gcaggctgtt 2021821DNAZea mays 218agcctggtgt
acgagaatgt g 2121927DNAZea mays 219gttggtagga ttggaactta ggttaca
2722024DNAZea mays 220aagatcagca agcagagttg agat 2422121DNAZea mays
221gttgttgctc
gtttgcatcg a 2122231DNAZea mays 222gtttctagat gactttagac attcagagtg
g 3122322DNAZea mays 223gattcagttc acctcgcaac ct 2222422DNAZea mays
224ttcccctgat cgagagtagc ta 2222525DNAZea mays 225ggtctagaaa
aacagcttca aatgc 2522622DNAZea mays 226agaaagttcg cagattgcca ga
2222718DNAZea mays 227gttggcctcc aggaacct 1822829DNAZea mays
228atatgttctt cttgctattg tgctattca 2922921DNAZea mays 229cttcgctggt
acatgccttc t 2123019DNAZea mays 230tgctgtacgc cgtttcgtt
1923116DNAZea mays 231aggtggcgcc agtgga 1623217DNAZea mays
232cccgcctgct ccatctc 1723319DNAZea mays 233gcaacgagcc gatggaatc
1923420DNAZea mays 234cgatgccgtt cactcaggaa 2023522DNAZea mays
235cgccttggta ctcgtagatt gc 2223625DNAZea mays 236tgtgtttgat
acatggcctt cagat 2523717DNAZea mays 237gccgaccagg ccatttc
1723821DNAZea mays 238gccaaccctt ctggtcttct c 2123925DNAZea mays
239cgacagaaat gattgagtca caaca 2524024DNAZea mays 240gccaccacta
agaactagag tcaa 2424121DNAZea mays 241tgctggattc tgaaccacga t
2124225DNAZea mays 242ggactgcact cacatgttat ttcct 2524323DNAZea
mays 243gatatcacgt acgtgctgca ttc 2324426DNAZea mays 244gtctaatgag
caggttatgc tgatct 2624525DNAZea mays 245agacttcagg aatgagggag tatca
2524625DNAZea mays 246ctctcgaatg cacttgcttt gttaa 2524726DNAZea
mays 247acagggtgac attagtgtag tgtttg 2624823DNAZea mays
248gtatgctaca gtccctccct cta 2324922DNAZea mays 249tccccaatcc
aaacacaacc tt 2225021DNAZea mays 250tgccagtgtc tgtgagctca a
2125122DNAZea mays 251ggcttctaat ggcttggctt tt 2225220DNAZea mays
252cccacgtgaa ggttcttgga 2025325DNAZea mays 253caaacttttc
tcccccattt tcaca 2525420DNAZea mays 254cgaagcagat atgcggcttt
2025523DNAZea mays 255gaaggtgaca ttttcaggga tga 2325634DNAZea mays
256gtggtgatgc tagttaattt tacatatgtg taat 3425724DNAZea mays
257ggtgtttctc ctccaagatg atct 2425819DNAZea mays 258cgcttcacct
gaggtagct 1925925DNAZea mays 259ttggagtctg tgacattcaa tttca
2526024DNAZea mays 260ttagcaataa caggcctagc catt 2426125DNAZea mays
261gctgagactg gtgaagtttt cgata 2526227DNAZea mays 262agatgaggtt
tgtaaatgtg gaacttt 2726322DNAZea mays 263cgtgcctaca ttttcctgca aa
2226425DNAZea mays 264tggcatcacc tttagatacg ttgtg 2526518DNAZea
mays 265cagctacctg cctggtca 1826618DNAZea mays 266tcctcgcagt
ccgattgg 1826724DNAZea mays 267tcaatgacat attgggccta gaca
2426816DNAZea mays 268tcgacaacca aaaggt 1626914DNAZea mays
269caaggcggca acgc 1427020DNAZea mays 270ctaatttaga cgtcacccgc
2027124DNAZea mays 271aactgaatta ttgtatatac cttc 2427220DNAZea mays
272agtgagcttg ttacttctta 2027317DNAZea mays 273cagaccctga tcctgaa
1727420DNAZea mays 274ttcaaataag aagaaagttc 2027513DNAZea mays
275cagaggcatg gcc 1327620DNAZea mays 276tttgttctta tgaatttcgt
2027720DNAZea mays 277ccttccagta tctttttagt 2027816DNAZea mays
278tcaaaacgca ggccaa 1627920DNAZea mays 279cagttgattc atttaatctg
2028016DNAZea mays 280tcgaaggaca acacaa 1628116DNAZea mays
281tgggaggatt ctaatg 1628220DNAZea mays 282catagtacac agtacactcg
2028316DNAZea mays 283atctcgacgc cggtct 1628418DNAZea mays
284caatctctgg acagatac 1828518DNAZea mays 285cagtttcgtc gccgtcag
1828617DNAZea mays 286atcatcgatc gacatcg 1728715DNAZea mays
287accttgctgg ataat 1528815DNAZea mays 288tgcttcaaga atcag
1528918DNAZea mays 289cttgattaga ccaaagtg 1829017DNAZea mays
290caggtcaatg aaggagt 1729114DNAZea mays 291cccagtcgta gtcc
1429217DNAZea mays 292cttctctgtt gaataga 1729316DNAZea mays
293aactgcggct gtgtac 1629420DNAZea mays 294ctgtagttcc attcgttgta
2029516DNAZea mays 295tgtcaacaga ttcgac 1629617DNAZea mays
296ctaaggcaca ggtagag 1729716DNAZea mays 297actgtagtgc catttt
1629814DNAZea mays 298ccggaccatt cgac 1429916DNAZea mays
299actgatattc aaattc 1630014DNAZea mays 300tgggcaaggt acgc
1430116DNAZea mays 301catgaatcag ctttgc 1630218DNAZea mays
302ccaaacacac ctaagtat 1830320DNAZea mays 303cattttccga gtaactcatt
2030414DNAZea mays 304tgtggagagg aacg 1430514DNAZea mays
305ccaaccacaa ttta 1430616DNAZea mays 306catggagtca ctagga
1630717DNAZea mays 307tcgtgtcatc ggagcgt 1730818DNAZea mays
308caacacttag ataaaacc 1830915DNAZea mays 309cgatggaaat ccctg
1531019DNAZea mays 310aaatcacgtt gtatctatc 1931116DNAZea mays
311ctgttttcgt aaattc 1631218DNAZea mays 312attagaatga cataactc
1831316DNAZea mays 313atcgccgatg gagcta 1631415DNAZea mays
314ctggaataga tctgc 1531516DNAZea mays 315cctacttgca tccatc
1631614DNAZea mays 316ccgaggtaac atac 1431715DNAZea mays
317cttggacaag caaaa 1531815DNAZea mays 318agacgatcac ggcgg
1531915DNAZea mays 319accaggacct tgaaa 1532014DNAZea mays
320tgtcccgaga cctg 1432117DNAZea mays 321ccaagaagca cgtcgac
1732214DNAZea mays 322caacgacgag accc 1432314DNAZea mays
323cagcacgaac tcga 1432416DNAZea mays 324tccaggaaga tgattg
1632518DNAZea mays 325cactaacaac cagatttg 1832620DNAZea mays
326caagaacaat gaagcatagt 2032715DNAZea mays 327aggaggctta aatga
1532820DNAZea mays 328aaagagaaat caacagtttg 2032921DNAZea mays
329tttggcgtac atattgatat a 2133014DNAZea mays 330cctttcgcct tctg
1433118DNAZea mays 331cggtacaagt agaatcta 1833218DNAZea mays
332cagaagacac ggtgatta 1833317DNAZea mays 333tgccaccctg aacacta
1733416DNAZea mays 334accacagtag gaatgc 1633516DNAZea mays
335tccgacaagt gggaac 1633622DNAZea mays 336tgacaaaatt tgaaaatcta gc
2233716DNAZea mays 337cagctctcct ttgtcg 1633819DNAZea mays
338tgatcgtgct aatagaatt 1933915DNAZea mays 339agcagatgtg atccg
1534017DNAZea mays 340tggtatcaaa agaatac 1734116DNAZea mays
341cccgtcgtca gtacag 1634219DNAZea mays 342ttagtaatcg gaactttca
1934313DNAZea mays 343ctggacgcaa atc 1334415DNAZea mays
344ccagcttgtt tattc 1534518DNAZea mays 345atgtcagcta tgaatatt
1834620DNAZea mays 346ctggagctaa aaaacttaaa 2034715DNAZea mays
347acgatcaagg caaac 1534816DNAZea mays 348ctcattaaca gaaaat
1634917DNAZea mays 349tctttcatga attgcac 1735016DNAZea mays
350cacccatgaa gataac 1635115DNAZea mays 351ctaccaagca atatt
1535216DNAZea mays 352cacgtaaggg aacaag 1635315DNAZea mays
353cggcacggtg ttaga 1535419DNAZea mays 354atttggccct ttttttgta
1935514DNAZea mays 355ttccccgacc ttgg 1435618DNAZea mays
356ctaacagtgt catatact 1835715DNAZea mays 357cgacaaccga aaggt
1535814DNAZea mays 358caaggccgca acgc 1435916DNAZea mays
359tttagacgcc acccgc 1636022DNAZea mays 360ctgaattatt gtatatgcct tc
2236118DNAZea mays 361tgagcttgtt gcttctta 1836217DNAZea mays
362cagaccctga ccctgaa 1736321DNAZea mays 363cttcaaataa gaataaagtt c
2136414DNAZea mays 364aggcagagac atgg 1436520DNAZea mays
365tttgttctta tgattttcgt 2036620DNAZea mays 366ccttccagta
tctctttagt 2036715DNAZea mays 367caaaacgccg gccaa 1536820DNAZea
mays 368cagttgattc atttgatctg 2036914DNAZea mays 369cgaagggcaa caca
1437015DNAZea mays 370tgggagggtt ctaat 1537118DNAZea mays
371tagtacacag tgcactcg 1837217DNAZea mays 372catctcgaca ccggtct
1737316DNAZea mays 373atctctggcc agatac 1637418DNAZea mays
374cagtttcgtc accgtcag 1837515DNAZea mays 375catcgatcaa catcg
1537615DNAZea mays 376ttgctgtata atttc 1537716DNAZea mays
377ttgcttcaag gatcag 1637816DNAZea mays 378tgattaggcc aaagtg
1637917DNAZea mays 379caggtcaatg gaggagt 1738016DNAZea mays
380cccagttgta gtccta 1638118DNAZea mays 381atgcttctct gttaaata
1838214DNAZea mays 382ctgcggcagt gtac 1438320DNAZea mays
383ctgtagttcc attcattgta 2038416DNAZea mays 384tgtcaacaaa ttcgac
1638517DNAZea mays 385ctaaggcaca agtagag 1738616DNAZea mays
386actgtagtgc cgtttt 1638714DNAZea mays 387ccggaccgtt cgac
1438817DNAZea mays 388aactgatatt caacttc 1738913DNAZea mays
389tgggcagggt acg 1339016DNAZea mays 390catgaatcaa ctttgc
1639117DNAZea mays 391caaacacacc caagtat 1739220DNAZea mays
392cattttccga gtacctcatt 2039314DNAZea mays 393tggagtggaa cgct
1439414DNAZea mays 394ccaaccacaa ctta 1439514DNAZea mays
395tgcatggaat cact 1439616DNAZea mays 396cgtgtcatca gagcgt
1639716DNAZea mays 397acacttagac aaaacc 1639815DNAZea mays
398cgatggaatt ccctg 1539918DNAZea mays 399aatcacgttg tttctatc
1840017DNAZea mays 400tctgttttct taaattc 1740117DNAZea mays
401ttagaatgac acaactc 1740216DNAZea mays 402atcgccgatt gagcta
1640315DNAZea mays 403ctggaatagc tctgc 1540418DNAZea mays
404aacctacttg tatccatc 1840514DNAZea mays 405ccgaggtgac atac
1440615DNAZea mays 406cttggacatg caaaa 1540714DNAZea mays
407agacggtcac ggcg 1440816DNAZea mays 408ccaggatctt gaaaca
1640913DNAZea mays 409tcccgcgacc tga 1341017DNAZea mays
410ccaagaaaca cgtcgac 1741114DNAZea mays 411caacgaccag accc
1441214DNAZea mays 412cagcacaaac tcga 1441316DNAZea mays
413tccaggaagc tgattg 1641417DNAZea mays 414actaacaacc ggatttg
1741520DNAZea mays 415caagaacaat gaaacatagt 2041614DNAZea mays
416aggctgaaat gaat 1441719DNAZea mays 417aagagaaatc aacggtttg
1941819DNAZea mays 418tggcgtacat atcgatata 1941914DNAZea mays
419ccccttttgc cttc 1442018DNAZea mays 420cggtacaagt aaaatcta
1842118DNAZea mays 421cagaagacac gttgatta
1842217DNAZea mays 422tgccacccta aacacta 1742315DNAZea mays
423ccacagtaag aatgc 1542416DNAZea mays 424tccgacaaat gggaac
1642520DNAZea mays 425acaaaatttg aacatctagc 2042615DNAZea mays
426agctctccgt tgtcg 1542719DNAZea mays 427tgatcgtgct attagaatt
1942814DNAZea mays 428agcagacgtg atcc 1442916DNAZea mays
429atggtatcga aagaat 1643017DNAZea mays 430tcccgtcgtt agtacag
1743114DNAZea mays 431aatcggagct ttca 1443213DNAZea mays
432ctggaggcaa atc 1343316DNAZea mays 433cagcttgtct attcac
1643415DNAZea mays 434tcagctacga atatt 1543520DNAZea mays
435ctggagctaa aaatcttaaa 2043614DNAZea mays 436cgatcagggc aaac
1443718DNAZea mays 437attaacagga aatgatgc 1843817DNAZea mays
438ctttctttca taaattg 1743915DNAZea mays 439acccatgagg ataac
1544015DNAZea mays 440agctaccgag caata 1544115DNAZea mays
441acgtaaggaa acaag 1544215DNAZea mays 442cggcacgatg ttaga
1544318DNAZea mays 443tttggccctt tctttgta 1844414DNAZea mays
444ttcccggacc ttgg 1444516DNAZea mays 445acagtgtcgt atactc
16446201DNAZea maysmisc_feature(11)..(11)n is a, c, g, or
tmisc_feature(39)..(39)n is a, c, g, or tmisc_feature(44)..(44)n is
a, c, g, or tmisc_feature(101)..(101)n is a, c, g, or
tmisc_feature(177)..(177)n is a, c, g, or t 446cactagaagt
ngggtatatg attggaacta aagacactng tccngtcata atatgggatc 60atatcatcca
caaaaaatga acaagaagtg tccagataaa ncaaagctca aaggtttaat
120ccattagact atgtgcttgc catggagaat gagttgcatg atgaatccaa
cagtganagt 180gatggggagc ttcatttgtt g 201447201DNAZea
maysmisc_feature(19)..(19)n is a, c, g, or tmisc_feature(67)..(67)n
is a, c, g, or tmisc_feature(70)..(70)n is a, c, g, or
tmisc_feature(101)..(101)n is a, c, g, or t 447ttgatacaca
aatatgatnt ttcatatttg cgtagaatat agtgcctttg attgagcctc 60actatantan
cccatggatg atcggatgat gttagaattt ntgtagtggt atttagatgg
120acatacttga catttggatg gatgaatgca ctagatatat atttggattg
atggaataga 180tggtattaga acttgaatta t 201448675DNAZea
maysmisc_feature(8)..(8)n is a, c, g, or tmisc_feature(17)..(19)n
is a, c, g, or tmisc_feature(25)..(25)n is a, c, g, or
tmisc_feature(72)..(76)n is a, c, g, or tmisc_feature(216)..(216)n
is a, c, g, or tmisc_feature(532)..(534)n is a, c, g, or
tmisc_feature(547)..(547)n is a, c, g, or
tmisc_feature(573)..(573)n is a, c, g, or
tmisc_feature(575)..(575)n is a, c, g, or
tmisc_feature(578)..(580)n is a, c, g, or
tmisc_feature(597)..(602)n is a, c, g, or
tmisc_feature(605)..(605)n is a, c, g, or
tmisc_feature(607)..(623)n is a, c, g, or
tmisc_feature(630)..(630)n is a, c, g, or
tmisc_feature(646)..(674)n is a, c, g, or t 448tggttcangt
caaatcnnna aactngtttt gtgacaattg gacaacgagt gcttgccaat 60ccactgaggt
annnnncttg gttcatattt cattttacat tcatgttgtg gttatgcatt
120atctattggt tctagtattt caatctttag gttgtgtgaa tcgtcctctt
atttgttttg 180ttcttcgggg tgttactatc ctaagatgtt tgcttngtaa
tttttttcct gaaccttctt 240tattcagggt tcggtttcat tatggccatc
ctgatgtctt cgatcgtctt ttccacgtta 300cgaggggcgg tgtcagtaaa
gcatccaaaa ttatcaatct tagtgaggac atctttgcag 360gtacttttct
tttgctgtta ctgctcaaca tttgtaaatg tggaaccact aagataatat
420tttggcttct tatggttcag gattcaattc cacattgcgc gaaggcaatg
ttactcacca 480tgaatacatg caagttggca aaggaaggga tgtaggtctc
aatcaaatat cnnngtttga 540ggcaaanata gcaaatggca atggcgaaca
aangntgnnn cgtgacatct accggcnnnn 600nnatngnnnn nnnnnnnnnn
nnntgctgtn ttgttactac acaacnnnnn nnnnnnnnnn 660nnnnnnnnnn nnnnt
675449667DNAZea maysmisc_feature(184)..(184)n is a, c, g, or
tmisc_feature(566)..(573)n is a, c, g, or
tmisc_feature(622)..(624)n is a, c, g, or
tmisc_feature(641)..(645)n is a, c, g, or
tmisc_feature(648)..(648)n is a, c, g, or
tmisc_feature(655)..(661)n is a, c, g, or t 449tccacacgtt
cacgacctgt accttgacac ctttcccatt ctatctacga gtaaacaggg 60cttcttatgg
atgctggagc aacccaagga tagcagcatc aatggcgcta gcccagggat
120cagagacagg aagctcctcg tcgaggtttt ccctgtaaaa atgtctgcta
cgcttcaagg 180acantccctc accttgtctg gacccgatga tgcctcgcag
ctaaccattg acctgctcgg 240ttgcacagtt gctgctgttt ctgcatccaa
tctgccctca cgtaaatggt tagtgcaaat 300cgtagctggc ctcttcaaca
ttcatctctc tctacttatt gttgttgttt tcttgccatc 360agctacaaag
cgtgacttac tcggtgtatg cattattatg cttttagcag ctatctggat
420ccttgactta ctacataata caatacccag atttgataca ctcctactat
ataagaaatc 480agagtcaaat aactgtagca ggctcctaaa gttgattata
cagtttggct aaaactaccg 540tcagtacctg tttttttttc agggcnnnnn
nnnatccgat aaaactggaa agcaaggaat 600ctggtatttg cagggggagc
annntatgct atgtttatgc nnnnnccnct tgggnnnnnn 660natcatg
667450201DNAZea maysmisc_feature(2)..(2)n is a, c, g, or
tmisc_feature(29)..(29)n is a, c, g, or tmisc_feature(101)..(101)n
is a, c, g, or tmisc_feature(125)..(125)n is a, c, g, or
tmisc_feature(140)..(140)n is a, c, g, or t 450tntgcacctg
caggcacctg agcggctcnt accatggctt gtgcgacacc tcctacaccc 60cctgcctaga
tacatgcaaa ggggaagaca gcaacaacgt nggcggcgct tgctttgact
120ccccncctcg ttgctggtgn ttcaccaact gctagctgag ctcgccgtga
tgtcgtcgat 180cccatcctct tactcggcct c 201451201DNAZea
maysmisc_feature(76)..(76)n is a, c, g, or
tmisc_feature(101)..(101)n is a, c, g, or
tmisc_feature(154)..(154)n is a, c, g, or
tmisc_feature(156)..(156)n is a, c, g, or
tmisc_feature(175)..(175)n is a, c, g, or t 451tgtaattgca
gttgtctctc tctttttggc tcaatttgcc cttttgtttt gttgctttgc 60cctcatgtaa
tcgtanatga tatttgttgg atgttctaac nactggcata gattgaagtg
120tgctgaaata aattcctgct ggtgagaaac agcncncttc gtgtcagtta
actcngagat 180ttatcataga tgagatgaat t 201452492DNAZea
maysmisc_feature(215)..(215)n is a, c, g, or
tmisc_feature(265)..(265)n is a, c, g, or t 452ttggaattga
catcagagaa gacgattgag gaactttctc aatggtgacc ttgcaatgct 60cgttcctgcc
aatgattggg tatgccaaat gatttccggt ggctgtagaa ggtgatgttg
120agcaagaagg aatttcctcc gttagtacag attgtgtagc acctggagtc
atcagttggg 180ttccagcttt ggacaaaacg ttggttgatg aaatntgtgg
atagacaaca tctgacacat 240ctgcaagcac aaattgtttc tgtgnaggct
tctgctgttc ttgcttcatt ggtggtggcg 300cctgtgatgc ttgcagcgaa
acatcgctat cttgttgagg caggatccgc tgctgggcaa 360gtgaatgaga
gcttgatagc tgctgcatgt ccaaaagtaa cttctgttgt tcctgtatta
420cctgtaacgg cgagagtgta actgttggct gtgatacaag tgactgctgc
tgtagtttct 480gtaatagctg ca 492453201DNAZea
maysmisc_feature(1)..(1)n is a, c, g, or tmisc_feature(11)..(11)n
is a, c, g, or tmisc_feature(101)..(101)n is a, c, g, or
tmisc_feature(170)..(170)n is a, c, g, or
tmisc_feature(196)..(196)n is a, c, g, or t 453ncaattattt
nggacctcta ggcctcacta tacatggatg agatgttcaa cattgacctt 60tccaacaatt
ctattgatgg agctttctca acacttaggg ntagtttgtg aactctattt
120tcccaaagga ttctcatttt cccaagagaa aatgaactaa tttcccttgn
aaaaataggg 180ttgccaaact agcccntaca a 201454789DNAZea
maysmisc_feature(4)..(9)n is a, c, g, or tmisc_feature(93)..(93)n
is a, c, g, or tmisc_feature(99)..(99)n is a, c, g, or
tmisc_feature(105)..(105)n is a, c, g, or
tmisc_feature(157)..(157)n is a, c, g, or
tmisc_feature(161)..(161)n is a, c, g, or
tmisc_feature(283)..(283)n is a, c, g, or
tmisc_feature(298)..(298)n is a, c, g, or
tmisc_feature(331)..(331)n is a, c, g, or
tmisc_feature(341)..(341)n is a, c, g, or
tmisc_feature(345)..(345)n is a, c, g, or
tmisc_feature(347)..(347)n is a, c, g, or
tmisc_feature(367)..(368)n is a, c, g, or
tmisc_feature(370)..(370)n is a, c, g, or
tmisc_feature(391)..(391)n is a, c, g, or
tmisc_feature(452)..(452)n is a, c, g, or
tmisc_feature(461)..(487)n is a, c, g, or
tmisc_feature(492)..(500)n is a, c, g, or
tmisc_feature(523)..(523)n is a, c, g, or
tmisc_feature(537)..(539)n is a, c, g, or
tmisc_feature(547)..(547)n is a, c, g, or
tmisc_feature(580)..(580)n is a, c, g, or
tmisc_feature(587)..(587)n is a, c, g, or
tmisc_feature(670)..(670)n is a, c, g, or
tmisc_feature(686)..(690)n is a, c, g, or
tmisc_feature(726)..(727)n is a, c, g, or
tmisc_feature(735)..(735)n is a, c, g, or
tmisc_feature(745)..(745)n is a, c, g, or
tmisc_feature(747)..(753)n is a, c, g, or
tmisc_feature(755)..(755)n is a, c, g, or
tmisc_feature(757)..(782)n is a, c, g, or
tmisc_feature(784)..(788)n is a, c, g, or t 454cttnnnnnnt
caggttcgtt cttctcttcc tccttgtaga atctaacatt ctctaggagc 60agaactccac
cttctggcaa agcggcagcc aanttctcna cttcntcacc aatgcaatca
120ttggccatca cgacctattt atgcacacat attagcnctt ngaataactg
aaagatagca 180ggtactaaaa taagctaatg tcaaatctta gcatacttca
actccaagga gctcagacaa 240gcgtggaaca agaggcttca agctgtactt
tggggtgaca ccntttggac gaccctgnaa 300atggcatcaa caaattgcat
tattacaaaa ntaaaagaca naaanantta ttgttgctac 360actcaanncn
aaaaaggttg acttgttact nttagtaacc aaaaagattg attcacaaag
420cacaagcaca tacaggttaa gcaaccacag anaatcagtt nnnnnnnnnn
nnnnnnnnnn 480nnnnnnntga annnnnnnnn tatttccaaa aacagtacac
ttngctataa attatcnnng 540tgcgaantgc cgattgccaa ggggcacaaa
atctctcacn aatatgntgt ttaagagcat 600cagctatcag gttctagata
taaaatcatt tcatatcatg gaaaaaaacg ctagtattat 660tttcatactn
tcaaacaaaa tgaaannnnn gttgcagcag acgataggat ctgacttgga
720aatgannatg atagnctgaa ctgantnnnn nnntngnnnn nnnnnnnnnn
nnnnnnnnnn 780nntnnnnnc 789455759DNAZea maysmisc_feature(18)..(19)n
is a, c, g, or tmisc_feature(106)..(106)n is a, c, g, or
tmisc_feature(161)..(161)n is a, c, g, or
tmisc_feature(174)..(174)n is a, c, g, or
tmisc_feature(254)..(254)n is a, c, g, or
tmisc_feature(280)..(280)n is a, c, g, or
tmisc_feature(302)..(302)n is a, c, g, or
tmisc_feature(368)..(368)n is a, c, g, or
tmisc_feature(410)..(410)n is a, c, g, or
tmisc_feature(503)..(503)n is a, c, g, or
tmisc_feature(552)..(552)n is a, c, g, or
tmisc_feature(564)..(564)n is a, c, g, or
tmisc_feature(595)..(605)n is a, c, g, or
tmisc_feature(608)..(612)n is a, c, g, or
tmisc_feature(615)..(624)n is a, c, g, or
tmisc_feature(663)..(663)n is a, c, g, or
tmisc_feature(741)..(741)n is a, c, g, or
tmisc_feature(755)..(756)n is a, c, g, or
tmisc_feature(758)..(758)n is a, c, g, or t 455ctgctgttgc
cggcttcnnc atgcagaagg taataaaatg tcacccatgc tcatgatctt 60ttaagtgtat
ttaggtactg ttaagtagga tggggtaaat attttntacc tttcaaaaga
120gaccaataaa tctttttaac atttcagttt tttcataaat naaaaaagga
aagntttgac 180aaatgacttt attatctcag gaacttgact accttgttgg
agctgttgcc aacccaaaga 240agccatttgc cgcnattgtt ggtggatcca
aggtctcaan taagattggt gtcattgagt 300cnttgctggc gaaggtcgat
atcctcatcc ttggtggtgg tatgatctac acattttaca 360aggcacangg
atattctgtt ggaaaatctc tcgtggaaga ggataaactn gagctcgcaa
420cttctcttat tgagaaggcg aaggcaaagg gggtttctct tttgcttccc
actgatattg 480tagtagcgga caagtttgca gcngatgctg agagcaaggt
ttgtttattt acacataaac 540acctcagatg gnacttcaga attntttctt
tctttccctt taatgaatcg tgaannnnnn 600nnnnnagnnn nnctnnnnnn
nnnnattcac tagtaattct gccgcataag tcgtgcagca 660gantagaatt
aggcatgaat agttcagtgt taatagtgat aagacatgtt attttgtatg
720ataggttttc cacagtgcag naccggaaac aaatnngnc 759456577DNAZea
maysmisc_feature(322)..(322)n is a, c, g, or t 456tgctaagatt
tgacattagc ttattttagt acctgctatc tttcagttat tccaagtgct 60aatatgtgtg
cataaatagg tcgtgatggc caatgattgc attggtgaag aagttgagaa
120gttggctgcc gctttgccag aaggtggagt tctgctccta gagaatgtta
gattctacaa 180ggaggaagag aagaacgaac ctgagtttgc taagaagcta
gcatctgttg ctgaccttta 240tgtcaatgat gcttttggca cggcacacag
agctcatgct tcaaccgaag gagttaccaa 300gtatttgaag cctgctgttg
cnggcttcct catgcagaag gtaataaaat gtcacccatg 360ctcatgatct
tttaagtgta tttaggtact gttaagtagg atggggtaaa tatttttgta
420cctttcaaaa gagaccaata aatcttttta acatttcagt tttttcataa
ataaaaaaag 480gaaaggtttg acaaatgact ttattatctc aggaacttga
ctaccttgtt ggagctgttg 540ccaacccaaa gaagccattt gccgctattg ttggtgg
577457500DNAZea maysmisc_feature(345)..(345)n is a, c, g, or
tmisc_feature(480)..(480)n is a, c, g, or t 457ttccaaattc
ttttccaggg ctcaaaggat tccgctccaa tgaaaaaagc tctgtaggca 60tctactagac
agaatactca aaacagtctt gcgtttcctt atatcgacat ctatcttact
120aatgtttaca gggagctatc cttgatattt tagaacagtt ttactgtaat
ttgaaagcag 180caattacctt attcatttat accaaactgc caataaggtc
cattgtcaac tgatggatag 240agtatttaag gctttactta tttctggaca
aagtgttttg ttactataac aaaagtaacc 300agtgaggaat ttgcatttaa
ttttctgtcc aacgcttatt atgcntgcaa ctctatgttt 360atatagcacc
aaaccaccaa tgttaattga agaattaata tcgattctaa gtggatgcct
420gtttcctcat ttactttatt cattacaaac gttgattgaa gaatttctgt
aaatactgan 480tttttattcc cttttctgtg 500458412DNAZea
maysmisc_feature(58)..(58)n is a, c, g, or t 458ccaaggactg
acggaacgga tcggatggat tgggcgattg gcatcgcagg agattctnga 60tcagattgtc
ttcgagcgca cgccgcaatg gtcaactgcg cagcgctggc ttggctgctg
120cctccgttca ctcttcgctg tcagagcgaa acagcggtaa cagagcaaga
cacgaggggt 180ctgcactctg cagttgtgca ggaggaggaa cagacacagg
gagaggaggg cgcgcggccg 240gacatggcgt ggaacagaga tcgtcgtcac
cgccggcggg ggtgtggtcg acgacgcgac 300atgcgaaaca ctacgtgcta
gctagcatag gaaaaaacaa catgaaatcg tacaaccaac 360caatccctcg
cactgtttta gttgttagct gtaggcgaaa ctgttcatcc aa 412459664DNAZea
maysmisc_feature(342)..(342)n is a, c, g, or
tmisc_feature(609)..(613)n is a, c, g, or
tmisc_feature(627)..(627)n is a, c, g, or
tmisc_feature(642)..(656)n is a, c, g, or
tmisc_feature(662)..(663)n is a, c, g, or t 459tgtggcacag
ccggtctgga aggagaccgc agcaatagtc gatttgaggg catatagcag 60tgaagaaaaa
gcaggaggga gaagttttgg gtggtggcca tgcatctgga taatgggaga
120agcaaggtgc gccaagggag agagcattca cacaagagac agaggacgag
cagtggcgct 180gggaatctgg gtccccatta aatgtatcca tgtaggttac
ccaggggagg gtacatgggc 240tggctgatgg gctgggccag ctccttctta
ctcctccctt ctcctttttt cttttttccc 300tttttcctca gccacatgtt
agaatttgta tggcactatg gngtagcctt tcctcgcctt 360atcaccgcct
aggcatcaca tagacatttg ctaagtgttt ggttcgccga aatgtaatgt
420aaatggatta cacgaggtaa tggataccga tacttatgtt tggttgagtt
ctttctagta 480atactaggta ctaatatcca atccaattat aactagtgac
cattaccgcc ggtaagggat 540cccattacca ttcccttaac aatatatgaa
ccaaacatca ccttggaaga agctggctcg 600ctacaactnn nnnatagcac
cttaagnacc atgattaaaa tnnnnnnnnn nnnnnncaca 660tnnc
6644601203DNAZea maysmisc_feature(234)..(258)n is a, c, g, or
tmisc_feature(542)..(542)n is a, c, g, or t 460ggccgggatg
tcagagaaag gaaaacccac accggccaaa ccacgactgc aaagagaaag 60gaaaggctct
ctcccagccc caaagaaaaa ggcagcacac caactcacca agaaagtgtg
120gagctgctcc tgcctgcagc ctcctgctca tcgacccttt cgccccacaa
gctcgattcc 180tcgctcggcc atggctgcac ccgcggagga gacgcttgcc
gccgaggata cggnnnnnnn 240nnnnnnnnnn nnnnnnnntg cggggttctg
gttccttggc gaggataagt ctgtccacaa 300ggctctcggc gggggtaaaa
ctgctgatgt acttttgtgg aaagacaaga agacctctgc 360tgcggtagtt
ggtggtgcaa ctgtcctatg ggttctgttc gaaattgtag aataccatct
420cctgaccctg gtttctcatg tgctgatcgc tgcactgacc atcttgttcc
tgtggtccaa 480tgcgactgtc tttatcaaaa agagtcctcc tgatgttcct
gaagtgcaga tatctgaaga 540cnttgctgta aacattgcgc tagcattgcg
tgctgacatc aacaaggcac ttgccctgct 600tcgggagatt gctctgggcc
ataacctgat gaagttccta ggcgtggtcg tcgccctttg 660gattctttca
gagatcgggg agctatgtga tctcctgaga ttgatgtaca ttgtggtctt
720gatcctccac acagtgccga tactgtacca caagtatcag gaccaggtgg
acaatttcgc 780tgcaaaggcg cacagggagc tctgcaagca atacgcggtc
ctggatgcca aagttctgag 840caagattcca agagctccac caaaagataa
gaaacagaac tagaacgtgg aggatgacaa 900gcgccttgtt ttcggcttgg
gcgacgagag tttgtagcca atggtctgat gtggttcgtg 960ggctttgtgt
aggtgcacag tttcacgtat atgttctggt cgtgtaatct atatgtttat
1020ataaacctgt aggggtgttg atgaccatcg aaagtgtcgt aaatattcag
tagtatatgt 1080agcttagggc ttaacggttg tgtttataag gtcattgtcg
ttgtcgatgg gaagtattat 1140catgttattt tcaggactca ggagtataaa
atgaaataaa atgtgaattc atctgaaaaa 1200aaa 1203461201DNAZea
maysmisc_feature(101)..(101)n is a, c, g, or
tmisc_feature(132)..(132)n is a, c, g, or t 461cttgtcatga
tatatttttt tcttcaaatg tgtttgagtt ttttctctag aatatttact 60attacaatga
ctcaacccat ctttctctgt agttggtgac ncttttgtta ctgaggacac
120tacaaatgtg angtttcctc gggaagtaac agttccaggc tatacacacc
cattggttgc 180agttggcaca ggtatttttc a 201462262DNAZea
maysmisc_feature(73)..(73)n is a, c, g, or t 462tcgactcgct
cgtgcgcctc
tacggcacca acgccgacgc cttctcgtcg gacttcgccg 60cgtccatggt cangctcggc
aacatcggcc cgctcacggg ttccgcgggg gaggtcaggc 120tcaactgcag
gacggtgaat tcgaattcgt gacttcagga cggtgaatgc caccattttg
180aattggtact gctctttttc ccccacaagg agttgtagaa tttgcattcc
ttcaacgatt 240tcgaatttcg aatttgctgt ga 262463267DNAZea
maysmisc_feature(129)..(129)n is a, c, g, or t 463ccaccgacgg
cggcgagagt atatatacac acacacctga cgcctctgct gctgctggga 60tcaacgcggc
agcttagtga cgacgacgac tcttctgtct ctgcgttgat taatggacag
120taacagatnc atggcgacga gcggcgtggc tccgttcgtg gccaagacgt
accgcatggt 180ggatgacccg gcgaccgacg gcgtggtcgc gtggggcagg
gacaacaaca gcttcgtcgt 240ggccgacccc ttcgccttct cgcagac
267464201DNAZea maysmisc_feature(23)..(23)n is a, c, g, or
tmisc_feature(101)..(101)n is a, c, g, or
tmisc_feature(122)..(122)n is a, c, g, or
tmisc_feature(124)..(124)n is a, c, g, or
tmisc_feature(135)..(135)n is a, c, g, or
tmisc_feature(148)..(148)n is a, c, g, or
tmisc_feature(151)..(151)n is a, c, g, or
tmisc_feature(198)..(198)n is a, c, g, or t 464gaaaaggtta
aaccctattt ccntttcagt tagcataaaa aatctctatg atatgatatt 60caagagatga
tgaggcaaac tacgatgcta agtggcatga ncaagtcgtg ctctagtatc
120cnanaagata ggaangggtg taaaccanct ngaccatatt tgtaaaatgc
ttgagaagac 180ttccattatt ttccaacnag a 201465201DNAZea
maysmisc_feature(45)..(45)n is a, c, g, or tmisc_feature(68)..(68)n
is a, c, g, or tmisc_feature(79)..(79)n is a, c, g, or
tmisc_feature(101)..(101)n is a, c, g, or t 465actttagacc
ctaggccctt ggtcacccta taaatacccc cactngacag tggatggggc 60ccgcttcnca
agacatccnt gctcccctac caaccatttt ntcatgcatt gtttgtctca
120catgctcccg agttcttggg ccagtgtgta aggacccaac acacatatat
ggcaaatatc 180ttaataaaat caaatataag c 201466371DNAZea
maysmisc_feature(234)..(234)n is a, c, g, or t 466tatgaagcgg
actaggacat cgccggaggg gaagaagaac gggacatcgc cggaggggaa 60gaagaacgag
agcctcttct gatgcttgac ttcgtgtaca agttgtagct gaatgttcct
120caaaatatag gattaactag ttctgtaaca ctaacacccc ggcttggtta
tgacttagaa 180actgttaggc catgtttggt tcacatctcc taaagtttag
tcacttgttt aaanatgtta 240aaggaggtaa ctaaaaaaaa aacctcttaa
ataggacatt atagggacta atgtttagtt 300cctaaaattt aggagatgac
taaaggaacc aaacatagtt tactaagcct tagtaaacgt 360gtgaataaat g
371467201DNAZea maysmisc_feature(101)..(101)n is a, c, g, or
tmisc_feature(198)..(199)n is a, c, g, or t 467acatgctacc
gtgcagaatt cgagcaccaa gaacaaacta aattcagttc ccattatcac 60catcgctaac
accagagcag ttccttactc tacaaaccaa nttcattgac aattcagctt
120ctaactagaa agtcttctgt tctatttcta gaactaaccc gccatgaata
tcagttgtcc 180acaacataaa taagcaanng t 201468698DNAZea
maysmisc_feature(250)..(250)n is a, c, g, or
tmisc_feature(486)..(486)n is a, c, g, or
tmisc_feature(567)..(567)n is a, c, g, or
tmisc_feature(595)..(595)n is a, c, g, or
tmisc_feature(599)..(599)n is a, c, g, or
tmisc_feature(611)..(611)n is a, c, g, or
tmisc_feature(619)..(620)n is a, c, g, or
tmisc_feature(628)..(631)n is a, c, g, or
tmisc_feature(640)..(646)n is a, c, g, or
tmisc_feature(652)..(655)n is a, c, g, or
tmisc_feature(671)..(671)n is a, c, g, or
tmisc_feature(680)..(681)n is a, c, g, or
tmisc_feature(691)..(693)n is a, c, g, or
tmisc_feature(697)..(697)n is a, c, g, or t 468taagatagaa
agcaggagtc aattctgaat aacactagga gctaagtctg acaatgtata 60tattttgaag
gtaaaagatt tattaaatat gagtagcttc gtagctggga gagtgatcat
120tctcataatg aaacatggaa ctagtattgt atcatgataa gcaataagat
tagaataatt 180gaagaaagat catacaaatg ctccaggagc ccattctcga
tatgtgatac cttccgcgct 240acattggaan ataagataca aagttatttg
ctagtatata ggataaaaga taaacacttc 300atgttaaaga atacctgcga
ttaaatccaa acttctcata actacgggag aaggcttcca 360agcctccttc
atgttcatca atgtctgaac ggattcttct atagaggctg tacctgttct
420tttattcaag gaagttgaaa gcacagtgag atggatagtg gtgttcgcat
gggttatata 480taggantcct tataagaaga atacagaagg gatggaaatg
aaggttgcca aagaattaga 540acccgctgtt gcctctatat tgtctgncta
acatttctgt aaatatcctc tgaangagna 600catatcttgt ncgttcaann
gtaatgcnnn ntgaaaccan nnnnnntact tnnnncatca 660aagcagtatg
naagatttcn ngtgaaaacg nnnctgnc 698469708DNAZea
maysmisc_feature(191)..(191)n is a, c, g, or
tmisc_feature(238)..(238)n is a, c, g, or
tmisc_feature(309)..(310)n is a, c, g, or
tmisc_feature(326)..(326)n is a, c, g, or
tmisc_feature(366)..(366)n is a, c, g, or
tmisc_feature(404)..(404)n is a, c, g, or
tmisc_feature(411)..(411)n is a, c, g, or
tmisc_feature(432)..(432)n is a, c, g, or
tmisc_feature(450)..(450)n is a, c, g, or
tmisc_feature(484)..(484)n is a, c, g, or
tmisc_feature(565)..(566)n is a, c, g, or
tmisc_feature(578)..(580)n is a, c, g, or
tmisc_feature(601)..(601)n is a, c, g, or
tmisc_feature(604)..(604)n is a, c, g, or
tmisc_feature(618)..(618)n is a, c, g, or
tmisc_feature(650)..(652)n is a, c, g, or
tmisc_feature(659)..(662)n is a, c, g, or
tmisc_feature(664)..(664)n is a, c, g, or
tmisc_feature(666)..(666)n is a, c, g, or
tmisc_feature(668)..(682)n is a, c, g, or
tmisc_feature(685)..(696)n is a, c, g, or
tmisc_feature(698)..(699)n is a, c, g, or
tmisc_feature(703)..(704)n is a, c, g, or t 469tctgatgaca
gaagctgaaa gaaaactgaa ataaacagtg tctttacagg tggcaaatgt 60gatggatcat
ttctttaccc gtcattcagc ttctaacaca atcgctacag taaaagaacc
120ttatcggttt gtctcatcgc ttttgaaaca attaagctcc cgtccactca
aatggggtca 180ttcgttgtgc nctagcgcct gccattctcc accaccgcca
ccccacatca gattagantt 240gcatctaatc tgtggtttgt gtctttaatc
tcactttctg atggcgtagc agcctatcgt 300ctaaaggann ttcatatttg
aaacanagta aaaaatagaa tatgtagaac taaaaataaa 360taaagntaga
aaaatatagg attgaaaaac aaaggaaatt ttanaggagt nagtgttagt
420gtttggaaca cntgaatata agaatatgtn tattcctcta ttctagtgaa
taaaggcttg 480ctcntcattc tatcatgtat agaatatttt taaggaatga
gaacttgatt aggtgtacaa 540attttacttt ctctatatca cgaannaaaa
cgagcctnnn actggaaaaa taaaacttaa 600ntcnttcatt ctaaacanta
catgatgtaa aagtagaaat tggatttttn nnaaatgtnn 660nngnancnnn
nnnnnnnnnn nnctnnnnnn nnnnnngnnc tannactc 708470692DNAZea
maysmisc_feature(2)..(3)n is a, c, g, or tmisc_feature(180)..(180)n
is a, c, g, or tmisc_feature(426)..(426)n is a, c, g, or
tmisc_feature(557)..(558)n is a, c, g, or
tmisc_feature(590)..(590)n is a, c, g, or
tmisc_feature(633)..(634)n is a, c, g, or
tmisc_feature(638)..(640)n is a, c, g, or
tmisc_feature(648)..(649)n is a, c, g, or
tmisc_feature(651)..(651)n is a, c, g, or
tmisc_feature(659)..(660)n is a, c, g, or
tmisc_feature(667)..(667)n is a, c, g, or
tmisc_feature(669)..(672)n is a, c, g, or
tmisc_feature(679)..(684)n is a, c, g, or
tmisc_feature(686)..(691)n is a, c, g, or t 470cnncccccga
cgaccgcctt gccgatcgcc gccccgatcg cgccgacgtt cacggcgatc 60accgctattg
tggggatcag cagtggcgtc cactgcacca cgtacatctc agcgtacttc
120tcgttcacgg cgctcgtagc ctgcttcgcc gtcagcttga acgaaacgcc
gttcccgcgn 180aggactagct tcagcacgat gtgcagcgtc gctaacgggt
acacggctgt cgccccgatg 240atgtagaact gctcgttgcg gatccagtcc
agcagcgtga ggcctgccca cttgatctcc 300accatgccga tcagctctgt
cattgctatg atgacgacga ggtacagcac gtatgtcggg 360aacggcttct
ggatgtagaa ctcgccacgg aagatccata tgacggggaa gaggaggtag
420aacacnagga agaccgatga gattgggtag gctgtcatgt tggtgtaggc
gatccgttgc 480atcaagttca gtcgacggcc agcaaggagc gggcagtgtg
agaagaacat ttcaagggag 540ccgcccgacc agcgcannat ctggcagagg
cgctccgtga ggttgattgn tgcagtgcca 600cggaaggcat ccggctccat
gcggcagtac atnnatcnnn accctgtnng ntgtactcnn 660aagccgntnn
nnacgtccnn nnnngnnnnn nt 692471395DNAZea
maysmisc_feature(173)..(173)n is a, c, g, or t 471cgcggccgtg
gctgggaaac ggtggccggc gccatccttg aggtggtgtg acgatcgacg 60acgtgcatgg
cgtggcgcac agagctagta ataaaagggt agtgtacgct taccgcgtac
120gtacgtgtca ccgggcgtgg cactctccag tctccaggga cccatccacc
aantgctact 180gctccttcgt agggagacgt gggaataaag agtggtagct
gcatgcacgt acggcggcca 240tggctctccg atgagagagc tagctgtgta
cgtgtgttcg tgatgttgtt ccatgcatga 300catgtatacg tcttgcctaa
gtacgcttgt actagttgag agactgtgta agtgaaatgt 360gctataataa
taaataagta aagggcgcct tctcc 395472201DNAZea
maysmisc_feature(101)..(101)n is a, c, g, or t 472tatggctaga
ctataggtta attcaaagtg atagaatatt tatgaaaggg agaataaaag 60tgaaacccta
atgaatagtt tagtggttaa ctttgtgaaa ntaatcccat ttaatatatg
120atcccatctc tgaaatgact ttaggtaagt aagtaattca ttaaggtaga
tgtagttaag 180taatgtaatc tactgagata g 201473201DNAZea
maysmisc_feature(44)..(45)n is a, c, g, or
tmisc_feature(101)..(101)n is a, c, g, or
tmisc_feature(124)..(124)n is a, c, g, or t 473ataaggaatt
aaggagacac aaattagtct gaacgagtag gagnngtgga atttcattgg 60ataagaagga
tccaattagt ctgcaacgtg tgtggccacc naaaaagatt ctgcagttct
120ctanatctgc atatagtcca taggatcgga tatgctcctt ccatgttctt
tcgtttcaca 180aggtcaaagc tggtaccacg c 201474201DNAZea
maysmisc_feature(101)..(101)n is a, c, g, or t 474gtaatcgagg
ttcagatcaa gcatctatca tttactcggc aattgacaaa gcttataaag 60cttacggtcc
aaactcgttg aaggtatgtg aagaaccttg ngcacagtaa gggcacacgc
120atccatatga tgaaatagca tgaatggctg aactttgtgt gtgagatcaa
acttataata 180ttaagggcta gtttagaaac c 201475201DNAZea
maysmisc_feature(101)..(101)n is a, c, g, or t 475ctaggtctca
tgtggaggca tggagaacct gcttctcttc ctgacacatt ccaagcttta 60aaaaaactca
cacatgcccc gcatggcgct cgggatattc ngtacaagcg acgcgggtgc
120ctgcagcacc actaacaatc ctacaagaca acatactgac aataacaagg
cttccattaa 180tatagtatgg ggcttccata a 201476201DNAZea
maysmisc_feature(101)..(101)n is a, c, g, or
tmisc_feature(191)..(191)n is a, c, g, or t 476tccccaaacc
tgaaaccagc gaccaggcgg gggcaaaaaa aaaggctcac tgtaactgct 60tacaatgggg
tggtaattgc gtatcgagtg atcagatctc ntctcaccct ctccaggaac
120agtgggtggt caacgaacac gcggtccact ccgcgcttgt agcagtggaa
gaacctgacc 180gtctcgtacc ngtctcccat c 201477201DNAZea
maysmisc_feature(38)..(38)n is a, c, g, or tmisc_feature(64)..(64)n
is a, c, g, or tmisc_feature(71)..(71)n is a, c, g, or
tmisc_feature(101)..(101)n is a, c, g, or
tmisc_feature(152)..(152)n is a, c, g, or t 477ttattttagg
aaatgaggat tagggttagc gagtgagnat tttgcatttt tatatggggg 60cagngtgaga
ngatttgttg gaatcgaact tcataggccg ngcgagggga atgtatgact
120cctaatttgg gtcttcattg gatccccatg gnagaaattt catccccacc
ctgccatcct 180cgcaggggaa atttcatccc c 201478707DNAZea
maysmisc_feature(1)..(2)n is a, c, g, or tmisc_feature(37)..(37)n
is a, c, g, or tmisc_feature(412)..(412)n is a, c, g, or
tmisc_feature(586)..(587)n is a, c, g, or
tmisc_feature(619)..(623)n is a, c, g, or
tmisc_feature(633)..(633)n is a, c, g, or
tmisc_feature(635)..(635)n is a, c, g, or
tmisc_feature(648)..(654)n is a, c, g, or
tmisc_feature(663)..(664)n is a, c, g, or
tmisc_feature(666)..(669)n is a, c, g, or
tmisc_feature(673)..(673)n is a, c, g, or
tmisc_feature(679)..(680)n is a, c, g, or
tmisc_feature(682)..(685)n is a, c, g, or
tmisc_feature(690)..(691)n is a, c, g, or
tmisc_feature(697)..(699)n is a, c, g, or
tmisc_feature(701)..(704)n is a, c, g, or
tmisc_feature(706)..(706)n is a, c, g, or t 478nncaatgctt
gtcctgtttt ctttgcctca gtaagtngta tctttgtata ttttgctttg 60ccagatacag
gctgtaaaat gtaggcatgc tttttcgaag cagatctgga accatcctgc
120tcctgaaact caaaaacctg aaaatgtagt ttacaaacca ataaatattt
gttaggttgc 180tggatgcaag caacagaaga ataaaagatc ccaacaaaac
ctcattacag cactgcgtct 240attcacactc taccacatgt tttttcaaac
agaaattgac aaaggtaatt gtctccatgc 300caatttattc tctcaaaccc
agggtattta tttgcgcaca aacaaaattg ggaggcacct 360gactccactc
tgatggaagt aaatcctccc agggtttatc cacaatccaa gnactgctat
420cagagtcaaa atacaccgcc agactctcaa gttcaacaga ctgcaaaatg
ccaagttaaa 480gaagtcacgc aaattgatca aaagtaaaaa aggtcaaact
aaaacaaata agcacaccaa 540atgtgaaaaa tgcttgatgt gtgaagcgaa
aagaattcat tactanntcc tgaaaacttt 600aaatcaaatt aattgatcnn
nnnaattaga aantnaaaaa tgagctannn nnnnttttta 660ctnncnnnnc
tantattcnn cnnnntagan natgagnnnt nnnnant 707479764DNAZea
maysmisc_feature(108)..(129)n is a, c, g, or
tmisc_feature(444)..(444)n is a, c, g, or t 479tgggtacctc
gcctcgagcc ggaattcggc acgaggatcc aatcgaacca ccagtccacc 60acctgattga
ctagagcaaa agcacaagcc gcccacgcat ctcgattnnn nnnnnnnnnn
120nnnnnnnnng gcgcgcagag ctcgtgacga gagcaacctt ccttccgttc
ctcgatcgcc 180atggacaagg tgctggcctt ctcgatcctg agcgcgtcgc
cggccgacct ctcctccacg 240ggcgccggct tcggcgggag ctgggcgcgg
ctgtcgtggc ggcggggcgc ggacgaccag 300cgtgcgccgt ggtggtagca
ctataatcag caccaggagg aggacaggga gaagcgagac 360ttgcgctccc
gcgacggcgg agcgcacgcg agcggagggg gagcggcggc ggcgccaccg
420cggttcgcgc cggagtttga cggnatcgac tggttcggaa ccatcgtgtc
gcgctgatca 480acaatccggg ctcggccgac gcgccccccg agttaaccac
gtgaccaatc ctgtctacta 540tgtttttttt accttatggt ggattaattg
tcccaacaca gataattggg actccgcgtg 600ttgtacatac agggaactgc
tcaattacca ggtgggatgg ggaacattta tttgttcctg 660tcctctgcat
tttttttctg taccgaaatg gatggatggt ctccaacttg aaattgagtc
720cctcagcccc aggtaatctg gcggtggatg aacccaagcc gaac 764480201DNAZea
maysmisc_feature(101)..(101)n is a, c, g, or
tmisc_feature(201)..(201)n is a, c, g, or t 480atgagaagca
tgcatgcatg ggattgtccg attcctttct tcatgtagat atatacgatt 60gtatatgtat
gcatccattg ttcttgtctt ctttaataaa natgtaagtt atgcttgctt
120catgctttca acggagacga tgctggctca taagtacgtt gacagaaagg
cacaaaccca 180ctatatgtat atggcatggg n 201481201DNAZea
maysmisc_feature(1)..(1)n is a, c, g, or tmisc_feature(101)..(101)n
is a, c, g, or t 481ncgatcgatt taatgttgag ctggtgatat gggtgctgtt
tctttgatat ggcgtgtaga 60ttgggtcaaa actatgttaa taggcattga taaatggata
nggctattat tcttgccttt 120tcagttgcta gaattagggg attgaggttc
ggaatcaggc aattgcatag ttttgatgat 180tattcagcta gcaaatgaat t
201482201DNAZea maysmisc_feature(10)..(10)n is a, c, g, or
tmisc_feature(101)..(101)n is a, c, g, or
tmisc_feature(177)..(177)n is a, c, g, or t 482ctcctctggn
ccttcaagtg atgaaattgt tgggtgatct ggagagagat tcctttagga 60gaatattacc
ctgctttttc ccattattgg ctgatctgat ncgctgtgaa catagctctg
120gagaagttca acatgcactg tacaatatct tccaatcagc cattctcccc
atgatanggg 180tttaataaca gattgtgacc c 20148327DNAZea mays
483ccacaaaaaa tgaacaagaa gtgtcca 2748424DNAZea mays 484cccatggatg
atcggatgat gtta 2448524DNAZea mays 485tgttcttcgg ggtgttacta tcct
2448625DNAZea mays 486ctgtaaaaat gtctgctacg cttca 2548723DNAZea
mays 487ccctgcctag atacatgcaa agg 2348824DNAZea mays 488ttgctttgcc
ctcatgtaat cgta 2448923DNAZea mays 489tgacacatct gcaagcacaa att
2349028DNAZea mays 490gttcaacatt gacctttcca acaattct 2849121DNAZea
mays 491aactccacct tctggcaaag c 2149220DNAZea mays 492gctgttgcca
acccaaagaa 2049327DNAZea mays 493accgaaggag ttaccaagta tttgaag
2749423DNAZea mays 494gcatttaatt ttctgtccaa cgc 2349521DNAZea mays
495ggaacggatc ggatggattg g 2149625DNAZea mays 496cctcagccac
atgttagaat ttgta 2549725DNAZea mays 497cctgatgttc ctgaagtgca gatat
2549830DNAZea mays 498ctattacaat gactcaaccc atctttctct
3049919DNAZea mays 499cgccttctcg tcggacttc 1950025DNAZea mays
500gactcttctg tctctgcgtt gatta 2550124DNAZea mays 501tgaggcaaac
tacgatgcta agtg 2450224DNAZea mays 502gcccttggtc accctataaa tacc
2450325DNAZea mays 503gccatgtttg gttcacatct cctaa 2550424DNAZea
mays 504ctaacaccag agcagttcct tact 2450525DNAZea mays 505cacagtgaga
tggatagtgg tgttc 2550620DNAZea mays 506agctcccgtc cactcaaatg
2050720DNAZea mays 507cgccacggaa gatccatatg 2050818DNAZea mays
508cgtggcactc tccagtct 1850932DNAZea mays 509gggagaataa aagtgaaacc
ctaatgaata gt 3251024DNAZea mays 510aaggatccaa ttagtctgca acgt
2451123DNAZea mays 511cggtccaaac tcgttgaagg tat 2351217DNAZea mays
512tcacacatgc cccgcat 1751325DNAZea mays 513gtggtaattg cgtatcgagt
gatca 2551423DNAZea mays 514tgaggattag ggttagcgag tga 2351522DNAZea
mays 515aaatcctccc agggtttatc ca 2251616DNAZea mays 516ggttcgcgcc
ggagtt 1651727DNAZea mays 517tgtatgcatc
cattgttctt gtcttct 2751825DNAZea mays 518gcgtgtagat tgggtcaaaa
ctatg 2551923DNAZea mays 519cctgcttttt cccattattg gct 2352025DNAZea
mays 520atggcaagca catagtctaa tggat 2552127DNAZea mays
521ccatccaaat gtcaagtatg tccatct 2752224DNAZea mays 522aaccgaaccc
tgaataaaga aggt 2452320DNAZea mays 523ggttagctgc gaggcatcat
2052421DNAZea mays 524gctcagctag cagttggtga a 2152525DNAZea mays
525cagcaggaat ttatttcagc acact 2552622DNAZea mays 526caccaccaat
gaagcaagaa ca 2252726DNAZea mays 527tgggaaaatg agaatccttt gggaaa
2652820DNAZea mays 528gtcgtgatgg ccaatgattg 2052919DNAZea mays
529tatcgacctt cgccagcaa 1953025DNAZea mays 530agatcatgag catgggtgac
atttt 2553127DNAZea mays 531taacattggt ggtttggtgc tatataa
2753220DNAZea mays 532gctctgttac cgctgtttcg 2053321DNAZea mays
533gatgcctagg cggtgataag g 2153419DNAZea mays 534cgcaatgcta
gcgcaatgt 1953525DNAZea mays 535gtgtgtatag cctggaactg ttact
2553617DNAZea mays 536tgagcgggcc gatgttg 1753716DNAZea mays
537gccacgaacg gagcca 1653828DNAZea mays 538tggaaaataa tggaagtctt
ctcaagca 2853922DNAZea mays 539cgggagcatg tgagacaaac aa
2254031DNAZea mays 540aggaactaaa cattagtccc tataatgtcc t
3154130DNAZea mays 541gaacagaaga ctttctagtt agaagctgaa
3054225DNAZea mays 542cttcatttcc atcccttctg tattc 2554325DNAZea
mays 543gacacaaacc acagattaga tgcaa 2554422DNAZea mays
544catgacagcc tacccaatct ca 2254520DNAZea mays 545ccacgtctcc
ctacgaagga 2054627DNAZea mays 546cctaaagtca tttcagagat gggatca
2754725DNAZea mays 547ggagcatatc cgatcctatg gacta 2554822DNAZea
mays 548catatggatg cgtgtgccct ta 2254918DNAZea mays 549ttagtggtgc
tgcaggca 1855020DNAZea mays 550gttgaccacc cactgttcct 2055126DNAZea
mays 551ggatccaatg aagacccaaa ttagga 2655223DNAZea mays
552agagtctggc ggtgtatttt gac 2355317DNAZea mays 553cagcgcgaca
cgatggt 1755422DNAZea mays 554cgtctccgtt gaaagcatga ag
2255525DNAZea mays 555gaacctcaat cccctaattc tagca 2555625DNAZea
mays 556gcatgttgaa cttctccaga gctat 2555717DNAZea mays
557ttgagctttg gtttatc 1755815DNAZea mays 558ccactacaca aattc
1555914DNAZea mays 559tgcttcgtaa tttt 1456015DNAZea mays
560aggacactcc ctcac 1556114DNAZea mays 561ccgccgacgt tgtt
1456221DNAZea mays 562tgttggatgt tctaacgact g 2156315DNAZea mays
563aagccttcac agaaa 1556418DNAZea mays 564acacttaggg ctagtttg
1856515DNAZea mays 565cattggtgag gaagt 1556615DNAZea mays
566atttgccgcc attgt 1556715DNAZea mays 567aggaagccgg caaca
1556815DNAZea mays 568ttgcatgcat aataa 1556916DNAZea mays
569atctgatcca gaatct 1657015DNAZea mays 570ctatggcgta gcctt
1557114DNAZea mays 571cagcaaggtc ttca 1457219DNAZea mays
572tagttggtga cacttttgt 1957314DNAZea mays 573catggtcaag ctcg
1457414DNAZea mays 574tcgccatgga tctg 1457516DNAZea mays
575cacgacttga tcatgc 1657618DNAZea mays 576ccaaccattt tgtcatgc
1857719DNAZea mays 577cctcctttaa catttttaa 1957819DNAZea mays
578ctacaaacca aattcattg 1957921DNAZea mays 579ataaggattc ctatatataa
c 2158014DNAZea mays 580ttgtgcccta gcgc 1458115DNAZea mays
581aacacaagga agacc 1558216DNAZea mays 582atccaccaaa tgctac
1658318DNAZea mays 583aaatgggatt agtttcac 1858419DNAZea mays
584aatctttttg ggtggccac 1958519DNAZea mays 585tgaagaacct tgggcacag
1958620DNAZea mays 586ctcgggatat tcagtacaag 2058718DNAZea mays
587agagggtgag atgagatc 1858814DNAZea mays 588cctcgcacgg ccta
1458916DNAZea mays 589tccaagaact gctatc 1659013DNAZea mays
590tgacggcatc gac 1359122DNAZea mays 591caagcataac ttacatcttt at
2259222DNAZea mays 592caagaataat agccttatcc at 2259316DNAZea mays
593tcacagcgga tcagat 1659417DNAZea mays 594ttgagctttg atttatc
1759515DNAZea mays 595ccactacaaa aattc 1559616DNAZea mays
596ttgctttgta attttt 1659716DNAZea mays 597aggacattcc ctcacc
1659814DNAZea mays 598ccgccaacgt tgtt 1459919DNAZea mays
599ttggatgttc taacaactg 1960014DNAZea mays 600agcctccaca gaaa
1460117DNAZea mays 601cacttaggga tagtttg 1760215DNAZea mays
602cattggtgaa gaagt 1560314DNAZea mays 603ttgccgctat tgtt
1460415DNAZea mays 604aggaagccag caaca 1560514DNAZea mays
605ttgcacgcat aata 1460616DNAZea mays 606atctgatcaa gaatct
1660716DNAZea mays 607ctatggtgta gccttt 1660813DNAZea mays
608cagcaacgtc ttc 1360916DNAZea mays 609ttggtgacgc ttttgt
1661014DNAZea mays 610catggtcagg ctcg 1461113DNAZea mays
611cgccatgcat ctg 1361216DNAZea mays 612cacgacttgg tcatgc
1661318DNAZea mays 613ccaaccattt tttcatgc 1861419DNAZea mays
614cctcctttaa catgtttaa 1961517DNAZea mays 615acaaaccaag ttcattg
1761621DNAZea mays 616ataaggagtc ctatatataa c 2161715DNAZea mays
617tgtgctctag cgcct 1561817DNAZea mays 618aggtagaaca cgaggaa
1761916DNAZea mays 619atccaccaag tgctac 1662016DNAZea mays
620atgggattaa tttcac 1662117DNAZea mays 621tctttttcgg tggccac
1762219DNAZea mays 622tgaagaacct tgtgcacag 1962319DNAZea mays
623tcgggatatt cggtacaag 1962416DNAZea mays 624agggtgagac gagatc
1662513DNAZea mays 625ctcgcgcggc cta 1362616DNAZea mays
626caatccaagg actgct 1662714DNAZea mays 627tgacgggatc gact
1462821DNAZea mays 628aagcataact tacatgttta t 2162921DNAZea mays
629aagaataata gccctatcca t 2163016DNAZea mays 630tcacagcgaa tcagat
16
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