U.S. patent application number 17/480273 was filed with the patent office on 2022-01-13 for optimized method for producing a composition containing isomaltulose.
The applicant listed for this patent is Sudzucker Aktiengesellschaft Mannheim/Ochsenfurt. Invention is credited to Thomas Rose, Wolfgang Wach.
Application Number | 20220010345 17/480273 |
Document ID | / |
Family ID | |
Filed Date | 2022-01-13 |
United States Patent
Application |
20220010345 |
Kind Code |
A1 |
Wach; Wolfgang ; et
al. |
January 13, 2022 |
OPTIMIZED METHOD FOR PRODUCING A COMPOSITION CONTAINING
ISOMALTULOSE
Abstract
The present invention relates to a method for producing a
composition containing isomaltulose from a substrate containing
sucrose comprising the steps of: a) contacting the substrate
containing sucrose with a particulate carrier-immobilized sucrose
isomerase biomass and b) obtaining a composition containing
isomaltulose, characterized in that the median particle size d(0.5)
of the carrier-immobilized sucrose isomerase biomass is from 370 to
550 .mu.m. The carrier can be an alginate or a polyvinyl alcohol
carrier.
Inventors: |
Wach; Wolfgang; (Worms,
DE) ; Rose; Thomas; (Worms, DE) |
|
Applicant: |
Name |
City |
State |
Country |
Type |
Sudzucker Aktiengesellschaft Mannheim/Ochsenfurt |
Mannheim |
|
DE |
|
|
Appl. No.: |
17/480273 |
Filed: |
September 21, 2021 |
Related U.S. Patent Documents
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Application
Number |
Filing Date |
Patent Number |
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15879757 |
Jan 25, 2018 |
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17480273 |
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14905325 |
Jan 15, 2016 |
9920344 |
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PCT/EP2014/065358 |
Jul 17, 2014 |
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15879757 |
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International
Class: |
C12P 19/12 20060101
C12P019/12; C12P 19/24 20060101 C12P019/24; C12N 11/04 20060101
C12N011/04; C12N 11/02 20060101 C12N011/02 |
Foreign Application Data
Date |
Code |
Application Number |
Jul 18, 2013 |
DE |
10 2013 011 977.4 |
Claims
1. A method for producing a composition containing isomaltulose
from a substrate containing sucrose, comprising the steps of: a)
contacting the substrate containing sucrose with a particulate
carrier-immobilized sucrose isomerase biomass, wherein the carrier
is an alginate or polyvinyl alcohol carrier and b) obtaining a
composition containing isomaltulose, characterized in that the
median particle size d(0.5) of the carrier-immobilized sucrose
isomerase biomass is from 400 to 500 .mu.m, wherein the sucrose
isomerase biomass originates from Protaminobacter rubrum.
2. The method according to claim 1, wherein the particulate
carrier-immobilized sucrose isomerase biomass has d(0.5) from 420
to 470 .mu.m.
3. The method according to claim 1, wherein the particulate
carrier-immobilized sucrose isomerase biomass is spherical.
4. The method according to claim 1, wherein the weight ratio of
sucrose isomerase biomass to carrier is 10 to 6 parts sucrose
isomerase biomass to 6 to 2 parts of the carrier (in each case dry
weight).
5. The method according to claim 1, wherein the composition
containing isomaltulose contains trehalulose.
6. The method according to claim 1, wherein the sucrose isomerase
biomass is carrier-immobilized by bonding, crosslinking or
entrapment immobilization.
7. The method according to claim 1, wherein the sucrose isomerase
biomass is a sucrose isomerase, a microorganism cell with sucrose
isomerase activity or a cell-extract with sucrose isomerase
activity.
8. The method according to claim 1, wherein the method steps a) and
b) are carried out in a fixed bed.
9. The method according to claim 1, wherein the obtained
composition containing isomaltulose is catalytically
hydrogenated.
10. The method of claim 1 further comprising a catalytic
hydrogenation of the resultant composition containing isomaltulose
to obtain a composition containing sugar alcohol.
11. The method of claim 10, wherein the composition containing
sugar alcohol is isomalt or an isomalt variant.
12. The method of claim 1, wherein median particle size d(0.5) of
the carrier-immobilized sucrose isomerase biomass after drying is
from 400 to 500 .mu.m.
13. The method according to claim 1, wherein the method steps a)
and b) are carried out in a stirred tank.
Description
CROSS-REFERENCE TO RELATED APPLICATIONS
[0001] The present application is a continuation of and claims
priority to U.S. patent application Ser. No. 15/879,757, filed Jan.
25, 2018, which is a continuation of U.S. patent application Ser.
No. 14/905,325, filed Jan. 15, 2016 (now U.S. Pat. No. 9,920,344),
which claims priority to International Application No.
PCT/EP2014/065358 filed Jul. 17, 2014, which claims priority to
German Application No. 10 2013 011 977.4 filed Jul. 18, 2013, the
contents of which are all incorporated herein by reference.
FIELD OF THE INVENTION
[0002] The present invention relates to a method for producing a
composition containing isomaltulose from a substrate containing
sucrose.
BACKGROUND OF THE INVENTION
[0003] It is known that isomaltulose (palatinose,
6-O-.alpha.-D-glucopyranosyl-D-fructose) can be produced by means
of a biotechnological process through enzymatic isomerization from
sucrose. Isomaltulose is a physiologically valuable sugar, with
increasing significance as a sucrose substitute in foodstuffs and
related products. Isomaltulose is acariogenic and has a low
glycemic index while having essentially the same energy content as
sucrose. Isomaltulose has been approved for use in foodstuffs since
2005. Isomaltulose serves also as raw material for the production
of the sugar alcohol isomalt, a racemic mixture of 1,6-GPS
(6-O-.alpha.-D-glucopyranosyl-D-sorbitol) and 1,1-GPM
(1-O-.alpha.-D-glucopyranosyl-D-mannitol) as well as modifications
thereof, in particular 1,6-GPS- or 1,1-GPM-enriched mixtures.
[0004] In the known biotechnological systems, sucrose is not
completely isomerized enzymatically to isomaltulose. Rather,
further isomerization products and byproducts are generated.
Trehalulose is an important further isomerization product.
[0005] Trehalulose (1-O-.alpha.-D-glucopyranosyl-D-fructose) as a
further important isomer of sucrose can be found in nature, for
example in honey and, like isomaltulose, is not cariogenic.
[0006] Both isomers, isomaltulose and trehalulose, are known for
example from EP 1 424 074 A1 as hetero-disaccharides which,
compared to sucrose in the small intestine of human and animal
consumers, have a reduced hydrolysis rate and present themselves as
advantageous food components, in particular such that can keep
blood sugar levels under better control.
[0007] Isomaltulose and trehalulose are produced on a large scale
by using enzymatic rearrangement of immobilized bacterial cells or
fragments thereof. In this respect, the
.alpha.1-.alpha.2-glycosidic bond existing between the
monosaccharide units of the sucrose disaccharide is isomerized into
an .alpha.1-.alpha.6-bond with isomaltulose or an
.alpha.1-.alpha.1-bond with trehalulose, respectively. This
rearrangement of sucrose into the two acariogenic disaccharides
takes place by catalysis of the bacterial enzyme sucrose isomerase
(E.C. 5.4.99.11, synonym: sucrose mutase, saccharose mutase,
sucrose isomerase, isomaltulose synthase). Microorganisms that have
sucrose isomerase activity and which can be used in
biotechnological processes, are in particular Protaminobacter
rubrum, Erwinia rhapontici, Pseudomonas mesoacidophila, Pantoea
dispersa and Serratia plymuthica. Depending on the organism used
and the reaction conditions, the result of this reaction is a
product mix, which can contain not only the desired acariogenic
disaccharides isomaltulose and trehalulose in different proportions
but also possibly unwanted monosaccharides, for example glucose
and/or fructose, as well as oligomers.
[0008] EP 0 483 755 B1 discloses methods for the production of
compositions containing trehalulose and isomaltulose, for which
purpose Pseudomonas mesoacidophila MX-45 (FERM-BP 3619) or
Agrobacterium radiobacter MX-232 (FERM-BP 3620) is used to obtain a
product containing predominantly trehalulose.
[0009] The use of enzymatic activities from Pseudomonas putida,
Thermus ruber, Thermus aquatica or Pimelobacter for producing
compositions containing predominantly trehalulose from solutions
containing sucrose is known from EP 0 794 259 B1.
[0010] EP 0 091 063 A2 and EP 0 625 578 A1 disclose methods for the
production of isomaltulose using immobilized bacterial cells, in
particular Protaminobacter rubrum (CBS 574.77). The disclosed
methods are based on a solution containing sucrose, which is
transformed into a product containing predominantly isomaltulose
but also trehalulose, using immobilized cells.
[0011] The disclosed methods are all characterized by a limited
activity of sucrose isomerase, and where in particular for
large-scale production of isomaltulose, trehalulose or both, an
improved process efficiency is desirable, in particular a faster
turnover rate and/or increased conversion rate and/or yield.
[0012] Therefore, the present invention aims to solve the technical
problem to overcome the aforementioned disadvantages and in
particular to provide a method, in the frame of which a conversion
of sucrose to a product containing isomaltulose and/or trehalulose
is achieved that is improved as compared to the prior art. In
particular, it is an aim of the present invention to provide an
increased turnover rate and/or an increased rate of conversion
and/or yield.
[0013] The present invention solves the technical problem it aims
to solve by providing the teaching according to the independent
claim.
[0014] Specifically, the present invention provides the following
teachings.
[0015] The present invention relates in particular to an inventive
method for the production of a composition containing isomaltulose
from a substrate containing sucrose comprising the following steps:
[0016] a) contacting the substrate containing sucrose with a
particulate carrier-immobilized sucrose isomerase biomass and
[0017] b) obtaining a composition containing isomaltulose,
characterized in that the particulate carrier-immobilized sucrose
isomerase biomass has a particle diameter dp of 100 to 1300 .mu.m,
in particular 100 to 900 .mu.m.
[0018] The present invention relates in particular to an inventive
method wherein the particulate carrier-immobilized sucrose
isomerase biomass is spherical.
[0019] In a particularly preferred embodiment, the particulate
carrier-immobilized sucrose isomerase biomass can have the shape of
a cube, a plate, a particle, a fiber, particularly a hollow fiber,
a sphere, a hollow sphere or a LentiKat.RTM..
[0020] The present invention relates in particular to an inventive
method wherein the weight ratio of sucrose isomerase biomass to
carrier is 10 to 6, preferably 7, parts sucrose isomerase biomass
to 6 to 2, preferably 3, parts carrier (each as dry mass).
[0021] In a particularly preferred embodiment, the weight ratio of
sucrose isomerase biomass to carrier is 0.2 to 0.8, in particular
0.3 to 0.7, or preferably 0.4 to 0.6.
[0022] The present invention relates in particular to an inventive
method wherein the composition containing isomaltulose contains
trehalulose.
[0023] The present invention relates in particular to an inventive
method wherein the sucrose isomerase biomass is carrier-immobilized
by adsorption, bonding, in particular covalent bonding,
crosslinking, encapsulation or entrapment immobilization.
[0024] The present invention relates in particular to an inventive
method wherein the carrier is an alginate or polyvinyl alcohol
carrier, in particular a sodium alginate carrier.
[0025] The present invention relates in particular to an inventive
method wherein the sucrose isomerase biomass is a sucrose
isomerase, a microorganism cell with sucrose isomerase activity or
a cell-extract with sucrose isomerase activity.
[0026] The present invention relates in particular to an inventive
method wherein the sucrose isomerase biomass originates from
microorganisms of the genera Escherichia, Salmonella, Serratia,
Erwinia, Enterobacter, Klebsiella, Raoultella, Pectobacterium,
Pseudomonas, Azotobacter, Pantoea, Leucanea, Protaminobacter or
Bacillus sp.
[0027] The present invention relates in particular to an inventive
method wherein the sucrose isomerase biomass originates from
Protaminobacter rubrum, Klebsiella sp., particularly strain LX3 or
strain NK33-98-8, Klebsiella pneumoniae, especially strain 342;
Enterobacter sp., particularly strain SZ62 or strain FMB1, Erwinia
tasmaniensis, particularly strain Et1/99; Pectobacterium
atrosepticum, particularly strain SCRI 1043; Pectobacterium
carovotum, particularly subspecies brasiliensis, particularly
strain PBR 1692, Azotobacter vinelandii, Leucanea leucocephalia,
Erwinia rhapontici, Raoultella planticola, Pseudomonas
mesoacidophila, Leuconostoc mesenteroides, Pantoea dispersa,
Serratia plymuthica, Serratia marcescens or Agrobacterium
radiobacter.
[0028] The present invention relates in particular to an inventive
method wherein the process steps a) and b) are carried out in a
fixed bed or stirred tank.
[0029] The present invention particularly relates to an inventive
method, wherein the obtained composition containing isomaltulose is
hydrogenated catalytically.
[0030] The inventive method is preferably carried out continuously,
but it can also be carried out semi-continuously or batch-wise.
[0031] The present invention relates in particular to an inventive
method for producing a composition containing sugar alcohol,
wherein an inventive method for producing a composition containing
isomaltulose made from a substrate containing sucrose and then a
catalytic hydrogenation of the resultant composition containing
isomaltulose are carried out and a composition containing sugar
alcohol is obtained.
[0032] The present invention also relates to a method for producing
a composition containing sugar alcohol according to the present
invention, wherein the composition containing sugar alcohol is
isomalt or an isomalt variant.
[0033] In connection with the present invention, a "substrate
containing sucrose" means a composition containing 1 to 100% by
weight, preferably 1 to 99% by weight, preferably 10 to 95% by
weight, in particular 20 to 90% by weight of sucrose (in each case
referring to the weight of the dry substance of the substrate).
[0034] In one embodiment, the substrate containing sucrose can
consist solely of sucrose.
[0035] In another embodiment, the substrate containing sucrose can,
in addition to sucrose, contain one, two, three, several or many
more substances, in particular selected from the group consisting
of trehalulose, isomelezitose, glucose, fructose, sucrose,
isomaltose, trisaccharides and oligomers.
[0036] In a particularly preferred embodiment, the substrate
containing sucrose preferably has a sucrose content preferably of
2% by weight to preferably 85% by weight, preferably of 3% by
weight up to preferably 90% by weight, preferably from 4% by weight
up to preferably 95% by weight, preferably of 5% by weight up to
preferably 96% by weight, preferably from 6% up to preferably 97%
by weight, preferably from 7% by weight up to preferably 98% by
weight, preferably from 8% up to preferably 99% by weight,
preferably from 9% up to preferably 100% by weight, preferably from
10% by weight up to preferably 85% by weight, preferably from 20%
by weight up to preferably 90% by weight, preferably from 30% by
weight up to preferably 95% by weight, preferably from 40% by
weight up to preferably 96% by weight, preferably from 50% by
weight up to preferably 97% by weight, preferably from 60% by
weight up to preferably 98% by weight, preferably from 70% by
weight up to preferably 99% by weight or preferably from 80% by
weight up to preferably 100% by weight (in each case referring to
the weight of the dry substance of the substrate).
[0037] In a particularly preferred embodiment, the substrate
containing sucrose includes a content of sucrose of preferably 5%
by weight up to preferably 75% by weight, preferably of 10% by
weight up to preferably 78% by weight, preferably of 20% up to
preferably 85% by weight, preferably of 30% by weight up to
preferably 88% by weight, preferably of 40% by weight up to
preferably 93% by weight, preferably of 50% by weight up to
preferably 94% by weight, preferably of 60% by weight up to
preferably 95% by weight, preferably of 65% by weight up to
preferably 96% by weight, preferably of 10% by weight up to
preferably 97% by weight, preferably of 20% by weight up to
preferably 98% by weight, preferably of 30% by weight up to
preferably 99% by weight, preferably of 40% up to preferably 100%
by weight (in each case referring to the dry matter weight of the
substrate).
[0038] Especially preferred is a sucrose content of preferably 60
to 90% by weight, preferably 70 to 80% by weight, preferably 30 to
60% by weight, preferably 40 to 50% by weight (in each case
referring to the weight of the dry substance of the substrate
containing sucrose).
[0039] In a particularly preferred embodiment, the substrate
containing sucrose comprises 90 to 99% by weight, preferably 90 to
98% by weight, in particular 90 to 97% by weight of sucrose (based
in each case on the dry matter weight of the substrate).
[0040] In a particularly preferred embodiment, the substrate
containing sucrose is present in liquid form, preferably in
dissolved or suspended form, preferably in a liquid, particularly
aqueous, medium. In a particularly preferred embodiment, the
aqueous medium is water. In a particularly preferred embodiment,
the substrate containing sucrose is present in an aqueous medium,
namely in an aqueous solution or aqueous suspension.
[0041] In a particularly preferred embodiment, the aqueous medium
containing the substrate that contains sucrose, i.e. for example
the solution or suspension, comprises 0.1 to 80% by weight,
preferably 1 to 70% by weight, preferably 4 to 60% by weight,
preferably 5 to 50% by weight, especially 5 to 40% by weight,
especially from 5 to 30% by weight, preferably from 35 to 45% by
weight, preferably from 20 to 27%, preferably 40 to 75% by weight,
especially 40 to 60% by weight, especially 10 to 60% by weight,
preferably 20 to 55% by weight of the substrate containing sucrose,
wherein these quantity ranges add up with the weight of the medium,
i.e. for example water, to 100% (% by weight, each referring to the
total weight of the medium containing the sucrose containing
substrate, corresponding to the dry matter content of the substrate
that contains sucrose in the medium).
[0042] In a particularly preferred embodiment, the solution or
suspension that contains the sucrose-containing substrate can be
thin juice or thick juice from a sugar processing plant, preferably
with a dry matter content of 5 to 70%, preferably from 50 to 70%,
in particular 55 to 68%, particularly from 5 to 30%, preferably 20
to 27% (% by weight, in each case referring to the total weight of
the medium containing the sucrose substrate).
[0043] According to the invention it is also possible to use
molasses or other impure compositions containing sucrose as a
sucrose-containing substrate medium, in particular a solution or
suspension.
[0044] In a further preferred embodiment, the liquid medium
containing the sucrose-containing substrate, in particular the
solution or suspension that contains the sucrose-containing
substrate, has a sucrose content of 0.1 to 80% by weight, 5 to 30%
by weight, 20 to 30% by weight, 20 to 60% by weight, 30 to 60% by
weight, 35 to 45% by weight, 40 to 75% by weight, 40 to 60% by
weight, 10 to 60% by weight or preferably 20 to 55% by weight (in
each case referring to the total weight of the liquid medium
containing the sucrose).
[0045] In connection with the present invention, a "composition
containing isomaltulose" is understood to be the product of
isomerization of the activity of a sucrose isomerase on a substrate
containing sucrose. A composition containing isomaltulose of the
present invention contains a mixture of isomaltulose and
trehalulose, in particular consists thereof. Preferably, in one
embodiment, more isomaltulose than trehalulose is present, and in
another embodiment more trehalulose is present than isomaltulose.
Further components of a composition containing isomaltulose can be
isomelezitose, fructose, glucose, isomaltose, saccharose,
trisaccharides, oligomers, or mixtures of two or more of these
sugars.
[0046] Particularly preferably, the proportion of isomaltulose in
the composition containing isomaltulose is at least 80% by weight,
preferably at least 81% by weight, at least 82% by weight, at least
83% by weight, at least 84% by weight, at least 85% by weight,
preferably at least 86% by weight and in particular at least 87% by
weight, at least 88% by weight, at least 89% by weight, at least
90% by weight, at least 91% by weight, at least 92% by weight or
93% by weight (in each case referring to the dry matter of the
composition containing isomaltulose). The rest of the components
adding up to a 100% of such a composition containing isomaltulose
are formed by trehalulose and optionally isomelezitose, fructose,
glucose, sucrose, isomaltose, trisaccharides, oligomers or two or
more thereof.
[0047] In a particularly preferred embodiment, the composition
containing isomaltulose comprises 60 to 98% by weight, in
particular 70 to 95% by weight, preferably 75 to 88% by weight, in
particular 75 to 84% by weight isomaltulose (in each case referring
to the dry matter of the composition containing isomaltulose).
[0048] In a further preferred embodiment, the composition
containing isomaltulose can comprise from 1 to 20% by weight, 5 to
20% by weight, 10 to 30% by weight or 20 up to 99% by weight,
preferably 20 to 40% by weight, preferably 20 to 30% by weight,
preferably from 30 up to preferably 95% by weight, preferably from
45 to 96% by weight, preferably from 46 to 97% by weight,
preferably from 47 to 98% by weight, preferably from 48 to 99% by
weight, preferably from 49 to 99% by weight, preferably from 40 up
to 98% by weight, preferably from 50 to 97% by weight, from 60 to
96% by weight or preferably from 70 up to 97% by weight, preferably
from 80 up to 98% by weight, preferably from 75 up to 98% by
weight, particularly 50 to 99% by weight, particularly 60 to 98% by
weight, particularly from 85 up to 99% by weight and preferably 95%
by weight to 99% by weight, preferably from 96 to 99% by weight,
preferably from 97 to 99% by weight, preferably from 98 to 99% by
weight of trehalulose (in each case referring to the dry matter of
the composition containing isomaltulose). The rest of the
components adding up to a 100% of the total composition is formed
by isomaltulose and optionally glucose, fructose, isomelezitose,
isomaltose, sucrose, trisaccharides, oligomers or two or more of
them.
[0049] In a particularly preferred embodiment, the composition
containing isomaltulose is 60 to 90% by weight of isomaltulose, 5
to 40% by weight of trehalulose and 0 to 5% by weight of at least
one further substance selected from the group consisting of
isomelezitose, sucrose, fructose, glucose, isomaltose,
trisaccharides and oligomers.
[0050] In a further preferred embodiment, the present composition
containing isomaltulose is 60 to 90% by weight of trehalulose, 5 to
40% by weight of isomaltulose and 0 to 5% by weight of at least one
further substance selected from the group consisting of
isomelezitose, fructose, glucose, sucrose, isomaltose,
trisaccharides and oligomers.
[0051] In a further preferred embodiment, the present composition
containing isomaltulose is in liquid form, preferably in dissolved
or suspended form, preferably in a liquid, particularly aqueous,
medium. In a particularly preferred embodiment, the aqueous medium
is water. In a particularly preferred embodiment, the composition
containing isomaltulose is in an aqueous medium, namely in an
aqueous solution or aqueous suspension.
[0052] In a particularly preferred embodiment, the aqueous medium
containing the composition that contains the isomaltulose, i.e. for
example the solution or suspension, comprises 0.1 to 80% by weight,
preferably 1 to 70% by weight, preferably 4 to 60% by weight,
preferably 5 to 50% by weight, especially 5 to 40% by weight,
especially from 5 to 30% by weight, preferably from 35 to 45% by
weight, preferably from 20 to 27%, preferably 40 to 75% by weight,
especially 40 to 60% by weight, especially 10 to 60% by weight,
preferably 20 to 55% by weight of the substrate containing
isomaltulose, wherein these quantity ranges add up with the weight
of the medium, i.e. for example water, to 100% (% by weight, each
referring to the total weight of the medium containing the
composition that contains isomaltulose, corresponding to the dry
matter content of the composition containing isomaltulose in the
medium).
[0053] In connection with the present invention, the "sucrose
isomerase biomass" is a biomass, in particular a cell, a cell
extract, an enzyme or an enzyme mixture, wherein the biomass is
capable under appropriate conditions of converting a substrate
containing sucrose into a composition containing isomaltulose, i.e.
exhibiting a sucrose isomerase activity. Preferably, the sucrose
isomerase has the activity of a glucosyl transferase or of a
sucrose-6-glucosyl mutase (EC 5.4.99.11).
[0054] In an advantageous way, the cells that can be used as
sucrose isomerase biomass can be alive or dead. In a particularly
preferred embodiment, the cells used as a sucrose isomerase biomass
can be complete or destroyed, in particular ground cells, cell
fragments, cell lysates or cell extracts. The inventively used
sucrose isomerase biomass can in a preferred embodiment be
purified, in particular be isolated, for example by conventional
purification methods such as solvent extraction, French Press,
lyophilization and/or enzymatic treatment. The sucrose isomerase
biomass used can be of natural origin or it can be genetically
modified in one embodiment.
[0055] The inventively provided step a) of contacting the substrate
containing sucrose with a carrier-immobilized sucrose isomerase
biomass is performed preferably according to the invention in such
a way that a conversion of the substrate containing sucrose into a
composition containing isomaltulose can take place, preferably in a
way to optimize turnover.
[0056] Preferably, contacting is carried out at a temperature of 10
to 40.degree. C., preferably 15 to 40.degree. C., preferably 10 to
37.degree. C., preferably 25 to 40.degree. C., preferably 25 to
30.degree. C., preferably 30 to 40.degree. C., preferably 10 to
25.degree. C., preferably 15 to 30.degree. C., preferably 18 to
26.degree. C., preferably 10 to 20.degree. C., in particular 10 to
17.degree. C.
[0057] In a further preferred embodiment, it is provided that the
method step a) is carried out at a pH value from 5.0 to 9.0,
preferably 5.0 to 7.0, preferably 6.0 to 7.0.
[0058] In connection with the present invention,
carrier-immobilized sucrose isomerase biomass is understood to be a
sucrose isomerase biomass immobilized at or in a carrier. This will
be referred to below as immobilisate. Immobilization can have
occurred in a conventional manner, for example by adsorption,
bonding, in particular covalent bonding, crosslinking,
encapsulation or entrapment immobilization.
[0059] In a particularly preferred manner, the present invention
aims to produce the particulate carrier-immobilized sucrose
isomerase biomass by supplying biomass in a first method step x1),
for example by extraction in a fermenter, and by mixing this
biomass in a second method step x2) with an alginate solution and
introducing, especially by dripping, the biomass-alginate mixture
in a third method step x3) into a calcium salt solution, especially
a calcium chloride or calcium acetate solution. The particles that
are forming will gel and in a fourth method step x4) are dried, for
example in a fluidized bed dryer.
[0060] In a particularly preferred embodiment, it can also be
provided to concentrate, i.e. thicken, the supplied biomass
provided in the first method step x1) before mixing it with the
alginate solution.
[0061] In a particularly preferred embodiment, in step x3) it is
provided to introduce the biomass-alginate mixture into a calcium
chloride solution by dripping under pressure, in particular by
means of compressed air, i.e. in a blow-off method. In a further
preferred embodiment, in step x3), the biomass-alginate mixture can
be dripped into the calcium salt solution by means of an
electrostatic process, in particular under the application of a
voltage to the calcium-salt solution and the biomass-alginate
mixture. In a further embodiment, the process step x3) can be
carried out by vibrating the biomass-alginate mixture, for example
by means of a punch, thus allowing a targeted dropping of this
mixture into the calcium salt solution. Where appropriate, an
increased pressure, for example, an increased air pressure, can be
used.
[0062] In a further embodiment, the biomass-alginate mixture can be
dripped into the calcium salt solution in method step x3) using a
lateral blow-off pressure. In a further preferred embodiment, in
step x3), the biomass-alginate mixture can be placed on a rotating
disk and in this way can drip into the calcium salt solution. In a
further preferred embodiment, the biomass-alginate mixture can be
dripped into the calcium salt solution in method step x3) by means
of rotating nozzles.
[0063] In a further preferred embodiment, the biomass-alginate
mixture can be dripped into the calcium salt solution in method
step x3) by means of a Jet-Cutter.RTM.. In a further preferred
embodiment, the biomass-alginate mixture in method step x3) can be
dripped into the calcium salt solution by means of multi-nozzle
systems in combination with belt dryers and air drying, optionally
with the use of cutting tools.
[0064] The aforementioned method steps, in particular the
immobilization techniques described above according to process step
x3), provide in an advantageous way the inventively preferred
particle diameter dp.
[0065] In connection with the present invention, a particulate
carrier-immobilized sucrose isomerase biomass is understood to be a
carrier-immobilized sucrose isomerase biomass which is present in
particle form, i.e. that are present in the form of particles, in
particular in the form of solid particles.
[0066] In a preferred embodiment, the particulate
carrier-immobilized sucrose isomerase biomass has, in dry form, a
particle diameter dp of 100-1300 .mu.m, preferably 200-1300 .mu.m,
preferably 150-1200 .mu.m, preferably 150 to 1000 .mu.m, preferably
100 to 900 .mu.m, preferably 150 to 950 .mu.m, preferably 150 to
900 .mu.m, preferably 150 to 850 .mu.m, in particular 100 to 800
.mu.m, preferably 200 to 800 .mu.m, in particular 200 to 950 .mu.m,
in particular 250 to 500 .mu.m, in particular 300 to 700 .mu.m,
preferably 400 to 600 .mu.m, in particular 450 to 550 .mu.m, in
particular 480 to 540 .mu.m, preferably 500 .mu.m (in each case
referring to the dry support-immobilized sucrose isomerase
biomass).
[0067] The present invention relates in particular to an inventive
method wherein the particulate carrier-immobilized sucrose
isomerase biomass has a particle diameter dp of 400 to 600 .mu.m,
in particular 100 to 400 .mu.m, in particular 100 to 300 .mu.m, in
particular 200 to 300 .mu.m, preferably 250 to 300 .mu.m.
[0068] In a particularly preferred embodiment, the particulate
carrier-immobilized sucrose isomerase biomass has a particle
diameter dp of 250 to 500 .mu.m.
[0069] In a particularly preferred embodiment, the particulate
support-immobilized sucrose isomerase biomass in dry form has a
volumetric mean value D[4,3] in the range from 380 to 570 .mu.m, in
particular a volumetric mean value D[4,3] in the range from 380 to
400 .mu.m, 450 to 490 .mu.m or 530 to 580 .mu.m.
[0070] In a further preferred embodiment, the particulate
support-immobilized sucrose isomerase biomass in dry form has a
D[3,2] value in a range from 360 to 540 .mu.m, especially 360 to
380, 430 to 450 or 510 to 530 .mu.m.
[0071] In a further preferred embodiment, the particulate
support-immobilized sucrose isomerase biomass in dry form has a
specific surface area in a range from 0.0100 to 0.0180, in
particular in a range from 0.0100 to 0.0118 or 0.0130 to 0.0140 or
0.0150 to 0.0170 m.sup.2/g.
[0072] In a further particularly preferred embodiment, the
particulate support-immobilized sucrose isomerase biomass in dry
form has a uniformity in a range from 0.190 to 0.210.
[0073] In a further preferred embodiment, the particulate
support-immobilized sucrose isomerase biomass has a d(0.1) value in
a range from 270 to 400 .mu.m, in particular in a range from 270 to
290 .mu.m, 320 to 340 .mu.m or 380 to 400 .mu.m.
[0074] In a further preferred embodiment, the particulate
support-immobilized sucrose isomerase biomass in dry form has a
d(0.5) value in a range from 370 to 550 .mu.m, in particular 370 to
390 .mu.m, 450 to 470 .mu.m or 530 to 550 .mu.m.
[0075] In a further preferred embodiment, the particulate
support-immobilized sucrose isomerase biomass in dry form has a d
(0.9) value in a range from 510 to 760 .mu.m, particularly 510 to
530 .mu.m, 610 to 640 .mu.m or 730 to 760 .mu.m.
[0076] Unless otherwise stated in the present teaching, all
parameters specified for the characterization of the particulate
support-immobilized sucrose isomerase biomass, such as the specific
surface area, the particle diameter, the volumetric mean values or
the d (0.1), d (0.5), d (0.9) values, refer to particles measured
in dry form.
[0077] In a particularly preferred embodiment, the particulate
support-immobilized sucrose isomerase biomass in dry form has a
particle diameter dp of 100 to 500, especially 100 to 450,
especially 100 to 400, especially 100 to 300, especially 200 to
300, preferably 250 to 300 .mu.m.
[0078] In connection with the present invention, the term "d(0.5)
value" or the term "particle diameter d(0.5)" denotes the median
value of the particle size distribution based on the mass or volume
distribution, wherein d(0, 5) indicates that 50% of particulate
matter is less than the respective value specified.
[0079] Correspondingly, the term d(0.1) respectively d(0.9) means
that 10 resp. 90% of the particulate matter is less than the
respective value specified.
[0080] The particle size distribution is a volumetric particle size
distribution.
[0081] In a particularly preferred embodiment, the determination of
the particle size distribution of the produced particulate
support-immobilized sucrose isomerase biomass is carried out by
using laser diffraction, in particular according to the procedure
of example 1 of the present teaching.
[0082] In a particularly preferred embodiment, the particulate
carrier-immobilized sucrose isomerase biomass is spherical.
[0083] The carrier-immobilized sucrose isomerase biomass in a
preferred embodiment has a uniformity coefficient <1, 2.
[0084] The uniformity coefficient is calculated using the following
formula.
Uniformity .times. .times. coefficient = X i .times. d .function. (
x , 0 . 5 ) - d i d .function. ( x , 0 . 5 ) .times. X i
##EQU00001##
[0085] In a particularly preferred embodiment, the
carrier-immobilized sucrose isomerase biomass has a specific
activity of at least 400 units/g dry matter, preferably at least
450 units/g dry matter, preferably at least 500 units/g dry matter,
especially at least 600 units/g dry matter, especially at least 700
units/g dry matter, preferably at least 800 units/g dry matter.
[0086] In connection with the present invention, a method according
to the present invention is understood to mean in particular a
method according to the above steps a) and b) for obtaining a
composition containing isomaltulose, wherein the particulate
carrier-immobilized sucrose isomerase biomass has a particle
diameter dp of the above characterized inventive kind, in
particular from 100 to 1300 .mu.m, in particular from 150 to 1300
.mu.m, preferably from 100 to 900 .mu.m (in each case measured in
dry form). A method of the present invention is also an especially
advantageous embodiment of such a method, wherein the various
specific embodiments of the inventive method can also be combined
with one another, inasmuch as these combinations do not technically
exclude one another.
[0087] Further advantageous embodiments of the invention result
from the dependent claims.
[0088] The invention is further illustrated by way of the following
examples and the corresponding figures.
DESCRIPTION OF THE DRAWINGS
[0089] The figures show:
[0090] FIG. 1 graphically illustrates the size distribution of a
first inventive particle preparation (coarse grain),
[0091] FIG. 2 shows the size distribution of a second inventive
particle preparation (normal grain), and
[0092] FIG. 3 shows the size distribution of a third inventive
particle preparation (fine grain).
DETAILED DESCRIPTION OF THE INVENTION
Example 1
[0093] Determination of particle size distribution of immobilized
biocatalysts by means of laser diffraction using a particle size
analyzer (MASTERSIZER 2000, Malvern Instruments Ltd.) dry
measurement.
Execution
[0094] With the help of laser diffraction measurement, the particle
size distribution of the biocatalyst (immobilisate) according to
the invention is determined. To describe the size distribution, the
parameters d.sub.0,1, d.sub.0,5, d.sub.0,9 and the uniformity are
shown.
Short Description
[0095] The measurement principle is based on the scattered
light/laser diffraction spectroscopy ISO 13320. Scattered particles
are brought in low concentration into a laser beam. The
introduction of the particles is effected by sucking the dry
biocatalyst sample into the measuring cell ("dry measurement").
Depending on the diameter of the particles, there is a diffraction
of the laser light that is collected by detectors as the scattered
radiation. The result of the measurement is initially in the form
of light intensities measured at the detectors and must be
converted into a particle size distribution. This is achieved using
the customary evaluation software for particles >1 .mu.m given
here by an approximation of Joseph von Fraunhofer (1814).
Equipment/Means
[0096] Test sieve with a diameter of 200 mm in accordance with DIN
ISO 3310-1 of mesh size 1.25 mm [0097] Collecting tray and cover
for the test sieve [0098] Malvern MASTERSIZER 2000 with dry
dispersion unit Scirocco 2000 (A), including associated control and
analysis software, Malvern Instruments Ltd.
Sample Preparation
[0098] [0099] All weighing operations are performed at .+-.0.01 g
(reading accuracy). [0100] Determine tare weights (tare) of the
sieve and of the collecting tray [0101] Assemble collecting tray
and sieve [0102] Determine weight of the complete biocatalyst
sample (usually in a 250-mL vial) of each of the biocatalyst batch
[0103] Transfer sample quantitatively onto the sieve, cover it and
sieve by hand [0104] After sieving, weigh both the sieve with the
residue and the collecting base with the passed volume (gross)
[0105] Evaluation/calculation: the percentage of coarse fraction of
the biocatalyst sample is determined from the sieve residue [0106]
The sieve residue is discarded and the passed volume in the
collecting tray is returned to the original sample bottle
Measurement with the Malvern MASTERSIZER 2000
Dosing/Feeding of the Sample
[0107] The dosage of the samples is carried out via a vibrating
channel with adjustable gap width. A coarse sieve with several
balls is used as sieve strainer.
[0108] The dosage (gap width) needs to be adjusted depending on the
product so that the measured concentration (green area) is
achieved.
Dispersing Medium
[0109] Air with a certain pressure is used as a dispersing
medium.
[0110] The measurements are performed with the MASTERSIZER 2000,
dispersing unit Scirocco 2000 (A).
Example 2
A. Preparation of the Biocatalyst
[0111] Cells from an inoculation of the strain Protaminobacter
rubrum (CBS 574.77) are washed off with 10 ml sterile nutrient
substrate consisting of 8 kg thick juice from a sugar plant (dry
substance content=65%), 2 kg of corn steeping liquor, 0.1 kg
(NH.sub.4).sub.2 HPO.sub.4 and 89.9 kg distilled water, adjusted to
pH 7.2 if necessary. This suspension is used as inoculum for the
shaking machine pre-culture in 1 liter flasks containing 200 ml of
nutrient solution of the above composition.
[0112] After a 30-hour incubation at 29.degree. C., 18 liters of
nutrient solution of the above composition are inoculated with 10
flasks each (total content 2 liter) in a 30-liter small fermenter
and fermented at 29.degree. C. with 20 liters of air per minute and
a stirrer speed of 350 rpm.
[0113] After reaching germinal counts of more than 5.times.10.sup.9
germs/ml, the fermentation is stopped; the cells are harvested from
the fermenter solution by centrifugation and suspended in a 2%
sodium alginate solution.
[0114] By using a rotation immobilization, a jet cutting process or
pulsating drip method, in particular using electrostatic methods,
blow-off or vibration methods, in particular by using a lateral
blow-off pressure, rotating disks or rotating nozzles, the
suspension is dripped in a 2% calcium chloride solution and the
inventively preferred particle diameters and particle size
distributions, in particular with a particle diameter dp of 250-500
.mu.m (based on dry particles) are provided. Multi-nozzle systems
using a belt dryer are also used.
[0115] Table 1 shows the characterizing parameters achieved for
three exemplary obtained biocatalyst preparations, namely for a
first preparation, referred to as coarse grain, a second
preparation, referred to as normal grain, and a third preparation,
referred to as fine grain.
TABLE-US-00001 TABLE 1 Vol. mean d(0, 1) d(0, 5) d(0, 9) value D[4,
3] D[3, 2] Spec. surface (.mu.m) (.mu.m) (.mu.m) (.mu.m) (.mu.m)
(m.sup.2/g) Uniformity Coarse grain 392.690 539.575 749.682 559.012
524.745 0.0114 0.207 Normal grain 335.826 457.546 626.839 470.798
444.496 0.0135 0.198 Fine grain 280.522 381.883 523.390 393.435
371.278 0.0162 0.202
[0116] The resulting immobilization balls are washed with water.
This biocatalyst can be stored at +4.degree. C. for several
weeks.
B. Production of the Composition Containing Isomaltulose
[0117] The immobilized cells obtained as described in A are filled
into a temperature-controlled column reactor, heated up to 25 to
30.degree. C. and continuously passed through by a sucrose solution
with 35 to 45% dry matter content. The flow rate is set such that
at least 97% of the sucrose used is rearranged.
TABLE-US-00002 TABLE 2 Turnover rate Weight dm Used Feed stream
sucrose/w. dm rel. activity biocatalyst [mL/h] immobilisates/h %
Mean value 120 2.3 95 coarse grain Mean value 126 2.4 100 normal
grain Mean value 149 2.8 118 fine grain Production 4500 l/h 2.25 94
(800 kg dm)
[0118] Table 2 shows the catalytic activity of immobilisates of
different particle diameters according to Table 1 in a 100 ml fixed
bed.
[0119] A HPLC analysis of the composition containing isomaltulose
emerging from the column reactor resulted in the following
composition:
TABLE-US-00003 TABLE 3 Iso- Iso- residual Fructose Glucose Sucrose
maltulose Trehalulose Isomaltose DP-3 melizitose saccharides 2.7
2.0 0.9 85.1 8.0 0.8 0.3 0.3 <0.1 2.7 2.0 0.9 85.2 8.0 0.7 0.2
0.2 <0.1 2.7 2.0 0.9 85.3 7.8 0.8 0.2 0.3 <0.1
[0120] Product range according to Table 2 (coarse grain, normal
grain, fine grain) (indications in g/100 g dry matter).
D. Hydrogenation of the Composition Containing Isomaltulose
[0121] The respective preparations, freed from the residual
sucrose, of the composition containing isomaltulose were
continuously hydrogenated on Raney nickel at 80.degree. C. with
hydrogen gas under pressure of about 10 MPa. After nickel
separation and purification by ion exchange, the preparations of
the composition containing isomaltulose hydrogenated under neutral
conditions had about the following composition:
TABLE-US-00004 TABLE 4 Mannitol 1.5% of dry matter Sorbitol 4.0% of
dm 1,6-GPS 44.4% of dm 1,1-GPS 3.8% of dm 1,1-GPM 45.3% of dm
hydrogenated and non- 1.0% of dm hydrogenated oligomers
Example 3
[0122] To prepare this biocatalyst, cells from an inoculation of
the strain Pseudomonas mesoacidophila MX-45 (FERM 1 1808) were
washed off with 10 ml sterile nutrient substrate consisting of 8 kg
thick juice from a sugar plant (dry substance content=65%), 2 kg of
corn steeping liquor, 0.1 kg (NH.sub.4).sub.2 HPO.sub.4 and 89.9 kg
distilled water adjusted to pH 7.2. This suspension is used as
inoculum for the shaking machine pre-culture in a 1-liter flask
containing 200 ml of nutrient solution.
[0123] After a 30-hour incubation at 29.degree. C., 18 liters of
nutrient solution of the above composition were inoculated with 10
flasks each (total content 2 liter) in a 30-liter small fermenter
and fermented at 29.degree. C. with 20 liters of air per minute and
a stirrer speed of 350 rpm.
[0124] After reaching germinal counts of more than 5.times.10.sup.9
germs/ml, the fermentation was stopped; the cells were harvested
from the fermenter solution by centrifugation and suspended in a 2%
sodium alginate solution.
[0125] By using rotation immobilization, a jet cutting process or
pulsating drip method, in particular using electrostatic methods,
blow-off or vibration methods, in particular by using a lateral
blow-off pressure, rotating disks or rotating nozzle, the
suspension is dripped into a 2% calcium chloride solution and the
inventively preferred particle diameters and particle size
distributions, in particular with a particle diameter dp according
to table 1 (based on dry particles) are provided. Multi-nozzle
systems using a belt dryer are also used.
[0126] The resulting immobilization balls were washed with water.
This biocatalyst can be stored at +4.degree. C. for several
weeks.
[0127] In order to produce a composition containing isomaltulose,
the immobilized cells of Pseudomonas mesoacidophila MX-45 (FERM
11808) obtained in such a way were filled into a
temperature-controlled column reactor, heated up to 25 to
30.degree. C. and continuously passed through by a sucrose solution
with 35 to 45% dry matter content. The flow rate is set such that
at least 97% of the sucrose used was rearranged.
[0128] A HPLC analysis of the composition containing isomaltulose
emerging from the column reactor resulted in the following
composition:
TABLE-US-00005 TABLE 5 Fructose 0.2% of dm Glucose 0.2% of dm
Sucrose 1.0% of dm Isomaltulose 12.5% of dm Isomaltose 0.2% of dm
Trehalulose 85.7% of dm Oligomers (DP > 3) 0.2% of dm
[0129] The composition containing isomaltulose produced in this way
was freed from the residual sucrose and continuously hydrogenated
on Raney nickel at about 80.degree. C. with hydrogen gas under
pressure of 8 to 12 MPa.
[0130] After nickel separation and purification by ion exchange,
the composition containing isomaltulose hydrogenated under neutral
conditions had the following composition:
TABLE-US-00006 TABLE 6 Mannitol 0.4% of dm Sorbitol 1.0% of dm
1.1-GPM 57.7% of dm 1.1-GPS 34.4% of dm 1.6-GPS 6.4% of dm
hydrogenated and non- 0.2% of dm hydrogenated oligomers
[0131] In order to remove the hydrogenated and non-hydrogenated
oligomers as well as sorbitol from the product by chromatographic
separation, the chromatographic separation, after the hydrogenation
with a chromatographic separation column, was performed with a
strongly acidic cation exchange resin loaded with sodium or
potassium ions.
* * * * *