U.S. patent application number 17/144846 was filed with the patent office on 2022-01-06 for continuous isolation of cannabidiol and conversion of cannabidiol to delta 8-tetrahydrocannabinol and delta 9-tetrahydrocannabinol.
The applicant listed for this patent is SOCATI TECHNOLOGIES - OREGON, LLC. Invention is credited to Joon CHO, Mark G. TEGEN.
Application Number | 20220002259 17/144846 |
Document ID | / |
Family ID | 1000005843734 |
Filed Date | 2022-01-06 |
United States Patent
Application |
20220002259 |
Kind Code |
A1 |
TEGEN; Mark G. ; et
al. |
January 6, 2022 |
CONTINUOUS ISOLATION OF CANNABIDIOL AND CONVERSION OF CANNABIDIOL
TO DELTA 8-TETRAHYDROCANNABINOL AND DELTA
9-TETRAHYDROCANNABINOL
Abstract
In alternative embodiments, provided are processes comprising
the continuous isolation and purification of cannabinoids and
further isomerization of the purified cannabidiol to
.DELTA..sup.8tetrahydrocannabinol (.DELTA..sup.8THC) and
.DELTA..sup.9tetrahydrocannabinol (.DELTA..sup.9THC). In
alternative embodiments, provided are processes for converting
.DELTA.8-THC into .DELTA..sup.9-THC. In alternative embodiments,
provided are processes for the industrial scale continuous
isolation and purification of cannabinoids and further
isomerization of the purified cannabidiol to .DELTA..sup.9-THC.
Inventors: |
TEGEN; Mark G.; (Seattle,
WA) ; CHO; Joon; (Tumwater, OR) |
|
Applicant: |
Name |
City |
State |
Country |
Type |
SOCATI TECHNOLOGIES - OREGON, LLC |
Austin |
TX |
US |
|
|
Family ID: |
1000005843734 |
Appl. No.: |
17/144846 |
Filed: |
January 8, 2021 |
Related U.S. Patent Documents
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Application
Number |
Filing Date |
Patent Number |
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16672047 |
Nov 1, 2019 |
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17144846 |
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16388182 |
Apr 18, 2019 |
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16672047 |
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PCT/US2019/021138 |
Mar 7, 2019 |
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16388182 |
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62715545 |
Aug 7, 2018 |
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62697923 |
Jul 13, 2018 |
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62697920 |
Jul 13, 2018 |
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62697926 |
Jul 13, 2018 |
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62639608 |
Mar 7, 2018 |
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Current U.S.
Class: |
1/1 |
Current CPC
Class: |
C07D 301/32 20130101;
C07D 311/86 20130101 |
International
Class: |
C07D 301/32 20060101
C07D301/32; C07D 311/86 20060101 C07D311/86 |
Claims
1. A method or process for obtaining or purifying a substantially
pure .DELTA..sup.9 THC from a natural or a synthetic source,
comprising: (a) obtaining or being provided a first extract,
aliquot or sample of the natural or synthetic source comprising a
cannabidiol (CBD) and/or .DELTA..sup.9 THC and/or .DELTA..sup.8 THC
and/or other cannabinoids from the natural or the synthetic source;
(b) dissolving the first extract aliquot or sample in a first
solvent, or diluting the first extract aliquot or sample in a first
solvent; (c) loading the solvent-dissolved or diluted first extract
aliquot or sample onto a first chromatography column, wherein the
.DELTA..sup.9 THC and/or .DELTA..sup.8 THC and/or other
cannabinoids are reversibly bound to the first chromatography
column; (d) eluting the .DELTA..sup.9 THC and/or .DELTA..sup.8 THC
and/or other cannabinoids off the first chromatography column with
an elution solvent mixture, wherein optionally the eluting is a
gradient elution; (e) collecting or isolating the .DELTA..sup.9 THC
and/or .DELTA..sup.8 THC and/or other cannabinoids from said
elution solvent mixture; (f) adding a second solvent of opposite
polarity (to the elution solvent mixture) to said elution solvent
mixture; (g) removing all or substantially most of the second
solvent, thereby leaving a first extract comprising a plurality of
cannabinoids comprising the .DELTA..sup.9 THC and/or .DELTA..sup.8
THC and/or other cannabinoids; (h) dissolving the first extract
comprising the plurality of cannabinoids in a first reaction
solvent; (i) adding a catalyst to the first extract comprising the
plurality of cannabinoids and the first reaction solvent, wherein
the catalyst can be added before, simultaneous with or after
addition of the first reaction solvent to the extract comprising
the plurality of cannabinoids; and (j) reacting the first extract
comprising the plurality of cannabinoids, the catalyst and the
first reaction solvent, wherein optionally the reaction converts
.DELTA..sup.8 THC to .DELTA..sup.9 THC; (k) adding a neutralizing
agent; (l) removal of the catalyst and the neutralizing agent to
generate a first reaction product; (m) optionally adding a second
reaction solvent to the first reaction product; (n) optionally
removing all or substantially most of the second reaction solvent,
thereby leaving a second extract comprising a plurality of
cannabinoids; (o) adding a stabilization agent to the second
extract; (p) adding an elimination agent to the second extract; (q)
removal or removal of substantially all of the stabilization agent
and the elimination agent, thereby leaving a third extract; (r) and
if the second reaction solvent is not removed in step (n), adding a
third solvent of opposite polarity to the third extract; (s)
removing the second reaction solvent, if present and if not removed
in step (n), and removing all or substantially most of the third
solvent of opposite polarity, if added, thereby leaving a fourth
extract; (t) dissolving third or the fourth extract in a second
solvent, wherein optionally the second solvent is the same or
substantially the same as the first solvent; (u) loading the
dissolved third or the fourth extract onto a second chromatography
column, wherein optionally the chromatography column is a normal
phase chromatography column or a reverse phase chromatography
column, wherein the plurality of cannabinoids are reversible bound
to the second chromatography column, and optionally the second
chromatography column is the same as the first chromatography
column; and (v) eluting the dissolved third or the fourth extract
from the second column with an elution solution, wherein optionally
the elution is by a gradient elution process and the .DELTA..sup.9
THC and/or .DELTA..sup.8 THC and/or other cannabinoids are
separately collected in different or separate gradient elution
fractions; and (w) optionally collecting the plurality of
cannabinoids from the elution solution, and/or removing all or
substantially most of the elution solvent, thereby obtaining or
purifying a substantially pure plurality of cannabinoids, wherein
optionally the plurality of cannabinoids comprises .DELTA..sup.9
THC and/or .DELTA..sup.8 THC.
2. The method or process of claim 1, wherein the CBD content of the
first extract, aliquot or sample material is greater than about
25%, 50%, 75%, 90% or 95% by weight, or is between about 5% and 95%
by weight, by weight of the material.
3. The method or process of claim 1, wherein the first solvent
and/or the second solvent comprises methanol, propanol, ethanol,
isobutanol, butanol, isopropanol, tetrahydrofuran, chloroform,
acetone, hexane, methylene dichloride, and/or dichloroethane; and
optionally the first solvent and/or the second solvent comprises
chloroform, methylene dichloride and methanol, propanol and/or
ethanol.
4. The method or process of claim 1, wherein the chromatography
column comprises a silica gel, an alumina or an ion exchange
material, and optionally the ion exchange column comprises an anion
exchange material; and optionally the chromatography column is a
normal phase chromatography column, ion exchange column or reverse
phase column.
5. The method or process of claim 1, wherein the elution solution
comprises a solvent, and the solvent comprises a one or a mixture
of: ethylene dichloride; chloroform; or, methylene dichloride; and
an alcohol comprising methanol, ethanol, propanol or butanol or a
mixture thereof; an acid comprising acetic acid, formic acid,
oxalic acid, glycolic acid or a mixture thereof; and/or, ammonium
hydroxide, or the elution solvent is or comprises a
dichloromethane, dichloroethane, or chloroform and methanol or
ethanol.
6. The method or process of claim 1, wherein the first reaction
solvent comprises ethylene dichloride, chloroform, methylene
dichloride, toluene, xylene, benzene, hexane and/or pentane, or
mixtures thereof.
7. The method or process of claim 1, wherein the catalyst is or
comprises a Lewis acid or a non-oxidizing acid catalyst, and
optionally the catalyst comprises p-toluenesulfonic acid.
8. The method or process of claim 1, wherein: (a) the neutralizing
agent is or comprises an alkali, or the neutralizing agent is or
comprises sodium bicarbonate, sodium carbonate, calcium hydroxide
or ammonium hydroxide; (b) the stabilization agent is or comprises
zinc chloride or methylene chloride; (c) the second reaction
solvent comprises dimethyl sulfoxide, water, dimethylformamide,
methanol, saline, chloroform, propanol, ethanol, isobutanol,
formamide, butanol, isopropanol, tetrahydrofuran, dioxane, benzene,
toluene and/or xylene or a mixture thereof, and optionally the
second reaction solvent comprises chloroform, hexane,
dichloromethane, dichloroethane, benzene or toluene or a mixture
thereof; (d) the elimination agent is or comprises a strong base,
and optionally the strong base comprises: lithium hydroxide (LiOH),
sodium hydroxide (NaOH), potassium hydroxide (KOH), rubidium
hydroxide (RbOH), cesium hydroxide (CsOH), magnesium hydroxide
(Mg(OH)2), calcium hydroxide (Ca(OH)2), strontium hydroxide
Sr(OH)2), barium hydroxide Ba(OH)2), potassium hydride and/or
sodium hydride, potassium tert-pentoxide, organic superbases,
bispidines, multicyclic polyamines, organometallic compounds of
reactive metals, wherein optionally the reactive metals comprise
organolithium, organo-magnesium, lithium diisopropylamide, n-butyl
lithium and potassium tert-butoxide, sodium methoxide, or sodium
ethoxide; and optionally the elimination agent is or comprises
potassium tert- pentoxide, lithium diisopropylamide and/or
potassium tert-butoxide; (e) the elution solution is or comprises a
solvent or mixture of solvents; or (f) the extract is filtered
before being loaded on to the column, and optionally the extract is
mixed with silica, diatomaceous earth (DE), bentonite clay (BC), or
equivalents, or mixtures thereof, before filtering, and optionally
the extract is chilled before being mixed with the silica, DE, BC
or equivalents or is chilled before being filtered.
9. The method or process of claim 1, wherein: (a) the microbial
material comprises or is derived from a bacterium, an algal cell, a
lichen or a yeast, or a recombinant bacterium, lichen, algal cell
or yeast cell; (b) the plant or starting material comprises or is
derived from a botanical drug substance, including hemp or a
cannabis plant, wherein optionally the cannabis plant is a Cannabis
sativa, a Cannabis chemovar, or variants thereof, or a
decarboxylated Cannabis plant material; and optionally the plant or
starting material comprises oils or extracts from a trichome or a
trichome fraction of a pubescent plant, or an algae or a lichen;
and optionally the oils or extracts are from the plant or microbial
material by: washing, contacting or exposing the trichome or
trichome fraction, or the pubescent plant, algae or lichen, with:
at least one non-polar, organic solvent; at least one polar,
organic solvent; or, a mix of at least one non-polar, organic
solvent with (and) at least one polar, organic solvent.
10. The method or process of claim 1, wherein: (a) the microbial
material comprises or is derived from a bacterium, an algal cell, a
lichen or a yeast, or a recombinant bacterium, lichen, algal cell
or yeast cell; (b) the plant or starting material comprises or is
derived from a botanical drug substance, including hemp or a
cannabis plant, wherein optionally the cannabis plant is a Cannabis
sativa, a Cannabis chemovar, or variants thereof, or herbal
Cannabis or dried Cannabis biomass, or a decarboxylated Cannabis
plant material; and optionally the plant material comprises or is
derived from any plant part, wherein any plant part comprises bark,
wood, leaves, stems, roots, flowers, fruits, seeds, berries or
parts thereof, and exudate; and optionally the plant or starting
material comprises oils or extracts from a trichome or a trichome
fraction of a pubescent plant, or an algae or a lichen; and
optionally the oils or extracts are from the plant or microbial
material by: washing, contacting or exposing the trichome or
trichome fraction, or the pubescent plant, algae or lichen, with:
at least one non-polar, organic solvent; at least one polar,
organic solvent; or, a mix of at least one non-polar, organic
solvent with (and) at least one polar, organic solvent.
11. The method of claim 1, wherein the natural source comprises a
plant or a microbial material, or a material derived from a plant
source.
12. The method of claim 1, wherein the chromatography column is a
normal phase chromatography column, an ion exchange chromatography
column, or a reverse phase chromatography column.
13. The method of claim 1, wherein the substantially most of the
extract aliquot or sample material not reversibly bound to the
first column at the first station is removed with a first wash
solution before the column is moved to a next or a second
station.
14. The method of claim 1, wherein the elution comprises use of a
gradient elution process and the .DELTA..sup.9 THC and/or
.DELTA..sup.8 THC and/or other cannabinoids are separately
collected in different or separate gradient elution fractions.
15. The method of claim 14, wherein if the elution comprises use of
a gradient elution process: in a first gradient solvent non-polar
compounds and terpenes are eluted off the column; in a second
gradient solvent cannabidiol (CBD) is eluted off the column; in a
third gradient a CBD/tetrahydrocannabinol (THC) mix is first eluted
and then remaining THC is eluted off the column; in a fourth
gradient remaining cannabinoids are eluted off the column, wherein
optionally the remaining cannabinoids comprise CBC, CBG, CBN; and,
in a fifth gradient solvent all remaining polar compounds are
eluted off the column, wherein the remaining polar compounds
comprises cannabidiolic acid (CBDA) and tetrahydrocannabinolic acid
(THCA).
16. The method of claim 1, wherein the elution comprises use of a
gradient elution process comprising use of a reverse phase column
chromatography, and the gradient elution: begins with an 80:20 or a
70:30 methanol to water elution solution or equivalent, which
elutes off the column some or substantially most of the polar
compounds, wherein optionally the polar compounds comprise CBDA
and/or TGCA; then the gradient elution moves to an 85:15 or an
80:20 methanol to water elution solvent or equivalent, which elutes
off the column some or substantially most of the CBD; then the
gradient elution moves to a 90:10 methanol to water elution
solution or equivalent, which initially elutes off the column a mix
of the remaining CBD and THC, and finally elutes off the column
THC; and finally the gradient elution moves to a 100% methanol
elution solution, which removes a mix of remaining cannabinoids
comprising CBC, CBG, CBN.
17. The method of claim 1, wherein the elution comprises use of a
gradient elution process and the .DELTA..sup.9 THC and/or
.DELTA..sup.8 THC and/or other cannabinoids are separately
collected in different or separate gradient elution fractions.
18. The method of claim 1, wherein if the elution is by a gradient
elution process: in a first gradient solvent non-polar compounds
and terpenes are eluted off the column; in a second gradient
solvent cannabidiol (CBD) is eluted off the column; in a third
gradient a CBD/tetrahydrocannabinol (THC) mix is first eluted and
then remaining THC is eluted off the column; in a fourth gradient
remaining cannabinoids are eluted off the column, wherein
optionally the remaining cannabinoids comprise CBC, CBG, CBN; and,
in a fifth gradient solvent all remaining polar compounds are
eluted off the column, wherein the remaining polar compounds
comprises cannabidiolic acid (CBDA) and tetrahydrocannabinolic acid
(THCA), and optionally, if the elution is by a gradient elution
process using reverse phase column chromatography, the gradient
elution: begins with an 80:20 or a 70:30 methanol to water elution
solution or equivalent, which elutes off the column some or
substantially most of the polar compounds, wherein optionally the
polar compounds comprise CBDA and/or TGCA; then the gradient
elution moves to an 85:15 or an 80:20 methanol to water elution
solvent or equivalent, which elutes off the column some or
substantially most of the CBD; then the gradient elution moves to a
90:10 methanol to water elution solution or equivalent, which
initially elutes off the column a mix of the remaining CBD and THC,
and finally elutes off the column THC; and finally the gradient
elution moves to a 100% methanol elution solution, which removes a
mix of remaining cannabinoids comprising CBC, CBG, CBN.
19. The method of claim 1, wherein in step (g) the other
cannabinoids comprise CBC, CBG, CBN.
20. The method of claim 1, wherein the second reaction solvent
comprises dimethyl sulfoxide, water, dimethylformamide, methanol,
ethylene dichloride, chloroform, propanol, ethanol, isobutanol,
formamide, methylene dichloride, butanol, isopropanol,
tetrahydrofuran, dioxane, benzene, toluene, xylene, or combinations
thereof.
Description
FIELD OF THE INVENTION
[0001] This invention generally relates to the industrial scale
processing and purification of cannabinoids. In alternative
embodiments, provided are processes comprising the continuous
isolation and purification of cannabinoids and further
isomerization of the purified cannabidiol to
.DELTA..sup.8-tetrahydrocannabinol (.DELTA..sup.8-THC) and
.DELTA..sup.9-tetrahydrocannabinol (.DELTA..sup.9-THC). In
alternative embodiments, provided are processes for converting
.DELTA..sup.8-THC into .DELTA..sup.9-THC. In alternative
embodiments, provided are processes for the industrial scale
continuous isolation and purification of cannabinoids and further
isomerization of the purified cannabidiol to .DELTA..sup.9-THC.
BACKGROUND
[0002] Cannabinoids are a class of diverse chemical compounds that
acts on cannabinoid receptors in cells that alter neurotransmitter
release in the brain. They are of great value in pharmaceutical
applications. Ligands for these receptor proteins include the
endocannabinoids (produced naturally in the body by animals) and
the phytocannabinoids (found in hemp, Cannabis and some other
plants). Among these cannanbinoids is cannabidiol (CBD) widely
considered to have a range of potential medical applications
including treatment of Dravet syndrome, a rare form of epilepsy
that is difficult to treat that begins in infancy. Another
cannabinoid is .DELTA..sup.9tetrahydrocannabinol (.DELTA..sup.9THC)
that acts as an appetite stimulant for people with AIDS and
antiemetic for people receiving chemotherapy.
[0003] Tetrahydrocannabinol (THC) is the principal psychoactive
constituent of cannabis. Natural phytocannabinoids sources such as
hemp and Cannabis contain at least 113 different cannabinoids.
Currently mixtures comprising terpenes, lipids, waxes, chlorophyll
and other plant matter, including cannabinoids, are extracted in
mass using polar solvent extraction, nonpolar solvent extraction or
mixtures thereof and then physically separated using multiple steps
including distillation, chromatography, winterization or
combinations of these processes.
[0004] Recently, public interest in Cannabis as medicine has been
growing, based in no small part on the fact that Cannabis has long
been considered to have medicinal properties, ranging from
treatment of cramps, migraines, convulsions, appetite stimulation
and attenuation of nausea and vomiting. In fact, a report issued by
the National Academy of Sciences' Institute of Medicine indicated
that the active components of Cannabis appear to be useful in
treating pain, nausea, AIDS-related weight loss or "wasting",
muscle spasms in multiple sclerosis as well as other problems.
Advocates of medical marijuana argue that Cannabis is also useful
for glaucoma, Parkinson's disease, Huntington's disease, migraines,
epilepsy and Alzheimer's disease.
[0005] Marijuana refers to varieties of Cannabis having a high
content of .DELTA..sup.9-tetrahydrocannabinol (.DELTA..sup.9-THC),
which is the psychoactive ingredient of marijuana, whereas
industrial hemp refers to varieties of the Cannabis plant that have
a low content of .DELTA..sup.9-THC.
[0006] Furthermore, .DELTA..sup.9-THC is only one of a family of
about 60 bi- and tri-cyclic compounds named cannabinoids. For
example, .DELTA..sup.8-THC is a double bond isomer of
.DELTA..sup.9-THC and is a minor constituent of most varieties of
Cannabis (see, e.g., Hollister and Gillespie, 1972, Clin Pharmacol
Ther 14: 353). The major chemical difference between the two
compounds is that .DELTA..sup.9-THC can be oxidized to cannabinol
whereas .DELTA..sup.8-THC does not and is in fact very stable.
.DELTA..sup.8-THC, for the most part, produces similar psychometric
effects as does .DELTA..sup.9-THC, but is generally considered to
be 50% less potent than .DELTA..sup.9-THC and has been shown in
some cases to be 3-10 times less potent. .DELTA..sup.8-THC has also
been shown to be more (200%) effective an anti-emetic than
.DELTA..sup.9-THC and has been used as an anti-emetic in children,
based on the belief that the side effects of .DELTA..sup.9-THC and
.DELTA..sup.8-THC, such as anxiety and dysphoria, are more
prevalent in adults than children (Abrahamov et al, 1995, Life
Sciences 56: 2097-2102). On the other hand, CBD has no activity on
its own when administered to humans. It is of note that CBD is
typically about 2% (0.54%) dry weight of hemp chaff,
.DELTA..sup.8-THC is approximately 0.2% (0.05-0.5%) dry weight and
.DELTA..sup.9-THC is approximately 0.1% (0.05-0.3%).
[0007] The large variety of cannabinoids and other plant matter
present in extracts makes it difficult to separate the cannabinoids
from each other as well as from the non-cannabinoid plant matter
and achieve a high enough purity level to meet pharmaceutical or
analytical requirements. Separations can be achieved using various
techniques including such as distillation which subjects the
cannabinoid rich mixture of heat history which can degrade the
cannabinoids. For separations that do not subject the cannabinoids
to heat history chromatography is applied however the current state
of the art is to apply these techniques in a non-continuous method
which severely limits the scale and efficiency of the separation
methodology.
[0008] There remains a need for a method that is simple, efficient
and continuous process to first generate purified cannabidiol and
combined with a simple, efficient method to convert cannabidiol
into a mixture of .DELTA..sup.8THC and .DELTA..sup.9-THC. Clearly,
as the cannabinoids are of potential medicinal value, improved
methods of both isolating CBD and converting CBD to
.DELTA..sup.8-THC and .DELTA..sup.9-THC are needed.
SUMMARY
Chapt. 1
[0009] In alternative embodiments, provided are processes and
methods for purifying cannabinoids and converting cannabidiol to
.DELTA..sup.8-tetrahydrocannabinol (.DELTA..sup.8THC) and
.DELTA..sup.9-tetrahydrocannabinol (.DELTA..sup.9THC). In
alternative embodiments, provided are processes for converting
.DELTA..sup.8-THC to .DELTA..sup.9-THC.
[0010] In alternative embodiments, provided are processes and
methods for obtaining or purifying a substantially pure
.DELTA..sup.9 THC from a natural or a synthetic source, wherein
optionally the natural source comprises a plant or a microbial
material, or a material derived from a plant source,
comprising:
[0011] (a) obtaining or being provided a first extract, aliquot or
sample of the natural or synthetic source comprising a cannabidiol
(CBD) and/or .DELTA..sup.9 THC and/or A.sup.s THC and/or other
cannabinoids from the natural or the synthetic source;
[0012] (b) dissolving the first extract aliquot or sample in a
first solvent, or diluting the first extract aliquot or sample in a
first solvent;
[0013] (c) loading the solvent-dissolved or diluted first extract
aliquot or sample onto a first chromatography column, wherein
optionally the chromatography column is a normal phase
chromatography column, an ion exchange chromatography column, or a
reverse phase chromatography column,
[0014] wherein the .DELTA..sup.9 THC and/or .DELTA..sup.8 THC
and/or other cannabinoids are reversibly bound to the first
chromatography column,
[0015] wherein optionally some of or substantially most of the
extract aliquot or sample material not reversibly bound to the
first column at the first station is removed with a first wash
solution before the column is moved to a next or a second
station;
[0016] (d) eluting the .DELTA..sup.9 THC and/or .DELTA..sup.8 THC
and/or other cannabinoids off the first chromatography column with
an elution solvent mixture, wherein optionally the eluting is a
gradient elution;
[0017] (e) collecting or isolating the .DELTA..sup.9 THC and/or
.DELTA..sup.8 THC and/or other cannabinoids from said elution
solvent mixture,
[0018] wherein optionally the elution is by a gradient elution
process and the .DELTA..sup.9 THC and/or .DELTA..sup.8 THC and/or
other cannabinoids are separately collected in different or
separate gradient elution fractions,
[0019] wherein optionally, if the elution is by a gradient elution
process using normal phase column chromatography: in a first
gradient polar compounds and some or substantially most of the
cannabinol (CBN), cannabigerol (CBG), cannabichromene (CBC) are
eluted off the column; in a second gradient solvent remaining
cannabidiol (CBD) is eluted off the column; in a third gradient a
CBD/tetrahydrocannabinol (THC) mix is first eluted and then
remaining THC is eluted off the column; in a fourth gradient
remaining cannabinoids are eluted off the column, wherein
optionally the remaining cannabinoids comprise CBC, CBG, CBN; and,
in a fifth gradient solvent all remaining polar compounds are
eluted off the column, wherein the remaining polar compounds
comprises cannabidiolic acid (CBDA) and tetrahydrocannabinolic acid
(THCA),
[0020] and optionally, if the elution is by a gradient elution
process using reverse phase column chromatography, the gradient
elution: [0021] begins with an 80:20 or a 70:30 methanol to water
elution solution or equivalent, which elutes off the column some or
substantially most of the polar compounds, wherein optionally the
polar compounds comprise CBDA and/or TGCA; [0022] then the gradient
elution moves to an 85:15 or an 80:20 methanol to water elution
solvent or equivalent, which elutes off the column some or
substantially most of the CBD; [0023] then the gradient elution
moves to a 90:10 methanol to water elution solution or equivalent,
which initially elutes off the column a mix of the remaining CBD
and THC, and finally elutes off the column THC; and [0024] finally
the gradient elution moves to a 100% methanol elution solution,
which removes a mix of remaining cannabinoids comprising CBC, CBG,
CBN;
[0025] (f) adding a second solvent of opposite polarity (to the
elution solvent mixture) to said elution solvent mixture;
[0026] (g) removing all or substantially most of the second
solvent, thereby leaving a first extract comprising a plurality of
cannabinoids comprising the .DELTA..sup.9 THC and/or .DELTA..sup.8
THC and/or other cannabinoids, wherein optionally the other
cannabinoids comprise CBC, CBG, CBN;
[0027] (h) dissolving the first extract comprising the plurality of
cannabinoids in a first reaction solvent;
[0028] (i) adding a catalyst to the first extract comprising the
plurality of cannabinoids and the first reaction solvent, wherein
the catalyst can be added before, simultaneous with or after
addition of the first reaction solvent to the extract comprising
the plurality of cannabinoids; and
[0029] (j) reacting the first extract comprising the plurality of
cannabinoids, the catalyst and the first reaction solvent,
[0030] wherein optionally the reaction converts .DELTA..sup.8-THC
to .DELTA..sup.9-THC;
[0031] (k) adding a neutralizing agent;
[0032] (l) removal of the catalyst and the neutralizing agent to
generate a first reaction product;
[0033] (m) optionally adding a second reaction solvent to the first
reaction product;
[0034] (n) optionally removing all or substantially most of the
second reaction solvent, thereby leaving a second extract
comprising a plurality of cannabinoids;
[0035] (o) adding a stabilization agent to the second extract;
[0036] (p) adding an elimination agent to the second extract;
[0037] (q) removal (or removal of substantially all) of the
stabilization agent and the elimination agent, thereby leaving a
third extract;
[0038] (r) and if the second reaction solvent is not removed in
step (n), adding a third solvent of opposite polarity to the third
extract;
[0039] (s) removing the second reaction solvent, if present (if not
removed in step (n)), and removing all or substantially most of the
third solvent of opposite polarity, if added, thereby leaving a
fourth extract;
[0040] (t) dissolving third or the fourth extract in a second
solvent, wherein optionally the second solvent is the same or
substantially the same as the first solvent;
[0041] (u) loading the dissolved third or the fourth extract onto a
second chromatography column, wherein optionally the chromatography
column is a normal phase chromatography column or a reverse phase
chromatography column,
[0042] wherein the plurality of cannabinoids are reversible bound
to the second chromatography column, and optionally the second
chromatography column is the same as the first chromatography
column;
[0043] (v) eluting the dissolved third or the fourth extract from
the second column with an elution solution,
[0044] wherein optionally the elution is by a gradient elution
process and the .DELTA..sup.9 THC and/or .DELTA..sup.8 THC and/or
other cannabinoids are separately collected in different or
separate gradient elution fractions;
[0045] (w) optionally collecting the plurality of cannabinoids from
the elution solution, and/or removing all or substantially most of
the elution solvent,
[0046] thereby obtaining or purifying a substantially pure
plurality of cannabinoids, wherein optionally the plurality of
cannabinoids comprises .DELTA..sup.9 THC and/or .DELTA..sup.8
THC.
[0047] In alternative embodiments, the CBD content of the first
extract, aliquot or sample material is greater than about 25%, 50%,
75%, 90% or 95% by weight, or is between about 5% and 95% by
weight, by weight of the material.
[0048] In alternative embodiments, the first solvent and/or the
second solvent comprises methanol, propanol, ethanol, isobutanol,
butanol, isopropanol, tetrahydrofuran, chloroform, acetone, hexane,
methylene dichloride, and/or dichloroethane; and optionally the
first solvent and/or the second solvent comprises chloroform,
methylene dichloride and methanol, propanol and/or ethanol.
[0049] In alternative embodiments, the chromatography column, e.g.,
a normal phase chromatography column, ion exchange column or
reverse phase column, comprises a silica gel, an alumina or an ion
exchange material, and optionally the ion exchange column comprises
an anion exchange material.
[0050] In alternative embodiments, the elution solution comprises
or is a solvent, and the solvent comprises a one or a mixture of:
ethylene dichloride; chloroform; or methylene dichloride; and
alcohol comprising methanol, ethanol, propanol or butanol or a
mixture thereof; an acid comprising acetic acid, formic acid,
oxalic acid, glycolic acid or a mixture thereof; and/or, ammonium
hydroxide. In alternative embodiments, the elution solvent is or
comprises a dichloromethane, dichloroethane, or chloroform and
methanol or ethanol.
[0051] In alternative embodiments, the first reaction solvent
comprises ethylene dichloride, chloroform, methylene dichloride,
toluene, xylene, benzene, hexane and/or pentane, or mixtures
thereof.
[0052] In alternative embodiments, the catalyst is or comprises a
Lewis acid or a non-oxidizing acid catalyst. In alternative
embodiments, the catalyst comprises p-toluenesulfonic acid.
[0053] In alternative embodiments, the neutralizing agent is or
comprises an alkali. In alternative embodiments, the neutralizing
agent is or comprises sodium bicarbonate, sodium carbonate, calcium
hydroxide or ammonium hydroxide.
[0054] In alternative embodiments, the stabilization agent is or
comprises zinc chloride or methylene chloride.
[0055] In alternative embodiments, the second reaction solvent
comprises dimethyl sulfoxide, water, dimethylformamide, methanol,
ethylene dichloride, chloroform, propanol, ethanol, isobutanol,
formamide, methylene dichloride, butanol, isopropanol,
tetrahydrofuran, dioxane, benzene, toluene and/or xylene or a
mixture thereof In alternative embodiments, the second reaction
solvent comprises chloroform, hexane, dichloromethane,
dichloroethane, benzene or toluene or a mixture thereof.
[0056] In alternative embodiments, the elimination agent is or
comprises a strong base; and optionally the strong base comprises:
lithium hydroxide (LiOH), sodium hydroxide (NaOH), potassium
hydroxide (KOH), rubidium hydroxide (RbOH), cesium hydroxide
(CsOH), magnesium hydroxide (Mg(OH).sub.2), calcium hydroxide
(Ca(OH).sub.2), strontium hydroxide Sr(OH).sub.2), barium hydroxide
Ba(OH).sub.2), potassium hydride and/or sodium hydride, potassium
tert-pentoxide, organic superbases, bi spi dines, multi cyclic
polyamines, organometallic compounds of reactive metals, wherein
optionally the reactive metals comprise organolithium,
organo-magnesium, lithium diisopropylamide, n-butyl lithium and
potassium tert-butoxide, sodium methoxide, or sodium ethoxide. In
alternative embodiments, the elimination agent is or comprises
potassium tert-pentoxide, lithium diisopropylamide and/or potassium
tert-butoxide.
[0057] In alternative embodiments, the elution solvent is or
comprises a solvent or mixture of solvents, e.g., as described in
the Examples.
[0058] In alternative embodiments, the extract is filtered before
being loaded on to the column, and optionally the extract is mixed
with silica, diatomaceous earth (DE), bentonite clay (BC), or
equivalents, or mixtures thereof, before filtering, and optionally
the extract is chilled before being mixed with the silica, DE, BC
or equivalents or is chilled before being filtered.
[0059] In alternative embodiments, the microbial material comprises
or is derived from a bacterium, an algal cell, a lichen or a yeast,
or a recombinant bacterium, lichen, algal cell or yeast cell.
[0060] In alternative embodiments, the plant or starting material
comprises or is derived from a botanical drug substance, including
hemp or a cannabis plant, wherein optionally the cannabis plant is
a Cannabis sativa, a Cannabis chemovar, or variants thereof, or a
decarboxylated Cannabis plant material. In alternative embodiments,
the plant or starting material comprises oils or extracts from a
trichome or a trichome fraction of a pubescent plant, or an algae
or a lichen; and optionally the oils or extracts are from the plant
or microbial material by: washing, contacting or exposing the
trichome or trichome fraction, or the pubescent plant, algae or
lichen, with: at least one non-polar, organic solvent; at least one
polar, organic solvent; or, a mix of at least one non-polar,
organic solvent with (and) at least one polar, organic solvent.
Chapt. II
[0061] In alternative embodiments, provided are methods of or
processes for purifying a cannabinoid to substantially pure
.DELTA..sup.9tetrahydrocannabinol (.DELTA..sup.9-THC) from a
natural or a synthetic source, wherein optionally the natural
source comprises a plant or microbial material, or a material
derived from a plant or a microbial source.
[0062] In one embodiment, the method comprises:
[0063] a. providing or having provided a first extract, aliquot or
sample of the natural or synthetic source comprising at least one
of cannabidiol (CBD), .DELTA..sup.9 THC, .DELTA..sup.8 THC, and
other cannabinoids from the natural or the synthetic source,
optionally a plant material;
[0064] b. providing or having provided a continuous chromatography
apparatus or device comprising a plurality of stations and a
plurality of chromatography columns, wherein optionally the
plurality of stations and the plurality of chromatography columns
comprise at least 2, 3, 4, 5, 6, 7, or 8 or more, or between about
3 and 30, stations and/or chromatography columns;
[0065] c. introducing or loading the first extract, aliquot or
sample into a first column at a first station, wherein the CBD,
.DELTA..sup.9 THC, and .DELTA..sup.8 THC and other cannabinoids
reversibly bind to the first column,
[0066] wherein optionally some of or substantially most of the
first extract material not reversibly bound to the first column at
the first station is removed with a first wash solution before the
column is moved to a next or a second station;
[0067] d. moving the first column to a second station, wherein
after the first column is moved to the second station a next or
second column is introduced into the first station;
[0068] e. eluting the extract from the first chromatography column
with a first elution solvent at the second station, wherein
optionally the eluting is a gradient elution;
[0069] f. collecting the first elution fractions from the first
column at the second station;
[0070] g. removing all or substantially most of the first elution
solution from the first elution fractions to produce a first
purified extract substantially free of CBD, .DELTA..sup.9 THC, and
.DELTA..sup.8 THC;
[0071] h. optionally repeating steps d to g on the first column
with at least one additional elution solvent, wherein the at least
one additional elution solvent produces a second purified extract
substantially free of CBD, .DELTA..sup.9 THC, and .DELTA..sup.8
THC;
[0072] i. when the first column is moved to the next, or second,
station, introducing a next or second column into the first
station, and the extract, aliquot or sample is introduced or
loading into the next or second column at the first station;
[0073] j. moving the second column to the next or second station
after the first column is moved to a next or third station;
[0074] k. eluting the extract from the first column with a second
elution solvent at the next or second station,
[0075] wherein optionally the elution is by a gradient elution
process and the CBD, .DELTA..sup.9 THC, and .DELTA..sup.8 THC and
other cannabinoids are separately collected in different or
separate gradient elution fractions
[0076] wherein optionally, if the elution is by a gradient elution
process: in a first gradient solvent non-polar compounds and
terpenes are eluted off the column; in a second gradient solvent
cannabidiol (CBD) is eluted off the column; in a third gradient
solvent a CBD/tetrahydrocannabinol (THC) mix is first eluted and
then remaining THC is eluted off the column; in a fourth gradient
minor cannabinoids are eluted off the column, wherein optionally
the minor cannabinoids comprise CBC, CBG, CBN; and, in a fifth
gradient solvent all remaining polar compounds are eluted off the
column, wherein the remaining polar compounds comprises
cannabidiolic acid (CBDA) and tetrahydrocannabinolic acid
(THCA);
[0077] l. collecting the second elution fractions from the first
chromatography column at the second station;
[0078] m. removing all or substantially most of the second elution
solvent from the second elution fractions to produce a second
purified extract (or a third purified extract if step h is
completed) comprising at least about 75% of the CBD, .DELTA..sup.9
THC, and .DELTA..sup.8 THC from the first extract, or between about
60% and 90% of CBD, .DELTA..sup.9 THC, and .DELTA..sup.8 THC, or
between about 50% and 95% of CBD, .DELTA..sup.9 THC, and
.DELTA..sup.8 THC,
[0079] and optionally the gradient elution: [0080] begins with an
80:20 or a 70:30 methanol to water elution solution or equivalent,
which elutes off the column some or substantially most of the
cannabidiolic acid (CBDA) and tetrahydrocannabinolic acid (THCA)
and polar terpenes; [0081] then the gradient elution moves to an
85:15 an 80:20 methanol to water elution solvent or equivalent,
which elutes off the column some or substantially most of the CBD;
[0082] then the gradient elution moves to a 90:10 methanol to water
elution solution or equivalent, which initially elutes off the
column a mix of the remaining CBD and THC, and finally elutes off
the column THC; and [0083] finally the gradient elution moves to a
100% methanol elution solution, which removes a mix of remaining
cannabinoids comprising CBC, CBG, CBN;
[0084] n. optionally repeating steps j to m on the first column
with at least one second additional elution solution;
[0085] o. optionally repeating steps i to m with at least one
additional column;
[0086] p. optionally mixing the second purified extract with a
reaction mixture comprising a reaction solvent and a catalyst,
wherein the reaction mixture comprises an aqueous phase and an
organic phase;
[0087] q. optionally separating the mixture into an aqueous phase
and an organic phase (separating the aqueous phase and the organic
phase): and
[0088] r. optionally recovering the organic phase to obtain the
substantially pure .DELTA..sup.9-THC.
[0089] In some embodiments, the method further comprises: adding a
neutralizing agent to the reaction mixture; and removing all or
substantially most of the catalyst and the neutralizing agent
before separating the reaction mixture.
[0090] In some embodiments, the method further comprises: adding at
least one of a stabilization agent and an elimination agent to the
reaction mixture; and removing all or substantially most of the at
least one of the stabilization agents and the elimination agent
before separating the reaction mixture.
[0091] In some embodiments, the extract, aliquot or sample
comprises at least 25% by weight CBD, or between about 10% to 40%
by weight CBD, or between about 5% to 50% by weight CBD.
[0092] In some embodiments, at least one of the first elution
solvent, the additional elution solvent, the second elution
solvent, and the second additional elution solvent comprises
methanol, ethanol, propanol, butanol, chloroform, dichloromethane,
dichloroethane, acetic acid, formic acid, oxalic acid, glycolic
acid, ammonium hydroxide, or combinations thereof.
[0093] In some embodiments, the reaction solvent comprises ethylene
dichloride, chloroform, methylene dichloride, toluene, xylene,
benzene, hexane, pentane, or combinations thereof.
[0094] In some embodiments, the catalyst comprises a Lewis acid or
a non-oxidizing acid. In some embodiments, the catalyst comprises
p-toluenesulfonic acid
[0095] In some embodiments, the neutralizing agent comprises an
alkali. In some embodiments, the neutralizing agent comprises
sodium bicarbonate, sodium carbonate, calcium hydroxide, or
ammonium hydroxide, or combinations thereof.
[0096] In some embodiments, the stabilization agent is or comprises
zinc chloride or methylene chloride, or combinations thereof.
[0097] In some embodiments, the method further comprises: adding a
second reaction solvent to the organic phase after the mixture is
separated.
[0098] In some embodiments, the second reaction solvent comprises
dimethyl sulfoxide, pyridine, dimethylformamide, methanol, ethylene
dichloride, chloroform, propanol, ethanol, isobutanol, formamide,
methylene dichloride, butanol, isopropanol, tetrahydrofuran,
dioxane, benzene, toluene, xylene, or combinations thereof.
[0099] In some embodiments, the elimination agent comprises lithium
hydroxide (LiOH), sodium hydroxide (NaOH), potassium hydroxide
(KOH), rubidium hydroxide (RbOH), cesium hydroxide (CsOH),
magnesium hydroxide (Mg(OH).sub.2), calcium hydroxide
(Ca(OH).sub.2), strontium hydroxide Sr(OH).sub.2), barium hydroxide
Ba(OH).sub.2), potassium hydride and/or sodium hydride, potassium
tert-pentoxide, organic superbases, bispidines, multicyclic
polyamines, or organometallic compounds of reactive metals, wherein
optionally the reactive metals comprise organolithium,
organomagnesium, lithium diisopropylamide, n-butyl lithium and
potassium tert-butoxide, sodium methoxide, or sodium ethoxide,
lithium nitride, potassium hydride, sodium hydride, or combinations
thereof.
[0100] In some embodiments, the method further comprises:
dissolving, filtering and/or diluting the extract, aliquot or
sample in a first solvent before introducing or loading the
extract, aliquot or sample into the first chromatography column, or
the column at the first station, which can be a second, third,
fourth etc. chromatography column.
[0101] In some embodiments, the first solvent comprises methanol,
ethanol, propanol, isobutanol, butanol, isopropanol,
tetrahydrofuran, chloroform, methylene dichloride, di chloroethane,
or combinations thereof.
[0102] In some embodiments, the method further comprises: filtering
the extract, sample or aliquot to remove all or substantially most
of the solids and color bodies in the natural or synthetic material
before introducing or loading the extract, aliquot or sample into
the first column, or any additional column at the first
station.
[0103] In some embodiments, at least one of the first
chromatography column, the second chromatography column, or the at
least one additional chromatography column comprises a normal phase
chromatography column, a reverse phase chromatography column, or an
ion exchange chromatography column.
[0104] In some embodiments, the method further comprises measuring
the amount of .DELTA..sup.9-THC, or other cannabinoid, in the
organic phase.
[0105] In some embodiments, the first and second elution solutions
are different.
[0106] In some embodiments, there are at least 4 columns, at least
four elution solvents, and at least four purified extracts, or
between about 3 and 30 elution solvents and purified extracts. In
some embodiments, the at least four elution solvents are
different.
[0107] In alternative embodiments, methods and processes comprise a
continuous column chromatography process for isolating and/or
modifying cannabidiol (CBD) and/or .DELTA..sup.9 THC and/or
.DELTA..sup.8 THC and/or other cannabinoids, comprising:
[0108] (a) at a first station, loading onto a first column the
extract, aliquot or sample of the natural or synthetic source
comprising a cannabidiol (CBD) and/or .DELTA..sup.9 THC and/or
.DELTA..sup.8 THC and/or other cannabinoids from the natural or the
synthetic source, and then moving the first column to a second
station;
[0109] (b) at the second station, introducing into the first column
an elution solvent comprising an 80:20 or a 70:30 methanol to water
elution solution or equivalent, which elutes off the first column
some or substantially most of the polar compounds and terpenes
comprising cannabidiolic acid (CBDA) and tetrahydrocannabinolic
acid (THCA), and then moving the first column to a third
station;
[0110] (c) at the third station, introducing into the first column
an elution solvent comprising an 85:15 or an 80:20 methanol to
water elution solvent or equivalent, which elutes off the first
column some or substantially most of the CBD, and then moving the
first column to a fourth station;
[0111] (d) at the fourth station, introducing into the first column
an elution solvent comprising a 90:10 methanol to water elution
solution or equivalent, which elutes off the first column the minor
CBD, and some THC, and finally elutes off the column more or
substantially most of the THC, then moving the first column to a
fifth station;
[0112] (e) at the fifth station, introducing into the first column
an elution solution comprising a 100% methanol elution solution,
which removes a mix of remaining cannabinoids comprising CBC, CBG,
CBN, then moving the first column to a sixth station;
[0113] (f) at the sixth station, introducing into the first column
an elution solution comprising dichloromethane (DCM), chloroform or
equivalent to remove or elute off the non-polar compounds
reversibly bound to the column, wherein optionally the non-polar
compounds comprise cannabidiol (CBD), cannabinol (CBN),
cannabigerol (CBG), cannabichromene (CBC), cannabidivarol (CBDV),
tetrahydrocannabidiol (THCBD), tetrahydrocannabigerol (THCBG),
tetrahydrocannabichromene (THCBC), or tetrahydrocannabidivarol
(THCBDV), then moving the first column to a seventh station or back
to the first station; and
[0114] (g) introducing into the first column an 80:20 or a 70:30
methanol to water elution solution or equivalent either at the
seventh station or the first station in an amount sufficient to
remove all or substantially most of the dichloromethane (DCM),
chloroform or equivalent, and if the 80:20 or the 70:30 methanol to
water elution solvent or equivalent is introduced at the seventh
station, after remove all or substantially most of the
dichloromethane (DCM), chloroform or equivalent the first column is
moved back to the first station;
[0115] wherein one or several fractions are collected at each
station, or at least at the first to fifth stations or first to
sixth stations.
[0116] In alternative embodiments, the method or processes further
comprise mixing the isolated cannabidiol (CBD) and/or .DELTA..sup.9
THC and/or .DELTA..sup.8 THC and/or other cannabinoid fractions
with a reaction solvent and a catalyst, for example, for converting
cannabidiol (CBD) to .DELTA..sup.8-THC and/or .DELTA..sup.9-THC, or
for converting .DELTA..sup.8-THC to .DELTA..sup.9-THC.
[0117] In alternative embodiments, provided are methods of
purifying a cannabinoid to substantially pure .DELTA..sup.9-THC
from a natural or a synthetic source, e.g., a plant or a microbial
material, comprising:
[0118] a. providing an extract, aliquot or sample of the natural or
synthetic source comprising at least one of cannabidiol (CBD),
.DELTA..sup.9 THC, THC, and other cannabinoids from the natural or
a synthetic source, wherein the natural or a synthetic source
comprises a plant or a microbial material;
[0119] b. loading the CBD-, .DELTA..sup.9 THC-, .DELTA..sup.8
THC-comprising extract, aliquot or sample on a chromatography
column,
[0120] wherein optionally the chromatography column is a normal
phase chromatography column, an ion exchange chromatography column,
or a reverse phase chromatography column, and
[0121] eluting the column with a first elution solution so that all
or substantially most of the CBD, .DELTA..sup.9 THC, .DELTA..sup.8
THC remains in (or reversibly bound to) the column and the all or
substantially most of the remaining natural or synthetic,
optionally plant or microbial, material is eluted out of the
column;
[0122] c. collecting the eluted material and removing all or
substantially most of the first elution solution, wherein the first
elution solution is substantially free of CBD, .DELTA..sup.9 THC,
.DELTA..sup.8 THC;
[0123] d. eluting some or substantially most of the CBD,
.DELTA..sup.9 THC and/or .DELTA..sup.8 THC from the column with a
second elution solution, wherein optionally between about 50% and
99% of the CBD, .DELTA..sup.9 THC and/or .DELTA..sup.8 THC is
eluted from the column, and collecting the CBD, .DELTA..sup.9 THC,
.DELTA..sup.8 THC, or collecting the CBD, .DELTA..sup.9 THC,
.DELTA..sup.8 THC elution fractions, wherein one or multiple
fractions are collected, and optionally the CBD, .DELTA..sup.9 THC,
.DELTA..sup.8 THC are collected in separate elution fractions,
and
[0124] removing all or substantially most of the second elution
solution,
[0125] wherein optionally the elution of step (d) is by a gradient
elution process,
[0126] and optionally the elution is by a gradient elution process
and the .DELTA..sup.9 THC and/or .DELTA..sup.8 THC and/or other
cannabinoids are separately collected in different or separate
gradient elution fractions,
[0127] wherein optionally, if the elution is by a gradient elution
process: in a first gradient solvent non-polar compounds and
terpenes are eluted off the column; in a second gradient solvent
cannabidiol (CBD) is eluted off the column; in a third gradient a
CBD/tetrahydrocannabinol (THC) mix is first eluted and then
remaining THC is eluted off the column; in a fourth gradient minor
cannabinoids are eluted off the column, wherein optionally the
minor cannabinoids comprise CBC, CBG, CBN; and, in a fifth gradient
solvent all remaining polar compounds are eluted off the column,
wherein the remaining polar compounds comprises cannabidiolic acid
(CBDA) and tetrahydrocannabinolic acid (THCA);
[0128] e. loading the collected CBD, .DELTA..sup.9 THC,
.DELTA..sup.8 THC on a second chromatography column and eluting the
CBD from the second column with a third elution solution (the
.DELTA..sup.9 THC and .DELTA..sup.8 THC stay on the second column),
collecting the second eluted material containing (or comprising)
the CBD, and removing all or substantially most of the third
elution solvent;
[0129] f. eluting the .DELTA..sup.9 THC and .DELTA..sup.8 THC from
the second column with a fourth elution solution, collecting the
fraction, and removing all or substantially most of the fourth
elution solution;
[0130] g. adding the CBD from the second column to a reaction
solvent and adding a catalyst forming a reaction mixture, wherein
the reaction mixture comprises an aqueous phase and an organic
phase.
[0131] In some embodiments, steps b to d are continuously repeated
and wherein steps e to g are continuously repeated.
[0132] In some embodiments, the method further comprises: adding a
neutralizing agent to the mixture; and removing all or
substantially most of the catalyst and the neutralizing agent
before separating the mixture, and optionally separating the
aqueous phase from the organic phase.
[0133] In some embodiments, the method further comprises: adding at
least one of a stabilization agent and an elimination agent to the
mixture; and removing all or substantially most of the at least one
of the stabilization agent and the elimination agent before
separating the mixture.
[0134] In some embodiments, the extract comprises at least 25% CBD
by weight, or between about 10% to 40% CBD by weight, or between
about 5% to 50% CBD by weight, by weight.
[0135] In some embodiments, at least one of the first elution
solvent, the second elution solvent, the third elution solvent, and
the fourth additional elution solvent comprises methanol, ethanol,
propanol, butanol, chloroform, dichloromethane, dichloroethane,
acetic acid, formic acid, oxalic acid, glycolic acid, ammonium
hydroxide, water, or combinations thereof.
[0136] In some embodiments, the reaction solvent comprises ethylene
dichloride, chloroform, methylene dichloride, toluene, xylene,
benzene, hexane, pentane, water, or combinations thereof.
[0137] In some embodiments, the catalyst comprises a Lewis acid or
a non-oxidizing acid. In some embodiments, the catalyst comprises
p-toluenesulfonic acid
[0138] In some embodiments, the neutralizing agent comprises an
alkali. In some embodiments, the neutralizing agent comprises
sodium bicarbonate, sodium carbonate, calcium hydroxide or ammonium
hydroxide.
[0139] In some embodiments, the stabilization agent is or comprises
zinc chloride or methylene chloride.
[0140] In some embodiments, the method further comprises: adding a
second reaction solvent to the organic phase after the mixture is
separated.
[0141] In some embodiments, the second reaction solvent comprises
dimethyl sulfoxide, pyridine, water, dimethylformamide, methanol,
ethylene dichloride, chloroform, propanol, ethanol, isobutanol,
formami de, methylene dichloride, butanol, isopropanol,
tetrahydrofuran, dioxane, benzene, toluene, xylene, or combinations
thereof.
[0142] In some embodiments, the elimination agent comprises lithium
hydroxide (LiOH), sodium hydroxide (NaOH), potassium hydroxide
(KOH), rubidium hydroxide (RbOH), cesium hydroxide (CsOH),
magnesium hydroxide (Mg(OH).sub.2), calcium hydroxide
(Ca(OH).sub.2), strontium hydroxide Sr(OH).sub.2), barium hydroxide
Ba(OH).sub.2), potassium hydride and/or sodium hydride, potassium
tert-pentoxide, organic superbases, bispidines, multicyclic
polyamines, organometallic compounds of reactive metals, wherein
optionally the reactive metals comprise organolithium,
organomagnesium, lithium diisopropylamide, sodium methoxide, or
sodium ethoxide, or combinations thereof.
[0143] In some embodiments, the method further comprises:
dissolving, diluting and/or filtering the extract, aliquot or
sample comprising the natural or synthetic material in a first
solvent before introducing or loading the extract, aliquot or
sample into the first column, or before the initial loading of the
second or additional columns.
[0144] In some embodiments, the first solvent comprises methanol,
ethanol, propanol, isobutanol, butanol, isopropanol,
tetrahydrofuran, chloroform, methylene dichloride, dichloroethane,
water, or combinations thereof.
[0145] In some embodiments, the method further comprises: filtering
the extract, aliquot or sample comprising the natural or synthetic
material to remove all or substantially most of the material's
solids and color bodies before introducing or loading the extract,
aliquot or sample into the first column, or before the initial
loading of the extract, aliquot or sample in the second or
additional columns.
[0146] In some embodiments, at least one of the columns, or the
first and/or the second column comprises a normal phase
chromatography column, a reverse phase chromatography column, or an
ion exchange chromatography column. In alternative embodiments, all
of the columns are the same (e.g., all are normal phase
chromatography columns or reverse phase chromatography columns), or
the columns differ (e.g., some are normal phase chromatography
columns and some are reverse phase chromatography columns or ion
exchange chromatography columns).
[0147] In some embodiments, the method further comprising measuring
the amount of .DELTA..sup.9-THC, or other cannabinoid, in the
organic phase.
[0148] In some embodiments, the first, second, third, and fourth
elution solutions are different.
[0149] In alternative embodiments, the natural or synthetic
material, or the extract, aliquot or sample, is filtered before
being loaded on to the column, and optionally the natural or
synthetic material, or the extract, aliquot or sample, is mixed
with silica, diatomaceous earth (DE), bentonite clay (BC), or
equivalents, or mixtures thereof, before filtering, and optionally
the natural or synthetic material, or the extract, aliquot or
sample, is chilled before being mixed with the silica, DE, BC or
equivalents or is chilled before being filtered.
[0150] In alternative embodiments, the microbial material comprises
or is derived from a bacterium, an algal cell, a lichen or a yeast,
or a recombinant bacterium, algal cell, lichen or yeast cell. In
alternative embodiments, the plant material comprises or is derived
from a botanical drug substance, including a hemp or a cannabis
plant, wherein optionally the cannabis plant is a Cannabis sativa,
a Cannabis chemovar, or variants thereof, or a decarboxylated
Cannabis plant material. In alternative embodiments, the plant or
starting material comprises oils or extracts from a trichome or a
trichome fraction of a pubescent plant, or an algae or a lichen;
and optionally the oils or extracts are from the plant or microbial
material by: washing, contacting or exposing the trichome or
trichome fraction, or the pubescent plant, algae or lichen, with:
at least one non-polar, organic solvent; at least one polar,
organic solvent; or, a mix of at least one non-polar, organic
solvent with (and) at least one polar, organic solvent.
Chapt. III
[0151] In alternative embodiments, provided are methods of and
processes for isolating or purifying a cannabinoid or a
substantially pure .DELTA..sup.8-THC from a natural or a synthetic
source, e.g., a plant or a microbial material.
[0152] In one embodiment, the method comprises:
[0153] a. providing an extract, aliquot or sample of the natural or
synthetic source comprising at least one of: cannabidiol (CBD),
.DELTA..sup.9 THC, .DELTA..sup.8 THC, and other cannabinoids from
the natural or a synthetic source, wherein the natural source
comprises a plant or a microbial material;
[0154] b. providing a continuous chromatography apparatus
comprising a plurality of stations and a plurality of
chromatography columns, wherein optionally the plurality of
stations and the plurality of chromatography columns comprise at
least 2, 3, 4, 5, 6, 7, or 8 or more, or between about 3 and 30,
stations and/or chromatography columns;
[0155] c. introducing or loading the extract, aliquot or sample of
the natural or synthetic source into a first column at a first
station, wherein CBD, .DELTA..sup.9 THC, and .DELTA..sup.8 THC
reversibly binds to the first column;
[0156] d. moving the first column to a next or second station;
[0157] e. eluting the extract from the first chromatography column
with a first elution solvent at the next or second station, wherein
the first elution solvent does not elute the CBD, .DELTA..sup.9
THC, and .DELTA..sup.8 THC from the first column;
[0158] f. collecting the first elution fractions from the first
column;
[0159] g. removing all or substantially most of the first elution
solution from the first elution fractions to produce a first
purified extract substantially free of CBD, .DELTA..sup.9 THC, and
.DELTA..sup.8 THC;
[0160] h. optionally repeating steps d to g with at least one
additional first elution solution;
[0161] i. when the first column is moved to the next or second
station, introducing or loading the extract into a next or second
column at the first station;
[0162] j. moving the second column to the next or second station
after the first column has been moved to a next or third
station;
[0163] k. eluting the extract, or material reversibly bound to the
first column with a second elution solution at the next or second
station to generate one or more second elution fractions, wherein
optionally the second elution solution comprises an organic
solvent;
[0164] l. collecting the second elution fractions from the second
chromatography column at the second station;
[0165] m. removing all or substantially most of the second elution
solution from the second elution fractions to produce a second
purified extract comprising at least about 75% of CBD,
.DELTA..sup.9 THC, and .DELTA..sup.8 THC, or between about 60% and
90% of CBD, .DELTA..sup.9 THC, and .DELTA..sup.8 THC, or between
about 50% and 95% of CBD, .DELTA..sup.9 THC, and .DELTA..sup.8
THC,
[0166] wherein optionally the elution is by a gradient elution
process and the .DELTA..sup.9 THC and/or .DELTA..sup.8 THC and/or
other cannabinoids are separately collected in different or
separate gradient elution fractions,
[0167] wherein optionally, if the elution is by a gradient elution
process: in a first gradient solvent non-polar compounds and
terpenes are eluted off the column; in a second gradient solvent
cannabidiol (CBD) is eluted off the column; in a third gradient a
CBD/tetrahydrocannabinol (THC) mix is first eluted and then
remaining THC is eluted off the column; in a fourth gradient
remaining cannabinoids are eluted off the column, wherein
optionally the remaining cannabinoids comprise CBC, CBG, CBN; and,
in a fifth gradient solvent all remaining polar compounds are
eluted off the column, wherein the remaining polar compounds
comprises cannabidiolic acid (CBDA) and tetrahydrocannabinolic acid
(THCA);
[0168] n. optionally repeating steps j to m with at least one
second additional elution solution;
[0169] o. optionally repeating steps i to m with at least one
additional column;
[0170] p. recovering the second purified extract, or recovering the
gradient elution fraction, comprising a substantially pure CBD and
the organic solvent, wherein optionally if a gradient elution is
used the CBD is in one or more elution fractions separate from one
or more of the other cannabinoids or polar compounds;
[0171] q. optionally removing all or substantially most of the
organic solvent if an organic solvent is used in the gradient
elution fraction;
[0172] r. optionally adding a second organic solvent to the second
purified extract;
[0173] s. adding at least one catalyst to the organic phase under
an inert atmosphere to catalyze the conversion of CBD to
.DELTA..sup.9-THC and a second order reaction of .DELTA..sup.9-THC
to .DELTA..sup.8-THC;
[0174] t. optionally controlling the reaction temperature to be in
a range of between about -10.degree. C. to about 40.degree. C. and
measuring the rate of reaction by observing the conversion of CBD
to .DELTA..sup.9-THC and second order reaction of .DELTA..sup.9-THC
to .DELTA..sup.8-THC;
[0175] u. adding a base to a first organic phase when the
.DELTA..sup.9-THC has been substantially converted to
.DELTA..sup.8-THC;
[0176] v. allowing the mixture to separate into an aqueous phase
and a second organic phase; and
[0177] w. recovering the second organic phase comprising the
converted .DELTA..sup.8-THC.
[0178] In some embodiments, the method further comprises repeating
steps s to w.
[0179] In some embodiments, the method further comprises: repeating
steps c to m and wherein the extract, aliquot or sample of the
natural or synthetic source in step c is replaced with the
.DELTA..sup.8-THC as recovered in step w.
[0180] In some embodiments, the extract, aliquot or sample of the
natural or synthetic source comprises at least 25% CBD by weight,
or between about 10% to 40% CBD by weight, or between about 5% to
50% CBD by weight.
[0181] In some embodiments, at least one of the first elution
solvent, the additional elution solvent, the second elution
solvent, and the second additional elution solvent comprises
methanol, ethanol, propanol, butanol, chloroform, dichloromethane,
dichloroethane, acetic acid, formic acid, oxalic acid, glycolic
acid, ammonium hydroxide, water, or combinations thereof.
[0182] In some embodiments, the catalyst comprises a Lewis acid or
a non-oxidizing acid. In some embodiments, the catalyst comprises
p-toluenesulfonic acid
[0183] In some embodiments, the base comprises sodium
bicarbonate.
[0184] In some embodiments, the method further comprises:
dissolving, dissolving and/or diluting the extract, aliquot or
sample of the natural or synthetic source in a first solvent before
introducing or loading the extract, aliquot or sample of the
natural or synthetic source into the first chromatography column,
or loading the extract, aliquot or sample into the second or
additional chromatography columns.
[0185] In some embodiments, the first solvent comprises methanol,
ethanol, propanol, isobutanol, butanol, isopropanol,
tetrahydrofuran, chloroform, methylene dichloride, dichloroethane,
water, or combinations thereof
[0186] In some embodiments, the method further comprises: filtering
the extract, aliquot or sample of the natural or synthetic source
to remove all or substantially most of the material's solids and
color bodies before introducing or loading the extract, aliquot or
sample of the natural or synthetic source into the first column, or
before the initial loading of the extract, aliquot or sample in the
second or additional columns.
[0187] In some embodiments, at least one of the first
chromatography column, the second chromatography column, or the at
least one additional chromatography column comprises a normal phase
chromatography column, a reverse phase chromatography column, or an
ion exchange chromatography column.
[0188] In some embodiments, at least one of the organic solvents
and the second organic solvent comprises methanol, ethanol,
propanol, isobutanol, butanol, isopropanol, tetrahydrofuran,
chloroform, methylene dichloride, dichloroethane, water, or
combinations thereof.
[0189] In some embodiments, the first and second elution solutions
are different.
[0190] In some embodiments, there are at least 4 columns, at least
four elution solutions, and at least four purified extracts.
[0191] In some embodiments, the at least four elution solvents are
different.
[0192] In alternative embodiments, the natural or synthetic
material, or the extract, aliquot or sample, is filtered before
being loaded on to the column, and optionally the natural or
synthetic material, or the extract, aliquot or sample, is mixed
with silica, diatomaceous earth (DE), bentonite clay (BC), or
equivalents, or mixtures thereof, before filtering, and optionally
the natural or synthetic material, or the extract, aliquot or
sample, is chilled before being mixed with the silica, DE, BC or
equivalents or is chilled before being filtered.
[0193] In alternative embodiments, the microbial material comprises
or is derived from a bacterium, an algal cell, a lichen or a yeast,
or a recombinant bacterium, algal cell, lichen or yeast cell.
[0194] In alternative embodiments, the plant material comprises or
is derived from a botanical drug substance, including a hemp or a
cannabis plant, wherein optionally the cannabis plant is a Cannabis
sativa, a Cannabis chemovar, or variants thereof, or a
decarboxylated Cannabis plant material. In alternative embodiments,
the plant or starting material comprises oils or extracts from a
trichome or a trichome fraction of a pubescent plant, or an algae
or a lichen; and optionally the oils or extracts are from the plant
or microbial material by: washing, contacting or exposing the
trichome or trichome fraction, or the pubescent plant, algae or
lichen, with: at least one non-polar, organic solvent; at least one
polar, organic solvent; or, a mix of at least one non-polar,
organic solvent with (and) at least one polar, organic solvent.
Chapt. IV
[0195] In alternative embodiments, provided are methods of or
processes for purifying cannabinoids from a natural or a synthetic
source, e.g., a plant or a microbial material.
[0196] In one embodiment, the method comprises:
[0197] a. providing an extract, aliquot or sample comprising at
least one of cannabidiol (CBD), .DELTA..sup.9 THC, .DELTA..sup.8
THC, and other cannabinoids from the natural or a synthetic source,
wherein optionally the natural or a synthetic source comprises or
is derived from a plant material;
[0198] b. providing a continuous chromatography apparatus
comprising a plurality of stations and a plurality of
chromatography columns, wherein optionally the plurality of
stations and the plurality of chromatography columns comprise at
least 2, 3, 4, 5, 6, 7, or 8 or more, or between about 3 and 30,
stations and/or chromatography columns;
[0199] c. introducing or loading the extract, aliquot or sample
into a first column at a first station;
[0200] d. moving the first column to a next or second station and a
second column to the first station;
[0201] e. eluting the extract, aliquot or sample from the first
chromatography column with a first elution solvent at the next or
second station,
[0202] wherein optionally the elution is by a gradient elution
process and the .DELTA..sup.9 THC and/or A.sup.8 THC and/or other
cannabinoids are separately collected in different or separate
gradient elution fractions,
[0203] wherein optionally, if the elution is by a gradient elution
process: in a first gradient solvent non-polar compounds and
terpenes are eluted off the column; in a second gradient solvent
cannabidiol (CBD) is eluted off the column; in a third gradient a
CBD/tetrahydrocannabinol (THC) mix is first eluted and then
remaining THC is eluted off the column; in a fourth gradient
remaining cannabinoids are eluted off the column, wherein
optionally the remaining cannabinoids comprise CBC, CBG, CBN; and,
in a fifth gradient solvent all remaining polar compounds are
eluted off the column, wherein the remaining polar compounds
comprises cannabidiolic acid (CBDA) and tetrahydrocannabinolic acid
(THCA);
[0204] f. collecting the first elution fractions from the first
column at the second station;
[0205] g. removing all or substantially most of the first elution
solution from the first elution fractions to produce a first
purified extract, wherein optionally the first purified extract
comprises THC, CBC, CBG, CBN, CBDA and/or THCA, and the THC, CBC,
CBG, CBN, CBDA and/or THCA are eluted separately in different
elution fractions to yield separate purified first extracts;
[0206] h. optionally repeating steps d to g with at least one
additional first or other elution solution;
[0207] i. when the first column is moved to the next or third
station, the second column is moved to the second station where
extract or reversibly bound material is eluted with the first
elution solution, and another batch of the extract, aliquot or
sample is introduced or loaded into a next or third column at the
first station;
[0208] j. moving the second column to the next or second
station;
[0209] k. eluting the extract from the second column with a first
elution solution at the next or second station;
[0210] l. collecting the second elution fractions from the second
column at the second station,
[0211] wherein optionally the elution is by a gradient elution
process and the .DELTA..sup.9 THC and/or .DELTA..sup.8 THC and/or
other cannabinoids are separately collected in different or
separate gradient elution fractions,
[0212] wherein optionally, if the elution is by a gradient elution
process: in a first gradient solvent non-polar compounds and
terpenes are eluted off the column; in a second gradient solvent
cannabidiol (CBD) is eluted off the column; in a third gradient a
CBD/tetrahydrocannabinol (THC) mix is first eluted and then
remaining THC is eluted off the column; in a fourth gradient
remaining cannabinoids are eluted off the column, wherein
optionally the remaining cannabinoids comprise CBC, CBG, CBN; and,
in a fifth gradient solvent all remaining polar compounds are
eluted off the column, wherein the remaining polar compounds
comprises cannabidiolic acid (CBDA) and tetrahydrocannabinolic acid
(THCA);
[0213] m. removing all or substantially most of the second elution
solution from the second elution fractions to produce a second
purified extract;
[0214] n. optionally repeating steps j to m with at least one
second additional elution solution; and
[0215] o. optionally repeating steps i to m with at least one
additional column.
[0216] In some embodiments, the extract comprises at least 25% CBD
by weight, or between about 10% to 40% CBD by weight, or between
about 5% to 50% CBD by weight.
[0217] In some embodiments, at least one of the first elution
solution, the additional elution solution, the second elution
solution, and the second additional elution solution comprises
methanol, ethanol, propanol, butanol, chloroform, dichloromethane,
dichloroethane, acetic acid, formic acid, oxalic acid, glycolic
acid, ammonium hydroxide, water, or combinations thereof.
[0218] In some embodiments, the method further comprises:
dissolving the extract in a first solvent before introducing the
extract into the first chromatography column or the second
chromatography column.
[0219] In some embodiments, the first solvent comprises methanol,
ethanol, propanol, isobutanol, butanol, isopropanol,
tetrahydrofuran, chloroform, methylene dichloride, dichloroethane,
water, or combinations thereof.
[0220] In some embodiments, at least one of the first
chromatography column, the second chromatography column, or the at
least one additional chromatography column comprises a normal phase
chromatography column, a reverse phase chromatography column, or an
ion exchange chromatography column.
[0221] In some embodiments, the first and second elution solutions
are different.
[0222] In some embodiments, there are at least 2, 3, 4, 5, 6, 7, or
8 or more columns, with at least 2, 3, 4, 5, 6, 7, or 8 or more
corresponding elution solvents, and at least 2, 3, 4, 5, 6, 7, or 8
or more corresponding purified extracts.
[0223] In some embodiments, the at least 2, 3, 4, 5, 6, 7, or 8 or
more elution solutions are different.
[0224] In some embodiments, the method further comprises measuring
the amount of the cannabinoid in the first or second purified
extract.
[0225] In alternative embodiments, the natural or synthetic
material, or the extract, aliquot or sample, is filtered before
being loaded on to the column, and optionally the natural or
synthetic material, or the extract, aliquot or sample, is mixed
with silica, diatomaceous earth (DE), bentonite clay (BC), or
equivalents, or mixtures thereof, before filtering, and optionally
the natural or synthetic material, or the extract, aliquot or
sample, is chilled before being mixed with the silica, DE, BC or
equivalents or is chilled before being filtered.
[0226] In alternative embodiments, the microbial material comprises
or is derived from a bacterium, an algal cell, a lichen or a yeast,
or a recombinant bacterium, algal cell, lichen or yeast cell.
[0227] In alternative embodiments, the plant material comprises or
is derived from a botanical drug substance, including a hemp or a
cannabis plant, wherein optionally the cannabis plant is a Cannabis
sativa, a Cannabis chemovar, or variants thereof, or a
decarboxylated Cannabis plant material. In alternative embodiments,
the plant or starting material comprises oils or extracts from a
trichome or a trichome fraction of a pubescent plant, or an algae
or a lichen; and optionally the oils or extracts are from the plant
or microbial material by: washing, contacting or exposing the
trichome or trichome fraction, or the pubescent plant, algae or
lichen, with: at least one non-polar, organic solvent; at least one
polar, organic solvent; or, a mix of at least one non-polar,
organic solvent with (and) at least one polar, organic solvent.
Chapt. V
[0228] In alternative embodiments, provided are methods of and
processes for purifying a cannabinoid from a natural or a synthetic
source, e.g., a plant or a microbial material, to a purified
mixture comprising .DELTA..sup.8tetrahydrocannabinol
(.DELTA..sup.8-THC) and .DELTA..sup.9tetrahydrocannabinol
(.DELTA..sup.9-THC).
[0229] In alternative embodiments, the method comprises:
[0230] a. providing an extract, aliquot or sample comprising at
least one of cannabidiol (CBD), .DELTA..sup.9 THC, .DELTA..sup.8
THC, and other cannabinoids from the natural or a synthetic source,
wherein optionally the natural or a synthetic source comprises or
is derived from a plant or a microbial material;
[0231] b. providing a continuous chromatography apparatus
comprising a plurality of stations and a plurality of
chromatography columns, wherein optionally the plurality of
stations and the plurality of chromatography columns comprise at
least 2, 3, 4, 5, 6, 7, or 8 or more, or between about 3 and 30,
stations and/or chromatography columns;
[0232] c. introducing or loading the extract, aliquot or sample
into a first column at a first station, wherein the at least one of
cannabidiol (CBD), .DELTA..sup.9 THC, .DELTA..sup.8 THC, and other
cannabinoids reversibly bind to the first column,
[0233] wherein optionally some of or substantially most of the
extract, aliquot or sample material not reversibly bound to the
first column at the first station is removed with a first wash
solution before the column is moved to a next or a second
station;
[0234] d. moving the first column to a next or a second
station;
[0235] e. eluting the extract, aliquot or sample from the first
chromatography column with a first elution solution at the next or
second station, wherein the first elution solution does not elute
the CBD, .DELTA..sup.9 THC and .DELTA..sup.8 THC, which remains on
the first chromatography column;
[0236] f. collecting the first elution fractions from the first
column;
[0237] g. removing all or substantially most of the first elution
solution from the first elution fractions off the first column to
produce a first purified extract substantially free of CBD,
.DELTA..sup.9 THC, and .DELTA..sup.8 THC;
[0238] h. optionally repeating steps d to g with at least one
additional elution solution off the first column;
[0239] i. when the first column is moved to the next or second
station, a second column is introduced or moved into the first
station where another batch of the extract, aliquot or sample is
introduced or loaded;
[0240] j. moving the second column to the next or second station
when the first column is moved to a third station;
[0241] k. eluting the extract or material reversibly bound on the
first column (which comprises at least one of cannabidiol (CBD),
.DELTA..sup.9 THC, .DELTA..sup.8 THC, and other cannabinoids) from
the first column with a second elution solution at the next or
second station,
[0242] wherein optionally the elution using the second elution
solution is by a gradient elution process and the .DELTA..sup.9 THC
and/or .DELTA..sup.8 THC and/or other cannabinoids are separately
collected in different or separate gradient elution fractions,
[0243] wherein optionally, if the elution is by a gradient elution
process: in a first gradient solvent non-polar compounds and
terpenes are eluted off the column; in a second gradient solvent
cannabidiol (CBD) is eluted off the column; in a third gradient
solvent a CBD/tetrahydrocannabinol (THC) mix is first eluted and
then remaining THC is eluted off the column; in a fourth gradient
remaining cannabinoids are eluted off the column, wherein
optionally the remaining cannabinoids comprise CBC, CBG, CBN; and,
in a fifth gradient solvent all remaining polar compounds are
eluted off the column, wherein the remaining polar compounds
comprises cannabidiolic acid (CBDA) and tetrahydrocannabinolic acid
(THCA);
[0244] l. collecting second elution fractions from the first
chromatography column; m. removing all or substantially most of the
second elution solution from the first elution fractions to produce
a second purified extract comprising at least 75% of the total
amount of CBD, .DELTA..sup.9 THC, and .DELTA..sup.8 THC, or between
about 60% and 90% of CBD, .DELTA..sup.9 THC, and .DELTA..sup.8 THC,
or between about 50% and 95% of CBD, .DELTA..sup.9 THC, and
.DELTA..sup.8 THC;
[0245] n. optionally repeating steps j to m with at least one
second additional elution solution;
[0246] o. optionally repeating steps i to m with at least one
additional column;
[0247] p. mixing the second purified extract with a reaction
mixture comprising an organic solvent and a catalyst;
[0248] q. reacting the mixture at a controlled temperature for a
period of time, wherein optionally the period of time is between
about 30 seconds and 30 minutes;
[0249] r. adding a base to the mixture;
[0250] s. separating the mixture into an aqueous phase and an
organic phase, if an organic solvent is used:
[0251] t. loading the organic phase onto a normal phase
chromatography column;
[0252] u. eluting the organic phase with a second organic solvent;
and
[0253] v. recovering a third purified extract comprising at least
about 30%, 40%, 50% or 60% or more of .DELTA..sup.8-THC and
.DELTA..sup.9-THC.
[0254] In some embodiments, the method further comprises repeating
steps p to v.
[0255] In some embodiments, the base comprises an alkali metal
hydrogen carbonate, an alkali metal carbonate, lithium hydroxide
(LiOH), sodium hydroxide (NaOH), potassium hydroxide (KOH),
rubidium hydroxide (RbOH), cesium hydroxide (CsOH), magnesium
hydroxide (Mg(OH)), calcium hydroxide (Ca(OH)), strontium hydroxide
(Sr(OH)), barium hydroxide (Ba(OH)).
[0256] In some embodiments, the controlled temperature is in a
range of -10.degree. C. to 40.degree. C.
[0257] In some embodiments, the extract comprises at least 25% CBD
by weight, or between about 10% to 40% CBD by weight, or between
about 5% to 50% CBD by weight.
[0258] In some embodiments, at least one of the first elution
solution, the additional elution solution, the second elution
solution, and the second additional elution solution comprises
methanol, ethanol, propanol, butanol, chloroform, dichloromethane,
dichloroethane, acetic acid, formic acid, oxalic acid, glycolic
acid, ammonium hydroxide, water, or combinations thereof.
[0259] In some embodiments, the organic solvent comprises ethylene
dichloride, chloroform, methylene dichloride, toluene, xylene,
benzene, hexane, pentane, or combinations thereof.
[0260] In some embodiments, the catalyst comprises a Lewis acid or
a non-oxidizing acid. In some embodiments, the catalyst comprises
p-toluenesulfonic acid
[0261] In some embodiments, the method further comprises
dissolving, diluting or filtering the extract, aliquot or sample of
the natural or synthetic source in a first solvent before
introducing or loading the extract, aliquot or sample of the
natural or synthetic source into the first chromatography column,
or the second or additional chromatography columns at the first
station.
[0262] In some embodiments, the first solvent comprises methanol,
ethanol, propanol, isobutanol, butanol, isopropanol,
tetrahydrofuran, chloroform, methylene dichloride, dichloroethane,
water, or combinations thereof.
[0263] In some embodiments, the method further comprises filtering
the extract, aliquot or sample of the natural or synthetic source
to remove all or substantially most of the material's solids and
color bodies before introducing or loading the extract, aliquot or
sample into the first column, or before the initial loading of the
extract, aliquot or sample in the second or additional columns at
the first station.
[0264] In some embodiments, at least one of the first
chromatography column, the second chromatography column, or the at
least one additional chromatography column comprises a normal phase
chromatography column, a reverse phase chromatography column, or an
ion exchange chromatography column.
[0265] In some embodiments, the method further comprises measuring
the amount of .DELTA..sup.9-THC in the organic phase.
[0266] In some embodiments, the first and second elution solutions
are different.
[0267] In some embodiments, there are at least 4 columns, at least
four elution solutions, and at least four purified extracts.
[0268] In some embodiments, the at least four elution solutions are
different.
[0269] In alternative embodiments, the natural or synthetic
material, or the extract, aliquot or sample, is filtered before
being loaded on to the column, and optionally the natural or
synthetic material, or the extract, aliquot or sample, is mixed
with silica, diatomaceous earth (DE), bentonite clay (BC), or
equivalents, or mixtures thereof, before filtering, and optionally
the natural or synthetic material, or the extract, aliquot or
sample, is chilled before being mixed with the silica, DE, BC or
equivalents or is chilled before being filtered.
[0270] In alternative embodiments, the microbial material comprises
or is derived from a bacterium, an algal cell, a lichen or a yeast,
or a recombinant bacterium, algal cell, lichen or yeast cell.
[0271] In alternative embodiments, the plant material comprises or
is derived from a botanical drug substance, including a hemp or a
cannabis plant, wherein optionally the cannabis plant is a Cannabis
sativa, a Cannabis chemovar, or variants thereof, or a
decarboxylated Cannabis plant material. In alternative embodiments,
the plant or starting material comprises oils or extracts from a
trichome or a trichome fraction of a pubescent plant, or an algae
or a lichen; and optionally the oils or extracts are from the plant
or microbial material by: washing, contacting or exposing the
trichome or trichome fraction, or the pubescent plant, algae or
lichen, with: at least one non-polar, organic solvent; at least one
polar, organic solvent; or, a mix of at least one non-polar,
organic solvent with (and) at least one polar, organic solvent.
[0272] The details of one or more exemplary embodiments of the
invention are set forth in the accompanying drawings and the
description below. Other features, objects, and advantages of
embodiments as provided herein will be apparent from the
description and drawings, and from the claims.
[0273] All publications, patents, patent applications cited herein
are hereby expressly incorporated by reference for all
purposes.
DESCRIPTION OF DRAWINGS
[0274] The drawings set forth herein are illustrative of exemplary
embodiments provided herein and are not meant to limit the scope of
the invention as encompassed by the claims.
[0275] FIG. 1 schematically illustrates an exemplary method as
provided herein using a continuous chromatography apparatus
comprising a plurality of stations and a plurality of
chromatography columns.
[0276] Like reference symbols in the various drawings indicate like
elements.
DETAILED DESCRIPTION
Chapter I
[0277] In alternative embodiments, provided are continuous
isolation and purification processes for preparing a substantially
pure cannabidiol or a product enriched in cannabidiol from natural
or synthetic sources, including plant or microbial material
extracts. In alternative embodiments, provided herein are improved
methods for converting cannabidiol (CBD) to .DELTA..sup.8-THC and
.DELTA..sup.9-THC, including a purification and conversion process
based on a simple combination of continuous chromato-graphic
gradient elutions and semi continuous isomerization reactions. This
exemplary process is simple, efficient and economic.
[0278] In alternative embodiments, provided are methods of
preparing cannabinoids in substantially pure form starting from
plant extract material and conversion of the purified CBD to form
both .DELTA..sup.8-THC and .DELTA..sup.9-THC and subsequent
purification of the produced .DELTA..sup.8-tetrahydrocannabinol
into .DELTA..sup.9-tetrahydrocannabinol using continuous
chromatography.
[0279] In alternative embodiments, provided are processes for
producing and isolating cannabinoids from cannabis and hemp
extracts which contain cannabinoids in minute amounts. In
alternative embodiments, provided are processes for producing and
isolating cannabinoids from natural materials, including plant or
plant extracts, microbes, or botanical drug substances, or
synthetically and semi-synthetically prepared cannabinoid products,
or from recombinantly engineered microbes, e.g., yeasts or bacteria
recombinantly engineered to express one or more cannabinoids. In
one embodiment, exemplary methods are inexpensive and provide
specific cannabinoid concentrates (e.g., of CBD, .DELTA..sup.8-THC,
.DELTA..sup.9-THC) of high purity.
[0280] In one embodiment, exemplary methods provide a simple and
economical continuous process for separating and concentrating
cannabinoids from solvent-extracted cannabinoid containing
materials. In one embodiment, exemplary methods provide a method
that first converts the substantially isolated CBD into a mixture
of .DELTA..sup.8-THC and .DELTA..sup.9-THC, and then subsequently
purifies and/or isolates the .DELTA..sup.8-THC and
.DELTA..sup.9-THC. In alternative embodiments, the
solvent-extracted cannabinoid containing materials are derived from
synthetic or biological materials such as hemp and Cannabis or
botanical drug substances, or from microbial materials; and the
solvent extraction methods can be polar solvent extractions,
nonpolar solvent extractions, or the solvent extraction methods can
comprise use of super critical carbon dioxide or mixtures thereof.
The solvent extraction methods can extract cannabinoids
substantially from the synthetic or biological, e.g., plant,
matter, along with other plant matter comprising lipids, waxes,
monoterpenes, sesquiterpenes, hydrocarbons, alkaloids, flavonoids
and chlorophylls.
[0281] In alternative embodiments, methods provided herein comprise
subjecting cannabinoid containing solvent extract starting
materials to a number of chromatographic resins in various
contacting steps using various gradient elution solutions.
[0282] In alternative embodiments, the cannabinoids which can be
fractionated and isolated using methods as provided herein, or
which can be produced in reactions as provided herein, or from
which the solvent extracts are derived can be from, or can
comprise: .DELTA..sup.8tetrahydrocannabinol (.DELTA..sup.8 THC);
.DELTA..sup.9tetrahydrocannabinol (.DELTA..sup.9 THC); cannabidiol
(CBD); cannabinol (CBN); cannabigerol (CBG); cannabichromene (CBC);
cannabidivarol (CBDV); tetrahydrocannabidiol (THCBD);
tetrahydrocannabigerol (THCBG); tetrahydrocannabichromene (THCBC);
tetrahydrocannabidivarol (THCBDV), or combinations thereof,
including carboxylic acid precursors of the foregoing compounds and
related naturally occurring compounds and their derivatives.
[0283] In alternative embodiments, provides are methods of
preparing or obtaining a substantially pure cannabinoid or a
product enriched in a given cannabinoid comprising: [0284] (i)
obtaining an extract or extract solution containing a cannabinoid
or a cannabinoid acid from a synthetic or biological, e.g., plant,
material; [0285] (ii) optionally filtering the extract of step (i)
to remove all or substantially all solids and color bodies; [0286]
(iii) removing the extract solvent (as an extract fraction); [0287]
(iv) continuously loading of an amount of extract solution over a
defined time increment over multiple stationary phase resins
columns such as a normal phase, reverse phase and/or ion exchange
chromatographic resin, e.g., at first station; [0288] (v)
continuously eluting the extract solution using multiple defined
gradient elution solutions at specific time increments and volumes,
e.g., at a second station; [0289] (vi) continuously collecting the
gradient elution fractions; [0290] (vii) removal of the gradient
elution solution from the produced fractions to generate a
substantially purified extract; [0291] (viii) optionally loading of
specific first gradient elution fractions on a reverse phase, ion
exchange or normal chromatographic resin; [0292] (ix) continuously
eluting the first gradient elution fractions with a second gradient
elution solvent; and, [0293] (x) removing of the second gradient
elution solvent from the produced fractions to produce a purified
extract.
[0294] In alternative embodiments, the methods further
comprise:
[0295] a step (xi): loading of first and second gradient elution
solvents onto an ion exchange chromatographic resin;
[0296] a step (xii): eluting an extract solution from the ion
exchange chromatographic resin of step (x) using a gradient solvent
and collecting the gradient elution fractions; and
[0297] (xiii) removing the gradient elution solvent from the
produced fractions of step (xii) to produce purified and
substantially purified extract.
[0298] In alternative embodiments, provided are methods of
substantially converting CBD to .DELTA..sup.8-THC and
.DELTA..sup.9-THC comprising: [0299] (a) providing a reaction
mixture comprising a catalyst in an organic solvent; [0300] (b)
adding CBD material, e.g., a CBD purified or isolated using steps
(vi) and steps (xiii), above; [0301] (c) mixing said reaction
mixture; [0302] (d) reacting mixture for a period of time at a
controlled temperature; [0303] (e) adding a base to the reaction
mixture; [0304] (f) allowing the mixture to separate into an
aqueous phase and an organic phase; [0305] (g) removing the organic
phase; and, [0306] (h) loading the organic phase onto a normal
phase chromatography column [0307] (i) eluting the organic phase
with an organic solvent and recovering substantially pure CBD,
.DELTA..sup.8 THC and .DELTA..sup.9 THC [0308] optionally,
repeating steps (a) through (i).
[0309] In alternative embodiments, a "substantially pure"
preparation of cannabinoid is defined as a preparation having a
chromatographic purity (of the desired cannabinoid or cannabinoid
acid) of greater than about 75%, or greater than about 96%, or
greater than about 97%, or greater than about 98%, or greater than
about 99%, or greater than about 99.5%, or between about 70% and
99.9%, as determined by area normalization of an HPLC profile.
[0310] In alternative embodiments, the term "product enriched in a
given cannabinoid" encompasses preparations having at least about
50%, or greater than about 75%, or greater than about 90%, 95% or
98%, or between about 50% and 99.9%, chromatographic purity, for
the desired cannabinoid.
[0311] In alternative embodiments, the term "about" is within 20%
of the stated value, or 19%, or 18%, or 17%, or 16%, or 15%, or
14%, or 13%, or 12%, or 11%, or 10%, or 9%, or 8%, or 7%, or 6%, or
5%, or 4%, or 3%, or 2%, or 1%, or 0.5%, or 0.1%, or 0.05%, or
0.01%, or is between 20% and 0.01% of the stated value.
[0312] In alternative embodiments, a non-purified, or
non-substantially purified, product can comprise a greater
proportion of impurities, non-target materials and/or other
cannabinoids than a "substantially pure" preparation. The
cannabinoid can be (e.g., a cannabinoid purified or isolated by, or
made by a reaction of, a method as provided herein can be):
.DELTA..sup.8-tetrahydrocannabinol (.DELTA..sup.8-THC);
.DELTA..sup.9-tetrahydrocannabinol (.DELTA..sup.9-THC); cannabidiol
(CBD); cannabinol (CBN); cannabigerol (CBG); cannabichromene (CBC);
cannabidivarol (CBDV); tetra-hydrocannabidiol (THCBD);
tetra-hydrocannabigerol (THCBG); tetra-hydrocannabichromene
(THCBC); or, tetra-hydrocannabidivarol (THCBDV); a carboxylic acid
precursor of the foregoing compounds; and related naturally
occurring compounds and their derivatives.
[0313] In alternative embodiments, the term "cannabinoids", e.g., a
cannabinoid purified or isolated by, or made by a reaction of a
process as provided herein, includes or refers to a family of
natural products that can contain a 1,1'-di-methyl-pyrane ring, a
variedly derivatized aromatic ring and/or a variedly unsaturated
cyclohexyl ring and their immediate chemical precursors.
[0314] In alternative embodiments, the term "cannflavins", e.g., a
cannflavin purified or isolated by, or made by a reaction of a
process as provided herein, includes or refers to a family of
natural products that can contain a 1,4-pyrone ring fused to a
variedly derivatized aromatic ring and linked to a second variedly
derivatized aromatic ring.
[0315] In alternative embodiments, the term "Lewis acid" refers to
a powerful electron pair acceptor; and examples include but are by
no means limited to BF3Et2O (boron trifluoride diethyl etherate),
p-toluenesulfonic acid and boron trifluoride. In alternative
embodiments, the term "non-oxidizing acid" refers to hydrobromic,
hydrochloric, hydrofluoric, acetic, benzoic, chloroacetic, formic,
phosphoric, sulfuric, trifluroacetic and oxalic acids.
[0316] In alternative embodiments, the term "essential oils", e.g.,
an essential oil used as a starting material in a process as
provided herein, or an essential oil that may be isolated by a
process as provided herein, includes or refers to a family of
natural products that can contain a multiple of the 5-membered
isoprene unit variedly substituted, often cyclized to form one or
more ring systems; they can also contain series of aldehydes and/or
ketones and esters of a variety of carboxylic acid substituted
compounds.
[0317] In alternative embodiments, provided are methods for
extracting and/or purifying cannabinoids from any natural or
synthetic sources, including plant or microbial material or
extracts known to contain such cannabinoids, cannflavins and
essential oils; and, optionally to purify cannflavins and to
optionally purify essential oils. In alternative embodiments, the
extract is passed through a series of chromatographic columns, for
example, a normal phase column, a reversed phase column or an ion
exchange column as a continuous simulated moving bed
configuration.
[0318] In one embodiment, the chromatographic column is arranged
for gradient elution fractioning using normal phase, reverse phase
and/or ion exchange chromatography. In one embodiment
.DELTA..sup.9-THC and CBD are fractionated out of the eluent. For
example, in one embodiment, as the extract is passed over the
column, .DELTA..sup.9-THC and CBD are differentially retained or
detained (e.g., reversibly bound) on the column. As a result, as
the extract comes off the column after gradient elution transition,
the initial fractions eluted off the column will be (substantially)
free of .DELTA..sup.9-THC and CBD. The fractions free of
.DELTA..sup.9-THC and CBD are pooled, thereby producing an extract
with .DELTA..sup.9-THC and CBD substantially removed (e.g., in
alternative embodiments, "substantially removed" or "substantially
fractionated" means at least 85%, 90%, 95%, 98%, 99% or 99.5% or
more removed or fractionated).
[0319] In some embodiments, (substantially) only .DELTA..sup.9-THC
is substantially fractionated out of the eluent. For example, in
one embodiment, as the extract or pooled fractions from a previous
normal phase, reverse phase and/or ion exchange elution are passed
over the column, .DELTA..sup.9-THC is differentially retained or
detained (e.g., reversibly bound) on the column. As the extracts
pooled from previous elution fractions come off the column, the
initial fractions eluted off the column will be free of
.DELTA..sup.9-THC. These fractions free of .DELTA..sup.9-THC are
pooled, thereby producing an extract with .DELTA..sup.9-THC
substantially removed (e.g., in alternative embodiments,
"substantially removed" or "substantially fractionated" means at
least 85%, 90%, 95%, 98%, 99% or 99.5% or more removed or
fractionated).
[0320] In some embodiments, when using a continuous chromatography
apparatus or device, a series of columns are arranged, for example,
3, 4, 5, 6, 7, 8, 9, or 10 or more, or between 3 and 30, columns
are arranged in a continuous rotation traveling through a series of
contact points where gradient elution solutions and extract
solution are introduced at fixed points for a period of time
allowing for continuous loading and elution, and collection of
fractions. The first column is loaded with extract solution at the
first position (or station). The first column is then moved to the
second position where the first gradient elution is introduced (or
loaded) while at the same time the second column is loaded with
extract solution at position one. The first column then rotates
(i.e., is moved) to the third position where the second gradient
solvent is introduced, the second column moves to the second
position where the first gradient solvent is introduced and the
third column is loaded with extract solution at position one. The
first column then moves to the fourth position where the third
gradient solvent is introduced, the second column moves to the
third position where the second gradient solvent is introduced, the
third column moves to the second position where the first gradient
solvent is introduced and the fourth column is loaded with extract
solution at position one. The first column then moves to the fifth
position where the fourth gradient solvent is introduced, the
second column moves to the fourth position where the third gradient
solvent is introduced, the third column moves to the third position
where the second gradient solvent is introduced, the fourth column
moves to the second position where the first gradient solvent is
introduced and the fifth column is loaded with extract solution at
position one. The first column then moves to the sixth position
where the fifth gradient solvent is introduced, the second column
moves to the fifth position where the fourth gradient solvent is
introduced, the third column moves to the fourth position where the
third gradient solvent is introduced, the fourth column moves to
the third position where the second gradient solvent is introduced,
the fifth column moves to the second position where the first
gradient solvent is introduced and the sixth column is loaded with
extract solution at position one. The first column then moves or
returns to the first position where extract solution is loaded, the
second column moves to the sixth position where the fifth gradient
solvent is introduced, the third column moves to the fifth position
where the fourth gradient solvent is introduced, the fourth column
moves to the fourth position where the third gradient solvent is
introduced, the fifth column moves to the third position where the
second gradient solvent is introduced and the sixth column moves to
the second position where the first gradient solvent is introduced.
In a particular embodiment, the first gradient solvent elutes CBD,
CBG and CBN and is substantially fractionated in the eluent.
[0321] For example, in one embodiment, as the extract or pooled
fractions from a normal phase, reverse phase and/or ion exchange
elution are passed over the column, CBD, CBG and CBN is
differentially produced in the eluent (e.g., by use of a gradient
elution process). In a particular embodiment, second gradient
solvent elutes CBD, which is substantially fractionated in the
eluent. For example, in one embodiment, as the extract or pooled
fractions from a normal phase, reverse phase and/or ion exchange
elution are passed over the column, CBD is differentially produced
in the eluent. In a particular embodiment, the third gradient
solvent elutes CBD and .DELTA..sup.9-THC and is substantially
fractionated in the eluent. For example, in one embodiment, as the
extract or pooled fractions from a normal phase, reverse phase
and/or ion exchange elution are passed over the column, CBD and
.DELTA..sup.9-THC is differentially produced in the eluent. In a
particular embodiment the fourth gradient elutes .DELTA..sup.9-THC
and is substantially fractionated in the eluent. For example, in
one embodiment, as the extract or pooled fractions from a normal
phase, reverse phase and/or ion exchange elution are passed over
the column, .DELTA..sup.9-THC is differentially produced in the
eluent. In a particular embodiment the fifth gradient elutes CBC,
THC-A, terpenes and .DELTA..sup.9-THC and is substantially
fractionated in the eluent. For example, in one embodiment, as the
extract or pooled fractions from a normal phase, reverse phase
and/or ion exchange elution are passed over the column, CBC, THC-A,
terpenes and .DELTA..sup.9-THC is differentially produced in the
eluent.
[0322] In some embodiments, when using a continuous chromatography
apparatus or device, a series of columns are arranged, for example,
3, 4, 5, 6, 7, 8, 9, or 10 or more, or between 3 and 30, columns
are arranged in a continuous rotation traveling through a series of
contact points where gradient elution solvents and extract solution
are introduced at fixed points for a period of time allowing for
continuous loading and elution, and collection of fractions. The
first column is loaded with extract solution at the first position
(or station). The first column is then moved to the second position
where the first gradient elution solvent is introduced (or loaded)
while at the same time the second column is loaded with extract
solution at position one. The first column then rotates (i.e., is
moved) to the third position where the second gradient solvent is
introduced, the second column moves to the second position where
the first gradient solvent is introduced, and the third column is
loaded with extract solution at position one. The first column then
moves to the fourth position where the third gradient solvent is
introduced, the second column moves to the third position where the
second gradient solvent is introduced, the third column moves to
the second position where the first gradient solvent is introduced,
and the fourth column is loaded with extract solution at position
one. The first column then moves to the fifth position where the
fourth gradient solvent is introduced, the second column moves to
the fourth position where the third gradient solvent is introduced,
the third column moves to the third position where the second
gradient solvent is introduced, the fourth column moves to the
second position where the first gradient solvent is introduced and
the fifth column is loaded with extract solution at position one.
The first column then moves to the sixth position where the fifth
gradient solvent is introduced, the second column moves to the
fifth position where the fourth gradient is introduced, the third
column moves to the fourth position where the third gradient
solvent is introduced, the fourth column moves to the third
position where the second gradient solvent is introduced, the fifth
column moves to the second position where the first gradient
solvent is introduced and the sixth column is loaded with extract
solution at position one. The first column then moves or returns to
the first position where extract solution is loaded, the second
column moves to the sixth position where the fifth gradient solvent
is introduced, the third column moves to the fifth position where
the fourth gradient solvent is introduced, the fourth column moves
to the fourth position where the third gradient solvent is
introduced, the fifth column moves to the third position where the
second gradient solvent is introduced and the sixth column moves to
the second position where the first gradient solvent is introduced.
In a particular embodiment the first gradient solvent elutes CBD,
CBG and CBN and is substantially fractionated in the eluent. For
example, in one embodiment, as the extract or pooled fractions from
a normal phase, reverse phase and/or ion exchange elution are
passed over the column, CBD, CBG and CBN is differentially produced
in the eluent (e.g., by use of a gradient elution process). In a
particular embodiment second gradient solvent elutes CBD and is
substantially fractionated in the eluent. For example, in one
embodiment, as the extract or pooled fractions from a normal phase,
reverse phase and/or ion exchange elution are passed over the
column, CBD is differentially produced in the eluent. In a
particular embodiment the third gradient solvent elutes CBD and
.DELTA..sup.9-THC and is substantially fractionated in the eluent.
For example, in one embodiment, as the extract or pooled fractions
from a normal phase, reverse phase and/or ion exchange elution are
passed over the column, CBD and .DELTA..sup.9-THC is differentially
produced in the eluent. In a particular embodiment, the fourth
gradient elutes .DELTA..sup.9-THC and is substantially fractionated
in the eluent. For example, in one embodiment, as the extract or
pooled fractions from a normal phase, reverse phase and/or ion
exchange elution are passed over the column, .DELTA..sup.9-THC is
differentially produced in the eluent. In a particular embodiment
the fifth gradient elutes CBC, THC-A, terpenes and
.DELTA..sup.9-THC and is substantially fractionated in the eluent.
For example, in one embodiment, as the extract or pooled fractions
from a normal phase, reverse phase and/or ion exchange elution are
passed over the column, CBC, THC-A, terpenes and .DELTA..sup.9-THC
is differentially produced in the eluent. The second gradient
solvent n containing CBD is combined with a Lewis acid or
non-oxidizing acid catalyst, optionally additional CBD or
substantially pure CBD and/or .DELTA.8-tetrahydrocannabinol
(.DELTA.8-THC) and/or .DELTA.9-tetrahydrocannabinol (.DELTA.9-THC)
can be added to the reaction mixture, mixing said reaction mixture
for a period of time; adding a neutralizing agent to said mixture;
filtration of catalyst and neutralizing agent from mixture;
optionally allowing mixture to separate into an aqueous and organic
phase; optionally adding organic phase to a chromatography column
and eluting the (.DELTA.8-THC) and/or .DELTA.9-tetrahydrocannabinol
(.DELTA.9-THC) from the organic phase. The tetrahydrocannabinol can
then be combined with suitable excipients known in the art, thereby
forming a pharmaceutical composition. In one embodiment, the
mixture is allowed to separate into an aqueous phase and an organic
phase; and optionally the process further comprises removing the
organic phase.
[0323] In some embodiments, (substantially) only CBD is
substantially fractionated out of the eluent. That is, as the
extract or pooled fractions from a previous normal phase, reverse
phase and/or ion exchange elution are passed over the column, CBD
is differentially retained or detained (e.g., reversibly bound) on
the column. As a result, as the extract or pooled previous elution
fractions comes off the column, the initial fractions eluted off
the column will be (substantially) free of CBD. These fractions
free of CBD are pooled, thereby producing an extract with CBD
substantially removed (e.g., in alternative embodiments,
"substantially removed" or "substantially fractionated" means at
least 85%, 90%, 95%, 98%, 99% or 99.5% or more removed or
fractionated).
[0324] In some embodiments, .DELTA..sup.9-THC carboxylic acid
species or .DELTA..sup.9-tetra-hydrocannabinolic acid (THCA) and
the CBD carboxylic acid species cannabidiolic acid (CBDA) are
fractionated out of out of the eluent. For example, in one
embodiment, as the extract or pooled fractions from a first elution
are passed over the column, .DELTA..sup.9-THCA and CBDA are
differentially retained or detained (e.g., reversibly bound) on the
column. As a result, as the extract or pooled first elution
fractions comes off the column, the initial fractions eluted off
the column will be (substantially) free of .DELTA..sup.9-THCA and
CBDA. These fractions (substantially) free of .DELTA..sup.9-THCA
and CBDA are pooled, thereby producing an extract with
.DELTA..sup.9-THCA and CBDA substantially removed (e.g., in
alternative embodiments, "substantially removed" or "substantially
fractionated" means at least 85%, 90%, 95%, 98%, 99% or 99.5% or
more removed or fractionated).
[0325] In some embodiments, the .DELTA..sup.9-THC can be eluted
from the column, extracted or concentrated, for purifying, or
substantially purifying, .DELTA..sup.9-THC. In alternative
embodiments, the chromatographic column is arranged for
fractionating (e.g., sequentially fractionating) a specific
cannabinoid or groups of cannabinoids or their carboxylic acid
species, cannflavin or essential oil or class of cannabinoids,
cannflavins or essential oils out of the eluent, for example,
cannabidiol (CBD), cannabinol (CBN), cannabigerol (CBG),
cannabichromene (CBC), cannabidivarol (CBDV), tetrahydrocannabidiol
(THCBD), tetrahydrocannabigerol (THCBG), tetrahydrocannabichromene
(THCBC), tetrahydrocannabidivarol (THCBDV), .DELTA..sup.8-THC, the
carboxylic acid precursors of the foregoing compounds, and related
naturally occurring compounds and their derivatives. In alternate
embodiments, the chromatographic column is arranged for
fractionating (e.g., sequentially fractionating) cannflavins and
related naturally occurring compounds and their derivatives. In
alternate embodiments, the system is arranged to fractionate the
components of essential oils. The list of compounds provided herein
is not exhaustive and is in no way intended to be limiting. In
these embodiments, the compound(s) of interest are retained or
detained (e.g., reversibly bound) on the column so that fractions
(alternatively, the last fractions) of the extract eluted from the
column contain the compounds(s) of interest. In alternative
embodiments, fractions containing the compound(s) of interest are
pooled. In some embodiments, different compounds can be extracted
with different solvents and then combined into a single extract. As
will be appreciated by one knowledgeable in the art, in this
manner, several different cannabinoids could be purified from a
single extract.
[0326] In alternative embodiments, the "plant material" or "plant
extracts" comprise or are derived from one or more cannabis or hemp
plants, or from other plants. In alternative embodiments, the
microbial material or extracts comprise or are derived from yeast,
lichen, algae or bacteria. The term "plant material" encompasses a
plant or plant part (e.g. bark, wood, leaves, stems, roots,
flowers, fruits, seeds, berries or parts thereof) as well as
exudates, and includes material falling within the definition of
"botanical raw material" in the Guidance for Industry Botanical
Drug Products Draft Guidance, August 2000, US Department of Health
and Human Services, Food and Drug Administration Centre for Drug
Evaluation and Research. The term "cannabis plant(s)" encompasses
wild type Cannabis sativa and also variants thereof, including
Cannabis chemovars (varieties characterized by virtue of chemical
composition) which naturally contain different amounts of the
individual cannabinoids, also Cannabis sativa subspecies indica
including the variants var. indica and var. kafiristanica, Cannabis
indica and also plants which are the result of genetic crosses,
self-crosses or hybrids thereof. The term "Cannabis plant material"
also can encompass plant material derived from one or more cannabis
plants, and can comprise any "cannabis plant material" including,
e.g., herbal Cannabis and dried Cannabis biomass. The term
"Cannabis plant material" also can encompass "decarboxylated
Cannabis plant material", which refers to Cannabis plant material
which has been subject to a decarboxylation step in order to
convert cannabinoid acids to the corresponding free cannabinoids.
In alternative embodiments, a starting material for a purification
process as provided herein is an extract solution containing a
cannabinoid or cannabinoid acid obtained from a natural or a
synthetic source, e.g., a plant or microbial material. In
alternative embodiments, the "extract solution containing a
cannabinoid or cannabinoid acid" comprises a solvent extract of a
plant or microbial material. Solvents used for extraction for use
in the preparation of extract solutions can comprise non-polar
solvents, polar solvents such as ethanol, methanol or water, or
organic solvents such as liquid carbon dioxide, and combinations
thereof. The solvent can be an organic solvent, selected from the
group consisting of: non-polar solvents include liquid non-polar
solvents comprising lower C1-C12, preferably C3 to C8, straight
chain or branched chain alkanes for example, methane, ethane,
propane, butane, pentane, toluene, trimethylpentane; a low
molecular weight alcohol, polar solvents consisting of for example,
ethanol, methanol, water; a low molecular weight chlorinated
hydrocarbon, for example, chloroform and dichloromethane; or a
supercritical fluid such as CO.sub.2 with or without an organic
solvent modifier.
[0327] In alternative embodiments, the extract is prepared by
dissolving or partially dissolving the natural or synthetic, or the
plant or microbial material in a solvent, removing insoluble
material from the resultant solution (optionally by filtration with
or without activated carbon, precipitation, centrifugation and the
like), and optionally removing some or all of the extraction
solvent from the solution (optionally by rotary evaporation) to
form an extract or extract solution or concentrate containing a
cannabinoid or cannabinoid acid.
[0328] In alternative embodiments, the plant or microbial material
or extracts used as a starting material comprise oils or extracts
from a trichome or a trichome fraction of a pubescent plant, or an
algae or a lichen. The oils or extracts can be "harvested" from the
plant or microbial material by: washing, contacting or exposing the
trichome or trichome fraction, or the pubescent plant, algae or
lichen, with: at least one non-polar, organic solvent; at least one
polar, organic solvent; or, a mix of at least one non-polar,
organic solvent with (and) at least one polar, organic solvent, and
collecting or separating the solvent from the trichome or trichome
fraction, or the pubescent plant, algae or lichen, wherein the
solvent comprises the oil or extract to be further processed using
a method as provided herein. In alternative embodiments, the
pubescent plant or plant part, the algae or algae part, or the
lichen or lichen part, other than harvesting or collecting, is
unprocessed or has not been physically processed, or has not been
exposed to: a solvent or aqueous solution; a heating or drying
process; a dehydration process; a crushing process; and/or, a
chopping, macerating or mincing process, since (after) its harvest,
before the trichome or trichome fraction, or the pubescent plant,
algae or lichen is washed with, contacted by or exposed to: at
least one non-polar, organic solvent; at least one polar, organic
solvent; or, a mix of at least one non-polar, organic solvent with
(and) at least one polar, organic solvent.
[0329] In alternative embodiments, extractions comprise using a
technique referred to as accelerated solvent extraction, which can
use subcritical water or any combination of water and solvent. In
one embodiment, when isolating cannabinoid acids, a modified pH
gradient elution solvent is used. The primary purpose of this pH
adjustment (the modified pH gradient) is to promote or prevent
ionization of the cannabinoid acid. pH modified gradient elution
solvents s may be achieved by the additional of a small volume of
acid or base to the solvent. It may be sufficient to add a
relatively weak acid, such as acetic acid, oxalic acid, glycolic
acid, carbonic acid or ammonium hydroxide or a small amount of base
or buffering agent such as sodium hydroxide, magnesium hydroxide,
sodium carbonate or sodium bicarbonate. For any given purification
process the optimal amount and type of acid or base used may be
determined empirically. An alternative exemplary acidified solvent
is 0.1% acetic acid in ethanol or 0.1% sodium hydroxide in ethanol.
In alternative embodiments, the neutralizing agent consists of for
example sodium hydroxide, sodium carbonate, potassium carbonate,
and potassium t-amylate, sodium bicarbonate.
[0330] Acidified non-polar and polar solvents of the types
described above can be useful in preparation of gradient elutions
using ion exchange chromatography. The solvents used in the
conversion can comprise an organic solvent, e.g., a non-polar
solvent, including a liquid non-polar solvent comprising lower
C1-C12, or C3 to C8, straight chain or branched chain alkanes for
example, methane, ethane, propane, butane, pentane, toluene,
trimethylpentane, hexane; a low molecular weight alcohol, polar
solvents consisting of for example, ethanol; a low molecular weight
chlorinated hydrocarbon, for example, chloroform and
dichloromethane; a heterocyclic compound or cyclic ether for
example, tetrahydrofuran and 2-methyltetrahydrofuran and aromatic
ring hydrocarbons such as benzene, toluene, xylene and
ethylbenzene.
[0331] In alternative embodiments, the plant material is subjected
to a decarboxylation step prior to solvent extraction. The purpose
of the decarboxylation step is to convert cannabinoid acids present
in the plant or microbial material to the corresponding free
cannabinoids. In alternative embodiments, the decarboxylation is
carried out by heating the plant or microbial material to a defined
temperature for a suitable length of time. Decarboxylation of
cannabinoid acids is a function of time and temperature, thus at
higher temperatures a shorter period of time will be taken for
complete decarboxylation of a given amount of cannabinoid acid. In
alternative embodiments selecting appropriate conditions for
decarboxylation consideration includes minimizing thermal
degradation of the desirable, pharmacological cannabinoids into
undesirable degradation products, particularly thermal degradation
of .DELTA..sup.9-THC. In alternative embodiments, the
decarboxylation is carried out in a multi-step heating process in
which the plant or microbial material is: i) heated to a first
temperature for a first (relatively short) time period to evaporate
off retained water and allow for uniform heating of the plant or
microbial material; and ii) the temperature is increased to a
second temperature for a second time period (typically longer than
the first time period) until at least 95% conversion of the acid
cannabinoids to their neutral form has occurred.
[0332] In alternative embodiments, the "extract containing a
cannabinoid or a cannabinoid acid" prepared from the starting plant
or microbial material comprises a "botanical drug substance"
prepared from the plant or microbial material, or a polar or
non-polar solvent solution of such a botantical drug substance. In
the context of this application a "botanical drug substance" is an
extract derived from plant or microbial material, which extract
fulfills the definition of "botanical drug substance" provided in
the Guidance for Industry Botanical Drug Products Draft Guidance,
August 2000, US Department of Health and Human Services, Food and
Drug Administration Centre for Drug Evaluation and Research of: "A
drug substance derived from one or more plants, algae, or
macroscopic fungi. It is prepared from botanical raw materials by
one or more of the following processes: pulverization, decoction,
expression, aqueous extraction, ethanolic extraction, or other
similar processes." "Botanical drug substances" derived from
cannabis plants include primary extracts prepared by such processes
as, for example, maceration, percolation, and solvent
extraction.
[0333] In alternative embodiments, solvent extraction may be
carried out using essentially any solvent that dissolves, or
substantially dissolves, cannabinoids/cannabinoid acids, such as
for example C1 to C5 alcohols (e.g. ethanol, methanol), C5-C12
alkanes (e.g. hexane), norflurane (HFA134a),
1,1,1,2,3,3,3-Heptafluoropropane (or HFA227), chloroform,
dichloromethane, dichloroethane and carbon dioxide. When solvents
such as those listed above are used, the resultant extract
typically contains non-specific lipid-soluble material. This can
optionally be removed by a variety of processes including
filtration to remove solids, "winterization", which involves for
example chilling to -20.degree. C. or lower followed by filtration
to remove waxy ballast, extraction with liquid carbon dioxide and
by distillation.
[0334] In alternative embodiments, any protocol for the preparation
of botanical drug substances from cannabis and hemp plant material
can be used, e.g., as described in International patent application
WO 02/064109. In alternative embodiments, the botanical drug
substance is obtained by carbon dioxide (CO.sub.2) extraction,
polar solvent extraction or non-polar solvent extraction or
combinations thereof followed by a filtration. Optionally a
secondary extraction is performed to remove a substantial
proportion of non-cannabinoid materials, e.g. waxes, wax esters and
glycerides, unsaturated fatty acid residues, terpenes, carotenes,
and flavonoids and other ballast.
[0335] In alternative embodiments, if it is intended to prepare
free cannabinoids from the cannabis plant or microbial material,
then the material is heated to a defined temperature for a defined
period of time in order to partially or substantially decarboxylate
cannabinoid acids to free cannabinoids prior to extraction of the
botanical drug substance. In alternative embodiments, the botanical
drug substance is prepared according to a process comprising the
following steps: i) optional decarboxylation of the plant or
microbial material, ii) extraction with polar or non-polar solvent,
to produce a crude botanical drug substance, iii) optional
precipitation with C1-C5 alcohol to reduce the proportion of
non-target materials, iv) removal of the precipitate (preferably by
filtration), v) optional treatment with activated charcoal, and vi)
evaporation to remove C1-C5 alcohol and water, thereby producing a
final botanical drug substance.
[0336] In alternative embodiments, provided are methods for
converting substantially purified cannabidiol (CBD) to
.DELTA.8-tetrahydrocannabinol (.DELTA.8-THC) and
.DELTA.9-tetrahydrocannabinol (.DELTA.9-THC). As will be
appreciated by one knowledgeable in the art and as discussed below,
the reaction times may be varied somewhat, producing product at
different yields and purities. Furthermore, functional equivalents
may be substituted where appropriate.
[0337] In alternative embodiments, an exemplary method of
converting CBD to .DELTA.8-tetrahydrocannabinol (.DELTA.8-THC) and
.DELTA.9-tetrahydrocannabinol (.DELTA.9-THC) comprises: providing a
reaction mixture comprising a Lewis acid or non-oxidizing acid
catalyst in a reaction solvent, adding a substantially pure CBD or
substantially pure CBD and/or (.DELTA.8-THC) and/or
.DELTA.9-tetrahydrocannabinol (.DELTA.9-THC) to the reaction
mixture, mixing said reaction mixture for a period of time; adding
a neutralizing agent to said mixture; filtration of catalyst and
neutralizing agent from mixture; optionally allowing mixture to
separate into an aqueous and organic phase; optionally removing the
reaction solvent; optionally dissolving organic phase in a second
solvent; adding organic phase to a chromatography column and
eluting the tetrahydrocannabinol from the organic phase. The
tetrahydrocannabinol may then be combined with suitable excipients
known in the art, thereby forming a pharmaceutical composition. In
one embodiment, the mixture is allowed to separate into an aqueous
phase and an organic phase; and optionally the process further
comprises removing the organic phase.
[0338] In alternative embodiments, the tetrahydrocannabinol at
therapeutically effective concentrations or dosages is combined
with a pharmaceutically or pharmacologically acceptable carrier,
excipient or diluent, either biodegradable or non-biodegradable.
Exemplary examples of carriers include, but are by no means limited
to, for example, poly(ethylene-vinyl acetate), copolymers of lactic
acid and glycolic acid, poly(lactic acid), gelatin, collagen
matrices, polysaccharides, poly(D,L lactide), poly(malic acid),
poly(caprolactone), celluloses, albumin, starch, casein, dextran,
polyesters, ethanol, methacrylate, polyurethane, polyethylene,
vinyl polymers, glycols, mixtures thereof and the like. Standard
excipients that can be used include gelatin, casein, lecithin, gum
acacia, cholesterol, tragacanth, stearic acid, benzalkonium
chloride, calcium stearate, glyceryl monostearate, cetostearyl
alcohol, cetomacrogol emulsifying wax, sorbitan esters,
polyoxyethylene alkyl ethers, polyoxyethylene castor oil
derivatives, polyoxyethylene sorbitan fatty acid esters,
polyethylene glycols, polyoxyethylene stearates, colloidol silicon
dioxide, phosphates, sodium dodecyl sulfate, carboxymethylcellulose
calcium, carboxymethylcellulose sodium, methylcellulose,
hydroxyethylcellulose, hydroxypropylcellulose,
hydroxypropylmethycellulose phthalate, non-crystalline cellulose,
magnesium aluminum silicate, triethanolamine, polyvinyl alcohol,
polyvinylpyrrolidone, sugars and starches. See, for example,
Remington: The Science and Practice of Pharmacy, 1995, Gennaro
ed.
[0339] In some embodiments, the catalyst is a Lewis acid, for
example, p-toluenesulfonic acid, boron trifluoride or
BF.sub.3Et.sub.2O. In some embodiments, the BF.sub.3Et.sub.2O
(boron trifluoride diethyl etherate) is in dry methylene chloride,
ethyl acetate, ethanol, hexane or other organic solvent. In yet
other examples, the catalyst may be hydrochloric acid in ethanol or
sulfuric acid in cyclohexane.
[0340] In some embodiments, the catalyst is a non-oxidizing acid,
for example, formic acid, acetic acid or hydrobromic acid. In some
embodiments, the non-oxidizing acid is in dry methylene chloride,
ethyl acetate, ethanol, hexane or other organic solvent.
[0341] In some embodiments, a base is added to the reaction mixture
prior to optionally allowing the reaction mixture to separate into
organic and aqueous phases.
[0342] The base may be an alkali metal hydrogen carbonate,
carbonate of an alkali metal, lithium hydroxide (LiOH), sodium
hydroxide (NaOH), potassium hydroxide (KOH), rubidium hydroxide
(RbOH), cesium hydroxide (CsOH), magnesium hydroxide (Mg(OH)),
calcium hydroxide (Ca(OH)), strontium hydroxide (Sr(OH)), barium
hydroxide (Ba(OH)).
[0343] In some embodiments, the organic layer is dried prior to
eluting. In these embodiments, a suitable drying or dehydration
agent, for example, MgSO.sub.4 or Na.sub.2SO4 is used.
[0344] In yet other embodiments, the process may be carried out
under an inert atmosphere such as a nitrogen (e.g., N.sub.2)
atmosphere.
[0345] In alternative embodiments, and as discussed below, yield is
determined by looking at the peak area for the isolated compound in
the gas chromatography-mass spectra analysis of the crude reaction
product mixture and the final reaction product mixture. It is
important to note that in the prior art, yield is often calculated
on the basis of first isolated crude product before final
purification. In some embodiments of processes provided herein
yield of .DELTA.8-THC and .DELTA.9-THC is at least about 75%; in
other embodiments, the yield of .DELTA.8-THC and .DELTA.9-THC is at
least about 90%; and in other embodiments, yield of .DELTA.8-THC
and .DELTA.9-THC is at least about 98%; and in yet other
embodiments, yield of .DELTA.8-THC and .DELTA.9-THC is between
about 75 to 98% or 99%.
[0346] In alternative embodiments, purity is determined by Gas
Chromatography-Mass Spectrometry (GC-MS) and/or by analytical
high-performance liquid chromatography (HPLC). The total ion
chromatogram from the GC-MS gives information similar to that
provided by a flame ionization detector (FID)-GC in that the peak
area is proportional to the mass of the analytes detected. Total
peak area and the peak areas of the individual analytes can be
compared in the GC-MS case as long as the masses are in generally
the same range. As discussed below, in some embodiments, purity of
the .DELTA.8-THC and .DELTA.9-THC mixture isolated by the process
is greater than about 90%, 95%, 97% or 98%, or purity is greater
that about 98% to 99%.
Chapter II
[0347] In alternative embodiments, provided are continuous
isolation and purification processes for preparing a substantially
pure cannabidiol or a product enriched in cannabidiol from
synthetic or natural sources, e.g., from plant or microbial
material or microbial extracts. In alternative embodiments,
provided herein are improved methods for converting cannabidiol
(CBD) to .DELTA..sup.9-THC, including a purification and conversion
process based on a simple combination of continuous chromatographic
gradient elutions and semi continuous isomerization reactions. This
exemplary process is simple, efficient and economic.
[0348] In alternative embodiments, provided are methods of
preparing cannabinoids in substantially pure form starting from
plant extract material and conversion of the purified CBD to form
both .DELTA..sup.8 THC and .DELTA..sup.9 THC and subsequent
conversion of the formed .DELTA..sup.8 tetrahydrocannabinol into
.DELTA..sup.9 tetrahydrocannabinol.
[0349] In alternative embodiments, provided are processes for
producing and isolating cannabinoids from cannabis and hemp
extracts which contain cannabinoids in minute amounts. In
alternative embodiments, provided are processes for producing and
isolating cannabinoids from natural materials, including plant or
plant extracts, microbes, or botanical drug substances, or
synthetically and semi-synthetically prepared cannabinoid products,
or from recombinantly engineered microbes, e.g., yeasts or bacteria
recombinantly engineered to express one or more cannabinoids. In
one embodiment, exemplary methods are inexpensive and provide
specific cannabinoid concentrates (e.g., of CBD, .DELTA..sup.8-THC,
.DELTA..sup.9-THC) of high purity.
[0350] In one embodiment, exemplary methods provide a simple and
economical continuous process for separating and concentrating
cannabinoids from solvent-extracted cannabinoid containing
materials. In one embodiment, exemplary methods provide a method
that first converts the substantially isolated CBD into a mixture
of .DELTA..sup.8-THC and .DELTA..sup.9-THC, and then subsequently
converts the .DELTA..sup.8-THC into .DELTA..sup.9-THC. In
alternative embodiments, the solvent-extracted cannabinoid
containing materials are derived from synthetic or biological
materials such as hemp and Cannabis or botanical drug substances,
or from microbial materials; and the solvent extraction methods can
be polar solvent extractions, nonpolar solvent extractions, or the
solvent extraction methods can comprise use of super critical
carbon dioxide or mixtures thereof. The solvent extraction methods
can extract cannabinoids substantially from the synthetic or
biological, e.g., plant, matter, along with other plant matter
comprising lipids, waxes, monoterpenes, sesquiterpenes,
hydrocarbons, alkaloids, flavonoids and chlorophylls.
[0351] In alternative embodiments, methods provided herein comprise
subjecting cannabinoid containing solvent extract starting
materials to a number of chromatographic resins in various
contacting steps using various gradient elution solvents s.
[0352] In alternative embodiments, the cannabinoids which can be
fractionated and isolated using methods as provided herein, or
which can be produced in reactions as provided herein, or from
which the solvent extracts are derived can be from, or can
comprise: .DELTA..sup.9tetrahydrocannabinol (.DELTA..sup.9 THC);
cannabidiol (CBD); cannabinol (CBN); cannabigerol (CBG);
cannabichromene (CBC); cannabidivarol (CBDV); tetrahydrocannabidiol
(THCBD); tetrahydrocannabigerol (THCBG); tetrahydrocannabichromene
(THCBC); tetrahydrocannabidivarol (THCBDV), or combinations
thereof, including carboxylic acid precursors of the foregoing
compounds and related naturally occurring compounds and their
derivatives.
[0353] In alternative embodiments, provides are methods of
preparing or obtaining a substantially pure cannabinoid or a
product enriched in a given cannabinoid comprising: [0354] (i)
obtaining an extract or extract solution comprising or containing a
cannabinoid or a cannabinoid acid from a natural or a synthetic
source, e.g., a plant or microbial material; [0355] (ii) filtering
the extract or extract solution of step (i) to remove solids and
color bodies; [0356] (iii) removing the extract solvent (as an
extract fraction); [0357] (v) continuously loading of an amount of
extract solution over a defined time increment over multiple
stationary phase resins columns such as a normal phase, reverse
phase and/or ion exchange chromatographic resin; [0358] (vi)
continuously eluting the extract solution using multiple defined
gradient elution solvents s at specific time increments and
volumes; [0359] (vii) continuously collecting the gradient elution
fractions; [0360] (viii) removal of the gradient elution solvent
from the produced fractions to generate a substantially purified
extract [0361] (ix) optionally loading of specific first gradient
elution fractions on a reverse phase, ion exchange or normal
chromatographic resin; [0362] (x) continuously eluting the first
gradient elution fractions with a second gradient elution solvent;
and, [0363] (xi) removing of the second gradient elution solvent
from the produced fractions to produce a purified extract.
[0364] In alternative embodiments, the methods further
comprise:
[0365] a step (xii): loading of first and second gradient elution
solvents s onto an ion exchange chromatographic resin;
[0366] a step (xiii): eluting an extract solution from the ion
exchange chromatographic resin of step (xi) using a gradient
solvent and collecting the gradient elution fractions; and
[0367] (xiv) removing the gradient elution solvent from the
produced fractions of step (xiii) to produce purified and
substantially purified extract.
[0368] In alternative embodiments, provided are methods of
converting CBD to .DELTA..sup.8 THC and .DELTA..sup.9 THC
comprising: [0369] (a) providing a reaction mixture comprising a
catalyst in an organic solvent; [0370] (b) adding CBD material from
steps (viii) and steps (xiii), above; [0371] (c) mixing said
reaction mixture; [0372] (d) allowing the mixture to separate into
an aqueous phase and an organic phase; [0373] optionally adding a
second solvent; and [0374] (e) removing the organic phase; and,
[0375] optionally, repeating steps (a) through (e).
[0376] In alternative embodiments, provided are methods for
purifying .DELTA..sup.8-THC and .DELTA..sup.9-THC comprising:
[0377] (a) providing a reaction mixture comprising a stabilizing
agent in an organic solvent; [0378] (b) adding .DELTA..sup.8-THC
and .DELTA..sup.9-THC to the reaction mixture; [0379] (c) mixing
said reaction mixture to form 9-chlorohexahydrocannabinol; [0380]
(d) combining said reaction mixture with a second organic solvent;
[0381] (e) adding an elimination catalyst or reagent to the
reaction mixture to cause an elimination refluxing said reaction
mixture under an inert atmosphere; [0382] (f) pouring the mixture
into cold water; [0383] (g) mixing the mixture; [0384] (h) allowing
the mixture to separate into an aqueous phase and an organic phase;
[0385] optionally adding a second solvent; [0386] (i) removing the
organic phase, which comprises a purified, or a substantially pure,
.DELTA..sup.8-THC and .DELTA..sup.9-THC; and [0387] optionally,
repeating steps (a) through (i).
[0388] In alternative embodiments, provided are methods for
converting CBD to substantially pure .DELTA..sup.9-THC comprising:
[0389] (a) providing a reaction mixture comprising a CBD in an
organic solvent; [0390] (b) adding a catalyst to the reaction
mixture under a nitrogen atmosphere; [0391] (c) stirring the
reaction mixture; [0392] (d) adding NaHCO.sub.3 or equivalent to
the reaction mixture; [0393] (e) allowing the mixture to separate
into an aqueous phase and an organic phase; [0394] optionally
adding a second solvent; [0395] (f) removing the organic phase,
which comprises the converted .DELTA..sup.9-THC; and optionally
repeating steps (a) through (f).
[0396] In alternative embodiments, a "substantially pure"
preparation of cannabinoid is defined as a preparation having a
chromatographic purity (of the desired cannabinoid or cannabinoid
acid) of greater than about 75%, or greater than about 96%, or
greater than about 97%, or greater than about 98%, or greater than
about 99%, or greater than about 99.5%, or between about 70% and
99.9%, as determined by area normalisation of an HPLC profile.
[0397] In alternative embodiments, the term "product enriched in a
given cannabinoid" encompasses preparations having at least about
50%, or greater than about 75%, or greater than about 90%, 95% or
98%, or between about 50% and 99.9%, chromatographic purity, for
the desired cannabinoid.
[0398] In alternative embodiments, a non-purified, or
non-substantially purified, product can comprise a greater
proportion of impurities, non-target materials and/or other
cannabinoids than a "substantially pure" preparation. The
cannabinoid can be (e.g., a cannabinoid purified or isolated by, or
made by a reaction of, a method as provided herein can be):
.DELTA..sup.9tetrahydrocannabinol (.DELTA..sup.9 THC); cannabidiol
(CBD); cannabinol (CBN); cannabigerol (CBG); cannabichromene (CBC);
cannabidivarol (CBDV); tetra-hydrocannabidiol (THCBD);
tetra-hydrocannabigerol (THCBG); tetra-hydrocannabichromene
(THCBC); or, tetra-hydrocannabidivarol (THCBDV); the carboxylic
acid precursors of the foregoing compounds; and related naturally
occurring compounds and their derivatives.
[0399] In alternative embodiments, the term "cannabinoids" e.g., a
cannabinoid purified or isolated by, or made by a reaction of a
process as provided herein, includes or refers to a family of
natural products that can contain a 1,1'-di-methyl-pyrane ring, a
variedly derivatized aromatic ring and/or a variedly unsaturated
cyclohexyl ring and their immediate chemical precursors.
[0400] In alternative embodiments, the term "cannflavins", e g , a
cannflavin purified or isolated by, or made by a reaction of a
process as provided herein, includes or refers to a family of
natural products that can contain a 1,4-pyrone ring fused to a
variedly derivatized aromatic ring and linked to a second variedly
derivatized aromatic ring.
[0401] In alternative embodiments, the term "Lewis acid" refers to
a powerful electron pair acceptor; and examples include but are by
no means limited to BF.sub.3Et.sub.2O (boron trifluoride ditheyl
etherate), p-toluenesulfonic acid and boron trifluoride.
[0402] In alternative embodiments, the term "non-oxidizing acid"
refers to hydrobromic, hydrochloric, hydrofluoric, acetic, benzoic,
chloroacetic, formic, phosphoric, sulfuric, trifluroacetic and
oxalic acids.
[0403] In alternative embodiments, the term "stabilizing agent"
refers to a zinc chloride, hydrogen chloride, methylene chloride,
simonkolleite, and mixtures thereof; and examples include but are
by no means limited to zinc chloride, hydrogen chloride, and
simonkolleite, and polymorphs and hydrates thereof.
[0404] In alternative embodiments, the term "essential oils", e.g.,
an essential oil used as a starting material in a process as
provided herein, or an essential oil that may be isolated by a
process as provided herein, includes or refers to a family of
natural products that can contain a multiple of the 5-membered
isoprene unit variedly substituted, often cyclized to form one or
more ring systems; they may also contain series of aldehydes and/or
ketones and esters of a variety of carboxylic acid substituted
compounds.
[0405] In alternative embodiments, provided are methods for
extracting and/or purifying cannabinoids from any plant material
extract known to contain such cannabinoids, cannflavins and
essential oils; and, optionally to purify cannflavins and to
optionally purify essential oils. In alternative embodiments, the
extract is passed through a series of chromatographic columns, for
example, a normal phase column, a reversed phase column or an ion
exchange column as a continuous simulated moving bed
configuration.
[0406] In one embodiment, the chromatographic column is arranged
for gradient elution fractioning using normal phase, reverse phase
and/or ion exchange chromatography. In one embodiment
.DELTA..sup.9-THC and CBD are fractionated out of the eluent. For
example, in one embodiment, as the extract is passed over the
column, .DELTA..sup.9-THC and CBD is differentially retained or
detained (e.g., reversibly bound) on the column. As a result, as
the extract comes off the column, the initial fractions eluted off
the column will be (substantially) free of .DELTA..sup.9-THC and
CBD. The fractions free of .DELTA..sup.9-THC and CBD are pooled,
thereby producing an extract with .DELTA..sup.9-THC and CBD
substantially removed (e.g., in alternative embodiments,
"substantially removed" or "substantially fractionated" means at
least 85%, 90%, 95%, 98%, 99% or 99.5% or more removed or
fractionated).
[0407] In some embodiments, (substantially) only .DELTA..sup.9-THC
is substantially fractionated out of the eluent. For example, in
one embodiment, as the extract or pooled fractions from a previous
normal phase, reverse phase and/or ion exchange elution are passed
over the column, .DELTA..sup.9-THC is differentially retained or
detained (e.g., reversibly bound) on the column. As a result, as
the extracts are pooled previous elution fractions comes off the
column, the initial fractions eluted off the column will be free of
.DELTA..sup.9-THC. These fractions free of .DELTA..sup.9-THC are
pooled, thereby producing an extract with .DELTA..sup.9-THC
substantially removed (e.g., in alternative embodiments,
"substantially removed" or "substantially fractionated" means at
least 85%, 90%, 95%, 98%, 99% or 99.5% or more removed or
fractionated).
[0408] In some embodiments, when using a continuous chromatography
apparatus or device, a series of columns are arranged (for example,
at least 2, 3, 4, 5, 6, 7 or 8 or more, or between 3 and 30,
columns are arranged) in a continuous rotation traveling through a
series of contact points where gradient elution solvents s and
extract solutions are introduced at fixed points for a period of
time allowing for continuous loading and elution, and collection of
fractions. The first column is loaded with extract solution at the
first position (or station). The first column is then moved to the
second position where the first gradient elution solvent is
introduced (or loaded) while at the same time a second column is
loaded with extract solution at position one. The first column then
rotates (i.e., is moved) to the third position where the second
gradient solvent is introduced, the second column moves to the
second position where the first gradient solvent is introduced and
a third column is loaded with extract solution at position one. The
first column then moves to the fourth position where the third
gradient solvent is introduced, the second column moves to the
third position where the second gradient solvent is introduced, the
third column moves to the second position where the first gradient
solvent is introduced and the fourth column is loaded with extract
solution at position one. The first column then moves to the fifth
position where the fourth gradient solvent is introduced, the
second column moves to the fourth position where the third gradient
solvent is introduced, the third column moves to the third position
where the second gradient solvent is introduced, the fourth column
moves to the second position where the first gradient solvent is
introduced and the fifth column is loaded with extract solution at
position one. The first column then moves to the sixth position
where the fifth gradient solvent solution is introduced, the second
column moves to the fifth position where the fourth gradient
solvent is introduced, the third column moves to the fourth
position where the third gradient solvent is introduced, the fourth
column moves to the third position where the second gradient
solvent is introduced, the fifth column moves to the second
position where the first gradient solvent is introduced and the
sixth column is loaded with extract solution at position one. The
first column then moves or returns to the first position where
extract solution is loaded, the second column moves to the sixth
position where the fifth gradient solvent introduced, the third
column moves to the fifth position where the fourth gradient
solvent is introduced, the fourth column moves to the fourth
position where the third gradient solvent is introduced, the fifth
column moves to the third position where the second gradient
solvent is introduced and the sixth column moves to the second
position where the first gradient solvent is introduced.
[0409] In an alternative embodiment, the first gradient solvent
elutes CBD, CBG and CBN and is substantially fractionated in the
eluent. For example, in one embodiment, as the extract or pooled
fractions from a normal phase, reverse phase and/or ion exchange
elution are passed over the column, CBD, CBG and CBN is
differentially produced in the eluent (e.g., by use of a gradient
elution process). In a particular embodiment second gradient
solvent elutes CBD and is substantially fractionated in the eluent.
For example, in one embodiment, as the extract or pooled fractions
from a normal phase, reverse phase and/or ion exchange elution are
passed over the column, CBD is differentially produced in the
eluent. In a particular embodiment the third gradient solvent
elutes CBD and .DELTA..sup.9-THC and is substantially fractionated
in the eluent. For example, in one embodiment, as the extract or
pooled fractions from a normal phase, reverse phase and/or ion
exchange elution are passed over the column, CBD and
.DELTA..sup.9-THC is differentially produced in the eluent. In a
particular embodiment the fourth gradient elutes .DELTA..sup.9-THC
and is substantially fractionated in the eluent. For example, in
one embodiment, as the extract or pooled fractions from a normal
phase, reverse phase and/or ion exchange elution are passed over
the column, .DELTA..sup.9-THC is differentially produced in the
eluent. In a particular embodiment the fifth gradient elutes CBC,
THC-A, terpenes and .DELTA..sup.9-THC and is substantially
fractionated in the eluent. For example, in one embodiment, as the
extract or pooled fractions from a normal phase, reverse phase
and/or ion exchange elution are passed over the column, CBC, THC-A,
terpenes and .DELTA..sup.9-THC is differentially produced in the
eluent.
[0410] In some embodiments, when using a continuous chromatography
apparatus or device, a series of columns are arranged, for example
3, 4, 5, 6, 7, 8 or more, or between 3 and 30 or more, columns or
more are arranged in a continuous rotation traveling through a
series of contact points where gradient elution solvents s and
extract solutions are introduced at fixed points for a period of
time allowing for continuous loading and elution, and collection of
fractions. The first column is loaded with extract solution at the
first position (or station). The first column is then moved to the
second position where the first gradient elution solvent is
introduced (or loaded) while at the same time the second column is
loaded with extract solution at position one. The first column then
rotates (i.e., is moved) to the third position where the second
gradient solvent is introduced, the second column moves to the
second position where the first gradient solvent is introduced and
the third column is loaded with extract solution at position one.
The first column then moves to the fourth position where the third
gradient solvent is introduced, the second column moves to the
third position where the second gradient solvent is introduced, the
third column moves to the second position where the first gradient
solvent is introduced and the fourth column is loaded with extract
solution at position one. The first column then moves to the fifth
position where the fourth gradient solvent i is introduced, the
second column moves to the fourth position where the third gradient
solvent is introduced, the third column moves to the third position
where the second gradient solvent is introduced, the fourth column
moves to the second position where the first gradient solvent is
introduced and the fifth column is loaded with extract solution at
position one. The first column then moves to the sixth position
where the fifth gradient solvent is introduced, the second column
moves to the fifth position where the fourth gradient solvent is
introduced, the third column moves to the fourth position where the
third gradient solvent is introduced, the fourth column moves to
the third position where the second gradient solvent is introduced,
the fifth column moves to the second position where the first
gradient solvent is introduced and the sixth column is loaded with
extract solution at position one. The first column then moves or
returns to the first position where extract solution is loaded, the
second column moves to the sixth position where the fifth gradient
solvent is introduced, the third column moves to the fifth position
where the fourth gradient solvent solution is introduced, the
fourth column moves to the fourth position where the third gradient
solvent is introduced, the fifth column moves to the third position
where the second gradient solvent is introduced and the sixth
column moves to the second position where the first gradient
solvent is introduced. The equipment is generally described in U.S.
Pat. Nos. 4,764,276, 4,808,317, and 4,710,364, for example, each of
which are incorporated herein by reference.
[0411] In a particular embodiment the first gradient solvent elutes
CBD, CBG and CBN and is substantially fractionated in the eluent.
For example, in one embodiment, as the extract or pooled fractions
from a normal phase, reverse phase and/or ion exchange elution are
passed over the column, CBD, CBG and CBN is differentially produced
in the eluent (e.g., by use of a gradient elution process). In a
particular embodiment second gradient solvent elutes CBD and is
substantially fractionated in the eluent. For example, in one
embodiment, as the extract or pooled fractions from a normal phase,
reverse phase and/or ion exchange elution are passed over the
column, CBD is differentially produced in the eluent. In a
particular embodiment the third gradient solvent elutes CBD and
.DELTA..sup.9-THC and is substantially fractionated in the eluent.
For example, in one embodiment, as the extract or pooled fractions
from a normal phase, reverse phase and/or ion exchange elution are
passed over the column, CBD and .DELTA..sup.9-THC is differentially
produced in the eluent. In a particular embodiment the fourth
gradient solvent elutes .DELTA..sup.9-THC and is substantially
fractionated in the eluent. For example, in one embodiment, as the
extract or pooled fractions from a normal phase, reverse phase
and/or ion exchange elution are passed over the column,
.DELTA..sup.9-THC is differentially produced in the eluent. In a
particular embodiment the fifth gradient solvent elutes CBC, THC-A,
terpenes and .DELTA..sup.9-THC and is substantially fractionated in
the eluent. For example, in one embodiment, as the extract or
pooled fractions from a normal phase, reverse phase and/or ion
exchange elution are passed over the column, CBC, THC-A, terpenes
and .DELTA..sup.9-THC is differentially produced in the eluent. The
second gradient solvent containing CBD is combined with a Lewis
acid or non-oxidizing acid catalyst, optionally additional CBD or
substantially pure CBD and/or (.DELTA..sup.8-THC) and/or
.DELTA..sup.9-tetrahydrocannabinol (.DELTA..sup.9-THC) can be added
to the reaction mixture, mixing said reaction mixture for a period
of time; adding a neutralizing agent to said mixture; filtration of
catalyst and neutralizing agent from mixture; optionally allowing
mixture to separate into an aqueous and organic phase; optionally
dissolving organic phase in a second solvent; adding a stabilizing
agent; mixing said reaction mixture for a period of time; adding a
elimination catalyst or reagent to said reaction mixture;
filtration of stabilizing agent and elimination agent from mixture;
optionally allowing mixture to separate into an aqueous phase and
an organic phase; optionally adding a further solvent; adding
organic phase to a chromatography column and eluting the
tetrahydrocannabinol from the organic phase. The
tetrahydrocannabinol may then be combined with suitable excipients
known in the art, thereby forming a pharmaceutical composition. In
one embodiment, the mixture is allowed to separate into an aqueous
phase and an organic phase; and optionally the process further
comprises removing the organic phase).
[0412] In some embodiments, (substantially) only CBD is
substantially fractionated out of the eluent. For example, in one
embodiment, as the extract or pooled fractions from a previous
normal phase, reverse phase and/or ion exchange elution are passed
over the column, CBD is differentially retained or detained (e.g.,
reversibly bound) on the column. As a result, as the extract or
pooled previous elution fractions comes off the column, the initial
fractions eluted off the column will be (substantially) free of
CBD. These fractions free of CBD are pooled, thereby producing an
extract with CBD substantially removed (e.g., in alternative
embodiments, "substantially removed" or "substantially
fractionated" means at least 85%, 90%, 95%, 98%, 99% or 99.5% or
more removed or fractionated).
[0413] In some embodiments, .DELTA..sup.9-THC carboxylic acid
species or .DELTA..sup.9-THCA and CBD carboxylic acid species CBDA
are fractionated out of out of the eluent. For example, in one
embodiment, as the extract or pooled fractions from a first elution
are passed over the column, .DELTA..sup.9-THCA and CBDA are
differentially retained or detained (e.g., reversibly bound) on the
column. As a result, as the extract or pooled first elution
fractions comes off the column, the initial fractions eluted off
the column will be (substantially) free of .DELTA..sup.9-THCA and
CBDA. These fractions (substantially) free of .DELTA..sup.9-THCA
and CBDA are pooled, thereby producing an extract with
.DELTA..sup.9-THCA and CBDA substantially removed (e.g., in
alternative embodiments, "substantially removed" or "substantially
fractionated" means at least 85%, 90%, 95%, 98%, 99% or 99.5% or
more removed or fractionated).
[0414] In some embodiments, the .DELTA..sup.9-THC may be eluted
from the column, extracted or concentrated, for purifying, or
substantially purifying, .DELTA..sup.9-THC. In alternative
embodiments, the chromatographic column is arranged for
fractionating (e.g., sequentially fractionating) a specific
cannabinoid or groups of cannabinoids or their carboxylic acid
species, cannflavin or essential oil or class of cannabinoids,
cannflavins or essential oils out of the eluent, for example,
cannabidiol (CBD), cannabinol (CBN), cannabigerol (CBG),
cannabichromene (CBC), cannabidivarol (CBDV), tetrahydrocannabidiol
(THCBD), tetrahydrocannabigerol (THCBG), tetrahydrocannabichromene
(THCBC), tetrahydrocannabidivarol (THCBDV), .DELTA..sup.8-THC, the
carboxylic acid precursors of the foregoing compounds, and related
naturally occurring compounds and their derivatives. In alternate
embodiments, the chromatographic column is arranged for
fractionating (e.g., sequentially fractionating) cannflavins and
related naturally occurring compounds and their derivatives. In
alternate embodiments, the system is arranged to fractionate the
components of essential oils. The list of compounds provided herein
is not exhaustive and is in no way intended to be limiting. In
these embodiments, the compound(s) of interest are retained or
detained (e.g., reversibly bound) on the column so that fractions
(alternatively, the last fractions) of the extract eluted from the
column contain the compounds(s) of interest. In alternative
embodiments, fractions containing the compound(s) of interest are
pooled. In some embodiments, different compounds may be extracted
with different solvents and then combined into a single extract. As
will be appreciated by one knowledgeable in the art, in this
manner, several different cannabinoids could be purified from a
single extract.
[0415] In alternative embodiments, the "plant or microbial
material" will be derived from one or more cannabis or hemp plants,
or from other plants, or microbes such as lichen, yeast, algae or
bacteria. The term "plant material" encompasses a plant or plant
part (e.g. bark, wood, leaves, stems, roots, flowers, fruits,
seeds, berries or parts thereof) as well as exudates, and includes
material falling within the definition of "botanical raw material"
in the Guidance for Industry Botanical Drug Products Draft
Guidance, August 2000, US Department of Health and Human Services,
Food and Drug Administration Centre for Drug Evaluation and
Research. The term "cannabis plant(s)" encompasses wild type
Cannabis sativa and also variants thereof, including Cannabis
chemovars (varieties characterized by virtue of chemical
composition) which naturally contain different amounts of the
individual cannabinoids, also Cannabis sativa subspecies indica
including the variants var. indica and var. kafiristanica, Cannabis
indica and also plants which are the result of genetic crosses,
self-crosses or hybrids thereof. The term "Cannabis plant material"
also can encompass plant material derived from one or more cannabis
plants, and can comprise any "cannabis plant material" including,
e.g., herbal Cannabis and dried Cannabis biomass. The term
"Cannabis plant material" also can encompass "decarboxylated
Cannabis plant material", which refers to Cannabis plant material
which has been subject to a decarboxylation step in order to
convert cannabinoid acids to the corresponding free
cannabinoids.
[0416] In alternative embodiments, a starting material for a
purification process as provided herein is an extract solution
containing a cannabinoid or cannabinoid acid obtained from a
natural or a synthetic source, e.g., a plant or microbial material.
In alternative embodiments, the "extract solution containing a
cannabinoid or cannabinoid acid" comprises a solvent extract of a
plant or microbial material. Solvents used for extraction for use
in the preparation of extract solutions can comprise non-polar
solvents, polar solvents such as ethanol, methanol or water, or
organic solvents such as liquid carbon dioxide, and combinations
thereof. The solvent may be an organic solvent, selected from the
group consisting of: non-polar solvents include liquid non-polar
solvents comprising lower C1-C12, preferably C3 to C8, straight
chain or branched chain alkanes for example, methane, ethane,
propane, butane, pentane, toluene, trimethylpentane; a low
molecular weight alcohol, polar solvents consisting of for example,
ethanol, methanol, water; a low molecular weight chlorinated
hydrocarbon, for example, chloroform and dichloromethane; or a
supercritical fluid such as CO.sub.2 with or without an organic
solvent modifier.
[0417] In alternative embodiments, the extract is prepared by
dissolving or partially dissolving the natural or synthetic, or the
plant or microbial material in a solvent, removing insoluble
material from the resultant solution (optionally by filtration with
or without activated carbon, precipitation, centrifugation and the
like), and optionally removing some or all of the extraction
solvent from the solution (optionally by rotary evaporation) to
form an extract or extract solution or concentrate containing a
cannabinoid or cannabinoid acid.
[0418] In alternative embodiments, extractions can comprise using a
technique referred to as accelerated solvent extraction or may use
subcritical water or any combination of water and solvent. In one
embodiment, when isolating cannabinoid acids, a modified pH
gradient elution solvent is used. The primary purpose of this pH
adjustment (the modified pH gradient) is to promote or prevent
ionization of the cannabinoid acid. pH modified gradient elution
solutions may be achieved by the additional of a small volume of
acid or base to the solvent. It may be sufficient to add a
relatively weak acid, such as acetic acid, oxalic acid, glycolic
acid, carbonic acid or ammonium hydroxide or a small amount of base
or buffering agent such as sodium hydroxide, magnesium hydroxide,
sodium carbonate or sodium bicarbonate. For any given purification
process the optimal amount and type of acid or base used may be
determined empirically. An alternative exemplary acidified solvent
is 0.1% acetic acid in ethanol or 0.1% sodium hydroxide in ethanol.
In alternative embodiments, the neutralizing agent consists of for
example sodium hydroxide, sodium carbonate, potassium carbonate,
and potassium t-amylate, sodium bicarbonate.
[0419] Acidified non-polar and polar solvents of the types
described above can be useful in preparation of gradient elutions
using ion exchange chromatography. The solvents used in the
conversion can comprise an organic solvent, e.g., a non-polar
solvent, including a liquid non-polar solvent comprising lower
C1-C12, or C3 to C8, straight chain or branched chain alkanes for
example, methane, ethane, propane, butane, pentane, toluene,
trimethylpentane, hexane; a low molecular weight alcohol, polar
solvents consisting of for example, ethanol; a low molecular weight
chlorinated hydrocarbon, for example, chloroform and
dichloromethane; a heterocyclic compound or cyclic ether for
example, tetrahydrofuran and 2-Methyltetrahydrofuran and aromatic
ring hydrocarbons such as benzene, toluene, xylene and
ethylbenzene.
[0420] In alternative embodiments, the elimination catalyst
consists of a catalyst and or reagent such as, for example, lithium
hydroxide (LiOH), sodium hydroxide (NaOH), potassium hydroxide
(KOH), rubidium hydroxide (RbOH), cesium hydroxide (CsOH),
magnesium hydroxide (Mg(OH).sub.2), calcium hydroxide
(Ca(OH).sub.2), strontium hydroxide (Sr(OH).sub.2), barium
hydroxide (Ba(OH).sub.2), potassium hydride and/or sodium hydride,
potassium tert-pentoxide, organic superbases, bispidines,
multicyclic polyamines, organometallic compounds of reactive
metals, wherein optionally the reactive metals comprise
organolithium, organo-magnesium, lithium diisopropylamide,
n-butyllithium and potassium tert-butoxide, potassium
tert-pentoxide, lithium nitride, sodium methoxide, sodium ethoxide,
ortho-diethynylbenzene dianion, meta-diethynylbenzene dianion,
para-diethynylbenzene dianion, lithium monoxide anion, and/or a
methyl anion.
[0421] In alternative embodiments, the plant or microbial material
is subjected to a decarboxylation step prior to solvent extraction.
The purpose of the decarboxylation step is to convert cannabinoid
acids present in the plant or microbial material to the
corresponding free cannabinoids. In alternative embodiments, the
decarboxylation is carried out by heating the plant or microbial
material to a defined temperature for a suitable length of time.
Decarboxylation of cannabinoid acids is a function of time and
temperature, thus at higher temperatures a shorter period of time
will be taken for complete decarboxylation of a given amount of
cannabinoid acid. In alternative embodiments selecting appropriate
conditions for decarboxylation consideration includes minimizing
thermal degradation of the desirable, pharmacological cannabinoids
into undesirable degradation products, particularly thermal
degradation of .DELTA..sup.9-THC. In alternative embodiments, the
decarboxylation is carried out in a multi-step heating process in
which the plant or microbial material is: i) heated to a first
temperature for a first (relatively short) time period to evaporate
off retained water and allow for uniform heating of the plant or
microbial material; and ii) the temperature is increased to a
second temperature for a second time period (typically longer than
the first time period) until at least 95% conversion of the acid
cannabinoids to their neutral form has occurred.
[0422] In alternative embodiments, the "extract containing a
cannabinoid or a cannabinoid acid" prepared from the starting plant
or microbial material comprises a "botanical drug substance"
prepared from the plant or microbial material, or a polar or
non-polar solvent solution of such a botanical drug substance. In
the context of this application a "botanical drug substance" is an
extract derived from plant or microbial material, which extract
fulfills the definition of "botanical drug substance" provided in
the Guidance for Industry Botanical Drug Products Draft Guidance,
August 2000, US Department of Health and Human Services, Food and
Drug Administration Centre for Drug Evaluation and Research of: "A
drug substance derived from one or more plants, algae, or
macroscopic fungi. It is prepared from botanical raw materials by
one or more of the following processes: pulverization, decoction,
expression, aqueous extraction, ethanolic extraction, or other
similar processes." "Botanical drug substances" derived from
cannabis plants include primary extracts prepared by such processes
as, for example, maceration, percolation, and solvent
extraction.
[0423] In alternative embodiments, solvent extraction may be
carried out using essentially any solvent that dissolves, or
substantially dissolves, cannabinoids/cannabinoid acids, such as
for example C1 to C5 alcohols (e.g. ethanol, methanol), C5-C12
alkanes (e.g. hexane), norflurane (HFA134a),
1,1,1,2,3,3,3-Heptafluoropropane (or HFA227), chloroform,
dichloromethane, dichloroethane and carbon dioxide. When solvents
such as those listed above are used, the resultant extract
typically contains non-specific lipid-soluble material. This can
optionally be removed by a variety of processes including
filtration to remove solids, "winterization", which involves for
example chilling to -20.degree. C. or lower followed by filtration
to remove waxy ballast, extraction with liquid carbon dioxide and
by distillation.
[0424] In alternative embodiments, any protocol for the preparation
of botanical drug substances from cannabis and hemp plant material
can be used, e.g., as described in International patent application
WO 02/064109. In alternative embodiments, the botanical drug
substance is obtained by carbon dioxide (CO.sub.2) extraction,
polar solvent extraction or non-polar solvent extraction or
combinations thereof followed by a filtration. Optionally a
secondary extraction is performed to remove a substantial
proportion of non-cannabinoid materials, e.g. waxes, wax esters and
glycerides, unsaturated fatty acid residues, terpenes, carotenes,
and flavonoids and other ballast.
[0425] In alternative embodiments, if it is intended to prepare
free cannabinoids from the plant or microbial material, e.g.,
cannabis, then the material is heated to a defined temperature for
a defined period of time in order to partially or substantially
decarboxylate cannabinoid acids to free cannabinoids prior to
extraction of the botanical drug substance. In alternative
embodiments, the botanical drug substance is prepared according to
a process comprising the following steps: i) optional
decarboxylation of the plant or microbial material, ii) extraction
with polar or non-polar solvent, to produce a crude botanical drug
substance, iii) optional precipitation with C1-C5 alcohol to reduce
the proportion of non-target materials, iv) removal of the
precipitate (preferably by filtration), v) optional treatment with
activated charcoal, and vi) evaporation to remove C1-C5 alcohol and
water, thereby producing a final botanical drug substance.
[0426] In alternative embodiments, provided are methods for
converting first substantially purified cannabidiol (CBD) to
.DELTA..sup.8-tetrahydrocannabinol (.DELTA..sup.8-THC) and
.DELTA..sup.9-tetrahydrocannabinol (.DELTA..sup.9-THC), and then
further converting the produced .DELTA.9-tetrahydrocannabinol
(.DELTA..sup.9-THC) to .DELTA..sup.8-tetrahydrocannabinol
(.DELTA..sup.8-THC). As will be appreciated by one knowledgeable in
the art and as discussed below, the reaction times may be varied
somewhat, producing product at different yields and purities.
Furthermore, functional equivalents may be substituted where
appropriate.
[0427] In alternative embodiments, an exemplary method of
converting CBD to .DELTA..sup.8-tetrahydrocannabinol
(.DELTA..sup.8-THC) and .DELTA..sup.9-tetrahydrocannabinol
(.DELTA..sup.9-THC) and further converting (.DELTA..sup.9-THC) to
(.DELTA..sup.8-THC) comprises: providing a reaction mixture
comprising a Lewis acid or non-oxidizing acid catalyst in a
reaction solvent, adding a substantially pure CBD or substantially
pure CBD and/or (.DELTA..sup.8-THC) and/or
.DELTA..sup.9-tetrahydrocannabinol (.DELTA..sup.9-THC) to the
reaction mixture, mixing said reaction mixture for a period of
time; adding a neutralizing agent to said mixture; filtration of
catalyst and neutralizing agent from mixture; optionally allowing
mixture to separate into an aqueous and organic phase; optionally
dissolving organic phase in a second solvent; adding a stabilizing
agent; mixing said reaction mixture for a period of time; adding a
elimination catalyst or reagent to said reaction mixture;
filtration of stabilizing agent and elimination agent from mixture;
optionally allowing mixture to separate into an aqueous phase and
an organic phase; optionally adding a further solvent; adding
organic phase to a chromatography column and eluting the
tetrahydrocannabinol from the organic phase. The
tetrahydrocannabinol may then be combined with suitable excipients
known in the art, thereby forming a pharmaceutical composition. In
one embodiment, the mixture is allowed to separate into an aqueous
phase and an organic phase; and optionally the process further
comprises removing the organic phase.
[0428] In alternative embodiments, the tetrahydrocannabinol at
therapeutically effective concentrations or dosages is combined
with a pharmaceutically or pharmacologically acceptable carrier,
excipient or diluent, either biodegradable or non-biodegradable.
Exemplary examples of carriers include, but are by no means limited
to, for example, poly(ethylene-vinyl acetate), copolymers of lactic
acid and glycolic acid, poly(lactic acid), gelatin, collagen
matrices, polysaccharides, poly(D,L lactide), poly(malic acid),
poly(caprolactone), celluloses, albumin, starch, casein, dextran,
polyesters, ethanol, methacrylate, polyurethane, polyethylene,
vinyl polymers, glycols, mixtures thereof and the like. Standard
excipients that can be used include gelatin, casein, lecithin, gum
acacia, cholesterol, tragacanth, stearic acid, benzalkonium
chloride, calcium stearate, glyceryl monostearate, cetostearyl
alcohol, cetomacrogol emulsifying wax, sorbitan esters,
polyoxyethylene alkyl ethers, polyoxyethylene castor oil
derivatives, polyoxyethylene sorbitan fatty acid esters,
polyethylene glycols, polyoxyethylene stearates, colloidol silicon
dioxide, phosphates, sodium dodecyl sulfate, carboxymethylcellulose
calcium, carboxymethylcellulose sodium, methylcellulose,
hydroxyethylcellulose, hydroxypropylcellulose,
hydroxypropylmethycellulose phthalate, non-crystalline cellulose,
magnesium aluminum silicate, triethanolamine, polyvinyl alcohol,
polyvinylpyrrolidone, sugars and starches. See, for example,
Remington: The Science and Practice of Pharmacy, 1995, Gennaro
ed.
[0429] In some embodiments, the catalyst is a Lewis acid, for
example, p-toluenesulfonic acid, boron trifluoride or
BF.sub.3Et.sub.2O. In some embodiments, the BF.sub.3Et.sub.2O
(boron trifluoride diethyl etherate) is in dry methylene chloride,
ethylethyl acetate, ethanol, hexane or other organic solvent. In
yet other examples, the catalyst may be hydrochloric acid in
ethanol or sulfuric acid in cyclohexane.
[0430] In some embodiments, the catalyst is a non-oxidizing acid,
for example, formic acid, acetic acid or hydrobromic acid. In some
embodiments, the non-oxidizing acid is in dry methylene chloride,
ethyl acetate, ethanol, hexane or other organic solvent.
[0431] In some embodiments, a base is added to the reaction mixture
prior to allowing the reaction mixture to separate into organic and
aqueous phases. The base may be an alkali metal hydrogen carbonate,
carbonate of an alkali metal, lithium hydroxide (LiOH), sodium
hydroxide (NaOH), potassium hydroxide (KOH), rubidium hydroxide
(RbOH), cesium hydroxide (CsOH), magnesium hydroxide (Mg(OH)),
calcium hydroxide (Ca(OH)), strontium hydroxide (Sr(OH)), barium
hydroxide (Ba(OH)).
[0432] In some embodiments, the organic layer is dried prior to
eluting. In these embodiments, a suitable drying or dehydration
compound, for example, MgSO4 or Na.sub.2SO.sub.4 is used.
[0433] In yet other embodiments, the process may be carried out
under a nitrogen (e.g., N.sub.2) atmosphere.
[0434] In alternative embodiments, and as discussed below, yield is
determined by looking at the peak area for the isolated compound in
the gas chromatography-mass spectra analysis of the crude reaction
product mixture and the final reaction product mixture. It is
important to note that in the prior art, yield is often calculated
on the basis of first isolated crude product before final
purification. In some embodiments of processes provided herein
yield of .DELTA.9-THC is at least about 75%; in other embodiments,
the yield of .DELTA.9-THC is at least about 90%; and in other
embodiments, yield of .DELTA.9-THC is at least about 98%; and in
yet other embodiments, yield of .DELTA.9-THC is between about 75 to
98% or 99%.
[0435] In alternative embodiments, purity is determined by Gas
Chromatography-Mass Spectrometry (GC-MS) and/or by analytical
high-performance liquid chromatography (HPLC). The total ion
chromatogram from the GC-MS gives information similar to that
provided by a flame ionization detector (FID)-GC in that the peak
area is proportional to the mass of the analytes detected. Total
peak area and the peak areas of the individual analytes can be
compared in the GC-MS case as long as the masses are in generally
the same range. As discussed below, in some embodiments, purity of
the .DELTA..sup.9-THC isolated by the process is greater than about
70%, 80%, 90%, 95%, 97% or 98% or more, or purity is greater that
about 98% to 99% or 90% to 99.5%.
Chapter III
[0436] In alternative embodiments, provided are continuous
isolation and purification processes for preparing a substantially
pure cannabidiol or a product enriched in cannabidiol from plant or
microbial material extracts. In alternative embodiments, provided
herein are improved methods for converting cannabidiol (CBD) to
.DELTA..sup.8-THC, including a purification and conversion process
based on a simple combination of continuous chromato-graphic
gradient elutions and semi continuous isomerization reactions. This
exemplary process is simple, efficient and economic.
[0437] In alternative embodiments, provided are methods of
continuous isolation of cannabinoids in substantially pure form
starting from plant extract material and conversion of the purified
CBD to form both .DELTA..sup.8 THC and .DELTA..sup.9 THC and
subsequent conversion of the formed .DELTA..sup.9
tetrahydrocannabinol into A.sup.8 tetrahydrocannabinol.
[0438] In alternative embodiments, provided are processes for
producing and isolating cannabinoids from cannabis and hemp
extracts which contain cannabinoids in minute amounts. In
alternative embodiments, provided are processes for producing and
isolating cannabinoids from natural materials, including plant or
plant extracts, microbes, or botanical drug substances, or
synthetically and semi-synthetically prepared cannabinoid products,
or from recombinantly engineered microbes, e.g., yeasts or bacteria
recombinantly engineered to express one or more cannabinoids. In
one embodiment, exemplary methods are inexpensive and provide
specific cannabinoid concentrates (e.g., of CBD, .DELTA..sup.8-THC,
.DELTA..sup.9-THC) of high purity.
[0439] In one embodiment, exemplary methods provide a simple and
economical continuous process for separating and concentrating
cannabinoids from solvent-extracted cannabinoid containing
materials. In one embodiment, exemplary methods provide a method
that first converts the substantially isolated CBD into a mixture
of .DELTA..sup.8-THC and .DELTA..sup.9-THC, and then subsequently
converts the .DELTA..sup.9-THC into .DELTA..sup.8-THC. In
alternative embodiments, the solvent-extracted cannabinoid
containing materials are derived from synthetic or biological
materials such as hemp and Cannabis or botanical drug substances,
or from microbial materials; and the solvent extraction methods can
be polar solvent extractions, nonpolar solvent extractions, or the
solvent extraction methods can comprise use of super critical
carbon dioxide or mixtures thereof. The solvent extraction methods
can extract cannabinoids substantially from the synthetic or
biological, e.g., plant, matter, along with other plant matter
comprising lipids, waxes, monoterpenes, sesquiterpenes,
hydrocarbons, alkaloids, flavonoids and chlorophylls.
[0440] In alternative embodiments, methods provided herein comprise
subjecting cannabinoid containing solvent extract starting
materials to a number of chromatographic resins in various
contacting steps using various gradient elution solutions.
[0441] In alternative embodiments, the cannabinoids which can be
fractionated and isolated using methods as provided herein, or
which can be produced in reactions as provided herein, or from
which the solvent extracts are derived can be from, or can
comprise: .DELTA..sup.9tetrahydrocannabinol (.DELTA..sup.9 THC);
cannabidiol (CBD); cannabinol (CBN); cannabigerol (CBG);
cannabichromene (CBC); cannabidivarol (CBDV); tetrahydrocannabidiol
(THCBD); tetrahydrocannabigerol (THCBG); tetrahydrocannabichromene
(THCBC); tetrahydrocannabidivarol (THCBDV), or combinations
thereof, including carboxylic acid precursors of the foregoing
compounds and related naturally occurring compounds and their
derivatives.
[0442] In alternative embodiments, provides are methods of
preparing or obtaining a substantially pure cannabinoid or a
product enriched in a given cannabinoid comprising:
[0443] 1(i) obtaining an extract or extract solution containing a
cannabinoid or a cannabinoid acid from a plant or microbial
material; [0444] (ii) filtering the extract of step (i) to remove
solids and color bodies; [0445] (iii) removing the extract solvent
(as an extract fraction); [0446] (v) continuously loading of an
amount of extract solution over a defined time increment over
multiple stationary phase resins columns such as a normal phase,
reverse phase and/or ion exchange chromatographic resin; [0447]
(vi) continuously eluting the extract solution using multiple
defined gradient elution solutions at specific time increments and
volumes; [0448] (vii) continuously collecting the gradient elution
fractions; [0449] (viii) removal of the gradient elution solvent
from the produced fractions to generate a substantially purified
extract [0450] (ix) optionally loading of specific first gradient
elution fractions on a reverse phase, ion exchange or normal
chromatographic resin; [0451] (x) continuously eluting the first
gradient elution fractions with a second gradient elution solvent;
and, [0452] (xi) removing of the second gradient elution solvent
from the produced fractions to produce a purified extract.
[0453] In alternative embodiments, the methods further
comprise:
[0454] a step (xii): loading of first and second gradient elution
solvents s onto an ion exchange chromatographic resin;
[0455] a step (xiii): eluting an extract solution from the ion
exchange chromatographic resin of step (xi) using a gradient
solvent and collecting the gradient elution fractions; and
[0456] (xiv) removing the gradient elution solution from the
produced fractions of step (xiii) to produce purified and
substantially purified extract.
[0457] In alternative embodiments, provided are methods of
converting CBD to .DELTA..sup.8 THC and .DELTA..sup.9 THC
comprising: [0458] (a) providing a reaction mixture comprising a
catalyst in an organic solvent; [0459] (b) adding CBD material from
steps (viii) and steps (xiii), above; [0460] (c) mixing said
reaction mixture; [0461] (d) allowing the mixture to separate into
an aqueous phase and an organic phase; [0462] optionally adding a
second solvent; and [0463] (e) removing the organic phase; and,
[0464] optionally, repeating steps (a) through (e).
[0465] In alternative embodiments, provided are methods for
converting CBD to substantially pure A.sup.8-THC comprising: [0466]
(a) providing a reaction mixture comprising a CBD in an organic
solvent; [0467] (b) adding a catalyst to the reaction mixture under
a nitrogen atmosphere; [0468] (c) stirring the reaction mixture;
[0469] (d) controlling the reaction temperature and continuously
measuring the rate of reaction by observing conversion of CBD to
first .DELTA..sup.9-THC and second order of reaction
.DELTA..sup.8-THC; [0470] (d) adding NaHCO.sub.3 or equivalent to
the reaction mixture once .DELTA..sup.9-THC has be substantially
converted to .DELTA..sup.8-THC; [0471] (e) allowing the mixture to
separate into an aqueous phase and an organic phase and optionally
adding a second solvent; [0472] (f) removing the organic phase,
which comprises substantially the converted .DELTA..sup.8-THC; and
[0473] optionally repeating steps (a) through (f); [0474]
optionally performing steps (v) through (xiv).
[0475] In alternative embodiments, a "substantially pure"
preparation of cannabinoid is defined as a preparation having a
chromatographic purity (of the desired cannabinoid or cannabinoid
acid) of greater than about 75%, or greater than about 96%, or
greater than about 97%, or greater than about 98%, or greater than
about 99%, or greater than about 99.5%, or between about 70% and
99.9%, as determined by area normalisation of an HPLC profile.
[0476] In alternative embodiments, the term "product enriched in a
given cannabinoid" encompasses preparations having at least about
50%, or greater than about 75%, or greater than about 90%, 95% or
98%, or between about 50% and 99.9%, chromatographic purity, for
the desired cannabinoid.
[0477] In alternative embodiments, a non-purified, or
non-substantially purified, product can comprise a greater
proportion of impurities, non-target materials and/or other
cannabinoids than a "substantially pure" preparation. The
cannabinoid can be (e.g., a cannabinoid purified or isolated by, or
made by a reaction of, a method as provided herein can be):
.DELTA..sup.9tetrahydrocannabinol (.DELTA..sup.9 THC); cannabidiol
(CBD); cannabinol (CBN); cannabigerol (CBG); cannabichromene (CBC);
cannabidivarol (CBDV); tetra-hydrocannabidiol (THCBD);
tetra-hydrocannabigerol (THCBG); tetra-hydrocannabichromene
(THCBC); or, tetra-hydrocannabidivarol (THCBDV); the carboxylic
acid precursors of the foregoing compounds; and related naturally
occurring compounds and their derivatives.
[0478] In alternative embodiments, the term "cannabinoids" e.g., a
cannabinoid purified or isolated by, or made by a reaction of a
process as provided herein, includes or refers to a family of
natural products that can contain a 1,1'-di-methyl-pyrane ring, a
variedly derivatized aromatic ring and/or a variedly unsaturated
cyclohexyl ring and their immediate chemical precursors.
[0479] In alternative embodiments, the term "cannflavins", e.g., a
cannflavin purified or isolated by, or made by a reaction of a
process as provided herein, includes or refers to a family of
natural products that can contain a 1,4-pyrone ring fused to a
variedly derivatized aromatic ring and linked to a second variedly
derivatized aromatic ring.
[0480] In alternative embodiments, the term "Lewis acid" refers to
a powerful electron pair acceptor; and examples include but are by
no means limited to BF.sub.3Et.sub.2O (boron trifluoride ditheyl
etherate), p-toluenesulfonic acid and boron trifluoride.
[0481] In alternative embodiments, the term "non-oxidizing acid"
refers to hydrobromic, hydrochloric, hydrofluoric, acetic, benzoic,
chloroacetic, formic, phosphoric, sulfuric, trifluroacetic and
oxalic acids.
[0482] In alternative embodiments, the term "stabilizing agent"
refers to a zinc chloride, hydrogen chloride, methylene chloride,
simonkolleite, and mixtures thereof; and examples include but are
by no means limited to zinc chloride, hydrogen chloride,
simonkolleite, and polymorphs and hydrates thereof.
[0483] In alternative embodiments, the term "essential oils", e g ,
an essential oil used as a starting material in a process as
provided herein, or an essential oil that may be isolated by a
process as provided herein, includes or refers to a family of
natural products that can contain a multiple of the isoprene unit
variedly substituted, often cyclized to form one or more ring
systems; they may also contain series of aldehydes and/or ketones
and esters of a variety of carboxylic acid substituted
compounds.
[0484] In alternative embodiments, provided are methods for
extracting and/or purifying cannabinoids from any plant or
microbial material extract known to contain such cannabinoids,
cannflavins and essential oils; and, optionally to purify
cannflavins and to optionally purify essential oils. In alternative
embodiments, the extract is passed through a series of
chromatographic columns, for example, a normal phase column, a
reversed phase column or an ion exchange column as a continuous
column configuration.
[0485] In one embodiment, the chromatographic column is arranged
for gradient elution fractioning using normal phase, reverse phase
and/or ion exchange chromatography. In one embodiment
.DELTA..sup.9-THC and .DELTA..sup.8-THC and CBD are fractionated
out of the eluent. For example, in one embodiment, as the extract
is passed over the column, .DELTA..sup.9-THC, .DELTA..sup.8-THC and
CBD is differentially retained or detained (e.g., reversibly bound)
on the column. As a result, as the extract comes off the column,
the initial fractions eluted off the column will be (substantially)
free of .DELTA..sup.8-THC and CBD. The fractions free of
.DELTA..sup.8-THC and CBD are pooled, thereby producing an extract
with .DELTA..sup.9-THC, .DELTA..sup.8-THC and CBD substantially
removed (e.g., in alternative embodiments, "substantially removed"
or "substantially fractionated" means at least 85%, 90%, 95%, 98%,
99% or 99.5% or more removed or fractionated).
[0486] In some embodiments, (substantially) only .DELTA..sup.8-THC
is substantially fractionated out of the eluent. For example, in
one embodiment, as the extract or pooled fractions from a previous
normal phase, reverse phase and/or ion exchange elution are passed
over the column, .DELTA..sup.8-THC is differentially retained or
detained (e.g., reversibly bound) on the column. As a result, as
the extracts are pooled previous elution fractions comes off the
column, the initial fractions eluted off the column will be free of
.DELTA..sup.8-THC. These fractions free of .DELTA..sup.8-THC are
pooled, thereby producing an extract with .DELTA..sup.9-THC
substantially removed (e.g., in alternative embodiments,
"substantially removed" or "substantially fractionated" means at
least 85%, 90%, 95%, 98%, 99% or 99.5% or more removed or
fractionated).
[0487] In some embodiments, when using a continuous chromatography
apparatus or device, a series of columns are arranged, for example
3, 4, 5, 6, 7, 8 or more, or between 3 and 30 or more, columns are
arranged in a continuous rotation traveling through a series of
contact points where gradient elution solutions and extract
solution are introduced at fixed points for a period of time
allowing for continuous loading and elution, and collection of
fractions. The first column is loaded with extract solution at the
first position (or station). The first column is then moved to the
second position where the first gradient elution is introduced (or
loaded) while at the same time the second column is loaded with
extract solution at position one. The first column then rotates
(i.e., is moved) to the third position where the second gradient
solvent is introduced, the second column moves to the second
position where the first gradient solvent is introduced and the
third column is loaded with extract solution at position one. The
first column then moves to the fourth position where the third
gradient solvent is introduced, the second column moves to the
third position where the second gradient solvent is introduced, the
third column moves to the second position where the first gradient
solvent is introduced and the fourth column is loaded with extract
solution at position one. The first column then moves to the fifth
position where the fourth gradient solvent is introduced, the
second column moves to the fourth position where the third gradient
solvent is introduced, the third column moves to the third position
where the second gradient solvent is introduced, the fourth column
moves to the second position where the first gradient solvent is
introduced and the fifth column is loaded with extract solution at
position one. The first column then moves to the sixth position
where the fifth gradient solvent is introduced, the second column
moves to the fifth position where the fourth gradient solvent is
introduced, the third column moves to the fourth position where the
third gradient solvent is introduced, the fourth column moves to
the third position where the second gradient solvent is introduced,
the fifth column moves to the second position where the first
gradient solvent is introduced and the sixth column is loaded with
extract solution at position one. The first column then moves or
returns to the first position where extract solution is loaded, the
second column moves to the sixth position where the fifth gradient
solvent is introduced, the third column moves to the fifth position
where the fourth gradient solvent is introduced, the fourth column
moves to the fourth position where the third gradient solvent is
introduced, the fifth column moves to the third position where the
second gradient solvent is introduced and the sixth column moves to
the second position where the first gradient solvent is introduced.
The equipment is generally described in U.S. Pat. Nos. 4,764,276,
4,808,317, and 4,710,364, for example, each of which are
incorporated herein by reference.
[0488] In a particular embodiment the first gradient solvent elutes
CBD, CBG and CBN and is substantially fractionated in the eluent.
For example, in one embodiment, as the extract or pooled fractions
from a normal phase, reverse phase and/or ion exchange elution are
passed over the column, CBD, CBG and CBN is differentially produced
in the eluent (e.g., by use of a gradient elution process). In a
particular embodiment second gradient solvent elutes CBD and is
substantially fractionated in the eluent. For example, in one
embodiment, as the extract or pooled fractions from a normal phase,
reverse phase and/or ion exchange elution are passed over the
column, CBD is differentially produced in the eluent. In a
particular embodiment the third gradient solvent elutes CBD and
.DELTA..sup.9-THC and is substantially fractionated in the eluent.
For example, in one embodiment, as the extract or pooled fractions
from a normal phase, reverse phase and/or ion exchange elution are
passed over the column, CBD and .DELTA..sup.9-THC is differentially
produced in the eluent. In a particular embodiment the fourth
gradient elutes .DELTA..sup.9-THC and is substantially fractionated
in the eluent. For example, in one embodiment, as the extract or
pooled fractions from a normal phase, reverse phase and/or ion
exchange elution are passed over the column, .DELTA..sup.9-THC is
differentially produced in the eluent. In a particular embodiment
the fifth gradient elutes CBC, THC-A, terpenes and
.DELTA..sup.9-THC and is substantially fractionated in the eluent.
For example, in one embodiment, as the extract or pooled fractions
from a normal phase, reverse phase and/or ion exchange elution are
passed over the column, CBC, THC-A, terpenes and .DELTA..sup.9-THC
is differentially produced in the eluent.
[0489] In some embodiments, when using a continuous chromatography
apparatus or device, a series of columns are arranged for example
3, 4, 5, 6, 7, 8 or more, or between 3 and 30 or more, columns are
arranged in a continuous rotation traveling through a series of
contact points where gradient elution solutions and extract
solution are introduced at fixed points for a period of time
allowing for continuous loading and elution, and collection of
fractions. The first column is loaded with extract solution at the
first position (or station). The first column is then moved to the
second position where the first gradient elution is introduced (or
loaded) while at the same time the second column is loaded with
extract solution at position one. The first column then rotates
(i.e., is moved) to the third position where the second gradient
solvent solution is introduced, the second column moves to the
second position where the first gradient solvent is introduced and
the third column is loaded with extract solution at position one.
The first column then moves to the fourth position where the third
gradient solvent is introduced, the second column moves to the
third position where the second gradient solvent is introduced, the
third column moves to the second position where the first gradient
solvent is introduced and the fourth column is loaded with extract
solution at position one. The first column then moves to the fifth
position where the fourth gradient solvent is introduced, the
second column moves to the fourth position where the third gradient
solvent is introduced, the third column moves to the third position
where the second gradient solvent is introduced, the fourth column
moves to the second position where the first gradient solvent is
introduced and the fifth column is loaded with extract solution at
position one. The first column then moves to the sixth position
where the fifth gradient solvent is introduced, the second column
moves to the fifth position where the fourth gradient solvent is
introduced, the third column moves to the fourth position where the
third gradient solvent is introduced, the fourth column moves to
the third position where the second gradient solvent is introduced,
the fifth column moves to the second position where the first
gradient solvent is introduced and the sixth column is loaded with
extract solution at position one. The first column then moves or
returns to the first position where extract solution is loaded, the
second column moves to the sixth position where the fifth gradient
solvent is introduced, the third column moves to the fifth position
where the fourth gradient solvent is introduced, the fourth column
moves to the fourth position where the third gradient solvent is
introduced, the fifth column moves to the third position where the
second gradient solvent is introduced and the sixth column moves to
the second position where the first gradient solvent is introduced.
In a particular embodiment the first gradient solvent elutes CBD,
CBG and CBN and is substantially fractionated in the eluent. For
example, in one embodiment, as the extract or pooled fractions from
a normal phase, reverse phase and/or ion exchange elution are
passed over the column, CBD, CBG and CBN is differentially produced
in the eluent (e.g., by use of a gradient elution process). In a
particular embodiment second gradient solvent elutes CBD and is
substantially fractionated in the eluent. For example, in one
embodiment, as the extract or pooled fractions from a normal phase,
reverse phase and/or ion exchange elution are passed over the
column, CBD is differentially produced in the eluent. In a
particular embodiment the third gradient solvent elutes CBD and
.DELTA..sup.9-THC and is substantially fractionated in the eluent.
For example, in one embodiment, as the extract or pooled fractions
from a normal phase, reverse phase and/or ion exchange elution are
passed over the column, CBD and .DELTA..sup.9-THC is differentially
produced in the eluent. In a particular embodiment the fourth
gradient elutes .DELTA..sup.9-THC and is substantially fractionated
in the eluent. For example, in one embodiment, as the extract or
pooled fractions from a normal phase, reverse phase and/or ion
exchange elution are passed over the column, .DELTA..sup.9-THC is
differentially produced in the eluent. In a particular embodiment
the fifth gradient elutes CBC, THC-A, terpenes and
.DELTA..sup.9-THC and is substantially fractionated in the eluent.
For example, in one embodiment, as the extract or pooled fractions
from a normal phase, reverse phase and/or ion exchange elution are
passed over the column, CBC, THC-A, terpenes and .DELTA..sup.9-THC
is differentially produced in the eluent. The second gradient
solvent containing CBD is combined with a Lewis acid or
non-oxidizing acid catalyst, optionally additional CBD or
substantially pure CBD and/or .DELTA.9-tetrahydrocannabinol
(.DELTA.9-THC) can be added to the reaction mixture, mixing said
reaction mixture for a period of time at a controlled temperature
to first convert CBD to .DELTA..sup.9-THC and continue to the
second order reaction to convert formed .DELTA..sup.9-THC to second
order reaction .DELTA..sup.8-THC ; adding a neutralizing agent to
said mixture once substantial conversion of .DELTA..sup.9-THC to
.DELTA..sup.8-THC is observed by sample analysis such as TLC, HPLC
or GCMS; filtration of catalyst and neutralizing agent from
mixture; optionally allowing mixture to separate into an aqueous
and organic phase; optionally adding a further solvent; adding
organic phase to a continuous or fixed chromatography column and
eluting the .DELTA..sup.8-THC tetrahydrocannabinol from the organic
phase. The tetrahydrocannabinol may then be combined with suitable
excipients known in the art, thereby forming a pharmaceutical
composition. In one embodiment, the mixture is allowed to separate
into an aqueous phase and an organic phase; and optionally the
process further comprises removing the organic phase.
[0490] In some embodiments, (substantially) only CBD is
substantially fractionated out of the eluent. For example, in one
embodiment, as the extract or pooled fractions from a previous
normal phase, reverse phase and/or ion exchange elution are passed
over the column, CBD is differentially retained or detained (e.g.,
reversibly bound) on the column. As a result, as the extract or
pooled previous elution fractions comes off the column, the initial
fractions eluted off the column will be (substantially) free of
CBD. These fractions free of CBD are pooled, thereby producing an
extract with CBD substantially removed (e.g., in alternative
embodiments, "substantially removed" or "substantially
fractionated" means at least 85%, 90%, 95%, 98%, 99% or 99.5% or
more removed or fractionated).
[0491] In some embodiments, .DELTA..sup.9-THC carboxylic acid
species or .DELTA..sup.9-THCA and CBD carboxylic acid species CBDA
are fractionated out of out of the eluent. For example, in one
embodiment, as the extract or pooled fractions from a first elution
are passed over the column, .DELTA..sup.9-THCA and CBDA are
differentially retained or detained (e.g., reversibly bound) on the
column. As a result, as the extract or pooled first elution
fractions comes off the column, the initial fractions eluted off
the column will be (substantially) free of .DELTA..sup.9-THCA and
CBDA. These fractions (substantially) free of .DELTA..sup.9-THCA
and CBDA are pooled, thereby producing an extract with
.DELTA..sup.9-THCA and CBDA substantially removed (e.g., in
alternative embodiments, "substantially removed" or "substantially
fractionated" means at least 85%, 90%, 95%, 98%, 99% or 99.5% or
more removed or fractionated).
[0492] In some embodiments, the .DELTA..sup.9-THC may be eluted
from the column, extracted or concentrated, for purifying, or
substantially purifying, .DELTA..sup.9-THC. In alternative
embodiments, the chromatographic column is arranged for
fractionating (e.g., sequentially fractionating) a specific
cannabinoid or groups of cannabinoids or their carboxylic acid
species, cannflavin or essential oil or class of cannabinoids,
cannflavins or essential oils out of the eluent, for example,
cannabidiol (CBD), cannabinol (CBN), cannabigerol (CBG),
cannabichromene (CBC), cannabidivarol (CBDV), tetrahydrocannabidiol
(THCBD), tetrahydrocannabigerol (THCBG), tetrahydrocannabichromene
(THCBC), tetrahydrocannabidivarol (THCBDV), .DELTA..sup.8-THC, the
carboxylic acid precursors of the foregoing compounds, and related
naturally occurring compounds and their derivatives. In alternate
embodiments, the chromatographic column is arranged for
fractionating (e.g., sequentially fractionating) cannflavins and
related naturally occurring compounds and their derivatives. In
alternate embodiments, the system is arranged to fractionate the
components of essential oils. The list of compounds provided herein
is not exhaustive and is in no way intended to be limiting. In
these embodiments, the compound(s) of interest are retained or
detained (e.g., reversibly bound) on the column so that fractions
(alternatively, the last fractions) of the extract eluted from the
column contain the compounds(s) of interest. In alternative
embodiments, fractions containing the compound(s) of interest are
pooled. In some embodiments, different compounds may be extracted
with different solvents and then combined into a single extract. As
will be appreciated by one knowledgeable in the art, in this
manner, several different cannabinoids could be purified from a
single extract.
[0493] In alternative embodiments, the "plant material or
microbial" will be derived from one or more cannabis or hemp
plants, or from other plants, or microbes such as yeast, lichen,
algae or bacteria. The term "plant or microbial material"
encompasses a plant or plant part (e.g. bark, wood, leaves, stems,
roots, flowers, fruits, seeds, berries or parts thereof) as well as
exudates, and includes material falling within the definition of
"botanical raw material" in the Guidance for Industry Botanical
Drug Products Draft Guidance, August 2000, US Department of Health
and Human Services, Food and Drug Administration Centre for Drug
Evaluation and Research. The term "cannabis plant(s)" encompasses
wild type Cannabis sativa and also variants thereof, including
Cannabis chemovars (varieties characterized by virtue of chemical
composition) which naturally contain different amounts of the
individual cannabinoids, also Cannabis sativa subspecies indica
including the variants var. indica and var. kafiristanica, Cannabis
indica and also plants which are the result of genetic crosses,
self-crosses or hybrids thereof. The term "Cannabis plant material"
also can encompass plant material derived from one or more cannabis
plants, and can comprise any "cannabis plant material" including,
e.g., herbal Cannabis and dried Cannabis biomass. The term
"Cannabis plant material" also can encompass "decarboxylated
Cannabis plant material", which refers to Cannabis plant material
which has been subject to a decarboxylation step in order to
convert cannabinoid acids to the corresponding free
cannabinoids.
[0494] In alternative embodiments, a starting material for a
purification process as provided herein is an extract solution
containing a cannabinoid or cannabinoid acid obtained from a
natural or a synthetic source, e.g., a plant or microbial material.
In alternative embodiments, the "extract solution containing a
cannabinoid or cannabinoid acid" comprises a solvent extract of a
plant or microbial material. Solvents used for extraction for use
in the preparation of extract solutions can comprise non-polar
solvents, polar solvents such as ethanol, methanol or water, or
organic solvents such as liquid carbon dioxide, and combinations
thereof. The solvent may be an organic solvent, selected from the
group consisting of: non-polar solvents include liquid non-polar
solvents comprising lower C1-C12, preferably C3 to C8, straight
chain or branched chain alkanes for example, methane, ethane,
propane, butane, pentane, toluene, trimethylpentane; a low
molecular weight alcohol, polar solvents consisting of for example,
ethanol, methanol, water; a low molecular weight chlorinated
hydrocarbon, for example, chloroform and dichloromethane; or a
supercritical fluid such as CO.sub.2 with or without an organic
solvent modifier.
[0495] In alternative embodiments, the extract is prepared by
dissolving or partially dissolving the natural or synthetic, or the
plant or microbial material in a solvent, removing insoluble
material from the resultant solution (optionally by filtration with
or without activated carbon, precipitation, centrifugation and the
like), and optionally removing some or all of the extraction
solvent from the solution (optionally by rotary evaporation) to
form an extract or extract solution or concentrate containing a
cannabinoid or cannabinoid acid.
[0496] In alternative embodiments, extractions can comprise using a
technique referred to as accelerated solvent extraction or may use
subcritical water or any combination of water and solvent. In one
embodiment, when isolating cannabinoid acids, a modified pH
gradient elution solution is used. The primary purpose of this pH
adjustment (the modified pH gradient) is to promote or prevent
ionization of the cannabinoid acid. pH modified gradient elution
solutions may be achieved by the additional of a small volume of
acid or base to the solvent. It may be sufficient to add a
relatively weak acid, such as acetic acid, oxalic acid, glycolic
acid, carbonic acid or ammonium hydroxide or a small amount of base
or buffering agent such as sodium hydroxide, magnesium hydroxide,
sodium carbonate or sodium bicarbonate. For any given purification
process the optimal amount and type of acid or base used may be
determined empirically. An alternative exemplary acidified solvent
is 0.1% acetic acid in ethanol or 0.1% sodium hydroxide in ethanol.
In alternative embodiments, the neutralizing agent consists of for
example sodium hydroxide, sodium carbonate, potassium carbonate,
and potassium t-amylate, sodium bicarbonate.
[0497] Acidified non-polar and polar solvents of the types
described above can be useful in preparation of gradient elutions
using ion exchange chromatography. The solvents used in the
conversion can comprise an organic solvent, e.g., a non-polar
solvent, including a liquid non-polar solvent comprising lower
C1-C12, or C3 to C8, straight chain or branched chain alkanes for
example, methane, ethane, propane, butane, pentane, toluene,
trimethylpentane, hexane; a low molecular weight alcohol, polar
solvents consisting of for example, ethanol; a low molecular weight
chlorinated hydrocarbon, for example, chloroform and
dichloromethane; a heterocyclic compound or cyclic ether for
example, tetrahydrofuran and 2-Methyltetrahydrofuran and aromatic
ring hydrocarbons such as benzene, toluene, xylene and
ethylbenzene.
[0498] In alternative embodiments, the plant or microbial material
is subjected to a decarboxylation step prior to solvent extraction.
The purpose of the decarboxylation step is to convert cannabinoid
acids present in the plant or microbial material to the
corresponding free cannabinoids. In alternative embodiments, the
decarboxylation is carried out by heating the plant or microbial
material to a defined temperature for a suitable length of time.
Decarboxylation of cannabinoid acids is a function of time and
temperature, thus at higher temperatures a shorter period of time
will be taken for complete decarboxylation of a given amount of
cannabinoid acid. In alternative embodiments selecting appropriate
conditions for decarboxylation consideration includes minimizing
thermal degradation of the desirable, pharmacological cannabinoids
into undesirable degradation products, particularly thermal
degradation of .DELTA..sup.9-THC. In alternative embodiments, the
decarboxylation is carried out in a multi-step heating process in
which the plant or microbial material is: i) heated to a first
temperature for a first (relatively short) time period to evaporate
off retained water and allow for uniform heating of the plant or
microbial material; and ii) the temperature is increased to a
second temperature for a second time period (typically longer than
the first time period) until at least 95% conversion of the acid
cannabinoids to their neutral form has occurred.
[0499] In alternative embodiments, the "extract containing a
cannabinoid or a cannabinoid acid" prepared from the starting plant
or microbial material comprises a "botanical drug substance"
prepared from the plant material, or a polar or non-polar solvent
solution of such a botanical drug substance. In the context of this
application a "botanical drug substance" is an extract derived from
plant or microbial material, which extract fulfills the definition
of "botanical drug substance" provided in the Guidance for Industry
Botanical Drug Products Draft Guidance, August 2000, US Department
of Health and Human Services, Food and Drug Administration Centre
for Drug Evaluation and Research of: "A drug substance derived from
one or more plants, algae, or macroscopic fungi. It is prepared
from botanical raw materials by one or more of the following
processes: pulverization, decoction, expression, aqueous
extraction, ethanolic extraction, or other similar processes."
"Botanical drug substances" derived from cannabis plants include
primary extracts prepared by such processes as, for example,
maceration, percolation, and solvent extraction.
[0500] In alternative embodiments, solvent extraction may be
carried out using essentially any solvent that dissolves, or
substantially dissolves, cannabinoids/cannabinoid acids, such as
for example C1 to C5 alcohols (e.g. ethanol, methanol), C5-C12
alkanes (e.g. hexane), norflurane (HFA134a),
1,1,1,2,3,3,3-Heptafluoropropane (or HFA227), chloroform,
dichloromethane, dichloroethane and carbon dioxide. When solvents
such as those listed above are used, the resultant extract
typically contains non-specific lipid-soluble material. This can
optionally be removed by a variety of processes including
filtration to remove solids, "winterization", which involves for
example chilling to -20.degree. C. or lower followed by filtration
to remove waxy ballast, extraction with liquid carbon dioxide and
by distillation.
[0501] In alternative embodiments, any protocol for the preparation
of botanical drug substances from cannabis and hemp plant material
can be used, e.g., as described in International patent application
WO 02/064109. In alternative embodiments, the botanical drug
substance is obtained by carbon dioxide (CO.sub.2) extraction,
polar solvent extraction or non-polar solvent extraction or
combinations thereof followed by a filtration. Optionally a
secondary extraction is performed to remove a substantial
proportion of non-cannabinoid materials, e.g. waxes, wax esters and
glycerides, unsaturated fatty acid residues, terpenes, carotenes,
and flavonoids and other ballast.
[0502] In alternative embodiments, if it is intended to prepare
free cannabinoids from the plant or microbial material, e.g.,
cannabis, then the material is heated to a defined temperature for
a defined period of time in order to partially or substantially
decarboxylate cannabinoid acids to free cannabinoids prior to
extraction of the botanical drug substance. In alternative
embodiments, the botanical drug substance is prepared according to
a process comprising the following steps: i) optional
decarboxylation of the plant or microbial material, ii) extraction
with polar or non-polar solvent, to produce a crude botanical drug
substance, iii) optional precipitation with C1-C5 alcohol to reduce
the proportion of non-target materials, iv) removal of the
precipitate (preferably by filtration), v) optional treatment with
activated charcoal, and vi) evaporation to remove C1-C5 alcohol and
water, thereby producing a final botanical drug substance.
[0503] In alternative embodiments, provided are methods for
converting first substantially purified cannabidiol (CBD) to
.DELTA.8-tetrahydrocannabinol (.DELTA.8-THC) and
.DELTA.9-tetrahydrocannabinol (.DELTA.9-THC), and then further
converting the produced .DELTA.9-tetrahydrocannabinol
(.DELTA.9-THC) to .DELTA.8-tetrahydrocannabinol (.DELTA.8THC). As
will be appreciated by one knowledgeable in the art and as
discussed below, the reaction times may be varied somewhat,
producing product at different yields and purities. Furthermore,
functional equivalents may be substituted where appropriate.
[0504] In alternative embodiments, an exemplary method of
converting CBD to .DELTA.8-tetrahydrocannabinol (.DELTA.8-THC) and
.DELTA.9-tetrahydrocannabinol (.DELTA.9-THC) and further converting
(.DELTA.9-THC) to (.DELTA.8-THC) comprises: providing a reaction
mixture comprising a Lewis acid or non-oxidizing acid catalyst in a
reaction solvent, adding a substantially pure CBD or substantially
pure CBD and/or (.DELTA.9-THC) to the reaction mixture, mixing said
reaction mixture for a period of time; adding a neutralizing agent
to said mixture when it is observed that
.DELTA..sup.8-tetrahydrocannabinol (.DELTA.8-THC) is substantially
formed and .DELTA.9-tetrahydrocannabinol (.DELTA.9-THC) has been
substantially eliminated; filtration of catalyst and neutralizing
agent from mixture; optionally allowing mixture to separate into an
aqueous and organic phase; optionally adding a further solvent;
adding organic phase to a continuous or fixed bed chromatography
column and eluting the .DELTA.8-tetrahydrocannabinol (.DELTA.8-THC)
from the organic phase. The tetrahydrocannabinol may then be
combined with suitable excipients known in the art, thereby forming
a pharmaceutical composition. In one embodiment, the mixture is
allowed to separate into an aqueous phase and an organic phase; and
optionally the process further comprises removing the organic
phase).
[0505] In alternative embodiments, the tetrahydrocannabinol at
therapeutically effective concentrations or dosages is combined
with a pharmaceutically or pharmacologically acceptable carrier,
excipient or diluent, either biodegradable or non-biodegradable.
Exemplary examples of carriers include, but are by no means limited
to, for example, poly(ethylene-vinyl acetate), copolymers of lactic
acid and glycolic acid, poly(lactic acid), gelatin, collagen
matrices, polysaccharides, poly(D,L lactide), poly(malic acid),
poly(caprolactone), celluloses, albumin, starch, casein, dextran,
polyesters, ethanol, methacrylate, polyurethane, polyethylene,
vinyl polymers, glycols, mixtures thereof and the like. Standard
excipients that can be used include gelatin, casein, lecithin, gum
acacia, cholesterol, tragacanth, stearic acid, benzalkoniurn
chloride, cal ciurn stearate, glyceryl monostearate, cetostearyl
alcohol, cetomacrogol emulsifying wax, sorbitan esters,
polyoxyethylene alkyl ethers, polyoxyethylene castor oil
derivatives, polyoxyethylene sorbitan fatty acid esters,
polyethylene glycols, polyoxyethylene stearates, colloidol silicon
dioxide, phosphates, sodium dodecyl sulfate, carboxymethylcellulose
calcium, carboxymethylcellulose sodium, methylcellulose,
hydroxyethylcellulose, hydroxypropylcellulose,
hydroxypropylmethycellulose phthalate, non-crystalline cellulose,
magnesium aluminum silicate, triethanolamine, polyvinyl alcohol,
polyvinylpyrrolidone, sugars and starches. See, for example,
Remington: The Science and Practice of Pharmacy, 1995, Gennaro
ed.
[0506] In some embodiments, the catalyst is a Lewis acid, for
example, p-toluenesulfonic acid, boron trifluoride or
BF.sub.3Et.sub.2O. In some embodiments, the BF.sub.3Et.sub.2O
(boron trifluoride diethyl etherate) is in dry methylene chloride,
ethyl acetate, ethanol, hexane or other organic solvent. In yet
other examples, the catalyst may be hydrochloric acid in ethanol or
sulfuric acid in cyclohexane.
[0507] In some embodiments, the catalyst is a non-oxidizing acid,
for example, formic acid, acetic acid or hydrobromic acid. In some
embodiments, the non-oxidizing acid is in dry methylene chloride,
ethyl acetate, ethanol, hexane or other organic solvent.
[0508] In some embodiments, a base is added to the reaction mixture
prior to allowing the reaction mixture to separate into organic and
aqueous phases. The base may be an alkali metal hydrogen carbonate,
carbonate of an alkali metal, lithium hydroxide (LiOH), sodium
hydroxide (NaOH), potassium hydroxide (KOH), rubidium hydroxide
(RbOH), cesium hydroxide (CsOH), magnesium hydroxide (Mg(OH)),
calcium hydroxide (Ca(OH)), strontium hydroxide (Sr(OH)), barium
hydroxide (Ba(OH)).
[0509] In some embodiments, the organic layer is dried prior to
eluting. In these embodiments, a suitable drying or dehydration
compound, for example, MgSO.sub.4 or Na.sub.2SO.sub.4 is used.
[0510] In yet other embodiments, the process may be carried out
under a nitrogen (e.g., N.sub.2) atmosphere.
[0511] In alternative embodiments, and as discussed below, yield is
determined by looking at the peak area for the isolated compound in
the gas chromatography-mass spectra analysis of the crude reaction
product mixture and the final reaction product mixture. It is
important to note that in the prior art, yield is often calculated
on the basis of first isolated crude product before final
purification. In some embodiments of processes provided herein
yield of .DELTA.8-THC is at least about 75%; in other embodiments,
the yield of .DELTA.8-THC is at least about 90%; and in other
embodiments, yield of .DELTA.8-THC is at least about 98%; and in
yet other embodiments, yield of .DELTA.8-THC is between about 75 to
98% or 99%.
[0512] In alternative embodiments, purity is determined by Gas
Chromatography-Mass Spectrometry (GC-MS) and/or by analytical
high-performance liquid chromatography (HPLC). The total ion
chromatogram from the GC-MS gives information similar to that
provided by a flame ionization detector (FID)-GC in that the peak
area is proportional to the mass of the analytes detected. Total
peak area and the peak areas of the individual analytes can be
compared in the GC-MS case as long as the masses are in generally
the same range. As discussed below, in some embodiments, purity of
the .DELTA.8-THC isolated by the process is greater than about 90%,
95%, 97% or 98%, or purity is greater that about 98% to 99%.
Chapter IV
[0513] In alternative embodiments, provided are continuous
isolation and purification processes for preparing a substantially
pure cannabinoids or a product enriched in cannabis from plant or
microbial material extracts. In alternative embodiments, provided
herein are improved methods for purification and conversion
processes based on a simple combination of continuous
chromato-graphic gradient elutions. This exemplary process is
simple, efficient and economic.
[0514] In alternative embodiments, provided are processes for
producing and isolating cannabinoids from cannabis and hemp
extracts which contain cannabinoids in minute amounts. In
alternative embodiments, provided are processes for producing and
isolating cannabinoids from natural materials, including plant or
plant extracts, microbes, or botanical drug substances, or
synthetically and semi-synthetically prepared cannabinoid products,
or from recombinantly engineered microbes, e.g., yeasts or bacteria
recombinantly engineered to express one or more cannabinoids. In
one embodiment, exemplary methods are inexpensive and provide
specific cannabinoid concentrates (e.g., of CBD, .DELTA..sup.8-THC,
.DELTA..sup.9-THC) of high purity.
[0515] In one embodiment, exemplary methods provide a simple and
economical continuous process for separating and concentrating
cannabinoids from solvent-extracted cannabinoid containing
materials. In alternative embodiments, the solvent-extracted
cannabinoid containing materials are derived from synthetic or
biological materials such as hemp and Cannabis or botanical drug
substances, or from microbial materials; and the solvent extraction
methods can be polar solvent extractions, nonpolar solvent
extractions, or the solvent extraction methods can comprise use of
super critical carbon dioxide or mixtures thereof. The solvent
extraction methods can extract cannabinoids substantially from the
synthetic or biological, e.g., plant, matter, along with other
plant matter comprising lipids, waxes, monoterpenes,
sesquiterpenes, hydrocarbons, alkaloids, flavonoids and
chlorophylls.
[0516] In alternative embodiments, methods provided herein comprise
subjecting cannabinoid containing solvent extract starting
materials to a number of chromatographic resins in various
contacting steps using various gradient elution solutions.
[0517] In alternative embodiments, the cannabinoids from which can
be fractionated and isolated using methods as provided herein, or
which can be produced in reactions as provided herein, or which the
solvent extracts are derived can be from, or can comprise:
.DELTA..sup.9tetrahydrocannabinol (.DELTA..sup.9 THC); cannabidiol
(CBD); cannabinol (CBN); cannabigerol (CBG); cannabichromene (CBC);
cannabidivarol (CBDV); tetrahydrocannabidiol (THCBD);
tetrahydrocannabigerol (THCBG), tetrahydrocannabichromene (THCBC);
tetrahydrocannabidivarol (THCBDV), or combinations thereof,
including carboxylic acid precursors of the foregoing compounds and
related naturally occurring compounds and their derivatives.
[0518] In alternative embodiments, provides are methods of
preparing or obtaining a substantially pure cannabinoid or a
product enriched in a given cannabinoid comprising. [0519] (i)
obtaining an extract or extract solution containing a cannabinoid
or a cannabinoid acid from a plant or microbial material; [0520]
(ii) filtering the extract of step (i) to remove solids and color
bodies; [0521] (iii) removing the extract solvent (as an extract
fraction); [0522] (v) continuously loading of an amount of extract
solution over a defined time increment over multiple stationary
phase resins columns such as a normal phase, reverse phase and/or
ion exchange chromatographic resin; [0523] (vi) continuously
eluting the extract solution using multiple defined gradient
elution solutions at specific time increments and volumes; [0524]
(vii) continuously collecting the gradient elution fractions;
[0525] (viii) removal of the gradient elution solution from the
produced fractions to generate a substantially purified extract
[0526] (ix) optionally loading of specific first gradient elution
fractions on a reverse phase, ion exchange or normal
chromatographic resin; [0527] (x) continuously eluting the first
gradient elution fractions with a second gradient elution solvent;
and, [0528] (xi) removing of the second gradient elution solvent
from the produced fractions to produce a purified extract.
[0529] In alternative embodiments, the methods further
comprise:
[0530] a step (xii): loading of first and second gradient elution
solvents s onto an ion exchange chromatographic resin;
[0531] a step (xiii): eluting an extract solution from the ion
exchange chromatographic resin of step (xi) using a gradient
solvent solution and collecting the gradient elution fractions;
and
[0532] (xiv) removing the gradient elution solution from the
produced fractions of step (xiii) to produce purified and
substantially purified extract.
[0533] In alternative embodiments, a "substantially pure"
preparation of cannabinoid is defined as a preparation having a
chromatographic purity (of the desired cannabinoid or cannabinoid
acid) of greater than about 75%, or greater than about 96%, or
greater than about 97%, or greater than about 98%, or greater than
about 99%, or greater than about 99.5%, or between about 70% and
99.9%, as determined by area normalization of an HPLC profile.
[0534] In alternative embodiments, the term "product enriched in a
given cannabinoid" encompasses preparations having at least about
50%, or greater than about 75%, or greater than about 90%, 95% or
98%, or between about 50% and 99.9%, chromatographic purity, for
the desired cannabinoid.
[0535] In alternative embodiments, a non-purified, or
non-substantially purified, product can comprise a greater
proportion of impurities, non-target materials and/or other
cannabinoids than a "substantially pure" preparation. The
cannabinoid can be (e.g., a cannabinoid purified or isolated by, or
made by a reaction of, a method as provided herein can be):
.DELTA..sup.9tetrahydrocannabinol (.DELTA..sup.9 THC); cannabidiol
(CBD); cannabinol (CBN); cannabigerol (CBG); cannabichromene (CBC);
cannabidivarol (CBDV); tetra-hydrocannabidiol (THCBD);
tetra-hydrocannabigerol (THCBG); tetra-hydrocannabichromene
(THCBC); or, tetra-hydrocannabidivarol (THCBDV); the carboxylic
acid precursors of the foregoing compounds; and related naturally
occurring compounds and their derivatives.
[0536] In alternative embodiments, the term "cannabinoids" e.g., a
cannabinoid purified or isolated by, or made by a reaction of a
process as provided herein, includes or refers to a family of
natural products that can contain a 1,1'-di-methyl-pyrane ring, a
variedly derivatized aromatic ring and/or a variedly unsaturated
cyclohexyl ring and their immediate chemical precursors.
[0537] In alternative embodiments, the term "cannflavins", e.g., a
cannflavin purified or isolated by, or made by a reaction of a
process as provided herein, includes or refers to a family of
natural products that can contain a 1,4-pyrone ring fused to a
variedly derivatized aromatic ring and linked to a second variedly
derivatized aromatic ring.
[0538] In alternative embodiments, the term "essential oils", e.g.,
an essential oil used as a starting material in a process as
provided herein, or an essential oil that may be isolated by a
process as provided herein, includes or refers to a family of
natural products that can contain a multiple of the 5-membered
isoprene unit variedly substituted, often cyclized to form one or
more ring systems; they may also contain series of aldehydes and/or
ketones and esters of a variety of carboxylic acid substituted
compounds.
[0539] In alternative embodiments, provided are methods for
extracting and/or purifying cannabinoids from any plant or
microbial material extract known to contain such cannabinoids,
cannflavins and essential oils; and, optionally to purify
cannflavins and to optionally purify essential oils. In alternative
embodiments, the extract is passed through a series of
chromatographic columns, for example, a normal phase column, a
reversed phase column or an ion exchange column as a continuous
simulated moving bed configuration.
[0540] In one embodiment, the chromatographic column is arranged
for gradient elution fractioning using normal phase, reverse phase
and/or ion exchange chromatography. In one embodiment
.DELTA..sup.9-THC and CBD are fractionated out of the eluent. For
example, in one embodiment, as the extract is passed over the
column, .DELTA..sup.9-THC and CBD is differentially retained or
detained (e.g., reversibly bound) on the column. As a result, as
the extract comes off the column, the initial fractions eluted off
the column will be (substantially) free of .DELTA..sup.9-THC and
CBD. The fractions free of .DELTA..sup.9-THC and CBD are pooled,
thereby producing an extract with .DELTA..sup.9-THC and CBD
substantially removed (e.g., in alternative embodiments,
"substantially removed" or "substantially fractionated" means at
least 85%, 90%, 95%, 98%, 99% or 99.5% or more removed or
fractionated).
[0541] In some embodiments, (substantially) only .DELTA..sup.9-THC
is substantially fractionated out of the eluent. For example, in
one embodiment, as the extract or pooled fractions from a previous
normal phase, reverse phase and/or ion exchange elution are passed
over the column, .DELTA..sup.9-THC is differentially retained or
detained (e.g., reversibly bound) on the column. As a result, as
the extracts are pooled previous elution fractions comes off the
column, the initial fractions eluted off the column will be free of
.DELTA..sup.9-THC. These fractions free of .DELTA..sup.9-THC are
pooled, thereby producing an extract with .DELTA..sup.9-THC
substantially removed (e.g., in alternative embodiments,
"substantially removed" or "substantially fractionated" means at
least 85%, 90%, 95%, 98%, 99% or 99.5% or more removed or
fractionated).
[0542] In some embodiments, when using a continuous chromatography
apparatus or device, a series of columns are arranged for example
3, 4, 5, 6, 7, 8 or more, or between 3 and 30 or more, columns are
arranged in a continuous rotation traveling through a series of
contact points where gradient elution solutions and extract
solution are introduced at fixed points for a period of time
allowing for continuous loading and elution, and collection of
fractions. The first column is loaded with extract solution at the
first position (or station). The first column is then moved to the
second position where the first gradient elution is introduced (or
loaded) while at the same time the second column is loaded with
extract solution at position one. The first column then rotates
(i.e., is moved) to the third position where the second gradient
solvent is introduced, the second column moves to the second
position where the first gradient solvent is introduced and the
third column is loaded with extract solution at position one. The
first column then moves to the fourth position where the third
gradient solvent is introduced, the second column moves to the
third position where the second gradient solvent is introduced, the
third column moves to the second position where the first gradient
solvent is introduced and the fourth column is loaded with extract
solution at position one. The first column then moves to the fifth
position where the fourth gradient solvent is introduced, the
second column moves to the fourth position where the third gradient
solvent is introduced, the third column moves to the third position
where the second gradient is introduced, the fourth column moves to
the second position where the first gradient solvent is introduced
and the fifth column is loaded with extract solution at position
one. The first column then moves to the sixth position where the
fifth gradient solvent is introduced, the second column moves to
the fifth position where the fourth gradient is introduced, the
third column moves to the fourth position where the third gradient
solvent is introduced, the fourth column moves to the third
position where the second gradient solvent is introduced, the fifth
column moves to the second position where the first gradient
solvent is introduced and the sixth column is loaded with extract
solution at position one. The first column then moves or returns to
the first position where extract solution is loaded, the second
column moves to the sixth position where the fifth gradient solvent
is introduced, the third column moves to the fifth position where
the fourth gradient solvent is introduced, the fourth column moves
to the fourth position where the third gradient solvent is
introduced, the fifth column moves to the third position where the
second gradient solvent is introduced and the sixth column moves to
the second position where the first gradient solvent is introduced.
The equipment is generally described in U.S. Pat. Nos. 4,764,276,
4,808,317, and 4,710,364, for example, each of which are expressly
incorporated herein by reference.
[0543] In a particular embodiment the first gradient solvent elutes
CBD, CBG and CBN and is substantially fractionated in the eluent.
For example, in one embodiment, as the extract or pooled fractions
from a normal phase, reverse phase and/or ion exchange elution are
passed over the column, CBD, CBG and CBN is differentially produced
in the eluent (e.g., by use of a gradient elution process). In a
particular embodiment second gradient solvent elutes CBD and is
substantially fractionated in the eluent. For example, in one
embodiment, as the extract or pooled fractions from a normal phase,
reverse phase and/or ion exchange elution are passed over the
column, CBD is differentially produced in the eluent. In a
particular embodiment the third gradient solvent elutes CBD and
.DELTA..sup.9-THC and is substantially fractionated in the eluent.
For example, in one embodiment, as the extract or pooled fractions
from a normal phase, reverse phase and/or ion exchange elution are
passed over the column, CBD and .DELTA..sup.9-THC is differentially
produced in the eluent. In a particular embodiment the fourth
gradient elutes .DELTA..sup.9-THC and is substantially fractionated
in the eluent. For example, in one embodiment, as the extract or
pooled fractions from a normal phase, reverse phase and/or ion
exchange elution are passed over the column, .DELTA..sup.9-THC is
differentially produced in the eluent. In a particular embodiment
the fifth gradient elutes CBC, THC-A, terpenes and
.DELTA..sup.9-THC and is substantially fractionated in the eluent.
For example, in one embodiment, as the extract or pooled fractions
from a normal phase, reverse phase and/or ion exchange elution are
passed over the column, CBC, THC-A, terpenes and .DELTA..sup.9-THC
is differentially produced in the eluent.
[0544] In some embodiments the fractions each produced fraction
from the first continuous gradient elution separation is collected
and the elution solution is removed. The collected fraction of
cannabinoids is then loaded onto a normal phase, reverse phase or
ion exchange resin and subject to the before mentioned continuous
chromatography method to either preferentially purify a specific
target cannabinoid from minor cannabinoids contained in the
collected fraction.
[0545] In some embodiments, (substantially) only CBD is
substantially fractionated out of the eluent. For example, in one
embodiment, as the extract or pooled fractions from a previous
normal phase, reverse phase and/or ion exchange elution are passed
over the column, CBD is differentially retained or detained (e.g.,
reversibly bound) on the column. As a result, as the extract or
pooled previous elution fractions comes off the column, the initial
fractions eluted off the column will be (substantially) free of
CBD. These fractions free of CBD are pooled, thereby producing an
extract with CBD substantially removed (e.g., in alternative
embodiments, "substantially removed" or "substantially
fractionated" means at least 85%, 90%, 95%, 98%, 99% or 99.5% or
more removed or fractionated).
[0546] In some embodiments, .DELTA..sup.9-THC carboxylic acid
species or .DELTA..sup.9-THCA and CBD carboxylic acid species CBDA
are fractionated out of out of the eluent. For example, in one
embodiment, as the extract or pooled fractions from a first elution
are passed over the column, .DELTA..sup.9-THCA and CBDA are
differentially retained or detained (e.g., reversibly bound) on the
column. As a result, as the extract or pooled first elution
fractions comes off the column, the initial fractions eluted off
the column will be (substantially) free of .DELTA..sup.9-THCA and
CBDA. These fractions (substantially) free of .DELTA..sup.9-THCA
and CBDA are pooled, thereby producing an extract with
.DELTA..sup.9-THCA and CBDA substantially removed (e.g., in
alternative embodiments, "substantially removed" or "substantially
fractionated" means at least 85%, 90%, 95%, 98%, 99% or 99.5% or
more removed or fractionated).
[0547] In some embodiments, the .DELTA..sup.9-THC may be eluted
from the column, extracted or concentrated, for purifying, or
substantially purifying, .DELTA..sup.9-THC. In alternative
embodiments, the chromatographic column is arranged for continuous
fractionation of a specific cannabinoid or groups of cannabinoids
or their carboxylic acid species, cannflavin or essential oil or
class of cannabinoids, cannflavins or essential oils out of the
eluent, for example, cannabidiol (CBD), cannabinol (CBN),
cannabigerol (CBG), cannabichromene (CBC), cannabidivarol (CBDV),
tetrahydrocannabidiol (THCBD), tetrahydrocannabigerol (THCBG),
tetrahydrocannabichromene (THCBC), tetrahydrocannabidivarol
(THCBDV), .DELTA..sup.8-THC, the carboxylic acid precursors of the
foregoing compounds, and related naturally occurring compounds and
their derivatives. In alternate embodiments, the chromatographic
column is arranged for continuous fractionation cannflavins and
related naturally occurring compounds and their derivatives. In
alternate embodiments, the system is arranged to continuously
fractionate the components of essential oils. The list of compounds
provided herein is not exhaustive and is in no way intended to be
limiting. In these embodiments, the compound(s) of interest are
retained or detained (e.g., reversibly bound) on the column so that
fractions (alternatively, the last fractions) of the extract eluted
from the column contain the compounds(s) of interest. In
alternative embodiments, fractions containing the compound(s) of
interest are pooled. In some embodiments, different compounds may
be extracted with different solvents and then combined into a
single extract. As will be appreciated by one knowledgeable in the
art, in this manner, several different cannabinoids could be
purified from a single extract.
[0548] In alternative embodiments, the "plant material or
microbial" will be derived from one or more cannabis or hemp
plants, or from other plants, or from a microbial species,
including fungi, lichen, yeast and bacteria. The term "plant
material" encompasses a plant or plant part (e.g. bark, wood,
leaves, stems, roots, flowers, fruits, seeds, berries or parts
thereof) as well as exudates, and includes material falling within
the definition of "botanical raw material" in the Guidance for
Industry Botanical Drug Products Draft Guidance, August 2000, US
Department of Health and Human Services, Food and Drug
Administration Centre for Drug Evaluation and Research. The term
"cannabis plant(s)" encompasses wild type Cannabis sativa and also
variants thereof, including Cannabis chemovars (varieties
characterized by virtue of chemical composition) which naturally
contain different amounts of the individual cannabinoids, also
Cannabis sativa subspecies indica including the variants var.
indica and var. kafiristanica, Cannabis indica and also plants
which are the result of genetic crosses, self-crosses or hybrids
thereof. The term "Cannabis plant material" also can encompass
plant material derived from one or more cannabis plants, and can
comprise any "cannabis plant material" including, e.g., herbal
Cannabis and dried Cannabis biomass. The term "Cannabis plant
material" also can encompass "decarboxylated Cannabis plant
material", which refers to Cannabis plant material which has been
subject to a decarboxylation step in order to convert cannabinoid
acids to the corresponding free cannabinoids.
[0549] In alternative embodiments, a starting material for a
purification process as provided herein is an extract solution
containing a cannabinoid or cannabinoid acid obtained from a
natural or a synthetic source, e.g., a plant or microbial material.
In alternative embodiments, the "extract solution containing a
cannabinoid or cannabinoid acid" comprises a solvent extract of a
plant or microbial material. Solvents used for extraction for use
in the preparation of extract solutions can comprise non-polar
solvents, polar solvents such as ethanol, methanol or water, or
organic solvents such as liquid carbon dioxide, and combinations
thereof. The solvent may be an organic solvent, selected from the
group consisting of: non-polar solvents include liquid non-polar
solvents comprising lower C1-C12, for example, C3 to C8, or
straight chain or branched chain alkanes for example, methane,
ethane, propane, butane, pentane, toluene, trimethylpentane; a low
molecular weight alcohol, polar solvents consisting of for example,
ethanol, methanol, water; a low molecular weight chlorinated
hydrocarbon, for example, chloroform and dichloromethane; or a
supercritical fluid such as CO.sub.2 with or without an organic
solvent modifier.
[0550] In alternative embodiments, the extract is prepared by
dissolving or partially dissolving the natural or synthetic, or the
plant or microbial material in a solvent, removing insoluble
material from the resultant solution (optionally by filtration with
or without activated carbon, precipitation, centrifugation and the
like), and optionally removing some or all of the extraction
solvent from the solution (optionally by rotary evaporation) to
form an extract or extract solution or concentrate containing a
cannabinoid or cannabinoid acid.
[0551] In alternative embodiments, extractions can comprise using a
technique referred to as accelerated solvent extraction or may use
subcritical water or any combination of water and solvent. In one
embodiment, when isolating cannabinoid acids, a modified pH
gradient elution solution is used. The primary purpose of this pH
adjustment (the modified pH gradient) is to promote or prevent
ionization of the cannabinoid acid. pH modified gradient elution
solutions may be achieved by the additional of a small volume of
acid or base to the solvent. It may be sufficient to add a
relatively weak acid, such as acetic acid, oxalic acid, glycolic
acid, carbonic acid or ammonium hydroxide or a small amount of base
or buffering agent such as sodium hydroxide, magnesium hydroxide,
sodium carbonate or sodium bicarbonate. For any given purification
process the optimal amount and type of acid or base used may be
determined empirically. An alternative exemplary acidified solvent
is 0.1% acetic acid in ethanol or 0.1% sodium hydroxide in ethanol.
In alternative embodiments, the neutralizing agent consists of for
example sodium hydroxide, sodium carbonate, potassium carbonate,
and potassium t-amylate, sodium bicarbonate.
[0552] Acidified non-polar and polar solvents of the types
described above can be useful in preparation of gradient elutions
using ion exchange chromatography. The solvents used in the
conversion can comprise an organic solvent, e.g., a non-polar
solvent, including a liquid non-polar solvent comprising lower
C1-C12, or C3 to C8, straight chain or branched chain alkanes for
example, methane, ethane, propane, butane, pentane, toluene,
trimethylpentane, hexane; a low molecular weight alcohol, polar
solvents consisting of for example, ethanol; a low molecular weight
chlorinated hydrocarbon, for example, chloroform and
dichloromethane; a heterocyclic compound or cyclic ether for
example, tetrahydrofuran and 2-Methyltetrahydrofuran and aromatic
ring hydrocarbons such as benzene, toluene, xylene and
ethylbenzene.
[0553] In alternative embodiments, the plant or microbial material
is subjected to a decarboxylation step prior to solvent extraction.
The purpose of the decarboxylation step is to convert cannabinoid
acids present in the plant or microbial material to the
corresponding free cannabinoids. In alternative embodiments, the
decarboxylation is carried out by heating the plant or microbial
material to a defined temperature for a suitable length of time.
Decarboxylation of cannabinoid acids is a function of time and
temperature, thus at higher temperatures a shorter period of time
will be taken for complete decarboxylation of a given amount of
cannabinoid acid. In alternative embodiments selecting appropriate
conditions for decarboxylation consideration include minimizing
thermal degradation of the desirable, pharmacological cannabinoids
into undesirable degradation products, particularly thermal
degradation of .DELTA..sup.9-THC. In alternative embodiments, the
decarboxylation is carried out in a multi-step heating process in
which the plant or microbial material is: i) heated to a first
temperature for a first (relatively short) time period to evaporate
off retained water and allow for uniform heating of the plant or
microbial material; and ii) the temperature is increased to a
second temperature for a second time period (typically longer than
the first time period) until at least 95% conversion of the acid
cannabinoids to their neutral form has occurred.
[0554] In alternative embodiments, the "extract containing a
cannabinoid or a cannabinoid acid" prepared from the starting plant
or microbial material comprises a "botanical drug substance"
prepared from the plant or microbial material, or a polar or
non-polar solvent solution of such a botanical drug substance. In
the context of this application a "botanical drug substance" is an
extract derived from plant or microbial material, which extract
fulfills the definition of "botanical drug substance" provided in
the Guidance for Industry Botanical Drug Products Draft Guidance,
August 2000, US Department of Health and Human Services, Food and
Drug Administration Centre for Drug Evaluation and Research of: "A
drug substance derived from one or more plants, algae, or
macroscopic fungi. It is prepared from botanical raw materials by
one or more of the following processes: pulverization, decoction,
expression, aqueous extraction, ethanolic extraction, or other
similar processes." "Botanical drug substances" derived from
cannabis plants include primary extracts prepared by such processes
as, for example, maceration, percolation, and solvent
extraction.
[0555] In alternative embodiments, solvent extraction may be
carried out using essentially any solvent that dissolves, or
substantially dissolves, cannabinoids/cannabinoid acids, such as
for example C1 to C5 alcohols (e.g. ethanol, methanol), C5-C12
alkanes (e.g. hexane), norflurane, or 1,1,1,2-tetrafluoroethane
(fIFA134a), 1,1,1,2,3,3,3-Heptafluoropropane (or HFA227),
chloroform, dichloromethane, dichloroethane and carbon dioxide.
When solvents such as those listed above are used, the resultant
extract typically contains non-specific lipid-soluble material.
This can optionally be removed by a variety of processes including
filtration to remove solids, "winterization", which involves for
example chilling to -20.degree. C. or lower followed by filtration
to remove waxy ballast, extraction with liquid carbon dioxide and
by distillation.
[0556] In alternative embodiments, any protocol for the preparation
of botanical drug substances from cannabis and hemp plant material
can be used, e.g., as described in International patent application
WO 02/064109. In alternative embodiments, the botanical drug
substance is obtained by carbon dioxide (CO.sub.2) extraction,
polar solvent extraction or non-polar solvent extraction or
combinations thereof followed by a filtration. Optionally a
secondary extraction is performed to remove a substantial
proportion of non-cannabinoid materials, e.g. waxes, wax esters and
glycerides, unsaturated fatty acid residues, terpenes, carotenes,
and flavonoids and other ballast.
[0557] In alternative embodiments, if it is intended to prepare
free cannabinoids from the plant or microbial material (e.g.,
cannabis), then the material is heated to a defined temperature for
a defined period of time in order to partially or substantially
decarboxylate cannabinoid acids to free cannabinoids prior to
extraction of the botanical drug substance. In alternative
embodiments, the botanical drug substance is prepared according to
a process comprising the following steps: i) optional
decarboxylation of the plant material, ii) extraction with polar or
non-polar solvent, to produce a crude botanical drug substance,
iii) optional precipitation with C1-C5 alcohol to reduce the
proportion of non-target materials, iv) removal of the precipitate
(for example, by filtration, precipitation, centrifugation and the
like), v) optional treatment with activated charcoal, and vi)
evaporation to remove C1-C5 alcohol and water, thereby producing a
final botanical drug substance.
[0558] In alternative embodiments, the tetrahydrocannabinol at
therapeutically effective concentrations or dosages is combined
with a pharmaceutically or pharmacologically acceptable carrier,
excipient or diluent, either biodegradable or non-biodegradable.
Exemplary examples of carriers include, but are by no means limited
to, for example, poly(ethylene-vinyl acetate), copolymers of lactic
acid and glycolic acid, poly(lactic acid), gelatin, collagen
matrices, polysaccharides, poly(D,L lactide), poly(malic acid),
poly(caprolactone), celluloses, albumin, starch, casein, dextran,
polyesters, ethanol, methacrylate, polyurethane, polyethylene,
vinyl polymers, glycols, mixtures thereof and the like. Standard
excipients that can be used include gelatin, casein, lecithin, gum
acacia, cholesterol, tragacanth, stearic acid, benzalkonium
chloride, calcium stearate, glyceryl monostearate, cetostearyl
alcohol, cetomacrogol emulsifying wax, sorbitan esters,
polyoxyethylene alkyl ethers, polyoxyethylene castor oil
derivatives, polyoxyethylene sorbitan fatty acid esters,
polyethylene glycols, polyoxyethylene stearates, colloidol silicon
dioxide, phosphates, sodium dodecyl sulfate, carboxymethylcellulose
calcium, carboxymethyl cellulose sodium, methylcellulose,
hydroxyethylcellulose, hydroxypropylcellulose,
hydroxypropylmethycellulose phthalate, non-crystalline cellulose,
magnesium aluminum silicate, triethanolamine, polyvinyl alcohol,
polyvinylpyrrolidone, sugars and starches. See, for example,
Remington: The Science and Practice of Pharmacy, 1995, Gennaro
ed.
[0559] In alternative embodiments, purity is determined by Gas
Chromatography-Mass Spectrometry (GC-MS) and/or by analytical
high-performance liquid chromatography (HPLC). The total ion
chromatogram from the GC-MS gives information similar to that
provided by a flame ionization detector (FID)-GC in that the peak
area is proportional to the mass of the analytes detected. Total
peak area and the peak areas of the individual analytes can be
compared in the GC-MS case as long as the masses are in generally
the same range. As discussed below, in some embodiments, purity of
the isolated cannabinoids by the continuous process is greater than
about 90%, 95%, 97% or 98%, or purity is greater that about 98% to
99%.
Chapter V
[0560] In alternative embodiments, provided are continuous
isolation and purification processes for preparing a substantially
pure cannabidiol or a product enriched in cannabidiol from plant
material extracts. In alternative embodiments, provided herein are
improved methods for converting cannabidiol (CBD) to
.DELTA..sup.8-THC and .DELTA..sup.9-THC, including a purification
and conversion process based on a simple combination of continuous
chromato-graphic gradient elutions and semi continuous
isomerization reactions. This exemplary process is simple,
efficient and economic.
[0561] In alternative embodiments, provided are methods of
preparing cannabinoids in substantially pure form starting from
plant extract material and conversion of the purified CBD to form
both .DELTA..sup.8 THC and .DELTA..sup.9 THC and subsequent
purification of the produced .DELTA..sup.8 tetrahydrocannabinol
(.DELTA..sup.8 THC) into .DELTA..sup.9 tetrahydrocannabinol
(.DELTA..sup.9 THC) using continuous chromatography.
[0562] In alternative embodiments, provided are processes for
producing and isolating cannabinoids from cannabis and hemp
extracts which contain cannabinoids in minute amounts. In
alternative embodiments, provided are processes for producing and
isolating cannabinoids from natural materials, including plant or
plant extracts, microbes, or botanical drug substances, or
synthetically and semi-synthetically prepared cannabinoid products,
or from recombinantly engineered microbes, e.g., yeasts or bacteria
recombinantly engineered to express one or more cannabinoids. In
one embodiment, exemplary methods are inexpensive and provide
specific cannabinoid concentrates (e.g., of CBD, .DELTA..sup.8-THC,
.DELTA..sup.9-THC) of high purity.
[0563] In one embodiment, exemplary methods provide a simple and
economical continuous process for separating and concentrating
cannabinoids from solvent-extracted cannabinoid containing
materials. In one embodiment, exemplary methods provide a method
that first converts the substantially isolated CBD into a mixture
of .DELTA..sup.8-THC and .DELTA..sup.9-THC, and then subsequently
purifying or isolating the .DELTA..sup.8-THC and .DELTA..sup.9-THC.
In alternative embodiments, the solvent-extracted cannabinoid
containing materials are derived from synthetic or biological
materials such as hemp and Cannabis or botanical drug substances,
or from microbial materials; and the solvent extraction methods can
be polar solvent extractions, nonpolar solvent extractions, or the
solvent extraction methods can comprise use of super critical
carbon dioxide or mixtures thereof. The solvent extraction methods
can extract cannabinoids substantially from the synthetic or
biological, e.g., plant, matter, along with other plant matter
comprising lipids, waxes, monoterpenes, sesquiterpenes,
hydrocarbons, alkaloids, flavonoids and chlorophylls.
[0564] In alternative embodiments, methods provided herein comprise
subjecting cannabinoid containing solvent extract starting
materials to a number of chromatographic resins in various
contacting steps using various gradient elution solutions.
[0565] In alternative embodiments, the cannabinoids which can be
fractionated and isolated using methods as provided herein, or
which can be produced in reactions as provided herein, or from
which the solvent extracts are derived can be from, or can
comprise: .DELTA..sup.8tetrahydrocannabinol (.DELTA..sup.8 THC);
.DELTA..sup.9tetrahydrocannabinol (.DELTA..sup.9 THC); cannabidiol
(CBD); cannabinol (CBN); cannabigerol (CBG); cannabichromene (CBC);
cannabidivarol (CBDV); tetrahydrocannabidiol (THCBD);
tetrahydrocannabigerol (THCBG); tetrahydrocannabichromene (THCBC);
tetrahydrocannabidivarol (THCBDV), or combinations thereof,
including carboxylic acid precursors of the foregoing compounds and
related naturally occurring compounds and their derivatives.
[0566] In alternative embodiments, provides are methods of
preparing or obtaining a substantially pure cannabinoid or a
product enriched in a given cannabinoid comprising: [0567] (i)
obtaining or providing, or having provided, an extract or extract
solution, or an aliquot or sample, containing a cannabinoid or a
cannabinoid acid from a natural or the synthetic source, e.g., a
plant material; [0568] (ii) optionally filtering the extract or
extract solution, or aliquot or sample, of step (i) to remove
solids and color bodies; [0569] (iii) optionally removing the
extract solvent (as an extract fraction); [0570] (iv) continuously
loading of an amount of extract solution over a defined time
increment over multiple stationary phase resins columns such as a
normal phase, reverse phase and/or ion exchange chromatographic
resin; [0571] (v) continuously eluting the extract, or extract
solution, or aliquot or sample, solution using multiple defined
gradient elution solutions at specific time increments and volumes;
[0572] (vi) continuously collecting the gradient elution fractions;
[0573] (vii) removal of the gradient elution solution from the
produced fractions to generate a substantially purified extract, or
a substantially purified extract from each fraction; [0574] (viii)
optionally loading of specific first gradient elution fractions on
a reverse phase, ion exchange or normal chromatographic resin;
[0575] (ix) continuously eluting the first gradient elution
fractions with a second gradient elution solvent; and, [0576] (x)
removing of the second gradient elution solvent from the produced
fractions to produce a purified extract, or a substantially
purified extract from each fraction.
[0577] In alternative embodiments, the methods further
comprise:
[0578] a step (xi): loading of first and second gradient elution
solvents s onto an ion exchange chromatographic resin;
[0579] a step (xii): eluting an extract solution from the ion
exchange chromatographic resin of step (x) using a gradient solvent
solution and collecting the gradient elution fractions; and
[0580] (xiii) removing the gradient elution solution from the
produced fractions of step (xiv) to produce purified and
substantially purified extract.
[0581] In alternative embodiments, provided are methods of
substantially converting CBD to .DELTA..sup.8 THC and .DELTA..sup.9
THC comprising: [0582] (a) providing a reaction mixture comprising
a catalyst in an organic solvent; [0583] (b) adding CBD material
from steps (viii) and steps (xiii), above; [0584] (c) mixing said
reaction mixture; [0585] (d) reacting mixture for a period of time
at a controlled temperature; [0586] (e) adding a base to the
reaction mixture; [0587] (f) allowing the mixture to separate into
an aqueous phase and an organic phase; [0588] (g) removing the
organic phase; and, [0589] (h) loading the organic phase onto a
normal phase chromatography column [0590] (i) eluting the organic
phase with an organic solvent and recovering substantially pure
CBD, .DELTA..sup.8 THC and .DELTA..sup.9 THC [0591] optionally,
repeating steps (a) through (i).
[0592] In alternative embodiments, a "substantially pure"
preparation of cannabinoid is defined as a preparation having a
chromatographic purity (of the desired cannabinoid or cannabinoid
acid) of greater than about 75%, or greater than about 96%, or
greater than about 97%, or greater than about 98%, or greater than
about 99%, or greater than about 99.5%, or between about 70% and
99.9%, as determined by area normalisation of an HPLC profile.
[0593] In alternative embodiments, the term "product enriched in a
given cannabinoid" encompasses preparations having at least about
50%, or greater than about 75%, or greater than about 90%, 95% or
98%, or between about 50% and 99.9%, chromatographic purity, for
the desired cannabinoid.
[0594] In alternative embodiments, the term "about" is within 20%
of the stated value, or 19%, or 18%, or 17%, or 16%, or 15%, or
14%, or 13%, or 12%, or 11%, or 10%, or 9%, or8%, or 7%, or 6%, or
5%, or 4%, or 3%, or 2%, or 1%, or 0.5%, or 0.1%, or 0.05%, or
0.01%, or is between 20% and 0.01% of the stated value.
[0595] In alternative embodiments, a non-purified, or
non-substantially purified, product can comprise a greater
proportion of impurities, non-target materials and/or other
cannabinoids than a "substantially pure" preparation. The
cannabinoid can be (e.g., a cannabinoid purified or isolated by, or
made by a reaction of, a method as provided herein can be):
.DELTA..sup.9tetrahydrocannabinol (.DELTA..sup.8 THC);
.DELTA..sup.9tetrahydrocannabinol (.DELTA..sup.9 THC); cannabidiol
(CBD); cannabinol (CBN); cannabigerol (CBG); cannabichromene (CBC);
cannabidivarol (CBDV); tetra-hydrocannabidiol (THCBD);
tetra-hydrocannabigerol (THCBG); tetra-hydrocannabichromene
(THCBC); or, tetra-hydrocannabidivarol (THCBDV); the carboxylic
acid precursors of the foregoing compounds; and related naturally
occurring compounds and their derivatives.
[0596] In alternative embodiments, the term "cannabinoids" e g , a
cannabinoid purified or isolated by, or made by a reaction of a
process as provided herein, includes or refers to a family of
natural products that can contain a 1,1'-di-methyl-pyrane ring, a
variedly derivatized aromatic ring and/or a variedly unsaturated
cyclohexyl ring and their immediate chemical precursors.
[0597] In alternative embodiments, the term "cannflavins", e.g., a
cannflavin purified or isolated by, or made by a reaction of a
process as provided herein, includes or refers to a family of
natural products that can contain a 1,4-pyrone ring fused to a
variedly derivatized aromatic ring and linked to a second variedly
derivatized aromatic ring.
[0598] In alternative embodiments, the term "Lewis acid" refers to
a powerful electron pair acceptor; and examples include but are by
no means limited to BF.sub.3Et.sub.2O (boron trifluoride ditheyl
etherate), p-toluenesulfonic acid and boron trifluoride.
[0599] In alternative embodiments, the term "non-oxidizing acid"
refers to hydrobromic, hydrochloric, hydrofluoric, acetic, benzoic,
chloroacetic, formic, phosphoric, sulfuric, trifluroacetic and
oxalic acids.
[0600] In alternative embodiments, the term "essential oils", e.g.,
an essential oil used as a starting material in a process as
provided herein, or an essential oil that may be isolated by a
process as provided herein, includes or refers to a family of
natural products that can contain a multiple of the 5-membered
isoprene unit variedly substituted, often cyclized to form one or
more ring systems; they may also contain series of aldehydes and/or
ketones and esters of a variety of carboxylic acid substituted
compounds.
[0601] In alternative embodiments, provided are methods for
extracting and/or purifying cannabinoids from any plant material
extract known to contain such cannabinoids, cannflavins and
essential oils; and, optionally to purify cannflavins and to
optionally purify essential oils. In alternative embodiments, the
extract is passed through a series of chromatographic columns, for
example, a normal phase column, a reversed phase column or an ion
exchange column as a continuous simulated moving bed
configuration.
[0602] In one embodiment, the chromatographic column is arranged
for gradient elution fractioning using normal phase, reverse phase
and/or ion exchange chromatography. In one embodiment
.DELTA..sup.9-THC and CBD are fractionated out of the eluent. For
example, in one embodiment, as the extract is passed over the
column, .DELTA..sup.9-THC and CBD is differentially retained or
detained (e.g., reversibly bound) on the column. As a result, as
the extract comes off the column after gradient elution transition,
the initial fractions eluted off the column will be (substantially)
free of .DELTA..sup.9-THC and CBD. The fractions free of
.DELTA..sup.9-THC and CBD are pooled, thereby producing an extract
with .DELTA..sup.9-THC and CBD substantially removed (e.g., in
alternative embodiments, "substantially removed" or "substantially
fractionated" means at least 85%, 90%, 95%, 98%, 99% or 99.5% or
more removed or fractionated).
[0603] In some embodiments, (substantially) only .DELTA..sup.9-THC
is substantially fractionated out of the eluent. For example, in
one embodiment, as the extract or pooled fractions from a previous
normal phase, reverse phase and/or ion exchange elution are passed
over the column, .DELTA..sup.9-THC is differentially retained or
detained (e.g., reversibly bound) on the column. As the extracts
pooled from previous elution fractions come off the column, the
initial fractions eluted off the column will be free of
.DELTA..sup.9-THC. These fractions free of .DELTA..sup.9-THC are
pooled, thereby producing an extract with .DELTA..sup.9-THC
substantially removed (e.g., in alternative embodiments,
"substantially removed" or "substantially fractionated" means at
least 85%, 90%, 95%, 98%, 99% or 99.5% or more removed or
fractionated).
[0604] In some embodiments, when using a continuous chromatography
apparatus or device, a series of columns are arranged, for example,
3, 4, 5, 6, 7, 8, 9, or 10 or more, or between 3 and 30, columns
are arranged in a continuous rotation traveling through a series of
contact points where gradient elution solutions and extract
solution are introduced at fixed points for a period of time
allowing for continuous loading and elution, and collection of
fractions. The first column is loaded with extract solution at the
first position (or station). The first column is then moved to the
second position where the first gradient elution is introduced (or
loaded) while at the same time the second column is loaded with
extract solution at position one. The first column then rotates
(i.e., is moved) to the third position where the second gradient
solvent is introduced, the second column moves to the second
position where the first gradient solvent is introduced and the
third column is loaded with extract solution at position one. The
first column then moves to the fourth position where the third
gradient solvent is introduced, the second column moves to the
third position where the second gradient solvent is introduced, the
third column moves to the second position where the first gradient
solvent is introduced and the fourth column is loaded with extract
solution at position one. The first column then moves to the fifth
position where the fourth gradient solvent is introduced, the
second column moves to the fourth position where the third gradient
solvent is introduced, the third column moves to the third position
where the second gradient solvent is introduced, the fourth column
moves to the second position where the first gradient solvent is
introduced and the fifth column is loaded with extract solution at
position one. The first column then moves to the sixth position
where the fifth gradient solvent n is introduced, the second column
moves to the fifth position where the fourth gradient solvent is
introduced, the third column moves to the fourth position where the
third gradient solvent is introduced, the fourth column moves to
the third position where the second gradient solvent is introduced,
the fifth column moves to the second position where the first
gradient solvent is introduced and the sixth column is loaded with
extract solution at position one. The first column then moves or
returns to the first position where extract solution is loaded, the
second column moves to the sixth position where the fifth gradient
solvent is introduced, the third column moves to the fifth position
where the fourth gradient solvent is introduced, the fourth column
moves to the fourth position where the third gradient solvent is
introduced, the fifth column moves to the third position where the
second gradient solvent is introduced and the sixth column moves to
the second position where the first gradient solvent is introduced.
In a particular embodiment the first gradient solvent elutes CBD,
CBG and CBN and is substantially fractionated in the eluent. For
example, in one embodiment, as the extract or pooled fractions from
a normal phase, reverse phase and/or ion exchange elution are
passed over the column, CBD, CBG and CBN is differentially produced
in the eluent (e.g., by use of a gradient elution process). In a
particular embodiment second gradient solvent elutes CBD and is
substantially fractionated in the eluent. For example, in one
embodiment, as the extract or pooled fractions from a normal phase,
reverse phase and/or ion exchange elution are passed over the
column, CBD is differentially produced in the eluent. In a
particular embodiment the third gradient solvent elutes CBD and
.DELTA..sup.9-THC and is substantially fractionated in the eluent.
For example, in one embodiment, as the extract or pooled fractions
from a normal phase, reverse phase and/or ion exchange elution are
passed over the column, CBD and .DELTA..sup.9-THC is differentially
produced in the eluent. In a particular embodiment the fourth
gradient elutes .DELTA..sup.9-THC and is substantially fractionated
in the eluent. For example, in one embodiment, as the extract or
pooled fractions from a normal phase, reverse phase and/or ion
exchange elution are passed over the column, .DELTA..sup.9-THC is
differentially produced in the eluent. In a particular embodiment
the fifth gradient elutes CBC, THC-A, terpenes and
.DELTA..sup.9-THC and is substantially fractionated in the eluent.
For example, in one embodiment, as the extract or pooled fractions
from a normal phase, reverse phase and/or ion exchange elution are
passed over the column, CBC, THC-A, terpenes and .DELTA..sup.9-THC
is differentially produced in the eluent.
[0605] In some embodiments, when using a continuous chromatography
apparatus or device, a series of columns are arranged, for example,
3, 4, 5, 6, 7, 8, 9, or 10 or more, or between 3 and 30, columns
are arranged in a continuous rotation traveling through a series of
contact points where gradient elution solutions and extract
solution are introduced at fixed points for a period of time
allowing for continuous loading and elution, and collection of
fractions. The first column is loaded with extract solution at the
first position (or station). The first column is then moved to the
second position where the first gradient elution is introduced (or
loaded) while at the same time the second column is loaded with
extract solution at position one. The first column then rotates
(i.e., is moved) to the third position where the second gradient
solvent is introduced, the second column moves to the second
position where the first gradient solvent is introduced and the
third column is loaded with extract solution at position one. The
first column then moves to the fourth position where the third
gradient solvent is introduced, the second column moves to the
third position where the second gradient solvent is introduced, the
third column moves to the second position where the first gradient
solvent is introduced and the fourth column is loaded with extract
solution at position one. The first column then moves to the fifth
position where the fourth gradient solvent is introduced, the
second column moves to the fourth position where the third gradient
solvent is introduced, the third column moves to the third position
where the second gradient solvent is introduced, the fourth column
moves to the second position where the first gradient solvent is
introduced and the fifth column is loaded with extract solution at
position one. The first column then moves to the sixth position
where the fifth gradient solvent is introduced, the second column
moves to the fifth position where the fourth gradient solvent is
introduced, the third column moves to the fourth position where the
third gradient solvent is introduced, the fourth column moves to
the third position where the second gradient solvent is introduced,
the fifth column moves to the second position where the first
gradient solvent is introduced and the sixth column is loaded with
extract solution at position one. The first column then moves or
returns to the first position where extract solution is loaded, the
second column moves to the sixth position where the fifth gradient
solvent is introduced, the third column moves to the fifth position
where the fourth gradient solvent is introduced, the fourth column
moves to the fourth position where the third gradient solvent is
introduced, the fifth column moves to the third position where the
second gradient solvent is introduced and the sixth column moves to
the second position where the first gradient solvent is
introduced.
[0606] In a particular embodiment the first gradient solvent elutes
CBD, CBG and CBN and is substantially fractionated in the eluent.
For example, in one embodiment, as the extract or pooled fractions
from a normal phase, reverse phase and/or ion exchange elution are
passed over the column, CBD, CBG and CBN is differentially produced
in the eluent (e.g., by use of a gradient elution process). In a
particular embodiment second gradient solvent elutes CBD and is
substantially fractionated in the eluent. For example, in one
embodiment, as the extract or pooled fractions from a normal phase,
reverse phase and/or ion exchange elution are passed over the
column, CBD is differentially produced in the eluent. In a
particular embodiment the third gradient solvent elutes CBD and
.DELTA..sup.9-THC and is substantially fractionated in the eluent.
For example, in one embodiment, as the extract or pooled fractions
from a normal phase, reverse phase and/or ion exchange elution are
passed over the column, CBD and .DELTA..sup.9-THC is differentially
produced in the eluent. In a particular embodiment the fourth
gradient elutes .DELTA..sup.9-THC and is substantially fractionated
in the eluent. For example, in one embodiment, as the extract or
pooled fractions from a normal phase, reverse phase and/or ion
exchange elution are passed over the column, .DELTA..sup.9-THC is
differentially produced in the eluent. In a particular embodiment
the fifth gradient solvent elutes CBC, THC-A, terpenes and
.DELTA..sup.9-THC and is substantially fractionated in the eluent.
For example, in one embodiment, as the extract or pooled fractions
from a normal phase, reverse phase and/or ion exchange elution are
passed over the column, CBC, THC-A, terpenes and .DELTA..sup.9-THC
is differentially produced in the eluent. The second gradient
solvent containing CBD is combined with a Lewis acid or
non-oxidizing acid catalyst, optionally additional CBD or
substantially pure CBD and/or (.DELTA..sup.8-THC) and/or
.DELTA..sup.9-tetrahydrocannabinol (.DELTA.9-THC) can be added to
the reaction mixture, mixing said reaction mixture for a period of
time; adding a neutralizing agent to said mixture; filtration of
catalyst and neutralizing agent from mixture; optionally allowing
mixture to separate into an aqueous and organic phase; optionally
adding organic phase to a chromatography column and eluting the
(.DELTA.8-THC) and/or .DELTA.9-tetrahydrocannabinol (.DELTA.9-THC)
from the organic phase. The tetrahydrocannabinol may then be
combined with suitable excipients known in the art, thereby forming
a pharmaceutical composition. In one embodiment, the mixture is
allowed to separate into an aqueous phase and an organic phase; and
optionally the process further comprises removing the organic
phase.
[0607] In some embodiments, (substantially) only CBD is
substantially fractionated out of the eluent. For example, in one
embodiment, as the extract or pooled fractions from a previous
normal phase, reverse phase and/or ion exchange elution are passed
over the column, CBD is differentially retained or detained (e.g.,
reversibly bound) on the column. As a result, as the extract or
pooled previous elution fractions comes off the column, the initial
fractions eluted off the column will be (substantially) free of
CBD. These fractions free of CBD are pooled, thereby producing an
extract with CBD substantially removed (e.g., in alternative
embodiments, "substantially removed" or "substantially
fractionated" means at least 85%, 90%, 95%, 98%, 99% or 99.5% or
more removed or fractionated).
[0608] In some embodiments, .DELTA..sup.9-THC carboxylic acid
species or .DELTA..sup.9-THCA and CBD carboxylic acid species CBDA
are fractionated out of out of the eluent. For example, in one
embodiment, as the extract or pooled fractions from a first elution
are passed over the column, .DELTA..sup.9-THCA and CBDA are
differentially retained or detained (e.g., reversibly bound) on the
column. As a result, as the extract or pooled first elution
fractions comes off the column, the initial fractions eluted off
the column will be (substantially) free of .DELTA..sup.9-THCA and
CBDA. These fractions (substantially) free of .DELTA..sup.9-THCA
and CBDA are pooled, thereby producing an extract with
.DELTA..sup.9-THCA and CBDA substantially removed (e.g., in
alternative embodiments, "substantially removed" or "substantially
fractionated" means at least 85%, 90%, 95%, 98%, 99% or 99.5% or
more removed or fractionated).
[0609] In some embodiments, the .DELTA..sup.9-THC may be eluted
from the column, extracted or concentrated, for purifying, or
substantially purifying, .DELTA..sup.9-THC. In alternative
embodiments, the chromatographic column is arranged for
fractionating (e.g., sequentially fractionating) a specific
cannabinoid or groups of cannabinoids or their carboxylic acid
species, cannflavin or essential oil or class of cannabinoids,
cannflavins or essential oils out of the eluent, for example,
cannabidiol (CBD), cannabinol (CBN), cannabigerol (CBG),
cannabichromene (CBC), cannabidivarol (CBDV), tetrahydrocannabidiol
(THCBD), tetrahydrocannabigerol (THCBG), tetrahydrocannabichromene
(THCBC), tetrahydrocannabidivarol (THCBDV), A.sup.8-THC, the
carboxylic acid precursors of the foregoing compounds, and related
naturally occurring compounds and their derivatives. In alternate
embodiments, the chromatographic column is arranged for
fractionating (e.g., sequentially fractionating) cannflavins and
related naturally occurring compounds and their derivatives. In
alternate embodiments, the system is arranged to fractionate the
components of essential oils. The list of compounds provided herein
is not exhaustive and is in no way intended to be limiting. In
these embodiments, the compound(s) of interest are retained or
detained (e.g., reversibly bound) on the column so that fractions
(alternatively, the last fractions) of the extract eluted from the
column contain the compounds(s) of interest. In alternative
embodiments, fractions containing the compound(s) of interest are
pooled. In some embodiments, different compounds may be extracted
with different solvents and then combined into a single extract. As
will be appreciated by one knowledgeable in the art, in this
manner, several different cannabinoids could be purified from a
single extract.
[0610] In alternative embodiments, the "plant material" or
botanical drug substance is derived from one or more cannabis or
hemp plants, or from other plants, or a microbial source, including
yeast, bacteria, lichen, algae. The term "plant material"
encompasses a plant or plant part (e.g. bark, wood, leaves, stems,
roots, flowers, fruits, seeds, berries or parts thereof) as well as
exudates, and includes material falling within the definition of
"botanical raw material" in the Guidance for Industry Botanical
Drug Products Draft Guidance, August 2000, US Department of Health
and Human Services, Food and Drug Administration Centre for Drug
Evaluation and Research. The term "cannabis plant(s)" encompasses
wild type Cannabis sativa and also variants thereof, including
Cannabis chemovars (varieties characterized by virtue of chemical
composition) which naturally contain different amounts of the
individual cannabinoids, also Cannabis sativa subspecies indica
including the variants var. indica and var. kafiristanica, Cannabis
indica and also plants which are the result of genetic crosses,
self-crosses or hybrids thereof. The term "Cannabis plant material"
also can encompass plant material derived from one or more cannabis
plants, and can comprise any "cannabis plant material" including,
e.g., herbal Cannabis and dried Cannabis biomass. The term
"Cannabis plant material" also can encompass "decarboxylated
Cannabis plant material", which refers to Cannabis plant material
which has been subject to a decarboxylation step in order to
convert cannabinoid acids to the corresponding free
cannabinoids.
[0611] In alternative embodiments, a starting material for a
purification process as provided herein is an extract solution
containing a cannabinoid or cannabinoid acid obtained from a
natural or a synthetic source, e.g., a plant or a microbial
material. In alternative embodiments, the "extract solution
containing a cannabinoid or cannabinoid acid" comprises a solvent
extract of a plant material. Solvents used for extraction for use
in the preparation of extract solutions can comprise non-polar
solvents, polar solvents such as ethanol, methanol or water, or
organic solvents such as liquid carbon dioxide, and combinations
thereof. The solvent may be an organic solvent, selected from the
group consisting of: non-polar solvents include liquid non-polar
solvents comprising lower C1-C12, for example, C3 to C8, or
straight chain or branched chain alkanes, for example, methane,
ethane, propane, butane, pentane, toluene, trimethylpentane; a low
molecular weight alcohol, polar solvents comprising, for example,
ethanol, methanol, water; a low molecular weight chlorinated
hydrocarbon, for example, chloroform and dichloromethane or
mixtures thereof; or a supercritical fluid such as CO.sub.2 with or
without an organic solvent modifier.
[0612] In alternative embodiments, the extract is prepared by
dissolving or partially dissolving natural or synthetic, or the
plant or microbial material, in a solvent, removing insoluble
material from the resultant solution (optionally by filtration with
or without activated carbon, precipitation, centrifugation and the
like), and optionally removing some or all of the extraction
solvent from the solution (optionally by evaporation, e.g., rotary
evaporation) to form an extract or extract solution or concentrate
containing a cannabinoid or cannabinoid acid.
[0613] In alternative embodiments, extractions can comprise using a
technique referred to as accelerated solvent extraction or may use
subcritical water or any combination of water and solvent. In one
embodiment, when isolating cannabinoid acids, a modified pH
gradient elution solution is used. The primary purpose of this pH
adjustment (the modified pH gradient) is to promote or prevent
ionization of the cannabinoid acid. pH modified gradient elution
solutions may be achieved by the additional of a small volume of
acid or base to the solvent. It may be sufficient to add a
relatively weak acid, such as acetic acid, oxalic acid, glycolic
acid, carbonic acid or ammonium hydroxide or a small amount of base
or buffering agent such as sodium hydroxide, magnesium hydroxide,
sodium carbonate or sodium bicarbonate. For any given purification
process the optimal amount and type of acid or base used may be
determined empirically. An alternative exemplary acidified solvent
is 0.1% acetic acid in ethanol or 0.1% sodium hydroxide in ethanol.
In alternative embodiments, the neutralizing agent consists of for
example sodium hydroxide, sodium carbonate, potassium carbonate,
and potassium t-amylate, sodium bicarbonate.
[0614] Acidified non-polar and polar solvents of the types
described above can be useful in preparation of gradient elutions
using ion exchange chromatography. The solvents used in the
conversion can comprise an organic solvent, e.g., a non-polar
solvent, including a liquid non-polar solvent comprising lower
C1-C12, or C3 to C8, straight chain or branched chain alkanes for
example, methane, ethane, propane, butane, pentane, toluene,
trimethylpentane, hexane; a low molecular weight alcohol, polar
solvents consisting of for example, ethanol; a low molecular weight
chlorinated hydrocarbon, for example, chloroform and
dichloromethane; a heterocyclic compound or cyclic ether for
example, tetrahydrofuran and 2-Methyltetrahydrofuran and aromatic
ring hydrocarbons such as benzene, toluene, xylene and
ethylbenzene.
[0615] In alternative embodiments, the plant material is subjected
to a decarboxylation step prior to solvent extraction. The purpose
of the decarboxylation step is to convert cannabinoid acids present
in the plant material to the corresponding free cannabinoids. In
alternative embodiments, the decarboxylation is carried out by
heating the plant material to a defined temperature for a suitable
length of time. Decarboxylation of cannabinoid acids is a function
of time and temperature, thus at higher temperatures a shorter
period of time will be taken for complete decarboxylation of a
given amount of cannabinoid acid. In alternative embodiments
selecting appropriate conditions for decarboxylation consideration
include minimizing thermal degradation of the desirable,
pharmacological cannabinoids into undesirable degradation products,
particularly thermal degradation of .DELTA..sup.9-THC. In
alternative embodiments, the decarboxylation is carried out in a
multi-step heating process in which the plant material is: i)
heated to a first temperature for a first (relatively short) time
period to evaporate off retained water and allow for uniform
heating of the plant material; and ii) the temperature is increased
to a second temperature for a second time period (typically longer
than the first time period) until at least 95% conversion of the
acid cannabinoids to their neutral form has occurred.
[0616] In alternative embodiments, the "extract containing a
cannabinoid or a cannabinoid acid" prepared from the starting plant
material comprises a "botanical drug substance" prepared from the
plant material, or a polar or non-polar solvent solution of such a
botanical drug substance. In the context of this application a
"botanical drug substance" is an extract derived from plant
material, which extract fulfills the definition of "botanical drug
substance" provided in the Guidance for Industry Botanical Drug
Products Draft Guidance, August 2000, US Department of Health and
Human Services, Food and Drug Administration Centre for Drug
Evaluation and Research of: "A drug substance derived from one or
more plants, algae, or macroscopic fungi. It is prepared from
botanical raw materials by one or more of the following processes:
pulverization, decoction, expression, aqueous extraction, ethanolic
extraction, or other similar processes." "Botanical drug
substances" derived from cannabis plants include primary extracts
prepared by such processes as, for example, maceration,
percolation, and solvent extraction.
[0617] In alternative embodiments, solvent extraction may be
carried out using essentially any solvent that dissolves, or
substantially dissolves, cannabinoids/cannabinoid acids, such as
for example C1 to C.sub.5 alcohols (e.g. ethanol, methanol), C5-C12
alkanes (e.g. hexane), norflurane (HFA134a),
1,1,1,2,3,3,3-Heptafluoropropane (or HFA227), chloroform,
dichloromethane, dichloroethane and carbon dioxide. When solvents
such as those listed above are used, the resultant extract
typically contains non-specific lipid-soluble material. This can
optionally be removed by a variety of processes including
filtration to remove solids, "winterization", which involves for
example chilling to -20.degree. C. or lower followed by filtration
to remove waxy ballast, extraction with liquid carbon dioxide and
by distillation.
[0618] In alternative embodiments, any protocol for the preparation
of botanical drug substances from cannabis and hemp plant material
can be used, e.g., as described in International patent application
WO 02/064109. In alternative embodiments, the botanical drug
substance is obtained by carbon dioxide (CO.sub.2) extraction,
polar solvent extraction or non-polar solvent extraction or
combinations thereof followed by a filtration. Optionally a
secondary extraction is performed to remove a substantial
proportion of non-cannabinoid materials, e.g. waxes, wax esters and
glycerides, unsaturated fatty acid residues, terpenes, carotenes,
and flavonoids and other ballast.
[0619] In alternative embodiments, if it is intended to prepare
free cannabinoids from the plant or microbial material, e.g.,
cannabis, then the material is heated to a defined temperature for
a defined period of time in order to partially or substantially
decarboxylate cannabinoid acids to free cannabinoids prior to
extraction of the botanical drug substance. In alternative
embodiments, the botanical drug substance is prepared according to
a process comprising the following steps: i) optional
decarboxylation of the plant material, ii) extraction with polar or
non-polar solvent, to produce a crude botanical drug substance,
iii) optional precipitation with C1-C5 alcohol to reduce the
proportion of non-target materials, iv) removal of the precipitate
(for example, by filtration, precipitation, centrifugation and the
like), v) optional treatment with activated charcoal, and vi)
evaporation to remove C1-C5 alcohol and water, thereby producing a
final botanical drug substance.
[0620] In alternative embodiments, provided are methods for
converting substantially purified cannabidiol (CBD) to
.DELTA..sup.8-tetrahydrocannabinol (.DELTA..sup.8THC) and
.DELTA..sup.9-tetrahydrocannabinol (.DELTA.9-THC). As will be
appreciated by one knowledgeable in the art and as discussed below,
the reaction times may be varied somewhat, producing product at
different yields and purities. Furthermore, functional equivalents
may be substituted where appropriate.
[0621] In alternative embodiments, an exemplary method of
converting CBD to .DELTA..sup.8-tetrahydrocannabinol
(.DELTA..sup.8-THC) and .DELTA..sup.9-tetrahydrocannabinol
(.DELTA..sup.9-THC) comprises: providing a reaction mixture
comprising a Lewis acid or non-oxidizing acid catalyst in a
reaction solvent, adding a substantially pure CBD or substantially
pure CBD and/or (.DELTA..sup.8-THC) and/or
.DELTA..sup.9-tetrahydrocannabinol (.DELTA.9-THC) to the reaction
mixture, mixing said reaction mixture for a period of time; adding
a neutralizing agent to said mixture; filtration of catalyst and
neutralizing agent from mixture; optionally allowing mixture to
separate into an aqueous and organic phase; optionally removing the
reaction solvent; optionally dissolving organic phase in a second
solvent; adding organic phase to a chromatography column and
eluting the tetrahydrocannabinol from the organic phase. The
tetrahydrocannabinol may then be combined with suitable excipients
known in the art, thereby forming a pharmaceutical composition. In
one embodiment, the mixture is allowed to separate into an aqueous
phase and an organic phase; and optionally the process further
comprises removing the organic phase).
[0622] In alternative embodiments, the tetrahydrocannabinol at
therapeutically effective concentrations or dosages is combined
with a pharmaceutically or pharmacologically acceptable carrier,
excipient or diluent, either biodegradable or non-biodegradable.
Exemplary examples of carriers include, but are by no means limited
to, for example, poly(ethylene-vinyl acetate), copolymers of lactic
acid and glycolic acid, poly(lactic acid), gelatin, collagen
matrices, polysaccharides, poly(D,L lactide), poly(malic acid),
poly(caprolactone), celluloses, albumin, starch, casein, dextran,
polyesters, ethanol, methacrylate, polyurethane, polyethylene,
vinyl polymers, glycols, mixtures thereof and the like. Standard
excipients that can be used include gelatin, casein, lecithin, gum
acacia, cholesterol, tragacanth, stearic acid, benzalkonium
chloride, calcium stearate, glyceryl monostearate, cetostearyl
alcohol, cetomacrogol emulsifying wax, sorbitan esters,
polyoxyethylene alkyl ethers, polyoxyethylene castor oil
derivatives, polyoxyethylene sorbitan fatty acid esters,
polyethylene glycols, polyoxyethylene stearates, colloidol silicon
dioxide, phosphates, sodium dodecyl sulfate, carboxymethylcellulose
calcium, carboxymethylcellulose sodium, methylcellulose,
hydroxyethylcellulose, hydroxypropylcellulose,
hydroxypropylmethycellulose phthalate, non-crystalline cellulose,
magnesium aluminum silicate, triethanolamine, polyvinyl alcohol,
polyvinylpyrrolidone, sugars and starches. See, for example,
Remington: The Science and Practice of Pharmacy, 1995, Gennaro
ed.
[0623] In some embodiments, the catalyst is a Lewis acid, for
example, p-toluenesulfonic acid, boron trifluoride or
BF.sub.3Et.sub.2O. In some embodiments, the BF.sub.3Et.sub.2O
(boron trifluoride diethyl etherate) is in dry methylene chloride,
ethyl acetate, ethanol, hexane or other organic solvent. In yet
other examples, the catalyst may be hydrochloric acid in ethanol or
sulfuric acid in cyclohexane.
[0624] In some embodiments, the catalyst is a non-oxidizing acid,
for example, formic acid, acetic acid or hydrobromic acid. In some
embodiments, the non-oxidizing acid is in dry methylene chloride,
ethyl acetate, ethanol, hexane or other organic solvent.
[0625] In some embodiments, a base is added to the reaction mixture
prior to optionally allowing the reaction mixture to separate into
organic and aqueous phases. The base may be an alkali metal
hydrogen carbonate, carbonate of an alkali metal, lithium hydroxide
(LiOH), sodium hydroxide (NaOH), potassium hydroxide (KOH),
rubidium hydroxide (RbOH), cesium hydroxide (CsOH), magnesium
hydroxide (Mg(OH)), calcium hydroxide (Ca(OH)), strontium hydroxide
(Sr(OH)), barium hydroxide (Ba(OH)).
[0626] In some embodiments, the organic layer is dried prior to
eluting. In these embodiments, a suitable drying or dehydration
compound, for example, MgSO4 or Na.sub.2SO.sub.4 is used.
[0627] In yet other embodiments, the process may be carried out
under an inert atmosphere such as a nitrogen (e.g., N.sub.2)
atmosphere.
[0628] In alternative embodiments, and as discussed below, yield is
determined by looking at the peak area for the isolated compound in
the gas chromatography mass spectra analysis of the crude reaction
product mixture and the final reaction product mixture. It is
important to note that in the prior art, yield is often calculated
on the basis of first isolated crude product before final
purification. In some embodiments of processes provided herein
yield of .DELTA.8-THC and .DELTA.9-THC is at least about 75%; in
other embodiments, the yield of .DELTA.8-THC and .DELTA.9-THC is at
least about 90%; and in other embodiments, yield of .DELTA.8-THC
and .DELTA.9-THC is at least about 98%; and in yet other
embodiments, yield of .DELTA.8-THC and .DELTA.9-THC is between
about 75 to 98% or 99%.
[0629] In alternative embodiments, purity is determined by Gas
Chromatography Mass Spectrometry (GC-MS) and/or by analytical
high-performance liquid chromatography (HPLC). The total ion
chromatogram from the GC-MS gives information similar to that
provided by a flame ionization detector (FID)-GC in that the peak
area is proportional to the mass of the analytes detected. Total
peak area and the peak areas of the individual analytes can be
compared in the GC-MS case as long as the masses are in generally
the same range. As discussed below, in some embodiments, purity of
the .DELTA.8-THC and .DELTA.9-THC mixture isolated by the process
is greater than about 90%, 95%, 97% or 98%, or purity is greater
that about 98% to 99%.
Continuous Chromatography Apparatus or Devices
[0630] In alternative embodiments, processes and methods as
provided herein comprise use of continuous chromatography apparatus
or devices, and associated robots and software. Any continuous
chromatography apparatus or device known in the art can be used,
e.g., as described in: Challener, BioPharm International, Vol 31,
Issue 4, pg 14-18; Zobel et al Ind. Eng. Chem. Res., 2014, 53 (22),
pp 9169-9185; Horvath et al Org. Process Res. Dev., 2015,19 (6), pp
624-634; or as manufactured e.g., by Ionex, Calgon, ThermoFischer
Scientific.
[0631] Embodiments as provided herein will be further described
with reference to the Examples described herein; however, it is to
be understood that the invention is not limited to such
examples.
EXAMPLES
Example 1: Exemplary Methods
[0632] In alternative embodiments, the "extract, aliquot or sample
comprising or containing a cannabinoid or a cannabinoid acid" is
dissolved in a gradient elution solution and subjected to a
chromatographic purification step to produce substantially pure
cannabinoids and a product enriched in a given cannabinoid. The
purpose of this step is to first remove the targeted
non-cannabinoid material comprising for example terpenes,
carotenes, and flavonoids and also to provide a degree of
separation/fractionation of the various cannabinoid/cannabinoid
acid components of the extract.
[0633] In alternative embodiments, the product of the gradient
elution chromatographic step is collected in multiple fractions,
which may then be tested for the presence of the desired
cannabinoid/cannabinoid acid using any suitable analytical
technique. Fractions enriched in the desired cannabinoid and/or
cannabinoid acid may then be selected for further purification.
Optionally the gradient elution solution is removed from the
selected fractions or optionally additional gradient solvent
solution is added to change the polarity or hydrogen potential of
the gradient solvent solution contained in the selected fraction.
The selected fraction can then be subjected to one or more
additional chromatographic steps and one or more gradient elution
solutions modifications.
[0634] In alternative embodiments, the chromatographic step
comprises a column chromatography in a fixed or a continuous mode,
and is can be based on molecular sizing, polarity and/or hydrogen
potentiality. In alternative embodiments, the column matrix
materials are, for example; silica (or silica gel) and/or alumina
normal phase and reverse phase such as for example C18, C8, C4, C2,
amino, cyano, phenyl, diol, WAX, SAX, WCX, SCX, Thiol; acidic,
basic and neutral, styrenic, brominated styrenic; ion exchange
resins such as strongly acidic, typically featuring sulfonic acid
groups, e.g. sodium polystyrene sulfonate or polyAMPS; strongly
basic, typically featuring quaternary amino groups, for example,
trimethylammonium groups, e.g. polyAPTAC); weakly acidic, typically
featuring carboxylic acid groups; weakly basic, typically featuring
primary, secondary, and/or tertiary amino groups, e.g. polyethylene
amine.
[0635] In alternative embodiments, various different elution
solutions are used in combination with this type of matrix, for
example dimethyl sulfoxide, pyridine water, dimethylformamide,
methanol, ethylene dichloride, chloroform, propanol, ethanol,
isobutanol, formamide, methylene dichloride, butanol, isopropanol,
tetrahydrofuran, dioxane, hexane, butane, pentane, heptane, octane,
carbon tetrachloride, etc.
[0636] In alternative embodiments, the chromatographic step
comprises column chromatography on for example, a silica (or silica
gel) or an alumina column, and the step can comprise eluting with a
5:1; 4:1; 3:1; 2:1; 1.9:1; 1.8:1; 1.7:1; 1.6:1; 1.5:1; 1.4:1;
1.3:1; 1.2:1; 1.1:1; 1.01:1; 1:1; 0.9:1; 0.5:1; 0.1:1 mixture of
elution solutions such as chloroform, methylene dichloride,
ethylene dichloride, methanol, ethanol, propanol and/or water. Any
suitable combination of normal phase column packing material and
solvent having separation characteristics suitable for use in
separation (fractionation) of cannabinoids and/or cannabinoid acids
can be used with equivalent effect.
[0637] In alternative embodiments, the column gradient eluate is
collected in several fractions. The fractions are tested for the
presence of the desired cannabinoid and/or cannabinoid acid using a
suitable analytical technique, and those fractions containing the
highest amounts of the desired cannabinoid and/or cannabinoid acid
selected for further processing. Solvent can be then removed from
the selected fractions, for example, by evaporation, e.g., rotary
evaporation or equivalents.
[0638] In alternative embodiments, the fractions enriched in a
given cannabinoid obtained from the gradient elution normal phase
chromatographic step are mixed with a solvent of the opposite
polarity thereby causing a concentration of the cannabinoids in the
second solvent phase. In alternative embodiments, the solvent is
then removed.
[0639] In alternative embodiments, the fractions containing a
product enriched in a given cannabinoid obtained from the gradient
elution normal phase chromatographic step or steps are re-dissolved
in a gradient elution solution. In alternative embodiments, the
fractions containing a product enriched in a given cannabinoid such
as mixtures comprising CBD and THC, or mixtures of CBD and CBG or
mixtures of CBG and CBC, are dissolved in a gradient elution
solution and subjected to a reverse phase chromatographic
purification step to produce substantially pure extracts.
[0640] In alternative embodiments, the product of the reverse phase
chromatographic step is collected in multiple fractions, which may
then be tested for the presence of the desired cannabinoid and/or
cannabinoid acid using any suitable analytical technique. Fractions
enriched in the desired cannabinoid or cannabinoid acid may then be
selected for further purification. In alternative embodiments, the
elution solution is removed from the selected fractions or
optionally additional solution is added to change the polarity of
the solution contained in the selected fraction.
[0641] An exemplary embodiment comprises: fractions enriched in a
given cannabinoid obtained from the elution of a reverse phase
chromatographic step are mixed with a solvent of the opposite
polarity thereby causing a concentration of the cannabinoids in the
second solvent phase. In alternative embodiments, the solvent is
then removed. In alternative embodiments, the selected fraction is
then subjected to one or more additional reverse phase
chromatographic steps and one or more elution solutions
modifications. In alternative embodiments, the reverse phase
chromatographic step comprises a column chromatography in fixed or
continuous mode, and can be based on polarity. Examples of reverse
phase column chromatography include but are not limited to pure
silica, alkyl chain-bonded silica, cyano-bonded silica, and
phenyl-bonded silica.
[0642] In alternative embodiments, various different elution
solutions may be used in combination with this type of matrix, for
example dimethyl sulfoxide, water, dimethylformamide, methanol,
saline chloroform, propanol, ethanol, isobutanol, formamide,
butanol, isopropanol, tetrahydrofuran, dioxane, dichloromethane,
dichloroethane, etc.
[0643] In alternative embodiments, the chromatographic step
comprises column chromatography using an ion exchange resin such as
but not limited to RediSep.RTM. Rf SAX, Dowex.RTM. Marathon C Na,
Marathon C H, Marathon MSC-1 or Dowex Marathon WBATM column, and
eluting can be with a 20:1; 15:1; 10:1, 5:1; 4.5:1; 4:1; 3.5:1; 3:1
2:1; 1.9:1; 1.8:1; 1.7:1; 1.6:1; 1.5:1; 1.4:1; 1.3:1; 1.2:1; 1.1:1;
1.01:1; 1.001:1; 0.1:1 mixture of elution solvents such as
chloroform, dichloromethane, dichloroethane, ethanol, propanol,
dimethyl sulfoxide, water, dimethylformamide, methanol, saline
chloroform, propanol, ethanol, isobutanol, formamide, butanol,
isopropanol, tetrahydrofuran, dioxane, dichloromethane,
dichloroethane, acetic acid, carbonic acid, glycolic acid, benzoic
acid, formic acid, oxalic acid, sodium hydroxide, potassium
hydroxide, ammonium hydroxide, ammonium carbonate, and the like,
and/or water. Any suitable combination of ion exchange phase column
packing material and solvent having separation characteristics
suitable for use in purification of cannabinoids and cannabinoids
acid can be used with equivalent effect.
[0644] In alternative embodiments, the column eluate is collected
in several fractions. In alternative embodiments, the fractions are
tested for the presence of the desired cannabinoid or cannabinoid
acid using a suitable analytical technique, and those fractions
containing the highest amounts of the desired cannabinoid or
cannabinoid acid. In alternative embodiments, solvent is then
removed from the selected fractions, optionally by evaporation,
e.g., rotary evaporation.
[0645] In alternative embodiments, the fractions containing a
product are enriched in a given cannabinoid acid obtained from the
elution chromatographic step or steps, and the fractions containing
a product enriched in a given cannabinoid acid obtained from
elution reverse phase step or steps are re-dissolved in an elution
solution. In alternative embodiments, the fractions containing a
product enriched in a given cannabinoid acid such as
tetrahydrocannabinolic acid (THCA), are dissolved in an elution
solution and subjected to an ion exchange chromatographic
purification step to produce substantially pure extracts. In
alternative embodiments, the product of the ion exchange
chromatographic step is collected in multiple fractions, which may
then be tested for the presence of the desired cannabinoid or
cannabinoid acid using any suitable analytical technique. Fractions
enriched in the desired cannabinoid or cannabinoid acid may then be
selected for further purification. In alternative embodiments, the
elution solution is removed from the selected fractions or
optionally additional solution is added to change the polarity
and/or hydrogen potential of the solution contained in the selected
fraction. An exemplary embodiment comprises having the fractions
enriched in a given cannabinoid obtained from the elution ion
exchange chromatographic step mixed with a solvent of the opposite
polarity thereby causing a concentration of the cannabinoids in the
second solvent phase. The solvent can then be removed.
[0646] In alternative embodiments, the selected fraction is then
subjected to one or more additional ion exchange chromatographic
steps and one or more isocratic or gradient elution solutions
modifications. The ion exchange chromatographic step can comprise
column chromatography in fixed or continuous mode, and can be based
on molecular sizing and polarity ion exchange resin such as but not
limited to RediSep.RTM. Rf SAX, Dowex.RTM. Marathon C Na, Marathon
C H, Marathon MSC-1 or Dowex Marathon WBA.TM.
[0647] In alternative embodiments, various different elution
solutions may be used in combination with this type of matrix, for
example dimethyl sulfoxide, water, dimethylformamide, methanol,
ethylene dichloride, chloroform, propanol, ethanol, isobutanol,
formamide, methylene dichloride, butanol, isopropanol,
tetrahydrofuran, dioxane, acetic acid, carbonic acid, glycolic
acid, benzoic acid, formic acid, oxalic acid, sodium hydroxide,
potassium hydroxide, ammonium hydroxide, ammonium carbonate,
etc.
[0648] In alternative embodiments, essential features of the
process are the same for purification of all cannabinoids and
cannabinoid acids. Cannabis plants generally contain complex
mixtures of cannabinoid acids and cannabinoids, although depending
on the variety of cannabis one type of cannabinoid may
pre-dominate. The purpose of the elution (e.g., gradient elution)
chromatographic steps (ii) is to separate the various cannabinoid
and/or cannabinoid/cannabinoid acid components of the crude plant
extract loaded in step (i), as described above, into substantially
pure fractions or substantially pure mixtures of fractions which
are then optionally subjected to isomerization reactions and/or
elimination reactions and/or additional gradient elution
chromatography comprising normal phase, reverse phase and/or ion
exchange.
[0649] In alternative embodiments, the product of the
chromatographic step is collected in multiple fractions, which may
then be tested for the presence of the desired
cannabinoid/cannabinoid acid using any suitable analytical
technique. Fractions enriched in the desired
cannabinoid/cannabinoid acid may then be selected for further
purification. Hence, the same simple process steps may be adapted
for purification of essentially any plant-derived cannabinoid or
cannabinoid acid.
[0650] Selectivity for different cannabinoids or cannabinoid acids
may be enhanced by selection of appropriate starting plant
material. By way of example, if it is desired to prepare
substantially pure .DELTA..sup.9 THC or .DELTA..sup.9 THCA then
"high THC" cannabis plants can be selected as the starting
material. Alternatively, if it is desired to prepare substantially
pure CBD or CBDA then "high CBD" cannabis plants can be selected as
the starting material. It is to be understood that the processes as
provided herein are of general utility and are not limited to the
use of particular Cannabis varieties as the starting material. The
precise cannabinoid content of any particular Cannabis plant
material can be qualitatively and quantitatively determined using
analytical techniques well known to those skilled in the art, such
as thin-layer chromatography or high-performance liquid
chromatography (HPLC). Thus, one can screen a range of various
Cannabis plants and select those having a high content of the
desired cannabinoid acid or cannabinoid for use as starting
material in a process as provided herein.
[0651] With the use of conventional selective breeding techniques
it is possible to develop cannabis varieties (chemovars) having
varying cannabinoid content. Select cannabis varieties (chemovars)
have relatively high content of CBD, or of the minor cannabinoids
.DELTA..sup.9-tetrahydrocannabivarin (.DELTA..sup.9-THCV),
cannabigerol (CBG) or cannabichromene (CBC). General protocols for
growing of medicinal cannabis and for testing the cannabinoid
content of cannabis plants are described in International patent
application WO 02/064109.
[0652] In alternative embodiments, methods further provide for the
generation of a substantially pure preparation of .DELTA..sup.9 THC
having a chromatographic purity of greater than about 75%, 80%,
90%, 95%, 96%, 97%, 98%, or 99% or more by area normalization of an
HPLC profile. The preparation can be a semi-solid at room
temperature. The preparation can comprise less than about 1.5%,
less than about 0.4%, or less than about 0.2%; or, less than about
0.1% CBD (w/w), less than about 0.5%, less than about 0.4%, or less
than about 0.2%, or less than about 0.1% CBD (w/w) as analyzed by
HPLC.
[0653] In alternative embodiments, the pure .DELTA..sup.8-THC and
.DELTA..sup.9-THC provided by exemplary methods as provided herein
have utility as an active pharmaceutical agent, and is also useful
as a chromatographic standard, particularly as a comparative
standard in the qualitative analysis of botanical drug substances
derived from cannabis. The availability of highly pure
.DELTA..sup.8-THC and .DELTA..sup.9-THC will also facilitate
studies of the pharmacology of .DELTA..sup.8-THC and
.DELTA..sup.9-THC mixtures.
[0654] In alternative embodiments, an exemplary method for
preparation of substantially pure .DELTA..sup.8-THC and
.DELTA..sup.9 THC comprises: [0655] i) obtaining an ethanolic
solution of a botanical drug substance from cannabis or hemp plant
material, [0656] ii) passing the solution obtained in step i)
through a filter, and collecting the eluate, [0657] iii) optionally
substantially remove solvent from the eluate by evaporation, e.g.,
rotary evaporation, to give a cannabinoid enriched fraction, [0658]
iv) optionally adding solvent to the eluate, [0659] v) passing a
solution of the resulting cannabinoid enriched extract through a
column packed with a stationary phase resin such as DOWEX.RTM.
MARATHON C Na.TM., MARATHON C H.TM., and conducting a gradient
elution of50:1;20:1;10:1; 9:1; 8:1; 7:1; 6:1; 5:1; 4:1; 3:1; 2:1;
1;1;0.5:1;0.005:1;0.0005:1 chloroform/methanol or ethanol, [0660]
vi) adjusting hydrogen potential of said solution, [0661] vii)
collecting .DELTA..sup.9-THC and CBD enriched fractions and
optionally removing solvent, e.g., by evaporation, e.g., rotary
evaporation, [0662] viii) mixing the collected .DELTA..sup.9 THC
and CBD with a reaction solvent and a catalyst for a period of time
and adding a neutralizing agent and removing catalyst and
neutralizing agent by filtration to generate a reduced CBD and
enriched .DELTA..sup.8-THC and .DELTA..sup.9-THC mixture,
optionally removing reaction solvent, [0663] ix) mixing reduced CBD
and enriched .DELTA..sup.8-THC and .DELTA..sup.9-THC mixture with
optionally additional reaction solvent and a stabilizing agent,
mixing stabilizing agent for a period of time [0664] x) optionally
removing the reaction solvent, [0665] x) optionally re-dissolving
the enriched .DELTA..sup.8 THC, .DELTA..sup.9 THC and reduced CBD
prepared in steps viii) through x) and passing the solution through
a column packed with reverse phase resin, and conducting a gradient
elution of 10:1; 9:1; 8:1; 7:1; 6:1; 5:1; 4:1; 3:1; 2:1; 1;1
methanol/water, [0666] xiii) optionally re-dissolving the crude
.DELTA..sup.8 THC, .DELTA..sup.9 THC and reduced CBD prepared in
steps viii) through x) and passing the solution through a column
packed with an ion exchange resin such as DOWEX.RTM. MARATHON C
Na.TM., MARATHON C H.TM., and conducting an elution of 50:1; 20:1;
10:1; 9:1; 8:1; 7:1; 6:1; 5:1; 4:1; 3:1; 2:1; 1;1; 0.5:1; 0.005: 1;
0.0005:1 chloroform and polar solvent mixture. [0667] xiv)
adjusting hydrogen potential of said solution [0668] xv) collecting
the THC enriched fractions and removing solvent, e.g., by
evaporation, e.g., rotary evaporation, to give a semi-solid
preparation of THC.
Example 2: Conversion of CBD to .DELTA..sup.8-THC and
.DELTA..sup.9-THC
[0669] This Example describes an exemplary method for the
conversion of CBD to .DELTA..sup.8-THC and .DELTA..sup.9-THC.
[0670] CBD (1 g) was added to 6.4 ml of reaction solvent and 1%
p-toluenesulfonic acid. In this example, the mixture was reacted
for 48 hours, although other time periods can also be used, as
discussed below. It was then diluted with ether (20 ml) and poured
into water, the upper layer was separated, washed with aqueous 5%
NaHCO.sub.3, then with water, dried over MgSO.sub.4 and evaporated.
GC-MS analysis on the crude product, showed the presence of 1.1%
CBD; 18.4% .DELTA..sup.8-THC and 80.0% .DELTA..sup.9-THC. The crude
product was then subjected to column chromatography. In the example
described above, normal phase HPLC separation is used wherein the
column is for example a silica gel and the mobile phase is an
organic solvent mixture introduced as a gradient described in the
invention. In other embodiments, reverse phase HPLC separation is
used.
[0671] The p-toluenesulfonic acid is used as a catalyst in the
above example. It is of note that boron trifluoride could also be
used as a catalyst, as could a number of other Lewis acids or
non-oxidizing acid catalysts. The exact proportion is not essential
to the reaction proceeding. Other solvents can also be used, for
example, benzene, toluene, chloroform, dichloromethane, etc.
[0672] In other embodiments, anhydrous MgSO.sub.4 or another
suitable agents such as Na.sub.2SO.sub.4, CaSO.sub.4, and
CaCl.sub.2, known in the art is used in place of the
MgSO.sub.4.
Example 3: Conversion of CBD to .DELTA..sup.8-THC and
.DELTA..sup.9-THC
[0673] This Example describes an exemplary method for the
conversion of CBD to .DELTA..sup.8-THC and .DELTA..sup.9-THC.
[0674] A hemp extract was obtained by extraction of hemp in
ethanol. The hemp extract was composed of CBD, THC, cannabinoids
and other components and subjected to conversion of CBD to THC
without further purification. The hemp extract and 1.about.5 mol %
p-toluenesulfonic acid were sequentially added to 6.4 mL reaction
solvent. In this example, the reaction mixture was stirred at room
temperature for 24 hours, although other time periods can also be
used, as discussed below. At some time intervals, the reaction
mixture was diluted with ether (20 mL) and poured into water. The
upper layer was separated, washed with aqueous 5% NaHCO.sub.3,
dried over MgSO.sub.4 and concentrated on a rotary evaporator.
GC-MS analysis on the crude product showed the conversion of CBD to
.DELTA..sup.8-THC and .DELTA..sup.8-THC, for example, 0% CBD; 98%
.DELTA..sup.9-THC; 2% .DELTA..sup.8-THC after 24 hours in the
presence of 3 mol % p-toluenesulfonic acid. The crude product was
then subjected to column chromatography. In the example described
above, normal phase HPLC separation is used wherein the column is
for example a silica gel and the mobile phase is an organic solvent
mixture introduced as a gradient described in the invention. In
other embodiments, reverse phase HPLC separation is used.
[0675] The p-toluenesulfonic acid is used as a catalyst in the
above example. Boron trifluoride could also be used as a catalyst,
as could a number of other Lewis acids or non-oxidizing acids known
in the art. Other solvents can also be used, for example, benzene,
toluene, chloroform, dichloromethane, etc.
[0676] In other embodiments, anhydrous MgSO.sub.4 or another
suitable agents such as Na.sub.2SO.sub.4, CaSO.sub.4, and
CaCl.sub.2, known in the art is used in place of the
MgSO.sub.4.
Example 4: Exemplary Methods
[0677] In alternative embodiments, the "extract containing a
cannabinoid or a cannabinoid acid" is dissolved in a gradient
elution solution and subjected to a chromatographic purification
step to produce substantially pure cannabinoids and a product
enriched in a given cannabinoid. The purpose of this step is to
first remove the targeted non-cannabinoid material comprising for
example terpenes, carotenes, and flavonoids and also to provide a
degree of separation/fractionation of the various
cannabinoid/cannabinoid acid components of the extract.
[0678] In alternative embodiments, the product of the
chromatographic step is collected in multiple fractions, which can
then be tested for the presence of the desired
cannabinoid/cannabinoid acid using any suitable analytical
technique. Fractions enriched in the desired cannabinoid and/or
cannabinoid acid can then be selected for further purification.
Optionally the gradient elution solution is removed from the
selected fractions or optionally additional gradient solvent is
added to change the polarity or hydrogen potential of the gradient
solvent contained in the selected fraction. The selected fraction
can then be subjected to one or more additional chromatographic
steps and one or more gradient elution solutions modifications.
[0679] In alternative embodiments, the chromatographic step
comprises a column chromatography in a fixed or a continuous mode,
and is can be based on molecular sizing, polarity and/or hydrogen
potentiality. In alternative embodiments, the column matrix
materials are, for example; silica (or silica gel) and alumina
normal phase and reverse phase such as for example C18, C8, C4, C2,
Amino, Cyano, Phenyl, Diol, WAX, SAX, WCX, SCX, Thiol; acidic,
basic and neutral, styrenic, brominated styrenic; ion exchange
resins such as strongly acidic, typically featuring sulfonic acid
groups, e.g. sodium polystyrene sulfonate or polyAMPS; strongly
basic, typically featuring quaternary amino groups, for example,
trimethylammonium groups, e.g. polyAPTAC); weakly acidic, typically
featuring carboxylic acid groups; weakly basic, typically featuring
primary, secondary, and/or tertiary amino groups, e.g. polyethylene
amine.
[0680] In alternative embodiments, various different elution
solutions can be used in combination with this type of matrix, for
example dimethyl sulfoxide, water, dimethylformamide, methanol,
ethylene dichloride, chloroform, propanol, ethanol, isobutanol,
formamide, methylene dichloride, butanol, isopropanol,
tetrahydrofuran, dioxane, hexane, butane, pentane, heptane, octane,
carbon tetrachloride, etc.
[0681] In alternative embodiments, the chromatographic step
comprises column chromatography on for example, a silica (or silica
gel) or an alumina column, and the step can comprise eluting with a
5:1; 4:1; 3:1; 2:1; 1.9:1; 1.8:1; 1.7:1; 1.6:1; 1.5:1; 1.4:1;
1.3:1; 1.2:1; 1.1:1; 1.01:1; 1:1; 0.9:1; 0.5:1; 0.1:1 mixture of
elution solutions such as chloroform, methylene dichloride,
ethylene dichloride, methanol, ethanol, propanol and/or water. Any
suitable combination of normal phase column packing material and
solvent having separation characteristics suitable for use in
separation (fractionation) of cannabinoids and/or cannabinoid acids
can be used with equivalent effect.
[0682] In alternative embodiments, the column gradient eluate is
collected in several fractions. The fractions are tested for the
presence of the desired cannabinoid and/or cannabinoid acid using a
suitable analytical technique, and those fractions containing the
highest amounts of the desired cannabinoid and/or cannabinoid acid
selected for further processing. Solvent can be then removed from
the selected fractions, e.g., by evaporation, e.g., rotary
evaporation or equivalents.
[0683] In alternative embodiments, the fractions enriched in a
given cannabinoid obtained from the gradient elution normal phase
chromatographic step are mixed with a solvent of the opposite
polarity thereby causing a concentration of the cannabinoids in the
second solvent phase. In alternative embodiments, the solvent is
then removed.
[0684] In alternative embodiments, the fractions containing a
product enriched in a given cannabinoid obtained from the gradient
elution normal phase chromatographic step or steps are re-dissolved
in a gradient elution solution. In alternative embodiments, the
fractions containing a product enriched in a given cannabinoid such
as mixtures comprising CBD and THC, or mixtures of CBD and CBG or
mixtures of CBG and CBC, are dissolved in a gradient elution
solution and subjected to a reverse phase chromatographic
purification step to produce substantially pure extracts.
[0685] In alternative embodiments, the product of the reverse phase
chromatographic step is collected in multiple fractions, which can
then be tested for the presence of the desired cannabinoid and/or
cannabinoid acid using any suitable analytical technique. Fractions
enriched in the desired cannabinoid or cannabinoid acid can then be
selected for further purification. In alternative embodiments, the
gradient elution solution is removed from the selected fractions or
optionally additional gradient solvent is added to change the
polarity of the gradient solvent contained in the selected
fraction.
[0686] An exemplary embodiment comprises: fractions enriched in a
given cannabinoid obtained from the gradient elution reverse phase
chromatographic step are mixed with a solvent of the opposite
polarity thereby causing a concentration of the cannabinoids in the
second solvent phase. In alternative embodiments, the solvent is
then removed. In alternative embodiments, the selected fraction is
then subjected to one or more additional reverse phase
chromatographic steps and one or more gradient elution solutions
modifications. In alternative embodiments, the reverse phase
chromatographic step comprises a column chromatography in fixed or
continuous mode, and is can be based on polarity. Examples of
reverse phase column chromatography include but are not limited to
pure silica, alkyl chain-bonded silica, cyano-bonded silica, and
phenyl-bonded silica.
[0687] In alternative embodiments, various different elution
solutions can be used in combination with this type of matrix, for
example dimethyl sulfoxide, water, dimethylformamide, methanol,
saline chloroform, propanol, ethanol, isobutanol, formamide,
butanol, isopropanol, tetrahydrofuran, dioxane, dichloromethane,
dichloroethane, etc.
[0688] In alternative embodiments, the chromatographic step
comprises column chromatography using an ion exchange resin such as
but not limited to RediSep.RTM. Rf SAX, Dowex.RTM. Marathon C
Na.TM., Marathon C H.TM., Marathon MSC-1.TM. or Dowex Marathon
WBA.TM. column, and eluting can be with a 20:1; 15:1; 10:1, 5:1;
4.5:1; 4:1; 3.5:1; 3:1 2:1; 1.9:1; 1.8:1; 1.7:1; 1.6:1; 1.5:1;
1.4:1; 1.3:1; 1.2:1; 1.1:1; 1.01:1; 1.001:1; 0.1:1 mixture of
elution solutions such as chloroform, dichloromethane,
dichloroethane methanol, ethanol, propanol, dimethyl sulfoxide,
pyridine water, dimethylformamide, methanol, ethylene dichloride,
chloroform, propanol, ethanol, isobutanol, formamide, butanol,
isopropanol, tetrahydrofuran, dioxane, dichloromethane,
dichloroethane, acetic acid, carbonic acid, glycolic acid, benzoic
acid, formic acid, oxalic acid, sodium hydroxide, potassium
hydroxide, ammonium hydroxide, ammonium carbonate, etc, and/or
water. Any suitable combination of ion exchange phase column
packing material and solvent having separation characteristics
suitable for use in purification of cannabinoids and cannabinoids
acid can be used with equivalent effect.
[0689] In alternative embodiments, the column gradient eluate is
collected in several fractions. In alternative embodiments, the
fractions are tested for the presence of the desired cannabinoid or
cannabinoid acid using a suitable analytical technique, and those
fractions containing the highest amounts of the desired cannabinoid
or cannabinoid acid. In alternative embodiments, solvent is then
removed from the selected fractions, optionally by evaporation,
e.g., rotary evaporation.
[0690] In alternative embodiments, the fractions containing a
product are enriched in a given cannabinoid acid obtained from the
gradient elution chromatographic step or steps, and the fractions
containing a product enriched in a given cannabinoid acid obtained
from gradient elution reverse phase step or steps are re-dissolved
in a gradient elution solution. In alternative embodiments, the
fractions containing a product enriched in a given cannabinoid acid
such as tetrahydrocannabinolic acid (THCA), are dissolved in a
gradient elution solution and subjected to an ion exchange
chromatographic purification step to produce substantially pure
extracts. In alternative embodiments, the product of the ion
exchange chromatographic step is collected in multiple fractions,
which can then be tested for the presence of the desired
cannabinoid or cannabinoid acid using any suitable analytical
technique. Fractions enriched in the desired cannabinoid or
cannabinoid acid can then be selected for further purification. In
alternative embodiments, the gradient elution solution is removed
from the selected fractions or optionally additional gradient
solvent is added to change the polarity and/or hydrogen potential
of the gradient solvent contained in the selected fraction. An
exemplary embodiment comprises: the fractions enriched in a given
cannabinoid obtained from the gradient elution ion exchange
chromatographic step are mixed with a solvent of the opposite
polarity thereby causing a concentration of the cannabinoids in the
second solvent phase. The solvent can then be removed.
[0691] In alternative embodiments, the selected fraction is then
subjected to one or more additional ion exchange chromatographic
steps and one or more gradient elution solutions modifications. The
ion exchange chromatographic step can comprise column
chromatography in fixed or continuous mode, and can be based on
molecular sizing and polarity ion exchange resin such as but not
limited to RediSep.RTM. Rf SAX, Dowex.RTM. Marathon C Na, Marathon
C H, Marathon MSC-1 or Dowex Marathon WBA.TM..
[0692] In alternative embodiments, various different elution
solutions can be used in combination with this type of matrix, for
example dimethyl sulfoxide, water, dimethylformamide, methanol,
ethylene dichloride, propanol, ethanol, isobutanol, formamide,
methylene dichloride, butanol, isopropanol, tetrahydrofuran,
dioxane, chloroform, acetic acid, carbonic acid, glycolic acid,
benzoic acid, formic acid, oxalic acid, sodium hydroxide, potassium
hydroxide, ammonium hydroxide, ammonium carbonate, etc.
[0693] In alternative embodiments, essential features of the
process are the same for purification of all cannabinoids and
cannabinoid acids. Cannabis plants generally contain complex
mixtures of cannabinoid acids and cannabinoids, although depending
on the variety of cannabis one type of cannabinoid may
pre-dominate. The purpose of the gradient elution chromatographic
steps (ii) is to separate the various cannabinoid and/or
cannabinoid/cannabinoid acid components of the crude plant extract
loaded in step (i), as described above, into substantially pure
fractions or substantially pure mixtures of fractions which are
then optionally subjected to isomerization reactions and/or
elimination reactions and/or additional gradient elution
chromatography comprising normal phase, reverse phase and/or ion
exchange.
[0694] In alternative embodiments, the product of the
chromatographic step is collected in multiple fractions, which can
then be tested for the presence of the desired
cannabinoid/cannabinoid acid using any suitable analytical
technique. Fractions enriched in the desired
cannabinoid/cannabinoid acid can then be selected for further
purification. Hence, the same simple process steps can be adapted
for purification of essentially any plant-derived cannabinoid or
cannabinoid acid. Selectivity for different cannabinoids or
cannabinoid acids can be enhanced by selection of appropriate
starting plant material. By way of example, if it is desired to
prepare substantially pure .DELTA..sup.9 THC or .DELTA..sup.9 THCA
then "high THC" cannabis plants can be selected as the starting
material. Alternatively, if it is desired to prepare substantially
pure CBD or CBDA then "high CBD" cannabis plants can be selected as
the starting material. It is to be understood that processes as
provided herein are of general utility and are not limited to the
use of particular Cannabis varieties as the starting material. The
precise cannabinoid content of any particular Cannabis plant
material can be qualitatively and quantitatively determined using
analytical techniques well known to those skilled in the art, such
as thin-layer chromatography or high-performance liquid
chromatography (HPLC). Thus, one may screen a range of various
Cannabis plants and select those having a high content of the
desired cannabinoid acid or cannabinoid for use as starting
material in a process as provided herein.
[0695] With the use of conventional selective breeding techniques
it is possible to develop cannabis varieties (chemovars) having
varying cannabinoid content. Select cannabis varieties (chemovars)
have relatively high content of CBD, or of the minor cannabinoids
.DELTA..sup.9-tetrahydrocannabivarin (.DELTA..sup.9-THCV),
cannabigerol (CBG) or cannabichromene (CBC). General protocols for
growing of medicinal cannabis and for testing the cannabinoid
content of cannabis plants are described in International patent
application WO 02/064109.
[0696] In alternative embodiments, methods further provide for the
generation of a substantially pure preparation of .DELTA..sup.9 THC
having a chromatographic purity of greater than about 75%, 80%,
90%, 95%, 96%, 97%, 98%, or 99% or more by area normalization of an
HPLC profile. The preparation can be a semi-solid at room
temperature. The preparation can comprises less than about 1.5%,
less than about 0.4%, or less than about 0.2%; or, less than about
0.1% CBD (w/w), less than about 0.5%, less than about 0.4%, or less
than about 0.2%, or less than about 0.1% CBD (w/w) as analyzed by
HPLC.
[0697] In alternative embodiments, the pure .DELTA..sup.9-THC
provided by exemplary methods as provided herein have utility as an
active pharmaceutical agent, and is also useful as a
chromatographic standard, particularly as a comparative standard in
the qualitative analysis of botanical drug substances derived from
cannabis. The availability of highly pure .DELTA..sup.9-THC will
also facilitate studies of the pharmacology of
.DELTA..sup.9-THC.
[0698] In alternative embodiments, an exemplary method for
preparation of substantially pure .DELTA..sup.9 THC comprises:
[0699] i) obtaining an ethanolic solution of a botanical drug
substance from cannabis or hemp plant material, [0700] ii) passing
the solution obtained in step i) through a filter, and collecting
the eluate, [0701] iii) optionally substantially remove solvent
from the eluate, e.g., by evaporation, e.g., rotary evaporation, to
give a cannabinoid enriched fraction, [0702] iv) optionally adding
solvent to the eluate, [0703] v) passing a solution of the
resulting cannabinoid enriched extract through a column packed with
a stationary phase resin such as DOWEX.RTM. MARATHON C Na.TM.,
MARATHON C H.TM., and conducting a gradient elution of 50:1; 20:1;
10:1; 9:1; 8:1; 7:1; 6:1; 5:1; 4:1; 3:1; 2:1; 1;1; 0.5:1; 0.005:1;
0.0005:1 chloroform/methanol or ethanol, [0704] vi) adjusting
hydrogen potential of said gradient solvent solution, [0705] vii)
collecting .DELTA..sup.9-THC and CBD enriched fractions and
optionally removing solvent, e.g., by evaporation, e.g., rotary
evaporation, [0706] viii) mixing the collected .DELTA..sup.9 THC
and CBD with a reaction solvent and a catalyst for a period of time
and adding a neutralizing agent and removing catalyst and
neutralizing agent by filtration to generate a CBD reduced
.DELTA..sup.8-THC and .DELTA..sup.9-THC mixture, optionally
removing reaction solvent, [0707] ix) mixing CBD reduced
.DELTA..sup.8-THC and .DELTA..sup.9-THC mixture with optionally
additional reaction solvent and a stabilizing agent, mixing
stabilizing agent for a period of time, [0708] x) mixing CBD
reduced .DELTA..sup.8-THC and .DELTA..sup.9-THC and stabilizing
agent mixture with an elimination agent. [0709] xi) separation of
elimination agent and stabilizing agent from substantially
.DELTA..sup.9 THC, [0710] xii) optionally re-dissolving the crude
.DELTA..sup.9 THC and CBD prepared in step xi) and passing the
solution through a column packed with reverse phase resin, and
conducting a gradient elution of 10:1; 9:1; 8:1; 7:1; 6:1; 5:1;
4:1; 3:1; 2:1; 1;1 methanol/water, [0711] xiii) optionally
re-dissolving the crude .DELTA..sup.9 THC and CBD prepared in step
xi) such as DOWEX.RTM. MARATHON C Na.TM., MARATHON C H.TM., and
conducting a gradient elution of 50:1; 20:1; 10:1; 9:1; 8:1; 7:1;
6:1; 5:1; 4:1; 3:1; 2:1; 1;1; 0.5:1; 0.005: 1; 0.0005:1 chloroform
and polar solvent mixture. [0712] vi) adjusting hydrogen potential
of said gradient solvent solution [0713] vii) collecting the
.DELTA..sup.9 THC enriched fractions and removing solvent, e.g., by
evaporation, e.g., rotary evaporation, to give a semi-solid
preparation of .DELTA..sup.9 THC. [0714] (viii) collecting the CBD
enriched fractions and removing solvent, e.g., by evaporation,
e.g., rotary evaporation, to give a semi-solid preparation of
CBD.
Example 5: Conversion of CBD to .DELTA..sup.8-THC and
.DELTA..sup.9-THC
[0715] This Example describes an exemplary method for the
conversion of CBD to .DELTA..sup.8-THC and .DELTA..sup.9-THC.
[0716] CBD (1 g) was added to 6.4 ml of reaction solvent and 1%
p-toluenesulfonic acid. In this example, the mixture was reacted
for 48 hours, although other time periods can also be used, as
discussed below. It was then diluted with ether (20 ml) and poured
into water, The upper layer was separated, washed with aqueous 5%
NaHCO.sub.3, then with water, dried over MgSO.sub.4 and evaporated.
GC-MS analysis on the crude product, showed the presence of 1.1%
CBD; 18.4% .DELTA..sup.8-THC and 80.0% .DELTA..sup.9-THC. The crude
product was then subjected to column chromatography. In the example
described above, normal phase HPLC separation is used wherein the
column is for example a silica gel and the mobile phase is an
organic solvent mixture introduced as a gradient described in the
invention. In other embodiments, reverse phase HPLC separation is
used.
[0717] The p-toluenesulfonic acid is used as a catalyst in the
above example. It is of note that boron trifluoride could also be
used as a catalyst, as could a number of other Lewis acids or
non-oxidizing acid catalysts. The exact proportion is not essential
to the reaction proceeding. Other solvents can also be used, for
example, benzene, toluene, chloroform, dichloromethane, etc.
[0718] In other embodiments, anhydrous MgSO.sub.4 or another
suitable agents such as Na.sub.2SO.sub.4, CaSO.sub.4, and
CaCl.sub.2, known in the art is used in place of the
MgSO.sub.4.
Example 6: Conversion of .DELTA..sup.8 THC and .DELTA..sup.9 THC,
and Purification of .DELTA..sup.9 THC
[0719] This Example describes an exemplary method for the
conversion of .DELTA..sup.8-THC and .DELTA..sup.9-THC, and the
purification of .DELTA..sup.9 THC.
[0720] A mixture containing 1.1% CBD; 18.4% .DELTA..sup.8-THC and
80.0% .DELTA..sup.9-THC (1 g) was added to 20 mL of reaction
solvent and then 65 mol % protection agent was added to the
solution. The colorless solution was saturated with hydrogen
chloride at 0.degree. C. in a dry apparatus and stirred. The
mixture was washed with water and treated with sodium bicarbonate.
The solvent layer was concentrated and dried under high vacuum. 3
equivalents of elimination agent was then added to the mixture in a
dry solvent and heated up to 65.degree. C. for 15 min. The mixture
was mixed with solvent and washed with water and treated with
sodium bicarbonate. The organic layer was dried over sodium sulfate
and concentrated and dried under high vacuum. GC-MS analysis on the
crude product, showed the presence of 1% CBD; 3% .DELTA..sup.8-THC
and 96% .DELTA..sup.9-THC. The crude product was then subjected to
column chromatography. In the example described above, normal phase
HPLC separation is used wherein the column is for example a silica
gel and the mobile phase is an organic solvent mixture introduced
as a gradient described in the invention. In other embodiments,
reverse phase HPLC separation is used.
[0721] The zinc chloride is used as the protection agent in the
above example. The exact proportion is not essential to the
reaction proceeding. Other solvents can also be used, for example,
benzene, toluene, chloroform, dichloromethane, etc.
[0722] Example 7: Conversion of CBD to .DELTA..sup.8-THC and
.DELTA..sup.9-THC
[0723] This Example describes an exemplary method for the
conversion of CBD to .DELTA..sup.8-THC and .DELTA..sup.9-THC.
[0724] A hemp extract was obtained by extraction of hemp in
ethanol. The hemp extract was composed of CBD, THC, cannabinoids
and other components and subjected to conversion of CBD to THC
without further purification. The hemp extract and 1.about.5 mol %
p-toluenesulfonic acid were sequentially added to 6.4 mL reaction
solvent. In this example, the reaction mixture was stirred at room
temperature for 24 hours, although other time periods can also be
used, as discussed below. At some time intervals, the reaction
mixture was diluted with ether (20 mL) and poured into water. The
upper layer was separated, washed with aqueous 5% NaHCO.sub.3,
dried over MgSO.sub.4 and concentrated on a rotary evaporator.
GC-MS analysis on the crude product showed the conversion of CBD to
.DELTA..sup.8-THC and .DELTA..sup.8-THC, for example, 0% CBD; 98%
.DELTA..sup.9-THC; 2% .DELTA..sup.8-THC after 24 hours in the
presence of 3 mol % p-toluenesulfonic acid. The crude product was
then subjected to column chromatography. In the example described
above, normal phase HPLC separation is used wherein the column is
for example a silica gel and the mobile phase is an organic solvent
mixture introduced as a gradient described in the invention. In
other embodiments, reverse phase HPLC separation is used.
[0725] The p-toluenesulfonic acid is used as a catalyst in the
above example. Boron trifluoride could also be used as a catalyst,
as could a number of other Lewis acids or non-oxidizing acids known
in the art. Other solvents can also be used, for example, benzene,
toluene, chloroform, dichloromethane, etc.
[0726] In other embodiments, anhydrous MgSO.sub.4 or another
suitable agents such as Na.sub.2SO.sub.4, CaSO.sub.4, and
CaCl.sub.2, known in the art is used in place of the
MgSO.sub.4.
Example 8: Exemplary Methods
[0727] In alternative embodiments, the "extract containing a
cannabinoid or a cannabinoid acid" is dissolved in a gradient
elution solution and subjected to a chromatographic purification
step to produce substantially pure cannabinoids and a product
enriched in a given cannabinoid. The purpose of this step is to
first remove the targeted non-cannabinoid material comprising for
example terpenes, carotenes, and flavonoids and also to provide a
degree of separation/fractionation of the various
cannabinoid/cannabinoid acid components of the extract.
[0728] In alternative embodiments, the product of the
chromatographic step is collected in multiple fractions, which may
then be tested for the presence of the desired
cannabinoid/cannabinoid acid using any suitable analytical
technique. Fractions enriched in the desired cannabinoid and/or
cannabinoid acid can then be selected for further purification.
Optionally the gradient elution solution is removed from the
selected fractions or optionally additional gradient solvent is
added to change the polarity or hydrogen potential of the gradient
solvent contained in the selected fraction. The selected fraction
can then be subjected to one or more additional continuous
chromatographic steps and one or more gradient elution solutions
modifications.
[0729] In alternative embodiments, the chromatographic step
comprises a column chromatography in a fixed or a continuous mode,
and is can be based on molecular sizing, polarity and/or hydrogen
potentiality. In alternative embodiments, the column matrix
materials are, for example; silica (or silica gel) and alumina
normal phase and reverse phase such as for example C18, C8, C4, C2,
Amino, Cyano, Phenyl, Diol, WAX, SAX, WCX, SCX, Thiol; acidic,
basic and neutral, styrenic, brominated styrenic; ion exchange
resins such as strongly acidic, typically featuring sulfonic acid
groups, e.g. sodium polystyrene sulfonate or polyAMPS; strongly
basic, typically featuring quaternary amino groups, for example,
trimethylammonium groups, e.g. polyAPTAC); weakly acidic, typically
featuring carboxylic acid groups; weakly basic, typically featuring
primary, secondary, and/or tertiary amino groups, e.g. polyethylene
amine.
[0730] In alternative embodiments, various different elution
solutions can be used in combination with this type of matrix, for
example dimethyl sulfoxide, pyridine water, dimethylformamide,
methanol, ethylene dichloride, chloroform, propanol, ethanol,
isobutanol, formamide, methylene dichloride, butanol, isopropanol,
tetrahydrofuran, dioxane, hexane, butane, pentane, heptane, octane,
carbon tetrachloride, etc.
[0731] In alternative embodiments, the chromatographic step
comprises column chromatography on for example, a silica (or silica
gel) or an alumina column, and the step can comprise eluting with a
5:1; 4:1; 3:1; 2:1; 1.9:1; 1.8:1; 1.7:1; 1.6:1; 1.5:1; 1.4:1;
1.3:1; 1.2:1; 1.1:1; 1.01:1; 1:1; 0.9:1; 0.5:1; 0.1:1 mixture of
elution solutions such as chloroform, methylene dichloride,
ethylene dichloride, methanol, ethanol, propanol and/or water. Any
suitable combination of normal phase column packing material and
solvent having separation characteristics suitable for use in
separation (fractionation) of cannabinoids and/or cannabinoid acids
can be used with equivalent effect.
[0732] In alternative embodiments, the column gradient eluate is
collected in several fractions. The fractions are tested for the
presence of the desired cannabinoid and/or cannabinoid acid using a
suitable analytical technique, and those fractions containing the
highest amounts of the desired cannabinoid and/or cannabinoid acid
selected for further processing. Solvent can be then removed from
the selected fractions, e.g., by evaporation, e.g., rotary
evaporation or equivalents.
[0733] In alternative embodiments, the fractions enriched in a
given cannabinoid obtained from the gradient elution normal phase
chromatographic step are mixed with a solvent of the opposite
polarity thereby causing a concentration of the cannabinoids in the
second solvent phase. In alternative embodiments, the solvent is
then removed.
[0734] In alternative embodiments, the fractions containing a
product enriched in a given cannabinoid obtained from the gradient
elution normal phase chromatographic step or steps are re-dissolved
in a gradient elution solution. In alternative embodiments, the
fractions containing a product enriched in a given cannabinoid such
as mixtures comprising CBD and THC, or mixtures of CBD and CBG or
mixtures of CBG and CBC, are dissolved in a gradient elution
solution and subjected to a reverse phase chromatographic
purification step to produce substantially pure extracts.
[0735] In alternative embodiments, the product of the reverse phase
chromatographic step is collected in multiple fractions, which can
then be tested for the presence of the desired cannabinoid and/or
cannabinoid acid using any suitable analytical technique. Fractions
enriched in the desired cannabinoid or cannabinoid acid can then be
selected for further purification. In alternative embodiments, the
gradient elution solution is removed from the selected fractions or
optionally additional gradient solvent is added to change the
polarity of the gradient solvent contained in the selected
fraction.
[0736] An exemplary embodiment comprises: fractions enriched in a
given cannabinoid obtained from the gradient elution reverse phase
chromatographic step are mixed with a solvent of the opposite
polarity thereby causing a concentration of the cannabinoids in the
second solvent phase. In alternative embodiments, the solvent is
then removed. In alternative embodiments, the selected fraction is
then subjected to one or more additional reverse phase
chromatographic steps and one or more gradient elution solutions
modifications. In alternative embodiments, the reverse phase
chromatographic step comprises a column chromatography in fixed or
continuous mode, and is can be based on polarity. Examples of
reverse phase column chromatography include but are not limited to
pure silica, alkyl chain-bonded silica, cyano-bonded silica, and
phenyl-bonded silica.
[0737] In alternative embodiments, various different elution
solutions can be used in combination with this type of matrix, for
example dimethyl sulfoxide, pyridine water, dimethylformamide,
methanol, saline chloroform, propanol, ethanol, isobutanol,
formamide, butanol, isopropanol, tetrahydrofuran, dioxane,
dichloromethane, di chloroethane, etc.
[0738] In alternative embodiments, the chromatographic step
comprises column chromatography using an ion exchange resin such as
but not limited to RediSep.RTM. Rf SAX, Dowex.RTM. Marathon C Na,
Marathon C H, Marathon MSC-1 or Dowex Marathon WBATM column, and
eluting can be with a 20:1; 15:1; 10:1, 5:1; 4.5:1; 4:1; 3.5:1; 3:1
2:1; 1.9:1; 1.8:1; 1.7:1; 1.6:1; 1.5:1; 1.4:1; 1.3:1; 1.2:1; 1.1:1;
1.01:1; 1.001:1; 0.1:1 mixture of elution solutions such as
chloroform, dichloromethane, dichloroethane, methanol, ethanol,
propanol, dimethyl sulfoxide, pyridine water, dimethylformamide,
methanol, ethylene dichloride, chloroform, propanol, ethanol,
isobutanol, formamide, butanol, isopropanol, tetrahydrofuran,
dioxane, chloroform dichloromethane, dichloroethane, acetic acid,
carbonic acid, glycolic acid, benzoic acid, formic acid, oxalic
acid, sodium hydroxide, potassium hydroxide, ammonium hydroxide,
ammonium carbonate, etc, and/or water. Any suitable combination of
ion exchange phase column packing material and solvent having
separation characteristics suitable for use in purification of
cannabinoids and cannabinoids acid can be used with equivalent
effect.
[0739] In alternative embodiments, the column gradient eluate is
collected in several fractions. In alternative embodiments, the
fractions are tested for the presence of the desired cannabinoid or
cannabinoid acid using a suitable analytical technique, and those
fractions containing the highest amounts of the desired cannabinoid
or cannabinoid acid. In alternative embodiments, solvent is then
removed from the selected fractions, optionally by evaporation,
e.g., rotary evaporation.
[0740] In alternative embodiments, the fractions containing a
product are enriched in a given cannabinoid acid obtained from the
gradient elution chromatographic step or steps, and the fractions
containing a product enriched in a given cannabinoid acid obtained
from gradient elution reverse phase step or steps are re-dissolved
in a gradient elution solution. In alternative embodiments, the
fractions containing a product enriched in a given cannabinoid acid
such as tetrahydrocannabinolic acid (THCA), are dissolved in a
gradient elution solution and subjected to an ion exchange
chromatographic purification step to produce substantially pure
extracts. In alternative embodiments, the product of the ion
exchange chromatographic step is collected in multiple fractions,
which can then be tested for the presence of the desired
cannabinoid or cannabinoid acid using any suitable analytical
technique. Fractions enriched in the desired cannabinoid or
cannabinoid acid can then be selected for further purification. In
alternative embodiments, the gradient elution solution is removed
from the selected fractions or optionally additional gradient
solvent is added to change the polarity and/or hydrogen potential
of the gradient solvent contained in the selected fraction. An
exemplary embodiment comprises: the fractions enriched in a given
cannabinoid obtained from the gradient elution ion exchange
chromatographic step are mixed with a solvent of the opposite
polarity thereby causing a concentration of the cannabinoids in the
second solvent phase. The solvent can then be removed.
[0741] In alternative embodiments, the selected fraction is then
subjected to one or more additional ion exchange chromatographic
steps and one or more gradient elution solutions modifications. The
ion exchange chromatographic step can comprise column
chromatography in fixed or continuous mode, and can be based on
molecular sizing and polarity ion exchange resin such as but not
limited to RediSep.RTM. Rf SAX, Dowex.RTM. Marathon C Na, Marathon
C H, Marathon MSC-1 or Dowex Marathon WBA.TM..
[0742] In alternative embodiments, various different elution
solutions can be used in combination with this type of matrix, for
example dimethyl sulfoxide, pyridine water, dimethylformamide,
methanol, ethylene dichloride, chloroform, propanol, ethanol,
isobutanol, formamide, methylene dichloride, butanol, isopropanol,
tetrahydrofuran, dioxane, acetic acid, carbonic acid, glycolic
acid, benzoic acid, formic acid, oxalic acid, sodium hydroxide,
potassium hydroxide, ammonium hydroxide, ammonium carbonate,
etc.
[0743] In alternative embodiments, essential features of the
process are the same for purification of all cannabinoids and
cannabinoid acids. Cannabis plants generally contain complex
mixtures of cannabinoid acids and cannabinoids, although depending
on the variety of cannabis one type of cannabinoid may
pre-dominate. The purpose of the gradient elution chromatographic
steps (ii) is to separate the various cannabinoid and/or
cannabinoid/cannabinoid acid components of the crude plant extract
loaded in step (i), as described above, into substantially pure
fractions or substantially pure mixtures of fractions which are
then optionally subjected to isomerization reactions and/or
elimination reactions and/or additional gradient elution
chromatography comprising normal phase, reverse phase and/or ion
exchange.
[0744] In alternative embodiments, the product of the
chromatographic step is collected in multiple fractions, which can
then be tested for the presence of the desired
cannabinoid/cannabinoid acid using any suitable analytical
technique. Fractions enriched in the desired
cannabinoid/cannabinoid acid can then be selected for further
purification. Hence, the same simple process steps can be adapted
for purification of essentially any plant-derived cannabinoid or
cannabinoid acid. Selectivity for different cannabinoids or
cannabinoid acids can be enhanced by selection of appropriate
starting plant material. By way of example, if it is desired to
prepare substantially pure .DELTA..sup.8 THC then high CBD and/or
high .DELTA..sup.9 THC and/or .DELTA..sup.9THCA cannabis plants can
be selected as the starting material. Alternatively, if it is
desired to prepare substantially pure CBD or CBDA then "high CBD"
cannabis plants can be selected as the starting material. I is to
be understood that processes as provided herein are of general
utility and are not limited to the use of particular Cannabis
varieties as the starting material. The precise cannabinoid content
of any particular Cannabis plant material can be qualitatively and
quantitatively determined using analytical techniques well known to
those skilled in the art, such as thin-layer chromatography or
high-performance liquid chromatography (HPLC). Thus, one can screen
a range of various Cannabis plants and select those having a high
content of the desired cannabinoid acid or cannabinoid for use as
starting material in a process as provided herein.
[0745] With the use of conventional selective breeding techniques
it is possible to develop cannabis varieties (chemovars) having
varying cannabinoid content. Select cannabis varieties (chemovars)
have relatively high content of CBD, or of the minor cannabinoids
.DELTA..sup.9-tetrahydrocannabivarin (.DELTA..sup.9-THCV),
cannabigerol (CBG) or cannabichromene (CBC). General protocols for
growing of medicinal cannabis and for testing the cannabinoid
content of cannabis plants are described in International patent
application WO 02/064109.
[0746] In alternative embodiments, methods further provide for the
generation of a substantially pure preparation of .DELTA..sup.8 THC
having a chromatographic purity of greater than about 75%, 80%,
90%, 95%, 96%, 97%, 98%, or 99% or more by area normalization of an
HPLC profile. The preparation can be a semi-solid at room
temperature. The preparation can comprises less than about 1.5%,
less than about 0.4%, or less than about 0.2%; or, less than about
0.1% CBD (w/w), less than about 0.5%, less than about 0.4%, or less
than about 0.2%, or less than about 0.1% CBD (w/w) as analyzed by
HPLC.
[0747] In alternative embodiments, the pure .DELTA..sup.8-THC
provided by exemplary methods as provided herein have utility as an
active pharmaceutical agent, and is also useful as a
chromatographic standard, particularly as a comparative standard in
the qualitative analysis of botanical drug substances derived from
cannabis. The availability of highly pure .DELTA..sup.8-THC will
also facilitate studies of the pharmacology of
.DELTA..sup.8-THC.
[0748] In alternative embodiments, an exemplary method for
preparation of substantially pure .DELTA..sup.9 THC comprises:
[0749] i) obtaining an ethanolic solution of a botanical drug
substance from cannabis or hemp plant material, [0750] ii) passing
the solution obtained in step i) through a filter, and collecting
the eluate, [0751] iii) optionally substantially remove solvent
from the eluate, e.g., by evaporation, e.g., rotary evaporation, to
give a cannabinoid enriched fraction, [0752] iv) optionally adding
solvent to the eluate, [0753] v) passing a solution of the
resulting cannabinoid enriched extract through a column packed with
a stationary phase resin such as DOWEX.RTM. MARATHON C Na.TM.,
MARATHON C H.TM., and conducting a gradient elution of 50:1; 20:1;
10:1; 9:1; 8:1; 7:1; 6:1; 5:1; 4:1; 3:1; 2:1; 1; 1; 0.5:1; 0.005:1;
0.0005:1 chloroform/methanol or ethanol, [0754] vi) adjusting
hydrogen potential of said gradient solvent solution, [0755] vii)
collecting .DELTA..sup.8-THC, .DELTA..sup.9-THC and CBD enriched
fractions and optionally removing solvent, e.g., by evaporation,
e.g., rotary evaporation, [0756] viii) mixing the collected
.DELTA..sup.8-THC, .DELTA..sup.9 THC and CBD with a reaction
solvent and a catalyst for a period of time and adding a
neutralizing agent and removing catalyst and neutralizing agent by
filtration to generate a substantially pure .DELTA..sup.8-THC
product with a reduced CBD and .DELTA..sup.9-THC content,
optionally removing reaction solvent, [0757] viii) optionally
re-dissolving the substantially .DELTA..sup.8 THC mixture prepared
in step viii) and passing the solution through a column packed with
reverse phase resin, and conducting a gradient elution of 10:1;
9:1; 8:1; 7:1; 6:1; 5:1; 4:1; 3:1; 2:1; 1;1 methanol/water, [0758]
x) optionally re-dissolving the substantially .DELTA..sup.8 THC
prepared in step viii) such as DOWEX.RTM. MARATHON C Na.TM.,
MARATHON C H.TM., and conducting a gradient elution of 50:1; 20:1;
10:1; 9:1; 8:1; 7:1; 6:1; 5:1; 4:1; 3:1; 2:1; 1;1; 0.5:1; 0.005: 1;
0.0005:1 chloroform and polar solvent mixture. [0759] xi) adjusting
hydrogen potential of said gradient solvent solution [0760] xii)
collecting the .DELTA..sup.8 THC enriched fractions and removing
solvent, e.g., by evaporation, e.g., rotary evaporation to give a
semi-solid preparation of .DELTA..sup.8 THC.
Example 9: Conversion of CBD to .DELTA..sup.8-THC and
.DELTA..sup.9-THC
[0761] This Example describes an exemplary method for the
conversion of CBD to .DELTA..sup.8-THC.
[0762] CBD (15 g) was added to 240 ml of reaction solvent and
p-toluenesulfonic acid. In this example, the mixture was reacted
for 27 hours, although other time periods can also be used, as
discussed below. It was then diluted with ether (20 ml) and poured
into water. The upper layer was separated, washed with aqueous 5%
NaHCO.sub.3, then with water, dried over MgSO.sub.4 and evaporated.
GC-MS analysis on the crude product, showed the presence of 96.5%
.DELTA..sup.8-THC and 2.5% .DELTA..sup.9-THC. The crude product was
then subjected to column chromatography. In the example described
above, normal phase HPLC separation is used wherein the column is
for example a silica gel and the mobile phase is an organic solvent
mixture introduced as a gradient described in the invention. In
other embodiments, reverse phase HPLC separation is used.
[0763] The p-toluenesulfonic acid is used as a catalyst in the
above example. It is of note that boron trifluoride could also be
used as a catalyst, as could a number of other Lewis acids or
non-oxidizing acid catalysts. The exact proportion is not essential
to the reaction proceeding. Other solvents can also be used, for
example, benzene, toluene, chloroform, dichloromethane, etc.
[0764] In other embodiments, anhydrous MgSO.sub.4 or another
suitable agents such as Na.sub.2SO.sub.4, CaSO.sub.4, and
CaCl.sub.2, known in the art is used in place of the
MgSO.sub.4.
Example 10: Exemplary Methods
[0765] In alternative embodiments, the "extract containing a
cannabinoid or a cannabinoid acid" is dissolved in a gradient
elution solution and subjected to one or multiple continuous
chromatographic purification step to produce substantially pure
cannabinoids and a product enriched in a given cannabinoid. The
purpose of this step is to first remove the targeted
non-cannabinoid material comprising for example terpenes,
carotenes, and flavonoids and also to provide a degree of
separation/fractionation of the various cannabinoid/cannabinoid
acid components of the extract.
[0766] In alternative embodiments, the product of one or more
continuous chromatographic steps is collected in multiple
fractions, which may then be tested for the presence of the desired
cannabinoid/cannabinoid acid using any suitable analytical
technique. Fractions enriched in the desired cannabinoid and/or
cannabinoid acid can then be selected for further purification.
Optionally the gradient elution solution is removed from the
selected fractions or optionally additional gradient solvent is
added to change the polarity or hydrogen potential of the gradient
solvent contained in the selected fraction. The selected fraction
can then be subjected to one or more additional chromatographic
steps and one or more gradient elution solutions modifications.
[0767] In alternative embodiments, the chromatographic step
comprises a column chromatography in a continuous mode, and is
based on molecular sizing, polarity and/or hydrogen potentiality.
In alternative embodiments, the column matrix materials are, for
example; silica (or silica gel), alumina and reverse phase for
example C18, C8, C4, C2, Amino, Cyano, Phenyl, Diol, WAX, SAX, WCX,
SCX, Thiol; acidic, basic and neutral, styrenic, brominated
styrenic; ion exchange resins such as strongly acidic, typically
featuring sulfonic acid groups, e.g. sodium polystyrene sulfonate
or polyAMPS; strongly basic, typically featuring quaternary amino
groups, for example, trimethylammonium groups, e.g. polyAPTAC);
weakly acidic, typically featuring carboxylic acid groups; weakly
basic, typically featuring primary, secondary, and/or tertiary
amino groups, e.g. polyethylene amine.
[0768] In alternative embodiments, various different elution
solutions can be used in combination with this type of matrix, for
example dimethyl sulfoxide, pyridine, water, dimethylformamide,
methanol, saline, ethylene dichloride, chloroform, propanol,
ethanol, isobutanol, formamide, methylene dichloride, butanol,
isopropanol, tetrahydrofuran, dioxane, hexane, butane, pentane,
heptane, octane, carbon tetrachloride, etc.
[0769] In alternative embodiments, the chromatographic step
comprises continuous column chromatography on for example, a silica
(or silica gel) or an alumina column, and the step can comprise
eluting with a 5:1; 4:1; 3:1; 2:1; 1.9:1; 1.8:1; 1.7:1; 1.6:1;
1.5:1; 1.4:1; 1.3:1; 1.2:1; 1.1:1; 1.01:1; 1:1; 0.9:1; 0.5:1; 0.1:1
mixture of elution solutions such as chloroform, methylene
dichloride, ethylene dichloride, methanol, ethanol, propanol and/or
water. Any suitable combination of normal phase column packing
material and solvent having separation characteristics suitable for
use in separation (fractionation) of cannabinoids and/or
cannabinoid acids can be used with equivalent effect.
[0770] In alternative embodiments, the column gradient eluate is
collected in several fractions. The fractions are tested for the
presence of the desired cannabinoid and/or cannabinoid acid using a
suitable analytical technique, and those fractions containing the
highest amounts of the desired cannabinoid and/or cannabinoid acid
selected for further processing. Solvent can be then removed from
the selected fractions, e.g., by evaporation, e.g., rotary
evaporation or equivalents.
[0771] In alternative embodiments, the fractions enriched in a
given cannabinoid obtained from the gradient elution normal phase
chromatographic step are mixed with a solvent of the opposite
polarity thereby causing a concentration of the cannabinoids in the
second solvent phase. In alternative embodiments, the solvent is
then removed.
[0772] In alternative embodiments, the fractions containing a
product enriched in a given cannabinoid obtained from the gradient
elution normal phase continuous chromatographic step or steps are
re-dissolved in a gradient elution solution. In alternative
embodiments, the fractions containing a product enriched in a given
cannabinoid such as mixtures comprising CBD and THC, or mixtures of
CBD and CBG or mixtures of CBG and CBC, are dissolved in a gradient
elution solution and subjected to a continuous reverse phase
chromatographic purification step to produce substantially pure
extracts.
[0773] In alternative embodiments, the product of the reverse phase
chromatographic step is collected in multiple fractions, which can
then be tested for the presence of the desired cannabinoid and/or
cannabinoid acid using any suitable analytical technique. Fractions
enriched in the desired cannabinoid or cannabinoid acid can then be
selected for further purification. In alternative embodiments, the
gradient elution solution is removed from the selected fractions or
optionally additional gradient solvent is added to change the
polarity of the gradient solvent contained in the selected
fraction.
[0774] An exemplary embodiment comprises: fractions enriched in a
given cannabinoid obtained from the gradient elution reverse phase
chromatographic step are mixed with a solvent of the opposite
polarity thereby causing a concentration of the cannabinoids in the
second solvent phase. In alternative embodiments, the solvent is
then removed. In alternative embodiments, the selected fraction is
then subjected to one or more additional reverse phase
chromatographic steps and one or more gradient elution solutions
modifications. In alternative embodiments, the reverse phase
chromatographic step comprises a column chromatography continuous
mode, and can be based on polarity or affinity.
[0775] In alternative embodiments, various different elution
solutions can be used in combination with this type of matrix, for
example dimethyl sulfoxide, pyridine water, dimethylformamide,
methanol, saline chloroform, propanol, ethanol, isobutanol,
formamide, butanol, isopropanol, tetrahydrofuran, dioxane,
dichloromethane, dichloroethane, etc.
[0776] In alternative embodiments, the chromatographic step
comprises continuous column chromatography using an ion exchange
resin such as but not limited to RediSep.RTM. Rf SAX, Dowex.RTM.
Marathon C Na, Marathon C H, Marathon MSC-1 or Dowex Marathon WBATM
column, and eluting can be with a 20:1; 15:1; 10:1, 5:1; 4.5:1;
4:1; 3.5:1; 3:1 2:1; 1.9:1; 1.8:1; 1.7:1; 1.6:1; 1.5:1; 1.4:1;
1.3:1; 1.2:1; 1.1:1; 1.01:1; 1.001:1; 0.1:1 mixture of elution
solutions such as chloroform, dichloromethane, dichloroethane
methanol, ethanol, propanol, dimethyl sulfoxide, water,
dimethylformamide, methanol, ethylene dichloride, chloroform,
propanol, ethanol, isobutanol, formamide, butanol, isopropanol,
tetrahydrofuran, dioxane, chloroform dichloromethane,
dichloroethane, acetic acid, carbonic acid, glycolic acid, benzoic,
methanoic, formic acid, oxalic acid, sodium hydroxide, potassium
hydroxide, ammonium hydroxide, ammonium carbonate, etc, and/or
water. Any suitable combination of ion exchange phase column
packing material and solvent having separation characteristics
suitable for use in purification of cannabinoids and cannabinoids
acid can be used with equivalent effect.
[0777] In alternative embodiments, the column gradient eluate is
collected in several fractions. In alternative embodiments, the
fractions are tested for the presence of the desired cannabinoid or
cannabinoid acid using a suitable analytical technique, and those
fractions containing the highest amounts of the desired cannabinoid
or cannabinoid acid. In alternative embodiments, solvent is then
removed from the selected fractions, optionally by evaporation,
e.g., rotary evaporation.
[0778] In alternative embodiments, the fractions containing a
product are enriched in a given cannabinoid acid obtained from the
continuous gradient elution chromatographic step or steps, and the
fractions containing a product enriched in a given cannabinoid acid
obtained from gradient elution reverse phase step or steps are
re-dissolved in a gradient elution solution. In alternative
embodiments, the fractions containing a product enriched in a given
cannabinoid acid such as tetra-hydrocannabinolic acid (THCA), are
dissolved in a gradient elution solution and subjected to a
continuous ion exchange chromatographic purification step to
produce substantially pure extracts. In alternative embodiments,
the product of the ion exchange chromatographic step is collected
in multiple fractions, which can then be tested for the presence of
the desired cannabinoid or cannabinoid acid using any suitable
analytical technique. Fractions enriched in the desired cannabinoid
or cannabinoid acid can then be selected for further purification.
In alternative embodiments, the gradient elution solution is
removed from the selected fractions or optionally additional
gradient solvent is added to change the polarity and/or hydrogen
potential of the gradient solvent contained in the selected
fraction. An exemplary embodiment comprises: the fractions enriched
in a given cannabinoid obtained from the gradient elution ion
exchange chromatographic step are mixed with a solvent of the
opposite polarity thereby causing a concentration of the
cannabinoids in the second solvent phase. The solvent can then be
removed.
[0779] In alternative embodiments, the selected fraction is then
subjected to one or more additional continuous ion exchange
chromatographic steps and one or more gradient elution solutions
modifications. The continuous ion exchange chromatographic step can
comprise column chromatography in fixed or continuous mode, and can
be based on molecular sizing and polarity ion exchange resin such
as but not limited to Redi Sep.RTM. Rf SAX, Dowex.RTM. Marathon C
Na, Marathon C H, Marathon MSC-1 or Dowex Marathon WBA.TM..
[0780] In alternative embodiments, various different elution
solutions can be used in combination with this type of matrix, for
example dimethyl sulfoxide, pyridine, water, dimethylformamide,
methanol, saline, ethylene dichloride, chloroform, propanol,
ethanol, isobutanol, formamide, methylene dichloride, butanol,
isopropanol, tetrahydrofuran, dioxane, chloroform, acetic acid,
carbonic acid, glycolic acid, benzoic acid, formic acid, oxalic
acid, sodium hydroxide, potassium hydroxide, ammonium hydroxide,
ammonium carbonate, etc.
[0781] In alternative embodiments, essential features of the
process are the same for purification of all cannabinoids and
cannabinoid acids. Cannabis plants generally contain complex
mixtures of cannabinoid acids and cannabinoids, although depending
on the variety of cannabis one type of cannabinoid may
pre-dominate. The purpose of the gradient elution chromatographic
steps (ii) is to continuously separate the various cannabinoid
and/or cannabinoid/cannabinoid acid components of the crude plant
extract loaded in step (i), as described above, into substantially
pure fractions or substantially pure mixtures of fractions which
are then optionally subjected to additional gradient elution
chromatography comprising normal phase, reverse phase and/or ion
exchange.
[0782] In alternative embodiments, the product of the
chromatographic step is collected in multiple fractions, which can
then be tested for the presence of the desired
cannabinoid/cannabinoid acid using any suitable analytical
technique. Fractions enriched in the desired
cannabinoid/cannabinoid acid can then be selected for further
purification. Hence, the same simple process steps can be adapted
for purification of essentially any plant-derived cannabinoid or
cannabinoid acid. Selectivity for different cannabinoids or
cannabinoid acids can be enhanced by selection of appropriate
starting plant material. By way of example, if it is desired to
prepare substantially pure .DELTA..sup.9 THC or .DELTA..sup.9 THCA
then "high THC" cannabis plants can be selected as the starting
material. Alternatively, if it is desired to prepare substantially
pure CBD or CBDA then "high CBD" cannabis plants can be selected as
the starting material. It is to be understood that processes are
provided herein are of general utility and are not limited to the
use of particular Cannabis varieties as the starting material. The
precise cannabinoid content of any particular Cannabis plant
material can be qualitatively and quantitatively determined using
analytical techniques well known to those skilled in the art, such
as thin-layer chromatography or high-performance liquid
chromatography (HPLC). Thus, one may screen a range of various
Cannabis plants and select those having a high content of the
desired cannabinoid acid or cannabinoid for use as starting
material in a process as provided herein.
[0783] With the use of conventional selective breeding techniques
it is possible to develop cannabis varieties (chemovars) having
varying cannabinoid content. Select cannabis varieties (chemovars)
have relatively high content of CBD, or of the minor cannabinoids
.DELTA..sup.9-tetrahydrocannabivarin (.DELTA..sup.9-THCV),
cannabigerol (CBG) or cannabichromene (CBC). General protocols for
growing of medicinal cannabis and for testing the cannabinoid
content of cannabis plants are described in International patent
application WO 02/064109.
[0784] In alternative embodiments, methods further provide for the
generation of a substantially pure preparation of .DELTA..sup.9 THC
having a chromatographic purity of greater than about 75%, 80%,
90%, 95%, 96%, 97%, 98%, or 99% or more by area normalization of an
HPLC profile. The preparation can be a semi-solid at room
temperature. The preparation can comprises less than about 1.5%,
less than about 0.4%, or less than about 0.2%; or, less than about
0.1% CBD (w/w), less than about 0.5%, less than about 0.4%, or less
than about 0.2%, or less than about 0.1% CBD (w/w) as analyzed by
HPLC.
[0785] In alternative embodiments, the pure .DELTA..sup.9-THC
provided by exemplary methods as provided herein have utility as an
active pharmaceutical agent, and is also useful as a
chromatographic standard, particularly as a comparative standard in
the qualitative analysis of botanical drug substances derived from
cannabis. The availability of highly pure .DELTA..sup.9-THC will
also facilitate studies of the pharmacology of
.DELTA..sup.9-THC.
[0786] Exemplary method [0787] i) obtaining an ethanolic solution
of a botanical drug substance from cannabis or hemp plant material,
[0788] ii) passing the solution obtained in step i) through a
filter, and collecting the eluate, [0789] iii) optionally
substantially remove solvent from the eluate, e.g., by evaporation,
e.g., rotary evaporation to give a cannabinoid enriched fraction,
[0790] iv) optionally adding solvent to the eluate, [0791] v)
continuously loading a solution of the resulting cannabinoid
enriched extract onto a column packed with a stationary phase resin
such as DOWEX.RTM. MARATHON C Na.TM., MARATHON C H.TM., and
continuously conducting a gradient elution of 50:1;20:1;10:1; 9:1;
8:1; 7:1; 6:1; 5:1; 4:1; 3:1; 2:1; 1;1; 0.5:1; 0.005:1; 0.0005:1
chloroform/methanol or ethanol, [0792] vi) adjusting hydrogen
potential of said gradient solvent solution, [0793] vii) collecting
.DELTA..sup.9-THC and CBD enriched fractions and optionally
removing solvent, e.g., by evaporation, e.g., rotary evaporation,
[0794] viii) optionally re-dissolving the crude .DELTA..sup.9 THC
and CBD prepared in step vii) and continuously loading the solution
on a column packed with a normal phase reverse phase resin, and
continuously conducting a gradient elution of 10:1; 9:1; 8:1; 7:1;
6:1; 5:1; 4:1; 3:1; 2:1; 1;1 methanol/water, [0795] xiii)
optionally re-dissolving the crude .DELTA..sup.9 THC and CBD
prepared in step vii) such as DOWEX.RTM. MARATHON C Na.TM.,
MARATHON C H.TM., and conducting a gradient elution of 50:1; 20:1;
10:1; 9:1; 8:1; 7:1; 6:1; 5:1; 4:1; 3:1; 2:1; 1;1; 0.5:1; 0.005: 1;
0.0005:1 chloroform and polar solvent mixture. [0796] vi) adjusting
hydrogen potential of said gradient solvent solution [0797] vii)
collecting the .DELTA..sup.9 THC enriched fractions and removing
solvent, e.g., by evaporation, e.g., rotary evaporation to give a
semi-solid preparation of .DELTA..sup.9 THC. [0798] (viii)
collecting the CBD enriched fractions and removing solvent, e.g.,
by evaporation, e.g., rotary evaporation to give a semi-solid
preparation of CBD.
Example 11: Exemplary Methods
[0799] In alternative embodiments, the "extract containing a
cannabinoid or a cannabinoid acid" is dissolved in a gradient
elution solution and subjected to a chromatographic purification
step to produce substantially pure cannabinoids and a product
enriched in a given cannabinoid. The purpose of this step is to
first remove the targeted non-cannabinoid material comprising for
example terpenes, carotenes, and flavonoids and also to provide a
degree of separation/fractionation of the various
cannabinoid/cannabinoid acid components of the extract.
[0800] In alternative embodiments, the product of the
chromatographic step is collected in multiple fractions, which can
then be tested for the presence of the desired
cannabinoid/cannabinoid acid using any suitable analytical
technique. Fractions enriched in the desired cannabinoid and/or
cannabinoid acid can then be selected for further purification.
Optionally the gradient elution solution is removed from the
selected fractions or optionally additional gradient solvent is
added to change the polarity or hydrogen potential of the gradient
solvent contained in the selected fraction. The selected fraction
can then be subjected to one or more additional chromatographic
steps and one or more gradient elution solutions modifications.
[0801] In alternative embodiments, the chromatographic step
comprises a column chromatography in a fixed or a continuous mode,
and is can be based on molecular sizing, polarity and/or hydrogen
potentiality. In alternative embodiments, the column matrix
materials are, for example; silica (or silica gel) and alumina
normal phase and reverse phase such as for example C18, C8, C4, C2,
Amino, Cyano, Phenyl, Diol, WAX, SAX, WCX, SCX, Thiol; acidic,
basic and neutral, styrenic, brominated styrenic; ion exchange
resins such as strongly acidic, typically featuring sulfonic acid
groups, e.g. sodium polystyrene sulfonate or polyAMPS; strongly
basic, typically featuring quaternary amino groups, for example,
trimethylammonium groups, e.g. polyAPTAC); weakly acidic, typically
featuring carboxylic acid groups; weakly basic, typically featuring
primary, secondary, and/or tertiary amino groups, e.g. polyethylene
amine.
[0802] In alternative embodiments, various different elution
solutions can be used in combination with this type of matrix, for
example dimethyl sulfoxide, pyridine, water, dimethylformamide,
methanol, saline, ethylene dichloride, chloroform, propanol,
ethanol, isobutanol, formamide, methylene dichloride, butanol,
isopropanol, tetrahydrofuran, dioxane, hexane, butane, pentane,
heptane, octane, carbon tetrachloride, etc.
[0803] In alternative embodiments, the chromatographic step
comprises column chromatography on for example, a silica (or silica
gel) or an alumina column, and the step can comprise eluting with a
5:1; 4:1; 3:1; 2:1; 1.9:1; 1.8:1; 1.7:1; 1.6:1; 1.5:1; 1.4:1;
1.3:1; 1.2:1; 1.1:1; 1.01:1; 1:1; 0.9:1; 0.5:1; 0.1:1 mixture of
elution solutions such as chloroform, methylene dichloride ,
ethylene dichloride, methanol, ethanol, propanol and/or water. Any
suitable combination of normal phase column packing material and
solvent having separation characteristics suitable for use in
separation (fractionation) of cannabinoids and/or cannabinoid acids
can be used with equivalent effect.
[0804] In alternative embodiments, the column gradient eluate is
collected in several fractions. The fractions are tested for the
presence of the desired cannabinoid and/or cannabinoid acid using a
suitable analytical technique, and those fractions containing the
highest amounts of the desired cannabinoid and/or cannabinoid acid
selected for further processing. Solvent can be then removed from
the selected fractions, e.g., by rotary evaporation or
equivalents.
[0805] In alternative embodiments, the fractions enriched in a
given cannabinoid obtained from the gradient elution normal phase
chromatographic step are mixed with a solvent of the opposite
polarity thereby causing a concentration of the cannabinoids in the
second solvent phase. In alternative embodiments, the solvent is
then removed.
[0806] In alternative embodiments, the fractions containing a
product enriched in a given cannabinoid obtained from the gradient
elution normal phase chromatographic step or steps are re-dissolved
in a gradient elution solution. In alternative embodiments, the
fractions containing a product enriched in a given cannabinoid such
as mixtures comprising CBD and THC, or mixtures of CBD and CBG or
mixtures of CBG and CBC, are dissolved in a gradient elution
solution and subjected to a reverse phase chromatographic
purification step to produce substantially pure extracts.
[0807] In alternative embodiments, the product of the reverse phase
chromatographic step is collected in multiple fractions, which can
then be tested for the presence of the desired cannabinoid and/or
cannabinoid acid using any suitable analytical technique. Fractions
enriched in the desired cannabinoid or cannabinoid acid can then be
selected for further purification. In alternative embodiments, the
elution solution is removed from the selected fractions or
optionally additional solvent is added to change the polarity of
the solution contained in the selected fraction.
[0808] An exemplary embodiment comprises: fractions enriched in a
given cannabinoid obtained from the elution of a reverse phase
chromatographic step are mixed with a solvent of the opposite
polarity thereby causing a concentration of the cannabinoids in the
second solvent phase. In alternative embodiments, the solvent is
then removed. In alternative embodiments, the selected fraction is
then subjected to one or more additional reverse phase
chromatographic steps and one or more elution solutions
modifications. In alternative embodiments, the reverse phase
chromatographic step comprises a column chromatography in fixed or
continuous mode, and can be based on polarity. Examples of reverse
phase column chromatography include but are not limited to pure
silica, alkyl chain-bonded silica, cyano-bonded silica, and
phenyl-bonded silica.
[0809] In alternative embodiments, various different elution
solutions can be used in combination with this type of matrix, for
example dimethyl sulfoxide, pyridine, water, dimethylformamide,
methanol, saline, chloroform, propanol, ethanol, isobutanol,
formamide, butanol, isopropanol, tetrahydrofuran, dioxane,
chloroform, dichloromethane, dichloroethane, etc.
[0810] In alternative embodiments, the chromatographic step
comprises column chromatography using an ion exchange resin such as
but not limited to RediSep.RTM. Rf SAX, Dowex.RTM. Marathon C Na,
Marathon C H, Marathon MSC-1 or Dowex Marathon WBA.TM. column, and
eluting can be with a 20:1; 15:1; 10:1, 5:1; 4.5:1; 4:1; 3.5:1; 3:1
2:1; 1.9:1; 1.8:1; 1.7:1; 1.6:1; 1.5:1; 1.4:1; 1.3:1; 1.2:1; 1.1:1;
1.01:1; 1.001:1; 0.1:1 mixture of elution solutions such as
chloroform, dichloromethane, dichloroethane methanol, ethanol,
propanol, dimethyl sulfoxide, water, dimethylformamide, methanol,
ethylene dichloride, chloroform, propanol, ethanol, isobutanol,
formamide, methylene dichloride, butanol, isopropanol,
tetrahydrofuran, dioxane, dichloromethane, dichloroethane, acetic
acid, carbonic acid, glycolic acid, benzoic, methanoic, formic
acid, oxalic acid, sodium hydroxide, potassium hydroxide, ammonium
hydroxide, ammonium carbonate, etc, and/or water. Any suitable
combination of ion exchange phase column packing material and
solvent having separation characteristics suitable for use in
purification of cannabinoids and cannabinoids acid can be used with
equivalent effect.
[0811] In alternative embodiments, the column eluate is collected
in several fractions. In alternative embodiments, the fractions are
tested for the presence of the desired cannabinoid or cannabinoid
acid using a suitable analytical technique, and those fractions
containing the highest amounts of the desired cannabinoid or
cannabinoid acid. In alternative embodiments, solvent is then
removed from the selected fractions, optionally by rotary
evaporation.
[0812] In alternative embodiments, the fractions containing a
product are enriched in a given cannabinoid acid obtained from the
elution chromatographic step or steps, and the fractions containing
a product enriched in a given cannabinoid acid obtained from
elution reverse phase step or steps are re-dissolved in an elution
solution. In alternative embodiments, the fractions containing a
product enriched in a given cannabinoid acid such as
tetrahydrocannabinolic acid (THCA), are dissolved in an elution
solution and subjected to an ion exchange chromatographic
purification step to produce substantially pure extracts. In
alternative embodiments, the product of the ion exchange
chromatographic step is collected in multiple fractions, which can
then be tested for the presence of the desired cannabinoid or
cannabinoid acid using any suitable analytical technique. Fractions
enriched in the desired cannabinoid or cannabinoid acid can then be
selected for further purification. In alternative embodiments, the
elution solution is removed from the selected fractions or
optionally additional solution is added to change the polarity
and/or hydrogen potential of the solution contained in the selected
fraction. An exemplary embodiment comprises: the fractions enriched
in a given cannabinoid obtained from the elution ion exchange
chromatographic step are mixed with a solvent of the opposite
polarity thereby causing a concentration of the cannabinoids in the
second solvent phase. The solvent can then be removed.
[0813] In alternative embodiments, the selected fraction is then
subjected to one or more additional ion exchange chromatographic
steps and one or more isocratic or gradient elution solutions
modifications. The ion exchange chromatographic step can comprise
column chromatography in fixed or continuous mode, and can be based
on molecular sizing and polarity ion exchange resin such as but not
limited to RediSep.RTM. Rf SAX, Dowex.RTM. Marathon C Na, Marathon
C H, Marathon MSC-1 or Dowex Marathon WBA.TM.
[0814] In alternative embodiments, various different elution
solutions can be used in combination with this type of matrix, for
example dimethyl sulfoxide, water, dimethylformamide, methanol,
ethylene dichloride, chloroform, propanol, ethanol, isobutanol,
formamide, methylene dichloride, butanol, isopropanol,
tetrahydrofuran, dioxane, acetic acid, carbonic acid, glycolic
acid, benzoic acid, formic acid, oxalic acid, sodium hydroxide,
potassium hydroxide, ammonium hydroxide, ammonium carbonate,
etc.
[0815] In alternative embodiments, essential features of the
process are the same for purification of all cannabinoids and
cannabinoid acids. Cannabis plants generally contain complex
mixtures of cannabinoid acids and cannabinoids, although depending
on the variety of cannabis one type of cannabinoid may
pre-dominate. The purpose of the elution (e.g., gradient elution)
chromatographic steps (ii) is to separate the various cannabinoid
and/or cannabinoid/cannabinoid acid components of the crude plant
extract loaded in step (i), as described above, into substantially
pure fractions or substantially pure mixtures of fractions which
are then optionally subjected to isomerization reactions and/or
elimination reactions and/or additional gradient elution
chromatography comprising normal phase, reverse phase and/or ion
exchange.
[0816] In alternative embodiments, the product of the
chromatographic step is collected in multiple fractions, which can
then be tested for the presence of the desired
cannabinoid/cannabinoid acid using any suitable analytical
technique. Fractions enriched in the desired
cannabinoid/cannabinoid acid can then be selected for further
purification Hence, the same simple process steps can be adapted
for purification of essentially any plant-derived cannabinoid or
cannabinoid acid. Selectivity for different cannabinoids or
cannabinoid acids may be enhanced by selection of appropriate
starting plant material. By way of example, if it is desired to
prepare substantially pure .DELTA..sup.9 THC or .DELTA..sup.9 THCA
then "high THC" cannabis plants can be selected as the starting
material. Alternatively, if it is desired to prepare substantially
pure CBD or CBDA then "high CBD" cannabis plants can be selected as
the starting material. It is to be understood that processes as
provided herein are of general utility and are not limited to the
use of particular Cannabis varieties as the starting material. The
precise cannabinoid content of any particular Cannabis plant
material can be qualitatively and quantitatively determined using
analytical techniques well known to those skilled in the art, such
as thin-layer chromatography or high-performance liquid
chromatography (HPLC). Thus, one may screen a range of various
Cannabis plants and select those having a high content of the
desired cannabinoid acid or cannabinoid for use as starting
material in a process as provided herein.
[0817] With the use of conventional selective breeding techniques
it is possible to develop cannabis varieties (chemovars) having
varying cannabinoid content. Select cannabis varieties (chemovars)
have relatively high content of CBD, or of the minor cannabinoids
.DELTA..sup.9-tetrahydrocannabivarin (.DELTA..sup.9-THCV),
cannabigerol (CBG) or cannabichromene (CBC). General protocols for
growing of medicinal cannabis and for testing the cannabinoid
content of cannabis plants are described in International patent
application WO 02/064109.
[0818] In alternative embodiments, methods further provide for the
generation of a substantially pure preparation of .DELTA..sup.9 THC
having a chromatographic purity of greater than about 75%, 80%,
90%, 95%, 96%, 97%, 98%, or 99% or more by area normalization of an
HPLC profile. The preparation can be a semi-solid at room
temperature. The preparation can comprises less than about 1.5%,
less than about 0.4%, or less than about 0.2%; or, less than about
0.1% CBD (w/w), less than about 0.5%, less than about 0.4%, or less
than about 0.2%, or less than about 0.1% CBD (w/w) as analyzed by
HPLC.
[0819] In alternative embodiments, the pure .DELTA..sup.8-THC and
.DELTA..sup.9-THC provided by exemplary methods as provided herein
have utility as an active pharmaceutical agent, and is also useful
as a chromatographic standard, particularly as a comparative
standard in the qualitative analysis of botanical drug substances
derived from cannabis. The availability of highly pure
.DELTA..sup.8-THC and .DELTA..sup.9-THC will also facilitate
studies of the pharmacology of .DELTA..sup.8-THC and
.DELTA..sup.9-THC mixtures.
[0820] In alternative embodiments, an exemplary method for
preparation of substantially pure .DELTA..sup.8-THC and
.DELTA..sup.9 THC comprises: [0821] i) obtaining an ethanolic
solution of a botanical drug substance from cannabis or hemp plant
material, [0822] ii) passing the solution obtained in step i)
through a filter, and collecting the eluate, [0823] iii) optionally
substantially remove solvent from the eluate by rotary evaporation
to give a cannabinoid enriched fraction, [0824] iv) optionally
adding solvent to the eluate, [0825] v) passing a solution of the
resulting cannabinoid enriched extract through a column packed with
a stationary phase resin such as DOWEX.RTM. MARATHON C Na.TM.,
MARATHON C H.TM., and conducting a gradient elution of
50:1;20:1;10:1; 9:1; 8:1; 7:1; 6:1; 5:1; 4:1; 3:1; 2:1; 1; 1; 0.5:1
;0.005:1; 0.0005:1 chloroform/methanol or ethanol, [0826] vi)
adjusting hydrogen potential of said solution, [0827] vii)
collecting .DELTA..sup.9-THC and CBD enriched fractions and
optionally removing solvent by rotary evaporation, [0828] viii)
mixing the collected .DELTA..sup.9 THC and CBD with a reaction
solvent and a catalyst for a period of time and adding a
neutralizing agent and removing catalyst and neutralizing agent by
filtration to generate a reduced CBD and enriched .DELTA..sup.8-THC
and .DELTA..sup.9-THC mixture, optionally removing reaction
solvent, [0829] ix) mixing reduced CBD and enriched
.DELTA..sup.8-THC and .DELTA..sup.9-THC mixture with optionally
additional reaction solvent and a stabilizing agent, mixing
stabilizing agent for a period of time [0830] x) optionally
removing the reaction solvent, [0831] x) optionally re-dissolving
the enriched .DELTA..sup.8 THC, .DELTA..sup.9 THC and reduced CBD
prepared in steps viii) through x) and passing the solution through
a column packed with reverse phase resin, and conducting a gradient
elution of 10:1; 9:1; 8:1; 7:1; 6:1; 5:1; 4:1; 3:1; 2:1; 1;1
methanol/water, [0832] xiii) optionally re-dissolving the crude
.DELTA..sup.8 THC, .DELTA..sup.9 THC and reduced CBD prepared in
steps viii) through x) and passing the solution through a column
packed with an ion exchange resin such as DOWEX.RTM. MARATHON C
Na.TM., MARATHON C H.TM., and conducting an elution of 50:1; 20:1;
10:1; 9:1; 8:1; 7:1; 6:1; 5:1; 4:1; 3:1; 2:1; 1;1; 0.5:1; 0.005: 1;
0.0005:1 chloroform and polar solvent mixture. [0833] xiv)
adjusting hydrogen potential of said solution [0834] xv) collecting
the THC enriched fractions and removing solvent by rotary
evaporation to give a semi-solid preparation of THC.
Example 12: Conversion of CBD to .DELTA..sup.8-THC and
.DELTA..sup.9-THC
[0835] This Example describes an exemplary method for the
conversion of CBD to .DELTA..sup.8-THC and .DELTA..sup.9-THC.
[0836] CBD (1 g) was added to 6.4 ml of reaction solvent and 1%
p-toluenesulfonic acid. In this example, the mixture was reacted
for 48 hours, although other time periods can also be used, as
discussed below. It was then diluted with ether (20 ml) and poured
into water, The upper layer was separated, washed with aqueous 5%
NaHCO.sub.3, then with water, dried over MgSO.sub.4 and evaporated.
GC-MS analysis on the crude product, showed the presence of 1.1%
CBD; 18.4% .DELTA..sup.8-THC and 80.0% .DELTA..sup.9-THC. The crude
product was then subjected to column chromatography. In the example
described above, normal phase HPLC separation is used wherein the
column is for example a silica gel and the mobile phase is an
organic solvent mixture introduced as a gradient described in the
invention. In other embodiments, reverse phase HPLC separation is
used.
[0837] The p-toluenesulfonic acid is used as a catalyst in the
above example. It is of note that boron trifluoride could also be
used as a catalyst, as could a number of other Lewis acids or
non-oxidizing acid catalysts. The exact proportion is not essential
to the reaction proceeding. Other solvents can also be used, for
example, benzene, toluene, chloroform, dichloromethane, etc.
[0838] In other embodiments, anhydrous MgSO.sub.4 or another
suitable agents such as Na.sub.2SO.sub.4, CaSO.sub.4, and
CaCl.sub.2, known in the art is used in place of the
MgSO.sub.4.
Example 13: Conversion of CBD to .DELTA..sup.8-THC and
.DELTA..sup.9-THC
[0839] This Example describes an exemplary method for the
conversion of CBD to .DELTA..sup.8-THC and .DELTA..sup.9-THC.
[0840] A hemp extract was obtained by extraction of hemp in
ethanol. The hemp extract was composed of CBD, THC, cannabinoids
and other components and subjected to conversion of CBD to THC
without further purification. The hemp extract and 1.about.5 mol %
p-toluenesulfonic acid were sequentially added to 6.4 mL reaction
solvent. In this example, the reaction mixture was stirred at room
temperature for 24 hours, although other time periods can also be
used, as discussed below. At some time intervals, the reaction
mixture was diluted with ether (20 mL) and poured into water. The
upper layer was separated, washed with aqueous 5% NaHCO.sub.3,
dried over MgSO.sub.4 and concentrated on a rotary evaporator.
GC-MS analysis on the crude product showed the conversion of CBD to
.DELTA..sup.8-THC and .DELTA..sup.8-THC, for example, 0% CBD; 98%
.DELTA..sup.9-THC; 2% .DELTA..sup.8-THC after 24 hours in the
presence of 3 mol % p-toluenesulfonic acid. The crude product was
then subjected to column chromatography. In the example described
above, normal phase HPLC separation is used wherein the column is
for example a silica gel and the mobile phase is an organic solvent
mixture introduced as a gradient described in the invention. In
other embodiments, reverse phase HPLC separation is used.
[0841] The p-toluenesulfonic acid is used as a catalyst in the
above example. Boron trifluoride could also be used as a catalyst,
as could a number of other Lewis acids or non-oxidizing acids known
in the art. Other solvents can also be used, for example, benzene,
toluene, chloroform, dichloromethane, etc.
[0842] In other embodiments, anhydrous MgSO.sub.4 or another
suitable agents such as Na.sub.2SO.sub.4, CaSO.sub.4, and
CaCl.sub.2, known in the art is used in place of the
MgSO.sub.4.
[0843] A number of embodiments of the invention have been
described. Nevertheless, it can be understood that various
modifications may be made without departing from the spirit and
scope of the invention. Accordingly, other embodiments are within
the scope of the following claims.
* * * * *