U.S. patent application number 17/465253 was filed with the patent office on 2021-12-23 for compositions for treatment and methods for making and using the same.
The applicant listed for this patent is ASP Global Manufacturing GmbH. Invention is credited to Shokouh Farvid, Keyvan Nowruzi, Navid Omidbakhsh.
Application Number | 20210395653 17/465253 |
Document ID | / |
Family ID | 1000005822487 |
Filed Date | 2021-12-23 |
United States Patent
Application |
20210395653 |
Kind Code |
A1 |
Farvid; Shokouh ; et
al. |
December 23, 2021 |
COMPOSITIONS FOR TREATMENT AND METHODS FOR MAKING AND USING THE
SAME
Abstract
A detergent composition, a method of making the detergent
composition, and a method of use thereof are provided. The
detergent composition comprises at least 0.001% by weight of an
antimicrobial agent, based on the total weight of the composition;
an enzyme; and at least 0.01% by weight of a hydrotrope, based on
the total weight of the composition.
Inventors: |
Farvid; Shokouh; (Irvine,
CA) ; Omidbakhsh; Navid; (Irvine, CA) ;
Nowruzi; Keyvan; (Irvine, CA) |
|
Applicant: |
Name |
City |
State |
Country |
Type |
ASP Global Manufacturing GmbH |
Schaffhausen |
|
CH |
|
|
Family ID: |
1000005822487 |
Appl. No.: |
17/465253 |
Filed: |
September 2, 2021 |
Related U.S. Patent Documents
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Application
Number |
Filing Date |
Patent Number |
|
|
16452899 |
Jun 26, 2019 |
11130930 |
|
|
17465253 |
|
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62691224 |
Jun 28, 2018 |
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Current U.S.
Class: |
1/1 |
Current CPC
Class: |
C11D 3/43 20130101; A61B
90/70 20160201; A61B 2090/701 20160201; C11D 3/12 20130101; C11D
3/3418 20130101; B08B 3/08 20130101; C11D 3/38618 20130101; A01N
63/50 20200101; C11D 3/48 20130101; C11D 3/30 20130101 |
International
Class: |
C11D 3/48 20060101
C11D003/48; A61B 90/70 20060101 A61B090/70; B08B 3/08 20060101
B08B003/08; C11D 3/12 20060101 C11D003/12; C11D 3/30 20060101
C11D003/30; C11D 3/34 20060101 C11D003/34; C11D 3/386 20060101
C11D003/386; C11D 3/43 20060101 C11D003/43; A01N 63/50 20060101
A01N063/50 |
Claims
1-25. (canceled)
26. A detergent composition, comprising: at least 1% by weight of
an antimicrobial agent, based on the total weight of the detergent
composition; an enzyme; at least 1% by weight of a hydrotrope,
based on the total weight of the detergent composition; and water,
wherein the composition comprises 10% or less by weight of a
non-ionic surfactant, based on the total weight of the detergent
composition.
27. The detergent composition of claim 26, wherein the hydrotrope
comprises at least one of an alkanoic acid, an aromatic sulfonic
acid, an aromatic carboxylic acid, and a salt of any thereof.
28. The detergent composition of claim 26, wherein the
antimicrobial agent comprises at least one of a biguanide compound
and a quaternary ammonium compound.
29. The detergent composition of claim 28, wherein the biguanide
compound comprises at least one of chlorohexidine and a salt
thereof.
30. The detergent composition of claim 26, wherein the enzyme
comprises at least one of a lipase, a protease, a peptidase, an
amylase, a glycosidase, a cellulase, DNAse, and a nuclease.
31. The detergent composition of claim 26, wherein the composition
has a pH in a range of 6 to 11.
32. The detergent composition of claim 26, further comprising at
least one of: at least 0.0001% by weight of a buffer, based on the
total weight of the detergent composition; at least 0.0001% by
weight of a pH adjusting agent, based on the total weight of the
detergent composition; at least 0.01% by weight of a solvent, based
on the total weight of the detergent composition; at least one of a
chelating agent and a salt; at least 0.005% by weight of the
non-ionic surfactant, based on the total weight of the composition;
and either no boron-containing compound or only an incidental
amount.
33. The detergent composition of claim 26, further comprising at
least 10% by weight of the water, based on the total weight of the
detergent composition.
34. The detergent composition of claim 26, further comprising at
least 5% by weight of the hydrotrope, based on the total weight of
the detergent composition.
35. The detergent composition of claim 26, further comprising at
least 2% by weight of the anti-microbial agent, based on the total
weight of the detergent composition.
36. The detergent composition of claim 26, wherein the enzyme is
present in the composition in an effective amount to remove and/or
eliminate debris and/or bioburden.
37. A diluted composition formed by adding a diluent to the
detergent composition of claim 26.
38. The diluted composition of claim 37, wherein the diluent
comprises water.
39. A method comprising forming a diluted composition by adding a
diluent to the detergent composition of claim 26.
40. The method of claim 39, wherein the diluent comprises
water.
41. A detergent composition comprising: 0.1% to 20% by weight of a
biguanide compound, based on the total weight of the detergent
composition; an enzyme present in the detergent composition in an
effective amount to remove and/or eliminate debris and/or
bioburden; 1% to 30% by weight of a hydrotrope, based on the total
weight of the detergent composition; at least 20% by weight of
water, based on the total weight of the detergent composition; and
2% to 10% by weight of a non-ionic surfactant, based on the total
weight of the detergent composition.
42. The composition of claim 41, wherein the hydrotrope comprises
at least one of an alkanoic acid, an aromatic sulfonic acid, an
aromatic carboxylic acid, and a salt of any thereof, wherein the
biguanide compound comprises at least one of chlorohexidine and a
salt thereof, wherein the enzyme comprises at least one of a
lipase, a protease, a peptidase, an amylase, a glycosidase, a
cellulase, DNAse and a nuclease; and wherein the detergent
composition has a pH in a range of 6 to 11.
43. The detergent composition of claim 41, further comprising at
least one of: at least 0.0001% by weight of a buffer, based on the
total weight of the detergent composition; at least 0.0001% by
weight of a pH adjusting agent, based on the total weight of the
detergent composition; at least 0.01% by weight of a solvent, based
on the total weight of the detergent composition; and at least one
of a chelating agent and a salt.
44. A diluted composition formed by adding a diluent to the
detergent composition of claim 41.
45. The diluted composition of claim 44, wherein the diluent
comprises water.
46. A method comprising forming a diluted composition by adding a
diluent to the detergent composition of claim 41.
47. The method of claim 46, wherein the diluent comprises water.
Description
CROSS REFERENCE TO RELATED APPLICATIONS
[0001] This application claims the benefit of U.S. Provisional
Application No. 62/691,224 filed Jun. 28, 2018, which is
incorporated herein by reference.
FIELD
[0002] The present disclosure relates to compositions for treatment
and methods of making and using those compositions.
BACKGROUND
[0003] Various medical devices are employed for procedures in the
medical field. One such device is an endoscope that examines the
interior of a hollow organ or cavity of the body. Ensuring reusable
medical devices are treated property can inhibit or prevent
cross-contamination and the spread of disease. In this regard,
treatment solutions such as, for example, cleaning solutions and/or
antimicrobial solutions are used on medical devices and facility
surfaces.
SUMMARY
[0004] In one aspect, the present disclosure provides a detergent
composition. The detergent composition comprises at least 0.001% by
weight of an antimicrobial agent, based on the total weight of the
composition, an enzyme, and at least 0.01% by weight of a
hydrotrope, based on the total weight of the composition.
[0005] In another aspect, the present disclosure provides a method
of making a detergent composition. The method comprises combining,
based on the total weight of the composition, at least 0.001% by
weight of an antimicrobial agent, at least 0.01% by weight of a
hydrotrope, and an enzyme.
[0006] In yet another aspect, the present disclosure provides a
method for cleaning an object. The method comprises applying a
detergent composition to the object, thereby cleaning the object.
The detergent composition comprises, based on the total weight of
the composition, at least 0.001% by weight of an antimicrobial
agent, at least 0.01% by weight of a hydrotrope, and an enzyme.
[0007] It is understood that the inventions described in this
specification are not limited to the examples provided in this
Summary. Various other aspects are described and exemplified
herein.
BRIEF DESCRIPTION OF THE DRAWINGS
[0008] The present disclosure contains at least one drawing
executed in color. Copies of the present disclosure with color
drawing(s) will be provided by the Office upon request and payment
of the necessary fee.
[0009] The features and advantages of the examples, and the manner
of attaining them, will become more apparent and the examples will
be better understood by reference to the following description of
examples taken in conjunction with the accompanying drawings,
wherein:
[0010] FIG. 1 is a depiction of a system for treatment of an object
utilizing a detergent composition according to the present
disclosure.
[0011] FIG. 2A-D shows photographs of plastic or stainless steel
coupons treated with a diluted detergent composition according to
the present disclosure.
[0012] FIG. 2A shows a photograph of a stainless steel coupon
treated with a diluted detergent composition according to the
present disclosure that was supplemented with chlorhexidine
gluconate (CHG).
[0013] FIG. 2B shows a photograph of a plastic coupon treated with
a diluted detergent composition according to the present disclosure
that was supplemented with CHG.
[0014] FIG. 2C shows a photograph of a stainless steel coupon
treated with a diluted detergent composition according to the
present disclosure that was not supplemented with CHG.
[0015] FIG. 2D shows a photograph of a plastic coupon treated with
a diluted detergent composition according to the present disclosure
that was not supplemented with CHG.
[0016] FIG. 3A-D shows photographs of plastic or stainless steel
coupons treated with a diluted detergent composition, C1.
[0017] FIG. 3A shows a photograph of a stainless steel coupon
treated with a diluted detergent composition, C1, which was
supplemented with CHG.
[0018] FIG. 3B shows a photograph of a plastic coupon treated with
a diluted detergent composition, C1, which was supplemented with
CHG.
[0019] FIG. 3C shows a photograph of a stainless steel coupon
treated with a diluted detergent composition, C1, which was not
supplemented with CHG.
[0020] FIG. 3D shows a photograph of a plastic coupon treated with
a diluted detergent composition, C1, which was not supplemented
with CHG.
[0021] FIG. 4A-D shows photographs, when available, of plastic or
stainless steel coupons treated with a diluted detergent
composition, C2.
[0022] FIG. 4A indicates that CHG was insoluble in a diluted
detergent composition, C2.
[0023] FIG. 4B indicates that CHG was insoluble in a diluted
detergent composition, C2.
[0024] FIG. 4C shows a photograph of a stainless steel coupon
treated with a diluted detergent composition, C2, which was not
supplemented with CHG.
[0025] FIG. 4D shows a photograph of a plastic coupon treated with
a diluted detergent composition, C2, which was not supplemented
with CHG.
[0026] FIG. 5A-D shows photographs, when available, of plastic or
stainless steel coupons treated with a diluted detergent
composition, C3.
[0027] FIG. 5A indicates that CHG was insoluble in a diluted
detergent composition, C3.
[0028] FIG. 5B indicates that CHG was insoluble in a diluted
detergent composition, C3.
[0029] FIG. 5C shows a photograph of a stainless steel coupon
treated with a diluted detergent composition, C3, which was not
supplemented with CHG.
[0030] FIG. 5D shows a photograph of a plastic coupon treated with
a diluted detergent composition, C3, which was not supplemented
with CHG.
[0031] FIG. 6A-D shows photographs of plastic or stainless steel
coupons treated with a diluted detergent composition, C4.
[0032] FIG. 6A shows a photograph of a stainless steel coupon
treated with a diluted detergent composition, C4, which was
supplemented with CHG.
[0033] FIG. 6B shows a photograph of a plastic coupon treated with
a diluted detergent composition, C4, which was supplemented with
CHG.
[0034] FIG. 6C shows a photograph of a stainless steel coupon
treated with a diluted detergent composition, C4, which was not
supplemented with CHG.
[0035] FIG. 6D shows a photograph of a plastic coupon treated with
a diluted detergent composition, C4, which was not supplemented
with CHG.
[0036] FIG. 7A-D shows photographs of plastic or stainless steel
coupons treated with a diluted detergent composition, C5.
[0037] FIG. 7A shows a photograph of a stainless steel coupon
treated with a diluted detergent composition, C5, which was
supplemented with CHG.
[0038] FIG. 7B shows a photograph of a plastic coupon treated with
a diluted detergent composition, C5, which was supplemented with
CHG.
[0039] FIG. 7C shows a photograph of a stainless steel coupon
treated with a diluted detergent composition, C5, which was not
supplemented with CHG.
[0040] FIG. 7D shows a photograph of a plastic coupon treated with
a diluted detergent composition, C5, which was not supplemented
with CHG.
[0041] FIG. 8A-D shows photographs, when available, of plastic or
stainless steel coupons treated with a diluted detergent
composition, C6.
[0042] FIG. 8A indicates that CHG was insoluble in a diluted
detergent composition, C6.
[0043] FIG. 8B indicates that CHG was insoluble in a diluted
detergent composition, C6.
[0044] FIG. 8C shows a photograph of a stainless steel coupon
treated with a diluted detergent composition, C6, which was not
supplemented with CHG.
[0045] FIG. 8D shows a photograph of a plastic coupon treated with
a diluted detergent composition, C6, which was not supplemented
with CHG.
[0046] Corresponding reference characters indicate corresponding
parts throughout the several views. The exemplifications set out
herein illustrate certain examples, in one form, and such
exemplifications are not to be construed as limiting the scope of
the examples in any manner.
DETAILED DESCRIPTION
[0047] Certain exemplary aspects of the present disclosure will now
be described to provide an overall understanding of the principles
of the composition, function, manufacture, and use of the
compositions and methods disclosed herein. One or more examples of
these aspects are illustrated in the accompanying drawing. Those of
ordinary skill in the art will understand that the compositions and
methods specifically described herein and illustrated in the
accompanying drawings are non-limiting exemplary aspects and that
the scope of the various examples of the present invention is
defined solely by the claims. The features illustrated or described
in connection with one exemplary aspect may be combined with the
features of other aspects. Such modifications and variations are
intended to be included within the scope of the present
invention.
[0048] Reference throughout the specification to "various
examples," "some examples," "one example," or "an example", or the
like, means that a particular feature, structure, or characteristic
described in connection with the example is included in at least
one example. Thus, appearances of the phrases "in various
examples," "in some examples," "in one example", or "in an
example", or the like, in places throughout the specification are
not necessarily all referring to the same example. Furthermore, the
particular features, structures, or characteristics may be combined
in any suitable manner in one or more examples. Thus, the
particular features, structures, or characteristics illustrated or
described in connection with one example may be combined, in whole
or in part, with the features structures, or characteristics of one
or more other examples without limitation. Such modifications and
variations are intended to be included within the scope of the
present examples.
[0049] In this specification, unless otherwise indicated, all
numerical parameters are to be understood as being prefaced and
modified in all instances by the term "about", in which the
numerical parameters possess the inherent variability
characteristic of the underlying measurement techniques used to
determine the numerical value of the parameter. At the very least,
and not as an attempt to limit the application of the doctrine of
equivalents to the scope of the claims, each numerical parameter
described herein should at least be construed in light of the
number of reported significant digits and by applying ordinary
rounding techniques.
[0050] Also, any numerical range recited herein includes all
sub-ranges subsumed within the recited range. For example, a range
of "1 to 10" includes all sub-ranges between (and including) the
recited minimum value of 1 and the recited maximum value of 10,
that is, having a minimum value equal to or greater than 1 and a
maximum value equal to or less than 10. Any maximum numerical
limitation recited in this specification is intended to include all
lower numerical limitations subsumed therein and any minimum
numerical limitation recited in this specification is intended to
include all higher numerical limitations subsumed therein.
Accordingly, Applicant reserves the right to amend this
specification, including the claims, to expressly recite any
sub-range subsumed within the ranges expressly recited. All such
ranges are inherently described in this specification such that
amending to expressly recite any such sub-ranges would comply with
the requirements of 35 U.S.C. .sctn. 112 and 35 U.S.C. .sctn.
132(a).
[0051] The present disclosure relates to compositions for treatment
and methods of making and using those compositions. Objects of the
present disclosure can undergo a treatment process as set forth
herein to prevent cross-contamination and the spread of disease. As
used herein, a "treatment process" may be a cleaning process, a
disinfecting process, the like, and combinations thereof. A
treatment process may be either manual, automated, or some
combination thereof, and may utilize a treatment agent. As used
herein, a "treatment agent" can comprise at least one of a cleaning
agent and an antimicrobial agent. As used herein a "cleaning
process" means a treatment process employing a cleaning agent that
removes and/or eliminates debris such as, for example, a body fluid
(e.g., blood, urine, saliva) a dirt, a dust, a particle, an oil, a
protein, a carbohydrate, and the like. As used herein, a "cleaning
agent" means a type of treatment agent that removes and/or
eliminates debris during a cleaning process such as, for example, a
surfactant and/or a detergent.
[0052] A disinfecting process can remove and/or eliminate a
bioburden from an object. A bioburden may be, for example, a
bacterium (e.g., mycobacterium, bacterial spores), an archaeon, a
eukaryote, a virus, a fungus, and/or other forms of biological
agents. Bacterial spores (e.g., endospores) are a form of bacteria
which are dormant and highly resistive to physical and chemical
degradation. As used herein, a "disinfecting process" means a
treatment process that substantially removes a bioburden. As used
herein, "substantially remove" means that at least 99% of the
bioburden has been removed from the object such as, for example, at
least 99.9% of the bioburden, at least 99.99% of the bioburden, at
least 99.999% of the bioburden, or at least 99.9999% of the
bioburden has been removed from the object. The disinfection
process may include, for example, the addition of heat, an
antimicrobial agent, irradiation, pressure, and combinations
thereof. The antimicrobial agent may comprise a chemical capable of
disinfection.
[0053] As used herein, a "component" of the detergent composition
of the present disclosure is meant to mean any chemical substance
that can be added and/or can be a part of the detergent
composition. For example, a component of the detergent composition
can be water, an alcohol, an antimicrobial agent, a hydrotrope, a
surfactant, a buffer, a solvent, an enzyme, a chelating agent, a
salt, the like, and combinations thereof.
[0054] Detergent compositions comprising an enzyme can aid in
cleaning an object. The enzyme can remove and/or eliminate debris.
However, the enzyme, by itself, may not provide a sufficient
reduction of bioburden to disinfect an object. Thus, an additional
disinfecting process employing an antimicrobial agent can be
performed simultaneously with or subsequent to the first cleaning
step to prepare the object for subsequent use.
[0055] However, it was previously believed by those of ordinary
skill in the art that an antimicrobial agent reduces the enzymatic
activity of the enzyme of the detergent composition, possibly via
denaturation of the enzyme's three-dimensional structure, thereby
adversely affecting and diminishing the ability of the enzyme to
remove and/or eliminate debris and/or bioburden. Additionally, the
antimicrobial agent was thought to similarly denature elements of
debris and/or bioburden making them less likely to be removed
during a treatment process. Thus, previously, it was believed that
an antimicrobial agent was incompatible with a detergent comprising
an enzyme.
[0056] Surprisingly, it has been found that the addition of an
antimicrobial agent to a detergent composition in combinations
and/or amounts provided herein can enable a detergent composition
already used for cleaning to also be used for disinfection
processes. Thus, it has been found that the cleaning and
disinfecting processes can be combined, and the efficiency of
treating objects can be increased. The cleaning and disinfecting
can be combined.
[0057] Accordingly, provided herein are detergent compositions
comprising an antimicrobial agent and an enzyme, methods of making
the detergent compositions, and methods of using the detergent
compositions. The detergent compositions of the present disclosure
can comprise a cleaning agent and an antimicrobial agent. The
detergent compositions of the present disclosure can comprise an
enzyme that can effectively remove and/or eliminate debris and/or
bioburden from an object in the presence of an antimicrobial agent
that can disinfect the object. The detergent compositions set forth
herein can clean and disinfect the object.
[0058] In one example, the present disclosure provides a detergent
composition comprising an antimicrobial agent, an enzyme, and a
hydrotrope.
[0059] The antimicrobial agent can comprise at least one of a
biguanide compound and a quaternary ammonium compound. Quaternary
ammonium compounds comprise a nitrogen atom covalently bonded to
four R-groups. For example, quaternary ammonium compounds can
comprise Formula 1 below.
##STR00001##
where R.sub.1-4 are each an alkyl or aryl group, and R.sub.1-4 may
each be the same or different.
[0060] As used herein a "biguanide compound" means at least one of
a bisbiguanide compound, a biguanide compound, and a polybiguanide
compound. Polybiguanide compounds can comprise Formula 2 below.
##STR00002##
where R.sub.5 is an alkyl or an aryl, R.sub.5 may be halogen
substituted; and [0061] n is in a range of 1 to 50.
[0062] For example, when n is 2, the polybiguanide compounds can
comprise Formula 3 below.
##STR00003##
where R.sub.6 and R.sub.5 are each an alkyl or an aryl, R.sub.6 and
R.sub.5 may be halogen substituted, and R.sub.6 and R.sub.5 may be
the same or different; and [0063] R.sub.7 is an alkyl comprising 3
to 10 carbon atoms.
[0064] The antimicrobial agent in the detergent compositions of the
present disclosure can remove and/or eliminate bioburden. The
antimicrobial agent can disinfect an object (e.g., via disruption
of biological membranes or denaturation of proteins). The
antimicrobial agent can be present in the detergent compositions of
the present disclosure in any effective amount. For example, the
detergent composition can comprise at least 0.001% antimicrobial
agent by weight based on the total weight of the detergent
composition such as, for example, at least 0.01% antimicrobial
agent by weight, at least 0.1% antimicrobial agent by weight, at
least 0.5% antimicrobial agent by weight, at least 1% antimicrobial
agent by weight, at least 2% antimicrobial agent by weight, at
least 3% antimicrobial agent by weight, at least 4% antimicrobial
agent by weight, at least 5% antimicrobial agent by weight, at
least 10% antimicrobial agent by weight, or at least 15%
antimicrobial agent by weight. The detergent composition can
comprise 20% or less antimicrobial agent by weight based on the
total weight of the detergent composition such as, for example, 15%
or less antimicrobial agent by weight, 10% or less antimicrobial
agent by weight, 5% or less antimicrobial agent by weight, 4% or
less antimicrobial agent by weight, 3% or less antimicrobial agent
by weight, 2% or less antimicrobial agent by weight, 1% or less
antimicrobial agent by weight, 0.5% or less antimicrobial agent by
weight, 0.1% or less antimicrobial agent by weight, or 0.01% or
less antimicrobial agent by weight. The detergent composition can
comprise 0.001% to 20% antimicrobial agent by weight based on the
total weight of the detergent composition such as, for example,
0.001% to 5% antimicrobial agent by weight, 0.01% to 5%
antimicrobial agent by weight, 0.1% to 5% antimicrobial agent by
weight, 1% to 5% antimicrobial agent by weight, 1% to 10%
antimicrobial agent by weight, 5% to 15% antimicrobial agent by
weight, or 1% to 20% antimicrobial agent by weight.
[0065] The biguanide compound, if present, can comprise at least
one of chlorhexidine (e.g.,
N,N''''1,6-Hexanediylbis[N'-(4-chlorophenyl)(imidodicarbonimidic
diamide)]), alexidine (e.g.,
1,1'-(1,6-Hexanediyl)bis{2-[N'-(2-ethylhexyl)carbamimidoyl]guanidine}),
octenidine, (e.g.,
N-octyl-1-[10-(4-octyliminopyridin-1-yl)decyl]pyridin-4-imine), a
polybiguanide such as Polyhexanide (polyhexamethylene biguanide),
and a salt of any thereof.
[0066] The quaternary ammonium compound, if present, can comprise,
for example, at least one of n-alkyl dimethyl benzyl ammonium
chloride, didecyl dimethyl ammonium chloride, and n-alkyl dimethyl
ethylbenzyl ammonium chloride, and various other suitable
quaternary ammonium compounds as known in the art. The quaternary
ammonium compound can comprise n-alkyl dimethyl benzyl ammonium
chloride and didecyl dimethyl ammonium chloride. The quaternary
ammonium compound may be BTC 1210.RTM., available from Stepan
Company, Northfield, Ill. BTC 1210.RTM. can comprise n-alkyl
dimethyl benzyl ammonium chloride and didecyl dimethyl ammonium
chloride.
[0067] The detergent composition can further comprise a hydrotrope.
A hydrotrope is a substance that can solubilize hydrophobic
substances while in aqueous solution. Hydrotropes generally do not
form micelles as readily as surfactants because the hydrophobic
moieties of hydrotropes are too small to do so. The hydrotrope can
be, for example, an anionic, cationic, or nonionic hydrotrope. The
hydrotrope can be inorganic or organic and can comprise surfactant
activity. Organic solvents can be sulfonated to create a sulfonic
acid, which can then be neutralized to create a hydrotrope salt.
The hydrotrope can comprise at least one of an alkanoic acid (e.g.,
sulfonic acid, carboxylic acid), an aromatic sulfonic acid, an
aromatic carboxylic acid, and a salt of any thereof. The salt of
the alkanoic acid can be, for example, a sodium alkanoate salt. The
hydrotrope can comprise a toluenesulfonyl functional group. The
hydrotrope can comprise at least one of urea, p-toluenesulfonic
acid (e.g., 4-methylbenzene-1-sulfonic acid), xylene sulfonic acid
(e.g., 2,5-dimethylbenzenesulfonic acid), cumene sulfonic acid
(e.g., 2(or 4)-(isopropyl)benzenesulphonic acid), and a salt of any
thereof. If an anionic hydrotrope is employed, the hydrotrope can
comprise at least one of Cola.RTM. Trope INC, Cola.RTM. Trope OD,
and Cola.RTM. Trope CA. All three Cola.RTM. Trope substances are
available from Colonial Chemical, Inc., South Pittsburgh, Tenn.,
USA. All three Cola.RTM. Trope substances comprise sodium
alkanoate.
[0068] The hydrotrope can be present in the detergent compositions
of the present disclosure in any effective amount. For example, the
detergent compositions of the present disclosure can comprise at
least 0.01% hydrotrope by weight based on the total weight of the
detergent composition such as, for example, at least 0.1%
hydrotrope by weight, at least 1% hydrotrope by weight, at least 5%
hydrotrope by weight, at least 10% hydrotrope by weight, at least
15% hydrotrope by weight, at least 20% hydrotrope by weight, or at
least 25% hydrotrope by weight. The detergent composition can
comprise 30% or less hydrotrope by weight based on the total weight
of the detergent composition such as, for example, 25% or less
hydrotrope by weight, 20% or less hydrotrope by weight, 15% or less
hydrotrope by weight, 10% or less hydrotrope by weight, 5% or less
hydrotrope by weight, 1% or less hydrotrope by weight, or 0.1% or
less hydrotrope by weight. The detergent composition can comprise
0.01% to 30% hydrotrope by weight based on the total weight of the
detergent composition such as, for example, 0.1% to 30% hydrotrope
by weight, 1% to 30% hydrotrope by weight, 5% to 20% hydrotrope by
weight, 5% to 15% hydrotrope by weight, 10% to 20% hydrotrope by
weight, or 10% to 15% hydrotrope by weight.
[0069] The detergent composition can further comprise an enzyme
component. The enzyme component can be in the form of a liquid
enzyme solution or a dry, powdered component (e.g., lyophilized).
The enzyme can remove and/or eliminate debris via enzymatic
digestion (e.g., decomposition) of the debris. The enzyme can
comprise a hydrolase enzyme. The hydrolase enzyme can break
chemical bonds in the debris and/or bioburden, by the addition of a
water molecule (e.g., hydrolysis). For example, the hydrolase
enzyme can remove and/or eliminate lipids, carbohydrates, proteins,
peptides, and nucleic acids from an object. The hydrolase enzyme
can comprise at least one of a lipase, a protease, a peptidase, an
amylase, a glycosidase, a cellulase, a DNAse, and a nuclease. The
enzyme can be selected based on a pH of a detergent composition
and/or effectiveness on removing and/or eliminating a select debris
and/or bioburden.
[0070] The enzyme can be present in the detergent compositions of
the present disclosure in any effective amount. For example, the
enzyme can comprise at least 0.001% active enzyme protein by weight
based on the total dry weight of the enzyme such as, for example,
at least 0.01% active enzyme protein, at least 0.1% active enzyme
protein, at least 1% active enzyme protein, at least 5% active
enzyme protein, at least 10% active enzyme protein, or at least 15%
active enzyme protein. The enzyme can comprise 20% or less active
enzyme protein by weight based on the total dry weight of the
enzyme such as, for example, 15% or less active enzyme protein, 10%
or less active enzyme protein, 5% or less active enzyme protein, 1%
or less active enzyme protein, 0.1% or less active enzyme protein,
or 0.01% or less active enzyme protein. The enzyme can comprise
0.001% to 20% active enzyme protein by weight based on the total
dry weight of the enzyme such as, for example, 0.01% to 20% active
enzyme protein, 0.1% to 20% active enzyme protein, 0.1% to 10%
active enzyme protein, 1% to 10% active enzyme protein, or 1% to 5%
active enzyme protein.
[0071] The pH the detergent composition can be adjusted based on
the enzyme such that the enzyme has an enzymatic activity suitable
to remove and/or eliminate debris and/or bioburden. For example,
the detergent composition can have a pH of at least 6.0 such as,
for example, at least 6.5, at least 7.0, at least 7.5, at least
8.0, at least 9, at least 10, or at least 11. The detergent
composition can have a pH of less than 12 such as, for example,
less than 11, less than 10, less than 9.0, less than 8, less than
7.5, less than 7.0, or less than 6.5. The detergent composition can
have a pH in a range of 6 to 12 such as, for example, 6 to 8, 7 to
10, 7 to 9, or 8 to 11.
[0072] The pH of the detergent composition can be adjusted by
adding a pH adjusting agent. The pH adjusting agent can be, for
example, at least one of an acid and a base. The pH adjusting agent
can comprise, for example, at least one of sodium hydroxide,
potassium hydroxide, monoethanolamine, diethanolamine, and
triethanolamine. The detergent composition can comprise any
effective amount of pH adjusting agent to achieve the desired pH.
For example, the detergent composition can comprise 2% or less of a
pH adjusting agent based on the total weight of the detergent
composition such as, for example, 1% or less of a pH adjusting
agent, 0.5% or less of a pH adjusting agent, 0.1% or less of a pH
adjusting agent, 0.01% or less of a pH adjusting agent, 0.001% or
less of a pH adjusting agent. The detergent composition can
comprise at least 0.0001% of a pH adjusting agent based on the
total weight of the detergent composition such as, for example, at
least 0.001% of a pH adjusting agent, at least 0.01% of a pH
adjusting agent, at least 0.1% of a pH adjusting agent, at least
0.5% of a pH adjusting agent, or at least 1% of a pH adjusting
agent. The detergent composition can comprise 0.0001% to 2% of a pH
adjusting agent based on the total weight of the detergent
composition such as, for example, 0.1% to 2% of a pH adjusting
agent, 0.01% to 2% of a pH adjusting agent, or 0.001% to 1% of a pH
adjusting agent.
[0073] Detergent compositions of the present disclosure can further
comprise a boron-containing compound such as, for example, boric
acid (e.g., H.sub.3BO.sub.3), borax (e.g., mineral salts of boric
acid, including commercially provided, partially dehydrated salts),
and other similar boron-containing compounds. These
boron-containing compounds can improve the pH buffering, cleaning
performance, and enzyme stability of a detergent composition.
Surprisingly, however, it has been found that examples of the
detergent compositions of the present disclosure do not require
boron-containing compounds for aspects of their performance such
as, for example, enzyme stability, pH buffering, and/or cleaning
performance. Thus, it may be advantageous for the detergent
compositions of the present disclosure to comprise a limited amount
of a boron-containing compound, only incidental (i.e., trace)
amounts of a boron-containing compound, no measurable
boron-containing compound at all, or no intentionally added
boron-containing compound. For example, detergent compositions of
the present disclosure can comprise 0.1% or less by weight of a
boron-containing compound based on the total weight of the
detergent composition such as, for example, 0.01% or less by weight
of a boron-containing compound, 0.001% or less by weight of a
boron-containing compound, 0.0001% or less by weight of a
boron-containing compound, or no measurable boron-containing
compound.
[0074] The detergent composition of the present disclosure can
further comprise a buffer component. The buffer can stabilize the
pH of the detergent composition and can maintain a chemical
environment that can be compatible with other components of the
detergent composition such as, for example, the enzyme. The buffer
can comprise, for example, a conjugate acid/base pair. The
conjugate acid/base pair can comprise a zwitterion compound. The
zwitterion compound can accept and donate hydrogen ions in response
to pH changes, thereby maintaining a consistent pH. The zwitterion
compound can comprise an amino acid, such as, for example, glycine.
The conjugate acid/conjugate base pair can comprise, for example,
at least one of tris(hydroxymethyl)aminomethane, a carbonate
buffer, and a phosphate buffer. If present, the buffer may be
present in the detergent compositions of the present disclosure in
any effective amount. For example, the detergent composition can
comprise 10% or less by weight of buffer based on the total weight
of the detergent composition such as, for example, 5% or less
buffer by weight, 3% or less buffer by weight, 1% or less buffer by
weight, 0.5% or less buffer by weight, 0.1% or less buffer by
weight, 0.01% or less buffer by weight, or 0.001% or less buffer by
weight. The detergent compositions of the present disclosure can
comprise at least 0.0001% by weight of buffer based on the total
weight of the detergent composition such as, for example, at least
0.001% by weight of buffer, at least 0.01% by weight of buffer, at
least 0.1% by weight of buffer, at least 0.5% by weight of buffer,
at least 1% by weight of buffer, at least 3% buffer by weight, or
at least 5% by weight of buffer. The detergent compositions of the
present disclosure can comprise 0.0001% to 10% by weight of buffer
based on the total weight of the detergent composition such as, for
example, 0.001% to 5% by weight of buffer, 0.01% to 5% by weight of
buffer, 0.1% to 5% by weight of buffer, 0.5% to 3% by weight of
buffer, or 0.5% to 2% by weight of buffer.
[0075] The detergent composition of the present disclosure can
further comprise a solvent. The solvent can assist in removing
and/or eliminating debris from the object. The solvent can enhance
solubility of the components of the detergent composition and/or
the solubility of the debris and/or bioburden. Enhancing the
solubility of the debris and/or bioburden can facilitate removal
and/or elimination of the debris and/or bioburden. The solvent can
comprise, for example, at least one of a glycol ether, propylene
glycol, ethylene glycol, methanol, ethanol, isopropanol, and
n-propanol. The glycol ether can comprise, for example, at least
one of 2-ethoxyethanol, 2-butoxyethanol, methyl ether, and
propylene glycol n-butyl ether. If present, the solvent may be
present in the detergent compositions of the present disclosure in
any effective amount. For example, the detergent composition can
comprise at least 0.01% solvent by weight based on the total weight
of the detergent composition such as, for example, at least 0.1%
solvent by weight, at least 1% solvent by weight, at least 5%
solvent by weight, at least 10% solvent by weight, at least 11%
solvent by weight, at least 12% solvent by weight, at least 13%
solvent by weight, at least 14% solvent by weight, at least 15%
solvent by weight, at least 20% solvent by weight, at least 30%
solvent by weight, or at least 40% solvent by weight. The detergent
composition can comprise 50% or less solvent by weight based on the
total weight of the detergent composition such as, for example, 40%
or less solvent by weight, 30% or less solvent by weight, 20% or
less solvent by weight, 15% or less solvent by weight, 14% or less
solvent by weight, 13% or less solvent by weight, 12% or less
solvent by weight, 11% or less solvent by weight, 10% or less
solvent by weight, 5% or less solvent by weight, 1% or less solvent
by weight, or 0.1% or less solvent by weight. The detergent
composition can comprise 0.01% to 50% solvent by weight based on
the total weight of the detergent composition such as, for example,
0.1% to 5% solvent by weight, 5% to 20% solvent by weight, 10% to
20% solvent by weight, or 10% to 15% solvent by weight.
[0076] The detergent compositions of the present disclosure can
further comprise a salt. The salt can increase removal and/or
elimination of debris by the enzyme. The salt can act as an enzyme
stabilizer. The salt can be organic or inorganic and can comprise,
for example, at least one of calcium chloride, potassium chloride,
sodium chloride, sodium citrate, and magnesium chloride. If
present, the salt may be present in the detergent compositions of
the present disclosure in any effective amount. For example, the
detergent composition can comprise 10% or less of salt by weight,
based on the total weight of the detergent composition such as, for
example, 5% or less salt by weight, 4% or less salt by weight, 3%
or less salt by weight, 2% or less salt by weight, 1% or less salt
by weight, 0.5% or less salt by weight, 0.1% or less salt by
weight, or 0.01% or less salt by weight. The detergent composition
can comprise at least 0.001% of salt by weight, based on the total
weight of the detergent composition such as, for example, at least
0.01% of salt by weight, at least 0.1% of salt by weight, at least
0.5% of salt by weight, at least 1% of salt by weight, at least 2%
of salt by weight, at least 3% of salt by weight, at least 4% of
salt by weight, or at least 5% of salt by weight. The detergent
composition can comprise 0.001% to 10% of salt by weight, based on
the total weight of the detergent composition such as, for example,
0.1% to 5% of salt by weight or 0.001% to 0.1%.
[0077] The detergent composition of the present disclosure can
further comprise a chelating agent. The chelating agent can
increase cleaning by the detergent composition. For example, the
chelating agent can chelate a metal ion and/or chelate at the pH of
the detergent composition. The chelating agent can be a
non-phosphate chelator and/or can be biodegradable. The chelating
agent can comprise, for example, at least one of,
methylglycindiacetic acid, N,N-bis(carboxymethyl)-L-glutamic acid,
citric acid, a gluconic acid, N-(1,2-dicarboxyethyl)aspartic acid,
ethylenediamine-N, N'-disuccinic acid, and a salt of any thereof.
The chelating agent can comprise Trilon M.RTM., available from
BASF, SE, Ludwigshafen, Germany. Trilon M.RTM. can comprise
trisodium salt of methylglycindiacetic acid. The chelating agent
can comprise Dissolvine.RTM. GL-47-S, available from Akzo Nobel
N.V., Amsterdam, Netherlands. Dissolvine.RTM. GL-47-S can comprise
Tetrasodium N,N-bis(carboxymethyl)-L-glutamate. The chelating agent
can comprise Baypure.RTM. CX100, available from Lanxess AG,
Cologne, Germany. Baypure.RTM. CX100 can comprise
N-(1,2-dicarboxyethyl)aspartic acid as a sodium salt (e.g.,
Tetrasodium iminodisuccinate).
[0078] If present, the chelating agent may be present in the
detergent compositions of the present disclosure in any effective
amount. For example, the detergent composition can comprise 5% or
less of chelating agent by weight based on the total weight of the
detergent composition such as, for example, 4% or less of chelating
agent by weight, 3% or less of chelating agent by weight, 2% or
less of chelating agent by weight, 1% or less of chelating agent by
weight, 0.1% or less of chelating agent by weight, or 0.01% or less
of chelating agent by weight. The detergent composition can
comprise at least 0.005% of chelating agent by weight based on the
total weight of the detergent composition such as, for example, at
least 0.01% of chelating agent by weight, at least 0.1% of
chelating agent by weight, at least 1% of chelating agent by
weight, at least 2% of chelating agent by weight, at least 3% of
chelating agent by weight, or at least 4% of chelating agent by
weight. The detergent composition can comprise 0.005% to 5% of
chelating agent by weight based on the total weight of the
detergent composition such as, for example, 0.005% to 0.1% of
chelating agent by weight, 0.5% to 3% of chelating agent by weight,
1% to 3% of chelating agent by weight.
[0079] The detergent composition of the present disclosure can
comprise surfactants in addition to the hydrotrope, such as, for
example, a non-ionic surfactant. The non-ionic surfactant can
increase the solubility of debris and/or bioburden, and aid in the
removal of the debris and/or bioburden from the object. The
non-ionic surfactant can be low foaming. Non-ionic surfactants can
comprise, for example, at least one of a fatty alcohol ethylene
oxide/propylene oxide copolymer derivative, a
polyoxyethylene-polyoxypropylene block copolymer, and various other
non-ionic surfactants as known in the art. The non-ionic surfactant
may be Dehypon.RTM. LS 54 available from BASF SE, Ludwigshafen,
Germany. Dehypon.RTM. LS 54 can comprise a C12-15 fatty alcohol
ethylene oxide/propylene oxide copolymer derivative. The non-ionic
surfactant may be Dehypon.RTM. LS 36 available from BASF SE,
Ludwigshafen, Germany. Dehypon.RTM. LS 36 can comprise a C12-14
fatty alcohol ethylene oxide/propylene oxide copolymer derivative.
The non-ionic surfactant may be Pluronic.RTM. L62 available from
BASF SE, Ludwigshafen, Germany. Pluronic.RTM. L62 can comprise a
polyoxyethylene-polyoxypropylene block copolymer.
[0080] If present, the non-ionic surfactant may be present in the
detergent compositions of the present disclosure in any effective
amount. For example, the detergent composition can comprise at
least 0.005% of non-ionic surfactant by weight based on the total
weight of the detergent composition such as, for example, at least
0.01% of non-ionic surfactant by weight, at least 0.1% of non-ionic
surfactant by weight, at least 1% of non-ionic surfactant by
weight, at least 2% of non-ionic surfactant by weight, at least 3%
of non-ionic surfactant by weight, at least 4% of non-ionic
surfactant by weight, at least 5% of non-ionic surfactant by
weight, at least 6% of non-ionic surfactant by weight, or at least
7% of non-ionic surfactant by weight. The detergent composition can
comprise 10% or less of non-ionic surfactant by weight based on the
total weight of the detergent composition such as, for example, 7%
or less of non-ionic surfactant by weight, 6% or less of non-ionic
surfactant by weight, 5% or less of non-ionic surfactant by weight,
4% or less of non-ionic surfactant by weight, 3% or less of
non-ionic surfactant by weight, 2% or less of non-ionic surfactant
by weight, 1% or less of non-ionic surfactant by weight, 0.1% or
less of non-ionic surfactant by weight, or 0.01% or less of
non-ionic surfactant by weight. The detergent composition can
comprise 0.005% to 10% of non-ionic surfactant by weight based on
the total weight of the detergent composition such as, for example,
0.01% to 1% of non-ionic surfactant by weight, 0.5% to 7% of
non-ionic surfactant by weight, 0.5% to 6% of non-ionic surfactant
by weight, 1% to 6% of non-ionic surfactant by weight, or 2% to 6%
of non-ionic surfactant by weight.
[0081] The detergent compositions of the present disclosure can
further comprise at least 10% by weight of water based on the total
weight of the detergent composition such as, for example, at least
25% water by weight, at least 35% water by weight, at least 40%
water by weight, at least 45% water by weight, at least 50% water
by weight, at least 55% water by weight, or at least 60% water by
weight. The water content of the detergent composition can be in a
range of 20% to 60% by weight based on the total weight of the
detergent composition such as, for example, 25% to 55% by weight,
30% to 55% by weight, or 34% to 50% water by weight. The water
employed can be any suitable type of water known in the art such
as, for example, at least one of de-ionized water, distilled water,
reverse osmosis treated water, filtered water, sterile water, tap
water, and the like.
[0082] It is understood that a detergent composition of the present
disclosure can be made in a concentrated or diluted form.
Accordingly, the present disclosure provides examples wherein the
percentage by weight, based on the total weight of the composition,
of various components of the detergent composition are at
relatively high values, and examples wherein the percentage by
weight, based on the total weight of the composition, of various
components of the detergent composition are at relatively low
values. Compositions of relatively high and relatively low
concentrations are contemplated herein and may serve certain
intended purposes.
[0083] The detergent compositions of the present disclosure can be
stored for a period of time before use in a cleaning and/or
disinfecting. After storage, the detergent compositions can
maintain an enzymatic activity of an enzyme suitable for removing
and/or eliminating debris and/or bioburden. For example, the
detergent compositions of the present disclosure can comprise a
four week storage stability at 40 degrees Celsius suitable to
maintain the enzymatic activity of the enzyme of at least 40% of an
initial enzymatic activity of the enzyme prior to storage such as,
for example, at least 50% of the initial enzymatic activity, at
least 60% of the initial enzymatic activity, at least 70% of the
initial enzymatic activity, at least 80% of the initial enzymatic
activity, at least 90% of the initial enzymatic activity, or at
least 95% of the initial enzymatic activity prior to storage. The
detergent compositions of the present disclosure can comprise a
four week storage stability at 30 degrees Celsius suitable to
maintain an enzymatic activity of the enzyme of at least 40% of an
initial enzymatic activity of the enzyme prior to storage such as,
for example, at least 50% of the initial enzymatic activity, at
least 60% of the initial enzymatic activity, at least 70% of the
initial enzymatic activity, at least 80% of the initial enzymatic
activity, at least 90% of the initial enzymatic activity, or at
least 95% of the initial enzymatic activity. The detergent
compositions of the present disclosure can comprise a four week
storage stability at 25 degrees Celsius suitable to maintain an
enzymatic activity of the enzyme of at least 40% of an initial
enzymatic activity of the enzyme in the enzyme prior to storage
such as, for example, at least 50% of the initial enzymatic
activity, at least 60% of the initial enzymatic activity, at least
70% of the initial enzymatic activity, at least 80% of the initial
enzymatic activity, at least 90% of the initial enzymatic activity,
or at least 95% of the initial enzymatic activity.
[0084] The present disclosure also provides methods of making a
detergent composition. The components of the detergent composition
set forth herein can be combined in any suitable manner and in the
various amounts set forth herein. For example, the antimicrobial
agent, the hydrotrope, and the enzyme can be combined in any manner
in the amounts set forth herein to form the detergent compositions
of the present disclosure. For example, based on the total weight
of the detergent composition, at least 10% by weight of water, at
least 0.001% by weight of the antimicrobial agent, at least 0.01%
by weight of the hydrotrope, and the enzyme can be combined to form
the detergent composition. The components of the detergent
compositions of the present disclosure can be combined in any
order. For example, combining the water, antimicrobial agent, and
the hydrotrope can occur prior to adding the enzyme. Also, optional
components provided herein can be added to the detergent
compositions of the present disclosure. For example, at least one
of the buffer, the chelating agent, the solvent, the non-ionic
surfactant, and the salt described herein can be added to the
detergent compositions of the present disclosure. The pH of the
detergent composition can be adjusted to a pH suitable to maintain
enzymatic activity of an enzyme. The pH can be adjusted prior to
adding the enzyme. When a dry, powdered detergent composition is
desired, dry, powered components can be mixed together to form a
powdered mixture and then surfactants can be mixed with, such as
sprayed onto (e.g., in a liquid form), the powdered mixture. The
powdered mixture can be mixed until a desired homogeneity is
achieved.
[0085] The components of the detergent compositions of the present
disclosure can be combined in various orders. For example, the
components of the detergent composition can be combined by adding
each component one at a time, adding multiple components in a
single step, or adding a portion of a component at multiple
addition stages. For example, based on the total weight of the
detergent composition, at least 10% by weight of water can be
combined with at least 0.001% by weight of the antimicrobial agent,
followed by at least 0.01% by weight of the hydrotrope. The buffer,
if present, can then be added, followed by the addition of the
enzyme. Thereafter, the pH can be adjusted to a level appropriate
for the enzyme to retain enzymatic activity. Alternatively, the
enzyme can be added after the pH of the detergent composition has
been adjusted.
[0086] The water, the antimicrobial agent, the hydrotrope, the
non-ionic surfactant, and the buffer can each be added in a single
portion or in multiple portions. For example, a first portion of
the buffer can be added to the water to form a first composition,
the solvent can be added to the first composition to form a second
composition, and the remaining portion of the buffer can be added
to the second composition to form a third composition. Then, the
third composition can be combined with the antimicrobial agent, the
hydrotrope, and the enzyme, in a single step, in series, or in some
other combination, to form the detergent composition of the present
disclosure. The enzyme can be the final component added.
[0087] A method for cleaning all or a portion of an object is also
provided herein. For example, the object can be a reusable medical
device such as, for example, an endoscope, and the detergent
composition can be used to clean and disinfect the endoscope. The
detergent composition of the present disclosure can clean and/or
disinfect the object by removing debris and/or bioburden, from the
object. The method for cleaning the object can comprise applying
the detergent composition of the present disclosure to the object.
As used herein, "applying" is meant to include all or a portion of
the object, including one or more surfaces of the object, whether
the surface of the object is an exterior surface, an interior
surface, or a cavity of the object. As set forth above, the
detergent composition can comprise the various components and
amounts set forth herein. For example, based on the total weight of
the detergent composition, the detergent composition can comprise
at least 0.001% by weight of the antimicrobial agent, at least
0.01% by weight of the hydrotrope, and the enzyme. Application of
the detergent composition of the present disclosure thereby cleans
the object. The detergent composition can be applied to the object
by any suitable means. For example, applying the detergent
composition of the present disclosure to the object can comprise at
least one of depositing, scrubbing, spraying, rolling, submerging,
and/or agitating the detergent composition over, onto, or inside
the object.
[0088] Before or during applying the detergent composition of the
present disclosure to the object, the detergent composition can be
diluted, if necessary, to a lower concentration. The dilution can
be by a factor of at least 5-fold, at least 10-fold, at least
20-fold, at least 50-fold, at least 100-fold, at least 200-fold, or
any factor appropriate to achieve treatment of the object and/or
conserve the detergent composition. The diluting can be automated
or manual. The diluent can comprise or consist of, for example,
water in any type as already described herein.
[0089] The detergent composition can be applied to the object
manually or automatically (e.g., mechanically) to clean and/or
disinfect the object. After application, the detergent composition
can be immediately removed from, or be allowed to remain on, the
object for a period of time. The detergent composition and any
remaining debris or bioburden can be removed from the object by
wiping, rinsing, drying, or combinations thereof.
[0090] The cleaning and/or disinfecting can occur in a treatment
system such as, for example, an automated endoscope re-processor.
Referring to FIG. 1, the treatment system 100 can comprise a
chamber 102 including a basin 104 in fluid communication with a
reservoir 106. The chamber 102 may be any suitable size and
configuration to receive the object (not shown), and can be
suitable to perform a treatment process on the object. The chamber
102 can be at least one of a cleaning chamber and/or a disinfection
chamber. The object can comprise an endoscope. The treatment system
100 can comprise an automated endoscope re-processor.
[0091] The reservoir 106 can be any suitable size and configuration
to receive the detergent composition of the present disclosure and
can store the detergent composition until the detergent composition
can be output into the basin 104. The basin 104 can be in fluid
communication with the reservoir 106 via a treatment line 108 and
can receive detergent composition from the reservoir 106. The
treatment line 108 can receive the detergent composition from the
reservoir 106 and transport the detergent composition to the basin
104. The treatment line 108 can include at least one of a tube, a
valve, and a pump. The treatment line 108 can control the amount of
detergent composition provided to the basin 104. For example, the
detergent composition can be metered into the basin 104 by the
treatment line 108 until a select amount of detergent composition
has been provided to the basin 104. The basin 104 can be in fluid
communication with a drain line 110 to remove detergent composition
from the basin.
[0092] An object to be treated can be provided to the chamber 102
and subjected to the treatment process therein. The treatment
process can comprise providing the detergent composition to the
basin 104 and/or applying the detergent composition to the object.
For example, the detergent composition can be sprayed and/or
deposited on the object by a spray arm (not shown) in the chamber
102. Thereafter, the object can be optionally wiped, rinsed, and/or
dried and removed from the chamber 102.
[0093] Applying the detergent composition to the object can occur
at an operating temperature in a range of 15.degree. C. to
60.degree. C. such as, for example, 15.degree. C. to 50.degree. C.,
30.degree. C. to 50.degree. C., or 43.degree. C. to 48.degree. C.
The operating temperature can be achieved through automation, such
as in an automated endoscope re-processor, a similar machine, or by
heating the detergent composition independent of an automated
endoscope re-processor.
EXAMPLES
[0094] The present disclosure will be more fully understood by
reference to the following examples, which provide illustrative,
non-limiting aspects of the invention. The examples describe the
making of detergent compositions and use thereof in cleaning and/or
disinfecting.
Example 1
[0095] Detergent compositions F1-F6*, provided below, were
manufactured as shown in Table 1. Glycine and calcium chloride were
obtained from VWR International, Randor, Pa. Sodium hydroxide and
2-ethoxyethanol were obtained from Sigma-Aldrich, St. Louis, Mo.
Propylene glycol was obtained from Ward's Science, Rochester, N.Y.
Savinase.RTM. everis, Stainzyme.RTM. plus, and Lipex.RTM. everis
were obtained from Novozymes A/S, Denmark. Savinase.RTM. everis
comprises an alkaline protease having an active enzyme protein of
2.5% to 5% by weight of the Savinase.RTM. solution. Stainzyme.RTM.
plus comprises an alpha-amylase having active enzyme protein of 1%
to 2.5% by weight of the Stainzyme.RTM. solution. Lipex.RTM. everis
comprises a lipase. Savinase.RTM. everis, Stainzyme.RTM. plus, and
Lipex.RTM. everis were obtained in solution. BTC 1210.RTM. was
obtained from Stepan Company, Northfield, Ill. Trilon M.RTM. was
obtained from BASF, SE, Ludwigshafen, Germany. Dehypon.RTM. LS 54
was obtained from BASF SE, Ludwigshafen, Germany.
[0096] 1.6 kilograms of each detergent composition F1-F6* was
prepared. For the preparation of each detergent compositions
F1-F6*, glycine was added to de-ionized water and then sodium
hydroxide and Trilon M.RTM. were added in order respectively. The
pH of the detergent composition was adjusted and/or maintained by
addition of the glycine and the sodium hydroxide to the detergent
composition. If used (e.g., detergent compositions F1, F3, F4*, and
F6*), 2-ethoxyethanol was added after the addition of the Trilon
M.RTM.. Similarly, if used (detergent compositions F2, F3, F5*, and
F6*), propylene glycol was added after the addition of the Trilon
M.RTM. and the optional addition of the 2-ethoxyethanol. Then, for
detergent compositions F4*-F6*, BTC.RTM. 1210 was added.
Thereafter, for detergent compositions F1-F6*, Dehypon.RTM. LS 54
and Calcium chloride were added in order respectively. The enzyme
solutions, Savinase.RTM. everis, Stainzyme.RTM. plus, and
Lipex.RTM. everis were added last.
[0097] As shown in Table 1, detergent compositions F1 and F4* are
similar except that F4* comprises BTC.RTM. 1210. Detergent
compositions F2 and F5* are similar except that F5* comprises
BTC.RTM. 1210. Detergent compositions F3 and F6* are similar except
that F6* comprises BTC.RTM. 1210.
TABLE-US-00001 TABLE 1 Weight percentage (wt. %) of each component
of the detergent compositions F1-F6* based on the total weight of
the composition. Detergent Composition Component F1 F4* F2 F5* F3
F6* De-ionized 49.84 44.842 49.842 44.842 39.842 34.842 Water (wt.
%) Glycine (wt. %) 2 2 2 2 2 2 Sodium 0.098 0.098 0.098 0.098 0.098
0.098 Hydroxide (wt. %) Trilon M .RTM. 1 1 1 1 1 1 (wt. %)
2-Ethoxyethanol 25 25 -- -- 25 25 (wt. %) Propylene glycol -- -- 25
25 10 10 (wt. %) BTC .RTM. 1210 -- 5 -- 5 -- 5 (wt. %) Dehypon
.RTM. 2 2 2 2 2 2 LS 54 (wt. %) Calcium chloride 0.06 0.06 0.06
0.06 0.06 0.06 (wt. %) Savinase .RTM. 10 10 10 10 10 10 everis (wt.
%) Stainzyme .RTM. 5 5 5 5 5 5 plus (wt. %) Lipex .RTM. everis 5 5
5 5 5 5 (wt. %) Final pH 8.99 8.77 8.95 8.86 8.98 8.85 *These
detergent compositions contain a quaternary ammonium compound.
Example 2
[0098] To determine the effects of quaternary ammonium compound
presence on enzymatic activity, the detergent compositions F1-F6*
were stored at 25 degrees Celsius, 30 degrees Celsius, 40 degrees
Celsius, and 50 degrees Celsius in duplicate for 4 weeks. At two
weeks and four weeks, aliquots of the stored detergent compositions
F1-F6* were sampled and tested for the enzymatic activity against a
standard substrate. Enzyme activities of each aliquot were compared
to initial activities of the detergent compositions F1-F6* before
storage. Surprisingly, as illustrated in Tables 2-7 and described
herein, the detergent compositions F4*-F6*maintained an amount of
enzymatic activity suitable to remove and/or eliminate debris
and/or bioburden from an object after storage.
[0099] Results for Savinase.RTM. ever is are shown in Table 2 which
shows enzymatic activity data after 2 weeks of storage and Table 3
which shows enzymatic activity data after 4 weeks of storage.
Detergent compositions F4*-F6* maintained an amount of
Savinase.RTM. enzyme activity suitable to clean and/or disinfect an
object at after storage for 4 weeks at 25 degrees Celsius, 30
degrees Celsius, and 40 degrees Celsius. Detergent composition F5*
had an enhanced Savinase.RTM. storage stability compared to
detergent composition F2 after storage for 4 weeks at 25 degrees
Celsius, 30 degrees Celsius, and 40 degrees Celsius.
TABLE-US-00002 TABLE 2 Savinase .RTM. enzymatic activity as a
percentage of initial enzymatic activity after 2 weeks of storage
Storage Detergent Composition Temperature F1 F4* F2 F5* F3 F6*
25.degree. C. 100 89 92 96 96 92 95 89 89 96 97 96 30.degree. C. 87
73 86 97 85 80 86 72 88 94 84 84 40.degree. C. 55 39 76 77 48 41 55
42 70 79 49 40
TABLE-US-00003 TABLE 3 Savinase .RTM. enzymatic activity as a
percentage of initial enzymatic activity after 4 weeks of storage
Storage Detergent Composition Temperature F1 F4* F2 F5* F3 F6*
25.degree. C. 93 84 90 97 94 90 92 82 89 100 92 97 30.degree. C. 74
63 87 94 71 73 79 64 87 100 71 72 40.degree. C. 37 23 56 70 37 21
37 23 56 68 34 22
[0100] Results for Stainzyme.RTM. Plus are shown in Table 4 which
shows enzymatic activity data after 2 weeks of storage and Table 5
which shows enzymatic activity data after 4 weeks of storage.
Detergent compositions F4*-F6* maintained an amount of
Stainzyme.RTM. Plus enzymatic activity suitable to clean and/or
disinfect an object after 4 weeks of storage at 25 degrees Celsius,
30 degrees Celsius, and 40 degrees Celsius. Detergent composition
F5* had an enhanced Stainzyme.RTM. Plus storage stability compared
to detergent composition F2 after 4 weeks of storage at 40 degrees
Celsius. Detergent composition F6* had an enhanced Stainzyme.RTM.
Plus storage stability compared to detergent composition F3 after 4
weeks of storage at 25 degrees Celsius, and 30 degrees Celsius, 40
degrees Celsius.
TABLE-US-00004 TABLE 4 Stainzyme .RTM. Plus enzymatic activity as a
percentage of initial enzymatic activity at 2 weeks Storage
Detergent Composition Temperature F1 F4* F2 F5* F3 F6* 25.degree.
C. 90 98 98 85 96 100 89 97 95 89 96 100 30.degree. C. 85 96 91 87
94 100 84 97 93 84 94 100 40.degree. C. 68 80 76 82 78 99 67 84 78
80 76 97 50.degree. C. 63 74 50 73 16 86 62 74 50 71 16 85
TABLE-US-00005 TABLE 5 Stainzyme .RTM. Plus enzymatic activity as a
percentage of initial enzymatic activity at 4 weeks Storage
Detergent Composition Temperature F1 F4* F2 F5* F3 F6* 25.degree.
C. 85 96 92 88 93 100 82 95 93 88 93 100 30.degree. C. 76 93 83 84
86 100 75 93 83 84 85 99 40.degree. C. 48 67 58 71 14 81 49 65 61
72 14 83 50.degree. C. 47 60 40 61 14 72 47 60 38 61 14 72
Example 3
[0101] Detergent compositions A1-A4, provided below, were
manufactured as shown in Table 6. Glycine and calcium chloride were
obtained from VWR International, Randor, Pa. Sodium hydroxide was
obtained from Sigma-Aldrich, St. Louis, Mo. Propylene glycol was
obtained from Ward's Science, Rochester, N.Y. Ethylene glycol was
obtained from VWR International, Randor, Pa. Colatrope.RTM. OD was
obtained from Colonial Chemical, Inc., South Pittsburgh, Tenn.,
USA. Dehypon.RTM. LS 54, Dehypon.RTM. LS 36, Pluronic.RTM. L62, and
Trilon M.RTM. were obtained from BASF SE, Ludwigshafen, Germany.
Baypure.RTM. CX100 was obtained from Lanxess AG, Cologne, Germany.
Dissolvine.RTM. GLS 47 was obtained from Akzo Nobel N.V.,
Amsterdam, Netherlands.
[0102] 1 kilogram of each detergent composition A1-A4 was
prepared.
TABLE-US-00006 TABLE 6 Weight percentage (wt. %) of each component
of the detergent compositions A1-A4 based on the total weight of
the composition Component Detergent Compositions Component Type A0
A1 A2 A3 A4 De-ionized Solvent Qs to Qs to Qs to Qs to Qs to water
100 100 100 100 100 Propylene Solvent 20 10 12 15 0 glycol Ethylene
Solvent 0 0 0 0 15 glycol Sodium Hydrotrope 0 10 0 0 12 xylene
sulfonate Sodium Hydrotrope 0 0 0 10 0 cumene sulfonate Colatrope
.RTM. Hydrotrope 15 0 15 0 0 OD Calcium Enzyme 0.05 0.03 0.1 0.2
0.05 chloride stabilizer Sodium Enzyme 1 0.5 1.5 3 2 citrate
stabilizer Sodium Chelating 1 2 0 0 0.7 gluconate agent Trilon
.RTM. M Chelating 0 0 1 0 0 agent Baypure .RTM. Chelating 0 0 0 2 0
CX100 agent Dissolvine .RTM. Chelating 1 0 0 0 1.5 GLS 47 agent
Dehypon .RTM. Nonionic 3 5 0 0 4 LS 54 surfactant Dehypon .RTM.
Nonionic 0 0 2 0 1 LS 36 surfactant Pluronic .RTM. Nonionic 0 0 0 3
0.5 L62 surfactant Glycine pH buffer 1.2 1 1.2 0.8 2 Amplify
Amylase 3 1 2 10 8 100L enzyme Evens DUO Protease 5 1 1.5 7 15 100L
enzyme Chloro- Antimicrobial 5 2 3 7 9 hexidine agent (20% w/w
solution in water) Sodium pH adjuster Up to Up to Up to Up to Up to
hydroxide pH = pH = pH = pH = pH = 9 8 9 10 11 pH 9 8 9 10 11
Example 4
[0103] To determine the cleaning efficacy of detergent compositions
of the present disclosure, formulation A0 according to the present
disclosure and as described in Example 3, was compared to
commercially available detergent compositions, C1-C6, which are not
according to the present disclosure. Each detergent composition (A0
and C1-C6) was tested with and without addition of an antimicrobial
agent to control for the presence of an antimicrobial agent, which
is not necessarily present in the commercially available detergent
compositions. For all detergent compositions the antimicrobial
agent was 5% by weight chlorhexidine gluconate (abbreviated as
CHG), based on the total weight of the composition.
[0104] Detergent composition C1 is a commercially available neutral
detergent composition comprising nonionic surfactants and
triethanolamine. Detergent composition C2 is a commercially
available enzymatic detergent composition comprising protease,
amylase, and lipase enzymes. Detergent composition C3 is a
commercially available enzymatic detergent composition comprising
subtilisin protease. Detergent composition C4 is a commercially
available detergent composition comprising an antimicrobial agent
(a biguanide). Detergent composition C5 is a commercially available
enzymatic detergent comprising subtilisins (protease enzyme).
Detergent composition C6 is a commercially available dual enzymatic
detergent comprising proteinase subtilisin and subtilisin (protease
enzyme).
[0105] Cleaning efficacy was tested using stainless steel and
plastic coupons. The stainless steel coupons were Tosi.RTM. coupons
available from Healthmark Industries Company, Inc., Fraser Mich.,
USA. Each Tosi.RTM. coupon comprises a stainless steel plate
treated with simulated blood test soil to create a surface
appropriate to test for cleaning activity. The simulated blood
comprises blood proteins in a sodium chloride and calcium chloride
solution. The plastic coupons were Verify.RTM. All Clean coupons
available from STEMS Corporation Mentor, Ohio, USA. Each
Verify.RTM. All Clean coupon comprises a plastic plate treated with
a test soil comprising proteins, lipids, and polysaccharides. Thus,
testing was conducted for cleaning of both metal and plastic
surfaces, with and without antimicrobial agent.
[0106] The following procedure was used to perform the cleaning
tests. First, each detergent composition was diluted with 200 PPM
hard water to the recommended concentration in a first beaker. For
detergent composition A0, the dilution factor was 1:100. Then, the
resulting diluted detergent composition was heated to 45.degree. C.
Then, a plastic and stainless steel coupon were immersed in the
beaker for 10 min. After 10 min, each coupon was removed, and
rinsed with de-ionized water. Then, each coupon was dried at room
temperature overnight and examined and photographed for cleaning
efficacy.
[0107] Results are shown in FIGS. 2-8. Generally, each Figure has a
panel A, B, C, and D, with one photograph in each panel. Panel A of
each Figure shows a stainless steel coupon treated with a diluted
detergent composition to which 5% CHG was added. Panel B of each
Figure shows a plastic coupon treated with a diluted detergent
composition to which 5% CHG was added. Panel C of each Figure shows
a stainless steel coupon treated with a diluted detergent
composition to which no CHG was added. Panel D of each Figure shows
a plastic coupon treated with a diluted detergent composition to
which no CHG was added.
[0108] FIG. 2 shows photographs of stainless steel and plastic
coupons that were treated with diluted detergent composition A0.
Panel 2A shows a stainless steel coupon treated with diluted
detergent composition A0 to which 5% CHG was added. Panel 2B shows
a plastic coupon treated with diluted detergent composition A0 to
which 5% CHG was added. Panel 2C shows a stainless steel coupon
treated with diluted detergent composition A0 to which no CHG was
added. Panel 2D shows a plastic coupon treated with diluted
detergent composition A0 to which no CHG was added.
[0109] FIG. 3 shows photographs of stainless steel and plastic
coupons that were treated with diluted detergent composition C1.
Panel 3A shows a stainless steel coupon treated with diluted
detergent composition C1 to which 5% CHG was added. Panel 3B shows
a plastic coupon treated with diluted detergent composition C1 to
which 5% CHG was added. Panel 3C shows a stainless steel coupon
treated with diluted detergent composition C1 to which no CHG was
added. Panel 3D shows a plastic coupon treated with diluted
detergent composition C1 to which no CHG was added. For all panels
A-D of FIG. 3, comparison to the corresponding panels of FIG. 2
shows that cleaning efficacy is increased in detergent compositions
of the present disclosure, based on increased removal of test soil
in FIG. 2.
[0110] FIG. 4 shows photographs of stainless steel and plastic
coupons that were treated with diluted detergent composition C2.
Data for panels 4A and B was not obtainable because the 5% CHG that
was added to detergent composition C2 was insoluble. Panel 4C shows
a stainless steel coupon treated with diluted detergent composition
C2 to which no CHG was added. Panel 4D shows a plastic coupon
treated with diluted detergent composition C2 to which no CHG was
added. For panels C and D of FIG. 4, comparison to the
corresponding panels of FIG. 2 shows that cleaning efficacy is
increased in detergent compositions of the present disclosure,
based on increased removal of test soil in FIG. 2.
[0111] FIG. 5 shows photographs of stainless steel and plastic
coupons that were treated with diluted detergent composition C3.
Data for panels 5A and B was not obtainable because the 5% CHG that
was added to detergent composition C3 was insoluble. Panel 5C shows
a stainless steel coupon treated with diluted detergent composition
C3 to which no CHG was added. Panel 5D shows a plastic coupon
treated with diluted detergent composition C3 to which no CHG was
added. For panels C and D of FIG. 5, comparison to the
corresponding panels of FIG. 2 shows that cleaning efficacy is
increased in detergent compositions of the present disclosure,
based on increased removal of test soil in FIG. 2.
[0112] FIG. 6 shows photographs of stainless steel and plastic
coupons that were treated with diluted detergent composition C4.
Panel 6A shows a stainless steel coupon treated with diluted
detergent composition C4 to which 5% CHG was added. Panel 6B shows
a plastic coupon treated with diluted detergent composition C4 to
which 5% CHG was added. Panel 6C shows a stainless steel coupon
treated with diluted detergent composition C4 to which no CHG was
added. Panel 6D shows a plastic coupon treated with diluted
detergent composition C4 to which no CHG was added. For all panels
A-D of FIG. 6, comparison to the corresponding panels of FIG. 2
shows that cleaning efficacy is increased in detergent compositions
of the present disclosure, based on increased removal of test soil
in FIG. 2.
[0113] FIG. 7 shows photographs of stainless steel and plastic
coupons that were treated with diluted detergent composition C5.
Panel 7A shows a stainless steel coupon treated with diluted
detergent composition C5 to which 5% CHG was added. Panel 7B shows
a plastic coupon treated with diluted detergent composition C5 to
which 5% CHG was added. Panel 7C shows a stainless steel coupon
treated with diluted detergent composition C5 to which no CHG was
added. Panel 7D shows a plastic coupon treated with diluted
detergent composition C5 to which no CHG was added. For all panels
A-D of FIG. 7, comparison to the corresponding panels of FIG. 2
shows that cleaning efficacy is increased in detergent compositions
of the present disclosure, based on increased removal of test soil
in FIG. 2.
[0114] FIG. 8 shows photographs of stainless steel and plastic
coupons that were treated with diluted detergent composition C6.
Data for panels 8A and B was not obtainable because the 5% CHG that
was added to detergent composition C6 was insoluble. Panel 8C shows
a stainless steel coupon treated with diluted detergent composition
C6 to which no CHG was added. Panel 8D shows a plastic coupon
treated with diluted detergent composition C6 to which no CHG was
added. For panels C and D of FIG. 8, comparison to the
corresponding panels of FIG. 2 shows that cleaning efficacy is
increased in detergent compositions of the present disclosure,
based on increased removal of test soil in FIG. 2.
Example 5
[0115] To determine the cleaning and disinfection efficacy of
detergent compositions of the present disclosure, formulation A0,
according to the present disclosure and as described in Example 3,
was compared to commercially available detergent composition C2
which is not according to the present disclosure. Each detergent
composition (A0 and C2) was tested for the ability to remove
bacterial biofilms. This was measured, first, based on the ability
of treatment with the detergent composition to reduce bacterial
presence and, second, based on the ability of treatment with the
detergent composition to reduce protein content of the biofilm.
[0116] Biofilms comprising Pseudomonas aeruginosa were grown in the
lumen of a test object according to the ISO/TS 15883-5 Annex F
method. 8 test objects comprising P. aeruginosa biofilm were
treated using an automated endoscope reprocessor. The treatments
were performed in an automated endoscope repressor at 45.degree. C.
and at an exposure time of 8 min. In 4 of the treatments a test
object was treated with diluted detergent composition AX, at a
dilution factor of 1:100. In the other 4 treatments, a test object
was treated with diluted detergent composition C2, at a dilution
factor of 0.8:100, as recommended by the manufacturer. Subsequent
to the treatment, test objects were analyzed for colony forming
units (CFUs) and for total protein utilizing a BCA protein assay.
These values were compared to those obtained for an untreated test
object comprising the biofilm to determine fold-reduction of
bacteria and percent reduction of total protein.
[0117] Results for reduction of bacteria are displayed in Table 7.
In Replicate 1, treatment of a test object with diluted detergent
composition A0 resulted in a Log.sub.10-fold reduction of bacteria
of 6.98 (e.g., a reduction of 10.sup.6.98-fold). In Replicate 1,
treatment of a test object with diluted detergent composition C2
resulted in a Log.sub.10-fold reduction of bacteria of 2.64 (e.g.,
a reduction of 10.sup.2.64-fold). In Replicate 2, treatment of a
test object with diluted detergent composition A0 resulted in a
Log.sub.10-fold reduction of bacteria of 6.85 (e.g., a reduction of
10.sup.6.85-fold). In Replicate 2, treatment of a test object with
diluted detergent composition C2 resulted in a Log.sub.10-fold
reduction of bacteria of 4.89 (e.g., a reduction of
10.sup.4-89-fold). In Replicate 3, treatment of a test object with
diluted detergent composition A0 resulted in a Log.sub.10-fold
reduction of bacteria of 6.02 (e.g., a reduction of
10.sup.6.02-fold). In Replicate 3, treatment of a test object with
diluted detergent composition C2 resulted in a Log.sub.10-fold
reduction of bacteria of 1.38 (e.g., a reduction of
10.sup.1.38-fold). In Replicate 4, treatment of a test object with
diluted detergent composition A0 resulted in a Log.sub.10-fold
reduction of bacteria of 6.22 (e.g., a reduction of
10.sup.6.22-fold). In Replicate 4, treatment of a test object with
diluted detergent composition C2 resulted in a Log.sub.10-fold
reduction of bacteria of 3.56 (e.g., a reduction of
10.sup.3.56-fold).
[0118] These results indicate that cleaning and disinfecting
efficacy is increased in detergent compositions of the present
disclosure, based on the increased removal of bacteria demonstrated
by diluted detergent composition A0 in Table 7, compared to diluted
detergent composition C2.
TABLE-US-00007 TABLE 7 Log.sub.10-fold reduction of bacteria.
Diluted detergent Diluted detergent composition A0 (Log.sub.10-fold
composition C2 (Log.sub.10-fold change) change) Replicate 1 6.98
2.64 Replicate 2 6.85 4.89 Replicate 3 6.02 1.38 Replicate 4 6.22
3.56
[0119] Results for reduction of total protein are displayed in
Table 8. In Replicate 1, treatment of a test object with diluted
detergent composition A0 resulted in a reduction of total protein
on the test object of 95.5%. In Replicate 1, treatment of a test
object with diluted detergent composition C2 resulted in a
reduction of total protein on the test object of 72.3%. In
Replicate 2, treatment of a test object with diluted detergent
composition A0 resulted in a reduction of total protein on the test
object of 99.2%. In Replicate 2, treatment of a test object with
diluted detergent composition C2 resulted in a reduction of total
protein on the test object of 86.2%. In Replicate 3, treatment of a
test object with diluted detergent composition A0 resulted in a
reduction of total protein on the test object of 98.8%. In
Replicate 3, treatment of a test object with diluted detergent
composition C2 resulted in a reduction of total protein on the test
object of 95.8%. In Replicate 4, treatment of a test object with
diluted detergent composition A0 resulted in a reduction of total
protein on the test object of 92.4%. In Replicate 4, treatment of a
test object with diluted detergent composition C2 resulted in a
reduction of total protein on the test object of 81.2%.
[0120] In each replicate shown in Table 8, cleaning and
disinfecting efficacy was increased in detergent compositions of
the present disclosure, based on the increased removal of total
protein demonstrated by diluted detergent composition A0, compared
to diluted detergent composition C2.
TABLE-US-00008 TABLE 8 Percent reduction of protein. Diluted
detergent Diluted detergent composition A0 composition C2 Replicate
1 95.5% 72.3% Replicate 2 99.2% 86.2% Replicate 3 98.8% 95.8%
Replicate 4 92.4% 81.2%
[0121] The grammatical articles "a", "an", and "the", as used
herein, are intended to include "at least one" or "one or more",
unless otherwise indicated, even if "at least one" or "one or more"
is expressly used in certain instances. Thus, the articles are used
herein to refer to one or more than one (i.e., to "at least one")
of the grammatical objects of the article. Further, the use of a
singular noun includes the plural, and the use of a plural noun
includes the singular, unless the context of the usage requires
otherwise.
[0122] One skilled in the art will recognize that the herein
described compositions, methods, and the discussion accompanying
them are used as examples for the sake of conceptual clarity and
that various configuration modifications are contemplated.
Consequently, as used herein, the specific exemplars set forth and
the accompanying discussion are intended to be representative of
their more general classes. In general, use of any specific
exemplar is intended to be representative of its class, and the
non-inclusion of specific components (e.g., operations), devices,
and objects should not be taken limiting.
[0123] The herein described subject matter sometimes illustrates
different components contained within, or connected with, different
other components. It is to be understood that such depicted
architectures are merely exemplary, and that in fact many other
architectures may be implemented which achieve the same
functionality. In a conceptual sense, any arrangement of components
to achieve the same functionality is effectively "associated" such
that the desired functionality is achieved. Hence, any two
components herein combined to achieve a particular functionality
can be seen as "associated with" each other such that the desired
functionality is achieved, irrespective of architectures or
intermedial components. Likewise, any two components so associated
can also be viewed as being "operably connected," or "operably
coupled," to each other to achieve the desired functionality, and
any two components capable of being so associated can also be
viewed as being "operably couplable," to each other to achieve the
desired functionality. Specific examples of operably couplable
include but are not limited to physically mateable and/or
physically interacting components, and/or wirelessly interactable,
and/or wirelessly interacting components, and/or logically
interacting, and/or logically interactable components.
[0124] With respect to the appended claims, those skilled in the
art will appreciate that recited operations therein may generally
be performed in any order. Also, although various operational flows
are presented in a sequence(s), it should be understood that the
various operations may be performed in other orders than those
which are illustrated, or may be performed concurrently. Examples
of such alternate orderings may include overlapping, interleaved,
interrupted, reordered, incremental, preparatory, supplemental,
simultaneous, reverse, or other variant orderings, unless context
dictates otherwise. Furthermore, terms like "responsive to,"
"related to," or other past-tense adjectives are generally not
intended to exclude such variants, unless context dictates
otherwise.
[0125] Although various examples have been described herein, many
modifications, variations, substitutions, changes, and equivalents
to those examples may be implemented and will occur to those
skilled in the art. Also, where materials are disclosed for certain
components, other materials may be used. It is therefore to be
understood that the foregoing description and the appended claims
are intended to cover all such modifications and variations as
falling within the scope of the disclosed examples. The following
claims are intended to cover all such modification and
variations.
[0126] Any patent, publication, or other disclosure material, in
whole or in part, that is said to be incorporated by reference
herein is incorporated herein only to the extent that the
incorporated materials does not conflict with existing definitions,
statements, or other disclosure material set forth in this
disclosure. As such, and to the extent necessary, the disclosure as
explicitly set forth herein supersedes any conflicting material
incorporated herein by reference. Any material, or portion thereof,
that is said to be incorporated by reference herein, but which
conflicts with existing definitions, statements, or other
disclosure material set forth herein will only be incorporated to
the extent that no conflict arises between that incorporated
material and the existing disclosure material.
[0127] Various aspects of the invention according to the present
disclosure include, but are not limited to, the aspects listed in
the following numbered clauses. [0128] 1. A detergent composition
comprising: [0129] at least 0.001% by weight of an antimicrobial
agent, based on the total weight of the composition; [0130] an
enzyme; and [0131] at least 0.01% a hydrotrope, based on the total
weight of the composition. [0132] 2. The composition of clause 1,
wherein the hydrotrope comprises an anionic hydrotrope. [0133] 3.
The composition of clause 2, wherein the anionic hydrotrope
comprises at least one of an alkanoic acid, an aromatic sulfonic
acid, an aromatic carboxylic acid, and a salt of any thereof.
[0134] 4. The composition of clause 3, wherein the aromatic
sulfonic acid is at least one of xylene sulfonic acid, cumene
sulfonic acid, and a salt of any thereof. [0135] 5. The composition
of any one of clauses 1-4, comprising 0.1% or less by weight of a
boron-containing compound, based on the total weight of the
composition. [0136] 6. The composition of any one of clauses 1-5,
comprising either no boron-containing compound or only an
incidental amount. [0137] 7. The composition of any one of clauses
1-6, wherein the antimicrobial agent comprises at least one of a
biguanide compound and a quaternary ammonium compound. [0138] 8.
The composition of clause 7, wherein the biguanide compound
comprises at least one of chlorhexidine and a salt thereof. [0139]
9. The composition of any one of clauses 1-8, wherein the enzyme
comprises at least one of a lipase, a protease, a peptidase, an
amylase, a glycosidase, a cellulase, DNAse and a nuclease. [0140]
10. The composition of any one of clauses 1-9, wherein the
composition has a pH in a range of 6 to 11. [0141] 11. The
composition of any one of clauses 1-10, further comprising at least
one of [0142] at least 0.0001% by weight of a buffer, based on the
total weight of the composition, and [0143] at least 0.0001% by
weight of a pH adjusting agent, based on the total weight of the
composition. [0144] 12. The composition of any one of clauses 1-11,
further comprising at least 0.01% by weight of a solvent, based on
the total weight of the composition. [0145] 13. The composition of
clause 12, wherein the solvent comprises at least one of a glycol
ether, propylene glycol, ethylene glycol, methanol, ethanol,
isopropanol, and n-propanol. [0146] 14. The composition of any one
of clauses 1-13, further comprising at least one of a chelating
agent and a salt. [0147] 15. The composition of any one of clauses
1-14, further comprising at least 10% by weight of water, based on
the total weight of the composition. [0148] 16. The composition of
any one of clauses 1-15, further comprising at least 0.005% by
weight of a non-ionic surfactant, based on the total weight of the
composition. [0149] 17. The composition of clause 16, wherein the
non-ionic surfactant is low foam. [0150] 18. A method of making a
detergent composition, the method comprising: [0151] combining,
based on the total weight of the composition, [0152] at least
0.001% by weight of an antimicrobial agent, [0153] at least 0.01%
by weight of a hydrotrope; and [0154] an enzyme. [0155] 19. The
method of clause 18, further comprising adjusting a pH of the
detergent composition prior to the adding the enzyme. [0156] 20.
The method of any one of clauses 18-19, further comprising adding
at least one of a buffer, a chelating agent, a solvent, water, a
non-ionic surfactant, and a salt. [0157] 21. A method for cleaning
an object, comprising: [0158] applying a detergent composition to
the object, the composition comprising, based on the total weight
of the composition: [0159] at least 0.001% by weight of an
antimicrobial agent, [0160] at least 0.01% by weight of a
hydrotrope, and [0161] an enzyme [0162] thereby cleaning the
object. [0163] 22. The method of clause 21, wherein the object
comprises an endoscope. [0164] 23. The method of any one of clauses
21-22, further comprising disinfecting the object. [0165] 24. The
method of any one of clauses 21-23, wherein the applying the
composition comprises utilizing an automated endoscope
re-processor. [0166] 25. The method of any one of clauses 21-24,
wherein an operating temperature is from 15.degree. C. to
60.degree. C.
[0167] In summary, numerous benefits have been described which
result from employing the concepts described herein. The foregoing
description of the one or more examples has been presented for
purposes of illustration and description. It is not intended to be
exhaustive or limiting to the precise form disclosed. Modifications
or variations are possible in light of the above teachings. The one
or more examples were chosen and described in order to illustrate
principles and practical application to thereby enable one of
ordinary skill in the art to utilize the various examples and with
various modifications as are suited to the particular use
contemplated. It is intended that the claims submitted herewith
define the overall scope.
[0168] While the present disclosure provides descriptions of
various specific aspects for the purpose of illustrating various
aspects of the present disclosure and/or its potential
applications, it is understood that variations and modifications
will occur to those skilled in the art. Accordingly, the invention
or inventions described herein should be understood to be at least
as broad as they are claimed, and not as more narrowly defined by
particular illustrative aspects provided herein.
* * * * *