Inhibition Of Bolting And Flowering Of A Beta Vulgaris Plant

Abstract

The present invention provides means for inhibiting the bolting and flowering of a Beta vulgaris plant, including an isolated nucleic acid, which can be used to produce a transgenic Beta vulgaris plant, where bolting and flowering is inhibited after vernalization. Furthermore, the invention discloses vectors, transgenic and non-transgenic, non-bolting plants and parts thereof, and methods for producing such plants.

Description

CROSS-REFERENCE TO RELATED PATENT APPLICATIONS

[0001] This application is a continuation of U.S. application Ser. No. 15/771,873, filed Apr. 27, 2018, which is a National Stage of International Application No. PCT/EP2016/076090, filed Oct. 28, 2016, which claims priority of European Patent Application No. 15003108.6, filed Oct. 30, 2015, the contents of each of which are herein fully incorporated by reference into this application.

SEQUENCE LISTING

[0002] This application contains a Sequence Listing which has been submitted in ASCII format via EFS-Web and is hereby incorporated by reference herein in its entirety. The ASCII text file was created on Apr. 17, 2018, is named SequenceListing_ST25.txt and is 246,431 bytes in size.

[0003] The present invention relates to an isolated nucleic acid for inhibiting bolting (the first visible sign of reproductive transition in beets) and flowering of a Beta vulgaris plant, as well as the use thereof, a method for producing a transgenic Beta vulgaris plant or an non-transgenic Beta vulgaris plant in which bolting and flowering is inhibited after vernalization, vectors or mobile genetic elements, as well as a transgenic or non-transgenic Beta vulgaris in which bolting and flowering is inhibited after vernalization, and seeds as well as their parts.

[0004] It is possible to use molecular biological techniques or mutagenesis techniques to genetically modify crops in order to change their properties and thus to improve them. One property of importance in the cultivation and use of biennial plants such as Beta vulgaris is that bolting and subsequent flowering requires an induction by a longer period of cold weather, as regularly occurs in temperate latitudes in winter. This transition from the vegetative to the generative phase induced by a prolonged period of low temperature is referred to as vernalization.

[0005] There are several metabolic pathways by which flowering is controlled. These include inter alia the photoperiodic metabolic pathway, an autonomous pathway, a gibberellic acid and a vernalization dependent pathway. A large number of genes involved in the regulation of flowering have been identified in recent years in model plants. In particular the control of the timing of flowering was extensively explored in the model plant Arabidopsis (Boss, P K, Bastow R M, Mylne, J S, and Dean, C. (2004) Multiple pathways in the decision to flower: enabling, promoting, and resetting, Plant Cell 16 Suppl: 18-31; He, Y. and Amasino, RM (2005) Role of chromatin modification in flowering-time control, Trends Plant Sci 10, 30-35; Baeurle, I. and Dean, C. (2006) The timing of developmental transitions in plants, Cell, 125 (4): 655-664). Primarily using Arabidopsis mutants many "early flowering" or "late-flowering" genes were identified (Gazzani S., Gendall, A R, Lister, C., and Dean , C. (2003) Analysis of the molecular basis of flowering time variation in Arabidopsis accessions, Plant Physiol 132:. 1107-1114; Geraldo, N., Baurle, I., Kidou, S., Hu, X., and Dean , C. (2009), FRIGIDA Delays Flowering in Arabidopsis via a Mechanism Involving Cotranscriptional Direct Interaction with the Nuclear Cap-Binding Complex, Plant Physiology, Jul. 1, 2009; 150 (3): 1611-1618; Michaels S D, Amasino, R M (2001) Loss of FLOWERING LOCUS C activity eliminates the late-flowering phenotype of FRIGIDA and autonomous pathway mutations but not responsiveness to vernalization, Plant Cell 13: 935-942; Yalovsky, Shaul, et al. "Prenylation of the floral transcription factor APETALA1 modulates its function." The Plant Cell 12.8 (2000): 1257-1266; Gu, Qing, et al. "The FRUITFULL MADS-box gene mediates cell differentiation during Arabidopsis fruit development." Development 125.8 (1998): 1509-1517).

[0006] In Beta vulgaris so far only very few genes have been characterized in detail. Therein it has been shown that for instances the gene BvFLC is not a key control gene for flowering or vernalization in Beta vulgaris (Reeves, P A, He Y, Schmitz R J, Amasino R M, Panella, L W, Richards C M (2007), Evolutionary FLOWERING LOCUS conservation of the C-mediated vernalization response: evidence from the sugar beet (Beta vulgaris), Genetics 176 (1): 295-307; Chia, T. Y. P., Mueller, A., Young, C., and Mutasa-Goettgens, E. S. (2008), Sugar beet contains a large CONSTANS-LIKE gene family including a CO homolog that is independent of the early-bolting (B) gene locus, J Exp Bot 59 (10): 2735-2748). In 2011 Kraus et al. showed that BvVil1 seems to have a more crucial role in controlling bolting activity after vernalization (WO 2011/032537).

[0007] Bolting and flowering of Beta vulgaris plants is undesirable, since in the case of Beta vulgaris it is not the seeds or fruits, but rather the underground part of the plant, the storage root, that is used, and the energy stored in the root would be consumed during the bolting and flowering of the plant. Moreover, in some plants, which are called "bolters", an unwanted emergence of shoots occurs in the first year of growing, which is very disadvantageous for harvesting and processing.

[0008] It is thus the object of the present invention to provide means to make it possible to inhibit bolting and/or flowering of Beta vulgaris plants, and even to completely prevent this.

[0009] According to the invention the problem is solved by means of an isolated nucleic acid for the inhibition of bolting and flowering of a Beta vulgaris plant, wherein the nucleic acid comprises at least one nucleotide sequence which a) exhibits a sequence or partial sequence of SEQ ID NO: 1 or 2, or b) is complementary to a sequence or partial sequence of a), or c) exhibits in the antisense direction a sequence or partial sequence of a) or b), or d) is a homolog to a sequence or partial sequence of a), ore) at least 80% or 85%, preferably at least 90%, 95%, 96%, 97%, 98% or 99%, or more preferably at least 99.5%, 99, 6%, 99.7%, 99.8% or 99.9% identical to a sequence or partial sequence of a), or f) encodes a protein or a part of the protein with the amino acid sequence of SEQ ID NO: 5, or g) encodes a protein with an amino acid sequence of Beta vulgaris which is a homolog to the sequence of f), or h) hybridizes under stringent conditions with a sequence or partial sequence of a), b) or c) and/or at least one nucleotide sequence which A) exhibits a sequence or partial sequence of SEQ ID NO: 3 or 4, or B) is complementary to a sequence or partial sequence of A), or C) exhibits in the antisense direction a sequence or partial sequence of A) or B), or D) is a homolog to a sequence or partial sequence of A), or E) at least 80% or 85%, preferably at least 90%, 95%, 96%, 97%, 98% or 99%, or more preferably at least 99.5%, 99, 6%, 99.7%, 99.8% or 99.9% identical to a sequence or partial sequence of A), or F) encodes a protein or a part of the protein with the amino acid sequence of SEQ ID NO: 6, or G) encodes a protein with an amino acid sequence of Beta vulgaris which is a homolog to the sequence of F), or H) hybridizes under stringent conditions with a sequence or partial sequence of A), B) or C).

[0010] The inventive nucleic acid can be used, for example by the RNA interference (RNAi) approach or micro-RNA (miRNA) interference approach (Fire, A, Xu, S, Montgomery, M, Kostas, S, Driver, S, Mello, C. (1998). Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans, Nature 391 (6669): 806-811) to inhibit bolting and flowering of Beta vulgaris, and in particular, if possible, to completely prevent bolting and flowering, for example by inhibiting genes that encoded flowering inducers such as FT, Co, or VIN3.

[0011] The nucleic acid is characterized especially by the fact that transgenic or non transgenic plants, in particular Beta vulgaris plants, with special characteristics can be produced with it: In beneficial manner they can be used for example for the following purposes or with the following benefits:

[0012] Production of non-shoot emergent, non-flowering Beta vulgaris plants

[0013] Production of a Beta vulgaris plant as winter beet

[0014] Production of a Beta vulgaris plant as spring beet

[0015] Increasing the biomass of the Beta vulgaris plant

[0016] Increasing the sugar yield

[0017] Avoiding Beta vulgaris bolters

[0018] Extension of the Beta vulgaris harvesting campaign

[0019] Avoidance of losses in Beta vulgaris storage material

[0020] Utilization of the higher humidity in the fall

[0021] Covering of soil and use of the stored nitrogen

[0022] Protection for beneficial insects in the field

[0023] Beta vulgaris is a biennial plant. After completion of the winter, and the vernalization resulting therefrom, Beta vulgaris usually bolts and flowers in the second year. By means of the inventive nucleic acid, for example, a sequence shown in one of SEQ ID NOs: 19-23 or another novel sequence or partial sequence inserted using an RNAi or microRNA-approach, genes can be inhibited and the effects of vernalization can be inhibited or completely prevented. Mechanisms and methods for inhibiting or switching off genes are known to the person of ordinary skill in the art, for example, under the term "gene silencing" and include the already mentioned and known to those skilled in the art RNAi or micro (mi) RNA processes, but are not limited thereto. In an RNAi approach, for example, the sequences of SEQ ID NO: 19 to SEQ ID NO: 23 can, by molecular biology techniques known to the person skilled in the art, be introduced into a Beta vulgaris cell in the antisense orientation and under control of a suitable promoter be expressed there.

[0024] In accordance with the present invention, the bolting and flowering of the plant can be completely eliminated. Seed of Beta vulgaris can be sown earlier, which ultimately leads to a longer growing season and thus leads to a higher biomass and a higher sugar yield. In combination with cold tolerance, sugar beets, for example, can be grown as so-called winter beets. In the case of seeding of sugar beets in August, in the following spring they can already be harvested as spring beets. This allows the farmer an additional crop rotation. By using the nucleic acid according to the invention, even in the case of prolonged cold spells on the field after sowing, there is no longer increased formation of bolters. Even the normal sugar beet bolters previously observed without prolonged cold spells can be prevented or at least significantly reduced. Using the present invention it can not only be accomplished that bolting and the subsequent flowering of sugar beet after an initial vernalization, but, i.e. in the second year, is inhibited or prevented, but the sugar beet can also be subjected to other cold periods without vernalization effects observed.

[0025] The sugar beet cultivation is usually from April to October/November. Since not all of the harvested sugar beets can be processed at the same time, they must be stored or intermediate stored. During storage, for example in piles, large losses in storage substance (sucrose losses) occur as a result of by cleavage of sucrose into glucose and fructose. By means of the inventive nucleic acid, particularly when used in an RNAi approach, the sowing and harvest dates can be varied so that the total harvest (campaign) can be extended without loss of harvest. It can allow more sugar beets to be processed for a prolonged period with less loss of storage material.

[0026] The term " Beta vulgaris" or " Beta vulgaris plant" is understood to refer to a plant of the genus Beta vulgaris, e.g. Beta vulgaris ssp. vulgaris var altissima (sugar beet in the narrow sense), Beta vulgaris ssp. maritima (sea beet), Beta vurlgaris ssp. vulgaris var vulgaris (Mangold beet), Beta vulgaris ssp. vulgaris var conditiva (red beetroot/beet), Beta vulgaris ssp. crassa vulgaris var/alba (fodder beet).

[0027] The term "plant" according to the present invention includes whole plants or parts of such a whole plant. Whole plants preferably are seed plants, or a crop. "Parts of a plant" are e.g. shoot vegetative organs/structures, e.g., leaves, stems and tubers; roots, flowers and floral organs/structures, e.g. bracts, sepals, petals, stamens, carpels, anthers and ovules; seed, including embryo, endosperm, and seed coat; fruit and the mature ovary; plant tissue, e.g. vascular tissue, ground tissue, and the like; and cells, e.g. guard cells, egg cells, pollen, trichomes and the like; and progeny of the same.

[0028] An "isolated nucleic acid" is understood to be a nucleic acid extracted from its natural or original environment. The term also includes a synthetic manufactured nucleic acid.

[0029] An "inhibition of bolting and flowering" of a Beta vulgaris plant refers to a reduction in the proportion of bolting and possibly flower forming Beta vulgaris plants in comparison to a non-inventively modified Beta vulgaris plant of the same subspecies or variety in a comparable stage of development, particularly in the second year after passing through a corresponding cold period, i.e. after vernalization. In particular, the term encompasses a reduction of proportion of bolters to not more than 80%, preferably not more than 70%, 60%, 50%, 40%, 30%, 20% or 10%, more preferably not more than 5%, 4%, 3%, 2% , 1%, 0.5%, 0.4%, 0.3%, 0.2% or 0.1% of the percentage of bolting compared to control plants not according to the invention. "Control plants" are preferably plants of the same variety, but they are not modified according to the present invention, and exhibit for example a proportion of bolters of at most 0.01%. The term "suppression" or "complete suppression" of bolting and flowering is understood to mean inhibition of at least 99%, preferably at least 99.5%, more preferably at least 99.8%, or at least 99.9%, that is, a reduction of the proportion of bolters to not more than 1%, not more than 0.5%, not more than 0.2% or not more than 0.1%, especially in the second year after vernalization, compared to a non-inventively modified Beta vulgaris plant, for example, a bolting percentage of maximal 0.01%. The term of inhibition or the suppression of bolting and flowering comprises mainly the inhibition/suppression of bolting, regardless of whether it comes to a flowering of the plant or not.

[0030] The term "transgenic", "transgene" or "heterologous" as used herein means genetically modified. The term includes also the case that a species-specific nucleic acid in a form, arrangement or quantity is introduced into a plant cell where the nucleic acid does not occur naturally in the cell. If the gene, coding sequence or the regulatory element may be one normally found in the cell, it is called `autologous` or `endogenous`. A `heterologous` gene, coding sequence or regulatory element may also be autologous to the cell but is, however, arranged in an order and/or orientation or in a genomic position or environment not normally found or occurring in the cell in which it is introduced.

[0031] The term "homology" refers to identities or similarities in the nucleotide sequence of two nucleic acid molecules or the amino acid sequence of two proteins or peptides. The presence of homology between two nucleic acids or proteins can be detected by comparing each position in one sequence with the equivalent position in the other sequence and determining whether identical or similar residues are present here. Two compared sequences are homologous if there is a particular minimum level of identical or similar nucleotides. "Identical" means that when comparing two sequences at equivalent positions there is the same nucleotide or the same amino acid. It may be necessary to take into account gaps in sequence to achieve the best possible alignment comparison of sequences. Similar nucleotides/amino acids are non-identical nucleotides/amino acids with the same or equivalent chemical and physical properties. Exchanging a nucleotide (an amino acid) with a different nucleotide (another amino acid) with the same or equivalent physical and chemical properties is called a "conservative exchange." Examples of chemical and physical properties of an amino acid include, for example, the hydrophobicity or charge. In the context of nucleic acids there is also understood a conservative or a similar nucleotide exchange when replacing in a coding sequence a nucleotide in a codon by another, whereby due to e.g. the degeneration of the genetic code, the same amino acid or a similar amino acid is encoded as in the equivalent codon in the compared sequence. The person skilled in the art knows which nucleotide or amino acid exchange is a conservative exchange. To determine the level of similarity or identity between two nucleic acids, a minimum length of 60 nucleotides or base pairs is assumed, preferably a minimum length of 70, 80, 90, 100, 110, 120, 140, 160, 180, 200, 250 , 300, 350 or 400 nucleotides or base pairs, more preferably the full length of the compared nucleic acids, and in the case of proteins/peptides a minimum length of 20 amino acids is assumed, preferably a minimum length of 25, 30, 35, 40, 45, 50, 60, 80, 100, 150, 200, 250 or 300 amino acids, and more preferably the full length of the compared amino acid sequences. The level of similarity ("positives") or identity of two sequences can be determined using, for example, the computer program BLAST (Altschul S. F. et al (1990), Basic Local Alignment Search Tool, J. Mol Biol 215: 403-410; see eg http://www.ncbi.nlm.nih.gov/BLAST/) with standard parameters. The determination of homology depends on the length of the compared sequences. In the context of the present invention a homology between two nucleic acid sequences, whose length is at least 100 nucleotides, is understood if at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 97%, at least 98%, or at least 99% of nucleotides are identical and/or similar ("identities" or "positives" according to BLAST), and preferably are identical. In the case of a sequence length of 50-99 nucleotides a homology between sequences is understood if there is identity or similarity of at least 80%, preferably at least 85%, 86%, 87%, 88% or 89%, with a sequence length of 15-49 nucleotides with an identity or similarity of at least 90%, preferably at least 95%, 96%, 97%, 98% or 99%. In the case of proteins a homology is assumed, if using the computer program BLAST with standard parameters and the BLOSUM62 substitution matrix (Henikoff, S., and Henikoff, J. Amino acid substitution matrices from protein blocks Proc. Natl. Acad. Sci. USA 89:. 10915-10919, 1992) an identity ("identities") and/or similarity ("positive"), preferably identity, at least 25%, at least 26%, at least 27%, at least 28%, at least 29% at least 30%, preferably at least 35%, at least 40%, at least 45%, at least 50%, at least 55%, at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 97%, at least 98%, or at least 99% is shown, preferably the entire length of the protein/peptide, which is compared with another protein, e.g. the length of 260 amino acids in the case of SEQ ID NO: 5 or the length of 245 amino acids in the case of SEQ ID NO: 6, is considered in determination. The person skilled in the art is able with his expert knowledge to use readily available BLAST programs (e.g. BLASTn, BLASTp, BLASTx, tBLASTn or tBLASTx) to determine the homology in question. In addition, there are other programs that the expert knows, and which he can use in the case in determining the homology of two or more comparative sequences or partial sequences. Such programs include those that can be found, for example on the website of the European Bioinformatics Institute (EMBL) (see, e.g. www.ebi.ac.uk/Tools/similarity.html).

[0032] The term "hybridizing" or "hybridization" means a process in which a single-stranded nucleic acid molecule attaches itself to a complementary nucleic acid strand, i.e. agrees with this base pairing. Standard procedures for hybridization are described, for example, in Sambrook et al. (Molecular Cloning. A Laboratory Manual, Cold Spring Harbor Laboratory Press, 3rd edition 2001). Preferably this will be understood to mean an at least 50%, more preferably at least 55%, 60%, 65%, 70%, 75%, 80% or 85%, more preferably 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or 99% of the bases of the nucleic acid strand form base pairs with the complementary nucleic acid strand. The possibility of such binding depends on the stringency of the hybridization conditions. The term "stringency" refers to hybridization conditions. High stringency is if base pairing is more difficult, low stringency, when a base-pairing is facilitated. The stringency of hybridization conditions depends for example on the salt concentration or ionic strength and temperature. Generally, the stringency can be increased by increasing the temperature and/or decreasing salinity. "Stringent hybridization conditions" are defined as conditions in which hybridization occurs predominantly only between homologous nucleic acid molecules. The term "hybridization conditions" refers not only to the actual binding of the nucleic acids at the prevailing conditions, but also in the subsequent washing steps prevailing conditions. Stringent hybridization conditions are, for example, conditions under which predominantly only those nucleic acid molecules having at least 70%, preferably at least 75%, at least 80%, at least 85%, at least 90% or at least 95% sequence identity hybridize. Less stringent hybridization conditions include: hybridization in 4.times.SSC at 37.degree. C., followed by repeated washing in 1.times.SSC at room temperature. Stringent hybridization conditions include: hybridization in 4.times.SSC at 65.degree. C., followed by repeated washing in 0.1.times.SSC at 65.degree. C. for a total of about 1 hour.

[0033] The term "complementary" refers to the ability of purine and pyrimidine nucleotides to form base pairs with each other via bridging hydrogen bonds. Complementary base pairs are, for example, guanine and cytosine, adenine and thymine and adenine and uracil. A complementary nucleic acid strand is accordingly a nucleic acid strand that can, by pairing with complementary bases of another nucleic acid strand, form a double strand.

[0034] As used herein, the term "homozygous" means a genetic condition existing when two alleles reside at a specific locus, but are positioned individually on corresponding pairs of homologous chromosomes in the cell and the two alleles are identical or at least identical for the one or more mutations.

[0035] A "fragment" or a "partial sequence" of a nucleic acid is here understood to be a contiguous section of the nucleic acid, i.e. a sequence segment of consecutive nucleotides of the nucleic acid.

[0036] Fragments can e.g. be used advantageously in a miRNA or RNAi approach, wherein the sequence can be used, for example, in anti-sense ("antisense") direction. "Anti-sense direction" or "antisense orientation" of a nucleic acid sequence, e.g. a DNA sequence, means here, for example, that a transcription of the DNA sequence results in an mRNA whose nucleotide sequence is complementary to a natural (endogenous) mRNA, so that its translation is hindered or prevented by the attachment of the complementary RNA. An "antisense RNA" or "antisense RNA" is understood to mean one of a particular mRNA or other RNAs complementary to specific RNA. "Anti-sense direction" or "antisense orientation" of an mRNA sequence, therefore, means that the mRNA has a sequence that is complementary to an mRNA sequence, so that its translation may be hindered or prevented by attachment. Partial sequences, which may be advantageously used in the context of the present invention, for example, in antisense orientation, are for example nucleic acids having a sequence shown in SEQ ID NO: 10, 11, 12 or 13 which is a segment of the nucleic acid according to SEQ ID NO: 1 or 2, and/or shown in SEQ ID NO: 14, 15, 16 or 17 which is a segment of the nucleic acid according to SEQ ID NO 3 or 4. However, any other nucleic acids with sequences or partial sequences of SEQ ID NOs: 1 or 2 and/or of SEQ ID NO: 3 or 4 can be used, for example, in the anti-sense direction. In addition, two or more partial sequences may be fused and advantageously used in the context of the present invention, for example, in antisense orientation. Examples of fused partial sequences are nucleic acids having a sequence shown in SEQ ID NO: 19, 20, 21 or 22, each of these sequences contains a segment of the nucleic acid according to SEQ ID NO: 1 or 2 as well as a segment of the nucleic acid according to SEQ ID NO: 3 or 4. In another embodiment two or more partial sequences may be fused and advantageously used in the context of the present invention, for example, in antisense orientation, whereby at least two fused partial sequences are derived from the same nucleic acid according to SEQ ID NO: 1, 2, 3 or 4. The fused partial sequences can be linked by a spacer sequence comprising at least 1, at least 2, at least 3, at least 4, at least 5, at least 6, at least 7, at least 8, at least 9, at least 10, at least 12, at least 14, at least 16, at least 18, at least 20, at least 25 or at least 30 nucleotides.

[0037] The partial sequence preferably comprises a nucleic acid with at least 25, preferably at least 30, 35, 40, 45, 50, 60, 70, 80, 90, or at least 100 consecutive nucleotides, more preferably at least 150, 184, 200, 212, 250, 300, 350, 400 or 450 consecutive nucleotides. A part of a protein (see, e.g., letter f) or F)) above) preferably comprises at least 5, preferably at least 10, 15, 20, 25, 30, 40 or 50, more preferably at least 60, 61, 70, 80, 90, or at least 100 consecutive amino acids of SEQ ID NO: 5 or 6. The sequence segment of SEQ ID NO: 5 or 6 (see, e.g., letter f) or F)) above) preferably comprises at least 50, 60, 61, 70, 80, 87 or 90, more preferably at least 100, 105, 120, 150, 200 or 250 consecutive amino acids of SEQ ID NO: 5 or 6. The necessary or useful length of the partial sequence of the nucleic acid or protein or the sequence segment can be selected by the person of ordinary skill in the art with the aid of his general technical skills and, where appropriate, by carrying out routine tests of the approach and the intended effect, without this requiring an inventive step.

[0038] The nucleic acid is preferably at least 85%, preferably at least 90%, 95%, 96%, 97%, 98% or 99%, more preferably at least 99.5%, 99.6%, 99.7%, 99.8% or 99.9% identical to a sequence or partial sequence of one of SEQ ID NO: 1-4.

[0039] The nucleic acid according to the invention may include one of the sequences of SEQ ID NO: 10-17 or 19-22, preferably in the antisense orientation.

[0040] In a preferred embodiment the inventive nucleic acid for the inhibition of bolting and flowering of a Beta vulgaris plant as described above comprises a further nucleic acid comprising a nucleotide sequence which (i) exhibits a sequence or partial sequence of SEQ ID NO: 7 or 8, or (ii) is complementary to a sequence or partial sequence of (i), or (iii) exhibits in the antisense direction a sequence or partial sequence of (i) or (ii), or (iv) is a homolog to a sequence or partial sequence of (i), or (v) at least 80% or 85%, preferably at least 90%, 95%, 96%, 97%, 98% or 99%, or more preferably at least 99.5%, 99, 6%, 99.7%, 99.8% or 99.9% identical to a sequence or partial sequence of (i), or (vi) encodes a protein or a part of the protein with the amino acid sequence of SEQ ID NO: 9, or (vii) encodes a protein with an amino acid sequence of Beta vulgaris which is a homolog to the sequence of F), or (viii) hybridizes under stringent conditions with a sequence or partial sequence of (i), (ii) or (iii).

[0041] In a further aspect, the present invention concerns the use of one or more of the inventive nucleic acids for the inhibition of bolting and flowering of a Beta vulgaris plant. As already indicated above, the nucleic acid can be introduced into a Beta vulgaris plant, for example, in antisense orientation or in form of a hairpin construct, thereby causing an inhibition of the genes responsible for bolting and flowering. Methods which are suitable for introducing a nucleic acid in a Beta vulgaris cell are known to the skilled person, and include for example the Agrobacterium-mediated transformation (Lindsey, K., and P. Gallois. "Transformation of sugarbeet (Beta vulgaris) by Agrobacterium tumefaciens." Journal of experimental botany 41.5 (1990): 529-536). The introduction of a nucleic acid in antisense orientation into a plant is only one of the known processes for the inhibition or suppression of gene activity (gene silencing).

[0042] The inventive nucleic acids can also be used advantageously in the context of other procedures or mechanisms that can cause an inhibition or suppression of bolting/flowering, e.g. suppression by co-expression, or as template for the generation of guide RNA (gRNA) in a CRISPR/Cas system.

[0043] Furthermore, the inventive nucleic acid may also be used as a probe to identify other factors, genes or gene products, which can be used to inhibit or suppress flowering and bolting of Beta vulgaris plants, or may also be used as molecular marker to detect or to identify in a mutagenized Beta vulgaris plant or a part thereof one or more mutations in an endogenous DNA sequence or a regulatory sequence of the endogenous DNA sequence, wherein the endogenous DNA sequence has a nucleic acid sequence identical to a sequence which (a) exhibits a sequence or partial sequence of SEQ ID NO: 1, or (b) is complementary to a sequence or partial sequence of (a), or (c) exhibits in the antisense direction a sequence or partial sequence of (a) or (b), or (d) is a homolog to a sequence or partial sequence of (a), or (e) at least 80% or 85%, preferably at least 90%, 95%, 96%, 97%, 98% or 99%, or more preferably at least 99.5%, 99, 6%, 99.7%, 99.8% or 99.9% identical to a sequence or partial sequence of a), or (f) encodes a protein or a part of the protein with the amino acid sequence of SEQ ID NO: 5, or (g) encodes a protein with an amino acid sequence of Beta vulgaris which is a homolog to the sequence of (f), or (h) hybridizes under stringent conditions with a sequence or partial sequence of a), b) or c) and/or (A) exhibits a sequence or partial sequence of SEQ ID NO: 3, or (B) is complementary to a sequence or partial sequence of (A), or (C) exhibits in the antisense direction a sequence or partial sequence of (A) or (B), or (D) is a homolog to a sequence or partial sequence of (A), or (E) at least 80% or 85%, preferably at least 90%, 95%, 96%, 97%, 98% or 99%, or more preferably at least 99.5%, 99, 6%, 99.7%, 99.8% or 99.9% identical to a sequence or partial sequence of (A), or (F) encodes a protein or a part of the protein with the amino acid sequence of SEQ ID NO: 6, or (G) encodes a protein with an amino acid sequence of Beta vulgaris which is a homolog to the sequence of (F), or (H) hybridizes under stringent conditions with a sequence or partial sequence of (A), (B) or (C). Preferably, the one or more mutations cause a reduced transcriptional or expressional rate or a reduced transcriptional or expressional level of the endogenous DNA sequence in the mutagenized plant compared to a non-mutagenized wildtype plant, or the mutation causes a reduction of the activity or stability of the protein or polypeptide encoded by the endogenous DNA sequence compared to a non-mutagenized wildtype plant. More preferably, at least one of the one or more mutations is selected from the group consisting of mutations listed in Table 1.

[0044] In a particular embodiment of the present invention the use of the one or more nucleic acids as described above includes in addition to the one or more nucleic acids a further nucleic acid comprising a nucleotide sequence which (i) exhibits a sequence or partial sequence of SEQ ID NO: 7 or 8, or (ii) is complementary to a sequence or partial sequence of (i), or (iii) exhibits in the antisense direction a sequence or partial sequence of (i) or (ii), or (iv) is a homolog to a sequence or partial sequence of (i), or (v) at least 80% or 85%, preferably at least 90%, 95%, 96%, 97%, 98% or 99%, or more preferably at least 99.5%, 99, 6%, 99.7%, 99.8% or 99.9% identical to a sequence or partial sequence of (i), or (vi) encodes a protein or a part of the protein with the amino acid sequence of SEQ ID NO: 9, or (vii) encodes a protein with an amino acid sequence of Beta vulgaris which is a homolog to the sequence of (vi), or (viii) hybridizes under stringent conditions with a sequence or partial sequence of (i), (ii) or (iii). These nucleic acids may be used for the inhibition of bolting and flowering of Beta vulgaris plant.

[0045] In a further aspect the present invention concerns a protein with an amino acid sequence of SEQ ID NO: 5 or 6, or a protein having an amino acid sequence that contains a sequence segment of SEQ ID NO: 5 or 6, that comprises preferably at least 50, 60, 61, 70, 80 or 90, at least 100, 120, 150, 200 or 250 consecutive amino acids of SEQ ID NO: 5 or 6, or a thereto homologous protein from Beta vulgaris. The protein or any part thereof, or the corresponding amino acid sequences may/could for example be used as a probe at the amino acid level to identify other factors, genes or gene products which can be used to inhibit and/or suppress the flowering and bolting of a Beta vulgaris plants.

[0046] In another aspect the present invention relates to a method for producing a transgenic Beta vulgaris plant comprising the steps of (a) transforming a Beta vulgaris cell with one or more inventive nucleic acids and (b) regenerating a Beta vulgaris plant from the transformed Beta vulgaris cell. The transformation of Beta vulgaris cell can occur, for example, using known vectors, e.g. a Ti-plasmid, and is known to the skilled person (Lindsey and Gallois, 1990). The inventive nucleic acids may be found advantageous under the control of a suitable promoter in such a vector. In a particular embodiment of the present invention the method for producing a transgenic Beta vulgaris plant, where the bolting and flowering is inhibited after vernalization, comprises the following steps of: (I) Transforming a Beta vulgaris cell with a first nucleic acid as transgene and a second nucleic acid as transgene, wherein the Beta vulgaris cell is transformed with one construct comprising the first and the second nucleic acid or with a construct comprising the first nucleic acid and another construct comprising the second nucleic acid; and (II) regenerating a Beta vulgaris plant from the transformed Beta vulgaris cell. Thereby, the first nucleic acid as transgene comprises a nucleotide sequence which a) exhibits a sequence or partial sequence of SEQ ID NO: 1 or 2, orb) is complementary to a sequence or partial sequence of a), or c) exhibits in the antisense direction a sequence or partial sequence of a) or b), or d) is a homolog to a sequence or partial sequence of a), or e) at least 80% or 85%, preferably at least 90%, 95%, 96%, 97%, 98% or 99%, or more preferably at least 99.5%, 99, 6%, 99.7%, 99.8% or 99.9% identical to a sequence or partial sequence of a), or f) encodes a protein or a part of the protein with the amino acid sequence of SEQ ID NO: 5, or g) encodes a protein with an amino acid sequence of Beta vulgaris which is a homolog to the sequence off), or h) hybridizes under stringent conditions with a sequence or partial sequence of a), b) or c); and the second nucleic acid as transgene comprises a nucleotide sequence which A) exhibits a sequence or partial sequence of SEQ ID NO: 3 or 4, or B) is complementary to a sequence or partial sequence of A), or C) exhibits in the antisense direction a sequence or partial sequence of A) or B), or D) is a homolog to a sequence or partial sequence of A), or E) at least 80% or 85%, preferably at least 90%, 95%, 96%, 97%, 98% or 99%, or more preferably at least 99.5%, 99, 6%, 99.7%, 99.8% or 99.9% identical to a sequence or partial sequence of A), or F) encodes a protein or a part of the protein with the amino acid sequence of SEQ ID NO: 6, or G) encodes a protein with an amino acid sequence of Beta vulgaris which is a homolog to the sequence of F), or H) hybridizes under stringent conditions with a sequence or partial sequence of A), B) or C). In an alternative particular embodiment of the present invention the method for producing a transgenic Beta vulgaris plant, where the bolting and flowering is inhibited after vernalization, comprises the following steps of: (I) Transforming a first Beta vulgaris cell with a first nucleic acid as transgene as defined above; (II) transforming a second Beta vulgaris cell with a second nucleic acid as transgene as defined above; and (III) regenerating a first Beta vulgaris plant from the transformed first Beta vulgaris cell and a second Beta vulgaris plant from the transformed second Beta vulgaris cell; and (IV) crossing the first Beta vulgaris plant with the second Beta vulgaris plant and selecting a progeny comprising the first nucleic acid and the second nucleic acid as transgenes.

[0047] The invention also relates to a vector or a mobile genetic element that includes one or more inventive nucleic acids. Vectors and mobile genetic elements are known in the art and include, for example, plasmids such as the Ti-plasmid. The vector or mobile genetic element can advantageously contain control/regulatory elements, e.g. a promoter, enhancer, intronic sequence, or terminator.

[0048] Furthermore, the invention relates to a transgenic Beta vulgaris plant including one or more inventive nucleic acids as transgene, preferably under the control of a suitable promoter, and which is inhibited in bolting and flowering, as well as seeds and/or parts of a Beta vulgaris plant transformed with one or more inventive nucleic acids.

[0049] In a further aspect the present invention relates to a method for producing a Beta vulgaris plant, preferably a non-transgenic Beta vulgaris plant, where the bolting and flowering is inhibited after vernalization. In one embodiment the method for producing a Beta vulgaris plant comprises the following steps: (I) Mutagenizing one or more parts of a Beta vulgaris plant and subsequently regenerating Beta vulgaris plants from the one or more parts, or mutagenizing one or more Beta vulgaris plants, (II) identifying a plant of (I) which exhibits one or more mutations in a first endogenous DNA sequence and/or in a regulatory sequence thereof and exhibits one or more mutations in a second endogenous DNA sequence and/or in a regulatory sequence thereof, and optionally (III) generating a Beta vulgaris plant in which the one or more mutations in the first endogenous DNA sequence and the one or more mutations in the second endogenous DNA sequence are homozygous. The first endogenous DNA sequence has a nucleic acid sequence identical to a sequence which (a) exhibits a sequence or partial sequence of SEQ ID NO: 1, or (b) is complementary to a sequence or partial sequence of (a), or (c) exhibits in the antisense direction a sequence or partial sequence of (a) or (b), or (d) is a homolog to a sequence or partial sequence of (a), or (e) at least 80% or 85%, preferably at least 90%, 95%, 96%, 97%, 98% or 99%, or more preferably at least 99.5%, 99, 6%, 99.7%, 99.8% or 99.9% identical to a sequence or partial sequence of a), or (f) encodes a protein or a part of the protein with the amino acid sequence of SEQ ID NO: 5, or (g) encodes a protein with an amino acid sequence of Beta vulgaris which is a homolog to the sequence of (f), or (h) hybridizes under stringent conditions with a sequence or partial sequence of a), b) or c) and the second endogenous DNA sequence has a nucleic acid sequence identical to a sequence which (A) exhibits a sequence or partial sequence of SEQ ID NO: 3, or (B) is complementary to a sequence or partial sequence of (A), or (C) exhibits in the antisense direction a sequence or partial sequence of (A) or (B), or (D) is a homolog to a sequence or partial sequence of (A), or (E) at least 80% or 85%, preferably at least 90%, 95%, 96%, 97%, 98% or 99%, or more preferably at least 99.5%, 99.6%, 99.7%, 99.8% or 99.9% identical to a sequence or partial sequence of (A), or (F) encodes a protein or a part of the protein with the amino acid sequence of SEQ ID NO: 6, or (G) encodes a protein with an amino acid sequence of Beta vulgaris which is a homolog to the sequence of (F), or (H) hybridizes under stringent conditions with a sequence or partial sequence of (A), (B) or (C). In another embodiment the method for producing a Beta vulgaris plant comprises the following steps: (I) Mutagenizing one or more parts of a Beta vulgaris plant and subsequently regenerating one or more Beta vulgaris plants from the one or more parts, or mutagenizing one or more Beta vulgaris plants; (II) identifying a first plant of (I) which exhibits one or more mutations in a first endogenous DNA sequence as defined above and/or in a regulatory sequence thereof and a second plant of (I) which exhibits one or more mutations in a second endogenous DNA sequence as defined above and/or in a regulatory sequence thereof; (III) crossing the first plant with the second plant and selecting a progeny comprising the one or more mutations in the first endogenous DNA sequence and/or in a regulatory sequence thereof and the one or more mutations in the second endogenous DNA sequence and/or in a regulatory sequence thereof; and optionally (IV) generating from the progeny of (III) a Beta vulgaris plant in which the one or more mutations in the first endogenous DNA sequence and the one or more mutations in the second endogenous DNA sequence are homozygous.

[0050] In a preferred embodiment the step of mutagenizing comprises the steps of: i) Subjecting pollen or seeds of a Beta vulgaris plant to a sufficient amount of the mutagen ethylmethane sulfonate (EMS) or other mutagenic chemicals or mutagenic radiation to obtain M1 plants, ii) optionally allowing sufficient production of M2 plants, and iii) isolating and analysing genomic DNA of M1 and/or M2 plants.

[0051] Preferably, the one or more mutations cause a reduced transcriptional or expressional rate or a reduced transcriptional or expressional level of the endogenous DNA sequence in the mutagenized plant compared to a non-mutagenized wildtype plant, or the mutation causes a reduction of the activity or stability of the protein or polypeptide encoded by the endogenous DNA sequence compared to a non-mutagenized wildtype plant. More preferably, the one or more mutations result in a loss of function, i.e. the expression/transcription of the mutated DNA does not lead to the synthesis of a functional protein (e.g. functional AP1 protein or FUL protein, respectively).

[0052] The one or more mutations may cause an alteration of the amino acid sequence of AP1 or FUL, in particular the one or more mutations can be a point mutation resulting in at least one amino acid exchange, the exchange of an amino acid coding codon to a codon carrying the stop signal of translation (stop codon), or the change of the start signal of translation (start codon). The techniques of introducing such mutations via mutagenizing are well-known to the person skilled in the art. In a preferred embodiment, wherein the one or more mutations are effected in the endogenous AP1 gene or FUL gene, the obtained Beta vulgaris plant is non-transgenic. Preferably, the mutation is effected via non-transgenic mutagenesis, transposon mutagenesis, in particular chemical mutagenesis, preferably via EMS (ethylmethane sulfonate)-induced TILLING or targeted genome editing (e.g. CRISPR/Cas, TALEN, Zinc Finger nucleases, etc.). Exemplary, Table 1a and 1b show possible point mutations within the genomic DNA and cDNA of AP1 and FUL resulting in a nucleotide exchange from cytosine (c) to thymine (t) and thereby generating a stop codon. Such mutations can reduce the activity or stability of the corresponding protein or polypeptide encoded by the endogenous DNA sequence compared to a non-mutagenized wildtype plant, or can result in a loss of function of the corresponding protein.

[0053] Additionally, the one or more mutations may cause an alteration of the amino acid sequence of the AP1 protein or FUL protein by an insertion or deletion of one or more amino acids, e.g. through a shift of the open reading frame. The insertion can be introduced for instances by transposon mutagenesis and deletion can be created for instances by genomic engineering. Insertion and deletion can occur in any nucleotide sequence encoding one of the above described proteins, in a nucleotide sequence of an intron or in a nucleotide sequence of the 5' untranslated region (UTR) or 3' UTR of the AP1 gene or FUL gene. The insertion can have a length of at least 1 nucleotide, at least 2 nucleotides, at least 3 nucleotides, at least 4 nucleotides, at least 5 nucleotides, at least 6 nucleotides, at least 7 nucleotides, at least 8 nucleotides, at least 9 nucleotides, at least 10 nucleotides, at least 12 nucleotides, at least 14 nucleotides, at least 16 nucleotides, at least 18 nucleotides, at least 20 nucleotides, at least 25 nucleotides, at least 30 nucleotides, at least 40 nucleotides, at least 50 nucleotides, at least 75 nucleotides, at least 100 nucleotides, at least 200 nucleotides, at least 300 nucleotides, or at least 500 nucleotides. If such insertion or deletion occurs for instances in a regulatory element (e.g. promotor), that may reduce the transcriptional or expressional rate or a reduced transcriptional or expressional level of the corresponding endogenous DNA sequence in the Beta vulgaris plant cell.

[0054] As used herein, the term "reduced expressional rate" or "reduced expressional level" means a reduction of the expressional rate or of the expressional level of one or more nucleic acid sequences by more than 25% or 30%, preferably by more than 40%, 45%, 50%, 55%, 60%, or 65%, more preferably by more than 70%, 75%, 80%, 85%, 90%, 92%, 94%, 96% or 98% compared to the given reference. It may be that the reduction of the expressional rate or of the expressional level is 100%, e.g. in case of knock-out mutants or loss of function mutants. Preferably the reduced expressional rate or expressional level results in an amended phenotype where the bolting and flowering is inhibited after vernalization. The term "reduced transcriptional rate" or "reduced transcriptional level" means a reduction of the transcriptional rate or of the transcriptional level of one or more nucleic acid sequences by more than 25% or 30%, preferably by more than 40%, 45%, 50%, 55%, 60%, or 65%, more preferably by more than 70%, 75%, 80%, 85%, 90%, 92%, 94%, 96% or 98% compared to the given reference. It may be that the reduction of the transcriptional rate or of the transcriptional level is 100%, e.g. in case of knock-out mutants or loss of function mutants. Preferably the reduced transcriptional rate or transcriptional level results in an amended phenotype where the bolting and flowering is inhibited after vernalization.

TABLE-US-00001 TABLE 1a List of positions in genomic DNA and cDNA of BvAP1 where a point mutation causes a nucleotide exchange from cytosine (c) to thymine (t) generating a stop codon. Gene Position in genomic Position in cDNA name DNA (SEQ ID NO: 1) (SEQ ID NO: 2) BvAP1 52 52 BvAP1 151 151 BvAP1 6999 262 BvAP1 7640 316 BvAP1 8573 343 BvAP1 8606 376 BvAP1 8618 388 BvAP1 8621 391 BvAP1 8648 418 BvAP1 11796 433 BvAP1 11826 463 BvAP1 21152 484 BvAP1 21313 541 BvAP1 21319 547 BvAP1 21322 550 BvAP1 21328 556 BvAP1 21334 562 BvAP1 21340 568 BvAP1 21346 574 BvAP1 21352 580 BvAP1 21358 586 BvAP1 21361 589 BvAP1 21364 592 BvAP1 21382 610 BvAP1 21391 619 BvAP1 21576 688 BvAP1 21582 694

TABLE-US-00002 TABLE 1b List of positions in genomic DNA and cDNA of BvFUL where a point mutation causes a nucleotide exchange from cytosine (c) to thymin (t) generating a stop codon. Gene Position in genomic Position in cDNA name DNA (SEQ ID NO: 3) (SEQ ID NO: 4) BvFUL 19 19 BvFUL 52 52 BvFUL 19373 235 BvFUL 19552 316 BvFUL 19561 325 BvFUL 19689 376 BvFUL 19704 391 BvFUL) 28161 433 BvFUL 28185 457 BvFUL 28191 463 BvFUL 28194 466 BvFUL 28447 541 BvFUL 28450 544 BvFUL 28465 559 BvFUL 28468 562 BvFUL 28507 601 BvFUL 29166 664 BvFUL 29196 694 BvFUL 29217 715 BvFUL 29226 724

[0055] Thus, the present invention relates to a Beta vulgaris plant, preferably a non-transgenic Beta vulgaris plant, where the bolting and flowering is inhibited after vernalization, wherein the plant exhibits one or more mutations in a first endogenous DNA sequence and/or in a regulatory sequence thereof and exhibits one or more mutations in a second endogenous DNA sequence and/or in a regulatory sequence thereof The first endogenous DNA sequence has a nucleic acid sequence identical to a sequence which (a) exhibits a sequence or partial sequence of SEQ ID NO: 1, or (b) is complementary to a sequence or partial sequence of (a), or (c) exhibits in the antisense direction a sequence or partial sequence of (a) or (b), or (d) is a homolog to a sequence or partial sequence of (a), or (e) at least 80% or 85%, preferably at least 90%, 95%, 96%, 97%, 98% or 99%, or more preferably at least 99.5%, 99.6%, 99.7%, 99.8% or 99.9% identical to a sequence or partial sequence of a), or (f) encodes a protein or a part of the protein with the amino acid sequence of SEQ ID NO: 5, or (g) encodes a protein with an amino acid sequence of Beta vulgaris which is a homolog to the sequence of (f), or (h) hybridizes under stringent conditions with a sequence or partial sequence of a), b) or c) and the second endogenous DNA sequence has a nucleic acid sequence identical to a sequence which (A) exhibits a sequence or partial sequence of SEQ ID NO: 3, or (B) is complementary to a sequence or partial sequence of (A), or (C) exhibits in the antisense direction a sequence or partial sequence of (A) or (B), or (D) is a homolog to a sequence or partial sequence of (A), or (E) at least 80% or 85%, preferably at least 90%, 95%, 96%, 97%, 98% or 99%, or more preferably at least 99.5%, 99, 6%, 99.7%, 99.8% or 99.9% identical to a sequence or partial sequence of (A), or (F) encodes a protein or a part of the protein with the amino acid sequence of SEQ ID NO: 6, or (G) encodes a protein with an amino acid sequence of Beta vulgaris which is a homolog to the sequence of (F), or (H) hybridizes under stringent conditions with a sequence or partial sequence of (A), (B) or (C). Preferably, the one or more mutations in the first and/or the second endogenous DNA sequence are homozygous.

[0056] Preferably, the one or more mutations cause a reduced transcriptional or expressional rate or a reduced transcriptional or expressional level of the endogenous DNA sequence in the mutagenized plant compared to a non-mutagenized wildtype plant, or the mutation causes a reduction of the activity or stability of the protein or polypeptide encoded by the endogenous DNA sequence compared to a non-mutagenized wildtype plant. More preferably, the one or more mutations result in a loss of function, i.e. the expression/transcription of the mutated DNA does not lead to the synthesis of a functional protein (e.g. functional AP1 protein or FUL protein, respectively).

[0057] In a preferred embodiment of the Beta vulgaris plant or a part thereof of the present invention is a Beta vulgaris plant or a part thereof as described above wherein the one or more mutations cause an alteration of the amino acid sequence of AP1 or FUL, in particular the one or more mutations is a point mutation resulting in at least one amino acid exchange, the exchange of an amino acid coding codon to a codon carrying the stop signal of translation (stop codon), or the change of the start signal of translation (start codon). Preferably the point mutation in the AP1 gene (i.e. first endogenous DNA sequence) is selected from the group consisting of mutations listed in Table 1a or indicated by SEQ ID NO: 33 or SEQ ID NO: 37 and/or the point mutation in the FUL gene (i.e. second endogenous DNA sequence) is selected from the group consisting of mutations listed in Table 1b or indicated by SEQ ID NO: 34 or SEQ ID NO: 38. Corresponding positions in allelic variants of AP1 and FUL are also included. More preferably, the point mutation in the AP1 gene (i.e. first endogenous DNA sequence) is a nucleotide exchange from cytosine (c) to thymine (t) at position 262 of SEQ ID NO: 2 or at position 6999 of SEQ ID NO: 1 or corresponding position in allelic variants of AP1, and/or the point mutation in the FUL gene (i.e. second endogenous DNA sequence) is a nucleotide exchange from cytosine (c) to thymine (t) at position 316 of SEQ ID NO: 4 or at position 19552 of SEQ ID NO: 3 or corresponding position in allelic variants of FUL. Consequently, the mutated AP1 gene (i.e. first endogenous DNA sequence) can have the sequence of SEQ ID NO: 39 leading to a cDNA according to SEQ ID NO: 35, and/or the mutated FUL gene (i.e. second endogenous DNA sequence) can have the sequence of SEQ ID NO: 40 leading to a cDNA according to SEQ ID NO: 36.

[0058] In an additional aspect of the invention the above described methods for producing a transgenic or non-transgenic Beta vulgaris plant can be combined. That means for instances that only one of the first and second endogenous DNA sequences have been mutated by introducing one or more mutations and the expression of the other, non-mutated DNA sequence is suppressed or silenced using the transformation approach as described above.

[0059] A further aspect of the invention is a Beta vulgaris plant or a part thereof produced or producible by any of the methods for producing a Beta vulgaris plant as described above.

[0060] The invention is described below with reference to exemplary embodiments and the accompanying figures purely for illustrative purposes.

BRIEF DESCRIPTION OF THE FIGURES

[0061] FIG. 1: Schematic structure of cloning vector pAB70S-1 35S Ataap6 RNAi

[0062] FIG. 2: Schematic structure of cloning vector pAB70S-1 35S Ataap6 FUL AP1 including partial sequences of AP1 and FUL

[0063] FIG. 3: Schematic structure of binary Ti-plasmid pZFN d35S RNAi FUL-AP1 including partial sequences of AP1 (212 bp) and FUL (184 bp) used in Agrobacterium-mediated transformation

[0064] FIG. 4: Schematic representation of cloning vector pAB70s-1 d35S Ataap6 RNAi viI1-AP1-FUL LF including partial sequences of AP1, FUL and VIL1

[0065] FIG. 5: Schematic representation of the binary Ti-plasmid pZFN d35S RNAi vil1-FUL-AP1 including partial sequences of AP1, FUL and VIL1 used in Agrobacterium-mediated transformation

[0066] FIG. 6: Alignment of the amino acid sequences of the protein AP1 from Beta vulgaris (SEQ ID NO: 5) and from Arabidopsis thaliana (SEQ ID NO: 41) based on the EMBOSS Needle algorithm; BvAP1 =Beta vulgaris AP1, AtAP1=Arabidopsis thaliana AP1

[0067] FIG. 7: Alignment of the amino acid sequence of the protein FUL from Beta vulgaris (SEQ ID NO: 6) and from Arabidopsis thaliana (SEQ ID NO: 42) based on the EMBOSS Needle algorithm; BvFUL=Beta vulgaris FUL, AtFUL=Arabidopsis thaliana FUL

EXAMPLES

[0068] 1. Inhibition of bolting and flowering by RNAi constructs targeted to AP1 and FUL

[0069] Identification/isolation and characterization/annotation of complete cDNAs of sugar beet for inhibition of bolting and flowering:

[0070] By analysis within a specially created proprietary sugar beet EST database, the 780 base pairs (bp) long cDNA (SEQ ID NO: 2) of BvAP1 as well as the 735 bp long cDNA (SEQ ID NO: 4) of BvFUL have been identified. In addition, corresponding genomic DNA sequences could be identified. An alignment of genomic DNA with cDNA shows the structures of the entire DNAs. AP1 consists of 8 exons and 7 introns, FUL of 8 exons and 7 introns.

[0071] A comparison of the resulting full-length DNA and the translated protein sequence shows only low sequence similarity with Arabidopsis homologs AtAP1 and AtFUL. At protein level the identity over the entire sequence length to AtAP1 is at 65.6% (see also FIG. 6) and to AtFUL is at 57.3% (see also FIG. 7), at cDNA level the identity AtAP1 is 71% and to AtFUL is 72% (see Table 2).

TABLE-US-00003 TABLE 2 Sequence comparison of BvAP1 and BvFUL with Arabidopsis thaliana (At)-AP1 and -FUL- candidates based on the protein sequence and cDNA. Results given as sequence identity based on the EMBOSS Needle algorithm (www.ebi.ac.uk). AtAP1 protein AtFUL protein AtAP1 AtFUL (SEQ ID NO: 41) (SEQ ID NO: 42) cDNA cDNA BvAP1 protein 65.6% (SEQ ID NO: 5) BvFUL protein 57.3% (SEQ ID NO: 6) BvAP1 cDNA 71% (SEQ ID NO: 2) BvFUL cDNA 72% (SEQ ID NO: 4)

[0072] Production of RNAi constructs targeted to AP1 and FUL and inhibition of bolting and flowering in sugar beet:

[0073] For the production of RNAi constructs the sequences of SEQ ID NO: 19 to 22 were synthesized. Sequence of SEQ ID NO: 19 includes a partial sequence of AP1 according to SEQ ID NO 10 with a length of 184 bp and a partial sequence of FUL according to SEQ ID NO 14 with a length of 212 bp. Sequence of SEQ ID NO: 20 includes a partial sequence of AP1 according to SEQ ID NO 11 with a length of 150 bp and a partial sequence of FUL according to SEQ ID NO 15 with a length of 150 bp. Sequence of SEQ ID NO: 21 includes a partial sequence of AP1 according to SEQ ID NO 12 with a length of 100 bp and a partial sequence of FUL according to SEQ ID NO 16 with a length of 100 bp. Sequence of SEQ ID NO: 22 includes a partial sequence of AP1 according to SEQ ID NO 13 with a length of 50 bp and a partial sequence of FUL according to SEQ ID NO 17 with a length of 50 bp.

[0074] For the further processing the sequences have been amplified by PCR using PCR Primers with SalI/SmaI restriction sites like primers according to SEQ ID NO: 25 (forward) and 26 (reverse) for the amplification of SEQ ID NO 19. The PCR was performed using 10 ng of genomic sugar beet DNA, a primer concentration of 0.2 micron at an "annealing" temperature of 55.degree. C. in a Multicycler PTC-200 (MJ Research, Watertown, USA).

[0075] The PCR products were each integrated into the vector pAB70S-1 35S Ataap6 RNAi (FIG. 1). The vector is designed for the production of "intron-spliced" hairpin structures. The vector contains the d35S promoter for constitutive expression, the ATAAP6 intron from Arabidopsis thaliana and one polyA terminator (nos-T). The ATAAP6 intron is flanked by the interfaces or cleavage sites XhoI/Ecl136II on the 5' end or by the restriction cleavage sites SmaI/SalI at the 3'-end. This enables the integration of identical fragments in a "sense" and "antisense", if these fragments have the compatible restriction sites XhoI or SalI, or are stumped on the other end ("blunt end"). For this the original PCR products were reamplified with new PCR primers extended beyond these restriction sites. For further use the PCR fragments were cloned into the TA cloning vector pCR2.1 (TOPO TA Cloning Kit (Invitrogen, Carlsbad, USA)) and transformed in E. coli. A blue-white selection enabled the identification of recombinant plasmids (Sambrook et al. 201, in Molecular Cloning A Laboratory Manual, Cold Spring Harbor Laboratory Press, 3rd edition, New York). In the white colonies the expression of ss-galactosidase is suppressed by an insert, which results in white colonies, because the enzyme substrate added to the medium is no longer cleaved. After a subsequent sequencing with M13-fwd/rev-primers, the analysis and the alignment of the sequence data was performed using the program Vector NTI (Invitrogen, Carlsbad, USA).

[0076] The fragments Sal-SmaI and XhoI-SmaI-were each cut from the topovector by SalI/SmaI or XhoI/SmaI and then subsequently first ligated "in sense" with the SalI/SmaI or XhoI/Ecl136II cut pRTRNAi vector. Subsequently, the same fragments were religated for a second time in "antisense" in the compatible XhoI/Ecl136II or SalI/SmaI. The cloning resulted in, for example, pAB70S-1 35S Ataap6 FUL AP1 (FIG. 2).

[0077] Production of transformation constructs and sugar beet transformation:

[0078] For plant transformation the binary vector pZFN was used. The expression cassettes were cut using SfiI to transfer it into the binary vector pZFN to create pZFN d35S RNAi FUL-AP1-212-184 (FIG. 3), pZFN d35S RNAi FUL-AP1-150-150, pZFN d35S RNAi FUL-AP1-100-100, and pZFN d35S RNAi FUL-AP1-50-50. Each of the binary vectors was transformed in Agrobacterium tumefaciens strain GV3101 pMP90 by a direct DNA transformation method (An, G. (1987), Ti binary vectors for plant transformation and promoter analysis, Methods Enzymol. 153, 292-305). The selection of recombinant A. tumefaciens clones was performed using the antibiotic streptomycin (50 mg /1). The transformation of sugar beet and regeneration were carried out according to Lindsey et al. (1990) and Lindsey et al. (1991, "Regeneration and transformation of sugar beet by Agrobacterium tumefaciens, Plant Tissue Culture Manual B7: 1-13, Kluwer Academic Publishers).

[0079] The transgenicity of the plants was verified by PCR. The use of designed primers led to the amplification of a particular DNA fragment from the nptII gene. The PCR was performed using 10 ng genomic DNA, a primer concentration of 0.2 micron at an annealing temperature of 55.degree. C. in a Multicycler PTC-200 (MJ Research, Watertown, USA).

[0080] Verification of the flowering and bolting behavior of the transformants:

[0081] For each of the RNAi constructs five transgenic sugar beet lines were regenerated carrying the corresponding binary vector pZFN d35S RNAi FUL-AP1. The sugar beet plants were grown for several weeks in sterile culture media, propagated and then rooted together with non-transgenic isogenic controls. 7-9 plants per line and control were transferred to the greenhouse. The transgenic lines were grown in pots and then tested in different vernalization regimes to determine the bolting and flowering behavior. After an adjustment period, the plants were subjected to vernalization for three, four and six months at 8.degree. C. in a cooling chamber (winter simulation). Subsequently, the transformants, as well as identically treated non-transgenic control plants, were transferred back into the greenhouse (25.degree. C.). Shortly after transfer, already after 10 days, the control plants began to grow shoots. After 4 weeks the control plants began to bloom. In contrast, several transformants lines showed surprisingly no response to the shoot and flower induction by vernalization. Hereunder were lines of each used RNAi construct.

[0082] Thus, the resulting transformants behaved like not-vernalized sugar beets. They neither developed shoots nor blooms. None of the plants showed deviations from the normal phenotype. The plants were cultivated further; they continued to develop to normal beets with normal beet bodies.

[0083] These lines were again tested in a greenhouse supplied with soil for optimal root growth without temperature control in two winters (2013/2014; 2014/2015). None of the plants did bolt or flower.

[0084] Surprisingly, using the inventive approach, the vernalization or its effect, namely the bolting and flowering, were completely blocked in sugar beet.

[0085] 2. Inhibition of bolting and flowering by RNAi constructs targeted to AP1, FUL and VIL1

[0086] For this approach an RNAi construct comprising partial sequences of AP1 and FUL cDNA was extended by a third partial sequence of 399 bp based on cDNA of the BvVil1 gene from Beta vulgaris (WO 2011/032537). VIL1 was chosen due to its involvement in flower formation.

[0087] PCR product BvVil1 RNAi was amplified using a forward primer according to SEQ ID NO: 27 and a reverse primer according to SEQ ID NO: 28. BvVil1 cDNA was used as template. The amplification led to a VIL1 cDNA fragment according to SEQ ID NO: 29. Additionally, PCR product BvFUL-AP1 was synthesized and amplified using primers according to SEQ ID NO: 25 and 26. Vector pZFN as described above was used as template. The amplification led to a FUL-AP1 cDNA fragment according to SEQ ID NO: 30.

[0088] Both amplified DNAs were cloned into vector pAB70S-1 35S Ataap6 RNAi (FIG. 1). PCR product BvVil1 RNAi was cloned using Ecl136II and XhoI. PCR product BvFUL-AP1 was added using XhoI and SmaI. The resulting intermediate pAB70s-1 d35S Ataap6 RNAi vil1-AP1-FUL LF (FIG. 4) was used for another PCR step. The vector pAB70s-1 d35S Ataap6 RNAi vil1-AP1-FUL LF was used as template for forward primer according to SEQ ID NO: 31 and reverse primer according to SEQ ID NO: 32. The PCR product was an RNAi construct which then was cloned into the vector using SalI and SmaI. The resulting vector was cut using SfiI and cloned into vector pZFN resulting in vector pZFN d35S RNAi vil1-FUL-AP1 (FIG. 5).

[0089] Vector pZFN d35S RNAi vil1-FUL-AP1 was used to transform Agrobacterium tumefaciens Gv3101 pmp90 which subsequently was used to generate transgenic sugar beet lines. Transgenicity was confirmed by PCR. After regeneration nine plants of one line were rooted as described above. After vernalization in the greenhouse none of the plants did bolt or flower.

[0090] 3. Inhibition of bolting and flowering by knock-out mutants of BvAP1 and BvFUL

[0091] Mutagenization of sugar beet cells and identification of BvAP1 and BvFUL mutants:

[0092] A sugar beet mutant population has been created by treatment with different EMS concentrations for different durations of incubation. From treated cells M1 plants could be regenerated. Through selfing of the M1 plants several thousands of M2 plants were grown.

[0093] These M2 plants were screened for knock out mutations in the BvAP1 gene and the BvFUL gene. For that, DNA was been extracted from collected leaf samples and analysed by use of designed primers. Thereby, point mutations in the genes which introduce additional stop codons into the coding sequence of the genes could be identified. One plant showed a point mutation in the AP1 gene which is a nucleotide exchange from cytosine (c) to thymine (t) at position 262 of the cDNA (SEQ ID NO: 2) or at position 6999 of the genomic DNA (SEQ ID NO: 1). A second identified plant contained a point mutation in the FUL gene which is a nucleotide exchange from cytosine (c) to thymine (t) at position 316 of the cDNA (SEQ ID NO: 4) or at position 19552 of the genomic DNA (SEQ ID NO: 3).

[0094] Verification of the flowering and bolting behavior of single mutants:

[0095] Cells of the identified sugar beet mutants were cultured for several weeks in sterile culture media, propagated and then rooted together with non-mutated controls. 5 plants per mutant and control were transferred to the greenhouse. The mutant lines were grown in pots and then tested in different vernalization regimes to determine the bolting and flowering behavior. After an adjustment period, the plants were subjected to vernalization for three months at 8.degree. C. in a cooling chamber. Subsequently, they were transferred back into the greenhouse (25.degree. C.). Shortly after transfer, already after 11 days, the control plants as well as the mutant lines began to grow shoots. After 4 weeks all plants began to develop flowers.

[0096] Verification of the flowering and bolting behavior of double mutants:

[0097] F1 progenies of a cross of AP1 mutants with FUL mutants have been analyzed for identification of plants carrying the mutated AP1 gene and the mutated FUL gene. Two F1 plants could be detected which then were selfed to generate a F2 population. Selected plants of the F2 generation have been tested in greenhouse as described above. Shortly after transfer, already after 10 days, the control plants and most of the selected plants began to grow shoots. However, a few plants showed surprisingly no response to the shoot and flower induction by vernalization. These non-bolting plant were all homozygous for both of the identified point mutations in AP1 gene and FUL gene, expect two of the plants which showed a heterozygous genotype for at least one of the mutation.

Sequence CWU 1

1

42121668DNABeta vulgarismisc_feature(9833)..(11385)n is a, c, g, or tmisc_feature(12467)..(12605)n is a, c, g, or tmisc_feature(13741)..(14088)n is a, c, g, or tmisc_feature(16396)..(17194)n is a, c, g, or t 1atggggagag gaagagtgca gctgaagagg atagagaata agatcaacag acaagtaact 60ttttcaaaga gaagaagtgg acttgtgaag aaagctcatg aaatttctgt tctttgtgat 120gctgaggttg ctctgatcat tttttctcac cgaggaaaac tctttgagta ttcttctgat 180tcttcgtaag tatatatata tatatattaa tagtaactac ttgttttctg ctttctattt 240ttaggtctga tgcatattta atttaggtaa tattaattcc ttatatctga tccttaattt 300ttttttcttt taccatttca tttttgtttg ttttgaataa aagaaaattt ccccttcacg 360tgtgtcgaat aggtcaaaat ttttacttga aggatgttct ctttgattac taaaatagga 420tccaacaatc acctgaaata aaggaagaag atggtgcaaa gtttttactg tcatacttag 480tatttgataa atattctatg atgaacttgt ataaattagg aaatagacct aactttcatg 540cacgaaaaca ttattccttc attcaatttt tttattactt aaggatttac ttttttattg 600atcatatgaa gtagtagtac ttgtaatcat tcaatttttt tgttggttaa ataggactac 660attttaaaac aacccaattt taaaattttt tgtgtgaatt tcttcccttt ttaaaaataa 720agtctattat catagcttag agtagctgtg gcaaagctag acgaaataat acagaaatct 780ggaaaggaaa ttgtactact tacatgaaca cacttattta ttacttgcat gatatctgcg 840aaaaagttta tagcaaattt ggttaatata tagcgtagta ctttggatat taatattact 900agtgtacaaa tacttgatcc aatgggtaat gaaacttatg gaagatttga ccatacatga 960tgatgctaaa tattaattgt tattgtccag ctttgttttc cctccatcca ttggcatctt 1020catctttaca ttgctactcc actcacttgt caattgtttc gtcctttatg ttctttattc 1080acatgtgcac catacttcaa tactttcccc ttctttatcc tcagtttttt tttcttgtca 1140ttttagggtt aatatccaat gaaatctagt ttgctcgttt tagatctaat tttaattcga 1200tcacaaccat ccatattttt gtttcttagc ttgacatcta ttctatggat ctgggatctt 1260cggtgtatag atgttctcgg ttttcagatc aagatcctat tcatagaccc atttattgta 1320aacacttaaa tgtgttctta aaaagttagt ggctcgccaa gtcaactcaa taacataacc 1380cccacgactt cattacatta cacaatgaaa gattagatgt atgagtttgt gaagcttata 1440attctatttc aagtaggact aggatgtttt gtgcaatcag cagctagtag tctttttaat 1500ttaagtcagt cttcattgtg catcatatat ttttagaaat atatgcaagt ttgaaaccat 1560ttagaacctc atgacccgcc tgactcacta taaaccggca agagcttaat ttttcacagc 1620tttgtatctt tatgagtagc gctagctagg ggtatgggca tagaaaaaaa gggtttgggt 1680tagggtctta caagatctta tccgctattt ttatttcata atctttcaaa atacatgttt 1740aataattcaa aatacatgtt taatactatc tccatttcac aacatatgca ccaattgcct 1800agctatggtc caacctagtt ggtttgtagc ttgcattgga tggttaggat gtattggagt 1860tgtttatgtg caatcaaatt ttaattacgt atcaaaaaaa aaaaaaaaaa aacatatgca 1920ccaatttcca tttggacaca cttattgacc aatttttgac aatatttttc tcaccatttt 1980gtaagaaaaa tcaaaatcaa gtggaatttt gttaagttta tctcagtcaa aagattccat 2040acatcgacat tttataattt ttaatcatac gcaattagaa atatcaatgt ctaaagaagc 2100gtgttggaat acgtgaaaaa gcaaatgata catgaaacag atgtagtata tagaaaactt 2160aattttgtgt cactcggatg tatgtgggcg gagccttcct agaaggcgta cccaccttag 2220tggctctgaa tctttgacga cccgttcggt tggtggtgat aatagatggt aatagtaatg 2280taatttagtc taaatttata aataaatatt aatatcatta cccatggtaa tacaagttct 2340tcacaaaaca tgtttcattt aaaaattatc attactacct tttcaagtgg tattggatga 2400taataaaatt ttaggcaggg aaatgggtat tgggatgaac attaccatgg gtaatgacat 2460gcaatttttg ttacaagaat acagtataat acattactat tgccaccatg tataaccatt 2520aatcaaatgg accgtgagga tatgatgttg aagaagaagt cttaacctct acgctattat 2580ttactagggt ctgtaaattt tcctttttta attataattc ttgtgaaatc ttcttcactg 2640atggtactag cttattagga tgggtttctt tagtatattg aaggctcttg ttgacagagt 2700ataaaaatat ttttggggtc gcaaccatca atttaaactt ttgtttgatt ataaaattat 2760tttttgaaca tcaacaatct acttaaattt ttggttgagt tagttctttg acatggtatc 2820acaaccatca tgacataaag gtctcatatt caaatctcat tcacctctca tttccaagta 2880gaatatttac ctcaggtatg ggtatgaggg aggcttgtgt tgcatgagtc aataacggat 2940cttgaccaat aatttaacag gggcgagttg ataaattaag ttttaatgta aaattttaaa 3000tgatggataa aaacactaat acacaccaaa atataaatat acttttatta atggttacaa 3060agagcttgta gctaatgtaa taaatcaaaa tcccaaaggt gcaattttta agaaattatt 3120tccatttatt tatttgacca ttatgaaatc ttcaagaaat tgagtaagtt tttaagaaat 3180ttaaggtata gttcattaac taaataaact actccagtaa aaaaaaatta ccaaactgct 3240ttcttaagta aaaaaataaa taaatttata ttttatgatt gttaggaatg agtgtgagga 3300aataaaaagt actattatag ttaaataaaa atgaaagttc ttcagagaag aagaatagaa 3360gatagtacaa tcaatgttaa atatttttct aaattagaca aattgatata aaccaaaaat 3420aaaggggaag aagaaagaaa taagtaaaaa aagaaagaag gaaaagaaaa aaagaaaaaa 3480gagaagcaag tgaagaaaaa caaagaagtc caaatgtgtg ttgatgcaag gttcgagctt 3540gcaacattaa gggctcaaac tttcttttac actttggttc actgccaccg tgcccacagc 3600ttgttatgtg acatgaagtg tagtttgctt aatttatctt atacagttat gggggaccaa 3660gcctccaccc gccccttcta taatctgtca gtggttgcat ccacacttta agtccaatag 3720actcttgtct gagaggaggt gatagagtat ataaatattt ttggggcctc aaccattagc 3780tcaatctttt gattaagttg gttctgtgac acttgtacta tatactagtt atatatatac 3840tgtaaaacta gtaccacgag aacagtcctt aatacaaaca acatgccctt aatagaattt 3900tcttagtata cacttaatat aggttgacta gctttttgcc cttcagtatg cacacacctt 3960ttataatctg tatcgttgtc tggtagatga taataaacct cagtattggc aatatatgaa 4020atgacataat ggccatgttt ggtgattaga gtttagagtt tagaggttac agttcagagt 4080ttgtggttag atgattactt ttttgttcag aggatttgac tgctgattta aataattgtt 4140gtgtaaaggt gtttggtaac acttagctta ttgtttagag ttttgtactt tttagagcat 4200gtaaaatgac atttatggac atatgtattt ttttaaaaca aattttagta gtaattatat 4260ggacaaaata gtcatttgtt ttttctctct ccaaaactct catgaaaaag ctcctctacc 4320cagctttttc aaaagagagt tttgatcaga gttttcggta caaaactctc tttagtcctc 4380tctctcacca aacacccaaa ttagagtttt tattggtcaa aactctaaac tctctccaaa 4440cctctaaact ctctctaaaa ctctctcccc caaacacccc caatttctta gaaaaatttg 4500ttgctccttt ttattgcact atatttctat ctccaaacat aaagtttctt ttacaaattt 4560tcatttctac tccataccac ctttatatgg caatataatt tctatgaatt aaaatgttca 4620caagttttga ggtggatttc aagagcatgg acaatatgat catgagactc tccatacaaa 4680aattaccctt aaattttata atcatacacc aagcggtcgt taaagtattg gaagtgcttg 4740agtagtttgt gaaaattaac atataataaa gtgcagatct cccctctagt aagtagtaag 4800aagtagtaag acgatgtccc tcatttgaga aagagaaaaa cccttatcag tttctcttgt 4860ttctttgact gaacgcaagt caaatagaag tatgtaacta ggaaatcctt ggagaaatag 4920attttcttta aaactataaa agtataccta tatatatggt aacccacaaa aatgtatata 4980atctgatcaa tatctaaaca aagtattctt atgttttctt tcatcttgct tatttcctcc 5040ctttcctttt cttactttaa tttgtttact ctctttaact tatttctttg cgtatctcat 5100ttcactttac aaggatatat agttgattat gacagcttaa taaatatatt ttggaactag 5160gatttattgg ttgtcgttgt tattttaatt tctacactga tcggctagag tttctagaac 5220atagggcttt attgaaacca ttagttaaca aaattgaatg acaatgattc aatatgatag 5280aatatgtatg tattagttaa tgtttgatta ttgtttgtat gtatataatc aaagattatt 5340tagtaatact tctatataca tattctatta gaatcactta gaaagaccca ttgaacaata 5400ataaggatag gcagacaagc aaacaaaaga aaaataaacc tgttactcct tccatttctt 5460aatgttctac tcggaattat agatacacac tttgacacaa attagaaaga gagtgtaaaa 5520agtggatcca tattaatatt tttatttttt taaatgagga gagaagtgtg ggtttattat 5580gtttcaaggg agatagagag cattgaatag tgagagaata tgtgccaaag ataattaaat 5640cattgtaaat cttttgccaa ataaagaata aagcatgtga gtaaaacttt aaaaaatggg 5700cgaaaaagga aagttgagta gaactttaag agacggaagg aatatagaag aggacgtgac 5760agatgggagg aagatcagac atcttagaag gggaatagtt aaatttgaga tagtctttta 5820attaaggttc tcactaaaga agatataaca gtaggggaaa gctaaaggtt attcaaaact 5880ttccttccca tcttcatcac ttcatgtctt tactttagag ctcttaacac ttagcctatg 5940aaattctgaa ctctttgtaa gattagtgat agataaaaga atcttatcaa tttaatttat 6000aaatacaaca ggattcaata aaaagatata gagatctata aataaagagc catactgttg 6060tgaactttta tatctatcaa aacctttgca cattagacgt ggtataacta aatcaggctt 6120atcgaaattt tttaaaattg ttttcattat agccccttta tatttagaag ttctaagatg 6180attgcataga tagttgatgc accgttctgg tcgacttttt taaacacttc tttttgataa 6240attttttttt ttgtattcga atcattattt taggtgtata aagagctgca aatgatctag 6300atgagattga tctcggtttc atttatatgc taatagtgtg ttagatacac actattaaaa 6360aagtcatatg acttagagat tattatggaa aagggatagt gcaccgatat taatataatg 6420gaaaatgaca cacgagttgt ccataataac atgtgaaaag tgaactattt aaaaggtttt 6480tctgacctag tacatacaag gtgcgtaggt ttagctattt tagtttttta gttttatttt 6540ttaaagtgaa gttagttatt gatctgaaat catataacat gtacgtaccg tagatataaa 6600aaactaccaa gtatatatca atttgaaata aacattattt taatatggca aaatcacaat 6660tgttgactag acctaacact gaagaaaact atgtcatgtt tatcaattat gttgcataca 6720gttaaaaaca aatatgttag agaaatcgtt atttgaaata gaaaagttgc gcaaaatagt 6780gattaacatc aaaatatgtt cagaaagttt ttataaatat gtgatcttgc attgtctgtt 6840gactgtcgag gtttatgata atttcccctt tttccaatgc aaaacttgtt gtgctatttt 6900ctaatgatat attttttcaa agtatggaga agatcctaga aaggtatgag aggtattctt 6960acgcagaaag acggctagct tcaaatgatc cagactcaca ggtagtgcat ttatgtaaat 7020atagatatac tcttcatgcc caagaagcct gaatttttta tcccactacg tactgcaaag 7080ccaagtttaa ttgaataatt gtcctgttta aattatttag ttttcagtac aataatgtaa 7140tcattagttt gcatgtttaa aaaagaaaag cacaagttct gatcaagtga aatataaatt 7200gtaacgaaag agccaagcta gacaattacc tagctaggag ttatttgtta tcgtttttgt 7260ttttaatttc tagttttttt ttttaaacta gaaaatatag tttcaatctt ttgttatcag 7320ttttcaaaat gacatattta acataaatat gattgatttt aaattcattt attatatcat 7380atttcatttc aaaataagtg aaacacttgt ctcaaaaagc tcactctcac ataaatgata 7440aaagtgtttc acttatttta aaacggaaga attatgactt ttacttttca taaaacgaaa 7500aactgaaata tgacaataat ctcaaatagc ttggagaaac cagatttcta tatatttccg 7560tgatgaaatc acttttcatt atacgtaggt aaactggacc tttgacttcg caaaactgaa 7620ggcgaagctt gaacttctac aaaggaatca taggtatgat ggcaatatgt cataattttt 7680ctattattat ttttgcttcc aaaaccagac catatgtttg tatatttata tagtgatata 7740ctccatccgt ttcattttaa tctatacatt tacacttatc aggtatgtca atgcaaaatt 7800ttgaggatat atatctttag ttttgtattt ataaaaatta taaaaagtac atattaataa 7860aatacatatt atgatgaatc taacaagatc ccacatgacg atatttccgt ccgcgtatga 7920ataacaaata atggccaaag tgaaatttgt gaatagtgta aaatatcaaa gtgtaacaat 7980taaaataaaa cggagggagt agtacttgtt tgtcacatac ttacttattt ttgttctctc 8040cacaatgaaa ctgttctttc taataattaa aaaaagtgca tatgttgatg atttctctgt 8100cactttaagt ggatattgaa tagtgataat ggattacttt gtgtataatt gcatttcaca 8160tttgggtcta attttatacc cttttcgcat atcatgcttt gtgaatagta catatgatgt 8220tcaagaatgt gagaagacat atcatacttt tgatatacct caaacatggg tgtatactgt 8280atagtgaacg aaagtgttag tgtaatttta tttaggaggt ttagtggttt gtcctatata 8340taatgctagt agttatacac catagttgtt gatgagcatc aactggcttt cctaacattt 8400ttttctccat aactttaccc ttaccttaca ttagattact ctaggattac attctaccta 8460aaatattatt actcccatca ttttaggtaa atatttttac tttgattttt cgattatttt 8520caagagttta aaataatgat taaaatttac catgatcagg cactacttag gacaagagct 8580tgactcactt aacatgaagg aacttcagag tttagagcaa caacttgata ctgctctcaa 8640aaatgttcga tctaggaagg taagaaattt tacttgtcta ccgtagtttc ataataaatt 8700agtatttggg ctcgggcttt gccccagatt ggtattgtct tttcaaattt gatatgcatt 8760tttttccatt tccactaaaa tatattaaga aaattcaaca tttaaaggat acaaatataa 8820taatgtggat acttaaagta tgattaaaat ttggttgaga tggtaattgt gtcatgtata 8880atagcaagaa gtcacaagtt caaagctcgt tgcaagctaa atttattttt gttgattgac 8940atgacttatc aacacactgg acaattctaa tcatctagtg gagtagcata tactagcaat 9000ttatgcacgt gatgtgtgcc ttactttttt agaatataat ttataacttt tttgagcata 9060aacaaaggta aaatttgaac attagacata tttttttggg ctagctaaat ttgttgttta 9120aacctatatc acttaaccaa actcctcttt tattatttat tgatttatat tttatttaaa 9180atttttaaaa ttaaaatgat gagcaataaa agaatgttaa gtagatttat taagtatttc 9240ttatattttt atcaacaaag tattttgtgt taattaaatt atttcacttt gttaattgat 9300tgtattttcc tttttaattt attacttgat tgtgtattga ttgatcaaac ataatttttt 9360tgttaatttt tttatgctat atttgaattt atttttcttt catctgtttt tggtagagta 9420gttgatttac taaagggtaa ttaaataaat ttattggggg acaccatagc tcccccctcc 9480cttatataat agagatttgt atagatttat tgtcttcctc aattattgat taactagtct 9540tctatgcacg cgatgcgtgt gttgattgtt tgggtctatt cttaatataa atttcatcaa 9600aatataatta tagtagtgtg atttacaatt attgctatac aaactactgt aatttataaa 9660gttgttagaa attgagataa aaatttagat gtgaaatttt gtggtcaaat tatatttgta 9720attttttaaa ctgagtaacc gtttttctca tcatgtcaag ttactttgtt aatgcttatt 9780taatttatta ttggaatttt tgacccatct ttaaattaga aaaggatata atnnnnnnnn 9840nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 9900nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 9960nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 10020nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 10080nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 10140nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 10200nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 10260nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 10320nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 10380nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 10440nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 10500nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 10560nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 10620nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 10680nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 10740nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 10800nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 10860nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 10920nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 10980nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 11040nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 11100nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 11160nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 11220nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 11280nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 11340nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnntatat atatatttag 11400ctaagaaaaa aaagacattt cattggggga taccataact cccccttagc ttatataata 11460gagatactat acttcttttc tgatagtgtc aaatttaatt ataaatcttt aacattggcc 11520aattaataat tggacaagaa aaaaatgaga caataataaa taaggcgatc ttcacagacg 11580tattaacatg atggtaatta aaaatgttaa tcatagatct ttgtgttatc ttaataatat 11640aaatttacta attagaatgt atcacataaa gtaagtatta atagcagcat aggataattc 11700ttataatgga gattttatat ttttttatat aattatatga tttattgttg aaaatattag 11760ttgattttaa ctggttgttt attcaatgac agaaccaact gatgcacgag tccatttctg 11820aactccagaa gaaggtaata actccatttt ttactctcaa aggtttattg tttttaactt 11880atttcttcta accttttata tatgagaagg tattgggtta gacgcgtctg accataatat 11940taggtcggat gactttcagt tggtttcaat tttatttcag ttggtttcaa tttttgtcca 12000gttggtttca atttttgttc agttggtttc aatttttttt agctggtttc aatttttgtt 12060cagttggttt caatattttt tagttgatct tttttatttc agttggatgt cttttaagtt 12120cagttactta tcttattgtt tcatttacgt gttttattgt aactgaaaac aaaacttaag 12180taaatgaaat aaaataagtt ctaaataaaa gcaacttagg gcctgttctc cccagcttat 12240tttcagttca gttcaattca gttcagttca attcaattca tttcagttca gttcagatca 12300gatcagttca gttcagatca gatcagttct tgacaatact tttactctca catatcacta 12360ttcatttcag ttcagttcaa ttcaattcag ttcagttcaa ttcagtttag ttcagttcag 12420ttcagttcaa ttcagttgtt ttatgccgaa gagaacaggc ccttagnnnn nnnnnnnnnn 12480nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 12540nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 12600nnnnnattca tttcagttca gttcaattca attcagttca gttcaattca gtttagttca 12660gttcagttca gttcaattca gttgttttat gccgaagaga acaggccctt agttttcagt 12720tacttatatt atcgtttcag ttagttttct tattctttca tttaacaact aactaaaata 12780aaaaaaaaaa aactaactga aagcaaaact taattaaatg caaaaaatta agttctaaat 12840gaaaccacat acgatcgaaa tttcaatcat ttcaaacatt atggtgtttt cgattctttc 12900aaagaaggca agctgctccc gctattctac cctctttaga tcacaataaa gctcaggcct 12960cacattcaaa gtttcctcaa agatggacgt tccaagtatc acatagacac atagtcctct 13020tctccaaacg ctctccttcc tatcttgatg tcattagcaa acttcttgat ccagacggcg 13080ccaacaaccg caccatgatc tccctctaaa gtactgacgg cccgtttggt tgttggtcat 13140aaatgatggt aatgggaatg aagttgtgtg taaatttgtg aaaaatatca ttgtccattc 13200ccatggtaat gctaatttat cttaatgtgt ccactttcct tctagaattt tcattctcat 13260ccaataccac cttgtaaggt ggtaatgagt ggtaatgaaa attgcttccc cttggagaca 13320aaaatacaag tttaggagtg agattgattg ctcatggaga aaaaaagtct ccccatggag 13380atattaaggg tgattcccta ataaaattac acttaaaatt tattcccatt accgcaattt 13440attaacatct accaaacggg ccgtgaaagt cttgaaacac atagtcgagt gagtagcttt 13500gaggaaccat ctgtaaaaga acctgaggga gccaatgtgt gcgtaagtac caacggcgtg 13560ttgtcagtgg aaaaggtggt gccgtggtgg cactcagtag tgatggagcc gccgtggtgt 13620ttgagtgttg ccaaatacaa aggcggaatt tcgtaatctc taatttcttc tgtgaaattt 13680ttgggatcag cctgtccgac caaacacatt ggatcaaacg gtctgaccca atagtttcaa 13740nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 13800nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 13860nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 13920nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 13980nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 14040nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnga tataaataga 14100gatggaggac aagggtcttg gttttgtcat gttgtcaaag agttgaacaa tggttttttc 14160gtattgttaa aaaattaaaa aaccagaggc cttcaatctc ttgatagata tagatagaaa 14220aggaggacaa ggccgttctt ggttttgtta tattgtcaaa gagttgaaca atgatttttt 14280cgtgttgtca aaaaattaaa caatgaaaaa agatggcggg tgcttgatct aatagatcgg 14340accatggatt gaaggtcttt taacttattt tatatatata ttgaacttat ctgaagatta 14400tttaactctt tgaaattgta ttaacttccg aactttatga acttttttaa ttcttcaaaa 14460cttatctaca ttttatttga aaaaatattg aagacaaaaa aaccctcagt tggtttaaag 14520ctgcggtaag atagagtgta aatgttattt ttttttatta aatcaagaaa taaaaagaaa 14580tattaaataa aaagaattaa aaatggaaat gatgacagaa acttatggct tggaggagca 14640atacttttaa gatagaccta aaccttaaat aagttaaaat ggaagtaatt tttcagtaga 14700atcttattcc aatctatact ccgtgtttac tccatgtaat gcacatataa taaaaaaatt 14760agaaattaca tagtataagg tttgatcctg tgactgtaag tttatatact aacttcttaa 14820ccactagagc aagtgatatt

tagtgttatc attttaaagt ataattttaa caaatgaaat 14880ttttttctta cccggaacat agctcggacc taataactag ttgaacaatt ataatctgta 14940acttaaaatg atcctaatta ctgtactttc attacctata ataatagaat cttactatca 15000ttggttcaga aaaaaaaaat cttattaaat gttaaccatt tatttgtaat tgaaacatac 15060atgcacataa atgtaacttt tagtttatct taacttaaaa actgagaaaa tgttagttgg 15120aaacttttgt atatatgttt ggataaacga cgctcaaaag taggggctaa aattttagta 15180gataatataa gattatactc catctgttct agatagactt ctcattttta attttggcag 15240tattcataaa taaaggaaat ctttcaaaaa aatttccaat atataagaaa aaaaataatc 15300atgtgcggtt ttgtttgatt cgtctcattg tgtacattag gaaaattaaa cttatataat 15360ttttactact atgtaattaa agatattaac gatacaaaat gtgtattgac aaacttatat 15420tggagtaata ggaagtctat taagggaccg aagaaatatt acgtaaataa atctaataca 15480aactaatata aattctactc cagacaataa agattctgtc ttatattgcc aagatatagt 15540agctatttat tttatcttaa caaacataaa tgtttctaat gcttaaacat ggacatgtat 15600tattttgtaa aatattatgt attatccaaa gttacatatt taaaggaagt tctattgctt 15660gctctctttt agcactgccc aaaaaggtta aagtaatttt ttttctctgt ttaaaaaaaa 15720aatgcattat atacagataa tttttgctag tcaataaagc tatccttatg acttatgagt 15780gctacttgac tagggatgtg ttgtactcaa ttggaggtat acatacacca agattataga 15840gcttttattt tgcctataaa aaatggaagc cggataggat accaaaaaag ctttgactta 15900aatttgtaat gcataaaaat gatgatacct aacttattag ccatacttat ctaagcgtac 15960gtcaatttaa atattgtgtt attgattaat aatgatcctt atatatccat attttgacaa 16020ttaaacggta aattagagag aaaagtttga gaaaataatt atagcttacg taatgctata 16080atccaaagtg tctccgcaca agcgtgggac aaaatagtac tttcggagaa gttacaatca 16140acagctaggg agtcttcatt gttcttgaat agaaggatgg aaacaaagtt caccttcttt 16200tattaaagta ttaaggtttg ttattagctc aatatccaat actttctctg ctttttatta 16260cttcgtctgt ttcaaattaa atgatttttt ttttatttta cactattttt aaatttcact 16320tttaccatca tttatgattt atatgtgaat gaaaacatag ttacgtgtga tcttgttttt 16380tttttttttt tttgtnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 16440nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 16500nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 16560nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 16620nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 16680nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 16740nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 16800nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 16860nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 16920nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 16980nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 17040nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 17100nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 17160nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngtacat caaggaaatt tgcattaatg 17220aaaaacggga gtacaaccta atggtaatac aaccaaacta aacagaaaga agaaacaaca 17280gacagtaaga aaacctctat aacgcgtaaa aacaatttaa cataaacact aactagaaaa 17340agtccaggcc gaataacatt tgtcttgtgc gatggggagg gaagatgaag gagagtgaaa 17400atctgttgaa agacacaact ctgacgatct tcatagagag attgtcgtac aactgctagt 17460agatcctgtc cactaggcac gaaatcccaa ggtccttgat gagcctcctg ggattctcct 17520gattcgttga acaaagaagt cactttcgag agtaacttcg gaccctgctg cgagggtctt 17580cgaaaatcag tcacattaga ctgaaacccg aaaaacaagg tggaggtctt gacgtgcccc 17640aaaacaagtt gggggcgtcc cacaagatgc gttttttagg tgtgatgaca catgatgtca 17700tcacgagaaa ttggggcgag ttagtttgat gaactacgcc ccactgacgg atcctaagat 17760ccaaagtgac aatctcgaaa ccagaagacc gacaaacaac agatctgaaa catacaaaca 17820tgaaaataat gaaagcataa actgccaccc gacatataga gctccggcaa acaacaccat 17880caagaacttg caccaaagac ttccttggca tactaaagac actgattcca actaacacta 17940gcgggggacg gggaagggac actcgactac acctaaacct aaccagggga cggggaaggg 18000gaacttagac taaaccttcg taaaaagggg gggatcgggg aggggaaaac cttgaccaag 18060gaagctggtt ttaaaaacca cttagccgag ccaaaaaccg tgggtgggaa gaagaaacag 18120accacaaaca gggggaaccg ggggatggga actcaccgaa caggggaggg ggagaaatcg 18180cacagactcg gggaacgcct aaggactggg ggacgaccaa cgaacgaaag gttggggtgg 18240tgcgaaaaca agggaggggg acgcaccgac gaacaaaaaa accgacgaag aggccgaaaa 18300agcgaaaggc cgacggagat aagattgaaa ggcgacgaaa aaagaaaaag gaacaaaacg 18360aaagaaaaac gaactcgtcg gagacccgcc ggagacctac gcggcgccgg atctccggcg 18420agttctaggg ttagagggtt tgttgtgttt gtttagggag aaggcagagg tttttttttt 18480ttacgtgtga tcttgttaga tttgtcttaa catgtattct ttaatatact ttttttttta 18540taatttttgc aaatgcaaaa ttagagatat atgtcctcta aattttacat tcacatacgt 18600gataaataag agtgctacaa ctaatttgaa acggataaag tatttgaatt gtttttcatt 18660taaaaaagtt cgctatcatt tataatgtta tatatttgcc aatatgttat ctctttctct 18720ctcttaccag agtttagatc cagtagagtt agtaaataat tctaccacgt agagttgaac 18780aaatcatagc cattgatttt caaatcattg gtttatatat tctttcccaa aactcccccc 18840tattttcccc aaaaatcctc cccctcctta tctctttcca taaaatctga gtcgttgatt 18900ttaaaatata aggtttggat tcaactccac tatgtagagt tttcatcaaa ctccaccgaa 18960tccgagcccc tcctaccata gtacttcttg atttccccat atttctttcc tcatcttggt 19020cctcaagcac attttaatat tatgggtatt aaacaataga gaaagtattt acttatagag 19080aaagtatttt caatgattcc ctaaattttt ttttgaaaga aagaaaaggg atttcattaa 19140tatttcgcca aacggcactt acaagtcatt tctgaaaaac ataaaattct aaaagaaata 19200catatcaccc tagaaatgta aacatcgcag atttgactta attttgcctt aataaaaatc 19260ttcatctgaa gcaatgcaat ctgtgagttc gctctggttc ggcatacgat ctgcagatgc 19320ggaaattttg ggatgaacgt actccaatag tcttccataa ttttacagaa gttgtgaaac 19380cctaattctt catgttgaat ctcgaacttc aaccaatgag aataatttct catacctaaa 19440aacaaaagaa ccatactcac aactcccata ggggagaagg agatttccaa aacagaaact 19500aaaaacccca taaaagggtt tgagaaaatc tcataaagag atactaattt attgaacaaa 19560acaagaaaat gaactaaaaa ctgaaaataa aagggaaaaa ggggcttacc atggatgaaa 19620acatccatgg cagcccccta attgatgaag aaggggtaag ggaggctagg gttttagaga 19680gagaaaagga gaggggaggc taggttttaa aaaaaaatat aatgattccc taaatttact 19740tatatatatt taccaagatg acgtgatgtt ttacaaggcc catgattttt acgcgatcat 19800gaaaaacaca gccaatttga atggagcaaa tatctacgcg tcattttaga tatttttgta 19860tgggaaagtt ttttttgacc aatgtaatta ttaagaagca tcggccaccg ggtagataag 19920atgtcactat acatcctttt ccaaacttaa gtatgcctgt tgaacttttg ttgcgtttgc 19980agattcattt gaaattatat ttcctcagat cctctacttg taaaagaatg ttccattatt 20040ttcttagttt acatgatatt tacaatagta tttgtctaca ttttgttcat attacttagt 20100gatcagtgta tacgtcatat attagtttga actttgaaga catttatttt ctatatactt 20160cctttgtctg ctaaattact ttggaaagct ttgttttttt tattaatata agaccctttg 20220gagtttggaa atcactatct aatgaaatat ataattcatc attagaacaa aaatacaaat 20280atcgtactat cacctatcat gttccttttg gatttcgctt cacaaaaata cattttaaaa 20340aaaaataaaa taacaaatgg tagctaacaa cttattactt ttaaaagttt gtgtgcaccc 20400taataagtac tcaaagtagt atgtaacaga gagagtataa tgctaaaata caaactaaat 20460aaacaagaaa gtgtttctca acaataattt gctgcaggaa ttaggaaaca aagtaaataa 20520attgcatgtt tatcatcaat acaatttact ggtagttaat tacaaacttc actcatgata 20580attgaaagag gccactcaat ttcagctagg agttgtttat ttatttattt ttctttcagt 20640taaattttga ctacccacaa aatcttcatc tggacctaat ctgcaatttg tggattttgg 20700atgaaatttc taacctattt aagtagtctt attgtttaaa taacccatgc aattaaatta 20760ggttatatgg gggtgattca tttaccaggc ccaagatttt atctcattct caattattat 20820cgcaacaccc atgaacctaa gccaacatga cttatttacc aggccagcta gagaagaaca 20880aggttgctga ttttcttgtc cgtgattgta gaagaaatgt tagaaatcta aatgttgtta 20940gggatttacc cctcccccct actgagtgta tgaacttatt attgacggat tgttgtaggc 21000ttccaagcca aaactctgat taagttttct tttatgccat tttaaccaaa aaaaaaaaaa 21060aagctaggaa gctagctcag cgcgctctaa ttatttcaca tgtgacatgt tttacactta 21120ttcatacttc tatatgcagg agagggcaat gcaggagcac aataacatcc tgtctaagaa 21180ggtacttgca cttgaccagt ttgtgtaata ttgtaattta atttcttaga ttttggttgc 21240atgctttgat gacgaatgac gattgacgaa tacattttta tgcagatcaa ggagagagga 21300aaaaatctag agcaagtgca acagatgcag tggcagaacc agcaccagca ccagcaccag 21360cagcagccgc caccgccgcc acaaaatcat caagttcctc ctgatgcatc aaatttcatg 21420ctcccacctc caattccttc tttgaacacg gggtagttac ttcttcaact taatttcctc 21480tattcaatat taagttaaga aacagatcac gtgattagtt cgttaatatt gctaattaat 21540aatcatattg ttatatatca tgcattagtg ggtaccaagg acaatttggt ggagaagtaa 21600ggaggaatga tcttgacctg acgctagaac cgatatactc atgtcacatg ggatgcttta 21660caacatga 216682780DNAArtificial SequenceBvAP1 cDNA 2atgggaaggg gtagggttga gctgaagagg atagagaata agatcaacag acaagtaact 60ttttcaaaga gaagaagtgg acttgtgaag aaagctcatg aaatttctgt tctttgtgat 120gctgaggttg ctctgatcat tttttctcac cgaggaaaac tctttgagta ttcttctgat 180tcttctatgg agaagatcct agaaaggtat gagaggtatt cttacgcaga aagacggcta 240gcttcaaatg atccagactc acaggtaaac tggacctttg acttcgcaaa actgaaggcg 300aagcttgaac ttctacaaag gaatcatagg cactacttag gacaagagct tgactcgctt 360aacatgaagg aacttcagag tttagagcaa caacttgata ctgctctaaa aaatgttcga 420tctaggaaga accaactgat gcacgagtcc atttctgaac tccagaagaa ggagagggca 480atgcaggagc acaataacat cctgtctaag aagatcaagg agagaggaaa aaatctagag 540caagtgcaac agatgcagtg gcagaaccag caccagcacc agcaccagca gcagccgcca 600ccgccgccac aaaatcatca agttcctcct gacgcatcaa atttcatgct cccacctcca 660attccttctt tgaacacggg tgggtaccaa ggacaatttg gtggagaagt aaggaggaat 720gatcttgacc tgacgctaga accgatatac tcatgtcaca tgggatgctt tacaacatga 780329237DNABeta vulgarismisc_feature(1029)..(1924)n is a, c, g, or tmisc_feature(20703)..(20722)n is a, c, g, or t 3atggggagag gtagggttca gctcaaaaga attgaaaaca agatcaaccg tcaagtgacc 60ttctccaaac gtcggattgg attgttgaag aaagcgcacg agatctccat tctctgcgat 120gccgatgtag ctctcatcat cttctccact aaaggcaagc tcttcgagta tgcttctgat 180acctggtatg tctaatttta taacttcttc ttttgtacat caataatttt atcatcgact 240caactaaaag cttaagcaga tggttagggt tctattatta ttgaattacc tcaaatttgt 300catcgactca actaaaagta gagtatattt catgtagatc aggtgctttt tttgaatata 360ttgtcagttt tagaactaca aaatgttgaa cacaagtatt tatacgcacg ctgacatgtg 420aattttttaa ttgacaactt tctaaattaa tactctaaat tactaatatg aagaacgtaa 480tttattattt atcactttca gacaaaggca tgtttgtttt ttctattatt tttcccatga 540aaattctcac caatatccga ttctgtatgt taattttagt aatttctaat tttgatgact 600taataaattg taaaaaagta taaaataaac aaatatccaa aacatctttg ttttcaagag 660aaatatctta aaaacttttg ttttttaaga gaaatatctt aaaacacttt ttatcatact 720actatgatga tgtataaatc tattcaaaaa aaaaaaaaaa tgatgatgta taaataattt 780aaagagttaa gtttattaga aattatagat atttatagag ctgagtaata aaataatact 840ctacagatta tatgtagctg atgtagtgtg tctgctcctg taagatttcc tttttatctc 900caaaaaaatt gcattgatat tcgagccttg ccgaccccct tttcctcttc aaccatttga 960taagatccta tgcactgagt aatctagtat tatatgttag atgttatata ttaataagct 1020aaaattgtnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1080nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1140nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1200nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1260nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1320nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1380nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1440nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1500nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1560nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1620nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1680nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1740nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1800nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1860nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1920nnnngatgat attatgtatc attattatca gtcatttatt attgatagat aatgttatta 1980tttccataga tcatatataa tcttaccata tttactccct atgattatat atttctatct 2040attaacatag taatagtgat atactaacaa cgagtatctt tcaagtaaat aaatcatata 2100tatagggtta gaaggtaaaa aagacaatat tttatgagac tttatttaca cccttcgtct 2160cataaactcc tttctatttt ttgtgttcac tcgttttcta aagttttttc tacttctttt 2220actttttatt ggataaatac tttttccgtc tgtatgtgat ctacttatat tagtatttat 2280agatagatta tactcttaaa gtattacatt cacaaaatca tgtgaattat taattaaagt 2340aaaattatga gtatggtatt ataacttaaa cgaagtagtt gtattgttta aaccaataag 2400tataataact tataaaactt aaaagttgat tctacactta tcatgcactt gtgttttgat 2460gggttaaaaa ttagctagta cataagtaac ataacataaa cttatctctg tatagtatgt 2520tgaattatta ctttatattt gaaaagaaca aagacacaat aagtccaaaa gatccgatct 2580tttgattatc aactatgtaa gtgtctttcc taaatgatca atcaccttaa ttagtatact 2640aaacaggaca attatgacat ataacacttt tctatttgta caagttaatt aacccattga 2700cattcattca gttagcctac atttttcaat aggtagtcat catcattctt tttttaacca 2760atttttttac aataaccatt acctaccaac aacattttca aataatgaac tgaatgatgg 2820attccgtcaa ggaaattgtt cgtggacagt ttgtttacct cccaaatttc ctttaatctc 2880atgctttctc catcatccaa acaatctaat accaatcgtt ttctctaatt gcataaaccc 2940taattgttga atcctttaat cttgcctttt cacattgccc aattccttta gtatgatatt 3000ttttattcga taatccctga taagtaaatt cactaatcta atttcagatg gattgttgta 3060gagattgggg aattgaagat tttttgcctt ctttgttctt gtttacaatg aaggatgaag 3120tttcatggaa tattgaagag aaatttgaga aaagaaagaa agtgtgggct ttactgacga 3180caaaaactgt cattttggtg gtttttttca ctaaggcatg tttggcacta gcgtttaagg 3240tagcggttag cgatttgaca agatcaaaac gctacttaag aaaatgatga gtgtttggta 3300agatagtggt tgttgtagca ggtagcaatt agagtagatt atgagtagcg gttgtggaat 3360gttactacaa gtaacgtttg agatttagag gtagcagtcc agcaaagaaa cattgtataa 3420tagcataagg tagaattaat taaccaatgt ttttttattt ctttttcctt ttattgttta 3480ttaataattt tattttatgc caattcaatg tttattttac aatagcaaaa atgtaattta 3540aatatttatt ttaaacataa taaattttat aagtatttta gtaaaattgg taatataaat 3600ttttcgaggg ttgaatattt tcttagatgt atacatttct ttgaatagtt aaaaaatcat 3660atctcctttt tgtgtaactt ctttgaaaaa taaatcttga atttaactat tgaacgaaca 3720tatgaaatta ttgtagttca tttcatatta taataaatgt taggtattgt cttttacaat 3780ttcaactact tttggcaaac aattctgatt aaacagctat tttaatcgct gatcgttgac 3840agcaaccgct aacagctact agaaccgcta cttttgccaa acatgcctaa accaagtaat 3900atcggggatt ctttattata taaaaagtta ataatgtgat tatttgaaaa aaaggttgac 3960tttatatgtt gtttaaagaa aaaaaaacat ttattaatag aaaatatact atataggtct 4020ctatataccc agggcgaaat gaatgtgtat cttttcaaat gagtatgcgt acatgttctg 4080taaatgcata tttcatatga gcataatgtt tttactatta ttatgcacat ttgtgtttta 4140atttttcaaa tgagtatgta aggaaaacat gtattcttgg catgtcagtg ttagtgattt 4200ttgttgttat ataaatgttt tcgtgatttg tgaatgtggt acaaacattc atgtgccatg 4260gcgtttagca aaacttttct agctcacatg atgcttcaag ctaattgcaa tgaactaata 4320taagggagag gacattttac gaattagttt tacattgata gtagtttgaa gaagatagtt 4380taggagatag tttgttggaa tagaagctat gtgttagaat tagttatagt catcaatttt 4440tgaataagac tcattattat ttcaatcctt ctacctttta attactagtc caactctcac 4500tctttggtat taaattacac cattcctacg gactatctaa caactctaac cacggcccat 4560actttttctt cttaacataa aataatatta cgcttactaa ctactaactc ctatgacatc 4620taccttttca ataaaataac aattgataac tatataacaa cttataatct aaaagtaagt 4680gtcttgataa gtgtagagta ttgtgggacg aagggagtaa ttcatagcaa atactatcat 4740agcaatacaa tactaggaga ctaagatgtg agttttgaac ttcaaaaaaa aatatacgcg 4800acatagttca ccggaagaac tgcagcacaa caaatgcaaa tggggattaa atgaggagtt 4860cacctacatc acacacaaga gcgattgagg attttcagat ctggaagaag agcgaaaaat 4920caggcgagag cttcaacttt cggagtttaa tggaagagct aatgatgatt gaattattca 4980gttggattta tcttttgtaa gatgagggga tgaagttgaa gattgccata ataacccttg 5040agggcgacac aatggtggtg ggaatggaaa tatggaccac gaccgattta tgagtggaaa 5100gaattgaagt ctctgataca atgacgtttt ggtatatcat cggtgctttc atggctgtca 5160gatctaccga aaggaaaagt agtggtgaaa acaaatgtcg ttcaaaccaa ccacctttga 5220ctttcaatat tgaagtgaca aggaaaactg gcaccttagc tagtgacttg gtggagtctt 5280tgcgtaaacc ggtggaaaag aagcttatct ttgagtttta gtgtggtctt ggtgcttgtt 5340taagtggctt gactatttag gaggaaaaaa atcaattgtg aacaattggt gagtaaccta 5400aaaatcaatt gtgaacaatt ggtgagtaac ctaaaaatca attgtgaaca attggtgagt 5460aacctttaaa ccagactcaa gagtgggaaa gaggcggtta gacaagtaga tttagaggaa 5520gaggaggtag aaaatagatt agaatttttt ttgggagttt tcaaatgtag tggtatcggc 5580tcataacaag gtggtcaaat ggatggagag cttggtcgtt tgagtagtct catggtggtg 5640attataacta cgttggcgat aaacaagttc gctttaccaa gaagttgatt ggtggaagag 5700agattttcat aaagaggggc tcgtatagca acagctttgt gtggtctcag tttcattgtc 5760ggcaaagccg tgggtggtaa atatggtgat tttggagatt gttatgatgg agggagttat 5820ggtggtttca tgagacatgg atgctatggt ggtgctgtga tcggtggaga agaggagctc 5880gtgaaaggtc actctgatgg agcgggtgta atggtggtct aggttttttc ggcatcggag 5940gttgcgtatc tcgtgaaggt tcacatttgt acaccggtga atactatggt ggtgaactag 6000gatgacagtc aaggtgacca tagtagaaaa aaaaaacata aaccatgtag cttagatgat 6060ttgaaaaaaa atcatgtttt tggagaagaa tcttaaatat tatgacagag gcaaacttgt 6120cattgaccaa atagatgaca tagcaattgc acgtgtctcg ttaaaatttg aaaccataaa 6180aaattcaaat tgcacttcat atgcctttct tttggttgaa aacttcatat accctaatgc 6240gtcaatatgg ttctttttcc aaaaaaaaaa gtaaattatt tcggcgttag taaaagcagg 6300tccacctcca taatccattt tattaagcca actcctctac cctacttttg caacctatca 6360tttcttattt tctaaaatca tatcaaaaaa acaagtgtga acccaaaact aactatatta 6420tacctaagtc taatttcttc atccaatgtg ttcaacccca tttttcaacc cttccactca 6480taaacccatc ttctttcact cctaaaactt tcagctcacg ctcgtatcac ctctttactc 6540acatatcagc ccaaacgatt tcttattgaa tccactaaat tatgtatatc gattttttca 6600ccaagtactc cgagttttca aaaaatttac ctggtacccc caagttttca aactacacgg 6660gataccccta agtttcaaac taatacactc agataccctt aatgactaac gacattaatc 6720gccgttagtc attaacctta attttctaga tttcaaccta attaaccact aaccctaacc 6780ccaaccctaa ccctaataat aaccctaaac ctaaccctaa ccacccctcc ccaaccctcc 6840caccacccct gccccccatc ctccactcct gcgcagccag caggcccccc acccatttga 6900ttttaaggaa gaaacacgta tcagggaagg gggagaactt agctctgaca gcggcaacgg 6960accaccgacg agtctggact gtaggacggc agcgtcaagg cgcgagccgg ttgggctact 7020gcagttcaaa gcaaacaggg gaggggaatc gagccgagaa gagagctaag gaaagagggg 7080tgatgggcgt tgcggagatg gtgcgcatag tggtggtggt gtgggggagc tgtggtgggg 7140gagaaatcga atgggtgggg gagagagggt ggggggctgc ggtggggttg gggctggagg 7200gggtagggtg ggtgggtgga

ggggtggtgg ttgggtggta gtgggacaac tctcaaacat 7260gattctttct caaacatgat tctttctcat atcctttttt ggatttcctt aaaaaaatcc 7320atatccaaat aacgttgacc ggtgagggac aactctttaa cactattctt tctcaaatct 7380tatgaaatct tcacatttaa ctcgatctcc ctttcaatga acctaaagat atatattaat 7440agagttgcaa attcctaaac tctagaaaat tcaaatacaa cataagagtc ctagattctt 7500ccacaagatg tattatatct ttcaaagttt cccagataaa attagtaatt aggaaactcc 7560ttaacaagga tacttaaagt tttatctaaa tcttgcataa attgaaatcc aaccataatt 7620atgaataaat aatcataaag aatcctaaca taaataacta gaaaataaga taataaagaa 7680gcaacaaaag aatctcataa ccaccatttg aatcccgcat gagaacccaa atagttgttg 7740ttccttataa aaacccacca cctttcttcg ggtattatga cggtattgga ctatagtatg 7800agacgagatc tcttaatcac caatcaacta ttgtaaactt gtgagcctga ataatttatt 7860tgagatacaa ttctaaggtt gtttatgaac gtgtttggta aaattgttat tgataactcg 7920ttcgtggaaa ataaatgcaa aagtcaacat gccaaaaaaa gtgctaaaat caactttcgg 7980ctttgcttga aatgttaagt tttaagctat ccaagagcca ttagtcaaaa tctattgaaa 8040gcgtactcaa aaaccattta tcaaacaccc ctacaaatcc ctttagaaaa caataggagt 8100tgtacaatat aagtattgag ttataaagtt gatcaagtga tttaggaggt tgttccaaat 8160caatctacaa gagtttgtat acttataccc cttcgttttt ttaattgtta cacttaggcc 8220ttgtttgaca aatagagttt agcggttaga gtttagattt tgctgttaga gttttaactt 8280tttgttaaat agatttgact gctgatttga caacttcttc ttataaatgt gtttggtaat 8340tattaacaga ttgctaaaag cttattaccc tttatttatg tgaaatgaca tgtatagaca 8400ttttaatcca catgggtatt attattatta ttattcgagg catacaagtc attgaatata 8460tttttaccta atcctctaaa gaaaaagctc ctattaggag ctttttcatt tcgagagttt 8520ttattccaga actctcttca aaactctttt ttaccaaaga ataggagctt tttcatttca 8580agagttttta ctccagaact ctctttaaaa ctctttttta ccaaacaccc cttttagagt 8640ttttgactag tcaaaactct aaaagtggtc caaatttctc ttttaactcc aaaactctaa 8700ttgccaaaca cccccttaca cttttcacgc ataccaatgc aacactttga cgattaacat 8760ctccagtttt ttatttgtaa aaattataaa gagtgcatat taataagtag ggctgttcaa 8820agtgcggtct ggaccgcacc aaaccgcaac ccaaaccgtt gtttcgcggt ttggtttggt 8880ttgcggtttt aaaattgcgg tttgggttat gatttcaagc aaaccgcggt ttgcggtttg 8940ggttgggttt ttatttttgt aaaccaaaac cgcaccgcaa accgcaatgt tacatttttt 9000ttaaaaaaat aaattaaata catttatgaa ggtgacatac aattataaaa ttgaaaaaag 9060aagtttgagg taaaaaactt taacacttat gataaatcat tatatatgtt taattatgaa 9120ttcagcttca tatctatttg gactcttatt aacaattttc ttttaatctt aggaaacaaa 9180agtaatgtcg cggaggaaat aatggttaaa ccgcaaccca aaccgcacca aaccgttttg 9240cgcggtttgg gttgggttgg tttgggaaaa agtgcggtgc ggtttgggtt ggaaaatttt 9300caaaccgtat atttgcggtt tgggttgggt tacatcccaa accgcacaaa cccaaaccgc 9360gaacacccct attaataaga tacatattaa ttcgaatttg acaagatcca catgactatg 9420tttttattcg cgtataaacc acaaaagaag gttcaagtaa aatttgtgta tggtgtaaca 9480tgtcaatcaa agaacggagg taatatttgt caagacactt tagtcacttc taaattccta 9540taaacaaaga aatatggaag aaaactggtg atgaaaattg aaaaggtggg tataataaga 9600gagacacaat tctaaaataa gaaaatatta ataataaaat aataagttac gataggcctc 9660atgtttgaaa acggaaaaaa taaggagata gttcgtgtaa aaaggaggga gtaaagggta 9720atgcatactt tgtattgcaa gcttagtttt aaaaggcata agacgcaaag cgcatcgagg 9780cacaagacga aggcgcatgc atctcgtagt tgaggcgtgt aatgatttta cttcacaacc 9840acctgagcaa cccaatacag aacgaccaca agaaaaatag aaagaaagga aatgattttg 9900attgatcagc agaaaataca gagcattcga gaggctcagt ctctcccaag gactacaaga 9960tactactaaa tttcacaccc ccttcagtcc ccttacaccc ttatttatac tacttctgct 10020ctcctatttt aacggctact gacattctct gagctggcct gctattcctc tttttgtgct 10080gacatttctg aatattctgt ggtagtggct ccattctcac atttggacag gtttacccct 10140ccatttcttt cgtgcatacg tcagccacgg tttggggatt gaattcatta cattaccctg 10200cccccaaaga ctcaccttgt cctcaaggtg gaaggaaggg aaacgttctt gcaccaaatc 10260tgcatcttcc cacgtggctt caaaaggtgc taagtccttc catttcagta gcacttcagt 10320ctgcgtatgc ctccctctct gtgtctgacg tacgtccaat aattcttcag gttccacaac 10380tagttccaag tctgctgcta gttgagttgg tacagtggtt gctgcctggg catctccaat 10440tgctcgtttg agctgggata cgtgaaatat aggatgtatt ttactggtgc ctggtaactg 10500gagtttatag gcgaccttgc ccaccttttg cagaacaggg aatgggccat agaagcgggc 10560tgccagcttc tcaaatggtc gcttggccaa ggattgttga cggtatggct ggagctttaa 10620gtaaaccaga tcccccactt caaaggactc atcgcgcctt cttgtgtcag cataggcttt 10680catcttttgc tgggagcgta gtagatgaaa gcgtaaatca tcgaggatgg catcccgttc 10740ttgcaacact tcctctaagt tatctactgg cgtttgccct ctgcctactc gccacaagtg 10800tgtgggtcac gcccgtacaa caccctgaat ggagtcagct tagtagacat gtggggagag 10860gtgttgtgtg agtattcagc ccaagggagc cactttgccc aagtcttcgg gtgccccgcc 10920acgaaacatc tcagatatgt ctcaagtcct ttgttcacaa tctcagtttg tccatcggtt 10980tgcgggtgat aggcggtgct tctccttagt gttgtccctt ggagtcgaaa caactctttc 11040caaaaagtac tcagaaaaat tcgatcccta tccgaaacga ttgatgccgg aaacccatga 11100agttttacaa cctccctgat gaaagcttca gccacttgtg agagcactaa aaggatgacg 11160aagcccaatg aaatgcgcat atttcgataa acggtccacc actactaaga tcgtgtccac 11220ccccttggac aagggcaatc cttctatgaa atcaagagtg atatcctccc aaacctgagc 11280tggaatggct aagggctgca gtaggcctgc tggcttttgt tgagagctct tatgctgttg 11340acaaatgcta catcgctgca cgtacaatgc cacgtgcttc ctcataccta tccaatacca 11400ctcagccgcc aacctaaggt acgtttttac ttcacctgca tgtcctcctt ctggggaatc 11460atggtaagct atcatcaact taggaatgat gacggaagtg ttgggaatta ccattcgccc 11520cttataccgc agcttgccat cctccaccgt gaaccccaca agtggtttat ctccctgcgc 11580cacttcttcc ctgagccttt taaggaacca atcctcctct acctcttttt ggagctctgc 11640ccagtccact ccttgggttg tgattatggt ccctagctcc atttcaccta cagtttttct 11700agaaagcgca tccgcaacct tgttggttgc ccccggtttg tagtgtattt caaagtcaaa 11760cccaattaat ttgcttaccc atttctgaaa atcagccccc acttcccgtt gttgtgtgat 11820gaaacgcaaa ctttgttgat ccgtatggat cacaaatctt ctccccaaaa ggtaatgttt 11880ccatttctgg accgcaaggc atatggcaat taattccttc tcataaacgg acttgtgttg 11940tgctctcggt ccaaggagct tgctgtagaa tgcaatgggc ctgccctctt gcattaggac 12000tgcccccacc ccatacccag acgcatccgt ctcaactacg aaaggcttat ggaaatcggg 12060catagcaaga accggtggct gggtcatagc ttcctttaag tgagagaaag ctgaagtagc 12120tttttcggac cagccaaagg agtccttacg caattgctcg gtaaggggct gggcaatttg 12180cgcgtattgc ctgataaact tgcgataata cccggtcagc cctaaaaatc ctcgaagctc 12240cctcaaattc ttgggaactt cccactccac catggccctt atcttctcca tgtctactgc 12300caccccatgc tgcgaaattt acatgcccca agtaggccac tgtcttcctc cccaagtcac 12360atttcttctg gttagcgaac agtttgtgca atgctaacag ctgcaacacc aatcccatgt 12420gtcgtgcgtg gtcctctttg gtcttactgt agaccagaat gtcgtcgaag aagaccagca 12480caaacttcct cagatatgga cggaaaacgt tattcatgag tgactgaaaa gttgctgggg 12540cattggtgag cccaaagggc attacgagaa attcgtaatg tccttcatgg gtgcgaaaag 12600cagtcttatg ggtatcctcc gggcgaacta aaatttgatg gtatccggcc ttaaggtcga 12660gtttagagaa gatggtagcg ccatgtaact cgtctagtag ctcatcaatg accggtatcg 12720gatacttatc cggaaccgtc tccttgttca aagcccgata atcgacacaa aacctccaag 12780aaccatcttt tttcttcacc aataatacgg ggcttgaaaa tggactagtt gagggcttga 12840tgatgcctgc ctccagcatc tctcggatga gtctctcgat ttcgtctttt tgaaattgag 12900ggtaacggta tggcctaacc cccaccggat tactgccttc cttcaacgtg attgcatgct 12960catgccccct ctttggtggc aggcccaccg gagtatcaaa aacttccgca aactgactaa 13020ttaccttctg taaaaattcg ggtacttctt gtgcctcctt caactccgct tctccccttt 13080tcccatcatc ctcaatctgg ttgagctcca agagaaaacc ccctttttct tttcggattg 13140cctttatcat ggctctaagt gagattttag atcttgctaa ggaagggtcc cctctcaatg 13200tcaccactct gccctccact tcaaactgca taacctgagt tttccagttg gtaatcactg 13260accccaattt ctcgagccac tgcactccta atattaaatc tgagttaccc aggccgagag 13320gtaaaaaatc ctctgttact tcgatttccc ccagctttaa agtcacccct tgacacaccc 13380cagtaccatg gacagcttca ccattcccta aagacactcc aaatccccct gcatctgaga 13440tgaccaactc aagttcctca acagttaaca aggaaataaa attgtgagtg gcacccgggt 13500caatcatgac caccacctct cttcctttaa tttttctagt gattttcatc gttttaggac 13560tcatcaaacc aatcacagag ttgagagata cctcagtagg aagttccggt ggtggttcgg 13620acggtggtgc acgagctgcg tcgctcacct cttcggtttc ttcctcctcg tcatgcatca 13680gaatcacgct gatctctttt ctccggcaga tgtggccggc ggtccactta tcgtcacatt 13740tatagcacaa cccttttgct ctcttctctt gatattcttt ctcggaaagt cgcttgaact 13800ctacagattt ttttccttgc cccccagcaa ttggatacgt gttcaaggtg ttggaatttc 13860cccctgggtt ttgggcccac attttgctgg caggtgggtt gaggctggtc gttggattga 13920aggaagcccc tacactcctt gtcattcccc ctctgttata aatcgagtaa ggcccattct 13980tagttggccc acttctttta taacccacaa tcctattcct ttcctcaatt cggcctgcta 14040gttccattgc ttgctctagg tccataggat tgagtaacct gacctccact ttgatatcct 14100cttccagccc attaatgaac tgacccatga gtatttcttc tggtactcta ctcaacggtg 14160ccgccttctc aataaaagtg cgtcgatact catccaccgt ggtggtttgc tttgtggcca 14220accaccgttc ccacaatgaa ccatagtggg ttggtcgaaa ctgacggagg aggtactcct 14280tcagatctgc ccaccacctt atcggccgcc ttttattctc ccactggtac cacctgaggg 14340catccccctc tatagacaca accgccgcct ccagggcttc actgctactc aggccataaa 14400acgaaaaata tcgctcggct ctaaggatcc acccatccgg atcggaccca tggaaaatgg 14460gcatctctaa ctttcgatat ttccagttcc ccccggaagt cgaacctcct ggccctccac 14520ctgagccgcc atccccatac gatcggcccc cgagctggaa acctccatat tcgtcccctt 14580ctcggccccc cagatttatc aggtcaggag ccctccgttc cggcggtcgg gggtgtccag 14640tgacggtttc gggtgtctcc cgcgagggtt gtggcaaggt tgctcggatt tcaacctgaa 14700atttcctctg ttcagcacgc aacccttgaa tcgtcccatc ctgtgtctcc cttgaccggt 14760taatccggtc ctccagccta acggccaaga cctccatctc ctcgcgactc ttcctcgccg 14820cctcattctg gccttccaag atttgagcgg tcaacgattc ccccatggca ttaatggcag 14880attccaccgc cctggacacc atggtggcca ctgacccttc gagggctgcc attctttctt 14940ctaatgaatc caccctctgc acttcgtttc ttggtgccat ggatcgatgc tctgatacca 15000agtgtaatga ttttacttac ttcacaacca cctgagcaac ccaatacaga acgaccacaa 15060gaaaaataga aagaaaggaa atgattttga ttgatcagca gaaaatacag agcattcgag 15120aggctcagtc tctcccaagg actacaagat actactaaat ttcacacccc cttcagtccc 15180cttacaccct tatttatact acttctgctc tcctatttta acggctactg acattctctg 15240agctggcctg ctattcctct ttttgtgctg acatttctga atattctgtg gtagtggctc 15300cattctcaca tttggacagg tttacccctc catttctttc gtgcatacgt cagccacggt 15360ttggggattg aattcattac aaggcgcacc tttggtacca agagacttag acatcaaata 15420aagaagcatt gtaatactta gaataaacat tgtttttata ttctaaaaca catattttct 15480ctaagacacc tagtaatctc atagtggatg tcatctagtt taggtggtaa ggttttattg 15540agttggtact caagacctca gacttagagg tggcaaacgg atcatacggg tcgggtgaaa 15600atgagtcggg tcataatcgg gtcacctttg tgtccaggtt acggtcaggt cgagttcgtt 15660cgggtacgag ttcatattga gtccatgagg tttcatgtca tatcgggtcg ggttagattg 15720gatttacaat ttcgcaaata aataaaacgc atataatact aaagagagta aattaaataa 15780ttaacggaca ctagctaaat catatattag tattttatga tgtattttcc ttaaatttat 15840ttaaaaaata actaatatga caatttttcg ggccgggttc gggttgtggt catcattatc 15900gggtcaattt agtatcgggt aggctcgggt tcatgtcata ttcgggtcta ttttaattcg 15960agtcgggtta tttcggattt aagctctatt tcgggtcagt attttcgatg aagaacgggt 16020ttcggatcgg gtcaccggat acggatctat tttgccgagt cagactgctt ttcaaaccta 16080ataatctcag tttttccacc tattcagatt tgcctatgat ctctatttag ataaatgagt 16140acaatattgt gtctatccat gaaaatgaat atctcacaat gtaaaaggat atctctaaat 16200ttcactaatc actctatctg ttttgaataa taatattcta ttttattgca tgtagtaaag 16260atcgagtatt tagtgagatt tggagaaaag aggaggctag agagaaacta ggatttagag 16320aggagaaggg ggctctgtaa cacatacaag atagatactc ttttacacta acttttcaag 16380atactcaaca tataaaatca gcatcatctt ccaaacaaca actttaagcc acccatgaat 16440cttaattaga taataaaaca taatcgtgaa tcatctatcc tttgtttggg gggatcctaa 16500agcaattgag gaaaagcttt gatgcaaata tcaattgtgt aaaaaagcaa gtattcgttc 16560gtgatgttgc tatactaggt tattttttgg atccaaaagt cattcctact agaatcattt 16620aggaaaattg tcagtatgaa ttttaaattc aggttataac caaagataat tgaaaattgt 16680caaacttttc aaataattcc gaaataaaca tgtttgtaac atggataaac ttttcattgc 16740ttttcaaata attccaaaat aaacatgtta taacatagat aaccttttca gataattcca 16800aaataaacat gttgtatcat ggataaactt ttcattgttt ctagtcactt aaaattctaa 16860aaaaatcttt cctccctact gttactctct ctagcaccaa atctatcaca tgagaaggca 16920gaggttttca aaataaaccg ttacttaatt tggtacttat ttcttgatcg gtgttcatat 16980catatgagtt cctactctat atctctctac tcttctaaat ccttgtgtca cttcctgtgt 17040ttcataaata aaaaggagga agtattagtt ttgaaacgaa aggagtatgg tgcatacatt 17100gatagaaaaa agaagttatt tgtccttatt tcactcatat aacaacacca aattctgtat 17160tgttatcaca aaataaaact tggattatct ttgtttcata gcccaaattt agaattagtt 17220tgtcagattt ccaatcatct aattacaata ttagagctag acctaggaca aaaggtgggt 17280ttggctactt ggtaatagct atgtctagtg ctaggatatg tcattgtcgt agaaccatgt 17340tatggacatc ttaagaaaca aggttaacct aattggttgg agatcctact ttcactttta 17400taataaagtt tcgattcttg cctatttgta aagtagaatt cctaaatttc ccttcactga 17460tatttatctt aacataaaaa aatgttataa acattgggat tgtatataag tcaaaataaa 17520ttgacaatct tggtaacaac taagttaaca ttaattttat aagtaaatga ttaatcccaa 17580tataatctct tatttagtaa atgagacaaa cttgtacacc ttcgtgttag actcgttaat 17640gttcgctaac aattcattca gtagtcaaca gcattttaaa tttgaaataa gtgttcttgt 17700ggtttttgag agatcaagca agaaaacatg tctctcccct ttgaccaact aattgggatt 17760aagaatacta gttttaagat tttaagaatg agttatagtc tttcttagac cgctacaatc 17820cccttgttga tatgaaccag atatattttg tgttcaaata gtagatcaat gcattgttga 17880taatcctttg ttaatgtact tgttgatctt attttgtact tttggtagat gcgctatact 17940ttctttcgat tgctcatttt gaactcttaa ctacatatgt tagtttaagt agatgattta 18000gaattgctat ttcaatcttc aataagcaat ttaagttgtc aaaccttgtt tcacatcatt 18060agggtgaaag ttatttggat aaagacctat atctaattca atccaaagca aattagtaat 18120gcggattgga ctcaaactat gtttagattg gattcgaatt gagtttcttt tctttttctt 18180aaaaaaattg gattttccga tcgaattgag ggtgattaga tccaaaataa ccgaatagta 18240gataggattt gtgttgtata ttagaattgg gcttaaggat ttccatttta acaaaaaaac 18300caaatggtcc gactatcaaa aactataatt tgatagtcat gcctatcgaa aactttattt 18360tcattctcgc acctaattat gggcttgtat aaattagttc tactatcgaa aactaatttt 18420gatgctcgtg cctaatttaa attttcgaaa aaatgaagtt aagaaaattg gatatttcgg 18480attggatcca atatatcttg tgaattatta atttggatta gtttggactc aaattcttat 18540tggattggat tcaaattaaa agattaaaat tcaaattttg ttcgaatcaa attggagtag 18600gcttaagttt aaatcataca ccgaactttc accactaccc atcatgctta agcttctaat 18660gtaagagagt gtttgggagt tgagctcgaa caactaaatt tctaaaagaa ccaagttcaa 18720acaagaaatc taaaagctcg attaaacttg agtcaagctc aaacacctat attccttatt 18780ggagcttgac tgaagattga acacttattc cttattaagc tttacgctaa aacattgctc 18840gactcaactc ttctacatcc ctatagttca aaagaaatag ttgtgggctg tggtgctctt 18900gtagaccaac gcactagttt aacaaagcta agtgcctgac tgcaattcca tacacattac 18960gatcaccatg acctagtttc agctcacact ttggaagtct aatttgaact tgttctctac 19020ctccaattca ttgtggggta ggaggcgata gttaagggat caaaatctta tgatataact 19080tgcataggct atgccactat ataatgcgtc ttgtgtccca tattagttta atcaaattga 19140aatgttttac catttatatc ttcaattatg tatggatact aatatttgat ttgacgtttg 19200atatgatatt aaatgtggac tgttattctt gatgtgcttg agaagctttt ttggggccag 19260ttagaaacta tattcctttt atggtcctaa ctaggttgtt gttggtgtgt tcccaaataa 19320cagcatggaa aggatactcg agcgctatga aagacactca tatgcagaga gacaactgac 19380tgctccagat cctggatccc atgtaatcca gctaggcaac tatcttttct aagcatttaa 19440atcgttgaga tttcaatttt aaatgtgttt taactgataa ttcatgcatt atatgcttag 19500gtaagtttga ctctggaaca cgcaaaactt aaggctaggc tggacattct tcagaaaaat 19560caaaggtaat aagatccaga ccaaatataa tttgtataat aaccacctta tgaggaaaat 19620ttaagatcct tgataatttc aggcattaca tgggagaaga acttgatacc ttgagtctca 19680aggagcttca gaatttagag catcaaattg acagtgctct taaacacatc aggtcaaaga 19740aggtagtttc acagttgcat tagatcatct tatggatcaa ttggatcact tgtttgtatt 19800ttagcgttgc tcaacacggt cgtctaatat agtgtgcaaa acgacctaca gggcaacacc 19860ttttataggg ctcgaaaata cgaaaaatta aatgtttgtt ttagtcatat tgttcaaacc 19920caagctttat cttgtcaaaa atattttata atgattattt tttagaatac attatttaca 19980tttttgcaat ttatgcataa tacttctaag gtccaacttt ataattgaaa tagaagtcct 20040taaattttaa agacgacctt gaggaaacct aatttcttct catatataat taaatcaatt 20100attctacaag ttagtagaac aaatactaca ataacaacaa tattgaagcc ctaatctcag 20160taggattgga ttgattgtat gaagtcttat tagtggccgt taaatgtttc ttgtaggtca 20220agatgacatg gctcatatag taaggttact tgactaaaag acgaggattt gtttcgactt 20280agattttaac aagtttccct catttgttaa cacctaagcg tactaaatca aattctaggt 20340tttactcact caaatttccg atttaggaag ggcttgagga tagttgtatt atcgtaactg 20400actaatcaaa ggagcctctc ttagatcagg tttcacttgc caattctaac aacttgtttg 20460gtaaaaggaa tttggaatga aaagaaagga attgaaaaga aacattctac ttttcaatgt 20520ttcattcaaa aataacattt taagtgatag gaaatggaaa gaagtgaaac gaaagcctct 20580ttacaaaatt atcatttttc tacccccccc cccccccaaa aaaaaaaaaa aaaataagta 20640gtaagtagta gaagaagaaa taaataacta acaagagtag taagttttta cgttttcttt 20700ctnnnnnnnn nnnnnnnnnn nnattcaaat cagaactgaa tagtcataac cggaagatta 20760gtttctctct agcgtgacta gggtttgagt aaaaagagaa aacttaaatc aaacatggga 20820tattaaggtt ttttttcctt tcttcagttc ttttctcttc ccaatccttt cctaaaaatg 20880aaccaaacag gctgcaaggt tttcacttgc ttaacacaag atttattttt aaaaataatt 20940acactccaaa cttttaagct taaaaccaat tttaattcaa atcagaactg aatagtcata 21000actggaagat tagtttctct ctagcgtgac tagggtttga gtgaaaaagt ctagggtttc 21060atgtcattct tcttgcttcg agtcccttct tgggattgtt gttagccatt atggctaccg 21120aaatcgttat taaatgtcta aatcttagaa ttactgctga agaaaacaac ttggtgtttc 21180tcgaagatgt tgatgataac tcgcagcacc atacgctcgc actggcgatt gttggaaagg 21240ttctttcgtc aagaccatac aatttcgagg cacttaaatg aaccttaaac tagatatggg 21300tgatatccaa aggagcccta cttcacccta ttgaaaacgg actttttgtg gtacaatttg 21360cgacaattaa ggaccgatct aaggttctag tcagcagacc atggaccttc gatataaacc 21420ttgttctctt agatgctatt gaagggggta ctcaatcttg acccattgcc cgttttggac 21480tcgcttgtat aaccttccta tggactgccg atatgagaag ttcatcaaaa actattgttg 21540gtgtattggg ggaggtattg gaagttgatt ttgacaggat tgtttgggat aaatctgcaa 21600gagtaaaggt gaagattgac attacaaaat cgttttgtcg tgtgcagatg atcaagacta 21660acaggggtga ggctgtgatg atcaatgtta agtatgaaag acttcctaca atttgttatg 21720tgtggaattc tggccatatt gaaagagatt gtgtgaagac ccaggaagaa gagaaacaag 21780tggagagaca atagggggtc ttggaggcct ctccgcgtag gggacgatta aagatggtga 21840aagagtcgaa agccttcctt cagtgtgctc gtacactcca ctttaataac aaggaagaag 21900taaggggtga ggaaccacgg gattatgtgg agccgagggg ttattgtcgg ctatcttagg 21960gggtaaaact ttggtggtcc aggagatagt ggacggctct aaggatgcca tcgaggaagt 22020tcgtgctgaa ggtgcaccac tctagccccc ttgtaccctt tgggtaatgc catgctacct 22080tttacttttg ctgttgggag tgctaatcct actccctccc accgaaaagt taaaattaaa 22140aacaaggcaa gggttcaggg tgttttgaac caagttaatg ttgtgggtgt tggggggttg 22200gctaataatg ggggttgtga gaaaaggata ttccccaacc cgatggtgtt agaaaaagaa 22260aaggggttca atgaagaggg

tttaagatag caaaacgaga ggattgtatg taacctatca 22320gtagggaggt aactattgag gtggaggtgg gcgagaccca accccgcccg acattatgaa 22380tatcctatgt tgcaactgtt ggggattggg caacccccgg gaagttcgga tgcttcgtag 22440gtggagcaat agtgctacac tgagttcggt ttttatttct aaaactatga ttagtggtcg 22500tgatgtggaa agggtgcaaa gcgggtaggg ttttgattgg gcaattgggg tggatagcgt 22560tggaacttca agagtttggt gcatttattg gaaagctggg gaagtggact ttactctagt 22620ctctctatca agtcatcata tttgtgggaa tgtgaagctt gttgatggga aggtatgatg 22680cttagtgagt atttatggtt gggcggatac aattcaaaag tataaaacat gggagcttat 22740gcaatccttt cactcatatc atgggccgat attgtttggt tgggacttca atgagatttt 22800gacaatcgga gaaattgaag gagggtccga aactcaatga agtaacatgc ataattttct 22860agaaacttta gatgacatga agcttaggga ccttggctat tcgggaactt ggtatacata 22920agagagaggc tttaagccac ggaagagaat gagggagaaa cttgatcatt ttgttgcatc 22980ttcatcatgg tgtgacttct ttccgaaagc tacagttgag cacttgatgc gctacaaatc 23040ggaccacact cctattttgg ttcgccttgt aggccatcag tgaagacata agaagaaaaa 23100gacgtagttt tgttttgaga ctgcttgggt gcttgaggaa ggttgtgagg cccaatgggt 23160gagtcatggg ccgggtttac tcgcgaggta tttatcgagc gctttaaagc cgtggaaggt 23220gggttcaaag caaggagtga tgggtctctt agtaatctgg gcccgcgtgt gagggagatt 23280gaggaggcca ttatagatgg gaggcagcga agcagataag gactatgagg ctctatgaga 23340ctcctctccc acgaaagtta gacgaggtgt tggacaagca ggagacgttt tggtttttga 23400ggtctcgtgt gagttagata aaggatggtg atcgtaatac acaatatttc caccacaaag 23460cttcccaaca caaacgtcgc aactacatag cggggatgta tgataataaa ggggtgtggc 23520aagataacga agaggatatt gaagggaata tttcagagta ttaccaaacc tcgttcggtt 23580cgtgctcccc ctctaggaag aacgtcgcgg ttgtccttga ggttgtgagc ccggtgataa 23640ctgatgatat gaatatggcg gttatgaaat cttacactaa agatgaggtg tgggaagcac 23700taaaccacat gaagcctaac ggaatgcatg ccatccttta tagaggttct ggaatacctt 23760ggagatgata ttacatctgt cattttaggt attattcatg gcacccgacc ccagatgttt 23820ttaacaagac taatattgtg ctcattccta aagtcaaatc cccaaatctt gtttctgagt 23880ttcgcccgat tagcctctgt gatgttatct ataaacttgc ctcaaaagta cttgctaaca 23940gattaaaaca ggtttgcctg acattgttta tgataaccag agtgcatttg tgtccggaag 24000ctatattacg aacaatgctt tgatttctct tgaattattt gactctatga aaaaatgata 24060cagagctagg aaaggttttg tgtcgatgaa attggatatg agtaaagcct ataaaagagt 24120tgagtggtgt tttttcagta gtgtgttgga gaagttggat tttgctgaat catgagtgaa 24180tgttgttatg agatgtgtgt cttttgtgca gtactctttt gtggttaatg ataatatatg 24240tggagctctg acaccctcaa gggggctttg acagggagac cctatatccc cgtatttgtt 24300tatacttgtt gcagataccg ttttagctct tcttagcaag gcattcaaca atgcgtggct 24360atacttgata ttctcaacaa atatgaggca gcatcaggct agaaaataaa tattgacaag 24420tcaggaatct ctttcaataa aagatttgac gtattttatg gccatgaaac aagttgagaa 24480gcatcagaaa gacttggtat cccaactttg gctaggagtt cgaaaaaagt catatttgct 24540gacattcaag agcgaatttg gaagaagctg cacggatgga gagaaaaact tctcgcgggc 24600ttgaaaagaa actctcttaa aagttgtggt tcaagcaatt ctaacctatt tggtgggcgt 24660ttacagattc ctaaccagta ttatccaggc cattcatttg gccatggtaa agttttggtg 24720ggggtcgaaa agggcccaca attcgatgca tctgggggga tatgtgctca ccaaaatgtt 24780taaggagcct tagctttaaa gacttagggg tgttcaatga acctaaacta aggaggaatg 24840cgtggcattt gattcctgct ggtgagtccc tttcgggtcg agtgttctcg gccaagtact 24900attcgaagtc aacctttttg gactcatttc taggtccggt aggtagcttc tcttggaaga 24960gtatttgggg ggccaaggca ttagttaagg gtgttttatg gtgcgtaggc aatggcagac 25020aaatcaacat atggcgtgac tcgcgggtgt tgaatggtga tagtaggttc atccccggag 25080agcgcgtttc aggccttgag gatgtttgtg atctaataga ttttgcacaa tggagtgcga 25140tgtggacctt gtcacgattg cttcaatgaa gatgatgctc aagccatttt agtcatacct 25200ctaagtaagc gccttctgaa ggacatggtc tcttgggctt tcactaagga tgaatttttt 25260ttgtaaaaac aacctatatg gccggttggt cgaggaattt gaatttgttt cacaaagcat 25320ggctgcaaat ctggggcctt aacgtgtctc cgaaggtctg ccacttcctt tggcgtttat 25380gctcggtacc cttcctgttc gagctctttt aaaacgacgc cacataactg atgatgattc 25440atgtcctttg tctaaaggag cccggaaagc atatcacacg cgttgttcta ttgcccatat 25500gtagccgaag catgggagag tgcgggcctc acaaattgtt tgcctttgtt tgatggggct 25560ggtatgcttg atgcgtgggg ggagtgggaa acaatcgatg actagtccct tgtaagactt 25620agcttcttgg cttatcactt gtggtttagg cgaaataaat gtgtttttga aggggtggtg 25680agagcgaatg agagtgttgt ggaatatgcc actaaagcta ctgttgatca tggtttgtat 25740agtgcccgca tttatggtgg gtcgaaggct accgcatcca aaagctcgaa ggtatgggtt 25800ccccctccag cttgtcgtac gatctaaagg ttgatgcatc agtggggaat ggtggatggg 25860tggggctagg agtaatcgcc tgaaactaga aaggggaggt gctcgtggct gcaactagga 25920gggtcagagc ttgtggcccg tggaaatggc tgaagggaag gctctttgtc ttgctcttag 25980gcttgcctcg ctcatacaac ttgcaagaag tgatcgtgga gtttgactgt caatcttggt 26040gaaccatctc tccaagggtg ctatttactt tgcattttta agtcaaagct tgaaccttga 26100taaaaaaatc ccgttcgaca tgaaaagtgc cttgattttg cgggtttggg agtgccttat 26160tgattctggg gtttgatttg taacaccttt agtaaaaaca tgtaagctaa ctgtaaaacg 26220aacattaatc aaactaggat atgtaaaatt cctaaatcaa gaagaatttc cacttgtgct 26280gaatttgtcc accttgcatg acacccaata aaagcccatg tctcctagaa ccccttatgc 26340cgccttattc atcttttctc aagttgagtt ggagtcctct atggtccact cgacttcttt 26400agcacactct cggtaaaaac ttttaatatt attttatttt agactccacc atcttgacat 26460ttattccttc ttaaacttgc ttcacacaaa catctaacac tagaattcta tatagaatag 26520cttgaatctc tcttaggata accttatagt aaatgcaact acgcctatcc ttaaaccttt 26580ctaagaggag ctttatcgta tttacattcg cttcactttg aaacgtcgct aagtgtatgt 26640tgcactttcc aaaccatgtg ttagctaaga ccaagttata tgactgcata acctaaatag 26700tcttcctcga gaaaattcac tagttggatc ggaagagttt gtgtaaatct atggcggcgc 26760gggactgggc cttcacgatt tcaagtgttt taatgcagct cttctaggta aacaagtttc 26820ctaatagcgt ggtgactcaa atattgagga cttgttgtta tactaatgct attcctggcg 26880gcacttaagg ggtgaaatag gtggtacaaa ggagtgcttg atggcgtgtg ggtagtagtt 26940gaatatatca gtatgatcaa gtccatggat ccctcgtact tattcgtgca agattatttt 27000tccacgaggc aaagcgagcg agaatcttaa ggtttatgat catattcatc ttgtacgtgc 27060taagggtaat gtccctttca ataatgagct atttctcctt ttgagcaaga gcgtatctta 27120agcattcctc gtagttctcg tctccccaac gatgttttat gttggaatct gaatttggag 27180aaagacggag acttttcgtt cggtctatcg agccattctt ttgagttgga tggcgagagc 27240gtgatttcat cgtcaatacg ctctaattta tggagtataa tatggcagga tagtaccttt 27300caacgtgtta agcttttatc tgcattgtcg acacaaaggg gattgagtaa gcctgtgccg 27360agtatggaac cattgtgtaa tctttatgcg ttggaggatc aatggagcta cacttcttac 27420gagactttgt tattggaagg gcttatatgg gatccaacta gggtagtcaa aacattggtc 27480ggggctgcgc tgcaattttg gggacttggc accggcgttt ttggagtagc tccctcatgt 27540ggaacatagg cttttgatga cgatatacta ggctagatgg aatataagag agaggtgttt 27600gtttgaggag gaggtttgtg atccctatca aaccacatgc ataatctcat ggcgtagctt 27660catgtggaac actgacatat tgcatgtgta gggttaggtg cttgcctagg acaccacatg 27720ggaagagctc cgttaagctt aatgtggatg gagggtgtgt ggaagggttg ggtgcgtcca 27780ctggagtggt gattaggggg atggatgaaa aagcgttgta gttgcaacat agaaaggtgg 27840aggactgcga ggaaccgtta aaaaggctat attttatggt gttcatttgg ttgtggaagt 27900cgatttttga aatatggttg ttggaagtga ctatcttcac ctcgttgaag caacttcttc 27960aaaagtggaa ggcaaaaata gcttccatgt tattgttgat gacattgttc atggtagtgg 28020tatgttaaat acttcgtctt gtagttttgt tcgtagggat gggaataggg tttctcacga 28080actcccccat ggaaatatca taatggctca tgctcatagg taaatgaata agctaacaaa 28140attgttcatc tttgcagaac caactcatgc atgaatcgat ttctcagctt cagcgaaagg 28200tagacagctc tagagagagc atctagtctc aaaaatacca tgagattctg tagtgtcctg 28260acatgtttta tatgacagga caaagcgtta aaggagcaca acaacttgct atccaagaag 28320gtataagttc agcaagattg tttagtaaca ttgttaatct tgctgattgc tttgaaacat 28380gtcttgctat ggttaacaat gttgactgaa ccaaaatagg tgaaggagag ggagaaggtg 28440ctggctcagc aggcagaatt ggatcagcaa aatcatgaca ataactcatc tggctttgtg 28500atgtctcaag ctttgccctc actgaataca gggtcagtcc tcaataacct ctaatcattt 28560ttccaagatc caaagtaaac atggtttcat aatttaatta agattttttt gaaccatgtc 28620tccatacaac cttactagga ctaatactac taatttaaga ccccaacgat aaacaacaat 28680aattagccat atctggctag caccttttgg acaacacacc acatgagact cttggccaac 28740ttctttgatt tccttcagtc tgatagatat gaatatcttc tgaagagctc tttggttcat 28800aattattgat ttagaaaaga attcagcaag gtgagtcatt tggtaacctt aaggtcatta 28860tgggggtact aaatcaaagt gaagatatat ttaggtggca tcagaagaga tgatatagat 28920aggttgtatc ctgtcgatag gttatttgga tatgtatcaa aagtttcttt tataatatat 28980ctatactgat tggttgatgt atcaaatatc cctacagatt gtgaaaaaat cccctacaga 29040ttgtgaaaat atccctagaa cctgtgatga tataagatgt gctccgcatg ctttattgaa 29100cataatgtat tcaattcttg aaatgcagag gaacaagcag cagtgcagtg gaagatgaag 29160caacacaacc accaaatcta aacagcaact ctgcacaaat accgtcctgg atgcttcaac 29220acatccaaga gcagtaa 292374735DNAArtificial SequenceBvFUL cDNA 4atggggagag gtagggttca gctcaaaaga attgaaaaca agatcaaccg tcaagtgacc 60ttctccaaac gtcggattgg attgttgaag aaagcgcacg agatctccat tctctgcgat 120gccgatgtag ctctcatcat cttctccact aaaggcaagc tcttcgagta tgcttctgat 180acctgcatgg aaaggatact cgagcgctat gaaagacact catatgcaga gagacaactg 240actgctccag atcctggatc ccatgtaagt ttgactctgg aacacgcaaa acttaaggct 300aggctggaca ttcttcagaa aaatcaaagg cattacatgg gagaagaact tgataccttg 360agtctcaagg agcttcagaa tttagagcat caaattgaca gtgctcttaa acacatcagg 420tcaaagaaga accaactcat gcatgaatcg atttctcagc ttcagcgaaa ggacaaagcg 480ttaaaggagc acaacaactt gctatccaag aaggtgaagg agagggagaa ggtgctggct 540cagcaggcag aattggatca gcaaaatcat gacaataact catctggctt tgtgatgtct 600caagctttgc cctcactgaa tacaggagga acaagcagca gtgcagtgga agatgaagca 660acacaaccac caaatctaaa cagcaactct gcacaaatac cgtcctggat gcttcaacac 720atccaagagc agtaa 7355259PRTBeta vulgaris 5Met Gly Arg Gly Arg Val Glu Leu Lys Arg Ile Glu Asn Lys Ile Asn1 5 10 15Arg Gln Val Thr Phe Ser Lys Arg Arg Ser Gly Leu Val Lys Lys Ala 20 25 30His Glu Ile Ser Val Leu Cys Asp Ala Glu Val Ala Leu Ile Ile Phe 35 40 45Ser His Arg Gly Lys Leu Phe Glu Tyr Ser Ser Asp Ser Ser Met Glu 50 55 60Lys Ile Leu Glu Arg Tyr Glu Arg Tyr Ser Tyr Ala Glu Arg Arg Leu65 70 75 80Ala Ser Asn Asp Pro Asp Ser Gln Val Asn Trp Thr Phe Asp Phe Ala 85 90 95Lys Leu Lys Ala Lys Leu Glu Leu Leu Gln Arg Asn His Arg His Tyr 100 105 110Leu Gly Gln Glu Leu Asp Ser Leu Asn Met Lys Glu Leu Gln Ser Leu 115 120 125Glu Gln Gln Leu Asp Thr Ala Leu Lys Asn Val Arg Ser Arg Lys Asn 130 135 140Gln Leu Met His Glu Ser Ile Ser Glu Leu Gln Lys Lys Glu Arg Ala145 150 155 160Met Gln Glu His Asn Asn Ile Leu Ser Lys Lys Ile Lys Glu Arg Gly 165 170 175Lys Asn Leu Glu Gln Val Gln Gln Met Gln Trp Gln Asn Gln His Gln 180 185 190His Gln His Gln Gln Gln Pro Pro Pro Pro Pro Gln Asn His Gln Val 195 200 205Pro Pro Asp Ala Ser Asn Phe Met Leu Pro Pro Pro Ile Pro Ser Leu 210 215 220Asn Thr Gly Gly Tyr Gln Gly Gln Phe Gly Gly Glu Val Arg Arg Asn225 230 235 240Asp Leu Asp Leu Thr Leu Glu Pro Ile Tyr Ser Cys His Met Gly Cys 245 250 255Phe Thr Thr6244PRTBeta vulgaris 6Met Gly Arg Gly Arg Val Gln Leu Lys Arg Ile Glu Asn Lys Ile Asn1 5 10 15Arg Gln Val Thr Phe Ser Lys Arg Arg Ile Gly Leu Leu Lys Lys Ala 20 25 30His Glu Ile Ser Ile Leu Cys Asp Ala Asp Val Ala Leu Ile Ile Phe 35 40 45Ser Thr Lys Gly Lys Leu Phe Glu Tyr Ala Ser Asp Thr Cys Met Glu 50 55 60Arg Ile Leu Glu Arg Tyr Glu Arg His Ser Tyr Ala Glu Arg Gln Leu65 70 75 80Thr Ala Pro Asp Pro Gly Ser His Val Ser Leu Thr Leu Glu His Ala 85 90 95Lys Leu Lys Ala Arg Leu Asp Ile Leu Gln Lys Asn Gln Arg His Tyr 100 105 110Met Gly Glu Glu Leu Asp Thr Leu Ser Leu Lys Glu Leu Gln Asn Leu 115 120 125Glu His Gln Ile Asp Ser Ala Leu Lys His Ile Arg Ser Lys Lys Asn 130 135 140Gln Leu Met His Glu Ser Ile Ser Gln Leu Gln Arg Lys Asp Lys Ala145 150 155 160Leu Lys Glu His Asn Asn Leu Leu Ser Lys Lys Val Lys Glu Arg Glu 165 170 175Lys Val Leu Ala Gln Gln Ala Glu Leu Asp Gln Gln Asn His Asp Asn 180 185 190Asn Ser Ser Gly Phe Val Met Ser Gln Ala Leu Pro Ser Leu Asn Thr 195 200 205Gly Gly Thr Ser Ser Ser Ala Val Glu Asp Glu Ala Thr Gln Pro Pro 210 215 220Asn Leu Asn Ser Asn Ser Ala Gln Ile Pro Ser Trp Met Leu Gln His225 230 235 240Ile Gln Glu Gln72366DNABeta vulgaris 7atgatagaac cgcagctgaa agcatgcaac aaaaatgtga agaatccgga gagcaggaag 60actgcttcca cttcgtacaa ttctgcttct aggaagcaaa gcaggaaggg agaaaatcct 120attcgtgtta ctccgttagg agagcaatct tctgattttg gatgttctag tacttggata 180tgtaaaaatt ctgcatgtag agctgttctg tctatagatg atgcgttctg tcggaggtgt 240tcatgctgca tctgtcatca atttgatgat aataaagacc ctagtctttg gttggtttgt 300gaatccgagt ctgggcaggg tgattcttgt ggattatcat gccatattga gtgtgcattt 360caacaagaaa agctgggagt tgtgaacctt gggcaataca tgcatttgga tgggagttac 420tgttgttctt cttgcggcaa agtctctggg atacttgggt cagtacttct gttttatgta 480gtgagatatt gacctgaagt catgcttgtt ttgatggaag ataaataatt taaaaaaaat 540gttacatgcc caactattac aaggccagta gaaaactatg agatatatta attttgatat 600tactgtggag gctcagttga atttatatgc ttgagtttta ctcactaatg gcaggtgttg 660gaaaaagcaa ttggctatag ctaaggatgc tcgacgtgtc gatgtgcttt gctatagaat 720atttttgagt tacagactcc tcgagggcac agctaagttt aaggacctcc acgagattgt 780tgcagaagct aaaacaaagc tggaggcaga ggtgggtcct atgaacggag actctgtcaa 840gatggccaga ggtattgtta gcaggcttgc tattgctgca gatgtgcaaa agctctgttc 900gcacgcgatt gataaagcta atgaatggct cgccaatgtt tctagcatta gttcaaattg 960caaaggttag aatacataca gcctttattg ttctccattt acttggtgag tattctatta 1020taataaatta ttaatttctt ttggcataat ggtggcagtg gatgcacttc ctgctgcatg 1080caggtttcta tttgaagaag ttacttcttg ttcacttgct atagttttga tagatatccc 1140cacaccaatg actgattccg tcaaaggcta caagctatgg tactgcaaaa gtagacatga 1200gacttttgca agggagccta catccgtctt tccaagggag aaaagaaaaa tatctgtaaa 1260gaatctcaag ccttgcaccg agtacacatt cagaatagtt tcctacacag aagttggtga 1320tttaggccac tctgaggcta agtgtttcac caagagtttg gagatcatta gtaagaaatc 1380caccacagtg ggctgtaaga aggaagatcc ttgtgttgag aggagctcct cgaatgcaaa 1440ggaacaacat aattcaaatt tggctgcaat atcttctgga ttcaaggtgc gggaccttgg 1500gaaaatcttg cacctagcat gggcccaaga acagggttgc cttgaaggtt tctgcagtgc 1560tgatgtagaa caatgctgtg gagtaactaa atgtgaatct ccaaaagatc accagtcacc 1620tccacctgtt tctcgtgagc ttgacctaaa tgtagtttca gttcctgatt taaatgaaga 1680ccttacccct cccttagagt cttcaaggga tgaagacaac ggatgcacgc tagagcgtgc 1740tactgggcct gatgatgatg ctgcttccca tggtgttgag aagaatgggc ttgggctagc 1800caggtcaaat ggtagtgggc caagtgatga gtctcaagct tgggctctca tccgaaatgg 1860agatgtgcct gctgttgatt ccttggcaga gacccgtcgg aagaggtctt caagtgcgaa 1920tgaagaaaca catgactgtg acagcactct gataaatgga tcgccatttc ggatttcagg 1980cgggcctggt tctctagacg gtaactttga gtattgtgtg aaggtcatcc ggtggttgga 2040gtgtgagggc tatctaaaac aggaatttag attgaaatta ttgacttggt ttagcttaag 2100atctactgaa caagagcgtc gggtagtcag cactttcatt caaactctga tggatgatcc 2160aaagagctta gcaggacagc tagttgattc ctttggagat ctcatatcca gcaagaggcc 2220caggactagt ttcactagta agtttatgtc tatagttgtt ctttgattga gaaattttat 2280gtcttctttc aaacatttct attactcttc ttattctgaa gttttggact aattttgtca 2340tgctaattac aggcattcct tcctaa 236682285DNAArtificial SequenceBvVIL1 cDNA 8atgatagaac cgcagctgaa agcatgcaac aaaaatgtga agaatccgga gagcaggaag 60actgcttcca cttcgtacaa ttctgcttct aggaagcaaa gcaggaaggg agaaaatcct 120attcgtgtta ctccgttagg agagcaatct tctgattttg gatgttctag tacttggata 180tgtaaaaatt ctgcatgtag agctgttctg tctatagatg atgcgttctg tcggaggtgt 240tcatgctgca tctgtcatca atttgatgat aataaagacc ctagtctttg gttggtttgt 300gaatccgagt ctgggcaggg tgattcttgt ggattatcat gccatattga gtgtgcattt 360caacaagaaa agctgggagt tgtgaacctt gggcaataca tgcatttgga tgggagttac 420tgttgttctt cttgcggcaa agtctctggg atacttgggt gttggaaaaa gcaattggct 480atagctaagg atgctcgacg tgtcgatgtg ctttgctata gaatattttt gagttacaga 540ctcctcgagg gcacagctaa gtttaaggac ctccacgaga ttgttgcaga agctaaaaca 600aagctggagg cagaggtggg tcctatgaac ggagactctg tcaagatggc cagaggtatt 660gttagcaggc ttgctattgc tgcagatgtg caaaagctct gttcgcacgc gattgataaa 720gctaatgaat ggctcgccaa tgtttctagc attagttcaa attgcaaagt ggatgcactt 780cctgctgcat gcaggtttct atttgaagaa gttacttctt gttcacttgc tatagttttg 840atagatatcc ccacaccaat gactgattcc gtcaaaggct acaagctatg gtactgcaaa 900agtagacatg agacttttgc aagggagcct acatccgtct ttccaaggga gaaaagaaaa 960atatctgtaa agaatctcaa gccttgcacc gagtacacat tcagaatagt ttcctacaca 1020gaagttggtg atttaggcca ctctgaggct aagtgtttca ccaagagttt ggagatcatt 1080agtaagaaat ccaccacagt gggctgtaag aaggaagatc cttgtgttga gaggagctcc 1140tcgaatgcaa aggaacaaca taattcaaat ttggctgcaa tatcttctgg attcaaggtg 1200cgggaccttg ggaaaatctt gcacctagca tgggcccaag aacagggttg ccttgaaggt 1260ttctgcagtg ctgatgtaga acaatgctgt ggagtaacta aatgtgaatc tccaaaagat 1320caccagtcac ctccacctgt ttctcgtgag cttgacctaa atgtagtttc agttcctgat 1380ttaaatgaag accttacccc tcccttagag tcttcaaggg atgaagacaa cggatgcacg 1440ctagagcgtg ctactgggcc tgatgatgat gctgcttccc atggtgttga gaagaatggg 1500cttgggctag ccaggtcaaa tggtagtggg ccaagtgatg agtctcaagc ttgggctctc 1560atccgaaatg gagatgtgcc tgctgttgat tccttggcag agacccgtcg gaagaggtct 1620tcaagtgcga atgaagaaac acatgactgt gacagcactc tgataaatgg atcgccattt 1680cggatttcag

gcgggcctgg ttctctagac ggtaactttg agtattgtgt gaaggtcatc 1740cggtggttgg agtgtgaggg ctatctaaaa caggaattta gattgaaatt attgacttgg 1800tttagcttaa gatctactga acaagagcgt cgggtagtca gcactttcat tcaaactctg 1860atggatgatc caaagagctt agcaggacag ctagttgatt cctttggaga tctcatatcc 1920agcaagaggc ccaggactag tttcactagc attccttcct aaataaatct taactaagga 1980cggcacacat atcttggata caattcagat gtttaggaca caatttttag gaggcagtac 2040ctgattttcc tcgagaaagg gattccatca gtggttaact gcacatttta gaaggtattt 2100gttagagttt ccttgaccac atttgtagaa agattcacat tgagacaatc attgttgcct 2160tctcgcattg aaggaaggat atatgcttca atgaatattt aaattctagt tcaatttact 2220aattaattag tttgttttct caaaaaaaaa aaaaaaaaaa aaaaagtact agtcgacgcg 2280tggcc 22859653PRTBeta vulgaris 9Met Ile Glu Pro Gln Leu Lys Ala Cys Asn Lys Asn Val Lys Asn Pro1 5 10 15Glu Ser Arg Lys Thr Ala Ser Thr Ser Tyr Asn Ser Ala Ser Arg Lys 20 25 30Gln Ser Arg Lys Gly Glu Asn Pro Ile Arg Val Thr Pro Leu Gly Glu 35 40 45Gln Ser Ser Asp Phe Gly Cys Ser Ser Thr Trp Ile Cys Lys Asn Ser 50 55 60Ala Cys Arg Ala Val Leu Ser Ile Asp Asp Ala Phe Cys Arg Arg Cys65 70 75 80Ser Cys Cys Ile Cys His Gln Phe Asp Asp Asn Lys Asp Pro Ser Leu 85 90 95Trp Leu Val Cys Glu Ser Glu Ser Gly Gln Gly Asp Ser Cys Gly Leu 100 105 110Ser Cys His Ile Glu Cys Ala Phe Gln Gln Glu Lys Leu Gly Val Val 115 120 125Asn Leu Gly Gln Tyr Met His Leu Asp Gly Ser Tyr Cys Cys Ser Ser 130 135 140Cys Gly Lys Val Ser Gly Ile Leu Gly Cys Trp Lys Lys Gln Leu Ala145 150 155 160Ile Ala Lys Asp Ala Arg Arg Val Asp Val Leu Cys Tyr Arg Ile Phe 165 170 175Leu Ser Tyr Arg Leu Leu Glu Gly Thr Ala Lys Phe Lys Asp Leu His 180 185 190Glu Ile Val Ala Glu Ala Lys Thr Lys Leu Glu Ala Glu Val Gly Pro 195 200 205Met Asn Gly Asp Ser Val Lys Met Ala Arg Gly Ile Val Ser Arg Leu 210 215 220Ala Ile Ala Ala Asp Val Gln Lys Leu Cys Ser His Ala Ile Asp Lys225 230 235 240Ala Asn Glu Trp Leu Ala Asn Val Ser Ser Ile Ser Ser Asn Cys Lys 245 250 255Val Asp Ala Leu Pro Ala Ala Cys Arg Phe Leu Phe Glu Glu Val Thr 260 265 270Ser Cys Ser Leu Ala Ile Val Leu Ile Asp Ile Pro Thr Pro Met Thr 275 280 285Asp Ser Val Lys Gly Tyr Lys Leu Trp Tyr Cys Lys Ser Arg His Glu 290 295 300Thr Phe Ala Arg Glu Pro Thr Ser Val Phe Pro Arg Glu Lys Arg Lys305 310 315 320Ile Ser Val Lys Asn Leu Lys Pro Cys Thr Glu Tyr Thr Phe Arg Ile 325 330 335Val Ser Tyr Thr Glu Val Gly Asp Leu Gly His Ser Glu Ala Lys Cys 340 345 350Phe Thr Lys Ser Leu Glu Ile Ile Ser Lys Lys Ser Thr Thr Val Gly 355 360 365Cys Lys Lys Glu Asp Pro Cys Val Glu Arg Ser Ser Ser Asn Ala Lys 370 375 380Glu Gln His Asn Ser Asn Leu Ala Ala Ile Ser Ser Gly Phe Lys Val385 390 395 400Arg Asp Leu Gly Lys Ile Leu His Leu Ala Trp Ala Gln Glu Gln Gly 405 410 415Cys Leu Glu Gly Phe Cys Ser Ala Asp Val Glu Gln Cys Cys Gly Val 420 425 430Thr Lys Cys Glu Ser Pro Lys Asp His Gln Ser Pro Pro Pro Val Ser 435 440 445Arg Glu Leu Asp Leu Asn Val Val Ser Val Pro Asp Leu Asn Glu Asp 450 455 460Leu Thr Pro Pro Leu Glu Ser Ser Arg Asp Glu Asp Asn Gly Cys Thr465 470 475 480Leu Glu Arg Ala Thr Gly Pro Asp Asp Asp Ala Ala Ser His Gly Val 485 490 495Glu Lys Asn Gly Leu Gly Leu Ala Arg Ser Asn Gly Ser Gly Pro Ser 500 505 510Asp Glu Ser Gln Ala Trp Ala Leu Ile Arg Asn Gly Asp Val Pro Ala 515 520 525Val Asp Ser Leu Ala Glu Thr Arg Arg Lys Arg Ser Ser Ser Ala Asn 530 535 540Glu Glu Thr His Asp Cys Asp Ser Thr Leu Ile Asn Gly Ser Pro Phe545 550 555 560Arg Ile Ser Gly Gly Pro Gly Ser Leu Asp Gly Asn Phe Glu Tyr Cys 565 570 575Val Lys Val Ile Arg Trp Leu Glu Cys Glu Gly Tyr Leu Lys Gln Glu 580 585 590Phe Arg Leu Lys Leu Leu Thr Trp Phe Ser Leu Arg Ser Thr Glu Gln 595 600 605Glu Arg Arg Val Val Ser Thr Phe Ile Gln Thr Leu Met Asp Asp Pro 610 615 620Lys Ser Leu Ala Gly Gln Leu Val Asp Ser Phe Gly Asp Leu Ile Ser625 630 635 640Ser Lys Arg Pro Arg Thr Ser Phe Thr Ser Ile Pro Ser 645 65010184DNAArtificial SequenceBvAP1 cDNA 10ctagagcaag tgcaacagat gcagtggcag aaccagcacc agcaccagca ccagcagcag 60ccgccaccgc cgccacaaaa tcatcaagtt cctcctgacg catcaaattt catgctccca 120cctccaattc cttctttgaa cacgggtggg taccaaggac aatttggtgg agaagtaagg 180agga 18411150DNAArtificial SequenceBvAP1 fragment 11ctagagcaag tgcaacagat gcagtggcag aaccagcacc agcaccagca ccagcagcag 60ccgccaccgc cgccacaaaa tcatcaagtt cctcctgacg catcaaattt catgctccca 120cctccaattc cttctttgaa cacgggtggg 15012100DNAArtificial SequenceBvAP1 fragment 12ctagagcaag tgcaacagat gcagtggcag aaccagcacc agcaccagca ccagcagcag 60ccgccaccgc cgccacaaaa tcatcaagtt cctcctgacg 1001350DNAArtificial SequenceBvAP1 fragment 13ctagagcaag tgcaacagat gcagtggcag aaccagcacc agcaccagca 5014212DNAArtificial SequenceBvFUL fragment 14agagggagaa ggtgctggct cagcaggcag aattggatca gcaaaatcat gacaataact 60catctggctt tgtgatgtct caagctttgc cctcactgaa tacaggagga acaagcagca 120gtgcagtgga agatgaagca acacaaccac caaatctaaa cagcaactct gcacaaatac 180cgtcctggat gcttcaacac atccaagagc ag 21215150DNAArtificial SequenceBvFUL fragment 15tctggctttg tgatgtctca agctttgccc tcactgaata caggaggaac aagcagcagt 60gcagtggaag atgaagcaac acaaccacca aatctaaaca gcaactctgc acaaataccg 120tcctggatgc ttcaacacat ccaagagcag 15016100DNAArtificial SequenceBvFUL fragment 16aagcagcagt gcagtggaag atgaagcaac acaaccacca aatctaaaca gcaactctgc 60acaaataccg tcctggatgc ttcaacacat ccaagagcag 1001750DNAArtificial SequenceBvFUL fragment 17gcaactctgc acaaataccg tcctggatgc ttcaacacat ccaagagcag 5018399DNAArtificial SequenceBvVIL1 fragment 18cacagctaag tttaaggacc tccacgagat tgttgcagaa gctaaaacaa agctggaggc 60agaggtgggt cctatgaacg gagactctgt caagatggcc agaggtattg ttagcaggct 120tgctattgct gcagatgtgc aaaagctctg ttcgcacgcg attgataaag ctaatgaatg 180gctcgccaat gtttctagca ttagttcaaa ttgcaaagtg gatgcacttc ctgctgcatg 240caggtttcta tttgaagaag ttacttcttg ttcacttgct atagttttga tagatatccc 300cacaccaatg actgattccg tcaaaggcta caagctatgg tactgcaaaa gtagacatga 360gacttttgca agggagccta catccgtctt tccaaggga 39919396DNAArtificial Sequenceconstruct comprising BvFUL fragment and BvAP1 fragment 19agagggagaa ggtgctggct cagcaggcag aattggatca gcaaaatcat gacaataact 60catctggctt tgtgatgtct caagctttgc cctcactgaa tacaggagga acaagcagca 120gtgcagtgga agatgaagca acacaaccac caaatctaaa cagcaactct gcacaaatac 180cgtcctggat gcttcaacac atccaagagc agctagagca agtgcaacag atgcagtggc 240agaaccagca ccagcaccag caccagcagc agccgccacc gccgccacaa aatcatcaag 300ttcctcctga cgcatcaaat ttcatgctcc cacctccaat tccttctttg aacacgggtg 360ggtaccaagg acaatttggt ggagaagtaa ggagga 39620300DNAArtificial Sequenceconstruct comprising BvFUL fragment and BvAP1 fragment 20tctggctttg tgatgtctca agctttgccc tcactgaata caggaggaac aagcagcagt 60gcagtggaag atgaagcaac acaaccacca aatctaaaca gcaactctgc acaaataccg 120tcctggatgc ttcaacacat ccaagagcag ctagagcaag tgcaacagat gcagtggcag 180aaccagcacc agcaccagca ccagcagcag ccgccaccgc cgccacaaaa tcatcaagtt 240cctcctgacg catcaaattt catgctccca cctccaattc cttctttgaa cacgggtggg 30021200DNAArtificial Sequenceconstruct comprising BvFUL fragment and BvAP1 fragment 21aagcagcagt gcagtggaag atgaagcaac acaaccacca aatctaaaca gcaactctgc 60acaaataccg tcctggatgc ttcaacacat ccaagagcag ctagagcaag tgcaacagat 120gcagtggcag aaccagcacc agcaccagca ccagcagcag ccgccaccgc cgccacaaaa 180tcatcaagtt cctcctgacg 20022100DNAArtificial Sequenceconstruct comprising BvFUL fragment and BvAP1 fragment 22gcaactctgc acaaataccg tcctggatgc ttcaacacat ccaagagcag ctagagcaag 60tgcaacagat gcagtggcag aaccagcacc agcaccagca 10023795DNAArtificial Sequenceconstruct comprising BvFUL fragment, BvAP1 fragment and BvVIL1 fragment 23agagggagaa ggtgctggct cagcaggcag aattggatca gcaaaatcat gacaataact 60catctggctt tgtgatgtct caagctttgc cctcactgaa tacaggagga acaagcagca 120gtgcagtgga agatgaagca acacaaccac caaatctaaa cagcaactct gcacaaatac 180cgtcctggat gcttcaacac atccaagagc agctagagca agtgcaacag atgcagtggc 240agaaccagca ccagcaccag caccagcagc agccgccacc gccgccacaa aatcatcaag 300ttcctcctga cgcatcaaat ttcatgctcc cacctccaat tccttctttg aacacgggtg 360ggtaccaagg acaatttggt ggagaagtaa ggaggacaca gctaagttta aggacctcca 420cgagattgtt gcagaagcta aaacaaagct ggaggcagag gtgggtccta tgaacggaga 480ctctgtcaag atggccagag gtattgttag caggcttgct attgctgcag atgtgcaaaa 540gctctgttcg cacgcgattg ataaagctaa tgaatggctc gccaatgttt ctagcattag 600ttcaaattgc aaagtggatg cacttcctgc tgcatgcagg tttctatttg aagaagttac 660ttcttgttca cttgctatag ttttgataga tatccccaca ccaatgactg attccgtcaa 720aggctacaag ctatggtact gcaaaagtag acatgagact tttgcaaggg agcctacatc 780cgtctttcca aggga 79524845DNABeta vulgaris 24ctaggtcaga ttcgctatct atcttcttct tcttttttgt tggtcaatca ctctgaagaa 60cctttatgac aagtagtatt aagtttcatg aacttctagt ttaaactcgc tctaaaaata 120ctaaaagatt acatttcaat atatattcct gtgttatata tacacatgct gttttgtagt 180aaatattatg tgaagttggt cttaaaattg acatgatgta aatatcacgt cagtttttat 240aaccgattca aattaaacat cagttataac aagcgatgca aatattatgt gaagttgcat 300cagttgttaa taaatcgatg taaattattt gcatcaacaa gatacgaatc gtttatttaa 360gtgatgtaaa atttctttac atcaattata agtaatacaa atattcaata taagtgattt 420aaaatgatat tttttttgta gtgatcccat gtgaagttat aattcattct ttcagcatca 480tagtctcttg gagttttctc tttgtcctca ccacacttat tattctcttc ccttttaact 540atcaaaagat accctcccct taactttaag attttaaatt aagaaatcgt agacacgaaa 600aatctaagaa ctaacaaata ttttgataaa gacagcttct aaaattcaat atctgaatag 660tatctcttgg aaaatgtcgt gttgtggtcg gtcacatttc aacactcttg tacaaaagcg 720tcaacttgac ttcatgtgac agtttttgtt tattagagat gtttagttgt agaattgatg 780atttatgtat ataacaacga tattctatag atatatattg atgttaaaag ataaacagga 840ggaac 8452529DNAArtificial Sequenceforward primer WAG-AP1FUL 25ctcgagagag ggagaaggtg ctggctcag 292631DNAArtificial Sequencereverse primer WAG-AP1FUL 26cccgggtcct ccttacttct ccaccaaatt g 312731DNAArtificial Sequenceforward primer vil1RNAi-fwd 27gtcgaccaca gctaagttta aggacctcca c 312829DNAArtificial Sequencereverse primer vil1RNAi-rev 28ctcgagtccc ttggaaagac ggatgtagg 2929411DNAArtificial SequenceVIL1 cDNA fragment for cloning of VIL1-AP1-FUL RNAi construct 29gtcgaccaca gctaagttta aggacctcca cgagattgtt gcagaagcta aaacaaagct 60ggaggcagag gtgggtccta tgaacggaga ctctgtcaag atggccagag gtattgttag 120caggcttgct attgctgcag atgtgcaaaa gctctgttcg cacgcgattg ataaagctaa 180tgaatggctc gccaatgttt ctagcattag ttcaaattgc aaagtggatg cacttcctgc 240tgcatgcagg tttctatttg aagaagttac ttcttgttca cttgctatag ttttgataga 300tatccccaca ccaatgactg attccgtcaa aggctacaag ctatggtact gcaaaagtag 360acatgagact tttgcaaggg agcctacatc cgtctttcca agggactcga g 41130408DNAArtificial Sequenceconstruct comprising BvFUL fragment and BvAP1 fragment for cloning of VIL1-AP1-FUL RNAi construct 30ctcgagagag ggagaaggtg ctggctcagc aggcagaatt ggatcagcaa aatcatgaca 60ataactcatc tggctttgtg atgtctcaag ctttgccctc actgaataca ggaggaacaa 120gcagcagtgc agtggaagat gaagcaacac aaccaccaaa tctaaacagc aactctgcac 180aaataccgtc ctggatgctt caacacatcc aagagcagct agagcaagtg caacagatgc 240agtggcagaa ccagcaccag caccagcacc agcagcagcc gccaccgccg ccacaaaatc 300atcaagttcc tcctgacgca tcaaatttca tgctcccacc tccaattcct tctttgaaca 360cgggtgggta ccaaggacaa tttggtggag aagtaaggag gacccggg 4083125DNAArtificial Sequenceforward primer for VIL1-AP1-Ful 31gtcgaccaca gctaagttta aggac 253225DNAArtificial Sequencereverse primer for VIL1-AP1-Ful 32cccgggtcct ccttacttct ccacc 2533780DNAArtificial SequenceBvAP1 cDNA showing sites for mutagenesismutation(1)..(1)any nucleotide except amutation(2)..(2)any nucleotide except tmutation(3)..(3)any nucleotide except gmutation(52)..(52)c to tmutation(151)..(151)c to tmutation(262)..(262)c to tmutation(316)..(316)c to tmutation(343)..(343)c to tmutation(376)..(376)c to tmutation(388)..(388)c to tmutation(391)..(391)c to tmutation(418)..(418)c to tmutation(433)..(433)c to tmutation(463)..(463)c to tmutation(484)..(484)c to tmutation(541)..(541)c to tmutation(547)..(547)c to tmutation(550)..(550)c to tmutation(556)..(556)c to tmutation(562)..(562)c to tmutation(568)..(568)c to tmutation(574)..(574)c to tmutation(580)..(580)c to tmutation(586)..(586)c to tmutation(589)..(589)c to tmutation(592)..(592)c to tmutation(610)..(610)c to tmutation(619)..(619)c to tmutation(688)..(688)c to tmutation(694)..(694)c to t 33bvhggaaggg gtagggttga gctgaagagg atagagaata agatcaatag acaagtaact 60ttttcaaaga gaagaagtgg acttgtgaag aaagctcatg aaatttctgt tctttgtgat 120gctgaggttg ctctgatcat tttttctcac tgaggaaaac tctttgagta ttcttctgat 180tcttctatgg agaagatcct agaaaggtat gagaggtatt cttacgcaga aagacggcta 240gcttcaaatg atccagactc ataggtaaac tggacctttg acttcgcaaa actgaaggcg 300aagcttgaac ttctataaag gaatcatagg cactacttag gataagagct tgactcgctt 360aacatgaagg aactttagag tttagagtaa taacttgata ctgctctaaa aaatgtttga 420tctaggaaga actaactgat gcacgagtcc atttctgaac tctagaagaa ggagagggca 480atgtaggagc acaataacat cctgtctaag aagatcaagg agagaggaaa aaatctagag 540taagtgtaat agatgtagtg gtagaactag cactagcact agcactagta gtagccgcca 600ccgccgccat aaaatcatta agttcctcct gacgcatcaa atttcatgct cccacctcca 660attccttctt tgaacacggg tgggtactaa ggataatttg gtggagaagt aaggaggaat 720gatcttgacc tgacgctaga accgatatac tcatgtcaca tgggatgctt tacaacatga 78034735DNAArtificial SequenceBvAP1 cDNA showing sites for mutagenesismutation(1)..(1)any nucleotide except amutation(2)..(2)any nucleotide except tmutation(3)..(3)any nucleotide except gmutation(19)..(19)c to tmutation(52)..(52)c to tmutation(235)..(235)c to tmutation(316)..(316)c to tmutation(325)..(325)c to tmutation(376)..(376)c to tmutation(391)..(391)c to tmutation(433)..(433)c to tmutation(457)..(457)c to tmutation(463)..(463)c to tmutation(466)..(466)c to tmutation(541)..(541)c to tmutation(544)..(544)c to tmutation(559)..(559)c to tmutation(562)..(562)c to tmutation(601)..(601)c to tmutation(664)..(664)c to tmutation(694)..(694)c to tmutation(715)..(715)c to tmutation(724)..(724)c to t 34bvhgggagag gtagggttta gctcaaaaga attgaaaaca agatcaaccg ttaagtgacc 60ttctccaaac gtcggattgg attgttgaag aaagcgcacg agatctccat tctctgcgat 120gccgatgtag ctctcatcat cttctccact aaaggcaagc tcttcgagta tgcttctgat 180acctgcatgg aaaggatact cgagcgctat gaaagacact catatgcaga gagataactg 240actgctccag atcctggatc ccatgtaagt ttgactctgg aacacgcaaa acttaaggct 300aggctggaca ttctttagaa aaattaaagg cattacatgg gagaagaact tgataccttg 360agtctcaagg agctttagaa tttagagcat taaattgaca gtgctcttaa acacatcagg 420tcaaagaaga actaactcat gcatgaatcg atttcttagc tttagtgaaa ggacaaagcg 480ttaaaggagc acaacaactt gctatccaag aaggtgaagg agagggagaa ggtgctggct 540tagtaggcag aattggatta gtaaaatcat gacaataact catctggctt tgtgatgtct 600taagctttgc cctcactgaa tacaggagga acaagcagca gtgcagtgga agatgaagca 660acataaccac caaatctaaa cagcaactct gcataaatac cgtcctggat gctttaacac 720atctaagagc agtaa 73535780DNAArtificial SequenceBvAP1 cDNA mutation position 262mutation(262)..(262)c to t 35atgggaaggg gtagggttga gctgaagagg atagagaata agatcaacag acaagtaact 60ttttcaaaga gaagaagtgg acttgtgaag aaagctcatg aaatttctgt tctttgtgat 120gctgaggttg ctctgatcat tttttctcac

cgaggaaaac tctttgagta ttcttctgat 180tcttctatgg agaagatcct agaaaggtat gagaggtatt cttacgcaga aagacggcta 240gcttcaaatg atccagactc ataggtaaac tggacctttg acttcgcaaa actgaaggcg 300aagcttgaac ttctacaaag gaatcatagg cactacttag gacaagagct tgactcgctt 360aacatgaagg aacttcagag tttagagcaa caacttgata ctgctctaaa aaatgttcga 420tctaggaaga accaactgat gcacgagtcc atttctgaac tccagaagaa ggagagggca 480atgcaggagc acaataacat cctgtctaag aagatcaagg agagaggaaa aaatctagag 540caagtgcaac agatgcagtg gcagaaccag caccagcacc agcaccagca gcagccgcca 600ccgccgccac aaaatcatca agttcctcct gacgcatcaa atttcatgct cccacctcca 660attccttctt tgaacacggg tgggtaccaa ggacaatttg gtggagaagt aaggaggaat 720gatcttgacc tgacgctaga accgatatac tcatgtcaca tgggatgctt tacaacatga 78036735DNAArtificial SequenceBvFUL cDNA mutation position 316mutation(316)..(316)c to t 36atggggagag gtagggttca gctcaaaaga attgaaaaca agatcaaccg tcaagtgacc 60ttctccaaac gtcggattgg attgttgaag aaagcgcacg agatctccat tctctgcgat 120gccgatgtag ctctcatcat cttctccact aaaggcaagc tcttcgagta tgcttctgat 180acctgcatgg aaaggatact cgagcgctat gaaagacact catatgcaga gagacaactg 240actgctccag atcctggatc ccatgtaagt ttgactctgg aacacgcaaa acttaaggct 300aggctggaca ttctttagaa aaatcaaagg cattacatgg gagaagaact tgataccttg 360agtctcaagg agcttcagaa tttagagcat caaattgaca gtgctcttaa acacatcagg 420tcaaagaaga accaactcat gcatgaatcg atttctcagc ttcagcgaaa ggacaaagcg 480ttaaaggagc acaacaactt gctatccaag aaggtgaagg agagggagaa ggtgctggct 540cagcaggcag aattggatca gcaaaatcat gacaataact catctggctt tgtgatgtct 600caagctttgc cctcactgaa tacaggagga acaagcagca gtgcagtgga agatgaagca 660acacaaccac caaatctaaa cagcaactct gcacaaatac cgtcctggat gcttcaacac 720atccaagagc agtaa 7353721668DNAArtificial SequenceBvAP1 genomic DNA showing sites for mutagenesismutation(1)..(1)any nucleotide exept amutation(2)..(2)any nucleotide exept tmutation(3)..(3)any nucleotide exept gmutation(52)..(52)c to tmutation(151)..(151)c to tmutation(6999)..(6999)c to tmutation(7640)..(7640)c to tmutation(8573)..(8573)c to tmutation(8606)..(8606)c to tmutation(8618)..(8618)c to tmutation(8621)..(8621)c to tmutation(8648)..(8648)c to tmisc_feature(9833)..(11385)n is a, c, g, or tmutation(11796)..(11796)c to tmutation(11826)..(11826)c to tmisc_feature(12467)..(12605)n is a, c, g, or tmisc_feature(13741)..(14088)n is a, c, g, or tmisc_feature(16396)..(17194)n is a, c, g, or tmutation(21152)..(21152)c to tmutation(21313)..(21313)c to tmutation(21319)..(21319)c to tmutation(21322)..(21322)c to tmutation(21328)..(21328)c to tmutation(21334)..(21334)c to tmutation(21340)..(21340)c to tmutation(21346)..(21346)c to tmutation(21352)..(21352)c to tmutation(21358)..(21358)c to tmutation(21361)..(21361)c to tmutation(21364)..(21364)c to tmutation(21382)..(21382)c to tmutation(21391)..(21391)c to tmutation(21576)..(21576)c to tmutation(21582)..(21582)c to t 37bvhgggagag gaagagtgca gctgaagagg atagagaata agatcaacag ataagtaact 60ttttcaaaga gaagaagtgg acttgtgaag aaagctcatg aaatttctgt tctttgtgat 120gctgaggttg ctctgatcat tttttctcac tgaggaaaac tctttgagta ttcttctgat 180tcttcgtaag tatatatata tatatattaa tagtaactac ttgttttctg ctttctattt 240ttaggtctga tgcatattta atttaggtaa tattaattcc ttatatctga tccttaattt 300ttttttcttt taccatttca tttttgtttg ttttgaataa aagaaaattt ccccttcacg 360tgtgtcgaat aggtcaaaat ttttacttga aggatgttct ctttgattac taaaatagga 420tccaacaatc acctgaaata aaggaagaag atggtgcaaa gtttttactg tcatacttag 480tatttgataa atattctatg atgaacttgt ataaattagg aaatagacct aactttcatg 540cacgaaaaca ttattccttc attcaatttt tttattactt aaggatttac ttttttattg 600atcatatgaa gtagtagtac ttgtaatcat tcaatttttt tgttggttaa ataggactac 660attttaaaac aacccaattt taaaattttt tgtgtgaatt tcttcccttt ttaaaaataa 720agtctattat catagcttag agtagctgtg gcaaagctag acgaaataat acagaaatct 780ggaaaggaaa ttgtactact tacatgaaca cacttattta ttacttgcat gatatctgcg 840aaaaagttta tagcaaattt ggttaatata tagcgtagta ctttggatat taatattact 900agtgtacaaa tacttgatcc aatgggtaat gaaacttatg gaagatttga ccatacatga 960tgatgctaaa tattaattgt tattgtccag ctttgttttc cctccatcca ttggcatctt 1020catctttaca ttgctactcc actcacttgt caattgtttc gtcctttatg ttctttattc 1080acatgtgcac catacttcaa tactttcccc ttctttatcc tcagtttttt tttcttgtca 1140ttttagggtt aatatccaat gaaatctagt ttgctcgttt tagatctaat tttaattcga 1200tcacaaccat ccatattttt gtttcttagc ttgacatcta ttctatggat ctgggatctt 1260cggtgtatag atgttctcgg ttttcagatc aagatcctat tcatagaccc atttattgta 1320aacacttaaa tgtgttctta aaaagttagt ggctcgccaa gtcaactcaa taacataacc 1380cccacgactt cattacatta cacaatgaaa gattagatgt atgagtttgt gaagcttata 1440attctatttc aagtaggact aggatgtttt gtgcaatcag cagctagtag tctttttaat 1500ttaagtcagt cttcattgtg catcatatat ttttagaaat atatgcaagt ttgaaaccat 1560ttagaacctc atgacccgcc tgactcacta taaaccggca agagcttaat ttttcacagc 1620tttgtatctt tatgagtagc gctagctagg ggtatgggca tagaaaaaaa gggtttgggt 1680tagggtctta caagatctta tccgctattt ttatttcata atctttcaaa atacatgttt 1740aataattcaa aatacatgtt taatactatc tccatttcac aacatatgca ccaattgcct 1800agctatggtc caacctagtt ggtttgtagc ttgcattgga tggttaggat gtattggagt 1860tgtttatgtg caatcaaatt ttaattacgt atcaaaaaaa aaaaaaaaaa aacatatgca 1920ccaatttcca tttggacaca cttattgacc aatttttgac aatatttttc tcaccatttt 1980gtaagaaaaa tcaaaatcaa gtggaatttt gttaagttta tctcagtcaa aagattccat 2040acatcgacat tttataattt ttaatcatac gcaattagaa atatcaatgt ctaaagaagc 2100gtgttggaat acgtgaaaaa gcaaatgata catgaaacag atgtagtata tagaaaactt 2160aattttgtgt cactcggatg tatgtgggcg gagccttcct agaaggcgta cccaccttag 2220tggctctgaa tctttgacga cccgttcggt tggtggtgat aatagatggt aatagtaatg 2280taatttagtc taaatttata aataaatatt aatatcatta cccatggtaa tacaagttct 2340tcacaaaaca tgtttcattt aaaaattatc attactacct tttcaagtgg tattggatga 2400taataaaatt ttaggcaggg aaatgggtat tgggatgaac attaccatgg gtaatgacat 2460gcaatttttg ttacaagaat acagtataat acattactat tgccaccatg tataaccatt 2520aatcaaatgg accgtgagga tatgatgttg aagaagaagt cttaacctct acgctattat 2580ttactagggt ctgtaaattt tcctttttta attataattc ttgtgaaatc ttcttcactg 2640atggtactag cttattagga tgggtttctt tagtatattg aaggctcttg ttgacagagt 2700ataaaaatat ttttggggtc gcaaccatca atttaaactt ttgtttgatt ataaaattat 2760tttttgaaca tcaacaatct acttaaattt ttggttgagt tagttctttg acatggtatc 2820acaaccatca tgacataaag gtctcatatt caaatctcat tcacctctca tttccaagta 2880gaatatttac ctcaggtatg ggtatgaggg aggcttgtgt tgcatgagtc aataacggat 2940cttgaccaat aatttaacag gggcgagttg ataaattaag ttttaatgta aaattttaaa 3000tgatggataa aaacactaat acacaccaaa atataaatat acttttatta atggttacaa 3060agagcttgta gctaatgtaa taaatcaaaa tcccaaaggt gcaattttta agaaattatt 3120tccatttatt tatttgacca ttatgaaatc ttcaagaaat tgagtaagtt tttaagaaat 3180ttaaggtata gttcattaac taaataaact actccagtaa aaaaaaatta ccaaactgct 3240ttcttaagta aaaaaataaa taaatttata ttttatgatt gttaggaatg agtgtgagga 3300aataaaaagt actattatag ttaaataaaa atgaaagttc ttcagagaag aagaatagaa 3360gatagtacaa tcaatgttaa atatttttct aaattagaca aattgatata aaccaaaaat 3420aaaggggaag aagaaagaaa taagtaaaaa aagaaagaag gaaaagaaaa aaagaaaaaa 3480gagaagcaag tgaagaaaaa caaagaagtc caaatgtgtg ttgatgcaag gttcgagctt 3540gcaacattaa gggctcaaac tttcttttac actttggttc actgccaccg tgcccacagc 3600ttgttatgtg acatgaagtg tagtttgctt aatttatctt atacagttat gggggaccaa 3660gcctccaccc gccccttcta taatctgtca gtggttgcat ccacacttta agtccaatag 3720actcttgtct gagaggaggt gatagagtat ataaatattt ttggggcctc aaccattagc 3780tcaatctttt gattaagttg gttctgtgac acttgtacta tatactagtt atatatatac 3840tgtaaaacta gtaccacgag aacagtcctt aatacaaaca acatgccctt aatagaattt 3900tcttagtata cacttaatat aggttgacta gctttttgcc cttcagtatg cacacacctt 3960ttataatctg tatcgttgtc tggtagatga taataaacct cagtattggc aatatatgaa 4020atgacataat ggccatgttt ggtgattaga gtttagagtt tagaggttac agttcagagt 4080ttgtggttag atgattactt ttttgttcag aggatttgac tgctgattta aataattgtt 4140gtgtaaaggt gtttggtaac acttagctta ttgtttagag ttttgtactt tttagagcat 4200gtaaaatgac atttatggac atatgtattt ttttaaaaca aattttagta gtaattatat 4260ggacaaaata gtcatttgtt ttttctctct ccaaaactct catgaaaaag ctcctctacc 4320cagctttttc aaaagagagt tttgatcaga gttttcggta caaaactctc tttagtcctc 4380tctctcacca aacacccaaa ttagagtttt tattggtcaa aactctaaac tctctccaaa 4440cctctaaact ctctctaaaa ctctctcccc caaacacccc caatttctta gaaaaatttg 4500ttgctccttt ttattgcact atatttctat ctccaaacat aaagtttctt ttacaaattt 4560tcatttctac tccataccac ctttatatgg caatataatt tctatgaatt aaaatgttca 4620caagttttga ggtggatttc aagagcatgg acaatatgat catgagactc tccatacaaa 4680aattaccctt aaattttata atcatacacc aagcggtcgt taaagtattg gaagtgcttg 4740agtagtttgt gaaaattaac atataataaa gtgcagatct cccctctagt aagtagtaag 4800aagtagtaag acgatgtccc tcatttgaga aagagaaaaa cccttatcag tttctcttgt 4860ttctttgact gaacgcaagt caaatagaag tatgtaacta ggaaatcctt ggagaaatag 4920attttcttta aaactataaa agtataccta tatatatggt aacccacaaa aatgtatata 4980atctgatcaa tatctaaaca aagtattctt atgttttctt tcatcttgct tatttcctcc 5040ctttcctttt cttactttaa tttgtttact ctctttaact tatttctttg cgtatctcat 5100ttcactttac aaggatatat agttgattat gacagcttaa taaatatatt ttggaactag 5160gatttattgg ttgtcgttgt tattttaatt tctacactga tcggctagag tttctagaac 5220atagggcttt attgaaacca ttagttaaca aaattgaatg acaatgattc aatatgatag 5280aatatgtatg tattagttaa tgtttgatta ttgtttgtat gtatataatc aaagattatt 5340tagtaatact tctatataca tattctatta gaatcactta gaaagaccca ttgaacaata 5400ataaggatag gcagacaagc aaacaaaaga aaaataaacc tgttactcct tccatttctt 5460aatgttctac tcggaattat agatacacac tttgacacaa attagaaaga gagtgtaaaa 5520agtggatcca tattaatatt tttatttttt taaatgagga gagaagtgtg ggtttattat 5580gtttcaaggg agatagagag cattgaatag tgagagaata tgtgccaaag ataattaaat 5640cattgtaaat cttttgccaa ataaagaata aagcatgtga gtaaaacttt aaaaaatggg 5700cgaaaaagga aagttgagta gaactttaag agacggaagg aatatagaag aggacgtgac 5760agatgggagg aagatcagac atcttagaag gggaatagtt aaatttgaga tagtctttta 5820attaaggttc tcactaaaga agatataaca gtaggggaaa gctaaaggtt attcaaaact 5880ttccttccca tcttcatcac ttcatgtctt tactttagag ctcttaacac ttagcctatg 5940aaattctgaa ctctttgtaa gattagtgat agataaaaga atcttatcaa tttaatttat 6000aaatacaaca ggattcaata aaaagatata gagatctata aataaagagc catactgttg 6060tgaactttta tatctatcaa aacctttgca cattagacgt ggtataacta aatcaggctt 6120atcgaaattt tttaaaattg ttttcattat agccccttta tatttagaag ttctaagatg 6180attgcataga tagttgatgc accgttctgg tcgacttttt taaacacttc tttttgataa 6240attttttttt ttgtattcga atcattattt taggtgtata aagagctgca aatgatctag 6300atgagattga tctcggtttc atttatatgc taatagtgtg ttagatacac actattaaaa 6360aagtcatatg acttagagat tattatggaa aagggatagt gcaccgatat taatataatg 6420gaaaatgaca cacgagttgt ccataataac atgtgaaaag tgaactattt aaaaggtttt 6480tctgacctag tacatacaag gtgcgtaggt ttagctattt tagtttttta gttttatttt 6540ttaaagtgaa gttagttatt gatctgaaat catataacat gtacgtaccg tagatataaa 6600aaactaccaa gtatatatca atttgaaata aacattattt taatatggca aaatcacaat 6660tgttgactag acctaacact gaagaaaact atgtcatgtt tatcaattat gttgcataca 6720gttaaaaaca aatatgttag agaaatcgtt atttgaaata gaaaagttgc gcaaaatagt 6780gattaacatc aaaatatgtt cagaaagttt ttataaatat gtgatcttgc attgtctgtt 6840gactgtcgag gtttatgata atttcccctt tttccaatgc aaaacttgtt gtgctatttt 6900ctaatgatat attttttcaa agtatggaga agatcctaga aaggtatgag aggtattctt 6960acgcagaaag acggctagct tcaaatgatc cagactcata ggtagtgcat ttatgtaaat 7020atagatatac tcttcatgcc caagaagcct gaatttttta tcccactacg tactgcaaag 7080ccaagtttaa ttgaataatt gtcctgttta aattatttag ttttcagtac aataatgtaa 7140tcattagttt gcatgtttaa aaaagaaaag cacaagttct gatcaagtga aatataaatt 7200gtaacgaaag agccaagcta gacaattacc tagctaggag ttatttgtta tcgtttttgt 7260ttttaatttc tagttttttt ttttaaacta gaaaatatag tttcaatctt ttgttatcag 7320ttttcaaaat gacatattta acataaatat gattgatttt aaattcattt attatatcat 7380atttcatttc aaaataagtg aaacacttgt ctcaaaaagc tcactctcac ataaatgata 7440aaagtgtttc acttatttta aaacggaaga attatgactt ttacttttca taaaacgaaa 7500aactgaaata tgacaataat ctcaaatagc ttggagaaac cagatttcta tatatttccg 7560tgatgaaatc acttttcatt atacgtaggt aaactggacc tttgacttcg caaaactgaa 7620ggcgaagctt gaacttctat aaaggaatca taggtatgat ggcaatatgt cataattttt 7680ctattattat ttttgcttcc aaaaccagac catatgtttg tatatttata tagtgatata 7740ctccatccgt ttcattttaa tctatacatt tacacttatc aggtatgtca atgcaaaatt 7800ttgaggatat atatctttag ttttgtattt ataaaaatta taaaaagtac atattaataa 7860aatacatatt atgatgaatc taacaagatc ccacatgacg atatttccgt ccgcgtatga 7920ataacaaata atggccaaag tgaaatttgt gaatagtgta aaatatcaaa gtgtaacaat 7980taaaataaaa cggagggagt agtacttgtt tgtcacatac ttacttattt ttgttctctc 8040cacaatgaaa ctgttctttc taataattaa aaaaagtgca tatgttgatg atttctctgt 8100cactttaagt ggatattgaa tagtgataat ggattacttt gtgtataatt gcatttcaca 8160tttgggtcta attttatacc cttttcgcat atcatgcttt gtgaatagta catatgatgt 8220tcaagaatgt gagaagacat atcatacttt tgatatacct caaacatggg tgtatactgt 8280atagtgaacg aaagtgttag tgtaatttta tttaggaggt ttagtggttt gtcctatata 8340taatgctagt agttatacac catagttgtt gatgagcatc aactggcttt cctaacattt 8400ttttctccat aactttaccc ttaccttaca ttagattact ctaggattac attctaccta 8460aaatattatt actcccatca ttttaggtaa atatttttac tttgattttt cgattatttt 8520caagagttta aaataatgat taaaatttac catgatcagg cactacttag gataagagct 8580tgactcactt aacatgaagg aactttagag tttagagtaa taacttgata ctgctctcaa 8640aaatgtttga tctaggaagg taagaaattt tacttgtcta ccgtagtttc ataataaatt 8700agtatttggg ctcgggcttt gccccagatt ggtattgtct tttcaaattt gatatgcatt 8760tttttccatt tccactaaaa tatattaaga aaattcaaca tttaaaggat acaaatataa 8820taatgtggat acttaaagta tgattaaaat ttggttgaga tggtaattgt gtcatgtata 8880atagcaagaa gtcacaagtt caaagctcgt tgcaagctaa atttattttt gttgattgac 8940atgacttatc aacacactgg acaattctaa tcatctagtg gagtagcata tactagcaat 9000ttatgcacgt gatgtgtgcc ttactttttt agaatataat ttataacttt tttgagcata 9060aacaaaggta aaatttgaac attagacata tttttttggg ctagctaaat ttgttgttta 9120aacctatatc acttaaccaa actcctcttt tattatttat tgatttatat tttatttaaa 9180atttttaaaa ttaaaatgat gagcaataaa agaatgttaa gtagatttat taagtatttc 9240ttatattttt atcaacaaag tattttgtgt taattaaatt atttcacttt gttaattgat 9300tgtattttcc tttttaattt attacttgat tgtgtattga ttgatcaaac ataatttttt 9360tgttaatttt tttatgctat atttgaattt atttttcttt catctgtttt tggtagagta 9420gttgatttac taaagggtaa ttaaataaat ttattggggg acaccatagc tcccccctcc 9480cttatataat agagatttgt atagatttat tgtcttcctc aattattgat taactagtct 9540tctatgcacg cgatgcgtgt gttgattgtt tgggtctatt cttaatataa atttcatcaa 9600aatataatta tagtagtgtg atttacaatt attgctatac aaactactgt aatttataaa 9660gttgttagaa attgagataa aaatttagat gtgaaatttt gtggtcaaat tatatttgta 9720attttttaaa ctgagtaacc gtttttctca tcatgtcaag ttactttgtt aatgcttatt 9780taatttatta ttggaatttt tgacccatct ttaaattaga aaaggatata atnnnnnnnn 9840nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 9900nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 9960nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 10020nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 10080nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 10140nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 10200nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 10260nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 10320nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 10380nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 10440nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 10500nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 10560nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 10620nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 10680nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 10740nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 10800nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 10860nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 10920nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 10980nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 11040nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 11100nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 11160nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 11220nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 11280nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 11340nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnntatat atatatttag 11400ctaagaaaaa aaagacattt cattggggga taccataact cccccttagc ttatataata 11460gagatactat acttcttttc tgatagtgtc aaatttaatt ataaatcttt aacattggcc 11520aattaataat tggacaagaa aaaaatgaga caataataaa taaggcgatc ttcacagacg 11580tattaacatg atggtaatta aaaatgttaa tcatagatct ttgtgttatc ttaataatat 11640aaatttacta attagaatgt atcacataaa gtaagtatta atagcagcat aggataattc 11700ttataatgga gattttatat ttttttatat aattatatga tttattgttg aaaatattag 11760ttgattttaa ctggttgttt attcaatgac agaactaact gatgcacgag tccatttctg 11820aactctagaa gaaggtaata actccatttt ttactctcaa aggtttattg tttttaactt 11880atttcttcta accttttata tatgagaagg tattgggtta gacgcgtctg accataatat 11940taggtcggat gactttcagt tggtttcaat tttatttcag ttggtttcaa tttttgtcca 12000gttggtttca atttttgttc agttggtttc aatttttttt agctggtttc aatttttgtt 12060cagttggttt caatattttt tagttgatct tttttatttc agttggatgt cttttaagtt 12120cagttactta tcttattgtt tcatttacgt gttttattgt aactgaaaac aaaacttaag 12180taaatgaaat aaaataagtt ctaaataaaa gcaacttagg gcctgttctc cccagcttat 12240tttcagttca gttcaattca gttcagttca attcaattca tttcagttca gttcagatca 12300gatcagttca gttcagatca gatcagttct tgacaatact tttactctca catatcacta 12360ttcatttcag ttcagttcaa ttcaattcag

ttcagttcaa ttcagtttag ttcagttcag 12420ttcagttcaa ttcagttgtt ttatgccgaa gagaacaggc ccttagnnnn nnnnnnnnnn 12480nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 12540nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 12600nnnnnattca tttcagttca gttcaattca attcagttca gttcaattca gtttagttca 12660gttcagttca gttcaattca gttgttttat gccgaagaga acaggccctt agttttcagt 12720tacttatatt atcgtttcag ttagttttct tattctttca tttaacaact aactaaaata 12780aaaaaaaaaa aactaactga aagcaaaact taattaaatg caaaaaatta agttctaaat 12840gaaaccacat acgatcgaaa tttcaatcat ttcaaacatt atggtgtttt cgattctttc 12900aaagaaggca agctgctccc gctattctac cctctttaga tcacaataaa gctcaggcct 12960cacattcaaa gtttcctcaa agatggacgt tccaagtatc acatagacac atagtcctct 13020tctccaaacg ctctccttcc tatcttgatg tcattagcaa acttcttgat ccagacggcg 13080ccaacaaccg caccatgatc tccctctaaa gtactgacgg cccgtttggt tgttggtcat 13140aaatgatggt aatgggaatg aagttgtgtg taaatttgtg aaaaatatca ttgtccattc 13200ccatggtaat gctaatttat cttaatgtgt ccactttcct tctagaattt tcattctcat 13260ccaataccac cttgtaaggt ggtaatgagt ggtaatgaaa attgcttccc cttggagaca 13320aaaatacaag tttaggagtg agattgattg ctcatggaga aaaaaagtct ccccatggag 13380atattaaggg tgattcccta ataaaattac acttaaaatt tattcccatt accgcaattt 13440attaacatct accaaacggg ccgtgaaagt cttgaaacac atagtcgagt gagtagcttt 13500gaggaaccat ctgtaaaaga acctgaggga gccaatgtgt gcgtaagtac caacggcgtg 13560ttgtcagtgg aaaaggtggt gccgtggtgg cactcagtag tgatggagcc gccgtggtgt 13620ttgagtgttg ccaaatacaa aggcggaatt tcgtaatctc taatttcttc tgtgaaattt 13680ttgggatcag cctgtccgac caaacacatt ggatcaaacg gtctgaccca atagtttcaa 13740nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 13800nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 13860nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 13920nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 13980nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 14040nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnga tataaataga 14100gatggaggac aagggtcttg gttttgtcat gttgtcaaag agttgaacaa tggttttttc 14160gtattgttaa aaaattaaaa aaccagaggc cttcaatctc ttgatagata tagatagaaa 14220aggaggacaa ggccgttctt ggttttgtta tattgtcaaa gagttgaaca atgatttttt 14280cgtgttgtca aaaaattaaa caatgaaaaa agatggcggg tgcttgatct aatagatcgg 14340accatggatt gaaggtcttt taacttattt tatatatata ttgaacttat ctgaagatta 14400tttaactctt tgaaattgta ttaacttccg aactttatga acttttttaa ttcttcaaaa 14460cttatctaca ttttatttga aaaaatattg aagacaaaaa aaccctcagt tggtttaaag 14520ctgcggtaag atagagtgta aatgttattt ttttttatta aatcaagaaa taaaaagaaa 14580tattaaataa aaagaattaa aaatggaaat gatgacagaa acttatggct tggaggagca 14640atacttttaa gatagaccta aaccttaaat aagttaaaat ggaagtaatt tttcagtaga 14700atcttattcc aatctatact ccgtgtttac tccatgtaat gcacatataa taaaaaaatt 14760agaaattaca tagtataagg tttgatcctg tgactgtaag tttatatact aacttcttaa 14820ccactagagc aagtgatatt tagtgttatc attttaaagt ataattttaa caaatgaaat 14880ttttttctta cccggaacat agctcggacc taataactag ttgaacaatt ataatctgta 14940acttaaaatg atcctaatta ctgtactttc attacctata ataatagaat cttactatca 15000ttggttcaga aaaaaaaaat cttattaaat gttaaccatt tatttgtaat tgaaacatac 15060atgcacataa atgtaacttt tagtttatct taacttaaaa actgagaaaa tgttagttgg 15120aaacttttgt atatatgttt ggataaacga cgctcaaaag taggggctaa aattttagta 15180gataatataa gattatactc catctgttct agatagactt ctcattttta attttggcag 15240tattcataaa taaaggaaat ctttcaaaaa aatttccaat atataagaaa aaaaataatc 15300atgtgcggtt ttgtttgatt cgtctcattg tgtacattag gaaaattaaa cttatataat 15360ttttactact atgtaattaa agatattaac gatacaaaat gtgtattgac aaacttatat 15420tggagtaata ggaagtctat taagggaccg aagaaatatt acgtaaataa atctaataca 15480aactaatata aattctactc cagacaataa agattctgtc ttatattgcc aagatatagt 15540agctatttat tttatcttaa caaacataaa tgtttctaat gcttaaacat ggacatgtat 15600tattttgtaa aatattatgt attatccaaa gttacatatt taaaggaagt tctattgctt 15660gctctctttt agcactgccc aaaaaggtta aagtaatttt ttttctctgt ttaaaaaaaa 15720aatgcattat atacagataa tttttgctag tcaataaagc tatccttatg acttatgagt 15780gctacttgac tagggatgtg ttgtactcaa ttggaggtat acatacacca agattataga 15840gcttttattt tgcctataaa aaatggaagc cggataggat accaaaaaag ctttgactta 15900aatttgtaat gcataaaaat gatgatacct aacttattag ccatacttat ctaagcgtac 15960gtcaatttaa atattgtgtt attgattaat aatgatcctt atatatccat attttgacaa 16020ttaaacggta aattagagag aaaagtttga gaaaataatt atagcttacg taatgctata 16080atccaaagtg tctccgcaca agcgtgggac aaaatagtac tttcggagaa gttacaatca 16140acagctaggg agtcttcatt gttcttgaat agaaggatgg aaacaaagtt caccttcttt 16200tattaaagta ttaaggtttg ttattagctc aatatccaat actttctctg ctttttatta 16260cttcgtctgt ttcaaattaa atgatttttt ttttatttta cactattttt aaatttcact 16320tttaccatca tttatgattt atatgtgaat gaaaacatag ttacgtgtga tcttgttttt 16380tttttttttt tttgtnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 16440nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 16500nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 16560nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 16620nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 16680nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 16740nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 16800nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 16860nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 16920nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 16980nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 17040nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 17100nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 17160nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngtacat caaggaaatt tgcattaatg 17220aaaaacggga gtacaaccta atggtaatac aaccaaacta aacagaaaga agaaacaaca 17280gacagtaaga aaacctctat aacgcgtaaa aacaatttaa cataaacact aactagaaaa 17340agtccaggcc gaataacatt tgtcttgtgc gatggggagg gaagatgaag gagagtgaaa 17400atctgttgaa agacacaact ctgacgatct tcatagagag attgtcgtac aactgctagt 17460agatcctgtc cactaggcac gaaatcccaa ggtccttgat gagcctcctg ggattctcct 17520gattcgttga acaaagaagt cactttcgag agtaacttcg gaccctgctg cgagggtctt 17580cgaaaatcag tcacattaga ctgaaacccg aaaaacaagg tggaggtctt gacgtgcccc 17640aaaacaagtt gggggcgtcc cacaagatgc gttttttagg tgtgatgaca catgatgtca 17700tcacgagaaa ttggggcgag ttagtttgat gaactacgcc ccactgacgg atcctaagat 17760ccaaagtgac aatctcgaaa ccagaagacc gacaaacaac agatctgaaa catacaaaca 17820tgaaaataat gaaagcataa actgccaccc gacatataga gctccggcaa acaacaccat 17880caagaacttg caccaaagac ttccttggca tactaaagac actgattcca actaacacta 17940gcgggggacg gggaagggac actcgactac acctaaacct aaccagggga cggggaaggg 18000gaacttagac taaaccttcg taaaaagggg gggatcgggg aggggaaaac cttgaccaag 18060gaagctggtt ttaaaaacca cttagccgag ccaaaaaccg tgggtgggaa gaagaaacag 18120accacaaaca gggggaaccg ggggatggga actcaccgaa caggggaggg ggagaaatcg 18180cacagactcg gggaacgcct aaggactggg ggacgaccaa cgaacgaaag gttggggtgg 18240tgcgaaaaca agggaggggg acgcaccgac gaacaaaaaa accgacgaag aggccgaaaa 18300agcgaaaggc cgacggagat aagattgaaa ggcgacgaaa aaagaaaaag gaacaaaacg 18360aaagaaaaac gaactcgtcg gagacccgcc ggagacctac gcggcgccgg atctccggcg 18420agttctaggg ttagagggtt tgttgtgttt gtttagggag aaggcagagg tttttttttt 18480ttacgtgtga tcttgttaga tttgtcttaa catgtattct ttaatatact ttttttttta 18540taatttttgc aaatgcaaaa ttagagatat atgtcctcta aattttacat tcacatacgt 18600gataaataag agtgctacaa ctaatttgaa acggataaag tatttgaatt gtttttcatt 18660taaaaaagtt cgctatcatt tataatgtta tatatttgcc aatatgttat ctctttctct 18720ctcttaccag agtttagatc cagtagagtt agtaaataat tctaccacgt agagttgaac 18780aaatcatagc cattgatttt caaatcattg gtttatatat tctttcccaa aactcccccc 18840tattttcccc aaaaatcctc cccctcctta tctctttcca taaaatctga gtcgttgatt 18900ttaaaatata aggtttggat tcaactccac tatgtagagt tttcatcaaa ctccaccgaa 18960tccgagcccc tcctaccata gtacttcttg atttccccat atttctttcc tcatcttggt 19020cctcaagcac attttaatat tatgggtatt aaacaataga gaaagtattt acttatagag 19080aaagtatttt caatgattcc ctaaattttt ttttgaaaga aagaaaaggg atttcattaa 19140tatttcgcca aacggcactt acaagtcatt tctgaaaaac ataaaattct aaaagaaata 19200catatcaccc tagaaatgta aacatcgcag atttgactta attttgcctt aataaaaatc 19260ttcatctgaa gcaatgcaat ctgtgagttc gctctggttc ggcatacgat ctgcagatgc 19320ggaaattttg ggatgaacgt actccaatag tcttccataa ttttacagaa gttgtgaaac 19380cctaattctt catgttgaat ctcgaacttc aaccaatgag aataatttct catacctaaa 19440aacaaaagaa ccatactcac aactcccata ggggagaagg agatttccaa aacagaaact 19500aaaaacccca taaaagggtt tgagaaaatc tcataaagag atactaattt attgaacaaa 19560acaagaaaat gaactaaaaa ctgaaaataa aagggaaaaa ggggcttacc atggatgaaa 19620acatccatgg cagcccccta attgatgaag aaggggtaag ggaggctagg gttttagaga 19680gagaaaagga gaggggaggc taggttttaa aaaaaaatat aatgattccc taaatttact 19740tatatatatt taccaagatg acgtgatgtt ttacaaggcc catgattttt acgcgatcat 19800gaaaaacaca gccaatttga atggagcaaa tatctacgcg tcattttaga tatttttgta 19860tgggaaagtt ttttttgacc aatgtaatta ttaagaagca tcggccaccg ggtagataag 19920atgtcactat acatcctttt ccaaacttaa gtatgcctgt tgaacttttg ttgcgtttgc 19980agattcattt gaaattatat ttcctcagat cctctacttg taaaagaatg ttccattatt 20040ttcttagttt acatgatatt tacaatagta tttgtctaca ttttgttcat attacttagt 20100gatcagtgta tacgtcatat attagtttga actttgaaga catttatttt ctatatactt 20160cctttgtctg ctaaattact ttggaaagct ttgttttttt tattaatata agaccctttg 20220gagtttggaa atcactatct aatgaaatat ataattcatc attagaacaa aaatacaaat 20280atcgtactat cacctatcat gttccttttg gatttcgctt cacaaaaata cattttaaaa 20340aaaaataaaa taacaaatgg tagctaacaa cttattactt ttaaaagttt gtgtgcaccc 20400taataagtac tcaaagtagt atgtaacaga gagagtataa tgctaaaata caaactaaat 20460aaacaagaaa gtgtttctca acaataattt gctgcaggaa ttaggaaaca aagtaaataa 20520attgcatgtt tatcatcaat acaatttact ggtagttaat tacaaacttc actcatgata 20580attgaaagag gccactcaat ttcagctagg agttgtttat ttatttattt ttctttcagt 20640taaattttga ctacccacaa aatcttcatc tggacctaat ctgcaatttg tggattttgg 20700atgaaatttc taacctattt aagtagtctt attgtttaaa taacccatgc aattaaatta 20760ggttatatgg gggtgattca tttaccaggc ccaagatttt atctcattct caattattat 20820cgcaacaccc atgaacctaa gccaacatga cttatttacc aggccagcta gagaagaaca 20880aggttgctga ttttcttgtc cgtgattgta gaagaaatgt tagaaatcta aatgttgtta 20940gggatttacc cctcccccct actgagtgta tgaacttatt attgacggat tgttgtaggc 21000ttccaagcca aaactctgat taagttttct tttatgccat tttaaccaaa aaaaaaaaaa 21060aagctaggaa gctagctcag cgcgctctaa ttatttcaca tgtgacatgt tttacactta 21120ttcatacttc tatatgcagg agagggcaat gtaggagcac aataacatcc tgtctaagaa 21180ggtacttgca cttgaccagt ttgtgtaata ttgtaattta atttcttaga ttttggttgc 21240atgctttgat gacgaatgac gattgacgaa tacattttta tgcagatcaa ggagagagga 21300aaaaatctag agtaagtgta atagatgtag tggtagaact agcactagca ctagcactag 21360tagtagccgc caccgccgcc ataaaatcat taagttcctc ctgatgcatc aaatttcatg 21420ctcccacctc caattccttc tttgaacacg gggtagttac ttcttcaact taatttcctc 21480tattcaatat taagttaaga aacagatcac gtgattagtt cgttaatatt gctaattaat 21540aatcatattg ttatatatca tgcattagtg ggtactaagg ataatttggt ggagaagtaa 21600ggaggaatga tcttgacctg acgctagaac cgatatactc atgtcacatg ggatgcttta 21660caacatga 216683829237DNAArtificial SequenceBvFUL genomic DNA showing sites for mutagenesismutation(1)..(1)any nucleotide except amutation(2)..(2)any nucleotide except tmutation(3)..(3)any nucleotide except gmutation(19)..(19)c to tmutation(52)..(52)c to tmisc_feature(1029)..(1924)n is a, c, g, or tmutation(19373)..(19373)c to tmutation(19552)..(19552)c to tmutation(19561)..(19561)c to tmutation(19689)..(19689)c to tmutation(19704)..(19704)c to tmisc_feature(20703)..(20722)n is a, c, g, or tmutation(28161)..(28161)c to tmutation(28185)..(28185)c to tmutation(28191)..(28191)c to tmutation(28194)..(28194)c to tmutation(28447)..(28447)c to tmutation(28450)..(28450)c to tmutation(28465)..(28465)c to tmutation(28468)..(28468)c to tmutation(28507)..(28507)c to tmutation(29166)..(29166)c to tmutation(29196)..(29196)c to tmutation(29217)..(29217)c to tmutation(29226)..(29226)c to t 38bvhgggagag gtagggttta gctcaaaaga attgaaaaca agatcaaccg ttaagtgacc 60ttctccaaac gtcggattgg attgttgaag aaagcgcacg agatctccat tctctgcgat 120gccgatgtag ctctcatcat cttctccact aaaggcaagc tcttcgagta tgcttctgat 180acctggtatg tctaatttta taacttcttc ttttgtacat caataatttt atcatcgact 240caactaaaag cttaagcaga tggttagggt tctattatta ttgaattacc tcaaatttgt 300catcgactca actaaaagta gagtatattt catgtagatc aggtgctttt tttgaatata 360ttgtcagttt tagaactaca aaatgttgaa cacaagtatt tatacgcacg ctgacatgtg 420aattttttaa ttgacaactt tctaaattaa tactctaaat tactaatatg aagaacgtaa 480tttattattt atcactttca gacaaaggca tgtttgtttt ttctattatt tttcccatga 540aaattctcac caatatccga ttctgtatgt taattttagt aatttctaat tttgatgact 600taataaattg taaaaaagta taaaataaac aaatatccaa aacatctttg ttttcaagag 660aaatatctta aaaacttttg ttttttaaga gaaatatctt aaaacacttt ttatcatact 720actatgatga tgtataaatc tattcaaaaa aaaaaaaaaa tgatgatgta taaataattt 780aaagagttaa gtttattaga aattatagat atttatagag ctgagtaata aaataatact 840ctacagatta tatgtagctg atgtagtgtg tctgctcctg taagatttcc tttttatctc 900caaaaaaatt gcattgatat tcgagccttg ccgaccccct tttcctcttc aaccatttga 960taagatccta tgcactgagt aatctagtat tatatgttag atgttatata ttaataagct 1020aaaattgtnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1080nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1140nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1200nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1260nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1320nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1380nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1440nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1500nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1560nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1620nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1680nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1740nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1800nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1860nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1920nnnngatgat attatgtatc attattatca gtcatttatt attgatagat aatgttatta 1980tttccataga tcatatataa tcttaccata tttactccct atgattatat atttctatct 2040attaacatag taatagtgat atactaacaa cgagtatctt tcaagtaaat aaatcatata 2100tatagggtta gaaggtaaaa aagacaatat tttatgagac tttatttaca cccttcgtct 2160cataaactcc tttctatttt ttgtgttcac tcgttttcta aagttttttc tacttctttt 2220actttttatt ggataaatac tttttccgtc tgtatgtgat ctacttatat tagtatttat 2280agatagatta tactcttaaa gtattacatt cacaaaatca tgtgaattat taattaaagt 2340aaaattatga gtatggtatt ataacttaaa cgaagtagtt gtattgttta aaccaataag 2400tataataact tataaaactt aaaagttgat tctacactta tcatgcactt gtgttttgat 2460gggttaaaaa ttagctagta cataagtaac ataacataaa cttatctctg tatagtatgt 2520tgaattatta ctttatattt gaaaagaaca aagacacaat aagtccaaaa gatccgatct 2580tttgattatc aactatgtaa gtgtctttcc taaatgatca atcaccttaa ttagtatact 2640aaacaggaca attatgacat ataacacttt tctatttgta caagttaatt aacccattga 2700cattcattca gttagcctac atttttcaat aggtagtcat catcattctt tttttaacca 2760atttttttac aataaccatt acctaccaac aacattttca aataatgaac tgaatgatgg 2820attccgtcaa ggaaattgtt cgtggacagt ttgtttacct cccaaatttc ctttaatctc 2880atgctttctc catcatccaa acaatctaat accaatcgtt ttctctaatt gcataaaccc 2940taattgttga atcctttaat cttgcctttt cacattgccc aattccttta gtatgatatt 3000ttttattcga taatccctga taagtaaatt cactaatcta atttcagatg gattgttgta 3060gagattgggg aattgaagat tttttgcctt ctttgttctt gtttacaatg aaggatgaag 3120tttcatggaa tattgaagag aaatttgaga aaagaaagaa agtgtgggct ttactgacga 3180caaaaactgt cattttggtg gtttttttca ctaaggcatg tttggcacta gcgtttaagg 3240tagcggttag cgatttgaca agatcaaaac gctacttaag aaaatgatga gtgtttggta 3300agatagtggt tgttgtagca ggtagcaatt agagtagatt atgagtagcg gttgtggaat 3360gttactacaa gtaacgtttg agatttagag gtagcagtcc agcaaagaaa cattgtataa 3420tagcataagg tagaattaat taaccaatgt ttttttattt ctttttcctt ttattgttta 3480ttaataattt tattttatgc caattcaatg tttattttac aatagcaaaa atgtaattta 3540aatatttatt ttaaacataa taaattttat aagtatttta gtaaaattgg taatataaat 3600ttttcgaggg ttgaatattt tcttagatgt atacatttct ttgaatagtt aaaaaatcat 3660atctcctttt tgtgtaactt ctttgaaaaa taaatcttga atttaactat tgaacgaaca 3720tatgaaatta ttgtagttca tttcatatta taataaatgt taggtattgt cttttacaat 3780ttcaactact tttggcaaac aattctgatt aaacagctat tttaatcgct gatcgttgac 3840agcaaccgct aacagctact agaaccgcta cttttgccaa acatgcctaa accaagtaat 3900atcggggatt ctttattata taaaaagtta ataatgtgat tatttgaaaa aaaggttgac 3960tttatatgtt gtttaaagaa aaaaaaacat ttattaatag aaaatatact atataggtct 4020ctatataccc agggcgaaat gaatgtgtat cttttcaaat gagtatgcgt acatgttctg 4080taaatgcata tttcatatga gcataatgtt tttactatta ttatgcacat ttgtgtttta 4140atttttcaaa tgagtatgta aggaaaacat gtattcttgg catgtcagtg ttagtgattt 4200ttgttgttat ataaatgttt tcgtgatttg tgaatgtggt acaaacattc atgtgccatg 4260gcgtttagca aaacttttct agctcacatg atgcttcaag ctaattgcaa tgaactaata 4320taagggagag gacattttac gaattagttt tacattgata gtagtttgaa gaagatagtt 4380taggagatag tttgttggaa tagaagctat gtgttagaat tagttatagt catcaatttt 4440tgaataagac tcattattat ttcaatcctt ctacctttta attactagtc caactctcac 4500tctttggtat taaattacac cattcctacg gactatctaa caactctaac cacggcccat 4560actttttctt cttaacataa aataatatta cgcttactaa ctactaactc ctatgacatc 4620taccttttca ataaaataac aattgataac tatataacaa cttataatct aaaagtaagt 4680gtcttgataa gtgtagagta ttgtgggacg aagggagtaa ttcatagcaa atactatcat 4740agcaatacaa tactaggaga ctaagatgtg agttttgaac ttcaaaaaaa aatatacgcg 4800acatagttca ccggaagaac tgcagcacaa caaatgcaaa tggggattaa atgaggagtt 4860cacctacatc acacacaaga gcgattgagg attttcagat ctggaagaag agcgaaaaat

4920caggcgagag cttcaacttt cggagtttaa tggaagagct aatgatgatt gaattattca 4980gttggattta tcttttgtaa gatgagggga tgaagttgaa gattgccata ataacccttg 5040agggcgacac aatggtggtg ggaatggaaa tatggaccac gaccgattta tgagtggaaa 5100gaattgaagt ctctgataca atgacgtttt ggtatatcat cggtgctttc atggctgtca 5160gatctaccga aaggaaaagt agtggtgaaa acaaatgtcg ttcaaaccaa ccacctttga 5220ctttcaatat tgaagtgaca aggaaaactg gcaccttagc tagtgacttg gtggagtctt 5280tgcgtaaacc ggtggaaaag aagcttatct ttgagtttta gtgtggtctt ggtgcttgtt 5340taagtggctt gactatttag gaggaaaaaa atcaattgtg aacaattggt gagtaaccta 5400aaaatcaatt gtgaacaatt ggtgagtaac ctaaaaatca attgtgaaca attggtgagt 5460aacctttaaa ccagactcaa gagtgggaaa gaggcggtta gacaagtaga tttagaggaa 5520gaggaggtag aaaatagatt agaatttttt ttgggagttt tcaaatgtag tggtatcggc 5580tcataacaag gtggtcaaat ggatggagag cttggtcgtt tgagtagtct catggtggtg 5640attataacta cgttggcgat aaacaagttc gctttaccaa gaagttgatt ggtggaagag 5700agattttcat aaagaggggc tcgtatagca acagctttgt gtggtctcag tttcattgtc 5760ggcaaagccg tgggtggtaa atatggtgat tttggagatt gttatgatgg agggagttat 5820ggtggtttca tgagacatgg atgctatggt ggtgctgtga tcggtggaga agaggagctc 5880gtgaaaggtc actctgatgg agcgggtgta atggtggtct aggttttttc ggcatcggag 5940gttgcgtatc tcgtgaaggt tcacatttgt acaccggtga atactatggt ggtgaactag 6000gatgacagtc aaggtgacca tagtagaaaa aaaaaacata aaccatgtag cttagatgat 6060ttgaaaaaaa atcatgtttt tggagaagaa tcttaaatat tatgacagag gcaaacttgt 6120cattgaccaa atagatgaca tagcaattgc acgtgtctcg ttaaaatttg aaaccataaa 6180aaattcaaat tgcacttcat atgcctttct tttggttgaa aacttcatat accctaatgc 6240gtcaatatgg ttctttttcc aaaaaaaaaa gtaaattatt tcggcgttag taaaagcagg 6300tccacctcca taatccattt tattaagcca actcctctac cctacttttg caacctatca 6360tttcttattt tctaaaatca tatcaaaaaa acaagtgtga acccaaaact aactatatta 6420tacctaagtc taatttcttc atccaatgtg ttcaacccca tttttcaacc cttccactca 6480taaacccatc ttctttcact cctaaaactt tcagctcacg ctcgtatcac ctctttactc 6540acatatcagc ccaaacgatt tcttattgaa tccactaaat tatgtatatc gattttttca 6600ccaagtactc cgagttttca aaaaatttac ctggtacccc caagttttca aactacacgg 6660gataccccta agtttcaaac taatacactc agataccctt aatgactaac gacattaatc 6720gccgttagtc attaacctta attttctaga tttcaaccta attaaccact aaccctaacc 6780ccaaccctaa ccctaataat aaccctaaac ctaaccctaa ccacccctcc ccaaccctcc 6840caccacccct gccccccatc ctccactcct gcgcagccag caggcccccc acccatttga 6900ttttaaggaa gaaacacgta tcagggaagg gggagaactt agctctgaca gcggcaacgg 6960accaccgacg agtctggact gtaggacggc agcgtcaagg cgcgagccgg ttgggctact 7020gcagttcaaa gcaaacaggg gaggggaatc gagccgagaa gagagctaag gaaagagggg 7080tgatgggcgt tgcggagatg gtgcgcatag tggtggtggt gtgggggagc tgtggtgggg 7140gagaaatcga atgggtgggg gagagagggt ggggggctgc ggtggggttg gggctggagg 7200gggtagggtg ggtgggtgga ggggtggtgg ttgggtggta gtgggacaac tctcaaacat 7260gattctttct caaacatgat tctttctcat atcctttttt ggatttcctt aaaaaaatcc 7320atatccaaat aacgttgacc ggtgagggac aactctttaa cactattctt tctcaaatct 7380tatgaaatct tcacatttaa ctcgatctcc ctttcaatga acctaaagat atatattaat 7440agagttgcaa attcctaaac tctagaaaat tcaaatacaa cataagagtc ctagattctt 7500ccacaagatg tattatatct ttcaaagttt cccagataaa attagtaatt aggaaactcc 7560ttaacaagga tacttaaagt tttatctaaa tcttgcataa attgaaatcc aaccataatt 7620atgaataaat aatcataaag aatcctaaca taaataacta gaaaataaga taataaagaa 7680gcaacaaaag aatctcataa ccaccatttg aatcccgcat gagaacccaa atagttgttg 7740ttccttataa aaacccacca cctttcttcg ggtattatga cggtattgga ctatagtatg 7800agacgagatc tcttaatcac caatcaacta ttgtaaactt gtgagcctga ataatttatt 7860tgagatacaa ttctaaggtt gtttatgaac gtgtttggta aaattgttat tgataactcg 7920ttcgtggaaa ataaatgcaa aagtcaacat gccaaaaaaa gtgctaaaat caactttcgg 7980ctttgcttga aatgttaagt tttaagctat ccaagagcca ttagtcaaaa tctattgaaa 8040gcgtactcaa aaaccattta tcaaacaccc ctacaaatcc ctttagaaaa caataggagt 8100tgtacaatat aagtattgag ttataaagtt gatcaagtga tttaggaggt tgttccaaat 8160caatctacaa gagtttgtat acttataccc cttcgttttt ttaattgtta cacttaggcc 8220ttgtttgaca aatagagttt agcggttaga gtttagattt tgctgttaga gttttaactt 8280tttgttaaat agatttgact gctgatttga caacttcttc ttataaatgt gtttggtaat 8340tattaacaga ttgctaaaag cttattaccc tttatttatg tgaaatgaca tgtatagaca 8400ttttaatcca catgggtatt attattatta ttattcgagg catacaagtc attgaatata 8460tttttaccta atcctctaaa gaaaaagctc ctattaggag ctttttcatt tcgagagttt 8520ttattccaga actctcttca aaactctttt ttaccaaaga ataggagctt tttcatttca 8580agagttttta ctccagaact ctctttaaaa ctctttttta ccaaacaccc cttttagagt 8640ttttgactag tcaaaactct aaaagtggtc caaatttctc ttttaactcc aaaactctaa 8700ttgccaaaca cccccttaca cttttcacgc ataccaatgc aacactttga cgattaacat 8760ctccagtttt ttatttgtaa aaattataaa gagtgcatat taataagtag ggctgttcaa 8820agtgcggtct ggaccgcacc aaaccgcaac ccaaaccgtt gtttcgcggt ttggtttggt 8880ttgcggtttt aaaattgcgg tttgggttat gatttcaagc aaaccgcggt ttgcggtttg 8940ggttgggttt ttatttttgt aaaccaaaac cgcaccgcaa accgcaatgt tacatttttt 9000ttaaaaaaat aaattaaata catttatgaa ggtgacatac aattataaaa ttgaaaaaag 9060aagtttgagg taaaaaactt taacacttat gataaatcat tatatatgtt taattatgaa 9120ttcagcttca tatctatttg gactcttatt aacaattttc ttttaatctt aggaaacaaa 9180agtaatgtcg cggaggaaat aatggttaaa ccgcaaccca aaccgcacca aaccgttttg 9240cgcggtttgg gttgggttgg tttgggaaaa agtgcggtgc ggtttgggtt ggaaaatttt 9300caaaccgtat atttgcggtt tgggttgggt tacatcccaa accgcacaaa cccaaaccgc 9360gaacacccct attaataaga tacatattaa ttcgaatttg acaagatcca catgactatg 9420tttttattcg cgtataaacc acaaaagaag gttcaagtaa aatttgtgta tggtgtaaca 9480tgtcaatcaa agaacggagg taatatttgt caagacactt tagtcacttc taaattccta 9540taaacaaaga aatatggaag aaaactggtg atgaaaattg aaaaggtggg tataataaga 9600gagacacaat tctaaaataa gaaaatatta ataataaaat aataagttac gataggcctc 9660atgtttgaaa acggaaaaaa taaggagata gttcgtgtaa aaaggaggga gtaaagggta 9720atgcatactt tgtattgcaa gcttagtttt aaaaggcata agacgcaaag cgcatcgagg 9780cacaagacga aggcgcatgc atctcgtagt tgaggcgtgt aatgatttta cttcacaacc 9840acctgagcaa cccaatacag aacgaccaca agaaaaatag aaagaaagga aatgattttg 9900attgatcagc agaaaataca gagcattcga gaggctcagt ctctcccaag gactacaaga 9960tactactaaa tttcacaccc ccttcagtcc ccttacaccc ttatttatac tacttctgct 10020ctcctatttt aacggctact gacattctct gagctggcct gctattcctc tttttgtgct 10080gacatttctg aatattctgt ggtagtggct ccattctcac atttggacag gtttacccct 10140ccatttcttt cgtgcatacg tcagccacgg tttggggatt gaattcatta cattaccctg 10200cccccaaaga ctcaccttgt cctcaaggtg gaaggaaggg aaacgttctt gcaccaaatc 10260tgcatcttcc cacgtggctt caaaaggtgc taagtccttc catttcagta gcacttcagt 10320ctgcgtatgc ctccctctct gtgtctgacg tacgtccaat aattcttcag gttccacaac 10380tagttccaag tctgctgcta gttgagttgg tacagtggtt gctgcctggg catctccaat 10440tgctcgtttg agctgggata cgtgaaatat aggatgtatt ttactggtgc ctggtaactg 10500gagtttatag gcgaccttgc ccaccttttg cagaacaggg aatgggccat agaagcgggc 10560tgccagcttc tcaaatggtc gcttggccaa ggattgttga cggtatggct ggagctttaa 10620gtaaaccaga tcccccactt caaaggactc atcgcgcctt cttgtgtcag cataggcttt 10680catcttttgc tgggagcgta gtagatgaaa gcgtaaatca tcgaggatgg catcccgttc 10740ttgcaacact tcctctaagt tatctactgg cgtttgccct ctgcctactc gccacaagtg 10800tgtgggtcac gcccgtacaa caccctgaat ggagtcagct tagtagacat gtggggagag 10860gtgttgtgtg agtattcagc ccaagggagc cactttgccc aagtcttcgg gtgccccgcc 10920acgaaacatc tcagatatgt ctcaagtcct ttgttcacaa tctcagtttg tccatcggtt 10980tgcgggtgat aggcggtgct tctccttagt gttgtccctt ggagtcgaaa caactctttc 11040caaaaagtac tcagaaaaat tcgatcccta tccgaaacga ttgatgccgg aaacccatga 11100agttttacaa cctccctgat gaaagcttca gccacttgtg agagcactaa aaggatgacg 11160aagcccaatg aaatgcgcat atttcgataa acggtccacc actactaaga tcgtgtccac 11220ccccttggac aagggcaatc cttctatgaa atcaagagtg atatcctccc aaacctgagc 11280tggaatggct aagggctgca gtaggcctgc tggcttttgt tgagagctct tatgctgttg 11340acaaatgcta catcgctgca cgtacaatgc cacgtgcttc ctcataccta tccaatacca 11400ctcagccgcc aacctaaggt acgtttttac ttcacctgca tgtcctcctt ctggggaatc 11460atggtaagct atcatcaact taggaatgat gacggaagtg ttgggaatta ccattcgccc 11520cttataccgc agcttgccat cctccaccgt gaaccccaca agtggtttat ctccctgcgc 11580cacttcttcc ctgagccttt taaggaacca atcctcctct acctcttttt ggagctctgc 11640ccagtccact ccttgggttg tgattatggt ccctagctcc atttcaccta cagtttttct 11700agaaagcgca tccgcaacct tgttggttgc ccccggtttg tagtgtattt caaagtcaaa 11760cccaattaat ttgcttaccc atttctgaaa atcagccccc acttcccgtt gttgtgtgat 11820gaaacgcaaa ctttgttgat ccgtatggat cacaaatctt ctccccaaaa ggtaatgttt 11880ccatttctgg accgcaaggc atatggcaat taattccttc tcataaacgg acttgtgttg 11940tgctctcggt ccaaggagct tgctgtagaa tgcaatgggc ctgccctctt gcattaggac 12000tgcccccacc ccatacccag acgcatccgt ctcaactacg aaaggcttat ggaaatcggg 12060catagcaaga accggtggct gggtcatagc ttcctttaag tgagagaaag ctgaagtagc 12120tttttcggac cagccaaagg agtccttacg caattgctcg gtaaggggct gggcaatttg 12180cgcgtattgc ctgataaact tgcgataata cccggtcagc cctaaaaatc ctcgaagctc 12240cctcaaattc ttgggaactt cccactccac catggccctt atcttctcca tgtctactgc 12300caccccatgc tgcgaaattt acatgcccca agtaggccac tgtcttcctc cccaagtcac 12360atttcttctg gttagcgaac agtttgtgca atgctaacag ctgcaacacc aatcccatgt 12420gtcgtgcgtg gtcctctttg gtcttactgt agaccagaat gtcgtcgaag aagaccagca 12480caaacttcct cagatatgga cggaaaacgt tattcatgag tgactgaaaa gttgctgggg 12540cattggtgag cccaaagggc attacgagaa attcgtaatg tccttcatgg gtgcgaaaag 12600cagtcttatg ggtatcctcc gggcgaacta aaatttgatg gtatccggcc ttaaggtcga 12660gtttagagaa gatggtagcg ccatgtaact cgtctagtag ctcatcaatg accggtatcg 12720gatacttatc cggaaccgtc tccttgttca aagcccgata atcgacacaa aacctccaag 12780aaccatcttt tttcttcacc aataatacgg ggcttgaaaa tggactagtt gagggcttga 12840tgatgcctgc ctccagcatc tctcggatga gtctctcgat ttcgtctttt tgaaattgag 12900ggtaacggta tggcctaacc cccaccggat tactgccttc cttcaacgtg attgcatgct 12960catgccccct ctttggtggc aggcccaccg gagtatcaaa aacttccgca aactgactaa 13020ttaccttctg taaaaattcg ggtacttctt gtgcctcctt caactccgct tctccccttt 13080tcccatcatc ctcaatctgg ttgagctcca agagaaaacc ccctttttct tttcggattg 13140cctttatcat ggctctaagt gagattttag atcttgctaa ggaagggtcc cctctcaatg 13200tcaccactct gccctccact tcaaactgca taacctgagt tttccagttg gtaatcactg 13260accccaattt ctcgagccac tgcactccta atattaaatc tgagttaccc aggccgagag 13320gtaaaaaatc ctctgttact tcgatttccc ccagctttaa agtcacccct tgacacaccc 13380cagtaccatg gacagcttca ccattcccta aagacactcc aaatccccct gcatctgaga 13440tgaccaactc aagttcctca acagttaaca aggaaataaa attgtgagtg gcacccgggt 13500caatcatgac caccacctct cttcctttaa tttttctagt gattttcatc gttttaggac 13560tcatcaaacc aatcacagag ttgagagata cctcagtagg aagttccggt ggtggttcgg 13620acggtggtgc acgagctgcg tcgctcacct cttcggtttc ttcctcctcg tcatgcatca 13680gaatcacgct gatctctttt ctccggcaga tgtggccggc ggtccactta tcgtcacatt 13740tatagcacaa cccttttgct ctcttctctt gatattcttt ctcggaaagt cgcttgaact 13800ctacagattt ttttccttgc cccccagcaa ttggatacgt gttcaaggtg ttggaatttc 13860cccctgggtt ttgggcccac attttgctgg caggtgggtt gaggctggtc gttggattga 13920aggaagcccc tacactcctt gtcattcccc ctctgttata aatcgagtaa ggcccattct 13980tagttggccc acttctttta taacccacaa tcctattcct ttcctcaatt cggcctgcta 14040gttccattgc ttgctctagg tccataggat tgagtaacct gacctccact ttgatatcct 14100cttccagccc attaatgaac tgacccatga gtatttcttc tggtactcta ctcaacggtg 14160ccgccttctc aataaaagtg cgtcgatact catccaccgt ggtggtttgc tttgtggcca 14220accaccgttc ccacaatgaa ccatagtggg ttggtcgaaa ctgacggagg aggtactcct 14280tcagatctgc ccaccacctt atcggccgcc ttttattctc ccactggtac cacctgaggg 14340catccccctc tatagacaca accgccgcct ccagggcttc actgctactc aggccataaa 14400acgaaaaata tcgctcggct ctaaggatcc acccatccgg atcggaccca tggaaaatgg 14460gcatctctaa ctttcgatat ttccagttcc ccccggaagt cgaacctcct ggccctccac 14520ctgagccgcc atccccatac gatcggcccc cgagctggaa acctccatat tcgtcccctt 14580ctcggccccc cagatttatc aggtcaggag ccctccgttc cggcggtcgg gggtgtccag 14640tgacggtttc gggtgtctcc cgcgagggtt gtggcaaggt tgctcggatt tcaacctgaa 14700atttcctctg ttcagcacgc aacccttgaa tcgtcccatc ctgtgtctcc cttgaccggt 14760taatccggtc ctccagccta acggccaaga cctccatctc ctcgcgactc ttcctcgccg 14820cctcattctg gccttccaag atttgagcgg tcaacgattc ccccatggca ttaatggcag 14880attccaccgc cctggacacc atggtggcca ctgacccttc gagggctgcc attctttctt 14940ctaatgaatc caccctctgc acttcgtttc ttggtgccat ggatcgatgc tctgatacca 15000agtgtaatga ttttacttac ttcacaacca cctgagcaac ccaatacaga acgaccacaa 15060gaaaaataga aagaaaggaa atgattttga ttgatcagca gaaaatacag agcattcgag 15120aggctcagtc tctcccaagg actacaagat actactaaat ttcacacccc cttcagtccc 15180cttacaccct tatttatact acttctgctc tcctatttta acggctactg acattctctg 15240agctggcctg ctattcctct ttttgtgctg acatttctga atattctgtg gtagtggctc 15300cattctcaca tttggacagg tttacccctc catttctttc gtgcatacgt cagccacggt 15360ttggggattg aattcattac aaggcgcacc tttggtacca agagacttag acatcaaata 15420aagaagcatt gtaatactta gaataaacat tgtttttata ttctaaaaca catattttct 15480ctaagacacc tagtaatctc atagtggatg tcatctagtt taggtggtaa ggttttattg 15540agttggtact caagacctca gacttagagg tggcaaacgg atcatacggg tcgggtgaaa 15600atgagtcggg tcataatcgg gtcacctttg tgtccaggtt acggtcaggt cgagttcgtt 15660cgggtacgag ttcatattga gtccatgagg tttcatgtca tatcgggtcg ggttagattg 15720gatttacaat ttcgcaaata aataaaacgc atataatact aaagagagta aattaaataa 15780ttaacggaca ctagctaaat catatattag tattttatga tgtattttcc ttaaatttat 15840ttaaaaaata actaatatga caatttttcg ggccgggttc gggttgtggt catcattatc 15900gggtcaattt agtatcgggt aggctcgggt tcatgtcata ttcgggtcta ttttaattcg 15960agtcgggtta tttcggattt aagctctatt tcgggtcagt attttcgatg aagaacgggt 16020ttcggatcgg gtcaccggat acggatctat tttgccgagt cagactgctt ttcaaaccta 16080ataatctcag tttttccacc tattcagatt tgcctatgat ctctatttag ataaatgagt 16140acaatattgt gtctatccat gaaaatgaat atctcacaat gtaaaaggat atctctaaat 16200ttcactaatc actctatctg ttttgaataa taatattcta ttttattgca tgtagtaaag 16260atcgagtatt tagtgagatt tggagaaaag aggaggctag agagaaacta ggatttagag 16320aggagaaggg ggctctgtaa cacatacaag atagatactc ttttacacta acttttcaag 16380atactcaaca tataaaatca gcatcatctt ccaaacaaca actttaagcc acccatgaat 16440cttaattaga taataaaaca taatcgtgaa tcatctatcc tttgtttggg gggatcctaa 16500agcaattgag gaaaagcttt gatgcaaata tcaattgtgt aaaaaagcaa gtattcgttc 16560gtgatgttgc tatactaggt tattttttgg atccaaaagt cattcctact agaatcattt 16620aggaaaattg tcagtatgaa ttttaaattc aggttataac caaagataat tgaaaattgt 16680caaacttttc aaataattcc gaaataaaca tgtttgtaac atggataaac ttttcattgc 16740ttttcaaata attccaaaat aaacatgtta taacatagat aaccttttca gataattcca 16800aaataaacat gttgtatcat ggataaactt ttcattgttt ctagtcactt aaaattctaa 16860aaaaatcttt cctccctact gttactctct ctagcaccaa atctatcaca tgagaaggca 16920gaggttttca aaataaaccg ttacttaatt tggtacttat ttcttgatcg gtgttcatat 16980catatgagtt cctactctat atctctctac tcttctaaat ccttgtgtca cttcctgtgt 17040ttcataaata aaaaggagga agtattagtt ttgaaacgaa aggagtatgg tgcatacatt 17100gatagaaaaa agaagttatt tgtccttatt tcactcatat aacaacacca aattctgtat 17160tgttatcaca aaataaaact tggattatct ttgtttcata gcccaaattt agaattagtt 17220tgtcagattt ccaatcatct aattacaata ttagagctag acctaggaca aaaggtgggt 17280ttggctactt ggtaatagct atgtctagtg ctaggatatg tcattgtcgt agaaccatgt 17340tatggacatc ttaagaaaca aggttaacct aattggttgg agatcctact ttcactttta 17400taataaagtt tcgattcttg cctatttgta aagtagaatt cctaaatttc ccttcactga 17460tatttatctt aacataaaaa aatgttataa acattgggat tgtatataag tcaaaataaa 17520ttgacaatct tggtaacaac taagttaaca ttaattttat aagtaaatga ttaatcccaa 17580tataatctct tatttagtaa atgagacaaa cttgtacacc ttcgtgttag actcgttaat 17640gttcgctaac aattcattca gtagtcaaca gcattttaaa tttgaaataa gtgttcttgt 17700ggtttttgag agatcaagca agaaaacatg tctctcccct ttgaccaact aattgggatt 17760aagaatacta gttttaagat tttaagaatg agttatagtc tttcttagac cgctacaatc 17820cccttgttga tatgaaccag atatattttg tgttcaaata gtagatcaat gcattgttga 17880taatcctttg ttaatgtact tgttgatctt attttgtact tttggtagat gcgctatact 17940ttctttcgat tgctcatttt gaactcttaa ctacatatgt tagtttaagt agatgattta 18000gaattgctat ttcaatcttc aataagcaat ttaagttgtc aaaccttgtt tcacatcatt 18060agggtgaaag ttatttggat aaagacctat atctaattca atccaaagca aattagtaat 18120gcggattgga ctcaaactat gtttagattg gattcgaatt gagtttcttt tctttttctt 18180aaaaaaattg gattttccga tcgaattgag ggtgattaga tccaaaataa ccgaatagta 18240gataggattt gtgttgtata ttagaattgg gcttaaggat ttccatttta acaaaaaaac 18300caaatggtcc gactatcaaa aactataatt tgatagtcat gcctatcgaa aactttattt 18360tcattctcgc acctaattat gggcttgtat aaattagttc tactatcgaa aactaatttt 18420gatgctcgtg cctaatttaa attttcgaaa aaatgaagtt aagaaaattg gatatttcgg 18480attggatcca atatatcttg tgaattatta atttggatta gtttggactc aaattcttat 18540tggattggat tcaaattaaa agattaaaat tcaaattttg ttcgaatcaa attggagtag 18600gcttaagttt aaatcataca ccgaactttc accactaccc atcatgctta agcttctaat 18660gtaagagagt gtttgggagt tgagctcgaa caactaaatt tctaaaagaa ccaagttcaa 18720acaagaaatc taaaagctcg attaaacttg agtcaagctc aaacacctat attccttatt 18780ggagcttgac tgaagattga acacttattc cttattaagc tttacgctaa aacattgctc 18840gactcaactc ttctacatcc ctatagttca aaagaaatag ttgtgggctg tggtgctctt 18900gtagaccaac gcactagttt aacaaagcta agtgcctgac tgcaattcca tacacattac 18960gatcaccatg acctagtttc agctcacact ttggaagtct aatttgaact tgttctctac 19020ctccaattca ttgtggggta ggaggcgata gttaagggat caaaatctta tgatataact 19080tgcataggct atgccactat ataatgcgtc ttgtgtccca tattagttta atcaaattga 19140aatgttttac catttatatc ttcaattatg tatggatact aatatttgat ttgacgtttg 19200atatgatatt aaatgtggac tgttattctt gatgtgcttg agaagctttt ttggggccag 19260ttagaaacta tattcctttt atggtcctaa ctaggttgtt gttggtgtgt tcccaaataa 19320cagcatggaa aggatactcg agcgctatga aagacactca tatgcagaga gataactgac 19380tgctccagat cctggatccc atgtaatcca gctaggcaac tatcttttct aagcatttaa 19440atcgttgaga tttcaatttt aaatgtgttt taactgataa ttcatgcatt atatgcttag 19500gtaagtttga ctctggaaca cgcaaaactt aaggctaggc tggacattct ttagaaaaat 19560taaaggtaat aagatccaga ccaaatataa tttgtataat aaccacctta tgaggaaaat 19620ttaagatcct tgataatttc aggcattaca tgggagaaga acttgatacc ttgagtctca 19680aggagcttta gaatttagag cattaaattg acagtgctct taaacacatc aggtcaaaga 19740aggtagtttc acagttgcat tagatcatct tatggatcaa ttggatcact tgtttgtatt 19800ttagcgttgc tcaacacggt cgtctaatat agtgtgcaaa acgacctaca gggcaacacc 19860ttttataggg ctcgaaaata cgaaaaatta aatgtttgtt ttagtcatat tgttcaaacc 19920caagctttat cttgtcaaaa atattttata atgattattt tttagaatac attatttaca

19980tttttgcaat ttatgcataa tacttctaag gtccaacttt ataattgaaa tagaagtcct 20040taaattttaa agacgacctt gaggaaacct aatttcttct catatataat taaatcaatt 20100attctacaag ttagtagaac aaatactaca ataacaacaa tattgaagcc ctaatctcag 20160taggattgga ttgattgtat gaagtcttat tagtggccgt taaatgtttc ttgtaggtca 20220agatgacatg gctcatatag taaggttact tgactaaaag acgaggattt gtttcgactt 20280agattttaac aagtttccct catttgttaa cacctaagcg tactaaatca aattctaggt 20340tttactcact caaatttccg atttaggaag ggcttgagga tagttgtatt atcgtaactg 20400actaatcaaa ggagcctctc ttagatcagg tttcacttgc caattctaac aacttgtttg 20460gtaaaaggaa tttggaatga aaagaaagga attgaaaaga aacattctac ttttcaatgt 20520ttcattcaaa aataacattt taagtgatag gaaatggaaa gaagtgaaac gaaagcctct 20580ttacaaaatt atcatttttc tacccccccc cccccccaaa aaaaaaaaaa aaaataagta 20640gtaagtagta gaagaagaaa taaataacta acaagagtag taagttttta cgttttcttt 20700ctnnnnnnnn nnnnnnnnnn nnattcaaat cagaactgaa tagtcataac cggaagatta 20760gtttctctct agcgtgacta gggtttgagt aaaaagagaa aacttaaatc aaacatggga 20820tattaaggtt ttttttcctt tcttcagttc ttttctcttc ccaatccttt cctaaaaatg 20880aaccaaacag gctgcaaggt tttcacttgc ttaacacaag atttattttt aaaaataatt 20940acactccaaa cttttaagct taaaaccaat tttaattcaa atcagaactg aatagtcata 21000actggaagat tagtttctct ctagcgtgac tagggtttga gtgaaaaagt ctagggtttc 21060atgtcattct tcttgcttcg agtcccttct tgggattgtt gttagccatt atggctaccg 21120aaatcgttat taaatgtcta aatcttagaa ttactgctga agaaaacaac ttggtgtttc 21180tcgaagatgt tgatgataac tcgcagcacc atacgctcgc actggcgatt gttggaaagg 21240ttctttcgtc aagaccatac aatttcgagg cacttaaatg aaccttaaac tagatatggg 21300tgatatccaa aggagcccta cttcacccta ttgaaaacgg actttttgtg gtacaatttg 21360cgacaattaa ggaccgatct aaggttctag tcagcagacc atggaccttc gatataaacc 21420ttgttctctt agatgctatt gaagggggta ctcaatcttg acccattgcc cgttttggac 21480tcgcttgtat aaccttccta tggactgccg atatgagaag ttcatcaaaa actattgttg 21540gtgtattggg ggaggtattg gaagttgatt ttgacaggat tgtttgggat aaatctgcaa 21600gagtaaaggt gaagattgac attacaaaat cgttttgtcg tgtgcagatg atcaagacta 21660acaggggtga ggctgtgatg atcaatgtta agtatgaaag acttcctaca atttgttatg 21720tgtggaattc tggccatatt gaaagagatt gtgtgaagac ccaggaagaa gagaaacaag 21780tggagagaca atagggggtc ttggaggcct ctccgcgtag gggacgatta aagatggtga 21840aagagtcgaa agccttcctt cagtgtgctc gtacactcca ctttaataac aaggaagaag 21900taaggggtga ggaaccacgg gattatgtgg agccgagggg ttattgtcgg ctatcttagg 21960gggtaaaact ttggtggtcc aggagatagt ggacggctct aaggatgcca tcgaggaagt 22020tcgtgctgaa ggtgcaccac tctagccccc ttgtaccctt tgggtaatgc catgctacct 22080tttacttttg ctgttgggag tgctaatcct actccctccc accgaaaagt taaaattaaa 22140aacaaggcaa gggttcaggg tgttttgaac caagttaatg ttgtgggtgt tggggggttg 22200gctaataatg ggggttgtga gaaaaggata ttccccaacc cgatggtgtt agaaaaagaa 22260aaggggttca atgaagaggg tttaagatag caaaacgaga ggattgtatg taacctatca 22320gtagggaggt aactattgag gtggaggtgg gcgagaccca accccgcccg acattatgaa 22380tatcctatgt tgcaactgtt ggggattggg caacccccgg gaagttcgga tgcttcgtag 22440gtggagcaat agtgctacac tgagttcggt ttttatttct aaaactatga ttagtggtcg 22500tgatgtggaa agggtgcaaa gcgggtaggg ttttgattgg gcaattgggg tggatagcgt 22560tggaacttca agagtttggt gcatttattg gaaagctggg gaagtggact ttactctagt 22620ctctctatca agtcatcata tttgtgggaa tgtgaagctt gttgatggga aggtatgatg 22680cttagtgagt atttatggtt gggcggatac aattcaaaag tataaaacat gggagcttat 22740gcaatccttt cactcatatc atgggccgat attgtttggt tgggacttca atgagatttt 22800gacaatcgga gaaattgaag gagggtccga aactcaatga agtaacatgc ataattttct 22860agaaacttta gatgacatga agcttaggga ccttggctat tcgggaactt ggtatacata 22920agagagaggc tttaagccac ggaagagaat gagggagaaa cttgatcatt ttgttgcatc 22980ttcatcatgg tgtgacttct ttccgaaagc tacagttgag cacttgatgc gctacaaatc 23040ggaccacact cctattttgg ttcgccttgt aggccatcag tgaagacata agaagaaaaa 23100gacgtagttt tgttttgaga ctgcttgggt gcttgaggaa ggttgtgagg cccaatgggt 23160gagtcatggg ccgggtttac tcgcgaggta tttatcgagc gctttaaagc cgtggaaggt 23220gggttcaaag caaggagtga tgggtctctt agtaatctgg gcccgcgtgt gagggagatt 23280gaggaggcca ttatagatgg gaggcagcga agcagataag gactatgagg ctctatgaga 23340ctcctctccc acgaaagtta gacgaggtgt tggacaagca ggagacgttt tggtttttga 23400ggtctcgtgt gagttagata aaggatggtg atcgtaatac acaatatttc caccacaaag 23460cttcccaaca caaacgtcgc aactacatag cggggatgta tgataataaa ggggtgtggc 23520aagataacga agaggatatt gaagggaata tttcagagta ttaccaaacc tcgttcggtt 23580cgtgctcccc ctctaggaag aacgtcgcgg ttgtccttga ggttgtgagc ccggtgataa 23640ctgatgatat gaatatggcg gttatgaaat cttacactaa agatgaggtg tgggaagcac 23700taaaccacat gaagcctaac ggaatgcatg ccatccttta tagaggttct ggaatacctt 23760ggagatgata ttacatctgt cattttaggt attattcatg gcacccgacc ccagatgttt 23820ttaacaagac taatattgtg ctcattccta aagtcaaatc cccaaatctt gtttctgagt 23880ttcgcccgat tagcctctgt gatgttatct ataaacttgc ctcaaaagta cttgctaaca 23940gattaaaaca ggtttgcctg acattgttta tgataaccag agtgcatttg tgtccggaag 24000ctatattacg aacaatgctt tgatttctct tgaattattt gactctatga aaaaatgata 24060cagagctagg aaaggttttg tgtcgatgaa attggatatg agtaaagcct ataaaagagt 24120tgagtggtgt tttttcagta gtgtgttgga gaagttggat tttgctgaat catgagtgaa 24180tgttgttatg agatgtgtgt cttttgtgca gtactctttt gtggttaatg ataatatatg 24240tggagctctg acaccctcaa gggggctttg acagggagac cctatatccc cgtatttgtt 24300tatacttgtt gcagataccg ttttagctct tcttagcaag gcattcaaca atgcgtggct 24360atacttgata ttctcaacaa atatgaggca gcatcaggct agaaaataaa tattgacaag 24420tcaggaatct ctttcaataa aagatttgac gtattttatg gccatgaaac aagttgagaa 24480gcatcagaaa gacttggtat cccaactttg gctaggagtt cgaaaaaagt catatttgct 24540gacattcaag agcgaatttg gaagaagctg cacggatgga gagaaaaact tctcgcgggc 24600ttgaaaagaa actctcttaa aagttgtggt tcaagcaatt ctaacctatt tggtgggcgt 24660ttacagattc ctaaccagta ttatccaggc cattcatttg gccatggtaa agttttggtg 24720ggggtcgaaa agggcccaca attcgatgca tctgggggga tatgtgctca ccaaaatgtt 24780taaggagcct tagctttaaa gacttagggg tgttcaatga acctaaacta aggaggaatg 24840cgtggcattt gattcctgct ggtgagtccc tttcgggtcg agtgttctcg gccaagtact 24900attcgaagtc aacctttttg gactcatttc taggtccggt aggtagcttc tcttggaaga 24960gtatttgggg ggccaaggca ttagttaagg gtgttttatg gtgcgtaggc aatggcagac 25020aaatcaacat atggcgtgac tcgcgggtgt tgaatggtga tagtaggttc atccccggag 25080agcgcgtttc aggccttgag gatgtttgtg atctaataga ttttgcacaa tggagtgcga 25140tgtggacctt gtcacgattg cttcaatgaa gatgatgctc aagccatttt agtcatacct 25200ctaagtaagc gccttctgaa ggacatggtc tcttgggctt tcactaagga tgaatttttt 25260ttgtaaaaac aacctatatg gccggttggt cgaggaattt gaatttgttt cacaaagcat 25320ggctgcaaat ctggggcctt aacgtgtctc cgaaggtctg ccacttcctt tggcgtttat 25380gctcggtacc cttcctgttc gagctctttt aaaacgacgc cacataactg atgatgattc 25440atgtcctttg tctaaaggag cccggaaagc atatcacacg cgttgttcta ttgcccatat 25500gtagccgaag catgggagag tgcgggcctc acaaattgtt tgcctttgtt tgatggggct 25560ggtatgcttg atgcgtgggg ggagtgggaa acaatcgatg actagtccct tgtaagactt 25620agcttcttgg cttatcactt gtggtttagg cgaaataaat gtgtttttga aggggtggtg 25680agagcgaatg agagtgttgt ggaatatgcc actaaagcta ctgttgatca tggtttgtat 25740agtgcccgca tttatggtgg gtcgaaggct accgcatcca aaagctcgaa ggtatgggtt 25800ccccctccag cttgtcgtac gatctaaagg ttgatgcatc agtggggaat ggtggatggg 25860tggggctagg agtaatcgcc tgaaactaga aaggggaggt gctcgtggct gcaactagga 25920gggtcagagc ttgtggcccg tggaaatggc tgaagggaag gctctttgtc ttgctcttag 25980gcttgcctcg ctcatacaac ttgcaagaag tgatcgtgga gtttgactgt caatcttggt 26040gaaccatctc tccaagggtg ctatttactt tgcattttta agtcaaagct tgaaccttga 26100taaaaaaatc ccgttcgaca tgaaaagtgc cttgattttg cgggtttggg agtgccttat 26160tgattctggg gtttgatttg taacaccttt agtaaaaaca tgtaagctaa ctgtaaaacg 26220aacattaatc aaactaggat atgtaaaatt cctaaatcaa gaagaatttc cacttgtgct 26280gaatttgtcc accttgcatg acacccaata aaagcccatg tctcctagaa ccccttatgc 26340cgccttattc atcttttctc aagttgagtt ggagtcctct atggtccact cgacttcttt 26400agcacactct cggtaaaaac ttttaatatt attttatttt agactccacc atcttgacat 26460ttattccttc ttaaacttgc ttcacacaaa catctaacac tagaattcta tatagaatag 26520cttgaatctc tcttaggata accttatagt aaatgcaact acgcctatcc ttaaaccttt 26580ctaagaggag ctttatcgta tttacattcg cttcactttg aaacgtcgct aagtgtatgt 26640tgcactttcc aaaccatgtg ttagctaaga ccaagttata tgactgcata acctaaatag 26700tcttcctcga gaaaattcac tagttggatc ggaagagttt gtgtaaatct atggcggcgc 26760gggactgggc cttcacgatt tcaagtgttt taatgcagct cttctaggta aacaagtttc 26820ctaatagcgt ggtgactcaa atattgagga cttgttgtta tactaatgct attcctggcg 26880gcacttaagg ggtgaaatag gtggtacaaa ggagtgcttg atggcgtgtg ggtagtagtt 26940gaatatatca gtatgatcaa gtccatggat ccctcgtact tattcgtgca agattatttt 27000tccacgaggc aaagcgagcg agaatcttaa ggtttatgat catattcatc ttgtacgtgc 27060taagggtaat gtccctttca ataatgagct atttctcctt ttgagcaaga gcgtatctta 27120agcattcctc gtagttctcg tctccccaac gatgttttat gttggaatct gaatttggag 27180aaagacggag acttttcgtt cggtctatcg agccattctt ttgagttgga tggcgagagc 27240gtgatttcat cgtcaatacg ctctaattta tggagtataa tatggcagga tagtaccttt 27300caacgtgtta agcttttatc tgcattgtcg acacaaaggg gattgagtaa gcctgtgccg 27360agtatggaac cattgtgtaa tctttatgcg ttggaggatc aatggagcta cacttcttac 27420gagactttgt tattggaagg gcttatatgg gatccaacta gggtagtcaa aacattggtc 27480ggggctgcgc tgcaattttg gggacttggc accggcgttt ttggagtagc tccctcatgt 27540ggaacatagg cttttgatga cgatatacta ggctagatgg aatataagag agaggtgttt 27600gtttgaggag gaggtttgtg atccctatca aaccacatgc ataatctcat ggcgtagctt 27660catgtggaac actgacatat tgcatgtgta gggttaggtg cttgcctagg acaccacatg 27720ggaagagctc cgttaagctt aatgtggatg gagggtgtgt ggaagggttg ggtgcgtcca 27780ctggagtggt gattaggggg atggatgaaa aagcgttgta gttgcaacat agaaaggtgg 27840aggactgcga ggaaccgtta aaaaggctat attttatggt gttcatttgg ttgtggaagt 27900cgatttttga aatatggttg ttggaagtga ctatcttcac ctcgttgaag caacttcttc 27960aaaagtggaa ggcaaaaata gcttccatgt tattgttgat gacattgttc atggtagtgg 28020tatgttaaat acttcgtctt gtagttttgt tcgtagggat gggaataggg tttctcacga 28080actcccccat ggaaatatca taatggctca tgctcatagg taaatgaata agctaacaaa 28140attgttcatc tttgcagaac taactcatgc atgaatcgat ttcttagctt tagtgaaagg 28200tagacagctc tagagagagc atctagtctc aaaaatacca tgagattctg tagtgtcctg 28260acatgtttta tatgacagga caaagcgtta aaggagcaca acaacttgct atccaagaag 28320gtataagttc agcaagattg tttagtaaca ttgttaatct tgctgattgc tttgaaacat 28380gtcttgctat ggttaacaat gttgactgaa ccaaaatagg tgaaggagag ggagaaggtg 28440ctggcttagt aggcagaatt ggattagtaa aatcatgaca ataactcatc tggctttgtg 28500atgtcttaag ctttgccctc actgaataca gggtcagtcc tcaataacct ctaatcattt 28560ttccaagatc caaagtaaac atggtttcat aatttaatta agattttttt gaaccatgtc 28620tccatacaac cttactagga ctaatactac taatttaaga ccccaacgat aaacaacaat 28680aattagccat atctggctag caccttttgg acaacacacc acatgagact cttggccaac 28740ttctttgatt tccttcagtc tgatagatat gaatatcttc tgaagagctc tttggttcat 28800aattattgat ttagaaaaga attcagcaag gtgagtcatt tggtaacctt aaggtcatta 28860tgggggtact aaatcaaagt gaagatatat ttaggtggca tcagaagaga tgatatagat 28920aggttgtatc ctgtcgatag gttatttgga tatgtatcaa aagtttcttt tataatatat 28980ctatactgat tggttgatgt atcaaatatc cctacagatt gtgaaaaaat cccctacaga 29040ttgtgaaaat atccctagaa cctgtgatga tataagatgt gctccgcatg ctttattgaa 29100cataatgtat tcaattcttg aaatgcagag gaacaagcag cagtgcagtg gaagatgaag 29160caacataacc accaaatcta aacagcaact ctgcataaat accgtcctgg atgctttaac 29220acatctaaga gcagtaa 292373921668DNAArtificial SequenceBvAP1 genomic DNA showing mutagenesis site at position 6999mutation(6999)..(6999)c to tmisc_feature(9833)..(11385)n is a, c, g, or tmisc_feature(12467)..(12605)n is a, c, g, or tmisc_feature(13741)..(14088)n is a, c, g, or tmisc_feature(16396)..(17194)n is a, c, g, or t 39atggggagag gaagagtgca gctgaagagg atagagaata agatcaacag acaagtaact 60ttttcaaaga gaagaagtgg acttgtgaag aaagctcatg aaatttctgt tctttgtgat 120gctgaggttg ctctgatcat tttttctcac cgaggaaaac tctttgagta ttcttctgat 180tcttcgtaag tatatatata tatatattaa tagtaactac ttgttttctg ctttctattt 240ttaggtctga tgcatattta atttaggtaa tattaattcc ttatatctga tccttaattt 300ttttttcttt taccatttca tttttgtttg ttttgaataa aagaaaattt ccccttcacg 360tgtgtcgaat aggtcaaaat ttttacttga aggatgttct ctttgattac taaaatagga 420tccaacaatc acctgaaata aaggaagaag atggtgcaaa gtttttactg tcatacttag 480tatttgataa atattctatg atgaacttgt ataaattagg aaatagacct aactttcatg 540cacgaaaaca ttattccttc attcaatttt tttattactt aaggatttac ttttttattg 600atcatatgaa gtagtagtac ttgtaatcat tcaatttttt tgttggttaa ataggactac 660attttaaaac aacccaattt taaaattttt tgtgtgaatt tcttcccttt ttaaaaataa 720agtctattat catagcttag agtagctgtg gcaaagctag acgaaataat acagaaatct 780ggaaaggaaa ttgtactact tacatgaaca cacttattta ttacttgcat gatatctgcg 840aaaaagttta tagcaaattt ggttaatata tagcgtagta ctttggatat taatattact 900agtgtacaaa tacttgatcc aatgggtaat gaaacttatg gaagatttga ccatacatga 960tgatgctaaa tattaattgt tattgtccag ctttgttttc cctccatcca ttggcatctt 1020catctttaca ttgctactcc actcacttgt caattgtttc gtcctttatg ttctttattc 1080acatgtgcac catacttcaa tactttcccc ttctttatcc tcagtttttt tttcttgtca 1140ttttagggtt aatatccaat gaaatctagt ttgctcgttt tagatctaat tttaattcga 1200tcacaaccat ccatattttt gtttcttagc ttgacatcta ttctatggat ctgggatctt 1260cggtgtatag atgttctcgg ttttcagatc aagatcctat tcatagaccc atttattgta 1320aacacttaaa tgtgttctta aaaagttagt ggctcgccaa gtcaactcaa taacataacc 1380cccacgactt cattacatta cacaatgaaa gattagatgt atgagtttgt gaagcttata 1440attctatttc aagtaggact aggatgtttt gtgcaatcag cagctagtag tctttttaat 1500ttaagtcagt cttcattgtg catcatatat ttttagaaat atatgcaagt ttgaaaccat 1560ttagaacctc atgacccgcc tgactcacta taaaccggca agagcttaat ttttcacagc 1620tttgtatctt tatgagtagc gctagctagg ggtatgggca tagaaaaaaa gggtttgggt 1680tagggtctta caagatctta tccgctattt ttatttcata atctttcaaa atacatgttt 1740aataattcaa aatacatgtt taatactatc tccatttcac aacatatgca ccaattgcct 1800agctatggtc caacctagtt ggtttgtagc ttgcattgga tggttaggat gtattggagt 1860tgtttatgtg caatcaaatt ttaattacgt atcaaaaaaa aaaaaaaaaa aacatatgca 1920ccaatttcca tttggacaca cttattgacc aatttttgac aatatttttc tcaccatttt 1980gtaagaaaaa tcaaaatcaa gtggaatttt gttaagttta tctcagtcaa aagattccat 2040acatcgacat tttataattt ttaatcatac gcaattagaa atatcaatgt ctaaagaagc 2100gtgttggaat acgtgaaaaa gcaaatgata catgaaacag atgtagtata tagaaaactt 2160aattttgtgt cactcggatg tatgtgggcg gagccttcct agaaggcgta cccaccttag 2220tggctctgaa tctttgacga cccgttcggt tggtggtgat aatagatggt aatagtaatg 2280taatttagtc taaatttata aataaatatt aatatcatta cccatggtaa tacaagttct 2340tcacaaaaca tgtttcattt aaaaattatc attactacct tttcaagtgg tattggatga 2400taataaaatt ttaggcaggg aaatgggtat tgggatgaac attaccatgg gtaatgacat 2460gcaatttttg ttacaagaat acagtataat acattactat tgccaccatg tataaccatt 2520aatcaaatgg accgtgagga tatgatgttg aagaagaagt cttaacctct acgctattat 2580ttactagggt ctgtaaattt tcctttttta attataattc ttgtgaaatc ttcttcactg 2640atggtactag cttattagga tgggtttctt tagtatattg aaggctcttg ttgacagagt 2700ataaaaatat ttttggggtc gcaaccatca atttaaactt ttgtttgatt ataaaattat 2760tttttgaaca tcaacaatct acttaaattt ttggttgagt tagttctttg acatggtatc 2820acaaccatca tgacataaag gtctcatatt caaatctcat tcacctctca tttccaagta 2880gaatatttac ctcaggtatg ggtatgaggg aggcttgtgt tgcatgagtc aataacggat 2940cttgaccaat aatttaacag gggcgagttg ataaattaag ttttaatgta aaattttaaa 3000tgatggataa aaacactaat acacaccaaa atataaatat acttttatta atggttacaa 3060agagcttgta gctaatgtaa taaatcaaaa tcccaaaggt gcaattttta agaaattatt 3120tccatttatt tatttgacca ttatgaaatc ttcaagaaat tgagtaagtt tttaagaaat 3180ttaaggtata gttcattaac taaataaact actccagtaa aaaaaaatta ccaaactgct 3240ttcttaagta aaaaaataaa taaatttata ttttatgatt gttaggaatg agtgtgagga 3300aataaaaagt actattatag ttaaataaaa atgaaagttc ttcagagaag aagaatagaa 3360gatagtacaa tcaatgttaa atatttttct aaattagaca aattgatata aaccaaaaat 3420aaaggggaag aagaaagaaa taagtaaaaa aagaaagaag gaaaagaaaa aaagaaaaaa 3480gagaagcaag tgaagaaaaa caaagaagtc caaatgtgtg ttgatgcaag gttcgagctt 3540gcaacattaa gggctcaaac tttcttttac actttggttc actgccaccg tgcccacagc 3600ttgttatgtg acatgaagtg tagtttgctt aatttatctt atacagttat gggggaccaa 3660gcctccaccc gccccttcta taatctgtca gtggttgcat ccacacttta agtccaatag 3720actcttgtct gagaggaggt gatagagtat ataaatattt ttggggcctc aaccattagc 3780tcaatctttt gattaagttg gttctgtgac acttgtacta tatactagtt atatatatac 3840tgtaaaacta gtaccacgag aacagtcctt aatacaaaca acatgccctt aatagaattt 3900tcttagtata cacttaatat aggttgacta gctttttgcc cttcagtatg cacacacctt 3960ttataatctg tatcgttgtc tggtagatga taataaacct cagtattggc aatatatgaa 4020atgacataat ggccatgttt ggtgattaga gtttagagtt tagaggttac agttcagagt 4080ttgtggttag atgattactt ttttgttcag aggatttgac tgctgattta aataattgtt 4140gtgtaaaggt gtttggtaac acttagctta ttgtttagag ttttgtactt tttagagcat 4200gtaaaatgac atttatggac atatgtattt ttttaaaaca aattttagta gtaattatat 4260ggacaaaata gtcatttgtt ttttctctct ccaaaactct catgaaaaag ctcctctacc 4320cagctttttc aaaagagagt tttgatcaga gttttcggta caaaactctc tttagtcctc 4380tctctcacca aacacccaaa ttagagtttt tattggtcaa aactctaaac tctctccaaa 4440cctctaaact ctctctaaaa ctctctcccc caaacacccc caatttctta gaaaaatttg 4500ttgctccttt ttattgcact atatttctat ctccaaacat aaagtttctt ttacaaattt 4560tcatttctac tccataccac ctttatatgg caatataatt tctatgaatt aaaatgttca 4620caagttttga ggtggatttc aagagcatgg acaatatgat catgagactc tccatacaaa 4680aattaccctt aaattttata atcatacacc aagcggtcgt taaagtattg gaagtgcttg 4740agtagtttgt gaaaattaac atataataaa gtgcagatct cccctctagt aagtagtaag 4800aagtagtaag acgatgtccc tcatttgaga aagagaaaaa cccttatcag tttctcttgt 4860ttctttgact gaacgcaagt caaatagaag tatgtaacta ggaaatcctt ggagaaatag 4920attttcttta aaactataaa agtataccta tatatatggt aacccacaaa aatgtatata 4980atctgatcaa tatctaaaca aagtattctt atgttttctt tcatcttgct tatttcctcc 5040ctttcctttt cttactttaa tttgtttact ctctttaact tatttctttg cgtatctcat 5100ttcactttac aaggatatat agttgattat gacagcttaa taaatatatt ttggaactag 5160gatttattgg ttgtcgttgt tattttaatt tctacactga tcggctagag tttctagaac 5220atagggcttt attgaaacca ttagttaaca aaattgaatg acaatgattc aatatgatag 5280aatatgtatg tattagttaa tgtttgatta ttgtttgtat gtatataatc aaagattatt 5340tagtaatact tctatataca tattctatta gaatcactta gaaagaccca ttgaacaata 5400ataaggatag gcagacaagc

aaacaaaaga aaaataaacc tgttactcct tccatttctt 5460aatgttctac tcggaattat agatacacac tttgacacaa attagaaaga gagtgtaaaa 5520agtggatcca tattaatatt tttatttttt taaatgagga gagaagtgtg ggtttattat 5580gtttcaaggg agatagagag cattgaatag tgagagaata tgtgccaaag ataattaaat 5640cattgtaaat cttttgccaa ataaagaata aagcatgtga gtaaaacttt aaaaaatggg 5700cgaaaaagga aagttgagta gaactttaag agacggaagg aatatagaag aggacgtgac 5760agatgggagg aagatcagac atcttagaag gggaatagtt aaatttgaga tagtctttta 5820attaaggttc tcactaaaga agatataaca gtaggggaaa gctaaaggtt attcaaaact 5880ttccttccca tcttcatcac ttcatgtctt tactttagag ctcttaacac ttagcctatg 5940aaattctgaa ctctttgtaa gattagtgat agataaaaga atcttatcaa tttaatttat 6000aaatacaaca ggattcaata aaaagatata gagatctata aataaagagc catactgttg 6060tgaactttta tatctatcaa aacctttgca cattagacgt ggtataacta aatcaggctt 6120atcgaaattt tttaaaattg ttttcattat agccccttta tatttagaag ttctaagatg 6180attgcataga tagttgatgc accgttctgg tcgacttttt taaacacttc tttttgataa 6240attttttttt ttgtattcga atcattattt taggtgtata aagagctgca aatgatctag 6300atgagattga tctcggtttc atttatatgc taatagtgtg ttagatacac actattaaaa 6360aagtcatatg acttagagat tattatggaa aagggatagt gcaccgatat taatataatg 6420gaaaatgaca cacgagttgt ccataataac atgtgaaaag tgaactattt aaaaggtttt 6480tctgacctag tacatacaag gtgcgtaggt ttagctattt tagtttttta gttttatttt 6540ttaaagtgaa gttagttatt gatctgaaat catataacat gtacgtaccg tagatataaa 6600aaactaccaa gtatatatca atttgaaata aacattattt taatatggca aaatcacaat 6660tgttgactag acctaacact gaagaaaact atgtcatgtt tatcaattat gttgcataca 6720gttaaaaaca aatatgttag agaaatcgtt atttgaaata gaaaagttgc gcaaaatagt 6780gattaacatc aaaatatgtt cagaaagttt ttataaatat gtgatcttgc attgtctgtt 6840gactgtcgag gtttatgata atttcccctt tttccaatgc aaaacttgtt gtgctatttt 6900ctaatgatat attttttcaa agtatggaga agatcctaga aaggtatgag aggtattctt 6960acgcagaaag acggctagct tcaaatgatc cagactcata ggtagtgcat ttatgtaaat 7020atagatatac tcttcatgcc caagaagcct gaatttttta tcccactacg tactgcaaag 7080ccaagtttaa ttgaataatt gtcctgttta aattatttag ttttcagtac aataatgtaa 7140tcattagttt gcatgtttaa aaaagaaaag cacaagttct gatcaagtga aatataaatt 7200gtaacgaaag agccaagcta gacaattacc tagctaggag ttatttgtta tcgtttttgt 7260ttttaatttc tagttttttt ttttaaacta gaaaatatag tttcaatctt ttgttatcag 7320ttttcaaaat gacatattta acataaatat gattgatttt aaattcattt attatatcat 7380atttcatttc aaaataagtg aaacacttgt ctcaaaaagc tcactctcac ataaatgata 7440aaagtgtttc acttatttta aaacggaaga attatgactt ttacttttca taaaacgaaa 7500aactgaaata tgacaataat ctcaaatagc ttggagaaac cagatttcta tatatttccg 7560tgatgaaatc acttttcatt atacgtaggt aaactggacc tttgacttcg caaaactgaa 7620ggcgaagctt gaacttctac aaaggaatca taggtatgat ggcaatatgt cataattttt 7680ctattattat ttttgcttcc aaaaccagac catatgtttg tatatttata tagtgatata 7740ctccatccgt ttcattttaa tctatacatt tacacttatc aggtatgtca atgcaaaatt 7800ttgaggatat atatctttag ttttgtattt ataaaaatta taaaaagtac atattaataa 7860aatacatatt atgatgaatc taacaagatc ccacatgacg atatttccgt ccgcgtatga 7920ataacaaata atggccaaag tgaaatttgt gaatagtgta aaatatcaaa gtgtaacaat 7980taaaataaaa cggagggagt agtacttgtt tgtcacatac ttacttattt ttgttctctc 8040cacaatgaaa ctgttctttc taataattaa aaaaagtgca tatgttgatg atttctctgt 8100cactttaagt ggatattgaa tagtgataat ggattacttt gtgtataatt gcatttcaca 8160tttgggtcta attttatacc cttttcgcat atcatgcttt gtgaatagta catatgatgt 8220tcaagaatgt gagaagacat atcatacttt tgatatacct caaacatggg tgtatactgt 8280atagtgaacg aaagtgttag tgtaatttta tttaggaggt ttagtggttt gtcctatata 8340taatgctagt agttatacac catagttgtt gatgagcatc aactggcttt cctaacattt 8400ttttctccat aactttaccc ttaccttaca ttagattact ctaggattac attctaccta 8460aaatattatt actcccatca ttttaggtaa atatttttac tttgattttt cgattatttt 8520caagagttta aaataatgat taaaatttac catgatcagg cactacttag gacaagagct 8580tgactcactt aacatgaagg aacttcagag tttagagcaa caacttgata ctgctctcaa 8640aaatgttcga tctaggaagg taagaaattt tacttgtcta ccgtagtttc ataataaatt 8700agtatttggg ctcgggcttt gccccagatt ggtattgtct tttcaaattt gatatgcatt 8760tttttccatt tccactaaaa tatattaaga aaattcaaca tttaaaggat acaaatataa 8820taatgtggat acttaaagta tgattaaaat ttggttgaga tggtaattgt gtcatgtata 8880atagcaagaa gtcacaagtt caaagctcgt tgcaagctaa atttattttt gttgattgac 8940atgacttatc aacacactgg acaattctaa tcatctagtg gagtagcata tactagcaat 9000ttatgcacgt gatgtgtgcc ttactttttt agaatataat ttataacttt tttgagcata 9060aacaaaggta aaatttgaac attagacata tttttttggg ctagctaaat ttgttgttta 9120aacctatatc acttaaccaa actcctcttt tattatttat tgatttatat tttatttaaa 9180atttttaaaa ttaaaatgat gagcaataaa agaatgttaa gtagatttat taagtatttc 9240ttatattttt atcaacaaag tattttgtgt taattaaatt atttcacttt gttaattgat 9300tgtattttcc tttttaattt attacttgat tgtgtattga ttgatcaaac ataatttttt 9360tgttaatttt tttatgctat atttgaattt atttttcttt catctgtttt tggtagagta 9420gttgatttac taaagggtaa ttaaataaat ttattggggg acaccatagc tcccccctcc 9480cttatataat agagatttgt atagatttat tgtcttcctc aattattgat taactagtct 9540tctatgcacg cgatgcgtgt gttgattgtt tgggtctatt cttaatataa atttcatcaa 9600aatataatta tagtagtgtg atttacaatt attgctatac aaactactgt aatttataaa 9660gttgttagaa attgagataa aaatttagat gtgaaatttt gtggtcaaat tatatttgta 9720attttttaaa ctgagtaacc gtttttctca tcatgtcaag ttactttgtt aatgcttatt 9780taatttatta ttggaatttt tgacccatct ttaaattaga aaaggatata atnnnnnnnn 9840nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 9900nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 9960nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 10020nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 10080nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 10140nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 10200nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 10260nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 10320nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 10380nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 10440nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 10500nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 10560nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 10620nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 10680nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 10740nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 10800nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 10860nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 10920nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 10980nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 11040nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 11100nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 11160nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 11220nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 11280nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 11340nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnntatat atatatttag 11400ctaagaaaaa aaagacattt cattggggga taccataact cccccttagc ttatataata 11460gagatactat acttcttttc tgatagtgtc aaatttaatt ataaatcttt aacattggcc 11520aattaataat tggacaagaa aaaaatgaga caataataaa taaggcgatc ttcacagacg 11580tattaacatg atggtaatta aaaatgttaa tcatagatct ttgtgttatc ttaataatat 11640aaatttacta attagaatgt atcacataaa gtaagtatta atagcagcat aggataattc 11700ttataatgga gattttatat ttttttatat aattatatga tttattgttg aaaatattag 11760ttgattttaa ctggttgttt attcaatgac agaaccaact gatgcacgag tccatttctg 11820aactccagaa gaaggtaata actccatttt ttactctcaa aggtttattg tttttaactt 11880atttcttcta accttttata tatgagaagg tattgggtta gacgcgtctg accataatat 11940taggtcggat gactttcagt tggtttcaat tttatttcag ttggtttcaa tttttgtcca 12000gttggtttca atttttgttc agttggtttc aatttttttt agctggtttc aatttttgtt 12060cagttggttt caatattttt tagttgatct tttttatttc agttggatgt cttttaagtt 12120cagttactta tcttattgtt tcatttacgt gttttattgt aactgaaaac aaaacttaag 12180taaatgaaat aaaataagtt ctaaataaaa gcaacttagg gcctgttctc cccagcttat 12240tttcagttca gttcaattca gttcagttca attcaattca tttcagttca gttcagatca 12300gatcagttca gttcagatca gatcagttct tgacaatact tttactctca catatcacta 12360ttcatttcag ttcagttcaa ttcaattcag ttcagttcaa ttcagtttag ttcagttcag 12420ttcagttcaa ttcagttgtt ttatgccgaa gagaacaggc ccttagnnnn nnnnnnnnnn 12480nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 12540nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 12600nnnnnattca tttcagttca gttcaattca attcagttca gttcaattca gtttagttca 12660gttcagttca gttcaattca gttgttttat gccgaagaga acaggccctt agttttcagt 12720tacttatatt atcgtttcag ttagttttct tattctttca tttaacaact aactaaaata 12780aaaaaaaaaa aactaactga aagcaaaact taattaaatg caaaaaatta agttctaaat 12840gaaaccacat acgatcgaaa tttcaatcat ttcaaacatt atggtgtttt cgattctttc 12900aaagaaggca agctgctccc gctattctac cctctttaga tcacaataaa gctcaggcct 12960cacattcaaa gtttcctcaa agatggacgt tccaagtatc acatagacac atagtcctct 13020tctccaaacg ctctccttcc tatcttgatg tcattagcaa acttcttgat ccagacggcg 13080ccaacaaccg caccatgatc tccctctaaa gtactgacgg cccgtttggt tgttggtcat 13140aaatgatggt aatgggaatg aagttgtgtg taaatttgtg aaaaatatca ttgtccattc 13200ccatggtaat gctaatttat cttaatgtgt ccactttcct tctagaattt tcattctcat 13260ccaataccac cttgtaaggt ggtaatgagt ggtaatgaaa attgcttccc cttggagaca 13320aaaatacaag tttaggagtg agattgattg ctcatggaga aaaaaagtct ccccatggag 13380atattaaggg tgattcccta ataaaattac acttaaaatt tattcccatt accgcaattt 13440attaacatct accaaacggg ccgtgaaagt cttgaaacac atagtcgagt gagtagcttt 13500gaggaaccat ctgtaaaaga acctgaggga gccaatgtgt gcgtaagtac caacggcgtg 13560ttgtcagtgg aaaaggtggt gccgtggtgg cactcagtag tgatggagcc gccgtggtgt 13620ttgagtgttg ccaaatacaa aggcggaatt tcgtaatctc taatttcttc tgtgaaattt 13680ttgggatcag cctgtccgac caaacacatt ggatcaaacg gtctgaccca atagtttcaa 13740nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 13800nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 13860nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 13920nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 13980nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 14040nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnga tataaataga 14100gatggaggac aagggtcttg gttttgtcat gttgtcaaag agttgaacaa tggttttttc 14160gtattgttaa aaaattaaaa aaccagaggc cttcaatctc ttgatagata tagatagaaa 14220aggaggacaa ggccgttctt ggttttgtta tattgtcaaa gagttgaaca atgatttttt 14280cgtgttgtca aaaaattaaa caatgaaaaa agatggcggg tgcttgatct aatagatcgg 14340accatggatt gaaggtcttt taacttattt tatatatata ttgaacttat ctgaagatta 14400tttaactctt tgaaattgta ttaacttccg aactttatga acttttttaa ttcttcaaaa 14460cttatctaca ttttatttga aaaaatattg aagacaaaaa aaccctcagt tggtttaaag 14520ctgcggtaag atagagtgta aatgttattt ttttttatta aatcaagaaa taaaaagaaa 14580tattaaataa aaagaattaa aaatggaaat gatgacagaa acttatggct tggaggagca 14640atacttttaa gatagaccta aaccttaaat aagttaaaat ggaagtaatt tttcagtaga 14700atcttattcc aatctatact ccgtgtttac tccatgtaat gcacatataa taaaaaaatt 14760agaaattaca tagtataagg tttgatcctg tgactgtaag tttatatact aacttcttaa 14820ccactagagc aagtgatatt tagtgttatc attttaaagt ataattttaa caaatgaaat 14880ttttttctta cccggaacat agctcggacc taataactag ttgaacaatt ataatctgta 14940acttaaaatg atcctaatta ctgtactttc attacctata ataatagaat cttactatca 15000ttggttcaga aaaaaaaaat cttattaaat gttaaccatt tatttgtaat tgaaacatac 15060atgcacataa atgtaacttt tagtttatct taacttaaaa actgagaaaa tgttagttgg 15120aaacttttgt atatatgttt ggataaacga cgctcaaaag taggggctaa aattttagta 15180gataatataa gattatactc catctgttct agatagactt ctcattttta attttggcag 15240tattcataaa taaaggaaat ctttcaaaaa aatttccaat atataagaaa aaaaataatc 15300atgtgcggtt ttgtttgatt cgtctcattg tgtacattag gaaaattaaa cttatataat 15360ttttactact atgtaattaa agatattaac gatacaaaat gtgtattgac aaacttatat 15420tggagtaata ggaagtctat taagggaccg aagaaatatt acgtaaataa atctaataca 15480aactaatata aattctactc cagacaataa agattctgtc ttatattgcc aagatatagt 15540agctatttat tttatcttaa caaacataaa tgtttctaat gcttaaacat ggacatgtat 15600tattttgtaa aatattatgt attatccaaa gttacatatt taaaggaagt tctattgctt 15660gctctctttt agcactgccc aaaaaggtta aagtaatttt ttttctctgt ttaaaaaaaa 15720aatgcattat atacagataa tttttgctag tcaataaagc tatccttatg acttatgagt 15780gctacttgac tagggatgtg ttgtactcaa ttggaggtat acatacacca agattataga 15840gcttttattt tgcctataaa aaatggaagc cggataggat accaaaaaag ctttgactta 15900aatttgtaat gcataaaaat gatgatacct aacttattag ccatacttat ctaagcgtac 15960gtcaatttaa atattgtgtt attgattaat aatgatcctt atatatccat attttgacaa 16020ttaaacggta aattagagag aaaagtttga gaaaataatt atagcttacg taatgctata 16080atccaaagtg tctccgcaca agcgtgggac aaaatagtac tttcggagaa gttacaatca 16140acagctaggg agtcttcatt gttcttgaat agaaggatgg aaacaaagtt caccttcttt 16200tattaaagta ttaaggtttg ttattagctc aatatccaat actttctctg ctttttatta 16260cttcgtctgt ttcaaattaa atgatttttt ttttatttta cactattttt aaatttcact 16320tttaccatca tttatgattt atatgtgaat gaaaacatag ttacgtgtga tcttgttttt 16380tttttttttt tttgtnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 16440nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 16500nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 16560nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 16620nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 16680nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 16740nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 16800nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 16860nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 16920nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 16980nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 17040nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 17100nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 17160nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngtacat caaggaaatt tgcattaatg 17220aaaaacggga gtacaaccta atggtaatac aaccaaacta aacagaaaga agaaacaaca 17280gacagtaaga aaacctctat aacgcgtaaa aacaatttaa cataaacact aactagaaaa 17340agtccaggcc gaataacatt tgtcttgtgc gatggggagg gaagatgaag gagagtgaaa 17400atctgttgaa agacacaact ctgacgatct tcatagagag attgtcgtac aactgctagt 17460agatcctgtc cactaggcac gaaatcccaa ggtccttgat gagcctcctg ggattctcct 17520gattcgttga acaaagaagt cactttcgag agtaacttcg gaccctgctg cgagggtctt 17580cgaaaatcag tcacattaga ctgaaacccg aaaaacaagg tggaggtctt gacgtgcccc 17640aaaacaagtt gggggcgtcc cacaagatgc gttttttagg tgtgatgaca catgatgtca 17700tcacgagaaa ttggggcgag ttagtttgat gaactacgcc ccactgacgg atcctaagat 17760ccaaagtgac aatctcgaaa ccagaagacc gacaaacaac agatctgaaa catacaaaca 17820tgaaaataat gaaagcataa actgccaccc gacatataga gctccggcaa acaacaccat 17880caagaacttg caccaaagac ttccttggca tactaaagac actgattcca actaacacta 17940gcgggggacg gggaagggac actcgactac acctaaacct aaccagggga cggggaaggg 18000gaacttagac taaaccttcg taaaaagggg gggatcgggg aggggaaaac cttgaccaag 18060gaagctggtt ttaaaaacca cttagccgag ccaaaaaccg tgggtgggaa gaagaaacag 18120accacaaaca gggggaaccg ggggatggga actcaccgaa caggggaggg ggagaaatcg 18180cacagactcg gggaacgcct aaggactggg ggacgaccaa cgaacgaaag gttggggtgg 18240tgcgaaaaca agggaggggg acgcaccgac gaacaaaaaa accgacgaag aggccgaaaa 18300agcgaaaggc cgacggagat aagattgaaa ggcgacgaaa aaagaaaaag gaacaaaacg 18360aaagaaaaac gaactcgtcg gagacccgcc ggagacctac gcggcgccgg atctccggcg 18420agttctaggg ttagagggtt tgttgtgttt gtttagggag aaggcagagg tttttttttt 18480ttacgtgtga tcttgttaga tttgtcttaa catgtattct ttaatatact ttttttttta 18540taatttttgc aaatgcaaaa ttagagatat atgtcctcta aattttacat tcacatacgt 18600gataaataag agtgctacaa ctaatttgaa acggataaag tatttgaatt gtttttcatt 18660taaaaaagtt cgctatcatt tataatgtta tatatttgcc aatatgttat ctctttctct 18720ctcttaccag agtttagatc cagtagagtt agtaaataat tctaccacgt agagttgaac 18780aaatcatagc cattgatttt caaatcattg gtttatatat tctttcccaa aactcccccc 18840tattttcccc aaaaatcctc cccctcctta tctctttcca taaaatctga gtcgttgatt 18900ttaaaatata aggtttggat tcaactccac tatgtagagt tttcatcaaa ctccaccgaa 18960tccgagcccc tcctaccata gtacttcttg atttccccat atttctttcc tcatcttggt 19020cctcaagcac attttaatat tatgggtatt aaacaataga gaaagtattt acttatagag 19080aaagtatttt caatgattcc ctaaattttt ttttgaaaga aagaaaaggg atttcattaa 19140tatttcgcca aacggcactt acaagtcatt tctgaaaaac ataaaattct aaaagaaata 19200catatcaccc tagaaatgta aacatcgcag atttgactta attttgcctt aataaaaatc 19260ttcatctgaa gcaatgcaat ctgtgagttc gctctggttc ggcatacgat ctgcagatgc 19320ggaaattttg ggatgaacgt actccaatag tcttccataa ttttacagaa gttgtgaaac 19380cctaattctt catgttgaat ctcgaacttc aaccaatgag aataatttct catacctaaa 19440aacaaaagaa ccatactcac aactcccata ggggagaagg agatttccaa aacagaaact 19500aaaaacccca taaaagggtt tgagaaaatc tcataaagag atactaattt attgaacaaa 19560acaagaaaat gaactaaaaa ctgaaaataa aagggaaaaa ggggcttacc atggatgaaa 19620acatccatgg cagcccccta attgatgaag aaggggtaag ggaggctagg gttttagaga 19680gagaaaagga gaggggaggc taggttttaa aaaaaaatat aatgattccc taaatttact 19740tatatatatt taccaagatg acgtgatgtt ttacaaggcc catgattttt acgcgatcat 19800gaaaaacaca gccaatttga atggagcaaa tatctacgcg tcattttaga tatttttgta 19860tgggaaagtt ttttttgacc aatgtaatta ttaagaagca tcggccaccg ggtagataag 19920atgtcactat acatcctttt ccaaacttaa gtatgcctgt tgaacttttg ttgcgtttgc 19980agattcattt gaaattatat ttcctcagat cctctacttg taaaagaatg ttccattatt 20040ttcttagttt acatgatatt tacaatagta tttgtctaca ttttgttcat attacttagt 20100gatcagtgta tacgtcatat attagtttga actttgaaga catttatttt ctatatactt 20160cctttgtctg ctaaattact ttggaaagct ttgttttttt tattaatata agaccctttg 20220gagtttggaa atcactatct aatgaaatat ataattcatc attagaacaa aaatacaaat 20280atcgtactat cacctatcat gttccttttg gatttcgctt cacaaaaata cattttaaaa 20340aaaaataaaa taacaaatgg tagctaacaa cttattactt ttaaaagttt gtgtgcaccc 20400taataagtac tcaaagtagt atgtaacaga gagagtataa tgctaaaata caaactaaat 20460aaacaagaaa gtgtttctca

acaataattt gctgcaggaa ttaggaaaca aagtaaataa 20520attgcatgtt tatcatcaat acaatttact ggtagttaat tacaaacttc actcatgata 20580attgaaagag gccactcaat ttcagctagg agttgtttat ttatttattt ttctttcagt 20640taaattttga ctacccacaa aatcttcatc tggacctaat ctgcaatttg tggattttgg 20700atgaaatttc taacctattt aagtagtctt attgtttaaa taacccatgc aattaaatta 20760ggttatatgg gggtgattca tttaccaggc ccaagatttt atctcattct caattattat 20820cgcaacaccc atgaacctaa gccaacatga cttatttacc aggccagcta gagaagaaca 20880aggttgctga ttttcttgtc cgtgattgta gaagaaatgt tagaaatcta aatgttgtta 20940gggatttacc cctcccccct actgagtgta tgaacttatt attgacggat tgttgtaggc 21000ttccaagcca aaactctgat taagttttct tttatgccat tttaaccaaa aaaaaaaaaa 21060aagctaggaa gctagctcag cgcgctctaa ttatttcaca tgtgacatgt tttacactta 21120ttcatacttc tatatgcagg agagggcaat gcaggagcac aataacatcc tgtctaagaa 21180ggtacttgca cttgaccagt ttgtgtaata ttgtaattta atttcttaga ttttggttgc 21240atgctttgat gacgaatgac gattgacgaa tacattttta tgcagatcaa ggagagagga 21300aaaaatctag agcaagtgca acagatgcag tggcagaacc agcaccagca ccagcaccag 21360cagcagccgc caccgccgcc acaaaatcat caagttcctc ctgatgcatc aaatttcatg 21420ctcccacctc caattccttc tttgaacacg gggtagttac ttcttcaact taatttcctc 21480tattcaatat taagttaaga aacagatcac gtgattagtt cgttaatatt gctaattaat 21540aatcatattg ttatatatca tgcattagtg ggtaccaagg acaatttggt ggagaagtaa 21600ggaggaatga tcttgacctg acgctagaac cgatatactc atgtcacatg ggatgcttta 21660caacatga 216684029237DNAArtificial SequenceBvFUL genomic DNA showing mutagenesis site at position 19552misc_feature(1029)..(1924)n is a, c, g, or tmutation(19552)..(19552)c to tmisc_feature(20703)..(20722)n is a, c, g, or t 40atggggagag gtagggttca gctcaaaaga attgaaaaca agatcaaccg tcaagtgacc 60ttctccaaac gtcggattgg attgttgaag aaagcgcacg agatctccat tctctgcgat 120gccgatgtag ctctcatcat cttctccact aaaggcaagc tcttcgagta tgcttctgat 180acctggtatg tctaatttta taacttcttc ttttgtacat caataatttt atcatcgact 240caactaaaag cttaagcaga tggttagggt tctattatta ttgaattacc tcaaatttgt 300catcgactca actaaaagta gagtatattt catgtagatc aggtgctttt tttgaatata 360ttgtcagttt tagaactaca aaatgttgaa cacaagtatt tatacgcacg ctgacatgtg 420aattttttaa ttgacaactt tctaaattaa tactctaaat tactaatatg aagaacgtaa 480tttattattt atcactttca gacaaaggca tgtttgtttt ttctattatt tttcccatga 540aaattctcac caatatccga ttctgtatgt taattttagt aatttctaat tttgatgact 600taataaattg taaaaaagta taaaataaac aaatatccaa aacatctttg ttttcaagag 660aaatatctta aaaacttttg ttttttaaga gaaatatctt aaaacacttt ttatcatact 720actatgatga tgtataaatc tattcaaaaa aaaaaaaaaa tgatgatgta taaataattt 780aaagagttaa gtttattaga aattatagat atttatagag ctgagtaata aaataatact 840ctacagatta tatgtagctg atgtagtgtg tctgctcctg taagatttcc tttttatctc 900caaaaaaatt gcattgatat tcgagccttg ccgaccccct tttcctcttc aaccatttga 960taagatccta tgcactgagt aatctagtat tatatgttag atgttatata ttaataagct 1020aaaattgtnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1080nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1140nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1200nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1260nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1320nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1380nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1440nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1500nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1560nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1620nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1680nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1740nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1800nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1860nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 1920nnnngatgat attatgtatc attattatca gtcatttatt attgatagat aatgttatta 1980tttccataga tcatatataa tcttaccata tttactccct atgattatat atttctatct 2040attaacatag taatagtgat atactaacaa cgagtatctt tcaagtaaat aaatcatata 2100tatagggtta gaaggtaaaa aagacaatat tttatgagac tttatttaca cccttcgtct 2160cataaactcc tttctatttt ttgtgttcac tcgttttcta aagttttttc tacttctttt 2220actttttatt ggataaatac tttttccgtc tgtatgtgat ctacttatat tagtatttat 2280agatagatta tactcttaaa gtattacatt cacaaaatca tgtgaattat taattaaagt 2340aaaattatga gtatggtatt ataacttaaa cgaagtagtt gtattgttta aaccaataag 2400tataataact tataaaactt aaaagttgat tctacactta tcatgcactt gtgttttgat 2460gggttaaaaa ttagctagta cataagtaac ataacataaa cttatctctg tatagtatgt 2520tgaattatta ctttatattt gaaaagaaca aagacacaat aagtccaaaa gatccgatct 2580tttgattatc aactatgtaa gtgtctttcc taaatgatca atcaccttaa ttagtatact 2640aaacaggaca attatgacat ataacacttt tctatttgta caagttaatt aacccattga 2700cattcattca gttagcctac atttttcaat aggtagtcat catcattctt tttttaacca 2760atttttttac aataaccatt acctaccaac aacattttca aataatgaac tgaatgatgg 2820attccgtcaa ggaaattgtt cgtggacagt ttgtttacct cccaaatttc ctttaatctc 2880atgctttctc catcatccaa acaatctaat accaatcgtt ttctctaatt gcataaaccc 2940taattgttga atcctttaat cttgcctttt cacattgccc aattccttta gtatgatatt 3000ttttattcga taatccctga taagtaaatt cactaatcta atttcagatg gattgttgta 3060gagattgggg aattgaagat tttttgcctt ctttgttctt gtttacaatg aaggatgaag 3120tttcatggaa tattgaagag aaatttgaga aaagaaagaa agtgtgggct ttactgacga 3180caaaaactgt cattttggtg gtttttttca ctaaggcatg tttggcacta gcgtttaagg 3240tagcggttag cgatttgaca agatcaaaac gctacttaag aaaatgatga gtgtttggta 3300agatagtggt tgttgtagca ggtagcaatt agagtagatt atgagtagcg gttgtggaat 3360gttactacaa gtaacgtttg agatttagag gtagcagtcc agcaaagaaa cattgtataa 3420tagcataagg tagaattaat taaccaatgt ttttttattt ctttttcctt ttattgttta 3480ttaataattt tattttatgc caattcaatg tttattttac aatagcaaaa atgtaattta 3540aatatttatt ttaaacataa taaattttat aagtatttta gtaaaattgg taatataaat 3600ttttcgaggg ttgaatattt tcttagatgt atacatttct ttgaatagtt aaaaaatcat 3660atctcctttt tgtgtaactt ctttgaaaaa taaatcttga atttaactat tgaacgaaca 3720tatgaaatta ttgtagttca tttcatatta taataaatgt taggtattgt cttttacaat 3780ttcaactact tttggcaaac aattctgatt aaacagctat tttaatcgct gatcgttgac 3840agcaaccgct aacagctact agaaccgcta cttttgccaa acatgcctaa accaagtaat 3900atcggggatt ctttattata taaaaagtta ataatgtgat tatttgaaaa aaaggttgac 3960tttatatgtt gtttaaagaa aaaaaaacat ttattaatag aaaatatact atataggtct 4020ctatataccc agggcgaaat gaatgtgtat cttttcaaat gagtatgcgt acatgttctg 4080taaatgcata tttcatatga gcataatgtt tttactatta ttatgcacat ttgtgtttta 4140atttttcaaa tgagtatgta aggaaaacat gtattcttgg catgtcagtg ttagtgattt 4200ttgttgttat ataaatgttt tcgtgatttg tgaatgtggt acaaacattc atgtgccatg 4260gcgtttagca aaacttttct agctcacatg atgcttcaag ctaattgcaa tgaactaata 4320taagggagag gacattttac gaattagttt tacattgata gtagtttgaa gaagatagtt 4380taggagatag tttgttggaa tagaagctat gtgttagaat tagttatagt catcaatttt 4440tgaataagac tcattattat ttcaatcctt ctacctttta attactagtc caactctcac 4500tctttggtat taaattacac cattcctacg gactatctaa caactctaac cacggcccat 4560actttttctt cttaacataa aataatatta cgcttactaa ctactaactc ctatgacatc 4620taccttttca ataaaataac aattgataac tatataacaa cttataatct aaaagtaagt 4680gtcttgataa gtgtagagta ttgtgggacg aagggagtaa ttcatagcaa atactatcat 4740agcaatacaa tactaggaga ctaagatgtg agttttgaac ttcaaaaaaa aatatacgcg 4800acatagttca ccggaagaac tgcagcacaa caaatgcaaa tggggattaa atgaggagtt 4860cacctacatc acacacaaga gcgattgagg attttcagat ctggaagaag agcgaaaaat 4920caggcgagag cttcaacttt cggagtttaa tggaagagct aatgatgatt gaattattca 4980gttggattta tcttttgtaa gatgagggga tgaagttgaa gattgccata ataacccttg 5040agggcgacac aatggtggtg ggaatggaaa tatggaccac gaccgattta tgagtggaaa 5100gaattgaagt ctctgataca atgacgtttt ggtatatcat cggtgctttc atggctgtca 5160gatctaccga aaggaaaagt agtggtgaaa acaaatgtcg ttcaaaccaa ccacctttga 5220ctttcaatat tgaagtgaca aggaaaactg gcaccttagc tagtgacttg gtggagtctt 5280tgcgtaaacc ggtggaaaag aagcttatct ttgagtttta gtgtggtctt ggtgcttgtt 5340taagtggctt gactatttag gaggaaaaaa atcaattgtg aacaattggt gagtaaccta 5400aaaatcaatt gtgaacaatt ggtgagtaac ctaaaaatca attgtgaaca attggtgagt 5460aacctttaaa ccagactcaa gagtgggaaa gaggcggtta gacaagtaga tttagaggaa 5520gaggaggtag aaaatagatt agaatttttt ttgggagttt tcaaatgtag tggtatcggc 5580tcataacaag gtggtcaaat ggatggagag cttggtcgtt tgagtagtct catggtggtg 5640attataacta cgttggcgat aaacaagttc gctttaccaa gaagttgatt ggtggaagag 5700agattttcat aaagaggggc tcgtatagca acagctttgt gtggtctcag tttcattgtc 5760ggcaaagccg tgggtggtaa atatggtgat tttggagatt gttatgatgg agggagttat 5820ggtggtttca tgagacatgg atgctatggt ggtgctgtga tcggtggaga agaggagctc 5880gtgaaaggtc actctgatgg agcgggtgta atggtggtct aggttttttc ggcatcggag 5940gttgcgtatc tcgtgaaggt tcacatttgt acaccggtga atactatggt ggtgaactag 6000gatgacagtc aaggtgacca tagtagaaaa aaaaaacata aaccatgtag cttagatgat 6060ttgaaaaaaa atcatgtttt tggagaagaa tcttaaatat tatgacagag gcaaacttgt 6120cattgaccaa atagatgaca tagcaattgc acgtgtctcg ttaaaatttg aaaccataaa 6180aaattcaaat tgcacttcat atgcctttct tttggttgaa aacttcatat accctaatgc 6240gtcaatatgg ttctttttcc aaaaaaaaaa gtaaattatt tcggcgttag taaaagcagg 6300tccacctcca taatccattt tattaagcca actcctctac cctacttttg caacctatca 6360tttcttattt tctaaaatca tatcaaaaaa acaagtgtga acccaaaact aactatatta 6420tacctaagtc taatttcttc atccaatgtg ttcaacccca tttttcaacc cttccactca 6480taaacccatc ttctttcact cctaaaactt tcagctcacg ctcgtatcac ctctttactc 6540acatatcagc ccaaacgatt tcttattgaa tccactaaat tatgtatatc gattttttca 6600ccaagtactc cgagttttca aaaaatttac ctggtacccc caagttttca aactacacgg 6660gataccccta agtttcaaac taatacactc agataccctt aatgactaac gacattaatc 6720gccgttagtc attaacctta attttctaga tttcaaccta attaaccact aaccctaacc 6780ccaaccctaa ccctaataat aaccctaaac ctaaccctaa ccacccctcc ccaaccctcc 6840caccacccct gccccccatc ctccactcct gcgcagccag caggcccccc acccatttga 6900ttttaaggaa gaaacacgta tcagggaagg gggagaactt agctctgaca gcggcaacgg 6960accaccgacg agtctggact gtaggacggc agcgtcaagg cgcgagccgg ttgggctact 7020gcagttcaaa gcaaacaggg gaggggaatc gagccgagaa gagagctaag gaaagagggg 7080tgatgggcgt tgcggagatg gtgcgcatag tggtggtggt gtgggggagc tgtggtgggg 7140gagaaatcga atgggtgggg gagagagggt ggggggctgc ggtggggttg gggctggagg 7200gggtagggtg ggtgggtgga ggggtggtgg ttgggtggta gtgggacaac tctcaaacat 7260gattctttct caaacatgat tctttctcat atcctttttt ggatttcctt aaaaaaatcc 7320atatccaaat aacgttgacc ggtgagggac aactctttaa cactattctt tctcaaatct 7380tatgaaatct tcacatttaa ctcgatctcc ctttcaatga acctaaagat atatattaat 7440agagttgcaa attcctaaac tctagaaaat tcaaatacaa cataagagtc ctagattctt 7500ccacaagatg tattatatct ttcaaagttt cccagataaa attagtaatt aggaaactcc 7560ttaacaagga tacttaaagt tttatctaaa tcttgcataa attgaaatcc aaccataatt 7620atgaataaat aatcataaag aatcctaaca taaataacta gaaaataaga taataaagaa 7680gcaacaaaag aatctcataa ccaccatttg aatcccgcat gagaacccaa atagttgttg 7740ttccttataa aaacccacca cctttcttcg ggtattatga cggtattgga ctatagtatg 7800agacgagatc tcttaatcac caatcaacta ttgtaaactt gtgagcctga ataatttatt 7860tgagatacaa ttctaaggtt gtttatgaac gtgtttggta aaattgttat tgataactcg 7920ttcgtggaaa ataaatgcaa aagtcaacat gccaaaaaaa gtgctaaaat caactttcgg 7980ctttgcttga aatgttaagt tttaagctat ccaagagcca ttagtcaaaa tctattgaaa 8040gcgtactcaa aaaccattta tcaaacaccc ctacaaatcc ctttagaaaa caataggagt 8100tgtacaatat aagtattgag ttataaagtt gatcaagtga tttaggaggt tgttccaaat 8160caatctacaa gagtttgtat acttataccc cttcgttttt ttaattgtta cacttaggcc 8220ttgtttgaca aatagagttt agcggttaga gtttagattt tgctgttaga gttttaactt 8280tttgttaaat agatttgact gctgatttga caacttcttc ttataaatgt gtttggtaat 8340tattaacaga ttgctaaaag cttattaccc tttatttatg tgaaatgaca tgtatagaca 8400ttttaatcca catgggtatt attattatta ttattcgagg catacaagtc attgaatata 8460tttttaccta atcctctaaa gaaaaagctc ctattaggag ctttttcatt tcgagagttt 8520ttattccaga actctcttca aaactctttt ttaccaaaga ataggagctt tttcatttca 8580agagttttta ctccagaact ctctttaaaa ctctttttta ccaaacaccc cttttagagt 8640ttttgactag tcaaaactct aaaagtggtc caaatttctc ttttaactcc aaaactctaa 8700ttgccaaaca cccccttaca cttttcacgc ataccaatgc aacactttga cgattaacat 8760ctccagtttt ttatttgtaa aaattataaa gagtgcatat taataagtag ggctgttcaa 8820agtgcggtct ggaccgcacc aaaccgcaac ccaaaccgtt gtttcgcggt ttggtttggt 8880ttgcggtttt aaaattgcgg tttgggttat gatttcaagc aaaccgcggt ttgcggtttg 8940ggttgggttt ttatttttgt aaaccaaaac cgcaccgcaa accgcaatgt tacatttttt 9000ttaaaaaaat aaattaaata catttatgaa ggtgacatac aattataaaa ttgaaaaaag 9060aagtttgagg taaaaaactt taacacttat gataaatcat tatatatgtt taattatgaa 9120ttcagcttca tatctatttg gactcttatt aacaattttc ttttaatctt aggaaacaaa 9180agtaatgtcg cggaggaaat aatggttaaa ccgcaaccca aaccgcacca aaccgttttg 9240cgcggtttgg gttgggttgg tttgggaaaa agtgcggtgc ggtttgggtt ggaaaatttt 9300caaaccgtat atttgcggtt tgggttgggt tacatcccaa accgcacaaa cccaaaccgc 9360gaacacccct attaataaga tacatattaa ttcgaatttg acaagatcca catgactatg 9420tttttattcg cgtataaacc acaaaagaag gttcaagtaa aatttgtgta tggtgtaaca 9480tgtcaatcaa agaacggagg taatatttgt caagacactt tagtcacttc taaattccta 9540taaacaaaga aatatggaag aaaactggtg atgaaaattg aaaaggtggg tataataaga 9600gagacacaat tctaaaataa gaaaatatta ataataaaat aataagttac gataggcctc 9660atgtttgaaa acggaaaaaa taaggagata gttcgtgtaa aaaggaggga gtaaagggta 9720atgcatactt tgtattgcaa gcttagtttt aaaaggcata agacgcaaag cgcatcgagg 9780cacaagacga aggcgcatgc atctcgtagt tgaggcgtgt aatgatttta cttcacaacc 9840acctgagcaa cccaatacag aacgaccaca agaaaaatag aaagaaagga aatgattttg 9900attgatcagc agaaaataca gagcattcga gaggctcagt ctctcccaag gactacaaga 9960tactactaaa tttcacaccc ccttcagtcc ccttacaccc ttatttatac tacttctgct 10020ctcctatttt aacggctact gacattctct gagctggcct gctattcctc tttttgtgct 10080gacatttctg aatattctgt ggtagtggct ccattctcac atttggacag gtttacccct 10140ccatttcttt cgtgcatacg tcagccacgg tttggggatt gaattcatta cattaccctg 10200cccccaaaga ctcaccttgt cctcaaggtg gaaggaaggg aaacgttctt gcaccaaatc 10260tgcatcttcc cacgtggctt caaaaggtgc taagtccttc catttcagta gcacttcagt 10320ctgcgtatgc ctccctctct gtgtctgacg tacgtccaat aattcttcag gttccacaac 10380tagttccaag tctgctgcta gttgagttgg tacagtggtt gctgcctggg catctccaat 10440tgctcgtttg agctgggata cgtgaaatat aggatgtatt ttactggtgc ctggtaactg 10500gagtttatag gcgaccttgc ccaccttttg cagaacaggg aatgggccat agaagcgggc 10560tgccagcttc tcaaatggtc gcttggccaa ggattgttga cggtatggct ggagctttaa 10620gtaaaccaga tcccccactt caaaggactc atcgcgcctt cttgtgtcag cataggcttt 10680catcttttgc tgggagcgta gtagatgaaa gcgtaaatca tcgaggatgg catcccgttc 10740ttgcaacact tcctctaagt tatctactgg cgtttgccct ctgcctactc gccacaagtg 10800tgtgggtcac gcccgtacaa caccctgaat ggagtcagct tagtagacat gtggggagag 10860gtgttgtgtg agtattcagc ccaagggagc cactttgccc aagtcttcgg gtgccccgcc 10920acgaaacatc tcagatatgt ctcaagtcct ttgttcacaa tctcagtttg tccatcggtt 10980tgcgggtgat aggcggtgct tctccttagt gttgtccctt ggagtcgaaa caactctttc 11040caaaaagtac tcagaaaaat tcgatcccta tccgaaacga ttgatgccgg aaacccatga 11100agttttacaa cctccctgat gaaagcttca gccacttgtg agagcactaa aaggatgacg 11160aagcccaatg aaatgcgcat atttcgataa acggtccacc actactaaga tcgtgtccac 11220ccccttggac aagggcaatc cttctatgaa atcaagagtg atatcctccc aaacctgagc 11280tggaatggct aagggctgca gtaggcctgc tggcttttgt tgagagctct tatgctgttg 11340acaaatgcta catcgctgca cgtacaatgc cacgtgcttc ctcataccta tccaatacca 11400ctcagccgcc aacctaaggt acgtttttac ttcacctgca tgtcctcctt ctggggaatc 11460atggtaagct atcatcaact taggaatgat gacggaagtg ttgggaatta ccattcgccc 11520cttataccgc agcttgccat cctccaccgt gaaccccaca agtggtttat ctccctgcgc 11580cacttcttcc ctgagccttt taaggaacca atcctcctct acctcttttt ggagctctgc 11640ccagtccact ccttgggttg tgattatggt ccctagctcc atttcaccta cagtttttct 11700agaaagcgca tccgcaacct tgttggttgc ccccggtttg tagtgtattt caaagtcaaa 11760cccaattaat ttgcttaccc atttctgaaa atcagccccc acttcccgtt gttgtgtgat 11820gaaacgcaaa ctttgttgat ccgtatggat cacaaatctt ctccccaaaa ggtaatgttt 11880ccatttctgg accgcaaggc atatggcaat taattccttc tcataaacgg acttgtgttg 11940tgctctcggt ccaaggagct tgctgtagaa tgcaatgggc ctgccctctt gcattaggac 12000tgcccccacc ccatacccag acgcatccgt ctcaactacg aaaggcttat ggaaatcggg 12060catagcaaga accggtggct gggtcatagc ttcctttaag tgagagaaag ctgaagtagc 12120tttttcggac cagccaaagg agtccttacg caattgctcg gtaaggggct gggcaatttg 12180cgcgtattgc ctgataaact tgcgataata cccggtcagc cctaaaaatc ctcgaagctc 12240cctcaaattc ttgggaactt cccactccac catggccctt atcttctcca tgtctactgc 12300caccccatgc tgcgaaattt acatgcccca agtaggccac tgtcttcctc cccaagtcac 12360atttcttctg gttagcgaac agtttgtgca atgctaacag ctgcaacacc aatcccatgt 12420gtcgtgcgtg gtcctctttg gtcttactgt agaccagaat gtcgtcgaag aagaccagca 12480caaacttcct cagatatgga cggaaaacgt tattcatgag tgactgaaaa gttgctgggg 12540cattggtgag cccaaagggc attacgagaa attcgtaatg tccttcatgg gtgcgaaaag 12600cagtcttatg ggtatcctcc gggcgaacta aaatttgatg gtatccggcc ttaaggtcga 12660gtttagagaa gatggtagcg ccatgtaact cgtctagtag ctcatcaatg accggtatcg 12720gatacttatc cggaaccgtc tccttgttca aagcccgata atcgacacaa aacctccaag 12780aaccatcttt tttcttcacc aataatacgg ggcttgaaaa tggactagtt gagggcttga 12840tgatgcctgc ctccagcatc tctcggatga gtctctcgat ttcgtctttt tgaaattgag 12900ggtaacggta tggcctaacc cccaccggat tactgccttc cttcaacgtg attgcatgct 12960catgccccct ctttggtggc aggcccaccg gagtatcaaa aacttccgca aactgactaa 13020ttaccttctg taaaaattcg ggtacttctt gtgcctcctt caactccgct tctccccttt 13080tcccatcatc ctcaatctgg ttgagctcca agagaaaacc ccctttttct tttcggattg 13140cctttatcat ggctctaagt gagattttag atcttgctaa ggaagggtcc cctctcaatg 13200tcaccactct gccctccact tcaaactgca taacctgagt tttccagttg gtaatcactg 13260accccaattt ctcgagccac tgcactccta atattaaatc tgagttaccc aggccgagag 13320gtaaaaaatc ctctgttact tcgatttccc ccagctttaa agtcacccct tgacacaccc 13380cagtaccatg gacagcttca ccattcccta aagacactcc aaatccccct gcatctgaga 13440tgaccaactc aagttcctca acagttaaca aggaaataaa attgtgagtg gcacccgggt 13500caatcatgac caccacctct cttcctttaa tttttctagt gattttcatc gttttaggac 13560tcatcaaacc aatcacagag ttgagagata cctcagtagg aagttccggt ggtggttcgg

13620acggtggtgc acgagctgcg tcgctcacct cttcggtttc ttcctcctcg tcatgcatca 13680gaatcacgct gatctctttt ctccggcaga tgtggccggc ggtccactta tcgtcacatt 13740tatagcacaa cccttttgct ctcttctctt gatattcttt ctcggaaagt cgcttgaact 13800ctacagattt ttttccttgc cccccagcaa ttggatacgt gttcaaggtg ttggaatttc 13860cccctgggtt ttgggcccac attttgctgg caggtgggtt gaggctggtc gttggattga 13920aggaagcccc tacactcctt gtcattcccc ctctgttata aatcgagtaa ggcccattct 13980tagttggccc acttctttta taacccacaa tcctattcct ttcctcaatt cggcctgcta 14040gttccattgc ttgctctagg tccataggat tgagtaacct gacctccact ttgatatcct 14100cttccagccc attaatgaac tgacccatga gtatttcttc tggtactcta ctcaacggtg 14160ccgccttctc aataaaagtg cgtcgatact catccaccgt ggtggtttgc tttgtggcca 14220accaccgttc ccacaatgaa ccatagtggg ttggtcgaaa ctgacggagg aggtactcct 14280tcagatctgc ccaccacctt atcggccgcc ttttattctc ccactggtac cacctgaggg 14340catccccctc tatagacaca accgccgcct ccagggcttc actgctactc aggccataaa 14400acgaaaaata tcgctcggct ctaaggatcc acccatccgg atcggaccca tggaaaatgg 14460gcatctctaa ctttcgatat ttccagttcc ccccggaagt cgaacctcct ggccctccac 14520ctgagccgcc atccccatac gatcggcccc cgagctggaa acctccatat tcgtcccctt 14580ctcggccccc cagatttatc aggtcaggag ccctccgttc cggcggtcgg gggtgtccag 14640tgacggtttc gggtgtctcc cgcgagggtt gtggcaaggt tgctcggatt tcaacctgaa 14700atttcctctg ttcagcacgc aacccttgaa tcgtcccatc ctgtgtctcc cttgaccggt 14760taatccggtc ctccagccta acggccaaga cctccatctc ctcgcgactc ttcctcgccg 14820cctcattctg gccttccaag atttgagcgg tcaacgattc ccccatggca ttaatggcag 14880attccaccgc cctggacacc atggtggcca ctgacccttc gagggctgcc attctttctt 14940ctaatgaatc caccctctgc acttcgtttc ttggtgccat ggatcgatgc tctgatacca 15000agtgtaatga ttttacttac ttcacaacca cctgagcaac ccaatacaga acgaccacaa 15060gaaaaataga aagaaaggaa atgattttga ttgatcagca gaaaatacag agcattcgag 15120aggctcagtc tctcccaagg actacaagat actactaaat ttcacacccc cttcagtccc 15180cttacaccct tatttatact acttctgctc tcctatttta acggctactg acattctctg 15240agctggcctg ctattcctct ttttgtgctg acatttctga atattctgtg gtagtggctc 15300cattctcaca tttggacagg tttacccctc catttctttc gtgcatacgt cagccacggt 15360ttggggattg aattcattac aaggcgcacc tttggtacca agagacttag acatcaaata 15420aagaagcatt gtaatactta gaataaacat tgtttttata ttctaaaaca catattttct 15480ctaagacacc tagtaatctc atagtggatg tcatctagtt taggtggtaa ggttttattg 15540agttggtact caagacctca gacttagagg tggcaaacgg atcatacggg tcgggtgaaa 15600atgagtcggg tcataatcgg gtcacctttg tgtccaggtt acggtcaggt cgagttcgtt 15660cgggtacgag ttcatattga gtccatgagg tttcatgtca tatcgggtcg ggttagattg 15720gatttacaat ttcgcaaata aataaaacgc atataatact aaagagagta aattaaataa 15780ttaacggaca ctagctaaat catatattag tattttatga tgtattttcc ttaaatttat 15840ttaaaaaata actaatatga caatttttcg ggccgggttc gggttgtggt catcattatc 15900gggtcaattt agtatcgggt aggctcgggt tcatgtcata ttcgggtcta ttttaattcg 15960agtcgggtta tttcggattt aagctctatt tcgggtcagt attttcgatg aagaacgggt 16020ttcggatcgg gtcaccggat acggatctat tttgccgagt cagactgctt ttcaaaccta 16080ataatctcag tttttccacc tattcagatt tgcctatgat ctctatttag ataaatgagt 16140acaatattgt gtctatccat gaaaatgaat atctcacaat gtaaaaggat atctctaaat 16200ttcactaatc actctatctg ttttgaataa taatattcta ttttattgca tgtagtaaag 16260atcgagtatt tagtgagatt tggagaaaag aggaggctag agagaaacta ggatttagag 16320aggagaaggg ggctctgtaa cacatacaag atagatactc ttttacacta acttttcaag 16380atactcaaca tataaaatca gcatcatctt ccaaacaaca actttaagcc acccatgaat 16440cttaattaga taataaaaca taatcgtgaa tcatctatcc tttgtttggg gggatcctaa 16500agcaattgag gaaaagcttt gatgcaaata tcaattgtgt aaaaaagcaa gtattcgttc 16560gtgatgttgc tatactaggt tattttttgg atccaaaagt cattcctact agaatcattt 16620aggaaaattg tcagtatgaa ttttaaattc aggttataac caaagataat tgaaaattgt 16680caaacttttc aaataattcc gaaataaaca tgtttgtaac atggataaac ttttcattgc 16740ttttcaaata attccaaaat aaacatgtta taacatagat aaccttttca gataattcca 16800aaataaacat gttgtatcat ggataaactt ttcattgttt ctagtcactt aaaattctaa 16860aaaaatcttt cctccctact gttactctct ctagcaccaa atctatcaca tgagaaggca 16920gaggttttca aaataaaccg ttacttaatt tggtacttat ttcttgatcg gtgttcatat 16980catatgagtt cctactctat atctctctac tcttctaaat ccttgtgtca cttcctgtgt 17040ttcataaata aaaaggagga agtattagtt ttgaaacgaa aggagtatgg tgcatacatt 17100gatagaaaaa agaagttatt tgtccttatt tcactcatat aacaacacca aattctgtat 17160tgttatcaca aaataaaact tggattatct ttgtttcata gcccaaattt agaattagtt 17220tgtcagattt ccaatcatct aattacaata ttagagctag acctaggaca aaaggtgggt 17280ttggctactt ggtaatagct atgtctagtg ctaggatatg tcattgtcgt agaaccatgt 17340tatggacatc ttaagaaaca aggttaacct aattggttgg agatcctact ttcactttta 17400taataaagtt tcgattcttg cctatttgta aagtagaatt cctaaatttc ccttcactga 17460tatttatctt aacataaaaa aatgttataa acattgggat tgtatataag tcaaaataaa 17520ttgacaatct tggtaacaac taagttaaca ttaattttat aagtaaatga ttaatcccaa 17580tataatctct tatttagtaa atgagacaaa cttgtacacc ttcgtgttag actcgttaat 17640gttcgctaac aattcattca gtagtcaaca gcattttaaa tttgaaataa gtgttcttgt 17700ggtttttgag agatcaagca agaaaacatg tctctcccct ttgaccaact aattgggatt 17760aagaatacta gttttaagat tttaagaatg agttatagtc tttcttagac cgctacaatc 17820cccttgttga tatgaaccag atatattttg tgttcaaata gtagatcaat gcattgttga 17880taatcctttg ttaatgtact tgttgatctt attttgtact tttggtagat gcgctatact 17940ttctttcgat tgctcatttt gaactcttaa ctacatatgt tagtttaagt agatgattta 18000gaattgctat ttcaatcttc aataagcaat ttaagttgtc aaaccttgtt tcacatcatt 18060agggtgaaag ttatttggat aaagacctat atctaattca atccaaagca aattagtaat 18120gcggattgga ctcaaactat gtttagattg gattcgaatt gagtttcttt tctttttctt 18180aaaaaaattg gattttccga tcgaattgag ggtgattaga tccaaaataa ccgaatagta 18240gataggattt gtgttgtata ttagaattgg gcttaaggat ttccatttta acaaaaaaac 18300caaatggtcc gactatcaaa aactataatt tgatagtcat gcctatcgaa aactttattt 18360tcattctcgc acctaattat gggcttgtat aaattagttc tactatcgaa aactaatttt 18420gatgctcgtg cctaatttaa attttcgaaa aaatgaagtt aagaaaattg gatatttcgg 18480attggatcca atatatcttg tgaattatta atttggatta gtttggactc aaattcttat 18540tggattggat tcaaattaaa agattaaaat tcaaattttg ttcgaatcaa attggagtag 18600gcttaagttt aaatcataca ccgaactttc accactaccc atcatgctta agcttctaat 18660gtaagagagt gtttgggagt tgagctcgaa caactaaatt tctaaaagaa ccaagttcaa 18720acaagaaatc taaaagctcg attaaacttg agtcaagctc aaacacctat attccttatt 18780ggagcttgac tgaagattga acacttattc cttattaagc tttacgctaa aacattgctc 18840gactcaactc ttctacatcc ctatagttca aaagaaatag ttgtgggctg tggtgctctt 18900gtagaccaac gcactagttt aacaaagcta agtgcctgac tgcaattcca tacacattac 18960gatcaccatg acctagtttc agctcacact ttggaagtct aatttgaact tgttctctac 19020ctccaattca ttgtggggta ggaggcgata gttaagggat caaaatctta tgatataact 19080tgcataggct atgccactat ataatgcgtc ttgtgtccca tattagttta atcaaattga 19140aatgttttac catttatatc ttcaattatg tatggatact aatatttgat ttgacgtttg 19200atatgatatt aaatgtggac tgttattctt gatgtgcttg agaagctttt ttggggccag 19260ttagaaacta tattcctttt atggtcctaa ctaggttgtt gttggtgtgt tcccaaataa 19320cagcatggaa aggatactcg agcgctatga aagacactca tatgcagaga gacaactgac 19380tgctccagat cctggatccc atgtaatcca gctaggcaac tatcttttct aagcatttaa 19440atcgttgaga tttcaatttt aaatgtgttt taactgataa ttcatgcatt atatgcttag 19500gtaagtttga ctctggaaca cgcaaaactt aaggctaggc tggacattct ttagaaaaat 19560caaaggtaat aagatccaga ccaaatataa tttgtataat aaccacctta tgaggaaaat 19620ttaagatcct tgataatttc aggcattaca tgggagaaga acttgatacc ttgagtctca 19680aggagcttca gaatttagag catcaaattg acagtgctct taaacacatc aggtcaaaga 19740aggtagtttc acagttgcat tagatcatct tatggatcaa ttggatcact tgtttgtatt 19800ttagcgttgc tcaacacggt cgtctaatat agtgtgcaaa acgacctaca gggcaacacc 19860ttttataggg ctcgaaaata cgaaaaatta aatgtttgtt ttagtcatat tgttcaaacc 19920caagctttat cttgtcaaaa atattttata atgattattt tttagaatac attatttaca 19980tttttgcaat ttatgcataa tacttctaag gtccaacttt ataattgaaa tagaagtcct 20040taaattttaa agacgacctt gaggaaacct aatttcttct catatataat taaatcaatt 20100attctacaag ttagtagaac aaatactaca ataacaacaa tattgaagcc ctaatctcag 20160taggattgga ttgattgtat gaagtcttat tagtggccgt taaatgtttc ttgtaggtca 20220agatgacatg gctcatatag taaggttact tgactaaaag acgaggattt gtttcgactt 20280agattttaac aagtttccct catttgttaa cacctaagcg tactaaatca aattctaggt 20340tttactcact caaatttccg atttaggaag ggcttgagga tagttgtatt atcgtaactg 20400actaatcaaa ggagcctctc ttagatcagg tttcacttgc caattctaac aacttgtttg 20460gtaaaaggaa tttggaatga aaagaaagga attgaaaaga aacattctac ttttcaatgt 20520ttcattcaaa aataacattt taagtgatag gaaatggaaa gaagtgaaac gaaagcctct 20580ttacaaaatt atcatttttc tacccccccc cccccccaaa aaaaaaaaaa aaaataagta 20640gtaagtagta gaagaagaaa taaataacta acaagagtag taagttttta cgttttcttt 20700ctnnnnnnnn nnnnnnnnnn nnattcaaat cagaactgaa tagtcataac cggaagatta 20760gtttctctct agcgtgacta gggtttgagt aaaaagagaa aacttaaatc aaacatggga 20820tattaaggtt ttttttcctt tcttcagttc ttttctcttc ccaatccttt cctaaaaatg 20880aaccaaacag gctgcaaggt tttcacttgc ttaacacaag atttattttt aaaaataatt 20940acactccaaa cttttaagct taaaaccaat tttaattcaa atcagaactg aatagtcata 21000actggaagat tagtttctct ctagcgtgac tagggtttga gtgaaaaagt ctagggtttc 21060atgtcattct tcttgcttcg agtcccttct tgggattgtt gttagccatt atggctaccg 21120aaatcgttat taaatgtcta aatcttagaa ttactgctga agaaaacaac ttggtgtttc 21180tcgaagatgt tgatgataac tcgcagcacc atacgctcgc actggcgatt gttggaaagg 21240ttctttcgtc aagaccatac aatttcgagg cacttaaatg aaccttaaac tagatatggg 21300tgatatccaa aggagcccta cttcacccta ttgaaaacgg actttttgtg gtacaatttg 21360cgacaattaa ggaccgatct aaggttctag tcagcagacc atggaccttc gatataaacc 21420ttgttctctt agatgctatt gaagggggta ctcaatcttg acccattgcc cgttttggac 21480tcgcttgtat aaccttccta tggactgccg atatgagaag ttcatcaaaa actattgttg 21540gtgtattggg ggaggtattg gaagttgatt ttgacaggat tgtttgggat aaatctgcaa 21600gagtaaaggt gaagattgac attacaaaat cgttttgtcg tgtgcagatg atcaagacta 21660acaggggtga ggctgtgatg atcaatgtta agtatgaaag acttcctaca atttgttatg 21720tgtggaattc tggccatatt gaaagagatt gtgtgaagac ccaggaagaa gagaaacaag 21780tggagagaca atagggggtc ttggaggcct ctccgcgtag gggacgatta aagatggtga 21840aagagtcgaa agccttcctt cagtgtgctc gtacactcca ctttaataac aaggaagaag 21900taaggggtga ggaaccacgg gattatgtgg agccgagggg ttattgtcgg ctatcttagg 21960gggtaaaact ttggtggtcc aggagatagt ggacggctct aaggatgcca tcgaggaagt 22020tcgtgctgaa ggtgcaccac tctagccccc ttgtaccctt tgggtaatgc catgctacct 22080tttacttttg ctgttgggag tgctaatcct actccctccc accgaaaagt taaaattaaa 22140aacaaggcaa gggttcaggg tgttttgaac caagttaatg ttgtgggtgt tggggggttg 22200gctaataatg ggggttgtga gaaaaggata ttccccaacc cgatggtgtt agaaaaagaa 22260aaggggttca atgaagaggg tttaagatag caaaacgaga ggattgtatg taacctatca 22320gtagggaggt aactattgag gtggaggtgg gcgagaccca accccgcccg acattatgaa 22380tatcctatgt tgcaactgtt ggggattggg caacccccgg gaagttcgga tgcttcgtag 22440gtggagcaat agtgctacac tgagttcggt ttttatttct aaaactatga ttagtggtcg 22500tgatgtggaa agggtgcaaa gcgggtaggg ttttgattgg gcaattgggg tggatagcgt 22560tggaacttca agagtttggt gcatttattg gaaagctggg gaagtggact ttactctagt 22620ctctctatca agtcatcata tttgtgggaa tgtgaagctt gttgatggga aggtatgatg 22680cttagtgagt atttatggtt gggcggatac aattcaaaag tataaaacat gggagcttat 22740gcaatccttt cactcatatc atgggccgat attgtttggt tgggacttca atgagatttt 22800gacaatcgga gaaattgaag gagggtccga aactcaatga agtaacatgc ataattttct 22860agaaacttta gatgacatga agcttaggga ccttggctat tcgggaactt ggtatacata 22920agagagaggc tttaagccac ggaagagaat gagggagaaa cttgatcatt ttgttgcatc 22980ttcatcatgg tgtgacttct ttccgaaagc tacagttgag cacttgatgc gctacaaatc 23040ggaccacact cctattttgg ttcgccttgt aggccatcag tgaagacata agaagaaaaa 23100gacgtagttt tgttttgaga ctgcttgggt gcttgaggaa ggttgtgagg cccaatgggt 23160gagtcatggg ccgggtttac tcgcgaggta tttatcgagc gctttaaagc cgtggaaggt 23220gggttcaaag caaggagtga tgggtctctt agtaatctgg gcccgcgtgt gagggagatt 23280gaggaggcca ttatagatgg gaggcagcga agcagataag gactatgagg ctctatgaga 23340ctcctctccc acgaaagtta gacgaggtgt tggacaagca ggagacgttt tggtttttga 23400ggtctcgtgt gagttagata aaggatggtg atcgtaatac acaatatttc caccacaaag 23460cttcccaaca caaacgtcgc aactacatag cggggatgta tgataataaa ggggtgtggc 23520aagataacga agaggatatt gaagggaata tttcagagta ttaccaaacc tcgttcggtt 23580cgtgctcccc ctctaggaag aacgtcgcgg ttgtccttga ggttgtgagc ccggtgataa 23640ctgatgatat gaatatggcg gttatgaaat cttacactaa agatgaggtg tgggaagcac 23700taaaccacat gaagcctaac ggaatgcatg ccatccttta tagaggttct ggaatacctt 23760ggagatgata ttacatctgt cattttaggt attattcatg gcacccgacc ccagatgttt 23820ttaacaagac taatattgtg ctcattccta aagtcaaatc cccaaatctt gtttctgagt 23880ttcgcccgat tagcctctgt gatgttatct ataaacttgc ctcaaaagta cttgctaaca 23940gattaaaaca ggtttgcctg acattgttta tgataaccag agtgcatttg tgtccggaag 24000ctatattacg aacaatgctt tgatttctct tgaattattt gactctatga aaaaatgata 24060cagagctagg aaaggttttg tgtcgatgaa attggatatg agtaaagcct ataaaagagt 24120tgagtggtgt tttttcagta gtgtgttgga gaagttggat tttgctgaat catgagtgaa 24180tgttgttatg agatgtgtgt cttttgtgca gtactctttt gtggttaatg ataatatatg 24240tggagctctg acaccctcaa gggggctttg acagggagac cctatatccc cgtatttgtt 24300tatacttgtt gcagataccg ttttagctct tcttagcaag gcattcaaca atgcgtggct 24360atacttgata ttctcaacaa atatgaggca gcatcaggct agaaaataaa tattgacaag 24420tcaggaatct ctttcaataa aagatttgac gtattttatg gccatgaaac aagttgagaa 24480gcatcagaaa gacttggtat cccaactttg gctaggagtt cgaaaaaagt catatttgct 24540gacattcaag agcgaatttg gaagaagctg cacggatgga gagaaaaact tctcgcgggc 24600ttgaaaagaa actctcttaa aagttgtggt tcaagcaatt ctaacctatt tggtgggcgt 24660ttacagattc ctaaccagta ttatccaggc cattcatttg gccatggtaa agttttggtg 24720ggggtcgaaa agggcccaca attcgatgca tctgggggga tatgtgctca ccaaaatgtt 24780taaggagcct tagctttaaa gacttagggg tgttcaatga acctaaacta aggaggaatg 24840cgtggcattt gattcctgct ggtgagtccc tttcgggtcg agtgttctcg gccaagtact 24900attcgaagtc aacctttttg gactcatttc taggtccggt aggtagcttc tcttggaaga 24960gtatttgggg ggccaaggca ttagttaagg gtgttttatg gtgcgtaggc aatggcagac 25020aaatcaacat atggcgtgac tcgcgggtgt tgaatggtga tagtaggttc atccccggag 25080agcgcgtttc aggccttgag gatgtttgtg atctaataga ttttgcacaa tggagtgcga 25140tgtggacctt gtcacgattg cttcaatgaa gatgatgctc aagccatttt agtcatacct 25200ctaagtaagc gccttctgaa ggacatggtc tcttgggctt tcactaagga tgaatttttt 25260ttgtaaaaac aacctatatg gccggttggt cgaggaattt gaatttgttt cacaaagcat 25320ggctgcaaat ctggggcctt aacgtgtctc cgaaggtctg ccacttcctt tggcgtttat 25380gctcggtacc cttcctgttc gagctctttt aaaacgacgc cacataactg atgatgattc 25440atgtcctttg tctaaaggag cccggaaagc atatcacacg cgttgttcta ttgcccatat 25500gtagccgaag catgggagag tgcgggcctc acaaattgtt tgcctttgtt tgatggggct 25560ggtatgcttg atgcgtgggg ggagtgggaa acaatcgatg actagtccct tgtaagactt 25620agcttcttgg cttatcactt gtggtttagg cgaaataaat gtgtttttga aggggtggtg 25680agagcgaatg agagtgttgt ggaatatgcc actaaagcta ctgttgatca tggtttgtat 25740agtgcccgca tttatggtgg gtcgaaggct accgcatcca aaagctcgaa ggtatgggtt 25800ccccctccag cttgtcgtac gatctaaagg ttgatgcatc agtggggaat ggtggatggg 25860tggggctagg agtaatcgcc tgaaactaga aaggggaggt gctcgtggct gcaactagga 25920gggtcagagc ttgtggcccg tggaaatggc tgaagggaag gctctttgtc ttgctcttag 25980gcttgcctcg ctcatacaac ttgcaagaag tgatcgtgga gtttgactgt caatcttggt 26040gaaccatctc tccaagggtg ctatttactt tgcattttta agtcaaagct tgaaccttga 26100taaaaaaatc ccgttcgaca tgaaaagtgc cttgattttg cgggtttggg agtgccttat 26160tgattctggg gtttgatttg taacaccttt agtaaaaaca tgtaagctaa ctgtaaaacg 26220aacattaatc aaactaggat atgtaaaatt cctaaatcaa gaagaatttc cacttgtgct 26280gaatttgtcc accttgcatg acacccaata aaagcccatg tctcctagaa ccccttatgc 26340cgccttattc atcttttctc aagttgagtt ggagtcctct atggtccact cgacttcttt 26400agcacactct cggtaaaaac ttttaatatt attttatttt agactccacc atcttgacat 26460ttattccttc ttaaacttgc ttcacacaaa catctaacac tagaattcta tatagaatag 26520cttgaatctc tcttaggata accttatagt aaatgcaact acgcctatcc ttaaaccttt 26580ctaagaggag ctttatcgta tttacattcg cttcactttg aaacgtcgct aagtgtatgt 26640tgcactttcc aaaccatgtg ttagctaaga ccaagttata tgactgcata acctaaatag 26700tcttcctcga gaaaattcac tagttggatc ggaagagttt gtgtaaatct atggcggcgc 26760gggactgggc cttcacgatt tcaagtgttt taatgcagct cttctaggta aacaagtttc 26820ctaatagcgt ggtgactcaa atattgagga cttgttgtta tactaatgct attcctggcg 26880gcacttaagg ggtgaaatag gtggtacaaa ggagtgcttg atggcgtgtg ggtagtagtt 26940gaatatatca gtatgatcaa gtccatggat ccctcgtact tattcgtgca agattatttt 27000tccacgaggc aaagcgagcg agaatcttaa ggtttatgat catattcatc ttgtacgtgc 27060taagggtaat gtccctttca ataatgagct atttctcctt ttgagcaaga gcgtatctta 27120agcattcctc gtagttctcg tctccccaac gatgttttat gttggaatct gaatttggag 27180aaagacggag acttttcgtt cggtctatcg agccattctt ttgagttgga tggcgagagc 27240gtgatttcat cgtcaatacg ctctaattta tggagtataa tatggcagga tagtaccttt 27300caacgtgtta agcttttatc tgcattgtcg acacaaaggg gattgagtaa gcctgtgccg 27360agtatggaac cattgtgtaa tctttatgcg ttggaggatc aatggagcta cacttcttac 27420gagactttgt tattggaagg gcttatatgg gatccaacta gggtagtcaa aacattggtc 27480ggggctgcgc tgcaattttg gggacttggc accggcgttt ttggagtagc tccctcatgt 27540ggaacatagg cttttgatga cgatatacta ggctagatgg aatataagag agaggtgttt 27600gtttgaggag gaggtttgtg atccctatca aaccacatgc ataatctcat ggcgtagctt 27660catgtggaac actgacatat tgcatgtgta gggttaggtg cttgcctagg acaccacatg 27720ggaagagctc cgttaagctt aatgtggatg gagggtgtgt ggaagggttg ggtgcgtcca 27780ctggagtggt gattaggggg atggatgaaa aagcgttgta gttgcaacat agaaaggtgg 27840aggactgcga ggaaccgtta aaaaggctat attttatggt gttcatttgg ttgtggaagt 27900cgatttttga aatatggttg ttggaagtga ctatcttcac ctcgttgaag caacttcttc 27960aaaagtggaa ggcaaaaata gcttccatgt tattgttgat gacattgttc atggtagtgg 28020tatgttaaat acttcgtctt gtagttttgt tcgtagggat gggaataggg tttctcacga 28080actcccccat ggaaatatca taatggctca tgctcatagg taaatgaata agctaacaaa 28140attgttcatc tttgcagaac caactcatgc atgaatcgat ttctcagctt cagcgaaagg 28200tagacagctc tagagagagc atctagtctc aaaaatacca tgagattctg tagtgtcctg 28260acatgtttta tatgacagga caaagcgtta aaggagcaca acaacttgct atccaagaag 28320gtataagttc agcaagattg tttagtaaca ttgttaatct tgctgattgc tttgaaacat 28380gtcttgctat ggttaacaat gttgactgaa ccaaaatagg tgaaggagag ggagaaggtg 28440ctggctcagc aggcagaatt ggatcagcaa aatcatgaca ataactcatc tggctttgtg 28500atgtctcaag ctttgccctc actgaataca gggtcagtcc tcaataacct ctaatcattt 28560ttccaagatc caaagtaaac atggtttcat aatttaatta agattttttt gaaccatgtc 28620tccatacaac cttactagga ctaatactac taatttaaga ccccaacgat aaacaacaat

28680aattagccat atctggctag caccttttgg acaacacacc acatgagact cttggccaac 28740ttctttgatt tccttcagtc tgatagatat gaatatcttc tgaagagctc tttggttcat 28800aattattgat ttagaaaaga attcagcaag gtgagtcatt tggtaacctt aaggtcatta 28860tgggggtact aaatcaaagt gaagatatat ttaggtggca tcagaagaga tgatatagat 28920aggttgtatc ctgtcgatag gttatttgga tatgtatcaa aagtttcttt tataatatat 28980ctatactgat tggttgatgt atcaaatatc cctacagatt gtgaaaaaat cccctacaga 29040ttgtgaaaat atccctagaa cctgtgatga tataagatgt gctccgcatg ctttattgaa 29100cataatgtat tcaattcttg aaatgcagag gaacaagcag cagtgcagtg gaagatgaag 29160caacacaacc accaaatcta aacagcaact ctgcacaaat accgtcctgg atgcttcaac 29220acatccaaga gcagtaa 2923741256PRTArabidopsis thaliana 41Met Gly Arg Gly Arg Val Gln Leu Lys Arg Ile Glu Asn Lys Ile Asn1 5 10 15Arg Gln Val Thr Phe Ser Lys Arg Arg Ala Gly Leu Leu Lys Lys Ala 20 25 30His Glu Ile Ser Val Leu Cys Asp Ala Glu Val Ala Leu Val Val Phe 35 40 45Ser His Lys Gly Lys Leu Phe Glu Tyr Ser Thr Asp Ser Cys Met Glu 50 55 60Lys Ile Leu Glu Arg Tyr Glu Arg Tyr Ser Tyr Ala Glu Arg Gln Leu65 70 75 80Ile Ala Pro Glu Ser Asp Val Asn Thr Asn Trp Ser Met Glu Tyr Asn 85 90 95Arg Leu Lys Ala Lys Ile Glu Leu Leu Glu Arg Asn Gln Arg His Tyr 100 105 110Leu Gly Glu Asp Leu Gln Ala Met Ser Pro Lys Glu Leu Gln Asn Leu 115 120 125Glu Gln Gln Leu Asp Thr Ala Leu Lys His Ile Arg Thr Arg Lys Asn 130 135 140Gln Leu Met Tyr Glu Ser Ile Asn Glu Leu Gln Lys Lys Glu Lys Ala145 150 155 160Ile Gln Glu Gln Asn Ser Met Leu Ser Lys Gln Ile Lys Glu Arg Glu 165 170 175Lys Ile Leu Arg Ala Gln Gln Glu Gln Trp Asp Gln Gln Asn Gln Gly 180 185 190His Asn Met Pro Pro Pro Leu Pro Pro Gln Gln His Gln Ile Gln His 195 200 205Pro Tyr Met Leu Ser His Gln Pro Ser Pro Phe Leu Asn Met Gly Gly 210 215 220Leu Tyr Gln Glu Asp Asp Pro Met Ala Met Arg Arg Asn Asp Leu Glu225 230 235 240Leu Thr Leu Glu Pro Val Tyr Asn Cys Asn Leu Gly Cys Phe Ala Ala 245 250 25542242PRTArabidopsis thaliana 42Met Gly Arg Gly Arg Val Gln Leu Lys Arg Ile Glu Asn Lys Ile Asn1 5 10 15Arg Gln Val Thr Phe Ser Lys Arg Arg Ser Gly Leu Leu Lys Lys Ala 20 25 30His Glu Ile Ser Val Leu Cys Asp Ala Glu Val Ala Leu Ile Val Phe 35 40 45Ser Ser Lys Gly Lys Leu Phe Glu Tyr Ser Thr Asp Ser Cys Met Glu 50 55 60Arg Ile Leu Glu Arg Tyr Asp Arg Tyr Leu Tyr Ser Asp Lys Gln Leu65 70 75 80Val Gly Arg Asp Val Ser Gln Ser Glu Asn Trp Val Leu Glu His Ala 85 90 95Lys Leu Lys Ala Arg Val Glu Val Leu Glu Lys Asn Lys Arg Asn Phe 100 105 110Met Gly Glu Asp Leu Asp Ser Leu Ser Leu Lys Glu Leu Gln Ser Leu 115 120 125Glu His Gln Leu Asp Ala Ala Ile Lys Ser Ile Arg Ser Arg Lys Asn 130 135 140Gln Ala Met Phe Glu Ser Ile Ser Ala Leu Gln Lys Lys Asp Lys Ala145 150 155 160Leu Gln Asp His Asn Asn Ser Leu Leu Lys Lys Ile Lys Glu Arg Glu 165 170 175Lys Lys Thr Gly Gln Gln Glu Gly Gln Leu Val Gln Cys Ser Asn Ser 180 185 190Ser Ser Val Leu Leu Pro Gln Tyr Cys Val Thr Ser Ser Arg Asp Gly 195 200 205Phe Val Glu Arg Val Gly Gly Glu Asn Gly Gly Ala Ser Ser Leu Thr 210 215 220Glu Pro Asn Ser Leu Leu Pro Ala Trp Met Leu Arg Pro Thr Thr Thr225 230 235 240Asn Glu

Claims

1-15. (canceled)

16. A method for producing a Beta vulgaris plant, where the bolting and flowering is inhibited after vernalization, comprising the following steps: (I) mutagenizing one or more parts of a Beta vulgaris plant and subsequently regenerating one or more Beta vulgaris plants from one or more parts to yield a regenerated plant, or mutagenizing on or more Beta vulgaris plants to yield a mutagenized plant; (II) identifying a regenerated or mutagenized plant of (I) that exhibits one or more mutations in an endogenous DNA sequence; (III) generating a Beta vulgaris plant in which the one or more mutations in the endogenous DNA sequence is homozygous; wherein the endogenous DNA sequence has a nucleic acid sequence identical to a sequence that A) exhibits a sequence comprising the coding sequence of SEQ ID NO: 4, B) comprises a nucleotide sequence exhibiting at least 98% sequence identity to the coding sequence of SEQ ID NO: 4; C) is complementary to SEQ ID NO: 4; or D) encodes a protein comprising an amino acid sequence exhibiting at least 98% sequence identity to SEQ ID NO: 6.

17. A Beta vulgaris plant, produced by the method of claim 16.

18. A Beta vulgaris plant, wherein bolting and flowering is inhibited after vernalization, wherein the plant comprises one or more mutations in an endogenous DNA sequence comprising a nucleic acid sequence identical to a sequence that a) comprises a sequence comprising the coding sequence of SEQ ID NO: 4, b) comprises a nucleotide sequence exhibiting at least 98% sequence identity to the coding sequence of SEQ ID NO: 4, c) is complementary to SEQ ID NO: 4, or d) encodes a protein comprising the amino acid sequence of SED ID NO: 6 or encodes a protein comprising an amino acid sequence exhibiting at least 98% sequence identity to SEQ ID NO: 6, wherein the one or more mutations cause a reduction of the activity or stability of the protein or polypeptide encoded by the endogenous DNA sequence compared to a non-mutagenized wild type plant.