U.S. patent application number 16/966853 was filed with the patent office on 2021-02-11 for fecal fungome and therapeutic efficacy of fecal microbiota transplantation.
The applicant listed for this patent is The Chinese University of Hong Kong. Invention is credited to Ka Leung Francis CHAN, Siew Chien NG, Tao ZUO.
Application Number | 20210038663 16/966853 |
Document ID | / |
Family ID | 1000005223560 |
Filed Date | 2021-02-11 |
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United States Patent
Application |
20210038663 |
Kind Code |
A1 |
NG; Siew Chien ; et
al. |
February 11, 2021 |
FECAL FUNGOME AND THERAPEUTIC EFFICACY OF FECAL MICROBIOTA
TRANSPLANTATION
Abstract
Methods are provided for identifying subjects as suitable donor
or recipients for FMT, for assessing the likelihood of FMT
treatment success, and for enhancing FMT treatment efficacy. Also
provided are kits and compositions for FMT with enhanced
efficacy.
Inventors: |
NG; Siew Chien; (Hong Kong,
CN) ; ZUO; Tao; (Qingzhou, CN) ; CHAN; Ka
Leung Francis; (Hong Kong, CN) |
|
Applicant: |
Name |
City |
State |
Country |
Type |
The Chinese University of Hong Kong |
Shatin, New Territories, Hong Kong SAR |
|
CN |
|
|
Family ID: |
1000005223560 |
Appl. No.: |
16/966853 |
Filed: |
February 1, 2019 |
PCT Filed: |
February 1, 2019 |
PCT NO: |
PCT/CN2019/074353 |
371 Date: |
July 31, 2020 |
Related U.S. Patent Documents
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Application
Number |
Filing Date |
Patent Number |
|
|
62625705 |
Feb 2, 2018 |
|
|
|
62679417 |
Jun 1, 2018 |
|
|
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Current U.S.
Class: |
1/1 |
Current CPC
Class: |
C12R 1/725 20130101;
A61P 1/12 20180101; C12N 1/20 20130101; A61K 36/064 20130101 |
International
Class: |
A61K 36/064 20060101
A61K036/064; A61P 1/12 20060101 A61P001/12; C12N 1/20 20060101
C12N001/20 |
Claims
1. A method for assessing likelihood of effective fecal microbiota
transplantation (FMT), comprising determining C. albicans level in
a stool sample obtained from a potential recipient prior to
FMT.
2. The method of claim 1, wherein the C. albicans level is a
percentage relative abundance.
3. The method of claim 2, wherein the C. albicans level is greater
than 10% and FMT is assessed as unlikely to be effective for the
potential recipient.
4. The method of claim 3, wherein the recipient is administered an
effective amount of an antifungal agent that suppresses C. albicans
growth before FMT.
5. The method of claim 4, further comprising determining C.
albicans level in a stool sample obtained from the recipient after
FMT.
6. The method of claim 2, wherein the C. albicans level is no
greater than 10% and FMT is assessed as likely to be effective for
the potential recipient.
7. The method of claim 1, further comprising determining total
fungal load in the stool sample.
8. The method of claim 7, further comprising performing FMT on the
potential recipient.
9. The method of claim 1, wherein C. albicans level is determined
in a first stool sample obtained from a first potential recipient
prior to FMT and in a second stool sample obtained from a second
potential recipient prior to FMT.
10. The method of claim 9, wherein the first potential recipient
has a lower C. albicans level than the second potential recipient
and is assessed to have a higher likelihood of effective FMT than
the potential recipient.
11. The method of claim 9, wherein the second potential recipient
is administered an effective amount of an antifungal agent that
suppresses C. albicans growth before FMT.
12. The method of claim 1, wherein the potential recipient has
inflammatory bowel disease (IBD) with concurrent Clostridium
difficile infection (CDI).
13. The method of claim 12, wherein the C. albicans level is
determined in stool samples taken from the recipient before and
after FMT.
14. A method for identifying a suitable donor for FMT, comprising
the step of determining C. albicans level in a stool sample
obtained from a candidate.
15. The method of claim 14, wherein the C. albicans level is a
percentage relative abundance.
16. The method of claim 15, wherein the C. albicans level is no
greater than 0.1% and the candidate is identified as a suitable
donor for FMT.
17. The method of claim 15, wherein the C. albicans level is
greater than 10% and the candidate is identified as an unsuitable
donor for FMT.
18. The method of claim 14, further comprising determining
Saccharomyces level and Aspergillus level in the stool sample.
19. The method of claim 14, further comprising determining
Escherichia level and Proteus level in the stool sample.
20. The method of claim 14, further comprising determining total
fungal load in the stool sample.
21. A method for improving FMT efficacy, comprising administering
to an FMT recipient prior to FMT an effective amount of an
antifungal agent that suppresses C. albicans growth.
22. The method of claim 21, wherein C. albicans level is determined
in a stool sample from the FMT recipient prior to administration of
the antifungal agent.
23. The method of claim 21, wherein C. albicans level is determined
in a stool sample from the FMT recipient after administration of
the antifungal agent.
24. The method of claim 21, further comprising administering to the
recipient prior to FMT an effective amount of an agent that reduces
total fungal load in a stool sample taken from the recipient prior
to FMT.
25. The method of claim 21, wherein the recipient has inflammatory
bowel disease (IBD) with concurrent Clostridium difficile infection
(CDI).
26. A kit comprising (1) a first composition comprising donor
stool; and (2) a second composition comprising an effective amount
of an antifungal agent that suppresses C. albicans growth.
27. The kit of claim 22, wherein the first composition comprises
donor stool that has been dried, frozen, and placed in a capsule
for oral ingestion.
28. The kit of claim 22, further comprising in the second
composition or in a third composition an effective amount of an
agent that reduces total fungal load.
29. (canceled)
Description
BACKGROUND OF THE INVENTION
[0001] This application claims priority to U.S. Provisional Patent
Application No. 62/625,705, filed Feb. 2, 2018, and U.S.
Provisional Patent Application No. 62/679,417, filed Jun. 1, 2018,
the contents of both are hereby incorporated by reference in the
entirety for all purposes.
BACKGROUND OF THE INVENTION
[0002] Clostridium difficile infection (CDI) is a symptomatic
infection due to the spore-forming bacterium, Clostridium
difficile. C. difficile infection is spread by bacterial spores
found within feces. Risk factors for infection include antibiotic
or proton pump inhibitors use, hospitalization, other health
problems, and older age. Its symptoms including watery diarrhea,
fever, nausea, and abdominal pain, CDI makes up about 20% of cases
of antibiotic-associated diarrhea. About 453,000 cases C. difficile
infection occurred in the United States in 2011, resulting in
29,000 deaths. Each year, C. difficile infections accounts for
health care cost of approximately $1.5 billion. Globally, rates of
C. difficile infection have increased between 2001 and 2016,
typically with more women than men affected by the infections.
[0003] Fecal microbiota transplantation (FMT) is highly effective
for the treatment of CDI, especially among patients suffering from
recurrent CDI. Also known as stool transplant, FMT involves a
process of transplanting fecal matter containing microorganism from
a healthy individual into the gastrointestinal tract of a
recipient. The goal of FMT is restoration of the gut microflora
disrupted due to CDI by introducing (or re-introducing) healthy
bacterial flora via various means of infusion of a healthy
individual's stool, e.g., by colonoscopy, enema, orogastric tube,
or by mouth in the form of a capsule containing freeze-dried
material obtained from a healthy donor. Aside from CDI, FMT is
increasingly being used to treat other intestinal and
extra-intestinal diseases, including other gastrointestinal
diseases, such as inflammatory bowel disease (IBD),
antibiotic-resistant bacterial infection, diarrhea, constipation,
irritable bowel syndrome, autism, depression, obesity, diabetes,
alopecia, and the like. In addition, FMT has been used for treating
certain neurological conditions, such as multiple sclerosis and
Parkinson's Disease. Considering the prevalence of CDI and other
conditions treatable by FMT in the human population and their
significant economic implications, there exists an urgent need for
developing new and improved methods for treating CDI and other
disorders by FMT with enhanced efficacy. The present invention
fulfills this and other related needs.
BRIEF SUMMARY OF THE INVENTION
[0004] The invention relates to novel methods and compositions
useful for more effectively treating Clostridium difficile
infection (CDI) and other diseases suitable by fecal microbiota
transplantation (FMT) treatment. In particular, the present
inventor discovered that, when elevated level of the yeast species
Candida albicans is present in the gastrointestinal tract of an FMT
recipient or in the stool of an FMT donor, therapeutic efficacy of
FMT is negatively impacted. This finding allows the inventors to
devise methods and compositions that can improve FMT effectiveness.
Thus, in the first aspect, the present invention provides a novel
method for assessing the likelihood of effective FMT. The method
includes a step of determining C. albicans level in a stool sample
obtained from a potential recipient prior to FMT is performed,
i.e., before the recipient is to receive transplantation of a donor
fecal material.
[0005] In some embodiments, the C. albicans level is a percentage
relative abundance, or is expressed as a percentage over the total
level of all fungal species in the sample. In some embodiments,
when the C. albicans level is determined as greater than 10% of
total fungi in the sample of the recipient, FMT is assessed as
unlikely to be effective for the potential recipient. Under such a
determination, the recipient in some cases will not receive FMT
treatment but will receive another different therapy; in other
cases, the recipient is administered an effective amount of an
antifungal agent that suppresses C. albicans growth before FMT is
performed. Optionally, after the administration of the antifungal
agent, the C. albicans level in the recipient (e.g., in a
recipient's stool sample) is again measured and determined to have
been lowered before the recipient is transplanted with a
composition containing donor fecal material. In some cases, C.
albicans level is again determined in a stool sample obtained from
the recipient after FMT.
[0006] In some embodiments, when the C. albicans level is no
greater than 10% of total fungi in the sample, FMT is assessed as
likely to be effective for the potential recipient. In some cases,
the potential recipient is then immediately given FMT, without any
further treatment or preparation such as administration of an
antifungal agent in the effective amount. In some embodiments, the
method further involves a step of determining total fungal load in
the stool sample. A potential recipient whose total fungal load in
his stool sample is found to be relatively lower than that of a
second potential recipient is expected to have a higher likelihood
of having a successful FMT than the second recipient. In some
embodiments, multiple potential recipients of FMT are tested prior
to FMT using this method for assessing their relative likelihood of
success upon receiving FMT treatment. For instance, C. albicans
level is determined in a first stool sample obtained from a first
potential recipient prior to FMT, and in the meantime C. albicans
level is determined in a second stool sample obtained from a second
potential recipient prior to FMT. In some embodiments, when the
first potential recipient has a lower C. albicans level than the
second potential recipient and is therefore assessed to have a
higher likelihood of effective FMT than the second potential
recipient. In some embodiments, the second potential recipient is
then administered an effective amount of an antifungal agent that
suppresses C. albicans growth before FMT, whereas the first
potential recipient may or may need antifungal agent treatment
prior to FMT. In some embodiments, C. albicans level is determined
by quantitative polymerase chain reaction (PCR). In some
embodiments, the levels of all fungal species present in the sample
is determined by the Internal transcribed spacer 2 (ITS2)
sequencing. In some embodiments, the recipient or recipients suffer
from inflammatory bowel disease (IBD) with concurrent Clostridium
difficile infection (CDI). The C. albicans level in the stool may
be determined before and after their FMT process. An elevated C.
albicans level before or after FMT is indicative of a higher
likelihood of poor outcome or ineffective FMT.
[0007] In a second aspect, the present invention provides a novel,
improved method for identifying a suitable donor who would provide
stool or fecal material for FMT. The method includes the step of
determining C. albicans level in a stool sample obtained from a
candidate who is being considered as a potential donor for FMT.
[0008] In some embodiments, the C. albicans level determined in
this method is a percentage relative abundance. In some cases, when
the C. albicans level is no greater than 0.1% of all fungi present
in the sample, the candidate is identified as a suitable donor for
FMT. Optionally, fecal matter such as stool is immediately
collected from the candidate for use in FMT. In some cases, when
the C. albicans level is greater than 0.1%, the candidate is deemed
unsuitable as a donor for FMT. As a result, either no fecal matter
is collected from the candidate at all; or fecal matter is
collected for processing to be used in FMT after the candidate is
given an effective amount of an antifungal agent that suppresses C.
albicans growth and again tested to find a satisfactorily reduced
C. albicans level in the stool sample (e.g., no greater than 0.1%
of total fungi in the sample). In some embodiments, the stool
sample of a candidate is tested for Saccharomyces level and
Aspergillus level in addition to C. albicans level. In some
embodiments, C. albicans level is determined by quantitative PCR.
In some embodiments, the levels of all fungi present in the sample
is determined by ITS2 sequencing. In some embodiments, the method
includes the additional step of determining Escherichia level and
Proteus level in the stool sample of a potential donor. Among
multiple potential donors, one with a relatively high Escherichia
level and a relatively low Proteus level is deemed a more suitable
donor than one with a relatively low Escherichia level and/or a
relatively high Proteus level. In some embodiments, the method
further includes a step of determining the total fungal load in the
stool sample taken from the potential donor. A potential donor
whose total fungal load in his stool sample is found to be
relatively lower than that of a second potential donor is expected
to be a more desirable donor, i.e., provide a higher likelihood of
a successful FMT, than the second donor.
[0009] In a third aspect, the present invention provides a method
for improving FMT efficacy. The method includes the step of
administering to an FMT recipient prior to FMT being performed an
effective amount of an antifungal agent that suppresses C. albicans
growth. In some embodiments of this method, C. albicans level is
first determined in a stool sample from the FMT recipient prior to
administration of the antifungal agent. In some embodiments, C.
albicans level is determined in a stool sample from the FMT
recipient after administration of the antifungal agent. In some
embodiments, C. albicans level is determined by quantitative PCR.
In some embodiments, the levels of all fungi present in the sample
is determined by ITS2 sequencing. In some embodiments, the method
further includes a step of administering to the recipient prior to
FMT an effective amount of an agent (e.g., an anti-fungal agent,
such as a broad-spectrum fungicide), which reduces total fungal
load in a stool sample taken from the recipient prior to FMT. In
some embodiments, the recipient is a patient suffering from
inflammatory bowel disease (IBD) with concurrent Clostridium
difficile infection (CDI).
[0010] In a fourth aspect, the present invention provides kits and
compositions useful for enhanced FMT treatment with improved
efficacy. In some embodiments, a kit for improving FMT efficacy
includes a first composition comprising a donor stool material and
a second composition comprising an effective amount of an
antifungal agent capable of suppressing the growth of C. albicans.
Typically, the first and second compositions are kept in two
separate containers. In some embodiments, the first composition
contains processed donor fetal matter and is formulated for FMT by
direct transfer to the GI tract (e.g., via colonoscopy or via nasal
intubation) or by oral ingestion. In some embodiments, the first
composition comprises donor fecal matter further fortified with an
additional and effective amount of one or more fungal species
belonging to the genus Saccharomyces and/or the genus Aspergillus.
In some embodiments, the second composition is formulated for
administration of the antifungal agent (such as fluconazole) to the
recipient by injection, oral ingestion, or rectal deposit. In some
embodiments, the kit may further comprise, either in the second
composition or in a third composition, an effective amount of an
agent that reduces total fungal load. In the alternative, the kit
may comprise a third composition, which comprises an effective
amount of an agent that reduces total fungal load, but not the
second composition. In some cases, the kit may further include
printed user instructions.
[0011] Related compositions useful in FMT with improved efficacy
may comprise (1) a donor stool material containing live fecal
microorganisms and (2) an antifungal agent that specifically
suppresses the growth or proliferation of C. albicans but exhibits
no such suppressive or inhibitory effect against other fungal
species. Instead of a broad-spectrum fungicide, such specific
anti-C. albicans agent may be short polynucleotide in nature of
(e.g., a small inhibitory RNA, microRNA, miniRNA, lncRNA, or an
antisense oligonucleotide that is capable of disrupting the
expression of a key gene in the life cycle of C. albicans) that is
capable of specifically targeting the species only but not other
closely related fungal species.
BRIEF DESCRIPTION OF THE DRAWINGS
[0012] FIG. 1 Fungal alterations in CDI. (A) Comparison of the
fecal mycobiota based on Shanon diversity, evenness, Chao1 richness
in controls and CDI subjects. The bars are shown in median and
interquartile range. The dots indicate individual values of the
studied subjects. Statistical significance was determined by
Mann-Whitney test, *P<0.05, **P<0.01. (B) Fungal community
structure difference between controls and CDI by NMDS (Non-metric
multidimensional scaling) plot based upon Bray-Curtis distance
among all samples. (C) Comparison of the fecal mycobiota
composition between controls and CDI subjects at the phylum level.
(D) Differentially enriched fungal species between controls and
CDI. Statistical significance was determined by LefSe analysis with
FDR correction (only those species with q values<0.05 and LDA
effect size>2 are shown). Heatmap of the presence of these
differential fungal species is shown in relative abundance
intensity. LDA effect size, q value (FDR-adjusted P value) and
species annotation are shown. Green bars and dots indicate species
enriched in controls, while red bar and dot indicate species
enriched in CDI. (E) Comparison of the relative abundance of fecal
C. albicans in controls and CDI subjects. The bars are shown in
median and interquartile range. Statistical significance was
determined by Mann-Whitney test, *P<0.05.
[0013] FIG. 2 Colonization of donor-derived fungal and bacterial
taxa in FMT recipients in association with treatment response. (A)
Presence of fungal operational taxonomic units (OTUs) in FMT
recipients at the last follow-up. The color of the bar indicates
the origin of the bacterial OTUs in the recipient. Purple indicates
donor-derived OTUs colonized in the recipient, orange indicates
OTUs exclusively present in recipient at baseline but not in donor
at baseline, while green indicates OTUs present both in donor and
in recipient before FMT. Comparison of the frequency of donor
derived bacterial OTUs in FMT responders and in non-responders is
shown. Statistical significance was determined by Mann-Whitney
test, **P<0.01. (B) Presence of bacterial OTUs in FMT recipients
at the last follow-up. Comparison of the frequency of donor-derived
bacterial OTUs in FMT responders and in non-responders is shown.
Statistical significance was determined by Mann-Whitney test,
*P<0.05. (C) Heatmap of the abundance of differentially
presented fungal genera in donor, pre-FMT and post-FMT last
follow-up samples. Fungal genera with disparate presence between
FMT responders and non-responders, as determined by LefSe analysis,
are labeled with asterisk (genera with LDA effect size>2 and q
value<0.01).
[0014] FIG. 3 Post-FMT alterations in the enteric mycobiota of CDI
recipients in association with FMT response. Fecal fungal richness
(A) and diversity (B) alterations in FMT recipients over the course
of longitudinal follow-up and in their corresponding donors at
baseline. Comparison of the fungal richness and diversity of
pre-FMT samples and post-FMT samples collected at the last
follow-up are shown in FMT responders and FMT non-responders
respectively. Statistical significance was determined by paired
Wilcox signed rank test, *P<0.05. "F" indicates FMT treated
subject. "W" indicates weeks post treatment. (C) Alterations in the
fecal fungal composition at the genus level in CDI recipients after
FMT at different time points up to the last follow-up. (D)
Differentially enriched fungal taxa across post-FMT fecal samples
of FMT responders versus non-responders at the genus and species
level respectively. Statistical significance level was determined
by LefSe analysis with FDR correction (only those taxa with q
values<0.05 and LDA effect size>2 are shown). Green bars and
dots indicate taxa enriched in controls, while red bar and dot
indicate taxa enriched in CDI. (E) Alterations of the relative
abundance of fecal C. albicans after FMT at the last follow-up in
FMT recipients. Statistical significance was determined by paired
Wilcox signed rank test, *P<0.05. (F) Relative abundance of C.
albicans in donor stool corresponding to FMT responders and
non-responders. Statistical significance was determined by
Chi-square test. (G) Relative abundance of C. albicans in stool of
recipients before FMT in association with FMT response. Statistical
significance was determined by Chi-square test.
[0015] FIG. 4 C. albicans compromises FMT efficacy in eradicating
C. difficile infection in mice. A) Schematic diagram of C. albicans
administration and stool infusion (FMT) in a murine C. difficile
infection (CDI) model. Antibiotic treatment was ceased before
gavage of C. albicans (CA) and C. difficile. B) Diarrhoea in mice
on day 1 after stool infusion. C) Representative H&E-stained
colonic sections on day 2 after stool infusion (shaded star denotes
inflammatory cells infiltration, hallowed star denote ulceration,
asterisk denotes oedema, arrow denotes goblet cell loss and
triangle denotes herniated crypts). Scale bar, 150 .mu.m. n=5 mice
per group. D) Enumeration of C. difficile in feces of mice on day 0
before FMT and day 1 post FMT (n=9 mice per group). Statistical
significance represents comparisons between FMT-treated mice with
C. difficile infection versus other groups by unpaired Mann-Whitney
test. *P<0.05, **P<0.01. E) Enumeration of C. albicans in
feces of mice both on day 0 before FMT and day 1 post FMT (n=9 mice
per group). Statistical significance represents comparison between
C. albicans load on day 0 before FMT and day 1 post FMT, by paired
Mann-Whitney test. *P<0.05. Dot graphs show means.+-.s.e.m,
performed at least two times independently.
[0016] FIG. 5 Quantification of fecal C. albicans levels in CDI
subjects and healthy controls by qPCR. a, qPCR detection of C.
albicans on CDI subjects and healthy controls from the discovery
cohort. Comparison of the fecal C. albicans level between control
and CDI was determined by Mann-Whitney test, ****P<0.0001. b,
qPCR detection of C. albicans on an additional set of subjects (17
healthy individuals, 12 CDI subjects with and 12 without antibiotic
use at inclusion). Statistical significance was tested by unpaired
Mann-Whitney test. *P<0.05, **P<0.01. Graphs are shown in
mean.+-.s.e.m. ND denotes no detectable C. albicans in the feces as
determined by quantitative PCR.
[0017] FIG. 6 Longitudinal timeline of stool sample collection
(expressed in weeks). "F" indicates FMT treated subject. "Donor"
indicates FMT donor. "S" indicates subject treated with standard
therapy (STD, vancomycin). "W" indicates weeks post treatment. Red
dots indicate donor samples, green dots indicate FMT recipient
samples sampled at different time points.
[0018] FIG. 7 Heatmap of the abundance of differentially presented
bacterial genera in donor, pre-FMT and post-FMT last follow-up
samples.
[0019] FIG. 8 Post-FMT alterations in the enteric bacterial
microbiota of CDI recipients in association with FMT response. (A)
Comparison of the fecal bacterial shanon diversity, evenness, chao1
richness in healthy controls and in CDI subjects. The bars are
shown in median and interquartile range. The dots indicate
individual values of the studied subjects. Statistical significance
was determined by Mann-Whitney test, **P<0.01. Fecal bacterial
richness (B) and diversity (C) alterations in FMT recipients over
the course of longitudinal follow-up and in their corresponding
donors at baseline. Comparison of the fungal richness and diversity
of pre-FMT samples and post-FMT samples collected at the last
follow-up are shown in FMT responders and FMT non-responders
respectively. Statistical significance was determined by paired
Wilcox signed rank test, *P<0.05. "F" indicates FMT treated
subject. "W" indicates weeks post treatment.
[0020] FIG. 9 Post-antibiotic alterations in the enteric mycobiota
of CDI subjects treated with vancomycin in association with
treatment response. Fecal fungal richness (A) and diversity (B)
alterations over the course of longitudinal follow-up in CDI
subjects who received vancomycin treatment. "S" indicates CDI
subject received vancomycin treatment (standerd therapy, STD). "W"
indicates weeks post vancomycine treatment. (C) Frequencies of CDI
individuals increased or decreased in fungal diversity and richness
post treatment with respect to FMT and STD treatment. Statistical
significance was determined by Chi-square test, *P<0.05. (D)
Comparison of post-FMT fold change (FC) of the fecal fungal
diversity relative to the pre-FMT sample in FMT responders and STD
responders. Statistical significance was determined by Chi-square
test, *P<0.05. (E) Comparison of post-FMT fold change (FC) of
the fecal fungal richness relative to the pre-FMT sample in FMT
responders and STD responders. Statistical significance was
determined by Man-whitney test, *P<0.05. (G) Alterations in the
fecal fungal composition at the genus level in CDI subjects on
vancomycin regime at different time points up to the last
follow-up.
[0021] FIG. 10 Fecal bacterial microbiota richness (A) and
diversity (B) alterations in STD subjects over the course of
longitudinal follow-up. "S" indicates vancomycin treated subject
(STD treatment). "W" indicates weeks post treatment.
[0022] FIG. 11 Differentially enriched fungal taxa across
post-treatment samples of FMT responders versus STD responders at
the family, genus and species levels. Statistical significance
level was determined by lefSe analysis with FDR correction (only
those taxa with q values<0.05 and LDA effect size>2 are
shown).
[0023] FIG. 12 Post-STD alterations of the relative abundance of
fecal C. albicans at the last follow-up and at baseline in CDI
subjects on vancomycin treatment. Statistical significance was
determined by paired Wilcox signed rank test.
[0024] FIG. 13 Spearman correlation between fungal diversity,
evenness, richness and bacterial diversity, evenness, richness,
with respect to FMT and STD treatment and treatment response.
Statistical significance was determined for all pairwise
comparisons; significant correlations (P value<0.05) are
displayed with asterisk. Blue circles and positive values indicate
positive correlations, red circles and negative values indicate
inverse correlations. The size and shading indicate the magnitude
of the correlation where darker shades are more intensively
correlated than lighter ones.
[0025] FIG. 14 Trans-kingdom interactions between bacteria and
fungi. Spearman correlation plots of the relative abundance of
fungal genera and bacterial genera identified to be significantly
associated with CDI and controls at baseline, with respect to FMT
and STD treatment and treatment response. Spearman correlation
coefficients were calculated for all pairwise comparisons; Blue
circles and positive values indicate positive correlations, red
circles and negative values indicate inverse correlations. The size
and shading indicate the magnitude of the correlation where darker
shades are more intensively correlated than lighter ones.
Statistical significance was determined for all pairwise
comparisons; only correlations tested significant (P value<0.05)
are displayed.
[0026] FIG. 15 Pre-FMT eradication of C. albicans in recipient mice
restored FMT efficacy in clearing C. difficile infection. A)
Schematic diagram of antifungal treatment and stool infusion (FMT)
in a murine model with di-colonisation of C. albicans and C.
difficile. Antifungal (fluconazole) treatment was ceased at day 4
after administration of C. albicans when C. albicans was determined
negative by cultivation. "CCfF", mouse group with di-colonisation
of C. albicans and C. difficile and treatments of fluconazole and
FMT; "CCF", mouse group with di-colonisation of C. albicans and C.
difficile and treatment of FMT; "CC", mouse group with
di-colonisation of C. albicans and C. difficile. B) Enumeration of
C. difficile in feces of mice on day 0 before FMT and day 1 post
FMT (n=10 mice per group). Statistical significance was determined
by unpaired Mann-Whitney test. *P<0.05, **P<0.01. C)
Enumeration of C. albicans in feces of mice both on day 0 before
FMT and day 1 post FMT (n=10 mice per group). Statistical
significance represents comparison between C. albicans load on day
0 before FMT and day 1 post FMT, by paired Mann-Whitney test.
**P<0.01. Dot graphs show means.+-.s.e.m, performed at least two
times independently.
[0027] FIG. 16 The presence of C. albicans is linked to FMT
outcomes in CDI. The absolute abundance of fecal C. albicans before
and after FMT at the last follow-up in FMT recipients, assessed by
quantitative PCR. Comparison of the fecal C. albicans levels
between pre-FMT samples and post-FMT samples was performed by
paired Wilcoxon signed rank test, *P<0.05. Comparison of the
fecal C. albicans levels between the post-FMT samples of FMT
responders and FMT non-responders was performed by Mann-Whitney
test, .sup.$$P<0.01. ND denotes no detectable C. albicans in the
feces.
[0028] FIG. 17 Comparison of the total fungal load in the feces of
controls and CDI subjects. Statistical significance was determined
by Mann-Whitney test, ***P<0.001.
[0029] FIG. 18 The total fecal fungal load and C. albicans in
inflammatory bowel disease (IBD). a. Comparison of the total fungal
load in the feces of controls and IBD subjects, including patients
with CD and UC. Statistical significance was determined by
Mann-Whitney test, ***P<0.001, *P<0.05. b. Comparison of C.
albicans levels in the feces of controls and CDI subjects.
[0030] FIG. 19 The total fecal fungal load in irritable bowel
syndrome (IBS). Comparison of the total fungal load in the feces of
controls and IBS subjects. Statistical significance was determined
by Mann-Whitney test, ***P<0.001, **P<0.05.
[0031] FIG. 20 Post-FMT alterations in the taxonomic composition of
the bacterial microbiota of CDI recipients in association with FMT
response. Bacterial configurations in FMT recipients over the
course of longitudinal follow-up and in their corresponding donors
at baseline, at the phylum (a) and family (b) levels. c, Heatmap of
the relative abundance of differentially presented bacterial genera
in donor, pre-FMT and post-FMT last follow-up samples. d,
Differentially presented bacteria taxa across post-FMT samples of
FMT responders versus non-responders at the phylum, family, and
genus levels. Statistical significance level was determined by
LefSe analysis with FDR correction (only those taxa with q
values<0.05 and LDA effect size>2 are shown).
[0032] FIG. 21 Quantification of fecal C. albicans levels in IBD
concurrent with CDI subjects before and after FMT. The fecal C.
albicans level (gDNA content/fecal DNA) was determined by qPCR.
PRB, peri rectal bleeding.
[0033] Definitions
[0034] The term "fecal microbiota transplantation (FMT)" or "stool
transplant" refers to a medical procedure during which fecal matter
containing live fecal microorganisms (bacteria, fungi, and he like)
obtained from a healthy individual is transferred into the
gastrointestinal tract of a recipient to restore healthy gut
microflora that has been disrupted or destroyed by a variety of
medical conditions. Typically, the fecal matter from a healthy
donor is first processed into an appropriate form for the
transplantation, which can be made through direct deposit into the
lower gastrointestinal tract such as by colonoscopy, or by nasal
intubation, or through oral ingestion of an encapsulated material
containing dried and frozen fecal matter. Clostridium difficile
infection (CDI) is the condition most commonly treated by FMT,
although a number of other diseases and disorders including in the
digestive system and in the nervous system have been reported to be
successfully treated by FMT.
[0035] The term "inhibiting" or "inhibition," as used herein,
refers to any detectable negative effect on a target biological
process, such as RNA/protein expression of a target gene, the
biological activity of a target protein, cellular signal
transduction, cell proliferation, and the like. Typically, an
inhibition is reflected in a decrease of at least 10%, 20%, 30%,
40%, 50%, 60%, 70%, 80%, 90% or greater in the target process
(e.g., growth or proliferation of fungal cells), or any one of the
downstream parameters mentioned above, when compared to a control.
"Inhibition" further includes a 100% reduction, i.e., a complete
elimination, prevention, or abolition of a target biological
process or signal. The other relative terms such as "suppressing,"
"suppression," "reducing," and "reduction" are used in a similar
fashion in this disclosure to refer to decreases to different
levels (e.g., at least 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%
or greater decrease compared to a control level) up to complete
elimination of a target biological process or signal. On the other
hand, terms such as "activate," "activating," "activation,"
"increase," "increasing," "promote," "promoting," "enhance,"
"enhancing," or "enhancement" are used in this disclosure to
encompass positive changes at different levels (e.g., at least 10%,
20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, 100%, 200%, or greater such
as 3, 5, 8, 10, 20-fold increase compared to a control level) in a
target process or signal.
[0036] As used herein, "C. albicans" refers to a fungal species
belonging to the Candida genus, Saccharomycetaceae family,
Saccharomycetales order, Saccharomycetes class, and Ascomycota
division. A common member of the human gut flora, C. albicans is a
potential yeast pathogen capable of causing opportunistic infection
in humans, especially in those with compromised immune system.
[0037] The term "antifungal agent" refers to any substance that is
capable of inhibiting, suppressing, or preventing the growth or
proliferation of fungal species, especially those of the Ascomycota
division, such as C. albicans. Known agents with fungicidal
activity include amphotericin B, echinocandin, fluconazole,
nystatin, and clotrimazole.
[0038] "Percentage relative abundance," when used in the context of
describing the presence of a particular fungal species (e.g., C.
albicans) in relation to all fungal species present in the same
environment, refers to the relative amount of the fungal species
out of the amount of all fungal species as expressed in a
percentage form. For instance, the percentage relative abundance of
C. albicans can be determined by comparing the quantity of C.
albicans-specific DNA (e.g., determined by quantitative polymerase
chain reaction) in one given sample with the quantity of all fungal
DNA (e.g., determined by quantitative PCR and sequencing based on
the Internal transcribed spacer 2 or ITS2 sequence) in the same
sample.
[0039] "Absolute abundance," when used in the context of describing
the presence of a particular fungal species (e.g., C. albicans) in
the feces, refers to the amount of DNA derived from the fungal
species out of the amount of all DNA in a fecal sample. For
instance, the absolute abundance of C. albicans can be determined
by comparing the quantity of C. albicans-specific DNA (e.g.,
determined by quantitative polymerase chain reaction) in one given
sample with the quantity of all fecal DNA in the same sample.
[0040] "Total fungal load" of a fecal sample, as used herein,
refers to the amount of all fungal DNA out of the amount of all DNA
in the fecal sample. For instance, the absolute abundance of fungi
can be determined by comparing the quantity of fungal specific DNA
(e.g., 18S rDNA determined by quantitative polymerase chain
reaction) in one given sample with the quantity of all fecal DNA in
the same sample.
[0041] The term "effective amount," as used herein, refers to an
amount of a substance that produces a desired effect (e.g., an
inhibitory or suppressive effect on C. albicans growth) for which
the substance (e.g., an antifungal agent) is used or administered.
The effects include the prevention, inhibition, or delaying of any
pertinent biological process during C. albicans growth or
development to any detectable extent. The exact amount will depend
on the nature of the substance (the active agent), the manner of
use/administration, and the purpose of the application, and will be
ascertainable by one skilled in the art using known techniques as
well as those described herein.
[0042] As used herein, the term "about" denotes a range of value
that is +/-10% of a specified value. For instance, "about 10"
denotes the value range of 10+/-10.times.10%, i.e., 9 to 11.
DETAILED DESCRIPTION OF THE INVENTION
I. Introduction
[0043] The invention provides a novel approach for assessing the
likelihood of effective FMT prior to the procedure being performed
as well as for improving the effectiveness of the FMT procedure.
During their studies, the present inventors discovered that the
presence and relative abundance of certain fungal species both in a
recipient's gastrointestinal tract and in a donor's stool directly
correlate with the outcome of FMT. In particular, the fungal
species Candida albicans of the Saccharomycetaceae family is found
to negatively impact the effectiveness of FMT. The detection of C.
albicans in a potential donor's stool thus can be used to guide
donor selection, whereas analysis of C. albicans level in an FMT
recipient can determine whether the subject is immediately ready
for FMT or should be treated with an antifungal agent that
suppresses C. albicans growth prior to FMT in order to optimize the
therapeutic outcome.
II. FMT Donors and Recipients
[0044] Patients suffering from CDI, especially recurring CDI, are
often considered as recipients for FMT treatment. Aside from CDI,
other diseases and conditions, including those of digestive system
or nervous system such as colitis, irritable bowel syndrome,
multiple sclerosis, Parkinson's Disease, diabetes mellitus, and
obesity are also beginning to be considered for FMT treatment.
[0045] Fecal matter used in FMT is obtained from a healthy donor
and then processed into appropriate forms for the intended means of
delivery in the upcoming FMT procedure. Up until now, the general
criterion for an FMT donor is simply that the donor is a healthy
individual without any known diseases or disorders especially in
the digestive tract, although some preference is often given to the
members of the same household as the recipient.
[0046] The present inventors have discovered in their studies that
elevated presence of one particular fungal species, C. albicans, in
a recipient's gastrointestinal tract or in a donor stool (which is
used in the transplantation after being processed) can
significantly reduce efficacy of FMT treatment in a patient. In
contrast, successful FMT has been observed as correlating with
elevated presence of other fungal species, such as those belonging
to the genus of Saccharomyces or Aspergillus, in a recipient or in
a donor stool. This revelation enables the initial screening of
individuals as appropriate FMT donors as well as the initial
screening of patients as likely candidates for successful FMT
treatment: if a candidate donor's stool contains an elevated level
of C. albicans (e.g., greater than 0.1% of total fungi), the
candidate is deemed as unsuitable as an FMT donor, and his stool
should not be taken or used in FMT; if a candidate's stool sample
shows no or only low level of C. albicans (e.g., no greater than
0.1% of total fungi), then the candidate is deemed an appropriate
FMT donor and his fecal material can be immediately retrieved for
processing and later used in FMT. On the other hand, if a patient
who has been proposed to receive FMT treatment, and his stool
sample shows an elevated level of C. albicans (e.g., greater than
10% of total fungi), then the patient is deemed to be unsuitable to
receive FMT and is therefore not to be given FMT, as the therapy is
likely to be unsuccessful; if a patient's stool sample shows no or
only a low level of C. albicans (e.g., no greater than 10% of total
fungi), the patient is deemed an appropriate recipient for FMT who
is likely to enjoy therapeutic success from FMT, and thus can start
FMT treatment immediately without other steps of preparation or
pre-treatment.
[0047] Various methods have been reported in the literature for
determining the levels of all fungal species in a sample, for
example, amplification (e.g., by PCR) and sequencing of fungal
polynucleotide sequence by using the Internal transcribed spacer 2
(ITS2) sequence. On the other hand, the level of any given fungal
species may be determined by amplification and sequencing of its
signature 18S rRNA sequence. A percentage abundance is often used
as a parameter to indicate the relative level of a fungal species
in a given environment.
III. Methods for Improving FMT Efficacy
[0048] The discovery by the present inventors revealing the direct
correlation between an elevated level of C. albicans in FMT donor
or recipient and reduced efficacy of FMT treatment not only allows
one to devise an initial screening process to identify appropriate
donors and recipients for the FMT procedure, it also enables
different methods for improving FMT efficacy by reducing the level
of C. albicans in a donor and in a recipient prior to the FMT
treatment.
[0049] As discussed in the above section, when a candidate donor's
stool is tested and found to contain an elevated level of C.
albicans (e.g., greater than 0.1% of total fungi), the candidate is
deemed as unsuitable as an FMT donor, and his stool should not be
taken for use in FMT as it is unlikely to result in a successful
FMT treatment if used. Similarly, when a patient or proposed FMT
recipient whose stool is tested and found to contain an elevated
level of C. albicans (e.g., greater than 10% of total fungi), the
patient is deemed as an unsuitable recipient for FMT, and he should
not immediately undergo FMT due to the high probability of an
ineffective outcome. Yet these cases of expected unsuccessful
treatment outcome can be readily improved in view of the inventors'
discovery.
[0050] First, for a patient who has been considered for receiving
FMT but who has also been deemed an unsuitable recipient of FMT due
to an elevated level of C. albicans (e.g., above 10% of total
fungi) found in his/her stool sample, which indicates a diminished
chance of a successful FMT, measures can be taken to lower his/her
level of C. albicans before FMT is commenced so that a much greater
efficacy can be achieved for the FMT procedure. For instance, an
antifungal agent capable of suppressing the growth or proliferation
of C. albicans can be administered to the patient in an effective
amount such that the level of C. albicans in the patient's
digestive track and in the feces is significantly reduced (e.g., no
more than 10% of total fungi) prior to the start of the FMT
procedure. In this case, the patient's C. albicans level is to be
determined twice: once at the initial screening stage, a second
time after the initial level is deemed too high for an effective
FMT and after an antifungal agent has been given to the patient.
Once the C. albicans level is confirmed as lowered to a percentage
that would allow satisfactory FMT outcome, the patient is then
ready to undergo FMT treatment.
[0051] Second, for a candidate who has been deemed improper to
serve as an FMT donor due to a higher level of C. albicans in his
stool, the expected undesirable FMT outcome can be remedied by
treating the candidate donor with an effective amount of an
antifungal agent capable of suppressing the growth or proliferation
of C. albicans can be administered. Since the donor's body,
especially the gastrointestinal track, contains a vast collection
of microorganisms many of which are important for the health of gut
microflora and for the success of FMT, a useful antifungal agent
for this purpose cannot be a broad-spectrum fungicide. Rather, it
should be an agent that narrowly and precisely targets the species
of C. albicans without significantly affecting other fungal
species, including those closely related to C. albicans. Although
the agent may be of any chemical compound in nature, small
polynucleotides (e.g., siRNAs, miRNAs, miniRNAs, lncRNAs, or
antisense DNAs/RNAs) may be the most effective in achieving the
specific task of disrupting the expression of one or more key genes
in the life cycle of C. albicans so as to specifically inhibit the
proliferation of the target species only without significant impact
on other closely related fungal species.
[0052] Immediately upon completion of FMT procedure, the recipient
may be further monitored by continuous testing of the level of C.
albicans in the stool samples on a daily basis for up to 5 days
post-FMT while the clinical symptoms of the condition being treated
are also being monitored in order to assess FMT outcome and the
corresponding C. albicans level in the recipient.
IV. Kits and Compositions for Improved FMT
[0053] The present invention also provides novel kits and
compositions that can be used for improving FMT efficacy. For
example, in a kit for treating a patient in need of FMT, a first
composition intended for transplantation into a patient or FMT
recipient and a second composition intended to be administered to
the recipient for reducing the level of C. albicans in the
recipient. The first composition comprises a fecal material from a
donor, which has been processed, formulated, and packaged to be in
an appropriate form in accordance with the delivery means in the
FMT procedure, which may be by direct deposit in the recipient's
lower gastrointestinal track (e.g., wet or semi-wet form) or by
oral ingestion (e.g., frozen dried encapsulated). The second
composition comprises an antifungal agent capable of suppressing
the growth/proliferation of C. albicans, which may be a
broad-spectrum fungicide or a specific inhibitor of the C. albicans
species, and one or more pharmaceutically acceptable excipient. The
composition is formulated for the intended delivery method of the
antifungal agent, for example, by injection (intravenous,
intraperitoneal, intramuscular, or subcutaneous injection) or by
oral ingestion or by local deposit (e.g., suppositories). The first
and second compositions are often kept separately in two different
containers in the kit. Typically, the kit will further include
printed material providing detailed instructions for users of the
kit, such as providing information of the schedule and dosing
arrangement for administering the first and second compositions to
a recipient.
[0054] In another aspect of this invention, alternative
compositions useful in FMT with improved efficacy may be devised to
contain at least these two components: (1) a donor stool material
containing live fecal microorganisms, and (2) an antifungal agent
that specifically suppresses the growth or proliferation of C.
albicans but exhibits no such suppressive or inhibitory effect
against other fungal species. Component (2) preferably is not a
broad-spectrum fungicide; rather, it should be a specific anti-C.
albicans agent. For example, it may be short polynucleotide in
nature of, e.g., a small inhibitory RNA, microRNA, miniRNA, lncRNA,
or an antisense oligonucleotide, that is capable of disrupting the
expression of at least one key gene in the life cycle of C.
albicans, such that the agent is capable of specifically targeting
the species only without significantly affecting other closely
related fungal species. Component (2) is particularly useful in the
case of a donor's stool containing a level of C. albicans too high
to permit a satisfactory FMT outcome, as it is capable of locally
and specifically suppressing the proliferation of C. albincans so
as to ensure the success of FMT despite the less than desirable
quality of the donor fecal material.
EXAMPLES
[0055] The following examples are provided by way of illustration
only and not by way of limitation. Those of skill in the art will
readily recognize a variety of non-critical parameters that could
be changed or modified to yield essentially the same or similar
results.
Example 1
Introduction
[0056] Fecal microbiota transplantation (FMT) is effective in
treating recurrent Clostridium difficile infection (CDI) and is
increasingly being utilized in other human diseases. Whilst
bacteria colonization in recipients after FMT has been established,
little is known of the role of the gut mycobiota. In this study the
present inventors show gut fungal dysbiosis in CDI and identify
that donor-derived fungi colonization in recipient is associated
with FMT response. Mycobiota profiling in CDI reveals
over-presentation of C. albicans and decreased fungal diversity,
richness and evenness compared with healthy controls. Cure after
FMT was observed when donor-derived fungal taxa predominated in
recipients' mycobiota. FMT responders display a high prevalence of
Saccharomyces and Aspergillus whilst non-responders and individuals
treated with antibiotics display a dominant presence of Candida.
High abundance of C. albicans in recipient before FMT and in donor
stool nullifies FMT efficacy in eradicating CDI. Furthermore, C.
albicans compromises FMT efficacy in a mouse model of CDI, while
anti-fungal treatment reestablishes its efficacy. This study
furthers the knowledge of human gut mycobiota dynamics and their
contribution to FMT, and it enables an understanding of
personalized donor-recipient selection in future FMT studies for
various human diseases.
[0057] The past decade has witnessed an increasing use of fecal
microbiota transplantation (FMT) as a promising treatment option
for several diseases .sup.1-3, yet success rates are variable with
a cure rate of 85-90% in recurrent Clostridium difficile infections
(CDI).sup.3-5 and a response rate of 30-40% in inflammatory bowel
disease.sup.6-8. Such variations may be related to disease traits,
recipient factors or donor characteristics. The mechanisms
underlying a successful FMT and its relationship with gut microbial
profiles in donor-recipient pairs remain elusive. To date, the
efficacy of FMT has been mostly ascribed to the restoration of the
bacterial microbiota and a sustained co-existence of donor and
recipient bacterial strains.sup.9-12. Recently, bacteriophages have
been shown to be altered in CDI after FMT and these changes were
associated with treatment outcome .sup.13-15. The human
gastrointestinal tract is also colonized by a large population of
fungi, collectively referred to as the mycobiota, which play an
important role in human health.sup.16,17. Gut mycobiota contribute
to normal human physiology and in some cases can recapitulate the
benefit of intestinal bacteria via regulating host immunity and
maintaining intestinal homeostasis.sup.16,17,18. Whether
donor-derived mycobiota can colonize a recipient host, the fate of
donor and recipient mycobiota after FMT and their relationship with
treatment outcomes are unknown. The inventors performed internal
transcribed spacer 2 (ITS2) and 16S rDNA sequencing in FMT-treated
subjects with CDI to explore the effects of FMT on the gut
mycobiome in association with treatment outcome. A
proof-of-causality study was conducted in C. difficile-infected
mice to confirm the role of gut mycobiota in FMT response.
Results
Gut Fungal Dysbiosis in CDI
[0058] The fecal mycobiomes were compared between 31 CDI subjects
and 23 healthy controls. There was a significant decrease in fungal
diversity, evenness and richness in CDI compared with controls
(Mann-Whitney test, p=0.0120, 0.0309, and 0.0043, respectively,
FIG. 1a). The fungal communities of CDI subjects were significantly
separated from those of healthy controls at the OTU level (based on
Bray-Curtis distance, adonis test p=0.003, FIG. 1b). At the phylum
level, Ascomycota was expanded in CDI compared with controls
(Mann-Whitney test, p=0.0083, FIG. 1c). At the species level, 17
fungal species were found to be differentially present between CDI
and controls (LefSe analysis with FDR adjusted q<0.05, FIG. 1d).
Amongst these species, only C. albicans was significantly enriched
in CDI (FIG. 1d, e, Mann-Whitney test, p=0.0080), whereas 16 other
species were enriched in controls. In line with the observation at
the species level, more taxa were enriched in controls than in CDI,
as determined by LefSe analysis (9 versus 1 at the order level, 15
versus 2 at the family level, 16 versus 1 at the genus level, Table
2). Altogether, these data indicate dysbiosis of the enteric
mycobiota in patients with CDI.
[0059] Over-presentation of C. albicans in absolute abundance in
CDI was confirmed through quantitative PCR (FIG. 5a). Antibiotic
use has been shown to be a major contributor to the development of
CDI by decreasing bacterial colonization resistance. The effect of
antibiotics on C. albicans levels in CDI was further assessed.
Stool samples were collected from new consecutive CDI patients,
including 12 CDI patients with antibiotics exposure, 12 CDI
patients with no antibiotics exposure at inclusion, and 17 healthy
controls. Significantly higher levels of fecal C. albicans were
found in CDI subjects exposed to antibiotics at inclusion, compared
with controls (FIG. 5b, Mann-Whitney test, p=0.0131). C. albicans
levels were also significantly higher in CDI subjects not exposed
to antibiotics at inclusion when compared with controls (FIG. 5b,
Mann-Whitney test, p=0.0469). These data indicate that both CDI and
antibiotics are contributors to increased levels of C.
albicans.
Donor fungi colonization in recipient is associated with FMT
response
[0060] It was then explored whether FMT leads to colonization of
donor-derived fungi and its association with treatment efficacy.
Changes in the gut mycobiomes of recipients after FMT were
monitored at multiple time points in 16 CDI subjects, using pre-FMT
samples of each donor-recipient pair as a baseline for FMT (FIG.
6). Amongst 16 CDI subjects treated with FMT, nine remained
symptom-free with a negative stool C. difficile toxin at the last
follow-up (termed responders, FMT1-FMT9), whilst seven developed
recurrence of CDI (termed non-responders, FMT10-FMT16) (Table 1).
It was next investigated whether donor-derived fungi and bacteria
in recipients may influence FMT outcomes. Subjects who responded to
FMT demonstrated a larger proportion of fungal and bacterial OTUs
that were transferred and predominated in the feces of recipients
after FMT, compared to those who did not respond (Mann-Whitney
test, p=0.0068 and 0.0164 respectively for comparison of
donor-derived fungal and bacterial OTU ratios in recipients, FIG.
2a, b). The community structure at the genus level showed a higher
abundance of the genera Aspergillus and Penicillum in FMT
responders than in non-responders (FIG. 2c). In contrast, the
genera Candida and Simplicillium were significantly enriched in FMT
non-responders. Analogously, a similar pattern was observed at the
bacterial community structure. FMT responders displayed bacterial
abundance resembling that of the donor, whereas FMT non-responders
showed inadequate abundance of donor-enriched bacteria at the last
follow-up post FMT (FIG. 7). Of note, in recipients FMT12 and
FMT16, bacterial configurations at the last follow-up after FMT
were similar to that of healthy controls, but their gut mycobiota
configurations differed significantly from that of healthy
controls. These data indicate that restoration of the gut mycobiota
is at least as important as, if not more than, restoration of the
bacterial microbiota in CDI recipients. Taken together, these data
indicate that the final proportion of donor-derived fungal and
bacterial taxa and alterations of the fecal fungal composition in
the recipient post FMT were associated with treatment outcome of
FMT.
FMT Alters the Gut Mycobiota Distinct from Antibiotic Treatment
[0061] CDI subjects who responded to FMT showed a significant
increase in fungal richness and diversity (Wilcoxon matched-pairs
singed rank test, p=0.0273 and p=0.0474 respectively, FIG. 3a, b).
Although baseline bacterial diversity, evenness and richness were
significantly lower in CDI subjects compared to controls
(Mann-Whitney test, all p<0.0001, FIG. 6a), after FMT there was
a significant increase in bacterial richness (Wilcoxon
matched-pairs singed rank test, p=0.019) and a marginally
significant increase (Wilcoxon matched-pairs singed rank test,
p=0.098) in bacterial diversity in FMT responders. During post-FMT
follow-up, there were profound differences in the gut mycobiota
configurations across different donor-recipient pairs, however a
significantly higher prevalence of the genus Candida was observed
across the serial post-FMT fecal samples of FMT non-responders
relative to that of responders (FIG. 3c, d). In contrast, the
genera Saccharomyces and Aspergillus were present in higher
abundances in FMT responders than in non-responders (FIG. 3c, d).
Discriminative analysis identified disparately presented taxa
between post-FMT samples of FMT responders and non-responders, at
the genus and species levels (FIG. 3d). C. albicans was the most
prominent species enriched after FMT in non-responders.
[0062] C. albicans markedly decreased after FMT (Wilcoxon
matched-pairs singed rank test, p=0.0458, FIG. 3d, e).
Interestingly, both the abundance of C. albicans in donor feces and
in post-FMT recipient fecal samples were associated with FMT
treatment outcome. FMT recipients transplanted with a donor feces
with C. albicans<0.1% in the fungal community achieved a
response to FMT treatment, compared to those transplanted with a
donor feces with C. albicans>0.1% (Chi-square test p=0.049, FIG.
3f). Recipients with an initial high abundance of C. albicans
before FMT and continuing to have a relative abundance of C.
albicans>10% after FMT all experienced a disease recurrence
after FMT (Chi-square test p=0.029, FIG. 3g). These data indicate
that the presence of C. albicans compromises FMT efficacy. The
absolute abundance of C. albicans (in fecal input DNA) was markedly
decreased after FMT in FMT responder group (Wilcoxon matched-pairs
singed rank test, p=0.0391, FIG. 16). Interestingly, FMT
non-responders exhibited significantly higher post-FMT fecal C.
albicans levels in absolute abundance than FMT responders
[Mann-Whitney test, p=0.0018, Log.sub.10 transformed effect size
3.05 (95% CI: 1.48-4.29), FIG. 16], indicating C. albicans can be a
marker for disease recurrence and/or pathogenesis.
[0063] The effect of antibiotics on the gut mycobiota was also
assessed across longitudinal time-points in 8 CDI subjects treated
with vancomycin (STD treatment, FIG. 6, Table 1). Five of the eight
subjects remained symptom-free with a negative stool C. difficile
toxin at the last follow-up (termed responders, STD1-STD5), while
three developed recurrence of CDI (termed non-responders,
STD6-STD8). Unlike FMT, vancomycin induced inconsistent alterations
in the fungal richness and diversity during longitudinal follow-up
(FIG. 9a, b). There was no significant difference in the fungal
richness or diversity between STD responders and non-responders
after FMT, although vancomycin resulted in a significant increase
in bacterial diversity in responders after FMT (FIG. 10, Wilcoxon
matched-pairs singed rank test, p=0.0198).
[0064] FMT and vancomycin led to an increase in the gut fungal
diversity in 81.3% (13 out of 16) and 37.5% (3 out of 8) of CDI
subjects, respectively (Chi-square test p=0.032, FIG. 8c), and an
increase in the gut fungal richness in 68.8% (11 out of 16) and
37.5% (3 out of 8) of CDI subjects, respectively (FIG. 8c). FMT
responders showed a significantly higher fold-change post FMT in
both fungal richness and fungal diversity compared to STD
responders (Mann-Whitney test p=0.019 and Chi-square test p=0.05
respectively, FIG. 8d, e). Collectively, these data indicate that
FMT may be more influential in orchestrating the gut mycobiota than
antibiotics.
[0065] Taxonomical analysis was performed to further elaborate the
effect of antibiotics on the fungal community and to discern
differences between FMT and antibiotics in modulating the gut
mycobiota. After vancomycin treatment, fungal compositions
exhibited similar configurations during follow-up across STD
subjects, with a marked expansion of the genus Candida (FIG. 8f).
To define differentially enriched fungal taxa between subjects who
responded to FMT and vancomycin, we implemented LefSe analysis
across all follow-up samples of treatment responders. FMT treatment
enriched the genera Saccharomyces and Cryptococcus in those who
responded, whereas vancomycin disparately enriched a panel of
fungal genera in STD responders after treatment, which included
Candida, Talaromyces, Erythrobasidium, Periconia, Stemphylium,
Ganoderma (FIG. 11). At the family level, FMT caused an enrichment
of Saccharomycetacean and Herpotrichiellaceae, while vancomycin
caused an enrichment of Intertae sedis (FIG. 11). There was no
statistically significant difference in the relative abundance of
C. albicans between STD responders and non-responders, however a
decrease in C. albicans was seen in STD responders after vancomycin
treatment (FIG. 12). Subject STD7 who had a post-STD relative
abundance of C. albicans>10% developed CDI recurrence after
vancomycin treatment, further substantiating the importance of
alleviation of C. albicans for eradicating CDI.
Trans-kingdom Interactions Between Gut Mycobiota and Bacterial
Microbiota are Associated with Treatment Outcome
[0066] To characterize the ecological network of the gut mycobiota
and bacterial microbiota, the correlation of the a-diversity
(diversity, evenness and richness) of the fungal community with
that of the bacterial community was evaluated. Among the
post-treatment samples of FMT responders, significant positive
correlations were found between fungal diversity and bacterial
diversity, and between fungal richness and bacterial diversity,
evenness, and richness (Spearman's correlation, permutation test,
P<0.05, FIG. 13). In the post-treatment samples of FMT
non-responders and STD responders, the correlation between
bacterial and fungal communities showed a depletion of correlations
between fungal richness and other bacterial and fungal .alpha.
diversity indexes. The correlations were completely abolished
across the post-treatment samples of STD non-responders. The
correlations of fungal genera with bacterial genera were further
assessed in controls and CDI subjects in association with treatment
response. Significant inverse correlations between control-enriched
bacteria, including butyrate-producing Roseburia, and CDI-enriched
Candida were observed in FMT responders and STD responders after
treatment, paralleling a prevalence of positive correlations
between control-enriched bacteria and control-enriched fungi among
which correlation of Roseburia and Aspergillus was present in both
FMT responders and STD responders (FIG. 14). However, those who did
not respond to either FMT or STD displayed an apparent contraction
in the number of fungal-bacterial correlations after treatment,
compared to FMT responders and STD responders. These data suggest
the importance of restoration of an intricate and homeostatic
fungal-bacterial ecosystem in maintaining treatment response.
C. albicans Compromises FMT Efficacy in a Murine Model of CDI
[0067] C. albicans was the most prominent species associated with
treatment failure of FMT in CDI, suggesting a possible causal
relationship. This assumption is further supported by reports
whereby CDI recurrence was observed after antibiotics
treatment.sup.3,19, as antibiotics contribute to the expansion of
Candida. To determine the causal relationship between C. albicans
and response to FMT, the efficacy of FMT in eliminating C.
difficile was assessed using a C. difficile induced-diarrhea murine
model in three groups of mice: (i) mice infused with human stool
preparation, (ii) mice colonized with C. albicans then infused with
human stool preparation, and (iii) mice infused with human stool
preparation supplemented with C. albicans during fecal
transplantation (FIG. 4a). FMT was effective in ameliorating
diarrhea, intestinal inflammation, and decreasing C. difficile
burden, compared to CDI group, while no difference in C. difficile
load was observed among all groups before FMT (FIG. 4b-d). However,
mice that was colonized with C. albicans prior to FMT or those
infused with donor stool supplemented with C. albicans suffered
significant diarrhea, intestinal inflammation, and augmented C.
difficile burden post FMT, when compared with mice administered
with a single infusion of human stool (FIG. 4b-d). There were high
levels of C. albicans in these recipient mice on day 1 post FMT,
though a decrease in C. albicans load was observed after FMT in
mice colonized with C. albicans prior to FMT (FIG. 4e). An
anti-fungal agent, fluconazole, to eradicate C. albicans in a group
of recipient mice prior to human stool infusion (FMT) (FIG. 15a).
C. difficile load was then compared after human stool infusion
between mice with and without anti-fungal treatment. Anti-fungal
treatment in recipient mice colonized with C. albicans before human
stool infusion restored the efficacy of FMT in clearing C.
difficile infection (FIG. 15b). These data demonstrate that the
existence of C. albicans, either in the recipient or in the donor,
negates the efficacy of FMT in clearing C. difficile, while
antifungal treatment reestablishes its efficacy. These results
highlight that persistent fungal dysbiosis with aberrant presence
of C. albicans can confer an unfavourable FMT outcome in CDI.
Total Fungal Load is Increased in CDI
[0068] The total fecal fungal load was significantly higher in CDI
than in controls [Mann-Whitney test, p=0.0004, Log.sub.log
transformed effect size 1.32 (95% CI: 0.62-1.97), FIG.17].
Total Fungal Load and C. albicans in Inflammatory Bowel Disease
(IBD)
[0069] The total fecal fungal load was significantly higher in
patients with IBD, including patients with Crohn's disease (CD) and
Ulcerative colitis (UC)--two subtypes of IBD, than in controls
(Mann-Whitney test, p=0.0003, p=0.0225, respectively, FIG. 18a).
The fecal presence ratio of C. albicans is higher in CD than in
controls, and 3 CD patients exhibiting the highest C. albicans
levels had a history of recent exposure to antibiotics (FIG.
18b).
Total Fungal Load is Increased in Irritable Bowel Syndrome
(IBS)
[0070] The total fecal fungal load was significantly higher in IBS
than in controls [Mann-Whitney test, p=0.0237, FIG.19].
Bacterial Alterations in CDI after FMT in Association with FMT
Outcome
[0071] The present inventors explored the composition of the
bacterial microbiota after FMT in relation to FMT outcomes, at
various taxonomic levels (FIG. 20). Actinobacteria, Bacteroidetes
(phylum-level taxa), Lachnospiraceae, Clostridiaceae, and
Ruminococcaceae (family-level taxa), Clostridium, Blautia, and
Faecalibacterium (genus-level taxa) were significantly more
enriched in FMT responders than in non-responders after FMT.
However, bacteria from the phylum Proteobacteria were more abundant
in FMT non-responders relative to FMT responders. FMT responders
displayed bacterial abundances resembling that of the donor,
whereas FMT non-responders showed inadequate relative abundances of
donor-enriched bacteria at the last follow-up after FMT (FIG. 20c).
LefSe analysis on the fecal bacteriomes of donors at the genus
level identified Escherichia and Proteus as the differentially
enriched genera in FMT responders' donor stool and in FMT
non-responders' donor stool respectively (LDA effect size 2.58 and
2.35, FDR adjusted q=0.017 and 0.006, respectively).
Discussion
[0072] This is the first study to characterize the gut mycobiota in
CDI and to elucidate mycobiota alterations after FMT in relation to
treatment outcome. Patients with CDI showed enteric fungal
dysbiosis. Importantly, disease recurrence after FMT was associated
with several important findings including persistent fungal
dysbiosis, low levels of donor-derived fungal colonization, high
abundance of C. albicans in the recipient stool before FMT and the
presence of C. albicans in the donor stool. The observations that
disease cure requires both fungal and bacterial colonization from
the donor provides new and important insights into the potential
therapeutic importance of the gut mycobiota in treatment outcome in
FMT, beyond the bacterial microbiota. These data also highlight a
new concept in FMT that the abundance of fecal C. albicans both in
recipient before treatment and in donor are critical components
when considering implementation of FMT. Integration of more
in-depth mycobiota analysis in donor-recipient pair may lead to
personalized and targeted gut microbial therapy in the future.
[0073] Although studies of the gut mycobiota have lagged behind
that of the gut bacterial microbiota, fungi are increasingly being
considered as important players of the gut and interactions between
pathogenic and commensal fungal and bacterial communities are
crucial in the maintenance of human health and disease
pathogenesis.sup.16. Furthermore, disruption of the gut mycobiota
has deleterious effect on host immunity.sup.17. Despite high
interpersonal variability of the gut mycobiota in patients with
CDI, there was a significant expansion of the genus Candida and the
species C. albicans. Interestingly, FMT induced an increase in the
genus Saccharomyces along with a marked contraction of Candida and
C. albicans after FMT in treatment responders, while recipients who
demonstrated high abundance of C. albicans in the stool after FMT
(10%) or those whose donor had a high abundance of (0.1%) of C.
albicans were more likely to have disease recurrence after FMT.
Over-presentation of C. albicans in the recipient stool after FMT
may largely contribute to treatment failure.
[0074] The role of fungal commensals in educating the human immune
system has gained new appreciation in intestinal disease. In the
steady state, bacterial communities keep fungi in check in the gut.
Fungi are major causes of infections among immunocompromised or
hospitalized patients with serious underlying diseases and
comorbidities. Candida species remain the most important cause of
opportunistic infections worldwide, affecting predominantly elderly
patients.sup.20. Candidalysin was recently unveiled as a fungal
toxin from C. albicans critical for mucosal infection.sup.21.
Commensal bacteria inhibit C. albicans colonization through
activation of HIF-1.alpha. and LL-37.sup.22. Antibiotic treatment
selectively and effectively eradicates the bacterial community but
consequently leads to fungal outgrowth, particularly the Candida
species.sup.23,24. Antibiotics or immunosuppressants are effective
in the short term but they likely compromise the immune system in
the longer term. A compromised immune system creates a more
favourable environment to expansion of Candida and overgrowth of
Candida can alter the recovery of the gut bacterial microbiota
after cessation of antibiotic treatment .sup.25,26. In this study,
the over-presence of Candida species in recipients might account
for the high failure rate of FMT in CDI. In DSS-induced colitis
mouse model as well as patients with inflammatory bowel disease
(IBD), C. albicans and Candida were significantly
enriched.sup.27-29. Antifungal treatment decreased Candida
prevalence and ameliorated inflammatory responses in DSS colitis
mice.sup.29. However, disruption of fungal communities by long-term
use of antifungals aggravated severity of DSS colitis and allergic
airway.sup.30. Collectively, these data implicate the importance of
the gut fungal-bacterial homeostasis in host health. These data
suggest that the establishment of a balanced gut fungal and
bacterial community via FMT is important to eradicate CDI, as FMT
non-responders showed abrogated fungi-bacteria correlations in
a-diversity and taxa when compared with responders.
[0075] In conclusion, gut mycobiota alterations may determine
treatment outcome in FMT. The persistence of fungal dysbiosis,
particularly the presence of C. albicans, can incur CDI recurrence.
The findings disclosed herein highlight the importance of both
"optimal" donor selection and pre-FMT eradication of C. albicans in
recipient during FMT practice, where future FMT therapy should
incorporate detailed characterization and stratification of both
donor and recipient fecal mycobiomes. These results provide a
framework for future investigations into the contribution of
donor/recipient mycobiota profiles and gut fungi-bacteria
interactions in FMT treatment for various human diseases.
Methods
[0076] Study subjects and treatment outcome
[0077] The current study was a sub-study from a randomised
controlled trial (RCT) of FMT versus vancomycin (standard therapy,
STD) for patients with CDI. Consecutive CDI subjects enrolled into
this randomised controlled trial were invited to participate in a
substudy of assessment of fecal microbiota. Patients were included
if they had three or more loose or watery stools per day for at
least two consecutive days or eight or more soft or loose stools in
48 hours and a positive stool test for C. difficile based on a
two-step testing algorithm in our hospital, a positive GDH
(Glutamate dehydrogenase) screening test followed by a positive
polymerase chain reaction (PCR) test of C. difficile. A total of 31
subjects with CDI and 24 healthy household controls were recruited
and stool samples at baseline were obtained for analyses of fungal
and bacterial microbiomes. Among them, 24 CDI subjects consented to
have stool samples collected serially after treatment for
microbiome analysis. 16 CDI subjects were treated with FMT and 8
were treated with vancomycin, and they were followed up at baseline
and at weeks 2, 4, 10 and 16 after treatment (FIG. 6). Subjects in
the FMT group received 5 days of vancomycin followed by donor
infused stool via nasojejunal route and those who had STD received
oral vancomycin 500 mg orally four times per day for 10 days. A
computer-generated randomization schedule was used to assign
patients to the treatment sequences. All patients kept a stool
diary and were questioned about stool frequency and consistency and
medication use.
[0078] Treatment response was defined as an absence of diarrhea or
persistent diarrhea that could be explained by other causes with a
negative stool test for C. difficile toxin, while relapse was
defined as diarrhea with a positive stool test for C. difficile
toxin. Treatment cure is defined as symptom resolution and a
negative Clostridium difficile toxin in stool until the last
follow-up (last follow-up is referred to as the last stool
collection time point, as shown in FIG. 6). 9 of the 16 subjects
who had FMT (FMT1-FMT9), and 5 of the 8 patients (STD1-STD5), who
had vancomycin were cured of CDI (termed responders, Table 1) at a
median follow-up of 16 weeks. CDI recipients FMT11 and FMT12 shared
the same donor, and this donor was termed "Donor11". Clinical data
of the subjects and collected stool samples are shown in Table 3.
None of the patients had received antibiotics or proton pump
inhibitors after FMT.
Study design Patient inclusion criteria:
[0079] 1. C. difficile infection was defined as diarrhea (.gtoreq.3
soft, loose or watery stools per day for at least 2 consecutive
days or .gtoreq.8 soft or loose stools in 48 hours) and a positive
stool test for C. difficile toxin; and
[0080] 2. Age.gtoreq.18; and
[0081] 3. Written informed consent obtained
Patient exclusion criteria:
[0082] 1. The presence of human immunodeficiency virus (HIV)
infection with a CD4 count of less than 240
[0083] 2. Pregnancy
[0084] 3. GI Bleeding
[0085] 4. Acute coronary syndrome
Donor screening:
[0086] Donors included individuals who are spouses or partners,
first-degree relatives, other relatives, friends, and individuals
unknown to the patient. They were screened with a questionnaire and
excluded if they had taken antibiotics within the preceding 3
months; were on major immunosuppressive agents, including
chemotherapeutic agents; had known or recent exposure to HIV,
hepatitis B or C; had a current communicable disease; participated
in high-risk sexual behaviors; used illicit drugs; traveled within
6 months to areas with endemic diarrheal illnesses; or had history
of inflammatory bowel disease, irritable bowel syndrome or chronic
diarrhea, gastrointestinal malignancy or polyposis. In addition,
donor was screened for HBsurface Ag, Anti-HBc, Anti-HCV, Anti-HIV,
Syphilis EIA, stool microscopy, culture and sensitivity, stool
cyst, ova, parasite, norovirus and C. difficile (cytotoxin and PCR
assay). All subjects and collected stool samples are listed in
Table 1.
[0087] The donors for the FMT group were healthy household controls
and the donor stool samples analyzed were the same samples used for
FMT. All subjects provided written informed consent.
[0088] Family members provided donor stool for subjects randomised
to FMT arm. Cure after FMT or vancomycin therapy was defined as
symptom resolution and negative Clostridium difficile toxin in
stool at last follow-up by PCR assay. Relapse was defined as
diarrhea with a positive stool test for C. difficile toxin.
[0089] This was a randomised but not blinded study. However for
mycobiome and bacterial microbiome analyses on stool samples,
assessments were initially performed by analysts who were blinded
to the clinical outcome of the studied subjects. When the profiled
mycobiome and bacterial microbiome data were available for each
individual subject, correlation was then made to associate
microbiome profiles with treatment outcomes of subjects.
Infusion of Donor Stool
[0090] In subjects who received FMT, a nasoduodenal tube was
inserted with radiology guidance. Donor feces was diluted with 500
ml of sterile saline (0.9%), blended and the supernatant was
strained with filter paper and poured in a sterile bottle. Within 6
hours after collection of feces by the donor, the solution was
infused through a nasoduodenal tube (2 to 3 minutes per 50 ml). The
tube was removed 30 minutes after the infusion, and patients were
monitored for 2 hours. In subjects with received FMT, a minimum of
50g of donor stool was collected on the same day of infusion and
used within 6 hours of collection.
Fecal DNA Extraction
[0091] Fecal DNA was isolated as described below. 100 mg fecal
sample was pre-washed with 1 ml ddH.sub.2O and pelleted by
centrifugation at 10,000.times.g for 1 minute. The fecal pellet was
re-suspended in 800 .mu.l TE buffer (pH 7.5), supplemented with 1.6
.mu.l 2-Mercaptoethanol and 500 U lyticase (Sigma), and incubated
at 37.degree. C. for 60 min. The sample was then centrifuged at
10,000.times.g for 2 minutes and fecal DNA was subsequently
extracted from the pellet using ZR Fecal DNA miniPrep kit (Zymo
Research, Orange, Calif.) according to the protocol. Briefly, fecal
pellet was added to the BashingBeadLysis Tube with 750 .mu.l Lysis
solution, and then processed at maximum speed for 10 minutes. The
lysates were centrifugeed at .gtoreq.10,000.times.g for 1 minute.
The supernatant was transferred to a Zymo-Spin.TM. IV Spin Filter
in a collection tube and centrifuged at 7,000.times.g for 1 minute.
About 1,200 .mu.l of fecal DNA binding buffer was added to the
filtrate in the collection tube, followed by concentration and
purification in a new filter tube. Finally, a total of 50 .mu.l
eluted DNA with a concentration at 20-100 ng/.mu.l was prepared for
each sample.
Fungal ITS2 Sequencing and Quality Control
[0092] The final fecal DNA for fungal sequencing was amplified
based upon ITS2 (Internal transcribed spacer 2) region using
primers as below and PrimeSTAR HS DNA Polymerase kit (TaKaRa,
Japan). The primer pairs are ITS2-F: 5'-GCATCGATGAAGAACGCAGC-3',
ITS2-R: 5'-TCCTCCGCTTATTGATATGC-3'. ITS2 amplicons were generated
with 38 cycles of 3-step PCR: 98.degree. C. 10 s, 59.degree. C. 10
s, and 72.degree. C. 30 s. PCR samples were then sequenced on the
Illumina MiSeq PE300 platform (2.times.300 bp, BGI, China),
151,524.+-.97,694 (number.+-.SD) clean sequences obtained on
average (sequence statistics in Table 4).
[0093] Raw reads were filtered by SOAPnuke (v 1.5.3) (web site:
soap.genomics.org.cn/) developed by BGI as follows: (i) adaptors
removed, (ii) read removed if N base is more than 3% of the read,
(iii) read removed if bases with quality low than 20 were more than
40% of read, (iv) all duplicates removed. Quality control and data
analysis were further implemented in PIPITS (v 1.4.5).sup.31.
Briefly, PIPITS_PREP prepares raw reads from Illumina MiSeq
sequencers for ITS extraction; PIPITS_FUNITS extracts ITS2 from the
reads; and PIPITS_PROCESS analyses the reads to produce operational
taxonomic unit (OTU) abundance tables and the RDP taxonomic
assignment table for downstream analysis. The quality trimmed and
ITS2 extracted reads were aligned to fungi UNITE database
exploiting RDP classifier 2.10 for taxonomic assignment to produce
operational taxonomic unit (OTU) abundance table (based on sequence
identity.gtoreq.97% identity) and phylotype abundance tables at
different taxonomic levels, for downstream analysis.
[0094] The fungal OTU and phylptype abundance data were imported
into R 3.2.3. Richness, diversity, and evenness calculation were
performed using the estimated richness function of the phyloseq
package. Spearman correlation and their significance were
calculated using the cor and cor.test functions in R, respectively.
For the fungal-bacterial taxa comparisons, Spearman correlations
were calculated for the relative abundance of the differentially
presented fungal taxa and the bacterial taxa determined to be
significantly associated with disease by Lefse analysis.
Correlation plots were generated using the corrplot package in R.
Heat maps were generated using the pheatmap package in R.
Quantitative PCR for Detection of C. albicans in Human Fecal DNA
Samples
[0095] C. albicans loads in human stools were quantified by qPCR
analysis (SsoAdvanced SYBR Green Supermix, Bio-Rad) of extracted
human fecal DNA using C. albicans specific primers: C. albicans-F
5'-CCTGTTTGAGCGTCGTTTCTC-3'; C. albicans-R
5'-TTTGGTTAGACCTAAGCCATTGTCA-3'. C. albicans abundance was
determined using standard curves constructed with reference genomic
DNA (gDNA) of C. albicans.
Quantitative PCR for Detection of Total Fungal Load in Human Fecal
DNA Samples
[0096] Total fungal loads in human stools were quantified by TaqMan
qPCR analysis (Premix Ex Taq.TM., TaKaRa) of extracted human fecal
DNA using primers.sup.36: Fungi-quant-F 5'-GGRAAACTCACCAGGTCCAG-3';
Fungi-quant-R 5'-GSWCTATCCCCAKCACGA-3', and probe:
5'-TGGTGCATGGCCGTT-3'.
LEfSe Linear Discriminant Analysis
[0097] To compare differences in the configurations of fungal and
bacterial microbiomes between CDI patients and healthy controls,
between FMT responders and non-responders, between FMT treatment
and vancomycin (STD) treatment, Lefse analyses were performed on
the Huttenhower lab Galaxy server (web site:
huttenhower.sph.harvard.edu/galaxy/) by importing the viral and
bacterial relative abundance values and associated sample metadata,
with FDR adjusted p value<0.05 considered significant and effect
size calculated.
Calculation of Donor Transferred OTUs in Recipients
[0098] In samples after FMT, if a fungal or bacterial OTU was not
present in the recipient baseline sample but present both in the
corresponding donor baseline sample and in the recipient post-FMT
sample, the OTU was defined as "donor derived"; if an OTU was not
present in the corresponding donor baseline sample but detected
both in the recipient baseline sample and in the recipient post-FMT
sample, the OTU was defined as "recipient exclusive"; if an OTU was
present across the recipient baseline sample, the recipient
post-FMT sample and the corresponding donor baseline sample, the
OTU was defined as "donor-recipient co-existed."
Bacterial 16S rRNA Sequencing and Data Analysis
[0099] The final fecal DNA samples were subject to bacterial 16S
rRNA V4 region amplification and sequenced on the Illumina MiSeq
PE250 platform (2.times.250 bp, BGI, China), 132,081.+-.65,429
(number.+-.SD) sequences obtained on average (sequence statistics
in Table 5). Quality control and data analysis were implemented in
mothur (v 1.38.0) as previously described.sup.32. Any sequences
with ambiguous bases and anything longer than 275 bp were removed,
and aligned against the non-redundant Greengenes database (v
13.8).sup.33 using the NAST algorithm. Any sequences that failed to
align with the V3-4 region were discarded. The remaining sequences
were trimmed to the same alignment coordinates over which they
fully overlapped, followed by removal of homopolymers and detection
for the presence of chimeras by UChime.
[0100] The resulting sequences were classified against the
Greengenes database and annotated with deepest level taxa
represented by pseudo-bootstrap confidence scores of at least 80%
averaged over 1,000 iterations of the naive Bayesian classifier.
Any sequences that were classified as either being originated from
archaea, eukarya, chloroplasts, mitochondria, or unknown kingdoms,
were removed. The annotated sequences were assigned to phylotypes
according to their consensus taxonomy with which at least 80% of
the sequences agreed. Closed reference operational taxonomic units
(OTUs) sharing 97% identity were clustered as well and assigned
taxonomy according to the Greengenes database. Lefse analysis was
performed to define bacterial taxa associated with CDI and healthy
controls. The relative abundance of these abundance-differential
taxa identified by LefSe in pre-FMT baseline samples and post-FMT
last follow-up samples were plotted using pheatmap R package.
[0101] Mouse Husbandry and Model of C. difficile Infection
[0102] Studies were conducted on 4- to 6-week old demale C57BL/6
that were reared in groups of 9. Individual mice were randomized
after arrival. Mice were subjected to a previously described model
of CDI.sup.34. Briefly, mice were given an antibiotic cocktail of
kanamycin (0.4 mg/mL), gentamicin (0.035 mg/mL), colistin (850
U/mL), metronidazole (0.215 mg/mL), and vancomycin (0.045 mg/mL)
(all antibiotics were purchased from Sigma-Aldrich, St. Louis, Mo.)
in their drinking water for 3 days. Mice were then given 2 days of
recovery before administration of 10.sup.7 spores of C. difficile
in PBS via oral gavage. Animal grouping and research scheme were
designed as shown in FIG. 4a. On day 1 post stool infusion,
diarrhea was evaluated by stool water content, calculated as stool
weight loss after air drying at 70.degree. C. for 4 hours. Colons
were harvested, fixed in 4% formalin solution and embedded in
paraffin. Sections were stained with hemotoxylin and eosin for
histological assessment.
[0103] For antifungal experiment, animal grouping and research
scheme were designed as shown in FIG. 15a. Mice was initially
colonized with C. albicans (2.times.10.sup.8 cfu per mouse) after 3
days of antibiotic cocktail treatment in the drinking water,
followed by 4 days of fluconazole treatment supplemented in the
drinking water (0.5 mg/mL, Sigma). Then the mice were subjected to
C. difficile administration (10.sup.7 spores per mice) through
gavage after a consecutive 1.5-day antibiotic cocktail- and 1.5-day
free water- drinking. Human stool infusion was performed 2 days
later after C. difficile gavage. Both C. difficile load and C.
albicans load were enumerated by cultivation on Day 0 before FMT
and Day 1 after FMT.
C. albicans Administration and Donor Stool Infusion in Mice
[0104] C. albicans (10231, purchased from ATCC, USA) was
administered to mice (2.times.10.sup.8 cfu per mouse) via gavage
after 3-day antibiotic treatment or supplemented in donor stool
slurry at the time of donor stool infusion. Human stool from a
healthy volunteer (Chinese, male, age 28 years), without presence
of C. albicans as measured by qPCR, was obtained with informed
consent. For stool microbiota infusions, approximately 500 mg of
stool samples were cut in an anaerobic chamber and suspended in 5
ml of phosphate-buffered saline. Mice were colonized by oral gavage
of 150 .mu.l of fecal slurry with or without supplementation of C.
albicans on day 2 after C. difficile challenge.
Quantification of C. difficile and C. albicans Burdens in Mouse
Feces
[0105] Mouse stool were collected both before and after stool
infusion. Fecal C. difficile and C. albicans burdens on day 0
before and day 1 after stool infusion were measured by cultivation.
Samples were diluted in PBS and respectively plated on taurocholate
cycloserine cefoxitin fructose agar (TCCFA) for quantification of
C. difficile burden, on Sabouraud dextrose agar (SDA) for
quantification of C. albicans load. Stool samples prior to C.
albicans colonization from antibiotic-treated mice were plated on
SDA to ensure that mice were C. albicans culture negative.
Data Availability
[0106] Sequence data and accompanying metadata have been deposited
to the NCBI Sequence Read Archive under BioProject accession
numbers PRJNA419097 and PRJNA419104.
Example 2
C. albicans Level Associated with Unfavorable FMT Outcome in
IBD
[0107] The fecal C. albicans level was investigated in three IBD
patients with concurrent CDI, and subsequently followed them up
after FMT (FIG. 21). Disease symptoms were ameliorated soon after
FMT. However, all three patients manifested unfavorable FMT
outcomes at different time-points post FMT. In accordance with the
finding for CDI patients, these IBD patients all showed increased
fecal C. albicans levels after FMT. Taken into consideration the
previous observation that C. albicans levels were also higher in
IBD than in Controls (FIG. 18), it indicates that C. albicans may
play an unfavorable role in IBD and IBD-FMT.
[0108] All patents, patent applications, and other publications,
including GenBank Accession Numbers, cited in this application are
incorporated by reference in the entirety for all purposes.
TABLE-US-00001 TABLE 1 Duration of Outcome Severe/ follow up (till
last Subject Sex Age Smoking Moderate (wks) follow up) FMT1 M 80
Ex-smoker Moderate 16 Cured FMT2 M 52 No Severe 27 Cured FMT3 M 38
No Moderate 17 Cured FMT4 F 76 No Moderate 18 Cured FMT5 M 63 No
Severe 18 Cured FMT6 M 88 No Severe 23 Cured FMT7 M 45 Cured FMT8 M
90 Cured FMT9 F 52 Cured FMT10 M 45 Ex-smoker Severe 20 Recurrence
at week 19 FMT11 F 83 No Moderate 11 Recurrence at week 5 FMT12 F
38 No Severe 28 Recurrence at week 28 FMT13 M 81 Recurrence at week
2 FMT14 M 65 Recurrence at week 2 FMT15 F 90 Recurrence at week 4
FMT16 M 83 Recurrence at week 4 STD1 F 78 smoker Severe 14 Cured
STD2 F 83 No Severe 17 Cured STD3 F 99 No Moderate 26 Cured STD4 F
85 Cured STD5 F 92 Cured STD6 M 88 Ex-smoker Severe 20 Recurrence
at week 12 STD7 M 93 No Moderate 7 Recurrence at week 7 STD8 M 63
Recurrence at week 5
TABLE-US-00002 TABLE 2 Order level lefSe analysis enriched in LDA
effect order group size q value o_Saccharomycetales CDI 5.26234241
0.00141993 o_Incertae_sedis Control 4.22542515 0.01743251
o_Ustilaginales Control 4.64245919 0.01113039 o_Wallemiales Control
3.86277202 0.02933565 o_Eurotiales Control 5.02946522 3.45E-05
o_Trechisporales Control 4.62024264 0.00835961 o_Agaricostilbales
Control 4.77590173 0.04495598 o_Mucorales Control 3.94917134
0.00275496 o_Chaetothyriales Control 3.96218634 0.03957291
o_unidentified Control 4.43843789 0.0011891 Family level lefSe
analysis enriched in LDA effect family group size q value
f_Incertae_sedis CDI 5.361603211 0.00032083 f_Diatrypaceae CDI
3.647432029 0.02396599 f_Lichtheimiaceae Control 3.776192836
0.03565053 f_Marasmiaceae Control 4.460718116 0.04495598
f_Cordycipitaceae Control 3.018060628 0.01080346 f_Trichocomaceae
Control 4.998286724 3.71E-05 f_Monascaceae Control 4.442880786
0.00806352 f_Ustilaginaceae Control 3.308596392 0.01113039
f_Agaricostilbaceae Control 3.637118408 0.04495598 f_unidentified
Control 4.382578512 0.00989125 f_Pichiaceae Control 4.234924293
0.00153938 f_Rhizopodaceae Control 3.759990223 0.03298649
f_Pleosporaceae Control 4.266635541 0.04792052 f_Mucoraceae Control
3.546579211 0.00818474 f_Eremotheciaceae Control 3.514966042
0.01499872 f_Wallemiaceae Control 3.255471141 0.02933565
f_Hydnodontaceae Control 3.172134768 0.01944714 Genus level lefSe
analysis enriched in LDA effect genus group size q value g_Candida
CDI 5.382628239 0.00018873 g_Wallemia Control 3.198526789
0.02933565 g_Trechispora Control 3.55075498 0.01944714 g_Lentinula
Control 3.687563833 0.04495598 g_Alternaria Control 4.061524753
0.00543258 g_Talaromyces Control 3.815612518 0.04495598
g_Aspergillus Control 4.856125262 8.75E-05 g_Pichia Control
3.729764446 0.00177011 g_Thermomyces Control 3.268072407 0.0138274
g_Rhizopus Control 3.612096939 0.03298649 g_unidentified Control
4.780192159 0.00077864 g_Simplicillium Control 2.905283827
0.01080346 g_Monascus Control 4.416347845 0.00806352 g_Mucor
Control 3.444062001 0.00818474 g_Penicillium Control 4.397256252
7.64E-05 g_Sterigmatomyces Control 3.736578962 0.04495598
g_Eremothecium Control 3.41388614 0.01499872 Species level lefSe
analysis enriched in species group LDA effect size q value
s_Candida_albicans CDI 4.883094137 0.0128582 s_Hanseniaspora_sp CDI
2.476489287 0.02396599 s_Penicillium_sp CDI 3.159815075 0.02226132
s_Aspergillus_austroafricanus Control 2.820485891 0.02586708
s_Penicillium_dierckxii Control 3.112142685 0.04495598
s_Eurotiomycetes_sp Control 4.236581004 0.00632072
s_Pseudozyma_churashimaensis Control 2.766834362 0.04495598
s_Thermomyces_lanuginosus Control 3.158801272 0.0138274
s_Ustilaginaceae_sp Control 2.551290082 0.04335394
s_Monascus_purpureus Control 4.436483036 0.00806352
s_Penicillium_brocae Control 3.202903826 0.00668459
s_Wallemia_mellicola Control 3.029805635 0.02933565
s_Sterigmatomyces_halophilus Control 3.158900806 0.04495598
s_Eremothecium_sinecaudum Control 3.158887769 0.04495598
s_Pseudozyma_sp Control 2.865506029 0.04495598
s_Rhodotorula_dairenensis Control 3.652021075 0.04495598
s_Aspergillus_penicillioides Control 3.45521592 0.03459752
s_Ophiostomataceae_sp Control 3.730559216 0.04495598
s_Mucor_racemosus Control 2.732298798 0.00354366
s_Lichtheimiaceae_sp Control 3.462009204 0.04495598
s_Penicillium_steckii Control 2.538239261 0.03894247
TABLE-US-00003 TABLE 3 FMT/STD Randomization sample_name number
baseline_comparasion Sample_collection sample_number# arm F1W0 FMT1
CDI longitudinal 3 FMT F1W2 FMT1 NA longitudinal 4 FMT F1W6 FMT1 NA
longitudinal 5 FMT Control1 Donor1 Control cross-sectional 6 Donor
F2W0 FMT2 CDI longitudinal 9 FMT F2W2 FMT2 NA longitudinal 10 FMT
F2W4 FMT2 NA longitudinal 11 FMT Control2 Donor2 Control
cross-sectional 13 Donor F3W0 FMT3 CDI longitudinal 18 FMT F3W2
FMT3 NA longitudinal 19 FMT F3W4 FMT3 NA longitudinal 20 FMT F3W10
FMT3 NA longitudinal 21 FMT F3W17 FMT3 NA longitudinal 22 FMT
Control3 Donor3 Control cross-sectional 23 Donor F4W0 FMT4 CDI
longitudinal 26 FMT F4W2 FMT4 NA longitudinal 27 FMT F4W4 FMT4 NA
longitudinal 28 FMT F4W5 FMT4 NA longitudinal 29 FMT F4W18 FMT4 NA
longitudinal 31 FMT Control4 Donor4 Control cross-sectional 32
Donor F5W0 FMT5 CDI longitudinal 38 FMT F5W2 FMT5 NA longitudinal
39 FMT F5W10 FMT5 NA longitudinal 40 FMT F5W18 FMT5 NA longitudinal
41 FMT Control5 Donor5 Control cross-sectional 42 Donor F6W0 FMT6
CDI longitudinal 43 FMT F6W2 FMT6 NA longitudinal 44 FMT F6W11 FMT6
NA longitudinal 46 FMT Control6 Donor6 Control cross-sectional 47
Donor F7W0 FMT7 CDI longitudinal 89 FMT F7W4 FMT7 NA longitudinal
90 FMT F7W12 FMT7 NA longitudinal 91 FMT Control7 Donor7 Control
cross-sectional 78 Donor F8W0 FMT8 CDI longitudinal 83 FMT F8W20
FMT8 NA longitudinal 106 FMT Control8 Donor8 Control
cross-sectional 79 Donor F9W0 FMT9 CDI longitudinal 127 FMT F9W2
FMT9 NA longitudinal 128 FMT F9W4 FMT9 NA longitudinal 129 FMT
Control9 Donor9 Control cross-sectional 130 Donor F10W0 FMT10 CDI
longitudinal 50 FMT F10W2 FMT10 NA longitudinal 51 FMT F10W6 FMT10
NA longitudinal 52 FMT F10W10 FMT10 NA longitudinal 53 FMT
Control10 Donor10 Control cross-sectional 54 Donor F11W0 FMT11 CDI
longitudinal 62 FMT F11W2 FMT11 NA longitudinal 63 FMT F11W4 FMT11
NA longitudinal 64 FMT Control11 Donor11 Control cross-sectional 65
Donor F12W0 FMT12 CDI longitudinal 66 FMT F12W4 FMT12 NA
longitudinal 68 FMT F12W10 FMT12 NA longitudinal 69 FMT F13W0 FMT13
CDI longitudinal 82 FMT F13W2 FMT13 NA longitudinal 102 FMT F13W13
FMT13 NA longitudinal 103 FMT Contal13 Donor13 NA longitudinal 80
Donor F14W0 FMT14 CDI longitudinal 113 FMT F14W4 FMT14 NA
longitudinal 115 FMT Contal14 Donor14 Control cross-sectional 119
FMT F15W0 FMT15 CDI longitudinal 131 FMT F15W2 FMT15 NA
longitudinal 132 FMT F15W4 FMT15 NA longitudinal 133 FMT Contal15
Donor15 Control cross-sectional 134 Donor F16W0 FMT16 CDI
longitudinal 7 FMT F16W4 FMT16 NA longitudinal 86 FMT Contal16
Donor16 Control cross-sectional 8 Donor S1W0 STD1 CDI longitudinal
33 Std therapy S1W2 STD1 NA longitudinal 34 Std therapy S1W4 STD1
NA longitudinal 35 Std therapy S2W0 STD2 CDI longitudinal 58 Std
therapy S2W2 STD2 NA longitudinal 59 Std therapy S2W4 STD2 NA
longitudinal 60 Std therapy S2W10 STD2 NA longitudinal 61 Std
therapy S3W0 STD3 CDI longitudinal 71 Std therapy S3W2 STD3 NA
longitudinal 72 Std therapy S3W4 STD3 NA longitudinal 73 Std
therapy S4W0 STD4 CDI longitudinal 81 Std therapy S4W2 STD4 NA
longitudinal 98 Std therapy S4W10 STD4 NA longitudinal 100 Std
therapy S5W0 STD5 CDI longitudinal 107 Std therapy S5W2 STD5 NA
longitudinal 108 Std therapy S5W4 STD5 NA longitudinal 109 Std
therapy S5W10 STD5 NA longitudinal 110 Std therapy S5W13 STD5 NA
longitudinal 111 Std therapy S5W23 STD5 NA longitudinal 112 Std
therapy S6W0 STD6 CDI longitudinal 14 Std therapy S6W2 STD6 NA
longitudinal 15 Std therapy S6W10 STD6 NA longitudinal 17 Std
therapy S7W0 STD7 CDI longitudinal 24 Std therapy S7W2 STD7 NA
longitudinal 25 Std therapy S8W0 STD8 CDI longitudinal 120 Std
therapy S8W4 STD8 NA longitudinal 121 Std therapy CDI25 NA CDI
cross-sectional 1 NA Control12 NA NA cross-sectional 2 NA CDI26 NA
CDI cross-sectional 88 NA Control17 NA Control cross-sectional 37
NA CDI27 NA CDI cross-sectional 48 NA Control18 NA Control
cross-sectional 49 NA CDI28 NA CDI cross-sectional 55 NA Control19
NA Control cross-sectional 57 NA CDI29 NA CDI cross-sectional 70 NA
CDI30 NA CDI cross-sectional 74 NA Control20 NA Control
cross-sectional 75 NA DI31 NA CDI cross-sectional 122 NA CDI32 NA
CDI cross-sectional 124 NA Control21 NA Control cross-sectional
ANS2357 NA Control22 NA Control cross-sectional ANS2331 NA
Control23 NA Control cross-sectional ANS2237 NA Control24 NA
Control cross-sectional ANS2467 NA time_point_post_treatment donor
House Collect (FMT/STD, relationship hold ID sample_name Date week)
Age Sex to patient (family ID) F1W0 27 Oct. 2015 0 80 M A F1W2 16
Nov. 2015 2 A F1W6 14 Dec. 2015 6 A Control1 27 Oct. 2015 35 F
Daughter A F2W0 13 Feb. 2015 0 52 M B F2W2 6 Mar. 2015 2 B F2W4 20
Mar. 2015 4 B Control2 12 Feb. 2015 51 F Wife B F3W0 20 Mar. 2015 0
38 M C F3W2 14 Apr. 2015 2 C F3W4 28 Apr. 2015 4 C F3W10 2 Jun.
2015 10 C F3W17 28 Jul. 2015 17 C Control3 20 Mar. 2015 73 M Father
C F4W0 3 Jun. 2015 0 76 F D F4W2 20 Jun. 2015 2 D F4W4 7 Jul. 2015
4 D F4W5 13 Jul. 2015 5 D F4W18 16 Oct. 2015 18 D Control4 1 Jun.
2015 53 F Daughter D F5W0 30 Jul. 2015 0 63 M E F5W2 18 Aug. 2015 2
E F5W10 19 Oct. 2015 10 E F5W18 14 Dec. 2015 18 E Control5 31 Jul.
2015 36 F E F6W0 21 Aug. 2015 0 88 M F F6W2 17 Sep. 2015 2 F F6W11
20 Nov. 2015 11 F Control6 24 Aug. 2015 41 M Son F F7W0 1 Feb. 2016
0 45 M G F7W4 7 Mar. 2016 4 G F7W12 9 May 2016 12 G Control7 8 Jan.
2016 35 M No G relationship F8W0 21 Jan. 2016 0 90 M H F8W20 20
Jun. 2016 20 H Control8 22 Jan. 2016 36 F granddaughter H F9W0 15
Sep. 2016 0 52 F I F9W2 30 Sep. 2016 2 I F9W4 14 Oct. 2016 4 I
Control9 9 Sep. 2016 28 F No I relationship F10W0 26 Aug. 2015 0 45
M J F10W2 22 Sep. 2015 2 J F10W6 22 Oct. 2015 6 J F10W10 18 Nov.
2015 10 J Control10 2 Sep. 2015 21 M Son J F11W0 30 Sep. 2015 0 83
F K F11W2 18 Oct. 2015 2 K F11W4 4 Nov. 2015 4 K Control11 25 Sep.
2015 57 M Son K F12W0 24 Sep. 2015 0 38 F L F12W4 5 Nov. 2015 4 L
F12W10 28 Dec. 2015 10 L F13W0 15 Jan. 2016 0 81 M M F13W2 16 Feb.
2016 2 M F13W13 28 Apr. 2016 13 M Contal13 27 Jan. 2016 43 F
Daughter M F14W0 21 Mar. 2016 0 65 M N F14W4 29 Apr. 2016 4 N
Contal14 22 Mar. 2016 33 M Son N F15W0 14 Sep. 2016 0 90 F O F15W2
5 Oct. 2016 2 O F15W4 18 Oct. 2016 4 O Contal15 13 Sep. 2016 52 M
Son O F16W0 26 Nov. 2015 0 83 M P F16W4 8 Jan. 2016 4 P Contal16 11
Dec. 2015 27 F Maid P S1W0 14 Jul. 2015 0 78 F S1W2 24 Jul. 2015 2
S1W4 10 Aug. 2015 4 S2W0 24 Sep. 2015 0 83 F S2W2 5 Oct. 2015 2
S2W4 19 Oct. 2015 4 S2W10 30 Nov. 2015 10 S3W0 20 Oct. 2015 0 99 F
S3W2 2 Nov. 2015 2 S3W4 16 Nov. 2015 4 S4W0 7 Jan. 2016 0 85 F S4W2
25 Jan. 2016 2 S4W10 21 Mar. 2016 10 S5W0 16 Mar. 2016 0 92 F S5W2
31 Mar. 2016 2 S5W4 19 Apr. 2016 4 S5W10 3 Jun. 2016 10 S5W13 17
Jun. 2016 13 S5W23 26 Aug. 2016 23 S6W0 6 Mar 2015 0 88 M S6W2 18
Mar. 2015 2 S6W10 5 May 2015 10 S7W0 7 May 2015 0 93 M S7W2 22 May
2015 2 S8W0 19 Jul. 2016 0 63 M S8W4 19 Aug. 2016 4 CDI25 0 86 F Q
Control12 4 Mar. 2015 Q CDI26 30 Dec. 2015 0 88 M Control17 21 Jul.
2015 55 F CDI27 31 Aug. 2015 0 66 F R Control18 26 Aug. 2015 41 M R
CDI28 7 Sep. 2015 0 84 M S Control19 8 Sep. 2015 42 M Son S CDI29 8
Oct. 2015 0 76 M CDI30 11 Dec. 2015 0 25 F T
Control20 24 Dec. 2015 33 M Brother T DI31 28 Jul. 2016 80 F CDI32
29 Aug. 2016 0 52 M Control21 Control22 Control23 Control24
TABLE-US-00004 TABLE 4 Clean. sample_num- clean_data. Dupli- data.
sample_name Aer Sequencing_platform strategy length Nreads
raw_reads clean_reads raw_data cation Mbp. F1W0 A3 Illumina_Miseq
PE300 294 0.16 179802 81730 45.46 0 24.27 F1W2 A4 Illumina_Miseq
PE300 300 0 616376 139656 22.66 0 41.9 F1W6 A5 Illumina_Miseq PE300
300 0.228 1031864 316410 30.66 0 94.92 Control1 A6 Illumina_Miseq
PE300 300 0.139 433810 181802 41.91 0 54.54 F2W0 A9 Illumina_Miseq
PE300 299 0.14 202246 158372 78.31 0 47.43 F2W2 A10 Illumina_Miseq
PE300 300 0.13 238288 185840 77.99 0 55.75 F2W4 A11 Illumina_Miseq
PE300 298 0.14 274630 230666 83.99 0 68.97 Control2 A13
Illumina_Miseq PE300 300 0.165 339154 46712 13.77 0 14.01 F3W0 A18
Illumina_Miseq PE300 300 0 231822 93316 40.25 0 27.99 F3W2 A19
Illumina_Miseq PE300 300 0.105 618984 262320 42.38 0 78.7 F3W4 A20
Illumina_Miseq PE300 300 0.13 801066 294282 36.74 0 88.28 F3W10 A21
Illumina_Miseq PE300 300 0.131 762700 277712 36.41 0 83.31 F3W17
A22 Illumina_Miseq PE300 300 0.139 696480 71662 10.29 0 21.5
Control3 A23 Illumina_Miseq PE300 300 0 207068 84600 40.86 0 25.38
F4W0 A26 Illumina_Miseq PE300 300 0 209102 67856 32.45 0 20.36 F4W2
A27 Illumina_Miseq PE300 300 0 216998 88906 40.97 0 26.67 F4W4 A28
Illumina_Miseq PE300 300 0.112 240322 93194 38.78 0 27.96 F4W5 A29
Illumina_Miseq PE300 300 0.001 132580 50098 37.79 0 15.03 F4W18 A31
Illumina_Miseq PE300 300 0 173430 49710 28.66 0 14.91 Control4 A32
Illumina_Miseq PE300 300 0.001 1708684 752020 44.01 0 225.61 F5W0
A38 Illumina_Miseq PE300 293 0.14 248084 109264 44.04 0 32.34 F5W2
A39 Illumina_Miseq PE300 297 0.14 181924 81388 44.74 0 24.25 F5W10
A40 Illumina_Miseq PE300 300 0.075 140624 54390 38.68 0 16.32 F5W18
A41 Illumina_Miseq PE300 296 0.13 192510 94050 48.85 0 27.89
Control5 A42 Illumina_Miseq PE300 300 0.208 215010 91448 42.53 0
27.43 F6W0 A43 Illumina_Miseq PE300 298 0.15 282544 219516 77.69 0
65.64 F6W2 A44 Illumina_Miseq PE300 300 0.035 298672 118338 39.62 0
35.5 F6W11 A46 Illumina_Miseq PE300 300 0.045 275570 122034 44.28 0
36.61 Control6 A47 Illumina_Miseq PE300 297 0.12 344408 244256
70.92 0 72.54 F7W0 A89 Illumina_Miseq PE300 300 0.083 257738 78176
30.33 0 23.45 F7W4 A90 Illumina_Miseq PE300 293 0.18 202056 105384
52.16 0 31.25 F7W12 A91 Illumina_Miseq PE300 300 0.068 211482 17624
8.33 0 5.29 Control7 A78 Illumina_Miseq PE300 300 0.074 321992
36754 11.41 0 11.03 F8W0 A83 Illumina_Miseq PE300 300 0 400454
105068 26.24 0 31.52 F8W20 A106 Illumina_Miseq PE300 300 0.018
329840 55682 16.88 0 16.7 Control8 A79 Illumina_Miseq PE300 300
0.037 271614 136442 50.23 0 40.93 F9W0 A127 Illumina_Miseq PE300
297 0.15 222020 181952 81.95 0 54.22 F9W2 A128 Illumina_Miseq PE300
296 0.15 303888 236462 77.81 0 69.64 F9W4 A129 Illumina_Miseq PE300
294 0.14 378688 327536 86.49 0 96.13 Control9 A130 Illumina_Miseq
PE300 300 0.055 308178 107542 34.9 0 32.26 F10W0 A50 Illumina_Miseq
PE300 299 0.15 365022 166948 45.74 0 49.5 F10W2 A51 Illumina_Miseq
PE300 298 0.13 342394 166724 48.69 0 49.6 F10W6 A52 Illumina_Miseq
PE300 294 0.14 444320 194454 43.76 0 57.07 F10W10 A53
Illumina_Miseq PE300 300 0.019 652970 251992 38.59 0 75.6 Control10
A54 Illumina_Miseq PE300 300 0.017 888528 237676 26.75 0 71.3 F11W0
A62 Illumina_Miseq PE300 293 0.15 220522 108276 49.1 0 31.89 F11W2
A63 Illumina_Miseq PE300 300 0.117 347680 112438 32.34 0 33.73
F11W4 A64 Illumina_Miseq PE300 300 0.14 265458 123800 46.64 0 36.89
Control11 A65 Illumina_Miseq PE300 300 0.076 364878 147312 40.37 0
44.19 F12W0 A66 Illumina_Miseq PE300 299 0.13 385064 168008 43.63 0
49.98 F12W4 A68 Illumina_Miseq PE300 293 0.13 239996 94998 39.58 0
28.12 F12W10 A69 Illumina_Miseq PE300 294 0.13 367404 180104 49.02
0 52.86 F13W0 A82 Illumina_Miseq PE300 300 0.015 505218 166652
32.99 0 50 F13W2 A102 Illumina_Miseq PE300 300 0.073 233864 84538
36.15 0 25.36 F13W13 A103 Illumina_Miseq PE300 297 0.14 196280
83390 42.49 0 24.64 Control13 A80 Illumina_Miseq PE300 300 0.058
306382 124604 40.67 0 37.38 F14W0 A113 Illumina_Miseq PE300 298
0.15 183402 137498 74.97 0 40.84 F14W4 A115 Illumina_Miseq PE300
297 0.12 298982 138884 46.45 0 41.11 Control14 A119 Illumina_Miseq
PE300 300 0.13 205292 159234 77.56 0 47.37 F15W0 A131
Illumina_Miseq PE300 300 0.14 629728 298766 47.44 0 88.58 F15W2
A132 Illumina_Miseq PE300 299 0.15 357030 171648 48.08 0 50.81
F15W4 A133 Illumina_Miseq PE300 300 0.15 309054 151058 48.88 0
45.09 Control15 A134 Illumina_Miseq PE300 300 0 471798 185850 39.39
0 55.76 F16W0 A7 Illumina_Miseq PE300 300 0.185 346110 73132 21.13
0 21.94 F16W4 A86 Illumina_Miseq PE300 299 0.12 327154 132150 40.39
0 39.58 Control16 A8 Illumina_Miseq PE300 300 0.147 292252 39628
13.56 0 11.89 S1W0 A33 Illumina_Miseq PE300 300 0.001 770414 328156
42.59 0 98.45 S1W2 A34 Illumina_Miseq PE300 299 0.14 175118 75890
43.34 0 22.65 S1W4 A35 Illumina_Miseq PE300 298 0.15 234894 110164
46.9 0 32.83 S2W0 A58 Illumina_Miseq PE300 297 0.14 335058 249376
74.43 0 73.94 S2W2 A59 Illumina_Miseq PE300 294 0.14 405606 276022
68.05 0 81.98 S2W4 A60 Illumina_Miseq PE300 296 0.12 176070 132478
75.24 0 39.21 S2W10 A61 Illumina_Miseq PE300 300 0.068 346262 38884
11.23 0 11.67 S3W0 A71 Illumina_Miseq PE300 300 0.108 199368 44866
22.5 0 13.46 S3W2 A72 Illumina_Miseq PE300 296 0.15 269004 119998
44.61 0 35.52 S3W4 A73 Illumina_Miseq PE300 293 0.07 291644 58196
19.95 0 17.05 S4W0 A81 Illumina_Miseq PE300 300 0.015 712372 170362
23.91 0 51.11 S4W2 A98 Illumina_Miseq PE300 299 0.15 410054 186270
45.43 0 55.32 S4W10 A100 Illumina_Miseq PE300 300 0.066 223792
94782 42.35 0 28.43 S5W0 A107 Illumina_Miseq PE300 300 0 431392
221564 51.36 0 66.47 S5W2 A108 Illumina_Miseq PE300 296 0.13 209076
72748 34.8 0 21.53 S5W4 A109 Illumina_Miseq PE300 294 0.14 245218
101786 41.51 0 30.03 S5W10 A110 Illumina_Miseq PE300 293 0.14
267352 112302 42.01 0 33.07 S5W13 A111 Illumina_Miseq PE300 293
0.14 309046 123178 39.86 0 36.21 S5W23 A112 Illumina_Miseq PE300
300 0.086 407234 89072 21.87 0 26.72 S6W0 A14 Illumina_Miseq PE300
296 0.13 300734 121426 40.38 0 36.12 S6W2 A15 Illumina_Miseq PE300
294 0.14 381812 175862 46.06 0 52.14 S6W10 A17 Illumina_Miseq PE300
293 0.09 373944 109182 29.2 0 32.26 S7W0 A24 Illumina_Miseq PE300
300 0 385622 128024 33.2 0 38.41 S7W2 A25 Illumina_Miseq PE300 297
0.12 280482 115920 41.33 0 34.54 S8W0 A120 Illumina_Miseq PE300 298
0.13 211678 100168 47.32 0 29.95 S8W4 A121 Illumina_Miseq PE300 299
0.14 219782 94604 43.04 0 28.33 CDI25 A1 Illumina_Miseq PE300 297
0.13 266926 117804 44.13 0 35.16 Control12 A2 Illumina_Miseq PE300
296 0.13 380378 163882 43.08 0 48.84 CDI26 A88 Illumina_Miseq PE300
300 0.029 539830 199604 36.98 0 59.88 Control17 A37 Illumina_Miseq
PE300 300 0.256 343310 112180 32.68 0 33.65 CDI27 A48
Illumina_Miseq PE300 300 0.12 386578 128450 33.23 0 38.53 Control18
A49 Illumina_Miseq PE300 297 0.13 353998 247752 69.99 0 73.09 CDI28
A55 Illumina_Miseq PE300 296 0.14 288796 124028 42.95 0 36.96
Control19 A57 Illumina_Miseq PE300 300 0.071 866640 91022 10.5 0
27.31 CDI29 A70 Illumina_Miseq PE300 298 0.12 331604 159998 48.25 0
47.52 CDI30 A74 Illumina_Miseq PE300 294 0.15 222838 163664 73.45 0
48.2 CDI31 A122 Illumina_Miseq PE300 300 0.071 218720 46710 21.36 0
14.01 CDI32 A124 Illumina_Miseq PE300 298 0.15 541002 285192 52.72
0 84.42 Control20 A136 Illumina_Miseq PE300 300 0.056 425346 154348
36.29 0 46.3 Control21 A137 Illumina_Miseq PE300 300 0.125 817664
347726 42.53 0 104.32 Control22 A138 Illumina_Miseq PE300 300 0.147
1584946 635954 40.12 0 190.79 Control23 A139 Illumina_Miseq PE300
300 0.155 540728 250244 46.28 0 75.07
TABLE-US-00005 TABLE 5 Sequencing_Strategy. Raw- Read_length.
sample_name sample_NUMBER Sequencing_platform PE.SE. Reads bp. F1W0
B3 Illumina_Miseq PE250 139020 250 F1W2 B4 Illumina_Miseq PE250
453216 250 F1W6 B5 Illumina_Miseq PE250 210766 250 Control1 B6
Illumina_Miseq PE250 197162 250 F2W0 B9 Illumina_Miseq PE250 145174
250 F2W2 B10 Illumina_Miseq PE250 165058 250 F2W4 B11
Illumina_Miseq PE250 157286 250 Control2 B13 Illumina_Miseq PE250
178348 250 F3W0 B18 Illumina_Miseq PE250 392454 250 F3W2 B19
Illumina_Miseq PE250 230982 250 F3W4 B20 Illumina_Miseq PE250
112378 250 F3W10 B21 Illumina_Miseq PE250 230772 250 F3W17 B22
Illumina_Miseq PE250 311224 250 Control3 B23 Illumina_Miseq PE250
523854 250 F4W0 B26 Illumina_Miseq PE250 174036 250 F4W2 B27
Illumina_Miseq PE250 220990 250 F4W4 B28 Illumina_Miseq PE250
241624 250 F4W5 B29 Illumina_Miseq PE250 180292 250 F4W18 B31
Illumina_Miseq PE250 201408 250 Control4 B32 Illumina_Miseq PE250
421468 250 F5W0 B38 Illumina_Miseq PE250 141266 250 F5W2 B39
Illumina_Miseq PE250 144132 250 F5W10 B40 Illumina_Miseq PE250
218376 250 F5W18 B41 Illumina_Miseq PE250 238492 250 Control5 B42
Illumina_Miseq PE250 350844 250 F6W0 B43 Illumina_Miseq PE250
266526 250 F6W2 B44 Illumina_Miseq PE250 236454 250 F6W11 B46
Illumina_Miseq PE250 233562 250 Control6 B47 Illumina_Miseq PE250
305446 250 F7W0 B89 Illumina_Miseq PE250 237086 250 F7W4 B90
Illumina_Miseq PE250 235976 250 F7W12 B91 Illumina_Miseq PE250
250973 250 Control7 B78 Illumina_Miseq PE250 203468 250 F8W0 B83
Illumina_Miseq PE250 131878 250 F8W20 B106 Illumina_Miseq PE250
167805 250 Control8 B79 Illumina_Miseq PE250 242486 250 F9W0 B127
Illumina_Miseq PE250 196849 250 F9W2 B128 Illumina_Miseq PE250
204701 250 F9W4 B129 Illumina_Miseq PE250 174369 250 Control9 B130
Illumina_Miseq PE250 158498 250 F10W0 B50 Illumina_Miseq PE250
283910 250 F10W2 B51 Illumina_Miseq PE250 200238 250 F10W6 B52
Illumina_Miseq PE250 170482 250 F10W10 B53 Illumina_Miseq PE250
178994 250 Control10 B54 Illumina_Miseq PE250 824186 250 F11W0 B62
Illumina_Miseq PE250 215272 250 F11W2 B63 Illumina_Miseq PE250
195352 250 F11W4 B64 Illumina_Miseq PE250 193674 250 Control11 B65
Illumina_Miseq PE250 183434 250 F12W0 B66 Illumina_Miseq PE250
130132 250 F12W4 B68 Illumina_Miseq PE250 442748 250 F12W10 B69
Illumina_Miseq PE250 376794 250 F13W0 B82 Illumina_Miseq PE250
295522 250 F13W2 B102 Illumina_Miseq PE250 163065 250 F13W13 B103
Illumina_Miseq PE250 207191 250 Control13 B80 Illumina_Miseq PE250
462154 250 F14W0 B113 Illumina_Miseq PE250 223935 250 F14W4 B115
Illumina_Miseq PE250 259086 250 Control14 B119 Illumina_Miseq PE250
247736 250 F15W0 B131 Illumina_Miseq PE250 154491 250 F15W2 B132
Illumina_Miseq PE250 148992 250 F15W4 B133 Illumina_Miseq PE250
112623 250 Control15 B134 Illumina_Miseq PE250 253469 250 F16W0 B7
Illumina_Miseq PE250 115544 250 F16W4 B86 Illumina_Miseq PE250
281377 250 Control16 B8 Illumina_Miseq PE250 192726 250 S1W0 B33
Illumina_Miseq PE250 127724 250 S1W2 B34 Illumina_Miseq PE250
156738 250 S1W4 B35 Illumina_Miseq PE250 173534 250 S2W0 B58
Illumina_Miseq PE250 223636 250 S2W2 B59 Illumina_Miseq PE250
185150 250 S2W4 B60 Illumina_Miseq PE250 159388 250 S2W10 B61
Illumina_Miseq PE250 344816 250 S3W0 B71 Illumina_Miseq PE250
241924 250 S3W2 B72 Illumina_Miseq PE250 273290 250 S3W4 B73
Illumina_Miseq PE250 332982 250 S4W0 B81 Illumina_Miseq PE250
192778 250 S4W2 B98 Illumina_Miseq PE250 198910 250 S4W10 B100
Illumina_Miseq PE250 157771 250 S5W0 B107 Illumina_Miseq PE250
239494 250 S5W2 B108 Illumina_Miseq PE250 230334 250 S5W4 B109
Illumina_Miseq PE250 264477 250 S5W10 B110 Illumina_Miseq PE250
283660 250 S5W13 B111 Illumina_Miseq PE250 281066 250 S5W23 B112
Illumina_Miseq PE250 269234 250 S6W0 B14 Illumina_Miseq PE250
144110 250 S6W2 B15 Illumina_Miseq PE250 122768 250 S6W10 B17
Illumina_Miseq PE250 177230 250 S7W0 B24 Illumina_Miseq PE250
141758 250 S7W2 B25 Illumina_Miseq PE250 135760 250 S8W0 B120
Illumina_Miseq PE250 225001 250 S8W4 B121 Illumina_Miseq PE250
231234 250 CDI25 B1 Illumina_Miseq PE250 93298 250 Control12 B2
Illumina_Miseq PE250 127534 250 CDI26 B88 Illumina_Miseq PE250
205071 250 Control17 B37 Illumina_Miseq PE250 253674 250 CDI27 B48
Illumina_Miseq PE250 168938 250 Control18 B49 Illumina_Miseq PE250
356614 250 CDI28 B55 Illumina_Miseq PE250 144894 250 Control19 B57
Illumina_Miseq PE250 474850 250 CDI29 B70 Illumina_Miseq PE250
225218 250 CDI30 B74 Illumina_Miseq PE250 232140 250 Control20 B75
Illumina_Miseq PE250 279832 250 DI31 B122 Illumina_Miseq PE250
241976 250 CDI32 B124 Illumina_Miseq PE250 166333 250 Clean_Data.
Raw_Data Read_GC Adapter_Rate Duplication_Rate N_rate sample_name .
. . Clean_Reads . . . . . . . . . . . . F1W0 36.41 50614 54.77 0 --
0 F1W2 49.63 224930 55.19 0 -- 0 F1W6 54.7 115298 54.43 0 -- 0
Control1 56.09 110582 53.81 0 -- 0 F2W0 36.12 52432 56.19 0 -- 0
F2W2 39.87 65806 53.31 0 -- 0 F2W4 51.06 80306 55.57 0 -- 0
Control2 53.95 96226 53.59 0 -- 0 F3W0 37.82 148440 51.66 0 -- 0
F3W2 44.39 102532 54.01 0 -- 0 F3W4 47.17 53006 53.36 0 -- 0 F3W10
44.67 103090 52.81 0 -- 0 F3W17 44.88 139674 54.17 0 -- 0 Control3
48.24 252724 53.82 0 -- 0 F4W0 41.06 71452 54.33 0 -- 0 F4W2 43.23
95524 55.18 0 -- 0 F4W4 36.16 87376 55.01 0 -- 0 F4W5 47.99 86526
58.15 0 -- 0 F4W18 43.77 88154 54.42 0 -- 0 Control4 49.82 209980
53.83 0 -- 0 F5W0 37.14 52462 53.82 0 -- 0 F5W2 32.13 46310 53.24 0
-- 0 F5W10 38.59 84272 54.1 0 -- 0 F5W18 36.26 86488 53.76 0 -- 0
Control5 46.31 162472 54.36 0 -- 0 F6W0 37.13 98972 54.74 0 -- 0
F6W2 44.09 104262 53.8 0 -- 0 F6W11 45.15 105462 54.06 0 -- 0
Control6 55.31 168944 54.78 0 -- 0 F7W0 79.42 188300 54.91 0 -- 0
F7W4 83.49 197021 53.01 0 -- 0 F7W12 79.33 199092 53.2 0 -- 0
Control7 50.32 102382 54.61 0 -- 0 F8W0 26.67 35168 54.94 0 -- 0
F8W20 83.11 139457 53.09 0 -- 0 Control8 58.72 142396 55.31 0 -- 0
F9W0 80.26 157992 52.39 0 -- 0 F9W2 77.69 159032 51.85 0 -- 0 F9W4
79.82 139188 53.08 0 -- 0 Control9 81.99 129950 52.62 0 -- 0 F10W0
33.77 95884 55.08 0 -- 0 F10W2 30.26 60588 55.17 0 -- 0 F10W6 38.49
65614 54.36 0 -- 0 F10W10 36.65 65606 54.59 0 -- 0 Control10 40.69
335336 53.36 0 -- 0 F11W0 42.74 92012 53.28 0 -- 0 F11W2 44.86
87634 53.31 0 -- 0 F11W4 29.48 57096 55.18 0 -- 0 Control11 47.41
86968 55.82 0 -- 0 F12W0 28.16 36646 51.56 0 -- 0 F12W4 38.11
168732 52.41 0 -- 0 F12W10 44.61 168084 54.27 0 -- 0 F13W0 35.97
106314 54.32 0 -- 0 F13W2 82.16 133971 54.28 0 -- 0 F13W13 80.73
167256 53.56 0 -- 0 Control13 48.56 224408 53.21 0 -- 0 F14W0 81.37
182212 52.37 0 -- 0 F14W4 76.58 198409 53.36 0 -- 0 Control14 78.41
194254 51.97 0 -- 0 F15W0 79.7 123134 56.75 0 -- 0 F15W2 77.43
115366 51.66 0 -- 0 F15W4 81.68 91988 55.07 0 -- 0 Control15 74.17
187995 52.93 0 -- 0 F16W0 47.42 54786 55.67 0 -- 0 F16W4 73.93
208021 51.76 0 -- 0 Control16 49.58 95562 55.47 0 -- 0 S1W0 48.49
61934 53.3 0 -- 0 S1W2 40.52 63506 53.78 0 -- 0 S1W4 38.11 66142
54.08 0 -- 0 S2W0 31.94 71434 55.8 0 -- 0 S2W2 32.01 59258 55.44 0
-- 0 S2W4 28.23 44998 55.05 0 -- 0 S2W10 39.19 135144 52.08 0 -- 0
S3W0 32.86 79506 55.38 0 -- 0 S3W2 38.59 105472 55.84 0 -- 0 S3W4
37.47 124756 53.04 0 -- 0 S4W0 45.56 87836 53.87 0 -- 0 S4W2 81.35
161811 53.93 0 -- 0 S4W10 76.87 121286 51.88 0 -- 0 S5W0 84.11
201431 55.84 0 -- 0 S5W2 82.31 189592 55.29 0 -- 0 S5W4 78.33
207172 52.18 0 -- 0 S5W10 81.24 230451 52.84 0 -- 0 S5W13 78.64
221027 55.94 0 -- 0 S5W23 76.53 206040 52.41 0 -- 0 S6W0 50.6 72918
54.66 0 -- 0 S6W2 35.01 42986 55.06 0 -- 0 S6W10 52.6 93230 53.52 0
-- 0 S7W0 41.43 58734 54.25 0 -- 0 S7W2 33.35 45270 55.72 0 -- 0
S8W0 77.52 174431 52.82 0 -- 0 S8W4 78.69 181960 52.87 0 -- 0 CDI25
29.23 27272 54.54 0 -- 0 Control12 52.3 66694 55.41 0 -- 0 CDI26
61.56 126248 53.34 0 -- 0 Control17 46.68 118412 51.69 0 -- 0 CDI27
32.75 55330 54.64 0 -- 0 Control18 48.47 172844 54.23 0 -- 0 CDI28
27.61 39998 54.42 0 -- 0 Control19 50.34 239062 55.47 0 -- 0 CDI29
28.02 63114 55.92 0 -- 0 CDI30 36.89 85644 56.9 0 -- 0 Control20
57.13 159864 54.04 0 -- 0 DI31 82.29 199112 52.56 0 -- 0 CDI32
81.76 135989 54.71 0 -- 0
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