U.S. patent application number 16/500332 was filed with the patent office on 2020-11-26 for composition containing plant extracts and the use thereof.
The applicant listed for this patent is TCI CO., LTD. Invention is credited to Ciao-Ting CHEN, I-Hui CHEN, Kai-Wen KAN, Yung-Hsiang LIN, Fu Chen LIU.
Application Number | 20200368307 16/500332 |
Document ID | / |
Family ID | 1000005016112 |
Filed Date | 2020-11-26 |
![](/patent/app/20200368307/US20200368307A1-20201126-D00000.png)
![](/patent/app/20200368307/US20200368307A1-20201126-D00001.png)
United States Patent
Application |
20200368307 |
Kind Code |
A1 |
LIN; Yung-Hsiang ; et
al. |
November 26, 2020 |
COMPOSITION CONTAINING PLANT EXTRACTS AND THE USE THEREOF
Abstract
The present invention provides a composition containing plant
extracts and the use thereof. Compared to a single plant extract,
the combination of the plant extracts of the present invention can
effectively enhance the synthesis of collagen. The plant extracts
constituting the composition include: an extract of grape seed,
black tea, green tea, red wine, citrus, spinach, green coffee bean,
blueberry, Pu-erh tea, or Four Seasons Spring tea.
Inventors: |
LIN; Yung-Hsiang; (Taipei,
TW) ; CHEN; I-Hui; (Taipei, TW) ; KAN;
Kai-Wen; (Taipei, TW) ; LIU; Fu Chen; (Taipei,
TW) ; CHEN; Ciao-Ting; (Taipei, TW) |
|
Applicant: |
Name |
City |
State |
Country |
Type |
TCI CO., LTD |
Taipei City |
|
TW |
|
|
Family ID: |
1000005016112 |
Appl. No.: |
16/500332 |
Filed: |
April 3, 2018 |
PCT Filed: |
April 3, 2018 |
PCT NO: |
PCT/CN2018/081716 |
371 Date: |
October 2, 2019 |
Related U.S. Patent Documents
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Application
Number |
Filing Date |
Patent Number |
|
|
62480860 |
Apr 3, 2017 |
|
|
|
62503185 |
May 8, 2017 |
|
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Current U.S.
Class: |
1/1 |
Current CPC
Class: |
A61K 9/08 20130101; A61K
36/21 20130101; A61K 9/48 20130101; A61K 9/0056 20130101; A61K
36/752 20130101; A61P 19/04 20180101; A61Q 19/08 20130101; A61K
36/45 20130101; A61K 36/74 20130101; A61K 36/87 20130101; A61K
36/82 20130101; A61K 8/9789 20170801 |
International
Class: |
A61K 36/82 20060101
A61K036/82; A61K 9/00 20060101 A61K009/00; A61K 36/45 20060101
A61K036/45; A61K 36/87 20060101 A61K036/87; A61K 36/21 20060101
A61K036/21; A61K 36/752 20060101 A61K036/752; A61K 36/74 20060101
A61K036/74; A61K 9/08 20060101 A61K009/08; A61K 9/48 20060101
A61K009/48; A61P 19/04 20060101 A61P019/04; A61Q 19/08 20060101
A61Q019/08; A61K 8/9789 20060101 A61K008/9789 |
Claims
1. A composition comprising a plant extract, wherein the plant
extract comprises at least one combination selected from the group
consisting of a red wine extract and a blueberry extract, a citrus
extract and a blueberry extract, a citrus extract and a Four
Seasons Spring tea extract, a spinach extract and a black tea
extract, a spinach extract and a green tea extract, a green coffee
bean extract and a blueberry extract, and a green coffee bean
extract and a Pu-erh tea extract.
2. The composition according to claim 1, wherein the plant extract
comprises at least 0.003906 mg/mL of the green coffee bean extract
and at least 0.003906 mg/mL of the blueberry extract; or at least
0.015625 mg/mL of the green coffee bean extract and at least
0.015625 mg/mL of the Pu-erh tea extract.
3. The composition according to claim 1, wherein the plant extract
comprises at least 0.125 mg/mL of the blueberry extract and at
least 0.125 mg/mL of the red wine extract.
4. The composition according to claim 1, wherein the plant extract
comprises at least 0.25 mg/mL of the spinach extract and at least
0.25 mg/mL of the green tea extract; or at least 0.5 mg/mL of the
spinach extract and at least 0.5 mg/mL of the black tea
extract.
5. The composition according to claim 1, wherein the plant extract
comprises at least 0.5 mg/mL of the citrus extract and at least 0.5
mg/mL of the Four Seasons Spring tea extract; or at least 4 mg/mL
of the citrus extract and at least 4 mg/mL of the blueberry
extract.
6. A pharmaceutical composition comprising the composition
according to claim 1 and a pharmaceutical acceptable carrier.
7. The pharmaceutical composition according to claim 6, wherein the
pharmaceutical composition is in a form of a solution, a capsule,
or a lozenge.
8. A food composition for enhancing the synthesis of collagen,
comprising the composition according to claim 1 and a food
ingredient, wherein the food ingredient is a component of a general
food, a health food, a dietary supplement, or a drink.
9. A method of enhancing the synthesis of collagen, comprising
administering to a subject in need thereof an effective amount of
the composition according to claim 1.
10. The method according to claim 9, wherein the composition is a
pharmaceutical composition.
11. The method according to claim 9, wherein the composition is a
food product.
Description
CROSS-REFERENCE TO RELATED APPLICATION
[0001] This application is a National Stage Entry of PCT
application PCT/CN2018/081716, filed on Apr. 3, 2018, claims
priority of U.S. application No. 62/480,860, filed on Apr. 3, 2017
and U.S. application No. 62/503,185, filed on May 8, 2017 the
content of which are incorporated herein in its entirety by
references.
BACKGROUND OF THE INVENTION
1. Field of the Invention
[0002] The present invention relates to a composition containing
plant extracts and the use thereof, and more particularly to the
composition containing plant extracts and the use thereof for
enhancing the synthesis of collagen.
2. The Prior Art
[0003] Collagen is the most important structural protein in animals
and the main component of extracellular matrix and connective
tissue. Collagen is widely found in tissues such as tendons, bones,
skin and cornea and is the most abundant protein in animals which
accounts for 20-30% of the total protein content and 6% of the
total body weight of the animal.
[0004] In the case of sufficient protein nutrition and vitamin C,
collagen can be synthesized by the body itself. However, the
synthesis of collagen decreases and the loss rate of collagen
increases with age, and problems of loss of collagen would happen
such as loose skin or joint wear.
[0005] Therefore, if the amount of collagen secretion in the body
can be properly increased, the skin can be kept elastic, the
formation of wrinkles can be slowed down, the strength and the
flexibility of joints can be increased, and the wear and aging of
joints can be slowed down. However, the ingredients or drugs
currently available for promoting the increase in the amount of
collagen secretion are limited. If a certain plant extract is used
to promote the increase in the amount of collagen secretion, it
must be administered at a relatively high dose, and thus there are
limitations in the cost of use and effect. Therefore, if a
composition containing plurality of plant extracts with a small
amount of each component can be administered to produce the similar
effect when administered a high-dose of a single component, the use
of the aforementioned effects can be greatly improved, and at the
same time, the use cost can be reduced.
SUMMARY OF THE INVENTION
[0006] To solve the foregoing problem, one objective of the present
invention is to provide a composition comprising a plant extract,
wherein the plant extract comprises at least one combination
selected from the group consisting of a red wine extract and a
blueberry extract, a citrus extract and a blueberry extract, a
citrus extract and a Four Seasons Spring tea extract, a spinach
extract and a black tea extract, a spinach extract and a green tea
extract, a green coffee bean extract and a blueberry extract, and a
green coffee bean extract and a Pu-erh tea extract.
[0007] In one embodiment of the present invention, the composition
comprises any of the following combinations: at least 0.003906
mg/mL of the green coffee bean extract and at least 0.003906 mg/mL
of the blueberry extract; at least 0.015625 mg/mL of the green
coffee bean extract and at least 0.015625 mg/mL of the Pu-erh tea
extract; at least 0.125 mg/mL of the blueberry extract and at least
0.125 mg/mL of the red wine extract; at least 0.25 mg/mL of the
spinach extract and at least 0.25 mg/mL of the green tea extract;
at least 0.5 mg/mL of the spinach extract and at least 0.5 mg/mL of
the black tea extract; at least 0.5 mg/mL of the citrus extract and
at least 0.5 mg/mL of the Four Seasons Spring tea extract, and at
least 4 mg/mL of the citrus extract and at least 4 mg/mL of the
blueberry extract.
[0008] The other objective of the present invention is to provide a
pharmaceutical composition for enhancing the synthesis of collagen,
comprising the aforementioned composition and a pharmaceutical
acceptable carrier.
[0009] According to an embodiment of the present invention, the
pharmaceutical composition is in a form of a solution, a capsule or
a tablet.
[0010] Another objective of the present invention is to provide a
food composition for enhancing the synthesis of collagen,
comprising the aforementioned composition and a food ingredient,
wherein the food ingredient is a component of a general food, a
health food, a dietary supplement, or a drink.
[0011] Another objective of the present invention is to provide a
method for enhancing the synthesis of collagen, comprising
administering to a subject in need thereof an effective amount of
the aforementioned composition, wherein the composition is a food
product.
[0012] The composition comprising plant extracts of the present
invention can effectively promote the synthesis of collagen,
effectively enhance the synthesis rate of collagen, enhance the
elasticity of the skin, reduce the wrinkle formation, and improve
the strength and flexibility of the joint.
BRIEF DESCRIPTION OF THE DRAWINGS
[0013] The following drawings form part of the present
specification and are included here to further demonstrate some
aspects of the present invention, which can be better understood by
reference to one or more of these drawings, in combination with the
detailed description of the embodiments presented herein.
[0014] FIG. 1 is a diagram showing the effects of different plant
extracts or combinations on the enhancement of synthesis rate of
collagen.
DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENT
[0015] The embodiments of the present invention are further
described with the following drawings. The following embodiments
are given to illustrate the present invention and are not intended
to limit the scope of the present invention, and those having
ordinary skill in the art can make some modifications and
refinements without departing from the spirit and scope of the
present invention. Therefore, the scope of the present invention is
defined by the scope of the appended claims.
Definition
[0016] As used herein, the data provided represent experimental
values that can vary within a range of .+-.20%, preferably within
.+-.10%, and most preferably within .+-.5%.
EXAMPLE 1
Preparation of Each Plant Extract
1-1 Preparation of Four Seasons Spring Tea Extract
[0017] The Four Seasons Spring tea extract was prepared as follows:
first, the leaves of Four Seasons Spring tea plant were washed and
dried, and then Four Seasons Spring tea were coarsely crushed by a
pulverizer to be a crude material. The crude material of Four
Seasons Spring tea was extracted using water as the solvent. The
weight ratio of the solvent to the crude material was 5-20:1-5. The
extraction temperature was at 50-100.degree. C., preferably
75-95.degree. C., for 0.5-3 hours to obtain the crude extract of
the Four Seasons Spring tea. After the extraction, the crude
extraction was cooled to room temperature. The crude extraction was
filtered through a 400 mesh filter to remove residual solids.
Furthermore, the filtered of the Four Seasons Spring tea was
further concentrated under reduced pressure at 45-70.degree. C. to
obtain a concentrated product and the concentrated product was the
Four Seasons Spring tea extract of the present invention.
1-2 Preparation of Green Tea Extract
[0018] The green tea extract was prepared as follows: first, the
unfermented leaves of Camellia sinensis, i.e. the green tea, were
washed and dried, and then were coarsely crushed by a pulverizer to
be a crude material. The crude material of green tea was extracted
using water as the solvent. The weight ratio of the solvent to the
crude material was 5-20:1-5. The extraction temperature was at
50-100.degree. C., preferably 75-95.degree. C., for 0.5-3 hours to
obtain the crude extract of the green tea. After the extraction,
the crude extraction was cooled to room temperature. The crude
extraction was filtered through a 400 mesh filter to remove
residual solids. Furthermore, the filtered of the green tea was
further concentrated under reduced pressure at 45-70.degree. C. to
obtain a concentrated product and the concentrated product was the
green tea extract of the present invention.
1-3 Preparation of Black Tea Extract
[0019] The black tea extract was prepared as follows: first, the
fermented leaves of Camellia sinensis, i.e. the black tea, were
washed and dried, and then were coarsely crushed by a pulverizer to
be a crude material. The crude material of black tea was extracted
using water as the solvent. The weight ratio of the solvent to the
crude material was 5-20:1-5. The extraction temperature was at
50-100.degree. C., preferably 75-95.degree. C., for 0.5-3 hours to
obtain the crude extract of the black tea. After the extraction,
the crude extraction was cooled to room temperature. The crude
extraction was filtered through a 400 mesh filter to remove
residual solids. Furthermore, the filtered of the black tea was
further concentrated under reduced pressure at 45-70.degree. C. to
obtain a concentrated product and the concentrated product was the
black tea extract of the present invention.
1-4 Preparation of Spinach Extract
[0020] The spinach extract was obtained by extracting spinach
(Spinacia oleracea), and the spinach extract used in the example
was purchased from Honh Siang Farm Products Factory.
1-5 Preparation of Red Wine Extract
[0021] The red wine extract was obtained by extracting red wine,
and the red wine extract used in the example was purchased from
Shanghai Boyoutang Biotechnology Co., Ltd.
1-6 Preparation of Citrus Extract
[0022] The citrus extract was obtained by extracting the fruits of
Citrus reticulata, and the citrus extract used in the example was
purchased from Roterm Trading Co., Ltd.
1-7 Preparation of Green Coffee Bean Extract
[0023] The green coffee bean extract was obtained by extracting the
seeds of unroasted coffee (Coffea spp.), and the green coffee bean
extract used in the example was purchased from Arjuna Natural
Extracts Ltd.
1-8 Preparation of Blueberry Extract
[0024] The blueberry extract was obtained by extracting the fruits
of Vaccinium Cyanococcus, and the blueberry extract used in the
example was purchased from BIOMED HERBAL RESEARCH CO., LTD.
1-9 Preparation of Pu-erh Tea Extract
[0025] The Pu-erh tea extract was obtained by extracting the
post-fermented leaves of Camellia sinensis, and the Pu-erh tea
extract used in the example was purchased from Nanjing Zelang
Biotechnology Co., Ltd.
1-10 Preparation of Grape Seed Extract
[0026] The grape seed extract was purchased from Guarante Biotech
Co., Ltd.
EXAMPLE 2
Effects of Combination of the Plant Extracts on Enhancing the
Synthesis of Collagen In Fibroblast Cells
[0027] The present invention analyzed the synthesis of collagen by
human skin fibroblast (CCD-966sk). The human skin fibroblast was
purchased from the Food Industry Research and Development
Institute, Taiwan, and the number is BCRC 60153. The cells were
cultured in the culture medium with 10% fetal bovine serum and 90%
minimum essential medium (MEM, purchased from Gibco, USA) and the
medium further contains 1 mM sodium pyruvate and 1% penicillin or
streptomycin.
[0028] First, 2.times.10.sup.4 of the human skin fibroblasts were
seeded in 24-well culture plates containing 500 .mu.L of the above
culture medium, and were cultured at 37.degree. C. After 24 hours
culturing, the cells were washed by phosphate buffered saline (PBS)
without disturbing the cells, and then the cells were divided into
the following 21 groups and added to 500 .mu.L of the MEM without
any fetal bovine serum: (1) 0.003906 mg/mL of the green coffee bean
extract, (2) 0.007813 mg/mL of the Four Seasons Spring tea extract,
(3) 0.5 mg/mL of the Four Seasons Spring tea extract, (4) 0.125
mg/mL of the blueberry extract, (5) 4 mg/mL of the blueberry
extract, (6) 0.125 mg/mL of the red wine extract, (7) 0.25 mg/mL of
the red wine extract, (8) 0.25 mg/mL of the spinach extract, (9)
0.5 mg/mL of the spinach extract, (10) 0.5 mg/mL of the citrus
extract, (11) 4 mg/mL of the citrus extract, (12) 0.003906 mg/mL of
the grape seed extract, (13) 0.5 mg/mL of the black tea extract,
(14) 4 mg/mL of the combination of the spinach extract and the
blueberry extract mixed in a ratio of 1:1, (15) 0.003906 mg/mL of
the combination of the green coffee bean extract and the blueberry
extract mixed in a ratio of 1:1, (16) 0.015625 mg/mL of the
combination of the green coffee bean extract and the Pu-erh tea
extract mixed in a ratio of 1:1, (17) 0.125 mg/mL of the
combination of the blueberry extract and the red wine extract mixed
in a ratio of 1:1, (18) 0.25 mg/mL of the combination of the
spinach extract and the green tea extract mixed in a ratio of 1:1,
(19) 0.5 mg/mL of the combination of the spinach extract and the
black tea extract mixed in a ratio of 1:1, (20) 0.5 mg/mL of the
combination of the Four Seasons Spring tea extract and the citrus
extract mixed in a ratio of 1:1, and (21) 4 mg/mL of the
combination of the citrus extract and the blueberry extract mixed
in a ratio of 1:1; wherein, the cells treated without any extract
was as the blank control group, and the culture medium was as the
background for the subsequent reading absorbance. After culturing
at 37.degree. C. for 48 hours, 1 mL of the culture medium of each
group was collected without disturbing the cells, and was then
placed in a 1.5 mL eppendorf. The amount of collagen secreted by
the skin fibroblasts of each group was detected by the soluble
collagen assay kit (Sircol.TM. Soluble Collagen Assay kit, Bicolor
Life Science Assays, Notherm Ireland, the UK). First, 200 .mu.L of
the collagen isolation & concentration reagent was added into
each of the eppendorf and was upside down to mix well. After
reacted at 4.degree. C. for 16-18 hours, each eppendorf was
centrifuged at 12000 rpm for 10 minutes at 4.degree. C., avoiding
shaking the tube during operation. Then, 1 mL of the supernatant
was carefully removed, and 1 mL of Sircol dye reagent was added
into each eppendorf to stain the collagen. After each eppendorf was
gently shaken for 30 minutes to mix well, it was then centrifuged
at 12000 rpm for 10 minutes at 4.degree. C. and the supernatant was
removed. Then, 750 .mu.L of Acid-salt washing reagent was added
into each eppendorf to wash away the excess dye. After centrifuged
at 12000 rpm for 10 minutes at 4.degree. C., the supernatant was
removed and the liquid remaining in the wall and lid of the
eppendorf was carefully removed with a cotton ball. Then, 250 .mu.L
of Alkali reagent was added into each eppendorf and the collagen
was uniformly dissolved by a vortex. Finally, the absorbance of the
product of the 21 groups and the blank control group at 555 nm was
measured by an enzyme linked immunosorbent assay, ELISA, analyzer.
After deducting the background, the absorbance of the differences
between the 21 groups and the blank control group was calculated,
and the student t-test was used to determine the standard deviation
and the statistically significant difference (* p<0.05; **
p<0.01; *** p<0.001).
[0029] The results of each group of the combination of the plant
extract of the present invention enhancing the synthesis rate of
collagen was shown in Table 1 and FIG. 1; wherein, the higher the
percentage, the better the ability to enhance the synthesis of
collagen. As showing in Table 1 and FIG. 1, compared with the blank
control group, the green coffee bean extract, 0.125 mg/mL of the
blueberry extract, the spinach extract, 0.5 mg/mL of the citrus
extract, and 0.5 mg/mL of the black tea extract could not
significantly enhance the synthesis of collagen, and some of them
even significantly reduced the synthesis of collagen, such as the
green coffee bean extract, 0.125 mg/mL of the blueberry extract,
0.25 mg/mL of the spinach extract, 0.5 mg/mL of the citrus extract,
and 0.5 mg/mL of the black tea extract (see the comparative
groups). However, except the combination of the spinach extract and
the blueberry extract, the synthesis rate of collagen in the groups
of the combination of the green coffee bean extract and the
blueberry extract, the combination of the green coffee bean extract
and the Pu-erh tea extract, the combination of the red wine extract
and the blueberry extract, the combination of the spinach extract
and the green tea extract, the combination of the spinach extract
and the black tea extract, the combination of the citrus extract
and the Four Seasons Spring tea extract, and the combination of the
citrus extract and the blueberry extract (see the experimental
groups) were all enhanced much more than that in each group of
single plant extract with same concentration. The result indicates
that the specific combinations described above unexpectedly exhibit
a higher ability to enhance the synthesis of collagen than a single
plant extract, and not a random combination can achieve such
effect.
TABLE-US-00001 TABLE 1 The synthesis rate of collagen of each group
Plant extract/ Concentration Synthesis rate of Group Combination
(mg/mL)/Ratio collagen (%) Control -- -- 100 Comparative green
coffee bean 0.003906 85.46 group 1 Comparative Four Seasons
0.007813 195.14 group 2 Spring tea Comparative 0.5 132.45 group 3
Comparative blueberry 0.125 78.26 group 4 Comparative 4 189.46
group 5 Comparative red wine 0.125 116 group 6 Comparative 0.25
246.29 group 7 Comparative spinach 0.25 57.82 group 8 Comparative
0.5 102.12 group 9 Comparative citrus 0.5 99.24 group 10
Comparative 4 148.92 group 11 Comparative grape seed 0.003906
186.27 group 12 Comparative black tea 0.5 59.39 group 13
Comparative spinach + 4 (1:1) 182.92 group 14 blueberry Experiment
green coffee bean + 0.003906 (1:1) 168.33 group 1 blueberry
Experiment green coffee bean + 0.015625 (1:1) 168.03 group 2 Pu-erh
tea Experiment red wine + 0.125 (1:1) 189.46 group 3 blueberry
Experiment spinach + 0.25 (1:1) 176.96 group 4 green tea Experiment
spinach + 0.5 (1:1) 161.71 group 5 black tea Experiment citrus +
0.5 (1:1) 172.85 group 6 Four Seasons Spring tea Experiment citrus
+ 4 (1:1) 210.34 group 7 blueberry
[0030] In summary, the compositions of the present invention
significantly enhance the synthesis collagen by mixing the specific
plant extracts or mixing the specific plant extracts with the
plant-derived compounds. Therefore, the composition can be utilized
to prepare pharmaceutical or food compositions that enable to
enhance the elasticity of the skin, reduce the wrinkle formation,
and improve the strength and flexibility of the joint of the
applicator or consumer.
[0031] Furthermore, the composition can be further added to a food,
a health food, a dietary supplement, or a drink. While the
composition for enhancing the synthesis of collagen of the
embodiment of the present invention is prepared to be a
pharmaceutical composition, the pharmaceutical composition can be
further added to a carrier or other adjuvant well known in the art.
The form of the pharmaceutical composition can be, but is not
limited to, a solution, a capsule, or a tablet.
* * * * *