U.S. patent application number 15/929311 was filed with the patent office on 2020-08-06 for whey preparation obtained by cavitation and uses thereof.
The applicant listed for this patent is VALIO LTD. Invention is credited to Paivi MYLLARINEN, Kirsi RAJAKARI.
Application Number | 20200245633 15/929311 |
Document ID | 20200245633 / US20200245633 |
Family ID | 1000004782281 |
Filed Date | 2020-08-06 |
Patent Application | download [pdf] |
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United States Patent
Application |
20200245633 |
Kind Code |
A1 |
RAJAKARI; Kirsi ; et
al. |
August 6, 2020 |
WHEY PREPARATION OBTAINED BY CAVITATION AND USES THEREOF
Abstract
The present invention relates to a process for
microparticulating of an ideal whey protein in a solution. The
present invention relates also to microparticulated ideal whey
protein preparation. In addition, the present invention relates to
use of the microparticulated ideal whey protein preparation in milk
based products and/or dairy products. Further, the present
invention relates to a milk based product and/or a dairy product
containing a microparticulated whey protein preparation.
Inventors: |
RAJAKARI; Kirsi; (Espoo,
FI) ; MYLLARINEN; Paivi; (Helsinki, FI) |
|
Applicant: |
Name |
City |
State |
Country |
Type |
VALIO LTD |
Helsinki |
|
FI |
|
|
Family ID: |
1000004782281 |
Appl. No.: |
15/929311 |
Filed: |
April 24, 2020 |
Related U.S. Patent Documents
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Application
Number |
Filing Date |
Patent Number |
|
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15755343 |
Feb 26, 2018 |
10667539 |
|
|
PCT/FI2016/050604 |
Aug 31, 2016 |
|
|
|
15929311 |
|
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Current U.S.
Class: |
1/1 |
Current CPC
Class: |
A23V 2300/34 20130101;
A23V 2002/00 20130101; A23V 2300/24 20130101; A23V 2300/08
20130101; A23V 2300/31 20130101; A23J 3/10 20130101; A23C 19/053
20130101; A23C 9/1307 20130101; A23J 3/08 20130101; A23V 2250/54252
20130101; A23C 21/06 20130101; A23C 9/1422 20130101; A23C 21/00
20130101 |
International
Class: |
A23C 21/00 20060101
A23C021/00; A23C 9/13 20060101 A23C009/13; A23J 3/08 20060101
A23J003/08; A23C 9/142 20060101 A23C009/142; A23C 19/05 20060101
A23C019/05; A23C 21/06 20060101 A23C021/06; A23J 3/10 20060101
A23J003/10 |
Foreign Application Data
Date |
Code |
Application Number |
Aug 31, 2015 |
FI |
20155621 |
Claims
1. A microparticulated ideal whey protein preparation, comprising
microparticulated whey proteins and .beta.-lactoglobulin polymers
having a size of >200 kDa, wherein the microparticulated ideal
whey protein preparation is a microfiltration permeate obtained
from microfiltration of a milk raw material, or a concentrated form
of the microfiltration permeate.
2. The microparticulated ideal whey protein preparation according
to claim 1, wherein the microparticulated ideal whey protein
preparation is produced by: providing an ideal whey protein
preparation, providing a cavitator, optionally preheating the ideal
whey protein preparation, treating the optionally preheated ideal
whey protein preparation with the cavitator for providing the
microparticulated ideal whey protein preparation.
3. The microparticulated ideal whey protein preparation according
to claim 1, wherein the denaturation level of .beta.-lactoglobulin
in the preparation is in the range of 90-95%.
4. The microparticulated ideal whey protein preparation according
to claim 1, wherein the viscosity of the preparation is in the
range of about 200 to 4000 mPas.
5. The microparticulated ideal whey protein preparation according
to claim 1, wherein the preparation contains microparticulated whey
proteins having a particle size of about 1-200 .mu.m.
6. The microparticulated ideal whey protein preparation according
to claim 5, wherein the preparation contains microparticulated whey
proteins having a particle size of about 1-10 .mu.m.
7. The microparticulated ideal whey protein preparation according
to claim 1, wherein the polymerization level of
.beta.-lactoglobulin in the preparation is at least 30%.
8. The microparticulated ideal whey protein preparation according
to claim 1, containing .beta.-lactoglobulin about 5.0 to about 8.0
g/100 g.
9. The microparticulated ideal whey protein preparation according
to claim 1, containing .alpha.-lactalbumin at most about 2.0 g/100
g.
10. The microparticulated ideal whey protein preparation according
to claim 1, containing .beta.-lactoglobulin, .alpha.-lactalbumin
and casein in a ratio of 0.7/0.23/0.07.
11. The microparticulated ideal whey protein preparation according
to claim 1, containing .beta.-lactoglobulin, .alpha.-lactalbumin
and casein in a ratio of 0.9/0.03/0.07.
12. The microparticulated ideal whey protein preparation according
to claim 1, wherein the preparation has a pH in the range of
6.5-7.
13. A dairy product comprising the microparticulated ideal whey
protein preparation according to claim 1.
14. The dairy product of claim 13, wherein the dairy product is an
acidified dairy product.
15. The product according to claim 13, wherein the amount of the
microparticulated ideal whey protein preparation in the dairy
product is in the range of 10 to 50% or 20 to 30% based on the
weight of the product.
Description
[0001] This application is a Divisional of U.S. application Ser.
No. 15/755,343 filed Feb. 26, 2018, which is the U.S. national
phase of International Application No. PCT/FI2016/050604 filed Aug.
31, 2016, which designated the U.S. and claims the benefit of FI
Application No. 20155621filed Aug. 31, 2015, the entire contents of
each of which are hereby incorporated by reference.
FIELD OF THE INVENTION
[0002] The present invention relates to a process for
microparticulating of ideal whey protein. The present invention
relates also to a microparticulated ideal whey protein preparation.
In addition, the present invention relates to use of the
microparticulated ideal whey protein preparation in milk based
products and/or dairy products and to a method for manufacturing
milk based and/or dairy products using a microparticulated ideal
whey protein preparation in the manufacture. Further, the present
invention relates to a milk based product and/or a dairy product
containing microparticulated ideal whey protein preparation.
BACKGROUND OF THE INVENTION
[0003] Typically microparticulated whey protein is produced from a
whey protein concentrate (WPC) solution by physically shearing the
solution during heating using a scraped-surface heat exchanger.
Commercially available products such as LeanCreme.TM. (SPX
Corporation, USA) and Simplesse (CP Kelco, USA) are used as a
heat-stable whey protein and as a fat substitute in foods, such as
cheeses, ice cream and beverages, for example. The problem related
to these traditionally produced microparticulated whey protein
products is that they do not produce a creamy texture and/or taste
into acidified milk products.
BRIEF DESCRIPTION OF THE INVENTION
[0004] The present invention relates to a process for
microparticulating of ideal whey protein. The present invention
relates also to microparticulated ideal whey protein preparation.
In addition, the present invention relates to use of the
microparticulated ideal whey protein preparation in milk based
products and/or dairy products. The present invention relates also
to a method for manufacturing milk based and/or dairy products
using a microparticulated ideal whey protein preparation in the
manufacture. Further, the present invention relates to a milk based
product and/or a dairy product containing microparticulated ideal
whey protein.
[0005] The microparticulated ideal whey protein of the present
invention contains or comprises .beta.-lactoglobulin (.beta.-LG)
polymers having a size of >200 kDa. The microparticulated ideal
whey protein of the present invention has particle size of 1-200
.mu.m. The taste of the microparticulated ideal whey protein is
creamy and full-flavored. It is well suited for use in the
manufacturing of milk based products and/or dairy products,
especially acidified milk based and/or dairy products.
[0006] The objects of the invention are achieved by
methods/products and uses characterized by what is stated in the
independent claims. The preferred embodiments of the invention are
disclosed in the dependent claims.
BRIEF DESCRIPTION OF THE DRAWINGS
[0007] FIG. 1 shows SDS-PAGE-results of different microparticulated
whey proteins:
[0008] Line MW shows the bands of Bio-Rad #161-0373 -standard.
[0009] Line 1 shows the bands of an ideal whey protein
concentrate.
[0010] Line 2 shows the bands of an ideal whey protein concentrate
microparticulated with LeanCreme.TM.-process (SPX, USA).
[0011] Line 3 shows the bands of a pasteurized ideal whey protein
concentrate microparticulated with LeanCreme.TM.-process (SPX,
USA).
[0012] Line 4 shows the bands of an ideal whey protein concentrate
microparticulated by cavitation with APV-cavitator (SPX, USA).
[0013] FIG. 2 shows SDS-PAGE-results of different microparticulated
ideal whey proteins.
[0014] Line 1 shows the bands of Bio-Rad #161-0373 -standard.
[0015] Line 2 shows the bands of an ideal whey protein.
[0016] Line 3 shows the bands of an ideal whey protein concentrate
microparticulated with APV-cavitator (SPX, USA and having .beta.-LG
denaturation degree of 79.8%.
[0017] Line 4 shows the bands of an ideal whey protein concentrate
microparticulated with APV-cavitator (SPX, USA) and having
.beta.-LG denaturation degree of 93.6%.
[0018] Lines 5 and Line 6 show the bands of an ideal whey protein
concentrate microparticulated by cavitation using APV-cavitator
(SPX, USA) and having .beta.-LG denaturation degree of 93.7%.
[0019] Line 7 shows the bands of an ideal whey protein concentrate
microparticulated by cavitation using APV-cavitator (SPX, USA) and
having .beta.-LG denaturation degree of 94.6%.
[0020] Line 8 shows the bands of an ideal whey protein concentrate
microparticulated by cavitation using APV-cavitator (SPX, USA) and
having .beta.-LG denaturation degree of 91.6%.
[0021] Line 9 shows the bands of an ideal whey protein concentrate
microparticulated by cavitation using APV-cavitator (SPX, USA) and
having .beta.-LG denaturation degree of 77.9%.
DETAILED DESCRIPTION OF THE INVENTION
[0022] The problem related to known microparticulated whey proteins
produced by homogenizing preheated whey protein concentrate (WPC)
at a high pressure and extruding then the whey proteins in acidic
conditions, is that they do not produce a creamy texture and/or
taste into milk based/dairy products, especially to acidified milk
based/dairy products.
[0023] The present invention is based on a finding that
microparticulating ideal whey protein with a cavitator produced
microparticulated ideal whey protein preparation having a creamy
and thick texture. In addition, when this microparticulated ideal
whey protein preparation was used in the manufacture of yogurt, a
creamy and thick yogurt, creamier and thicker than a reference
yogurt produced without the microparticulated ideal whey protein
product, was produced. Accordingly, it is possible to produce
low-fat or fat-free acidified milk based products/dairy products,
which have creamy taste and texture with the microparticulated
ideal whey protein preparation of the present invention. The
microparticulated ideal whey protein preparation can be used as
such and/or as a fat substitute, for example, also in neutral dairy
products as well as other foodstuffs.
[0024] In the method of the present invention, an ideal whey
protein preparation, which contains native whey proteins, was
cavitated with APV-cavitator (SPX, USA) using the following
operating values: 150-200 l/h, 50-60 Hz, cavitation time 10-60 s,
the clearance of the cavitator 3 mm (radial), holes in four (1-4)
lines. The ideal whey protein preparation was preheated to a
temperature of 25-67.degree. C. and during the cavitation the
temperature rose up to 73-93.degree. C. After this, the
microparticulated ideal whey protein was cooled to a temperature up
to 10.degree. C. using a scraped-surface heat exchanger.
[0025] As a result of the cavitation procedure, a thick/viscous
light brownish microparticulated ideal whey protein preparation
having particle size of 1-200 .mu.m, was achieved. The taste of the
microparticulated ideal whey protein preparation was creamy and
full-flavored. In addition, it was found that when the
microparticulated ideal whey protein preparation was used in
manufacturing yogurt, full-flavored, smooth and creamy yogurt with
no wheying-off or syneresis during the storage was achieved.
[0026] Accordingly, the present invention relates to a process for
microparticulating of ideal whey protein. In one embodiment, the
present invention relates to a process for microparticulating of an
ideal whey protein preparation by cavitation. The present invention
relates also to a microparticulated ideal whey protein preparation.
In one embodiment, the present invention relates to an ideal whey
protein preparation microparticulated by cavitation. In addition,
the present invention relates to use of a microparticulated ideal
whey protein preparation in a milk based product and/or a dairy
product. In one embodiment, the present invention relates to use of
an ideal whey protein preparation microparticulated by cavitation
in a milk based product and/or a dairy product. Further, the
present invention relates to a milk based product and/or a dairy
product containing microparticulated ideal whey protein. In one
embodiment, the present invention relates to a milk based product
and/or a dairy product containing an ideal whey protein preparation
microparticulated by cavitation.
[0027] The ideal whey protein preparation used in the present
invention contains whey proteins in their native form (i.e., ideal
whey proteins) and .beta.-caseins, .beta.-lactoglobulin ja
.alpha.-lactalbumin (.alpha.-La). In one embodiment, the ideal whey
protein preparation contains .beta.-lactoglobulin about 5.0 to
about 8.0 g/100 g. In one embodiment, the ideal whey protein
preparation contains .beta.-lactoglobulin about 5.0 to about 6.0
g/100 g. In one embodiment, the preparation contains
.beta.-lactoglobulin in an amount of about 5.5 g/100 g. In one
embodiment, the ideal whey protein preparation contains
.alpha.-lactalbumin less than or at most about 2.0 g/100 g. In one
embodiment, the ideal whey protein preparation contains
.alpha.-lactalbumin about 1.0 to about 2.0 g/100 g. In one
embodiment, the preparation contains .alpha.-lactalbumin in an
amount of about 1.8 g/100 g. In one embodiment, the ideal whey
preparation contains .beta.-lactoglobulin, .alpha.-lactalbumin and
casein in a ratio of 0.7/0.23/0.07 13-LG/.alpha.-La/casein. In one
embodiment, the ratio is 0.9/0.03/0.07 .beta.-LG/.alpha.-La/casein.
The casein referred herein is mainly .beta.-casein. The pH of the
ideal whey protein preparation is in the range of 6.5-7. In one
embodiment, the pH of the ideal whey protein preparation is about
6.7. The ideal whey protein preparation is obtained as a
microfiltration permeate of a milk raw material. The
microfiltration permeate can be further treated and/or concentrated
by membrane filtration (ultrafiltration, reverse osmose,
nanofiltration) and/or evaporation, for example. The term "an ideal
whey protein preparation" refers to a microfiltration (MF) permeate
obtained from microfiltration of a milk raw material. The term "an
ideal whey protein preparation" is understood to encompass also a
concentrated form of the MF permeate which is obtained, for
example, as an ultrafiltration retentate from ultrafiltration of
the MF permeate.
[0028] The ideal whey protein preparation can be produced by a
process which comprises the steps of: [0029] subjecting a milk raw
material to microfiltration to separate the ideal whey proteins as
a microfiltration permeate and a casein concentrate as a
microfiltration retentate, [0030] optionally subjecting at least a
portion of the microfiltration permeate to ultrafiltration to
provide an ultrafiltration permeate and an ideal whey protein
concentrate as an ultrafiltration retentate, [0031] optionally
evaporating at least a portion of the microfiltration permeate
and/or the ultrafiltration retentate, [0032] optionally drying at
least a portion of the microfiltration permeate and/or the
ultrafiltration retentate, and/or the evaporate, [0033] providing
the microfiltration permeate or the ultrafiltration retentate of
the microfiltration permeate or the evaporated microfiltration
permeate or the evaporated ultrafiltration retentate of the
microfiltration permeate or the dried microfiltration permeate or
the dried ultrafiltration retentate of the microfiltration permeate
as the ideal whey protein preparation.
[0034] Accordingly, in the present invention, the ideal whey
protein preparation can be in the form of a solution or in the form
of an evaporate (concentrate) or in the form of a powder. In one
embodiment, the ideal whey protein preparation is in the form of a
solution or in the form of a concentrate. In one embodiment, the
ideal whey protein preparation is in the form of a powder.
[0035] The milk raw material may be, for instance, full-fat milk,
cream, low-fat milk or skim milk, lactose-free or low-lactose milk,
protease treated milk, recombined milk from milk powder, organic
milk or a combination of these, or a dilution of any of these. Milk
can originate from a cow, sheep, goat, camel, horse or any other
animal producing milk suitable for nourishment. In one embodiment,
the milk raw material is skimmed milk. In another embodiment, the
milk raw material is low lactose or lactose-free milk. In a further
embodiment, the milk raw material is low lactose or lactose-free
skimmed milk.
[0036] In one embodiment, the ideal whey protein preparation can be
prepared by microfiltrating skimmed milk and concentrating the
obtained microfiltration permeate by ultrafiltration.
Microfiltration of the skimmed milk is typically carried out at a
temperature of about 2.degree. C. to about 55.degree. C. In one
embodiment, the microfiltration is carried out at about 10.degree.
C. Ultrafiltration is typically performed at about 5.degree. C. to
about 55.degree. C. In one embodiment, the ultrafiltration is
carried at about 10.degree. C.
[0037] The ideal whey protein preparation contains .beta.-casein,
about 20% of the total protein, but it does not contain micellar
casein monomers or any other by-products from the cheese
manufacture, i.e., it is free of caseinomacropeptides and thermally
formed -casein .beta.-lactoglobulin complexes. The protein content
of the ideal whey protein preparation can range from about 4% to
about 90%. In an embodiment, the protein content of the preparation
is about 9% and .beta.-casein content is about 20% based on total
protein. In another embodiment, the protein content of the
preparation is about 16% and .beta.-casein content is about 20%
based on total protein. The ideal whey protein preparation contains
more .alpha.-lactalbumin and .beta.-lactoglobulins in total whey
protein than whey protein concentrate (WPC) manufactured from
cheese whey because the caseinomacropeptide fraction does not exist
in the ideal whey protein preparation. The lactose content of the
ideal whey protein preparation can be reduced, if desired. The
lactose removal can be accomplished with methods known in the art,
for example. In one embodiment, the ideal whey protein preparation
is in the form of an ideal whey protein solution, which contains
about 7% whey protein, about 1.8% casein, about 2.3% lactose, about
0.47% ash, and the dry matter of the solution being about 12.2%. In
another embodiment, the ideal whey protein preparation is in the
form of an ideal whey protein powder which contains about 88% whey
protein, about 0% casein, about 0% lactose, about 3% ash, and the
dry matter of the preparation being about 90%. When ideal whey
protein in powder form is used in the preparation of the
microparticulated ideal whey protein preparation, optional steps of
adding a suitable liquid and/or dissolving the powder, known by a
skilled person, are included in the method of the present
invention. Functional properties of whey proteins are better
maintained when the drying step does not exist in the manufacturing
process of the ideal whey protein preparation. The taste of the
ideal whey protein preparation is pleasant and clean due to the
mild heat-treatment performed at temperature below 75.degree. C.,
most typically at 72.degree. C. for 15 seconds. Additionally, ideal
whey protein preparation does not carry any of the associated
off-flavors because it is not derived from cheese production.
[0038] The present invention relates to a process for producing a
microparticulated ideal whey protein preparation. In one
embodiment, the present invention relates to a process for
producing a microparticulated ideal whey protein preparation by
cavitation. In one embodiment, the method comprises the steps of:
[0039] providing an ideal whey protein preparation, [0040]
providing a cavitator, [0041] optionally preheating the ideal whey
protein preparation, treating the optionally preheated ideal whey
protein preparation in the cavitator for providing the
microparticulated ideal whey protein preparation.
[0042] In one embodiment, the ideal whey protein preparation is
preheated to the temperature of 25-67.degree. C. In one embodiment,
the ideal whey protein solution is preheated to a temperature of
48-67.degree. C. In another embodiment, the ideal whey protein
solution is preheated to a temperature of about 61-67.degree. C. In
one embodiment, the temperature at the output of the cavitator is
in the range of about 73-93.degree. C. In one embodiment, the
temperature at the output of the cavitator is in the range of about
77-93.degree. C. In another embodiment, the temperature at the
output of the cavitator is in the range of about 73-85.degree. C.
In one embodiment, the temperature at the output of the cavitator
is the range of 77-85.degree. C. In one embodiment, the ideal whey
protein solution is preheated to a temperature of about
48-67.degree. C. and the temperature at the output of the cavitator
is in the range of about 73-85.degree. C.
[0043] In one embodiment, the frequency in the cavitator is in the
range of about 50-60 Hz. In another embodiment, the frequency in
the cavitator is in the range of about 52-56 Hz.
[0044] In one embodiment, the cavitation time is about 3-60 s. In
another embodiment, the cavitation time is about 10-60 s. In one
embodiment, the cavitation time is about 10-30 s. In one
embodiment, the cavitation time is about 30-60 s. In one
embodiment, the cavitation time is about 30 s.
[0045] It was found in the present invention, that the temperature
at the output of the cavitator and the cavitation frequency, for
example, were important to the polymerization of the ideal whey
protein, especially for the polymerization of the
.beta.-lactoglobulins.
[0046] In one embodiment, the microparticulated ideal whey protein
is cooled to a temperature up to 10.degree. C. In another
embodiment, the microparticulated ideal whey protein is cooled to a
temperature of about 4.degree. C.
[0047] In one embodiment, the produced microparticulated ideal whey
protein preparation is in the form of a solution. In this
embodiment, the method for producing a microparticulated ideal whey
protein preparation further comprises an optional step step of:
[0048] concentrating the microparticulated ideal whey protein
solution to provide a microparticulated ideal whey protein
concentrate.
[0049] In one embodiment, the microparticulated ideal whey protein
preparation in a solution or a concentrate form is dried and a
microparticulated ideal whey protein in powder form is
provided.
[0050] The method of the present invention provides a
microparticulated ideal whey protein preparation, wherein the whey
proteins have a particle size of 1-200 .mu.m, 1-100 .mu.m, 1-10
.mu.m, 5-10 .mu.m or 1-2 .mu.m. In one embodiment, the whey
proteins have a particle size of 1-10 .mu.m. The microparticulated
ideal whey protein preparation contains also .beta.-caseins,
.beta.-lactoglobulin and .alpha.-lactalbumin (.alpha.-La). The
microparticulated ideal whey protein preparation contains
.beta.-casein, about 20% of the total protein. It is free of
caseinomacropeptides and thermally formed -casein
.beta.-lactoglobulin complexes. In one embodiment, the
microparticulated ideal whey protein preparation contains
.beta.-lactoglobulin about 5.0 to about 8.0 g/100 g. In one
embodiment, the ideal whey protein preparation contains
3-lactoglobulin about 5.0 to about 6.0 g/100 g. In one embodiment,
the preparation contains .beta.-lactoglobulin in an amount of about
5.5 g/100 g. In one embodiment, the microparticulated ideal whey
protein preparation contains .alpha.-lactalbumin less than or at
most about 2.0 g/100 g. In one embodiment, the ideal whey protein
preparation contains .alpha.-lactalbumin about 1.0 to about 2.0
g/100 g. In one embodiment, the preparation contains
.alpha.-lactalbumin in an amount of about 1.8 g/100 g. In one
embodiment, the microparticulated ideal whey preparation contains
.beta.-lactoglobulin, .alpha.-lactalbumin and casein in a ratio of
0.7/0.23/0.07 .beta.-LG/.alpha.-La/casein. In one embodiment, the
ratio is 0.9/0.03/0.07 .beta.-LG/.alpha.-La/casein. The casein
referred herein is mainly .beta.-casein. The pH of the
microparticulated ideal whey protein is in the range of 6.5-7. In
one embodiment, the pH of the preparation is about 6.7.
[0051] The protein content of the microparticulated ideal whey
protein preparation can range from about 4% to about 90%. In an
embodiment, the protein content of the preparation is about 9% and
.beta.-casein content is about 20% based on total protein. In
another embodiment, the protein content of the preparation is about
16% and .beta.-casein content is about 20% based on total protein.
The .beta.-lactoglobulin, or at least part of it, is in a
polymerized form in the microparticulated ideal whey protein
preparation of the present invention. The .beta.-lactoglobulin
polymers have a size of >200 kDa. This is a unique feature in a
microparticulated ideal whey protein preparation of the present
invention. In one embodiment, about 30% of .beta.-lactoglobulins is
in the polymerized form in the microparticulated ideal whey protein
preparation. In one embodiment, at least 30% of
.beta.-lactoglobulins is in the polymerized form in the
microparticulated ideal whey protein preparation. In one
embodiment, at least 33% of .beta.-lactoglobulins is in the
polymerized form in the microparticulated ideal whey protein
preparation. In one embodiment, about 50% of .beta.-lactoglobulins
is in the polymerized form in the microparticulated ideal whey
protein preparation. In one embodiment, about 99% of
.beta.-lactoglobulins is in the polymerized form in the
microparticulated ideal whey protein preparation. In one
embodiment, about 30% to about 99% of .beta.-lactoglobulins is in
the polymerized form in the microparticulated ideal whey protein
preparation. In one embodiment, about 33% to about 99% of
.beta.-lactoglobulins is in the polymerized form in the
microparticulated ideal whey protein preparation. In one
embodiment, about 30% to about 50% of .beta.-lactoglobulins is in
the polymerized form in the microparticulated ideal whey protein
preparation. In one embodiment, about 50% to about 99% of
.beta.-lactoglobulins is in the polymerized form in the
microparticulated ideal whey protein preparation.
[0052] In one embodiment, the microparticulation of the ideal whey
protein preparation is performed by cavitation. The
.beta.-lactoglobulin, or at least part of it, is in a polymerized
form in the ideal whey protein preparation microparticulated by
cavitation. The .beta.-lactoglobulin polymers have a size of
>200 kDa. This is a unique feature in a microparticulated ideal
whey protein produced by cavitation, since no polymerization has
been detected in microparticuated whey protein produced by
traditional methods. In one embodiment, about 30% of
.beta.-lactoglobulins is in the polymerized form in the
microparticulated ideal whey protein preparation produced by
cavitation. In one embodiment, at least 30% of
.beta.-lactoglobulins is in the polymerized form in the
microparticulated ideal whey protein preparation produced by
cavitation. In one embodiment, at least 33% of
.beta.-lactoglobulins is in the polymerized form in the
microparticulated ideal whey protein preparation produced by
cavitation. In one embodiment, about 50% of .beta.-lactoglobulins
is in the polymerized form in the microparticulated ideal whey
protein preparation produced by cavitation. In one embodiment,
about 99% of .beta.-lactoglobulins is in the polymerized form in
the microparticulated ideal whey protein preparation produced by
cavitation. In one embodiment, about 30% to about 99% of
.beta.-lactoglobulins is in the polymerized form in the
microparticulated ideal whey protein preparation produced by
cavitation. In one embodiment, about 33% to about 99% of
.beta.-lactoglobulins is in the polymerized form in the
microparticulated ideal whey protein preparation produced by
cavitation. In one embodiment, about 30% to about 50% of
.beta.-lactoglobulins is in the polymerized form in the
microparticulated ideal whey protein preparation produced by
cavitation. In one embodiment, about 50% to about 99% of
.beta.-lactoglobulins is in the polymerized form in the
microparticulated ideal whey protein preparation produced by
cavitation.
[0053] In the ideal whey protein, the denaturation level of
.beta.-lactoglobulin is near 0%. On the other hand, in the
microparticulated ideal whey protein preparation of the present
invention, the denaturation level of .beta.-lactoglobulin is in the
range of 90-95%. In one embodiment, the denaturation level of
.beta.-lactoglobulin is in the range of 92-94%.Thus the
polymerization level of .beta.-lactoglobulin in the ideal whey
protein preparation microparticulated by cavitation is >33%.
Without wishing to be bound to any theory, it seems that
microparticulation of ideal whey protein by cavitation produces
more fibrous form of protein compared to milk proteins in general,
which is then metabolized more slowly in the gut system and its
energy value is lower than that of normal milk protein.
[0054] The pH of the microparticulated ideal whey protein
preparation of the present invention is in the range of about
6.5-7. In one embodiment, the pH is about 6.7.
[0055] The viscosity of microparticulated ideal whey protein
preparation is in the range of about 200 to about 4000 mPas. In one
embodiment, the viscosity of microparticulated ideal whey protein
preparation is in the range of about 500 to about 2000 mPas.
[0056] The particle size of the microparticulated ideal whey
proteins in the preparation is in the range of about 1-200 .mu.m,
about 1-100 .mu.m, about 1-10 .mu.m, about 5-10 .mu.m or about 1-2
.mu.m. In one embodiment, the microparticulated ideal whey proteins
have a particle size of 1-10 .mu.m.
[0057] The ideal whey protein preparation microparticulated by
cavitation contains .beta.-lactoglobulin polymers having a size of
>200 kDa.
[0058] In addition, the present invention is directed to a use of a
microparticulated ideal whey protein preparation in the manufacture
of milk based and/or dairy product. In one embodiment, the texture
of the product is thicken by the use the microparticulated ideal
whey protein product in and/or during the manufacturing process of
the milk based and/or dairy product. In one embodiment, the
microparticulated ideal whey protein product is used in the
manufacture of an acidified dairy product. In one embodiment, the
microparticulated ideal whey protein product is added to the
acidified mass in the manufacture of an acidified dairy
product.
[0059] Further, the present invention relates to a method of
producing a milk based product/dairy product, wherein the method
comprises a step of using a microparticulated ideal whey protein
preparation in the method.
[0060] In one embodiment, the method of producing a milk based
product/dairy product comprises the steps of: [0061] providing a
milk raw material and an ideal whey protein preparation
microparticulated by cavitation, [0062] heat-treating and cooling
the milk raw material, [0063] adding the microparticulated ideal
whey protein preparation to the cooled milk raw material, [0064]
optionally treating the material in a manner characteristic to the
milk based product/dairy product, [0065] optionally packing the
product.
[0066] In one embodiment, the present invention is directed to a
method of producing a neutral, non-acidified milk based
product/dairy product, wherein the method comprises a step of using
a microparticulated ideal whey protein preparation in the
method.
[0067] In a certain embodiment, the method of producing a neutral
milk based product/dairy product comprises the steps of: [0068]
providing a milk raw material and a microparticulated ideal whey
preparation, [0069] heat-treating and cooling the milk raw
material, [0070] adding the microparticulated ideal whey protein
preparation to the cooled milk raw material, [0071] optionally
treating the material in a manner characteristic to the milk based
product/dairy product, [0072] optionally packing the product.
[0073] In one embodiment, the present invention is directed to a
method of producing an acidified milk based product/dairy product,
wherein the method comprises a step of using a microparticulated
ideal whey protein preparation in the method.
[0074] In a certain embodiment, the method of producing an
acidified milk based product/dairy product comprises the steps of:
[0075] providing a milk raw material and a microparticulated ideal
whey protein preparation, [0076] heat-treating and cooling the milk
raw material, [0077] acidifiying the cooled milk raw material,
[0078] adding the microparticulated ideal whey protein preparation
to the acidified milk raw material, [0079] optionally packing the
product.
[0080] In another embodiment, the method of producing a milk based
product/dairy product comprises the steps of: [0081] providing a
milk raw material and a microparticulated ideal whey protein
preparation, [0082] heat-treating and cooling the milk raw
material, [0083] adding the microparticulated ideal whey protein
preparation and an acidifier to the cooled milk raw material,
[0084] acidifiying the obtained mixture, [0085] mixing the
acidified mass, [0086] optionally packing the product.
[0087] In a further embodiment, the method of producing a milk
based product/dairy product comprises the steps of: [0088]
providing a milk raw material and a microparticulated ideal whey
protein preparation, [0089] mixing the milk raw material and a
microparticulated ideal whey protein preparation, [0090]
heat-treating and cooling the mixture, [0091] acidifiying the
cooled mixture, [0092] mixing the acidified mass, [0093] optionally
packing the product.
[0094] In the present invention, milk raw material may be milk
derived from an animal, such as a cow or a goat, as such or treated
in various ways. Milk may be treated by removing, for instance,
protein, fat or lactose therefrom, as a result of which
low-protein, fat-free, low-fat, lactose-free and/or low-lactose
milk is obtained. In this context, raw material milk also refers
to, for instance, pre-treated or untreated milks used in the
production of yoghurt, viili and fermented milk. In one embodiment,
the milk raw material is skimmed milk.
[0095] In the manufacture of the dairy product, the optimum
conditions for an economic, inexpensive and efficient production
process are employed. For example, conventional heat treatment
methods such as pasteurization (heating for example at about
72.degree. C. for at least 15 seconds), ESL treatment (heating for
example at about 130.degree. C. for 1 to 2 seconds), UHT treatment
(heating for example at about 138.degree. C. for 2 to 4 seconds) or
high temperature pasteurization (heating at 95.degree. C. for 5
minutes), are employed. In addition, treatments typical in the
preparation of a dairy product, such as homogenization,
acidification and enzyme treatments, for example, are performed in
a manner known in the field.
[0096] The present invention also relates to a milk based
product/dairy product containing a microparticulated ideal whey
protein preparation. In one embodiment, the present invention
relates to a milk based product/dairy product containing an ideal
whey protein preparation microparticulated by cavitation. Further,
the present invention relates to a milk based product/dairy product
produced by using said microparticulated ideal whey protein
preparation. In one embodiment, the present invention relates to a
milk based product/dairy product produced by using said ideal whey
protein preparation microparticulated by cavitation.
[0097] In the present invention, the term "dairy product" is
intended to cover all consumable milk-based products that can be
solid and/or jellied. The dairy product can be derived from, e.g.,
cow's milk, goat's milk, sheep's milk, skimmed milk, whole milk or
milk recombined from powdered milk.
[0098] The amount of the microparticulated ideal whey protein
preparation in the milk based product and/or dairy product is in
the range of 1 to 99% based on the weight of the product. In one
embodiment, the amount of the microparticulated ideal whey protein
preparation in the milk based and/or dairy product is in the range
of 10 to 50% based on the weight of the product. In another
embodiment, the amount of the microparticulated ideal whey protein
preparation in the milk based and/or dairy product is in the range
of 20 to 30% based on the weight of the product.
[0099] In one embodiment, the milk based product/dairy product is
an acidified milk based product/dairy product. In one embodiment of
the invention, the microparticulated ideal whey protein preparation
of the invention is used in the manufacture of an acidified dairy
product. The acidification conditions such as, starter culture(s),
temperature, pH and time for the production of acidified milk
products or ingredients are selected to meet the requirements of
the final product. The selection of suitable conditions belongs to
knowledge of a person skilled in the art.
[0100] The dairy product could be produced by using the
conventional acidification procedures of the dairy industry or
alternatively, the product can be soured with a chemical acidifying
agent.
[0101] In one embodiment, the neutral, non-acidified milk based
product/dairy product is selected from a pudding, a beverage/a
drink and a protein drink. In one embodiment, the acidified milk
based product/dairy product is a sour milk product and/or acidified
fresh product. In one embodiment, the acidified milk based
product/dairy product selected from yoghurt, quark, tvorog, skyr,
Greek yogurt, sour milk, kefir viili, fermented cream, Smetana,
sour cream, quark, cottage cheese and feta. In one embodiment, the
acidified milk based product/ dairy product refers to yogurt. The
viscosity of a yogurt of the present invention is in the range of
about 200 to about 4000 mPas. In one embodiment, the viscosity of a
yogurt of the present invention is about 500 to about 2000 mPas. In
one embodiment, the viscosity of a yogurt of the present invention
is about 1000 mPas. In one embodiment, the acidified milk based
product/dairy product is a drinkable product, such as a drinkable
yogurt, sour milk or kefir, for example. The viscosity of an
acidified drinkable product is in the range of about 100 to about
250 mPas, depending on the type of the product. In one embodiment,
the product is a drinkable yogurt having viscosity in the range of
about 150 to about 200 mPas. In one embodiment, the product is a
drinkable yogurt having viscosity of about 200 mPas. In one
embodiment, the drinkable product is sour milk having viscosity in
the range of about 100 to about 150 mPas. In one embodiment, the
product is sour milk having viscosity of about 120 mPas. The
texture of the product of the present invention is typically
thicker than a reference product produced without the
microparticulated ideal whey protein product.
[0102] In one embodiment, the yogurt is a set-type product, such as
a set-type yogurt. In one embodiment, the hardness of a set type
product is in the range of about 1000-about 4000 gmm. In one
embodiment, the hardness of a set type product is in the range of
about 2000-about 3000 gmm. In one embodiment, the hardness of a set
type product is about 3000 gmm.
[0103] The microparticulated ideal whey protein product of the
present invention can be used to substitute a thickening agent,
such as pectin, guar gum, xanthan gum, gum arabic and gellan gum,
for example in and/or during the manufacturing process of the milk
based and/or dairy product. In one embodiment, the
microparticulated ideal whey protein product is added to the
acidified mass in the manufacture of the acidified dairy product in
order to replace a thickener. In one embodiment, the
microparticulated ideal whey protein product is mixed with the
other starting materials before the acidifying step in the
manufacture of the acidified dairy product in order to replace a
thickening agent. A thickening agent can be substituted partly or
totally by the microparticulated ideal whey protein product of the
present invention.
[0104] It was found that the microparticulated ideal whey protein
preparation of the present invention did not cause any adverse
tastes to the products or interfere with the conventional
manufacturing processes.
[0105] The following examples illustrate the present invention. The
examples are not to be construed to limit the claims in any manner
whatsoever.
EXAMPLE 1
Production of Microparticulated Ideal Whey Protein Preparation by
Caviation
[0106] Ideal whey protein solution (protein 9%) was preheated to a
temperature of 27.degree. C. and run through an APV cavitator (SPX,
USA) using operating values 56.4 Hz and 200 l/h, lag-time 30 s. The
counterpressure of the device was 2.5 bar, wherein the device was
made to cavitate in a controlled way. The cavitator has a spinning
cylinder which has holes in four lines and a clearance of 3 mm.
During the cavitation the temperature rose up to 93.degree. C.
[0107] In the cavitator, the spinning action generates friction
internally in the liquid and the holes generate hydrodynamic
cavitation.
[0108] After this, the microparticulated ideal whey was cooled to a
temperature of 4.degree. C. using a a scraped-surface heat
exchanger.
[0109] As a result, a thick/viscous light brownish
microparticulated ideal whey protein preparation having particle
size of 1-200 m was achieved. The taste of the microparticulated
ideal whey protein preparation was full-flavored.
[0110] Based on the results of SD S-PAGE, it can be seen that part
of .beta.-lactoglobulin has polymerized into polymers having a size
of >200 kDa (FIG. 1). This a unique feature in a
microparticulated whey protein preparation produced by cavitation,
since no polymerization has been detected in microparticuated whey
protein produced by traditional methods.
EXAMPLE 2
Production of Fat-Free Yogurt (Reference Example)
[0111] The reference yogurt was prepared from skimmed milk, which
was pasteurized at 90.degree. C. for 5 minutes and then cooled to
the temperature of about 42.degree. C. The starter culture YF-L901
from Chr. Hansen (0.02%) was added and the raw material milk was
acidified until the pH was 4.5. After the desired pH was reached,
the mass was mixed and treated with YTRON rotor-stator mixer (30
Hz) and cooled to a temperature of about 20.degree. C. The product
was packed and cooled to a temperature of about 5.degree. C. in a
cold storage.
EXAMPLE 3
Production of Fat-Free Yogurt From Microparticulated Ideal Whey
Protein Concentrate
[0112] The yogurt was prepared from skimmed milk (72.2%) and the
microparticulated ideal whey protein preparation of Example 1
(27.8%). The skimmed milk was pasteurized at 90.degree. C. for 5
minutes and then cooled to the temperature of about 42.degree. C.
The starter culture YF-L901 from Chr. Hansen (0.02%) was added and
the raw material milk was acidified until the pH was 4.5. After the
desired pH was reached, the mass was mixed and treated with YTRON
rotor-stator mixer (30 Hz) and cooled to a temperature of about
20.degree. C. The microparticulated ideal whey protein preparation
was added and the product was packed and cooled to a temperature of
about 5.degree. C. in a cold storage.
[0113] The yogurt was full-flavored and creamy whereas the
reference yogurt of Example 2 had a watery, not creamy, taste.
[0114] The yogurt of the invention was about 5.3 times thicker than
the reference yogurt. After one week of storage, the viscosity of
the yogurt of the invention was 982 mPas, whereas the viscosity of
the reference yogurt produced in Example 2 was 185 mPas. The
richness/fullbodieness and smoothness of the yogurt of the
invention as well as the difference in the thickness compared to
the reference yogurt preserved during the 3-weeks storage
period.
[0115] There were no wheying-off or syneresis during the storage in
the yogurt of the invention.
EXAMPLE 4
Production of Fat-Free Drinking Yogurt From Microparticulated Ideal
Whey Protein Concentrate
[0116] The drinking yogurt was prepared from skimmed milk (60%) and
the microparticulated ideal whey protein preparation of Example 1
(40%). Whey protein preparation replaced pectin addition in the
process. The skimmed milk was pasteurized at 90.degree. C. for 5
minutes and then cooled to the temperature of about 42.degree. C.
The starter culture YO-MIX 410 from Danisco (0.02%) was added and
the raw material milk was acidified until the pH was 4.5. After the
desired pH was reached, the mass was mixed and treated with YTRON
rotor-stator mixer (30 Hz) and cooled to a temperature of about
20.degree. C. The microparticulated ideal whey protein preparation
was added and the product was packed and cooled to a temperature of
about 5.degree. C. in a cold storage.
[0117] The drinking yogurt was full-flavored and creamy whereas
normal drinking yogurt had a watery, not creamy, taste.
[0118] The drinking yogurt of the invention was more full bodied
than the reference dinking yogurt with pectin. After one week of
storage, the viscosity of the drinking yogurt of the invention was
200 mPas, whereas the viscosity of the normal drinking yogurt is
100 mPas. The richness/fullbodieness and smoothness of the drinking
yogurt of the invention as well as the difference in the thickness
compared to the reference yogurt preserved during the 3-weeks
storage period.
[0119] There were no wheying-off or syneresis during the storage in
the drinking yogurt of the invention.
EXAMPLE 5
Production of Fat-Free Sour Milk From Microparticulated Ideal Whey
Protein Concentrate
[0120] The sour milk was prepared from skimmed milk (55%) and the
microparticulated ideal whey protein preparation of Example 1
(45%). The skimmed milk was pasteurized at 90.degree. C. for 5
minutes and then cooled to the temperature of about 20.degree. C.
The mesophilic starter culture (0.02%) was added and the raw
material milk was acidified until the pH was 4.5. After the desired
pH was reached, the mass was mixed and treated with YTRON
rotor-stator mixer (30 Hz) and cooled to a temperature of about
10.degree. C. The microparticulated ideal whey protein preparation
was added and the product was packed and cooled to a temperature of
about 5.degree. C. in a cold storage.
[0121] The sour milk was full-flavored and creamy compared to the
normal sour milk.
[0122] The sour milk of the invention was about 8 times thicker
than the reference sour milk. After one week of storage, the
viscosity of the sour milk of the invention was 120 mPas, whereas
the viscosity of the normal sour milk is about 10-15 mPas. The
richness/fullbodieness and smoothness of the sour milk of the
invention as well as the difference in the thickness compared to
the normal sour milk preserved during the 2-weeks storage
period.
[0123] There were no wheying-off or syneresis during the storage in
the sour milk of the invention.
EXAMPLE 6
Production of Fat-Free Yogurt From Microparticulated Ideal Whey
Protein Concentrate
[0124] The yogurt was prepared from skimmed milk (72.2%) and the
microparticulated ideal whey protein preparation of Example 1
(27.8%). The skimmed milk and the microparticulated ideal whey
protein preparation were mixed and pasteurized at 90.degree. C. for
5 minutes and then cooled to the temperature of about 42.degree. C.
The starter culture YF-L901 from Chr. Hansen (0.02%) was added and
the raw material was acidified until the pH was 4.5. After the
desired pH was reached, the mass was mixed and treated with
YTRON-mixer (30 Hz) and cooled to a temperature of about 20.degree.
C. The product was packed and cooled to a temperature of about
5.degree. C. in a cold storage.
[0125] The yogurt of the invention was creamy, whereas the
reference yogurt of Example 2 had a watery, not creamy, taste. The
yogurt of the invention was thicker than the reference yogurt of
Example 2. The richness of the yogurt of the invention as well as
the difference in the thickness compared to the reference yogurt
preserved during the 3-weeks storage period.
EXAMPLE 7
Production of Fat-Free Yogurt From Microparticulated Ideal Whey
Protein Concentrate
[0126] The yogurt was prepared from skimmed milk (72.2%) and the
microparticulated ideal whey protein preparation of Example 1
(27.8%).
[0127] The skimmed milk was pasteurized at 90.degree. C. for 5
minutes and then cooled to the temperature of about 42.degree. C.
The microparticulated ideal whey protein preparation and the
starter culture YF-L901 from Chr. Hansen (0.02%) were added and the
raw material was acidified until the pH was 4.5. After the desired
pH was reached, the mass was mixed and treated with YTRON
rotor-stator mixer (30 Hz) and cooled to a temperature of about
20.degree. C. The product was packed and cooled to a temperature of
about 5.degree. C. in a cold storage.
[0128] The yogurt was full-flavored and creamy whereas the
reference yogurt of Example 2 had a watery, not creamy, taste.
[0129] The yogurt of the invention was thicker than the reference
yogurt. After one week of storage, the viscosity of the yogurt of
the invention was 982 mPas, whereas the viscosity of the reference
yogurt was 185 mPas. The richness and smoothness of the yogurt of
the invention as well as the difference in the thickness compared
to the reference yogurt preserved during the 3-weeks storage
period.
EXAMPLE 8
Production of Microparticulated Ideal Whey Protein Preparation by
Cavitation
[0130] Ideal whey protein solution (protein 9%) was preheated to a
temperature of 61.degree. C. and run through an APV cavitator (SPX,
USA) using operating values 52 Hz and 200 l/h, cavitation-time 30
s. The counterpressure of the device was 8 bar, wherein the device
was made to cavitate in a controlled way. The cavitator has a
spinning cylinder which has holes in four lines and a clearance of
3 mm. During the cavitation the temperature rose up to 78.degree.
C.
[0131] In the cavitator, the spinning action generates friction
internally in the liquid and the holes generate hydrodynamic
cavitation.
[0132] After this, the microparticulated ideal whey was cooled to a
temperature of 4.degree. C. using a scraped-surface heat
exchanger.
[0133] As a result, a thick/viscous light brownish
microparticulated ideal whey protein preparation having particle
size of 1-200 .mu.m was achieved. The taste of the
microparticulated ideal whey protein preparation was full-flavored.
Texture was smooth.
[0134] Based on the results of SD S-PAGE, it can be seen that a
part of .beta.-lactoglobulin has polymerized into polymers having a
size of >200 kDa (FIG. 2, line 8). Furthermore, the denaturation
degree of .beta.-lactoglobulin was 91.6%.
EXAMPLE 9
Production of Fat-Free Set-Type Yogurt From Microparticulated Ideal
Whey Protein Concentrate
[0135] The yogurt was prepared from skimmed milk (72.2%) and the
microparticulated ideal whey protein preparation of Example 1
(27.8%). The skimmed milk and the microparticulated ideal whey
protein preparation were mixed and pasteurized at 90.degree. C. for
5 minutes and then cooled to the temperature of about 42.degree. C.
The starter culture YF-L901 from Chr. Hansen (0.02%) was added and
the raw material was packed to cups. The product was acidified at
42.degree. C. until the pH was 4.5. After the desired pH was
reached, the product was cooled to a temperature of about 5.degree.
C.
[0136] The yogurt of the invention was creamy and the hardness of
the set-type yogurt was 3000 gmm. The richness of the yogurt of the
invention as well as the hardness of the yogurt preserved during
the 3-weeks storage period.
EXAMPLE 10
Preparation of Ideal Whey Protein Preparation
[0137] Skim milk was microfiltered with polymeric microfiltration
membranes of 800 kDa (Synder FR-3A-6338) at 10.degree. C. The
obtained microfiltration permeate was concentrated by
ultrafiltration with an ultrafiltration membrane of 10 kDa (Koch
HFK-131 6438-VYT) and with a concentration factor of 36 at
10.degree. C. to provide an ideal whey protein solution/preparation
as an ultrafiltration retentate. The protein content of the ideal
whey solution was 9%. .beta.-casein content is about 20% based on
total protein.
[0138] It will be obvious to a person skilled in the art that, as
the technology advances, the inventive concept can be implemented
in various ways. The invention and its embodiments are not limited
to the examples described above but may vary within the scope of
the claims.
* * * * *