U.S. patent application number 16/689552 was filed with the patent office on 2020-04-02 for tomato variety nun 09225 tof.
The applicant listed for this patent is NUNHEMS B.V.. Invention is credited to Ben SILVERTAND.
Application Number | 20200100450 16/689552 |
Document ID | / |
Family ID | 69947563 |
Filed Date | 2020-04-02 |
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United States Patent
Application |
20200100450 |
Kind Code |
A1 |
SILVERTAND; Ben |
April 2, 2020 |
TOMATO VARIETY NUN 09225 TOF
Abstract
The disclosure provides a new and distinct hybrid tomato variety
NUN 09225 TOF as well as seeds and plants and fruits thereof. NUN
09225 TOF is a large, round intense tomato variety for the fresh
market, comprising resistance to Verticillium dahliae, Fusarium
oxysporum f.sp. lycopersici (Fol) Race 0, Race 1, and Race 2,
Fusarium oxysporum f.sp. radicis lycopersici (Forl), Fulvia fulva
Group E, Tomato Mosaic Virus (ToMV) Strains 0, 1, and 2, and Oidium
neolypersici.
Inventors: |
SILVERTAND; Ben; (Nunhem,
NL) |
|
Applicant: |
Name |
City |
State |
Country |
Type |
NUNHEMS B.V. |
Nunhem |
|
NL |
|
|
Family ID: |
69947563 |
Appl. No.: |
16/689552 |
Filed: |
November 20, 2019 |
Related U.S. Patent Documents
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Application
Number |
Filing Date |
Patent Number |
|
|
62770314 |
Nov 21, 2018 |
|
|
|
Current U.S.
Class: |
1/1 |
Current CPC
Class: |
A01H 5/10 20130101; A01H
5/08 20130101; A01H 6/825 20180501 |
International
Class: |
A01H 6/82 20060101
A01H006/82; A01H 5/10 20060101 A01H005/10 |
Claims
1. A plant, plant part, or seed of tomato variety NUN 09225 TOF,
wherein a representative sample of seed of said variety is
deposited under Accession Number NCIMB ______.
2. The plant part of claim 1, wherein said plant part is a fruit, a
leaf, pollen, an ovule, a cell, a scion, a root, a rootstock, a
cutting, or a flower.
3. A seed that produces the plant of claim 1.
4. A plant produced by growing the seed of claim 1.
5. A tomato plant or a part thereof having all of the physiological
and morphological characteristics of the plant of claim 1.
6. A tomato plant or a part thereof which does not differ from the
plant of claim 1 in any of the characteristics listed in Tables 1
and 2 when grown under the same environmental conditions, and
wherein a representative sample of seed of tomato variety NUN 09225
TOF is deposited under Accession Number NCIMB ______.
7. A tissue or cell culture comprising regenerable cells of the
plant or plant part of claim 1.
8. The tissue or cell culture according to claim 7, comprising
cells or protoplasts from a plant part suitable for vegetative
reproduction, wherein the plant part is a meristem, a cotyledon, a
hypocotyl, pollen, a leaf, an anther, a root, a root tip, a pistil,
a petiole, a flower, a fruit, a stem, or a stalk.
9. A tomato plant regenerated from the tissue or cell culture of
claim 7, wherein the plant has all of the physiological and
morphological characteristics of the plant of variety NUN 09225
TOF, when grown under the same environmental conditions, and
wherein a representative sample of seed of said tomato variety is
deposited under Accession Number NCIMB ______.
10. A method of producing the plant of claim 1 or a part thereof,
said method comprising vegetative propagating at least a part of
the plant of variety NUN 09225 TOF, wherein a representative sample
of seed of said variety is deposited under Accession Number NCIMB
______.
11. The method of claim 10, wherein said vegetative propagating
comprises regenerating a whole plant from a part of the plant of
variety NUN 09225 TOF, wherein a representative sample of seed of
said tomato variety is deposited under Accession Number NCIMB
______.
12. The method of claim 10, wherein said part is a cutting, a cell
culture, or a tissue culture.
13. A vegetative propagated plant of claim 1, or a part thereof,
wherein the vegetative propagated plant and part thereof have all
of the physiological and morphological characteristics of tomato
variety NUN 09225 TOF, when grown under the same environmental
conditions, and wherein a representative sample of seed of said
tomato variety is deposited under Accession Number NCIMB
______.
14. A method of producing a tomato plant, said method comprising
crossing the plant of claim 1 with a second tomato plant one or
more times, selecting a progeny tomato plant from said crossing and
optionally allowing the progeny tomato plant to form seed.
15. A method of producing a tomato plant, said method comprising
selfing a plant of claim 1 one or more times, and selecting a
progeny tomato plant from said selfing and optionally allowing the
progeny tomato plant to form seed.
16. A method of introducing a desired trait into the plant of claim
1, said method comprising transforming the plant of claim 1 with a
transgene that confers the desired trait, wherein the desired trait
yield, storage properties, color, flavor, size, firmness, fruit
quality, enhanced nutritional quality, post-harvest quality, male
sterility, herbicide tolerance, insect resistance, pest resistance,
disease resistance, environmental stress tolerance, modified
carbohydrate metabolism, modified protein metabolism, or
ripening.
17. A tomato plant produced by the method of claim 16, wherein the
transformed plant otherwise retains all of the physiological and
morphological characteristics of tomato variety NUN 09225 TOF and
contains the desired trait.
18. A method of producing doubled haploid cells of the plant of
claim 1, said method comprising making double haploid cells from
haploid cells from the plant or plant part of tomato variety NUN
09225 TOF, wherein a representative sample of seed of said tomato
variety is deposited under Accession Number NCIMB ______.
19. A plant comprising the scion or rootstock of claim 2.
20. A container comprising the plant, plant part, or seed of claim
1.
21. A method of producing a tomato fruit, said method comprising
growing the plant of claim 1 until it sets at least one fruit, and
collecting the fruit.
22. A fruit produced by the method of claim 21, wherein the fruit
has all of the physiological and morphological characteristics of
tomato variety NUN 09225 TOF, a representative sample of seed of
tomato is deposited under Accession Number NCIMB ______.
23. A method of producing a modified tomato plant having a single
trait, said method comprises mutating the tomato plant or plant
part of variety NUN 09225 TOF, wherein a representative sample of
seed of said tomato variety is deposited under Accession Number
______, and wherein the modified plant has otherwise all of the
physiological and morphological characteristics of tomato variety
NUN 09225 TOF, and the single trait, wherein the trait is yield,
storage properties, color, flavor, size, firmness, fruit quality,
enhanced nutritional quality, post-harvest quality, male sterility,
herbicide tolerance, pest resistance, environmental stress
resistance, modified carbohydrate metabolism, modified protein
metabolism, or ripening or the mutation occurs in any of the
following genes acs2, acs4, rin, pp2c1, arf9, intense, myb12.
24. A method of determining the genotype of the plant of claim 1,
said method comprising obtaining a sample of nucleic acids from
said plant and detecting in said nucleic acids a plurality of
polymorphisms, thereby determining the genotype of the plant and
storing the results of detecting the plurality of polymorphisms on
a computer readable medium.
25. A method of producing a tomato plant, said method crossing
tomato plants and harvesting the resultant seed, wherein at least
one tomato plant is the plant of claim 1, wherein a representative
sample of seed of tomato variety NUN 09225 TOF is deposited under
Accession Number NCIMB ______.
26. The tomato seed produced by the method of claim 25.
27. The tomato plant or part thereof produced by growing the seed
of claim 26.
28. A method of introducing a single locus conversion into the
plant of claim 1, comprising: a. crossing the plant of claim 1 with
a second tomato plant comprising a desired single locus to produce
F1 progeny plants; b. selecting F1 progeny plants that have the
single locus to produce selected F1 progeny plants; c. crossing
selected F1 progeny plants with tomato variety NUN 09225 TOF to
produce backcross progeny plants; d. selecting backcross progeny
plants that have the single locus conversion and otherwise comprise
all of the physiological and morphological characteristics of the
tomato variety NUN 09225 TOF to produce selected backcross progeny
plants; and e. repeating steps (c) and (d) one or more times in
succession to produce selected second or higher backcross progeny
plants that comprise the single locus and otherwise comprise all of
the physiological and morphological characteristics of the tomato
variety NUN 09225 TOF, wherein a representative sample of seed of
said tomato variety has been deposited under Accession Number NCIMB
______.
29. The method of claim 28, wherein the single locus confers yield,
storage properties, color, flavor, size, firmness, fruit quality,
enhanced nutritional quality, post-harvest quality, male sterility,
herbicide tolerance, insect resistance, pest resistance, disease
resistance, environmental stress tolerance, modified carbohydrate
metabolism, modified protein metabolism, or ripening.
30. A tomato plant produced by the method of claim 28, wherein the
plant otherwise has all of the physiological and morphological
characteristics of the plant of tomato variety NUN 09225 TOF,
further comprising the single locus conversion.
31. A method of producing a tomato plant derived from the plant of
claim 1, comprising: a. preparing a progeny tomato plant derived
from tomato variety NUN 09225 TOF by crossing the plant of claim 1
with itself or with a second tomato plant; b. crossing the progeny
plant with itself or a second tomato plant to produce seed of a
progeny plant of a subsequent generation; c. growing a progeny
plant of a subsequent generation from said seed and crossing the
progeny plant of a subsequent generation with itself or a second
tomato plant; and d. repeating steps (b) and/or (c) for at least
one more generation to produce a tomato plant derived from tomato
variety NUN 09225 TOF.
Description
CROSS REFERENCE TO RELATED APPLICATION
[0001] This application claims priority to U.S. Provisional
Application No. 62/770,314, filed Nov. 21, 2018, which is hereby
incorporated by reference in its entirety.
FIELD OF THE DISCLOSURE
[0002] The disclosure relates to the field of plant breeding and,
more specifically, to the tomato variety NUN 09225 TOF. The
disclosure further relates to vegetative reproductions of tomato
variety NUN 09225 TOF, methods for tissue culture of tomato variety
NUN 09225 TOF, methods for regenerating a plant from such a tissue
culture, and to phenotypic variants of tomato variety NUN 09225
TOF. The disclosure also relates to progeny of tomato variety NUN
09225 TOF and the hybrid varieties obtained by crossing tomato
variety NUN 09225 TOF as a parent line with plants of other
varieties or parent lines.
BACKGROUND
[0003] The goal of plant breeding is to combine various desirable
traits in a single variety. Such desirable traits may include
greater yield, resistance to diseases, insects or other pests,
tolerance to heat and drought, better agronomic quality, higher
nutritional value, enhanced growth rate and improved fruit
properties.
[0004] Breeding techniques take advantage of a plant's method of
pollination. There are two general methods of pollination:
self-pollination and cross-pollination. A plant self-pollinates if
pollen from one flower is transferred to the same or another flower
of the same genotype. A plant cross-pollinates if pollen comes to
it from a flower of a different genotype.
[0005] Plants that have been self-pollinated and selected for
(uniform) type over many generations become homozygous at almost
all gene loci and produce a uniform population of true breeding
progeny of homozygous plants. A cross between two such homozygous
plants of different lines produces a uniform population of hybrid
plants that are heterozygous for many gene loci. The extent of
heterozygosity in the hybrid is a function of the genetic distance
between the parents. Conversely, a cross of two plants each
heterozygous at a number of loci produces a segregating population
of hybrid plants that differ genetically and are not uniform. The
resulting non-uniformity makes performance unpredictable.
[0006] The development of uniform varieties requires the
development of homozygous inbred plants, the crossing of these
inbred plants to make hybrids, and the evaluation of the hybrids
resulting from the crosses. Pedigree breeding and recurrent
selection are examples of breeding methods that have been used to
develop inbred plants from breeding populations. Those breeding
methods combine the genetic backgrounds from two or more plants or
various other broad-based sources into breeding pools from which
new lines are developed by selfing and selection of desired
phenotypes. The new plants are evaluated to determine which have
commercial potential.
[0007] Tomato (Solanum lycopersicum and closely related species) is
naturally a diploid and the basic chromosome number of the genus is
x=12, most are 2n=2x=24, including the cultivated ones. It
originated in the New World and has since become a major food
crop.
[0008] Tomato cultivars may be grouped by maturity, i.e., the time
required from planting the seed to the stage where fruit harvest
can occur. Standard maturity classifications include `early`,
`midseason` or `late-maturing.` Another classification for tomatoes
is the developmental timing of fruit set. `Determinate` plants grow
foliage, then transition into a reproductive phase of flower
setting, pollination and fruit development. Consequently,
determinant cultivars have a large proportion of the fruit ripen
within a short time frame. Growers that harvest only once in a
season favor determinant type cultivars. In contrast,
`indeterminate` types grow foliage, then enter a long phase where
flower and fruit development proceed along with new foliar growth.
Growers that harvest the same plants multiple times favor
indeterminate type cultivars.
[0009] Tomatoes can also be classified by their target markets:
fresh market and processing tomatoes. Fresh-market tomatoes are
primarily used for salads, salad bar and sandwiches, and require
good storage properties. On the other hand, processing tomatoes
generally requires red colored and pink to red/crimson fruit flesh
and higher percentage of soluble solids. Processing tomatoes can be
canned whole, canned, diced or chopped, dried, roasted, pasted,
pureed or concentrated, juiced, frozen, or put into ready-made
dishes, for example, sauces, stews or soups.
[0010] In 2017, World Atlas (available at world-wide web word atlas
under
articles/which-are-the-world-s-leading-tomato-producing-countries)
reported that the worldwide production of tomatoes amounted to
170.8 million tons. United States is ranked as the third largest
producer of tomatoes in the world, next to China and India.
Tomatoes are available in the United States year-round, with
California and Florida being the major producers. Fresh-market
tomatoes are available from May to December although supply peaks
in July and in September through October. Processing tomatoes have
the greatest supply from August to September.
[0011] In response to more recent consumer demands for dietary
diversity, tomato breeders have developed a wider range of colors.
In addition to expanding the range of red colored fruits, there are
cultivars that produce fruits that are creamy white, lime green,
yellow, green, golden, orange and purple. Additionally, there are
multi-colored varieties exemplified by mainly red fruited varieties
with green shoulders, and both striped- and variegated-colored
fruit.
SUMMARY OF VARIOUS ASPECTS OF THE DISCLOSURE
[0012] The disclosure provides for tomato variety NUN 09225 TOF,
products thereof, and methods of using the same. NUN 09225 TOF is a
medium, round intense tomato variety for the fresh market and is
suitable for growing in a protected (greenhouse) environment.
[0013] In one aspect, the disclosure provides a seed of tomato
variety NUN 09225 TOF, wherein a representative sample of said seed
has been deposited under Accession Number NCIMB ______. The
disclosure also provides for a plurality of seeds of tomato variety
NUN 09225 TOF. The seed of tomato variety NUN 09225 TOF may be
provided as an essentially homogeneous population of tomato seed.
The population of seed of tomato variety NUN 09225 TOF may be
particularly defined as being essentially free from other seed. The
seed population may be grown into plants to provide an essentially
homogeneous population of tomato plants as described herein.
[0014] The disclosure also provides a plant grown from a seed of
tomato variety NUN 09225 TOF and plant part thereof.
[0015] The disclosure furthermore provides a tomato fruit produced
on a plant grown from a seed of variety NUN 09225 TOF.
[0016] In another aspect, the disclosure provides a seed growing or
grown on a plant of variety NUN 09225 TOF (i.e., produced after
pollination of the flower of tomato variety NUN 09225 TOF).
[0017] In other aspects, the disclosure provides for a plant part
obtained from variety NUN 09225 TOF, wherein said plant part is: a
fruit, a harvested fruit, a part of a fruit, a leaf, a part of a
leaf, pollen, an ovule, a cell, a petiole, a shoot or a part
thereof, a stem or a part thereof, a root or a part thereof, a root
tip, a cutting, a seed, a part of a seed, seed coat or another
maternal tissue which is part of a seed grown on said variety, a
hypocotyl, a cotyledon, a scion, a stock, a rootstock, a pistil, an
anther, or a flower or a part thereof. Fruits are particularly
important plant parts. Such plant parts may be suitable for sexual
reproduction, vegetative reproduction, or a tissue culture. In
another aspect, the plant part obtained from variety NUN 09225 TOF
is a cell, optionally a cell in a cell or tissue culture. That cell
may be grown into a plant of variety NUN 09225 TOF.
[0018] In another aspect, the disclosure provides for an inbred
variety of NUN 09225 TOF.
[0019] In another aspect, the disclosure provides for a hybrid
tomato variety NUN 09225 TOF. The disclosure also provides for a
progeny of tomato variety NUN 09225 TOF. In another aspect, the
disclosure provides a plant or a progeny retaining all or all but
one, two or three of the "distinguishing characteristics" or all or
all but one, two, or three of the "morphological and physiological
characteristics" of tomato variety NUN 09225 TOF and methods for
producing that plant or progeny.
[0020] In one aspect, the disclosure provides a plant or a progeny
having all the physiological and morphological characteristics of
variety NUN 09225 TOF when grown under the same environmental
conditions. In another aspect, the plant or progeny has all or all
but one, two, or three of the physiological and morphological
characteristics of tomato variety NUN 09225 TOF when measured under
the same environmental conditions and e.g., evaluated at
significance levels of 1%, 5% or 10% significance (which can also
be expressed as a p-value) for quantitative characteristics,
wherein a representative sample of seed of variety NUN 09225 TOF
has been deposited under Accession Number NCIMB ______. In another
aspect, the plant or progeny has all or all but one, two, or three
of the physiological and morphological characteristics as listed in
Tables 1 and 2 for variety NUN 09225 TOF when measured under the
same environmental conditions and e.g., evaluated at significance
levels of 1%, 5% or 10% significance (which can also be expressed
as a p-value) for quantitative characteristics.
[0021] In another aspect, a plant of variety NUN 09225 TOF or a
progeny thereof has 13, 14, or more or all of the following
distinguishing characteristics when compared to the Reference
Variety as shown in Table 3: 1) horizontal leaf attitude; 2)
smaller size of leaflets; 3) darker intensity of green color of
mature leaf; 4) shorter peduncle length of mature leaf; 5) no green
shoulder; 6) oblate mature fruit shape at longitudinal section; 7)
stronger ribbing at peduncle end; 8) stronger depression at
peduncle end; 9) flat shape at blossom end; 10) larger size of core
in cross-section; 11) thicker pericarp; 12) glossier skin; 13)
colorless epidermis; and 14) medium shell life, when grown under
the same environmental conditions.
[0022] In another aspect, the plant of variety NUN 09225 TOF, or
part thereof, or a progeny thereof comprises resistance to
Verticillium dahliae, Fusarium oxysporum f.sp. lycopersici (Fol)
Race 0, Race 1, and Race 2, Fusarium oxysporum f.sp. radicis
lycopersici (Forl), Fulvia fulva Group E, Tomato Mosaic Virus
(ToMV) Strains 0, 1, and 2, and Oidium neolypersici, measured
according to UPOV standards described in TG/44/11.
[0023] The disclosure also provides a cell culture of tomato
variety NUN 09225 TOF and a plant regenerated from tomato variety
NUN 09225 TOF, which plant has all the characteristics of variety
NUN 09225 TOF when grown under the same environmental conditions,
as well as methods for culturing and regenerating tomato variety
NUN 09225 TOF. Alternatively, a regenerated plant may have one
characteristic that is different from tomato variety NUN 09225
TOF.
[0024] The disclosure further provides a vegetatively propagated
plant of variety NUN 09225 TOF having all or all but one, two or
three of the morphological and physiological characteristics of
tomato variety NUN 09225 TOF when grown under the same
environmental conditions as well as methods for vegetatively
propagating tomato variety NUN 09225 TOF.
[0025] In another aspect, the disclosure provides a method of
producing a tomato plant comprising crossing a first parent tomato
plant with a second parent tomato plant and harvesting the
resultant hybrid tomato seed, wherein said first parent plant or
second parent tomato plant is tomato variety NUN 09225 TOF. Also
provided is a hybrid tomato seed produced from crossing a first
parent tomato plant and second parent tomato plant and harvesting
the resultant hybrid tomato seed, wherein said first parent plant
or second parent tomato plant is tomato variety NUN 09225 TOF.
Moreover, the hybrid tomato plant grown from the hybrid tomato seed
is provided.
[0026] In another aspect, the disclosure provides a method of
introducing a single locus conversion into a plant of variety NUN
09225 TOF, wherein a representative sample of seed of tomato
variety has been deposited under Accession Number NCIMB ______,
wherein the plant otherwise retains all of the physiological and
morphological characteristics of tomato variety NUN 09225 TOF and
further comprises the single locus conversion.
[0027] In yet another aspect, the disclosure provides a method for
introducing a desired trait into tomato variety NUN 09225 TOF, said
method comprises transforming the plant of variety NUN 09225 TOF
with a transgene that confers the desired trait, wherein the
transformed plant otherwise has all of the physiological and
morphological characteristics of tomato variety NUN 09225 TOF and
contains the desired trait.
[0028] The disclosure also provides a method of producing a
modified tomato plant with a desired trait, wherein the method
comprises mutating the plant or plant part of tomato variety NUN
09225 TOF, wherein a representative sample of seed of said tomato
variety has been deposited under Accession Number NCIMB ______, and
wherein the mutated plant otherwise retains all of the
physiological and morphological characteristics of tomato variety
NUN 09225 TOF and contains the desired trait.
[0029] In one aspect, the single locus conversion or desired trait
is yield, storage properties, color, flavor, size, firmness, fruit
quality, enhanced nutritional quality, post-harvest quality, male
sterility, herbicide tolerance, insect resistance, pest resistance,
disease resistance, environmental stress tolerance, modified
carbohydrate metabolism, modified protein metabolism, or
ripening.
[0030] In another aspect, the disclosure provides a container
comprising the plant, plant part, or seed of tomato variety NUN
09225 TOF.
[0031] Also provided is a food, a feed, or a processed product
comprising a plant part of tomato variety NUN 09225 TOF, wherein
the plant part is a fruit or part thereof.
BRIEF DESCRIPTION OF DRAWINGS
[0032] FIG. 1 shows the mature leaves of tomato variety NUN 09225
TOF.
[0033] FIG. 2 shows the mature fruits of tomato variety NUN 09225
TOF.
[0034] FIG. 3 shows the cross-section of the mature fruit of tomato
variety NUN 09225 TOF.
[0035] FIG. 4 shows the plant of tomato variety NUN 09085 TOF.
[0036] FIG. 5 shows the mature fruits of tomato variety NUN 09085
TOF.
[0037] FIG. 6 shows the cross-section of the mature fruit of tomato
variety NUN 09085 TOF.
DEFINITIONS
[0038] "Tomato" refers herein to plants of the species Solanum
lycopersicum, or a closely related species, and fruits thereof.
Solanum lycopersicum is also known as Lycopersicon lycopersicum
(L.) H. Karst. or Lycopersicon esculentum Mill. The most commonly
eaten part of a tomato is the fruit or berry.
[0039] "Cultivated tomato" refers to plants of Solanum
lycopersicum, or a closely related species (e.g., varieties,
breeding lines or cultivars of the species S. lycopersicum as well
as crossbreds thereof, or crossbreds with other Solanum species),
cultivated by humans and having good agronomic characteristics.
[0040] The terms "tomato plant designated NUN 09225 TOF," "NUN
09225 TOF," "NUN 09225," "NUN 09225 F1," "09225 TOF," or "tomato
09225," are used interchangeably herein and refer to a tomato plant
of variety NUN 09225 TOF, representative seed of which is deposited
under Accession Number NCIMB ______.
[0041] "Plant" includes the whole plant or any part or derivatives
thereof, preferably having the same genetic makeup as the plant
from which it is obtained.
[0042] "Plant part" includes any part of a plant, such as a plant
organ (e.g., harvested or non-harvested fruits), a plant cell, a
plant protoplast, a plant cell tissue culture or a tissue culture
from which a whole plant can be regenerated, a plant cell that is
intact in a plant, a clone, a micropropagation, plant callus, a
plant cell clump, a plant transplant, a vegetative propagation, a
seedling, a fruit, a harvested fruit, a part of a fruit, a leaf, a
part of a leaf, pollen, an ovule, an embryo, a petiole, a shoot or
a part thereof, a stem or a part thereof, a root or a part thereof,
a root tip, a cutting, a seed, a part of a seed, hypocotyl,
cotyledon, a scion, a graft, a stock, a rootstock, a pistil, an
anther, and a flower or parts of any of these and the like. Seed
can be mature or immature. Pollen or ovules may be viable or
non-viable. Also, any developmental stage is included, such as
seedlings, cuttings prior or after rooting, mature plants or
leaves. Alternatively, a plant part may also include a plant seed
which comprises one or two sets of chromosomes derived from the
parent plant, e.g., from tomato variety NUN 09225 TOF. An F1
progeny produced from self-pollination of tomato variety NUN 09225
TOF will thus comprise two sets of chromosomes derived from tomato
variety NUN 09225 TOF, while an F1 progeny derived from
cross-fertilization of tomato variety NUN 09225 TOF will comprise
only one set of chromosomes from tomato variety NUN 09225 TOF, and
the other set of chromosomes from the other parent.
[0043] A "seed of NUN 09225 TOF" refers to a tomato seed which can
be grown into a plant of NUN 09225 TOF, wherein a representative
sample of viable seed of NUN 09225 TOF is deposited under Accession
Number NCIMB ______. A seed can be in any stage of maturity, for
example, a mature, viable seed, or an immature, non-viable seed. A
seed comprises an embryo and maternal tissues.
[0044] An "embryo of NUN 09225 TOF" refers to an "F1 hybrid embryo"
as present in a seed of NUN 09225 TOF, a representative sample of
said seed of NUN 09225 TOF is deposited under Accession Number
NCIMB ______.
[0045] A "seed grown on NUN 09225 TOF" refers to a seed grown on a
mature plant of variety NUN 09225 TOF or inside a fruit of tomato
variety NUN 09225 TOF. The "seed grown on NUN 09225 TOF" contains
tissues and DNA of the maternal parent, tomato variety NUN 09225
TOF. The "seed grown on NUN 09225 TOF" contains an F2 embryo. When
said seed is planted, it grows into a first generation progeny
plant of variety NUN 09225 TOF.
[0046] A "fruit of NUN 09225 TOF" refers to a fruit containing
maternal tissues of tomato variety NUN 09225 TOF as deposited under
Accession Number NCIMB ______. The fruit comprises pericarp, septa,
epidermis, columella, locular cavity, vascular bundles and
optionally seed. Pericarp, septa, epidermis, columella, locular
cavity, vascular bundles, and seed coat of the seed are maternal
tissues, e.g., they are genetically identical to the plant on which
they grow. In one aspect, the fruit contains seed grown on tomato
variety NUN 09225 TOF. In another aspect, the fruit does not
contain seed, i.e., the fruit is parthenocarpic. The skilled person
is familiar with methods for inducing parthenocarpy. Those methods
comprise chemically or genetically inducing parthenocarpy.
Compounds suitable for chemically inducing parthenocarpy comprise
auxins, gibberellins and cytokinins. Methods for genetically
inducing parthenocarpy comprise the methods described in U.S. Pat.
No. 9,125,353, US 2002/0010953, U.S. Pat. No. 6,060,648, EP 1057401
and EP 1428425, which are herein incorporated by reference in their
entireties.
[0047] An "essentially homogeneous population of tomato seed" is a
population of seeds where at least 97%, 98%, 99% or more of the
total population of seed are seed of tomato variety NUN 09225
TOF.
[0048] An "essentially homogeneous population of tomato plants" is
a population of plants where at least 97%, 98%, 99% or more of the
total population of plants are plants of variety NUN 09225 TOF.
[0049] The phrase "essentially free from other seed" refers to a
population of seed where less than 3%, 2%, 1% or less of the total
population of seed is seed that is not a tomato seed or, in another
aspect, less than 3%, 2%, 1% or less of the total population of
seed is seed that is not seed of tomato variety NUN 09225 TOF.
[0050] "Harvest maturity" is referred to as the stage at which a
tomato fruit is ripe or ready for harvest or the optimal time to
harvest the fruit for the market, for processing or for
consumption. In one aspect, harvest maturity is the stage which
allows proper completion of the normal ripening.
[0051] "Harvested plant material" refers herein to plant parts
(e.g., single fruits or clusters of fruits detached from the whole
plant), which have been collected for further storage and/or
further use.
[0052] "Yield" means the total weight of all tomato fruits
harvested per hectare of a particular line or variety. It is
understood that "yield" expressed as weight of all tomato fruits
harvested per hectare can be obtained by multiplying the number of
plants per hectare times the "yield per plant".
[0053] "Marketable yield" means the total weight of all marketable
tomato fruits, especially fruit which is not cracked, damaged or
diseased, harvested per hectare of a particular line or variety. A
"marketable fruit" is a fruit that has commercial value.
[0054] "USDA descriptors" are the plant variety descriptors
described for tomatoing the "Objective Description of Variety
Tomato (Solanum lycopersicum or Lycopersicon esculentum Mill)", as
published by the US Department of Agriculture, Agricultural
Marketing Service, Plant Variety Protection Office, Beltsville, Md.
20705 and which can be downloaded from the world wide web at
ams.usda.gov under services/plant-variety-protection/pvpo-c-forms
under tomato. "Non-USDA descriptors" are other descriptors suitable
for describing tomato.
[0055] "UPOV descriptors" are the plant variety descriptors
described for tomato in the "Guidelines for the Conduct of Tests
for Distinctness, Uniformity and Stability, TG/44/11 (Geneva 2011,
revised 2018 Oct. 30), as published by UPOV (International Union
for the Protection of New Varieties and Plants), and which can be
downloaded from the world wide web at upov.int/under
edocs/tgdocs/en/tg044.pdf and is herein incorporated by reference
in its entirety. Likewise, "UPOV methods" to determine specific
parameters for the characterization of tomato are described at
upov.int.
[0056] "Calibration book for Tomato (Lycopersicon esculentum
Mill.)" refers to the calibration book for tomato which provides
guidance for describing a tomato variety, as published by
Naktuinbow (version 1, December 2010). The calibration book is
based on the CPVO (Community Plant Variety Protection Office)
Protocol CPVO-TP/044/3 and UPOV TG/44/10.
[0057] "RHS" refers to the Royal Horticultural Society (RHS) which
publishes an official botanical color chart quantitatively
identifying colors according to a defined numbering system. The
chart may be purchased from Royal Horticulture Society Enterprise
Ltd RHS Garden; Wisley, Woking; Surrey GU236QB, UK, e.g., the RHS
color chart 2007.
[0058] "Reference Variety for NUN 09225 TOF" refers herein to
variety NUN 09085 TOF, a variety from Nunhems B. V., which has been
planted in a trial together with NUN 09225 TOF. The characteristics
of tomato variety NUN 09225 TOF are compared with the
characteristics of the Reference Variety as shown in Table 1. The
disease resistances of tomato variety NUN 09225 TOF and the
Reference Variety are shown in Table 2. The distinguishing
characteristics between tomato variety NUN 09225 TOF and the
Reference Variety are shown in Table 3.
[0059] A plant having "all the physiological and morphological
characteristics" of a referred-to-plant means a plant showing the
physiological and morphological characteristics of the
referred-to-plant when grown under the same environmental
conditions, preferably in the same experiment; the
referred-to-plant can be a plant from which it was derived, e.g.,
the progenitor plant, the parent, the recurrent parent, the plant
used for tissue- or cell culture, etc. A physiological or
morphological characteristic can be a numerical characteristic or a
non-numerical characteristic. In one aspect, a plant has "all but
one, two or three of the physiological and morphological
characteristics" of a referred-to-plant, or "all the physiological
and morphological characteristics" of Tables 1 and 2 or "all or all
but one, two or three of the physiological and morphological
characteristics" of Tables 1 and 2.
[0060] The physiological and/or morphological characteristics
mentioned above are commonly evaluated at significance levels of
1%, 5% or 10% if they are numerical (quantitative), or for having
an identical degree (or type) if not numerical (not quantitative),
if measured under the same environmental conditions. For example, a
progeny plant or a Single Locus Converted plant or a mutated plant
of variety NUN 09225 TOF may have one or more (or all) of the
essential physiological and/or morphological characteristics of
said variety listed in Tables 1 and 2, as determined at the 5%
significance level (i.e., p<0.05), when grown under the same
environmental conditions.
[0061] "Distinguishing characteristics" or "distinguishing
morphological and/or physiological characteristics" refers herein
to the characteristics which distinguish (i.e., are different)
between the new variety and other tomato varieties, such as the
Reference Variety, when grown under the same environmental
conditions. The distinguishing characteristics between tomato
variety NUN 09225 TOF and Reference Variety are described in Table
3. When comparing tomato variety NUN 09225 TOF with different
varieties, the distinguishing characteristics will be different. In
one aspect, the distinguishing characteristics may therefore
include at least one, two, three or more (or all) of the
characteristics listed in Tables 1 and 2. All numerical
distinguishing characteristics are statistically significantly
different at p<0.05 between tomato variety NUN 09225 TOF and the
other variety (e.g., Reference Variety).
[0062] Tomato variety NUN 09225 TOF has the following
distinguishing characteristics when compared to the Reference
Variety as shown in Table 3: 1 horizontal leaf attitude; 2) smaller
size of leaflets; 3) darker intensity of green color of mature
leaf; 4) shorter peduncle length of mature leaf; 5) no green
shoulder; 6) oblate mature fruit shape at longitudinal section; 7)
stronger ribbing at peduncle end; 8) stronger depression at
peduncle end; 9) flat shape at blossom end; 10) larger size of core
in cross-section; 11) thicker pericarp; 12) glossier skin; 13)
colorless epidermis; and 14) medium shell life, when grown under
the same environmental conditions.
[0063] Thus, a tomato plant "comprising the distinguishing
characteristics of NUN 09225 TOF" (such as a progeny plant) refers
herein to a plant which does not differ significantly from said
variety in the distinguishing characteristics above. Therefore, in
one aspect, the disclosure provides a plant that does not differ
significantly from tomato variety NUN 09225 TOF in the
distinguishing characteristics above.
[0064] Similarity and differences between two different plant lines
or varieties can be determined by comparing the number of
morphological and/or physiological characteristics (e.g., the
characteristics as listed in Tables 1 and 2) that are the same
(i.e., statistically not significantly different) or that are
different (i.e., statistically significantly different) between the
two plant lines or varieties when grown under the same
environmental conditions. A numerical characteristic is considered
to be "the same" when the value for a numeric characteristic is not
significantly different at the 1% (p<0.01) or 5% (p<0.05)
significance level, using one way analysis of variance (ANOVA), a
standard method known to the skilled person. Non-numerical or
"degree" or "type" characteristic is considered "the same" when the
values have the same "degree" or "type" when scored using USDA
and/or UPOV descriptors, if the plants are grown under the same
environmental conditions.
[0065] "Variety" or "cultivar" means a plant grouping within a
single botanical taxon of the lowest rank.
[0066] A "plant line" is, for example, a breeding line which can be
used to develop one or more varieties. A breeding line is typically
highly homozygous.
[0067] "Harvested seeds" refer to seeds harvested from a line or
variety, e.g., produced after self-fertilization or
cross-fertilization and collected.
[0068] "Hybrid variety" or "F1 hybrid" refers to the seeds
harvested from crossing two inbred (nearly homozygous) parental
lines. For example, the female parent is pollinated with pollen of
the male parent to produce hybrid (F1) seeds on the female
parent.
[0069] "Locus" (plural loci) refers to the specific location, place
or site of a DNA sequence on a chromosome, where, for example, a
gene or genetic marker is found. A locus may confer a specific
trait.
[0070] "Allele" refers to one or more alternative forms of a gene
locus. All of these loci relate to one trait. Sometimes, different
alleles can result in different observable phenotypic traits, such
as different pigmentation. However, many variations at the genetic
level result in little or no observable variation. If a
multicellular organism has two sets of chromosomes, i.e., diploid,
these chromosomes are referred to as homologous chromosomes, i.e.,
diploid. Diploid organisms have one copy of each gene (and
therefore one allele) on each chromosome. If both alleles are the
same, they are homozygotes. If the alleles are different, they are
heterozygotes.
[0071] "Genotype" refers to the genetic composition of a cell or
organism.
[0072] "Phenotype" refers to the detectable characteristic of a
plant, cell, or organism, which characteristics are the
manifestation of gene expression.
[0073] "Haploid" refers to a cell or organism having one set of the
two sets of chromosomes in a diploid.
[0074] "Diploid" refers to a plant, a vegetative plant part(s), or
seed which a diploid plant can be grown, having two sets of
chromosomes, designated herein as 2n.
[0075] "Triploid" refers to a plant, a vegetative plant part(s), or
seed which a diploid plant can be grown, having three sets of
chromosomes, designated herein as 3n.
[0076] "Tetraploid" refers to a plant, a vegetative plant part(s),
or seed which a diploid plant can be grown, having four sets of
chromosomes, designated herein as 4n.
[0077] "Scion" refers to a part of the plant attached to the
rootstock. This plant is selected for its stems, leaves, flowers,
or fruits. The scion contains the desired genes to be duplicated in
future production by the stock/scion plant and may produce the
desired tomato fruit.
[0078] "Rootstock" or "stock" refers to the plant selected for its
roots, in particular for the resistance of the roots to diseases or
stress (e.g., heat, cold, salinity etc.). Normally the quality of
the fruit of the plant providing the rootstock is less
important.
[0079] "Stock/scion" or grafted plant refers to a tomato plant
comprising a rootstock from one plant grafted to a scion from
another plant.
[0080] "Grafting" refers to the method of joining of (genetically)
different plant parts, especially scions and rootstocks, together
so that they grow as a single plant. A grafted seedling or a
grafted plant is a seedling or plant (produced by grafting)
consisting of such different plant parts and which grows as one
plant.
[0081] The term "traditional breeding techniques" encompasses
herein crossing, selfing, selection, doubled haploid production,
embryo rescue, protoplast fusion, marker assisted selection,
mutation breeding, etc. as known to the breeder (e.g., methods
other than genetic modification/transformation/transgenic methods),
by which, for example, a genetically heritable trait can be
transferred from one tomato line or variety to another.
[0082] "Tissue culture" or "cell culture" refers to a composition
comprising isolated cells of the same or a different type or a
collection of such cells organized into parts of a plant. Tissue
culture of various tissues of tomato and regeneration of plants
therefrom is well known and widely published (see, e.g., Bhatia et
al. (2004), Plant Cell, Tissue and Organ Culture 78: 1-21).
Similarly, methods of preparing cell cultures are known in the
art.
[0083] "Regeneration" refers to the development of a plant from
cell culture or tissue culture or vegetative propagation.
[0084] "Vegetative propagation," "vegetative reproduction," or
"clonal propagation" are used interchangeably herein and mean a
method of taking a part of a plant and allowing that plant part to
form at least roots, and also refer to the plant or plantlet
obtained by that method. Optionally, the vegetative propagation is
grown into a mature plant. The skilled person is aware of what
plant parts are suitable for use in the method.
[0085] "Selfing" refers to self-pollination of a plant, i.e., the
transfer of pollen from the anther to the stigma of the same
plant.
[0086] "Crossing" refers to the mating of two parent plants. The
term encompasses "cross-pollination" and "selfing".
[0087] "Cross-pollination" refers to the fertilization by the union
of two gametes from different plants.
[0088] "Backcrossing" is a traditional breeding technique used to
introduce a trait into a plant line or variety. The plant
containing the trait is called the donor plant and the plant into
which the trait is transferred is called the recurrent parent. An
initial cross is made between the donor parent and the recurrent
parent to produce a progeny plant. Progeny plants which have the
trait are then crossed to the recurrent parent. After several
generations of backcrossing and/or selfing the recurrent parent
comprises the trait of the donor. The plant generated in this way
may be referred to as a "single trait converted plant". The
technique can also be used on a parental line of a hybrid.
[0089] "Progeny" as used herein refers to a plant obtained from a
plant designated NUN 09225 TOF. A progeny may be obtained by
regeneration of cell culture or tissue culture or parts of a plant
of said variety or selfing of a plant of said variety or by
producing seeds of a plant of said variety. In further aspects,
progeny may also encompass plants obtained from crossing of at
least one plant of said variety with another tomato plant of the
same variety or another variety or (breeding) line, or with wild
tomato plants. A progeny may comprise a mutation or a transgene. A
"first generation progeny" or is the progeny directly derived from,
obtained from, obtainable from or derivable from the parent plant
by, e.g., traditional breeding methods (selfing and/or
cross-pollinating) or regeneration (optimally combined with
transformation or mutation). Thus, a plant of variety NUN 09225 TOF
is the male parent, the female parent or both of a first generation
progeny of that variety. Progeny may have all the physiological and
morphological characteristics of variety NUN 09225 TOF when grown
under the same environmental conditions. Using common breeding
methods such as backcrossing or recurrent selection, mutation or
transformation, one or more specific characteristics may be
introduced into said variety, to provide a plant comprising all but
1, 2, or 3 or more of the morphological and physiological
characteristics of variety NUN 09225 TOF (as listed in Tables 1 and
2).
[0090] The terms "gene converted" or "conversion plant" or "single
locus converted plant" in this context refer to tomato plants which
are developed by traditional breeding techniques e.g.,
backcrossing, or via genetic engineering or through mutation
breeding, wherein essentially all of the desired morphological and
physiological characteristics of the parent variety or line are
recovered, in addition to the one or more genes transferred into
the parent via e.g., the backcrossing technique (optionally
including reverse breeding or reverse synthesis of breeding lines).
It is understood that not only the addition of a further
characteristic (e.g., addition of gene conferring a further
characteristic, such as a disease resistance gene), but also the
replacement/modification of an existing characteristic by a
different characteristic is encompassed herein (e.g., mutant allele
of a gene can modify the phenotype of a characteristic).
[0091] Likewise, a "Single Locus Converted (Conversion) Plant"
refers to plants developed by plant breeding techniques comprising
or consisting of mutation and/or by genetic transformation and/or
by traditional breeding techniques, such as backcrossing, wherein
essentially all of the desired morphological and physiological
characteristics of a tomato variety are recovered in addition to
the characteristics of the single locus having been transferred
into the variety via the above mentioned technique. In case of a
hybrid, the gene may be introduced in the male or female parental
line.
[0092] "Transgene" or "chimeric gene" refers to a genetic locus
comprising a DNA sequence which has been introduced into the genome
of the plant by transformation. A plant comprising a transgene
stably integrated into its genome is referred to as "transgenic
plant."
[0093] As used herein, the terms "resistance" and "tolerance" are
used interchangeably to describe plants that show no symptoms or
significantly reduced symptoms to a specified biotic pest,
pathogen, abiotic influence or environmental condition compared to
a susceptible plant. These terms are optionally also used to
describe plants showing some symptoms but that are still able to
produce marketable product with an acceptable yield.
[0094] "Average" refers herein to the arithmetic mean.
[0095] The term "mean" refers to the arithmetic mean of several
measurements. The skilled person understands that the appearance of
a plant depends to some extent on the growing conditions of said
plant. The mean, if not indicated otherwise within this
application, refers to the arithmetic mean of measurements on at
least 10 different, randomly selected plants of a variety or
line.
DETAILED DESCRIPTION OF VARIOUS ASPECTS OF THE DISCLOSURE
[0096] The disclosure also relates to a plant of variety NUN 09225
TOF, wherein a representative sample of seeds of said variety has
been deposited under the Budapest Treaty, with Accession number
NCIMB ______. NUN 09225 TOF is a round, large intense tomato
variety for the fresh market and is suitable for growing in a
protected (greenhouse) environment.
[0097] The disclosure further relates to a tomato variety NUN 09225
TOF, which when compared to the Reference Variety has the following
distinguishing characteristics as shown in Table 3: 1) horizontal
leaf attitude; 2) smaller size of leaflets; 3) darker intensity of
green color of mature leaf; 4) shorter peduncle length of mature
leaf; 5) no green shoulder; 6) oblate mature fruit shape at
longitudinal section; 7) stronger ribbing at peduncle end; 8)
stronger depression at peduncle end; 9) flat shape at blossom end;
10) larger size of core in cross-section; 11) thicker pericarp; 12)
glossier skin; 13) colorless epidermis; and 14) medium shell life,
when grown under the same environmental conditions. Also
encompassed are parts of that plant.
[0098] In one aspect, the plant of variety NUN 09225 TOF or a
progeny plant thereof, comprises all of the following morphological
and/or physiological characteristics (i.e., as indicated on the
UPOV Test Guidelines for tomato) as shown in Tables 1 and 2, when
grown under the same environmental conditions. A part of this plant
is also provided.
[0099] In another aspect, the plant of tomato variety NUN 09225
TOF, or part thereof, or a progeny thereof comprises resistance to
Verticillium dahliae, Fusarium oxysporum f.sp. lycopersici (Fol)
Race 0, Race 1, and Race 2, Fusarium oxysporum f.sp. radicis
lycopersici (Forl), Fulvia fulva Group E, Tomato Mosaic Virus
(ToMV) Strains 0, 1, and 2, and Oidium neolypersici, measured
according to UPOV standards described in TG/44/11.
[0100] The disclosure further provides a tomato plant which does
not differ from the physiological and morphological characteristics
of the plant of variety NUN 09225 TOF as determined at the 1%, 2%,
3%, 4% or 5% significance level when grown under the same
environmental conditions. In a particular aspect, the plants are
measured in the same trial (e.g., the trial is conducted as
recommended by USDA or UPOV). The disclosure also comprises a part
of said plant, preferably a fruit or part thereof.
[0101] The morphological and/or physiological differences between
two different individual plants described herein (e.g., between
tomato variety NUN 09225 TOF and a progeny of said variety) or
between a plant of variety NUN 09225 TOF or progeny of said
variety, or a plant having all, or all but 1, 2, or 3 of the
physiological and morphological characteristics of tomato variety
NUN 09225 TOF (or all, or all but 1, 2, or 3 of the characteristics
as listed in Tables 1 and 2) and another known variety can easily
be established by growing said variety next to each other or next
to the other variety (in the same field, under the same
environmental conditions), preferably in several locations which
are suitable for said tomato cultivation, and measuring
morphological and/or physiological characteristics of a number of
plants (e.g., to calculate an average value and to determine the
variation range/uniformity within the variety). For example, trials
can be carried out in Acampo Calif., USA (N 38 degrees 07'261''/W
121 degrees 18'807'', USA), whereby various characteristics, for
example, maturity, days from seeding to harvest, plant habit, plant
attitude, leaf shape, leaf color, blistering, numbers of flowers
per leaf axil, number of calyx lobes, number of petals, fruit
group, immature fruit color, mature fruit color, pungency, flavor,
fruit glossiness, fruit size, fruit shape, average number of fruits
per plant, seed size, seed weight, anthocyanin level, disease
resistance, insect resistance, can be measured and directly
compared for species of tomato.
[0102] Thus, the disclosure comprises tomato plant having one, two
or three physiological and/or morphological characteristics which
are different from those of the plant of variety NUN 09225 TOF and
which otherwise has all the physiological and morphological
characteristics of the plant of variety NUN 09225 TOF, when
determined at the 5% significance level for plants grown under the
same environmental conditions. In one aspect, the different
characteristic(s) is/are result of breeding with tomato variety NUN
09225 TOF and selection of a progeny plant comprising 1, 2 or 3
characteristics which are different than in tomato variety NUN
09225 TOF. In another aspect, the different characteristic is the
result of a mutation (e.g., spontaneous mutation or a human induced
mutation through e.g., targeted mutagenesis or traditional
mutagenesis such as chemically or radiation induced mutagenesis),
or it is the result of transformation.
[0103] The disclosure also relates to a seed of tomato variety NUN
09225 TOF, wherein a representative sample of said seed has been
deposited under the Budapest Treaty, with Accession number NCIMB
______.
[0104] In another aspect, a seed of hybrid variety NUN 09225 TOF is
obtainable by crossing the male parent of said variety with the
female parent of said variety and harvesting the seeds produced on
the female parent. The resultant seeds of said variety can be grown
to produce plants of said variety. In one aspect, a seed or a
plurality of seeds of said variety are packaged into a container of
any size or type (e.g., bags, cartons, cans, etc.). The seed may be
disinfected, primed and/or treated with various compounds, such as
seed coatings or crop protection compounds. The seed produces a
plant of variety NUN 09225 TOF.
[0105] In another aspect, the disclosure provides a plant grown
from a seed of tomato variety NUN 09225 TOF and plant part
thereof.
[0106] The disclosure also provides a tomato fruit produced on a
plant grown from a seed of tomato variety NUN 09225 TOF.
[0107] In another aspect, the disclosure provides for a tomato
plant part of variety NUN 09225 TOF, preferably a fruit or part
thereof, a representative sample of seed from said variety is
deposited under the Budapest Treaty, with Accession number NCIMB
______.
[0108] Also provided is a plant of tomato variety NUN 09225 TOF, or
a fruit or other plant part thereof, produced from a seed, wherein
a representative sample of said seeds is deposited under the
Budapest Treaty, with Accession Number NCIMB ______.
[0109] Also provided is a plant part obtained from variety NUN
09225 TOF, wherein said plant part is a fruit, a harvested fruit, a
part of a fruit, a leaf, a part of a leaf, pollen, an ovule, a
cell, a petiole, a shoot or a part thereof, a stem or a part
thereof, a root or a part thereof, a root tip, a cutting, a seed, a
part of a seed, seed coat or another maternal tissue which is part
of a seed grown on said variety, a hypocotyl, a cotyledon, a scion,
a stock, a rootstock, a pistil, an anther, and a flower or a part
thereof. Such plant parts may be suitable for sexual reproduction
(e.g., a pollen, a flower, an ovary, an ovule, an embryo, etc.),
vegetative reproduction (e.g., a cutting, a root, a stem, a cell, a
protoplast, a leaf, a cotyledon, a meristem, etc.) or tissue
culture (e.g., a leaf, a pollen, an embryo, a cotyledon, a
hypocotyl, a cell, a root, a root tip, an anther, a flower, a seed,
a stem, etc.). Fruits are particularly important plant parts.
Fruits may be parthenocarpic, or seedless, or contain immature
and/or nonviable seeds. In a further aspect, the plant part
obtained from variety NUN 09225 TOF is a cell, optionally a cell in
a cell or tissue culture. That cell may be grown into a plant of
variety NUN 09225 TOF. A part of the plant of variety NUN 09225 TOF
(or of progeny of that variety or of a plant having all
physiological and/or morphological characteristics but one, two or
three which are different from those of tomato variety NUN 09225
TOF) further encompasses any cells, tissues, or organs obtainable
from the seedlings or plants in any stage of maturity.
[0110] The disclosure also provides a tissue or cell culture
comprising cells of tomato variety NUN 09225 TOF. Such a tissue
culture can, for example, be grown on plates or in liquid culture,
or be frozen for long term storage. The cells of tomato variety NUN
09225 TOF used to start the culture can be selected from any plant
part suitable for vegetative reproduction, or, in a particular
aspect, can be cells of an embryo, meristem, a cotyledon, a
hypocotyl, pollen, a leaf, an anther, a root, a root tip, a pistil,
a petiole, a flower, a fruit, a seed, or a stem. In another
particular aspect, the tissue culture does not contain somaclonal
variation or has reduced somaclonal variation. The skilled person
is familiar with methods to reduce or prevent somaclonal variation,
including regular reinitiation.
[0111] In one aspect, the disclosure provides a tomato plant
regenerated from the tissue or cell culture of tomato variety NUN
09225 TOF, wherein the regenerated plant is not significantly
different from tomato variety NUN 09225 TOF in all, or all but one,
two, or three, of the physiological and morphological
characteristics (e.g., determined at the 5% significance level when
grown under the same environmental conditions). Optionally, the
plant has one, two, or three the physiological and morphological
characteristics that are affected by a mutation or by
transformation. In another aspect, the disclosure provides a tomato
plant regenerated from the tissue or cell culture of tomato variety
NUN 09225 TOF, wherein the plant has all of the physiological and
morphological characteristics of said variety determined (e.g., 5%
significance level) when grown under the same environmental
conditions. Similarity or difference of a characteristic is
determined by measuring the characteristics on a representative
number of plants grown under the same environmental conditions,
determining whether type/degree characteristics are the same and
determining whether numerical characteristics are different at the
5% significance level.
[0112] Tomato variety NUN 09225 TOF, or its progeny, or a plant
having all physiological and/or morphological characteristics but
one, two, or three which are different from those of tomato variety
NUN 09225 TOF can also be reproduced using vegetative reproduction
methods. Therefore, the disclosure provides for a method of
producing a plant or plant part of tomato variety NUN 09225 TOF,
comprising vegetative propagation of tomato variety NUN 09225 TOF.
Vegetative propagation comprises regenerating a whole plant from a
plant part of variety NUN 09225 TOF, from a progeny or from or a
plant having all physiological and/or morphological characteristics
of said variety but one, two or three different characteristics,
such as a cutting, a cell culture, or a tissue culture.
[0113] The disclosure also provides methods of vegetatively
propagating a part of the plant of variety NUN 09225 TOF. In
certain aspects, the method comprises: (a) collecting tissue or
cells capable of being propagated from tomato variety NUN 09225
TOF; (b) cultivating said tissue or cells to obtain proliferated
shoots; and (c) rooting said proliferated shoots, to obtain rooted
plantlets. Steps (b) and (c) may also be reversed, i.e., first
cultivating said tissue to obtain roots and then cultivating the
tissue to obtain shoots, thereby obtaining rooted plantlets. The
rooted plantlets may then be further grown, to obtain plants. In
one aspect, the method further comprises step (d) growing plants
from said rooted plantlets. Therefore, the method also comprises
regenerating a whole plant from said part of the plant of variety
NUN 09225 TOF. In a particular aspect, the part of the plant to be
propagated is a cutting, a cell culture, or a tissue culture.
[0114] The disclosure also provides for a vegetatively propagated
plant of variety NUN 09225 TOF (or from progeny of tomato variety
NUN 09225 TOF or from or a plant having all but one, two or three
physiological and/or morphological characteristics of that
variety), wherein the plant has all of the morphological and
physiological characteristics of tomato variety NUN 09225 TOF, when
the characteristics are determined at the 5% significance level for
plants grown under the same conditions. In another aspect, the
propagated plant has all but one, two, or three of the
morphological and physiological characteristics of tomato variety
NUN 09225 TOF, when the characteristics are determined at the 5%
significance level for plants grown under the same conditions. A
part of said propagated plant or said propagated plant with one,
two, or three differences is also provided. In another aspect, the
propagated plant has all or all but 1, 2, or 3 of the physiological
and morphological characteristics of tomato variety NUN 09225 TOF
(e.g., as listed in Tables 1 and 2).
[0115] In another aspect, the disclosure provides a method for
producing a tomato plant part, preferably a fruit, comprising:
growing a plant of variety NUN 09225 TOF until it sets at least one
fruit, and collecting the fruit. Preferably, the fruit is collected
at harvest maturity. In another aspect, the fruit is collected when
the seed is ripe.
[0116] In another aspect, the plant of variety NUN 09225 TOF can be
produced by seeding directly in the soil (e.g., field) or by
germinating the seeds in controlled environment conditions (e.g.,
greenhouses, hydroponic cultures, etc.) and optionally then
transplanting the seedlings into the field. For example, the seed
can be sown into prepared seed beds where they will remain for the
entire production the crop (see, e.g., Hartz, et. al., University
of California Division of Agriculture and Natural Resources,
Publication 7228, 1-5). Tomatoes can be grown with a support system
such as poles (i.e., stakes) to keep the fruit from touching the
ground or as bushes without support. Alternatively, plastic row
covers can also be used to control the temperature. Mulches or
plastic tunnels can also be used to protect the plant from frost
(see, e.g., Strange, et. al., 2000, University of California
Division of Agriculture and Natural Resources, Publication 8017,
1-8). Tomato can also be grown entirely in greenhouses. Moreover,
said variety can be grown in hydroponic cultures as described
herein in, e.g., US 2008/0222949, which is herein incorporated by
reference in its entirety, and the skilled person is familiar with
various type of hydroponic cultures.
[0117] In another aspect, the plant and plant parts of tomato
variety NUN 09225 TOF and progeny of said variety are provided,
e.g., grown from seeds, produced by sexual or vegetative
reproduction, regenerated from the above-described plant parts, or
regenerated from cell or tissue culture of the tomato variety NUN
09225 TOF, in which the reproduced (seed propagated or vegetatively
propagated) plant has all of the physiological and morphological
characteristics of tomato variety NUN 09225 TOF, e.g., listed in
Tables 1 and 2. In one aspect, said progeny of tomato variety NUN
09225 TOF can be modified in one, two, or three characteristics, in
which the modification is a result of mutagenesis or transformation
with a transgene.
[0118] In other aspects, the disclosure provides a progeny plant of
variety NUN 09225 TOF such as a progeny plant obtained by further
breeding of tomato variety NUN 09225 TOF. Further breeding with
tomato variety NUN 09225 TOF includes selfing that variety one or
more times and/or cross-pollinating tomato variety NUN 09225 TOF
with another tomato plant or variety one or more times. In
particular, the disclosure provides for a progeny plant that
retains all the essential morphological and physiological
characteristics of tomato variety NUN 09225 TOF or, in another
aspect, a progeny plant that retains all, or all but one, two or
three, of the morphological and physiological characteristics of
tomato variety NUN 09225 TOF, optionally all or all but one, two or
three of the characteristics as listed in Tables 1 and 2, when
grown under the same environmental conditions, determined at the 5%
significance level for numerical characteristics. In a particular
aspect, the progeny is a first generation progeny, i.e., the ovule
or the pollen (or both) used in the crossing is an ovule or pollen
of variety NUN 09225 TOF, where the pollen comes from an anther and
the ovule comes from an ovary of variety NUN 09225 TOF. In another
aspect, the disclosure provides for a vegetative reproduction of
the variety and a plant having all, or all but 1, 2, or 3 of the
physiological and morphological characteristics of tomato variety
NUN 09225 TOF (e.g., as listed in Tables 1 and 2).
[0119] In still another aspect, the disclosure provides a method of
producing a tomato plant, comprising crossing a plant of tomato
variety NUN 09225 TOF with a second tomato plant at least once,
allowing seed to develop and optionally harvesting said progeny
seed. The skilled person can select progeny from said crossing.
Optionally, the progeny (grown from the progeny seed) is crossed
twice, thrice, or four, five, six, or seven times, and allowed to
set seed. In one aspect, the first "crossing" further comprises
planting seeds of a first and a second parent tomato plant, often
in proximity so that pollination will occur; for example, mediated
by insect vectors. Alternatively, pollen can be transferred
manually. Where the plant is self-pollinated, pollination may occur
without the need for direct human intervention other than plant
cultivation. After pollination the plant can produce seed.
[0120] The disclosure also provides a method for collecting pollen
of tomato variety NUN 09225 TOF, comprising collecting pollen from
a plant of variety NUN 09225 TOF. Alternatively, the method
comprises growing a plant of variety NUN 09225 TOF until at least
one flower contains pollen and collecting the pollen. In a
particular aspect, the pollen is collected when it is mature or
ripe. A suitable method for collecting pollen comprises collecting
anthers or the part of the anther that contains pollen, for
example, by cutting the anther or the part of the anther off.
Pollen can be collected in a container. Optionally, collected
pollen can be used to pollinate a tomato flower.
[0121] In still another aspect, the disclosure provides a method of
producing a plant, comprising selfing a plant of variety NUN 09225
TOF one or more times, and selecting a progeny tomato plant from
said selfing. In one aspect, the progeny plant retains all the
distinguishing characteristics of tomato variety NUN 09225 TOF
described above when grown under the same environmental conditions.
In a different aspect, the progeny plant comprises all of the
physiological and morphological characteristic of tomato variety
NUN 09225 TOF of Tables 1 and 2.
[0122] The disclosure also provides a method for developing a
tomato plant in a tomato breeding program, using a tomato plant
described herein, or its parts as a source of plant breeding
material. Suitable plant breeding techniques are recurrent
selection, backcrossing, pedigree breeding, mass selection,
mutation breeding, and/or genetic marker enhanced selection. In one
aspect, the method comprises crossing tomato variety NUN 09225 TOF
or progeny of said variety, or a plant comprising all but 1, 2, or
3 or more of the morphological and physiological characteristics of
tomato variety NUN 09225 TOF (e.g., as listed in Tables 1 and 2),
with a different tomato plant, and wherein one or more offspring of
the crossing are subject to one or more plant breeding techniques:
recurrent selection, backcrossing, pedigree breeding, mass
selection, mutation breeding and genetic marker enhanced selection
(see, e.g., Vidaysky and Czosnek, (1998) Phytopathology 88(9):
910-4). For breeding methods in general, see, e.g., Principles of
Plant Genetics and Breeding, 2007, George Acquaah, Blackwell
Publishing, ISBN-13: 978-1-4051-3646-4.
[0123] In one aspect, pedigree selection is used as a breeding
method for developing a tomato variety. Pedigree selection is also
known as the "Vilmorin System of Selection," see, e.g., Allard,
John Wiley & Sons, Inc., 1999, pp. 64-67. In general, selection
is first practiced among F2 plants. In the next season, the most
desirable F3 lines are first identified, then desirable F3 plants
within each line are selected. The following season and in all
subsequent generations of inbreeding, the most desirable families
are identified first, then desirable lines within the selected
families are chosen. A family refers to lines that were derived
from plants selected from the same progeny from the preceding
generation.
[0124] Thus, progeny in connection with pedigree selection are
either the generation (seeds) produced from the first cross (F1) or
selfing (S1), or any further generation produced by crossing and/or
selfing (F2, F3, etc.) and/or backcrossing (BC1, BC2, etc.) one or
more selected plants of the F1 and/or S1 and/or BC1 generation (or
plants of any further generation, e.g., F2) with another tomato
plant (and/or with a wild relative of tomato). Progeny may have all
the physiological and morphological characteristics of tomato
variety NUN 09225 TOF when grown under the same environmental
conditions and/or progeny may have (be selected for having) one or
more of the distinguishing characteristics of tomato variety NUN
09225 TOF.
[0125] In yet a further aspect, the disclosure provides for a
method of producing a new tomato plant. The method comprises
crossing tomato variety NUN 09225 TOF, or a plant comprising all
but 1, 2, or 3 of the morphological and physiological
characteristics of tomato variety NUN 09225 TOF (as listed in
Tables 1 and 2), or a progeny plant thereof, either as male or as
female parent, with a second tomato plant (or a wild relative of
tomato) one or more times, and/or selfing a tomato plant of variety
NUN 09225 TOF, or a progeny plant thereof, one or more times, and
selecting progeny from said crossing and/or selfing. The second
tomato plant may, for example, be a line or variety of the species
Solanum Lycopersicon, S. chilense, S. habrochaites, S. penelli, S.
peruvianum, S. pimpinellifolium or other Solanum species.
[0126] In a further aspect, tomato variety NUN 09225 TOF is used in
crosses with other, different, tomato varieties to produce first
generation (F1) tomato hybrid seeds and plants with superior
characteristics. In a particular aspect, the disclosure provides a
tomato seed and a plant produced by crossing a first parent tomato
plant with a second parent tomato plant, wherein at least one of
the first or second parent tomato plant is tomato variety NUN 09225
TOF. In another aspect, the tomato seed and plant produced are the
first filial generation (F1) tomato seed and plants produced by
crossing the plant of tomato variety NUN 09225 TOF with another
tomato plant.
[0127] The morphological and physiological characteristics (and the
distinguishing characteristics) of tomato variety NUN 09225 TOF are
provided in Tables 1 and 2, as collected in a trial according to
USDA and/or UPOV standards. Encompassed herein is also a plant
obtainable from tomato variety NUN 09225 TOF (e.g., by selfing
and/or crossing and/or backcrossing with said variety and/or
progeny of said variety) comprising all or all but one, two or
three of the physiological and morphological characteristics of
tomato variety NUN 09225 TOF listed in Tables 1 and 2 (as
determined at the 5% significance level for numerical
characteristics or identical for non-numerical characteristics)
when grown under the same environmental conditions and/or
comprising one or more (or all; or all except one, two or three)
characteristics when grown under the same environmental conditions.
The morphological and/or physiological characteristics may vary
somewhat with variation in the environment (such as temperature,
light intensity, day length, humidity, soil, fertilizer use), which
is why a comparison under the same environmental conditions is
preferred. Colors can best be measured using the Royal
Horticultural Society (RHS) Chart.
[0128] In another aspect, the disclosure provides a method of
producing a plant derived from a tomato variety NUN 09225 TOF,
comprising crossing a plant of variety NUN 09225 TOF either as a
male or female parent with a second plant or selfing tomato variety
NUN 09225 TOF or vegetative reproduction of tomato variety NUN
09225 TOF and collecting seeds from said crossing or selfing or
regenerating a whole plant from the vegetable cell-or tissue
culture. Also provided are seeds and/or plants obtained by this
method. All plants produced using tomato variety NUN 09225 TOF as a
parent are within the scope of the disclosure including plant parts
derived from tomato variety NUN 09225 TOF.
[0129] In further aspects, the method comprises growing a progeny
plant of a subsequent generation and crossing the progeny plant of
a subsequent generation with itself or a second plant and repeating
the steps for additional 3-10 generations to produce a plant
derived from tomato variety NUN 09225 TOF. The plant derived from
tomato variety NUN 09225 TOF may be an inbred line and the
aforementioned repeating crossing steps may be defined as
comprising sufficient inbreeding to produce the inbred line. By
selecting plants having one or more desirable traits of the line as
well as potentially other selected traits.
[0130] The disclosure provides for methods of producing plants
which retain all the morphological and physiological
characteristics of a plant described herein. The disclosure also
provides for methods of producing a plant comprising all but 1, 2,
or 3 or more of the morphological and physiological characteristics
of tomato variety NUN 09225 TOF (e.g., as listed in Tables 1 and
2), but which are still genetically closely related to said
variety. The relatedness can, for example, be determined by
fingerprinting techniques (e.g., making use of isozyme markers
and/or molecular markers such as Single-nucleotide polymorphism
(SNP) markers, amplified fragment length polymorphism (AFLP)
markers, microsatellites, minisatellites, Random Amplified
Polymorphic DNA (RAPD) markers, restriction fragment length
polymorphism (RFLP) markers and others). A plant is "closely
related" to tomato variety NUN 09225 TOF if its DNA fingerprint is
at least 80%, 90%, 95% or 98% identical to the fingerprint of that
variety. In a particular aspect, AFLP markers are used for DNA
fingerprinting (see, e.g., Vos et al. 1995, Nucleic Acid Research
23: 4407-4414). A closely related plant may have a Jaccard's
Similarity index of at least about 0.8, preferably at least about
0.9, 0.95, 0.98 or more (see, e.g., Sharifova, S., et. al., (2013),
Journal of Hort. Research, 21(1):83-89; Ince et al., (2010),
Biochem. Genet. 48:83-95; Parvathaneni et al., (2011), J. Crop Sci.
Biotech, 14 (1): 39-43; Pisanu, et. al., (2004), Acta Hort. 660,
83-89). The disclosure also provides a plant and a variety obtained
or selected by applying these methods on tomato variety NUN 09225
TOF. Such a plant may be produced by crossing and/or selfing, or
alternatively, a plant may simply be identified and selected
amongst plants of said variety, or progeny of said variety, e.g. by
identifying a variant within tomato variety NUN 09225 TOF or within
progeny of said variety (e.g., produced by selfing) which variant
differs from the variety described herein in one, two or three of
the morphological and/or physiological characteristics (e.g., in
one, two or three distinguishing characteristics), e.g. those
listed in Tables 1 and 2. In one aspect, the disclosure provides a
tomato plant having a Jaccard's Similarity index with tomato
variety NUN 09225 TOF of at least 0.8, e.g. at least 0.85, 0.9,
0.95, 0.98 or even at least 0.99.
[0131] In some aspects, the disclosure provides a tomato plant
comprising genomic DNA having at least 95%, 96%, 97%, 98% or 99%
sequence identity compared to the genomic DNA sequence of a plant
of variety NUN 09225 TOF as deposited under Accession Number NCIMB
______. In some aspects, the tomato plant further comprises all or
all but 1, 2, or 3 of the morphological and physiological
characteristics of tomato variety NUN 09225 TOF (e.g., as listed in
Tables 1 and 2). In other aspects, the tomato plant is a hybrid or
other derived from a seed or plant of variety NUN 09225 TOF. In
other aspects, the tomato plant comprises the distinguishing
characteristics of tomato variety NUN 09225 TOF.
[0132] For the purpose of this disclosure, the "sequence identity"
of nucleotide sequences, expressed as a percentage, refers to the
number of positions in the two optimally aligned sequences which
have identical residues (.times.100) divided by the number of
positions compared. A gap, i.e., a position in the pairwise
alignment where a residue is present in one sequence but not in the
other, is regarded as a position with non-identical residues. A
pairwise global sequence alignment of two nucleotide sequences is
found by aligning the two sequences over the entire length
according to the Needleman and Wunsch global alignment algorithm
described in Needleman and Wunsch, 1970, J. Mol. Biol.
48(3):443-53). A full implementation of the Needleman-Wunsch global
alignment algorithm is found in the needle program in The European
Molecular Biology Open Software Suite (see, e.g., EMBOSS, Rice et
al., Trends in Genetics June 2000, vol. 16, No. 6. pp.
276-277).
[0133] In one aspect, a plant of variety NUN 09225 TOF may also be
mutated (by e.g., irradiation, chemical mutagenesis, heat
treatment, etc.) and mutated seeds or plants may be selected in
order to change one or more characteristics of said variety.
Methods such as TILLING (Targeting Induced Local Lesions in
Genomes) may be applied to tomato populations in order to identify
mutants.
[0134] Similarly, tomato variety NUN 09225 TOF may be transformed
and regenerated, whereby one or more chimeric genes are introduced
into the variety or into a plant comprising all but 1, 2, 3, or
more of the morphological and physiological characteristics (e.g.,
as listed in Tables 1 and 2). Many useful traits can be introduced
into tomato variety NUN 09225 TOF by e.g., crossing a tomato
variety NUN 09225 TOF with a transgenic tomato plant comprising a
desired transgene, as well as by directly introducing a transgene
into tomato variety NUN 09225 TOF by genetic transformation
techniques.
[0135] Any pest or disease resistance genes may be introduced into
a plant of tomato variety NUN 09225 TOF, progeny of said variety or
into a plant comprising all but 1, 2, or 3 or more of the
morphological and physiological characteristics of tomato variety
NUN 09225 TOF (e.g., as listed in Tables 1 and 2). Resistance to
one or more of the following diseases or pests may be introduced
into the plant described herein: Colorado potato beetle, Southern
root knot nematode, Spider mites, Sugarfly beet army worm, Tobacco
flea beetle, Tomato hornworm, Tomato fruitworm, Whitefly, Bacterial
canker, Bacterial soft rot, Bacterial speck, Bacterial wilt
(Pseudomonas syringae pv. Tomato), Bacterial, Anthracnose
(Gloeosporium piperatum), Brown rot or corky root (Pyrenochaeta
lycopersici), Alternaria, Fusarium wilt (F. oxysporum races), Gray
leaf spot (Stemphylium spp), Late blight (Phytophthora infestans
races), and Leaf mold (Cladosporium fulvum races), Nematode
(Meloidogyne spp.), Verticillium Wilt (Verticillium dahliae),
Ralstonia solanacearum (Rs), Leveillula Taurica (Lt), and/or Oidium
neolycopersici (On). Other resistance genes, against pathogenic
viruses (e.g., Tomato Mosaic Virus (ToMV), Curly TOF Virus, Tomato
Mottle Virus, Potato Y Virus, Blotchey Ripening, Tobacco Etch
Virus, the various Tobacco Mosaic Virus races, Concentric cracking,
Tomato Spotted Wilt Virus (TSWV), Tomato Yellow Leaf Curl Virus
(TYLCV), Gold Fleck, Tomato Torrado Virus (ToTV)), fungi, bacteria,
nematodes, insects or other pests may also be introduced.
[0136] Genetic transformation may, therefore, be used to insert a
selected transgene into the tomato plants of the disclosure
described herein or may, alternatively, be used for the preparation
of transgenic tomato plants which can be used as a source of the
transgene(s), which can be introduced into tomato variety NUN 09225
TOF by e.g., backcrossing. A genetic trait which has been
engineered into the genome of a particular tomato plant may then be
moved into the genome of another tomato plant (e.g., another
variety) using traditional breeding techniques which are well-known
in the art. For example, backcrossing is commonly used to move a
transgene from a transformed tomato variety into an already
developed tomato variety and the resulting backcross conversion
plant will then comprise the transgene(s).
[0137] Any DNA sequences, whether from a different species or from
the same species, which are inserted into the genome using
transformation, are referred to herein collectively as
"transgenes." A "transgene" also encompasses antisense, or sense
and antisense sequences capable of gene silencing. Thus, the
disclosure also relates to transgenic plants of tomato variety NUN
09225 TOF. In some aspects, a transgenic plant of tomato variety
NUN 09225 TOF may contain at least one transgene but could also
contain at least 1, 2, 3, 4, or more transgenes.
[0138] Plant transformation involves the construction of an
expression vector which will function in plant cells. Such a vector
comprises DNA comprising a gene under control of, or operatively
linked to a regulatory element active in plant cells (e.g.,
promoter). The expression vector may contain one or more such
operably linked gene/regulatory element combinations. The vector
may be in the form of a plasmid and can be used alone or in
combination with other plasmids to provide transformed tomato
plants using transformation methods to incorporate transgenes into
the genetic material of the tomato plant(s). Transformation can be
carried out using standard methods, such as Agrobacterium
tumefaciens mediated transformation, electroporation, biolistics
particle delivery system, or microprojectile bombardment, followed
by selection of the transformed cells and regeneration into
plants.
[0139] Plants can also be genetically engineered, modified, or
manipulated to express various phenotypes of horticultural
interest. Through the transformation of tomato, the expression of
genes can be altered to enhance disease resistance, insect
resistance, herbicide resistance, stress tolerance, horticultural
quality, and other traits. Transformation can also be used to
insert DNA sequences which control or help control male sterility
or fertility restoration. DNA sequences native to tomato as well as
non-native DNA sequences can be transformed into tomato and used to
alter levels of native or non-native proteins. Various promoters,
targeting sequences, enhancing sequences, and other DNA sequences
can be inserted into the genome for the purpose of altering the
expression of proteins. Reduction of the specific activity of
specific genes (also known as gene silencing or gene suppression)
is desirable for several aspects of genetic engineering in
plants.
[0140] Genome editing is another method recently developed to
genetically engineer plants. Specific modification of chromosomal
loci or targeted mutation can be done through sequence-specific
nucleases (SSNs) by introducing a targeted DNA double strand break
in the locus to be altered. Examples of SSNs that have been applied
to plants are: finger nucleases (ZFNs), transcription
activator-like effector nucleases (TALENs), engineered homing
endonucleases or meganucleases, and clustered regularly interspaced
short palindromic repeats (CRISPR)/CRISPR-associated protein 9
(Cas9), see, e.g., Songstad, et. al., Critical Reviews in Plant
Sciences, 2017, 36:1, 1-23.
[0141] Thus, the disclosure also provides a method of producing a
tomato plant having a desired trait comprising mutating the plant
or plant part of variety NUN 09225 TOF and selecting a plant
comprising the desired trait, wherein the mutated plant retains all
or all but one of the physiological and morphological
characteristics of variety NUN 09225 TOF, optionally as described
in Tables 1 and 2, and contains the desired trait and wherein a
representative sample of seed of variety NUN 09225 TOF is deposited
under Accession Number NCIMB ______. In a further aspect, the
desired trait is yield, storage properties, color, flavor, size,
firmness, fruit quality, enhanced nutritional quality, post-harvest
quality, male sterility, herbicide tolerance, insect resistance,
pest resistance, disease resistance, environmental stress
tolerance, modified carbohydrate metabolism, modified protein
metabolism or ripening, or the mutation occurs in any of the
following genes acs2, acs4, rin, pp2c1, arf9, intense, myb12.
[0142] The disclosure also provides a method for inducing a
mutation in tomato variety NUN 09225 TOF comprising: [0143] a)
exposing the seed, plant, plant part, or cell of tomato variety NUN
09225 TOF to a mutagenic compound or to radiation, wherein a
representative sample of seed of said tomato variety is deposited
under Accession Number NCIMB; [0144] b) selecting the seed, plant,
plant part, or cell of tomato variety NUN 09225 TOF having a
mutation; and [0145] c) optionally growing and/or multiplying the
seed, plant, plant part, or cell of tomato variety NUN 09225 TOF
having the mutation.
[0146] The disclosure also provides a method of producing a tomato
plant having a desired trait, wherein the method comprises
transforming the tomato plant with a transgene that confers the
desired trait, wherein the transformed plant otherwise retains all
of the physiological and morphological characteristics of the plant
of variety NUN 09225 TOF and contains the desired trait. Thus, a
transgenic tomato plant is provided which is produced by the method
described above, wherein the plant otherwise has all of the
physiological and morphological characteristics of tomato variety
NUN 09225 TOF and the desired trait.
[0147] In another aspect, the disclosure provides a method of
producing a progeny of plant of variety NUN 09225 TOF further
comprising a desired trait, said method comprising transforming the
plant of tomato variety NUN 09225 TOF with at least one transgene
that confers the desired trait and/or crossing the plant of tomato
variety NUN 09225 TOF with a transgenic tomato plant comprising a
desired transgene so that the genetic material of the progeny that
resulted from the cross contains the desired transgene(s). Also
encompassed is the progeny produced by this method.
[0148] A desired trait (e.g., gene(s) conferring pest or disease
resistance, or tolerance for protection, etc.) can be introduced
into tomato variety NUN 09225 TOF, or progeny of said variety, by
transforming said variety or progeny of said variety with a
transgene that confers the desired trait, wherein the transformed
plant retains all or all but one, two or three of the morphological
and/or physiological characteristics of variety NUN 09225 TOF and
contains the desired trait. In another aspect, the transformation
or mutation confers a trait wherein the trait is yield, storage
properties, color, flavor, size, firmness, fruit quality, enhanced
nutritional quality, post-harvest quality, male sterility,
herbicide tolerance, insect resistance, pest resistance, disease
resistance, environmental stress tolerance, modified carbohydrate
metabolism, modified protein metabolism or occurs in the intense
gene. In a particular aspect, the specific transgene may be any
known in the art or listed herein, including, a polynucleotide
sequence conferring resistance to imidazolinone, sulfonylurea,
glyphosate, glufosinate, triazine, benzonitrile, cyclohexanedione,
phenoxy proprionic acid and L-phosphinothricin or a polynucleotide
conferring resistance to Colorado potato beetle, Southern root knot
nematode, Spider mites, Sugarfly beet army worm, Tobacco flea
beetle, Tomato hornworm, Tomato fruitworm, Whitefly, Bacterial
canker, Bacterial soft rot, Bacterial speck, Bacterial wilt
(Pseudomonas syringae pv. Tomato), Bacterial, Anthracnose
(Gloeosporium piperatum), Brown rot or corky root (Pyrenochaeta
lycopersici), Alternaria, Fusarium wilt (F. oxysporum races), Gray
leaf spot (Stemphylium spp.), Late blight (Phytophthora infestans
races), and Leaf mold (Cladosporium fulvum races), Nematode
(Meloidogyne spp.), Verticillium Wilt (Verticillium dahliae),
Ralstonia solanacearum (Rs), Leveillula Taurica (Lt), and/or Oidium
neolycopersici (On). Other resistance genes, against pathogenic
viruses (e.g., Tomato Mosaic Virus (ToMV), Curly TOF Virus, Tomato
Mottle Virus, Potato Y Virus, Blotchey Ripening, Tobacco Etch
Virus, the various Tobacco Mosaic Virus races, Concentric cracking,
Tomato Spotted Wilt Virus (TSWV), Tomato Yellow Leaf Curl Virus
(TYLCV), Gold Fleck, Tomato Torrado Virus (ToTV)), fungi, bacteria,
nematodes, insects or other pests may also be introduced.
[0149] By crossing and/or selfing, (one or more) single traits may
be introduced into tomato variety NUN 09225 TOF (e.g., using
backcrossing breeding schemes), while retaining the remaining
morphological and physiological characteristics of said variety
and/or while retaining one or more or all distinguishing
characteristics. A single trait converted plant may thereby be
produced. For example, disease resistance genes may be introduced,
genes responsible for one or more quality traits, yield, etc. Both
single genes (e.g., dominant or recessive) and one or more QTLs
(quantitative trait loci) may be transferred into tomato variety
NUN 09225 TOF by breeding with said variety.
[0150] Alternatively, a single trait converted plant or single
locus converted plant of variety NUN 09225 TOF may be produced by
(i) genetically transforming or mutating cells of tomato variety
NUN 09225 TOF; (ii) growing the cells into a plant; and (iii)
optionally selecting a plant that contains the desired single locus
conversion. The skilled person is familiar with various techniques
for genetically transforming a single locus in a plant cell, or
mutating said cells.
[0151] In another aspect, the disclosure provides a method of
introducing a single locus conversion, single trait conversion, or
a desired trait into tomato variety NUN 09225 TOF, comprising
introducing a single locus conversion, single trait conversion, or
a desired trait in at least one of the parents of tomato variety
NUN 09225 TOF; and crossing the converted parent with the other
parent of tomato variety NUN 09225 TOF to obtain seed of tomato
variety NUN 09225 TOF.
[0152] In another aspect, the step of introducing a single locus
conversion, single trait conversion, or a desired trait in at least
one of the parents comprises: [0153] a) crossing the parental line
of tomato variety NUN 09225 TOF with a second tomato plant
comprising the single locus conversion, the single trait
conversion, or the desired trait; [0154] b) selecting F1 progeny
plants that contain the single locus conversion, the single trait
conversion, or the desired trait; [0155] c) crossing said selected
progeny plants of step b) with the parental line of step a) to
produce a backcross progeny plant; [0156] d) selecting backcross
progeny plants comprising the single locus conversion, the single
trait conversion or the desired trait and otherwise all or all but
one, two or three of the morphological and physiological
characteristics the parental line of step a) to produce selected
backcross progeny plants; and [0157] e) optionally repeating steps
c) and d) one or more times in succession to produce selected
second, third or fourth or higher backcross progeny plants
comprising the single locus conversion, the single trait conversion
or the desired trait and otherwise all or all but one, two or three
of the morphological and physiological characteristics the parental
line of step a) to produce selected backcross progeny plants, when
grown in the same environmental conditions. The disclosure further
relates to plants obtained by this method.
[0158] In another aspect, the step of introducing a single locus
conversion, single trait conversion, or a desired trait in at least
one of the parents comprises: [0159] a) obtaining a cell or tissue
culture of cells of the parental line of tomato variety NUN 09225
TOF; [0160] b) genetically transforming or mutating said cells;
[0161] c) growing the cells into a plant; and [0162] d) optionally
selecting plants that contain the single locus conversion, the
single trait conversion, or the desired trait.
[0163] In another aspect, the disclosure provides a method of
introducing a single locus conversion, single trait conversion, or
a desired trait into tomato variety NUN 09225 TOF comprising:
[0164] a) obtaining a combination of a parental lines of tomato
variety NUN 09225 TOF, optionally through reverse synthesis of
breeding lines, [0165] b) introducing a single locus conversion,
single trait conversion, or a desired trait in at least one of the
parents of step a); and [0166] c) crossing the converted parent
with the other parent of step a) to obtain seed of tomato variety
NUN 09225 TOF.
[0167] In another method, the step of introducing a single locus
conversion, single trait conversion, or a desired trait in at least
one of the parents comprises genetically transforming or mutating
cells the parental line of tomato variety NUN 09225 TOF; growing
the cells into a plant; and optionally selecting plants that
contain the single locus conversion, the single trait conversion,
or the desired trait.
[0168] In any of the above methods, where the single locus
conversion, single trait conversion, or a desired trait concerns a
trait, the trait may be yield or pest resistance or disease
resistance. In one aspect, the trait is disease resistance and the
resistance are conferred to Colorado potato beetle, Southern root
knot nematode, Spider mites, Sugarfly beet army worm, Tobacco flea
beetle, Tomato hornworm, Tomato fruitworm, Whitefly, Bacterial
canker, Bacterial soft rot, Bacterial speck, Bacterial wilt
(Pseudomonas syringae pv. Tomato), Bacterial, Anthracnose
(Gloeosporium piperatum), Brown rot or corky root (Pyrenochaeta
lycopersici), Alternaria, Fusarium wilt (F. oxysporum races), Gray
leaf spot (Stemphylium spp.), Late blight (Phytophthora infestans
races), and Leaf mold (Cladosporium fulvum races), Nematode
(Meloidogyne spp.), Verticillium Wilt (Verticillium dahliae),
Ralstonia solanacearum (Rs), Leveillula Taurica (Lt), and/or Oidium
neolycopersici (On). Other resistance genes, against pathogenic
viruses (e.g., Tomato Mosaic Virus (ToMV), Curly TOF Virus, Tomato
Mottle Virus, Potato Y Virus, Blotchey Ripening, Tobacco Etch
Virus, the various Tobacco Mosaic Virus races, Concentric cracking,
Tomato Spotted Wilt Virus (TSWV), Tomato Yellow Leaf Curl Virus
(TYLCV), Gold Fleck, Tomato Torrado Virus (ToTV)), fungi, bacteria,
nematodes, insects or other pests may also be introduced.
[0169] The disclosure also provides a plant having one, two or
three physiological and/or morphological characteristics which are
different from those of tomato variety NUN 09225 TOF and which
otherwise has all the physiological and morphological
characteristics of said variety, wherein a representative sample of
seed of tomato variety NUN 09225 TOF is deposited under Accession
Number NCIMB ______. In particular, variants which differ from
tomato variety NUN 09225 TOF, in none, one, two or three of the
characteristics mentioned in Tables 1 and 2 are encompassed.
[0170] The disclosure also provides a tomato plant comprising at
least a first set of the chromosomes of tomato variety NUN 09225
TOF, a sample of seed of said variety is deposited under Accession
Number NCIMB ______; optionally further comprising a single locus
conversion or a mutation, wherein said plant has essentially all of
the morphological and physiological characteristics of the plant
comprising at least a first set of the chromosomes of said variety.
In another aspect, this single locus conversion confers a trait,
wherein the trait is yield, storage properties, color, flavor, male
sterility, herbicide tolerance, insect resistance, pest resistance,
disease resistance, environmental stress tolerance, modified
carbohydrate metabolism, modified protein metabolism or ripening,
or the mutation occurs in any of the following genes acs2, acs4,
rin, pp2c1, arf9, intense, myb12.
[0171] In one aspect, the disclosure provides for a haploid plant
and/or a doubled haploid plant of tomato variety NUN 09225 TOF, or
of a plant having all but one, two or three physiological and/or
morphological characteristics of tomato variety NUN 09225 TOF, or
progeny of any of these, is encompassed herein. Haploid and doubled
haploid (DH) plants can, for example, be produced by cell or tissue
culture and chromosome doubling agents and regeneration into a
whole plant. DH production chromosome doubling may be induced using
known methods, such as colchicine treatment or the like. In one
aspect, the method comprises inducing a cell or tissue culture with
a chromosome doubling agent and regenerating the cells or tissues
into a whole plant.
[0172] In another aspect, the disclosure comprises a method for
making doubled haploid cells of tomato variety NUN 09225 TOF,
comprising making doubled haploid cells from haploid cells from the
plant of plant part of tomato variety NUN 09225 TOF with a
chromosome doubling agent, such as colchicine treatment (see, e.g.,
Nikolova V, Niemirowicz-Szczytt K (1996) Acta Soc Bot Pol
65:311-317).
[0173] In another aspect, the disclosure provides for haploid
plants and/or doubled haploid plants derived from tomato variety
NUN 09225 TOF that, when combined, make a set of parents of tomato
variety NUN 09225 TOF. The haploid plant and/or the doubled haploid
plant of variety NUN 09225 TOF can be used in a method for
generating parental lines of tomato variety NUN 09225 TOF.
[0174] The disclosure also provides methods for determining the
identity of parental lines of plants described herein, in
particular the identity of the female line. US 2015/0126380, which
is hereby incorporated by reference, relates to a non-destructive
method for analyzing maternal DNA of a seed. In this method, the
DNA is dislodged from the seed coat surface and can be used to
collect information on the genome of the maternal parent of the
seed. This method for analyzing maternal DNA of a seed comprises
contacting a seed with a fluid to dislodge DNA from the seed coat
surface, and analyzing the DNA thus dislodged from the seed coat
surface using methods known in the art. The skilled person is thus
able to determine whether a seed has grown on a plant of variety
NUN 09225 TOF or is a progeny of said variety, because the seed
coat of the seed is a maternal tissue genetically identical to
tomato variety NUN 09225 TOF. In one aspect, the present disclosure
relates to a seed coat comprising maternal tissue of tomato variety
NUN 09225 TOF. In another aspect, the disclosure relates to a
tomato seed comprising a maternal tissue of tomato variety NUN
09225 TOF. In another particular aspect, the disclosure provides
for a method of identifying the female parental line of tomato
variety NUN 09225 TOF by analyzing the seed coat of a seed of that
variety. In another aspect, the disclosure provides for a method of
determining whether a seed is grown on tomato variety NUN 09225 TOF
by analyzing the seed coat or another maternal tissue of said
seed.
[0175] Using methods known in the art such as "reverse synthesis of
breeding lines" or "reverse breeding," it is possible to produce
parental lines for a hybrid plant such as tomato variety NUN 09225
TOF. A skilled person can take any individual heterozygous plant
(called a "phenotypically superior plant" in Example 2 of US
2015/0245570, which is hereby incorporated by reference in its
entirety; tomato variety NUN 09225 TOF is such plant) and generate
a combination of parental lines (reverse breeding parental lines)
that, when crossed, produce the variety NUN 09225 TOF. It is not
necessary that the reverse breeding parental lines are identical to
the original parental lines. Such new breeding methods are based on
the segregation of individual alleles in the spores produced by a
desired plant and/or in the progeny derived from the
self-pollination of that desired plant, and on the subsequent
identification of suitable progeny plants in one generation, or in
a limited number of inbred cycles. Such a method is known from US
2015/0245570 or from Wijnker et al., Nature Protocols Volume: 9,
Pages: 761-772 (2014) DOI: doi:10.1038/nprot.2014.049. Thus, the
disclosure provides a method for producing parental lines for a
hybrid organism (e.g., tomato variety NUN 09225 TOF), comprising in
one aspect: a) defining a set of genetic markers present in a
heterozygous form (H) in a partially heterozygous starting
organism; b) producing doubled haploid lines from spores of the
starting organism; c) genetically characterizing the doubled
haploid lines thus obtained for the said set of genetic markers to
determine whether they are present in a first homozygous form (A)
or in a second homozygous form (B); and d) selecting at least one
pair of doubled haploid lines that have complementary alleles for
at least a subset of the genetic markers, wherein each member of
the pair is suitable as a parental line for the hybrid
organism.
[0176] In another aspect, the method for producing parental lines
of a hybrid organisms, e.g., of tomato variety NUN 09225 TOF, which
when crossed reconstitute the genome of tomato variety NUN 09225
TOF, comprising: [0177] a) defining a set of genetic markers that
are present in a heterozygous form (H) in a partially heterozygous
starting organism; [0178] b) producing at least one further
generation from the starting organism by self-pollination (e.g., F2
or F3 generation); [0179] c) selecting at least one pair of progeny
organisms in which at least one genetic marker from the set is
present in a complementary homozygous form (B vs. A, or A vs. B);
and [0180] d) optionally repeating steps b) and c) until at least
one pair of progeny organisms that have complementary alleles for
at least a subset of the genetic markers has been selected as
parental lines for a hybrid.
[0181] Thus, in one aspect, the disclosure relates to a method of
producing a combination of parental lines of a plant of variety NUN
09225 TOF, comprising making doubled haploid cells from haploid
cells or seed of that plant; and optionally crossing these parental
lines to produce and collecting seeds. In another aspect, the
disclosure relates to a combination of parental lines produced by
this method. In still another aspect, the combination of parental
lines can be used to produce a seed or plant of variety NUN 09225
TOF when these parental lines are crossed. In still another aspect,
the disclosure relates to a combination of parental lines from
which a seed or plant having all physiological and/or morphological
characteristics of tomato variety NUN 09225 TOF (when the
characteristics are determined at the 5% significance level for
plants grown under the same conditions).
[0182] The disclosure also provides a method for producing parental
lines for hybrid NUN 09225 TOF comprising: genetically
characterizing a doubled haploid line from tomato variety NUN 09225
TOF to determine whether one or more genetic markers are present in
a first homozygous form or in a second homozygous form in said
line, wherein the one or more genetic markers are present in a
heterozygous form in tomato variety NUN 09225 TOF; and selecting at
least one pair of doubled haploid lines that have complementary
alleles for the one or more the genetic markers, wherein each
member of the pair is suitable as a parental line for a hybrid
organism, optionally this method further comprises defining a set
of genetic markers present in a heterozygous form in tomato variety
NUN 09225 TOF; and producing doubled haploid lines from tomato
variety NUN 09225 TOF. Doubled haploid lines generated as described
herein can be used in such a method.
[0183] The disclosure also provides a combination of parental lines
which, when crossed, produce a seed or plant having all
physiological and/or morphological characteristics of tomato
variety NUN 09225 TOF but one, two or three which are different
(when grown under the same environmental conditions), as well as a
seed or plant having all physiological and/or morphological
characteristics of tomato variety NUN 09225 TOF but one, two or
three which are different (when the characteristics are determined
at the 5% significance level for plants grown under the same
conditions).
[0184] In another aspect, a combination of a male and a female
parental line of tomato variety NUN 09225 TOF can be generated by
methods described herein, for example, through reverse synthesis of
breeding lines.
[0185] In another aspect, the disclosure provides a method of
determining the genotype of a plant described herein comprising
detecting in the genome (e.g., a sample of nucleic acids) of the
plant at least a first polymorphism or an allele. The skilled
person is familiar with many suitable methods of genotyping,
detecting a polymorphism or detecting an allele including SNP
(Single Nucleotide Polymorphism) genotyping, restriction fragment
length polymorphism identification (RFLP) of genomic DNA, random
amplified polymorphic detection (RAPD) of genomic DNA, amplified
fragment length polymorphism detection (AFLP), polymerase chain
reaction (PCR), DNA sequencing, allele specific oligonucleotide
(ASO) probes, and hybridization to DNA microarrays or beads.
Alternatively, the entire genome could be sequenced. The method
may, in certain aspects, comprise detecting a plurality of
polymorphisms in the genome of the plant, for example by obtaining
a sample of nucleic acid from a plant and detecting in said nucleic
acids a plurality of polymorphisms. The method may further comprise
storing the results of the step of detecting the plurality of
polymorphisms on a computer readable medium.
[0186] Also provided is a plant part obtainable from variety NUN
09225 TOF or from progeny of said variety or from a plant having
all but one, two or three tomato variety 09225 TOF or from a
vegetatively propagated plant of variety NUN 09225 TOF (or from its
progeny or from a plant having all or all but one, two or three
physiological and/or morphological characteristics which are
different from those of tomato variety NUN 09225 TOF), wherein the
plant part is a fruit, a harvested fruit, a part of a fruit, a
leaf, a part of a leaf, pollen, an ovule, a cell, a petiole, a
shoot or a part thereof, a stem or a part thereof, a root or a part
thereof, a root tip, a cutting, a seed, a part of a seed, seed-coat
or another maternal tissue which is part of a seed grown on tomato
variety NUN 09225 TOF, or a hypocotyl, a cotyledon, a scion, a
stock, a rootstock, a pistil, an anther, or a flower or a part
thereof.
[0187] A part of the plant of variety NUN 09225 TOF (or of progeny
of said variety or of a plant having all physiological and/or
morphological characteristics but one, two or three which are
different from those of said variety) encompasses any cells,
tissues, organs obtainable from the seedlings or plants, such as
but not limited to: a tomato fruit or a part thereof, a cutting, a
hypocotyl, a cotyledon, seed coat, or pollen.
[0188] Such a plant part of variety NUN 09225 TOF can be stored
and/or processed further. The disclosure thus also provides for a
food or feed product comprising one or more of such parts from
variety NUN 09225 TOF or from progeny of said variety, or from a
derived variety, such as a plant having all but one, two or three
physiological and/or morphological characteristics of tomato
variety NUN 09225 TOF. Preferably, the plant part is a tomato fruit
or part thereof and/or an extract from a fruit or another plant
part described herein comprising at least one cell of tomato
variety NUN 09225 TOF. The food or feed product may be fresh or
processed, e.g., dried, grinded, powdered, pickled, chopped,
cooked, roasted, in a sauce, in a sandwich, pasted, pureed or
concentrated, juiced, pickled, canned, steamed, boiled, fried,
blanched and/or frozen, etc.
[0189] In another aspect, the disclosure provides for a tomato
fruit of variety NUN 09225 TOF, or a part of a fruit of said
variety. The fruit can be in any stage of maturity, for example,
immature or mature. In another aspect, the disclosure provides for
a container comprising or consisting of a plurality of harvested
tomato fruits or parts of fruits of said variety, or fruits of
progeny thereof, or fruits of a derived variety.
[0190] Marketable tomato fruits are generally sorted by size and
quality after harvest. Alternatively, the tomato fruits can be
sorted by expected shelf life, pH, or Brix.
[0191] In another aspect, the plant, plant part or seed of tomato
variety NUN 09225 TOF is inside a container, for example,
containers such as cans, boxes, crates, bags, cartons, Modified
Atmosphere Packaging, films (e.g., biodegradable films), etc.
comprising a plant or a part of a plant (fresh and/or processed) or
a seed of tomato variety NUN 09225 TOF. In a particular aspect, the
container comprises a plurality of seeds of tomato variety NUN
09225 TOF or a plurality of plant parts of tomato variety NUN 09225
TOF.
[0192] Tomato variety NUN 09225 TOF may also be grown for use as
rootstocks (stocks) or scions. Typically, different types of
tomatoes are grafted to enhance disease resistance, which is
usually conferred by the rootstock, while retaining the
horticultural qualities usually conferred by the scion. It is not
uncommon for grafting to occur between cultivated tomato varieties
and related tomato species. Methods of grafting and vegetative
propagation are well-known in the art.
[0193] In another aspect, the disclosure provides to a plant
comprising a rootstock or scion of tomato variety NUN 09225
TOF.
[0194] All documents (e.g., patent publications) are herein
incorporated by reference in their entirety, including the
following cited references: [0195] Naktuinbow Calibration book for
Tomato (Lycopersicon esculentum Mill), 2010 [0196] UPOV, Guidelines
for the Conduct of Tests for Distinctness, Uniformity and
Stability, TG/13/11, world-wide web at upov.int under
edocs/tgdocs/en/tg044.pdf. [0197] US Department of Agriculture,
Agricultural Marketing Service, Objective Description of Variety
Tomato (Solanum lycopersicum or Lycopersicon esculentum Mill),
world wide web at
ams.usda.gov/services/plant-variety-protection/pvpo-c-forms, under
tomato. [0198] Acquaah, Principles of Plant Genetics and Breeding,
2007, Blackwell Publishing, ISBN-13: 978-1-4051-3646-4. [0199]
Bhatia, P., et al., Tissue Culture Studies of Tomato (Lycopersicum
esculentum), Plant Cell, Tissue and Organ Culture, 2004, vol. 78,
pp. 1-21. [0200] Hartz, et. al., "Processing Tomato Production in
California," University of California Division of Agriculture and
Natural Resources, 2008, Publication 7228, pp. 1-5. [0201] Ince, A.
G., et al., Genetic Relationship Within and Between Capsicum
Species, Biochem Genet, 2010, vol. 48, pp. 83-95. [0202] Needleman,
S. B., et. al., A General Method Applicable to the Search for
Similarities in the Amino Acid Sequence of Two Proteins, Journal of
Molecular Biology, 1970, vol. 48(3), pp. 443-53. [0203] Nikolova,
V., et. al., Diploidization of Cucumber (Cucumis sativus L.)
Haploids by Colchini Treatment, Acta Societas Botanicorum Poloniae,
1996, vol. 65, pp. 311-317. [0204] Pisanu, A. B., et. al., "Yield
and Biometric Characteristics of 9 Clones Selected from the
Population of "Spinoso sardo" Artichokes, Acta Hort., 2004, ISHS
660, pp. 83-89. [0205] Rice et al., EMBOSS: The European Molecular
Biology Open Software Suite, Trends in Genetics, 2000, vol. 16,
Issue 6. pp. 276-277. [0206] Sharifova, S., et. al., "Assessment of
Genetic Diversity in Cultivated Tomato (Solanum lycopersicum L.)
Genotypes Using RAPD Primers", Journal of Horticultural Research,
2013, vol. 21, no. 1, pp. 83-89. [0207] Strange, et. al.,
"Fresh-Market Tomato Production in California," University of
California Division of Agriculture and Natural Resources, 2000,
Publication 8017, pp. 1-8. [0208] Vidaysky F, Czosnek H., 1998,
Tomato Breeding Lines resistant and tolerant to tomato yellow leaf
curl virus issued from Lycopersicum hirsutum, Phytopathology,
September; 88(9):910-4. [0209] Vos, P., et al., AFLP: A New
Technique for DNA Fingerprinting 1995, Nucleic Acids Research,
1995, vol. 23, No. 21, pp. 4407-4414. [0210] Wijnker, E., et al.,
Hybrid Recreation by Reverse breeding in Arabidopsis thaliana,
Nature Protocols, 2014, vol. 9, pp. 761-772. [0211] US2008/0222949
[0212] U.S. Pat. No. 9125353 [0213] US 2002/0010953 [0214] U.S.
Pat. No. 6,060,648 [0215] EP 1057401 [0216] EP 1428425 [0217] US
2008/0222949 [0218] US 2015/0126380 [0219] US 2015/0245570
Development of Tomato Variety NUN 09225 TOF
[0220] The hybrid variety NUN 09225 TOF was developed from a male
and female proprietary inbred line of Nunhems, selected mainly for
its big round size and intense trait (full flesh). The female and
male parents were crossed to produce hybrid (F1) seeds of tomato
variety NUN 09225 TOF. The seeds of tomato variety NUN 09225 TOF
can be grown to produce hybrid plants and parts thereof (e.g.,
tomato fruit). The hybrid variety NUN 09225 TOF can be propagated
by seeds or vegetatively.
[0221] The hybrid variety is uniform and genetically stable. This
has been established through evaluation of horticultural
characteristics. Several hybrid seed production events resulted in
no observable deviation in genetic stability. Coupled with the
confirmation of genetic stability of the female and male parents
the Applicant has concluded that tomato variety NUN 09225 TOF is
uniform and stable.
Deposit Information
[0222] A total of 2500 seeds of the hybrid variety NUN 09225 TOF
will be deposited according to the Budapest Treaty by Nunhems B. V.
on ______, at the NCIMB Ltd., Ferguson Building, Craibstone Estate,
Bucksburn, Aberdeen AB21 9YA, United Kingdom (NCIMB). The deposit
will be assigned NCIMB number ______. A statement indicating the
viability of the sample will be provided. A deposit of tomato
variety NUN 09225 TOF and of the male and female parent line is
also maintained at Nunhems B. V. Tomato variety NUN 09225 TOF has a
seed lot number of 26954302002.
[0223] The deposit will be maintained in NCIMB for a period of 30
years, or 5 years after the most recent request, or for the
enforceable life of the patent whichever is longer and will be
replaced if it ever becomes nonviable during that period. Access to
the deposit will be available during the pendency of this
application to persons determined by the Director of the U.S.
Patent Office to be entitled thereto upon request. All restrictions
imposed by the depositor on the availability to the public of the
deposited material will be irrevocably removed upon the granting of
the patent. Applicant does not waive any rights granted under this
patent on this application or under the Plant Variety Protection
Act (7 U.S.C. .sctn. 2321 et seq.). Accordingly, the requirements
of 37 CFR .sctn. 1.801-1.809 will be satisfied.
Characteristics of Tomato Variety NUN 09225 TOF
[0224] The most similar variety to NUN 09225 TOF is NUN 09085 TOF,
a variety from Nunhems B. V. In Table 1, a comparison between
tomato variety NUN 09225 TOF and the Reference Variety is shown
based on a trial in the Netherlands during the trial season 2017.
For numerical characteristics averages were calculated. For
non-numerical characteristics, the type/degree were determined. In
Table 2, the disease resistances of tomato variety NUN 09225 TOF
and the Reference Variety are shown. In Table 3, the distinguishing
characteristics between tomato variety NUN 09225 TOF and the
Reference Variety are shown.
[0225] In one aspect, the disclosure provides a plant having the
physiological and morphological characteristics of tomato variety
NUN 09225 TOF as presented in Tables 1 and 2.
TABLE-US-00001 TABLE 1 Characteristics of Tomato Variety NUN 09225
TOF and the Reference Variety Application Variety Reference Variety
Characteristics (NUN 09225 TOF) (NUN 09085 TOF) Seedling:
Anthocyanin coloration of hypocotyl: Present Present 1 = Absent; 2
= Present Plant: Growth type: 1 = Determinate; 2 = Indeterminate
Indeterminate Indeterminate; 3 = Semi-determinant; 4 = Semi
indeterminant Plant height: 1 = Very short; 3 = Short; Long Long 5
= Medium; 7 = Long; 9 = Very long Stem: Anthocyanin coloration: 1 =
Absent or Absent or very weak Absent or very weak very weak; 3 =
Weak; 5 = Medium; 7 = Strong; 9 = Very strong Length of internode:
3 = Short; 5 = Medium Medium Medium; 7 = Long Leaf: Attitude: 1 =
Erect; 2 = Semi-erect; 5 = Horizontal Semi-drooping Horizontal; 7 =
Semi-drooping; 9 = Drooping Length: 3 = Short; 5 = Medium; 7 =
Medium Medium Long Width: 3 = Narrow; 5 = Medium; 7 = Medium Medium
Broad Type of blade: 1 = Pinnate; 2 = Bipinnate Bipinnate Bipinnate
Size of leaflets (in middle of leaf): 1 = Small Medium Very small;
3 = Small; 5 = Medium; 7 = Large; 9 = Very large Intensity of green
color: 1 = Very light; Dark Medium 3 = Light; 5 = Medium; 7 = Dark;
9 = Very dark Glossiness: 3 = Weak; 5 = Medium; 7 = Medium Medium
to strong Strong Blistering: 3 = Weak; 5 = Medium; 7 = Medium
Medium Strong Attitude of petiole of leaflet in relation Horizontal
Semi-erect to main axis: 3 = Semi-erect; 5 = Horizontal; 7 =
Semi-drooping Flower: Inflorescence type: 1 = Mainly Mainly
uniparous Mainly uniparous uniparous; 2 = Equally uniparous and
multiparous; 3 = Mainly multiparous Color: 1 = Yellow; 2 = Orange
Yellow Yellow Pubescence of style: 1 = Absent or Present Present
very scarce; 9 = Present Peduncle: Abscission layer: 1 = Absent; 2
= Present Present Present Peduncle length: 3 = Short; 5 = Short
Medium Medium; 7 = Long Fruit: Green shoulder (before maturity): 1
= Absent Absent Absent; 9 = Present Intensity of green color
excluding No green shoulder Medium shoulder (before maturity): 1 =
Very light; 3 = Light; 5 = Medium; 7 = Dark; 9 = Very dark Green
stripes (before maturity): 1 = Absent Absent Absent; 9 = Present
Size: 1 = Very small; 2 = Very small to Medium to large Medium to
large small; 3 = Small; 4 = Small to medium; 5 = Medium; 6 = Medium
to large; 7 = Large; 8 = Large to very large; 9 = Very large Weight
of ripened fruit (grams): 130 g 100-120 g Shape in longitudinal
section: 1 = Oblate Obovate Flattened; 2 = Oblate; 3 = Circular; 4
= Oblong; 5 = Cylindrical; 6 = Elliptic; 7 = Cordate; 8 = Ovate; 9
= Obovate; 10 = Pyriform; 11 = Obcordate Ribbing at peduncle end: 1
= Absent or Weak Absent or very weak very weak; 3 = Weak; 5 =
Medium; 7 = Strong; 9 = Very strong Depression at peduncle end: 1 =
Medium Absent or very weak Absent or very weak; 3 = Weak; 5 =
Medium; 7 = Strong; 9 = Very strong Size of peduncle scar: 1 = Very
small; Medium Medium 3 = Small; 5 = Medium; 7 = Large; 9 = Very
large Size of blossom scar: = Very small; 3 = Small Small Small; 5
= Medium; 7 = Large; 9 = Very large Shape at blossom end: 1 =
Indented; 2 = Flat Flat to pointed Indented; 2 = Flat; 3 = Flat to
pointed; 4 = Conical Size of core in cross section; 1 = Very Very
large Medium small; 3 = Small; 5 = Medium; 7 = Large; 9 = Very
thick Thickness of pericarp: 1 = Very thin; Thick Medium 3 = Thin;
5--Medium; 7 = Thick; 9 = Very thick Number of locules: 1 = Only
two; 2 = Two or three Two or three Two or three; 3 = Three or four;
4 = Four, five, or six; 5 = More than six Color at maturity: 1 =
Cream; 2 = Red Red Yellow; 3 = Orange; 4 = Pink; 5 = Red; 6 =
Brown; 7 = Green Color of flesh (at maturity): 1 = Cream; Red Red 2
= Yellow; 3 = Orange; 4 = Pink; 5 = Red; 6 = Brown; 7 = Green
Glossiness of skin: 1 = Weak; 2 = Medium Weak Medium; 3 = Strong
Color of epidermis: 1 = Colorless; 2 = colorless Yellow Yellow
Firmness: 1 = Very soft; 3 = Soft; 5 = Firm Firm Medium; 7 = Firm;
9 = Very firm Shelf life: 1 = Very short; 3 = Short; 5 = Medium, 14
days Long, 25 days Medium; 7 = Long/9 = Very long Maturity: Time of
flowering (50% of the plants Medium Medium with at least one open
flower from seed sowing): 3 = Early; 5 = Medium; 7 = Late Time of
maturity: 1 = Very early; 3 = Medium Late Early; 5 = Medium; 7 =
Late; 9 = Very late
TABLE-US-00002 TABLE 2 Disease Resistances of Tomato Variety NUN
09225 TOF and the Reference Variety Application Variety Reference
Variety (NUN 09225 (NUN Resistances TOF) 09085 TOF) Meloidoygne
incognita (Mi) Susceptible Susceptible 1 = Susceptible; 2 =
Intermediate Resistant; 3 = Resistant Verticillium dahliae Race 0
Present Absent 1 = Absent; 9 = Present Fusarium oxysporum f. sp.
Present Present Lycopersici Race 0 1 = Absent; 9 = Present Fusarium
oxysporum f. sp. Present Present Lycopersici Race 1 1 = Absent; 9 =
Present Fusarium oxysporum f. sp. Present Absent Lycopersici Race 2
1 = Absent; 9 = Present Fusarium oxysporum f. sp. radicis Present
Present lycopersici 1 = Absent; 9 = Present Fulvia fulva Race 0
Fulvia fulva Group A Absent Present 1 = Absent; 9 = Present Fulvia
fulva Group B Absent Present 1 = Absent; 9 = Present Fulvia fulva
Group C Absent Present 1 = Absent; 9 = Present Fulvia fulva Group D
Absent Present 1 = Absent; 9 = Present Fulvia fulva Group E Present
Present 1 = Absent; 9 = Present Tomato Mosaic Virus (ToMV) Strain 0
Present Present 1 = Absent; 9 = Present Tomato Mosaic Virus (ToMV)
Strain 1 Present Present 1 = Absent; 9 = Present Tomato Mosaic
Virus (ToMV) Strain 2 Present Present 1 = Absent; 9 = Present
Tomato Mosaic Virus (ToMV) Strain Absent Absent 1-2 1 = Absent; 9 =
Present Phytophthora infestans Absent Absent 1 = Absent; 9 =
Present Pyrenochaeta lycopersici Not tested Not tested 0 = not
tested; 1 = Absent; 9 = Present Stemphylium spp. Not tested Not
tested 0 = not tested; 1 = Absent; 9 = Present Pseudomonas syringae
pv tomato Not tested Not tested 0 = not tested; 1 = Absent; 9 =
Present Ralstonia solanacearum Race 1 Not tested Not tested 0 = not
tested; 1 = Absent; 9 = Present Tomato Yellow Leaf Curl Virus
Absent Absent (TYLCV) 1 = Absent; 9 = Present Tomato Spotted Wilt
Virus Race 0 Absent Absent 1 = Absent; 9 = Present Leveillula
Taurica Not tested Not tested 0 = not tested; 1 = Absent; 9 =
Present Oidium neolypersici Present Absent 0 = not tested; 1 =
Absent; 9 = Present Torrado Virus Absent Absent 0 = not tested; 1 =
Absent; 9 = Present
TABLE-US-00003 TABLE 3 Distinguishing Characteristics between
Tomato Variety NUN 09225 TOF and the Reference Variety Application
Variety Reference Variety Characteristics (NUN 09225 TOF) (NUN
09085 TOF) Leaf: Attitude: 1 = Erect; 2 = Semi-erect; 5 =
Horizontal Semi-drooping Horizontal; 7 = Semi-drooping; 9 =
Drooping Size of leaflets (in middle of leaf): 1 = Small Medium
Very small; 3 = Small; 5 = Medium; 7 = Large; 9 = Very large
Intensity of green color: 1 = Very light; Dark Medium 3 = Light; 5
= Medium; 7 = Dark; 9 = Very dark Peduncle: Peduncle length: 3 =
Short; 5 = Short Medium Medium; 7 = Long Fruit: Intensity of green
color excluding No green shoulder Medium shoulder (before
maturity): 1 = Very light; 3 = Light; 5 = Medium; 7 = Dark; 9 =
Very dark Shape in longitudinal section: 1 = Oblate Obovate
Flattened; 2 = Oblate; 3 = Circular; 4 = Oblong; 5 = Cylindrical; 6
= Elliptic; 7 = Cordate; 8 = Ovate; 9 = Obovate; 10 = Pyriform; 11
= Obcordate Ribbing at peduncle end: 1 = Absent or Weak Absent or
very weak very weak; 3 = Weak; 5 = Medium; 7 = Strong; 9 = Very
strong Depression at peduncle end: 1 = Medium Absent or very weak
Absent or very weak; 3 = Weak; 5 = Medium; 7 = Strong; 9 = Very
strong Shape at blossom end: 1 = Indented; 2 = Flat Flat to pointed
Indented; 2 = Flat; 3 = Flat to pointed; 4 = Conical Size of core
in cross section; 1 = Very Very large Medium small; 3 = Small; 5 =
Medium; 7 = Large; 9 = Very thick Thickness of pericarp: 1 = Very
thin; Thick Medium 3 = Thin; 5--Medium; 7 = Thick; 9 = Very thick
Glossiness of skin: 1 = Weak; 2 = Medium Weak Medium; 3 = Strong
Color of epidermis: 1 = Colorless; 2 = colorless Yellow Yellow
Shelf life: 1 = Very short; 3 = Short; 5 = Medium, 14 days Long, 25
days Medium; 7 = Long/9 = Very long
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