Faecalibacterium Prausnitzii Strains For Treating And Preventing Gastrointestinal Pain

Abstract

The present invention relates to a bacterial strain of the Faecalibacterium prausnitzii species selected from a bacterial strain belonging to one of the phylogroups I, II and III, for use in the treatment and/or prevention of visceral abdominal pain in an individual. The present invention also concerns compositions comprising said bacterial strains as well as specific strains as such.

Description

FIELD OF THE INVENTION

[0001] The present invention concerns Faecalibacterium prausnitzii strains for use in the treatment and prevention of visceral abdominal pain in an individual, in particular of gastrointestinal pain of non-inflammatory origin.

[0002] The present invention also concerns a composition, in particular an oral composition, comprising specific Faecalibacterium prausnitzii strains as well as new Faecalibacterium prausnitzii strains per se.

PRIOR ART

[0003] Visceral abdominal pain, and in particular gastrointestinal pain, is a very common disorder that affects millions of people every year all over the world and is a leading cause of patient visits to clinics. Abdominal pain is present on questioning of 75 percent of otherwise healthy adolescent students (Hyams et al. J. Pediatr. 1996; 129:220) and in about half of all adults (Heading; Scand J Gastroenterol Suppl. 1999; 231:3).

[0004] Such visceral pain can originate from various origins such as peptic disease, pancreatitis in alcoholics, inflammatory bowel disease, appendicitis, ruptured viscus, acute volvulus or even gastritis, biliary colic, gastroenteritis, splenic rupture, hepatic laceration or small bowel rupture.

[0005] However, gastrointestinal disorders, in particular non-inflammatory gastrointestinal disorders, and more particularly Irritable Bowel Syndrome (IBS), are the most frequent reasons for visceral abdominal pain in an individual.

[0006] IBS is one of the 25 most wide-spread recognized pathologies of the gastrointestinal tract. In the USA and in the first five European countries (France, Germany, the United Kingdom, Spain and Italy), in 2009, IBS affected more than 24 million persons. IBS accounts for 40 to 60% of referrals to gastroenterology outpatient clinics (Jones et al., Gut 2000; 47(Suppl 2): ii1-ii19).

[0007] Three subgroups of IBS patients have been defined based on the predominant bowel habit: constipation-predominant (c-IBS), diarrhea-predominant (d-IBS) or alternating between 25 the two (a-IBS). The definition and diagnostic criteria for IBS have been formalized in the "Rome Criteria" (Drossman et al. 1999, Gut 45:Suppl II: 1-81).

[0008] The pathophysiology of IBS is believed to involve alterations in the brain-gut axis. However, the mechanisms by which these changes lead to IBS remain poorly understood.

[0009] IBS is in particular characterized by a colonic hypersensitivity. This pain sensitivity is usually studied using variation of balloon distention in the rectum, a procedure in which a balloon is inserted into the rectum and slowly inflated. This common complaint is a crucial feature because of its significant impact on a patient's quality of life and lack of efficient therapies.

[0010] However, the current treatments against IBS and visceral abdominal pain are disappointing.

[0011] There is thus a need for new substances, in particular probiotics, or compositions for the treatment and/or prevention of visceral abdominal pain, in particular gastrointestinal pain, in an individual.

[0012] There is also a need for new substances, in particular probiotics, or compositions for preventing and/or treating visceral abdominal pain in an individual suffering from a non-inflammatory gastrointestinal disorder, in particular from an Irritable Bowel Syndrome (IBS).

[0013] More particularly, there is a need in the prior art for new substances, in particular probiotics, or compositions that are able to diminish the sensitivity of nociceptors in the gastrointestinal tract of an individual, in particular in individuals suffering from a colonic hypersensitivity.

SUMMARY OF THE INVENTION

[0014] The present invention aims to provide novel substances, in particular probiotics, and compositions for treating and/or preventing visceral abdominal pain in an individual, more particularly gastrointestinal pain of non-inflammatory origin.

[0015] According to the inventors' experimental results, specific Faecalibacterium prausnitzii (F. prausnitzii) strains, belonging to three particular phylogroups previously described (Lopez-Siles et al., 2012), possess the unexpected ability to diminish visceral abdominal pain in an individual. As illustrated in the Examples and discussed further, the inventors have demonstrated that F. prausnitzii strains outside of these phylogroups, such as the strain CNCM I-4541, do not possess such advantageous properties.

[0016] The properties of the F. prausnitzii strains of the invention have been demonstrated in the examples by the inventor's in two different models: a Neonatal Maternal Separation (NMS) colonic hypersensitivity (CHS) mouse model and a Partial Restraint Stress (PRS) rat model.

[0017] Strains according to the invention are more particularly able to diminish the sensitivity of nociceptors in the gastrointestinal tract of an individual and consequently to prevent and/or reduce colonic hypersensitivity of said individual.

[0018] According to a first object, the present invention relates to a bacterial strain of the Faecalibacterium prausnitzii species selected from a bacterial strain belonging to one of the phylogroups I, II and III according to the invention, for use in the treatment and/or prevention of visceral abdominal pain in an individual.

[0019] These strains identified by the inventors are thus probiotics that can be used for the above-indicated purposes.

[0020] An individual according to the invention is a mammal, in particular a human.

[0021] Visceral abdominal pain is pain associated with organs of the abdomen of the individual.

[0022] The visceral abdominal pain is in particular a gastrointestinal pain, in particular a colonic pain.

[0023] A bacterial strain of the Faecalibacterium prausnitzii species according to the invention is different from the strain A2-165.

[0024] According to a preferred embodiment, bacterial strains according to the invention for use as indicated here above are selected from the group consisting of bacterial strains deposited to the CNCM under the accession numbers I-4542, I-4544, I-4540, I-4574, I-4543, I-4575, I-4573, I-4644 and I-4546, and in particular the bacterial strain deposited to the CNCM under the accession number I-4573.

[0025] According to an embodiment, the visceral pain according to the invention is caused by a gastrointestinal disorder, in particular a non-inflammatory gastrointestinal disorder.

[0026] A gastrointestinal disorder of the invention, in particular according to this embodiment, can be a gastrointestinal hypersensitivity, in particular a colonic hypersensitivity, and is preferably an Irritable Bowel Syndrome, in particular an alternating-type Irritable Bowel Syndrome.

[0027] In particular, such gastrointestinal disorder according to the invention can be selected from the group consisting of IBS and constipation.

[0028] According to another embodiment, a bacterial strain for use according to the invention is comprised in a composition comprising a physiologically acceptable medium, preferably in an oral composition, and most preferably in a food supplement.

[0029] Thus, according to another object, the present invention relates to a composition comprising, in a physiologically acceptable medium, at least one bacterial strain of Faecalibacterium prausnitzii selected from the group consisting of bacterial strains deposited to the CNCM under the accession numbers I-4542, I-4544, I-4540, I-4574, I-4543, I-4575, I-4573, I-4644 and I-4546.

[0030] In a preferred embodiment, said bacterial strain of the Faecalibacterium prausnitzii species is the bacterial strain deposited to the CNCM under the accession numbers I-4573.

[0031] The term "physiologically acceptable medium" is understood to mean a medium that is compatible with the organism of the individual to whom said composition is intended to be administered. It can for example be any non-toxic solvent such as water. In particular, said medium is compatible with an oral administration.

[0032] A composition of the invention is preferably for the oral route and in particular in the form of a food supplement.

[0033] A composition of the invention for the oral route can be selected from the group consisting of a food product, a drink, a pharmaceutical, a nutraceutical, a food additive, a food supplement and a dairy product. It can in particular be a food supplement.

[0034] The inventors have also indentified new Faecalibacterium prausnitzii strains having the specific abilities discussed previously.

[0035] Consequently, a further object of the invention consists in an isolated bacterial strain selected from the group consisting of bacterial strains deposited to the CNCM under the accession numbers I-4542, I-4544, I-4540, I-4574, I-4543, I-4575, I-4573, I-4644 and I-4546.

[0036] According to a preferred embodiment, said bacterial strain is the bacterial strain deposited to the CNCM under the accession number I-4573.

[0037] As demonstrated in the examples, a Faecalibacterium prausnitzii strain of the invention has no negative impact in an individual not suffering from visceral abdominal pain according to the invention and is thus perfectly safe for use.

FIGURES' LEGENDS

[0038] FIG. 1A illustrates the variation of the Vicero Motrice Response (VMR--mV/s) of a colon depending on the Distension volume (4) of a balloon inserted into the rectum and slowly inflated, in non-handled (NH) (normal lines) or Maternal separated (MS) (bold lines) mice pre-treated with PBS (dotted lines) or with CNCM I-4573 (full lines) bacteria according to the invention.

[0039] FIG. 1B illustrates the variation of the Vicero Motrice Response (VMR--mV/s) of a colon depending on the Distension volume (tt) of a balloon inserted into the rectum and slowly inflated, in non-handled (NH) (normal lines) or Maternal separated (MS) (bold lines) mice pre-treated with PBS (dotted lines) or with CNCM I-4541 (full lines) bacteria outside of the invention.

[0040] FIG. 2 illustrates 35 Faecalibacterium prausnitzii strains and their distribution in or outside of the three phylogroups of the invention.

[0041] For illustrative purposes, Faecalibacterium prausnitzii strains CNCM I-4541 (SEQ ID No: 16) and 513E3 (SEQ ID No: 17) are outside the three phylogroups of the invention. The method for obtaining said distribution is discussed further below.

[0042] FIG. 3A illustrates the effect of F. prausnitzii CNCM I-4573 strain on the number of abdominal cramps/5 minutes in Partial Restraint Stress (PRS) (PRS-4573--continuous slashed line; n=12 rats) or basal rats models (Basal-4573--continuous lines with circles; n=12 rats) compared to PRS or basal rats treated with PBS (respectively PRS-PBS--dotted slashed lines and Basal-PBS--dotted lines with circles; n=18 rats per group), depending on the Distension volume (mL) generated with an arterial embolectomy catheter introduced into the rectum of the rats.

[0043] FIG. 3B illustrates the effect of F. prausnitzii CNCM I-4541 strain outside of the invention on the number of abdominal cramps/5 minutes in Partial Restraint Stress (PRS) (PRS-4573--continuous slashed line; n=12 rats) or basal rats models (Basal-4573--continuous lines with circles; n=12 rats) compared to PRS or basal rats treated with PBS (respectively PRS-PBS--dotted slashed lines and Basal-PBS--dotted lines with circles; n=18 rats per group), depending on the Distension volume (mL) generated with an arterial embolectomy catheter introduced into the rectum of the rats.

DETAILED DESCRIPTION OF THE INVENTION

[0044] The present inventors have performed a huge amount of work with the view of identifying the ability of specific Faecalibacterium prausnitzii strains to treat and/or prevent visceral abdominal pain, and in particular gastrointestinal pain, in an individual.

[0045] The inventors have indeed unexpectedly determined that Faecalibacterium prausnitzii strains belonging to three phylogroups defined further exhibit the ability to diminish the sensitivity of nociceptors in the gastrointestinal tract of an individual.

[0046] The bacterial strains according to the invention can prevent and/or reduce visceral abdominal pain, in particular gastrointestinal pain, in an individual, in particular in an individual suffering from a gastrointestinal non-inflammatory disorder, and in particular from a colonic hypersensitivity, such as in IBS.

[0047] It is demonstrated herein that bacterial strains of the invention are in particular able to reduce the Vicero Motrice Response (VMR) of a colon when a balloon inserted into the rectum is slowly inflated in an IBS mouse model pre-treated with a bacterial strain of the invention. Said VMR is brought back to a normal intensity, since the VMR level observed after administration of a strain according to the invention is similar to the VMR observed in mice not suffering from colonic hypersensitivity.

[0048] Faecalibacterium prausnitzii Strains of the Invention

[0049] F. prausnitzii is a major member of the Firmicutes phylum and is part of the most abundant commensal bacteria in the healthy human large intestine microbiota. F. prausnitzii is an extremely oxygen sensitive (EOS) bacterium and is thus difficult to cultivate, even in anaerobic conditions (Duncan et al. 2002, Int. J. Syst. Evol. Microbiol. 52(Pt 6): 2141-6 and Lopez-Siles et al. Appl. Environ Microbiol. 2012 January;78(2):420-8). F. prausnitzii is in particular known as being one of the most abundant butyrate-producing bacterium in the human gastrointestinal tract, the short chain fatty acid butyrate being very important in gut physiology, systemic functions and beneficial effects for human health (Macfarlane and Macfarlane (2011), J. Clin. Gastroenterol. 45 Suppl: S120-7).

[0050] F. prausnitzii is also known for having anti-inflammatory and protective effects in murine models of acute and chronic colitis, i.e. in inflammatory disorders (Martin et al., Inflamm Bowel Dis. 2014 March; 20(3):417-30 and Sokol et al., Proc Natl Acad Sci USA. 2008 Oct. 28;105(43):16731-6).

[0051] Recently, diminished prevalence and abundance of F. prausnitzii have been reported in non-inflammatory intestinal disorders. A negative correlation has indeed been observed between the abundance of F. prausnitzii bacteria and IBS symptoms, in particular in alternating-type IBS.

[0052] Emerging evidences suggest that perturbation of the gastrointestinal microbiota, and in particular dysbiosis, plays a role in the pathophysiology of IBS (Ringel and Maharshak, Am. J. Physiol. Gastrointest. Liver Physiol. 2013 Oct. 15; 305(8): G529-41).

[0053] The ability of the F. prausnitzii strains of the invention to prevent visceral abdominal pain, and in particular gastrointestinal pain, in an individual is specific to the strains identified by the inventors, and constituting the three phylogroups of the invention.

[0054] Such specific antinociceptive activity is illustrated in the examples, wherein a comparative test has been performed with a F. prausnitzii strain not part of the three phylogroups of the invention, i.e. the strain CNCM I-4541. As demonstrated in these examples, this strain does not possess the antinociceptive activity of the strains of the invention.

[0055] Method for Obtaining Phylogroups of the Invention

[0056] Isolated Faecalibacterium prausnitzii strains have been classified by the inventors in three phylogroups illustrated in FIG. 2 according to 16S rRNA sequences.

[0057] Full-length 16S rRNA gene sequences of each strain were obtained or determined and compared one to the other.

[0058] Full-length 16S rRNA sequences of 17 F. prausnitzii strains are indicated as sequences SEQ ID No: 1 to 17.

[0059] DNA was extracted from isolated colonies of the different F. prausnitzii strains by alkaline lysis in 50 .mu.L of NaOH 0.5 M during 30 min and 50 .mu.l of Tris 1M pH7 and 100 .mu.L H.sub.2O were added.

[0060] 16S rRNA sequences were amplified and PCR products purified with the Wizard SV Gel. PCR Clean-Up system (Promega) was used to obtain bidirectional partial 16S rRNA gene sequences by using primers. All DNA sequences were confirmed by sequencing (Eurofins MWG Operon, Ebersberg, Germany).

[0061] Multiple sequence alignment was performed with hierarchical clustering for 16S rRNA gene sequence full-length construction (http://multalin.toulouse.inrafr/multalin) (COrpet F., Nucleic Acids Res. 1988; 16:10881-10890).

[0062] Phylogenetic analysis of the 16S rRNA gene was then performed using MEGA6 software package (http://www.megasoftware.net/). The evolutionary history was inferred using the Neighbor-Joining method (Saitou N. and Nei M., Mol Biol Evol. 1987; 4:406-425).

[0063] The optimal tree with the sum of branch length=0.24930207 is shown in FIG. 2. The percentage of replicate trees in which the associated taxa clustered together in the bootstrap test (500 replicates) are shown next to the branches (Zharkikh A. and Li WH, Mol Phylogenet Evol. 1995; 4:44-63).

[0064] The tree is drawn to scale, with branch lengths in the same units as those of the evolutionary distances used to infer the phylogenetic tree. The evolutionary distances were computed using the Maximum Composite Likelihood method (Tamura et al., Proc Natl Acad Sci USA. 2004; 101:11030-11035) and are in the units of the number of base substitutions per site.

[0065] The analysis involved 35 nucleotide sequences. Codon positions included were lst+2nd+3rd+Non-coding. All positions containing gaps and missing data were eliminated.

[0066] There were a total of 1091 positions in the final dataset.

[0067] Eubacterium desmolans was used to root the tree (SEQ ID No: 18). In sequence SEQ ID No: 18, `n` is used to represent any ambiguous nucleotides.

[0068] In order to verify if a given F. prausnitzii strain can be classified in one of the phylogroups I to III according to the invention, its 16S rRNA sequence needs to be compared through the MEGA6 software package discussed here-above directly to the one of the present invention.

[0069] According to an embodiment, a bacterial strain of the invention is selected from phylogroup I.

[0070] Bacterial strains belonging to phylogroup I can be represented by the strains consisting of: [0071] bacterial strains deposited to the CNCM under the accession numbers I-4542, I-4544 and I-4540; and [0072] bacterial strains of reference HTF-75H, S13A7, S3G1, L2-15, L2-39, L2-61 and L2-6.

[0073] Preferably, a bacterial strain belonging to phylogroup I is selected from the group consisting of bacterial strains deposited to the CNCM under the accession numbers I-4542, I-4544 and I-4540.

[0074] According to an embodiment, a bacterial strain of the invention is selected from phylogroup II.

[0075] Bacterial strains belonging to phylogroup I1 can for example be selected from the group consisting of bacterial strains of reference HTF-60C, HTF-I, HTF-E, HTF-C, HTF-A, HTF-B and HTF-F.

[0076] According to an embodiment, a bacterial strain of the invention is selected from phylogroup III.

[0077] Bacterial strains belonging to phylogroup III can be represented by the strains consisting of: [0078] bacterial strains deposited to the CNCM under the accession numbers I-4574, I-4543, I-4575, I-4573, I-4644 and I-4546; and [0079] bacterial strains of reference S3C12, S9G3, S9D8, S3L/3, S4L/4, M21/2 and S10H3, with the proviso that the bacterial strain is different from the bacterial strain A2-165.

[0080] Preferably, a bacterial strain belonging to phylogroup III is selected from the group consisting of bacterial strains deposited to the CNCM under the accession numbers I-4574, I-4543, I-4575, I-4573, I-4644 and I-4546.

[0081] Bacterial strains of reference ATCC 27768, A2-165, L2-15, L2-39, L2-6, L2-61, M21/2, S3L/3, S4L/4, HTF-A, HTF-B, HTF-C, HTF-E, HTF-F, HTF-I, HTF-60C and HTF-75H can be obtained as indicated in Lopez-Siles et al., Appl. Environ. Microbiol. 2012, 78(2):420.

[0082] In a preferred embodiment, a bacterial strain of the invention is selected from phylogroups I and/or III. More particularly, a bacterial strain of the invention is selected from phylogroups I and/or III with the proviso that the bacterial strain is different from the bacterial strain A2-165.

[0083] According to an embodiment, a bacterial strain of the invention is selected from the group consisting of bacterial strains deposited to the CNCM under the accession numbers I-4542, I-4544, I-4540, I-4574, I-4543, I-4575, I-4573, I-4644 and I-4546.

[0084] A bacterial strain of the invention is preferably the bacterial strain deposited to the CNCM under the accession number I-4573.

[0085] The inventors have also indentified new Faecalibacterium prausnitzii strains having the specific abilities discussed previously.

[0086] Consequently, the present invention further concerns an isolated bacterial strain selected from the group consisting of bacterial strains deposited to the CNCM under the accession numbers I-4542, I-4544, I-4540, I-4574, I-4543, I-4575, I-4573, I-4644 and I-4546.

[0087] Said bacterial strain is in particular the bacterial strain deposited to the CNCM under the accession number I-4573.

[0088] A suitable daily dose of a bacterial strain according to the invention is from 10.sup.3 to 10.sup.12 colony forming units (cfu), more preferably from 10.sup.7 to 10.sup.11 cfu as a medicament, for example as a daily dose equivalent to 10.sup.10 cfu.

[0089] A Faecalibacterium prausnitzii strain of the invention is for use in the treatment and/or prevention of visceral abdominal pain in an individual.

[0090] The strains of the invention are probiotics whose activity lies in the gut. A probiotic bacterium according to the invention denotes a bacterium which ingested live in adequate quantities can exert beneficial effects on the human health.

[0091] These strains consequently need to be administered alive to the gut.

[0092] The bacteria strains of the invention can be administered to the gut of an individual to be treated by different ways, i.e. by the oral, rectal or parenteral route. A bacterium according to the invention is preferably administered by the oral or rectal route, more preferably by the oral route.

[0093] According to a preferred embodiment, a bacterial strain of the invention is comprised in a composition comprising a physiologically acceptable medium. Such composition is preferably for the oral route, and in particular in the form of a food supplement.

[0094] Compositions

[0095] The present invention further concerns a composition comprising, in a physiologically acceptable medium, at least one bacterial strain of Faecalibacterium prausnitzii of the invention.

[0096] More particularly, according to an embodiment, the present invention relates to a composition comprising, in a physiologically acceptable medium, at least one bacterial strain of Faecalibacterium prausnitzii selected from the group consisting of bacterial strains deposited to the CNCM under the accession numbers I-4542, I-4544, I-4540, I-4574, I-4543, I-4575, I-4573, I-4644 and I-4546.

[0097] Preferably, a composition according to the invention comprises at least one bacterial strain deposited to the CNCM under the accession number I-4573.

[0098] A composition according to the invention is intended for the gastrointestinal tract, in particular the gut.

[0099] Consequently, a composition according to the invention is selected from an oral, rectal or parenteral composition. A composition of the invention is preferably an oral or rectal composition, more preferably an oral composition.

[0100] According to an embodiment, a composition of the invention is an oral composition, i.e. is intended for oral administration to a subject.

[0101] Such composition can be in the form of a suspension, tablet, pill, capsule, granulate or powder.

[0102] The composition according to the invention for the oral route can be selected from the group consisting of a food product, a drink, a pharmaceutical, a nutraceutical, a food additive, a food supplement or a dairy product, and is in particular a food supplement.

[0103] According to a preferred embodiment, a composition according to the invention is a food supplement.

[0104] Food supplement for oral administration may be present in capsules, gelatin capsules, soft capsules, tablets, sugar-coated tablets, pills, pastes, pastilles, gums, drinkable solutions or emulsions, a syrup or a gel.

[0105] Advantageously, a composition according to the invention, intended for oral administration, can be provided with a coating resistant to gastric juice, so as to ensure that the bacterial strain of the invention comprised in said composition can pass through the stomach undamaged. The release of the bacterial strain can thus takes place for the first time in the upper intestinal tract.

[0106] A food supplement according to the invention can also include a sweetener, a stabilizer, an antioxidant, an additive, a flavouring agent and/or a colorant.

[0107] The formulation thereof is carried out by means of the usual methods for producing sugar-coated tablets, gel capsules, gels, hydrogels for controlled release, emulsions, tablets or capsules.

[0108] A composition according to the invention can also be in the form of a nutritional composition.

[0109] A nutritional composition according to the invention is in the form of a yogurt, a cereal bar, a breakfast cereal, a dessert, a frozen food, a soup, a pet food, a liquid suspension, a powder, a tablet, a gum or a candy.

[0110] In a further embodiment of the invention, a composition containing a bacterial strain of the invention is administered intrarectally.

[0111] A rectal administration preferably takes place in the form of a suppository, enema or foam.

[0112] A composition according to the invention contains an amount of bacterial strains of the invention equivalent to between 10.sup.3 and 10.sup.12 cfu/g (dry weight basis), more preferably between 10.sup.6 and 10.sup.9 cfu/g.

[0113] A composition according to the invention can further comprise at least one of: antioxidants, fish oils, DHA, EPA, vitamins, minerals, phytonutrients, protein, fat, probiotics, and combinations thereof.

[0114] The present invention further concerns the use of at least one bacterial strain of the Faecalibacterium prausnitzii species selected from a bacterial strain belonging to at least one of the phylogroups I, II and III, for the treatment and/or prevention of visceral abdominal pain in an individual.

[0115] The present invention also concerns the use of a composition, preferably an oral composition, comprising, in a physiologically acceptable medium, at least one bacterial strain of the Faecalibacterium prausnitzii species selected from a bacterial strain belonging to at least one of the phylogroups I, II and III, for the treatment and/or prevention of visceral abdominal pain in an individual.

[0116] The invention will be described below in greater details using the following examples which are given for illustrative purposes only.

[0117] All references to percentages are percentages by weight unless otherwise stated.

EXAMPLES

[0118] F. prausnitzii strains according to the invention have been tested for their ability to have a direct impact on visceral pain, and more particularly on colonic hypersensitivity.

[0119] Bacterial Strains Isolation and Growth Conditions

[0120] The F. prausnitzii strains isolates used in the present example all are of human fecal origin from healthy patients. Said isolates were obtained from the highest countable dilution of human fecal samples in roll tubes of anaerobic M2GSC medium as discussed in Lopez-Sites et al. (Appl. Environ Microbiol. 2012; 78:420-428).

[0121] Tested F. prausnitzii strains isolates were grown in LYBHI medium (Brain-Heart infusion medium supplemented with 0.5% yeast extract) (Dyfco, Detroit, USA) supplemented with 1 mg/mL cellobiose (Sigma-Aldrich Chemie Gmbh, Buchs, Switzerland), 1 mg/mL maltose (Sigma-Aldrich) and 0.5 mg/mL cysteine (Sigma-Aldrich) at 37.degree. C. in anaerobic chamber.

[0122] A. In a first series of tests, a Neonatal Maternal Separation (NMS) colonic hypersensitivity (CHS) mouse model is used.

[0123] a. NMS CHS Mouse Model

[0124] Pregnant C57Bl/6J mice have been purchased from Janvier laboratories (Le Genest Saint Isle, France).

[0125] After birth, wild-type C57Bl/6J were isolated from their mother from D2 to D14, three hours a day, from 9 a.m. to 12 a.m. At nine-week old age, male mice were orally treated each day for nine days with 200 .mu.L of 10.times.10.sup.10 CFU/mL of: [0126] the strain CNCM I-4573 according to the invention; [0127] the strain CNCM I-4541 outside the invention; or [0128] PBS as control.

[0129] All the experiments were performed on the last day of treatment at D10.

[0130] The same bacteria treatment was administered to non-NMS CHS mice as a control.

[0131] NMS treatment induced an increased Vicero Motrice Response (VMR) in the absence of any significant alteration in gut wall macroscopic integrity or colonic mucosa inflammation (Coutinho et al. (2002), Am. J. Physiol. Gastrointest. Liver Physiol. 282(2): G307-16).

[0132] As described in Christianson et al. 2007 (Nat Protoc 2(10): 2624-31), colonic sensitivity was assessed by quantifying visceromotor response with abdominal electromyography (EMG) recordings in response to colorectal distension.

[0133] The inventors also validated this model as being non-inflammatory by checking the cytokines pattern by measurement of 13 types of cytokines in serum samples of stressed (MS: Maternal separated) and non-stressed (NH: Non-handled) mice.

[0134] It was observed that there are no differences in cytokine pattern induced by the chronic stress.

[0135] Moreover, NMS does not induce changes in colonic macroscopic damage, loss of architectural epithelium, goblet cell depletion, oedema/ulceration or inflammatory cell infiltrates.

[0136] Furthermore, NMS does not induce changes in body weight, colon weight and length and in spleen weight when stressed (MS) mice are compared to the control group (PBS).

[0137] b. Statistical Analysis

[0138] Statistical analysis was completed using GraphPad software (GraphPad Software, La Jolla, Calif., USA). All data were expressed as mean+/-SEM.

[0139] MS mice displaying VMR values lower than mean minus two SEM for all distension volumes were considered as non-sensitized and excluded from the analysis.

[0140] For VMR analysis in model validation, a two-way (Volume & Treatment) ANOVA followed by Bonferroni post-hoc test for multiple comparisons was used.

[0141] P<0.05 was considered statistically significant.

[0142] c. Results

[0143] The results obtained are presented in FIGS. 1A and 1B.

[0144] As it can be seen in FIG. 1A, control Maternal separated (MS) mice, to whom only PBS was administered, present a high VMR, two times higher than the VMR of the non-handled (NH) mice, treated with CNCM I-4573 bacteria or only with PBS.

[0145] More importantly, it can be seen that MS mice to whom CNCM I-4573 bacteria of the invention were administered possess a VMR statistically similar to the one of NH mice, all along the growth of the Volume Distension.

[0146] On the contrary, in FIG. 1B, there is no statistical difference between the results obtained with MS mice only treated with PBS or treated with CNCM I-4541 bacteria.

[0147] Treatment based on the administration of F. prausnitzii strain CNCM I-4573 according to the invention led to a specific and significant decrease of the VMR in the tested mice as well as a prevention of the increase of the VMR.

[0148] On the contrary, the treatments based on the control tests (NH mice) and on the administration of F. prausnitzii strain CNCM I-4541 present no effect on the VMR of all the tested mice.

[0149] The present results clearly demonstrate that F. prausnitzii strains according to the invention have a specific and unexpected beneficial effect on visceral abdominal pain.

[0150] B. In a second series of tests, a Partial Restreint Stress (PRS) rat model is used.

[0151] a. Partial Restraint Stress (PRS) Rat Model

[0152] Animal preparation was performed as previously described in Ait-Belgnaoui et al.; Gut 55, 1090-1094 (2006).

[0153] Briefly, under general anesthesia induced by i.p. administration of 0.6 mg/kg acepromazine (Calmivet, Vetoquinol, Lure, France) and 120 mg/kg ketamine (Imalgene 1000, Merial, Lyon, France), female Wistar rats (Rosztoczy, A. et al.; Neurogastroenterol. Motil. Off. J. Eur. Gastrointest. Motil. Soc. 15, 679-686 (2003)) were equipped with three groups of three NiCr wire electrodes (60 cm in length, 80 nm in diameter) implanted into the abdominal external oblique muscle, 2 cm above the inguinal ligament.

[0154] Electrodes were exteriorized at the neck level by a glass tube attached to the skin.

[0155] During the ten days previous to the stress, 1 ml of .+-.1.times.10.sup.9 CFU or PBS were daily intragastrically administrated.

[0156] The study groups are as follows: [0157] the strain CNCM I-4573 according to the invention; [0158] the strain CNCM I-4541 outside the invention; or [0159] PBS as control.

[0160] All stress sessions were performed at the same time of the day (between 10 am and 12 pm) to minimize any influence of circadian rhythms. Stresses were performed using the wrap partial restrain stress model which is a mild non-ulcerogenic stressor (Williams, C. L et al.; Am. J. Physiol. 253, G582-586 (1987)).

[0161] Animals were lightly anesthetized (as previously described in Lee B. et al.; J. Neurogastroenterol. Motil. 17, 252-266 (2011), with ethyl-ether and their fore shoulders, upper forelimbs and thoracic trunk were draped in a confining harness of paper tape to restrict, but not to prevent, body movements.

[0162] Then rats were placed in their home cage for 2 h.

[0163] b. Rectal or Colorectal Distension and Colonic Hypersensitivity Measurement

[0164] Rats colonic sensitivity induced by PRS was assessed by quantifying electrical response through an electroencephalograph Reega Mini-hui (ALVAR, France) and expressed as number of abdominal cramps for a five min period (as previously described in Eutamene, H. et al.; J. Physiol. 506 (Pt 1), 245-252 (1998)).

[0165] Briefly, rats were accustomed to be in polypropylene tunnels (diameter 7 cm, length 20 cm) several days before colorectal distension (CRD) in order to minimize recording artifacts.

[0166] CRD was performed with an arterial embolectomy catheter (Fogarty; Edwards Laboratoire, Inc., Santa Ana, Calif., USA) introduced into the rectum (1 cm from the anus) and fixed at the base of the tail.

[0167] Distension of the colon was performed by connecting the catheter to a syringe and consecutive injections of different volumes (0.4, 0.8, 1.2 ml) with an interval of 5 minutes.

[0168] Each animal was recorded two days before the stress (basal measure) and just after the PRS (stress measure).

[0169] c. Statistical Analysis.

[0170] Statistical analysis was completed using GraphPad software (GraphPad Sofware, La Jolla, Calif., USA).

[0171] Results are presented with means.+-.SEM.

[0172] Differences in the number of abdominal cramps during 5 minutes to gradual CRD volumes were analyzed using a 2-way ANOVA (Treatment, Volume) followed by Bonferroni post-hoc test for multiple comparisons.

[0173] A p value of less than 0.05 was considered significant.

[0174] d. Results

[0175] To determine if F. prausnitzii CNCMI-4541 (outside of the invention) and CNCMI-4573 (according to the invention) strains are able to prevent acute stress generated symptoms on visceral sensitivity, both were tested on a model of Partial Restraint Stress (PRS).

[0176] PRS increased the number of abdominal cramps in response to CRD in a volume-dependent manner (FIGS. 3A and 3B).

[0177] In stressed rats treated with PBS, distensions at any volumes significantly increased the number of abdominal contractions compared to non-stressed animals (p<0.01) (FIGS. 3A and 3B).

[0178] F. prausnitzii CNCMI-4573 strain treatment prevented this stress-induced visceral hypersensitivity until 0.8 ml distension volume (p<0.001) (FIG. 3A).

[0179] In basal conditions, no difference was observed in the VMR to CRD between the different treatments (FIG. 3A).

[0180] In contrast, F. prausnitzii CNCMI-4541 strain did not show a protective effect (FIG. 3B).

[0181] In conclusion, it appears that a F. prausnitzii strain according to the invention prevents visceral hypersensitivity in a model of Partial Restraint Stress (PRS).

TABLE-US-00001 SEQ ID No: 1: 16S rRNA sequence of F. prausnitzii CNCM I-4540 AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGCGCCTAACA CATGCAAGTCGAACGAGTGAGAGAGAGCTTGCTTTCTCGAGCGAGTGGCGAAC GGGTGAGTAACGCGTGAGGAACCTGCCTCAAAGAGGGGGACAACAGTTGGAA ACGACTGCTAATACCGCATAAGCCCACGGCTCGGCATCGAGCAGAGGGAAAA GGAGTGATCCGCTTTGAGATGGCCTCGCGTCCGATTAGCTAGTTGGTGAGGTA ATGGCCCACCAAGGCGACGATCGGTAGCCGGACTGAGAGGTTGAACGGCCAC ATTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATA TTGCACAATGGGGGAAACCCTGATGCAGCGACGCCGCGTGGAGGAAGAAGGT CTTCGGATTGTAAACTCCTGTTGTTGGGGAAGATAATGACGGTACCCAACAAG GAAGTGACGGCTAACTACGTGCCAGCAGCCGCGGTAAAACGTAGGTCACAAG CGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGGAGACAAGTTG GAAGTGAAATCTATGGGCTCAACCCATAAACTGCTTTCAAAACTGTTTTTCTTG AGTAGTGCAGAGGTAGGCGGAATTCCCGGTGTAGCGGTGGAATGCGTAGATAT CGGGAGGAACACCAGTGGCGAAGGCGGCCTACTGGGCACCAACTGACGCTGA GGCTCGAAAGTGTGGGTAGCAAACAGGATTAGATACCCTGGTAGTCCACACCG TAAACGATGATTACTAGGTGTTGGAGGATTGACCCCTTCAGTGCCGCAGTTAA CACAATAAGTAATCCACCTGGGGAGTACGACCGCAAGGTTGAAACTCAAAGG AATTGACGGGGGCCCGCACAAGCAGTGGAGTATGTGGTTTAATTCGACGCAAC GCGAAGAACCTTACCAAGTCTTGACATCCCTTGACAGACATAGAAATATGTAT TCTCTTCGGAGCAAGGAGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGT GAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATGGTCAGTTACTAC GCAAGAGGACTCTGGCCAGACTGCCGTTGACAAAACGGAGGAAGGTGGGGAT GACGTCAAATCATCATGCCCTTTATGACTTGGGCTACACACGTACTACAATGGC GTTAAACAAAGAGAAGCAAGACCGCGAGGTGGAGCAAAACTCAGAAACAACG TCCCAGTTCGGACTGCAGGCTGCAACTCGCCTGCACGAAGTCGGAATTGCTAG TAATCGTGGATCAGCATGCCACGGTGAATACGTTCCCGGGCCTTGTACACACC GCCCGTCACACCATGAGAGCCGGGGGGACCCGAAGTCGGTAGTCTAACCGCA AGGAGGACGCCGCCGAAGGTAAAACTGGTGATTGGGGTGAAGTCGTAACAAG GTAGCCGT SEQ ID No: 2: 16S rRNA sequence of F. prausnitzii CNCM I-4542 AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGCGCCTAACA CATGCAAGTCGAACGAGCGAGAGAGAGCTTGCTTTCTCGAGCGAGTGGCGAAC GGGTGAGTAACGCGTGAGGAACCTGCCTCAAAGAGGGGGACAACAGTTGGAA ACGACTGCTAATACCGCATAAGCCCACGGGTCGGCATCGACCAGAGGGAAAA GGAGCAATCCGCTTTGAGATGGCCTCGCGTCCGATTAGCTAGTTGGTGAGGTA ATGGCCCACCAAGGCAACGATCGGTAGCCGGACTGAGAGGTTGAACGGCCAC ATTGGGACTGAGACGCGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATA TTGCACAATGGGGGAAACCCTGATGCAGCGACGCCGCGTGGAGGAAGAAGGT CTTCGGATTGTAAACTCCTGTTGTTGGGGAAGATAATGACGGTACCCAACAAG GAAGTGACGGCTAACTACGTGCCAGCAGCCGCGGTAAAACGTAGGTCACAAG CGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGAAGACAAGTTG GAAGTGAAATCTATGGGCTCAACCCATAAACTGCTTTCAAAACTGTTTTTCTTG AGTAGTGCAGAGGTAGGCGGAATTCCCGGTGTAGCGGTGGAATGCGTAGATAT CGGGAGGAACACCAGTGGCGAAGGCGGCCTACTGGGCACCAACTGACGCTGA GGCTCGAAAGTGTGGGTAGCAAACAGGATTAGATACCCTGGTAGTCCACACCG TAAACGATGGTTACTAGGTGTTGGAGGATTGACCCCTTCAGTGCCGCAGTTAA CACAATAAGTAATCCACCTGGGGAGTACGACCGCAAGGTTGAAACTCAAAGG AATTGACGGGGGCCCGCACAAGCAGTGGAGTATGTGGTTTAATTCGACGCAAC GCGAAGAACCTTACCAAGTCTTGACATCCCTTGACAGACATAGAAATATGTAA TCTCTTCGGAGCAAGGAGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGT GAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATGGTCAGTTACTAC GCAAGAGGACTCTGGCCAGACTGCCGTTGACAAAACGGAGGAAGGTGGGGAT GACGTCAAATCATCATGCCCTTTATGACTTGGGCTACACACGTACTACAATGGC GTTAAACAAAGAGAAGCAAGACCGCGAGGTGGAGCAAAACTCAGAAACAACG TCCCAGTTCGGACTGCAGGCTGCAACTCGCCTGCACGAAGTCGGAATTGCTAG TAATCGTGGATCAGCATGCCACGGTGAATACGTTCCCGGGCCTTGTACACACC GCCCGTCACACCATGAGAGCCGGGGGGACCCGAAGTCGGTAGTCTAACCGCA AGGAGGACGCCGCCGAAGGTAAAACTGGTGATTGGGGTGAAGTCGTAACAAG GTAGCCGT SEQ ID No: 3: 16S rRNA sequence of F. prausnitzii S3C12 GCGAGAGAGAGCTTGCTTTCTCGAGCGAGTGGCGAACGGGTGAGTA ACGCGTGAGGAACCTGCCTCAAAGAGGGGGACAACAGTTGGAAACGACTGCT AATACCGCATAAGCCCACGGCCCGGCATCGGGCAGAGGGAAAAGGAGCAATC CGCTTTGAGATGGCCTCGCGTCCGATTAGCTAGTTGGTGAGGTAACGGCCCAC CAAGGCGACGATCGGTAGCCGGACTGAGAGGTTGAACGGCCACATTGGGACT GAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAAT GGGGGAAACCCTGATGCAGCGACGCCGCGTGGAGGAAGAAGGTCTTCGGATT GTAAACTCCTGTTGTTGAGGAAGATAATGACGGTACTCAACAAGGAAGTGACG GCTAACTACGTGCCAGCAGCCGCGGTAAAACGTAGGTCACAAGCGTTGTCCGG AATTACTGGGTGTAAAGGGAGCGCAGGCGGGAAGACAAGTTGGAAGTGAAAT CCATGGGCTCAACCCATGAACTGCTTTCAAAACTGTTTTTCTTGAGTAGTGCAG AGGTAGGCGGAATTCCCGGTGTAGCGGTGGAATGCGTAGATATCGGGAGGAA CACCAGTGGCGAAGGCGGCCTACTGGGCACCAACTGACGCTGAGGCTCGAAA GTGTGGGTAGCAAACAGGATTAGATACCCTGGTAGTCCACACCGTAAACGATG ATTACTAGGTGTTGGAGGATTGACCCCTTCAGTGCCGCAGTTAACACAATAAG TAATCCACCTGGGGAGTACGACCGCAAGGTTGAAACTCAAAGGAATTGACGG GGGCCCGCACAAGCAGTGGAGTATGTGGTTTAATTCGACGCAACGCGAAGAAC CTTACCAAGTCTTGACATCCTGCGACGGTTCTGGAAACAGAACTTTCCTTCGGG ACGCAGAGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGG GTTAAGTCCCGCAACGAGCGCAACCCTTATGGTCAGTTACTACGCAAGAGGAC TCTGGCCAGACTGCCGTTGACAAAACGGAGGAAGGTGGGGATGACGTCAAAT CATCATGCCCTTTATGACTTGGGCTACACACGTACTACAATGGCGTTAAACAA AGAGAAGCAAGACCGCGAGGTGGAGCAAAACTCAGAAACAACGTCCCAGTTC GGACTGCAGGCTGCAACTCGCCTGCACGAAGTCGGAATTGCTAGTAATCGTGG ATCAGCATGCCACGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCAC ACCATGAGAGCCGGGGGGACCCGAAGTCGGTAGTCTAACCGCAAGGAGGACG CCGCCGAAGGTAAAACTGGTGATTGGGGTGAAGTCGTAACAAGGTAG SEQ ID No: 4: 16S rRNA sequence of F. prausnitzii S3G1 AGCGAGAGAGAGCTTGCTTTCTCGAGCGAGTGGCGAACGGGTGAGT AACGCGTGAGGAACCTGCCTCAAAGAGGGGGACAACAGTTGGAAACGACTGC TAATACCGCATAAGCCCACGGTGCCGCATGGCACAGAGGGAAAAGGAGCAAT CCGCTTTGAGATGGCCTCGCGTCCGATTAGCTAGTTGGTGAGGTAACGGCCCA CCAAGGCGACGATCGGTAGCCGGACTGAGAGGTTGAACGGCCACATTGGGAC TGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA TGGGGGAAACCCTGATGCAGCGACGCCGCGTGGAGGAAGAAGGTCTTCGGAT TGTAAACTCCTGTTGTTGGGGAAGATAATGACGGTACCCAACAAGGAAGTGAC GGCTAACTACGTGCCAGCAGCCGCGGTAAAACGTAGGTCACAAGCGTTGTCCG GAATTACTGGGTGTAAAGGGAGCGCAGGCGGGAAGACAAGTTGGAAGTGAAA TCTATGGGCTCAACCCATAAACTGCTTTCAAAACTGTTTTTCTTGAGTAGTGCA GAGGTAGGCGGAATTCCCGGTGTAGCGGTGGAATGCGTAGATATCGGGAGGA ACACCAGTGGCGAAGGCGGCCTACTGGGCACCAACTGACGCTGAGGCTCGAA AGTGTGGGTAGCAAACAGGATTAGATACCCTGGTAGTCCACACCGTAAACGAT GATTACTAGGTGTTGGAGGATTGACCCCTTCAGTGCCGCAGTTAACACAATAA GTAATCCACCTGGGGAGTACGACCGCAAGGTTGAAACTCAAAGGAATTGACG GGGGCCCGCACAAGCAGTGGAGTATGTGGTTTAATTCGACGCAACGCGAAGA ACCTTACCAAGTCTTGACATCCCTTGACAGGCATAGAAATATGTTTTCTCTTCG GAGCAAGGAGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTT GGGTTAAGTCCCGCAACGAGCGCAACCCTTATGGTCAGTTACTACGCAAGAGG ACTCTGGCCAGACTGCCGTTGACAAAACGGAGGAAGGTGGGGATGACGTCAA ATCATCATGCCCTTTATGACTTGGGCTACACACGTACTACAATGGCGTTAAACA AAGAGAAGCAAGACCGCGAGGTGGAGCAAAACTCAGAAACAACGTCCCAGTT CGGACTGCAGGCTGCAACTCGCCTGCACGAAGTCGGAATTGCTAGTAATCGTG GATCAGCATGCCACGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCA CACCATGAGAGCCGGGGGGACCCGAAGTCGGTAGTCTAACCGCAAGGAGGAC GCCGCCGAAGGTAAAACTGGTGATTGGGGTGAAGTCGTAACAAG SEQ ID No: 5: 16S rRNA sequence of F. prausnitzii CNCM I-4574 CGAGTGGCGAACGGGTGAGTAACGCGTGAGGAACCTGCCTCAAAG AGGGGGACAACAGTTGGAAACGACTGCTAATACCGCATAAGCCCACAGGTCG GCATCGACCAGAGGGAAAAGGAGCAATCCGCTTTGAGATGGCCTCGCGTCCGA TTAGCTAGTTGGTGAGGTAATGGCCCACCAAGGCAACGATCGGTAGCCGGACT GAGAGGTTGAACGGCCACATTGGGACTGAGACACGGCCCAGACTCCTACGGG AGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGATGCAGCGACGC CGCGTGGAGGAAGAAGGTCTTCGGATTGTAAACTCCTGTTGTTGAGGAAGATA ATGACGGTACTCAACAAGGAAGTGACGGCTAACTACGTGCCAGCAGCCGCGGT AAAACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCA GGCGGGAAGACAAGTTGGAAGTGAAATCTATGGGCTCAACCCATAAACTGCTT TCAAAACTGTTTTTCTTGAGTAGTGCAGAGGTAGGCGGAATTCCCGGTGTAGC

GGTGGAATGCGTAGATATCGGGAGGAACACCAGTGGCGAAGGCGGCCTACTG GGCACCAACTGACGCTGAGGCTCGAAAGTGTGGGTAGCAAACAGGATTAGAT ACCCTGGTAGTCCACACCGTAAACGATGATTACTAGGTGTTGGAGGATTGACC CCTTCAGTGCCGCAGTTAACACAATAAGTAATCCACCTGGGGAGTACGACCGC AAGGTTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCAGTGGAGTATG TGGTTTAATTCGACGCAACGCGAAGAACCTTACCAAGTCTTGACATCCTGTGA CGATGCTGGAAACATGTTTTTCCTTCGGAACGCAGAGACAGGTGGTGCATGGT TGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAAC CCTTACTGTCAGTTACTACGCAAGAGGACTCTGGCAGGACTGCCGTTGACAAA ACGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCTTTATGACTTGGGCT ACACACGTACTACAATGGCGTTAAACAAAGAGAAGCAAGACCGCGAGGTGGA GCAAAACTCAGAAACAACGTCCCAGTTCGGACTGCAGGCTGCAACTCGCCTGC ACGAAGTCGGAATTGCTAGTAATCGTGGATCAGCATGCCACGGTGAATACGTT CCCGGGCCTTGTACACACCGCCCGTCACACCATGAGAGCCGGGGGGACCCGAA GTCGGTAGTCTAACCGCAAGGAGGACGCCGCCGAAGGTAAAACTGGTGATTG GGGTGAAGTCGTAACAAGGTAGCC SEQ ID No: 6: 16S rRNA sequence of F. prausnitzii CNCM I-4543 CGAGCGAGAGAGAGCTTGCTTTCTCAATCGAGTGGCGAACGGGTGA GTAACGCGTGAGGAACCTGCCTCAAAGAGGGGGACAACAGTTGGAAACGACT GCTAATACCGCATAAGCCCACAGGTCGGCATCGACCAGAGGGAAAAGGAGCA ATCCGCTTTGAGATGGCCTCGCGTCCGATTAGCTAGTTGGTGAGGTAATGGCCC ACCAAGGCAACGATCGGTAGCCGGACTGAGAGGTTGAACGGCCACATTGGGA CTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACA ATGGGGGAAACCCTGATGCAGCGACGCCGCGTGGAGGAAGAAGGTCTTCGGA TTGTAAACTCCTGTTGTTGAGGAAGATAATGACGGTACTCAACAAGGAAGTGA CGGCTAACTACGTGCCAGCAGCCGCGGTAAAACGTAGGTCACAAGCGTTGTCC GGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGAAGACAAGTTGGAAGTGAA ATCTATGGGCTCAACCCATAAACTGCTTTCAAAACTGTTTTTCTTGAGTAGTGC AGAGGTAGGCGGAATTCCCGGTGTAGCGGTGGAATGCGTAGATATCGGGAGG AACACCAGTGGCGAAGGCGGCCTACTGGGCACCAACTGACGCTGAGGCTCGA AAGTGTGGGTAGCAAACAGGATTAGATACCCTGGTAGTCCACACCGTAAACGA TGATTACTAGGTGTTGGAGGATTGACCCCTTCAGTGCCGCAGTTAACACAATA AGTAATCCACCTGGGGAGTACGACCGCAAGGTTGAAACTCAAAGGAATTGAC GGGGGCCCGCACAAGCAGTGGAGTATGTGGTTTAATTCGACGCAACGCGAAG AACCTTACCAAGTCTTGACATCCTGTGACGAACCTGGAAATATGTTTTTCCTTC GGAACGCAGAGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGT TGGGTTAAGTCCCGCAACGAGCGCAACCCTTACTGTCAGTTACTACGCAAGAG GACTCTGGCAGGACTGCCGTTGACAAAACGGAGGAAGGTGGGGATGACGTCA AATCATCATGCCCTTTATGACTTGGGCTACACACGTACTACAATGGCGTTAAAC AAAGAGAAGCAAGACCGCGAGGTGGAGCAAAACTCAGAAACAACGTCCCAGT TCGGACTGCAGGCTGCAACTCGCCTGCACGAAGTCGGAATTGCTAGTAATCGT GGATCAGCATGCCACGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTC ACACCATGAGAGCCGGGGGGACCCGAAGTCGGTAGTCTAACCGCAAGGAGGA CGCCGCCGAAGGTAAAACTGGTGATTGGGGTGAAGTCGTACAG SEQ ID No: 7: 16S rRNA sequence of F. prausnitzii S9D8 AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGCGCCTAACA CATGCAAGTCGAACGAGCGAGAGAGAGCTTGCTTTCTCGAGCGAGTGGCGAAC GGGTGAGTAACGCGTGGGGAACCTGCCTCAAAGAGGGGGACAACAGTTGGAA ACGACTGCTAATACCGCATAAGCCCACGACCTGGCATCGGGTTGAGGGAAAAG GAGCAATCCGCTTTGAGATGGCCTCGCGTCCGATTAGCTAGTTGGTGAGGTAA TGGCCCACCAAGGCAACGATCGGTAGCCGGACTGAGAGGTTGAACGGCCACA TTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATT GCACAATGGGGGAAACCCTGATGCAGCGACGCCGCGTGGAGGAAGAAGGTCT TCGGATTGTAAACTCCTGTTGTTGAGGAAGATAATGACGGTACTCAACAAGGA AGTGACGGCTAACTACGTGCCAGCAGCCGCGGTAAAACGTAGGTCACAAGCGT TGTCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGAAGACAAGTTGGAA GTGAAATCCATGGGCTCAACCCATGAACTGCTTTCAAAACTGTTTTTCTTGAGT AGTGCAGAGGTAGGCGGAATTCCCGGTGTAGCGGTGGAATGCGTAGATATCGG GAGGAACACCAGTGGCGAAGGCGGCCTACTGGGCACCAACTGACGCTGAGGC TCGAAAGTGTGGGTAGCAAACAGGATTAGATACCCTGGTAGTCCACACCGTAA ACGATGATTACTAGGTGTTGGAGGATTGACCCCTTCAGTGCCGCAGTTAACAC AATAAGTAATCCACCTGGGGAGTACGACCGCAAGGTTGAAACTCAAAGGAATT GACGGGGGCCCGCACAAGCAGTGGAGTATGTGGTTTAATTCGACGCAACGCGA AGAACCTTACCAAGTCTTGACATCCTGCGACGGTGCTGGAAACAGTGCTTTCCT TCGGGACGCAGAGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGAT GTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATGGTCAGTTACTACGCAAG AGGACTCTGGCCAGACTGCCGTTGACAAAACGGAGGAAGGTGGGGATGACGT CAAATCATCATGCCCTTTATGACTTGGGCTACACACGTACTACAATGGCGTTAA ACAAAGAGAAGCAAGACCGCGAGGTGGAGCAAAACTCAGAAACAACGTCCCA GTTCGGACTGCAGGCTGCAACTCGCCTGCACGAAGTCGGAATTGCTAGTAATC GTGGATCAGCATGCCACGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCG TCACACCATGAGAGCCGGGGGGACCCGAAGTCGGTAGTCTAACCGCAAGGAG GACGCCGCCGAAGGTAAAACTGGTGATTGGGGTGAAGTCGTAACAAGGTAGC CGT SEQ ID No: 8: 16S rRNA sequence of F. prausnitzii S9G3 GCATGCTCGAGCGGCCGCCAGTGTGATGGATATCTGCAGAATTCGC CCTTAGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGCGCCTAACACAT GCAAGTCGAACGAGCGAGAGAGAGCTTGCTTTCTCGAGCGAGTGGCGAACGG GTGAGTAACGCGTGAGGAACCTGCCTCAAAGAGGGGGACAACAGTTGGAAAC GACTGCTAATACCGCATAAGCCCACGACCCGGCATCGGGTAGAGGGAAAAGG AGCAATCCGCTTTGAGATGGCCTCGCGTCCGATTAGCTAGTTGGTGAGGTAAC GGCCCACCAAGGCGACGATCGGTAGCCGGACTGAGAGGTTGAACGGCCACAT TGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATT GCACAATGGGGGAAACCCTGATGCAGCGACGCCGCGTGGAGGAAGAAGGTCT TCGGATTGTAAACTCCTGTTGTTGAGGAAGATAATGACGGTACTCAACAAGGA AGTGACGGCTAACTACGTGCCAGCAGCCGCGGTAAAACGTAGGTCACAAGCGT TGTCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGAAGACAAGTTGGAA GTGAAATCCATGGGCTCAACCCATGAACTGCTTTCAAAACTGTTTTTCTTGAGT AGTGCAGAGGTAGGCGGAATTCCCGGTGTAGCGGTGGAATGCGTAGATATCGG GAGGAACACCAGTGGCGAAGGCGGCCTACTGGGCACCAACTGACGCTGAGGC TCGAAAGTGTGGGTAGCAAACAGGATTAGATACCCTGGTAGTCCACACCGTAA ACGATGATTACTAGGTGTTGGAGGATTGACCCCTTCAGTGCCGCAGTTAACAC AATAAGTAATCCACCTGGGGAGTACGACCGCAAGGTTGAAACTCAAAGGAATT GACGGGGGCCCGCACAAGCAGTGGAGTATGTGGTTTAATTCGACGCAACGCGA AGAACCTTACCAAGTCTTGACATCCTGCGACGGTTCTGGAAACAGAACTTTCCT TCGGGACGCAGAGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGAT GTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATGGTCAGTTACTACGCAAG AGGACTCTGGCCAGACTGCCGTTGACAAAACGGAGGAAGGTGGGGATGACGT CAAATCATCATGCCCTTTATGACTTGGGCTACACACGTACTACAATGGCGTTAA ACAAAGAGAAGCAAGACCGCGAGGTGGAGCAAAACTCAGAAACAACGTCCCA GTTCGGACTGCAGGCTGCAACTCGCCTGCACGAAGTCGGAATTGCTAGTAATC GTGGATCAGCATGCCACGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCG TCACACCATGAGAGCCGGGGGGACCCGAAGTCGGTAGTCTAACCGCAAGGAG GACGCCGCCGAAGGTAAAACTGGTGATTGGGGTGAAGTCGTAACAAGGTAGC CGTAAGGGCGAA SEQ ID No: 9: 16S rRNA sequence of F. prausnitzii CNCM I-4644 GCATCGGGCAGAGGGAAAAGGAGCAATCCGCTTTGAGATGGCCTC GCGTCCGATTAGCTAGTTGGTGAGGTAATGGCCCACCAAGGCGACGATCGGTA GCCGGACTGAGAGGTTGAACGGCCACATTGGGACTGAGACACGGCCCAGACT CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGATGC AGCGACGCCGCGTGGAGGAAGAAGGTCTTCGGATTGTAAACTCCTGTTGTTGA GGAAGATAATGACGGTACTCAACAAGGAAGTGACGGCTAACTACGTGCCAGC AGCCGCGGTAAAACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAG GGAGCGCAGGCGGGAAGACAAGTTGGAAGTGAAATCCATGGGCTCAACCCAT GAACTGCTTTCAAAACTGTTTTTCTTGAGTAGTGCAGAGGTAGGCGGAATTCCC GGTGTAGCGGTGGAATGCGTAGATATCGGGAGGAACACCAGTGGCGAAGGCG GCCTACTGGGCACCAACTGACGCTGAGGCTCGAAAGTGTGGGTAGCAAACAG GATTAGATACCCTGGTAGTCCACACTGTAAACGATGATTACTAGGTGTTGGAG GATTGACCCCTTCAGTGCCGCAGTTAACACAATAAGTAATCCACCTGGGGAGT ACGACCGCAAGGTTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCAGT GGAGTATGTGGTTTAATTCGACGCAACGCGAAGAACCTTACCAAGTCTTGACA TCCTGCGACGCACATAGAAATATGTGTTTCCTTCGGGACGCAGAGACAGGTGG TGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGA GCGCAACCCTTATGGTCAGTTACTACGCAAGAGGACTCTGGCCAGACTGCCGT TGACAAAACGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCTTTATGAC TTGGGCTACACACGTACTACAATGGCGTTAAACAAAGAGAAGCAAGACCGCG AGGTGGAGCAAAACTCAGAAACAACGTCCCAGTTCGGACTGCAGGCTGCAACT

CGCCTGCACGAAGTCGGAATTGCTAGTAATCGCAGATCAGCATGCTGCGGTGA ATACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCATGAGAGCCGGGGGG ACCCGAAGTCGGTAGTCTAACCGCAAGGAGGACGCCGCCGAAGGTAAAACTG GTGATTGGGGTGAAGTCGTAACAAGGTAG SEQ ID No: 10: 16S rRNA sequence of F. prausnitzii CNCM I-4544 CGAGCGAGAGAGAGCTTGCTTTCTCGAGCGAGTGGCGAACGGGTGA GTAACGCGTGAGGAACCTGCCTCAAAGAGGGGGACAACAGTTGGAAACGACT GCTAATACCGCATAAGCCCACGGGTCGGCATCGACCAGAGGGAAAAGGAGCA ATCCGCTTTGAGATGGCCTCGCGTCCGATTAGCTAGTTGGTGAGGTAACGGCC CACCAAGGCAACGATCGGTAGCCGGACTGAGAGGTTGAACGGCCACATTGGG ACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCAC AATGGGGGAAACCCTGATGCAGCGACGCCGCGTGGAGGAAGAAGGTCTTCGG ATTGTAAACTCCTGTTGTTGGGGAAGATAATGACGGTACCCAACAAGGAAGTG ACGGCTAACTACGTGCCAGCAGCCGCGGTAAAACGTAGGTCACAAGCGTTGTC CGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGAAGACAAGTTGGAAGTGA AATCTATGGGCTCAACCCATAAACTGCTTTCAAAACTGTTTTTCTTGAGTAGTG CAGAGGTAGGCGGAATTCCCGGTGTAGCGGTGGAATGCGTAGATATCGGGAG GAACACCAGTGGCGAAGGCGGCCTACTGGGCACCAACTGACGCTGAGGCTCG AAAGTGTGGGTAGCAAACAGGATTAGATACCCTGGTAGTCCACACCGTAAACG ATGATTACTAGGTGTTGGAGGATTGACCCCTTCAGTGCCGCAGTTAACACAAT AAGTAATCCACCTGGGGAGTACGACCGCAAGGTTGAAACTCAAAGGAATTGA CGGGGGCCCGCACAAGCAGTGGAGTATGTGGTTTAATTCGACGCAACGCGAAG AACCTTACCAAGTCTTGACATCCCTTGACAGACATAGAAATATGTAATCTCTTC GGAGCAAGGAGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGT TGGGTTAAGTCCCGCAACGAGCGCAACCCTTATGGTCAGTTACTACGCAAGAG GACTCTGGCCAGACTGCCGTTGACAAAACGGAGGAAGGTGGGGATGACGTCA AATCATCATGCCCTTTATGACTTGGGCTACACACGTACTACAATGGCGTTAAAC AAAGAGAAGCAAGACCGCGAGGTGGAGCAAAACTCAGAAACAACGTCCCAGT TCGGACTGCAGGCTGCAACTCGCCTGCACGAAGTCGGAATTGCTAGTAATCGT GGATCAGCATGCCACGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTC ACACCATGAGAGCCGGGGGGACCCGAAGTCGGTAGTCTAACCGCAAGGAGGA CGCCGCCGAAGGTAAAACTGGTGATTGGGGTGAAGTCGTAACAAG SEQ ID No: 11: 16S rRNA sequence of F. prausnitzii S10H3 GAGCGAGTGGCGAACGGGTGAGTAACGCGTGAGGAACCTGCCTCA AAGAGGGGGACAACAGTTGGAAACGACTGCTAATACCGCATAAGCCCACGAC CCGGCATCGGGTAGAGGGAAAAGGAGCAATCCGCTTTGAGATGGCCTCGCGTC CGATTAGCTAGTTGGTGAGGTAATGGCCCACCAAGGCGACGATCGGTAGCCGG ACTGAGAGGTTGAACGGCCACATTGGGACTGAGACACGGCCCAGACTCCTACG GGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGATGCAGCGAC GCCGCGTGGAGGAAGAAGGTCTTCGGATTGTAAACTCCTGTTGTTGAGGAAGA TAATGACGGTACTCAACAAGGAAGTGACGGCTAACTACGTGCCAGCAGCCGCG GTAAAACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGC AGGCGGGAAGACAAGTTGGAAGTGAAATCCATGGGCTCAACCCATGAACTGC TTTCAAAACTGTTTTTCTTGAGTAGTGCAGAGGTAGGCGGAATTCCCGGTGTAG CGGTGGAATGCGTAGATATCGGGAGGAACACCAGTGGCGAAGGCGGCCTACT GGGCACCAACTGACGCTGAGGCTCGAAAGTGTGGGTAGCAAACAGGATTAGA TACCCTGGTAGTCCACACTGTAAACGATGATTACTAGGTGTTGGAGGATTGAC CCCTTCAGTGCCGCAGTTAACACAATAAGTAATCCACCTGGGGAGTACGACCG CAAGGTTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCAGTGGAGTAT GTGGTTTAATTCGACGCAACGCGAAGAACCTTACCAAGTCTTGACATCCTGCG ACGCACATAGAAATATGTGTTTCCTTCGGGACGCAGAGACAGGTGGTGCATGG TTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAA CCCTTATGGTCAGTTACTACGCAAGAGGACTCTGGCCAGACTGCCGTTGACAA AACGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCTTTATGACTTGGGC TACACACGTACTACAATGGCGTTAAACAAAGAGAAGCAAGACCGCGAGGTGG AGCAAAACTCAGAAACAACGTCCCAGTTCGGACTGCAGGCTGCAACTCGCCTG CACGAAGTCGGAATTGCTAGTAATCGCAGATCAGCATGCTGCGGTGAATACGT TCCCGGGCCTTGTACACACCGCCCGTCACACCATGAGAGCCGGGGGGACCCGA AGTCGGTAGTCTAACCGCAAGGAGGACGCCGCCGAAGGTAAAACTGGTGATT GGGGTGAAGTCGTAACAAG SEQ ID No: 12: 16S rRNA sequence of F. prausnitzii S13A7 AGCTTGCTTTCTCGAGCGAGTGGCGAACGGGTGAGTAACGCGTGAG GAACCTGCCTCAAAGAGGGGGACAACAGTTGGAAACGACTGCTAATACCGCA TAAGCCCACGGGTCGGCATCGACCAGAGGGAAAAGGAGCAATCCGCTTTGAG ATGGCCTCGCGTCCGATTAGCTAGTTGGTGAGGTAACGGCCCACCAAGGCGAC GATCGGTAGCCGGACTGAGAGGTTGAACGGCCACATTGGGACTGAGACACGG CCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAAC CCTGATGCAGCGACGCCGCGTGGAGGAAGAAGGTCTTCGGATTGTAAACTCCT GTTGTTGGGGAAGATAATGACGGTACCCAACAAGGAAGTGACGGCTAACTAC GTGCCAGCAGCCGCGGTAAAACGTAGGTCACAAGCGTTGTCCGGAATTACTGG GTGTAAAGGGAGCGCAGGCGGGAAGACAAGTTGGAAGTGAAATCTATGGGCT CAACCCATAAACTGCTTTCAAAACTGTTTTTCTTGAGTAGTGCAGAGGTAGGCG GAATTCCCGGTGTAGCGGTGGAATGCGTAGATATCGGGAGGAACACCAGTGGC GAAGGCGGCCTACTGGGCACCAACTGACGCTGAGGCTCGAAAGTGTGGGTAG CAAACAGGATTAGATACCCTGGTAGTCCACACCGTAAACGATGATTACTAGGT GTTGGAGGATTGACCCCTTCAGTGCCGCAGTTAACACAATAAGTAATCCACCT GGGGAGTACGACCGCAAGGTTGAAACTCAAAGGAATTGACGGGGGCCCGCAC AAGCAGTGGAGTATGTGGTTTAATTCGACGCAACGCGAAGAACCTTACCAAGT CTTGACATCCCTTGACAGACATAGAAATATGTATTCTCTTCGGAGCAAGGAGA CAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCC GCAACGAGCGCAACCCTTATGGTCAGTTACTACGCAAGAGGACTCTGGCCAGA CTGCCGTTGACAAAACGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCT TTATGACTTGGGCTACACACGTACTACAATGGCGTTAAACAAAGAGAAGCAAG ACCGCGAGGTGGAGCAAAACTCAGAAACAACGTCCCAGTTCGGACTGCAGGC TGCAACTCGCCTGCACGAAGTCGGAATTGCTAGTAATCGTGGATCAGCATGCC ACGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACACCATGAGAGC CGGGGGGACCCGAAGTCGGTAGTCTAACCGCAAGGAGGACGCCGCCGAAGGT AAAACTGGTGATTGGGGTGAAGTCGTAACAAG SEQ ID No: 13: 16S rRNA sequence of F. prausnitzii CNCM I-4575 ATCGAGTGGCGAACGGGTGAGTAACGCGTGAGGAACCTGCCTCAA AGAGGGGGACAACAGTTGGAAACGACTGCTAATACCGCATAAGCCCACGGCT CGGCATCGAGCAGAGGGAAAAGGAGCAATCCGCTTTGAGATGGCCTCGCGTCC GATTAGCTAGTTGGTGAGGTAATGGCCCACCAAGGCGACGATCGGTAGCCGGA CTGAGAGGTTGAACGGCCACATTGGGACTGAGACACGGCCCAGACTCCTACGG GAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGATGCAGCGACG CCGCGTGGAGGAAGAAGGTCTTCGGATTGTAAACTCCTGTTGTTGAGGAAGAT AATGACGGTACTCAACAAGGAAGTGACGGCTAACTACGTGCCAGCAGCCGCG GTAAAACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGC AGGCGGGCGATCAAGTTGGAAGTGAAATCCATGGGCTCAACCCATGAACTGCT TTCAAAACTGATTGTCTTGAGTAGTGCAGAGGTAGGCGGAATTCCCGGTGTAG CGGTGGAATGCGTAGATATCGGGAGGAACACCAGTGGCGAAGGCGGCCTACT GGGCACCAACTGACGCTGAGGCTCGAAAGTGTGGGTAGCAAACAGGATTAGA TACCCTGGTAGTCCACACCGTAAACGATGATTACTAGGTGTTGGAGGATTGAC CCCTTCAGTGCCGCAGTTAACACAATAAGTAATCCACCTGGGGAGTACGACCG CAAGGTTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCAGTGGAGTAT GTGGTTTAATTCGACGCAACGCGAAGAACCTTACCAAGTCTTGACATCCTGCG ACGATGCTAGAAATAGTATTTTCCTTCGGGACGCAGAGACAGGTGGTGCATGG TTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAA CCCTTATGGTCAGTTACTACGCAAGAGGACTCTGGCCAGACTGCCGTTGACAA AACGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCTTTATGACTTGGGC TACACACGTACTACAATGGCGTTAAACAAAGAGAAGCAAGACCGCGAGGTGG AGCAAAACTCAGAAACAACGTCCCAGTTCGGACTGCAGGCTGCAACTCGCCTG CACGAAGTCGGAATTGCTAGTAATCGCAGATCAGCATGCTGCGGTGAATACGT TCCCGGGCCTTGTACACACCGCCCGTCACACCATGAGAGCCGGGGGGACCCGA AGTCGGTAGTCTAACCGCAAGGAGGACGCCGCCGAAGGTAAAACTGGTGATT GGGGTGAAGTCGTAACAAGGTAGCCGTC SEQ ID No: 14: 16S rRNA sequence of F. prausnitzii CNCM I-4573 GAGAGAGCTTGCTTTCTCAAGCGAGTGGCGAACGGGTGAGTAACGC GTGAGGAACCTGCCTCAAAGAGGGGGACAACAGTTGGAAACGACTGCTAATA CCGCATAAGCCCACGACCCGGCATCGGGTAGAGGGAAAAGGAGCAATCCGCT TTGAGATGGCCTCGCGTCCGATTAGCTAGTTGGTGAGGTAACGGCCCACCAAG GCGACGATCGGTAGCCGGACTGAGAGGTTGAACGGCCACATTGGGACTGAGA CACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGG GAAACCCTGATGCAGCGACGCCGCGTGGAGGAAGAAGGTCTTCGGATTGTAA ACTCCTGTTGTTGAGGAAGATAATGACGGTACTCAACAAGGAAGTGACGGCTA ACTACGTGCCAGCAGCCGCGGTAAAACGTAGGTCACAAGCGTTGTCCGGAATT ACTGGGTGTAAAGGGAGCGCAGGCGGGAAGACAAGTTGGAAGTGAAATCCAT GGGCTCAACCCATGAACTGCTTTCAAAACTGTTTTTCTTGAGTAGTGCAGAGGT

AGGCGGAATTCCCGGTGTAGCGGTGGAATGCGTAGATATCGGGAGGAACACC AGTGGCGAAGGCGGCCTACTGGGCACCAACTGACGCTGAGGCTCGAAAGTGT GGGTAGCAAACAGGATTAGATACCCTGGTAGTCCACACTGTAAACGATGATTA CTAGGTGTTGGAGGATTGACCCCTTCAGTGCCGCAGTTAACACAATAAGTAAT CCACCTGGGGAGTACGACCGCAAGGTTGAAACTCAAAGGAATTGACGGGGGC CCGCACAAGCAGTGGAGTATGTGGTTTAATTCGACGCAACGCGAAGAACCTTA CCAAGTCTTGACATCCTGCGACGGTGCTGGAAACAGTGCTTTCCTTCGGGACG CAGAGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTT AAGTCCCGCAACGAGCGCAACCCTTATGGTCAGTTACTACGCAAGAGGACTCT GGCCAGACTGCCGTTGACAAAACGGAGGAAGGTGGGGATGACGTCAAATCAT CATGCCCTTTATGACTTGGGCTACACACGTACTACAATGGCGTTAAACAAAGA GAAGCAAGACCGCGAGGTGGAGCAAAACTCAGAAACAACGTCCCAGTTCGGA CTGCAGGCTGCAACTCGCCTGCACGAAGTCGGAATTGCTAGTAATCGCAGATC AGCATGCTGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACACC ATGAGAGCCGGGGGGACCCGAAGTCGGTAGTCTAACCGCAAGGAGGACGCCG CCGAAGGTAAAACTGGTGATTGGGGTGAAGTCGTAACAAG SEQ ID No: 15: 16S rRNA sequence of F. prausnitzii CNCM I-4546 CAAGCGAGTGGCGAACGGGTGAGTAACGCGTGAGGAACCTGCCTC AAAGAGGGGGACAACAGTTGGAAACGACTGCTAATACCGCATAAGCCCACGA CCCGGCATCGGGTAGAGGGAAAAGGAGCAATCCGCTTTGAGATGGCCTCGCGT CCGATTAGCTAGTTGGTGAGGTAACGGCCCACCAAGGCGACGATCGGTAGCCG GACTGAGAGGTTGAACGGCCACATTGGGACTGAGACACGGCCCAGACTCCTAC GGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGATGCAGCGA CGCCGCGTGGAGGAAGAAGGTCTTCGGATTGTAAACTCCTGTTGTTGAGGAAG ATAATGACGGTACTCAACAAGGAAGTGACGGCTAACTACGTGCCAGCAGCCGC GGTAAAACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCG CAGGCGGGAAGACAAGTTGGAAGTGAAATCCATGGGCTCAACCCATGAACTG CTTTCAAAACTGTTTTTCTTGAGTAGTGCAGAGGTAGGCGGAATTCCCGGTGTA GCGGTGGAATGCGTAGATATCGGGAGGAACACCAGTGGCGAAGGCGGCCTAC TGGGCACCAACTGACGCTGAGGCTCGAAAGTGTGGGTAGCAAACAGGATTAG ATACCCTGGTAGTCCACACTGTAAACGATGATTACTAGGTGTTGGAGGATTGA CCCCTTCAGTGCCGCAGTTAACACAATAAGTAATCCACCTGGGGAGTACGACC GCAAGGTTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCAGTGGAGTA TGTGGTTTAATTCGACGCAACGCGAAGAACCTTACCAAGTCTTGACATCCTGC GACGCACATAGAAATATGTGTTTCCTTCGGGACGCAGAGACAGGTGGTGCATG GTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCA ACCCTTATGGTCAGTTACTACGCAAGAGGACTCTGGCCAGACTGCCGTTGACA AAACGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCTTTATGACTTGGG CTACACACGTACTACAATGGCGTTAAACAAAGAGAAGCAAGACCGCGAGGTG GAGCAAAACTCAGAAACAACGTCCCAGTTCGGACTGCAGGCTGCAACTCGCCT GCACGAAGTCGGAATTGCTAGTAATCGCAGATCAGCATGCTGCGGTGAATACG TTCCCGGGCCTTGTACACACCGCCCGTCACACCATGAGAGCCGGGGGGACCCG AAGTCGGTAGTCTAACCGCAAGGAGGACGCCGCCGAAGGTAAAACTGGTGAT TGGGGTGAAGTCGTAACAAGGGTAG SEQ ID No: 16: 16S rRNA sequence of F. prausnitzii CNCM I-4541 AGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGCGCCTAACA CATGCAAGTCGAACGAGCGAGAGAGAGCTTGCTTTCTCGAGCGAGTGGCGAAC GGGTGAGTAACGCGTGAGGAACCTGCCTCAAAGAGGGGGACAACAGTTGGAA ACGACTGCTAATACCGCATAAGCCCACGACCCGGCATCGGGTTGAGGGAAAA GGAGCAATCCGCTTTGAGATGGCCTCGCGTCCGATTAGCTAGTTGGTGAGGTA ACGGCCCACCAAGGCGACGATCGGTAGCCGGACTGAGAGGTTGAACGGCCAC ATTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATA TTGCACAATGGGGGAAACCCTGATGCAGCGACGCCGCGTGGAGGAAGAAGGT CTTCGGATTGTAAACTCCTGTTGTTGGGGAAGATAATGACGGTACCCAACAAG GAAGTGACGGCTAACTACGTGCCAGCAGCCGCGGTAAAACGTAGGTCACAAG CGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCAGGCGGGAAGACAAGTTG GAAGTGAAATCCATGGGCTTAACCCATGAACTGCTTTCAAAACTGTTTTTCTTG AGTAGTGCAGAGGTAGGCGGAATTCCCGGTGTAGCGGTGGAATGCGTAGATAT CGGGAGGAACACCAGTGGCGAAGGCGGCCTACTGGGCACCAACTGACGCTGA GGCTCGAAAGTGTGGGTAGCAAACAGGATTAGATACCCTGGTAGTCCACACCG TAAACGATGATTACTAGGTGTTGGAGGATTGACCCCTTCAGTGCCGCAGTTAA CACAATAAGTAATCCACCTGGGGAGTACGACCGCAAGGTTGAAACTCAAAGG AATTGACGGGGGCCCGCACAAGCAGTGGAGTATGTGGTTTAATTCGACGCAAC GCGAAGAACCTTACCAAGTCTTGACATCCTGTGACAGACGTAGAAATACGTTC TTCCTTCGGGACACAGAGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGT GAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATGGTCAGTTACTAC GCAAGAGGACTCTGGCCAGACTGCCGTTGACAAAACGGAGGAAGGTGGGGAT GACGTCAAATCATCATGCCCTTTATGACTTGGGCTACACACGTACTACAATGGC GTTAAACAAAGAGAAGCAAGACCGCGAGGTGGAGCAAAACTCAGAAACAACG TCCCAGTTCGGACTGCAGGCTGCAACTCGCCTGCACGAAGTCGGAATTGCTAG TAATCGTGGATCAGCATGCCACGGTGAATACGTTCCCGGGCCTTGTACACACC GCCCGTCACACCATGAGAGCCGGGGGGACCCGAAGTCGGTAGTCTAACCGCA AGGAGGACGCCGCCGAAGGTAAAACTGGTGATTGGGGTGAAGTCGTAACAAG GTAGCCGT SEQ ID No: 17: 16S rRNA sequence of F. prausnitzii S13E3 TTAGTGGCGAACGGGTGAGTAACGCGTGAGTAACCTGCCCTGGAGT GGGGGACAACAGTTGGAAACGACTGCTAATACCGCATAAGCCCACGGCCCGG CATCGGGCTGCGGGAAAAGGATTTATTCGCTTCAGGATGGACTCGCGTCCAAT TAGCTAGTTGGTGAGGTAACGGCCCACCAAGGCGACGATTGGTAGCCGGACTG AGAGGTTGAACGGCCACATTGGGACTGAGACACGGCCCAGACTCCTACGGGA GGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGATGCAGCGACGCC GCGTGGAGGAAGAAGGTTTTCGGATTGTAAACTCCTGTCGTTAGGGACGAATC ATGACGGTACCTAACAAGAAAGCACCGGCTAACTACGTGCCAGCAGCCGCGG TAAAACGTAGGGTGCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCA GGCGGACCGGCAAGTTGGAAGTGAAAACCATGGGCTCAACCCATGAATTGCTT TCAAAACTGCTGGCCTTGAGTAGTGCAGAGGTAGGTGGAATTCCCGGTGTAGC GGTGGAATGCGTAGATATCGGGAGGAACACCAGTGGCGAAGGCGACCTACTG GGCACCAACTGACGCTGAGGCTCGAAAGCATGGGTAGCAAACAGGATTAGAT ACCCTGGTAGTCCATGCCGTAAACGATGATTACTAGGTGTTGGAGGATTGACC CCTTCAGTGCCGCAGTTAACACAATAAGTAATCCACCTGGGGAGTACGACCGC AAGGTTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCAGTGGAGTATG TGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTGACATCCGATGC ATAGTGCAGAGATGCATGAAGTCCTTCGGGACATCGAGACAGGTGGTGCATGG TTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAA CCCTTATTGCCAGTTACTACGTAAGAGGACTCTGGCGAGACTGCCGTTGACAA AACGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCTTTATGACCTGGGC TACACACGTACTACAATGGCGTTTAACAAAGAGAAGCAAGACCGCGAGGTGG AGCAAAACTCAGAAACAACGTCTCAGTTCAGATTGCAGGCTGCAACTCGCCTG CATGAAGTCGGAATTGCTAGTAATCGCGGATCAGCATGCCGCGGTGAATACGT TCCCGGGCCTTGTACACACCGCCCGTCACACCATGAGAGCCGGGGGGACCCGA AGTCGGTAGTCTA SEQ ID No: 18: 16S rRNA sequence of Eubacterium desmolans ATCC 43058 TTTTTAGAGAGTTTGATCCTGGCTCAGGATNAACGCTGGCGGCGTGC CTAACACATGCAAGTCGAACGGAGTTATTTTGGAAATCTCTTCGGAGATGGAA TTCATAACTTAGTGGCGGACGGGTGAGTAACGCGTGAGCAATCTGCCTTTAGG TGGGGGATAACAGTCGGAAACGGCTGCTAATACCGCATAATACGTTTTGGGGG CATCCTTGAAACGTCAAAGATTTATTGCCTTTAGATGAGCTCGCGTCTGATTAG CTGGTTGGCGGGGNAACGGCCCACCAAGGCGACGATCAGTAGCCGGACTGAG AGGTTGAACGGCCACATTGGGACTGAGACACGGCCCAGACTCCTACGGGAGG CAGCAGTGGGGAATATTGCGCAATGGGGGAAACCCNGACGCAGCAACGCCGC GTGATTGAAGAAGGCCTTCGGGTTGTAAAGATCTTTAATCAGGGACGAATTTT GACGGTACCTGAAGAATAAGCTCCGGCTAACTACGTGCCAGCAGCCGCGGTAA TACGTAGGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGCGCGCAGNC GGGNCGGCAAGTTGGGAGTGAAATCNGGGGGCTTAACCCCCGAACTGCTTTCA AAACTGCTGGTCTTGAGTGATGGAGAGGCAGGCGGAATTCCGTGTGTAGCGGT GAAATGCGTAGATATACGGAGGAACACCAGTGGCGAAGGCGGCCTGCTGGAC ATTAACTGACGCTGAGGCGCGAAAGCGTGGGGAGCAAACAGGATTAGATACC CTGGTAGTCCACGCCGTAAACGATGGATACTAGGTGTGGGAGGTATTGACCCC TTCCGTGCCGCAGTTAACACAATAAGTATCCCACCTGGGGAGTACGGCCGCAA GGTTGAAACTCAAAGGAATTGACGNNNGCCCGCACAAGCAGTGGAGTATGTG GTTTAATTCGAANNAACGCGAAGAACCTTACCAGGNCTTGACATCCCGGTGAC CGTCCTAGAGATAGGACTTNCCTTCGGGNCAACGGTGACAGNTGGTGCATGGT TGTCGTCAGCTCGTGTCGTGAGATGTTGGNTTAAGTCCCGCAACGAGCGCAAC CCTTACGGTTAGTTGATACGCAAGATCACTCTAGCCGGACTGCCGTTGACAAA ACGGAGGAAGGTGGGGACGACGTNNAATCATCATGCCCCNTATGACCTGGGCT ACACACGTACTACAATGGCAGTCATACAGAGGGAAGCAAAATCGCGAGGTGG AGCAAATCCCTAAAAGCTGTCCCAGTTCAGATTGCAGGCTGCAACCCGCCTGC

ATGAAGTCGGAATTGCTAGTAATCGCGGATCAGCATGCCGCGGTGAATACGTT CCCGGGNNTTGTACACACCGCCCGTCACACCATGAGAGCCGTCAATACCCGAA GTCCGTAGCCTAACCGCAAGGGGGGCGCGGCCGAAGGTAGGGGTGGTAAT

Sequence CWU 1

1

1811474DNAFaecalibacterium prausnitzii 1agagtttgat cctggctcag gacgaacgct ggcggcgcgc ctaacacatg caagtcgaac 60gagtgagaga gagcttgctt tctcgagcga gtggcgaacg ggtgagtaac gcgtgaggaa 120cctgcctcaa agagggggac aacagttgga aacgactgct aataccgcat aagcccacgg 180ctcggcatcg agcagaggga aaaggagtga tccgctttga gatggcctcg cgtccgatta 240gctagttggt gaggtaatgg cccaccaagg cgacgatcgg tagccggact gagaggttga 300acggccacat tgggactgag acacggccca gactcctacg ggaggcagca gtggggaata 360ttgcacaatg ggggaaaccc tgatgcagcg acgccgcgtg gaggaagaag gtcttcggat 420tgtaaactcc tgttgttggg gaagataatg acggtaccca acaaggaagt gacggctaac 480tacgtgccag cagccgcggt aaaacgtagg tcacaagcgt tgtccggaat tactgggtgt 540aaagggagcg caggcgggga gacaagttgg aagtgaaatc tatgggctca acccataaac 600tgctttcaaa actgtttttc ttgagtagtg cagaggtagg cggaattccc ggtgtagcgg 660tggaatgcgt agatatcggg aggaacacca gtggcgaagg cggcctactg ggcaccaact 720gacgctgagg ctcgaaagtg tgggtagcaa acaggattag ataccctggt agtccacacc 780gtaaacgatg attactaggt gttggaggat tgaccccttc agtgccgcag ttaacacaat 840aagtaatcca cctggggagt acgaccgcaa ggttgaaact caaaggaatt gacgggggcc 900cgcacaagca gtggagtatg tggtttaatt cgacgcaacg cgaagaacct taccaagtct 960tgacatccct tgacagacat agaaatatgt attctcttcg gagcaaggag acaggtggtg 1020catggttgtc gtcagctcgt gtcgtgagat gttgggttaa gtcccgcaac gagcgcaacc 1080cttatggtca gttactacgc aagaggactc tggccagact gccgttgaca aaacggagga 1140aggtggggat gacgtcaaat catcatgccc tttatgactt gggctacaca cgtactacaa 1200tggcgttaaa caaagagaag caagaccgcg aggtggagca aaactcagaa acaacgtccc 1260agttcggact gcaggctgca actcgcctgc acgaagtcgg aattgctagt aatcgtggat 1320cagcatgcca cggtgaatac gttcccgggc cttgtacaca ccgcccgtca caccatgaga 1380gccgggggga cccgaagtcg gtagtctaac cgcaaggagg acgccgccga aggtaaaact 1440ggtgattggg gtgaagtcgt aacaaggtag ccgt 147421474DNAFaecalibacterium prausnitzii 2agagtttgat cctggctcag gacgaacgct ggcggcgcgc ctaacacatg caagtcgaac 60gagcgagaga gagcttgctt tctcgagcga gtggcgaacg ggtgagtaac gcgtgaggaa 120cctgcctcaa agagggggac aacagttgga aacgactgct aataccgcat aagcccacgg 180gtcggcatcg accagaggga aaaggagcaa tccgctttga gatggcctcg cgtccgatta 240gctagttggt gaggtaatgg cccaccaagg caacgatcgg tagccggact gagaggttga 300acggccacat tgggactgag acgcggccca gactcctacg ggaggcagca gtggggaata 360ttgcacaatg ggggaaaccc tgatgcagcg acgccgcgtg gaggaagaag gtcttcggat 420tgtaaactcc tgttgttggg gaagataatg acggtaccca acaaggaagt gacggctaac 480tacgtgccag cagccgcggt aaaacgtagg tcacaagcgt tgtccggaat tactgggtgt 540aaagggagcg caggcgggaa gacaagttgg aagtgaaatc tatgggctca acccataaac 600tgctttcaaa actgtttttc ttgagtagtg cagaggtagg cggaattccc ggtgtagcgg 660tggaatgcgt agatatcggg aggaacacca gtggcgaagg cggcctactg ggcaccaact 720gacgctgagg ctcgaaagtg tgggtagcaa acaggattag ataccctggt agtccacacc 780gtaaacgatg gttactaggt gttggaggat tgaccccttc agtgccgcag ttaacacaat 840aagtaatcca cctggggagt acgaccgcaa ggttgaaact caaaggaatt gacgggggcc 900cgcacaagca gtggagtatg tggtttaatt cgacgcaacg cgaagaacct taccaagtct 960tgacatccct tgacagacat agaaatatgt aatctcttcg gagcaaggag acaggtggtg 1020catggttgtc gtcagctcgt gtcgtgagat gttgggttaa gtcccgcaac gagcgcaacc 1080cttatggtca gttactacgc aagaggactc tggccagact gccgttgaca aaacggagga 1140aggtggggat gacgtcaaat catcatgccc tttatgactt gggctacaca cgtactacaa 1200tggcgttaaa caaagagaag caagaccgcg aggtggagca aaactcagaa acaacgtccc 1260agttcggact gcaggctgca actcgcctgc acgaagtcgg aattgctagt aatcgtggat 1320cagcatgcca cggtgaatac gttcccgggc cttgtacaca ccgcccgtca caccatgaga 1380gccgggggga cccgaagtcg gtagtctaac cgcaaggagg acgccgccga aggtaaaact 1440ggtgattggg gtgaagtcgt aacaaggtag ccgt 147431408DNAFaecalibacterium prausnitzii 3gcgagagaga gcttgctttc tcgagcgagt ggcgaacggg tgagtaacgc gtgaggaacc 60tgcctcaaag agggggacaa cagttggaaa cgactgctaa taccgcataa gcccacggcc 120cggcatcggg cagagggaaa aggagcaatc cgctttgaga tggcctcgcg tccgattagc 180tagttggtga ggtaacggcc caccaaggcg acgatcggta gccggactga gaggttgaac 240ggccacattg ggactgagac acggcccaga ctcctacggg aggcagcagt ggggaatatt 300gcacaatggg ggaaaccctg atgcagcgac gccgcgtgga ggaagaaggt cttcggattg 360taaactcctg ttgttgagga agataatgac ggtactcaac aaggaagtga cggctaacta 420cgtgccagca gccgcggtaa aacgtaggtc acaagcgttg tccggaatta ctgggtgtaa 480agggagcgca ggcgggaaga caagttggaa gtgaaatcca tgggctcaac ccatgaactg 540ctttcaaaac tgtttttctt gagtagtgca gaggtaggcg gaattcccgg tgtagcggtg 600gaatgcgtag atatcgggag gaacaccagt ggcgaaggcg gcctactggg caccaactga 660cgctgaggct cgaaagtgtg ggtagcaaac aggattagat accctggtag tccacaccgt 720aaacgatgat tactaggtgt tggaggattg accccttcag tgccgcagtt aacacaataa 780gtaatccacc tggggagtac gaccgcaagg ttgaaactca aaggaattga cgggggcccg 840cacaagcagt ggagtatgtg gtttaattcg acgcaacgcg aagaacctta ccaagtcttg 900acatcctgcg acggttctgg aaacagaact ttccttcggg acgcagagac aggtggtgca 960tggttgtcgt cagctcgtgt cgtgagatgt tgggttaagt cccgcaacga gcgcaaccct 1020tatggtcagt tactacgcaa gaggactctg gccagactgc cgttgacaaa acggaggaag 1080gtggggatga cgtcaaatca tcatgccctt tatgacttgg gctacacacg tactacaatg 1140gcgttaaaca aagagaagca agaccgcgag gtggagcaaa actcagaaac aacgtcccag 1200ttcggactgc aggctgcaac tcgcctgcac gaagtcggaa ttgctagtaa tcgtggatca 1260gcatgccacg gtgaatacgt tcccgggcct tgtacacacc gcccgtcaca ccatgagagc 1320cggggggacc cgaagtcggt agtctaaccg caaggaggac gccgccgaag gtaaaactgg 1380tgattggggt gaagtcgtaa caaggtag 140841405DNAFaecalibacterium prausnitzii 4agcgagagag agcttgcttt ctcgagcgag tggcgaacgg gtgagtaacg cgtgaggaac 60ctgcctcaaa gagggggaca acagttggaa acgactgcta ataccgcata agcccacggt 120gccgcatggc acagagggaa aaggagcaat ccgctttgag atggcctcgc gtccgattag 180ctagttggtg aggtaacggc ccaccaaggc gacgatcggt agccggactg agaggttgaa 240cggccacatt gggactgaga cacggcccag actcctacgg gaggcagcag tggggaatat 300tgcacaatgg gggaaaccct gatgcagcga cgccgcgtgg aggaagaagg tcttcggatt 360gtaaactcct gttgttgggg aagataatga cggtacccaa caaggaagtg acggctaact 420acgtgccagc agccgcggta aaacgtaggt cacaagcgtt gtccggaatt actgggtgta 480aagggagcgc aggcgggaag acaagttgga agtgaaatct atgggctcaa cccataaact 540gctttcaaaa ctgtttttct tgagtagtgc agaggtaggc ggaattcccg gtgtagcggt 600ggaatgcgta gatatcggga ggaacaccag tggcgaaggc ggcctactgg gcaccaactg 660acgctgaggc tcgaaagtgt gggtagcaaa caggattaga taccctggta gtccacaccg 720taaacgatga ttactaggtg ttggaggatt gaccccttca gtgccgcagt taacacaata 780agtaatccac ctggggagta cgaccgcaag gttgaaactc aaaggaattg acgggggccc 840gcacaagcag tggagtatgt ggtttaattc gacgcaacgc gaagaacctt accaagtctt 900gacatccctt gacaggcata gaaatatgtt ttctcttcgg agcaaggaga caggtggtgc 960atggttgtcg tcagctcgtg tcgtgagatg ttgggttaag tcccgcaacg agcgcaaccc 1020ttatggtcag ttactacgca agaggactct ggccagactg ccgttgacaa aacggaggaa 1080ggtggggatg acgtcaaatc atcatgccct ttatgacttg ggctacacac gtactacaat 1140ggcgttaaac aaagagaagc aagaccgcga ggtggagcaa aactcagaaa caacgtccca 1200gttcggactg caggctgcaa ctcgcctgca cgaagtcgga attgctagta atcgtggatc 1260agcatgccac ggtgaatacg ttcccgggcc ttgtacacac cgcccgtcac accatgagag 1320ccggggggac ccgaagtcgg tagtctaacc gcaaggagga cgccgccgaa ggtaaaactg 1380gtgattgggg tgaagtcgta acaag 140551385DNAFaecalibacterium prausnitzii 5cgagtggcga acgggtgagt aacgcgtgag gaacctgcct caaagagggg gacaacagtt 60ggaaacgact gctaataccg cataagccca caggtcggca tcgaccagag ggaaaaggag 120caatccgctt tgagatggcc tcgcgtccga ttagctagtt ggtgaggtaa tggcccacca 180aggcaacgat cggtagccgg actgagaggt tgaacggcca cattgggact gagacacggc 240ccagactcct acgggaggca gcagtgggga atattgcaca atgggggaaa ccctgatgca 300gcgacgccgc gtggaggaag aaggtcttcg gattgtaaac tcctgttgtt gaggaagata 360atgacggtac tcaacaagga agtgacggct aactacgtgc cagcagccgc ggtaaaacgt 420aggtcacaag cgttgtccgg aattactggg tgtaaaggga gcgcaggcgg gaagacaagt 480tggaagtgaa atctatgggc tcaacccata aactgctttc aaaactgttt ttcttgagta 540gtgcagaggt aggcggaatt cccggtgtag cggtggaatg cgtagatatc gggaggaaca 600ccagtggcga aggcggccta ctgggcacca actgacgctg aggctcgaaa gtgtgggtag 660caaacaggat tagataccct ggtagtccac accgtaaacg atgattacta ggtgttggag 720gattgacccc ttcagtgccg cagttaacac aataagtaat ccacctgggg agtacgaccg 780caaggttgaa actcaaagga attgacgggg gcccgcacaa gcagtggagt atgtggttta 840attcgacgca acgcgaagaa ccttaccaag tcttgacatc ctgtgacgat gctggaaaca 900tgtttttcct tcggaacgca gagacaggtg gtgcatggtt gtcgtcagct cgtgtcgtga 960gatgttgggt taagtcccgc aacgagcgca acccttactg tcagttacta cgcaagagga 1020ctctggcagg actgccgttg acaaaacgga ggaaggtggg gatgacgtca aatcatcatg 1080ccctttatga cttgggctac acacgtacta caatggcgtt aaacaaagag aagcaagacc 1140gcgaggtgga gcaaaactca gaaacaacgt cccagttcgg actgcaggct gcaactcgcc 1200tgcacgaagt cggaattgct agtaatcgtg gatcagcatg ccacggtgaa tacgttcccg 1260ggccttgtac acaccgcccg tcacaccatg agagccgggg ggacccgaag tcggtagtct 1320aaccgcaagg aggacgccgc cgaaggtaaa actggtgatt ggggtgaagt cgtaacaagg 1380tagcc 138561405DNAFaecalibacterium prausnitzii 6cgagcgagag agagcttgct ttctcaatcg agtggcgaac gggtgagtaa cgcgtgagga 60acctgcctca aagaggggga caacagttgg aaacgactgc taataccgca taagcccaca 120ggtcggcatc gaccagaggg aaaaggagca atccgctttg agatggcctc gcgtccgatt 180agctagttgg tgaggtaatg gcccaccaag gcaacgatcg gtagccggac tgagaggttg 240aacggccaca ttgggactga gacacggccc agactcctac gggaggcagc agtggggaat 300attgcacaat gggggaaacc ctgatgcagc gacgccgcgt ggaggaagaa ggtcttcgga 360ttgtaaactc ctgttgttga ggaagataat gacggtactc aacaaggaag tgacggctaa 420ctacgtgcca gcagccgcgg taaaacgtag gtcacaagcg ttgtccggaa ttactgggtg 480taaagggagc gcaggcggga agacaagttg gaagtgaaat ctatgggctc aacccataaa 540ctgctttcaa aactgttttt cttgagtagt gcagaggtag gcggaattcc cggtgtagcg 600gtggaatgcg tagatatcgg gaggaacacc agtggcgaag gcggcctact gggcaccaac 660tgacgctgag gctcgaaagt gtgggtagca aacaggatta gataccctgg tagtccacac 720cgtaaacgat gattactagg tgttggagga ttgacccctt cagtgccgca gttaacacaa 780taagtaatcc acctggggag tacgaccgca aggttgaaac tcaaaggaat tgacgggggc 840ccgcacaagc agtggagtat gtggtttaat tcgacgcaac gcgaagaacc ttaccaagtc 900ttgacatcct gtgacgaacc tggaaatatg tttttccttc ggaacgcaga gacaggtggt 960gcatggttgt cgtcagctcg tgtcgtgaga tgttgggtta agtcccgcaa cgagcgcaac 1020ccttactgtc agttactacg caagaggact ctggcaggac tgccgttgac aaaacggagg 1080aaggtgggga tgacgtcaaa tcatcatgcc ctttatgact tgggctacac acgtactaca 1140atggcgttaa acaaagagaa gcaagaccgc gaggtggagc aaaactcaga aacaacgtcc 1200cagttcggac tgcaggctgc aactcgcctg cacgaagtcg gaattgctag taatcgtgga 1260tcagcatgcc acggtgaata cgttcccggg ccttgtacac accgcccgtc acaccatgag 1320agccgggggg acccgaagtc ggtagtctaa ccgcaaggag gacgccgccg aaggtaaaac 1380tggtgattgg ggtgaagtcg tacag 140571474DNAFaecalibacterium prausnitzii 7agagtttgat cctggctcag gacgaacgct ggcggcgcgc ctaacacatg caagtcgaac 60gagcgagaga gagcttgctt tctcgagcga gtggcgaacg ggtgagtaac gcgtggggaa 120cctgcctcaa agagggggac aacagttgga aacgactgct aataccgcat aagcccacga 180cctggcatcg ggttgaggga aaaggagcaa tccgctttga gatggcctcg cgtccgatta 240gctagttggt gaggtaatgg cccaccaagg caacgatcgg tagccggact gagaggttga 300acggccacat tgggactgag acacggccca gactcctacg ggaggcagca gtggggaata 360ttgcacaatg ggggaaaccc tgatgcagcg acgccgcgtg gaggaagaag gtcttcggat 420tgtaaactcc tgttgttgag gaagataatg acggtactca acaaggaagt gacggctaac 480tacgtgccag cagccgcggt aaaacgtagg tcacaagcgt tgtccggaat tactgggtgt 540aaagggagcg caggcgggaa gacaagttgg aagtgaaatc catgggctca acccatgaac 600tgctttcaaa actgtttttc ttgagtagtg cagaggtagg cggaattccc ggtgtagcgg 660tggaatgcgt agatatcggg aggaacacca gtggcgaagg cggcctactg ggcaccaact 720gacgctgagg ctcgaaagtg tgggtagcaa acaggattag ataccctggt agtccacacc 780gtaaacgatg attactaggt gttggaggat tgaccccttc agtgccgcag ttaacacaat 840aagtaatcca cctggggagt acgaccgcaa ggttgaaact caaaggaatt gacgggggcc 900cgcacaagca gtggagtatg tggtttaatt cgacgcaacg cgaagaacct taccaagtct 960tgacatcctg cgacggtgct ggaaacagtg ctttccttcg ggacgcagag acaggtggtg 1020catggttgtc gtcagctcgt gtcgtgagat gttgggttaa gtcccgcaac gagcgcaacc 1080cttatggtca gttactacgc aagaggactc tggccagact gccgttgaca aaacggagga 1140aggtggggat gacgtcaaat catcatgccc tttatgactt gggctacaca cgtactacaa 1200tggcgttaaa caaagagaag caagaccgcg aggtggagca aaactcagaa acaacgtccc 1260agttcggact gcaggctgca actcgcctgc acgaagtcgg aattgctagt aatcgtggat 1320cagcatgcca cggtgaatac gttcccgggc cttgtacaca ccgcccgtca caccatgaga 1380gccgggggga cccgaagtcg gtagtctaac cgcaaggagg acgccgccga aggtaaaact 1440ggtgattggg gtgaagtcgt aacaaggtag ccgt 147481533DNAFaecalibacterium prausnitzii 8gcatgctcga gcggccgcca gtgtgatgga tatctgcaga attcgccctt agagtttgat 60cctggctcag gacgaacgct ggcggcgcgc ctaacacatg caagtcgaac gagcgagaga 120gagcttgctt tctcgagcga gtggcgaacg ggtgagtaac gcgtgaggaa cctgcctcaa 180agagggggac aacagttgga aacgactgct aataccgcat aagcccacga cccggcatcg 240ggtagaggga aaaggagcaa tccgctttga gatggcctcg cgtccgatta gctagttggt 300gaggtaacgg cccaccaagg cgacgatcgg tagccggact gagaggttga acggccacat 360tgggactgag acacggccca gactcctacg ggaggcagca gtggggaata ttgcacaatg 420ggggaaaccc tgatgcagcg acgccgcgtg gaggaagaag gtcttcggat tgtaaactcc 480tgttgttgag gaagataatg acggtactca acaaggaagt gacggctaac tacgtgccag 540cagccgcggt aaaacgtagg tcacaagcgt tgtccggaat tactgggtgt aaagggagcg 600caggcgggaa gacaagttgg aagtgaaatc catgggctca acccatgaac tgctttcaaa 660actgtttttc ttgagtagtg cagaggtagg cggaattccc ggtgtagcgg tggaatgcgt 720agatatcggg aggaacacca gtggcgaagg cggcctactg ggcaccaact gacgctgagg 780ctcgaaagtg tgggtagcaa acaggattag ataccctggt agtccacacc gtaaacgatg 840attactaggt gttggaggat tgaccccttc agtgccgcag ttaacacaat aagtaatcca 900cctggggagt acgaccgcaa ggttgaaact caaaggaatt gacgggggcc cgcacaagca 960gtggagtatg tggtttaatt cgacgcaacg cgaagaacct taccaagtct tgacatcctg 1020cgacggttct ggaaacagaa ctttccttcg ggacgcagag acaggtggtg catggttgtc 1080gtcagctcgt gtcgtgagat gttgggttaa gtcccgcaac gagcgcaacc cttatggtca 1140gttactacgc aagaggactc tggccagact gccgttgaca aaacggagga aggtggggat 1200gacgtcaaat catcatgccc tttatgactt gggctacaca cgtactacaa tggcgttaaa 1260caaagagaag caagaccgcg aggtggagca aaactcagaa acaacgtccc agttcggact 1320gcaggctgca actcgcctgc acgaagtcgg aattgctagt aatcgtggat cagcatgcca 1380cggtgaatac gttcccgggc cttgtacaca ccgcccgtca caccatgaga gccgggggga 1440cccgaagtcg gtagtctaac cgcaaggagg acgccgccga aggtaaaact ggtgattggg 1500gtgaagtcgt aacaaggtag ccgtaagggc gaa 153391286DNAFaecalibacterium prausnitzii 9gcatcgggca gagggaaaag gagcaatccg ctttgagatg gcctcgcgtc cgattagcta 60gttggtgagg taatggccca ccaaggcgac gatcggtagc cggactgaga ggttgaacgg 120ccacattggg actgagacac ggcccagact cctacgggag gcagcagtgg ggaatattgc 180acaatggggg aaaccctgat gcagcgacgc cgcgtggagg aagaaggtct tcggattgta 240aactcctgtt gttgaggaag ataatgacgg tactcaacaa ggaagtgacg gctaactacg 300tgccagcagc cgcggtaaaa cgtaggtcac aagcgttgtc cggaattact gggtgtaaag 360ggagcgcagg cgggaagaca agttggaagt gaaatccatg ggctcaaccc atgaactgct 420ttcaaaactg tttttcttga gtagtgcaga ggtaggcgga attcccggtg tagcggtgga 480atgcgtagat atcgggagga acaccagtgg cgaaggcggc ctactgggca ccaactgacg 540ctgaggctcg aaagtgtggg tagcaaacag gattagatac cctggtagtc cacactgtaa 600acgatgatta ctaggtgttg gaggattgac cccttcagtg ccgcagttaa cacaataagt 660aatccacctg gggagtacga ccgcaaggtt gaaactcaaa ggaattgacg ggggcccgca 720caagcagtgg agtatgtggt ttaattcgac gcaacgcgaa gaaccttacc aagtcttgac 780atcctgcgac gcacatagaa atatgtgttt ccttcgggac gcagagacag gtggtgcatg 840gttgtcgtca gctcgtgtcg tgagatgttg ggttaagtcc cgcaacgagc gcaaccctta 900tggtcagtta ctacgcaaga ggactctggc cagactgccg ttgacaaaac ggaggaaggt 960ggggatgacg tcaaatcatc atgcccttta tgacttgggc tacacacgta ctacaatggc 1020gttaaacaaa gagaagcaag accgcgaggt ggagcaaaac tcagaaacaa cgtcccagtt 1080cggactgcag gctgcaactc gcctgcacga agtcggaatt gctagtaatc gcagatcagc 1140atgctgcggt gaatacgttc ccgggccttg tacacaccgc ccgtcacacc atgagagccg 1200gggggacccg aagtcggtag tctaaccgca aggaggacgc cgccgaaggt aaaactggtg 1260attggggtga agtcgtaaca aggtag 1286101407DNAFaecalibacterium prausnitzii 10cgagcgagag agagcttgct ttctcgagcg agtggcgaac gggtgagtaa cgcgtgagga 60acctgcctca aagaggggga caacagttgg aaacgactgc taataccgca taagcccacg 120ggtcggcatc gaccagaggg aaaaggagca atccgctttg agatggcctc gcgtccgatt 180agctagttgg tgaggtaacg gcccaccaag gcaacgatcg gtagccggac tgagaggttg 240aacggccaca ttgggactga gacacggccc agactcctac gggaggcagc agtggggaat 300attgcacaat gggggaaacc ctgatgcagc gacgccgcgt ggaggaagaa ggtcttcgga 360ttgtaaactc ctgttgttgg ggaagataat gacggtaccc aacaaggaag tgacggctaa 420ctacgtgcca gcagccgcgg taaaacgtag gtcacaagcg ttgtccggaa ttactgggtg 480taaagggagc gcaggcggga agacaagttg gaagtgaaat ctatgggctc aacccataaa 540ctgctttcaa aactgttttt cttgagtagt gcagaggtag gcggaattcc cggtgtagcg 600gtggaatgcg tagatatcgg gaggaacacc agtggcgaag gcggcctact gggcaccaac 660tgacgctgag gctcgaaagt gtgggtagca aacaggatta gataccctgg tagtccacac 720cgtaaacgat gattactagg tgttggagga ttgacccctt cagtgccgca gttaacacaa 780taagtaatcc acctggggag tacgaccgca aggttgaaac tcaaaggaat tgacgggggc 840ccgcacaagc agtggagtat gtggtttaat tcgacgcaac gcgaagaacc ttaccaagtc 900ttgacatccc ttgacagaca tagaaatatg taatctcttc ggagcaagga gacaggtggt 960gcatggttgt cgtcagctcg tgtcgtgaga tgttgggtta agtcccgcaa cgagcgcaac 1020ccttatggtc agttactacg caagaggact ctggccagac tgccgttgac aaaacggagg 1080aaggtgggga tgacgtcaaa tcatcatgcc ctttatgact tgggctacac acgtactaca 1140atggcgttaa acaaagagaa gcaagaccgc gaggtggagc aaaactcaga aacaacgtcc 1200cagttcggac tgcaggctgc aactcgcctg cacgaagtcg gaattgctag taatcgtgga 1260tcagcatgcc acggtgaata cgttcccggg ccttgtacac accgcccgtc acaccatgag 1320agccgggggg acccgaagtc ggtagtctaa ccgcaaggag gacgccgccg aaggtaaaac 1380tggtgattgg ggtgaagtcg taacaag

1407111382DNAFaecalibacterium prausnitzii 11gagcgagtgg cgaacgggtg agtaacgcgt gaggaacctg cctcaaagag ggggacaaca 60gttggaaacg actgctaata ccgcataagc ccacgacccg gcatcgggta gagggaaaag 120gagcaatccg ctttgagatg gcctcgcgtc cgattagcta gttggtgagg taatggccca 180ccaaggcgac gatcggtagc cggactgaga ggttgaacgg ccacattggg actgagacac 240ggcccagact cctacgggag gcagcagtgg ggaatattgc acaatggggg aaaccctgat 300gcagcgacgc cgcgtggagg aagaaggtct tcggattgta aactcctgtt gttgaggaag 360ataatgacgg tactcaacaa ggaagtgacg gctaactacg tgccagcagc cgcggtaaaa 420cgtaggtcac aagcgttgtc cggaattact gggtgtaaag ggagcgcagg cgggaagaca 480agttggaagt gaaatccatg ggctcaaccc atgaactgct ttcaaaactg tttttcttga 540gtagtgcaga ggtaggcgga attcccggtg tagcggtgga atgcgtagat atcgggagga 600acaccagtgg cgaaggcggc ctactgggca ccaactgacg ctgaggctcg aaagtgtggg 660tagcaaacag gattagatac cctggtagtc cacactgtaa acgatgatta ctaggtgttg 720gaggattgac cccttcagtg ccgcagttaa cacaataagt aatccacctg gggagtacga 780ccgcaaggtt gaaactcaaa ggaattgacg ggggcccgca caagcagtgg agtatgtggt 840ttaattcgac gcaacgcgaa gaaccttacc aagtcttgac atcctgcgac gcacatagaa 900atatgtgttt ccttcgggac gcagagacag gtggtgcatg gttgtcgtca gctcgtgtcg 960tgagatgttg ggttaagtcc cgcaacgagc gcaaccctta tggtcagtta ctacgcaaga 1020ggactctggc cagactgccg ttgacaaaac ggaggaaggt ggggatgacg tcaaatcatc 1080atgcccttta tgacttgggc tacacacgta ctacaatggc gttaaacaaa gagaagcaag 1140accgcgaggt ggagcaaaac tcagaaacaa cgtcccagtt cggactgcag gctgcaactc 1200gcctgcacga agtcggaatt gctagtaatc gcagatcagc atgctgcggt gaatacgttc 1260ccgggccttg tacacaccgc ccgtcacacc atgagagccg gggggacccg aagtcggtag 1320tctaaccgca aggaggacgc cgccgaaggt aaaactggtg attggggtga agtcgtaaca 1380ag 1382121395DNAFaecalibacterium prausnitzii 12agcttgcttt ctcgagcgag tggcgaacgg gtgagtaacg cgtgaggaac ctgcctcaaa 60gagggggaca acagttggaa acgactgcta ataccgcata agcccacggg tcggcatcga 120ccagagggaa aaggagcaat ccgctttgag atggcctcgc gtccgattag ctagttggtg 180aggtaacggc ccaccaaggc gacgatcggt agccggactg agaggttgaa cggccacatt 240gggactgaga cacggcccag actcctacgg gaggcagcag tggggaatat tgcacaatgg 300gggaaaccct gatgcagcga cgccgcgtgg aggaagaagg tcttcggatt gtaaactcct 360gttgttgggg aagataatga cggtacccaa caaggaagtg acggctaact acgtgccagc 420agccgcggta aaacgtaggt cacaagcgtt gtccggaatt actgggtgta aagggagcgc 480aggcgggaag acaagttgga agtgaaatct atgggctcaa cccataaact gctttcaaaa 540ctgtttttct tgagtagtgc agaggtaggc ggaattcccg gtgtagcggt ggaatgcgta 600gatatcggga ggaacaccag tggcgaaggc ggcctactgg gcaccaactg acgctgaggc 660tcgaaagtgt gggtagcaaa caggattaga taccctggta gtccacaccg taaacgatga 720ttactaggtg ttggaggatt gaccccttca gtgccgcagt taacacaata agtaatccac 780ctggggagta cgaccgcaag gttgaaactc aaaggaattg acgggggccc gcacaagcag 840tggagtatgt ggtttaattc gacgcaacgc gaagaacctt accaagtctt gacatccctt 900gacagacata gaaatatgta ttctcttcgg agcaaggaga caggtggtgc atggttgtcg 960tcagctcgtg tcgtgagatg ttgggttaag tcccgcaacg agcgcaaccc ttatggtcag 1020ttactacgca agaggactct ggccagactg ccgttgacaa aacggaggaa ggtggggatg 1080acgtcaaatc atcatgccct ttatgacttg ggctacacac gtactacaat ggcgttaaac 1140aaagagaagc aagaccgcga ggtggagcaa aactcagaaa caacgtccca gttcggactg 1200caggctgcaa ctcgcctgca cgaagtcgga attgctagta atcgtggatc agcatgccac 1260ggtgaatacg ttcccgggcc ttgtacacac cgcccgtcac accatgagag ccggggggac 1320ccgaagtcgg tagtctaacc gcaaggagga cgccgccgaa ggtaaaactg gtgattgggg 1380tgaagtcgta acaag 1395131390DNAFaecalibacterium prausnitzii 13atcgagtggc gaacgggtga gtaacgcgtg aggaacctgc ctcaaagagg gggacaacag 60ttggaaacga ctgctaatac cgcataagcc cacggctcgg catcgagcag agggaaaagg 120agcaatccgc tttgagatgg cctcgcgtcc gattagctag ttggtgaggt aatggcccac 180caaggcgacg atcggtagcc ggactgagag gttgaacggc cacattggga ctgagacacg 240gcccagactc ctacgggagg cagcagtggg gaatattgca caatggggga aaccctgatg 300cagcgacgcc gcgtggagga agaaggtctt cggattgtaa actcctgttg ttgaggaaga 360taatgacggt actcaacaag gaagtgacgg ctaactacgt gccagcagcc gcggtaaaac 420gtaggtcaca agcgttgtcc ggaattactg ggtgtaaagg gagcgcaggc gggcgatcaa 480gttggaagtg aaatccatgg gctcaaccca tgaactgctt tcaaaactga ttgtcttgag 540tagtgcagag gtaggcggaa ttcccggtgt agcggtggaa tgcgtagata tcgggaggaa 600caccagtggc gaaggcggcc tactgggcac caactgacgc tgaggctcga aagtgtgggt 660agcaaacagg attagatacc ctggtagtcc acaccgtaaa cgatgattac taggtgttgg 720aggattgacc ccttcagtgc cgcagttaac acaataagta atccacctgg ggagtacgac 780cgcaaggttg aaactcaaag gaattgacgg gggcccgcac aagcagtgga gtatgtggtt 840taattcgacg caacgcgaag aaccttacca agtcttgaca tcctgcgacg atgctagaaa 900tagtattttc cttcgggacg cagagacagg tggtgcatgg ttgtcgtcag ctcgtgtcgt 960gagatgttgg gttaagtccc gcaacgagcg caacccttat ggtcagttac tacgcaagag 1020gactctggcc agactgccgt tgacaaaacg gaggaaggtg gggatgacgt caaatcatca 1080tgccctttat gacttgggct acacacgtac tacaatggcg ttaaacaaag agaagcaaga 1140ccgcgaggtg gagcaaaact cagaaacaac gtcccagttc ggactgcagg ctgcaactcg 1200cctgcacgaa gtcggaattg ctagtaatcg cagatcagca tgctgcggtg aatacgttcc 1260cgggccttgt acacaccgcc cgtcacacca tgagagccgg ggggacccga agtcggtagt 1320ctaaccgcaa ggaggacgcc gccgaaggta aaactggtga ttggggtgaa gtcgtaacaa 1380ggtagccgtc 1390141400DNAFaecalibacterium prausnitzii 14gagagagctt gctttctcaa gcgagtggcg aacgggtgag taacgcgtga ggaacctgcc 60tcaaagaggg ggacaacagt tggaaacgac tgctaatacc gcataagccc acgacccggc 120atcgggtaga gggaaaagga gcaatccgct ttgagatggc ctcgcgtccg attagctagt 180tggtgaggta acggcccacc aaggcgacga tcggtagccg gactgagagg ttgaacggcc 240acattgggac tgagacacgg cccagactcc tacgggaggc agcagtgggg aatattgcac 300aatgggggaa accctgatgc agcgacgccg cgtggaggaa gaaggtcttc ggattgtaaa 360ctcctgttgt tgaggaagat aatgacggta ctcaacaagg aagtgacggc taactacgtg 420ccagcagccg cggtaaaacg taggtcacaa gcgttgtccg gaattactgg gtgtaaaggg 480agcgcaggcg ggaagacaag ttggaagtga aatccatggg ctcaacccat gaactgcttt 540caaaactgtt tttcttgagt agtgcagagg taggcggaat tcccggtgta gcggtggaat 600gcgtagatat cgggaggaac accagtggcg aaggcggcct actgggcacc aactgacgct 660gaggctcgaa agtgtgggta gcaaacagga ttagataccc tggtagtcca cactgtaaac 720gatgattact aggtgttgga ggattgaccc cttcagtgcc gcagttaaca caataagtaa 780tccacctggg gagtacgacc gcaaggttga aactcaaagg aattgacggg ggcccgcaca 840agcagtggag tatgtggttt aattcgacgc aacgcgaaga accttaccaa gtcttgacat 900cctgcgacgg tgctggaaac agtgctttcc ttcgggacgc agagacaggt ggtgcatggt 960tgtcgtcagc tcgtgtcgtg agatgttggg ttaagtcccg caacgagcgc aacccttatg 1020gtcagttact acgcaagagg actctggcca gactgccgtt gacaaaacgg aggaaggtgg 1080ggatgacgtc aaatcatcat gccctttatg acttgggcta cacacgtact acaatggcgt 1140taaacaaaga gaagcaagac cgcgaggtgg agcaaaactc agaaacaacg tcccagttcg 1200gactgcaggc tgcaactcgc ctgcacgaag tcggaattgc tagtaatcgc agatcagcat 1260gctgcggtga atacgttccc gggccttgta cacaccgccc gtcacaccat gagagccggg 1320gggacccgaa gtcggtagtc taaccgcaag gaggacgccg ccgaaggtaa aactggtgat 1380tggggtgaag tcgtaacaag 1400151388DNAFaecalibacterium prausnitzii 15caagcgagtg gcgaacgggt gagtaacgcg tgaggaacct gcctcaaaga gggggacaac 60agttggaaac gactgctaat accgcataag cccacgaccc ggcatcgggt agagggaaaa 120ggagcaatcc gctttgagat ggcctcgcgt ccgattagct agttggtgag gtaacggccc 180accaaggcga cgatcggtag ccggactgag aggttgaacg gccacattgg gactgagaca 240cggcccagac tcctacggga ggcagcagtg gggaatattg cacaatgggg gaaaccctga 300tgcagcgacg ccgcgtggag gaagaaggtc ttcggattgt aaactcctgt tgttgaggaa 360gataatgacg gtactcaaca aggaagtgac ggctaactac gtgccagcag ccgcggtaaa 420acgtaggtca caagcgttgt ccggaattac tgggtgtaaa gggagcgcag gcgggaagac 480aagttggaag tgaaatccat gggctcaacc catgaactgc tttcaaaact gtttttcttg 540agtagtgcag aggtaggcgg aattcccggt gtagcggtgg aatgcgtaga tatcgggagg 600aacaccagtg gcgaaggcgg cctactgggc accaactgac gctgaggctc gaaagtgtgg 660gtagcaaaca ggattagata ccctggtagt ccacactgta aacgatgatt actaggtgtt 720ggaggattga ccccttcagt gccgcagtta acacaataag taatccacct ggggagtacg 780accgcaaggt tgaaactcaa aggaattgac gggggcccgc acaagcagtg gagtatgtgg 840tttaattcga cgcaacgcga agaaccttac caagtcttga catcctgcga cgcacataga 900aatatgtgtt tccttcggga cgcagagaca ggtggtgcat ggttgtcgtc agctcgtgtc 960gtgagatgtt gggttaagtc ccgcaacgag cgcaaccctt atggtcagtt actacgcaag 1020aggactctgg ccagactgcc gttgacaaaa cggaggaagg tggggatgac gtcaaatcat 1080catgcccttt atgacttggg ctacacacgt actacaatgg cgttaaacaa agagaagcaa 1140gaccgcgagg tggagcaaaa ctcagaaaca acgtcccagt tcggactgca ggctgcaact 1200cgcctgcacg aagtcggaat tgctagtaat cgcagatcag catgctgcgg tgaatacgtt 1260cccgggcctt gtacacaccg cccgtcacac catgagagcc ggggggaccc gaagtcggta 1320gtctaaccgc aaggaggacg ccgccgaagg taaaactggt gattggggtg aagtcgtaac 1380aagggtag 1388161474DNAFaecalibacterium prausnitzii 16agagtttgat cctggctcag gacgaacgct ggcggcgcgc ctaacacatg caagtcgaac 60gagcgagaga gagcttgctt tctcgagcga gtggcgaacg ggtgagtaac gcgtgaggaa 120cctgcctcaa agagggggac aacagttgga aacgactgct aataccgcat aagcccacga 180cccggcatcg ggttgaggga aaaggagcaa tccgctttga gatggcctcg cgtccgatta 240gctagttggt gaggtaacgg cccaccaagg cgacgatcgg tagccggact gagaggttga 300acggccacat tgggactgag acacggccca gactcctacg ggaggcagca gtggggaata 360ttgcacaatg ggggaaaccc tgatgcagcg acgccgcgtg gaggaagaag gtcttcggat 420tgtaaactcc tgttgttggg gaagataatg acggtaccca acaaggaagt gacggctaac 480tacgtgccag cagccgcggt aaaacgtagg tcacaagcgt tgtccggaat tactgggtgt 540aaagggagcg caggcgggaa gacaagttgg aagtgaaatc catgggctta acccatgaac 600tgctttcaaa actgtttttc ttgagtagtg cagaggtagg cggaattccc ggtgtagcgg 660tggaatgcgt agatatcggg aggaacacca gtggcgaagg cggcctactg ggcaccaact 720gacgctgagg ctcgaaagtg tgggtagcaa acaggattag ataccctggt agtccacacc 780gtaaacgatg attactaggt gttggaggat tgaccccttc agtgccgcag ttaacacaat 840aagtaatcca cctggggagt acgaccgcaa ggttgaaact caaaggaatt gacgggggcc 900cgcacaagca gtggagtatg tggtttaatt cgacgcaacg cgaagaacct taccaagtct 960tgacatcctg tgacagacgt agaaatacgt tcttccttcg ggacacagag acaggtggtg 1020catggttgtc gtcagctcgt gtcgtgagat gttgggttaa gtcccgcaac gagcgcaacc 1080cttatggtca gttactacgc aagaggactc tggccagact gccgttgaca aaacggagga 1140aggtggggat gacgtcaaat catcatgccc tttatgactt gggctacaca cgtactacaa 1200tggcgttaaa caaagagaag caagaccgcg aggtggagca aaactcagaa acaacgtccc 1260agttcggact gcaggctgca actcgcctgc acgaagtcgg aattgctagt aatcgtggat 1320cagcatgcca cggtgaatac gttcccgggc cttgtacaca ccgcccgtca caccatgaga 1380gccgggggga cccgaagtcg gtagtctaac cgcaaggagg acgccgccga aggtaaaact 1440ggtgattggg gtgaagtcgt aacaaggtag ccgt 1474171323DNAFaecalibacterium prausnitzii 17ttagtggcga acgggtgagt aacgcgtgag taacctgccc tggagtgggg gacaacagtt 60ggaaacgact gctaataccg cataagccca cggcccggca tcgggctgcg ggaaaaggat 120ttattcgctt caggatggac tcgcgtccaa ttagctagtt ggtgaggtaa cggcccacca 180aggcgacgat tggtagccgg actgagaggt tgaacggcca cattgggact gagacacggc 240ccagactcct acgggaggca gcagtggggg atattgcaca atgggggaaa ccctgatgca 300gcgacgccgc gtggaggaag aaggttttcg gattgtaaac tcctgtcgtt agggacgaat 360catgacggta cctaacaaga aagcaccggc taactacgtg ccagcagccg cggtaaaacg 420tagggtgcaa gcgttgtccg gaattactgg gtgtaaaggg agcgcaggcg gaccggcaag 480ttggaagtga aaaccatggg ctcaacccat gaattgcttt caaaactgct ggccttgagt 540agtgcagagg taggtggaat tcccggtgta gcggtggaat gcgtagatat cgggaggaac 600accagtggcg aaggcgacct actgggcacc aactgacgct gaggctcgaa agcatgggta 660gcaaacagga ttagataccc tggtagtcca tgccgtaaac gatgattact aggtgttgga 720ggattgaccc cttcagtgcc gcagttaaca caataagtaa tccacctggg gagtacgacc 780gcaaggttga aactcaaagg aattgacggg ggcccgcaca agcagtggag tatgtggttt 840aattcgaagc aacgcgaaga accttaccag gtcttgacat ccgatgcata gtgcagagat 900gcatgaagtc cttcgggaca tcgagacagg tggtgcatgg ttgtcgtcag ctcgtgtcgt 960gagatgttgg gttaagtccc gcaacgagcg caacccttat tgccagttac tacgtaagag 1020gactctggcg agactgccgt tgacaaaacg gaggaaggtg gggatgacgt caaatcatca 1080tgccctttat gacctgggct acacacgtac tacaatggcg tttaacaaag agaagcaaga 1140ccgcgaggtg gagcaaaact cagaaacaac gtctcagttc agattgcagg ctgcaactcg 1200cctgcatgaa gtcggaattg ctagtaatcg cggatcagca tgccgcggtg aatacgttcc 1260cgggccttgt acacaccgcc cgtcacacca tgagagccgg ggggacccga agtcggtagt 1320cta 1323181469DNAEubacterium desmolansmisc_feature(31)..(31)n is a, c, g, t or umisc_feature(274)..(274)n is a, c, g, t or umisc_feature(400)..(400)n is a, c, g, t or umisc_feature(574)..(574)n is a, c, g, t or umisc_feature(579)..(579)n is a, c, g, t or umisc_feature(601)..(601)n is a, c, g, t or umisc_feature(916)..(918)n is a, c, g, t or umisc_feature(956)..(957)n is a, c, g, t or umisc_feature(979)..(979)n is a, c, g, t or umisc_feature(1016)..(1016)n is a, c, g, t or umisc_feature(1025)..(1025)n is a, c, g, t or umisc_feature(1038)..(1038)n is a, c, g, t or umisc_feature(1079)..(1079)n is a, c, g, t or umisc_feature(1179)..(1180)n is a, c, g, t or umisc_feature(1195)..(1195)n is a, c, g, t or umisc_feature(1373)..(1374)n is a, c, g, t or u 18tttttagaga gtttgatcct ggctcaggat naacgctggc ggcgtgccta acacatgcaa 60gtcgaacgga gttattttgg aaatctcttc ggagatggaa ttcataactt agtggcggac 120gggtgagtaa cgcgtgagca atctgccttt aggtggggga taacagtcgg aaacggctgc 180taataccgca taatacgttt tgggggcatc cttgaaacgt caaagattta ttgcctttag 240atgagctcgc gtctgattag ctggttggcg gggnaacggc ccaccaaggc gacgatcagt 300agccggactg agaggttgaa cggccacatt gggactgaga cacggcccag actcctacgg 360gaggcagcag tggggaatat tgcgcaatgg gggaaacccn gacgcagcaa cgccgcgtga 420ttgaagaagg ccttcgggtt gtaaagatct ttaatcaggg acgaattttg acggtacctg 480aagaataagc tccggctaac tacgtgccag cagccgcggt aatacgtagg gagcaagcgt 540tatccggatt tactgggtgt aaagggcgcg cagncgggnc ggcaagttgg gagtgaaatc 600ngggggctta acccccgaac tgctttcaaa actgctggtc ttgagtgatg gagaggcagg 660cggaattccg tgtgtagcgg tgaaatgcgt agatatacgg aggaacacca gtggcgaagg 720cggcctgctg gacattaact gacgctgagg cgcgaaagcg tggggagcaa acaggattag 780ataccctggt agtccacgcc gtaaacgatg gatactaggt gtgggaggta ttgacccctt 840ccgtgccgca gttaacacaa taagtatccc acctggggag tacggccgca aggttgaaac 900tcaaaggaat tgacgnnngc ccgcacaagc agtggagtat gtggtttaat tcgaannaac 960gcgaagaacc ttaccaggnc ttgacatccc ggtgaccgtc ctagagatag gacttncctt 1020cgggncaacg gtgacagntg gtgcatggtt gtcgtcagct cgtgtcgtga gatgttggnt 1080taagtcccgc aacgagcgca acccttacgg ttagttgata cgcaagatca ctctagccgg 1140actgccgttg acaaaacgga ggaaggtggg gacgacgtnn aatcatcatg ccccntatga 1200cctgggctac acacgtacta caatggcagt catacagagg gaagcaaaat cgcgaggtgg 1260agcaaatccc taaaagctgt cccagttcag attgcaggct gcaacccgcc tgcatgaagt 1320cggaattgct agtaatcgcg gatcagcatg ccgcggtgaa tacgttcccg ggnnttgtac 1380acaccgcccg tcacaccatg agagccgtca atacccgaag tccgtagcct aaccgcaagg 1440ggggcgcggc cgaaggtagg ggtggtaat 1469

Claims

1. A method of treating and/or preventing visceral abdominal pain in an individual, comprising administering to the individual a therapeutically effective amount of at least one Faecalibacterium prausnitzii bacterial strain belonging to phylogroup I, II and/or III.

2. The method according to claim 1, wherein the at least one Faecalibacterium prausnitzii bacterial strain belongs to phylogroup I and/or phylogroup III.

3. The method according to claim 1, wherein the at least one Faecalibacterium prausnitzii bacterial strain belongs to phylogroup I and is selected from the group consisting of bacterial strains deposited to the CNCM under accession numbers I-4542, I-4544 and I-4540.

4. The method according to claim 1, wherein the at least one Faecalibacterium prausnitzii bacterial strain belongs to phylogroup III and is selected from the group consisting of bacterial strains deposited to the CNCM under accession numbers I-4574, I-4543, I-4575, I-4573, I-4644 and I-4546.

5. The method according to claim 1, wherein the at least one Faecalibacterium prausnitzii bacterial strain is selected from the group consisting of bacterial strains deposited to the CNCM under the accession numbers I-4542, I-4544, I-4540, I-4574, I-4543, I-4575, I-4573, I-4644 and I-4546.

6. The method according to claim 1, wherein the visceral abdominal pain is a gastrointestinal pain.

7. The method according to claim 1, wherein the visceral abdominal pain is caused by a gastrointestinal disorder.

8. The method according to claim 7, wherein the gastrointestinal disorder is a gastrointestinal hypersensitivity.

9. The method according to claim 1, wherein the bacterial strain is comprised in a composition comprising a physiologically acceptable medium.

10. A composition comprising, in a physiologically acceptable medium, at least one bacterial strain of Faecalibacterium prausnitzii selected from the group consisting of bacterial strains deposited to the CNCM under the accession numbers I-4542, I-4544, I-4540, I-4574, I-4543, I-4575, I-4573, I-4644 and I-4546.

11. The composition according to claim 10, wherein the at least one bacterial strain of Faecalibacterium prausnitzii is the bacterial strain deposited to the CNCM under the accession number I-4573.

12. The composition according to claim 10, wherein the composition is for oral administration and is selected from the group consisting of a food product, a drink, a pharmaceutical, a nutraceutical, a food additive, a food supplement and a dairy product.

13. An isolated bacterial strain selected from the group consisting of bacterial strains deposited to the CNCM under the accession numbers I-4542, I-4544, I-4540, I-4574, I-4543, I-4575, I-4573, I-4644 and I-4546.

14. The isolated bacterial strain according to claim 13, being the bacterial strain deposited to the CNCM under the accession number I-4573.

15. The method of claim 5, wherein the at least one Faecalibacterium prausnitzii bacterial strain is a bacterial strain deposited to the CNCM under accession number I-4573.

16. The method of claim 6, wherein the gastrointestinal pain is a colonic pain.

17. The method according to claim 7, wherein the gastrointestinal disorder is a non-inflammatory gastrointestinal disorder.

18. The method according to claim 8, wherein the gastrointestinal hypersensitivity is a colonic hypersensitivity selected from Irritable Bowel Syndrome and alternating-type Irritable Bowel Syndrome.

19. The method according to claim 9, wherein the physiologically acceptable medium is a food supplement.

20. The composition of claim 12, wherein the composition is a food supplement.

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