U.S. patent application number 15/978290 was filed with the patent office on 2019-03-21 for aav-mediated subcellular targeting of heterologous rhodopsins in retinal ganglion cells. The applicant listed for this patent is WAYNE STATE UNIVERSITY. Invention is credited to Zhuo-Hua PAN.
Application Number | 20190083651 15/978290 |
Document ID | / |
Family ID | 44904064 |
Filed Date | 2019-03-21 |
United States Patent Application | 20190083651 |
Kind Code | A1 |
PAN; Zhuo-Hua | March 21, 2019 |
Microbial type rhodopsins, such as the light-gated cation-selective membrane channel, channelrhodopsin-2 (Chop2/ChR2) or the ion pump halorhodopsin (HaloR) are expressed in retinal ganglion cells upon transduction using recombinant AAV vectors. Selective targeting of these transgenes for expression in discrete subcellular regions or sites is achieved by including a sorting motif in the vector that can target either the central area or surround (off-center) area of these cells. Nucleic acid molecules comprising nucleotide sequences encoding such rhodopsins and sorting motifs and their use in methods of differential expression of the transgene are disclosed. These compositions and methods provide significant improvements for restoring visual perception and various aspects of vision, particular in patients with retinal disease.
Inventors: | PAN; Zhuo-Hua; (Troy, MI) | ||||||||||
Applicant: |
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Family ID: | 44904064 | ||||||||||
Appl. No.: | 15/978290 | ||||||||||
Filed: | May 14, 2018 |
Application Number | Filing Date | Patent Number | ||
---|---|---|---|---|
15236152 | Aug 12, 2016 | 9968689 | ||
15978290 | ||||
13696252 | Jun 12, 2013 | 9453241 | ||
PCT/US11/35266 | May 4, 2011 | |||
15236152 | ||||
61331125 | May 4, 2010 | |||
Current U.S. Class: | 1/1 |
Current CPC Class: | A61K 48/005 20130101; A61P 27/02 20180101; C07K 14/705 20130101; C12N 15/8616 20130101; C07K 14/4702 20130101; A61K 38/00 20130101; C07K 2319/01 20130101; C12N 2750/14143 20130101 |
International Class: | A61K 48/00 20060101 A61K048/00; C12N 15/861 20060101 C12N015/861; C07K 14/47 20060101 C07K014/47; C07K 14/705 20060101 C07K014/705 |
Sequence CWU 1 SEQUENCE LISTING <160> NUMBER OF SEQ ID
NOS: 47 <210> SEQ ID NO 1 <211> LENGTH: 195 <212>
TYPE: DNA <213> ORGANISM: Artificial Sequence <220>
FEATURE: <223> OTHER INFORMATION: Kv2.1 Cytoplasmic
C-terminus sorting motif <400> SEQUENCE: 1 cagtctcagc
ccatcctgaa cactaaggag atggcccctc agagtaaacc ccctgaggaa 60
ctggaaatga gctccatgcc atctccagtg gctcctctgc cagctaggac cgagggcgtg
120 attgacatga gaagcatgtc tagtatcgat agcttcattt cctgcgccac
cgacttcccc 180 gaagctacaa ggttt 195 <210> SEQ ID NO 2
<211> LENGTH: 65 <212> TYPE: PRT <213> ORGANISM:
Artificial Sequence <220> FEATURE: <223> OTHER
INFORMATION: Kv2.1 Cytoplasmic C-terminus sorting motif <400>
SEQUENCE: 2 Gln Ser Gln Pro Ile Leu Asn Thr Lys Glu Met Ala Pro Gln
Ser Lys 1 5 10 15 Pro Pro Glu Glu Leu Glu Met Ser Ser Met Pro Ser
Pro Val Ala Pro 20 25 30 Leu Pro Ala Arg Thr Glu Gly Val Ile Asp
Met Arg Ser Met Ser Ser 35 40 45 Ile Asp Ser Phe Ile Ser Cys Ala
Thr Asp Phe Pro Glu Ala Thr Arg 50 55 60 Phe 65 <210> SEQ ID
NO 3 <211> LENGTH: 81 <212> TYPE: DNA <213>
ORGANISM: Artificial Sequence <220> FEATURE: <223>
OTHER INFORMATION: Nav1.6 Ankyrin binding domain sorting motif
<400> SEQUENCE: 3 accgtgaggg tgcccatcgc cgtgggcgag agcgacttcg
agaacctgaa caccgaggac 60 gtgagcagcg agagcgaccc c 81 <210> SEQ
ID NO 4 <211> LENGTH: 27 <212> TYPE: PRT <213>
ORGANISM: Artificial Sequence <220> FEATURE: <223>
OTHER INFORMATION: Nav1.6 Ankyrin binding domain sorting motif
<400> SEQUENCE: 4 Thr Val Arg Val Pro Ile Ala Val Gly Glu Ser
Asp Phe Glu Asn Leu 1 5 10 15 Asn Thr Glu Asp Val Ser Ser Glu Ser
Asp Pro 20 25 <210> SEQ ID NO 5 <211> LENGTH: 96
<212> TYPE: DNA <213> ORGANISM: Artificial Sequence
<220> FEATURE: <223> OTHER INFORMATION: NLG-1
Cytoplasmic C-terminal sorting motif <400> SEQUENCE: 5
gtggtgctga ggactgcctg cccccctgac tacaccctgg ctatgaggag aagcccagac
60 gatgtgcccc tgatgacccc caacaccatc acaatg 96 <210> SEQ ID NO
6 <211> LENGTH: 32 <212> TYPE: PRT <213>
ORGANISM: Artificial Sequence <220> FEATURE: <223>
OTHER INFORMATION: NLG-1 Cytoplasmic C-terminal sorting motif
<400> SEQUENCE: 6 Val Val Leu Arg Thr Ala Cys Pro Pro Asp Tyr
Thr Leu Ala Met Arg 1 5 10 15 Arg Ser Pro Asp Asp Val Pro Leu Met
Thr Pro Asn Thr Ile Thr Met 20 25 30 <210> SEQ ID NO 7
<211> LENGTH: 78 <212> TYPE: DNA <213> ORGANISM:
Artificial Sequence <220> FEATURE: <223> OTHER
INFORMATION: MLPH Myosin binding domain sorting motif <400>
SEQUENCE: 7 agggaccagc ctctgaacag caaaaagaaa aagaggctcc tgagcttcag
ggacgtggac 60 ttcgaggagg acagcgac 78 <210> SEQ ID NO 8
<211> LENGTH: 26 <212> TYPE: PRT <213> ORGANISM:
Artificial Sequence <220> FEATURE: <223> OTHER
INFORMATION: MLPH Myosin binding domain sorting motif <400>
SEQUENCE: 8 Arg Asp Gln Pro Leu Asn Ser Lys Lys Lys Lys Arg Leu Leu
Ser Phe 1 5 10 15 Arg Asp Val Asp Phe Glu Glu Asp Ser Asp 20 25
<210> SEQ ID NO 9 <211> LENGTH: 141 <212> TYPE:
DNA <213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: nAchR Tyrosine-Dileucine sorting
motif <400> SEQUENCE: 9 ggcgaggaca aggtgcggcc cgcctgtcag
cacaagcctc ggcggtgcag cctggccagc 60 gtggagctga gcgccggcgc
cggcccaccc accagcaacg gcaacctgct gtacatcggc 120 ttcagaggcc
tggagggcat g 141 <210> SEQ ID NO 10 <211> LENGTH: 47
<212> TYPE: PRT <213> ORGANISM: Artificial Sequence
<220> FEATURE: <223> OTHER INFORMATION: nAchR
Tyrosine-Dileucine sorting motif <400> SEQUENCE: 10 Gly Glu
Asp Lys Val Arg Pro Ala Cys Gln His Lys Pro Arg Arg Cys 1 5 10 15
Ala Leu Ala Ser Val Glu Leu Ser Ala Gly Ala Gly Pro Pro Thr Ser 20
25 30 Asn Gly Asn Leu Leu Tyr Ile Gly Phe Arg Gly Leu Glu Gly Met
35 40 45 <210> SEQ ID NO 11 <211> LENGTH: 48
<212> TYPE: DNA <213> ORGANISM: Artificial Sequence
<220> FEATURE: <223> OTHER INFORMATION: Kv4.2 Dileucine
sorting motif <400> SEQUENCE: 11 ttcgagcagc agcaccacca
cctgctgcac tgcctggaga agaccacc 48 <210> SEQ ID NO 12
<211> LENGTH: 16 <212> TYPE: PRT <213> ORGANISM:
Artificial Sequence <220> FEATURE: <223> OTHER
INFORMATION: Kv4.2 Dileucine sorting motif <400> SEQUENCE: 12
Phe Glu Gln Gln His His His Leu Leu His Cys Leu Glu Lys Thr Thr 1 5
10 15 <210> SEQ ID NO 13 <211> LENGTH: 192 <212>
TYPE: DNA <213> ORGANISM: Artificial Sequence <220>
FEATURE: <223> OTHER INFORMATION: TLCN Phenylalanine-based
sorting motif <400> SEQUENCE: 13 cagagcacag cctgcaaaaa
gggcgagtac aacgtgcagg aagctgagag ctctggcgaa 60 gccgtgtgtc
tgaacggcgc cggaggcggt gccggcggag ctgccggcgc tgagggtggc 120
cctgaggccg ctggaggtgc cgctgagagc cccgctgagg gcgaagtctt tgccatccag
180 ctgacatctg ct 192 <210> SEQ ID NO 14 <211> LENGTH:
64 <212> TYPE: PRT <213> ORGANISM: Artificial Sequence
<220> FEATURE: <223> OTHER INFORMATION: TLCN
Phenylalanine-based sorting motif <400> SEQUENCE: 14 Gln Ser
Thr Ala Cys Lys Lys Gly Glu Tyr Asn Val Gln Glu Ala Glu 1 5 10 15
Ser Ser Gly Glu Ala Val Cys Leu Asn Gly Ala Gly Gly Gly Ala Gly 20
25 30 Gly Ala Ala Gly Ala Glu Gly Gly Pro Glu Ala Ala Gly Gly Ala
Ala 35 40 45 Glu Ser Pro Ala Glu Gly Glu Val Phe Ala Ile Gln Leu
Thr Ser Ala 50 55 60 <210> SEQ ID NO 15 <211> LENGTH:
117 <212> TYPE: DNA <213> ORGANISM: Artificial Sequence
<220> FEATURE: <223> OTHER INFORMATION: AMPAR
Cytoplasmic C-terminal sorting motif <400> SEQUENCE: 15
gagttctgct acaagagcag gtccgaatct aagagaatga aaggcttttg tctgatcccc
60 cagcagagca tcaacgaggc cattcggacc agtacactgc ctcgcaatag cggagct
117 <210> SEQ ID NO 16 <211> LENGTH: 39 <212>
TYPE: PRT <213> ORGANISM: Artificial Sequence <220>
FEATURE: <223> OTHER INFORMATION: AMPAR Cytoplasmic
C-terminal sorting motif <400> SEQUENCE: 16 Glu Phe Cys Tyr
Lys Ser Arg Ser Glu Ser Lys Arg Met Lys Gly Phe 1 5 10 15 Cys Leu
Ile Pro Gln Gln Ser Ile Asn Glu Ala Ile Arg Thr Ser Thr 20 25 30
Leu Pro Arg Asn Ser Gly Ala 35 <210> SEQ ID NO 17 <211>
LENGTH: 141 <212> TYPE: DNA <213> ORGANISM: Artificial
Sequence <220> FEATURE: <223> OTHER INFORMATION: AAV2
5' ITR <400> SEQUENCE: 17 cctgcaggca gctgcgcgct cgctcgctca
ctgaggccgc ccgggcaaag cccgggcgtc 60 gggcgacctt tggtcgcccg
gcctcagtga gcgagcgagc gcgcagagag ggagtggcca 120 actccatcac
taggggttcc t 141 <210> SEQ ID NO 18 <211> LENGTH: 141
<212> TYPE: DNA <213> ORGANISM: Artificial Sequence
<220> FEATURE: <223> OTHER INFORMATION: AAV2 3' ITR
<400> SEQUENCE: 18 aggaacccct agtgatggag ttggccactc
cctctctgcg cgctcgctcg ctcactgagg 60 ccgggcgacc aaaggtcgcc
cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc 120 gagcgcgcag
ctgcctgcag g 141 <210> SEQ ID NO 19 <400> SEQUENCE: 19
000 <210> SEQ ID NO 20 <400> SEQUENCE: 20 000
<210> SEQ ID NO 21 <211> LENGTH: 945 <212> TYPE:
DNA <213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: ChR2 <400> SEQUENCE: 21
atggattatg gaggcgccct gagtgccgtt gggcgcgagc tgctatttgt aacgaaccca
60 gtagtcgtca atggctctgt acttgtgcct gaggaccagt gttactgcgc
gggctggatt 120 gagtcgcgtg gcacaaacgg tgcccaaacg gcgtcgaacg
tgctgcaatg gcttgctgct 180 ggcttctcca tcctactgct tatgttttac
gcctaccaaa catggaagtc aacctgcggc 240 tgggaggaga tctatgtgtg
cgctatcgag atggtcaagg tgattcttga gttcttcttc 300 gagtttaaga
acccgtccat gctgtatcta gccacaggcc accgcgtcca gtggttgcgt 360
tacgccgagt ggcttctcac ctgcccggtc attctcattc acctgtcaaa cctgacgggc
420 ttgtccaacg actacagcag gcgcactatg ggtctgcttg tgtctgatat
tggcacaatt 480 gtgtggggcg ccacttccgc tatggccacc ggatacgtca
aggtcatctt cttctgcctg 540 ggtctgtgtt atggtgctaa cacgttcttt
cacgctgcca aggcctacat cgagggttac 600 cataccgtgc cgaagggccg
gtgtcgccag gtggtgactg gcatggcttg gctcttcttc 660 gtatcatggg
gtatgttccc catcctgttc atcctcggcc ccgagggctt cggcgtcctg 720
agcgtgtacg gctccaccgt cggccacacc atcattgacc tgatgtcgaa gaactgctgg
780 ggtctgctcg gccactacct gcgcgtgctg atccacgagc atatcctcat
ccacggcgac 840 attcgcaaga ccaccaaatt gaacattggt ggcactgaga
ttgaggtcga gacgctggtg 900 gaggacgagg ccgaggctgg cgcggtcaac
aagggcaccg gcaag 945 <210> SEQ ID NO 22 <400> SEQUENCE:
22 000 <210> SEQ ID NO 23 <211> LENGTH: 873 <212>
TYPE: DNA <213> ORGANISM: Artificial Sequence <220>
FEATURE: <223> OTHER INFORMATION: HaloR <400> SEQUENCE:
23 atgactgaga cattgccacc ggtaacggaa tcggctgttg cgctacaggc
ggaggtgacc 60 cagagggagc tgttcgagtt cgttctcaac gaccccctcc
tcgccagttc gctgtatatt 120 aatatcgcac tggcagggct gtcgatactg
cttttcgtgt tcatgacgcg cggactcgac 180 gacccacggg cgaaactcat
cgccgtttcg acgattttgg tgccggtggt ctctatcgcg 240 agctacaccg
gccttgcatc ggggctcacc atcagcgtcc tcgagatgcc agccggccac 300
ttcgccgagg ggtcctcggt gatgctcggc ggcgaagagg tagacggcgt cgtgacgatg
360 tggggccgct atctgacgtg ggccctttcg acaccgatga tactgctggc
gcttgggctg 420 cttgctggct ctaacgccac gaagctcttt accgccatca
ccttcgacat cgcgatgtgt 480 gtcaccggcc tcgcagccgc gctgacgacc
tcttcgcacc tgatgcggtg gttctggtac 540 gccatcagtt gtgcgtgttt
cctcgtcgtc ctctacatcc tgctcgtcga gtgggcacag 600 gacgccaagg
ctgccggtac tgcggatatg ttcaatacgc tgaagctgct gaccgttgtc 660
atgtggctcg gctaccccat cgtgtgggca ctcggcgttg agggcatcgc cgttcttccg
720 gtcggagtca cgtcgtgggg atacagcttc ctcgacatcg tcgcgaagta
catcttcgcg 780 ttcctgctgc tcaactacct cacgtcgaac gagagcgtcg
tctccggctc gatactcgac 840 gtgccgtccg cgtcgggcac tcccgctgac gac 873
<210> SEQ ID NO 24 <400> SEQUENCE: 24 000 <210>
SEQ ID NO 25 <211> LENGTH: 708 <212> TYPE: DNA
<213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: GFP <400> SEQUENCE: 25
aaaggagaag aactcttcac tggagttgtc ccaattcttg ttgaattaga tggtgatgtt
60 aacggccaca agttctctgt cagtggagag ggtgaaggtg atgcaacata
cggaaaactt 120 accctgaagt tcatctgcac tactggcaaa ctgcctgttc
catggccaac actagtcact 180 actctgtgct atggtgttca atgcttttca
agatacccgg atcatatgaa acggcatgac 240 tttttcaaga gtgccatgcc
cgaaggttat gtacaggaaa ggaccatctt cttcaaagat 300 gacggcaact
acaagacacg tgctgaagtc aagtttgaag gtgataccct tgttaataga 360
atcgagttaa aaggtattga cttcaaggaa gatggcaaca ttctgggaca caaattggaa
420 tacaactata actcacacaa tgtatacatc atggcagaca aacaaaagaa
tggaatcaaa 480 gtgaacttca agacccgcca caacattgaa gatggaagcg
ttcaactagc agaccattat 540 caacaaaata ctccaattgg cgatggccct
gtccttttac cagacaacca ttacctgtcc 600 acacaatctg ccctttcgaa
agatcccaac gaaaagagag accacatggt ccttcttgag 660 tttgtaacag
ctgctgggat tacacatggc atggatgaac tgtacaac 708 <210> SEQ ID NO
26 <211> LENGTH: 972 <212> TYPE: DNA <213>
ORGANISM: Artificial Sequence <220> FEATURE: <223>
OTHER INFORMATION: CAG promoter <400> SEQUENCE: 26 ctagttatta
atagtaatca attacggggt cattagttca tagcccatat atggagttcc 60
gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat
120 tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
cattgacgtc 180 aatgggtgga gtatttacgg taaactgccc acttggcagt
acatcaagtg tatcatatgc 240 caagtacgcc ccctattgac gtcaatgacg
gtaaatggcc cgcctggcat tatgcccagt 300 acatgacctt atgggacttt
cctacttggc agtacatcta cgtattagtc atcgctatta 360 ccatgcatgg
tcgaggtgag ccccacgttc tgcttcactc tccccatctc ccccccctcc 420
ccacccccaa ttttgtattt atttattttt taattatttt gtgcagcgat gggggcgggg
480 gggggggggg ggcgcgcgcc aggcggggcg gggcggggcg aggggcgggg
cggggcgagg 540 cggagaggtg cggcggcagc caatcagagc ggcgcgctcc
gaaagtttcc ttttatggcg 600 aggcggcggc ggcggcggcc ctataaaaag
cgaagcgcgc ggcgggcggg agtcgctgcg 660 acgctgcctt cgccccgtgc
cccgctccgc cgccgcctcg cgccgcccgc cccggctctg 720 actgaccgcg
ttactcccac aggtgagcgg gcgggacggc ccttctcctc cgggctgtaa 780
ttagcgcttg gtttaatgac ggcttgtttc ttttctgtgg ctgcgtgaaa gccttgaggg
840 gctccgggag ggccctttgt gcggggggag cggctcgggg ctgtccgcgg
ggggacggct 900 gccttcgggg gggacggggc agggcggggt tcggcttctg
gcgtgtgacc ggcggctcta 960 gcagcctctg ct 972 <210> SEQ ID NO
27 <211> LENGTH: 597 <212> TYPE: DNA <213>
ORGANISM: Artificial Sequence <220> FEATURE: <223>
OTHER INFORMATION: Posttranscriptional regulatory element WPRE
<400> SEQUENCE: 27 gataatcaac ctctggatta caaaatttgt
gaaagattga ctggtattct taactatgtt 60 gctcctttta cgctatgtgg
atacgctgct ttaatgcctt tgtatcatgc tattgcttcc 120 cgtatggctt
tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag 180
ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
240 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
tttccccctc 300 cctattgcca cggcggaact catcgccgcc tgccttgccc
gctgctggac aggggctcgg 360 ctgttgggca ctgacaattc cgtggtgttg
tcggggaagc tgacgtcctt tccatggctg 420 ctcgcctgtg ttgccacctg
gattctgcgc gggacgtcct tctgctacgt cccttcggcc 480 ctcaatccag
cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt 540
cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctgat 597
<210> SEQ ID NO 28 <211> LENGTH: 479 <212> TYPE:
DNA <213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: Polyadenylation sequence <400>
SEQUENCE: 28 acgggtggca tccctgtgac ccctccccag tgcctctcct ggccctggaa
gttgccactc 60 cagtgcccac cagccttgtc ctaataaaat taagttgcat
cattttgtct gactaggtgt 120 ccttctataa tattatgggg tggagggggg
tggtatggag caaggggcaa gttgggaaga 180 caacctgtag ggcctgcggg
gtctattggg aaccaagctg gagtgcagtg gcacaatctt 240 ggctcactgc
aatctccgcc tcctgggttc aagcgattct cctgcctcag cctcccgagt 300
tgttgggatt ccaggcatgc atgaccaggc tcagctaatt tttgtttttt tggtagagac
360 ggggtttcac catattggcc aggctggtct ccaactccta atctcaggtg
atctacccac 420 cttggcctcc caaattgctg ggattacagg cgtgaaccac
tgctcccttc cctgtcctt 479 <210> SEQ ID NO 29 <400>
SEQUENCE: 29 000 <210> SEQ ID NO 30 <211> LENGTH: 4232
<212> TYPE: DNA <213> ORGANISM: Artificial Sequence
<220> FEATURE: <223> OTHER INFORMATION: 5'
-ITR-CAG-ChR2-GFP-WPRE-bGHpolyA-ITR- 3' <400> SEQUENCE: 30
cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
60 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag
ggagtggcca 120 actccatcac taggggttcc tgcggccgca cgcgtgatat
cctagttatt aatagtaatc 180 aattacgggg tcattagttc atagcccata
tatggagttc cgcgttacat aacttacggt 240 aaatggcccg cctggctgac
cgcccaacga cccccgccca ttgacgtcaa taatgacgta 300 tgttcccata
gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg 360
gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
420 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
tatgggactt 480 tcctacttgg cagtacatct acgtattagt catcgctatt
accatgcatg gtcgaggtga 540 gccccacgtt ctgcttcact ctccccatct
cccccccctc cccaccccca attttgtatt 600 tatttatttt ttaattattt
tgtgcagcga tgggggcggg gggggggggg gggcgcgcgc 660 caggcggggc
ggggcggggc gaggggcggg gcggggcgag gcggagaggt gcggcggcag 720
ccaatcagag cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg cggcggcggc
780 cctataaaaa gcgaagcgcg cggcgggcgg gagtcgctgc gacgctgcct
tcgccccgtg 840 ccccgctccg ccgccgcctc gcgccgcccg ccccggctct
gactgaccgc gttactccca 900 caggtgagcg ggcgggacgg cccttctcct
ccgggctgta attagcgctt ggtttaatga 960 cggcttgttt cttttctgtg
gctgcgtgaa agccttgagg ggctccggga gggccctttg 1020 tgcgggggga
gcggctcggg gctgtccgcg gggggacggc tgccttcggg ggggacgggg 1080
cagggcgggg ttcggcttct ggcgtgtgac cggcggctct agcagcctct gctaaccatg
1140 ttcatgcctt cttctttttc ctacagctcc tgggcaacgt gctggttatt
gtgctgtctc 1200 atcattttgg caaagaatta agcttgagct cgcgatccgc
agccatggat tatggaggcg 1260 ccctgagtgc cgttgggcgc gagctgctat
ttgtaacgaa cccagtagtc gtcaatggct 1320 ctgtacttgt gcctgaggac
cagtgttact gcgcgggctg gattgagtcg cgtggcacaa 1380 acggtgccca
aacggcgtcg aacgtgctgc aatggcttgc tgctggcttc tccatcctac 1440
tgcttatgtt ttacgcctac caaacatgga agtcaacctg cggctgggag gagatctatg
1500 tgtgcgctat cgagatggtc aaggtgattc ttgagttctt cttcgagttt
aagaacccgt 1560 ccatgctgta tctagccaca ggccaccgcg tccagtggtt
gcgttacgcc gagtggcttc 1620 tcacctgccc ggtcattctc attcacctgt
caaacctgac gggcttgtcc aacgactaca 1680 gcaggcgcac tatgggtctg
cttgtgtctg atattggcac aattgtgtgg ggcgccactt 1740 ccgctatggc
caccggatac gtcaaggtca tcttcttctg cctgggtctg tgttatggtg 1800
ctaacacgtt ctttcacgct gccaaggcct acatcgaggg ttaccatacc gtgccgaagg
1860 gccggtgtcg ccaggtggtg actggcatgg cttggctctt cttcgtatca
tggggtatgt 1920 tccccatcct gttcatcctc ggccccgagg gcttcggcgt
cctgagcgtg tacggctcca 1980 ccgtcggcca caccatcatt gacctgatgt
cgaagaactg ctggggtctg ctcggccact 2040 acctgcgcgt gctgatccac
gagcatatcc tcatccacgg cgacattcgc aagaccacca 2100 aattgaacat
tggtggcact gagattgagg tcgagacgct ggtggaggac gaggccgagg 2160
ctggcgcggt caacaagggc accggcaagg aattcggagg cggaggtgga gctagcaaag
2220 gagaagaact cttcactgga gttgtcccaa ttcttgttga attagatggt
gatgttaacg 2280 gccacaagtt ctctgtcagt ggagagggtg aaggtgatgc
aacatacgga aaacttaccc 2340 tgaagttcat ctgcactact ggcaaactgc
ctgttccatg gccaacacta gtcactactc 2400 tgtgctatgg tgttcaatgc
ttttcaagat acccggatca tatgaaacgg catgactttt 2460 tcaagagtgc
catgcccgaa ggttatgtac aggaaaggac catcttcttc aaagatgacg 2520
gcaactacaa gacacgtgct gaagtcaagt ttgaaggtga tacccttgtt aatagaatcg
2580 agttaaaagg tattgacttc aaggaagatg gcaacattct gggacacaaa
ttggaataca 2640 actataactc acacaatgta tacatcatgg cagacaaaca
aaagaatgga atcaaagtga 2700 acttcaagac ccgccacaac attgaagatg
gaagcgttca actagcagac cattatcaac 2760 aaaatactcc aattggcgat
ggccctgtcc ttttaccaga caaccattac ctgtccacac 2820 aatctgccct
ttcgaaagat cccaacgaaa agagagacca catggtcctt cttgagtttg 2880
taacagctgc tgggattaca catggcatgg atgaactgta caactaactc gagtctagac
2940 gtggtaccga taatcaacct ctggattaca aaatttgtga aagattgact
ggtattctta 3000 actatgttgc tccttttacg ctatgtggat acgctgcttt
aatgcctttg tatcatgcta 3060 ttgcttcccg tatggctttc attttctcct
ccttgtataa atcctggttg ctgtctcttt 3120 atgaggagtt gtggcccgtt
gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg 3180 caacccccac
tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt 3240
tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
3300 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaagctg
acgtcctttc 3360 catggctgct cgcctgtgtt gccacctgga ttctgcgcgg
gacgtccttc tgctacgtcc 3420 cttcggccct caatccagcg gaccttcctt
cccgcggcct gctgccggct ctgcggcctc 3480 ttccgcgtct tcgccttcgc
cctcagacga gtcggatctc cctttgggcc gcctccccgc 3540 ctgatgcggg
gatcctctag agtcgagaga tctacgggtg gcatccctgt gacccctccc 3600
cagtgcctct cctggccctg gaagttgcca ctccagtgcc caccagcctt gtcctaataa
3660 aattaagttg catcattttg tctgactagg tgtccttcta taatattatg
gggtggaggg 3720 gggtggtatg gagcaagggg caagttggga agacaacctg
tagggcctgc ggggtctatt 3780 gggaaccaag ctggagtgca gtggcacaat
cttggctcac tgcaatctcc gcctcctggg 3840 ttcaagcgat tctcctgcct
cagcctcccg agttgttggg attccaggca tgcatgacca 3900 ggctcagcta
atttttgttt ttttggtaga gacggggttt caccatattg gccaggctgg 3960
tctccaactc ctaatctcag gtgatctacc caccttggcc tcccaaattg ctgggattac
4020 aggcgtgaac cactgctccc ttccctgtcc ttctgatttt gtaggtaacc
acgtgcggac 4080 cgagcggccg caggaacccc tagtgatgga gttggccact
ccctctctgc gcgctcgctc 4140 gctcactgag gccgggcgac caaaggtcgc
ccgacgcccg ggctttgccc gggcggcctc 4200 agtgagcgag cgagcgcgca
gctgcctgca gg 4232 <210> SEQ ID NO 31 <211> LENGTH:
3488 <212> TYPE: DNA <213> ORGANISM: Artificial
Sequence <220> FEATURE: <223> OTHER INFORMATION: 5'
-ITR-CAG-ChR2-WPRE-bGHpolyA-ITR- 3' <400> SEQUENCE: 31
cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
60 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag
ggagtggcca 120 actccatcac taggggttcc tgcggccgca cgcgtgatat
cctagttatt aatagtaatc 180 aattacgggg tcattagttc atagcccata
tatggagttc cgcgttacat aacttacggt 240 aaatggcccg cctggctgac
cgcccaacga cccccgccca ttgacgtcaa taatgacgta 300 tgttcccata
gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg 360
gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
420 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
tatgggactt 480 tcctacttgg cagtacatct acgtattagt catcgctatt
accatgcatg gtcgaggtga 540 gccccacgtt ctgcttcact ctccccatct
cccccccctc cccaccccca attttgtatt 600 tatttatttt ttaattattt
tgtgcagcga tgggggcggg gggggggggg gggcgcgcgc 660 caggcggggc
ggggcggggc gaggggcggg gcggggcgag gcggagaggt gcggcggcag 720
ccaatcagag cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg cggcggcggc
780 cctataaaaa gcgaagcgcg cggcgggcgg gagtcgctgc gacgctgcct
tcgccccgtg 840 ccccgctccg ccgccgcctc gcgccgcccg ccccggctct
gactgaccgc gttactccca 900 caggtgagcg ggcgggacgg cccttctcct
ccgggctgta attagcgctt ggtttaatga 960 cggcttgttt cttttctgtg
gctgcgtgaa agccttgagg ggctccggga gggccctttg 1020 tgcgggggga
gcggctcggg gctgtccgcg gggggacggc tgccttcggg ggggacgggg 1080
cagggcgggg ttcggcttct ggcgtgtgac cggcggctct agcagcctct gctaaccatg
1140 ttcatgcctt cttctttttc ctacagctcc tgggcaacgt gctggttatt
gtgctgtctc 1200 atcattttgg caaagaatta agcttgagct cgcgatccgc
agccatggat tatggaggcg 1260 ccctgagtgc cgttgggcgc gagctgctat
ttgtaacgaa cccagtagtc gtcaatggct 1320 ctgtacttgt gcctgaggac
cagtgttact gcgcgggctg gattgagtcg cgtggcacaa 1380 acggtgccca
aacggcgtcg aacgtgctgc aatggcttgc tgctggcttc tccatcctac 1440
tgcttatgtt ttacgcctac caaacatgga agtcaacctg cggctgggag gagatctatg
1500 tgtgcgctat cgagatggtc aaggtgattc ttgagttctt cttcgagttt
aagaacccgt 1560 ccatgctgta tctagccaca ggccaccgcg tccagtggtt
gcgttacgcc gagtggcttc 1620 tcacctgccc ggtcattctc attcacctgt
caaacctgac gggcttgtcc aacgactaca 1680 gcaggcgcac tatgggtctg
cttgtgtctg atattggcac aattgtgtgg ggcgccactt 1740 ccgctatggc
caccggatac gtcaaggtca tcttcttctg cctgggtctg tgttatggtg 1800
ctaacacgtt ctttcacgct gccaaggcct acatcgaggg ttaccatacc gtgccgaagg
1860 gccggtgtcg ccaggtggtg actggcatgg cttggctctt cttcgtatca
tggggtatgt 1920 tccccatcct gttcatcctc ggccccgagg gcttcggcgt
cctgagcgtg tacggctcca 1980 ccgtcggcca caccatcatt gacctgatgt
cgaagaactg ctggggtctg ctcggccact 2040 acctgcgcgt gctgatccac
gagcatatcc tcatccacgg cgacattcgc aagaccacca 2100 aattgaacat
tggtggcact gagattgagg tcgagacgct ggtggaggac gaggccgagg 2160
ctggcgcggt caacaagggc accggcaagg aattcggagg cggaggtgga gctagctaac
2220 tcgagtctag acgtggtacc ttgactggta ttcttaacta tgttgctcct
tttacgctat 2280 gtggatacgc tgctttaatg cctttgtatc atgctattgc
ttcccgtatg gctttcattt 2340 tctcctcctt gtataaatcc tggttgctgt
ctctttatga ggagttgtgg cccgttgtca 2400 ggcaacgtgg cgtggtgtgc
actgtgtttg ctgacgcaac ccccactggt tggggcattg 2460 ccaccacctg
tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg 2520
aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
2580 attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc
tgtgttgcca 2640 cctggattct gcgcgggacg tccttctgct acgtcccttc
ggccctcaat ccagcggacc 2700 ttccttcccg cggcctgctg ccggctctgc
ggcctcttcc gcgtcttcgc cttcgccctc 2760 agacgagtcg gatctccctt
tgggccgcct ccccgcctga tgcggggatc ctctagagtc 2820 gagagatcta
cgggtggcat ccctgtgacc cctccccagt gcctctcctg gccctggaag 2880
ttgccactcc agtgcccacc agccttgtcc taataaaatt aagttgcatc attttgtctg
2940 actaggtgtc cttctataat attatggggt ggaggggggt ggtatggagc
aaggggcaag 3000 ttgggaagac aacctgtagg gcctgcgggg tctattggga
accaagctgg agtgcagtgg 3060 cacaatcttg gctcactgca atctccgcct
cctgggttca agcgattctc ctgcctcagc 3120 ctcccgagtt gttgggattc
caggcatgca tgaccaggct cagctaattt ttgttttttt 3180 ggtagagacg
gggtttcacc atattggcca ggctggtctc caactcctaa tctcaggtga 3240
tctacccacc ttggcctccc aaattgctgg gattacaggc gtgaaccact gctcccttcc
3300 ctgtccttct gattttgtag gtaaccacgt gcggaccgag cggccgcagg
aacccctagt 3360 gatggagttg gccactccct ctctgcgcgc tcgctcgctc
actgaggccg ggcgaccaaa 3420 ggtcgcccga cgcccgggct ttgcccgggc
ggcctcagtg agcgagcgag cgcgcagctg 3480 cctgcagg 3488 <210> SEQ
ID NO 32 <211> LENGTH: 4427 <212> TYPE: DNA <213>
ORGANISM: Artificial Sequence <220> FEATURE: <223>
OTHER INFORMATION: 5'- ITR-CAG-ChR2-GFP-{Kv2.1
Motif}-WPRE-bGHpolyA-ITR- 3' <400> SEQUENCE: 32 cctgcaggca
gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc 60
gggcgaccgg tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
120 actccatcac taggggttcc tgcggccgca cgcgtgatat cctagttatt
aatagtaatc 180 aattacgggg tcattagttc atagcccata tatggagttc
cgcgttacat aacttacggt 240 aaatggcccg cctggctgac cgcccaacga
cccccgccca ttgacgtcaa taatgacgta 300 tgttcccata gtaacgccaa
tagggacttt ccattgacgt caatgggtgg agtatttacg 360 gtaaactgcc
cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga 420
cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
480 tcctacttgg cagtacatct acgtattagt catcgctatt accatgcatg
gtcgaggtga 540 gccccacgtt ctgcttcact ctccccatct cccccccctc
cccaccccca attttgtatt 600 tatttatttt ttaattattt tgtgcagcga
tgggggcggg gggggggggg gggcgcgcgc 660 caggcggggc ggggcggggc
gaggggcggg gcggggcgag gcggagaggt gcggcggcag 720 ccaatcagag
cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg cggcggcggc 780
cctataaaaa gcgaagcgcg cggcgggcgg gagtcgctgc gacgctgcct tcgccccgtg
840 ccccgctccg ccgccgcctc gcgccgcccg ccccggctct gactgaccgc
gttactccca 900 caggtgagcg ggcgggacgg cccttctcct ccgggctgta
attagcgctt ggtttaatga 960 cggcttgttt cttttctgtg gctgcgtgaa
agccttgagg ggctccggga gggccctttg 1020 tgcgggggga gcggctcggg
gctgtccgcg gggggacggc tgccttcggg ggggacgggg 1080 cagggcgggg
ttcggcttct ggcgtgtgac cggcggctct agcagcctct gctaaccatg 1140
ttcatgcctt cttctttttc ctacagctcc tgggcaacgt gctggttatt gtgctgtctc
1200 atcattttgg caaagaatta agcttgagct cgcgatccgc agccatggat
tatggaggcg 1260 ccctgagtgc cgttgggcgc gagctgctat ttgtaacgaa
cccagtagtc gtcaatggct 1320 ctgtacttgt gcctgaggac cagtgttact
gcgcgggctg gattgagtcg cgtggcacaa 1380 acggtgccca aacggcgtcg
aacgtgctgc aatggcttgc tgctggcttc tccatcctac 1440 tgcttatgtt
ttacgcctac caaacatgga agtcaacctg cggctgggag gagatctatg 1500
tgtgcgctat cgagatggtc aaggtgattc ttgagttctt cttcgagttt aagaacccgt
1560 ccatgctgta tctagccaca ggccaccgcg tccagtggtt gcgttacgcc
gagtggcttc 1620 tcacctgccc ggtcattctc attcacctgt caaacctgac
gggcttgtcc aacgactaca 1680 gcaggcgcac tatgggtctg cttgtgtctg
atattggcac aattgtgtgg ggcgccactt 1740 ccgctatggc caccggatac
gtcaaggtca tcttcttctg cctgggtctg tgttatggtg 1800 ctaacacgtt
ctttcacgct gccaaggcct acatcgaggg ttaccatacc gtgccgaagg 1860
gccggtgtcg ccaggtggtg actggcatgg cttggctctt cttcgtatca tggggtatgt
1920 tccccatcct gttcatcctc ggccccgagg gcttcggcgt cctgagcgtg
tacggctcca 1980 ccgtcggcca caccatcatt gacctgatgt cgaagaactg
ctggggtctg ctcggccact 2040 acctgcgcgt gctgatccac gagcatatcc
tcatccacgg cgacattcgc aagaccacca 2100 aattgaacat tggtggcact
gagattgagg tcgagacgct ggtggaggac gaggccgagg 2160 ctggcgcggt
caacaagggc accggcaagg aattcggagg cggaggtgga gctagcaaag 2220
gagaagaact cttcactgga gttgtcccaa ttcttgttga attagatggt gatgttaacg
2280 gccacaagtt ctctgtcagt ggagagggtg aaggtgatgc aacatacgga
aaacttaccc 2340 tgaagttcat ctgcactact ggcaaactgc ctgttccatg
gccaacacta gtcactactc 2400 tgtgctatgg tgttcaatgc ttttcaagat
acccggatca tatgaaacgg catgactttt 2460 tcaagagtgc catgcccgaa
ggttatgtac aggaaaggac catcttcttc aaagatgacg 2520 gcaactacaa
gacacgtgct gaagtcaagt ttgaaggtga tacccttgtt aatagaatcg 2580
agttaaaagg tattgacttc aaggaagatg gcaacattct gggacacaaa ttggaataca
2640 actataactc acacaatgta tacatcatgg cagacaaaca aaagaatgga
atcaaagtga 2700 acttcaagac ccgccacaac attgaagatg gaagcgttca
actagcagac cattatcaac 2760 aaaatactcc aattggcgat ggccctgtcc
ttttaccaga caaccattac ctgtccacac 2820 aatctgccct ttcgaaagat
cccaacgaaa agagagacca catggtcctt cttgagtttg 2880 taacagctgc
tgggattaca catggcatgg atgaactgta caaccagtct cagcccatcc 2940
tgaacactaa ggagatggcc cctcagagta aaccccctga ggaactggaa atgagctcca
3000 tgccatctcc agtggctcct ctgccagcta ggaccgaggg cgtgattgac
atgagaagca 3060 tgtctagtat cgatagcttc atttcctgcg ccaccgactt
ccccgaagct acaaggtttt 3120 aactcgagtc tagacgtggt accgataatc
aacctctgga ttacaaaatt tgtgaaagat 3180 tgactggtat tcttaactat
gttgctcctt ttacgctatg tggatacgct gctttaatgc 3240 ctttgtatca
tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct 3300
ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
3360 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
cagctccttt 3420 ccgggacttt cgctttcccc ctccctattg ccacggcgga
actcatcgcc gcctgccttg 3480 cccgctgctg gacaggggct cggctgttgg
gcactgacaa ttccgtggtg ttgtcgggga 3540 agctgacgtc ctttccatgg
ctgctcgcct gtgttgccac ctggattctg cgcgggacgt 3600 ccttctgcta
cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc 3660
cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt
3720 gggccgcctc cccgcctgat gcggggatcc tctagagtcg agagatctac
gggtggcatc 3780 cctgtgaccc ctccccagtg cctctcctgg ccctggaagt
tgccactcca gtgcccacca 3840 gccttgtcct aataaaatta agttgcatca
ttttgtctga ctaggtgtcc ttctataata 3900 ttatggggtg gaggggggtg
gtatggagca aggggcaagt tgggaagaca acctgtaggg 3960 cctgcggggt
ctattgggaa ccaagctgga gtgcagtggc acaatcttgg ctcactgcaa 4020
tctccgcctc ctgggttcaa gcgattctcc tgcctcagcc tcccgagttg ttgggattcc
4080 aggcatgcat gaccaggctc agctaatttt tgtttttttg gtagagacgg
ggtttcacca 4140 tattggccag gctggtctcc aactcctaat ctcaggtgat
ctacccacct tggcctccca 4200 aattgctggg attacaggcg tgaaccactg
ctcccttccc tgtccttctg attttgtagg 4260 taaccacgtg cggaccgagc
ggccgcagga acccctagtg atggagttgg ccactccctc 4320 tctgcgcgct
cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac gcccgggctt 4380
tgcccgggcg gcctcagtga gcgagcgagc gcgcagctgc ctgcagg 4427
<210> SEQ ID NO 33 <211> LENGTH: 3719 <212> TYPE:
DNA <213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: 5' -ITR-CAG-ChR2-{Kv2 .1
Motif}-WPRE-bGHpolyA-ITR- 3' <400> SEQUENCE: 33 cctgcaggca
gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc 60
gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
120 actccatcac taggggttcc tgcggccgca cgcgtgatat cctagttatt
aatagtaatc 180 aattacgggg tcattagttc atagcccata tatggagttc
cgcgttacat aacttacggt 240 aaatggcccg cctggctgac cgcccaacga
cccccgccca ttgacgtcaa taatgacgta 300 tgttcccata gtaacgccaa
tagggacttt ccattgacgt caatgggtgg agtatttacg 360 gtaaactgcc
cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga 420
cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
480 tcctacttgg cagtacatct acgtattagt catcgctatt accatgcatg
gtcgaggtga 540 gccccacgtt ctgcttcact ctccccatct cccccccctc
cccaccccca attttgtatt 600 tatttatttt ttaattattt tgtgcagcga
tgggggcggg gggggggggg gggcgcgcgc 660 caggcggggc ggggcggggc
gaggggcggg gcggggcgag gcggagaggt gcggcggcag 720 ccaatcagag
cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg cggcggcggc 780
cctataaaaa gcgaagcgcg cggcgggcgg gagtcgctgc gacgctgcct tcgccccgtg
840 ccccgctccg ccgccgcctc gcgccgcccg ccccggctct gactgaccgc
gttactccca 900 caggtgagcg ggcgggacgg cccttctcct ccgggctgta
attagcgctt ggtttaatga 960 cggcttgttt cttttctgtg gctgcgtgaa
agccttgagg ggctccggga gggccctttg 1020 tgcgggggga gcggctcggg
gctgtccgcg gggggacggc tgccttcggg ggggacgggg 1080 cagggcgggg
ttcggcttct ggcgtgtgac cggcggctct agcagcctct gctaaccatg 1140
ttcatgcctt cttctttttc ctacagctcc tgggcaacgt gctggttatt gtgctgtctc
1200 atcattttgg caaagaatta agcttgagct cgcgatccgc agccatggat
tatggaggcg 1260 ccctgagtgc cgttgggcgc gagctgctat ttgtaacgaa
cccagtagtc gtcaatggct 1320 ctgtacttgt gcctgaggac cagtgttact
gcgcgggctg gattgagtcg cgtggcacaa 1380 acggtgccca aacggcgtcg
aacgtgctgc aatggcttgc tgctggcttc tccatcctac 1440 tgcttatgtt
ttacgcctac caaacatgga agtcaacctg cggctgggag gagatctatg 1500
tgtgcgctat cgagatggtc aaggtgattc ttgagttctt cttcgagttt aagaacccgt
1560 ccatgctgta tctagccaca ggccaccgcg tccagtggtt gcgttacgcc
gagtggcttc 1620 tcacctgccc ggtcattctc attcacctgt caaacctgac
gggcttgtcc aacgactaca 1680 gcaggcgcac tatgggtctg cttgtgtctg
atattggcac aattgtgtgg ggcgccactt 1740 ccgctatggc caccggatac
gtcaaggtca tcttcttctg cctgggtctg tgttatggtg 1800 ctaacacgtt
ctttcacgct gccaaggcct acatcgaggg ttaccatacc gtgccgaagg 1860
gccggtgtcg ccaggtggtg actggcatgg cttggctctt cttcgtatca tggggtatgt
1920 tccccatcct gttcatcctc ggccccgagg gcttcggcgt cctgagcgtg
tacggctcca 1980 ccgtcggcca caccatcatt gacctgatgt cgaagaactg
ctggggtctg ctcggccact 2040 acctgcgcgt gctgatccac gagcatatcc
tcatccacgg cgacattcgc aagaccacca 2100 aattgaacat tggtggcact
gagattgagg tcgagacgct ggtggaggac gaggccgagg 2160 ctggcgcggt
caacaagggc accggcaagg aattcggagg cggaggtgga gctagccagt 2220
ctcagcccat cctgaacact aaggagatgg cccctcagag taaaccccct gaggaactgg
2280 aaatgagctc catgccatct ccagtggctc ctctgccagc taggaccgag
ggcgtgattg 2340 acatgagaag catgtctagt atcgatagct tcatttcctg
cgccaccgac ttccccgaag 2400 ctacaaggtt ttaactcgag tctagacgtg
gtaccgataa tcaacctctg gattacaaaa 2460 tttgtgaaag attgactggt
attcttaact atgttgctcc ttttacgcta tgtggatacg 2520 ctgctttaat
gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct 2580
tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
2640 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
gccaccacct 2700 gtcagctcct ttccgggact ttcgctttcc ccctccctat
tgccacggcg gaactcatcg 2760 ccgcctgcct tgcccgctgc tggacagggg
ctcggctgtt gggcactgac aattccgtgg 2820 tgttgtcggg gaagctgacg
tcctttccat ggctgctcgc ctgtgttgcc acctggattc 2880 tgcgcgggac
gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc 2940
gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
3000 ggatctccct ttgggccgcc tccccgcctg atgcggggat cctctagagt
cgagagatct 3060 acgggtggca tccctgtgac ccctccccag tgcctctcct
ggccctggaa gttgccactc 3120 cagtgcccac cagccttgtc ctaataaaat
taagttgcat cattttgtct gactaggtgt 3180 ccttctataa tattatgggg
tggagggggg tggtatggag caaggggcaa gttgggaaga 3240 caacctgtag
ggcctgcggg gtctattggg aaccaagctg gagtgcagtg gcacaatctt 3300
ggctcactgc aatctccgcc tcctgggttc aagcgattct cctgcctcag cctcccgagt
3360 tgttgggatt ccaggcatgc atgaccaggc tcagctaatt tttgtttttt
tggtagagac 3420 ggggtttcac catattggcc aggctggtct ccaactccta
atctcaggtg atctacccac 3480 cttggcctcc caaattgctg ggattacagg
cgtgaaccac tgctcccttc cctgtccttc 3540 tgattttgta ggtaaccacg
tgcggaccga gcggccgcag gaacccctag tgatagagtt 3600 ggccactccc
tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa aagtcgcccg 3660
acgcccgggc tttgcccggg cggcctcagt gagcgagcga gcgcgcagct gcctgcagg
3719 <210> SEQ ID NO 34 <211> LENGTH: 4313 <212>
TYPE: DNA <213> ORGANISM: Artificial Sequence <220>
FEATURE: <223> OTHER INFORMATION: 5'-ITR-CAG-ChR2-GFP-{Nav1.6
Motif}-WPRE-bGHpolyA-ITR- 3' <400> SEQUENCE: 34 cctgcaggca
gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc 60
gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
120 actccatcac taggggttcc tgcggccgca cgcgtgatat cctagttatt
aatagtaatc 180 aattacgggg tcattagttc atagcccata tatggagttc
cgcgttacat aacttacggt 240 aaatggcccg cctggctgac cgcccaacga
cccccgccca ttgacgtcaa taatgacgta 300 tgttcccata gtaacgccaa
tagggacttt ccattgacgt caatgggtgg agtatttacg 360 gtaaactgcc
cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga 420
cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
480 tcctacttgg cagtacatct acgtattagt catcgctatt accatgcatg
gtcgaggtga 540 gccccacgtt ctgcttcact ctccccatct cccccccctc
cccaccccca attttgtatt 600 tatttatttt ttaattattt tgtgcagcga
tgggggcggg gggggggggg gggcgcgcgc 660 caggcggggc ggggcggggc
gaggggcggg gcggggcgag gcggagaggt gcggcggcag 720 ccaatcagag
cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg cggcggcggc 780
cctataaaaa gcgaagcgcg cggcgggcgg gagtcgctgc gacgctgcct tcgccccgtg
840 ccccgctccg ccgccgcctc gcgccgcccg ccccggctct gactgaccgc
gttactccca 900 caggtgagcg ggcgggacgg cccttctcct ccgggctgta
attagcgctt ggtttaatga 960 cggcttgttt cttttctgtg gctgcgtgaa
agccttgagg ggctccggga gggccctttg 1020 tgcgggggga gcggctcggg
gctgtccgcg gggggacggc tgccttcggg ggggacgggg 1080 cagggcgggg
ttcggcttct ggcgtgtgac cggcggctct agcagcctct gctaaccatg 1140
ttcatgcctt cttctttttc ctacagctcc tgggcaacgt gctggttatt gtgctgtctc
1200 atcattttgg caaagaatta agcttgagct cgcgatccgc agccatggat
tatggaggcg 1260 ccctgagtgc cgttgggcgc gagctgctat ttgtaacgaa
cccagtagtc gtcaatggct 1320 ctgtacttgt gcctgaggac cagtgttact
gcgcgggctg gattgagtcg cgtggcacaa 1380 acggtgccca aacggcgtcg
aacgtgctgc aatggcttgc tgctggcttc tccatcctac 1440 tgcttatgtt
ttacgcctac caaacatgga agtcaacctg cggctgggag gagatctatg 1500
tgtgcgctat cgagatggtc aaggtgattc ttgagttctt cttcgagttt aagaacccgt
1560 ccatgctgta tctagccaca ggccaccgcg tccagtggtt gcgttacgcc
gagtggcttc 1620 tcacctgccc ggtcattctc attcacctgt caaacctgac
gggcttgtcc aacgactaca 1680 gcaggcgcac tatgggtctg cttgtgtctg
atattggcac aattgtgtgg ggcgccactt 1740 ccgctatggc caccggatac
gtcaaggtca tcttcttctg cctgggtctg tgttatggtg 1800 ctaacacgtt
ctttcacgct gccaaggcct acatcgaggg ttaccatacc gtgccgaagg 1860
gccggtgtcg ccaggtggtg actggcatgg cttggctctt cttcgtatca tggggtatgt
1920 tccccatcct gttcatcctc ggccccgagg gcttcggcgt cctgagcgtg
tacggctcca 1980 ccgtcggcca caccatcatt gacctgatgt cgaagaactg
ctggggtctg ctcggccact 2040 acctgcgcgt gctgatccac gagcatatcc
tcatccacgg cgacattcgc aagaccacca 2100 aattgaacat tggtggcact
gagattgagg tcgagacgct ggtggaggac gaggccgagg 2160 ctggcgcggt
caacaagggc accggcaagg aattcggagg cggaggtgga gctagcaaag 2220
gagaagaact cttcactgga gttgtcccaa ttcttgttga attagatggt gatgttaacg
2280 gccacaagtt ctctgtcagt ggagagggtg aaggtgatgc aacatacgga
aaacttaccc 2340 tgaagttcat ctgcactact ggcaaactgc ctgttccatg
gccaacacta gtcactactc 2400 tgtgctatgg tgttcaatgc ttttcaagat
acccggatca tatgaaacgg catgactttt 2460 tcaagagtgc catgcccgaa
ggttatgtac aggaaaggac catcttcttc aaagatgacg 2520 gcaactacaa
gacacgtgct gaagtcaagt ttgaaggtga tacccttgtt aatagaatcg 2580
agttaaaagg tattgacttc aaggaagatg gcaacattct gggacacaaa ttggaataca
2640 actataactc acacaatgta tacatcatgg cagacaaaca aaagaatgga
atcaaagtga 2700 acttcaagac ccgccacaac attgaagatg gaagcgttca
actagcagac cattatcaac 2760 aaaatactcc aattggcgat ggccctgtcc
ttttaccaga caaccattac ctgtccacac 2820 aatctgccct ttcgaaagat
cccaacgaaa agagagacca catggtcctt cttgagtttg 2880 taacagctgc
tgggattaca catggcatgg atgaactgta caacaccgtg agggtgccca 2940
tcgccgtggg cgagagcgac ttcgagaacc tgaacaccga ggacgtgagc agcgagagcg
3000 acccctaact cgagtctaga cgtggtaccg ataatcaacc tctggattac
aaaatttgtg 3060 aaagattgac tggtattctt aactatgttg ctccttttac
gctatgtgga tacgctgctt 3120 taatgccttt gtatcatgct attgcttccc
gtatggcttt cattttctcc tccttgtata 3180 aatcctggtt gctgtctctt
tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg 3240 tgtgcactgt
gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc 3300
tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
3360 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
gtggtgttgt 3420 cggggaagct gacgtccttt ccatggctgc tcgcctgtgt
tgccacctgg attctgcgcg 3480 ggacgtcctt ctgctacgtc ccttcggccc
tcaatccagc ggaccttcct tcccgcggcc 3540 tgctgccggc tctgcggcct
cttccgcgtc ttcgccttcg ccctcagacg agtcggatct 3600 ccctttgggc
cgcctccccg cctgatgcgg ggatcctcta gagtcgagag atctacgggt 3660
ggcatccctg tgacccctcc ccagtgcctc tcctggccct ggaagttgcc actccagtgc
3720 ccaccagcct tgtcctaata aaattaagtt gcatcatttt gtctgactag
gtgtccttct 3780 ataatattat ggggtggagg ggggtggtat ggagcaaggg
gcaagttggg aagacaacct 3840 gtagggcctg cggggtctat tgggaaccaa
gctggagtgc agtggcacaa tcttggctca 3900 ctgcaatctc cgcctcctgg
gttcaagcga ttctcctgcc tcagcctccc gagttgttgg 3960 gattccaggc
atgcatgacc aggctcagct aatttttgtt tttttggtag agacggggtt 4020
tcaccatatt ggccaggctg gtctccaact cctaatctca ggtgatctac ccaccttggc
4080 ctcccaaatt gctgggatta caggcgtgaa ccactgctcc cttccctgtc
cttctgattt 4140 tgtaggtaac cacgtgcgga ccgagcggcc gcaggaaccc
ctagtgatgg agttggccac 4200 tccctctctg cgcgctcgct cgctcactga
ggccgggcga ccaaaggtcg cccgacgccc 4260 gggctttgcc cgggcggcct
cagtgagcga gcgagcgcgc agctgcctgc agg 4313 <210> SEQ ID NO 35
<211> LENGTH: 3605 <212> TYPE: DNA <213>
ORGANISM: Artificial Sequence <220> FEATURE: <223>
OTHER INFORMATION: 5'-
ITR-CAG-ChR2-{Nav1.6Motif}-WPRE-bGHpolyA-ITR-3' <400>
SEQUENCE: 35 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag
cccgggcgtc 60 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc
gcgcagagag ggagtggcca 120 actccatcac taggggttcc tgcggccgca
cgcgtgatat cctagttatt aatagtaatc 180 aattacgggg tcattagttc
atagcccata tatggagttc cgcgttacat aacttacggt 240 aaatggcccg
cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta 300
tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
360 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
cccctattga 420 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
tacatgacct tatgggactt 480 tcctacttgg cagtacatct acgtattagt
catcgctatt accatgcatg gtcgaggtga 540 gccccacgtt ctgcttcact
ctccccatct cccccccctc cccaccccca attttgtatt 600 tatttatttt
ttaattattt tgtgcagcga tgggggcggg gggggggggg gggcgcgcgc 660
caggcggggc ggggcggggc gaggggcggg gcggggcgag gcggagaggt gcggcggcag
720 ccaatcagag cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg
cggcggcggc 780 cctataaaaa gcgaagcgcg cggcgggcgg gagtcgctgc
gacgctgcct tcgccccgtg 840 ccccgctccg ccgccgcctc gcgccgcccg
ccccggctct gactgaccgc gttactccca 900 caggtgagcg ggcgggacgg
cccttctcct ccgggctgta attagcgctt ggtttaatga 960 cggcttgttt
cttttctgtg gctgcgtgaa agccttgagg ggctccggga gggccctttg 1020
tgcgggggga gcggctcggg gctgtccgcg gggggacggc tgccttcggg ggggacgggg
1080 cagggcgggg ttcggcttct ggcgtgtgac cggcggctct agcagcctct
gctaaccatg 1140 ttcatgcctt cttctttttc ctacagctcc tgggcaacgt
gctggttatt gtgctgtctc 1200 atcattttgg caaagaatta agcttgagct
cgcgatccgc agccatggat tatggaggcg 1260 ccctgagtgc cgttgggcgc
gagctgctat ttgtaacgaa cccagtagtc gtcaatggct 1320 ctgtacttgt
gcctgaggac cagtgttact gcgcgggctg gattgagtcg cgtggcacaa 1380
acggtgccca aacggcgtcg aacgtgctgc aatggcttgc tgctggcttc tccatcctac
1440 tgcttatgtt ttacgcctac caaacatgga agtcaacctg cggctgggag
gagatctatg 1500 tgtgcgctat cgagatggtc aaggtgattc ttgagttctt
cttcgagttt aagaacccgt 1560 ccatgctgta tctagccaca ggccaccgcg
tccagtggtt gcgttacgcc gagtggcttc 1620 tcacctgccc ggtcattctc
attcacctgt caaacctgac gggcttgtcc aacgactaca 1680 gcaggcgcac
tatgggtctg cttgtgtctg atattggcac aattgtgtgg ggcgccactt 1740
ccgctatggc caccggatac gtcaaggtca tcttcttctg cctgggtctg tgttatggtg
1800 ctaacacgtt ctttcacgct gccaaggcct acatcgaggg ttaccatacc
gtgccgaagg 1860 gccggtgtcg ccaggtggtg actggcatgg cttggctctt
cttcgtatca tggggtatgt 1920 tccccatcct gttcatcctc ggccccgagg
gcttcggcgt cctgagcgtg tacggctcca 1980 ccgtcggcca caccatcatt
gacctgatgt cgaagaactg ctggggtctg ctcggccact 2040 acctgcgcgt
gctgatccac gagcatatcc tcatccacgg cgacattcgc aagaccacca 2100
aattgaacat tggtggcact gagattgagg tcgagacgct ggtggaggac gaggccgagg
2160 ctggcgcggt caacaagggc accggcaagg aattcggagg cggaggtgga
gctagcaccg 2220 tgagggtgcc catcgccgtg ggcgagagcg acttcgagaa
cctgaacacc gaggacgtga 2280 gcagcgagag cgacccctaa ctcgagtcta
gacgtggtac cgataatcaa cctctggatt 2340 acaaaatttg tgaaagattg
actggtattc ttaactatgt tgctcctttt acgctatgtg 2400 gatacgctgc
tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct 2460
cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc
2520 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
ggcattgcca 2580 ccacctgtca gctcctttcc gggactttcg ctttccccct
ccctattgcc acggcggaac 2640 tcatcgccgc ctgccttgcc cgctgctgga
caggggctcg gctgttgggc actgacaatt 2700 ccgtggtgtt gtcggggaag
ctgacgtcct ttccatggct gctcgcctgt gttgccacct 2760 ggattctgcg
cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc 2820
cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga
2880 cgagtcggat ctccctttgg gccgcctccc cgcctgatgc ggggatcctc
tagagtcgag 2940 agatctacgg gtggcatccc tgtgacccct ccccagtgcc
tctcctggcc ctggaagttg 3000 ccactccagt gcccaccagc cttgtcctaa
taaaattaag ttgcatcatt ttgtctgact 3060 aggtgtcctt ctataatatt
atggggtgga ggggggtggt atggagcaag gggcaagttg 3120 ggaagacaac
ctgtagggcc tgcggggtct attgggaacc aagctggagt gcagtggcac 3180
aatcttggct cactgcaatc tccgcctcct gggttcaagc gattctcctg cctcagcctc
3240 ccgagttgtt gggattccag gcatgcatga ccaggctcag ctaatttttg
tttttttggt 3300 agagacgggg tttcaccata ttggccaggc tggtctccaa
ctcctaatct caggtgatct 3360 acccaccttg gcctcccaaa ttgctgggat
tacaggcgtg aaccactgct cccttccctg 3420 tccttctgat tttgtaggta
accacgtgcg gaccgagcgg ccgcaggaac ccctagtgat 3480 ggagttggcc
actccctctc tgcgcgctcg ctcgctcact gaggccgggc gaccaaaggt 3540
cgcccgacgc ccgggctttg cccgggcggc ctcagtgagc gagcgagcgc gcagctgcct
3600 gcagg 3605 <210> SEQ ID NO 36 <211> LENGTH: 4328
<212> TYPE: DNA <213> ORGANISM: Artificial Sequence
<220> FEATURE: <223> OTHER INFORMATION: 5'-
ITR-CAG-ChR2-GFP-{NLG1 Motif}-WPRE-bGHpolyA-ITR- 3' <400>
SEQUENCE: 36 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag
cccgggcgtc 60 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc
gcgcagagag ggagtggcca 120 actccatcac taggggttcc tgcggccgca
cgcgtgatat cctagttatt aatagtaatc 180 aattacgggg tcattagttc
atagcccata tatggagttc cgcgttacat aacttacggt 240 aaatggcccg
cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta 300
tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
360 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
cccctattga 420 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
tacatgacct tatgggactt 480 tcctacttgg cagtacatct acgtattagt
catcgctatt accatgcatg gtcgaggtga 540 gccccacgtt ctgcttcact
ctccccatct cccccccctc cccaccccca attttgtatt 600 tatttatttt
ttaattattt tgtgcagcga tgggggcggg gggggggggg gggcgcgcgc 660
caggcggggc ggggcggggc gaggggcggg gcggggcgag gcggagaggt gcggcggcag
720 ccaatcagag cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg
cggcggcggc 780 cctataaaaa gcgaagcgcg cggcgggcgg gagtcgctgc
gacgctgcct tcgccccgtg 840 ccccgctccg ccgccgcctc gcgccgcccg
ccccggctct gactgaccgc gttactccca 900 caggtgagcg ggcgggacgg
cccttctcct ccgggctgta attagcgctt ggtttaatga 960 cggcttgttt
cttttctgtg gctgcgtgaa agccttgagg ggctccggga gggccctttg 1020
tgcgggggga gcggctcggg gctgtccgcg gggggacggc tgccttcggg ggggacgggg
1080 cagggcgggg ttcggcttct ggcgtgtgac cggcggctct agcagcctct
gctaaccatg 1140 ttcatgcctt cttctttttc ctacagctcc tgggcaacgt
gctggttatt gtgctgtctc 1200 atcattttgg caaagaatta agcttgagct
cgcgatccgc agccatggat tatggaggcg 1260 ccctgagtgc cgttgggcgc
gagctgctat ttgtaacgaa cccagtagtc gtcaatggct 1320 ctgtacttgt
gcctgaggac cagtgttact gcgcgggctg gattgagtcg cgtggcacaa 1380
acggtgccca aacggcgtcg aacgtgctgc aatggcttgc tgctggcttc tccatcctac
1440 tgcttatgtt ttacgcctac caaacatgga agtcaacctg cggctgggag
gagatctatg 1500 tgtgcgctat cgagatggtc aaggtgattc ttgagttctt
cttcgagttt aagaacccgt 1560 ccatgctgta tctagccaca ggccaccgcg
tccagtggtt gcgttacgcc gagtggcttc 1620 tcacctgccc ggtcattctc
attcacctgt caaacctgac gggcttgtcc aacgactaca 1680 gcaggcgcac
tatgggtctg cttgtgtctg atattggcac aattgtgtgg ggcgccactt 1740
ccgctatggc caccggatac gtcaaggtca tcttcttctg cctgggtctg tgttatggtg
1800 ctaacacgtt ctttcacgct gccaaggcct acatcgaggg ttaccatacc
gtgccgaagg 1860 gccggtgtcg ccaggtggtg actggcatgg cttggctctt
cttcgtatca tggggtatgt 1920 tccccatcct gttcatcctc ggccccgagg
gcttcggcgt cctgagcgtg tacggctcca 1980 ccgtcggcca caccatcatt
gacctgatgt cgaagaactg ctggggtctg ctcggccact 2040 acctgcgcgt
gctgatccac gagcatatcc tcatccacgg cgacattcgc aagaccacca 2100
aattgaacat tggtggcact gagattgagg tcgagacgct ggtggaggac gaggccgagg
2160 ctggcgcggt caacaagggc accggcaagg aattcggagg cggaggtgga
gctagcaaag 2220 gagaagaact cttcactgga gttgtcccaa ttcttgttga
attagatggt gatgttaacg 2280 gccacaagtt ctctgtcagt ggagagggtg
aaggtgatgc aacatacgga aaacttaccc 2340 tgaagttcat ctgcactact
ggcaaactgc ctgttccatg gccaacacta gtcactactc 2400 tgtgctatgg
tgttcaatgc ttttcaagat acccggatca tatgaaacgg catgactttt 2460
tcaagagtgc catgcccgaa ggttatgtac aggaaaggac catcttcttc aaagatgacg
2520 gcaactacaa gacacgtgct gaagtcaagt ttgaaggtga tacccttgtt
aatagaatcg 2580 agttaaaagg tattgacttc aaggaagatg gcaacattct
gggacacaaa ttggaataca 2640 actataactc acacaatgta tacatcatgg
cagacaaaca aaagaatgga atcaaagtga 2700 acttcaagac ccgccacaac
attgaagatg gaagcgttca actagcagac cattatcaac 2760 aaaatactcc
aattggcgat ggccctgtcc ttttaccaga caaccattac ctgtccacac 2820
aatctgccct ttcgaaagat cccaacgaaa agagagacca catggtcctt cttgagtttg
2880 taacagctgc tgggattaca catggcatgg atgaactgta caacgtggtt
cttcggaccg 2940 cctgtccccc agattacaca ctagctatga ggaggtcacc
tgatgatgtt cccttaatga 3000 cacccaacac cattacaatg taactcgagt
ctagacgtgg taccgataat caacctctgg 3060 attacaaaat ttgtgaaaga
ttgactggta ttcttaacta tgttgctcct tttacgctat 3120 gtggatacgc
tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt 3180
tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
3240 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
tggggcattg 3300 ccaccacctg tcagctcctt tccgggactt tcgctttccc
cctccctatt gccacggcgg 3360 aactcatcgc cgcctgcctt gcccgctgct
ggacaggggc tcggctgttg ggcactgaca 3420 attccgtggt gttgtcgggg
aagctgacgt cctttccatg gctgctcgcc tgtgttgcca 3480 cctggattct
gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc 3540
ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc
3600 agacgagtcg gatctccctt tgggccgcct ccccgcctga tgcggggatc
ctctagagtc 3660 gagagatcta cgggtggcat ccctgtgacc cctccccagt
gcctctcctg gccctggaag 3720 ttgccactcc agtgcccacc agccttgtcc
taataaaatt aagttgcatc attttgtctg 3780 actaggtgtc cttctataat
attatggggt ggaggggggt ggtatggagc aaggggcaag 3840 ttgggaagac
aacctgtagg gcctgcgggg tctattggga accaagctgg agtgcagtgg 3900
cacaatcttg gctcactgca atctccgcct cctgggttca agcgattctc ctgcctcagc
3960 ctcccgagtt gttgggattc caggcatgca tgaccaggct cagctaattt
ttgttttttt 4020 ggtagagacg gggtttcacc atattggcca ggctggtctc
caactcctaa tctcaggtga 4080 tctacccacc ttggcctccc aaattgctgg
gattacaggc gtgaaccact gctcccttcc 4140 ctgtccttct gattttgtag
gtaaccacgt gcggaccgag cggccgcagg aacccctagt 4200 gatagagttg
gccactccct ctctgcgcgc tcgctcgctc actgaggccg ggcgaccaaa 4260
ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag cgcgcagctg
4320 cctgcagg 4328 <210> SEQ ID NO 37 <211> LENGTH:
3620 <212> TYPE: DNA <213> ORGANISM: Artificial
Sequence <220> FEATURE: <223> OTHER INFORMATION: 5'-
ITR-CAG-ChR2-{NLG-1Motif}-WPRE-bGHpolyA-ITR-3' <400>
SEQUENCE: 37 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag
cccgggcgtc 60 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc
gcgcagagag ggagtggcca 120 actccatcac taggggttcc tgcggccgca
cgcgtgatat cctagttatt aatagtaatc 180 aattacgggg tcattagttc
atagcccata tatggagttc cgcgttacat aacttacggt 240 aaatggcccg
cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta 300
tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
360 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
cccctattga 420 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
tacatgacct tatgggactt 480 tcctacttgg cagtacatct acgtattagt
catcgctatt accatgcatg gtcgaggtga 540 gccccacgtt ctgcttcact
ctccccatct cccccccctc cccaccccca attttgtatt 600 tatttatttt
ttaattattt tgtgcagcga tgggggcggg gggggggggg gggcgcgcgc 660
caggcggggc ggggcggggc gaggggcggg gcggggcgag gcggagaggt gcggcggcag
720 ccaatcagag cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg
cggcggcggc 780 cctataaaaa gcgaagcgcg cggcgggcgg gagtcgctgc
gacgctgcct tcgccccgtg 840 ccccgctccg ccgccgcctc gcgccgcccg
ccccggctct gactgaccgc gttactccca 900 caggtgagcg ggcgggacgg
cccttctcct ccgggctgta attagcgctt ggtttaatga 960 cggcttgttt
cttttctgtg gctgcgtgaa agccttgagg ggctccggga gggccctttg 1020
tgcgggggga gcggctcggg gctgtccgcg gggggacggc tgccttcggg ggggacgggg
1080 cagggcgggg ttcggcttct ggcgtgtgac cggcggctct agcagcctct
gctaaccatg 1140 ttcatgcctt cttctttttc ctacagctcc tgggcaacgt
gctggttatt gtgctgtctc 1200 atcattttgg caaagaatta agcttgagct
cgcgatccgc agccatggat tatggaggcg 1260 ccctgagtgc cgttgggcgc
gagctgctat ttgtaacgaa cccagtagtc gtcaatggct 1320 ctgtacttgt
gcctgaggac cagtgttact gcgcgggctg gattgagtcg cgtggcacaa 1380
acggtgccca aacggcgtcg aacgtgctgc aatggcttgc tgctggcttc tccatcctac
1440 tgcttatgtt ttacgcctac caaacatgga agtcaacctg cggctgggag
gagatctatg 1500 tgtgcgctat cgagatggtc aaggtgattc ttgagttctt
cttcgagttt aagaacccgt 1560 ccatgctgta tctagccaca ggccaccgcg
tccagtggtt gcgttacgcc gagtggcttc 1620 tcacctgccc ggtcattctc
attcacctgt caaacctgac gggcttgtcc aacgactaca 1680 gcaggcgcac
tatgggtctg cttgtgtctg atattggcac aattgtgtgg ggcgccactt 1740
ccgctatggc caccggatac gtcaaggtca tcttcttctg cctgggtctg tgttatggtg
1800 ctaacacgtt ctttcacgct gccaaggcct acatcgaggg ttaccatacc
gtgccgaagg 1860 gccggtgtcg ccaggtggtg actggcatgg cttggctctt
cttcgtatca tggggtatgt 1920 tccccatcct gttcatcctc ggccccgagg
gcttcggcgt cctgagcgtg tacggctcca 1980 ccgtcggcca caccatcatt
gacctgatgt cgaagaactg ctggggtctg ctcggccact 2040 acctgcgcgt
gctgatccac gagcatatcc tcatccacgg cgacattcgc aagaccacca 2100
aattgaacat tggtggcact gagattgagg tcgagacgct ggtggaggac gaggccgagg
2160 ctggcgcggt caacaagggc accggcaagg aattcggagg cggaggtgga
gctagcgtgg 2220 ttcttcggac cgcctgtccc ccagattaca cactagctat
gaggaggtca cctgatgatg 2280 ttcccttaat gacacccaac accattacaa
tgtaactcga gtctagacgt ggtaccgata 2340 atcaacctct ggattacaaa
atttgtgaaa gattgactgg tattcttaac tatgttgctc 2400 cttttacgct
atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta 2460
tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
2520 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
acccccactg 2580 gttggggcat tgccaccacc tgtcagctcc tttccgggac
tttcgctttc cccctcccta 2640 ttgccacggc ggaactcatc gccgcctgcc
ttgcccgctg ctggacaggg gctcggctgt 2700 tgggcactga caattccgtg
gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg 2760 cctgtgttgc
cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca 2820
atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
2880 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct
gatgcgggga 2940 tcctctagag tcgagagatc tacgggtggc atccctgtga
cccctcccca gtgcctctcc 3000 tggccctgga agttgccact ccagtgccca
ccagccttgt cctaataaaa ttaagttgca 3060 tcattttgtc tgactaggtg
tccttctata atattatggg gtggaggggg gtggtatgga 3120 gcaaggggca
agttgggaag acaacctgta gggcctgcgg ggtctattgg gaaccaagct 3180
ggagtgcagt ggcacaatct tggctcactg caatctccgc ctcctgggtt caagcgattc
3240 tcctgcctca gcctcccgag ttgttgggat tccaggcatg catgaccagg
ctcagctaat 3300 ttttgttttt ttggtagaga cggggtttca ccatattggc
caggctggtc tccaactcct 3360 aatctcaggt gatctaccca ccttggcctc
ccaaattgct gggattacag gcgtgaacca 3420 ctgctccctt ccctgtcctt
ctgattttgt aggtaaccac gtgcggaccg agcggccgca 3480 ggaaccccta
gtgatggagt tggccactcc ctctctgcgc gctcgctcgc tcactgaggc 3540
cgggcgacca aaggtcgccc gacgcccggg ctttgcccgg gcggcctcag tgagcgagcg
3600 agcgcgcagc tgcctgcagg 3620 <210> SEQ ID NO 38
<211> LENGTH: 4310 <212> TYPE: DNA <213>
ORGANISM: Artificial Sequence <220> FEATURE: <223>
OTHER INFORMATION: 5'-ITR-CAG-ChR2-GFP- {MLPH
Motif}-WPRE-bGHpolyA-ITR- 3' <400> SEQUENCE: 38 cctgcaggca
gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc 60
gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
120 actccatcac taggggttcc tgcggccgca cgcgtgatat cctagttatt
aatagtaatc 180 aattacgggg tcattagttc atagcccata tatggagttc
cgcgttacat aacttacggt 240 aaatggcccg cctggctgac cgcccaacga
cccccgccca ttgacgtcaa taatgacgta 300 tgttcccata gtaacgccaa
tagggacttt ccattgacgt caatgggtgg agtatttacg 360 gtaaactgcc
cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga 420
cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
480 tcctacttgg cagtacatct acgtattagt catcgctatt accatgcatg
gtcgaggtga 540 gccccacgtt ctgcttcact ctccccatct cccccccctc
cccaccccca attttgtatt 600 tatttatttt ttaattattt tgtgcagcga
tgggggcggg gggggggggg gggcgcgcgc 660 caggcggggc ggggcggggc
gaggggcggg gcggggcgag gcggagaggt gcggcggcag 720 ccaatcagag
cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg cggcggcggc 780
cctataaaaa gcgaagcgcg cggcgggcgg gagtcgctgc gacgctgcct tcgccccgtg
840 ccccgctccg ccgccgcctc gcgccgcccg ccccggctct gactgaccgc
gttactccca 900 caggtgagcg ggcgggacgg cccttctcct ccgggctgta
attagcgctt ggtttaatga 960 cggcttgttt cttttctgtg gctgcgtgaa
agccttgagg ggctccggga gggccctttg 1020 tgcgggggga gcggctcggg
gctgtccgcg gggggacggc tgccttcggg ggggacgggg 1080 cagggcgggg
ttcggcttct ggcgtgtgac cggcggctct agcagcctct gctaaccatg 1140
ttcatgcctt cttctttttc ctacagctcc tgggcaacgt gctggttatt gtgctgtctc
1200 atcattttgg caaagaatta agcttgagct cgcgatccgc agccatggat
tatggaggcg 1260 ccctgagtgc cgttgggcgc gagctgctat ttgtaacgaa
cccagtagtc gtcaatggct 1320 ctgtacttgt gcctgaggac cagtgttact
gcgcgggctg gattgagtcg cgtggcacaa 1380 acggtgccca aacggcgtcg
aacgtgctgc aatggcttgc tgctggcttc tccatcctac 1440 tgcttatgtt
ttacgcctac caaacatgga agtcaacctg cggctgggag gagatctatg 1500
tgtgcgctat cgagatggtc aaggtgattc ttgagttctt cttcgagttt aagaacccgt
1560 ccatgctgta tctagccaca ggccaccgcg tccagtggtt gcgttacgcc
gagtggcttc 1620 tcacctgccc ggtcattctc attcacctgt caaacctgac
gggcttgtcc aacgactaca 1680 gcaggcgcac tatgggtctg cttgtgtctg
atattggcac aattgtgtgg ggcgccactt 1740 ccgctatggc caccggatac
gtcaaggtca tcttcttctg cctgggtctg tgttatggtg 1800 ctaacacgtt
ctttcacgct gccaaggcct acatcgaggg ttaccatacc gtgccgaagg 1860
gccggtgtcg ccaggtggtg actggcatgg cttggctctt cttcgtatca tggggtatgt
1920 tccccatcct gttcatcctc ggccccgagg gcttcggcgt cctgagcgtg
tacggctcca 1980 ccgtcggcca caccatcatt gacctgatgt cgaagaactg
ctggggtctg ctcggccact 2040 acctgcgcgt gctgatccac gagcatatcc
tcatccacgg cgacattcgc aagaccacca 2100 aattgaacat tggtggcact
gagattgagg tcgagacgct ggtggaggac gaggccgagg 2160 ctggcgcggt
caacaagggc accggcaagg aattcggagg cggaggtgga gctagcaaag 2220
gagaagaact cttcactgga gttgtcccaa ttcttgttga attagatggt gatgttaacg
2280 gccacaagtt ctctgtcagt ggagagggtg aaggtgatgc aacatacgga
aaacttaccc 2340 tgaagttcat ctgcactact ggcaaactgc ctgttccatg
gccaacacta gtcactactc 2400 tgtgctatgg tgttcaatgc ttttcaagat
acccggatca tatgaaacgg catgactttt 2460 tcaagagtgc catgcccgaa
ggttatgtac aggaaaggac catcttcttc aaagatgacg 2520 gcaactacaa
gacacgtgct gaagtcaagt ttgaaggtga tacccttgtt aatagaatcg 2580
agttaaaagg tattgacttc aaggaagatg gcaacattct gggacacaaa ttggaataca
2640 actataactc acacaatgta tacatcatgg cagacaaaca aaagaatgga
atcaaagtga 2700 acttcaagac ccgccacaac attgaagatg gaagcgttca
actagcagac cattatcaac 2760 aaaatactcc aattggcgat ggccctgtcc
ttttaccaga caaccattac ctgtccacac 2820 aatctgccct ttcgaaagat
cccaacgaaa agagagacca catggtcctt cttgagtttg 2880 taacagctgc
tgggattaca catggcatgg atgaactgta caacagggac cagcctctga 2940
acagcaaaaa gaaaaagagg ctcctgagct tcagggacgt ggacttcgag gaggacagcg
3000 actaactcga gtctagacgt ggtaccgata atcaacctct ggattacaaa
atttgtgaaa 3060 gattgactgg tattcttaac tatgttgctc cttttacgct
atgtggatac gctgctttaa 3120 tgcctttgta tcatgctatt gcttcccgta
tggctttcat tttctcctcc ttgtataaat 3180 cctggttgct gtctctttat
gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt 3240 gcactgtgtt
tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc 3300
tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
3360 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
gtgttgtcgg 3420 ggaagctgac gtcctttcca tggctgctcg cctgtgttgc
cacctggatt ctgcgcggga 3480 cgtccttctg ctacgtccct tcggccctca
atccagcgga ccttccttcc cgcggcctgc 3540 tgccggctct gcggcctctt
ccgcgtcttc gccttcgccc tcagacgagt cggatctccc 3600 tttgggccgc
ctccccgcct gatgcgggga tcctctagag tcgagagatc tacgggtggc 3660
atccctgtga cccctcccca gtgcctctcc tggccctgga agttgccact ccagtgccca
3720 ccagccttgt cctaataaaa ttaagttgca tcattttgtc tgactaggtg
tccttctata 3780 atattatggg gtggaggggg gtggtatgga gcaaggggca
agttgggaag acaacctgta 3840 gggcctgcgg ggtctattgg gaaccaagct
ggagtgcagt ggcacaatct tggctcactg 3900 caatctccgc ctcctgggtt
caagcgattc tcctgcctca gcctcccgag ttgttgggat 3960 tccaggcatg
catgaccagg ctcagctaat ttttgttttt ttggtagaga cggggtttca 4020
ccatattggc caggctggtc tccaactcct aatctcaggt gatctaccca ccttggcctc
4080 ccaaattgct gggattacag gcgtgaacca ctgctccctt ccctgtcctt
ctgattttgt 4140 aggtaaccac gtgcggaccg agcggccgca ggaaccccta
gtgatggagt tggccactcc 4200 ctctctgcgc gctcgctcgc tcactgaggc
cgggcgacca aaggtcgccc gacgcccggg 4260 ctttgcccgg gcggcctcag
tgagcgagcg agcgcgcagc tgcctgcagg 4310 <210> SEQ ID NO 39
<211> LENGTH: 3602 <212> TYPE: DNA <213>
ORGANISM: Artificial Sequence <220> FEATURE: <223>
OTHER INFORMATION: 5'-
ITR-CAG-ChR2-{MLPH-Motif}-WPRE-bGHpolyA-ITR-3' <400>
SEQUENCE: 39 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag
cccgggcgtc 60 gggcgacctt tggtcgcccg gccgcagtga gcgagcgagc
gcgcagagag ggagtggcca 120 actccatcac taggggttcc tgcggccgca
cgcgtgatat cctagttatt aatagtaatc 180 aattacgggg tcattagttc
atagcccata tatggagttc cgcgttacat aacttacggt 240 aaatggcccg
cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta 300
tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
360 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
cccctattga 420 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
tacatgacct tatgggactt 480 tcctacttgg cagtacatct acgtattagt
catcgctatt accatgcatg gtcgaggtga 540 gccccacgtt ctgcttcact
ctccccatct cccccccctc cccaccccca attttgtatt 600 tatttatttt
ttaattattt tgtgcagcga tgggggcggg gggggggggg gggcgcgcgc 660
caggcggggc ggggcggggc gaggggcggg gcggggcgag gcggagaggt gcggcggcag
720 ccaatcagag cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg
cggcggcggc 780 cctataaaaa gcgaagcgcg cggcgggcgg gagtcgctgc
gacgctgcct tcgccccgtg 840 ccccgctccg ccgccgcctc gcgccgcccg
ccccggctct gactgaccgc gttactccca 900 caggtgagcg ggcgggacgg
cccttctcct ccgggctgta attagcgctt ggtttaatga 960 cggcttgttt
cttttctgtg gctgcgtgaa agccttgagg ggctccggga gggccctttg 1020
tgcgggggga gcggctcggg gctgtccgcg gggggacggc tgccttcggg ggggacgggg
1080 cagggcgggg ttcggcttct ggcgtgtgac cggcggctct agcagcctct
gctaaccatg 1140 ttcatgcctt cttctttttc ctacagctcc tgggcaacgt
gctggttatt gtgctgtctc 1200 atcattttgg caaagaatta agcttgagct
cgcgatccgc agccatggat tatggaggcg 1260 ccctgagtgc cgttgggcgc
gagctgctat ttgtaacgaa cccagtagtc gtcaatggct 1320 ctgtacttgt
gcctgaggac cagtgttact gcgcgggctg gattgagtcg cgtggcacaa 1380
acggtgccca aacggcgtcg aacgtgctgc aatggcttgc tgctggcttc tccatcctac
1440 tgcttatgtt ttacgcctac caaacatgga agtcaacctg cggctgggag
gagatctatg 1500 tgtgcgctat cgagatggtc aaggtgattc ttgagttctt
cttcgagttt aagaacccgt 1560 ccatgctgta tctagccaca ggccaccgcg
tccagtggtt gcgttacgcc gagtggcttc 1620 tcacctgccc ggtcattctc
attcacctgt caaacctgac gggcttgtcc aacgactaca 1680 gcaggcgcac
tatgggtctg cttgtgtctg atattggcac aattgtgtgg ggcgccactt 1740
ccgctatggc caccggatac gtcaaggtca tcttcttctg cctgggtctg tgttatggtg
1800 ctaacacgtt ctttcacgct gccaaggcct acatcgaggg ttaccatacc
gtgccgaagg 1860 gccggtgtcg ccaggtggtg actggcatgg cttggctctt
cttcgtatca tggggtatgt 1920 tccccatcct gttcatcctc ggccccgagg
gcttcggcgt cctgagcgtg tacggctcca 1980 ccgtcggcca caccatcatt
gacctgatgt cgaagaactg ctggggtctg ctcggccact 2040 acctgcgcgt
gctgatccac gagcatatcc tcatccacgg cgacattcgc aagaccacca 2100
aattgaacat tggtggcact gagattgagg tcgagacgct ggtggaggac gaggccgagg
2160 ctggcgcggt caacaagggc accggcaagg aattcggagg cggaggtgga
gctagcaggg 2220 accagcctct gaacagcaaa aagaaaaaga ggctcctgag
cttcagggac gtggacttcg 2280 aggaggacag cgactaactc gagtctagac
gtggtaccga taatcaacct ctggattaca 2340 aaatttgtga aagattgact
ggtattctta actatgttgc tccttttacg ctatgtggat 2400 acgctgcttt
aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct 2460
ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
2520 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
attgccacca 2580 cctgtcagct cctttccggg actttcgctt tccccctccc
tattgccacg gcggaactca 2640 tcgccgcctg ccttgcccgc tgctggacag
gggctcggct gttgggcact gacaattccg 2700 tggtgttgtc ggggaagctg
acgtcctttc catggctgct cgcctgtgtt gccacctgga 2760 ttctgcgcgg
gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt 2820
cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga
2880 gtcggatctc cctttgggcc gcctccccgc ctgatgcggg gatcctctag
agtcgagaga 2940 tctacgggtg gcatccctgt gacccctccc cagtgcctct
cctggccctg gaagttgcca 3000 ctccagtgcc caccagcctt gtcctaataa
aattaagttg catcattttg tctgactagg 3060 tgtccttcta taatattatg
gggtggaggg gggtggtatg gagcaagggg caagttggga 3120 agacaacctg
tagggcctgc ggggtctatt gggaaccaag ctggagtgca gtggcacaat 3180
cttggctcac tgcaatctcc gcctcctggg ttcaagcgat tctcctgcct cagcctcccg
3240 agttgttggg attccaggca tgcatgacca ggctcagcta atttttgttt
ttttggtaga 3300 gacggggttt caccatattg gccaggctgg tctccaactc
ctaatctcag gtgatctacc 3360 caccttggcc tcccaaattg ctgggattac
aggcgtgaac cactgctccc ttccctgtcc 3420 ttctgatttt gtaggtaacc
acgtgcggac cgagcggccg caggaacccc tagtgatgga 3480 gttggccact
ccctctctgc gcgctcgctc gctcactgag gccgggcgac caaaggtcgc 3540
ccgacgcccg ggctttgccc gggcggcctc agtgagcgag cgagcgcgca gctgcctgca
3600 gg 3602 <210> SEQ ID NO 40 <211> LENGTH: 4361
<212> TYPE: DNA <213> ORGANISM: Artificial Sequence
<220> FEATURE: <223> OTHER INFORMATION: 5'-
ITR-CAG-HaloR-GFP-{Kv2 .lMotif}- WPRE-bGHpolyA-ITR-3' <400>
SEQUENCE: 40 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag
cccgggcgtc 60 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc
gcgcagagag ggagtggcca 120 actccatcac taggggttcc tgcggccgca
cgcgtgatat cctagttatt aatagtaatc 180 aattacgggg tcattagttc
atagcccata tatggagttc cgcgttacat aacttacggt 240 aaatggcccg
cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta 300
tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
360 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
cccctattga 420 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
tacatgacct tatgggactt 480 tcctacttgg cagtacatct acgtattagt
catcgctatt accatgcatg gtcgaggtga 540 gccccacgtt ctgcttcact
ctccccatct cccccccctc cccaccccca attttgtatt 600 tatttatttt
ttaattattt tgtgcagcga tgggggcggg gggggggggg gggcgcgcgc 660
caggcggggc ggggcggggc gaggggcggg gcggggcgag gcggagaggt gcggcggcag
720 ccaatcagag cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg
cggcggcggc 780 cctataaaaa gcgaagcgcg cggcgggcgg gagtcgctgc
gacgctgcct tcgccccgtg 840 ccccgctccg ccgccgcctc gcgccgcccg
ccccggctct gactgaccgc gttactccca 900 caggtgagcg ggcgggacgg
cccttctcct ccgggctgta attagcgctt ggtttaatga 960 cggcttgttt
cttttctgtg gctgcgtgaa agccttgagg ggctccggga gggccctttg 1020
tgcgggggga gcggctcggg gctgtccgcg gggggacggc tgccttcggg ggggacgggg
1080 cagggcgggg ttcggcttct ggcgtgtgac cggcggctct agcagcctct
gctaaccatg 1140 ttcatgcctt cttctttttc ctacagctcc tgggcaacgt
gctggttatt gtgctgtctc 1200 atcattttgg caaagaatta agcttgagct
cgcgatccgc agccatgact gagacattgc 1260 caccggtaac ggaatcggct
gttgcgctac aggcggaggt gacccagagg gagctgttcg 1320 agttcgttct
caacgacccc ctcctcgcca gttcgctgta tattaatatc gcactggcag 1380
ggctgtcgat actgcttttc gtgttcatga cgcgcggact cgacgaccca cgggcgaaac
1440 tcatcgccgt ttcgacgatt ttggtgccgg tggtctctat cgcgagctac
accggccttg 1500 catcggggct caccatcagc gtcctcgaga tgccagccgg
ccacttcgcc gaggggtcct 1560 cggtgatgct cggcggcgaa gaggtagacg
gcgtcgtgac gatgtggggc cgctatctga 1620 cgtgggccct ttcgacaccg
atgatactgc tggcgcttgg gctgcttgct ggctctaacg 1680 ccacgaagct
ctttaccgcc atcaccttcg acatcgcgat gtgtgtcacc ggcctcgcag 1740
ccgcgctgac gacctcttcg cacctgatgc ggtggttctg gtacgccatc agttgtgcgt
1800 gtttcctcgt cgtcctctac atcctgctcg tcgagtgggc acaggacgcc
aaggctgccg 1860 gtactgcgga tatgttcaat acgctgaagc tgctgaccgt
tgtcatgtgg ctcggctacc 1920 ccatcgtgtg ggcactcggc gttgagggca
tcgccgttct tccggtcgga gtcacgtcgt 1980 ggggatacag cttcctcgac
atcgtcgcga agtacatctt cgcgttcctg ctgctcaact 2040 acctcacgtc
gaacgagagc gtcgtctccg gctcgatact cgacgtgccg tccgcgtcgg 2100
gcactcccgc tgacgacgaa ttcggaggcg gaggtggagc tagcaaagga gaagaactct
2160 tcactggagt tggagttgtc ccaattcttg ttgaattaga tggtgatgtt
aacggccaca 2220 agttctctgt cagtggagag ggtgaaggtg atgcaacata
cggaaaactt accctgaagt 2280 tcatctgcac tactggcaaa ctgcctgttc
catggccaac actagtcact actctgtgct 2340 atggtgttca atgcttttca
agatacccgg atcatatgaa acggcatgac tttttcaaga 2400 gtgccatgcc
cgaaggttat gtacaggaaa ggaccatctt cttcaaagat gacggcaact 2460
acaagacacg tgctgaagtc aagtttgaag gtgataccct tgttaataga atcgagttaa
2520 aaggtattga cttcaaggaa gatggcaaca ttctgggaca caaattggaa
tacaactata 2580 actcacacaa tgtatacatc atggcagaca aacaaaagaa
tggaatcaaa gtgaacttca 2640 agacccgcca caacattgaa gatggaagcg
ttcaactagc agaccattat caacaaaata 2700 ctccaattgg cgatggccct
gtccttttac cagacaacca ttacctgtcc acacaatctg 2760 ccctttcgaa
agatcccaac gaaaagagag accacatggt ccttcttgag tttgtaacag 2820
ctgctgggat tacacatggc atggatgaac tgtacaacca gtctcagccc atcctgaaca
2880 ctaaggagat ggcccctcag agtaaacccc ctgaggaact ggaaatgagc
tccatgccat 2940 ctccagtggc tcctctgcca gctaggaccg agggcgtgat
tgacatgaga agcatgtcta 3000 gtatcgatag cttcatttcc tgcgccaccg
acttccccga agctacaagg ttttaactcg 3060 agtctagacg tggtaccgat
aatcaacctc tggattacaa aatttgtgaa agattgactg 3120 gtattcttaa
ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt 3180
atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
3240 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
tgcactgtgt 3300 ttgctgacgc aacccccact ggttggggca ttgccaccac
ctgtcagctc ctttccggga 3360 ctttcgcttt ccccctccct attgccacgg
cggaactcat cgccgcctgc cttgcccgct 3420 gctggacagg ggctcggctg
ttgggcactg acaattccgt ggtgttgtcg gggaagctga 3480 cgtcctttcc
atggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct 3540
gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
3600 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
ctttgggccg 3660 cctccccgcc tgatgcgggg atcctctaga gtcgagagat
ctacgggtgg catccctgtg 3720 acccctcccc agtgcctctc ctggccctgg
aagttgccac tccagtgccc accagccttg 3780 tcctaataaa attaagttgc
atcattttgt ctgactaggt gtccttctat aatattatgg 3840 ggtggagggg
ggtggtatgg agcaaggggc aagttgggaa gacaacctgt agggcctgcg 3900
gggtctattg ggaaccaagc tggagtgcag tggcacaatc ttggctcact gcaatctccg
3960 cctcctgggt tcaagcgatt ctcctgcctc agcctcccga gttgttggga
ttccaggcat 4020 gcatgaccag gctcagctaa tttttgtttt tttggtagag
acggggtttc accatattgg 4080 ccaggctggt ctccaactcc taatctcagg
tgatctaccc accttggcct cccaaattgc 4140 tgggattaca ggcgtgaacc
actgctccct tccctgtcct tctgattttg taggtaacca 4200 cgtgcggacc
gagcggccgc aggaacccct agtgatggag ttggccactc cctctctgcg 4260
cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg
4320 ggcggcctca gtgagcgagc gagcgcgcag ctgcctgcag g 4361 <210>
SEQ ID NO 41 <211> LENGTH: 3647 <212> TYPE: DNA
<213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: 5' -
ITR-CAG-HaloR-{Kv2.1Motif}-WPRE-bGHpolyA-ITR- 3' <400>
SEQUENCE: 41 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag
cccgggcgtc 60 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc
gcgcagagag ggagtggcca 120 actccatcac taggggttcc tgcggccgca
cgcgtgatat cctagttatt aatagtaatc 180 aattacgggg tcattagttc
atagcccata tatggagttc cgcgttacat aacttacggt 240 aaatggcccg
cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta 300
tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
360 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
cccctattga 420 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
tacatgacct tatgggactt 480 tcctacttgg cagtacatct acgtattagt
catcgctatt accatgcatg gtcgaggtga 540 gccccacgtt ctgcttcact
ctccccatct cccccccctc cccaccccca attttgtatt 600 tatttatttt
ttaattattt tgtgcagcga tgggggcggg gggggggggg gggcgcgcgc 660
caggcggggc ggggcggggc gaggggcggg gcggggcgag gcggagaggt gcggcggcag
720 ccaatcagag cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg
cggcggcggc 780 cctataaaaa gcgaagcgcg cggcgggcgg gagtcgctgc
gacgctgcct tcgccccgtg 840 ccccgctccg ccgccgcctc gcgccgcccg
ccccggctct gactgaccgc gttactccca 900 caggtgagcg ggcgggacgg
cccttctcct ccgggctgta attagcgctt ggtttaatga 960 cggcttgttt
cttttctgtg gctgcgtgaa agccttgagg ggctccggga gggccctttg 1020
tgcgggggga gcggctcggg gctgtccgcg gggggacggc tgccttcggg ggggacgggg
1080 cagggcgggg ttcggcttct ggcgtgtgac cggcggctct agcagcctct
gctaaccatg 1140 ttcatgcctt cttctttttc ctacagctcc tgggcaacgt
gctggttatt gtgctgtctc 1200 atcattttgg caaagaatta agcttgagct
cgcgatccgc agccatgact gagacattgc 1260 caccggtaac ggaatcggct
gttgcgctac aggcggaggt gacccagagg gagctgttcg 1320 agttcgttct
caacgacccc ctcctcgcca gttcgctgta tattaatatc gcactggcag 1380
ggctgtcgat actgcttttc gtgttcatga cgcgcggact cgacgaccca cgggcgaaac
1440 tcatcgccgt ttcgacgatt ttggtgccgg tggtctctat cgcgagctac
accggccttg 1500 catcggggct caccatcagc gtcctcgaga tgccagccgg
ccacttcgcc gaggggtcct 1560 cggtgatgct cggcggcgaa gaggtagacg
gcgtcgtgac gatgtggggc cgctatctga 1620 cgtgggccct ttcgacaccg
atgatactgc tggcgcttgg gctgcttgct ggctctaacg 1680 ccacgaagct
ctttaccgcc atcaccttcg acatcgcgat gtgtgtcacc ggcctcgcag 1740
ccgcgctgac gacctcttcg cacctgatgc ggtggttctg gtacgccatc agttgtgcgt
1800 gtttcctcgt cgtcctctac atcctgctcg tcgagtgggc acaggacgcc
aaggctgccg 1860 gtactgcgga tatgttcaat acgctgaagc tgctgaccgt
tgtcatgtgg ctcggctacc 1920 ccatcgtgtg ggcactcggc gttgagggca
tcgccgttct tccggtcgga gtcacgtcgt 1980 ggggatacag cttcctcgac
atcgtcgcga agtacatctt cgcgttcctg ctgctcaact 2040 acctcacgtc
gaacgagagc gtcgtctccg gctcgatact cgacgtgccg tccgcgtcgg 2100
gcactcccgc tgacgacgaa ttcggaggcg gaggtggagc tagccagtct cagcccatcc
2160 tgaacactaa ggagatggcc cctcagagta aaccccctga ggaactggaa
atgagctcca 2220 tgccatctcc agtggctcct ctgccagcta ggaccgaggg
cgtgattgac atgagaagca 2280 tgtctagtat cgatagcttc atttcctgcg
ccaccgactt ccccgaagct acaaggtttt 2340 aactcgagtc tagacgtggt
accgataatc aacctctgga ttacaaaatt tgtgaaagat 2400 tgactggtat
tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc 2460
ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
2520 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
gtggtgtgca 2580 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
caccacctgt cagctccttt 2640 ccgggacttt cgctttcccc ctccctattg
ccacggcgga actcatcgcc gcctgccttg 2700 cccgctgctg gacaggggct
cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga 2760 agctgacgtc
ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt 2820
ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
2880 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
atctcccttt 2940 gggccgcctc cccgcctgat gcggggatcc tctagagtcg
agagatctac gggtggcatc 3000 cctgtgaccc ctccccagtg cctctcctgg
ccctggaagt tgccactcca gtgcccacca 3060 gccttgtcct aataaaatta
agttgcatca ttttgtctga ctaggtgtcc ttctataata 3120 ttatggggtg
gaggggggtg gtatggagca aggggcaagt tgggaagaca acctgtaggg 3180
cctgcggggt ctattgggaa ccaagctgga gtgcagtggc acaatcttgg ctcactgcaa
3240 tctccgcctc ctgggttcaa gcgattctcc tgcctcagcc tcccgagttg
ttgggattcc 3300 aggcatgcat gaccaggctc agctaatttt tgtttttttg
gtagagacgg ggtttcacca 3360 tattggccag gctggtctcc aactcctaat
ctcaggtgat ctacccacct tggcctccca 3420 aattgctggg attacaggcg
tgaaccactg ctcccttccc tgtccttctg attttgtagg 3480 taaccacgtg
cggaccgagc ggccgcagga acccctagtg atggagttgg ccactccctc 3540
tctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac gcccgggctt
3600 tgcccgggcg gcctcagtga gcgagcgagc gcgcagctgc ctgcagg 3647
<210> SEQ ID NO 42 <211> LENGTH: 4247 <212> TYPE:
DNA <213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: 5' - ITR-CAG-HaloR-GFP-{Nav1.6
Motif}-WPRE-bGHpolyA-ITR- 3' <400> SEQUENCE: 42 cctgcaggca
gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc 60
gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
120 actccatcac taggggttcc tgcggccgca cgcgtgatat cctagttatt
aatagtaatc 180 aattacgggg tcattagttc atagcccata tatggagttc
cgcgttacat aacttacggt 240 aaatggcccg cctggctgac cgcccaacga
cccccgccca ttgacgtcaa taatgacgta 300 tgttcccata gtaacgccaa
tagggacttt ccattgacgt caatgggtgg agtatttacg 360 gtaaactgcc
cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga 420
cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
480 tcctacttgg cagtacatct acgtattagt catcgctatt accatgcatg
gtcgaggtga 540 gccccacgtt ctgcttcact ctccccatct cccccccctc
cccaccccca attttgtatt 600 tatttatttt ttaattattt tgtgcagcga
tgggggcggg gggggggggg gggcgcgcgc 660 caggcggggc ggggcggggc
gaggggcggg gcggggcgag gcggagaggt gcggcggcag 720 ccaatcagag
cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg cggcggcggc 780
cctataaaaa gcgaagcgcg cggcgggcgg gagtcgctgc gacgctgcct tcgccccgtg
840 ccccgctccg ccgccgcctc gcgccgcccg ccccggctct gactgaccgc
gttactccca 900 caggtgagcg ggcgggacgg cccttctcct ccgggctgta
attagcgctt ggtttaatga 960 cggcttgttt cttttctgtg gctgcgtgaa
agccttgagg ggctccggga gggccctttg 1020 tgcgggggga gcggctcggg
gctgtccgcg gggggacggc tgccttcggg ggggacgggg 1080 cagggcgggg
ttcggcttct ggcgtgtgac cggcggctct agcagcctct gctaaccatg 1140
ttcatgcctt cttctttttc ctacagctcc tgggcaacgt gctggttatt gtgctgtctc
1200 atcattttgg caaagaatta agcttgagct cgcgatccgc agccatgact
gagacattgc 1260 caccggtaac ggaatcggct gttgcgctac aggcggaggt
gacccagagg gagctgttcg 1320 agttcgttct caacgacccc ctcctcgcca
gttcgctgta tattaatatc gcactggcag 1380 ggctgtcgat actgcttttc
gtgttcatga cgcgcggact cgacgaccca cgggcgaaac 1440 tcatcgccgt
ttcgacgatt ttggtgccgg tggtctctat cgcgagctac accggccttg 1500
catcggggct caccatcagc gtcctcgaga tgccagccgg ccacttcgcc gaggggtcct
1560 cggtgatgct cggcggcgaa gaggtagacg gcgtcgtgac gatgtggggc
cgctatctga 1620 cgtgggccct ttcgacaccg atgatactgc tggcgcttgg
gctgcttgct ggctctaacg 1680 ccacgaagct ctttaccgcc atcaccttcg
acatcgcgat gtgtgtcacc ggcctcgcag 1740 ccgcgctgac gacctcttcg
cacctgatgc ggtggttctg gtacgccatc agttgtgcgt 1800 gtttcctcgt
cgtcctctac atcctgctcg tcgagtgggc acaggacgcc aaggctgccg 1860
gtactgcgga tatgttcaat acgctgaagc tgctgaccgt tgtcatgtgg ctcggctacc
1920 ccatcgtgtg ggcactcggc gttgagggca tcgccgttct tccggtcgga
gtcacgtcgt 1980 ggggatacag cttcctcgac atcgtcgcga agtacatctt
cgcgttcctg ctgctcaact 2040 acctcacgtc gaacgagagc gtcgtctccg
gctcgatact cgacgtgccg tccgcgtcgg 2100 gcactcccgc tgacgacgaa
ttcggaggcg gaggtggagc tagcaaagga gaagaactct 2160 tcactggagt
tggagttgtc ccaattcttg ttgaattaga tggtgatgtt aacggccaca 2220
agttctctgt cagtggagag ggtgaaggtg atgcaacata cggaaaactt accctgaagt
2280 tcatctgcac tactggcaaa ctgcctgttc catggccaac actagtcact
actctgtgct 2340 atggtgttca atgcttttca agatacccgg atcatatgaa
acggcatgac tttttcaaga 2400 gtgccatgcc cgaaggttat gtacaggaaa
ggaccatctt cttcaaagat gacggcaact 2460 acaagacacg tgctgaagtc
aagtttgaag gtgataccct tgttaataga atcgagttaa 2520 aaggtattga
cttcaaggaa gatggcaaca ttctgggaca caaattggaa tacaactata 2580
actcacacaa tgtatacatc atggcagaca aacaaaagaa tggaatcaaa gtgaacttca
2640 agacccgcca caacattgaa gatggaagcg ttcaactagc agaccattat
caacaaaata 2700 ctccaattgg cgatggccct gtccttttac cagacaacca
ttacctgtcc acacaatctg 2760 ccctttcgaa agatcccaac gaaaagagag
accacatggt ccttcttgag tttgtaacag 2820 ctgctgggat tacacatggc
atggatgaac tgtacaacac cgtgagggtg cccatcgccg 2880 tgggcgagag
cgacttcgag aacctgaaca ccgaggacgt gagcagcgag agcgacccct 2940
aactcgagtc tagacgtggt accgataatc aacctctgga ttacaaaatt tgtgaaagat
3000 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
gctttaatgc 3060 ctttgtatca tgctattgct tcccgtatgg ctttcatttt
ctcctccttg tataaatcct 3120 ggttgctgtc tctttatgag gagttgtggc
ccgttgtcag gcaacgtggc gtggtgtgca 3180 ctgtgtttgc tgacgcaacc
cccactggtt ggggcattgc caccacctgt cagctccttt 3240 ccgggacttt
cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg 3300
cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
3360 agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg
cgcgggacgt 3420 ccttctgcta cgtcccttcg gccctcaatc cagcggacct
tccttcccgc ggcctgctgc 3480 cggctctgcg gcctcttccg cgtcttcgcc
ttcgccctca gacgagtcgg atctcccttt 3540 gggccgcctc cccgcctgat
gcggggatcc tctagagtcg agagatctac gggtggcatc 3600 cctgtgaccc
ctccccagtg cctctcctgg ccctggaagt tgccactcca gtgcccacca 3660
gccttgtcct aataaaatta agttgcatca ttttgtctga ctaggtgtcc ttctataata
3720 ttatggggtg gaggggggtg gtatggagca aggggcaagt tgggaagaca
acctgtaggg 3780 cctgcggggt ctattgggaa ccaagctgga gtgcagtggc
acaatcttgg ctcactgcaa 3840 tctccgcctc ctgggttcaa gcgattctcc
tgcctcagcc tcccgagttg ttgggattcc 3900 aggcatgcat gaccaggctc
agctaatttt tgtttttttg gtagagacgg ggtttcacca 3960 tattggccag
gctggtctcc aactcctaat ctcaggtgat ctacccacct tggcctccca 4020
aattgctggg attacaggcg tgaaccactg ctcccttccc tgtccttctg attttgtagg
4080 taaccacgtg cggaccgagc ggccgcagga acccctagtg atggagttgg
ccactccctc 4140 tctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag
gtcgcccgac gcccgggctt 4200 tgcccgggcg gccgcagtga gcgagcgagc
gcgcagctgc ctgcagg 4247 <210> SEQ ID NO 43 <211>
LENGTH: 3533 <212> TYPE: DNA <213> ORGANISM: Artificial
Sequence <220> FEATURE: <223> OTHER INFORMATION: 5'-
ITR-CAG-HaloR-{Nav1.6 Motif}-WPRE-bGHpolyA-ITR- 3' <400>
SEQUENCE: 43 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag
cccgggcgtc 60 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc
gcgcagagag ggagtggcca 120 actccatcac taggggttcc tgcggccgca
cgcgtgatat cctagttatt aatagtaatc 180 aattacgggg tcattagttc
atagcccata tatggagttc cgcgttacat aacttacggt 240 aaatggcccg
cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta 300
tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
360 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
cccctattga 420 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
tacatgacct tatgggactt 480 tcctacttgg cagtacatct acgtattagt
catcgctatt accatgcatg gtcgaggtga 540 gccccacgtt ctgcttcact
ctccccatct cccccccctc cccaccccca attttgtatt 600 tatttatttt
ttaattattt tgtgcagcga tgggggcggg gggggggggg gggcgcgcgc 660
caggcggggc ggggcggggc gaggggcggg gcggggcgag gcggagaggt gcggcggcag
720 ccaatcagag cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg
cggcggcggc 780 cctataaaaa gcgaagcgcg cggcgggcgg gagtcgctgc
gacgctgcct tcgccccgtg 840 ccccgctccg ccgccgcctc gcgccgcccg
ccccggctct gactgaccgc gttactccca 900 caggtgagcg ggcgggacgg
cccttctcct ccgggctgta attagcgctt ggtttaatga 960 cggcttgttt
cttttctgtg gctgcgtgaa agccttgagg ggctccggga gggccctttg 1020
tgcgggggga gcggctcggg gctgtccgcg gggggacggc tgccttcggg ggggacgggg
1080 cagggcgggg ttcggcttct ggcgtgtgac cggcggctct agcagcctct
gctaaccatg 1140 ttcatgcctt cttctttttc ctacagctcc tgggcaacgt
gctggttatt gtgctgtctc 1200 atcattttgg caaagaatta agcttgagct
cgcgatccgc agccatgact gagacattgc 1260 caccggtaac ggaatcggct
gttgcgctac aggcggaggt gacccagagg gagctgttcg 1320 agttcgttct
caacgacccc ctcctcgcca gttcgctgta tattaatatc gcactggcag 1380
ggctgtcgat actgcttttc gtgttcatga cgcgcggact cgacgaccca cgggcgaaac
1440 tcatcgccgt ttcgacgatt ttggtgccgg tggtctctat cgcgagctac
accggccttg 1500 catcggggct caccatcagc gtcctcgaga tgccagccgg
ccacttcgcc gaggggtcct 1560 cggtgatgct cggcggcgaa gaggtagacg
gcgtcgtgac gatgtggggc cgctatctga 1620 cgtgggccct ttcgacaccg
atgatactgc tggcgcttgg gctgcttgct ggctctaacg 1680 ccacgaagct
ctttaccgcc atcaccttcg acatcgcgat gtgtgtcacc ggcctcgcag 1740
ccgcgctgac gacctcttcg cacctgatgc ggtggttctg gtacgccatc agttgtgcgt
1800 gtttcctcgt cgtcctctac atcctgctcg tcgagtgggc acaggacgcc
aaggctgccg 1860 gtactgcgga tatgttcaat acgctgaagc tgctgaccgt
tgtcatgtgg ctcggctacc 1920 ccatcgtgtg ggcactcggc gttgagggca
tcgccgttct tccggtcgga gtcacgtcgt 1980 ggggatacag cttcctcgac
atcgtcgcga agtacatctt cgcgttcctg ctgctcaact 2040 acctcacgtc
gaacgagagc gtcgtctccg gctcgatact cgacgtgccg tccgcgtcgg 2100
gcactcccgc tgacgacgaa ttcggaggcg gaggtggagc tagcaccgtg agggtgccca
2160 tcgccgtggg cgagagcgac ttcgagaacc tgaacaccga ggacgtgagc
agcgagagcg 2220 acccctaact cgagtctaga cgtggtaccg ataatcaacc
tctggattac aaaatttgtg 2280 aaagattgac tggtattctt aactatgttg
ctccttttac gctatgtgga tacgctgctt 2340 taatgccttt gtatcatgct
attgcttccc gtatggcttt cattttctcc tccttgtata 2400 aatcctggtt
gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg 2460
tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
2520 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
atcgccgcct 2580 gccttgcccg ctgctggaca ggggctcggc tgttgggcac
tgacaattcc gtggtgttgt 2640 cggggaagct gacgtccttt ccatggctgc
tcgcctgtgt tgccacctgg attctgcgcg 2700 ggacgtcctt ctgctacgtc
ccttcggccc tcaatccagc ggaccttcct tcccgcggcc 2760 tgctgccggc
tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct 2820
ccctttgggc cgcctccccg cctgatgcgg ggatcctcta gagtcgagag atctacgggt
2880 ggcatccctg tgacccctcc ccagtgcctc tcctggccct ggaagttgcc
actccagtgc 2940 ccaccagcct tgtcctaata aaattaagtt gcatcatttt
gtctgactag gtgtccttct 3000 ataatattat ggggtggagg ggggtggtat
ggagcaaggg gcaagttggg aagacaacct 3060 gtagggcctg cggggtctat
tgggaaccaa gctggagtgc agtggcacaa tcttggctca 3120 ctgcaatctc
cgcctcctgg gttcaagcga ttctcctgcc tcagcctccc gagttgttgg 3180
gattccaggc atgcatgacc aggctcagct aatttttgtt tttttggtag agacggggtt
3240 tcaccatatt ggccaggctg gtctccaact cctaatctca ggtgatctac
ccaccttggc 3300 ctcccaaatt gctgggatta caggcgtgaa ccactgctcc
cttccctgtc cttctgattt 3360 tgtaggtaac cacgtgcgga ccgagcggcc
gcaggaaccc ctagtgatgg agttggccac 3420 tccctctctg cgcgctcgct
cgctcactga ggccgggcga ccaaaggtcg cccgacgccc 3480 gggctttgcc
cgggcggcct cagtgagcga gcgagcgcgc agctgcctgc agg 3533 <210>
SEQ ID NO 44 <211> LENGTH: 4262 <212> TYPE: DNA
<213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: 5' -ITR-CAG-HaloR-GFP- {NLG-1
Motif}-WPRE-bGHpolyA-ITR- 3' <400> SEQUENCE: 44 cctgcaggca
gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc 60
gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
120 actccatcac taggggttcc tgcggccgca cgcgtgatat cctagttatt
aatagtaatc 180 aattacgggg tcattagttc atagcccata tatggagttc
cgcgttacat aacttacggt 240 aaatggcccg cctggctgac cgcccaacga
cccccgccca ttgacgtcaa taatgacgta 300 tgttcccata gtaacgccaa
tagggacttt ccattgacgt caatgggtgg agtatttacg 360 gtaaactgcc
cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga 420
cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
480 tcctacttgg cagtacatct acgtattagt catcgctatt accatgcatg
gtcgaggtga 540 gccccacgtt ctgcttcact ctccccatct cccccccctc
cccaccccca attttgtatt 600 tatttatttt ttaattattt tgtgcagcga
tgggggcggg gggggggggg gggcgcgcgc 660 caggcggggc ggggcggggc
gaggggcggg gcggggcgag gcggagaggt gcggcggcag 720 ccaatcagag
cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg cggcggcggc 780
cctataaaaa gcgaagcgcg cggcgggcgg gagtcgctgc gacgctgcct tcgccccgtg
840 ccccgctccg ccgccgcctc gcgccgcccg ccccggctct gactgaccgc
gttactccca 900 caggtgagcg ggcgggacgg cccttctcct ccgggctgta
attagcgctt ggtttaatga 960 cggcttgttt cttttctgtg gctgcgtgaa
agccttgagg ggctccggga gggccctttg 1020 tgcgggggga gcggctcggg
gctgtccgcg gggggacggc tgccttcggg ggggacgggg 1080 cagggcgggg
ttcggcttct ggcgtgtgac cggcggctct agcagcctct gctaaccatg 1140
ttcatgcctt cttctttttc ctacagctcc tgggcaacgt gctggttatt gtgctgtctc
1200 atcattttgg caaagaatta agcttgagct cgcgatccgc agccatgact
gagacattgc 1260 caccggtaac ggaatcggct gttgcgctac aggcggaggt
gacccagagg gagctgttcg 1320 agttcgttct caacgacccc ctcctcgcca
gttcgctgta tattaatatc gcactggcag 1380 ggctgtcgat actgcttttc
gtgttcatga cgcgcggact cgacgaccca cgggcgaaac 1440 tcatcgccgt
ttcgacgatt ttggtgccgg tggtctctat cgcgagctac accggccttg 1500
catcggggct caccatcagc gtcctcgaga tgccagccgg ccacttcgcc gaggggtcct
1560 cggtgatgct cggcggcgaa gaggtagacg gcgtcgtgac gatgtggggc
cgctatctga 1620 cgtgggccct ttcgacaccg atgatactgc tggcgcttgg
gctgcttgct ggctctaacg 1680 ccacgaagct ctttaccgcc atcaccttcg
acatcgcgat gtgtgtcacc ggcctcgcag 1740 ccgcgctgac gacctcttcg
cacctgatgc ggtggttctg gtacgccatc agttgtgcgt 1800 gtttcctcgt
cgtcctctac atcctgctcg tcgagtgggc acaggacgcc aaggctgccg 1860
gtactgcgga tatgttcaat acgctgaagc tgctgaccgt tgtcatgtgg ctcggctacc
1920 ccatcgtgtg ggcactcggc gttgagggca tcgccgttct tccggtcgga
gtcacgtcgt 1980 ggggatacag cttcctcgac atcgtcgcga agtacatctt
cgcgttcctg ctgctcaact 2040 acctcacgtc gaacgagagc gtcgtctccg
gctcgatact cgacgtgccg tccgcgtcgg 2100 gcactcccgc tgacgacgaa
ttcggaggcg gaggtggagc tagcaaagga gaagaactct 2160 tcactggagt
tggagttgtc ccaattcttg ttgaattaga tggtgatgtt aacggccaca 2220
agttctctgt cagtggagag ggtgaaggtg atgcaacata cggaaaactt accctgaagt
2280 tcatctgcac tactggcaaa ctgcctgttc catggccaac actagtcact
actctgtgct 2340 atggtgttca atgcttttca agatacccgg atcatatgaa
acggcatgac tttttcaaga 2400 gtgccatgcc cgaaggttat gtacaggaaa
ggaccatctt cttcaaagat gacggcaact 2460 acaagacacg tgctgaagtc
aagtttgaag gtgataccct tgttaataga atcgagttaa 2520 aaggtattga
cttcaaggaa gatggcaaca ttctgggaca caaattggaa tacaactata 2580
actcacacaa tgtatacatc atggcagaca aacaaaagaa tggaatcaaa gtgaacttca
2640 agacccgcca caacattgaa gatggaagcg ttcaactagc agaccattat
caacaaaata 2700 ctccaattgg cgatggccct gtccttttac cagacaacca
ttacctgtcc acacaatctg 2760 ccctttcgaa agatcccaac gaaaagagag
accacatggt ccttcttgag tttgtaacag 2820 ctgctgggat tacacatggc
atggatgaac tgtacaacgt ggttcttcgg accgcctgtc 2880 ccccagatta
cacactagct atgaggaggt cacctgatga tgttccctta atgacaccca 2940
acaccattac aatgtaactc gagtctagac gtggtaccga taatcaacct ctggattaca
3000 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
ctatgtggat 3060 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
tatggctttc attttctcct 3120 ccttgtataa atcctggttg ctgtctcttt
atgaggagtt gtggcccgtt gtcaggcaac 3180 gtggcgtggt gtgcactgtg
tttgctgacg caacccccac tggttggggc attgccacca 3240 cctgtcagct
cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca 3300
tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
3360 tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt
gccacctgga 3420 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
caatccagcg gaccttcctt 3480 cccgcggcct gctgccggct ctgcggcctc
ttccgcgtct tcgccttcgc cctcagacga 3540 gtcggatctc cctttgggcc
gcctccccgc ctgatgcggg gatcctctag agtcgagaga 3600 tctacgggtg
gcatccctgt gacccctccc cagtgcctct cctggccctg gaagttgcca 3660
ctccagtgcc caccagcctt gtcctaataa aattaagttg catcattttg tctgactagg
3720 tgtccttcta taatattatg gggtggaggg gggtggtatg gagcaagggg
caagttggga 3780 agacaacctg tagggcctgc ggggtctatt gggaaccaag
ctggagtgca gtggcacaat 3840 cttggctcac tgcaatctcc gcctcctggg
ttcaagcgat tctcctgcct cagcctcccg 3900 agttgttggg attccaggca
tgcatgacca ggctcagcta atttttgttt ttttggtaga 3960 gacggggttt
caccatattg gccaggctgg tctccaactc ctaatctcag gtgatctacc 4020
caccttggcc tcccaaattg ctgggattac aggcgtgaac cactgctccc ttccctgtcc
4080 ttctgatttt gtaggtaacc acgtgcggac cgagcggccg caggaacccc
tagtgatgga 4140 gttggccact ccctctctgc gcgctcgctc gctcactgag
gccgggcgac caaaggtcgc 4200 ccgacgcccg ggctttgccc gggcggcctc
agtgagcgag cgagcgcgca gctgcctgca 4260 gg 4262 <210> SEQ ID NO
45 <211> LENGTH: 3626 <212> TYPE: DNA <213>
ORGANISM: Artificial Sequence <220> FEATURE: <223>
OTHER INFORMATION: 5' -ITR-CAG-HaloR-{NLG-1
Motif}-WPRE-bGHpolyA-ITR- 3' <400> SEQUENCE: 45 cctgcaggca
gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc 60
gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
120 actccatcac taggggttcc tgcggccgca cgcgtgatat cctagttatt
aatagtaatc 180 aattacgggg tcattagttc atagcccata tatggagttc
cgcgttacat aacttacggt 240 aaatggcccg cctggctgac cgcccaacga
cccccgccca ttgacgtcaa taatgacgta 300 tgttcccata gtaacgccaa
tagggacttt ccattgacgt caatgggtgg agtatttacg 360 gtaaactgcc
cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga 420
cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
480 tcctacttgg cagtacatct acgtattagt catcgctatt accatgcatg
gtcgaggtga 540 gccccacgtt ctgcttcact ctccccatct cccccccctc
cccaccccca attttgtatt 600 tatttatttt ttaattattt tgtgcagcga
tgggggcggg gggggggggg gggcgcgcgc 660 caggcggggc ggggcggggc
gaggggcggg gcggggcgag gcggagaggt gcggcggcag 720 ccaatcagag
cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg cggcggcggc 780
cctataaaaa gcgaagcgcg cggcgggcgg gagtcgctgc gacgctgcct tcgccccgtg
840 ccccgctccg ccgccgcctc gcgccgcccg ccccggctct gactgaccgc
gttactccca 900 caggtgagcg ggcgggacgg cccttctcct ccgggctgta
attagcgctt ggtttaatga 960 cggcttgttt cttttctgtg gctgcgtgaa
agccttgagg ggctccggga gggccctttg 1020 tgcgggggga gcggctcggg
gctgtccgcg gggggacggc tgccttcggg ggggacgggg 1080 cagggcgggg
ttcggcttct ggcgtgtgac cggcggctct agcagcctct gctaaccatg 1140
ttcatgcctt cttctttttc ctacagctcc tgggcaacgt gctggttatt gtgctgtctc
1200 atcattttgg caaagaatta agcttgagct cgcgatccgc agccatgact
gagacattgc 1260 caccggtaac ggaatcggct gttgcgctac aggcggaggt
gacccagagg gagctgttcg 1320 agttcgttct caacgacccc ctcctcgcca
gttcgctgta tattaatatc gcactggcag 1380 ggctgtcgat actgcttttc
gtgttcatga cgcgcggact cgacgaccca cgggcgaaac 1440 tcatcgccgt
ttcgacgatt ttggtgccgg tggtctctat cgcgagctac accggccttg 1500
catcggggct caccatcagc gtcctcgaga tgccagccgg ccacttcgcc gaggggtcct
1560 cggtgatgct cggcggcgaa gaggtagacg gcgtcgtgac gatgtggggc
cgctatctga 1620 cgtgggccct ttcgacaccg atgatactgc tggcgcttgg
gctgcttgct ggctctaacg 1680 ccacgaagct ctttaccgcc atcaccttcg
acatcgcgat gtgtgtcacc ggcctcgcag 1740 ccgcgctgac gacctcttcg
cacctgatgc ggtggttctg gtacgccatc agttgtgcgt 1800 gtttcctcgt
cgtcctctac atcctgctcg tcgagtgggc acaggacgcc aaggctgccg 1860
gtactgcgga tatgttcaat acgctgaagc tgctgaccgt tgtcatgtgg ctcggctacc
1920 ccatcgtgtg ggcactcggc gttgagggca tcgccgttct tccggtcgga
gtcacgtcgt 1980 ggggatacag cttcctcgac atcgtcgcga agtacatctt
cgcgttcctg ctgctcaact 2040 acctcacgtc gaacgagagc gtcgtctccg
gctcgatact cgacgtgccg tccgcgtcgg 2100 gcactcccgc tgacgacgaa
ttcggaggcg gaggtggagc tagcgtggtt cttcggaccg 2160 cctgtccccc
aaaaaagagg ctcctgagct tcagggacgt ggacttcgag gaggacagcg 2220
attacacact agctatgagg aggtcacctg atgatgttcc cttaatgaca cccaacacca
2280 ttacaatgta actcgagtct agacgtggta ccgataatca acctctggat
tacaaaattt 2340 gtgaaagatt gactggtatt cttaactatg ttgctccttt
tacgctatgt ggatacgctg 2400 ctttaatgcc tttgtatcat gctattgctt
cccgtatggc tttcattttc tcctccttgt 2460 ataaatcctg gttgctgtct
ctttatgagg agttgtggcc cgttgtcagg caacgtggcg 2520 tggtgtgcac
tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc 2580
agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
2640 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
tccgtggtgt 2700 tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg
tgttgccacc tggattctgc 2760 gcgggacgtc cttctgctac gtcccttcgg
ccctcaatcc agcggacctt ccttcccgcg 2820 gcctgctgcc ggctctgcgg
cctcttccgc gtcttcgcct tcgccctcag acgagtcgga 2880 tctccctttg
ggccgcctcc ccgcctgatg cggggatcct ctagagtcga gagatctacg 2940
ggtggcatcc ctgtgacccc tccccagtgc ctctcctggc cctggaagtt gccactccag
3000 tgcccaccag ccttgtccta ataaaattaa gttgcatcat tttgtctgac
taggtgtcct 3060 tctataatat tatggggtgg aggggggtgg tatggagcaa
ggggcaagtt gggaagacaa 3120 cctgtagggc ctgcggggtc tattgggaac
caagctggag tgcagtggca caatcttggc 3180 tcactgcaat ctccgcctcc
tgggttcaag cgattctcct gcctcagcct cccgagttgt 3240 tgggattcca
ggcatgcatg accaggctca gctaattttt gtttttttgg tagagacggg 3300
gtttcaccat attggccagg ctggtctcca actcctaatc tcaggtgatc tacccacctt
3360 ggcctcccaa attgctggga ttacaggcgt gaaccactgc tcccttccct
gtccttctga 3420 ttttgtaggt aaccacgtgc ggaccgagcg gccgcagggt
aaccacgtgc ggaccgagcg 3480 gccgcaggaa cccctagtga tggagttggc
cactccctct ctgcgcgctc gctcgctcac 3540 tgaggccggg cgaccaaagg
tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag 3600 cgagcgagcg
cgcagctgcc tgcagg 3626 <210> SEQ ID NO 46 <211> LENGTH:
4244 <212> TYPE: DNA <213> ORGANISM: Artificial
Sequence <220> FEATURE: <223> OTHER INFORMATION: 5'
-ITR-CAG-HaloR-GFP-{MLPH Motif}-WPRE-bGHpolyA-ITR- 3' <400>
SEQUENCE: 46 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag
cccgggcgtc 60 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc
gcgcagagag ggagtggcca 120 actccatcac taggggttcc tgcggccgca
cgcgtgatat cctagttatt aatagtaatc 180 aattacgggg tcattagttc
atagcccata tatggagttc cgcgttacat aacttacggt 240 aaatggcccg
cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta 300
tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
360 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
cccctattga 420 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
tacatgacct tatgggactt 480 tcctacttgg cagtacatct acgtattagt
catcgctatt accatgcatg gtcgaggtga 540 gccccacgtt ctgcttcact
ctccccatct cccccccctc cccaccccca attttgtatt 600 tatttatttt
ttaattattt tgtgcagcga tgggggcggg gggggggggg gggcgcgcgc 660
caggcggggc ggggcggggc gaggggcggg gcggggcgag gcggagaggt gcggcggcag
720 ccaatcagag cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg
cggcggcggc 780 cctataaaaa gcgaagcgcg cggcgggcgg gagtcgctgc
gacgctgcct tcgccccgtg 840 ccccgctccg ccgccgcctc gcgccgcccg
ccccggctct gactgaccgc gttactccca 900 caggtgagcg ggcgggacgg
cccttctcct ccgggctgta attagcgctt ggtttaatga 960 cggcttgttt
cttttctgtg gctgcgtgaa agccttgagg ggctccggga gggccctttg 1020
tgcgggggga gcggctcggg gctgtccgcg gggggacggc tgccttcggg ggggacgggg
1080 cagggcgggg ttcggcttct ggcgtgtgac cggcggctct agcagcctct
gctaaccatg 1140 ttcatgcctt cttctttttc ctacagctcc tgggcaacgt
gctggttatt gtgctgtctc 1200 atcattttgg caaagaatta agcttgagct
cgcgatccgc agccatgact gagacattgc 1260 caccggtaac ggaatcggct
gttgcgctac aggcggaggt gacccagagg gagctgttcg 1320 agttcgttct
caacgacccc ctcctcgcca gttcgctgta tattaatatc gcactggcag 1380
ggctgtcgat actgcttttc gtgttcatga cgcgcggact cgacgaccca cgggcgaaac
1440 tcatcgccgt ttcgacgatt ttggtgccgg tggtctctat cgcgagctac
accggccttg 1500 catcggggct caccatcagc gtcctcgaga tgccagccgg
ccacttcgcc gaggggtcct 1560 cggtgatgct cggcggcgaa gaggtagacg
gcgtcgtgac gatgtggggc cgctatctga 1620 cgtgggccct ttcgacaccg
atgatactgc tggcgcttgg gctgcttgct ggctctaacg 1680 ccacgaagct
ctttaccgcc atcaccttcg acatcgcgat gtgtgtcacc ggcctcgcag 1740
ccgcgctgac gacctcttcg cacctgatgc ggtggttctg gtacgccatc agttgtgcgt
1800 gtttcctcgt cgtcctctac atcctgctcg tcgagtgggc acaggacgcc
aaggctgccg 1860 gtactgcgga tatgttcaat acgctgaagc tgctgaccgt
tgtcatgtgg ctcggctacc 1920 ccatcgtgtg ggcactcggc gttgagggca
tcgccgttct tccggtcgga gtcacgtcgt 1980 ggggatacag cttcctcgac
atcgtcgcga agtacatctt cgcgttcctg ctgctcaact 2040 acctcacgtc
gaacgagagc gtcgtctccg gctcgatact cgacgtgccg tccgcgtcgg 2100
gcactcccgc tgacgacgaa ttcggaggcg gaggtggagc tagcaaagga gaagaactct
2160 tcactggagt tggagttgtc ccaattcttg ttgaattaga tggtgatgtt
aacggccaca 2220 agttctctgt cagtggagag ggtgaaggtg atgcaacata
cggaaaactt accctgaagt 2280 tcatctgcac tactggcaaa ctgcctgttc
catggccaac actagtcact actctgtgct 2340 atggtgttca atgcttttca
agatacccgg atcatatgaa acggcatgac tttttcaaga 2400 gtgccatgcc
cgaaggttat gtacaggaaa ggaccatctt cttcaaagat gacggcaact 2460
acaagacacg tgctgaagtc aagtttgaag gtgataccct tgttaataga atcgagttaa
2520 aaggtattga cttcaaggaa gatggcaaca ttctgggaca caaattggaa
tacaactata 2580 actcacacaa tgtatacatc atggcagaca aacaaaagaa
tggaatcaaa gtgaacttca 2640 agacccgcca caacattgaa gatggaagcg
ttcaactagc agaccattat caacaaaata 2700 ctccaattgg cgatggccct
gtccttttac cagacaacca ttacctgtcc acacaatctg 2760 ccctttcgaa
agatcccaac gaaaagagag accacatggt ccttcttgag tttgtaacag 2820
ctgctgggat tacacatggc atggatgaac tgtacaacag ggaccagcct ctgaacagca
2880 aaaagaaaaa gaggctcctg agcttcaggg acgtggactt cgaggaggac
agcgactaac 2940 tcgagtctag acgtggtacc gataatcaac ctctggatta
caaaatttgt gaaagattga 3000 ctggtattct taactatgtt gctcctttta
cgctatgtgg atacgctgct ttaatgcctt 3060 tgtatcatgc tattgcttcc
cgtatggctt tcattttctc ctccttgtat aaatcctggt 3120 tgctgtctct
ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg 3180
tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
3240 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
tgccttgccc 3300 gctgctggac aggggctcgg ctgttgggca ctgacaattc
cgtggtgttg tcggggaagc 3360 tgacgtcctt tccatggctg ctcgcctgtg
ttgccacctg gattctgcgc gggacgtcct 3420 tctgctacgt cccttcggcc
ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg 3480 ctctgcggcc
tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg 3540
ccgcctcccc gcctgatgcg gggatcctct agagtcgaga gatctacggg tggcatccct
3600 gtgacccctc cccagtgcct ctcctggccc tggaagttgc cactccagtg
cccaccagcc 3660 ttgtcctaat aaaattaagt tgcatcattt tgtctgacta
ggtgtccttc tataatatta 3720 tggggtggag gggggtggta tggagcaagg
ggcaagttgg gaagacaacc tgtagggcct 3780 gcggggtcta ttgggaacca
agctggagtg cagtggcaca atcttggctc actgcaatct 3840 ccgcctcctg
ggttcaagcg attctcctgc ctcagcctcc cgagttgttg ggattccagg 3900
catgcatgac caggctcagc taatttttgt ttttttggta gagacggggt ttcaccatat
3960 tggccaggct ggtctccaac tcctaatctc aggtgatcta cccaccttgg
cctcccaaat 4020 tgctgggatt acaggcgtga accactgctc ccttccctgt
ccttctgatt ttgtaggtaa 4080 ccacgtgcgg accgagcggc cgcaggaacc
cctagtgatg gagttggcca ctccctctct 4140 gcgcgctcgc tcgctcactg
aggccgggcg accaaaggtc gcccgacgcc cgggctttgc 4200 ccgggcggcc
tcagtgagcg agcgagcgcg cagctgcctg cagg 4244 <210> SEQ ID NO 47
<211> LENGTH: 3530 <212> TYPE: DNA <213>
ORGANISM: Artificial Sequence <220> FEATURE: <223>
OTHER INFORMATION: 5' -ITR-CAG-HaloR-{MLPH Motif}-WPRE-bGHpoly
A-ITR- 3' <400> SEQUENCE: 47 cctgcaggca gctgcgcgct cgctcgctca
ctgaggccgc ccgggcaaag cccgggcgtc 60 gggcgacctt tggtcgcccg
gcctcagtga gcgagcgagc gcgcagagag ggagtggcca 120 actccatcac
taggggttcc tgcggccgca cgcgtgatat cctagttatt aatagtaatc 180
aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
240 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
taatgacgta 300 tgttcccata gtaacgccaa tagggacttt ccattgacgt
caatgggtgg agtatttacg 360 gtaaactgcc cacttggcag tacatcaagt
gtatcatatg ccaagtacgc cccctattga 420 cgtcaatgac ggtaaatggc
ccgcctggca ttatgcccag tacatgacct tatgggactt 480 tcctacttgg
cagtacatct acgtattagt catcgctatt accatgcatg gtcgaggtga 540
gccccacgtt ctgcttcact ctccccatct cccccccctc cccaccccca attttgtatt
600 tatttatttt ttaattattt tgtgcagcga tgggggcggg gggggggggg
gggcgcgcgc 660 caggcggggc ggggcggggc gaggggcggg gcggggcgag
gcggagaggt gcggcggcag 720 ccaatcagag cggcgcgctc cgaaagtttc
cttttatggc gaggcggcgg cggcggcggc 780 cctataaaaa gcgaagcgcg
cggcgggcgg gagtcgctgc gacgctgcct tcgccccgtg 840 ccccgctccg
ccgccgcctc gcgccgcccg ccccggctct gactgaccgc gttactccca 900
caggtgagcg ggcgggacgg cccttctcct ccgggctgta attagcgctt ggtttaatga
960 cggcttgttt cttttctgtg gctgcgtgaa agccttgagg ggctccggga
gggccctttg 1020 tgcgggggga gcggctcggg gctgtccgcg gggggacggc
tgccttcggg ggggacgggg 1080 cagggcgggg ttcggcttct ggcgtgtgac
cggcggctct agcagcctct gctaaccatg 1140 ttcatgcctt cttctttttc
ctacagctcc tgggcaacgt gctggttatt gtgctgtctc 1200 atcattttgg
caaagaatta agcttgagct cgcgatccgc agccatgact gagacattgc 1260
caccggtaac ggaatcggct gttgcgctac aggcggaggt gacccagagg gagctgttcg
1320 agttcgttct caacgacccc ctcctcgcca gttcgctgta tattaatatc
gcactggcag 1380 ggctgtcgat actgcttttc gtgttcatga cgcgcggact
cgacgaccca cgggcgaaac 1440 tcatcgccgt ttcgacgatt ttggtgccgg
tggtctctat cgcgagctac accggccttg 1500 catcggggct caccatcagc
gtcctcgaga tgccagccgg ccacttcgcc gaggggtcct 1560 cggtgatgct
cggcggcgaa gaggtagacg gcgtcgtgac gatgtggggc cgctatctga 1620
cgtgggccct ttcgacaccg atgatactgc tggcgcttgg gctgcttgct ggctctaacg
1680 ccacgaagct ctttaccgcc atcaccttcg acatcgcgat gtgtgtcacc
ggcctcgcag 1740 ccgcgctgac gacctcttcg cacctgatgc ggtggttctg
gtacgccatc agttgtgcgt 1800 gtttcctcgt cgtcctctac atcctgctcg
tcgagtgggc acaggacgcc aaggctgccg 1860 gtactgcgga tatgttcaat
acgctgaagc tgctgaccgt tgtcatgtgg ctcggctacc 1920 ccatcgtgtg
ggcactcggc gttgagggca tcgccgttct tccggtcgga gtcacgtcgt 1980
ggggatacag cttcctcgac atcgtcgcga agtacatctt cgcgttcctg ctgctcaact
2040 acctcacgtc gaacgagagc gtcgtctccg gctcgatact cgacgtgccg
tccgcgtcgg 2100 gcactcccgc tgacgacgaa ttcggaggcg gaggtggagc
tagcagggac cagcctctga 2160 acagcaaaaa gaaaaagagg ctcctgagct
tcagggacgt ggacttcgag gaggacagcg 2220 actaactcga gtctagacgt
ggtaccgata atcaacctct ggattacaaa atttgtgaaa 2280 gattgactgg
tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa 2340
tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
2400 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
ggcgtggtgt 2460 gcactgtgtt tgctgacgca acccccactg gttggggcat
tgccaccacc tgtcagctcc 2520 tttccgggac tttcgctttc cccctcccta
ttgccacggc ggaactcatc gccgcctgcc 2580 ttgcccgctg ctggacaggg
gctcggctgt tgggcactga caattccgtg gtgttgtcgg 2640 ggaagctgac
gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga 2700
cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
2760 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
cggatctccc 2820 tttgggccgc ctccccgcct gatgcgggga tcctctagag
tcgagagatc tacgggtggc 2880 atccctgtga cccctcccca gtgcctctcc
tggccctgga agttgccact ccagtgccca 2940 ccagccttgt cctaataaaa
ttaagttgca tcattttgtc tgactaggtg tccttctata 3000 atattatggg
gtggaggggg gtggtatgga gcaaggggca agttgggaag acaacctgta 3060
gggcctgcgg ggtctattgg gaaccaagct ggagtgcagt ggcacaatct tggctcactg
3120 caatctccgc ctcctgggtt caagcgattc tcctgcctca gcctcccgag
ttgttgggat 3180 tccaggcatg catgaccagg ctcagctaat ttttgttttt
ttggtagaga cggggtttca 3240 ccatattggc caggctggtc tccaactcct
aatctcaggt gatctaccca ccttggcctc 3300 ccaaattgct gggattacag
gcgtgaacca ctgctccctt ccctgtcctt ctgattttgt 3360 aggtaaccac
gtgcggaccg agcggccgca ggaaccccta gtgatggagt tggccactcc 3420
ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc gacgcccggg
3480 ctttgcccgg gcggcctcag tgagcgagcg agcgcgcagc tgcctgcagg
3530
1 SEQUENCE LISTING <160> NUMBER OF SEQ ID NOS: 47 <210>
SEQ ID NO 1 <211> LENGTH: 195 <212> TYPE: DNA
<213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: Kv2.1 Cytoplasmic C-terminus sorting
motif <400> SEQUENCE: 1 cagtctcagc ccatcctgaa cactaaggag
atggcccctc agagtaaacc ccctgaggaa 60 ctggaaatga gctccatgcc
atctccagtg gctcctctgc cagctaggac cgagggcgtg 120 attgacatga
gaagcatgtc tagtatcgat agcttcattt cctgcgccac cgacttcccc 180
gaagctacaa ggttt 195 <210> SEQ ID NO 2 <211> LENGTH: 65
<212> TYPE: PRT <213> ORGANISM: Artificial Sequence
<220> FEATURE: <223> OTHER INFORMATION: Kv2.1
Cytoplasmic C-terminus sorting motif <400> SEQUENCE: 2 Gln
Ser Gln Pro Ile Leu Asn Thr Lys Glu Met Ala Pro Gln Ser Lys 1 5 10
15 Pro Pro Glu Glu Leu Glu Met Ser Ser Met Pro Ser Pro Val Ala Pro
20 25 30 Leu Pro Ala Arg Thr Glu Gly Val Ile Asp Met Arg Ser Met
Ser Ser 35 40 45 Ile Asp Ser Phe Ile Ser Cys Ala Thr Asp Phe Pro
Glu Ala Thr Arg 50 55 60 Phe 65 <210> SEQ ID NO 3 <211>
LENGTH: 81 <212> TYPE: DNA <213> ORGANISM: Artificial
Sequence <220> FEATURE: <223> OTHER INFORMATION: Nav1.6
Ankyrin binding domain sorting motif <400> SEQUENCE: 3
accgtgaggg tgcccatcgc cgtgggcgag agcgacttcg agaacctgaa caccgaggac
60 gtgagcagcg agagcgaccc c 81 <210> SEQ ID NO 4 <211>
LENGTH: 27 <212> TYPE: PRT <213> ORGANISM: Artificial
Sequence <220> FEATURE: <223> OTHER INFORMATION: Nav1.6
Ankyrin binding domain sorting motif <400> SEQUENCE: 4 Thr
Val Arg Val Pro Ile Ala Val Gly Glu Ser Asp Phe Glu Asn Leu 1 5 10
15 Asn Thr Glu Asp Val Ser Ser Glu Ser Asp Pro 20 25 <210>
SEQ ID NO 5 <211> LENGTH: 96 <212> TYPE: DNA
<213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: NLG-1 Cytoplasmic C-terminal sorting
motif <400> SEQUENCE: 5 gtggtgctga ggactgcctg cccccctgac
tacaccctgg ctatgaggag aagcccagac 60 gatgtgcccc tgatgacccc
caacaccatc acaatg 96 <210> SEQ ID NO 6 <211> LENGTH: 32
<212> TYPE: PRT <213> ORGANISM: Artificial Sequence
<220> FEATURE: <223> OTHER INFORMATION: NLG-1
Cytoplasmic C-terminal sorting motif <400> SEQUENCE: 6 Val
Val Leu Arg Thr Ala Cys Pro Pro Asp Tyr Thr Leu Ala Met Arg 1 5 10
15 Arg Ser Pro Asp Asp Val Pro Leu Met Thr Pro Asn Thr Ile Thr Met
20 25 30 <210> SEQ ID NO 7 <211> LENGTH: 78 <212>
TYPE: DNA <213> ORGANISM: Artificial Sequence <220>
FEATURE: <223> OTHER INFORMATION: MLPH Myosin binding domain
sorting motif <400> SEQUENCE: 7 agggaccagc ctctgaacag
caaaaagaaa aagaggctcc tgagcttcag ggacgtggac 60 ttcgaggagg acagcgac
78 <210> SEQ ID NO 8 <211> LENGTH: 26 <212> TYPE:
PRT <213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: MLPH Myosin binding domain sorting
motif <400> SEQUENCE: 8 Arg Asp Gln Pro Leu Asn Ser Lys Lys
Lys Lys Arg Leu Leu Ser Phe 1 5 10 15 Arg Asp Val Asp Phe Glu Glu
Asp Ser Asp 20 25 <210> SEQ ID NO 9 <211> LENGTH: 141
<212> TYPE: DNA <213> ORGANISM: Artificial Sequence
<220> FEATURE: <223> OTHER INFORMATION: nAchR
Tyrosine-Dileucine sorting motif <400> SEQUENCE: 9 ggcgaggaca
aggtgcggcc cgcctgtcag cacaagcctc ggcggtgcag cctggccagc 60
gtggagctga gcgccggcgc cggcccaccc accagcaacg gcaacctgct gtacatcggc
120 ttcagaggcc tggagggcat g 141 <210> SEQ ID NO 10
<211> LENGTH: 47 <212> TYPE: PRT <213> ORGANISM:
Artificial Sequence <220> FEATURE: <223> OTHER
INFORMATION: nAchR Tyrosine-Dileucine sorting motif <400>
SEQUENCE: 10 Gly Glu Asp Lys Val Arg Pro Ala Cys Gln His Lys Pro
Arg Arg Cys 1 5 10 15 Ala Leu Ala Ser Val Glu Leu Ser Ala Gly Ala
Gly Pro Pro Thr Ser 20 25 30 Asn Gly Asn Leu Leu Tyr Ile Gly Phe
Arg Gly Leu Glu Gly Met 35 40 45 <210> SEQ ID NO 11
<211> LENGTH: 48 <212> TYPE: DNA <213> ORGANISM:
Artificial Sequence <220> FEATURE: <223> OTHER
INFORMATION: Kv4.2 Dileucine sorting motif <400> SEQUENCE: 11
ttcgagcagc agcaccacca cctgctgcac tgcctggaga agaccacc 48 <210>
SEQ ID NO 12 <211> LENGTH: 16 <212> TYPE: PRT
<213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: Kv4.2 Dileucine sorting motif
<400> SEQUENCE: 12 Phe Glu Gln Gln His His His Leu Leu His
Cys Leu Glu Lys Thr Thr 1 5 10 15 <210> SEQ ID NO 13
<211> LENGTH: 192 <212> TYPE: DNA <213> ORGANISM:
Artificial Sequence <220> FEATURE: <223> OTHER
INFORMATION: TLCN Phenylalanine-based sorting motif <400>
SEQUENCE: 13 cagagcacag cctgcaaaaa gggcgagtac aacgtgcagg aagctgagag
ctctggcgaa 60 gccgtgtgtc tgaacggcgc cggaggcggt gccggcggag
ctgccggcgc tgagggtggc 120 cctgaggccg ctggaggtgc cgctgagagc
cccgctgagg gcgaagtctt tgccatccag 180 ctgacatctg ct 192 <210>
SEQ ID NO 14 <211> LENGTH: 64 <212> TYPE: PRT
<213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: TLCN Phenylalanine-based sorting
motif <400> SEQUENCE: 14 Gln Ser Thr Ala Cys Lys Lys Gly Glu
Tyr Asn Val Gln Glu Ala Glu 1 5 10 15 Ser Ser Gly Glu Ala Val Cys
Leu Asn Gly Ala Gly Gly Gly Ala Gly 20 25 30 Gly Ala Ala Gly Ala
Glu Gly Gly Pro Glu Ala Ala Gly Gly Ala Ala 35 40 45 Glu Ser Pro
Ala Glu Gly Glu Val Phe Ala Ile Gln Leu Thr Ser Ala 50 55 60
<210> SEQ ID NO 15 <211> LENGTH: 117 <212> TYPE:
DNA <213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: AMPAR Cytoplasmic C-terminal sorting
motif <400> SEQUENCE: 15
gagttctgct acaagagcag gtccgaatct aagagaatga aaggcttttg tctgatcccc
60 cagcagagca tcaacgaggc cattcggacc agtacactgc ctcgcaatag cggagct
117 <210> SEQ ID NO 16 <211> LENGTH: 39 <212>
TYPE: PRT <213> ORGANISM: Artificial Sequence <220>
FEATURE: <223> OTHER INFORMATION: AMPAR Cytoplasmic
C-terminal sorting motif <400> SEQUENCE: 16 Glu Phe Cys Tyr
Lys Ser Arg Ser Glu Ser Lys Arg Met Lys Gly Phe 1 5 10 15 Cys Leu
Ile Pro Gln Gln Ser Ile Asn Glu Ala Ile Arg Thr Ser Thr 20 25 30
Leu Pro Arg Asn Ser Gly Ala 35 <210> SEQ ID NO 17 <211>
LENGTH: 141 <212> TYPE: DNA <213> ORGANISM: Artificial
Sequence <220> FEATURE: <223> OTHER INFORMATION: AAV2
5' ITR <400> SEQUENCE: 17 cctgcaggca gctgcgcgct cgctcgctca
ctgaggccgc ccgggcaaag cccgggcgtc 60 gggcgacctt tggtcgcccg
gcctcagtga gcgagcgagc gcgcagagag ggagtggcca 120 actccatcac
taggggttcc t 141 <210> SEQ ID NO 18 <211> LENGTH: 141
<212> TYPE: DNA <213> ORGANISM: Artificial Sequence
<220> FEATURE: <223> OTHER INFORMATION: AAV2 3' ITR
<400> SEQUENCE: 18 aggaacccct agtgatggag ttggccactc
cctctctgcg cgctcgctcg ctcactgagg 60 ccgggcgacc aaaggtcgcc
cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc 120 gagcgcgcag
ctgcctgcag g 141 <210> SEQ ID NO 19 <400> SEQUENCE: 19
000 <210> SEQ ID NO 20 <400> SEQUENCE: 20 000
<210> SEQ ID NO 21 <211> LENGTH: 945 <212> TYPE:
DNA <213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: ChR2 <400> SEQUENCE: 21
atggattatg gaggcgccct gagtgccgtt gggcgcgagc tgctatttgt aacgaaccca
60 gtagtcgtca atggctctgt acttgtgcct gaggaccagt gttactgcgc
gggctggatt 120 gagtcgcgtg gcacaaacgg tgcccaaacg gcgtcgaacg
tgctgcaatg gcttgctgct 180 ggcttctcca tcctactgct tatgttttac
gcctaccaaa catggaagtc aacctgcggc 240 tgggaggaga tctatgtgtg
cgctatcgag atggtcaagg tgattcttga gttcttcttc 300 gagtttaaga
acccgtccat gctgtatcta gccacaggcc accgcgtcca gtggttgcgt 360
tacgccgagt ggcttctcac ctgcccggtc attctcattc acctgtcaaa cctgacgggc
420 ttgtccaacg actacagcag gcgcactatg ggtctgcttg tgtctgatat
tggcacaatt 480 gtgtggggcg ccacttccgc tatggccacc ggatacgtca
aggtcatctt cttctgcctg 540 ggtctgtgtt atggtgctaa cacgttcttt
cacgctgcca aggcctacat cgagggttac 600 cataccgtgc cgaagggccg
gtgtcgccag gtggtgactg gcatggcttg gctcttcttc 660 gtatcatggg
gtatgttccc catcctgttc atcctcggcc ccgagggctt cggcgtcctg 720
agcgtgtacg gctccaccgt cggccacacc atcattgacc tgatgtcgaa gaactgctgg
780 ggtctgctcg gccactacct gcgcgtgctg atccacgagc atatcctcat
ccacggcgac 840 attcgcaaga ccaccaaatt gaacattggt ggcactgaga
ttgaggtcga gacgctggtg 900 gaggacgagg ccgaggctgg cgcggtcaac
aagggcaccg gcaag 945 <210> SEQ ID NO 22 <400> SEQUENCE:
22 000 <210> SEQ ID NO 23 <211> LENGTH: 873 <212>
TYPE: DNA <213> ORGANISM: Artificial Sequence <220>
FEATURE: <223> OTHER INFORMATION: HaloR <400> SEQUENCE:
23 atgactgaga cattgccacc ggtaacggaa tcggctgttg cgctacaggc
ggaggtgacc 60 cagagggagc tgttcgagtt cgttctcaac gaccccctcc
tcgccagttc gctgtatatt 120 aatatcgcac tggcagggct gtcgatactg
cttttcgtgt tcatgacgcg cggactcgac 180 gacccacggg cgaaactcat
cgccgtttcg acgattttgg tgccggtggt ctctatcgcg 240 agctacaccg
gccttgcatc ggggctcacc atcagcgtcc tcgagatgcc agccggccac 300
ttcgccgagg ggtcctcggt gatgctcggc ggcgaagagg tagacggcgt cgtgacgatg
360 tggggccgct atctgacgtg ggccctttcg acaccgatga tactgctggc
gcttgggctg 420 cttgctggct ctaacgccac gaagctcttt accgccatca
ccttcgacat cgcgatgtgt 480 gtcaccggcc tcgcagccgc gctgacgacc
tcttcgcacc tgatgcggtg gttctggtac 540 gccatcagtt gtgcgtgttt
cctcgtcgtc ctctacatcc tgctcgtcga gtgggcacag 600 gacgccaagg
ctgccggtac tgcggatatg ttcaatacgc tgaagctgct gaccgttgtc 660
atgtggctcg gctaccccat cgtgtgggca ctcggcgttg agggcatcgc cgttcttccg
720 gtcggagtca cgtcgtgggg atacagcttc ctcgacatcg tcgcgaagta
catcttcgcg 780 ttcctgctgc tcaactacct cacgtcgaac gagagcgtcg
tctccggctc gatactcgac 840 gtgccgtccg cgtcgggcac tcccgctgac gac 873
<210> SEQ ID NO 24 <400> SEQUENCE: 24 000 <210>
SEQ ID NO 25 <211> LENGTH: 708 <212> TYPE: DNA
<213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: GFP <400> SEQUENCE: 25
aaaggagaag aactcttcac tggagttgtc ccaattcttg ttgaattaga tggtgatgtt
60 aacggccaca agttctctgt cagtggagag ggtgaaggtg atgcaacata
cggaaaactt 120 accctgaagt tcatctgcac tactggcaaa ctgcctgttc
catggccaac actagtcact 180 actctgtgct atggtgttca atgcttttca
agatacccgg atcatatgaa acggcatgac 240 tttttcaaga gtgccatgcc
cgaaggttat gtacaggaaa ggaccatctt cttcaaagat 300 gacggcaact
acaagacacg tgctgaagtc aagtttgaag gtgataccct tgttaataga 360
atcgagttaa aaggtattga cttcaaggaa gatggcaaca ttctgggaca caaattggaa
420 tacaactata actcacacaa tgtatacatc atggcagaca aacaaaagaa
tggaatcaaa 480 gtgaacttca agacccgcca caacattgaa gatggaagcg
ttcaactagc agaccattat 540 caacaaaata ctccaattgg cgatggccct
gtccttttac cagacaacca ttacctgtcc 600 acacaatctg ccctttcgaa
agatcccaac gaaaagagag accacatggt ccttcttgag 660 tttgtaacag
ctgctgggat tacacatggc atggatgaac tgtacaac 708 <210> SEQ ID NO
26 <211> LENGTH: 972 <212> TYPE: DNA <213>
ORGANISM: Artificial Sequence <220> FEATURE: <223>
OTHER INFORMATION: CAG promoter <400> SEQUENCE: 26 ctagttatta
atagtaatca attacggggt cattagttca tagcccatat atggagttcc 60
gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat
120 tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
cattgacgtc 180 aatgggtgga gtatttacgg taaactgccc acttggcagt
acatcaagtg tatcatatgc 240 caagtacgcc ccctattgac gtcaatgacg
gtaaatggcc cgcctggcat tatgcccagt 300 acatgacctt atgggacttt
cctacttggc agtacatcta cgtattagtc atcgctatta 360 ccatgcatgg
tcgaggtgag ccccacgttc tgcttcactc tccccatctc ccccccctcc 420
ccacccccaa ttttgtattt atttattttt taattatttt gtgcagcgat gggggcgggg
480 gggggggggg ggcgcgcgcc aggcggggcg gggcggggcg aggggcgggg
cggggcgagg 540 cggagaggtg cggcggcagc caatcagagc ggcgcgctcc
gaaagtttcc ttttatggcg 600 aggcggcggc ggcggcggcc ctataaaaag
cgaagcgcgc ggcgggcggg agtcgctgcg 660 acgctgcctt cgccccgtgc
cccgctccgc cgccgcctcg cgccgcccgc cccggctctg 720 actgaccgcg
ttactcccac aggtgagcgg gcgggacggc ccttctcctc cgggctgtaa 780
ttagcgcttg gtttaatgac ggcttgtttc ttttctgtgg ctgcgtgaaa gccttgaggg
840 gctccgggag ggccctttgt gcggggggag cggctcgggg ctgtccgcgg
ggggacggct 900 gccttcgggg gggacggggc agggcggggt tcggcttctg
gcgtgtgacc ggcggctcta 960 gcagcctctg ct 972 <210> SEQ ID NO
27 <211> LENGTH: 597 <212> TYPE: DNA <213>
ORGANISM: Artificial Sequence <220> FEATURE: <223>
OTHER INFORMATION: Posttranscriptional regulatory element WPRE
<400> SEQUENCE: 27 gataatcaac ctctggatta caaaatttgt
gaaagattga ctggtattct taactatgtt 60 gctcctttta cgctatgtgg
atacgctgct ttaatgcctt tgtatcatgc tattgcttcc 120 cgtatggctt
tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag 180
ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
240 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
tttccccctc 300 cctattgcca cggcggaact catcgccgcc tgccttgccc
gctgctggac aggggctcgg 360 ctgttgggca ctgacaattc cgtggtgttg
tcggggaagc tgacgtcctt tccatggctg 420 ctcgcctgtg ttgccacctg
gattctgcgc gggacgtcct tctgctacgt cccttcggcc 480 ctcaatccag
cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt 540
cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctgat 597
<210> SEQ ID NO 28 <211> LENGTH: 479 <212> TYPE:
DNA <213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: Polyadenylation sequence <400>
SEQUENCE: 28 acgggtggca tccctgtgac ccctccccag tgcctctcct ggccctggaa
gttgccactc 60 cagtgcccac cagccttgtc ctaataaaat taagttgcat
cattttgtct gactaggtgt 120 ccttctataa tattatgggg tggagggggg
tggtatggag caaggggcaa gttgggaaga 180 caacctgtag ggcctgcggg
gtctattggg aaccaagctg gagtgcagtg gcacaatctt 240 ggctcactgc
aatctccgcc tcctgggttc aagcgattct cctgcctcag cctcccgagt 300
tgttgggatt ccaggcatgc atgaccaggc tcagctaatt tttgtttttt tggtagagac
360 ggggtttcac catattggcc aggctggtct ccaactccta atctcaggtg
atctacccac 420 cttggcctcc caaattgctg ggattacagg cgtgaaccac
tgctcccttc cctgtcctt 479 <210> SEQ ID NO 29 <400>
SEQUENCE: 29 000 <210> SEQ ID NO 30 <211> LENGTH: 4232
<212> TYPE: DNA <213> ORGANISM: Artificial Sequence
<220> FEATURE: <223> OTHER INFORMATION: 5'
-ITR-CAG-ChR2-GFP-WPRE-bGHpolyA-ITR- 3' <400> SEQUENCE: 30
cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
60 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag
ggagtggcca 120 actccatcac taggggttcc tgcggccgca cgcgtgatat
cctagttatt aatagtaatc 180 aattacgggg tcattagttc atagcccata
tatggagttc cgcgttacat aacttacggt 240 aaatggcccg cctggctgac
cgcccaacga cccccgccca ttgacgtcaa taatgacgta 300 tgttcccata
gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg 360
gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
420 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
tatgggactt 480 tcctacttgg cagtacatct acgtattagt catcgctatt
accatgcatg gtcgaggtga 540 gccccacgtt ctgcttcact ctccccatct
cccccccctc cccaccccca attttgtatt 600 tatttatttt ttaattattt
tgtgcagcga tgggggcggg gggggggggg gggcgcgcgc 660 caggcggggc
ggggcggggc gaggggcggg gcggggcgag gcggagaggt gcggcggcag 720
ccaatcagag cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg cggcggcggc
780 cctataaaaa gcgaagcgcg cggcgggcgg gagtcgctgc gacgctgcct
tcgccccgtg 840 ccccgctccg ccgccgcctc gcgccgcccg ccccggctct
gactgaccgc gttactccca 900 caggtgagcg ggcgggacgg cccttctcct
ccgggctgta attagcgctt ggtttaatga 960 cggcttgttt cttttctgtg
gctgcgtgaa agccttgagg ggctccggga gggccctttg 1020 tgcgggggga
gcggctcggg gctgtccgcg gggggacggc tgccttcggg ggggacgggg 1080
cagggcgggg ttcggcttct ggcgtgtgac cggcggctct agcagcctct gctaaccatg
1140 ttcatgcctt cttctttttc ctacagctcc tgggcaacgt gctggttatt
gtgctgtctc 1200 atcattttgg caaagaatta agcttgagct cgcgatccgc
agccatggat tatggaggcg 1260 ccctgagtgc cgttgggcgc gagctgctat
ttgtaacgaa cccagtagtc gtcaatggct 1320 ctgtacttgt gcctgaggac
cagtgttact gcgcgggctg gattgagtcg cgtggcacaa 1380 acggtgccca
aacggcgtcg aacgtgctgc aatggcttgc tgctggcttc tccatcctac 1440
tgcttatgtt ttacgcctac caaacatgga agtcaacctg cggctgggag gagatctatg
1500 tgtgcgctat cgagatggtc aaggtgattc ttgagttctt cttcgagttt
aagaacccgt 1560 ccatgctgta tctagccaca ggccaccgcg tccagtggtt
gcgttacgcc gagtggcttc 1620 tcacctgccc ggtcattctc attcacctgt
caaacctgac gggcttgtcc aacgactaca 1680 gcaggcgcac tatgggtctg
cttgtgtctg atattggcac aattgtgtgg ggcgccactt 1740 ccgctatggc
caccggatac gtcaaggtca tcttcttctg cctgggtctg tgttatggtg 1800
ctaacacgtt ctttcacgct gccaaggcct acatcgaggg ttaccatacc gtgccgaagg
1860 gccggtgtcg ccaggtggtg actggcatgg cttggctctt cttcgtatca
tggggtatgt 1920 tccccatcct gttcatcctc ggccccgagg gcttcggcgt
cctgagcgtg tacggctcca 1980 ccgtcggcca caccatcatt gacctgatgt
cgaagaactg ctggggtctg ctcggccact 2040 acctgcgcgt gctgatccac
gagcatatcc tcatccacgg cgacattcgc aagaccacca 2100 aattgaacat
tggtggcact gagattgagg tcgagacgct ggtggaggac gaggccgagg 2160
ctggcgcggt caacaagggc accggcaagg aattcggagg cggaggtgga gctagcaaag
2220 gagaagaact cttcactgga gttgtcccaa ttcttgttga attagatggt
gatgttaacg 2280 gccacaagtt ctctgtcagt ggagagggtg aaggtgatgc
aacatacgga aaacttaccc 2340 tgaagttcat ctgcactact ggcaaactgc
ctgttccatg gccaacacta gtcactactc 2400 tgtgctatgg tgttcaatgc
ttttcaagat acccggatca tatgaaacgg catgactttt 2460 tcaagagtgc
catgcccgaa ggttatgtac aggaaaggac catcttcttc aaagatgacg 2520
gcaactacaa gacacgtgct gaagtcaagt ttgaaggtga tacccttgtt aatagaatcg
2580 agttaaaagg tattgacttc aaggaagatg gcaacattct gggacacaaa
ttggaataca 2640 actataactc acacaatgta tacatcatgg cagacaaaca
aaagaatgga atcaaagtga 2700 acttcaagac ccgccacaac attgaagatg
gaagcgttca actagcagac cattatcaac 2760 aaaatactcc aattggcgat
ggccctgtcc ttttaccaga caaccattac ctgtccacac 2820 aatctgccct
ttcgaaagat cccaacgaaa agagagacca catggtcctt cttgagtttg 2880
taacagctgc tgggattaca catggcatgg atgaactgta caactaactc gagtctagac
2940 gtggtaccga taatcaacct ctggattaca aaatttgtga aagattgact
ggtattctta 3000 actatgttgc tccttttacg ctatgtggat acgctgcttt
aatgcctttg tatcatgcta 3060 ttgcttcccg tatggctttc attttctcct
ccttgtataa atcctggttg ctgtctcttt 3120 atgaggagtt gtggcccgtt
gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg 3180 caacccccac
tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt 3240
tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
3300 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaagctg
acgtcctttc 3360 catggctgct cgcctgtgtt gccacctgga ttctgcgcgg
gacgtccttc tgctacgtcc 3420 cttcggccct caatccagcg gaccttcctt
cccgcggcct gctgccggct ctgcggcctc 3480 ttccgcgtct tcgccttcgc
cctcagacga gtcggatctc cctttgggcc gcctccccgc 3540 ctgatgcggg
gatcctctag agtcgagaga tctacgggtg gcatccctgt gacccctccc 3600
cagtgcctct cctggccctg gaagttgcca ctccagtgcc caccagcctt gtcctaataa
3660 aattaagttg catcattttg tctgactagg tgtccttcta taatattatg
gggtggaggg 3720 gggtggtatg gagcaagggg caagttggga agacaacctg
tagggcctgc ggggtctatt 3780 gggaaccaag ctggagtgca gtggcacaat
cttggctcac tgcaatctcc gcctcctggg 3840 ttcaagcgat tctcctgcct
cagcctcccg agttgttggg attccaggca tgcatgacca 3900 ggctcagcta
atttttgttt ttttggtaga gacggggttt caccatattg gccaggctgg 3960
tctccaactc ctaatctcag gtgatctacc caccttggcc tcccaaattg ctgggattac
4020 aggcgtgaac cactgctccc ttccctgtcc ttctgatttt gtaggtaacc
acgtgcggac 4080 cgagcggccg caggaacccc tagtgatgga gttggccact
ccctctctgc gcgctcgctc 4140 gctcactgag gccgggcgac caaaggtcgc
ccgacgcccg ggctttgccc gggcggcctc 4200 agtgagcgag cgagcgcgca
gctgcctgca gg 4232 <210> SEQ ID NO 31 <211> LENGTH:
3488 <212> TYPE: DNA <213> ORGANISM: Artificial
Sequence <220> FEATURE: <223> OTHER INFORMATION: 5'
-ITR-CAG-ChR2-WPRE-bGHpolyA-ITR- 3' <400> SEQUENCE: 31
cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
60 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag
ggagtggcca 120 actccatcac taggggttcc tgcggccgca cgcgtgatat
cctagttatt aatagtaatc 180 aattacgggg tcattagttc atagcccata
tatggagttc cgcgttacat aacttacggt 240 aaatggcccg cctggctgac
cgcccaacga cccccgccca ttgacgtcaa taatgacgta 300 tgttcccata
gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg 360
gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
420 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
tatgggactt 480 tcctacttgg cagtacatct acgtattagt catcgctatt
accatgcatg gtcgaggtga 540 gccccacgtt ctgcttcact ctccccatct
cccccccctc cccaccccca attttgtatt 600 tatttatttt ttaattattt
tgtgcagcga tgggggcggg gggggggggg gggcgcgcgc 660 caggcggggc
ggggcggggc gaggggcggg gcggggcgag gcggagaggt gcggcggcag 720
ccaatcagag cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg cggcggcggc
780 cctataaaaa gcgaagcgcg cggcgggcgg gagtcgctgc gacgctgcct
tcgccccgtg 840 ccccgctccg ccgccgcctc gcgccgcccg ccccggctct
gactgaccgc gttactccca 900 caggtgagcg ggcgggacgg cccttctcct
ccgggctgta attagcgctt ggtttaatga 960 cggcttgttt cttttctgtg
gctgcgtgaa agccttgagg ggctccggga gggccctttg 1020
tgcgggggga gcggctcggg gctgtccgcg gggggacggc tgccttcggg ggggacgggg
1080 cagggcgggg ttcggcttct ggcgtgtgac cggcggctct agcagcctct
gctaaccatg 1140 ttcatgcctt cttctttttc ctacagctcc tgggcaacgt
gctggttatt gtgctgtctc 1200 atcattttgg caaagaatta agcttgagct
cgcgatccgc agccatggat tatggaggcg 1260 ccctgagtgc cgttgggcgc
gagctgctat ttgtaacgaa cccagtagtc gtcaatggct 1320 ctgtacttgt
gcctgaggac cagtgttact gcgcgggctg gattgagtcg cgtggcacaa 1380
acggtgccca aacggcgtcg aacgtgctgc aatggcttgc tgctggcttc tccatcctac
1440 tgcttatgtt ttacgcctac caaacatgga agtcaacctg cggctgggag
gagatctatg 1500 tgtgcgctat cgagatggtc aaggtgattc ttgagttctt
cttcgagttt aagaacccgt 1560 ccatgctgta tctagccaca ggccaccgcg
tccagtggtt gcgttacgcc gagtggcttc 1620 tcacctgccc ggtcattctc
attcacctgt caaacctgac gggcttgtcc aacgactaca 1680 gcaggcgcac
tatgggtctg cttgtgtctg atattggcac aattgtgtgg ggcgccactt 1740
ccgctatggc caccggatac gtcaaggtca tcttcttctg cctgggtctg tgttatggtg
1800 ctaacacgtt ctttcacgct gccaaggcct acatcgaggg ttaccatacc
gtgccgaagg 1860 gccggtgtcg ccaggtggtg actggcatgg cttggctctt
cttcgtatca tggggtatgt 1920 tccccatcct gttcatcctc ggccccgagg
gcttcggcgt cctgagcgtg tacggctcca 1980 ccgtcggcca caccatcatt
gacctgatgt cgaagaactg ctggggtctg ctcggccact 2040 acctgcgcgt
gctgatccac gagcatatcc tcatccacgg cgacattcgc aagaccacca 2100
aattgaacat tggtggcact gagattgagg tcgagacgct ggtggaggac gaggccgagg
2160 ctggcgcggt caacaagggc accggcaagg aattcggagg cggaggtgga
gctagctaac 2220 tcgagtctag acgtggtacc ttgactggta ttcttaacta
tgttgctcct tttacgctat 2280 gtggatacgc tgctttaatg cctttgtatc
atgctattgc ttcccgtatg gctttcattt 2340 tctcctcctt gtataaatcc
tggttgctgt ctctttatga ggagttgtgg cccgttgtca 2400 ggcaacgtgg
cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg 2460
ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
2520 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
ggcactgaca 2580 attccgtggt gttgtcgggg aagctgacgt cctttccatg
gctgctcgcc tgtgttgcca 2640 cctggattct gcgcgggacg tccttctgct
acgtcccttc ggccctcaat ccagcggacc 2700 ttccttcccg cggcctgctg
ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc 2760 agacgagtcg
gatctccctt tgggccgcct ccccgcctga tgcggggatc ctctagagtc 2820
gagagatcta cgggtggcat ccctgtgacc cctccccagt gcctctcctg gccctggaag
2880 ttgccactcc agtgcccacc agccttgtcc taataaaatt aagttgcatc
attttgtctg 2940 actaggtgtc cttctataat attatggggt ggaggggggt
ggtatggagc aaggggcaag 3000 ttgggaagac aacctgtagg gcctgcgggg
tctattggga accaagctgg agtgcagtgg 3060 cacaatcttg gctcactgca
atctccgcct cctgggttca agcgattctc ctgcctcagc 3120 ctcccgagtt
gttgggattc caggcatgca tgaccaggct cagctaattt ttgttttttt 3180
ggtagagacg gggtttcacc atattggcca ggctggtctc caactcctaa tctcaggtga
3240 tctacccacc ttggcctccc aaattgctgg gattacaggc gtgaaccact
gctcccttcc 3300 ctgtccttct gattttgtag gtaaccacgt gcggaccgag
cggccgcagg aacccctagt 3360 gatggagttg gccactccct ctctgcgcgc
tcgctcgctc actgaggccg ggcgaccaaa 3420 ggtcgcccga cgcccgggct
ttgcccgggc ggcctcagtg agcgagcgag cgcgcagctg 3480 cctgcagg 3488
<210> SEQ ID NO 32 <211> LENGTH: 4427 <212> TYPE:
DNA <213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: 5'- ITR-CAG-ChR2-GFP-{Kv2.1
Motif}-WPRE-bGHpolyA-ITR- 3' <400> SEQUENCE: 32 cctgcaggca
gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc 60
gggcgaccgg tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
120 actccatcac taggggttcc tgcggccgca cgcgtgatat cctagttatt
aatagtaatc 180 aattacgggg tcattagttc atagcccata tatggagttc
cgcgttacat aacttacggt 240 aaatggcccg cctggctgac cgcccaacga
cccccgccca ttgacgtcaa taatgacgta 300 tgttcccata gtaacgccaa
tagggacttt ccattgacgt caatgggtgg agtatttacg 360 gtaaactgcc
cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga 420
cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
480 tcctacttgg cagtacatct acgtattagt catcgctatt accatgcatg
gtcgaggtga 540 gccccacgtt ctgcttcact ctccccatct cccccccctc
cccaccccca attttgtatt 600 tatttatttt ttaattattt tgtgcagcga
tgggggcggg gggggggggg gggcgcgcgc 660 caggcggggc ggggcggggc
gaggggcggg gcggggcgag gcggagaggt gcggcggcag 720 ccaatcagag
cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg cggcggcggc 780
cctataaaaa gcgaagcgcg cggcgggcgg gagtcgctgc gacgctgcct tcgccccgtg
840 ccccgctccg ccgccgcctc gcgccgcccg ccccggctct gactgaccgc
gttactccca 900 caggtgagcg ggcgggacgg cccttctcct ccgggctgta
attagcgctt ggtttaatga 960 cggcttgttt cttttctgtg gctgcgtgaa
agccttgagg ggctccggga gggccctttg 1020 tgcgggggga gcggctcggg
gctgtccgcg gggggacggc tgccttcggg ggggacgggg 1080 cagggcgggg
ttcggcttct ggcgtgtgac cggcggctct agcagcctct gctaaccatg 1140
ttcatgcctt cttctttttc ctacagctcc tgggcaacgt gctggttatt gtgctgtctc
1200 atcattttgg caaagaatta agcttgagct cgcgatccgc agccatggat
tatggaggcg 1260 ccctgagtgc cgttgggcgc gagctgctat ttgtaacgaa
cccagtagtc gtcaatggct 1320 ctgtacttgt gcctgaggac cagtgttact
gcgcgggctg gattgagtcg cgtggcacaa 1380 acggtgccca aacggcgtcg
aacgtgctgc aatggcttgc tgctggcttc tccatcctac 1440 tgcttatgtt
ttacgcctac caaacatgga agtcaacctg cggctgggag gagatctatg 1500
tgtgcgctat cgagatggtc aaggtgattc ttgagttctt cttcgagttt aagaacccgt
1560 ccatgctgta tctagccaca ggccaccgcg tccagtggtt gcgttacgcc
gagtggcttc 1620 tcacctgccc ggtcattctc attcacctgt caaacctgac
gggcttgtcc aacgactaca 1680 gcaggcgcac tatgggtctg cttgtgtctg
atattggcac aattgtgtgg ggcgccactt 1740 ccgctatggc caccggatac
gtcaaggtca tcttcttctg cctgggtctg tgttatggtg 1800 ctaacacgtt
ctttcacgct gccaaggcct acatcgaggg ttaccatacc gtgccgaagg 1860
gccggtgtcg ccaggtggtg actggcatgg cttggctctt cttcgtatca tggggtatgt
1920 tccccatcct gttcatcctc ggccccgagg gcttcggcgt cctgagcgtg
tacggctcca 1980 ccgtcggcca caccatcatt gacctgatgt cgaagaactg
ctggggtctg ctcggccact 2040 acctgcgcgt gctgatccac gagcatatcc
tcatccacgg cgacattcgc aagaccacca 2100 aattgaacat tggtggcact
gagattgagg tcgagacgct ggtggaggac gaggccgagg 2160 ctggcgcggt
caacaagggc accggcaagg aattcggagg cggaggtgga gctagcaaag 2220
gagaagaact cttcactgga gttgtcccaa ttcttgttga attagatggt gatgttaacg
2280 gccacaagtt ctctgtcagt ggagagggtg aaggtgatgc aacatacgga
aaacttaccc 2340 tgaagttcat ctgcactact ggcaaactgc ctgttccatg
gccaacacta gtcactactc 2400 tgtgctatgg tgttcaatgc ttttcaagat
acccggatca tatgaaacgg catgactttt 2460 tcaagagtgc catgcccgaa
ggttatgtac aggaaaggac catcttcttc aaagatgacg 2520 gcaactacaa
gacacgtgct gaagtcaagt ttgaaggtga tacccttgtt aatagaatcg 2580
agttaaaagg tattgacttc aaggaagatg gcaacattct gggacacaaa ttggaataca
2640 actataactc acacaatgta tacatcatgg cagacaaaca aaagaatgga
atcaaagtga 2700 acttcaagac ccgccacaac attgaagatg gaagcgttca
actagcagac cattatcaac 2760 aaaatactcc aattggcgat ggccctgtcc
ttttaccaga caaccattac ctgtccacac 2820 aatctgccct ttcgaaagat
cccaacgaaa agagagacca catggtcctt cttgagtttg 2880 taacagctgc
tgggattaca catggcatgg atgaactgta caaccagtct cagcccatcc 2940
tgaacactaa ggagatggcc cctcagagta aaccccctga ggaactggaa atgagctcca
3000 tgccatctcc agtggctcct ctgccagcta ggaccgaggg cgtgattgac
atgagaagca 3060 tgtctagtat cgatagcttc atttcctgcg ccaccgactt
ccccgaagct acaaggtttt 3120 aactcgagtc tagacgtggt accgataatc
aacctctgga ttacaaaatt tgtgaaagat 3180 tgactggtat tcttaactat
gttgctcctt ttacgctatg tggatacgct gctttaatgc 3240 ctttgtatca
tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct 3300
ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
3360 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
cagctccttt 3420 ccgggacttt cgctttcccc ctccctattg ccacggcgga
actcatcgcc gcctgccttg 3480 cccgctgctg gacaggggct cggctgttgg
gcactgacaa ttccgtggtg ttgtcgggga 3540 agctgacgtc ctttccatgg
ctgctcgcct gtgttgccac ctggattctg cgcgggacgt 3600 ccttctgcta
cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc 3660
cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt
3720 gggccgcctc cccgcctgat gcggggatcc tctagagtcg agagatctac
gggtggcatc 3780 cctgtgaccc ctccccagtg cctctcctgg ccctggaagt
tgccactcca gtgcccacca 3840 gccttgtcct aataaaatta agttgcatca
ttttgtctga ctaggtgtcc ttctataata 3900 ttatggggtg gaggggggtg
gtatggagca aggggcaagt tgggaagaca acctgtaggg 3960 cctgcggggt
ctattgggaa ccaagctgga gtgcagtggc acaatcttgg ctcactgcaa 4020
tctccgcctc ctgggttcaa gcgattctcc tgcctcagcc tcccgagttg ttgggattcc
4080 aggcatgcat gaccaggctc agctaatttt tgtttttttg gtagagacgg
ggtttcacca 4140 tattggccag gctggtctcc aactcctaat ctcaggtgat
ctacccacct tggcctccca 4200 aattgctggg attacaggcg tgaaccactg
ctcccttccc tgtccttctg attttgtagg 4260 taaccacgtg cggaccgagc
ggccgcagga acccctagtg atggagttgg ccactccctc 4320 tctgcgcgct
cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac gcccgggctt 4380
tgcccgggcg gcctcagtga gcgagcgagc gcgcagctgc ctgcagg 4427
<210> SEQ ID NO 33 <211> LENGTH: 3719 <212> TYPE:
DNA <213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: 5' -ITR-CAG-ChR2-{Kv2 .1
Motif}-WPRE-bGHpolyA-ITR- 3' <400> SEQUENCE: 33 cctgcaggca
gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc 60
gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
120 actccatcac taggggttcc tgcggccgca cgcgtgatat cctagttatt
aatagtaatc 180 aattacgggg tcattagttc atagcccata tatggagttc
cgcgttacat aacttacggt 240 aaatggcccg cctggctgac cgcccaacga
cccccgccca ttgacgtcaa taatgacgta 300 tgttcccata gtaacgccaa
tagggacttt ccattgacgt caatgggtgg agtatttacg 360 gtaaactgcc
cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga 420
cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
480 tcctacttgg cagtacatct acgtattagt catcgctatt accatgcatg
gtcgaggtga 540 gccccacgtt ctgcttcact ctccccatct cccccccctc
cccaccccca attttgtatt 600 tatttatttt ttaattattt tgtgcagcga
tgggggcggg gggggggggg gggcgcgcgc 660 caggcggggc ggggcggggc
gaggggcggg gcggggcgag gcggagaggt gcggcggcag 720 ccaatcagag
cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg cggcggcggc 780
cctataaaaa gcgaagcgcg cggcgggcgg gagtcgctgc gacgctgcct tcgccccgtg
840 ccccgctccg ccgccgcctc gcgccgcccg ccccggctct gactgaccgc
gttactccca 900 caggtgagcg ggcgggacgg cccttctcct ccgggctgta
attagcgctt ggtttaatga 960 cggcttgttt cttttctgtg gctgcgtgaa
agccttgagg ggctccggga gggccctttg 1020 tgcgggggga gcggctcggg
gctgtccgcg gggggacggc tgccttcggg ggggacgggg 1080 cagggcgggg
ttcggcttct ggcgtgtgac cggcggctct agcagcctct gctaaccatg 1140
ttcatgcctt cttctttttc ctacagctcc tgggcaacgt gctggttatt gtgctgtctc
1200 atcattttgg caaagaatta agcttgagct cgcgatccgc agccatggat
tatggaggcg 1260 ccctgagtgc cgttgggcgc gagctgctat ttgtaacgaa
cccagtagtc gtcaatggct 1320 ctgtacttgt gcctgaggac cagtgttact
gcgcgggctg gattgagtcg cgtggcacaa 1380 acggtgccca aacggcgtcg
aacgtgctgc aatggcttgc tgctggcttc tccatcctac 1440 tgcttatgtt
ttacgcctac caaacatgga agtcaacctg cggctgggag gagatctatg 1500
tgtgcgctat cgagatggtc aaggtgattc ttgagttctt cttcgagttt aagaacccgt
1560 ccatgctgta tctagccaca ggccaccgcg tccagtggtt gcgttacgcc
gagtggcttc 1620 tcacctgccc ggtcattctc attcacctgt caaacctgac
gggcttgtcc aacgactaca 1680 gcaggcgcac tatgggtctg cttgtgtctg
atattggcac aattgtgtgg ggcgccactt 1740 ccgctatggc caccggatac
gtcaaggtca tcttcttctg cctgggtctg tgttatggtg 1800 ctaacacgtt
ctttcacgct gccaaggcct acatcgaggg ttaccatacc gtgccgaagg 1860
gccggtgtcg ccaggtggtg actggcatgg cttggctctt cttcgtatca tggggtatgt
1920 tccccatcct gttcatcctc ggccccgagg gcttcggcgt cctgagcgtg
tacggctcca 1980 ccgtcggcca caccatcatt gacctgatgt cgaagaactg
ctggggtctg ctcggccact 2040 acctgcgcgt gctgatccac gagcatatcc
tcatccacgg cgacattcgc aagaccacca 2100 aattgaacat tggtggcact
gagattgagg tcgagacgct ggtggaggac gaggccgagg 2160 ctggcgcggt
caacaagggc accggcaagg aattcggagg cggaggtgga gctagccagt 2220
ctcagcccat cctgaacact aaggagatgg cccctcagag taaaccccct gaggaactgg
2280 aaatgagctc catgccatct ccagtggctc ctctgccagc taggaccgag
ggcgtgattg 2340 acatgagaag catgtctagt atcgatagct tcatttcctg
cgccaccgac ttccccgaag 2400 ctacaaggtt ttaactcgag tctagacgtg
gtaccgataa tcaacctctg gattacaaaa 2460 tttgtgaaag attgactggt
attcttaact atgttgctcc ttttacgcta tgtggatacg 2520 ctgctttaat
gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct 2580
tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
2640 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
gccaccacct 2700 gtcagctcct ttccgggact ttcgctttcc ccctccctat
tgccacggcg gaactcatcg 2760 ccgcctgcct tgcccgctgc tggacagggg
ctcggctgtt gggcactgac aattccgtgg 2820 tgttgtcggg gaagctgacg
tcctttccat ggctgctcgc ctgtgttgcc acctggattc 2880 tgcgcgggac
gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc 2940
gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
3000 ggatctccct ttgggccgcc tccccgcctg atgcggggat cctctagagt
cgagagatct 3060 acgggtggca tccctgtgac ccctccccag tgcctctcct
ggccctggaa gttgccactc 3120 cagtgcccac cagccttgtc ctaataaaat
taagttgcat cattttgtct gactaggtgt 3180 ccttctataa tattatgggg
tggagggggg tggtatggag caaggggcaa gttgggaaga 3240 caacctgtag
ggcctgcggg gtctattggg aaccaagctg gagtgcagtg gcacaatctt 3300
ggctcactgc aatctccgcc tcctgggttc aagcgattct cctgcctcag cctcccgagt
3360 tgttgggatt ccaggcatgc atgaccaggc tcagctaatt tttgtttttt
tggtagagac 3420 ggggtttcac catattggcc aggctggtct ccaactccta
atctcaggtg atctacccac 3480 cttggcctcc caaattgctg ggattacagg
cgtgaaccac tgctcccttc cctgtccttc 3540 tgattttgta ggtaaccacg
tgcggaccga gcggccgcag gaacccctag tgatagagtt 3600 ggccactccc
tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa aagtcgcccg 3660
acgcccgggc tttgcccggg cggcctcagt gagcgagcga gcgcgcagct gcctgcagg
3719 <210> SEQ ID NO 34 <211> LENGTH: 4313 <212>
TYPE: DNA <213> ORGANISM: Artificial Sequence <220>
FEATURE: <223> OTHER INFORMATION: 5'-ITR-CAG-ChR2-GFP-{Nav1.6
Motif}-WPRE-bGHpolyA-ITR- 3' <400> SEQUENCE: 34 cctgcaggca
gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc 60
gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
120 actccatcac taggggttcc tgcggccgca cgcgtgatat cctagttatt
aatagtaatc 180 aattacgggg tcattagttc atagcccata tatggagttc
cgcgttacat aacttacggt 240 aaatggcccg cctggctgac cgcccaacga
cccccgccca ttgacgtcaa taatgacgta 300 tgttcccata gtaacgccaa
tagggacttt ccattgacgt caatgggtgg agtatttacg 360 gtaaactgcc
cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga 420
cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
480 tcctacttgg cagtacatct acgtattagt catcgctatt accatgcatg
gtcgaggtga 540 gccccacgtt ctgcttcact ctccccatct cccccccctc
cccaccccca attttgtatt 600 tatttatttt ttaattattt tgtgcagcga
tgggggcggg gggggggggg gggcgcgcgc 660 caggcggggc ggggcggggc
gaggggcggg gcggggcgag gcggagaggt gcggcggcag 720 ccaatcagag
cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg cggcggcggc 780
cctataaaaa gcgaagcgcg cggcgggcgg gagtcgctgc gacgctgcct tcgccccgtg
840 ccccgctccg ccgccgcctc gcgccgcccg ccccggctct gactgaccgc
gttactccca 900 caggtgagcg ggcgggacgg cccttctcct ccgggctgta
attagcgctt ggtttaatga 960 cggcttgttt cttttctgtg gctgcgtgaa
agccttgagg ggctccggga gggccctttg 1020 tgcgggggga gcggctcggg
gctgtccgcg gggggacggc tgccttcggg ggggacgggg 1080 cagggcgggg
ttcggcttct ggcgtgtgac cggcggctct agcagcctct gctaaccatg 1140
ttcatgcctt cttctttttc ctacagctcc tgggcaacgt gctggttatt gtgctgtctc
1200 atcattttgg caaagaatta agcttgagct cgcgatccgc agccatggat
tatggaggcg 1260 ccctgagtgc cgttgggcgc gagctgctat ttgtaacgaa
cccagtagtc gtcaatggct 1320 ctgtacttgt gcctgaggac cagtgttact
gcgcgggctg gattgagtcg cgtggcacaa 1380 acggtgccca aacggcgtcg
aacgtgctgc aatggcttgc tgctggcttc tccatcctac 1440 tgcttatgtt
ttacgcctac caaacatgga agtcaacctg cggctgggag gagatctatg 1500
tgtgcgctat cgagatggtc aaggtgattc ttgagttctt cttcgagttt aagaacccgt
1560 ccatgctgta tctagccaca ggccaccgcg tccagtggtt gcgttacgcc
gagtggcttc 1620 tcacctgccc ggtcattctc attcacctgt caaacctgac
gggcttgtcc aacgactaca 1680 gcaggcgcac tatgggtctg cttgtgtctg
atattggcac aattgtgtgg ggcgccactt 1740 ccgctatggc caccggatac
gtcaaggtca tcttcttctg cctgggtctg tgttatggtg 1800 ctaacacgtt
ctttcacgct gccaaggcct acatcgaggg ttaccatacc gtgccgaagg 1860
gccggtgtcg ccaggtggtg actggcatgg cttggctctt cttcgtatca tggggtatgt
1920 tccccatcct gttcatcctc ggccccgagg gcttcggcgt cctgagcgtg
tacggctcca 1980 ccgtcggcca caccatcatt gacctgatgt cgaagaactg
ctggggtctg ctcggccact 2040 acctgcgcgt gctgatccac gagcatatcc
tcatccacgg cgacattcgc aagaccacca 2100 aattgaacat tggtggcact
gagattgagg tcgagacgct ggtggaggac gaggccgagg 2160 ctggcgcggt
caacaagggc accggcaagg aattcggagg cggaggtgga gctagcaaag 2220
gagaagaact cttcactgga gttgtcccaa ttcttgttga attagatggt gatgttaacg
2280 gccacaagtt ctctgtcagt ggagagggtg aaggtgatgc aacatacgga
aaacttaccc 2340 tgaagttcat ctgcactact ggcaaactgc ctgttccatg
gccaacacta gtcactactc 2400 tgtgctatgg tgttcaatgc ttttcaagat
acccggatca tatgaaacgg catgactttt 2460 tcaagagtgc catgcccgaa
ggttatgtac aggaaaggac catcttcttc aaagatgacg 2520 gcaactacaa
gacacgtgct gaagtcaagt ttgaaggtga tacccttgtt aatagaatcg 2580
agttaaaagg tattgacttc aaggaagatg gcaacattct gggacacaaa ttggaataca
2640 actataactc acacaatgta tacatcatgg cagacaaaca aaagaatgga
atcaaagtga 2700 acttcaagac ccgccacaac attgaagatg gaagcgttca
actagcagac cattatcaac 2760 aaaatactcc aattggcgat ggccctgtcc
ttttaccaga caaccattac ctgtccacac 2820 aatctgccct ttcgaaagat
cccaacgaaa agagagacca catggtcctt cttgagtttg 2880 taacagctgc
tgggattaca catggcatgg atgaactgta caacaccgtg agggtgccca 2940
tcgccgtggg cgagagcgac ttcgagaacc tgaacaccga ggacgtgagc agcgagagcg
3000 acccctaact cgagtctaga cgtggtaccg ataatcaacc tctggattac
aaaatttgtg 3060 aaagattgac tggtattctt aactatgttg ctccttttac
gctatgtgga tacgctgctt 3120 taatgccttt gtatcatgct attgcttccc
gtatggcttt cattttctcc tccttgtata 3180 aatcctggtt gctgtctctt
tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg 3240 tgtgcactgt
gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc 3300
tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
3360
gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt
3420 cggggaagct gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg
attctgcgcg 3480 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc
ggaccttcct tcccgcggcc 3540 tgctgccggc tctgcggcct cttccgcgtc
ttcgccttcg ccctcagacg agtcggatct 3600 ccctttgggc cgcctccccg
cctgatgcgg ggatcctcta gagtcgagag atctacgggt 3660 ggcatccctg
tgacccctcc ccagtgcctc tcctggccct ggaagttgcc actccagtgc 3720
ccaccagcct tgtcctaata aaattaagtt gcatcatttt gtctgactag gtgtccttct
3780 ataatattat ggggtggagg ggggtggtat ggagcaaggg gcaagttggg
aagacaacct 3840 gtagggcctg cggggtctat tgggaaccaa gctggagtgc
agtggcacaa tcttggctca 3900 ctgcaatctc cgcctcctgg gttcaagcga
ttctcctgcc tcagcctccc gagttgttgg 3960 gattccaggc atgcatgacc
aggctcagct aatttttgtt tttttggtag agacggggtt 4020 tcaccatatt
ggccaggctg gtctccaact cctaatctca ggtgatctac ccaccttggc 4080
ctcccaaatt gctgggatta caggcgtgaa ccactgctcc cttccctgtc cttctgattt
4140 tgtaggtaac cacgtgcgga ccgagcggcc gcaggaaccc ctagtgatgg
agttggccac 4200 tccctctctg cgcgctcgct cgctcactga ggccgggcga
ccaaaggtcg cccgacgccc 4260 gggctttgcc cgggcggcct cagtgagcga
gcgagcgcgc agctgcctgc agg 4313 <210> SEQ ID NO 35 <211>
LENGTH: 3605 <212> TYPE: DNA <213> ORGANISM: Artificial
Sequence <220> FEATURE: <223> OTHER INFORMATION: 5'-
ITR-CAG-ChR2-{Nav1.6Motif}-WPRE-bGHpolyA-ITR-3' <400>
SEQUENCE: 35 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag
cccgggcgtc 60 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc
gcgcagagag ggagtggcca 120 actccatcac taggggttcc tgcggccgca
cgcgtgatat cctagttatt aatagtaatc 180 aattacgggg tcattagttc
atagcccata tatggagttc cgcgttacat aacttacggt 240 aaatggcccg
cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta 300
tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
360 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
cccctattga 420 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
tacatgacct tatgggactt 480 tcctacttgg cagtacatct acgtattagt
catcgctatt accatgcatg gtcgaggtga 540 gccccacgtt ctgcttcact
ctccccatct cccccccctc cccaccccca attttgtatt 600 tatttatttt
ttaattattt tgtgcagcga tgggggcggg gggggggggg gggcgcgcgc 660
caggcggggc ggggcggggc gaggggcggg gcggggcgag gcggagaggt gcggcggcag
720 ccaatcagag cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg
cggcggcggc 780 cctataaaaa gcgaagcgcg cggcgggcgg gagtcgctgc
gacgctgcct tcgccccgtg 840 ccccgctccg ccgccgcctc gcgccgcccg
ccccggctct gactgaccgc gttactccca 900 caggtgagcg ggcgggacgg
cccttctcct ccgggctgta attagcgctt ggtttaatga 960 cggcttgttt
cttttctgtg gctgcgtgaa agccttgagg ggctccggga gggccctttg 1020
tgcgggggga gcggctcggg gctgtccgcg gggggacggc tgccttcggg ggggacgggg
1080 cagggcgggg ttcggcttct ggcgtgtgac cggcggctct agcagcctct
gctaaccatg 1140 ttcatgcctt cttctttttc ctacagctcc tgggcaacgt
gctggttatt gtgctgtctc 1200 atcattttgg caaagaatta agcttgagct
cgcgatccgc agccatggat tatggaggcg 1260 ccctgagtgc cgttgggcgc
gagctgctat ttgtaacgaa cccagtagtc gtcaatggct 1320 ctgtacttgt
gcctgaggac cagtgttact gcgcgggctg gattgagtcg cgtggcacaa 1380
acggtgccca aacggcgtcg aacgtgctgc aatggcttgc tgctggcttc tccatcctac
1440 tgcttatgtt ttacgcctac caaacatgga agtcaacctg cggctgggag
gagatctatg 1500 tgtgcgctat cgagatggtc aaggtgattc ttgagttctt
cttcgagttt aagaacccgt 1560 ccatgctgta tctagccaca ggccaccgcg
tccagtggtt gcgttacgcc gagtggcttc 1620 tcacctgccc ggtcattctc
attcacctgt caaacctgac gggcttgtcc aacgactaca 1680 gcaggcgcac
tatgggtctg cttgtgtctg atattggcac aattgtgtgg ggcgccactt 1740
ccgctatggc caccggatac gtcaaggtca tcttcttctg cctgggtctg tgttatggtg
1800 ctaacacgtt ctttcacgct gccaaggcct acatcgaggg ttaccatacc
gtgccgaagg 1860 gccggtgtcg ccaggtggtg actggcatgg cttggctctt
cttcgtatca tggggtatgt 1920 tccccatcct gttcatcctc ggccccgagg
gcttcggcgt cctgagcgtg tacggctcca 1980 ccgtcggcca caccatcatt
gacctgatgt cgaagaactg ctggggtctg ctcggccact 2040 acctgcgcgt
gctgatccac gagcatatcc tcatccacgg cgacattcgc aagaccacca 2100
aattgaacat tggtggcact gagattgagg tcgagacgct ggtggaggac gaggccgagg
2160 ctggcgcggt caacaagggc accggcaagg aattcggagg cggaggtgga
gctagcaccg 2220 tgagggtgcc catcgccgtg ggcgagagcg acttcgagaa
cctgaacacc gaggacgtga 2280 gcagcgagag cgacccctaa ctcgagtcta
gacgtggtac cgataatcaa cctctggatt 2340 acaaaatttg tgaaagattg
actggtattc ttaactatgt tgctcctttt acgctatgtg 2400 gatacgctgc
tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct 2460
cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc
2520 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
ggcattgcca 2580 ccacctgtca gctcctttcc gggactttcg ctttccccct
ccctattgcc acggcggaac 2640 tcatcgccgc ctgccttgcc cgctgctgga
caggggctcg gctgttgggc actgacaatt 2700 ccgtggtgtt gtcggggaag
ctgacgtcct ttccatggct gctcgcctgt gttgccacct 2760 ggattctgcg
cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc 2820
cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga
2880 cgagtcggat ctccctttgg gccgcctccc cgcctgatgc ggggatcctc
tagagtcgag 2940 agatctacgg gtggcatccc tgtgacccct ccccagtgcc
tctcctggcc ctggaagttg 3000 ccactccagt gcccaccagc cttgtcctaa
taaaattaag ttgcatcatt ttgtctgact 3060 aggtgtcctt ctataatatt
atggggtgga ggggggtggt atggagcaag gggcaagttg 3120 ggaagacaac
ctgtagggcc tgcggggtct attgggaacc aagctggagt gcagtggcac 3180
aatcttggct cactgcaatc tccgcctcct gggttcaagc gattctcctg cctcagcctc
3240 ccgagttgtt gggattccag gcatgcatga ccaggctcag ctaatttttg
tttttttggt 3300 agagacgggg tttcaccata ttggccaggc tggtctccaa
ctcctaatct caggtgatct 3360 acccaccttg gcctcccaaa ttgctgggat
tacaggcgtg aaccactgct cccttccctg 3420 tccttctgat tttgtaggta
accacgtgcg gaccgagcgg ccgcaggaac ccctagtgat 3480 ggagttggcc
actccctctc tgcgcgctcg ctcgctcact gaggccgggc gaccaaaggt 3540
cgcccgacgc ccgggctttg cccgggcggc ctcagtgagc gagcgagcgc gcagctgcct
3600 gcagg 3605 <210> SEQ ID NO 36 <211> LENGTH: 4328
<212> TYPE: DNA <213> ORGANISM: Artificial Sequence
<220> FEATURE: <223> OTHER INFORMATION: 5'-
ITR-CAG-ChR2-GFP-{NLG1 Motif}-WPRE-bGHpolyA-ITR- 3' <400>
SEQUENCE: 36 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag
cccgggcgtc 60 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc
gcgcagagag ggagtggcca 120 actccatcac taggggttcc tgcggccgca
cgcgtgatat cctagttatt aatagtaatc 180 aattacgggg tcattagttc
atagcccata tatggagttc cgcgttacat aacttacggt 240 aaatggcccg
cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta 300
tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
360 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
cccctattga 420 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
tacatgacct tatgggactt 480 tcctacttgg cagtacatct acgtattagt
catcgctatt accatgcatg gtcgaggtga 540 gccccacgtt ctgcttcact
ctccccatct cccccccctc cccaccccca attttgtatt 600 tatttatttt
ttaattattt tgtgcagcga tgggggcggg gggggggggg gggcgcgcgc 660
caggcggggc ggggcggggc gaggggcggg gcggggcgag gcggagaggt gcggcggcag
720 ccaatcagag cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg
cggcggcggc 780 cctataaaaa gcgaagcgcg cggcgggcgg gagtcgctgc
gacgctgcct tcgccccgtg 840 ccccgctccg ccgccgcctc gcgccgcccg
ccccggctct gactgaccgc gttactccca 900 caggtgagcg ggcgggacgg
cccttctcct ccgggctgta attagcgctt ggtttaatga 960 cggcttgttt
cttttctgtg gctgcgtgaa agccttgagg ggctccggga gggccctttg 1020
tgcgggggga gcggctcggg gctgtccgcg gggggacggc tgccttcggg ggggacgggg
1080 cagggcgggg ttcggcttct ggcgtgtgac cggcggctct agcagcctct
gctaaccatg 1140 ttcatgcctt cttctttttc ctacagctcc tgggcaacgt
gctggttatt gtgctgtctc 1200 atcattttgg caaagaatta agcttgagct
cgcgatccgc agccatggat tatggaggcg 1260 ccctgagtgc cgttgggcgc
gagctgctat ttgtaacgaa cccagtagtc gtcaatggct 1320 ctgtacttgt
gcctgaggac cagtgttact gcgcgggctg gattgagtcg cgtggcacaa 1380
acggtgccca aacggcgtcg aacgtgctgc aatggcttgc tgctggcttc tccatcctac
1440 tgcttatgtt ttacgcctac caaacatgga agtcaacctg cggctgggag
gagatctatg 1500 tgtgcgctat cgagatggtc aaggtgattc ttgagttctt
cttcgagttt aagaacccgt 1560 ccatgctgta tctagccaca ggccaccgcg
tccagtggtt gcgttacgcc gagtggcttc 1620 tcacctgccc ggtcattctc
attcacctgt caaacctgac gggcttgtcc aacgactaca 1680 gcaggcgcac
tatgggtctg cttgtgtctg atattggcac aattgtgtgg ggcgccactt 1740
ccgctatggc caccggatac gtcaaggtca tcttcttctg cctgggtctg tgttatggtg
1800 ctaacacgtt ctttcacgct gccaaggcct acatcgaggg ttaccatacc
gtgccgaagg 1860 gccggtgtcg ccaggtggtg actggcatgg cttggctctt
cttcgtatca tggggtatgt 1920 tccccatcct gttcatcctc ggccccgagg
gcttcggcgt cctgagcgtg tacggctcca 1980 ccgtcggcca caccatcatt
gacctgatgt cgaagaactg ctggggtctg ctcggccact 2040 acctgcgcgt
gctgatccac gagcatatcc tcatccacgg cgacattcgc aagaccacca 2100
aattgaacat tggtggcact gagattgagg tcgagacgct ggtggaggac gaggccgagg
2160 ctggcgcggt caacaagggc accggcaagg aattcggagg cggaggtgga
gctagcaaag 2220 gagaagaact cttcactgga gttgtcccaa ttcttgttga
attagatggt gatgttaacg 2280
gccacaagtt ctctgtcagt ggagagggtg aaggtgatgc aacatacgga aaacttaccc
2340 tgaagttcat ctgcactact ggcaaactgc ctgttccatg gccaacacta
gtcactactc 2400 tgtgctatgg tgttcaatgc ttttcaagat acccggatca
tatgaaacgg catgactttt 2460 tcaagagtgc catgcccgaa ggttatgtac
aggaaaggac catcttcttc aaagatgacg 2520 gcaactacaa gacacgtgct
gaagtcaagt ttgaaggtga tacccttgtt aatagaatcg 2580 agttaaaagg
tattgacttc aaggaagatg gcaacattct gggacacaaa ttggaataca 2640
actataactc acacaatgta tacatcatgg cagacaaaca aaagaatgga atcaaagtga
2700 acttcaagac ccgccacaac attgaagatg gaagcgttca actagcagac
cattatcaac 2760 aaaatactcc aattggcgat ggccctgtcc ttttaccaga
caaccattac ctgtccacac 2820 aatctgccct ttcgaaagat cccaacgaaa
agagagacca catggtcctt cttgagtttg 2880 taacagctgc tgggattaca
catggcatgg atgaactgta caacgtggtt cttcggaccg 2940 cctgtccccc
agattacaca ctagctatga ggaggtcacc tgatgatgtt cccttaatga 3000
cacccaacac cattacaatg taactcgagt ctagacgtgg taccgataat caacctctgg
3060 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
tttacgctat 3120 gtggatacgc tgctttaatg cctttgtatc atgctattgc
ttcccgtatg gctttcattt 3180 tctcctcctt gtataaatcc tggttgctgt
ctctttatga ggagttgtgg cccgttgtca 3240 ggcaacgtgg cgtggtgtgc
actgtgtttg ctgacgcaac ccccactggt tggggcattg 3300 ccaccacctg
tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg 3360
aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
3420 attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc
tgtgttgcca 3480 cctggattct gcgcgggacg tccttctgct acgtcccttc
ggccctcaat ccagcggacc 3540 ttccttcccg cggcctgctg ccggctctgc
ggcctcttcc gcgtcttcgc cttcgccctc 3600 agacgagtcg gatctccctt
tgggccgcct ccccgcctga tgcggggatc ctctagagtc 3660 gagagatcta
cgggtggcat ccctgtgacc cctccccagt gcctctcctg gccctggaag 3720
ttgccactcc agtgcccacc agccttgtcc taataaaatt aagttgcatc attttgtctg
3780 actaggtgtc cttctataat attatggggt ggaggggggt ggtatggagc
aaggggcaag 3840 ttgggaagac aacctgtagg gcctgcgggg tctattggga
accaagctgg agtgcagtgg 3900 cacaatcttg gctcactgca atctccgcct
cctgggttca agcgattctc ctgcctcagc 3960 ctcccgagtt gttgggattc
caggcatgca tgaccaggct cagctaattt ttgttttttt 4020 ggtagagacg
gggtttcacc atattggcca ggctggtctc caactcctaa tctcaggtga 4080
tctacccacc ttggcctccc aaattgctgg gattacaggc gtgaaccact gctcccttcc
4140 ctgtccttct gattttgtag gtaaccacgt gcggaccgag cggccgcagg
aacccctagt 4200 gatagagttg gccactccct ctctgcgcgc tcgctcgctc
actgaggccg ggcgaccaaa 4260 ggtcgcccga cgcccgggct ttgcccgggc
ggcctcagtg agcgagcgag cgcgcagctg 4320 cctgcagg 4328 <210> SEQ
ID NO 37 <211> LENGTH: 3620 <212> TYPE: DNA <213>
ORGANISM: Artificial Sequence <220> FEATURE: <223>
OTHER INFORMATION: 5'-
ITR-CAG-ChR2-{NLG-1Motif}-WPRE-bGHpolyA-ITR-3' <400>
SEQUENCE: 37 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag
cccgggcgtc 60 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc
gcgcagagag ggagtggcca 120 actccatcac taggggttcc tgcggccgca
cgcgtgatat cctagttatt aatagtaatc 180 aattacgggg tcattagttc
atagcccata tatggagttc cgcgttacat aacttacggt 240 aaatggcccg
cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta 300
tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
360 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
cccctattga 420 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
tacatgacct tatgggactt 480 tcctacttgg cagtacatct acgtattagt
catcgctatt accatgcatg gtcgaggtga 540 gccccacgtt ctgcttcact
ctccccatct cccccccctc cccaccccca attttgtatt 600 tatttatttt
ttaattattt tgtgcagcga tgggggcggg gggggggggg gggcgcgcgc 660
caggcggggc ggggcggggc gaggggcggg gcggggcgag gcggagaggt gcggcggcag
720 ccaatcagag cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg
cggcggcggc 780 cctataaaaa gcgaagcgcg cggcgggcgg gagtcgctgc
gacgctgcct tcgccccgtg 840 ccccgctccg ccgccgcctc gcgccgcccg
ccccggctct gactgaccgc gttactccca 900 caggtgagcg ggcgggacgg
cccttctcct ccgggctgta attagcgctt ggtttaatga 960 cggcttgttt
cttttctgtg gctgcgtgaa agccttgagg ggctccggga gggccctttg 1020
tgcgggggga gcggctcggg gctgtccgcg gggggacggc tgccttcggg ggggacgggg
1080 cagggcgggg ttcggcttct ggcgtgtgac cggcggctct agcagcctct
gctaaccatg 1140 ttcatgcctt cttctttttc ctacagctcc tgggcaacgt
gctggttatt gtgctgtctc 1200 atcattttgg caaagaatta agcttgagct
cgcgatccgc agccatggat tatggaggcg 1260 ccctgagtgc cgttgggcgc
gagctgctat ttgtaacgaa cccagtagtc gtcaatggct 1320 ctgtacttgt
gcctgaggac cagtgttact gcgcgggctg gattgagtcg cgtggcacaa 1380
acggtgccca aacggcgtcg aacgtgctgc aatggcttgc tgctggcttc tccatcctac
1440 tgcttatgtt ttacgcctac caaacatgga agtcaacctg cggctgggag
gagatctatg 1500 tgtgcgctat cgagatggtc aaggtgattc ttgagttctt
cttcgagttt aagaacccgt 1560 ccatgctgta tctagccaca ggccaccgcg
tccagtggtt gcgttacgcc gagtggcttc 1620 tcacctgccc ggtcattctc
attcacctgt caaacctgac gggcttgtcc aacgactaca 1680 gcaggcgcac
tatgggtctg cttgtgtctg atattggcac aattgtgtgg ggcgccactt 1740
ccgctatggc caccggatac gtcaaggtca tcttcttctg cctgggtctg tgttatggtg
1800 ctaacacgtt ctttcacgct gccaaggcct acatcgaggg ttaccatacc
gtgccgaagg 1860 gccggtgtcg ccaggtggtg actggcatgg cttggctctt
cttcgtatca tggggtatgt 1920 tccccatcct gttcatcctc ggccccgagg
gcttcggcgt cctgagcgtg tacggctcca 1980 ccgtcggcca caccatcatt
gacctgatgt cgaagaactg ctggggtctg ctcggccact 2040 acctgcgcgt
gctgatccac gagcatatcc tcatccacgg cgacattcgc aagaccacca 2100
aattgaacat tggtggcact gagattgagg tcgagacgct ggtggaggac gaggccgagg
2160 ctggcgcggt caacaagggc accggcaagg aattcggagg cggaggtgga
gctagcgtgg 2220 ttcttcggac cgcctgtccc ccagattaca cactagctat
gaggaggtca cctgatgatg 2280 ttcccttaat gacacccaac accattacaa
tgtaactcga gtctagacgt ggtaccgata 2340 atcaacctct ggattacaaa
atttgtgaaa gattgactgg tattcttaac tatgttgctc 2400 cttttacgct
atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta 2460
tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
2520 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
acccccactg 2580 gttggggcat tgccaccacc tgtcagctcc tttccgggac
tttcgctttc cccctcccta 2640 ttgccacggc ggaactcatc gccgcctgcc
ttgcccgctg ctggacaggg gctcggctgt 2700 tgggcactga caattccgtg
gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg 2760 cctgtgttgc
cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca 2820
atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
2880 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct
gatgcgggga 2940 tcctctagag tcgagagatc tacgggtggc atccctgtga
cccctcccca gtgcctctcc 3000 tggccctgga agttgccact ccagtgccca
ccagccttgt cctaataaaa ttaagttgca 3060 tcattttgtc tgactaggtg
tccttctata atattatggg gtggaggggg gtggtatgga 3120 gcaaggggca
agttgggaag acaacctgta gggcctgcgg ggtctattgg gaaccaagct 3180
ggagtgcagt ggcacaatct tggctcactg caatctccgc ctcctgggtt caagcgattc
3240 tcctgcctca gcctcccgag ttgttgggat tccaggcatg catgaccagg
ctcagctaat 3300 ttttgttttt ttggtagaga cggggtttca ccatattggc
caggctggtc tccaactcct 3360 aatctcaggt gatctaccca ccttggcctc
ccaaattgct gggattacag gcgtgaacca 3420 ctgctccctt ccctgtcctt
ctgattttgt aggtaaccac gtgcggaccg agcggccgca 3480 ggaaccccta
gtgatggagt tggccactcc ctctctgcgc gctcgctcgc tcactgaggc 3540
cgggcgacca aaggtcgccc gacgcccggg ctttgcccgg gcggcctcag tgagcgagcg
3600 agcgcgcagc tgcctgcagg 3620 <210> SEQ ID NO 38
<211> LENGTH: 4310 <212> TYPE: DNA <213>
ORGANISM: Artificial Sequence <220> FEATURE: <223>
OTHER INFORMATION: 5'-ITR-CAG-ChR2-GFP- {MLPH
Motif}-WPRE-bGHpolyA-ITR- 3' <400> SEQUENCE: 38 cctgcaggca
gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc 60
gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
120 actccatcac taggggttcc tgcggccgca cgcgtgatat cctagttatt
aatagtaatc 180 aattacgggg tcattagttc atagcccata tatggagttc
cgcgttacat aacttacggt 240 aaatggcccg cctggctgac cgcccaacga
cccccgccca ttgacgtcaa taatgacgta 300 tgttcccata gtaacgccaa
tagggacttt ccattgacgt caatgggtgg agtatttacg 360 gtaaactgcc
cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga 420
cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
480 tcctacttgg cagtacatct acgtattagt catcgctatt accatgcatg
gtcgaggtga 540 gccccacgtt ctgcttcact ctccccatct cccccccctc
cccaccccca attttgtatt 600 tatttatttt ttaattattt tgtgcagcga
tgggggcggg gggggggggg gggcgcgcgc 660 caggcggggc ggggcggggc
gaggggcggg gcggggcgag gcggagaggt gcggcggcag 720 ccaatcagag
cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg cggcggcggc 780
cctataaaaa gcgaagcgcg cggcgggcgg gagtcgctgc gacgctgcct tcgccccgtg
840 ccccgctccg ccgccgcctc gcgccgcccg ccccggctct gactgaccgc
gttactccca 900 caggtgagcg ggcgggacgg cccttctcct ccgggctgta
attagcgctt ggtttaatga 960 cggcttgttt cttttctgtg gctgcgtgaa
agccttgagg ggctccggga gggccctttg 1020 tgcgggggga gcggctcggg
gctgtccgcg gggggacggc tgccttcggg ggggacgggg 1080
cagggcgggg ttcggcttct ggcgtgtgac cggcggctct agcagcctct gctaaccatg
1140 ttcatgcctt cttctttttc ctacagctcc tgggcaacgt gctggttatt
gtgctgtctc 1200 atcattttgg caaagaatta agcttgagct cgcgatccgc
agccatggat tatggaggcg 1260 ccctgagtgc cgttgggcgc gagctgctat
ttgtaacgaa cccagtagtc gtcaatggct 1320 ctgtacttgt gcctgaggac
cagtgttact gcgcgggctg gattgagtcg cgtggcacaa 1380 acggtgccca
aacggcgtcg aacgtgctgc aatggcttgc tgctggcttc tccatcctac 1440
tgcttatgtt ttacgcctac caaacatgga agtcaacctg cggctgggag gagatctatg
1500 tgtgcgctat cgagatggtc aaggtgattc ttgagttctt cttcgagttt
aagaacccgt 1560 ccatgctgta tctagccaca ggccaccgcg tccagtggtt
gcgttacgcc gagtggcttc 1620 tcacctgccc ggtcattctc attcacctgt
caaacctgac gggcttgtcc aacgactaca 1680 gcaggcgcac tatgggtctg
cttgtgtctg atattggcac aattgtgtgg ggcgccactt 1740 ccgctatggc
caccggatac gtcaaggtca tcttcttctg cctgggtctg tgttatggtg 1800
ctaacacgtt ctttcacgct gccaaggcct acatcgaggg ttaccatacc gtgccgaagg
1860 gccggtgtcg ccaggtggtg actggcatgg cttggctctt cttcgtatca
tggggtatgt 1920 tccccatcct gttcatcctc ggccccgagg gcttcggcgt
cctgagcgtg tacggctcca 1980 ccgtcggcca caccatcatt gacctgatgt
cgaagaactg ctggggtctg ctcggccact 2040 acctgcgcgt gctgatccac
gagcatatcc tcatccacgg cgacattcgc aagaccacca 2100 aattgaacat
tggtggcact gagattgagg tcgagacgct ggtggaggac gaggccgagg 2160
ctggcgcggt caacaagggc accggcaagg aattcggagg cggaggtgga gctagcaaag
2220 gagaagaact cttcactgga gttgtcccaa ttcttgttga attagatggt
gatgttaacg 2280 gccacaagtt ctctgtcagt ggagagggtg aaggtgatgc
aacatacgga aaacttaccc 2340 tgaagttcat ctgcactact ggcaaactgc
ctgttccatg gccaacacta gtcactactc 2400 tgtgctatgg tgttcaatgc
ttttcaagat acccggatca tatgaaacgg catgactttt 2460 tcaagagtgc
catgcccgaa ggttatgtac aggaaaggac catcttcttc aaagatgacg 2520
gcaactacaa gacacgtgct gaagtcaagt ttgaaggtga tacccttgtt aatagaatcg
2580 agttaaaagg tattgacttc aaggaagatg gcaacattct gggacacaaa
ttggaataca 2640 actataactc acacaatgta tacatcatgg cagacaaaca
aaagaatgga atcaaagtga 2700 acttcaagac ccgccacaac attgaagatg
gaagcgttca actagcagac cattatcaac 2760 aaaatactcc aattggcgat
ggccctgtcc ttttaccaga caaccattac ctgtccacac 2820 aatctgccct
ttcgaaagat cccaacgaaa agagagacca catggtcctt cttgagtttg 2880
taacagctgc tgggattaca catggcatgg atgaactgta caacagggac cagcctctga
2940 acagcaaaaa gaaaaagagg ctcctgagct tcagggacgt ggacttcgag
gaggacagcg 3000 actaactcga gtctagacgt ggtaccgata atcaacctct
ggattacaaa atttgtgaaa 3060 gattgactgg tattcttaac tatgttgctc
cttttacgct atgtggatac gctgctttaa 3120 tgcctttgta tcatgctatt
gcttcccgta tggctttcat tttctcctcc ttgtataaat 3180 cctggttgct
gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt 3240
gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
3300 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
gccgcctgcc 3360 ttgcccgctg ctggacaggg gctcggctgt tgggcactga
caattccgtg gtgttgtcgg 3420 ggaagctgac gtcctttcca tggctgctcg
cctgtgttgc cacctggatt ctgcgcggga 3480 cgtccttctg ctacgtccct
tcggccctca atccagcgga ccttccttcc cgcggcctgc 3540 tgccggctct
gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc 3600
tttgggccgc ctccccgcct gatgcgggga tcctctagag tcgagagatc tacgggtggc
3660 atccctgtga cccctcccca gtgcctctcc tggccctgga agttgccact
ccagtgccca 3720 ccagccttgt cctaataaaa ttaagttgca tcattttgtc
tgactaggtg tccttctata 3780 atattatggg gtggaggggg gtggtatgga
gcaaggggca agttgggaag acaacctgta 3840 gggcctgcgg ggtctattgg
gaaccaagct ggagtgcagt ggcacaatct tggctcactg 3900 caatctccgc
ctcctgggtt caagcgattc tcctgcctca gcctcccgag ttgttgggat 3960
tccaggcatg catgaccagg ctcagctaat ttttgttttt ttggtagaga cggggtttca
4020 ccatattggc caggctggtc tccaactcct aatctcaggt gatctaccca
ccttggcctc 4080 ccaaattgct gggattacag gcgtgaacca ctgctccctt
ccctgtcctt ctgattttgt 4140 aggtaaccac gtgcggaccg agcggccgca
ggaaccccta gtgatggagt tggccactcc 4200 ctctctgcgc gctcgctcgc
tcactgaggc cgggcgacca aaggtcgccc gacgcccggg 4260 ctttgcccgg
gcggcctcag tgagcgagcg agcgcgcagc tgcctgcagg 4310 <210> SEQ ID
NO 39 <211> LENGTH: 3602 <212> TYPE: DNA <213>
ORGANISM: Artificial Sequence <220> FEATURE: <223>
OTHER INFORMATION: 5'-
ITR-CAG-ChR2-{MLPH-Motif}-WPRE-bGHpolyA-ITR-3' <400>
SEQUENCE: 39 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag
cccgggcgtc 60 gggcgacctt tggtcgcccg gccgcagtga gcgagcgagc
gcgcagagag ggagtggcca 120 actccatcac taggggttcc tgcggccgca
cgcgtgatat cctagttatt aatagtaatc 180 aattacgggg tcattagttc
atagcccata tatggagttc cgcgttacat aacttacggt 240 aaatggcccg
cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta 300
tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
360 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
cccctattga 420 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
tacatgacct tatgggactt 480 tcctacttgg cagtacatct acgtattagt
catcgctatt accatgcatg gtcgaggtga 540 gccccacgtt ctgcttcact
ctccccatct cccccccctc cccaccccca attttgtatt 600 tatttatttt
ttaattattt tgtgcagcga tgggggcggg gggggggggg gggcgcgcgc 660
caggcggggc ggggcggggc gaggggcggg gcggggcgag gcggagaggt gcggcggcag
720 ccaatcagag cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg
cggcggcggc 780 cctataaaaa gcgaagcgcg cggcgggcgg gagtcgctgc
gacgctgcct tcgccccgtg 840 ccccgctccg ccgccgcctc gcgccgcccg
ccccggctct gactgaccgc gttactccca 900 caggtgagcg ggcgggacgg
cccttctcct ccgggctgta attagcgctt ggtttaatga 960 cggcttgttt
cttttctgtg gctgcgtgaa agccttgagg ggctccggga gggccctttg 1020
tgcgggggga gcggctcggg gctgtccgcg gggggacggc tgccttcggg ggggacgggg
1080 cagggcgggg ttcggcttct ggcgtgtgac cggcggctct agcagcctct
gctaaccatg 1140 ttcatgcctt cttctttttc ctacagctcc tgggcaacgt
gctggttatt gtgctgtctc 1200 atcattttgg caaagaatta agcttgagct
cgcgatccgc agccatggat tatggaggcg 1260 ccctgagtgc cgttgggcgc
gagctgctat ttgtaacgaa cccagtagtc gtcaatggct 1320 ctgtacttgt
gcctgaggac cagtgttact gcgcgggctg gattgagtcg cgtggcacaa 1380
acggtgccca aacggcgtcg aacgtgctgc aatggcttgc tgctggcttc tccatcctac
1440 tgcttatgtt ttacgcctac caaacatgga agtcaacctg cggctgggag
gagatctatg 1500 tgtgcgctat cgagatggtc aaggtgattc ttgagttctt
cttcgagttt aagaacccgt 1560 ccatgctgta tctagccaca ggccaccgcg
tccagtggtt gcgttacgcc gagtggcttc 1620 tcacctgccc ggtcattctc
attcacctgt caaacctgac gggcttgtcc aacgactaca 1680 gcaggcgcac
tatgggtctg cttgtgtctg atattggcac aattgtgtgg ggcgccactt 1740
ccgctatggc caccggatac gtcaaggtca tcttcttctg cctgggtctg tgttatggtg
1800 ctaacacgtt ctttcacgct gccaaggcct acatcgaggg ttaccatacc
gtgccgaagg 1860 gccggtgtcg ccaggtggtg actggcatgg cttggctctt
cttcgtatca tggggtatgt 1920 tccccatcct gttcatcctc ggccccgagg
gcttcggcgt cctgagcgtg tacggctcca 1980 ccgtcggcca caccatcatt
gacctgatgt cgaagaactg ctggggtctg ctcggccact 2040 acctgcgcgt
gctgatccac gagcatatcc tcatccacgg cgacattcgc aagaccacca 2100
aattgaacat tggtggcact gagattgagg tcgagacgct ggtggaggac gaggccgagg
2160 ctggcgcggt caacaagggc accggcaagg aattcggagg cggaggtgga
gctagcaggg 2220 accagcctct gaacagcaaa aagaaaaaga ggctcctgag
cttcagggac gtggacttcg 2280 aggaggacag cgactaactc gagtctagac
gtggtaccga taatcaacct ctggattaca 2340 aaatttgtga aagattgact
ggtattctta actatgttgc tccttttacg ctatgtggat 2400 acgctgcttt
aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct 2460
ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
2520 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
attgccacca 2580 cctgtcagct cctttccggg actttcgctt tccccctccc
tattgccacg gcggaactca 2640 tcgccgcctg ccttgcccgc tgctggacag
gggctcggct gttgggcact gacaattccg 2700 tggtgttgtc ggggaagctg
acgtcctttc catggctgct cgcctgtgtt gccacctgga 2760 ttctgcgcgg
gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt 2820
cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga
2880 gtcggatctc cctttgggcc gcctccccgc ctgatgcggg gatcctctag
agtcgagaga 2940 tctacgggtg gcatccctgt gacccctccc cagtgcctct
cctggccctg gaagttgcca 3000 ctccagtgcc caccagcctt gtcctaataa
aattaagttg catcattttg tctgactagg 3060 tgtccttcta taatattatg
gggtggaggg gggtggtatg gagcaagggg caagttggga 3120 agacaacctg
tagggcctgc ggggtctatt gggaaccaag ctggagtgca gtggcacaat 3180
cttggctcac tgcaatctcc gcctcctggg ttcaagcgat tctcctgcct cagcctcccg
3240 agttgttggg attccaggca tgcatgacca ggctcagcta atttttgttt
ttttggtaga 3300 gacggggttt caccatattg gccaggctgg tctccaactc
ctaatctcag gtgatctacc 3360 caccttggcc tcccaaattg ctgggattac
aggcgtgaac cactgctccc ttccctgtcc 3420 ttctgatttt gtaggtaacc
acgtgcggac cgagcggccg caggaacccc tagtgatgga 3480 gttggccact
ccctctctgc gcgctcgctc gctcactgag gccgggcgac caaaggtcgc 3540
ccgacgcccg ggctttgccc gggcggcctc agtgagcgag cgagcgcgca gctgcctgca
3600 gg 3602 <210> SEQ ID NO 40 <211> LENGTH: 4361
<212> TYPE: DNA <213> ORGANISM: Artificial Sequence
<220> FEATURE: <223> OTHER INFORMATION: 5'-
ITR-CAG-HaloR-GFP-{Kv2 .lMotif}- WPRE-bGHpolyA-ITR-3' <400>
SEQUENCE: 40
cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
60 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag
ggagtggcca 120 actccatcac taggggttcc tgcggccgca cgcgtgatat
cctagttatt aatagtaatc 180 aattacgggg tcattagttc atagcccata
tatggagttc cgcgttacat aacttacggt 240 aaatggcccg cctggctgac
cgcccaacga cccccgccca ttgacgtcaa taatgacgta 300 tgttcccata
gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg 360
gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
420 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
tatgggactt 480 tcctacttgg cagtacatct acgtattagt catcgctatt
accatgcatg gtcgaggtga 540 gccccacgtt ctgcttcact ctccccatct
cccccccctc cccaccccca attttgtatt 600 tatttatttt ttaattattt
tgtgcagcga tgggggcggg gggggggggg gggcgcgcgc 660 caggcggggc
ggggcggggc gaggggcggg gcggggcgag gcggagaggt gcggcggcag 720
ccaatcagag cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg cggcggcggc
780 cctataaaaa gcgaagcgcg cggcgggcgg gagtcgctgc gacgctgcct
tcgccccgtg 840 ccccgctccg ccgccgcctc gcgccgcccg ccccggctct
gactgaccgc gttactccca 900 caggtgagcg ggcgggacgg cccttctcct
ccgggctgta attagcgctt ggtttaatga 960 cggcttgttt cttttctgtg
gctgcgtgaa agccttgagg ggctccggga gggccctttg 1020 tgcgggggga
gcggctcggg gctgtccgcg gggggacggc tgccttcggg ggggacgggg 1080
cagggcgggg ttcggcttct ggcgtgtgac cggcggctct agcagcctct gctaaccatg
1140 ttcatgcctt cttctttttc ctacagctcc tgggcaacgt gctggttatt
gtgctgtctc 1200 atcattttgg caaagaatta agcttgagct cgcgatccgc
agccatgact gagacattgc 1260 caccggtaac ggaatcggct gttgcgctac
aggcggaggt gacccagagg gagctgttcg 1320 agttcgttct caacgacccc
ctcctcgcca gttcgctgta tattaatatc gcactggcag 1380 ggctgtcgat
actgcttttc gtgttcatga cgcgcggact cgacgaccca cgggcgaaac 1440
tcatcgccgt ttcgacgatt ttggtgccgg tggtctctat cgcgagctac accggccttg
1500 catcggggct caccatcagc gtcctcgaga tgccagccgg ccacttcgcc
gaggggtcct 1560 cggtgatgct cggcggcgaa gaggtagacg gcgtcgtgac
gatgtggggc cgctatctga 1620 cgtgggccct ttcgacaccg atgatactgc
tggcgcttgg gctgcttgct ggctctaacg 1680 ccacgaagct ctttaccgcc
atcaccttcg acatcgcgat gtgtgtcacc ggcctcgcag 1740 ccgcgctgac
gacctcttcg cacctgatgc ggtggttctg gtacgccatc agttgtgcgt 1800
gtttcctcgt cgtcctctac atcctgctcg tcgagtgggc acaggacgcc aaggctgccg
1860 gtactgcgga tatgttcaat acgctgaagc tgctgaccgt tgtcatgtgg
ctcggctacc 1920 ccatcgtgtg ggcactcggc gttgagggca tcgccgttct
tccggtcgga gtcacgtcgt 1980 ggggatacag cttcctcgac atcgtcgcga
agtacatctt cgcgttcctg ctgctcaact 2040 acctcacgtc gaacgagagc
gtcgtctccg gctcgatact cgacgtgccg tccgcgtcgg 2100 gcactcccgc
tgacgacgaa ttcggaggcg gaggtggagc tagcaaagga gaagaactct 2160
tcactggagt tggagttgtc ccaattcttg ttgaattaga tggtgatgtt aacggccaca
2220 agttctctgt cagtggagag ggtgaaggtg atgcaacata cggaaaactt
accctgaagt 2280 tcatctgcac tactggcaaa ctgcctgttc catggccaac
actagtcact actctgtgct 2340 atggtgttca atgcttttca agatacccgg
atcatatgaa acggcatgac tttttcaaga 2400 gtgccatgcc cgaaggttat
gtacaggaaa ggaccatctt cttcaaagat gacggcaact 2460 acaagacacg
tgctgaagtc aagtttgaag gtgataccct tgttaataga atcgagttaa 2520
aaggtattga cttcaaggaa gatggcaaca ttctgggaca caaattggaa tacaactata
2580 actcacacaa tgtatacatc atggcagaca aacaaaagaa tggaatcaaa
gtgaacttca 2640 agacccgcca caacattgaa gatggaagcg ttcaactagc
agaccattat caacaaaata 2700 ctccaattgg cgatggccct gtccttttac
cagacaacca ttacctgtcc acacaatctg 2760 ccctttcgaa agatcccaac
gaaaagagag accacatggt ccttcttgag tttgtaacag 2820 ctgctgggat
tacacatggc atggatgaac tgtacaacca gtctcagccc atcctgaaca 2880
ctaaggagat ggcccctcag agtaaacccc ctgaggaact ggaaatgagc tccatgccat
2940 ctccagtggc tcctctgcca gctaggaccg agggcgtgat tgacatgaga
agcatgtcta 3000 gtatcgatag cttcatttcc tgcgccaccg acttccccga
agctacaagg ttttaactcg 3060 agtctagacg tggtaccgat aatcaacctc
tggattacaa aatttgtgaa agattgactg 3120 gtattcttaa ctatgttgct
ccttttacgc tatgtggata cgctgcttta atgcctttgt 3180 atcatgctat
tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc 3240
tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
3300 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
ctttccggga 3360 ctttcgcttt ccccctccct attgccacgg cggaactcat
cgccgcctgc cttgcccgct 3420 gctggacagg ggctcggctg ttgggcactg
acaattccgt ggtgttgtcg gggaagctga 3480 cgtcctttcc atggctgctc
gcctgtgttg ccacctggat tctgcgcggg acgtccttct 3540 gctacgtccc
ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc 3600
tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
3660 cctccccgcc tgatgcgggg atcctctaga gtcgagagat ctacgggtgg
catccctgtg 3720 acccctcccc agtgcctctc ctggccctgg aagttgccac
tccagtgccc accagccttg 3780 tcctaataaa attaagttgc atcattttgt
ctgactaggt gtccttctat aatattatgg 3840 ggtggagggg ggtggtatgg
agcaaggggc aagttgggaa gacaacctgt agggcctgcg 3900 gggtctattg
ggaaccaagc tggagtgcag tggcacaatc ttggctcact gcaatctccg 3960
cctcctgggt tcaagcgatt ctcctgcctc agcctcccga gttgttggga ttccaggcat
4020 gcatgaccag gctcagctaa tttttgtttt tttggtagag acggggtttc
accatattgg 4080 ccaggctggt ctccaactcc taatctcagg tgatctaccc
accttggcct cccaaattgc 4140 tgggattaca ggcgtgaacc actgctccct
tccctgtcct tctgattttg taggtaacca 4200 cgtgcggacc gagcggccgc
aggaacccct agtgatggag ttggccactc cctctctgcg 4260 cgctcgctcg
ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg 4320
ggcggcctca gtgagcgagc gagcgcgcag ctgcctgcag g 4361 <210> SEQ
ID NO 41 <211> LENGTH: 3647 <212> TYPE: DNA <213>
ORGANISM: Artificial Sequence <220> FEATURE: <223>
OTHER INFORMATION: 5' -
ITR-CAG-HaloR-{Kv2.1Motif}-WPRE-bGHpolyA-ITR- 3' <400>
SEQUENCE: 41 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag
cccgggcgtc 60 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc
gcgcagagag ggagtggcca 120 actccatcac taggggttcc tgcggccgca
cgcgtgatat cctagttatt aatagtaatc 180 aattacgggg tcattagttc
atagcccata tatggagttc cgcgttacat aacttacggt 240 aaatggcccg
cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta 300
tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
360 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
cccctattga 420 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
tacatgacct tatgggactt 480 tcctacttgg cagtacatct acgtattagt
catcgctatt accatgcatg gtcgaggtga 540 gccccacgtt ctgcttcact
ctccccatct cccccccctc cccaccccca attttgtatt 600 tatttatttt
ttaattattt tgtgcagcga tgggggcggg gggggggggg gggcgcgcgc 660
caggcggggc ggggcggggc gaggggcggg gcggggcgag gcggagaggt gcggcggcag
720 ccaatcagag cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg
cggcggcggc 780 cctataaaaa gcgaagcgcg cggcgggcgg gagtcgctgc
gacgctgcct tcgccccgtg 840 ccccgctccg ccgccgcctc gcgccgcccg
ccccggctct gactgaccgc gttactccca 900 caggtgagcg ggcgggacgg
cccttctcct ccgggctgta attagcgctt ggtttaatga 960 cggcttgttt
cttttctgtg gctgcgtgaa agccttgagg ggctccggga gggccctttg 1020
tgcgggggga gcggctcggg gctgtccgcg gggggacggc tgccttcggg ggggacgggg
1080 cagggcgggg ttcggcttct ggcgtgtgac cggcggctct agcagcctct
gctaaccatg 1140 ttcatgcctt cttctttttc ctacagctcc tgggcaacgt
gctggttatt gtgctgtctc 1200 atcattttgg caaagaatta agcttgagct
cgcgatccgc agccatgact gagacattgc 1260 caccggtaac ggaatcggct
gttgcgctac aggcggaggt gacccagagg gagctgttcg 1320 agttcgttct
caacgacccc ctcctcgcca gttcgctgta tattaatatc gcactggcag 1380
ggctgtcgat actgcttttc gtgttcatga cgcgcggact cgacgaccca cgggcgaaac
1440 tcatcgccgt ttcgacgatt ttggtgccgg tggtctctat cgcgagctac
accggccttg 1500 catcggggct caccatcagc gtcctcgaga tgccagccgg
ccacttcgcc gaggggtcct 1560 cggtgatgct cggcggcgaa gaggtagacg
gcgtcgtgac gatgtggggc cgctatctga 1620 cgtgggccct ttcgacaccg
atgatactgc tggcgcttgg gctgcttgct ggctctaacg 1680 ccacgaagct
ctttaccgcc atcaccttcg acatcgcgat gtgtgtcacc ggcctcgcag 1740
ccgcgctgac gacctcttcg cacctgatgc ggtggttctg gtacgccatc agttgtgcgt
1800 gtttcctcgt cgtcctctac atcctgctcg tcgagtgggc acaggacgcc
aaggctgccg 1860 gtactgcgga tatgttcaat acgctgaagc tgctgaccgt
tgtcatgtgg ctcggctacc 1920 ccatcgtgtg ggcactcggc gttgagggca
tcgccgttct tccggtcgga gtcacgtcgt 1980 ggggatacag cttcctcgac
atcgtcgcga agtacatctt cgcgttcctg ctgctcaact 2040 acctcacgtc
gaacgagagc gtcgtctccg gctcgatact cgacgtgccg tccgcgtcgg 2100
gcactcccgc tgacgacgaa ttcggaggcg gaggtggagc tagccagtct cagcccatcc
2160 tgaacactaa ggagatggcc cctcagagta aaccccctga ggaactggaa
atgagctcca 2220 tgccatctcc agtggctcct ctgccagcta ggaccgaggg
cgtgattgac atgagaagca 2280 tgtctagtat cgatagcttc atttcctgcg
ccaccgactt ccccgaagct acaaggtttt 2340 aactcgagtc tagacgtggt
accgataatc aacctctgga ttacaaaatt tgtgaaagat 2400 tgactggtat
tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc 2460
ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
2520 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
gtggtgtgca 2580 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
caccacctgt cagctccttt 2640 ccgggacttt cgctttcccc ctccctattg
ccacggcgga actcatcgcc gcctgccttg 2700 cccgctgctg gacaggggct
cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga 2760 agctgacgtc
ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt 2820
ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
2880 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
atctcccttt 2940 gggccgcctc cccgcctgat gcggggatcc tctagagtcg
agagatctac gggtggcatc 3000 cctgtgaccc ctccccagtg cctctcctgg
ccctggaagt tgccactcca gtgcccacca 3060 gccttgtcct aataaaatta
agttgcatca ttttgtctga ctaggtgtcc ttctataata 3120 ttatggggtg
gaggggggtg gtatggagca aggggcaagt tgggaagaca acctgtaggg 3180
cctgcggggt ctattgggaa ccaagctgga gtgcagtggc acaatcttgg ctcactgcaa
3240 tctccgcctc ctgggttcaa gcgattctcc tgcctcagcc tcccgagttg
ttgggattcc 3300 aggcatgcat gaccaggctc agctaatttt tgtttttttg
gtagagacgg ggtttcacca 3360 tattggccag gctggtctcc aactcctaat
ctcaggtgat ctacccacct tggcctccca 3420 aattgctggg attacaggcg
tgaaccactg ctcccttccc tgtccttctg attttgtagg 3480 taaccacgtg
cggaccgagc ggccgcagga acccctagtg atggagttgg ccactccctc 3540
tctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac gcccgggctt
3600 tgcccgggcg gcctcagtga gcgagcgagc gcgcagctgc ctgcagg 3647
<210> SEQ ID NO 42 <211> LENGTH: 4247 <212> TYPE:
DNA <213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: 5' - ITR-CAG-HaloR-GFP-{Nav1.6
Motif}-WPRE-bGHpolyA-ITR- 3' <400> SEQUENCE: 42 cctgcaggca
gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc 60
gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
120 actccatcac taggggttcc tgcggccgca cgcgtgatat cctagttatt
aatagtaatc 180 aattacgggg tcattagttc atagcccata tatggagttc
cgcgttacat aacttacggt 240 aaatggcccg cctggctgac cgcccaacga
cccccgccca ttgacgtcaa taatgacgta 300 tgttcccata gtaacgccaa
tagggacttt ccattgacgt caatgggtgg agtatttacg 360 gtaaactgcc
cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga 420
cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
480 tcctacttgg cagtacatct acgtattagt catcgctatt accatgcatg
gtcgaggtga 540 gccccacgtt ctgcttcact ctccccatct cccccccctc
cccaccccca attttgtatt 600 tatttatttt ttaattattt tgtgcagcga
tgggggcggg gggggggggg gggcgcgcgc 660 caggcggggc ggggcggggc
gaggggcggg gcggggcgag gcggagaggt gcggcggcag 720 ccaatcagag
cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg cggcggcggc 780
cctataaaaa gcgaagcgcg cggcgggcgg gagtcgctgc gacgctgcct tcgccccgtg
840 ccccgctccg ccgccgcctc gcgccgcccg ccccggctct gactgaccgc
gttactccca 900 caggtgagcg ggcgggacgg cccttctcct ccgggctgta
attagcgctt ggtttaatga 960 cggcttgttt cttttctgtg gctgcgtgaa
agccttgagg ggctccggga gggccctttg 1020 tgcgggggga gcggctcggg
gctgtccgcg gggggacggc tgccttcggg ggggacgggg 1080 cagggcgggg
ttcggcttct ggcgtgtgac cggcggctct agcagcctct gctaaccatg 1140
ttcatgcctt cttctttttc ctacagctcc tgggcaacgt gctggttatt gtgctgtctc
1200 atcattttgg caaagaatta agcttgagct cgcgatccgc agccatgact
gagacattgc 1260 caccggtaac ggaatcggct gttgcgctac aggcggaggt
gacccagagg gagctgttcg 1320 agttcgttct caacgacccc ctcctcgcca
gttcgctgta tattaatatc gcactggcag 1380 ggctgtcgat actgcttttc
gtgttcatga cgcgcggact cgacgaccca cgggcgaaac 1440 tcatcgccgt
ttcgacgatt ttggtgccgg tggtctctat cgcgagctac accggccttg 1500
catcggggct caccatcagc gtcctcgaga tgccagccgg ccacttcgcc gaggggtcct
1560 cggtgatgct cggcggcgaa gaggtagacg gcgtcgtgac gatgtggggc
cgctatctga 1620 cgtgggccct ttcgacaccg atgatactgc tggcgcttgg
gctgcttgct ggctctaacg 1680 ccacgaagct ctttaccgcc atcaccttcg
acatcgcgat gtgtgtcacc ggcctcgcag 1740 ccgcgctgac gacctcttcg
cacctgatgc ggtggttctg gtacgccatc agttgtgcgt 1800 gtttcctcgt
cgtcctctac atcctgctcg tcgagtgggc acaggacgcc aaggctgccg 1860
gtactgcgga tatgttcaat acgctgaagc tgctgaccgt tgtcatgtgg ctcggctacc
1920 ccatcgtgtg ggcactcggc gttgagggca tcgccgttct tccggtcgga
gtcacgtcgt 1980 ggggatacag cttcctcgac atcgtcgcga agtacatctt
cgcgttcctg ctgctcaact 2040 acctcacgtc gaacgagagc gtcgtctccg
gctcgatact cgacgtgccg tccgcgtcgg 2100 gcactcccgc tgacgacgaa
ttcggaggcg gaggtggagc tagcaaagga gaagaactct 2160 tcactggagt
tggagttgtc ccaattcttg ttgaattaga tggtgatgtt aacggccaca 2220
agttctctgt cagtggagag ggtgaaggtg atgcaacata cggaaaactt accctgaagt
2280 tcatctgcac tactggcaaa ctgcctgttc catggccaac actagtcact
actctgtgct 2340 atggtgttca atgcttttca agatacccgg atcatatgaa
acggcatgac tttttcaaga 2400 gtgccatgcc cgaaggttat gtacaggaaa
ggaccatctt cttcaaagat gacggcaact 2460 acaagacacg tgctgaagtc
aagtttgaag gtgataccct tgttaataga atcgagttaa 2520 aaggtattga
cttcaaggaa gatggcaaca ttctgggaca caaattggaa tacaactata 2580
actcacacaa tgtatacatc atggcagaca aacaaaagaa tggaatcaaa gtgaacttca
2640 agacccgcca caacattgaa gatggaagcg ttcaactagc agaccattat
caacaaaata 2700 ctccaattgg cgatggccct gtccttttac cagacaacca
ttacctgtcc acacaatctg 2760 ccctttcgaa agatcccaac gaaaagagag
accacatggt ccttcttgag tttgtaacag 2820 ctgctgggat tacacatggc
atggatgaac tgtacaacac cgtgagggtg cccatcgccg 2880 tgggcgagag
cgacttcgag aacctgaaca ccgaggacgt gagcagcgag agcgacccct 2940
aactcgagtc tagacgtggt accgataatc aacctctgga ttacaaaatt tgtgaaagat
3000 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
gctttaatgc 3060 ctttgtatca tgctattgct tcccgtatgg ctttcatttt
ctcctccttg tataaatcct 3120 ggttgctgtc tctttatgag gagttgtggc
ccgttgtcag gcaacgtggc gtggtgtgca 3180 ctgtgtttgc tgacgcaacc
cccactggtt ggggcattgc caccacctgt cagctccttt 3240 ccgggacttt
cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg 3300
cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
3360 agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg
cgcgggacgt 3420 ccttctgcta cgtcccttcg gccctcaatc cagcggacct
tccttcccgc ggcctgctgc 3480 cggctctgcg gcctcttccg cgtcttcgcc
ttcgccctca gacgagtcgg atctcccttt 3540 gggccgcctc cccgcctgat
gcggggatcc tctagagtcg agagatctac gggtggcatc 3600 cctgtgaccc
ctccccagtg cctctcctgg ccctggaagt tgccactcca gtgcccacca 3660
gccttgtcct aataaaatta agttgcatca ttttgtctga ctaggtgtcc ttctataata
3720 ttatggggtg gaggggggtg gtatggagca aggggcaagt tgggaagaca
acctgtaggg 3780 cctgcggggt ctattgggaa ccaagctgga gtgcagtggc
acaatcttgg ctcactgcaa 3840 tctccgcctc ctgggttcaa gcgattctcc
tgcctcagcc tcccgagttg ttgggattcc 3900 aggcatgcat gaccaggctc
agctaatttt tgtttttttg gtagagacgg ggtttcacca 3960 tattggccag
gctggtctcc aactcctaat ctcaggtgat ctacccacct tggcctccca 4020
aattgctggg attacaggcg tgaaccactg ctcccttccc tgtccttctg attttgtagg
4080 taaccacgtg cggaccgagc ggccgcagga acccctagtg atggagttgg
ccactccctc 4140 tctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag
gtcgcccgac gcccgggctt 4200 tgcccgggcg gccgcagtga gcgagcgagc
gcgcagctgc ctgcagg 4247 <210> SEQ ID NO 43 <211>
LENGTH: 3533 <212> TYPE: DNA <213> ORGANISM: Artificial
Sequence <220> FEATURE: <223> OTHER INFORMATION: 5'-
ITR-CAG-HaloR-{Nav1.6 Motif}-WPRE-bGHpolyA-ITR- 3' <400>
SEQUENCE: 43 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag
cccgggcgtc 60 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc
gcgcagagag ggagtggcca 120 actccatcac taggggttcc tgcggccgca
cgcgtgatat cctagttatt aatagtaatc 180 aattacgggg tcattagttc
atagcccata tatggagttc cgcgttacat aacttacggt 240 aaatggcccg
cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta 300
tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
360 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
cccctattga 420 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
tacatgacct tatgggactt 480 tcctacttgg cagtacatct acgtattagt
catcgctatt accatgcatg gtcgaggtga 540 gccccacgtt ctgcttcact
ctccccatct cccccccctc cccaccccca attttgtatt 600 tatttatttt
ttaattattt tgtgcagcga tgggggcggg gggggggggg gggcgcgcgc 660
caggcggggc ggggcggggc gaggggcggg gcggggcgag gcggagaggt gcggcggcag
720 ccaatcagag cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg
cggcggcggc 780 cctataaaaa gcgaagcgcg cggcgggcgg gagtcgctgc
gacgctgcct tcgccccgtg 840 ccccgctccg ccgccgcctc gcgccgcccg
ccccggctct gactgaccgc gttactccca 900 caggtgagcg ggcgggacgg
cccttctcct ccgggctgta attagcgctt ggtttaatga 960 cggcttgttt
cttttctgtg gctgcgtgaa agccttgagg ggctccggga gggccctttg 1020
tgcgggggga gcggctcggg gctgtccgcg gggggacggc tgccttcggg ggggacgggg
1080 cagggcgggg ttcggcttct ggcgtgtgac cggcggctct agcagcctct
gctaaccatg 1140 ttcatgcctt cttctttttc ctacagctcc tgggcaacgt
gctggttatt gtgctgtctc 1200 atcattttgg caaagaatta agcttgagct
cgcgatccgc agccatgact gagacattgc 1260 caccggtaac ggaatcggct
gttgcgctac aggcggaggt gacccagagg gagctgttcg 1320 agttcgttct
caacgacccc ctcctcgcca gttcgctgta tattaatatc gcactggcag 1380
ggctgtcgat actgcttttc gtgttcatga cgcgcggact cgacgaccca cgggcgaaac
1440 tcatcgccgt ttcgacgatt ttggtgccgg tggtctctat cgcgagctac
accggccttg 1500 catcggggct caccatcagc gtcctcgaga tgccagccgg
ccacttcgcc gaggggtcct 1560 cggtgatgct cggcggcgaa gaggtagacg
gcgtcgtgac gatgtggggc cgctatctga 1620 cgtgggccct ttcgacaccg
atgatactgc tggcgcttgg gctgcttgct ggctctaacg 1680 ccacgaagct
ctttaccgcc atcaccttcg acatcgcgat gtgtgtcacc ggcctcgcag 1740
ccgcgctgac gacctcttcg cacctgatgc ggtggttctg gtacgccatc agttgtgcgt
1800 gtttcctcgt cgtcctctac atcctgctcg tcgagtgggc acaggacgcc
aaggctgccg 1860 gtactgcgga tatgttcaat acgctgaagc tgctgaccgt
tgtcatgtgg ctcggctacc 1920 ccatcgtgtg ggcactcggc gttgagggca
tcgccgttct tccggtcgga gtcacgtcgt 1980 ggggatacag cttcctcgac
atcgtcgcga agtacatctt cgcgttcctg ctgctcaact 2040 acctcacgtc
gaacgagagc gtcgtctccg gctcgatact cgacgtgccg tccgcgtcgg 2100
gcactcccgc tgacgacgaa ttcggaggcg gaggtggagc tagcaccgtg agggtgccca
2160 tcgccgtggg cgagagcgac ttcgagaacc tgaacaccga ggacgtgagc
agcgagagcg 2220 acccctaact cgagtctaga cgtggtaccg ataatcaacc
tctggattac aaaatttgtg 2280 aaagattgac tggtattctt aactatgttg
ctccttttac gctatgtgga tacgctgctt 2340 taatgccttt gtatcatgct
attgcttccc gtatggcttt cattttctcc tccttgtata 2400 aatcctggtt
gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg 2460
tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
2520 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
atcgccgcct 2580 gccttgcccg ctgctggaca ggggctcggc tgttgggcac
tgacaattcc gtggtgttgt 2640 cggggaagct gacgtccttt ccatggctgc
tcgcctgtgt tgccacctgg attctgcgcg 2700 ggacgtcctt ctgctacgtc
ccttcggccc tcaatccagc ggaccttcct tcccgcggcc 2760 tgctgccggc
tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct 2820
ccctttgggc cgcctccccg cctgatgcgg ggatcctcta gagtcgagag atctacgggt
2880 ggcatccctg tgacccctcc ccagtgcctc tcctggccct ggaagttgcc
actccagtgc 2940 ccaccagcct tgtcctaata aaattaagtt gcatcatttt
gtctgactag gtgtccttct 3000 ataatattat ggggtggagg ggggtggtat
ggagcaaggg gcaagttggg aagacaacct 3060 gtagggcctg cggggtctat
tgggaaccaa gctggagtgc agtggcacaa tcttggctca 3120 ctgcaatctc
cgcctcctgg gttcaagcga ttctcctgcc tcagcctccc gagttgttgg 3180
gattccaggc atgcatgacc aggctcagct aatttttgtt tttttggtag agacggggtt
3240 tcaccatatt ggccaggctg gtctccaact cctaatctca ggtgatctac
ccaccttggc 3300 ctcccaaatt gctgggatta caggcgtgaa ccactgctcc
cttccctgtc cttctgattt 3360 tgtaggtaac cacgtgcgga ccgagcggcc
gcaggaaccc ctagtgatgg agttggccac 3420 tccctctctg cgcgctcgct
cgctcactga ggccgggcga ccaaaggtcg cccgacgccc 3480 gggctttgcc
cgggcggcct cagtgagcga gcgagcgcgc agctgcctgc agg 3533 <210>
SEQ ID NO 44 <211> LENGTH: 4262 <212> TYPE: DNA
<213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: 5' -ITR-CAG-HaloR-GFP- {NLG-1
Motif}-WPRE-bGHpolyA-ITR- 3' <400> SEQUENCE: 44 cctgcaggca
gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc 60
gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
120 actccatcac taggggttcc tgcggccgca cgcgtgatat cctagttatt
aatagtaatc 180 aattacgggg tcattagttc atagcccata tatggagttc
cgcgttacat aacttacggt 240 aaatggcccg cctggctgac cgcccaacga
cccccgccca ttgacgtcaa taatgacgta 300 tgttcccata gtaacgccaa
tagggacttt ccattgacgt caatgggtgg agtatttacg 360 gtaaactgcc
cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga 420
cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
480 tcctacttgg cagtacatct acgtattagt catcgctatt accatgcatg
gtcgaggtga 540 gccccacgtt ctgcttcact ctccccatct cccccccctc
cccaccccca attttgtatt 600 tatttatttt ttaattattt tgtgcagcga
tgggggcggg gggggggggg gggcgcgcgc 660 caggcggggc ggggcggggc
gaggggcggg gcggggcgag gcggagaggt gcggcggcag 720 ccaatcagag
cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg cggcggcggc 780
cctataaaaa gcgaagcgcg cggcgggcgg gagtcgctgc gacgctgcct tcgccccgtg
840 ccccgctccg ccgccgcctc gcgccgcccg ccccggctct gactgaccgc
gttactccca 900 caggtgagcg ggcgggacgg cccttctcct ccgggctgta
attagcgctt ggtttaatga 960 cggcttgttt cttttctgtg gctgcgtgaa
agccttgagg ggctccggga gggccctttg 1020 tgcgggggga gcggctcggg
gctgtccgcg gggggacggc tgccttcggg ggggacgggg 1080 cagggcgggg
ttcggcttct ggcgtgtgac cggcggctct agcagcctct gctaaccatg 1140
ttcatgcctt cttctttttc ctacagctcc tgggcaacgt gctggttatt gtgctgtctc
1200 atcattttgg caaagaatta agcttgagct cgcgatccgc agccatgact
gagacattgc 1260 caccggtaac ggaatcggct gttgcgctac aggcggaggt
gacccagagg gagctgttcg 1320 agttcgttct caacgacccc ctcctcgcca
gttcgctgta tattaatatc gcactggcag 1380 ggctgtcgat actgcttttc
gtgttcatga cgcgcggact cgacgaccca cgggcgaaac 1440 tcatcgccgt
ttcgacgatt ttggtgccgg tggtctctat cgcgagctac accggccttg 1500
catcggggct caccatcagc gtcctcgaga tgccagccgg ccacttcgcc gaggggtcct
1560 cggtgatgct cggcggcgaa gaggtagacg gcgtcgtgac gatgtggggc
cgctatctga 1620 cgtgggccct ttcgacaccg atgatactgc tggcgcttgg
gctgcttgct ggctctaacg 1680 ccacgaagct ctttaccgcc atcaccttcg
acatcgcgat gtgtgtcacc ggcctcgcag 1740 ccgcgctgac gacctcttcg
cacctgatgc ggtggttctg gtacgccatc agttgtgcgt 1800 gtttcctcgt
cgtcctctac atcctgctcg tcgagtgggc acaggacgcc aaggctgccg 1860
gtactgcgga tatgttcaat acgctgaagc tgctgaccgt tgtcatgtgg ctcggctacc
1920 ccatcgtgtg ggcactcggc gttgagggca tcgccgttct tccggtcgga
gtcacgtcgt 1980 ggggatacag cttcctcgac atcgtcgcga agtacatctt
cgcgttcctg ctgctcaact 2040 acctcacgtc gaacgagagc gtcgtctccg
gctcgatact cgacgtgccg tccgcgtcgg 2100 gcactcccgc tgacgacgaa
ttcggaggcg gaggtggagc tagcaaagga gaagaactct 2160 tcactggagt
tggagttgtc ccaattcttg ttgaattaga tggtgatgtt aacggccaca 2220
agttctctgt cagtggagag ggtgaaggtg atgcaacata cggaaaactt accctgaagt
2280 tcatctgcac tactggcaaa ctgcctgttc catggccaac actagtcact
actctgtgct 2340 atggtgttca atgcttttca agatacccgg atcatatgaa
acggcatgac tttttcaaga 2400 gtgccatgcc cgaaggttat gtacaggaaa
ggaccatctt cttcaaagat gacggcaact 2460 acaagacacg tgctgaagtc
aagtttgaag gtgataccct tgttaataga atcgagttaa 2520 aaggtattga
cttcaaggaa gatggcaaca ttctgggaca caaattggaa tacaactata 2580
actcacacaa tgtatacatc atggcagaca aacaaaagaa tggaatcaaa gtgaacttca
2640 agacccgcca caacattgaa gatggaagcg ttcaactagc agaccattat
caacaaaata 2700 ctccaattgg cgatggccct gtccttttac cagacaacca
ttacctgtcc acacaatctg 2760 ccctttcgaa agatcccaac gaaaagagag
accacatggt ccttcttgag tttgtaacag 2820 ctgctgggat tacacatggc
atggatgaac tgtacaacgt ggttcttcgg accgcctgtc 2880 ccccagatta
cacactagct atgaggaggt cacctgatga tgttccctta atgacaccca 2940
acaccattac aatgtaactc gagtctagac gtggtaccga taatcaacct ctggattaca
3000 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
ctatgtggat 3060 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
tatggctttc attttctcct 3120 ccttgtataa atcctggttg ctgtctcttt
atgaggagtt gtggcccgtt gtcaggcaac 3180 gtggcgtggt gtgcactgtg
tttgctgacg caacccccac tggttggggc attgccacca 3240 cctgtcagct
cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca 3300
tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
3360 tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt
gccacctgga 3420 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
caatccagcg gaccttcctt 3480 cccgcggcct gctgccggct ctgcggcctc
ttccgcgtct tcgccttcgc cctcagacga 3540 gtcggatctc cctttgggcc
gcctccccgc ctgatgcggg gatcctctag agtcgagaga 3600 tctacgggtg
gcatccctgt gacccctccc cagtgcctct cctggccctg gaagttgcca 3660
ctccagtgcc caccagcctt gtcctaataa aattaagttg catcattttg tctgactagg
3720 tgtccttcta taatattatg gggtggaggg gggtggtatg gagcaagggg
caagttggga 3780 agacaacctg tagggcctgc ggggtctatt gggaaccaag
ctggagtgca gtggcacaat 3840 cttggctcac tgcaatctcc gcctcctggg
ttcaagcgat tctcctgcct cagcctcccg 3900 agttgttggg attccaggca
tgcatgacca ggctcagcta atttttgttt ttttggtaga 3960 gacggggttt
caccatattg gccaggctgg tctccaactc ctaatctcag gtgatctacc 4020
caccttggcc tcccaaattg ctgggattac aggcgtgaac cactgctccc ttccctgtcc
4080 ttctgatttt gtaggtaacc acgtgcggac cgagcggccg caggaacccc
tagtgatgga 4140 gttggccact ccctctctgc gcgctcgctc gctcactgag
gccgggcgac caaaggtcgc 4200 ccgacgcccg ggctttgccc gggcggcctc
agtgagcgag cgagcgcgca gctgcctgca 4260 gg 4262 <210> SEQ ID NO
45 <211> LENGTH: 3626 <212> TYPE: DNA <213>
ORGANISM: Artificial Sequence <220> FEATURE: <223>
OTHER INFORMATION: 5' -ITR-CAG-HaloR-{NLG-1
Motif}-WPRE-bGHpolyA-ITR- 3' <400> SEQUENCE: 45 cctgcaggca
gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc 60
gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
120 actccatcac taggggttcc tgcggccgca cgcgtgatat cctagttatt
aatagtaatc 180 aattacgggg tcattagttc atagcccata tatggagttc
cgcgttacat aacttacggt 240 aaatggcccg cctggctgac cgcccaacga
cccccgccca ttgacgtcaa taatgacgta 300 tgttcccata gtaacgccaa
tagggacttt ccattgacgt caatgggtgg agtatttacg 360 gtaaactgcc
cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga 420
cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
480 tcctacttgg cagtacatct acgtattagt catcgctatt accatgcatg
gtcgaggtga 540 gccccacgtt ctgcttcact ctccccatct cccccccctc
cccaccccca attttgtatt 600 tatttatttt ttaattattt tgtgcagcga
tgggggcggg gggggggggg gggcgcgcgc 660 caggcggggc ggggcggggc
gaggggcggg gcggggcgag gcggagaggt gcggcggcag 720 ccaatcagag
cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg cggcggcggc 780
cctataaaaa gcgaagcgcg cggcgggcgg gagtcgctgc gacgctgcct tcgccccgtg
840 ccccgctccg ccgccgcctc gcgccgcccg ccccggctct gactgaccgc
gttactccca 900 caggtgagcg ggcgggacgg cccttctcct ccgggctgta
attagcgctt ggtttaatga 960 cggcttgttt cttttctgtg gctgcgtgaa
agccttgagg ggctccggga gggccctttg 1020 tgcgggggga gcggctcggg
gctgtccgcg gggggacggc tgccttcggg ggggacgggg 1080 cagggcgggg
ttcggcttct ggcgtgtgac cggcggctct agcagcctct gctaaccatg 1140
ttcatgcctt cttctttttc ctacagctcc tgggcaacgt gctggttatt gtgctgtctc
1200 atcattttgg caaagaatta agcttgagct cgcgatccgc agccatgact
gagacattgc 1260 caccggtaac ggaatcggct gttgcgctac aggcggaggt
gacccagagg gagctgttcg 1320 agttcgttct caacgacccc ctcctcgcca
gttcgctgta tattaatatc gcactggcag 1380 ggctgtcgat actgcttttc
gtgttcatga cgcgcggact cgacgaccca cgggcgaaac 1440 tcatcgccgt
ttcgacgatt ttggtgccgg tggtctctat cgcgagctac accggccttg 1500
catcggggct caccatcagc gtcctcgaga tgccagccgg ccacttcgcc gaggggtcct
1560 cggtgatgct cggcggcgaa gaggtagacg gcgtcgtgac gatgtggggc
cgctatctga 1620 cgtgggccct ttcgacaccg atgatactgc tggcgcttgg
gctgcttgct ggctctaacg 1680 ccacgaagct ctttaccgcc atcaccttcg
acatcgcgat gtgtgtcacc ggcctcgcag 1740 ccgcgctgac gacctcttcg
cacctgatgc ggtggttctg gtacgccatc agttgtgcgt 1800 gtttcctcgt
cgtcctctac atcctgctcg tcgagtgggc acaggacgcc aaggctgccg 1860
gtactgcgga tatgttcaat acgctgaagc tgctgaccgt tgtcatgtgg ctcggctacc
1920 ccatcgtgtg ggcactcggc gttgagggca tcgccgttct tccggtcgga
gtcacgtcgt 1980 ggggatacag cttcctcgac atcgtcgcga agtacatctt
cgcgttcctg ctgctcaact 2040 acctcacgtc gaacgagagc gtcgtctccg
gctcgatact cgacgtgccg tccgcgtcgg 2100 gcactcccgc tgacgacgaa
ttcggaggcg gaggtggagc tagcgtggtt cttcggaccg 2160 cctgtccccc
aaaaaagagg ctcctgagct tcagggacgt ggacttcgag gaggacagcg 2220
attacacact agctatgagg aggtcacctg atgatgttcc cttaatgaca cccaacacca
2280 ttacaatgta actcgagtct agacgtggta ccgataatca acctctggat
tacaaaattt 2340 gtgaaagatt gactggtatt cttaactatg ttgctccttt
tacgctatgt ggatacgctg 2400 ctttaatgcc tttgtatcat gctattgctt
cccgtatggc tttcattttc tcctccttgt 2460 ataaatcctg gttgctgtct
ctttatgagg agttgtggcc cgttgtcagg caacgtggcg 2520 tggtgtgcac
tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc 2580
agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
2640 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
tccgtggtgt 2700 tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg
tgttgccacc tggattctgc 2760 gcgggacgtc cttctgctac gtcccttcgg
ccctcaatcc agcggacctt ccttcccgcg 2820 gcctgctgcc ggctctgcgg
cctcttccgc gtcttcgcct tcgccctcag acgagtcgga 2880 tctccctttg
ggccgcctcc ccgcctgatg cggggatcct ctagagtcga gagatctacg 2940
ggtggcatcc ctgtgacccc tccccagtgc ctctcctggc cctggaagtt gccactccag
3000 tgcccaccag ccttgtccta ataaaattaa gttgcatcat tttgtctgac
taggtgtcct 3060 tctataatat tatggggtgg aggggggtgg tatggagcaa
ggggcaagtt gggaagacaa 3120 cctgtagggc ctgcggggtc tattgggaac
caagctggag tgcagtggca caatcttggc 3180 tcactgcaat ctccgcctcc
tgggttcaag cgattctcct gcctcagcct cccgagttgt 3240 tgggattcca
ggcatgcatg accaggctca gctaattttt gtttttttgg tagagacggg 3300
gtttcaccat attggccagg ctggtctcca actcctaatc tcaggtgatc tacccacctt
3360 ggcctcccaa attgctggga ttacaggcgt gaaccactgc tcccttccct
gtccttctga 3420 ttttgtaggt aaccacgtgc ggaccgagcg gccgcagggt
aaccacgtgc ggaccgagcg 3480 gccgcaggaa cccctagtga tggagttggc
cactccctct ctgcgcgctc gctcgctcac 3540 tgaggccggg cgaccaaagg
tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag 3600 cgagcgagcg
cgcagctgcc tgcagg 3626 <210> SEQ ID NO 46 <211> LENGTH:
4244 <212> TYPE: DNA <213> ORGANISM: Artificial
Sequence <220> FEATURE: <223> OTHER INFORMATION: 5'
-ITR-CAG-HaloR-GFP-{MLPH Motif}-WPRE-bGHpolyA-ITR- 3' <400>
SEQUENCE: 46 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag
cccgggcgtc 60 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc
gcgcagagag ggagtggcca 120 actccatcac taggggttcc tgcggccgca
cgcgtgatat cctagttatt aatagtaatc 180 aattacgggg tcattagttc
atagcccata tatggagttc cgcgttacat aacttacggt 240 aaatggcccg
cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta 300
tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
360 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
cccctattga 420 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
tacatgacct tatgggactt 480 tcctacttgg cagtacatct acgtattagt
catcgctatt accatgcatg gtcgaggtga 540 gccccacgtt ctgcttcact
ctccccatct cccccccctc cccaccccca attttgtatt 600 tatttatttt
ttaattattt tgtgcagcga tgggggcggg gggggggggg gggcgcgcgc 660
caggcggggc ggggcggggc gaggggcggg gcggggcgag gcggagaggt gcggcggcag
720 ccaatcagag cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg
cggcggcggc 780 cctataaaaa gcgaagcgcg cggcgggcgg gagtcgctgc
gacgctgcct tcgccccgtg 840 ccccgctccg ccgccgcctc gcgccgcccg
ccccggctct gactgaccgc gttactccca 900 caggtgagcg ggcgggacgg
cccttctcct ccgggctgta attagcgctt ggtttaatga 960 cggcttgttt
cttttctgtg gctgcgtgaa agccttgagg ggctccggga gggccctttg 1020
tgcgggggga gcggctcggg gctgtccgcg gggggacggc tgccttcggg ggggacgggg
1080 cagggcgggg ttcggcttct ggcgtgtgac cggcggctct agcagcctct
gctaaccatg 1140 ttcatgcctt cttctttttc ctacagctcc tgggcaacgt
gctggttatt gtgctgtctc 1200 atcattttgg caaagaatta agcttgagct
cgcgatccgc agccatgact gagacattgc 1260 caccggtaac ggaatcggct
gttgcgctac aggcggaggt gacccagagg gagctgttcg 1320 agttcgttct
caacgacccc ctcctcgcca gttcgctgta tattaatatc gcactggcag 1380
ggctgtcgat actgcttttc gtgttcatga cgcgcggact cgacgaccca cgggcgaaac
1440 tcatcgccgt ttcgacgatt ttggtgccgg tggtctctat cgcgagctac
accggccttg 1500 catcggggct caccatcagc gtcctcgaga tgccagccgg
ccacttcgcc gaggggtcct 1560 cggtgatgct cggcggcgaa gaggtagacg
gcgtcgtgac gatgtggggc cgctatctga 1620 cgtgggccct ttcgacaccg
atgatactgc tggcgcttgg gctgcttgct ggctctaacg 1680 ccacgaagct
ctttaccgcc atcaccttcg acatcgcgat gtgtgtcacc ggcctcgcag 1740
ccgcgctgac gacctcttcg cacctgatgc ggtggttctg gtacgccatc agttgtgcgt
1800 gtttcctcgt cgtcctctac atcctgctcg tcgagtgggc acaggacgcc
aaggctgccg 1860 gtactgcgga tatgttcaat acgctgaagc tgctgaccgt
tgtcatgtgg ctcggctacc 1920 ccatcgtgtg ggcactcggc gttgagggca
tcgccgttct tccggtcgga gtcacgtcgt 1980 ggggatacag cttcctcgac
atcgtcgcga agtacatctt cgcgttcctg ctgctcaact 2040 acctcacgtc
gaacgagagc gtcgtctccg gctcgatact cgacgtgccg tccgcgtcgg 2100
gcactcccgc tgacgacgaa ttcggaggcg gaggtggagc tagcaaagga gaagaactct
2160 tcactggagt tggagttgtc ccaattcttg ttgaattaga tggtgatgtt
aacggccaca 2220 agttctctgt cagtggagag ggtgaaggtg atgcaacata
cggaaaactt accctgaagt 2280 tcatctgcac tactggcaaa ctgcctgttc
catggccaac actagtcact actctgtgct 2340 atggtgttca atgcttttca
agatacccgg atcatatgaa acggcatgac tttttcaaga 2400 gtgccatgcc
cgaaggttat gtacaggaaa ggaccatctt cttcaaagat gacggcaact 2460
acaagacacg tgctgaagtc aagtttgaag gtgataccct tgttaataga atcgagttaa
2520 aaggtattga cttcaaggaa gatggcaaca ttctgggaca caaattggaa
tacaactata 2580 actcacacaa tgtatacatc atggcagaca aacaaaagaa
tggaatcaaa gtgaacttca 2640 agacccgcca caacattgaa gatggaagcg
ttcaactagc agaccattat caacaaaata 2700 ctccaattgg cgatggccct
gtccttttac cagacaacca ttacctgtcc acacaatctg 2760 ccctttcgaa
agatcccaac gaaaagagag accacatggt ccttcttgag tttgtaacag 2820
ctgctgggat tacacatggc atggatgaac tgtacaacag ggaccagcct ctgaacagca
2880 aaaagaaaaa gaggctcctg agcttcaggg acgtggactt cgaggaggac
agcgactaac 2940 tcgagtctag acgtggtacc gataatcaac ctctggatta
caaaatttgt gaaagattga 3000 ctggtattct taactatgtt gctcctttta
cgctatgtgg atacgctgct ttaatgcctt 3060 tgtatcatgc tattgcttcc
cgtatggctt tcattttctc ctccttgtat aaatcctggt 3120 tgctgtctct
ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg 3180
tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
3240 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
tgccttgccc 3300 gctgctggac aggggctcgg ctgttgggca ctgacaattc
cgtggtgttg tcggggaagc 3360 tgacgtcctt tccatggctg ctcgcctgtg
ttgccacctg gattctgcgc gggacgtcct 3420 tctgctacgt cccttcggcc
ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg 3480 ctctgcggcc
tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg 3540
ccgcctcccc gcctgatgcg gggatcctct agagtcgaga gatctacggg tggcatccct
3600 gtgacccctc cccagtgcct ctcctggccc tggaagttgc cactccagtg
cccaccagcc 3660 ttgtcctaat aaaattaagt tgcatcattt tgtctgacta
ggtgtccttc tataatatta 3720 tggggtggag gggggtggta tggagcaagg
ggcaagttgg gaagacaacc tgtagggcct 3780 gcggggtcta ttgggaacca
agctggagtg cagtggcaca atcttggctc actgcaatct 3840 ccgcctcctg
ggttcaagcg attctcctgc ctcagcctcc cgagttgttg ggattccagg 3900
catgcatgac caggctcagc taatttttgt ttttttggta gagacggggt ttcaccatat
3960 tggccaggct ggtctccaac tcctaatctc aggtgatcta cccaccttgg
cctcccaaat 4020 tgctgggatt acaggcgtga accactgctc ccttccctgt
ccttctgatt ttgtaggtaa 4080 ccacgtgcgg accgagcggc cgcaggaacc
cctagtgatg gagttggcca ctccctctct 4140 gcgcgctcgc tcgctcactg
aggccgggcg accaaaggtc gcccgacgcc cgggctttgc 4200 ccgggcggcc
tcagtgagcg agcgagcgcg cagctgcctg cagg 4244
<210> SEQ ID NO 47 <211> LENGTH: 3530 <212> TYPE:
DNA <213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: 5' -ITR-CAG-HaloR-{MLPH
Motif}-WPRE-bGHpoly A-ITR- 3' <400> SEQUENCE: 47 cctgcaggca
gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc 60
gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
120 actccatcac taggggttcc tgcggccgca cgcgtgatat cctagttatt
aatagtaatc 180 aattacgggg tcattagttc atagcccata tatggagttc
cgcgttacat aacttacggt 240 aaatggcccg cctggctgac cgcccaacga
cccccgccca ttgacgtcaa taatgacgta 300 tgttcccata gtaacgccaa
tagggacttt ccattgacgt caatgggtgg agtatttacg 360 gtaaactgcc
cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga 420
cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
480 tcctacttgg cagtacatct acgtattagt catcgctatt accatgcatg
gtcgaggtga 540 gccccacgtt ctgcttcact ctccccatct cccccccctc
cccaccccca attttgtatt 600 tatttatttt ttaattattt tgtgcagcga
tgggggcggg gggggggggg gggcgcgcgc 660 caggcggggc ggggcggggc
gaggggcggg gcggggcgag gcggagaggt gcggcggcag 720 ccaatcagag
cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg cggcggcggc 780
cctataaaaa gcgaagcgcg cggcgggcgg gagtcgctgc gacgctgcct tcgccccgtg
840 ccccgctccg ccgccgcctc gcgccgcccg ccccggctct gactgaccgc
gttactccca 900 caggtgagcg ggcgggacgg cccttctcct ccgggctgta
attagcgctt ggtttaatga 960 cggcttgttt cttttctgtg gctgcgtgaa
agccttgagg ggctccggga gggccctttg 1020 tgcgggggga gcggctcggg
gctgtccgcg gggggacggc tgccttcggg ggggacgggg 1080 cagggcgggg
ttcggcttct ggcgtgtgac cggcggctct agcagcctct gctaaccatg 1140
ttcatgcctt cttctttttc ctacagctcc tgggcaacgt gctggttatt gtgctgtctc
1200 atcattttgg caaagaatta agcttgagct cgcgatccgc agccatgact
gagacattgc 1260 caccggtaac ggaatcggct gttgcgctac aggcggaggt
gacccagagg gagctgttcg 1320 agttcgttct caacgacccc ctcctcgcca
gttcgctgta tattaatatc gcactggcag 1380 ggctgtcgat actgcttttc
gtgttcatga cgcgcggact cgacgaccca cgggcgaaac 1440 tcatcgccgt
ttcgacgatt ttggtgccgg tggtctctat cgcgagctac accggccttg 1500
catcggggct caccatcagc gtcctcgaga tgccagccgg ccacttcgcc gaggggtcct
1560 cggtgatgct cggcggcgaa gaggtagacg gcgtcgtgac gatgtggggc
cgctatctga 1620 cgtgggccct ttcgacaccg atgatactgc tggcgcttgg
gctgcttgct ggctctaacg 1680 ccacgaagct ctttaccgcc atcaccttcg
acatcgcgat gtgtgtcacc ggcctcgcag 1740 ccgcgctgac gacctcttcg
cacctgatgc ggtggttctg gtacgccatc agttgtgcgt 1800 gtttcctcgt
cgtcctctac atcctgctcg tcgagtgggc acaggacgcc aaggctgccg 1860
gtactgcgga tatgttcaat acgctgaagc tgctgaccgt tgtcatgtgg ctcggctacc
1920 ccatcgtgtg ggcactcggc gttgagggca tcgccgttct tccggtcgga
gtcacgtcgt 1980 ggggatacag cttcctcgac atcgtcgcga agtacatctt
cgcgttcctg ctgctcaact 2040 acctcacgtc gaacgagagc gtcgtctccg
gctcgatact cgacgtgccg tccgcgtcgg 2100 gcactcccgc tgacgacgaa
ttcggaggcg gaggtggagc tagcagggac cagcctctga 2160 acagcaaaaa
gaaaaagagg ctcctgagct tcagggacgt ggacttcgag gaggacagcg 2220
actaactcga gtctagacgt ggtaccgata atcaacctct ggattacaaa atttgtgaaa
2280 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
gctgctttaa 2340 tgcctttgta tcatgctatt gcttcccgta tggctttcat
tttctcctcc ttgtataaat 2400 cctggttgct gtctctttat gaggagttgt
ggcccgttgt caggcaacgt ggcgtggtgt 2460 gcactgtgtt tgctgacgca
acccccactg gttggggcat tgccaccacc tgtcagctcc 2520 tttccgggac
tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc 2580
ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
2640 ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt
ctgcgcggga 2700 cgtccttctg ctacgtccct tcggccctca atccagcgga
ccttccttcc cgcggcctgc 2760 tgccggctct gcggcctctt ccgcgtcttc
gccttcgccc tcagacgagt cggatctccc 2820 tttgggccgc ctccccgcct
gatgcgggga tcctctagag tcgagagatc tacgggtggc 2880 atccctgtga
cccctcccca gtgcctctcc tggccctgga agttgccact ccagtgccca 2940
ccagccttgt cctaataaaa ttaagttgca tcattttgtc tgactaggtg tccttctata
3000 atattatggg gtggaggggg gtggtatgga gcaaggggca agttgggaag
acaacctgta 3060 gggcctgcgg ggtctattgg gaaccaagct ggagtgcagt
ggcacaatct tggctcactg 3120 caatctccgc ctcctgggtt caagcgattc
tcctgcctca gcctcccgag ttgttgggat 3180 tccaggcatg catgaccagg
ctcagctaat ttttgttttt ttggtagaga cggggtttca 3240 ccatattggc
caggctggtc tccaactcct aatctcaggt gatctaccca ccttggcctc 3300
ccaaattgct gggattacag gcgtgaacca ctgctccctt ccctgtcctt ctgattttgt
3360 aggtaaccac gtgcggaccg agcggccgca ggaaccccta gtgatggagt
tggccactcc 3420 ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca
aaggtcgccc gacgcccggg 3480 ctttgcccgg gcggcctcag tgagcgagcg
agcgcgcagc tgcctgcagg 3530
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