U.S. patent application number 16/049169 was filed with the patent office on 2018-12-06 for synthetic genes. The applicant listed for this patent is DOW AGROSCIENCES LLC. Invention is credited to Ignacio Mario Larrinua, Donald J. Merlo, Avutu S. Reddy, Arvind Kumar ThirumalaiswamySekhar, Aaron T. Woosley.
Application Number | 20180346926 16/049169 |
Document ID | / |
Family ID | 47007465 |
Filed Date | 2018-12-06 |
United States Patent Application | 20180346926 |
Kind Code | A1 |
Larrinua; Ignacio Mario ; et al. | December 6, 2018 |
The invention provides synthetic nucleic acid sequences encoding proteins of interest that are particularly adapted to express well in plants. The claimed synthetic sequences utilize plant-optimized codons roughly in the same frequency at which they are utilized, on average, in genes naturally occurring in the plant species. The invention further includes synthetic DNA sequence for herbicide tolerance, water and/or heat stress tolerance, healthy oil modifications and for transformation marker genes and selectable marker genes are used. DNA construct and transgenic plants containing the synthetic sequences are taught as are methods and compositions for using the plants in agriculture.
Inventors: | Larrinua; Ignacio Mario; (Indianapolis, IN) ; Merlo; Donald J.; (Carmel, IN) ; Reddy; Avutu S.; (Carmel, IN) ; ThirumalaiswamySekhar; Arvind Kumar; (Zionsville, IN) ; Woosley; Aaron T.; (Fishers, IN) | ||||||||||
Applicant: |
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Family ID: | 47007465 | ||||||||||
Appl. No.: | 16/049169 | ||||||||||
Filed: | July 30, 2018 |
Application Number | Filing Date | Patent Number | ||
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15194925 | Jun 28, 2016 | |||
16049169 | ||||
13447836 | Apr 16, 2012 | 9427003 | ||
15194925 | ||||
61475921 | Apr 15, 2011 | |||
Current U.S. Class: | 1/1 |
Current CPC Class: | C07K 14/415 20130101; C12Y 111/01015 20130101; A23L 19/10 20160801; A23L 11/00 20160801; A23D 9/00 20130101; C07K 14/195 20130101; Y02A 40/162 20180101; C11B 1/00 20130101; C12Y 113/00 20130101; Y02A 40/146 20180101; C07K 14/325 20130101; C12N 9/0083 20130101; C12N 15/8286 20130101; A23L 7/198 20160801; C07K 14/37 20130101; A23L 11/05 20160801; A23L 7/10 20160801; C07K 14/38 20130101; C12N 9/0065 20130101; C07K 14/43595 20130101; C12N 9/0069 20130101 |
International Class: | C12N 15/82 20060101 C12N015/82; A23D 9/00 20060101 A23D009/00; C07K 14/415 20060101 C07K014/415; C12N 9/02 20060101 C12N009/02; C07K 14/195 20060101 C07K014/195; C07K 14/37 20060101 C07K014/37; C07K 14/38 20060101 C07K014/38; A23L 19/10 20060101 A23L019/10; A23L 11/00 20060101 A23L011/00; A23L 7/10 20060101 A23L007/10; C12N 9/08 20060101 C12N009/08; C11B 1/00 20060101 C11B001/00; C07K 14/435 20060101 C07K014/435; C07K 14/325 20060101 C07K014/325 |
Sequence CWU 1
1
7211818DNABacillus thuringiensisCDS(1)..(1818)Native DNA sequence
encoding Bacillus thuringiensis Cry1Fa core toxin 1atg gag aat aat
att caa aat caa tgc gta cct tac aat tgt tta aat 48Met Glu Asn Asn
Ile Gln Asn Gln Cys Val Pro Tyr Asn Cys Leu Asn 1 5 10 15 aat cct
gaa gta gaa ata tta aat gaa gaa aga agt act ggc aga tta 96Asn Pro
Glu Val Glu Ile Leu Asn Glu Glu Arg Ser Thr Gly Arg Leu 20 25 30
ccg tta gat ata tcc tta tcg ctt aca cgt ttc ctt ttg agt gaa ttt
144Pro Leu Asp Ile Ser Leu Ser Leu Thr Arg Phe Leu Leu Ser Glu Phe
35 40 45 gtt cca ggt gtg gga gtt gcg ttt gga tta ttt gat tta ata
tgg ggt 192Val Pro Gly Val Gly Val Ala Phe Gly Leu Phe Asp Leu Ile
Trp Gly 50 55 60 ttt ata act cct tct gat tgg agc tta ttt ctt tta
cag att gaa caa 240Phe Ile Thr Pro Ser Asp Trp Ser Leu Phe Leu Leu
Gln Ile Glu Gln 65 70 75 80 ttg att gag caa aga ata gaa aca ttg gaa
agg aac cgg gca att act 288Leu Ile Glu Gln Arg Ile Glu Thr Leu Glu
Arg Asn Arg Ala Ile Thr 85 90 95 aca tta cga ggg tta gca gat agc
tat gaa att tat att gaa gca cta 336Thr Leu Arg Gly Leu Ala Asp Ser
Tyr Glu Ile Tyr Ile Glu Ala Leu 100 105 110 aga gag tgg gaa gca aat
cct aat aat gca caa tta agg gaa gat gtg 384Arg Glu Trp Glu Ala Asn
Pro Asn Asn Ala Gln Leu Arg Glu Asp Val 115 120 125 cgt att cga ttt
gct aat aca gac gac gct tta ata aca gca ata aat 432Arg Ile Arg Phe
Ala Asn Thr Asp Asp Ala Leu Ile Thr Ala Ile Asn 130 135 140 aat ttt
aca ctt aca agt ttt gaa atc cct ctt tta tcg gtc tat gtt 480Asn Phe
Thr Leu Thr Ser Phe Glu Ile Pro Leu Leu Ser Val Tyr Val 145 150 155
160 caa gcg gcg aat tta cat tta tca cta tta aga gac gct gta tcg ttt
528Gln Ala Ala Asn Leu His Leu Ser Leu Leu Arg Asp Ala Val Ser Phe
165 170 175 ggg cag ggt tgg gga ctg gat ata gct act gtt aat aat cat
tat aat 576Gly Gln Gly Trp Gly Leu Asp Ile Ala Thr Val Asn Asn His
Tyr Asn 180 185 190 aga tta ata aat ctt att cat aga tat acg aaa cat
tgt ttg gac aca 624Arg Leu Ile Asn Leu Ile His Arg Tyr Thr Lys His
Cys Leu Asp Thr 195 200 205 tac aat caa gga tta gaa aac tta aga ggt
act aat act cga caa tgg 672Tyr Asn Gln Gly Leu Glu Asn Leu Arg Gly
Thr Asn Thr Arg Gln Trp 210 215 220 gca aga ttc aat cag ttt agg aga
gat tta aca ctt act gta tta gat 720Ala Arg Phe Asn Gln Phe Arg Arg
Asp Leu Thr Leu Thr Val Leu Asp 225 230 235 240 atc gtt gct ctt ttt
ccg aac tac gat gtt aga aca tat cca att caa 768Ile Val Ala Leu Phe
Pro Asn Tyr Asp Val Arg Thr Tyr Pro Ile Gln 245 250 255 acg tca tcc
caa tta aca agg gaa att tat aca agt tca gta att gag 816Thr Ser Ser
Gln Leu Thr Arg Glu Ile Tyr Thr Ser Ser Val Ile Glu 260 265 270 gat
tct cca gtt tct gct aat ata cct aat ggt ttt aat agg gcg gaa 864Asp
Ser Pro Val Ser Ala Asn Ile Pro Asn Gly Phe Asn Arg Ala Glu 275 280
285 ttt gga gtt aga ccg ccc cat ctt atg gac ttt atg aat tct ttg ttt
912Phe Gly Val Arg Pro Pro His Leu Met Asp Phe Met Asn Ser Leu Phe
290 295 300 gta act gca gag act gtt aga agt caa act gtg tgg gga gga
cac tta 960Val Thr Ala Glu Thr Val Arg Ser Gln Thr Val Trp Gly Gly
His Leu 305 310 315 320 gtt agt tca cga aat acg gct ggt aac cgt ata
aat ttc cct agt tac 1008Val Ser Ser Arg Asn Thr Ala Gly Asn Arg Ile
Asn Phe Pro Ser Tyr 325 330 335 ggg gtc ttc aat cct ggt ggc gcc att
tgg att gca gat gag gat cca 1056Gly Val Phe Asn Pro Gly Gly Ala Ile
Trp Ile Ala Asp Glu Asp Pro 340 345 350 cgt cct ttt tat cgg aca tta
tca gat cct gtt ttt gtc cga gga gga 1104Arg Pro Phe Tyr Arg Thr Leu
Ser Asp Pro Val Phe Val Arg Gly Gly 355 360 365 ttt ggg aat cct cat
tat gta ctg ggg ctt agg gga gta gca ttt caa 1152Phe Gly Asn Pro His
Tyr Val Leu Gly Leu Arg Gly Val Ala Phe Gln 370 375 380 caa act ggt
acg aac cac acc cga aca ttt aga aat agt ggg acc ata 1200Gln Thr Gly
Thr Asn His Thr Arg Thr Phe Arg Asn Ser Gly Thr Ile 385 390 395 400
gat tct cta gat gaa atc cca cct cag gat aat agt ggg gca cct tgg
1248Asp Ser Leu Asp Glu Ile Pro Pro Gln Asp Asn Ser Gly Ala Pro Trp
405 410 415 aat gat tat agt cat gta tta aat cat gtt aca ttt gta cga
tgg cca 1296Asn Asp Tyr Ser His Val Leu Asn His Val Thr Phe Val Arg
Trp Pro 420 425 430 ggt gag att tca gga agt gat tca tgg aga gct cca
atg ttt tct tgg 1344Gly Glu Ile Ser Gly Ser Asp Ser Trp Arg Ala Pro
Met Phe Ser Trp 435 440 445 acg cac cgt agt gca acc cct aca aat aca
att gat ccg gag agg att 1392Thr His Arg Ser Ala Thr Pro Thr Asn Thr
Ile Asp Pro Glu Arg Ile 450 455 460 act caa ata cca ttg gta aaa gca
cat aca ctt cag tca ggt act act 1440Thr Gln Ile Pro Leu Val Lys Ala
His Thr Leu Gln Ser Gly Thr Thr 465 470 475 480 gtt gta aga ggg ccc
ggg ttt acg gga gga gat att ctt cga cga aca 1488Val Val Arg Gly Pro
Gly Phe Thr Gly Gly Asp Ile Leu Arg Arg Thr 485 490 495 agt gga gga
cca ttt gct tat act att gtt aat ata aat ggg caa tta 1536Ser Gly Gly
Pro Phe Ala Tyr Thr Ile Val Asn Ile Asn Gly Gln Leu 500 505 510 ccc
caa agg tat cgt gca aga ata cgc tat gcc tct act aca aat cta 1584Pro
Gln Arg Tyr Arg Ala Arg Ile Arg Tyr Ala Ser Thr Thr Asn Leu 515 520
525 aga att tac gta acg gtt gca ggt gaa cgg att ttt gct ggt caa ttt
1632Arg Ile Tyr Val Thr Val Ala Gly Glu Arg Ile Phe Ala Gly Gln Phe
530 535 540 aac aaa aca atg gat acc ggt gac cca tta aca ttc caa tct
ttt agt 1680Asn Lys Thr Met Asp Thr Gly Asp Pro Leu Thr Phe Gln Ser
Phe Ser 545 550 555 560 tac gca act att aat aca gct ttt aca ttc cca
atg agc cag agt agt 1728Tyr Ala Thr Ile Asn Thr Ala Phe Thr Phe Pro
Met Ser Gln Ser Ser 565 570 575 ttc aca gta ggt gct gat act ttt agt
tca ggg aat gaa gtt tat ata 1776Phe Thr Val Gly Ala Asp Thr Phe Ser
Ser Gly Asn Glu Val Tyr Ile 580 585 590 gac aga ttt gaa ttg att cca
gtt act gca aca ttt gaa tag 1818Asp Arg Phe Glu Leu Ile Pro Val Thr
Ala Thr Phe Glu 595 600 605 2605PRTBacillus thuringiensis 2Met Glu
Asn Asn Ile Gln Asn Gln Cys Val Pro Tyr Asn Cys Leu Asn 1 5 10 15
Asn Pro Glu Val Glu Ile Leu Asn Glu Glu Arg Ser Thr Gly Arg Leu 20
25 30 Pro Leu Asp Ile Ser Leu Ser Leu Thr Arg Phe Leu Leu Ser Glu
Phe 35 40 45 Val Pro Gly Val Gly Val Ala Phe Gly Leu Phe Asp Leu
Ile Trp Gly 50 55 60 Phe Ile Thr Pro Ser Asp Trp Ser Leu Phe Leu
Leu Gln Ile Glu Gln 65 70 75 80 Leu Ile Glu Gln Arg Ile Glu Thr Leu
Glu Arg Asn Arg Ala Ile Thr 85 90 95 Thr Leu Arg Gly Leu Ala Asp
Ser Tyr Glu Ile Tyr Ile Glu Ala Leu 100 105 110 Arg Glu Trp Glu Ala
Asn Pro Asn Asn Ala Gln Leu Arg Glu Asp Val 115 120 125 Arg Ile Arg
Phe Ala Asn Thr Asp Asp Ala Leu Ile Thr Ala Ile Asn 130 135 140 Asn
Phe Thr Leu Thr Ser Phe Glu Ile Pro Leu Leu Ser Val Tyr Val 145 150
155 160 Gln Ala Ala Asn Leu His Leu Ser Leu Leu Arg Asp Ala Val Ser
Phe 165 170 175 Gly Gln Gly Trp Gly Leu Asp Ile Ala Thr Val Asn Asn
His Tyr Asn 180 185 190 Arg Leu Ile Asn Leu Ile His Arg Tyr Thr Lys
His Cys Leu Asp Thr 195 200 205 Tyr Asn Gln Gly Leu Glu Asn Leu Arg
Gly Thr Asn Thr Arg Gln Trp 210 215 220 Ala Arg Phe Asn Gln Phe Arg
Arg Asp Leu Thr Leu Thr Val Leu Asp 225 230 235 240 Ile Val Ala Leu
Phe Pro Asn Tyr Asp Val Arg Thr Tyr Pro Ile Gln 245 250 255 Thr Ser
Ser Gln Leu Thr Arg Glu Ile Tyr Thr Ser Ser Val Ile Glu 260 265 270
Asp Ser Pro Val Ser Ala Asn Ile Pro Asn Gly Phe Asn Arg Ala Glu 275
280 285 Phe Gly Val Arg Pro Pro His Leu Met Asp Phe Met Asn Ser Leu
Phe 290 295 300 Val Thr Ala Glu Thr Val Arg Ser Gln Thr Val Trp Gly
Gly His Leu 305 310 315 320 Val Ser Ser Arg Asn Thr Ala Gly Asn Arg
Ile Asn Phe Pro Ser Tyr 325 330 335 Gly Val Phe Asn Pro Gly Gly Ala
Ile Trp Ile Ala Asp Glu Asp Pro 340 345 350 Arg Pro Phe Tyr Arg Thr
Leu Ser Asp Pro Val Phe Val Arg Gly Gly 355 360 365 Phe Gly Asn Pro
His Tyr Val Leu Gly Leu Arg Gly Val Ala Phe Gln 370 375 380 Gln Thr
Gly Thr Asn His Thr Arg Thr Phe Arg Asn Ser Gly Thr Ile 385 390 395
400 Asp Ser Leu Asp Glu Ile Pro Pro Gln Asp Asn Ser Gly Ala Pro Trp
405 410 415 Asn Asp Tyr Ser His Val Leu Asn His Val Thr Phe Val Arg
Trp Pro 420 425 430 Gly Glu Ile Ser Gly Ser Asp Ser Trp Arg Ala Pro
Met Phe Ser Trp 435 440 445 Thr His Arg Ser Ala Thr Pro Thr Asn Thr
Ile Asp Pro Glu Arg Ile 450 455 460 Thr Gln Ile Pro Leu Val Lys Ala
His Thr Leu Gln Ser Gly Thr Thr 465 470 475 480 Val Val Arg Gly Pro
Gly Phe Thr Gly Gly Asp Ile Leu Arg Arg Thr 485 490 495 Ser Gly Gly
Pro Phe Ala Tyr Thr Ile Val Asn Ile Asn Gly Gln Leu 500 505 510 Pro
Gln Arg Tyr Arg Ala Arg Ile Arg Tyr Ala Ser Thr Thr Asn Leu 515 520
525 Arg Ile Tyr Val Thr Val Ala Gly Glu Arg Ile Phe Ala Gly Gln Phe
530 535 540 Asn Lys Thr Met Asp Thr Gly Asp Pro Leu Thr Phe Gln Ser
Phe Ser 545 550 555 560 Tyr Ala Thr Ile Asn Thr Ala Phe Thr Phe Pro
Met Ser Gln Ser Ser 565 570 575 Phe Thr Val Gly Ala Asp Thr Phe Ser
Ser Gly Asn Glu Val Tyr Ile 580 585 590 Asp Arg Phe Glu Leu Ile Pro
Val Thr Ala Thr Phe Glu 595 600 605 31818DNAArtificial
SequenceSynthetic DNA sequence encoding Bacillus thuringiensis
Cry1Fa core toxin using codons optimized for maize and Table 1
sequences are maintainedCDS(1)..(1818) 3atg gag aat aat atc cag aat
caa tgc gtg cct tac aat tgt tta aat 48Met Glu Asn Asn Ile Gln Asn
Gln Cys Val Pro Tyr Asn Cys Leu Asn 1 5 10 15 aat ccc gag gtg gag
ata tta aac gag gag aga tcc act ggc aga ctg 96Asn Pro Glu Val Glu
Ile Leu Asn Glu Glu Arg Ser Thr Gly Arg Leu 20 25 30 cca ctc gat
ata tcc ttg tcc ctt acc cgt ttc ctt ttg agc gaa ttt 144Pro Leu Asp
Ile Ser Leu Ser Leu Thr Arg Phe Leu Leu Ser Glu Phe 35 40 45 gtt
cct ggt gtg gga gtg gct ttc gga tta ttt gat ctg ata tgg ggt 192Val
Pro Gly Val Gly Val Ala Phe Gly Leu Phe Asp Leu Ile Trp Gly 50 55
60 ttt atc act cct tct gat tgg agc tta ttt ctt ctc cag att gag caa
240Phe Ile Thr Pro Ser Asp Trp Ser Leu Phe Leu Leu Gln Ile Glu Gln
65 70 75 80 ttg att gag cag aga ata gaa acc ttg gaa agg aac cgt gca
atc acg 288Leu Ile Glu Gln Arg Ile Glu Thr Leu Glu Arg Asn Arg Ala
Ile Thr 85 90 95 acc ttg cgc ggt ctc gcc gat agc tat gaa att tat
att gaa gca ctg 336Thr Leu Arg Gly Leu Ala Asp Ser Tyr Glu Ile Tyr
Ile Glu Ala Leu 100 105 110 agg gag tgg gag gcc aac cct aat aat gct
caa tta agg gaa gat gtg 384Arg Glu Trp Glu Ala Asn Pro Asn Asn Ala
Gln Leu Arg Glu Asp Val 115 120 125 cgt att cgt ttt gct aat aca gac
gac gct tta ata aca gca ata aat 432Arg Ile Arg Phe Ala Asn Thr Asp
Asp Ala Leu Ile Thr Ala Ile Asn 130 135 140 aat ttc aca ctt aca tcc
ttt gaa atc ccg ctt tta tca gtg tac gtt 480Asn Phe Thr Leu Thr Ser
Phe Glu Ile Pro Leu Leu Ser Val Tyr Val 145 150 155 160 caa gcc gcc
aat ctc cat tta tca ctt ctg agg gac gct gtc tcc ttt 528Gln Ala Ala
Asn Leu His Leu Ser Leu Leu Arg Asp Ala Val Ser Phe 165 170 175 ggg
caa ggt tgg gga ctg gat atc gct act gtt aat aat cac tac aat 576Gly
Gln Gly Trp Gly Leu Asp Ile Ala Thr Val Asn Asn His Tyr Asn 180 185
190 aga tta ata aac ctg att cat aga tat acg aag cat tgt ttg gac aca
624Arg Leu Ile Asn Leu Ile His Arg Tyr Thr Lys His Cys Leu Asp Thr
195 200 205 tac aat caa gga ctg gag aac ctt agg gga act aac act agg
cag tgg 672Tyr Asn Gln Gly Leu Glu Asn Leu Arg Gly Thr Asn Thr Arg
Gln Trp 210 215 220 gca agg ttc aac cag ttc aga cgt gat ctc aca ctt
act gtg ctg gat 720Ala Arg Phe Asn Gln Phe Arg Arg Asp Leu Thr Leu
Thr Val Leu Asp 225 230 235 240 atc gtt gct ctc ttt ccg aac tac gat
gtt cgc acc tac cca atc cag 768Ile Val Ala Leu Phe Pro Asn Tyr Asp
Val Arg Thr Tyr Pro Ile Gln 245 250 255 acg tca tcc caa tta aca agg
gaa att tat acc tcc tca gtg att gag 816Thr Ser Ser Gln Leu Thr Arg
Glu Ile Tyr Thr Ser Ser Val Ile Glu 260 265 270 gac tct ccc gtt tct
gct aac ata cct aac ggc ttc aac cgc gcc gag 864Asp Ser Pro Val Ser
Ala Asn Ile Pro Asn Gly Phe Asn Arg Ala Glu 275 280 285 ttc gga gtt
aga ccg ccc cac ctt atg gac ttt atg aat agc ttg ttt 912Phe Gly Val
Arg Pro Pro His Leu Met Asp Phe Met Asn Ser Leu Phe 290 295 300 gtg
act gct gag act gtt aga agc caa act gtg tgg ggc ggc cac ttg 960Val
Thr Ala Glu Thr Val Arg Ser Gln Thr Val Trp Gly Gly His Leu 305 310
315 320 gtc agc tca cgc aac acg gct ggc aac cgt atc aac ttc ccg tct
tac 1008Val Ser Ser Arg Asn Thr Ala Gly Asn Arg Ile Asn Phe Pro Ser
Tyr 325 330 335 ggg gtc ttt aac cct ggt ggc gcc att tgg att gca gac
gag gac cca 1056Gly Val Phe Asn Pro Gly Gly Ala Ile Trp Ile Ala Asp
Glu Asp Pro 340 345 350 cgt cct ttt tat cgc acc ctg tca gat cct gtt
ttt gtc aga ggc gga 1104Arg Pro Phe Tyr Arg Thr Leu Ser Asp Pro Val
Phe Val Arg Gly Gly 355 360 365 ttt ggg aat cct cat tat gtc ctg ggc
ctt agg gga gtg gct ttc caa 1152Phe Gly Asn Pro His Tyr Val Leu Gly
Leu Arg Gly Val Ala Phe Gln 370 375 380 cag act ggc acc aac cac acc
cgt acg ttt cgc aat agc ggg acc ata 1200Gln Thr Gly Thr Asn His Thr
Arg Thr Phe Arg Asn Ser Gly Thr Ile 385
390 395 400 gat tct ctt gat gaa atc cca cct caa gat aac agc ggc gca
cct tgg 1248Asp Ser Leu Asp Glu Ile Pro Pro Gln Asp Asn Ser Gly Ala
Pro Trp 405 410 415 aac gat tat tcc cac gta tta aat cac gtt acg ttc
gtc cgc tgg ccg 1296Asn Asp Tyr Ser His Val Leu Asn His Val Thr Phe
Val Arg Trp Pro 420 425 430 ggt gag atc agc ggc agc gat tca tgg aga
gca cca atg ttt tct tgg 1344Gly Glu Ile Ser Gly Ser Asp Ser Trp Arg
Ala Pro Met Phe Ser Trp 435 440 445 acg cac cgt tca gcc acc cct aca
aat aca att gac ccg gag agg att 1392Thr His Arg Ser Ala Thr Pro Thr
Asn Thr Ile Asp Pro Glu Arg Ile 450 455 460 act caa atc cca ttg gtc
aaa gca cat aca ctt cag tct ggg acc acc 1440Thr Gln Ile Pro Leu Val
Lys Ala His Thr Leu Gln Ser Gly Thr Thr 465 470 475 480 gtg gtc aga
ggg cct ggg ttc acg gga gga gac att ctt agg cgc aca 1488Val Val Arg
Gly Pro Gly Phe Thr Gly Gly Asp Ile Leu Arg Arg Thr 485 490 495 tcc
gga gga ccc ttc gct tat act atc gtt aat ata aat ggg cag ctc 1536Ser
Gly Gly Pro Phe Ala Tyr Thr Ile Val Asn Ile Asn Gly Gln Leu 500 505
510 ccc cag cgc tat cgt gcc aga atc cgt tac gcc tct act aca aat ctc
1584Pro Gln Arg Tyr Arg Ala Arg Ile Arg Tyr Ala Ser Thr Thr Asn Leu
515 520 525 aga atc tac gtg acg gtt gcc ggt gag cgc att ttt gct ggt
cag ttt 1632Arg Ile Tyr Val Thr Val Ala Gly Glu Arg Ile Phe Ala Gly
Gln Phe 530 535 540 aac aag acg atg gat act ggc gac cca ctg aca ttc
caa tct ttc tca 1680Asn Lys Thr Met Asp Thr Gly Asp Pro Leu Thr Phe
Gln Ser Phe Ser 545 550 555 560 tac gca act att aat aca gct ttc aca
ttc cca atg agc cag tca tct 1728Tyr Ala Thr Ile Asn Thr Ala Phe Thr
Phe Pro Met Ser Gln Ser Ser 565 570 575 ttc acc gtc ggt gct gat acc
ttc agc tct ggc aac gaa gtt tat ata 1776Phe Thr Val Gly Ala Asp Thr
Phe Ser Ser Gly Asn Glu Val Tyr Ile 580 585 590 gac aga ttt gag ttg
att cca gtt act gca acg ttt gag tga 1818Asp Arg Phe Glu Leu Ile Pro
Val Thr Ala Thr Phe Glu 595 600 605 4605PRTArtificial
SequenceSynthetic Construct 4Met Glu Asn Asn Ile Gln Asn Gln Cys
Val Pro Tyr Asn Cys Leu Asn 1 5 10 15 Asn Pro Glu Val Glu Ile Leu
Asn Glu Glu Arg Ser Thr Gly Arg Leu 20 25 30 Pro Leu Asp Ile Ser
Leu Ser Leu Thr Arg Phe Leu Leu Ser Glu Phe 35 40 45 Val Pro Gly
Val Gly Val Ala Phe Gly Leu Phe Asp Leu Ile Trp Gly 50 55 60 Phe
Ile Thr Pro Ser Asp Trp Ser Leu Phe Leu Leu Gln Ile Glu Gln 65 70
75 80 Leu Ile Glu Gln Arg Ile Glu Thr Leu Glu Arg Asn Arg Ala Ile
Thr 85 90 95 Thr Leu Arg Gly Leu Ala Asp Ser Tyr Glu Ile Tyr Ile
Glu Ala Leu 100 105 110 Arg Glu Trp Glu Ala Asn Pro Asn Asn Ala Gln
Leu Arg Glu Asp Val 115 120 125 Arg Ile Arg Phe Ala Asn Thr Asp Asp
Ala Leu Ile Thr Ala Ile Asn 130 135 140 Asn Phe Thr Leu Thr Ser Phe
Glu Ile Pro Leu Leu Ser Val Tyr Val 145 150 155 160 Gln Ala Ala Asn
Leu His Leu Ser Leu Leu Arg Asp Ala Val Ser Phe 165 170 175 Gly Gln
Gly Trp Gly Leu Asp Ile Ala Thr Val Asn Asn His Tyr Asn 180 185 190
Arg Leu Ile Asn Leu Ile His Arg Tyr Thr Lys His Cys Leu Asp Thr 195
200 205 Tyr Asn Gln Gly Leu Glu Asn Leu Arg Gly Thr Asn Thr Arg Gln
Trp 210 215 220 Ala Arg Phe Asn Gln Phe Arg Arg Asp Leu Thr Leu Thr
Val Leu Asp 225 230 235 240 Ile Val Ala Leu Phe Pro Asn Tyr Asp Val
Arg Thr Tyr Pro Ile Gln 245 250 255 Thr Ser Ser Gln Leu Thr Arg Glu
Ile Tyr Thr Ser Ser Val Ile Glu 260 265 270 Asp Ser Pro Val Ser Ala
Asn Ile Pro Asn Gly Phe Asn Arg Ala Glu 275 280 285 Phe Gly Val Arg
Pro Pro His Leu Met Asp Phe Met Asn Ser Leu Phe 290 295 300 Val Thr
Ala Glu Thr Val Arg Ser Gln Thr Val Trp Gly Gly His Leu 305 310 315
320 Val Ser Ser Arg Asn Thr Ala Gly Asn Arg Ile Asn Phe Pro Ser Tyr
325 330 335 Gly Val Phe Asn Pro Gly Gly Ala Ile Trp Ile Ala Asp Glu
Asp Pro 340 345 350 Arg Pro Phe Tyr Arg Thr Leu Ser Asp Pro Val Phe
Val Arg Gly Gly 355 360 365 Phe Gly Asn Pro His Tyr Val Leu Gly Leu
Arg Gly Val Ala Phe Gln 370 375 380 Gln Thr Gly Thr Asn His Thr Arg
Thr Phe Arg Asn Ser Gly Thr Ile 385 390 395 400 Asp Ser Leu Asp Glu
Ile Pro Pro Gln Asp Asn Ser Gly Ala Pro Trp 405 410 415 Asn Asp Tyr
Ser His Val Leu Asn His Val Thr Phe Val Arg Trp Pro 420 425 430 Gly
Glu Ile Ser Gly Ser Asp Ser Trp Arg Ala Pro Met Phe Ser Trp 435 440
445 Thr His Arg Ser Ala Thr Pro Thr Asn Thr Ile Asp Pro Glu Arg Ile
450 455 460 Thr Gln Ile Pro Leu Val Lys Ala His Thr Leu Gln Ser Gly
Thr Thr 465 470 475 480 Val Val Arg Gly Pro Gly Phe Thr Gly Gly Asp
Ile Leu Arg Arg Thr 485 490 495 Ser Gly Gly Pro Phe Ala Tyr Thr Ile
Val Asn Ile Asn Gly Gln Leu 500 505 510 Pro Gln Arg Tyr Arg Ala Arg
Ile Arg Tyr Ala Ser Thr Thr Asn Leu 515 520 525 Arg Ile Tyr Val Thr
Val Ala Gly Glu Arg Ile Phe Ala Gly Gln Phe 530 535 540 Asn Lys Thr
Met Asp Thr Gly Asp Pro Leu Thr Phe Gln Ser Phe Ser 545 550 555 560
Tyr Ala Thr Ile Asn Thr Ala Phe Thr Phe Pro Met Ser Gln Ser Ser 565
570 575 Phe Thr Val Gly Ala Asp Thr Phe Ser Ser Gly Asn Glu Val Tyr
Ile 580 585 590 Asp Arg Phe Glu Leu Ile Pro Val Thr Ala Thr Phe Glu
595 600 605 51818DNAArtificial SequenceSynthetic DNA sequence in
accordance with the invention encoding Bacillus thuringiensis
Cry1Fa core toxin using codons optimized for maize and with
sequences identified in Table 2 removed and Table 1 sequences are
maintainedCDS(1)..(1818) 5atg gag aat aat atc cag aat caa tgc gtg
cct tac aat tgt ctc aat 48Met Glu Asn Asn Ile Gln Asn Gln Cys Val
Pro Tyr Asn Cys Leu Asn 1 5 10 15 aat ccc gag gtg gag ata tta aac
gag gag aga tcc act ggc aga ctg 96Asn Pro Glu Val Glu Ile Leu Asn
Glu Glu Arg Ser Thr Gly Arg Leu 20 25 30 cca ctc gac ata tcc ttg
tcc ctt acc cgt ttc ctt ttg agc gaa ttt 144Pro Leu Asp Ile Ser Leu
Ser Leu Thr Arg Phe Leu Leu Ser Glu Phe 35 40 45 gtt cct ggt gtg
gga gtg gct ttc gga ctg ttc gat ctg ata tgg ggc 192Val Pro Gly Val
Gly Val Ala Phe Gly Leu Phe Asp Leu Ile Trp Gly 50 55 60 ttt atc
act cct tct gat tgg agc ctc ttc ctt ctc cag att gag caa 240Phe Ile
Thr Pro Ser Asp Trp Ser Leu Phe Leu Leu Gln Ile Glu Gln 65 70 75 80
ttg att gag cag aga ata gaa acc ttg gaa agg aac cgt gca atc acg
288Leu Ile Glu Gln Arg Ile Glu Thr Leu Glu Arg Asn Arg Ala Ile Thr
85 90 95 acc ttg cgc ggt ctc gcc gat agc tat gaa atc tac att gaa
gca ctg 336Thr Leu Arg Gly Leu Ala Asp Ser Tyr Glu Ile Tyr Ile Glu
Ala Leu 100 105 110 agg gag tgg gag gcc aac ccc aat aat gct caa tta
agg gaa gat gtg 384Arg Glu Trp Glu Ala Asn Pro Asn Asn Ala Gln Leu
Arg Glu Asp Val 115 120 125 cgt att cgt ttt gct aat aca gac gac gct
ctc atc aca gca atc aat 432Arg Ile Arg Phe Ala Asn Thr Asp Asp Ala
Leu Ile Thr Ala Ile Asn 130 135 140 aat ttc aca ctt aca tcc ttt gaa
atc ccg ctt ttg agc gtg tac gtt 480Asn Phe Thr Leu Thr Ser Phe Glu
Ile Pro Leu Leu Ser Val Tyr Val 145 150 155 160 caa gcc gcc aat ctc
cac ctc tca ctt ctg agg gac gct gtc tcc ttt 528Gln Ala Ala Asn Leu
His Leu Ser Leu Leu Arg Asp Ala Val Ser Phe 165 170 175 ggg caa ggt
tgg gga ctg gat atc gct act gtg aat aat cac tac aat 576Gly Gln Gly
Trp Gly Leu Asp Ile Ala Thr Val Asn Asn His Tyr Asn 180 185 190 aga
tta atc aac ctg att cat aga tat acg aag cac tgc ttg gac aca 624Arg
Leu Ile Asn Leu Ile His Arg Tyr Thr Lys His Cys Leu Asp Thr 195 200
205 tac aat caa gga ctg gag aac ctt agg gga act aac act agg cag tgg
672Tyr Asn Gln Gly Leu Glu Asn Leu Arg Gly Thr Asn Thr Arg Gln Trp
210 215 220 gca agg ttc aac cag ttc aga cgt gat ctc aca ctt act gtg
ctg gat 720Ala Arg Phe Asn Gln Phe Arg Arg Asp Leu Thr Leu Thr Val
Leu Asp 225 230 235 240 atc gtt gct ctc ttt ccg aac tac gat gtt cgc
acc tac cca atc cag 768Ile Val Ala Leu Phe Pro Asn Tyr Asp Val Arg
Thr Tyr Pro Ile Gln 245 250 255 acg tca tcc caa tta aca agg gaa atc
tac acc tcc tca gtg att gag 816Thr Ser Ser Gln Leu Thr Arg Glu Ile
Tyr Thr Ser Ser Val Ile Glu 260 265 270 gac tct ccc gtt tct gct aac
ata cct aac ggc ttc aac cgc gcc gag 864Asp Ser Pro Val Ser Ala Asn
Ile Pro Asn Gly Phe Asn Arg Ala Glu 275 280 285 ttc gga gtt aga ccg
ccc cac ctt atg gac ttt atg aat agc ttg ttc 912Phe Gly Val Arg Pro
Pro His Leu Met Asp Phe Met Asn Ser Leu Phe 290 295 300 gtg act gct
gag act gtt aga agc caa act gtg tgg ggc ggc cac ttg 960Val Thr Ala
Glu Thr Val Arg Ser Gln Thr Val Trp Gly Gly His Leu 305 310 315 320
gtc agc tca cgc aac acg gct ggc aac cgt atc aac ttc ccg tct tac
1008Val Ser Ser Arg Asn Thr Ala Gly Asn Arg Ile Asn Phe Pro Ser Tyr
325 330 335 ggg gtc ttt aac cct ggt ggc gcc att tgg att gca gac gag
gac cca 1056Gly Val Phe Asn Pro Gly Gly Ala Ile Trp Ile Ala Asp Glu
Asp Pro 340 345 350 cgt cct ttt tac cgc acc ctg tca gat ccg gtt ttc
gtc aga ggc gga 1104Arg Pro Phe Tyr Arg Thr Leu Ser Asp Pro Val Phe
Val Arg Gly Gly 355 360 365 ttt ggg aat cct cat tat gtc ctg ggc ctt
agg gga gtg gct ttc caa 1152Phe Gly Asn Pro His Tyr Val Leu Gly Leu
Arg Gly Val Ala Phe Gln 370 375 380 cag act ggc acc aac cac acc cgt
acg ttt cgc aat agc ggg acc ata 1200Gln Thr Gly Thr Asn His Thr Arg
Thr Phe Arg Asn Ser Gly Thr Ile 385 390 395 400 gat tct ctt gat gaa
atc cca cct caa gat aac agc ggc gca cct tgg 1248Asp Ser Leu Asp Glu
Ile Pro Pro Gln Asp Asn Ser Gly Ala Pro Trp 405 410 415 aac gat tat
tcc cac gta tta aat cac gtt acg ttc gtc cgc tgg ccg 1296Asn Asp Tyr
Ser His Val Leu Asn His Val Thr Phe Val Arg Trp Pro 420 425 430 ggt
gag atc agc ggc agc gat tca tgg aga gca cca atg ttc tct tgg 1344Gly
Glu Ile Ser Gly Ser Asp Ser Trp Arg Ala Pro Met Phe Ser Trp 435 440
445 acg cac cgt tca gcc acc cct aca aat aca att gac ccg gag agg att
1392Thr His Arg Ser Ala Thr Pro Thr Asn Thr Ile Asp Pro Glu Arg Ile
450 455 460 act caa atc cca ttg gtc aaa gca cat aca ctt cag tct ggg
acc acc 1440Thr Gln Ile Pro Leu Val Lys Ala His Thr Leu Gln Ser Gly
Thr Thr 465 470 475 480 gtg gtc aga ggg cct ggg ttc acg gga gga gac
att ctt agg cgc aca 1488Val Val Arg Gly Pro Gly Phe Thr Gly Gly Asp
Ile Leu Arg Arg Thr 485 490 495 tcc gga gga ccc ttc gct tat act atc
gtt aat ata aat ggg cag ctc 1536Ser Gly Gly Pro Phe Ala Tyr Thr Ile
Val Asn Ile Asn Gly Gln Leu 500 505 510 ccc cag cgc tat cgt gcc aga
atc cgt tac gcc tct act aca aat ctc 1584Pro Gln Arg Tyr Arg Ala Arg
Ile Arg Tyr Ala Ser Thr Thr Asn Leu 515 520 525 aga atc tac gtg acg
gtt gcc ggt gag cgc atc ttt gct ggt cag ttt 1632Arg Ile Tyr Val Thr
Val Ala Gly Glu Arg Ile Phe Ala Gly Gln Phe 530 535 540 aac aag acg
atg gat act ggc gac cca ctg aca ttc caa tct ttc tca 1680Asn Lys Thr
Met Asp Thr Gly Asp Pro Leu Thr Phe Gln Ser Phe Ser 545 550 555 560
tac gca act att aat aca gct ttc aca ttc cca atg agc cag tca tct
1728Tyr Ala Thr Ile Asn Thr Ala Phe Thr Phe Pro Met Ser Gln Ser Ser
565 570 575 ttc acc gtc ggt gct gat acc ttc agc tct ggc aac gaa gtc
tat atc 1776Phe Thr Val Gly Ala Asp Thr Phe Ser Ser Gly Asn Glu Val
Tyr Ile 580 585 590 gac aga ttt gag ttg att cca gtt act gca acg ttt
gag tga 1818Asp Arg Phe Glu Leu Ile Pro Val Thr Ala Thr Phe Glu 595
600 605 6605PRTArtificial SequenceSynthetic Construct 6Met Glu Asn
Asn Ile Gln Asn Gln Cys Val Pro Tyr Asn Cys Leu Asn 1 5 10 15 Asn
Pro Glu Val Glu Ile Leu Asn Glu Glu Arg Ser Thr Gly Arg Leu 20 25
30 Pro Leu Asp Ile Ser Leu Ser Leu Thr Arg Phe Leu Leu Ser Glu Phe
35 40 45 Val Pro Gly Val Gly Val Ala Phe Gly Leu Phe Asp Leu Ile
Trp Gly 50 55 60 Phe Ile Thr Pro Ser Asp Trp Ser Leu Phe Leu Leu
Gln Ile Glu Gln 65 70 75 80 Leu Ile Glu Gln Arg Ile Glu Thr Leu Glu
Arg Asn Arg Ala Ile Thr 85 90 95 Thr Leu Arg Gly Leu Ala Asp Ser
Tyr Glu Ile Tyr Ile Glu Ala Leu 100 105 110 Arg Glu Trp Glu Ala Asn
Pro Asn Asn Ala Gln Leu Arg Glu Asp Val 115 120 125 Arg Ile Arg Phe
Ala Asn Thr Asp Asp Ala Leu Ile Thr Ala Ile Asn 130 135 140 Asn Phe
Thr Leu Thr Ser Phe Glu Ile Pro Leu Leu Ser Val Tyr Val 145 150 155
160 Gln Ala Ala Asn Leu His Leu Ser Leu Leu Arg Asp Ala Val Ser Phe
165 170 175 Gly Gln Gly Trp Gly Leu Asp Ile Ala Thr Val Asn Asn His
Tyr Asn 180 185 190 Arg Leu Ile Asn Leu Ile His Arg Tyr Thr Lys His
Cys Leu Asp Thr 195 200 205 Tyr Asn Gln Gly Leu Glu Asn Leu Arg Gly
Thr Asn Thr Arg Gln Trp 210 215 220 Ala Arg Phe Asn Gln Phe Arg Arg
Asp Leu Thr Leu Thr Val Leu Asp 225 230 235 240 Ile Val Ala Leu Phe
Pro Asn Tyr Asp Val Arg Thr Tyr Pro Ile Gln 245 250 255 Thr Ser Ser
Gln Leu Thr Arg Glu Ile Tyr Thr Ser Ser Val Ile Glu 260 265 270 Asp
Ser Pro Val Ser Ala Asn Ile Pro Asn Gly Phe Asn Arg Ala Glu 275 280
285 Phe Gly Val Arg Pro Pro His Leu Met Asp Phe Met Asn Ser Leu Phe
290 295
300 Val Thr Ala Glu Thr Val Arg Ser Gln Thr Val Trp Gly Gly His Leu
305 310 315 320 Val Ser Ser Arg Asn Thr Ala Gly Asn Arg Ile Asn Phe
Pro Ser Tyr 325 330 335 Gly Val Phe Asn Pro Gly Gly Ala Ile Trp Ile
Ala Asp Glu Asp Pro 340 345 350 Arg Pro Phe Tyr Arg Thr Leu Ser Asp
Pro Val Phe Val Arg Gly Gly 355 360 365 Phe Gly Asn Pro His Tyr Val
Leu Gly Leu Arg Gly Val Ala Phe Gln 370 375 380 Gln Thr Gly Thr Asn
His Thr Arg Thr Phe Arg Asn Ser Gly Thr Ile 385 390 395 400 Asp Ser
Leu Asp Glu Ile Pro Pro Gln Asp Asn Ser Gly Ala Pro Trp 405 410 415
Asn Asp Tyr Ser His Val Leu Asn His Val Thr Phe Val Arg Trp Pro 420
425 430 Gly Glu Ile Ser Gly Ser Asp Ser Trp Arg Ala Pro Met Phe Ser
Trp 435 440 445 Thr His Arg Ser Ala Thr Pro Thr Asn Thr Ile Asp Pro
Glu Arg Ile 450 455 460 Thr Gln Ile Pro Leu Val Lys Ala His Thr Leu
Gln Ser Gly Thr Thr 465 470 475 480 Val Val Arg Gly Pro Gly Phe Thr
Gly Gly Asp Ile Leu Arg Arg Thr 485 490 495 Ser Gly Gly Pro Phe Ala
Tyr Thr Ile Val Asn Ile Asn Gly Gln Leu 500 505 510 Pro Gln Arg Tyr
Arg Ala Arg Ile Arg Tyr Ala Ser Thr Thr Asn Leu 515 520 525 Arg Ile
Tyr Val Thr Val Ala Gly Glu Arg Ile Phe Ala Gly Gln Phe 530 535 540
Asn Lys Thr Met Asp Thr Gly Asp Pro Leu Thr Phe Gln Ser Phe Ser 545
550 555 560 Tyr Ala Thr Ile Asn Thr Ala Phe Thr Phe Pro Met Ser Gln
Ser Ser 565 570 575 Phe Thr Val Gly Ala Asp Thr Phe Ser Ser Gly Asn
Glu Val Tyr Ile 580 585 590 Asp Arg Phe Glu Leu Ile Pro Val Thr Ala
Thr Phe Glu 595 600 605 7372DNABacillus
thuringiensisCDS(1)..(372)Native DNA sequence encoding Bacillus
thuringiensis Cry34Ab1 toxin 7atg tca gca cgt gaa gta cac att gat
gta aat aat aag aca ggt cat 48Met Ser Ala Arg Glu Val His Ile Asp
Val Asn Asn Lys Thr Gly His 1 5 10 15 aca tta caa tta gaa gat aaa
aca aaa ctt gat ggt ggt aga tgg cga 96Thr Leu Gln Leu Glu Asp Lys
Thr Lys Leu Asp Gly Gly Arg Trp Arg 20 25 30 aca tca cct aca aat
gtt gct aat gat caa att aaa aca ttt gta gca 144Thr Ser Pro Thr Asn
Val Ala Asn Asp Gln Ile Lys Thr Phe Val Ala 35 40 45 gaa tca aat
ggt ttt atg aca ggt aca gaa ggt act ata tat tat agt 192Glu Ser Asn
Gly Phe Met Thr Gly Thr Glu Gly Thr Ile Tyr Tyr Ser 50 55 60 ata
aat gga gaa gca gaa att agt tta tat ttt gac aat cct ttt gca 240Ile
Asn Gly Glu Ala Glu Ile Ser Leu Tyr Phe Asp Asn Pro Phe Ala 65 70
75 80 ggt tct aat aaa tat gat gga cat tcc aat aaa tct caa tat gaa
att 288Gly Ser Asn Lys Tyr Asp Gly His Ser Asn Lys Ser Gln Tyr Glu
Ile 85 90 95 att acc caa gga gga tca gga aat caa tct cat gtt acg
tat act att 336Ile Thr Gln Gly Gly Ser Gly Asn Gln Ser His Val Thr
Tyr Thr Ile 100 105 110 caa acc aca tcc tca cga tat ggg cat aaa tca
taa 372Gln Thr Thr Ser Ser Arg Tyr Gly His Lys Ser 115 120
8123PRTBacillus thuringiensis 8Met Ser Ala Arg Glu Val His Ile Asp
Val Asn Asn Lys Thr Gly His 1 5 10 15 Thr Leu Gln Leu Glu Asp Lys
Thr Lys Leu Asp Gly Gly Arg Trp Arg 20 25 30 Thr Ser Pro Thr Asn
Val Ala Asn Asp Gln Ile Lys Thr Phe Val Ala 35 40 45 Glu Ser Asn
Gly Phe Met Thr Gly Thr Glu Gly Thr Ile Tyr Tyr Ser 50 55 60 Ile
Asn Gly Glu Ala Glu Ile Ser Leu Tyr Phe Asp Asn Pro Phe Ala 65 70
75 80 Gly Ser Asn Lys Tyr Asp Gly His Ser Asn Lys Ser Gln Tyr Glu
Ile 85 90 95 Ile Thr Gln Gly Gly Ser Gly Asn Gln Ser His Val Thr
Tyr Thr Ile 100 105 110 Gln Thr Thr Ser Ser Arg Tyr Gly His Lys Ser
115 120 9372DNAArtificial SequenceSynthetic DNA sequence encoding
Bacillus thuringiensis Cry34Ab1 toxin using codons optimized for
maize and Table 1 sequences are maintainedCDS(1)..(372) 9atg tca
gca cgg gag gtc cac atc gat gta aat aat aag acg ggt cat 48Met Ser
Ala Arg Glu Val His Ile Asp Val Asn Asn Lys Thr Gly His 1 5 10 15
aca tta cag ttg gag gat aaa aca aag cta gac ggt ggc aga tgg aga
96Thr Leu Gln Leu Glu Asp Lys Thr Lys Leu Asp Gly Gly Arg Trp Arg
20 25 30 acc agt ccg acc aac gtt gct aac gat caa att aaa aca ttt
gta gcc 144Thr Ser Pro Thr Asn Val Ala Asn Asp Gln Ile Lys Thr Phe
Val Ala 35 40 45 gaa tca aac ggt ttt atg act ggc acg gag ggg act
ata tat tat tcc 192Glu Ser Asn Gly Phe Met Thr Gly Thr Glu Gly Thr
Ile Tyr Tyr Ser 50 55 60 atc aac gga gaa gcc gag att tcg tta tat
ttt gac aat cca ttc gcg 240Ile Asn Gly Glu Ala Glu Ile Ser Leu Tyr
Phe Asp Asn Pro Phe Ala 65 70 75 80 ggg tct aat aaa tac gac gga cac
tcc aat aaa tct caa tat gaa atc 288Gly Ser Asn Lys Tyr Asp Gly His
Ser Asn Lys Ser Gln Tyr Glu Ile 85 90 95 att aca caa ggc ggc agc
gga aat caa agc cac gtc acg tat act atc 336Ile Thr Gln Gly Gly Ser
Gly Asn Gln Ser His Val Thr Tyr Thr Ile 100 105 110 cag acc act tca
tcg cgc tac ggg cat aaa tca tag 372Gln Thr Thr Ser Ser Arg Tyr Gly
His Lys Ser 115 120 10123PRTArtificial SequenceSynthetic Construct
10Met Ser Ala Arg Glu Val His Ile Asp Val Asn Asn Lys Thr Gly His 1
5 10 15 Thr Leu Gln Leu Glu Asp Lys Thr Lys Leu Asp Gly Gly Arg Trp
Arg 20 25 30 Thr Ser Pro Thr Asn Val Ala Asn Asp Gln Ile Lys Thr
Phe Val Ala 35 40 45 Glu Ser Asn Gly Phe Met Thr Gly Thr Glu Gly
Thr Ile Tyr Tyr Ser 50 55 60 Ile Asn Gly Glu Ala Glu Ile Ser Leu
Tyr Phe Asp Asn Pro Phe Ala 65 70 75 80 Gly Ser Asn Lys Tyr Asp Gly
His Ser Asn Lys Ser Gln Tyr Glu Ile 85 90 95 Ile Thr Gln Gly Gly
Ser Gly Asn Gln Ser His Val Thr Tyr Thr Ile 100 105 110 Gln Thr Thr
Ser Ser Arg Tyr Gly His Lys Ser 115 120 11372DNAArtificial
SequenceSynthetic DNA sequence in accordance with the invention
encoding Bacillus thuringiensis Cry34Ab1 toxin using codons
optimized for maize and with sequences identified in Table 2
removed and Table 1 sequences are maintainedCDS(1)..(372) 11atg tca
gca cgg gag gtc cac atc gat gta aat aat aag acg ggt cat 48Met Ser
Ala Arg Glu Val His Ile Asp Val Asn Asn Lys Thr Gly His 1 5 10 15
aca tta cag ttg gag gat aaa aca aag cta gac ggt ggc aga tgg aga
96Thr Leu Gln Leu Glu Asp Lys Thr Lys Leu Asp Gly Gly Arg Trp Arg
20 25 30 acc agt ccg acc aac gtt gct aac gat caa att aaa aca ttt
gta gcc 144Thr Ser Pro Thr Asn Val Ala Asn Asp Gln Ile Lys Thr Phe
Val Ala 35 40 45 gaa tca aac ggt ttc atg act ggc acg gag ggg act
atc tac tac tcc 192Glu Ser Asn Gly Phe Met Thr Gly Thr Glu Gly Thr
Ile Tyr Tyr Ser 50 55 60 atc aac gga gaa gcc gag att tcg ctg tac
ttc gac aat cca ttc gcg 240Ile Asn Gly Glu Ala Glu Ile Ser Leu Tyr
Phe Asp Asn Pro Phe Ala 65 70 75 80 ggg tct aat aaa tac gac gga cac
tcc aat aaa tct caa tat gaa atc 288Gly Ser Asn Lys Tyr Asp Gly His
Ser Asn Lys Ser Gln Tyr Glu Ile 85 90 95 att aca caa ggc ggc agc
gga aat caa agc cac gtc acg tat act atc 336Ile Thr Gln Gly Gly Ser
Gly Asn Gln Ser His Val Thr Tyr Thr Ile 100 105 110 cag acc act tca
tcg cgc tac ggg cat aaa tca tag 372Gln Thr Thr Ser Ser Arg Tyr Gly
His Lys Ser 115 120 12123PRTArtificial SequenceSynthetic Construct
12Met Ser Ala Arg Glu Val His Ile Asp Val Asn Asn Lys Thr Gly His 1
5 10 15 Thr Leu Gln Leu Glu Asp Lys Thr Lys Leu Asp Gly Gly Arg Trp
Arg 20 25 30 Thr Ser Pro Thr Asn Val Ala Asn Asp Gln Ile Lys Thr
Phe Val Ala 35 40 45 Glu Ser Asn Gly Phe Met Thr Gly Thr Glu Gly
Thr Ile Tyr Tyr Ser 50 55 60 Ile Asn Gly Glu Ala Glu Ile Ser Leu
Tyr Phe Asp Asn Pro Phe Ala 65 70 75 80 Gly Ser Asn Lys Tyr Asp Gly
His Ser Asn Lys Ser Gln Tyr Glu Ile 85 90 95 Ile Thr Gln Gly Gly
Ser Gly Asn Gln Ser His Val Thr Tyr Thr Ile 100 105 110 Gln Thr Thr
Ser Ser Arg Tyr Gly His Lys Ser 115 120 131152DNABacillus
thuringiensisCDS(1)..(1152)Native DNA sequence encoding Bacillus
thuringiensis Cry35Ab1 toxin 13atg tta gat act aat aaa gtt tat gaa
ata agc aat cat gct aat gga 48Met Leu Asp Thr Asn Lys Val Tyr Glu
Ile Ser Asn His Ala Asn Gly 1 5 10 15 cta tat gca gca act tat tta
agt tta gat gat tca ggt gtt agt tta 96Leu Tyr Ala Ala Thr Tyr Leu
Ser Leu Asp Asp Ser Gly Val Ser Leu 20 25 30 atg aat aaa aat gat
gat gat att gat gat tat aac tta aaa tgg ttt 144Met Asn Lys Asn Asp
Asp Asp Ile Asp Asp Tyr Asn Leu Lys Trp Phe 35 40 45 tta ttt cct
att gat gat gat caa tat att att aca agc tat gca gca 192Leu Phe Pro
Ile Asp Asp Asp Gln Tyr Ile Ile Thr Ser Tyr Ala Ala 50 55 60 aat
aat tgt aaa gtt tgg aat gtt aat aat gat aaa ata aat gtt tcg 240Asn
Asn Cys Lys Val Trp Asn Val Asn Asn Asp Lys Ile Asn Val Ser 65 70
75 80 act tat tct tca aca aat tca ata caa aaa tgg caa ata aaa gct
aat 288Thr Tyr Ser Ser Thr Asn Ser Ile Gln Lys Trp Gln Ile Lys Ala
Asn 85 90 95 ggt tct tca tat gta ata caa agt gat aat gga aaa gtc
tta aca gca 336Gly Ser Ser Tyr Val Ile Gln Ser Asp Asn Gly Lys Val
Leu Thr Ala 100 105 110 gga acc ggt caa gct ctt gga ttg ata cgt tta
act gat gaa tcc tca 384Gly Thr Gly Gln Ala Leu Gly Leu Ile Arg Leu
Thr Asp Glu Ser Ser 115 120 125 aat aat ccc aat caa caa tgg aat tta
act tct gta caa aca att caa 432Asn Asn Pro Asn Gln Gln Trp Asn Leu
Thr Ser Val Gln Thr Ile Gln 130 135 140 ctt cca caa aaa cct ata ata
gat aca aaa tta aaa gat tat ccc aaa 480Leu Pro Gln Lys Pro Ile Ile
Asp Thr Lys Leu Lys Asp Tyr Pro Lys 145 150 155 160 tat tca cca act
gga aat ata gat aat gga aca tct cct caa tta atg 528Tyr Ser Pro Thr
Gly Asn Ile Asp Asn Gly Thr Ser Pro Gln Leu Met 165 170 175 gga tgg
aca tta gta cct tgt att atg gta aat gat cca aat ata gat 576Gly Trp
Thr Leu Val Pro Cys Ile Met Val Asn Asp Pro Asn Ile Asp 180 185 190
aaa aat act caa att aaa act act cca tat tat att tta aaa aaa tat
624Lys Asn Thr Gln Ile Lys Thr Thr Pro Tyr Tyr Ile Leu Lys Lys Tyr
195 200 205 caa tat tgg caa cga gca gta gga agt aat gta gct tta cgt
cca cat 672Gln Tyr Trp Gln Arg Ala Val Gly Ser Asn Val Ala Leu Arg
Pro His 210 215 220 gaa aaa aaa tca tat act tat gaa tgg ggc aca gaa
ata gat caa aaa 720Glu Lys Lys Ser Tyr Thr Tyr Glu Trp Gly Thr Glu
Ile Asp Gln Lys 225 230 235 240 aca aca att ata aat aca tta gga ttt
caa atc aat ata gat tca gga 768Thr Thr Ile Ile Asn Thr Leu Gly Phe
Gln Ile Asn Ile Asp Ser Gly 245 250 255 atg aaa ttt gat ata cca gaa
gta ggt gga ggt aca gat gaa ata aaa 816Met Lys Phe Asp Ile Pro Glu
Val Gly Gly Gly Thr Asp Glu Ile Lys 260 265 270 aca caa cta aat gaa
gaa tta aaa ata gaa tat agt cat gaa act aaa 864Thr Gln Leu Asn Glu
Glu Leu Lys Ile Glu Tyr Ser His Glu Thr Lys 275 280 285 ata atg gaa
aaa tat caa gaa caa tct gaa ata gat aat cca act gat 912Ile Met Glu
Lys Tyr Gln Glu Gln Ser Glu Ile Asp Asn Pro Thr Asp 290 295 300 caa
tca atg aat tct ata gga ttt ctt act att act tcc tta gaa tta 960Gln
Ser Met Asn Ser Ile Gly Phe Leu Thr Ile Thr Ser Leu Glu Leu 305 310
315 320 tat aga tat aat ggc tca gaa att cgt ata atg caa att caa acc
tca 1008Tyr Arg Tyr Asn Gly Ser Glu Ile Arg Ile Met Gln Ile Gln Thr
Ser 325 330 335 gat aat gat act tat aat gtt act tct tat cca aat cat
caa caa gct 1056Asp Asn Asp Thr Tyr Asn Val Thr Ser Tyr Pro Asn His
Gln Gln Ala 340 345 350 tta tta ctt ctt aca aat cat tca tat gaa gaa
gta gaa gaa ata aca 1104Leu Leu Leu Leu Thr Asn His Ser Tyr Glu Glu
Val Glu Glu Ile Thr 355 360 365 aat att cct aaa agt aca cta aaa aaa
tta aaa aaa tat tat ttt taa 1152Asn Ile Pro Lys Ser Thr Leu Lys Lys
Leu Lys Lys Tyr Tyr Phe 370 375 380 14383PRTBacillus thuringiensis
14Met Leu Asp Thr Asn Lys Val Tyr Glu Ile Ser Asn His Ala Asn Gly 1
5 10 15 Leu Tyr Ala Ala Thr Tyr Leu Ser Leu Asp Asp Ser Gly Val Ser
Leu 20 25 30 Met Asn Lys Asn Asp Asp Asp Ile Asp Asp Tyr Asn Leu
Lys Trp Phe 35 40 45 Leu Phe Pro Ile Asp Asp Asp Gln Tyr Ile Ile
Thr Ser Tyr Ala Ala 50 55 60 Asn Asn Cys Lys Val Trp Asn Val Asn
Asn Asp Lys Ile Asn Val Ser 65 70 75 80 Thr Tyr Ser Ser Thr Asn Ser
Ile Gln Lys Trp Gln Ile Lys Ala Asn 85 90 95 Gly Ser Ser Tyr Val
Ile Gln Ser Asp Asn Gly Lys Val Leu Thr Ala 100 105 110 Gly Thr Gly
Gln Ala Leu Gly Leu Ile Arg Leu Thr Asp Glu Ser Ser 115 120 125 Asn
Asn Pro Asn Gln Gln Trp Asn Leu Thr Ser Val Gln Thr Ile Gln 130 135
140 Leu Pro Gln Lys Pro Ile Ile Asp Thr Lys Leu Lys Asp Tyr Pro Lys
145 150 155 160 Tyr Ser Pro Thr Gly Asn Ile Asp Asn Gly Thr Ser Pro
Gln Leu Met 165 170 175 Gly Trp Thr Leu Val Pro Cys Ile Met Val Asn
Asp Pro Asn Ile Asp 180 185 190 Lys Asn Thr Gln Ile Lys Thr Thr Pro
Tyr Tyr Ile Leu Lys Lys Tyr 195 200 205 Gln Tyr Trp Gln Arg Ala Val
Gly Ser Asn Val Ala Leu Arg Pro His 210 215 220 Glu Lys Lys Ser Tyr
Thr Tyr Glu Trp Gly Thr Glu Ile Asp Gln Lys 225 230 235 240 Thr Thr
Ile Ile Asn Thr Leu Gly Phe
Gln Ile Asn Ile Asp Ser Gly 245 250 255 Met Lys Phe Asp Ile Pro Glu
Val Gly Gly Gly Thr Asp Glu Ile Lys 260 265 270 Thr Gln Leu Asn Glu
Glu Leu Lys Ile Glu Tyr Ser His Glu Thr Lys 275 280 285 Ile Met Glu
Lys Tyr Gln Glu Gln Ser Glu Ile Asp Asn Pro Thr Asp 290 295 300 Gln
Ser Met Asn Ser Ile Gly Phe Leu Thr Ile Thr Ser Leu Glu Leu 305 310
315 320 Tyr Arg Tyr Asn Gly Ser Glu Ile Arg Ile Met Gln Ile Gln Thr
Ser 325 330 335 Asp Asn Asp Thr Tyr Asn Val Thr Ser Tyr Pro Asn His
Gln Gln Ala 340 345 350 Leu Leu Leu Leu Thr Asn His Ser Tyr Glu Glu
Val Glu Glu Ile Thr 355 360 365 Asn Ile Pro Lys Ser Thr Leu Lys Lys
Leu Lys Lys Tyr Tyr Phe 370 375 380 151152DNAArtificial
SequenceSynthetic DNA sequence encoding Bacillus thuringiensis
Cry35Ab1 toxin using codons optimized for maize and Table 1
sequences are maintainedCDS(1)..(1152) 15atg ctc gat act aat aaa
gtg tat gaa ata agc aac cat gcc aac ggg 48Met Leu Asp Thr Asn Lys
Val Tyr Glu Ile Ser Asn His Ala Asn Gly1 5 10 15 cta tat gcc gca
act tat ttg agt ctg gac gac agc ggt gtg agc tta 96Leu Tyr Ala Ala
Thr Tyr Leu Ser Leu Asp Asp Ser Gly Val Ser Leu 20 25 30 atg aat
aaa aac gac gac gac att gac gac tac aac ctc aag tgg ttt 144Met Asn
Lys Asn Asp Asp Asp Ile Asp Asp Tyr Asn Leu Lys Trp Phe 35 40 45
tta ttt cct att gac gac gat caa tat att att aca agc tac gca gca
192Leu Phe Pro Ile Asp Asp Asp Gln Tyr Ile Ile Thr Ser Tyr Ala Ala
50 55 60 aat aat tgc aaa gtc tgg aac gtt aat aat gat aaa ata aat
gtt tcg 240Asn Asn Cys Lys Val Trp Asn Val Asn Asn Asp Lys Ile Asn
Val Ser65 70 75 80 acc tac agc tcc acc aac tca ata caa aag tgg caa
ata aaa gct aat 288Thr Tyr Ser Ser Thr Asn Ser Ile Gln Lys Trp Gln
Ile Lys Ala Asn 85 90 95 gga tcg tcg tat gta ata cag agt gac aat
ggg aag gtc ttg aca gcg 336Gly Ser Ser Tyr Val Ile Gln Ser Asp Asn
Gly Lys Val Leu Thr Ala 100 105 110 ggc act ggt caa gct ctt gga ctc
ata agg ctc act gac gag tcc tca 384Gly Thr Gly Gln Ala Leu Gly Leu
Ile Arg Leu Thr Asp Glu Ser Ser 115 120 125 aat aat ccc aat caa cag
tgg aac ttg act tcc gtg cag acg atc caa 432Asn Asn Pro Asn Gln Gln
Trp Asn Leu Thr Ser Val Gln Thr Ile Gln 130 135 140 ctt cca cag aaa
cct atc atc gat aca aaa tta aaa gat tac ccc aag 480Leu Pro Gln Lys
Pro Ile Ile Asp Thr Lys Leu Lys Asp Tyr Pro Lys145 150 155 160tac
tcg cca acc ggc aac atc gat aat gga acg tct cct caa tta atg 528Tyr
Ser Pro Thr Gly Asn Ile Asp Asn Gly Thr Ser Pro Gln Leu Met 165 170
175 ggc tgg acc ctc gta ccc tgt att atg gtg aac gac ccg aat atc gat
576Gly Trp Thr Leu Val Pro Cys Ile Met Val Asn Asp Pro Asn Ile Asp
180 185 190 aaa aat act caa att aaa acc acg ccg tat tat ata ttg aaa
aaa tac 624Lys Asn Thr Gln Ile Lys Thr Thr Pro Tyr Tyr Ile Leu Lys
Lys Tyr 195 200 205 caa tac tgg cag cgc gcg gtt ggc tca aac gtc gct
ctg cgg cca cat 672Gln Tyr Trp Gln Arg Ala Val Gly Ser Asn Val Ala
Leu Arg Pro His 210 215 220 gaa aag aag tcc tac act tac gaa tgg ggc
aca gag atc gat cag aaa 720Glu Lys Lys Ser Tyr Thr Tyr Glu Trp Gly
Thr Glu Ile Asp Gln Lys225 230 235 240acg acc att ata aat aca tta
gga ttc caa atc aat atc gac agc gga 768Thr Thr Ile Ile Asn Thr Leu
Gly Phe Gln Ile Asn Ile Asp Ser Gly 245 250 255 atg aaa ttt gac atc
ccg gaa gtg ggg ggc ggg acc gat gaa ata aaa 816Met Lys Phe Asp Ile
Pro Glu Val Gly Gly Gly Thr Asp Glu Ile Lys 260 265 270 acg cag ctc
aac gaa gaa tta aaa ata gag tac agt cat gaa act aaa 864Thr Gln Leu
Asn Glu Glu Leu Lys Ile Glu Tyr Ser His Glu Thr Lys 275 280 285 ata
atg gaa aaa tat caa gag caa tct gaa atc gat aac ccg acc gac 912Ile
Met Glu Lys Tyr Gln Glu Gln Ser Glu Ile Asp Asn Pro Thr Asp 290 295
300 caa tca atg aac tct atc ggt ttc ctt act att acc tcc ctg gag tta
960Gln Ser Met Asn Ser Ile Gly Phe Leu Thr Ile Thr Ser Leu Glu
Leu305 310 315 320tat aga tat aac ggc tct gag atc cgt ata atg cag
att caa acc tca 1008Tyr Arg Tyr Asn Gly Ser Glu Ile Arg Ile Met Gln
Ile Gln Thr Ser 325 330 335 gac aat gac act tat aac gtc acc tct tac
ccg aat cat caa caa gct 1056Asp Asn Asp Thr Tyr Asn Val Thr Ser Tyr
Pro Asn His Gln Gln Ala 340 345 350 tta ttg ctt ctt aca aac cac agt
tat gaa gag gtg gaa gaa ata acg 1104Leu Leu Leu Leu Thr Asn His Ser
Tyr Glu Glu Val Glu Glu Ile Thr 355 360 365 aac att cct aaa tcc aca
cta aag aaa tta aaa aaa tat tat ttt tga 1152Asn Ile Pro Lys Ser Thr
Leu Lys Lys Leu Lys Lys Tyr Tyr Phe 370 375 380 16383PRTArtificial
SequenceSynthetic Construct 16Met Leu Asp Thr Asn Lys Val Tyr Glu
Ile Ser Asn His Ala Asn Gly 1 5 10 15 Leu Tyr Ala Ala Thr Tyr Leu
Ser Leu Asp Asp Ser Gly Val Ser Leu 20 25 30 Met Asn Lys Asn Asp
Asp Asp Ile Asp Asp Tyr Asn Leu Lys Trp Phe 35 40 45 Leu Phe Pro
Ile Asp Asp Asp Gln Tyr Ile Ile Thr Ser Tyr Ala Ala 50 55 60 Asn
Asn Cys Lys Val Trp Asn Val Asn Asn Asp Lys Ile Asn Val Ser 65 70
75 80 Thr Tyr Ser Ser Thr Asn Ser Ile Gln Lys Trp Gln Ile Lys Ala
Asn 85 90 95 Gly Ser Ser Tyr Val Ile Gln Ser Asp Asn Gly Lys Val
Leu Thr Ala 100 105 110 Gly Thr Gly Gln Ala Leu Gly Leu Ile Arg Leu
Thr Asp Glu Ser Ser 115 120 125 Asn Asn Pro Asn Gln Gln Trp Asn Leu
Thr Ser Val Gln Thr Ile Gln 130 135 140 Leu Pro Gln Lys Pro Ile Ile
Asp Thr Lys Leu Lys Asp Tyr Pro Lys 145 150 155 160 Tyr Ser Pro Thr
Gly Asn Ile Asp Asn Gly Thr Ser Pro Gln Leu Met 165 170 175 Gly Trp
Thr Leu Val Pro Cys Ile Met Val Asn Asp Pro Asn Ile Asp 180 185 190
Lys Asn Thr Gln Ile Lys Thr Thr Pro Tyr Tyr Ile Leu Lys Lys Tyr 195
200 205 Gln Tyr Trp Gln Arg Ala Val Gly Ser Asn Val Ala Leu Arg Pro
His 210 215 220 Glu Lys Lys Ser Tyr Thr Tyr Glu Trp Gly Thr Glu Ile
Asp Gln Lys 225 230 235 240 Thr Thr Ile Ile Asn Thr Leu Gly Phe Gln
Ile Asn Ile Asp Ser Gly 245 250 255 Met Lys Phe Asp Ile Pro Glu Val
Gly Gly Gly Thr Asp Glu Ile Lys 260 265 270 Thr Gln Leu Asn Glu Glu
Leu Lys Ile Glu Tyr Ser His Glu Thr Lys 275 280 285 Ile Met Glu Lys
Tyr Gln Glu Gln Ser Glu Ile Asp Asn Pro Thr Asp 290 295 300 Gln Ser
Met Asn Ser Ile Gly Phe Leu Thr Ile Thr Ser Leu Glu Leu 305 310 315
320 Tyr Arg Tyr Asn Gly Ser Glu Ile Arg Ile Met Gln Ile Gln Thr Ser
325 330 335 Asp Asn Asp Thr Tyr Asn Val Thr Ser Tyr Pro Asn His Gln
Gln Ala 340 345 350 Leu Leu Leu Leu Thr Asn His Ser Tyr Glu Glu Val
Glu Glu Ile Thr 355 360 365 Asn Ile Pro Lys Ser Thr Leu Lys Lys Leu
Lys Lys Tyr Tyr Phe 370 375 380 171152DNAArtificial
SequenceSynthetic DNA sequence in accordance with the invention
encoding Bacillus thuringiensis Cry35Ab1 toxin using codons
optimized for maize and with sequences identified in Table 2
removed and Table 1 sequences are maintainedCDS(1)..(1152) 17atg
ctc gat act aat aaa gtg tat gaa ata tcg aac cat gcc aac ggg 48Met
Leu Asp Thr Asn Lys Val Tyr Glu Ile Ser Asn His Ala Asn Gly1 5 10
15 cta tat gcc gca act tac ctg agt ctg gac gat agt ggt gtg agc tta
96Leu Tyr Ala Ala Thr Tyr Leu Ser Leu Asp Asp Ser Gly Val Ser Leu
20 25 30 atg aat aaa aac gac gac gac att gac gac tac aac ctc aag
tgg ttc 144Met Asn Lys Asn Asp Asp Asp Ile Asp Asp Tyr Asn Leu Lys
Trp Phe 35 40 45 ctg ttt cct att gac gac gat cag tat atc att aca
agc tac gca gcg 192Leu Phe Pro Ile Asp Asp Asp Gln Tyr Ile Ile Thr
Ser Tyr Ala Ala 50 55 60 aat aat tgc aaa gtc tgg aac gtc aat aat
gat aaa atc aat gtt tcg 240Asn Asn Cys Lys Val Trp Asn Val Asn Asn
Asp Lys Ile Asn Val Ser65 70 75 80 acc tac agc tcc acc aac tca ata
caa aag tgg caa atc aaa gct aat 288Thr Tyr Ser Ser Thr Asn Ser Ile
Gln Lys Trp Gln Ile Lys Ala Asn 85 90 95 ggc agc tcg tac gta ata
cag agt gac aat ggg aag gtc ttg aca gcg 336Gly Ser Ser Tyr Val Ile
Gln Ser Asp Asn Gly Lys Val Leu Thr Ala 100 105 110 ggc act ggt caa
gct ctt gga ctc ata agg ctc act gac gag tcc tcg 384Gly Thr Gly Gln
Ala Leu Gly Leu Ile Arg Leu Thr Asp Glu Ser Ser 115 120 125 aat aat
ccc aat caa cag tgg aac ttg act tcc gtg cag acg atc caa 432Asn Asn
Pro Asn Gln Gln Trp Asn Leu Thr Ser Val Gln Thr Ile Gln 130 135 140
ctt cca cag aaa cct atc atc gat aca aaa tta aaa gat tac ccc aag
480Leu Pro Gln Lys Pro Ile Ile Asp Thr Lys Leu Lys Asp Tyr Pro
Lys145 150 155 160tac tcg cca acc ggc aac atc gat aat gga acg tct
cct caa tta atg 528Tyr Ser Pro Thr Gly Asn Ile Asp Asn Gly Thr Ser
Pro Gln Leu Met 165 170 175 ggc tgg acc ctc gta ccc tgt ata atg gtg
aac gac ccg aat atc gat 576Gly Trp Thr Leu Val Pro Cys Ile Met Val
Asn Asp Pro Asn Ile Asp 180 185 190 aaa aat act caa att aaa acc acg
ccg tac tac ata ctc aaa aaa tac 624Lys Asn Thr Gln Ile Lys Thr Thr
Pro Tyr Tyr Ile Leu Lys Lys Tyr 195 200 205 caa tac tgg cag cgc gcg
gtt ggc tca aac gtc gct ctg cgg cca cat 672Gln Tyr Trp Gln Arg Ala
Val Gly Ser Asn Val Ala Leu Arg Pro His 210 215 220 gaa aag aag tcc
tac act tac gaa tgg ggc aca gag atc gat cag aaa 720Glu Lys Lys Ser
Tyr Thr Tyr Glu Trp Gly Thr Glu Ile Asp Gln Lys225 230 235 240acg
acc att ata aat aca tta gga ttc caa atc aat atc gac agc gga 768Thr
Thr Ile Ile Asn Thr Leu Gly Phe Gln Ile Asn Ile Asp Ser Gly 245 250
255 atg aaa ttt gac atc ccg gaa gtg ggg ggc ggg acc gat gaa ata aaa
816Met Lys Phe Asp Ile Pro Glu Val Gly Gly Gly Thr Asp Glu Ile Lys
260 265 270 acg cag ctc aac gaa gaa tta aaa ata gag tac agt cat gaa
act aaa 864Thr Gln Leu Asn Glu Glu Leu Lys Ile Glu Tyr Ser His Glu
Thr Lys 275 280 285 ata atg gaa aaa tat caa gag caa tct gaa atc gat
aac ccg acc gac 912Ile Met Glu Lys Tyr Gln Glu Gln Ser Glu Ile Asp
Asn Pro Thr Asp 290 295 300 caa tca atg aac tct atc ggt ttc ctt act
att acc tcc ctg gag ttg 960Gln Ser Met Asn Ser Ile Gly Phe Leu Thr
Ile Thr Ser Leu Glu Leu305 310 315 320tac aga tat aac ggc tct gag
atc cgt ata atg cag att caa acc tca 1008Tyr Arg Tyr Asn Gly Ser Glu
Ile Arg Ile Met Gln Ile Gln Thr Ser 325 330 335 gac aat gac act tat
aac gtc acc tct tac ccg aat cat cag caa gcc 1056Asp Asn Asp Thr Tyr
Asn Val Thr Ser Tyr Pro Asn His Gln Gln Ala 340 345 350 ctg ctg ctt
ctt aca aac cac agt tat gaa gag gtg gaa gag ata acg 1104Leu Leu Leu
Leu Thr Asn His Ser Tyr Glu Glu Val Glu Glu Ile Thr 355 360 365 aac
att cct aaa tcc aca cta aag aaa tta aaa aaa tat tat ttc tga 1152Asn
Ile Pro Lys Ser Thr Leu Lys Lys Leu Lys Lys Tyr Tyr Phe 370 375 380
18383PRTArtificial SequenceSynthetic Construct 18Met Leu Asp Thr
Asn Lys Val Tyr Glu Ile Ser Asn His Ala Asn Gly 1 5 10 15 Leu Tyr
Ala Ala Thr Tyr Leu Ser Leu Asp Asp Ser Gly Val Ser Leu 20 25 30
Met Asn Lys Asn Asp Asp Asp Ile Asp Asp Tyr Asn Leu Lys Trp Phe 35
40 45 Leu Phe Pro Ile Asp Asp Asp Gln Tyr Ile Ile Thr Ser Tyr Ala
Ala 50 55 60 Asn Asn Cys Lys Val Trp Asn Val Asn Asn Asp Lys Ile
Asn Val Ser 65 70 75 80 Thr Tyr Ser Ser Thr Asn Ser Ile Gln Lys Trp
Gln Ile Lys Ala Asn 85 90 95 Gly Ser Ser Tyr Val Ile Gln Ser Asp
Asn Gly Lys Val Leu Thr Ala 100 105 110 Gly Thr Gly Gln Ala Leu Gly
Leu Ile Arg Leu Thr Asp Glu Ser Ser 115 120 125 Asn Asn Pro Asn Gln
Gln Trp Asn Leu Thr Ser Val Gln Thr Ile Gln 130 135 140 Leu Pro Gln
Lys Pro Ile Ile Asp Thr Lys Leu Lys Asp Tyr Pro Lys 145 150 155 160
Tyr Ser Pro Thr Gly Asn Ile Asp Asn Gly Thr Ser Pro Gln Leu Met 165
170 175 Gly Trp Thr Leu Val Pro Cys Ile Met Val Asn Asp Pro Asn Ile
Asp 180 185 190 Lys Asn Thr Gln Ile Lys Thr Thr Pro Tyr Tyr Ile Leu
Lys Lys Tyr 195 200 205 Gln Tyr Trp Gln Arg Ala Val Gly Ser Asn Val
Ala Leu Arg Pro His 210 215 220 Glu Lys Lys Ser Tyr Thr Tyr Glu Trp
Gly Thr Glu Ile Asp Gln Lys 225 230 235 240 Thr Thr Ile Ile Asn Thr
Leu Gly Phe Gln Ile Asn Ile Asp Ser Gly 245 250 255 Met Lys Phe Asp
Ile Pro Glu Val Gly Gly Gly Thr Asp Glu Ile Lys 260 265 270 Thr Gln
Leu Asn Glu Glu Leu Lys Ile Glu Tyr Ser His Glu Thr Lys 275 280 285
Ile Met Glu Lys Tyr Gln Glu Gln Ser Glu Ile Asp Asn Pro Thr Asp 290
295 300 Gln Ser Met Asn Ser Ile Gly Phe Leu Thr Ile Thr Ser Leu Glu
Leu 305 310 315 320 Tyr Arg Tyr Asn Gly Ser Glu Ile Arg Ile Met Gln
Ile Gln Thr Ser 325 330 335 Asp Asn Asp Thr Tyr Asn Val Thr Ser Tyr
Pro Asn His Gln Gln Ala 340 345 350 Leu Leu Leu Leu Thr Asn His Ser
Tyr Glu Glu Val Glu Glu Ile Thr 355 360 365 Asn Ile Pro Lys Ser Thr
Leu Lys Lys Leu Lys Lys Tyr Tyr Phe 370 375 380 191830DNABacillus
thuringiensisCDS(1)..(1830)Native DNA sequence encoding Bacillus
thuringiensis Cry1Ab1 core toxin 19atg gat aac aat ccg aac atc aat
gaa tgc att cct tat aat tgt tta 48Met Asp Asn Asn Pro Asn Ile Asn
Glu Cys Ile Pro Tyr Asn Cys Leu 1 5 10 15 agt aac cct gaa gta gaa
gta tta ggt gga gaa aga ata gaa act ggt 96Ser Asn Pro Glu Val Glu
Val Leu Gly Gly Glu Arg Ile Glu Thr Gly 20 25 30 tac acc cca atc
gat att tcc ttg tcg cta acg caa ttt ctt ttg agt 144Tyr Thr Pro Ile
Asp Ile Ser Leu Ser Leu Thr Gln Phe Leu Leu Ser 35 40 45 gaa ttt
gtt ccc ggt gct gga ttt gtg tta gga cta gtt gat ata ata 192Glu Phe
Val Pro Gly Ala Gly Phe Val Leu Gly Leu Val Asp Ile Ile 50 55 60
tgg
gga att ttt ggt ccc tct caa tgg gac gca ttt ctt gta caa att 240Trp
Gly Ile Phe Gly Pro Ser Gln Trp Asp Ala Phe Leu Val Gln Ile65 70 75
80 gaa cag tta att aac caa aga ata gaa gaa ttc gct agg aac caa gcc
288Glu Gln Leu Ile Asn Gln Arg Ile Glu Glu Phe Ala Arg Asn Gln Ala
85 90 95 att tct aga tta gaa gga cta agc aat ctt tat caa att tac
gca gaa 336Ile Ser Arg Leu Glu Gly Leu Ser Asn Leu Tyr Gln Ile Tyr
Ala Glu 100 105 110 tct ttt aga gag tgg gaa gca gat cct act aat cca
gca tta aga gaa 384Ser Phe Arg Glu Trp Glu Ala Asp Pro Thr Asn Pro
Ala Leu Arg Glu 115 120 125 gag atg cgt att caa ttc aat gac atg aac
agt gcc ctt aca acc gct 432Glu Met Arg Ile Gln Phe Asn Asp Met Asn
Ser Ala Leu Thr Thr Ala 130 135 140 att cct ctt ttt gca gtt caa aat
tat caa gtt cct ctt tta tca gta 480Ile Pro Leu Phe Ala Val Gln Asn
Tyr Gln Val Pro Leu Leu Ser Val145 150 155 160tat gtt caa gct gca
aat tta cat tta tca gtt ttg aga gat gtt tca 528Tyr Val Gln Ala Ala
Asn Leu His Leu Ser Val Leu Arg Asp Val Ser 165 170 175 gtg ttt gga
caa agg tgg gga ttt gat gcc gcg act atc aat agt cgt 576Val Phe Gly
Gln Arg Trp Gly Phe Asp Ala Ala Thr Ile Asn Ser Arg 180 185 190 tat
aat gat tta act agg ctt att ggc aac tat aca gat tat gct gta 624Tyr
Asn Asp Leu Thr Arg Leu Ile Gly Asn Tyr Thr Asp Tyr Ala Val 195 200
205 cgc tgg tac aat acg gga tta gaa cgt gta tgg gga ccg gat tct aga
672Arg Trp Tyr Asn Thr Gly Leu Glu Arg Val Trp Gly Pro Asp Ser Arg
210 215 220 gat tgg gta agg tat aat caa ttt aga aga gaa tta aca cta
act gta 720Asp Trp Val Arg Tyr Asn Gln Phe Arg Arg Glu Leu Thr Leu
Thr Val225 230 235 240tta gat atc gtt gct ctg ttc ccg aat tat gat
agt aga aga tat cca 768Leu Asp Ile Val Ala Leu Phe Pro Asn Tyr Asp
Ser Arg Arg Tyr Pro 245 250 255 att cga aca gtt tcc caa tta aca aga
gaa att tat aca aac cca gta 816Ile Arg Thr Val Ser Gln Leu Thr Arg
Glu Ile Tyr Thr Asn Pro Val 260 265 270 tta gaa aat ttt gat ggt agt
ttt cga ggc tcg gct cag ggc ata gaa 864Leu Glu Asn Phe Asp Gly Ser
Phe Arg Gly Ser Ala Gln Gly Ile Glu 275 280 285 aga agt att agg agt
cca cat ttg atg gat ata ctt aac agt ata acc 912Arg Ser Ile Arg Ser
Pro His Leu Met Asp Ile Leu Asn Ser Ile Thr 290 295 300 atc tat acg
gat gct cat agg ggt tat tat tat tgg tca ggg cat caa 960Ile Tyr Thr
Asp Ala His Arg Gly Tyr Tyr Tyr Trp Ser Gly His Gln305 310 315
320ata atg gct tct cct gta ggg ttt tcg ggg cca gaa ttc act ttt ccg
1008Ile Met Ala Ser Pro Val Gly Phe Ser Gly Pro Glu Phe Thr Phe Pro
325 330 335 cta tat gga act atg gga aat gca gct cca caa caa cgt att
gtt gct 1056Leu Tyr Gly Thr Met Gly Asn Ala Ala Pro Gln Gln Arg Ile
Val Ala 340 345 350 caa cta ggt cag ggc gtg tat aga aca tta tcg tcc
act tta tat aga 1104Gln Leu Gly Gln Gly Val Tyr Arg Thr Leu Ser Ser
Thr Leu Tyr Arg 355 360 365 aga cct ttt aat ata ggg ata aat aat caa
caa cta tct gtt ctt gac 1152Arg Pro Phe Asn Ile Gly Ile Asn Asn Gln
Gln Leu Ser Val Leu Asp 370 375 380 ggg aca gaa ttt gct tat gga acc
tcc tca aat ttg cca tcc gct gta 1200Gly Thr Glu Phe Ala Tyr Gly Thr
Ser Ser Asn Leu Pro Ser Ala Val385 390 395 400tac aga aaa agc gga
acg gta gat tcg ctg gat gaa ata ccg cca cag 1248Tyr Arg Lys Ser Gly
Thr Val Asp Ser Leu Asp Glu Ile Pro Pro Gln 405 410 415 aat aac aac
gtg cca cct agg caa gga ttt agt cat cga tta agc cat 1296Asn Asn Asn
Val Pro Pro Arg Gln Gly Phe Ser His Arg Leu Ser His 420 425 430 gtt
tca atg ttt cgt tca ggc ttt agt aat agt agt gta agt ata ata 1344Val
Ser Met Phe Arg Ser Gly Phe Ser Asn Ser Ser Val Ser Ile Ile 435 440
445 aga gct cct atg ttc tct tgg ata cat cgt agt gct gaa ttt aat aat
1392Arg Ala Pro Met Phe Ser Trp Ile His Arg Ser Ala Glu Phe Asn Asn
450 455 460 ata att cct tca tca caa att aca caa ata cct tta aca aaa
tct act 1440Ile Ile Pro Ser Ser Gln Ile Thr Gln Ile Pro Leu Thr Lys
Ser Thr465 470 475 480aat ctt ggc tct gga act tct gtc gtt aaa gga
cca gga ttt aca gga 1488Asn Leu Gly Ser Gly Thr Ser Val Val Lys Gly
Pro Gly Phe Thr Gly 485 490 495 gga gat att ctt cga aga act tca cct
ggc cag att tca acc tta aga 1536Gly Asp Ile Leu Arg Arg Thr Ser Pro
Gly Gln Ile Ser Thr Leu Arg 500 505 510 gta aat att act gca cca tta
tca caa aga tat cgg gta aga att cgc 1584Val Asn Ile Thr Ala Pro Leu
Ser Gln Arg Tyr Arg Val Arg Ile Arg 515 520 525 tac gct tct acc aca
aat tta caa ttc cat aca tca att gac gga aga 1632Tyr Ala Ser Thr Thr
Asn Leu Gln Phe His Thr Ser Ile Asp Gly Arg 530 535 540 cct att aat
cag ggg aat ttt tca gca act atg agt agt ggg agt aat 1680Pro Ile Asn
Gln Gly Asn Phe Ser Ala Thr Met Ser Ser Gly Ser Asn545 550 555
560tta cag tcc gga agc ttt agg act gta ggt ttt act act ccg ttt aac
1728Leu Gln Ser Gly Ser Phe Arg Thr Val Gly Phe Thr Thr Pro Phe Asn
565 570 575 ttt tca aat gga tca agt gta ttt acg tta agt gct cat gtc
ttc aat 1776Phe Ser Asn Gly Ser Ser Val Phe Thr Leu Ser Ala His Val
Phe Asn 580 585 590 tca ggc aat gaa gtt tat ata gat cga att gaa ttt
gtt ccg gca gaa 1824Ser Gly Asn Glu Val Tyr Ile Asp Arg Ile Glu Phe
Val Pro Ala Glu 595 600 605 gta acc 1830Val Thr 61020610PRTBacillus
thuringiensis 20Met Asp Asn Asn Pro Asn Ile Asn Glu Cys Ile Pro Tyr
Asn Cys Leu 1 5 10 15 Ser Asn Pro Glu Val Glu Val Leu Gly Gly Glu
Arg Ile Glu Thr Gly 20 25 30 Tyr Thr Pro Ile Asp Ile Ser Leu Ser
Leu Thr Gln Phe Leu Leu Ser 35 40 45 Glu Phe Val Pro Gly Ala Gly
Phe Val Leu Gly Leu Val Asp Ile Ile 50 55 60 Trp Gly Ile Phe Gly
Pro Ser Gln Trp Asp Ala Phe Leu Val Gln Ile 65 70 75 80 Glu Gln Leu
Ile Asn Gln Arg Ile Glu Glu Phe Ala Arg Asn Gln Ala 85 90 95 Ile
Ser Arg Leu Glu Gly Leu Ser Asn Leu Tyr Gln Ile Tyr Ala Glu 100 105
110 Ser Phe Arg Glu Trp Glu Ala Asp Pro Thr Asn Pro Ala Leu Arg Glu
115 120 125 Glu Met Arg Ile Gln Phe Asn Asp Met Asn Ser Ala Leu Thr
Thr Ala 130 135 140 Ile Pro Leu Phe Ala Val Gln Asn Tyr Gln Val Pro
Leu Leu Ser Val 145 150 155 160 Tyr Val Gln Ala Ala Asn Leu His Leu
Ser Val Leu Arg Asp Val Ser 165 170 175 Val Phe Gly Gln Arg Trp Gly
Phe Asp Ala Ala Thr Ile Asn Ser Arg 180 185 190 Tyr Asn Asp Leu Thr
Arg Leu Ile Gly Asn Tyr Thr Asp Tyr Ala Val 195 200 205 Arg Trp Tyr
Asn Thr Gly Leu Glu Arg Val Trp Gly Pro Asp Ser Arg 210 215 220 Asp
Trp Val Arg Tyr Asn Gln Phe Arg Arg Glu Leu Thr Leu Thr Val 225 230
235 240 Leu Asp Ile Val Ala Leu Phe Pro Asn Tyr Asp Ser Arg Arg Tyr
Pro 245 250 255 Ile Arg Thr Val Ser Gln Leu Thr Arg Glu Ile Tyr Thr
Asn Pro Val 260 265 270 Leu Glu Asn Phe Asp Gly Ser Phe Arg Gly Ser
Ala Gln Gly Ile Glu 275 280 285 Arg Ser Ile Arg Ser Pro His Leu Met
Asp Ile Leu Asn Ser Ile Thr 290 295 300 Ile Tyr Thr Asp Ala His Arg
Gly Tyr Tyr Tyr Trp Ser Gly His Gln 305 310 315 320 Ile Met Ala Ser
Pro Val Gly Phe Ser Gly Pro Glu Phe Thr Phe Pro 325 330 335 Leu Tyr
Gly Thr Met Gly Asn Ala Ala Pro Gln Gln Arg Ile Val Ala 340 345 350
Gln Leu Gly Gln Gly Val Tyr Arg Thr Leu Ser Ser Thr Leu Tyr Arg 355
360 365 Arg Pro Phe Asn Ile Gly Ile Asn Asn Gln Gln Leu Ser Val Leu
Asp 370 375 380 Gly Thr Glu Phe Ala Tyr Gly Thr Ser Ser Asn Leu Pro
Ser Ala Val 385 390 395 400 Tyr Arg Lys Ser Gly Thr Val Asp Ser Leu
Asp Glu Ile Pro Pro Gln 405 410 415 Asn Asn Asn Val Pro Pro Arg Gln
Gly Phe Ser His Arg Leu Ser His 420 425 430 Val Ser Met Phe Arg Ser
Gly Phe Ser Asn Ser Ser Val Ser Ile Ile 435 440 445 Arg Ala Pro Met
Phe Ser Trp Ile His Arg Ser Ala Glu Phe Asn Asn 450 455 460 Ile Ile
Pro Ser Ser Gln Ile Thr Gln Ile Pro Leu Thr Lys Ser Thr 465 470 475
480 Asn Leu Gly Ser Gly Thr Ser Val Val Lys Gly Pro Gly Phe Thr Gly
485 490 495 Gly Asp Ile Leu Arg Arg Thr Ser Pro Gly Gln Ile Ser Thr
Leu Arg 500 505 510 Val Asn Ile Thr Ala Pro Leu Ser Gln Arg Tyr Arg
Val Arg Ile Arg 515 520 525 Tyr Ala Ser Thr Thr Asn Leu Gln Phe His
Thr Ser Ile Asp Gly Arg 530 535 540 Pro Ile Asn Gln Gly Asn Phe Ser
Ala Thr Met Ser Ser Gly Ser Asn 545 550 555 560 Leu Gln Ser Gly Ser
Phe Arg Thr Val Gly Phe Thr Thr Pro Phe Asn 565 570 575 Phe Ser Asn
Gly Ser Ser Val Phe Thr Leu Ser Ala His Val Phe Asn 580 585 590 Ser
Gly Asn Glu Val Tyr Ile Asp Arg Ile Glu Phe Val Pro Ala Glu 595 600
605 Val Thr 610 211830DNAArtificial SequenceSynthetic DNA sequence
encoding Bacillus thuringiensis Cry1Ab1 core toxin using codons
optimized for maize and Table 1 sequences are
maintainedCDS(1)..(1830) 21atg gat aac aac ccg aac atc aat gag tgc
atc ccg tat aac tgt ctc 48Met Asp Asn Asn Pro Asn Ile Asn Glu Cys
Ile Pro Tyr Asn Cys Leu1 5 10 15 agt aac cct gaa gtg gag gtc tta
ggt ggc gaa cgc atc gaa act ggt 96Ser Asn Pro Glu Val Glu Val Leu
Gly Gly Glu Arg Ile Glu Thr Gly 20 25 30 tac acc cca atc gac att
agc ttg tcg ttg acg cag ttc ctt ttg tcc 144Tyr Thr Pro Ile Asp Ile
Ser Leu Ser Leu Thr Gln Phe Leu Leu Ser 35 40 45 gag ttc gtg ccc
ggt gcg ggt ttc gtg ctg ggg cta gtt gat ata atc 192Glu Phe Val Pro
Gly Ala Gly Phe Val Leu Gly Leu Val Asp Ile Ile 50 55 60 tgg gga
atc ttt ggt ccc tct cag tgg gac gcc ttt ctt gtg caa att 240Trp Gly
Ile Phe Gly Pro Ser Gln Trp Asp Ala Phe Leu Val Gln Ile65 70 75 80
gag cag cta att aac caa aga ata gaa gag ttc gcg agg aac caa gcc
288Glu Gln Leu Ile Asn Gln Arg Ile Glu Glu Phe Ala Arg Asn Gln Ala
85 90 95 att tcc aga ctg gag gga cta agc aac ctt tat caa atc tac
gcg gag 336Ile Ser Arg Leu Glu Gly Leu Ser Asn Leu Tyr Gln Ile Tyr
Ala Glu 100 105 110 tct ttt agg gag tgg gag gca gat cct acg aac ccg
gca ctg cgc gaa 384Ser Phe Arg Glu Trp Glu Ala Asp Pro Thr Asn Pro
Ala Leu Arg Glu 115 120 125 gag atg cgt att cag ttc aac gac atg aac
agt gcc ctt aca acc gct 432Glu Met Arg Ile Gln Phe Asn Asp Met Asn
Ser Ala Leu Thr Thr Ala 130 135 140 att ccc ctt ttc gca gtt caa aat
tac caa gtt ccc ctt ctc tca gtg 480Ile Pro Leu Phe Ala Val Gln Asn
Tyr Gln Val Pro Leu Leu Ser Val145 150 155 160tac gtt caa gcc gca
aat tta cac cta agc gtt ctc cgc gat gtg tca 528Tyr Val Gln Ala Ala
Asn Leu His Leu Ser Val Leu Arg Asp Val Ser 165 170 175 gtg ttt ggc
cag agg tgg gga ttt gat gcc gcc act atc aat agt cgt 576Val Phe Gly
Gln Arg Trp Gly Phe Asp Ala Ala Thr Ile Asn Ser Arg 180 185 190 tat
aat gat ctg acg agg ctt atc ggc aac tat acc gac tat gct gtc 624Tyr
Asn Asp Leu Thr Arg Leu Ile Gly Asn Tyr Thr Asp Tyr Ala Val 195 200
205 cgc tgg tac aat acg gga tta gag cgg gtc tgg ggt ccg gat tcc cga
672Arg Trp Tyr Asn Thr Gly Leu Glu Arg Val Trp Gly Pro Asp Ser Arg
210 215 220 gac tgg gtg cgc tac aat caa ttc cgc cgc gaa tta acc ctc
act gtc 720Asp Trp Val Arg Tyr Asn Gln Phe Arg Arg Glu Leu Thr Leu
Thr Val225 230 235 240ctc gac atc gtg gcg ctg ttc ccg aac tac gac
agt agg aga tac cca 768Leu Asp Ile Val Ala Leu Phe Pro Asn Tyr Asp
Ser Arg Arg Tyr Pro 245 250 255 atc cgc aca gtt tcc caa tta acg cgg
gaa att tac acc aac cca gtc 816Ile Arg Thr Val Ser Gln Leu Thr Arg
Glu Ile Tyr Thr Asn Pro Val 260 265 270 ctg gag aat ttt gac ggg agc
ttc cga ggc tcg gct caa ggc ata gaa 864Leu Glu Asn Phe Asp Gly Ser
Phe Arg Gly Ser Ala Gln Gly Ile Glu 275 280 285 cgc agc att agg tcg
cca cac ttg atg gat atc ctt aac agc atc acc 912Arg Ser Ile Arg Ser
Pro His Leu Met Asp Ile Leu Asn Ser Ile Thr 290 295 300 atc tac acg
gat gcc cat agg ggt tac tac tac tgg tcg ggg cat caa 960Ile Tyr Thr
Asp Ala His Arg Gly Tyr Tyr Tyr Trp Ser Gly His Gln305 310 315
320ata atg gct tct cct gtc ggg ttt tcg ggg cca gag ttc acc ttc ccg
1008Ile Met Ala Ser Pro Val Gly Phe Ser Gly Pro Glu Phe Thr Phe Pro
325 330 335 ctc tac ggc act atg gga aat gcc gcg cca caa caa cgt atc
gtc gct 1056Leu Tyr Gly Thr Met Gly Asn Ala Ala Pro Gln Gln Arg Ile
Val Ala 340 345 350 caa cta ggt caa ggc gtg tac cgg aca ctg tcg tcc
act ctc tat cgg 1104Gln Leu Gly Gln Gly Val Tyr Arg Thr Leu Ser Ser
Thr Leu Tyr Arg 355 360 365 cgg cct ttc aat ata ggg ata aat aat caa
cag ttg tct gtg ctg gac 1152Arg Pro Phe Asn Ile Gly Ile Asn Asn Gln
Gln Leu Ser Val Leu Asp 370 375 380 ggg aca gag ttt gct tac gga acc
tca agc aac ttg cca tcc gct gta 1200Gly Thr Glu Phe Ala Tyr Gly Thr
Ser Ser Asn Leu Pro Ser Ala Val385 390 395 400tac aga aaa agc ggc
acg gtg gac tcg ctg gat gaa atc ccg ccc cag 1248Tyr Arg Lys Ser Gly
Thr Val Asp Ser Leu Asp Glu Ile Pro Pro Gln 405 410 415 aat aac aac
gtg ccc cct cgg caa ggc ttc agt cat cga ctg agc cac 1296Asn Asn Asn
Val Pro Pro Arg Gln Gly Phe Ser His Arg Leu Ser His 420 425 430 gtt
agc atg ttc cgt tcg ggc ttc agc aac tcc tcc gta agt ata ata 1344Val
Ser Met Phe Arg Ser Gly Phe Ser Asn Ser Ser Val Ser Ile Ile 435 440
445 aga gca cct atg ttc agc tgg ata cat cgt tcc gcc gag ttt aat aat
1392Arg Ala Pro Met Phe Ser Trp Ile His Arg Ser Ala Glu Phe Asn Asn
450 455 460 ata att ccc tcc tct caa atc aca cag atc cct ctg aca aag
tct act 1440Ile Ile Pro Ser Ser Gln Ile Thr Gln Ile Pro Leu Thr Lys
Ser Thr465 470 475 480aat ctt ggc tct ggg act tct gtc gtt aag ggg
cct ggc ttt acg ggc 1488Asn Leu Gly Ser Gly Thr Ser Val Val Lys Gly
Pro Gly Phe Thr Gly 485 490 495 ggc gat att ctg cgg aga act tca cct
ggc cag att tcc acc ctg cgc 1536Gly Asp Ile Leu Arg Arg Thr Ser Pro
Gly Gln Ile Ser Thr Leu Arg 500 505
510 gtg aat atc acc gcg cca ttg tca caa cgt tac cgc gtg cgg att cgc
1584Val Asn Ile Thr Ala Pro Leu Ser Gln Arg Tyr Arg Val Arg Ile Arg
515 520 525 tac gct tct acc aca aac ctc cag ttc cat aca tct att gac
ggc aga 1632Tyr Ala Ser Thr Thr Asn Leu Gln Phe His Thr Ser Ile Asp
Gly Arg 530 535 540 ccc att aat caa ggg aat ttc tcc gcc acg atg tcg
tcc ggc tcc aat 1680Pro Ile Asn Gln Gly Asn Phe Ser Ala Thr Met Ser
Ser Gly Ser Asn545 550 555 560ctc cag tcc gga agt ttc cgc acc gta
ggt ttt act acc ccg ttc aac 1728Leu Gln Ser Gly Ser Phe Arg Thr Val
Gly Phe Thr Thr Pro Phe Asn 565 570 575 ttt tca aac ggc tca agt gtg
ttt acg ctg tcc gct cat gtg ttc aac 1776Phe Ser Asn Gly Ser Ser Val
Phe Thr Leu Ser Ala His Val Phe Asn 580 585 590 tct ggc aat gag gtt
tat atc gac cgg att gag ttc gtc ccg gca gaa 1824Ser Gly Asn Glu Val
Tyr Ile Asp Arg Ile Glu Phe Val Pro Ala Glu 595 600 605 gtc acc
1830Val Thr 61022610PRTArtificial SequenceSynthetic Construct 22Met
Asp Asn Asn Pro Asn Ile Asn Glu Cys Ile Pro Tyr Asn Cys Leu 1 5 10
15 Ser Asn Pro Glu Val Glu Val Leu Gly Gly Glu Arg Ile Glu Thr Gly
20 25 30 Tyr Thr Pro Ile Asp Ile Ser Leu Ser Leu Thr Gln Phe Leu
Leu Ser 35 40 45 Glu Phe Val Pro Gly Ala Gly Phe Val Leu Gly Leu
Val Asp Ile Ile 50 55 60 Trp Gly Ile Phe Gly Pro Ser Gln Trp Asp
Ala Phe Leu Val Gln Ile 65 70 75 80 Glu Gln Leu Ile Asn Gln Arg Ile
Glu Glu Phe Ala Arg Asn Gln Ala 85 90 95 Ile Ser Arg Leu Glu Gly
Leu Ser Asn Leu Tyr Gln Ile Tyr Ala Glu 100 105 110 Ser Phe Arg Glu
Trp Glu Ala Asp Pro Thr Asn Pro Ala Leu Arg Glu 115 120 125 Glu Met
Arg Ile Gln Phe Asn Asp Met Asn Ser Ala Leu Thr Thr Ala 130 135 140
Ile Pro Leu Phe Ala Val Gln Asn Tyr Gln Val Pro Leu Leu Ser Val 145
150 155 160 Tyr Val Gln Ala Ala Asn Leu His Leu Ser Val Leu Arg Asp
Val Ser 165 170 175 Val Phe Gly Gln Arg Trp Gly Phe Asp Ala Ala Thr
Ile Asn Ser Arg 180 185 190 Tyr Asn Asp Leu Thr Arg Leu Ile Gly Asn
Tyr Thr Asp Tyr Ala Val 195 200 205 Arg Trp Tyr Asn Thr Gly Leu Glu
Arg Val Trp Gly Pro Asp Ser Arg 210 215 220 Asp Trp Val Arg Tyr Asn
Gln Phe Arg Arg Glu Leu Thr Leu Thr Val 225 230 235 240 Leu Asp Ile
Val Ala Leu Phe Pro Asn Tyr Asp Ser Arg Arg Tyr Pro 245 250 255 Ile
Arg Thr Val Ser Gln Leu Thr Arg Glu Ile Tyr Thr Asn Pro Val 260 265
270 Leu Glu Asn Phe Asp Gly Ser Phe Arg Gly Ser Ala Gln Gly Ile Glu
275 280 285 Arg Ser Ile Arg Ser Pro His Leu Met Asp Ile Leu Asn Ser
Ile Thr 290 295 300 Ile Tyr Thr Asp Ala His Arg Gly Tyr Tyr Tyr Trp
Ser Gly His Gln 305 310 315 320 Ile Met Ala Ser Pro Val Gly Phe Ser
Gly Pro Glu Phe Thr Phe Pro 325 330 335 Leu Tyr Gly Thr Met Gly Asn
Ala Ala Pro Gln Gln Arg Ile Val Ala 340 345 350 Gln Leu Gly Gln Gly
Val Tyr Arg Thr Leu Ser Ser Thr Leu Tyr Arg 355 360 365 Arg Pro Phe
Asn Ile Gly Ile Asn Asn Gln Gln Leu Ser Val Leu Asp 370 375 380 Gly
Thr Glu Phe Ala Tyr Gly Thr Ser Ser Asn Leu Pro Ser Ala Val 385 390
395 400 Tyr Arg Lys Ser Gly Thr Val Asp Ser Leu Asp Glu Ile Pro Pro
Gln 405 410 415 Asn Asn Asn Val Pro Pro Arg Gln Gly Phe Ser His Arg
Leu Ser His 420 425 430 Val Ser Met Phe Arg Ser Gly Phe Ser Asn Ser
Ser Val Ser Ile Ile 435 440 445 Arg Ala Pro Met Phe Ser Trp Ile His
Arg Ser Ala Glu Phe Asn Asn 450 455 460 Ile Ile Pro Ser Ser Gln Ile
Thr Gln Ile Pro Leu Thr Lys Ser Thr 465 470 475 480 Asn Leu Gly Ser
Gly Thr Ser Val Val Lys Gly Pro Gly Phe Thr Gly 485 490 495 Gly Asp
Ile Leu Arg Arg Thr Ser Pro Gly Gln Ile Ser Thr Leu Arg 500 505 510
Val Asn Ile Thr Ala Pro Leu Ser Gln Arg Tyr Arg Val Arg Ile Arg 515
520 525 Tyr Ala Ser Thr Thr Asn Leu Gln Phe His Thr Ser Ile Asp Gly
Arg 530 535 540 Pro Ile Asn Gln Gly Asn Phe Ser Ala Thr Met Ser Ser
Gly Ser Asn 545 550 555 560 Leu Gln Ser Gly Ser Phe Arg Thr Val Gly
Phe Thr Thr Pro Phe Asn 565 570 575 Phe Ser Asn Gly Ser Ser Val Phe
Thr Leu Ser Ala His Val Phe Asn 580 585 590 Ser Gly Asn Glu Val Tyr
Ile Asp Arg Ile Glu Phe Val Pro Ala Glu 595 600 605 Val Thr 610
231830DNAArtificial SequenceSynthetic DNA sequence in accordance
with the invention encoding Bacillus thuringiensis Cry1Ab1 core
toxin using codons optimized for maize and with sequences
identified in Table 2 removed and Table 1 sequences are
maintainedCDS(1)..(1830) 23atg gat aac aac ccg aac atc aat gag tgc
atc ccg tat aac tgt ctc 48Met Asp Asn Asn Pro Asn Ile Asn Glu Cys
Ile Pro Tyr Asn Cys Leu 1 5 10 15 agt aac cct gaa gtg gag gtc tta
ggt ggc gaa cgc atc gaa act ggt 96Ser Asn Pro Glu Val Glu Val Leu
Gly Gly Glu Arg Ile Glu Thr Gly 20 25 30 tac acc cca atc gac att
agc ttg tcg ttg acg cag ttc ctc ttg tcc 144Tyr Thr Pro Ile Asp Ile
Ser Leu Ser Leu Thr Gln Phe Leu Leu Ser 35 40 45 gag ttc gtg ccc
ggt gcg ggt ttc gtg ctg ggg cta gtt gat ata atc 192Glu Phe Val Pro
Gly Ala Gly Phe Val Leu Gly Leu Val Asp Ile Ile 50 55 60 tgg gga
atc ttt ggt ccc tct cag tgg gac gcc ttt ctt gtg caa att 240Trp Gly
Ile Phe Gly Pro Ser Gln Trp Asp Ala Phe Leu Val Gln Ile 65 70 75 80
gag cag cta att aac caa aga ata gaa gag ttc gcg agg aac caa gcc
288Glu Gln Leu Ile Asn Gln Arg Ile Glu Glu Phe Ala Arg Asn Gln Ala
85 90 95 att tcc aga ctg gag gga cta agc aac ctt tat caa atc tac
gcg gag 336Ile Ser Arg Leu Glu Gly Leu Ser Asn Leu Tyr Gln Ile Tyr
Ala Glu 100 105 110 tct ttt agg gag tgg gag gca gat cct acg aac ccg
gca ctg cgc gaa 384Ser Phe Arg Glu Trp Glu Ala Asp Pro Thr Asn Pro
Ala Leu Arg Glu 115 120 125 gag atg cgt att cag ttc aac gac atg aac
agt gcc ctt aca acc gct 432Glu Met Arg Ile Gln Phe Asn Asp Met Asn
Ser Ala Leu Thr Thr Ala 130 135 140 att ccc ctt ttc gca gtt caa aat
tac caa gtt ccc ctt ctc tca gtg 480Ile Pro Leu Phe Ala Val Gln Asn
Tyr Gln Val Pro Leu Leu Ser Val 145 150 155 160 tac gtt caa gcc gca
aat tta cac cta agc gtt ctc cgc gat gtg tca 528Tyr Val Gln Ala Ala
Asn Leu His Leu Ser Val Leu Arg Asp Val Ser 165 170 175 gtg ttc ggc
cag agg tgg gga ttt gat gcc gcc act atc aat agt cgt 576Val Phe Gly
Gln Arg Trp Gly Phe Asp Ala Ala Thr Ile Asn Ser Arg 180 185 190 tat
aat gat ctg acg agg ctt atc ggc aac tat acc gac tat gct gtc 624Tyr
Asn Asp Leu Thr Arg Leu Ile Gly Asn Tyr Thr Asp Tyr Ala Val 195 200
205 cgc tgg tac aat acg gga tta gag cgg gtc tgg ggt ccg gat tcc cga
672Arg Trp Tyr Asn Thr Gly Leu Glu Arg Val Trp Gly Pro Asp Ser Arg
210 215 220 gac tgg gtg cgc tac aat caa ttc cgc cgc gaa tta acc ctc
act gtc 720Asp Trp Val Arg Tyr Asn Gln Phe Arg Arg Glu Leu Thr Leu
Thr Val 225 230 235 240 ctc gac atc gtg gcg ctg ttc ccg aac tac gac
agt agg aga tac cca 768Leu Asp Ile Val Ala Leu Phe Pro Asn Tyr Asp
Ser Arg Arg Tyr Pro 245 250 255 atc cgc aca gtt tcc caa tta acg cgg
gaa att tac acc aac cca gtc 816Ile Arg Thr Val Ser Gln Leu Thr Arg
Glu Ile Tyr Thr Asn Pro Val 260 265 270 ctg gag aat ttt gac ggg agc
ttc cga ggc tcg gct caa ggc ata gaa 864Leu Glu Asn Phe Asp Gly Ser
Phe Arg Gly Ser Ala Gln Gly Ile Glu 275 280 285 cgc agc att agg tcg
cca cac ttg atg gat atc ctt aac agc atc acc 912Arg Ser Ile Arg Ser
Pro His Leu Met Asp Ile Leu Asn Ser Ile Thr 290 295 300 atc tac acg
gat gcc cat agg ggt tac tac tac tgg tcg ggg cat caa 960Ile Tyr Thr
Asp Ala His Arg Gly Tyr Tyr Tyr Trp Ser Gly His Gln 305 310 315 320
ata atg gct tct cct gtc ggg ttt tcg ggg cca gag ttc acc ttc ccg
1008Ile Met Ala Ser Pro Val Gly Phe Ser Gly Pro Glu Phe Thr Phe Pro
325 330 335 ctc tac ggc act atg gga aat gcc gcg cca caa caa cgt atc
gtc gct 1056Leu Tyr Gly Thr Met Gly Asn Ala Ala Pro Gln Gln Arg Ile
Val Ala 340 345 350 caa cta ggt caa ggc gtg tac cgg aca ctg tcg tcc
act ctc tat cgg 1104Gln Leu Gly Gln Gly Val Tyr Arg Thr Leu Ser Ser
Thr Leu Tyr Arg 355 360 365 cgg cct ttc aat ata ggg atc aat aat caa
cag ttg tct gtg ctg gac 1152Arg Pro Phe Asn Ile Gly Ile Asn Asn Gln
Gln Leu Ser Val Leu Asp 370 375 380 ggg aca gag ttt gct tac gga acc
tca agc aac ttg cca tcc gct gta 1200Gly Thr Glu Phe Ala Tyr Gly Thr
Ser Ser Asn Leu Pro Ser Ala Val 385 390 395 400 tac aga aaa agc ggc
acg gtg gac tcg ctg gat gaa atc ccg ccc cag 1248Tyr Arg Lys Ser Gly
Thr Val Asp Ser Leu Asp Glu Ile Pro Pro Gln 405 410 415 aat aac aac
gtg ccc cct cgg caa ggc ttc agt cat cga ctg agc cac 1296Asn Asn Asn
Val Pro Pro Arg Gln Gly Phe Ser His Arg Leu Ser His 420 425 430 gtt
agc atg ttc cgt tcg ggc ttc agc aac tcc tcc gta agt atc ata 1344Val
Ser Met Phe Arg Ser Gly Phe Ser Asn Ser Ser Val Ser Ile Ile 435 440
445 aga gca cct atg ttc agc tgg ata cat cgt tcc gcc gag ttc aat aat
1392Arg Ala Pro Met Phe Ser Trp Ile His Arg Ser Ala Glu Phe Asn Asn
450 455 460 ata att ccc tcc tct caa atc aca cag atc cct ctg aca aag
tct act 1440Ile Ile Pro Ser Ser Gln Ile Thr Gln Ile Pro Leu Thr Lys
Ser Thr 465 470 475 480 aat ctt ggc tct ggg act tct gtc gtt aag ggg
cct ggc ttt acg ggc 1488Asn Leu Gly Ser Gly Thr Ser Val Val Lys Gly
Pro Gly Phe Thr Gly 485 490 495 ggc gat att ctg cgg aga act tca cct
ggc cag att tcc acc ctg cgc 1536Gly Asp Ile Leu Arg Arg Thr Ser Pro
Gly Gln Ile Ser Thr Leu Arg 500 505 510 gtg aat atc acc gcg cca ttg
tca caa cgt tac cgc gtg cgg att cgc 1584Val Asn Ile Thr Ala Pro Leu
Ser Gln Arg Tyr Arg Val Arg Ile Arg 515 520 525 tac gct tct acc aca
aac ctc cag ttc cat aca tct att gac ggc aga 1632Tyr Ala Ser Thr Thr
Asn Leu Gln Phe His Thr Ser Ile Asp Gly Arg 530 535 540 ccc att aat
caa ggg aat ttc tcc gcc acg atg tcg tcc ggc tcc aat 1680Pro Ile Asn
Gln Gly Asn Phe Ser Ala Thr Met Ser Ser Gly Ser Asn 545 550 555 560
ctc cag tcc gga agt ttc cgc acc gta ggt ttt act acc ccg ttc aac
1728Leu Gln Ser Gly Ser Phe Arg Thr Val Gly Phe Thr Thr Pro Phe Asn
565 570 575 ttt tca aac ggc tca agt gtg ttt acg ctg tcc gct cat gtg
ttc aac 1776Phe Ser Asn Gly Ser Ser Val Phe Thr Leu Ser Ala His Val
Phe Asn 580 585 590 tct ggc aat gag gtt tac atc gac cgg att gag ttc
gtc ccg gca gaa 1824Ser Gly Asn Glu Val Tyr Ile Asp Arg Ile Glu Phe
Val Pro Ala Glu 595 600 605 gtc acc 1830Val Thr 610
24610PRTArtificial SequenceSynthetic Construct 24Met Asp Asn Asn
Pro Asn Ile Asn Glu Cys Ile Pro Tyr Asn Cys Leu 1 5 10 15 Ser Asn
Pro Glu Val Glu Val Leu Gly Gly Glu Arg Ile Glu Thr Gly 20 25 30
Tyr Thr Pro Ile Asp Ile Ser Leu Ser Leu Thr Gln Phe Leu Leu Ser 35
40 45 Glu Phe Val Pro Gly Ala Gly Phe Val Leu Gly Leu Val Asp Ile
Ile 50 55 60 Trp Gly Ile Phe Gly Pro Ser Gln Trp Asp Ala Phe Leu
Val Gln Ile 65 70 75 80 Glu Gln Leu Ile Asn Gln Arg Ile Glu Glu Phe
Ala Arg Asn Gln Ala 85 90 95 Ile Ser Arg Leu Glu Gly Leu Ser Asn
Leu Tyr Gln Ile Tyr Ala Glu 100 105 110 Ser Phe Arg Glu Trp Glu Ala
Asp Pro Thr Asn Pro Ala Leu Arg Glu 115 120 125 Glu Met Arg Ile Gln
Phe Asn Asp Met Asn Ser Ala Leu Thr Thr Ala 130 135 140 Ile Pro Leu
Phe Ala Val Gln Asn Tyr Gln Val Pro Leu Leu Ser Val 145 150 155 160
Tyr Val Gln Ala Ala Asn Leu His Leu Ser Val Leu Arg Asp Val Ser 165
170 175 Val Phe Gly Gln Arg Trp Gly Phe Asp Ala Ala Thr Ile Asn Ser
Arg 180 185 190 Tyr Asn Asp Leu Thr Arg Leu Ile Gly Asn Tyr Thr Asp
Tyr Ala Val 195 200 205 Arg Trp Tyr Asn Thr Gly Leu Glu Arg Val Trp
Gly Pro Asp Ser Arg 210 215 220 Asp Trp Val Arg Tyr Asn Gln Phe Arg
Arg Glu Leu Thr Leu Thr Val 225 230 235 240 Leu Asp Ile Val Ala Leu
Phe Pro Asn Tyr Asp Ser Arg Arg Tyr Pro 245 250 255 Ile Arg Thr Val
Ser Gln Leu Thr Arg Glu Ile Tyr Thr Asn Pro Val 260 265 270 Leu Glu
Asn Phe Asp Gly Ser Phe Arg Gly Ser Ala Gln Gly Ile Glu 275 280 285
Arg Ser Ile Arg Ser Pro His Leu Met Asp Ile Leu Asn Ser Ile Thr 290
295 300 Ile Tyr Thr Asp Ala His Arg Gly Tyr Tyr Tyr Trp Ser Gly His
Gln 305 310 315 320 Ile Met Ala Ser Pro Val Gly Phe Ser Gly Pro Glu
Phe Thr Phe Pro 325 330 335 Leu Tyr Gly Thr Met Gly Asn Ala Ala Pro
Gln Gln Arg Ile Val Ala 340 345 350 Gln Leu Gly Gln Gly Val Tyr Arg
Thr Leu Ser Ser Thr Leu Tyr Arg 355 360 365 Arg Pro Phe Asn Ile Gly
Ile Asn Asn Gln Gln Leu Ser Val Leu Asp 370 375 380 Gly Thr Glu Phe
Ala Tyr Gly Thr Ser Ser Asn Leu Pro Ser Ala Val 385 390 395 400 Tyr
Arg Lys Ser Gly Thr Val Asp Ser Leu Asp Glu Ile Pro Pro Gln 405 410
415 Asn Asn Asn Val Pro Pro Arg Gln Gly Phe Ser His Arg Leu Ser His
420 425 430 Val Ser Met Phe Arg Ser Gly Phe Ser Asn Ser Ser Val Ser
Ile Ile 435 440 445 Arg Ala Pro Met Phe Ser Trp Ile His Arg Ser Ala
Glu Phe Asn Asn 450
455 460 Ile Ile Pro Ser Ser Gln Ile Thr Gln Ile Pro Leu Thr Lys Ser
Thr 465 470 475 480 Asn Leu Gly Ser Gly Thr Ser Val Val Lys Gly Pro
Gly Phe Thr Gly 485 490 495 Gly Asp Ile Leu Arg Arg Thr Ser Pro Gly
Gln Ile Ser Thr Leu Arg 500 505 510 Val Asn Ile Thr Ala Pro Leu Ser
Gln Arg Tyr Arg Val Arg Ile Arg 515 520 525 Tyr Ala Ser Thr Thr Asn
Leu Gln Phe His Thr Ser Ile Asp Gly Arg 530 535 540 Pro Ile Asn Gln
Gly Asn Phe Ser Ala Thr Met Ser Ser Gly Ser Asn 545 550 555 560 Leu
Gln Ser Gly Ser Phe Arg Thr Val Gly Phe Thr Thr Pro Phe Asn 565 570
575 Phe Ser Asn Gly Ser Ser Val Phe Thr Leu Ser Ala His Val Phe Asn
580 585 590 Ser Gly Asn Glu Val Tyr Ile Asp Arg Ile Glu Phe Val Pro
Ala Glu 595 600 605 Val Thr 610 251866DNABacillus
thuringiensisCDS(1)..(1866)Native DNA sequence encoding Bacillus
thuringiensis Cry1Ca core toxin 25atg gat aac aat ccg aac atc aat
gaa tgc atc ccg tac aac tgc ctg 48Met Asp Asn Asn Pro Asn Ile Asn
Glu Cys Ile Pro Tyr Asn Cys Leu1 5 10 15 agc aac ccg gaa gaa gtg
ctg ttg gat gga gaa cgg ata tca act ggt 96Ser Asn Pro Glu Glu Val
Leu Leu Asp Gly Glu Arg Ile Ser Thr Gly 20 25 30 aat tca tca att
gat att tct ctg tca ctt gtt cag ttt ctg gta tct 144Asn Ser Ser Ile
Asp Ile Ser Leu Ser Leu Val Gln Phe Leu Val Ser 35 40 45 aac ttc
gtc cca ggc gga gga ttc ctg gtt gga tta ata gat ttt gta 192Asn Phe
Val Pro Gly Gly Gly Phe Leu Val Gly Leu Ile Asp Phe Val 50 55 60
tgg gga ata gtt ggc cct tct caa tgg gat gca ttt cta gta caa att
240Trp Gly Ile Val Gly Pro Ser Gln Trp Asp Ala Phe Leu Val Gln Ile
65 70 75 80 gaa caa tta att aat gaa aga ata gct gaa ttt gct agg aat
gct gct 288Glu Gln Leu Ile Asn Glu Arg Ile Ala Glu Phe Ala Arg Asn
Ala Ala 85 90 95 att gct aat tta gaa gga tta gga aac aat ttc aat
ata tat gtg gaa 336Ile Ala Asn Leu Glu Gly Leu Gly Asn Asn Phe Asn
Ile Tyr Val Glu 100 105 110 gca ttt aaa gaa tgg gaa gaa gat cct aag
aat cca gca acc agg acc 384Ala Phe Lys Glu Trp Glu Glu Asp Pro Lys
Asn Pro Ala Thr Arg Thr 115 120 125 aga gta att gat cgc ttt cgt ata
ctt gat ggg cta ctt gaa agg gac 432Arg Val Ile Asp Arg Phe Arg Ile
Leu Asp Gly Leu Leu Glu Arg Asp 130 135 140 att cct tcg ttt cga att
tct gga ttt gaa gta ccc ctt tta tcc gtt 480Ile Pro Ser Phe Arg Ile
Ser Gly Phe Glu Val Pro Leu Leu Ser Val 145 150 155 160 tat gct caa
gcg gcc aat ctg cat cta gct ata tta aga gat tct gta 528Tyr Ala Gln
Ala Ala Asn Leu His Leu Ala Ile Leu Arg Asp Ser Val 165 170 175 att
ttt gga gaa aga tgg gga ttg aca acg ata aat gtc aat gaa aac 576Ile
Phe Gly Glu Arg Trp Gly Leu Thr Thr Ile Asn Val Asn Glu Asn 180 185
190 tat aat aga cta att agg cat att gat gaa tat gct gat cac tgt gca
624Tyr Asn Arg Leu Ile Arg His Ile Asp Glu Tyr Ala Asp His Cys Ala
195 200 205 aat acg tat aat cgg gga tta aat aat tta ccg aaa tct acg
tat caa 672Asn Thr Tyr Asn Arg Gly Leu Asn Asn Leu Pro Lys Ser Thr
Tyr Gln 210 215 220 gat tgg ata aca tat aat cga ttg cgg aga gac tta
aca ttg act gta 720Asp Trp Ile Thr Tyr Asn Arg Leu Arg Arg Asp Leu
Thr Leu Thr Val 225 230 235 240 tta gat atc gcc gct ttc ttt cca aac
tat gac aat agg aga tat cca 768Leu Asp Ile Ala Ala Phe Phe Pro Asn
Tyr Asp Asn Arg Arg Tyr Pro 245 250 255 att cag cca gtt ggt caa cta
aca agg gaa gtt tat acg gac cca tta 816Ile Gln Pro Val Gly Gln Leu
Thr Arg Glu Val Tyr Thr Asp Pro Leu 260 265 270 att aat ttt aat cca
cag tta cag tct gta gct caa tta cct act ttt 864Ile Asn Phe Asn Pro
Gln Leu Gln Ser Val Ala Gln Leu Pro Thr Phe 275 280 285 aac gtt atg
gag aac agc gca att aga aat cct cat tta ttt gat ata 912Asn Val Met
Glu Asn Ser Ala Ile Arg Asn Pro His Leu Phe Asp Ile 290 295 300 ttg
aat aat ctt aca atc ttt acg gat tgg ttt agt gtt gga cgc aat 960Leu
Asn Asn Leu Thr Ile Phe Thr Asp Trp Phe Ser Val Gly Arg Asn 305 310
315 320 ttt tat tgg gga gga cat cga gta ata tct agc ctt ata gga ggt
ggt 1008Phe Tyr Trp Gly Gly His Arg Val Ile Ser Ser Leu Ile Gly Gly
Gly 325 330 335 aac ata aca tct cct ata tat gga aga gag gcg aac cag
gag cct cca 1056Asn Ile Thr Ser Pro Ile Tyr Gly Arg Glu Ala Asn Gln
Glu Pro Pro 340 345 350 aga tcc ttt act ttt aat gga ccg gta ttt agg
act tta tca aat cct 1104Arg Ser Phe Thr Phe Asn Gly Pro Val Phe Arg
Thr Leu Ser Asn Pro 355 360 365 act tta cga tta tta cag caa cct tgg
cca gcg cca cca ttt aat tta 1152Thr Leu Arg Leu Leu Gln Gln Pro Trp
Pro Ala Pro Pro Phe Asn Leu 370 375 380 cgt ggt gtt gaa gga gta gaa
ttt tct aca cct aca aat agc ttt acg 1200Arg Gly Val Glu Gly Val Glu
Phe Ser Thr Pro Thr Asn Ser Phe Thr 385 390 395 400 tat cga gga aga
ggt acg gtt gat tct tta act gaa ttg ccg cct gag 1248Tyr Arg Gly Arg
Gly Thr Val Asp Ser Leu Thr Glu Leu Pro Pro Glu 405 410 415 gat aat
agt gtg cca cct cgc gaa gga tat agt cat cgt tta tgt cat 1296Asp Asn
Ser Val Pro Pro Arg Glu Gly Tyr Ser His Arg Leu Cys His 420 425 430
gca act ttt gtt caa aga tct gga aca cct ttt tta aca act ggt gta
1344Ala Thr Phe Val Gln Arg Ser Gly Thr Pro Phe Leu Thr Thr Gly Val
435 440 445 gta ttt tct tgg acg cat cgt agt gca act ctt aca aat aca
att gat 1392Val Phe Ser Trp Thr His Arg Ser Ala Thr Leu Thr Asn Thr
Ile Asp 450 455 460 cca gag aga att aat caa ata cct tta gtg aaa gga
ttt aga gtt tgg 1440Pro Glu Arg Ile Asn Gln Ile Pro Leu Val Lys Gly
Phe Arg Val Trp 465 470 475 480 ggg ggc acc tct gtc att aca gga cca
gga ttt aca gga ggg gat atc 1488Gly Gly Thr Ser Val Ile Thr Gly Pro
Gly Phe Thr Gly Gly Asp Ile 485 490 495 ctt cga aga aat acc ttt ggt
gat ttt gta tct cta caa gtc aat att 1536Leu Arg Arg Asn Thr Phe Gly
Asp Phe Val Ser Leu Gln Val Asn Ile 500 505 510 aat tca cca att acc
caa aga tac cgt tta aga ttt cgt tac gct tcc 1584Asn Ser Pro Ile Thr
Gln Arg Tyr Arg Leu Arg Phe Arg Tyr Ala Ser 515 520 525 agt agg gat
gca cga gtt ata gta tta aca gga gcg gca tcc aca gga 1632Ser Arg Asp
Ala Arg Val Ile Val Leu Thr Gly Ala Ala Ser Thr Gly 530 535 540 gtg
gga ggc caa gtt agt gta aat atg cct ctt cag aaa act atg gaa 1680Val
Gly Gly Gln Val Ser Val Asn Met Pro Leu Gln Lys Thr Met Glu 545 550
555 560 ata ggg gag aac tta aca tct aga aca ttt aga tat acc gat ttt
agt 1728Ile Gly Glu Asn Leu Thr Ser Arg Thr Phe Arg Tyr Thr Asp Phe
Ser 565 570 575 aat cct ttt tca ttt aga gct aat cca gat ata att ggg
ata agt gaa 1776Asn Pro Phe Ser Phe Arg Ala Asn Pro Asp Ile Ile Gly
Ile Ser Glu 580 585 590 caa cct cta ttt ggt gca ggt tct att agt agc
ggt gaa ctt tat ata 1824Gln Pro Leu Phe Gly Ala Gly Ser Ile Ser Ser
Gly Glu Leu Tyr Ile 595 600 605 gat aaa att gaa att att cta gca gat
gca aca ttt gaa taa 1866Asp Lys Ile Glu Ile Ile Leu Ala Asp Ala Thr
Phe Glu 610 615 620 26621PRTBacillus thuringiensis 26Met Asp Asn
Asn Pro Asn Ile Asn Glu Cys Ile Pro Tyr Asn Cys Leu 1 5 10 15 Ser
Asn Pro Glu Glu Val Leu Leu Asp Gly Glu Arg Ile Ser Thr Gly 20 25
30 Asn Ser Ser Ile Asp Ile Ser Leu Ser Leu Val Gln Phe Leu Val Ser
35 40 45 Asn Phe Val Pro Gly Gly Gly Phe Leu Val Gly Leu Ile Asp
Phe Val 50 55 60 Trp Gly Ile Val Gly Pro Ser Gln Trp Asp Ala Phe
Leu Val Gln Ile 65 70 75 80 Glu Gln Leu Ile Asn Glu Arg Ile Ala Glu
Phe Ala Arg Asn Ala Ala 85 90 95 Ile Ala Asn Leu Glu Gly Leu Gly
Asn Asn Phe Asn Ile Tyr Val Glu 100 105 110 Ala Phe Lys Glu Trp Glu
Glu Asp Pro Lys Asn Pro Ala Thr Arg Thr 115 120 125 Arg Val Ile Asp
Arg Phe Arg Ile Leu Asp Gly Leu Leu Glu Arg Asp 130 135 140 Ile Pro
Ser Phe Arg Ile Ser Gly Phe Glu Val Pro Leu Leu Ser Val 145 150 155
160 Tyr Ala Gln Ala Ala Asn Leu His Leu Ala Ile Leu Arg Asp Ser Val
165 170 175 Ile Phe Gly Glu Arg Trp Gly Leu Thr Thr Ile Asn Val Asn
Glu Asn 180 185 190 Tyr Asn Arg Leu Ile Arg His Ile Asp Glu Tyr Ala
Asp His Cys Ala 195 200 205 Asn Thr Tyr Asn Arg Gly Leu Asn Asn Leu
Pro Lys Ser Thr Tyr Gln 210 215 220 Asp Trp Ile Thr Tyr Asn Arg Leu
Arg Arg Asp Leu Thr Leu Thr Val 225 230 235 240 Leu Asp Ile Ala Ala
Phe Phe Pro Asn Tyr Asp Asn Arg Arg Tyr Pro 245 250 255 Ile Gln Pro
Val Gly Gln Leu Thr Arg Glu Val Tyr Thr Asp Pro Leu 260 265 270 Ile
Asn Phe Asn Pro Gln Leu Gln Ser Val Ala Gln Leu Pro Thr Phe 275 280
285 Asn Val Met Glu Asn Ser Ala Ile Arg Asn Pro His Leu Phe Asp Ile
290 295 300 Leu Asn Asn Leu Thr Ile Phe Thr Asp Trp Phe Ser Val Gly
Arg Asn 305 310 315 320 Phe Tyr Trp Gly Gly His Arg Val Ile Ser Ser
Leu Ile Gly Gly Gly 325 330 335 Asn Ile Thr Ser Pro Ile Tyr Gly Arg
Glu Ala Asn Gln Glu Pro Pro 340 345 350 Arg Ser Phe Thr Phe Asn Gly
Pro Val Phe Arg Thr Leu Ser Asn Pro 355 360 365 Thr Leu Arg Leu Leu
Gln Gln Pro Trp Pro Ala Pro Pro Phe Asn Leu 370 375 380 Arg Gly Val
Glu Gly Val Glu Phe Ser Thr Pro Thr Asn Ser Phe Thr 385 390 395 400
Tyr Arg Gly Arg Gly Thr Val Asp Ser Leu Thr Glu Leu Pro Pro Glu 405
410 415 Asp Asn Ser Val Pro Pro Arg Glu Gly Tyr Ser His Arg Leu Cys
His 420 425 430 Ala Thr Phe Val Gln Arg Ser Gly Thr Pro Phe Leu Thr
Thr Gly Val 435 440 445 Val Phe Ser Trp Thr His Arg Ser Ala Thr Leu
Thr Asn Thr Ile Asp 450 455 460 Pro Glu Arg Ile Asn Gln Ile Pro Leu
Val Lys Gly Phe Arg Val Trp 465 470 475 480 Gly Gly Thr Ser Val Ile
Thr Gly Pro Gly Phe Thr Gly Gly Asp Ile 485 490 495 Leu Arg Arg Asn
Thr Phe Gly Asp Phe Val Ser Leu Gln Val Asn Ile 500 505 510 Asn Ser
Pro Ile Thr Gln Arg Tyr Arg Leu Arg Phe Arg Tyr Ala Ser 515 520 525
Ser Arg Asp Ala Arg Val Ile Val Leu Thr Gly Ala Ala Ser Thr Gly 530
535 540 Val Gly Gly Gln Val Ser Val Asn Met Pro Leu Gln Lys Thr Met
Glu 545 550 555 560 Ile Gly Glu Asn Leu Thr Ser Arg Thr Phe Arg Tyr
Thr Asp Phe Ser 565 570 575 Asn Pro Phe Ser Phe Arg Ala Asn Pro Asp
Ile Ile Gly Ile Ser Glu 580 585 590 Gln Pro Leu Phe Gly Ala Gly Ser
Ile Ser Ser Gly Glu Leu Tyr Ile 595 600 605 Asp Lys Ile Glu Ile Ile
Leu Ala Asp Ala Thr Phe Glu 610 615 620 271866DNAArtificial
SequenceSynthetic DNA sequence encoding Bacillus thuringiensis
Cry1Ca core toxin using codons optimized for maize and Table 1
sequences are maintainedCDS(1)..(1866) 27atg gat aac aat ccg aac
atc aat gag tgc atc ccg tac aac tgc ctg 48Met Asp Asn Asn Pro Asn
Ile Asn Glu Cys Ile Pro Tyr Asn Cys Leu 1 5 10 15 agc aac ccg gaa
gaa gtg ctg ttg gat gga gaa cgg ata tca act ggc 96Ser Asn Pro Glu
Glu Val Leu Leu Asp Gly Glu Arg Ile Ser Thr Gly 20 25 30 aat tca
tcc att gat att tct ctg tca ctt gtt cag ttt ctg gtg tct 144Asn Ser
Ser Ile Asp Ile Ser Leu Ser Leu Val Gln Phe Leu Val Ser 35 40 45
aac ttc gtc ccc ggc gga gga ttc ctg gtt gga tta ata gat ttt gta
192Asn Phe Val Pro Gly Gly Gly Phe Leu Val Gly Leu Ile Asp Phe Val
50 55 60 tgg gga ata gtt ggc cct tct caa tgg gac gca ttt cta gta
caa att 240Trp Gly Ile Val Gly Pro Ser Gln Trp Asp Ala Phe Leu Val
Gln Ile 65 70 75 80 gaa caa tta att aat gaa aga ata gct gaa ttt gct
agg aac gct gct 288Glu Gln Leu Ile Asn Glu Arg Ile Ala Glu Phe Ala
Arg Asn Ala Ala 85 90 95 att gct aat tta gaa gga tta gga aac aat
ttc aat ata tat gtg gaa 336Ile Ala Asn Leu Glu Gly Leu Gly Asn Asn
Phe Asn Ile Tyr Val Glu 100 105 110 gca ttt aag gaa tgg gaa gaa gat
cct aag aat cca gca acg agg acc 384Ala Phe Lys Glu Trp Glu Glu Asp
Pro Lys Asn Pro Ala Thr Arg Thr 115 120 125 aga gta att gat cgc ttt
cgt ata ctt gat ggg cta ctt gaa agg gac 432Arg Val Ile Asp Arg Phe
Arg Ile Leu Asp Gly Leu Leu Glu Arg Asp 130 135 140 att cct tcg ttt
cga att tct gga ttt gaa gta ccc ctt tta tcc gtt 480Ile Pro Ser Phe
Arg Ile Ser Gly Phe Glu Val Pro Leu Leu Ser Val 145 150 155 160 tat
gct caa gcg gcc aat ctg cat cta gct ata tta aga gat tct gta 528Tyr
Ala Gln Ala Ala Asn Leu His Leu Ala Ile Leu Arg Asp Ser Val 165 170
175 att ttt gga gaa aga tgg gga ttg aca acg ata aat gtc aat gaa aac
576Ile Phe Gly Glu Arg Trp Gly Leu Thr Thr Ile Asn Val Asn Glu Asn
180 185 190 tat aat aga cta att agg cat att gat gaa tat gct gat cac
tgt gca 624Tyr Asn Arg Leu Ile Arg His Ile Asp Glu Tyr Ala Asp His
Cys Ala 195 200 205 aat acg tat aat cgg gga tta aat aat tta ccg aaa
tct acg tat caa 672Asn Thr Tyr Asn Arg Gly Leu Asn Asn Leu Pro Lys
Ser Thr Tyr Gln 210 215 220 gat tgg ata aca tat aat cga ttg cgg aga
gac tta aca ttg act gta 720Asp Trp Ile Thr Tyr Asn Arg Leu Arg Arg
Asp Leu Thr Leu Thr Val 225 230 235 240 tta gat atc gcc gct ttc ttt
cca aac tat gac aat agg aga tat cca 768Leu Asp Ile Ala Ala Phe Phe
Pro Asn Tyr Asp Asn Arg Arg Tyr Pro 245 250 255 att cag cca gtt ggt
caa cta aca agg gaa gtt tat acg gac cca tta 816Ile Gln Pro Val Gly
Gln Leu Thr Arg Glu Val Tyr Thr Asp Pro Leu 260 265 270 att aat ttt
aat cca cag tta cag tct gta gct caa tta cct act ttt 864Ile
Asn Phe Asn Pro Gln Leu Gln Ser Val Ala Gln Leu Pro Thr Phe 275 280
285 aac gtt atg gag aac agc gca att aga aat cct cat tta ttt gat ata
912Asn Val Met Glu Asn Ser Ala Ile Arg Asn Pro His Leu Phe Asp Ile
290 295 300 ttg aat aat ctt aca atc ttt acg gat tgg ttt agt gtt gga
cgc aat 960Leu Asn Asn Leu Thr Ile Phe Thr Asp Trp Phe Ser Val Gly
Arg Asn 305 310 315 320 ttt tat tgg gga gga cat cga gta ata tct agc
ctt ata gga ggt ggg 1008Phe Tyr Trp Gly Gly His Arg Val Ile Ser Ser
Leu Ile Gly Gly Gly 325 330 335 aac atc aca tcg cct ata tat gga aga
gag gcg aac caa gag cct cca 1056Asn Ile Thr Ser Pro Ile Tyr Gly Arg
Glu Ala Asn Gln Glu Pro Pro 340 345 350 aga tcc ttt act ttt aat gga
ccc gtg ttt agg act tta tca aat cct 1104Arg Ser Phe Thr Phe Asn Gly
Pro Val Phe Arg Thr Leu Ser Asn Pro 355 360 365 act tta cga tta tta
cag caa cct tgg cca gcg cca cca ttt aat tta 1152Thr Leu Arg Leu Leu
Gln Gln Pro Trp Pro Ala Pro Pro Phe Asn Leu 370 375 380 cgt ggt gtt
gaa gga gta gaa ttt tct aca cct aca aat agc ttt acg 1200Arg Gly Val
Glu Gly Val Glu Phe Ser Thr Pro Thr Asn Ser Phe Thr 385 390 395 400
tat cga gga aga ggg acg gtt gat tct tta act gaa ttg ccg cct gag
1248Tyr Arg Gly Arg Gly Thr Val Asp Ser Leu Thr Glu Leu Pro Pro Glu
405 410 415 gat aat agt gtg cca cct cgc gaa gga tat agt cat cgt tta
tgt cat 1296Asp Asn Ser Val Pro Pro Arg Glu Gly Tyr Ser His Arg Leu
Cys His 420 425 430 gca act ttt gtt caa aga tcg gga aca cct ttt tta
aca act ggt gta 1344Ala Thr Phe Val Gln Arg Ser Gly Thr Pro Phe Leu
Thr Thr Gly Val 435 440 445 gta ttt tct tgg acg cat cgt agt gca act
ctt aca aat aca atc gac 1392Val Phe Ser Trp Thr His Arg Ser Ala Thr
Leu Thr Asn Thr Ile Asp 450 455 460 cca gag aga att aat caa ata cct
tta gtg aag gga ttt aga gtt tgg 1440Pro Glu Arg Ile Asn Gln Ile Pro
Leu Val Lys Gly Phe Arg Val Trp 465 470 475 480 ggg ggc acc tct gtc
att acc gga ccc gga ttt acc gga ggg gat atc 1488Gly Gly Thr Ser Val
Ile Thr Gly Pro Gly Phe Thr Gly Gly Asp Ile 485 490 495 ctt cga aga
aat acc ttt ggt gat ttt gta tct cta caa gtc aac att 1536Leu Arg Arg
Asn Thr Phe Gly Asp Phe Val Ser Leu Gln Val Asn Ile 500 505 510 aat
tca cca att acc caa aga tac cgt tta aga ttt cgt tac gct tcc 1584Asn
Ser Pro Ile Thr Gln Arg Tyr Arg Leu Arg Phe Arg Tyr Ala Ser 515 520
525 agt agg gat gca cga gtt ata gta tta acg gga gcg gca tcc acc gga
1632Ser Arg Asp Ala Arg Val Ile Val Leu Thr Gly Ala Ala Ser Thr Gly
530 535 540 gtg gga ggc caa gtt agt gta aat atg cct ctt cag aaa act
atg gaa 1680Val Gly Gly Gln Val Ser Val Asn Met Pro Leu Gln Lys Thr
Met Glu 545 550 555 560 ata ggg gag aac tta aca tcc aga aca ttt aga
tat acc gat ttt agt 1728Ile Gly Glu Asn Leu Thr Ser Arg Thr Phe Arg
Tyr Thr Asp Phe Ser 565 570 575 aat cct ttt tca ttt aga gct aat cca
gat ata att ggg ata agt gaa 1776Asn Pro Phe Ser Phe Arg Ala Asn Pro
Asp Ile Ile Gly Ile Ser Glu 580 585 590 caa cct cta ttt ggg gcg ggt
tct att agt agc ggt gaa ctt tat ata 1824Gln Pro Leu Phe Gly Ala Gly
Ser Ile Ser Ser Gly Glu Leu Tyr Ile 595 600 605 gat aaa att gaa att
att cta gca gat gca aca ttt gaa tga 1866Asp Lys Ile Glu Ile Ile Leu
Ala Asp Ala Thr Phe Glu 610 615 620 28621PRTArtificial
SequenceSynthetic Construct 28Met Asp Asn Asn Pro Asn Ile Asn Glu
Cys Ile Pro Tyr Asn Cys Leu 1 5 10 15 Ser Asn Pro Glu Glu Val Leu
Leu Asp Gly Glu Arg Ile Ser Thr Gly 20 25 30 Asn Ser Ser Ile Asp
Ile Ser Leu Ser Leu Val Gln Phe Leu Val Ser 35 40 45 Asn Phe Val
Pro Gly Gly Gly Phe Leu Val Gly Leu Ile Asp Phe Val 50 55 60 Trp
Gly Ile Val Gly Pro Ser Gln Trp Asp Ala Phe Leu Val Gln Ile 65 70
75 80 Glu Gln Leu Ile Asn Glu Arg Ile Ala Glu Phe Ala Arg Asn Ala
Ala 85 90 95 Ile Ala Asn Leu Glu Gly Leu Gly Asn Asn Phe Asn Ile
Tyr Val Glu 100 105 110 Ala Phe Lys Glu Trp Glu Glu Asp Pro Lys Asn
Pro Ala Thr Arg Thr 115 120 125 Arg Val Ile Asp Arg Phe Arg Ile Leu
Asp Gly Leu Leu Glu Arg Asp 130 135 140 Ile Pro Ser Phe Arg Ile Ser
Gly Phe Glu Val Pro Leu Leu Ser Val 145 150 155 160 Tyr Ala Gln Ala
Ala Asn Leu His Leu Ala Ile Leu Arg Asp Ser Val 165 170 175 Ile Phe
Gly Glu Arg Trp Gly Leu Thr Thr Ile Asn Val Asn Glu Asn 180 185 190
Tyr Asn Arg Leu Ile Arg His Ile Asp Glu Tyr Ala Asp His Cys Ala 195
200 205 Asn Thr Tyr Asn Arg Gly Leu Asn Asn Leu Pro Lys Ser Thr Tyr
Gln 210 215 220 Asp Trp Ile Thr Tyr Asn Arg Leu Arg Arg Asp Leu Thr
Leu Thr Val 225 230 235 240 Leu Asp Ile Ala Ala Phe Phe Pro Asn Tyr
Asp Asn Arg Arg Tyr Pro 245 250 255 Ile Gln Pro Val Gly Gln Leu Thr
Arg Glu Val Tyr Thr Asp Pro Leu 260 265 270 Ile Asn Phe Asn Pro Gln
Leu Gln Ser Val Ala Gln Leu Pro Thr Phe 275 280 285 Asn Val Met Glu
Asn Ser Ala Ile Arg Asn Pro His Leu Phe Asp Ile 290 295 300 Leu Asn
Asn Leu Thr Ile Phe Thr Asp Trp Phe Ser Val Gly Arg Asn 305 310 315
320 Phe Tyr Trp Gly Gly His Arg Val Ile Ser Ser Leu Ile Gly Gly Gly
325 330 335 Asn Ile Thr Ser Pro Ile Tyr Gly Arg Glu Ala Asn Gln Glu
Pro Pro 340 345 350 Arg Ser Phe Thr Phe Asn Gly Pro Val Phe Arg Thr
Leu Ser Asn Pro 355 360 365 Thr Leu Arg Leu Leu Gln Gln Pro Trp Pro
Ala Pro Pro Phe Asn Leu 370 375 380 Arg Gly Val Glu Gly Val Glu Phe
Ser Thr Pro Thr Asn Ser Phe Thr 385 390 395 400 Tyr Arg Gly Arg Gly
Thr Val Asp Ser Leu Thr Glu Leu Pro Pro Glu 405 410 415 Asp Asn Ser
Val Pro Pro Arg Glu Gly Tyr Ser His Arg Leu Cys His 420 425 430 Ala
Thr Phe Val Gln Arg Ser Gly Thr Pro Phe Leu Thr Thr Gly Val 435 440
445 Val Phe Ser Trp Thr His Arg Ser Ala Thr Leu Thr Asn Thr Ile Asp
450 455 460 Pro Glu Arg Ile Asn Gln Ile Pro Leu Val Lys Gly Phe Arg
Val Trp 465 470 475 480 Gly Gly Thr Ser Val Ile Thr Gly Pro Gly Phe
Thr Gly Gly Asp Ile 485 490 495 Leu Arg Arg Asn Thr Phe Gly Asp Phe
Val Ser Leu Gln Val Asn Ile 500 505 510 Asn Ser Pro Ile Thr Gln Arg
Tyr Arg Leu Arg Phe Arg Tyr Ala Ser 515 520 525 Ser Arg Asp Ala Arg
Val Ile Val Leu Thr Gly Ala Ala Ser Thr Gly 530 535 540 Val Gly Gly
Gln Val Ser Val Asn Met Pro Leu Gln Lys Thr Met Glu 545 550 555 560
Ile Gly Glu Asn Leu Thr Ser Arg Thr Phe Arg Tyr Thr Asp Phe Ser 565
570 575 Asn Pro Phe Ser Phe Arg Ala Asn Pro Asp Ile Ile Gly Ile Ser
Glu 580 585 590 Gln Pro Leu Phe Gly Ala Gly Ser Ile Ser Ser Gly Glu
Leu Tyr Ile 595 600 605 Asp Lys Ile Glu Ile Ile Leu Ala Asp Ala Thr
Phe Glu 610 615 620 291866DNAArtificial SequenceSynthetic DNA
sequence in accordance with the invention encoding Bacillus
thuringiensis Cry1Ca core toxin using codons optimized for maize
and with sequences identified in Table 2 removed and Table 1
sequences are maintainedCDS(1)..(1866) 29atg gat aac aat ccg aac
atc aat gag tgc atc ccg tac aac tgc ctg 48Met Asp Asn Asn Pro Asn
Ile Asn Glu Cys Ile Pro Tyr Asn Cys Leu 1 5 10 15 agc aac ccg gaa
gaa gtg ctg ttg gat gga gaa cgg ata tca act ggc 96Ser Asn Pro Glu
Glu Val Leu Leu Asp Gly Glu Arg Ile Ser Thr Gly 20 25 30 aat tca
tcc att gac att tct ctg tca ctt gtt cag ttt ctg gtg tct 144Asn Ser
Ser Ile Asp Ile Ser Leu Ser Leu Val Gln Phe Leu Val Ser 35 40 45
aac ttc gtc ccc ggc gga gga ttc ctg gtt gga tta ata gat ttc gta
192Asn Phe Val Pro Gly Gly Gly Phe Leu Val Gly Leu Ile Asp Phe Val
50 55 60 tgg gga ata gtt ggc cct tct caa tgg gac gca ttt cta gta
caa att 240Trp Gly Ile Val Gly Pro Ser Gln Trp Asp Ala Phe Leu Val
Gln Ile 65 70 75 80 gaa caa tta att aat gaa aga ata gct gaa ttt gct
agg aac gct gct 288Glu Gln Leu Ile Asn Glu Arg Ile Ala Glu Phe Ala
Arg Asn Ala Ala 85 90 95 att gct aat tta gaa gga tta gga aac aat
ttc aac atc tat gtg gaa 336Ile Ala Asn Leu Glu Gly Leu Gly Asn Asn
Phe Asn Ile Tyr Val Glu 100 105 110 gca ttt aag gaa tgg gaa gaa gat
cct aag aat cca gca acg agg acc 384Ala Phe Lys Glu Trp Glu Glu Asp
Pro Lys Asn Pro Ala Thr Arg Thr 115 120 125 aga gta att gat cgc ttt
cgt ata ctt gat ggg cta ctt gaa agg gac 432Arg Val Ile Asp Arg Phe
Arg Ile Leu Asp Gly Leu Leu Glu Arg Asp 130 135 140 att cct tcg ttt
cga att tct gga ttt gaa gta ccc ctt ctc tcc gtt 480Ile Pro Ser Phe
Arg Ile Ser Gly Phe Glu Val Pro Leu Leu Ser Val 145 150 155 160 tat
gct caa gcg gcc aat ctg cat cta gct atc tta aga gat tct gtc 528Tyr
Ala Gln Ala Ala Asn Leu His Leu Ala Ile Leu Arg Asp Ser Val 165 170
175 atc ttt gga gaa aga tgg gga ttg aca acg ata aat gtc aat gaa aac
576Ile Phe Gly Glu Arg Trp Gly Leu Thr Thr Ile Asn Val Asn Glu Asn
180 185 190 tat aat aga cta att agg cat att gat gaa tat gct gat cac
tgt gca 624Tyr Asn Arg Leu Ile Arg His Ile Asp Glu Tyr Ala Asp His
Cys Ala 195 200 205 aat acg tat aat cgg gga tta aat aat tta ccg aaa
tct acg tat caa 672Asn Thr Tyr Asn Arg Gly Leu Asn Asn Leu Pro Lys
Ser Thr Tyr Gln 210 215 220 gat tgg ata aca tat aat cga ttg cgg aga
gac tta aca ttg act gta 720Asp Trp Ile Thr Tyr Asn Arg Leu Arg Arg
Asp Leu Thr Leu Thr Val 225 230 235 240 tta gat atc gcc gct ttc ttt
cca aac tat gac aat agg aga tat cca 768Leu Asp Ile Ala Ala Phe Phe
Pro Asn Tyr Asp Asn Arg Arg Tyr Pro 245 250 255 att cag cca gtt ggt
caa cta aca agg gaa gtt tat acg gac cca tta 816Ile Gln Pro Val Gly
Gln Leu Thr Arg Glu Val Tyr Thr Asp Pro Leu 260 265 270 att aat ttt
aat cca cag tta cag tct gta gct caa tta cct act ttt 864Ile Asn Phe
Asn Pro Gln Leu Gln Ser Val Ala Gln Leu Pro Thr Phe 275 280 285 aac
gtt atg gag aac agc gca att aga aat cct cat ttg ttc gac ata 912Asn
Val Met Glu Asn Ser Ala Ile Arg Asn Pro His Leu Phe Asp Ile 290 295
300 ttg aat aat ctt aca atc ttt acg gat tgg ttt agt gtt gga cgc aac
960Leu Asn Asn Leu Thr Ile Phe Thr Asp Trp Phe Ser Val Gly Arg Asn
305 310 315 320 ttc tat tgg gga gga cat cga gta ata tct agc ctt ata
gga ggt ggg 1008Phe Tyr Trp Gly Gly His Arg Val Ile Ser Ser Leu Ile
Gly Gly Gly 325 330 335 aac atc aca tcg cct atc tat gga aga gag gcg
aac caa gag cct cca 1056Asn Ile Thr Ser Pro Ile Tyr Gly Arg Glu Ala
Asn Gln Glu Pro Pro 340 345 350 aga tcc ttt act ttt aat gga ccc gtg
ttt agg act tta tca aat cct 1104Arg Ser Phe Thr Phe Asn Gly Pro Val
Phe Arg Thr Leu Ser Asn Pro 355 360 365 act tta cga tta tta cag caa
cct tgg cca gcg cca cca ttt aat tta 1152Thr Leu Arg Leu Leu Gln Gln
Pro Trp Pro Ala Pro Pro Phe Asn Leu 370 375 380 cgt ggt gtt gaa gga
gta gaa ttt tct aca cct aca aat agc ttt acg 1200Arg Gly Val Glu Gly
Val Glu Phe Ser Thr Pro Thr Asn Ser Phe Thr 385 390 395 400 tat cga
gga aga ggg acg gtt gat tct tta act gaa ttg ccg cct gag 1248Tyr Arg
Gly Arg Gly Thr Val Asp Ser Leu Thr Glu Leu Pro Pro Glu 405 410 415
gat aat agt gtg cca cct cgc gaa gga tat agt cat cgt tta tgt cat
1296Asp Asn Ser Val Pro Pro Arg Glu Gly Tyr Ser His Arg Leu Cys His
420 425 430 gca acc ttt gtt caa aga tcg gga aca cct ttc tta aca act
ggt gta 1344Ala Thr Phe Val Gln Arg Ser Gly Thr Pro Phe Leu Thr Thr
Gly Val 435 440 445 gta ttc tct tgg acg cat cgt agt gca act ctt aca
aat aca atc gac 1392Val Phe Ser Trp Thr His Arg Ser Ala Thr Leu Thr
Asn Thr Ile Asp 450 455 460 cca gag aga att aat caa ata cct tta gtg
aag gga ttt aga gtt tgg 1440Pro Glu Arg Ile Asn Gln Ile Pro Leu Val
Lys Gly Phe Arg Val Trp 465 470 475 480 ggg ggc acc tct gtc att acc
gga ccc gga ttt acc gga ggg gat atc 1488Gly Gly Thr Ser Val Ile Thr
Gly Pro Gly Phe Thr Gly Gly Asp Ile 485 490 495 ctt cga aga aat acc
ttt ggt gat ttc gta tct cta caa gtc aac att 1536Leu Arg Arg Asn Thr
Phe Gly Asp Phe Val Ser Leu Gln Val Asn Ile 500 505 510 aat tca cca
att acc caa aga tac cgt tta aga ttt cgt tac gct tcc 1584Asn Ser Pro
Ile Thr Gln Arg Tyr Arg Leu Arg Phe Arg Tyr Ala Ser 515 520 525 agt
agg gat gca cga gtt ata gta tta acg gga gcg gca tcc acc gga 1632Ser
Arg Asp Ala Arg Val Ile Val Leu Thr Gly Ala Ala Ser Thr Gly 530 535
540 gtg gga ggc caa gtt agt gta aat atg cct ctt cag aaa act atg gaa
1680Val Gly Gly Gln Val Ser Val Asn Met Pro Leu Gln Lys Thr Met Glu
545 550 555 560 ata ggg gag aac tta aca tcc aga aca ttt aga tat acc
gat ttt agt 1728Ile Gly Glu Asn Leu Thr Ser Arg Thr Phe Arg Tyr Thr
Asp Phe Ser 565 570 575 aat cct ttt tca ttt aga gct aat cca gat ata
att ggg ata agt gaa 1776Asn Pro Phe Ser Phe Arg Ala Asn Pro Asp Ile
Ile Gly Ile Ser Glu 580 585 590 caa cct cta ttt ggg gcg ggt tct att
agt agc ggt gaa ctt tac ata 1824Gln Pro Leu Phe Gly Ala Gly Ser Ile
Ser Ser Gly Glu Leu Tyr Ile 595 600 605 gat aaa att gaa att att cta
gca gat gca aca ttt gaa tga 1866Asp Lys Ile Glu Ile Ile Leu Ala Asp
Ala Thr Phe Glu 610 615 620 30621PRTArtificial SequenceSynthetic
Construct 30Met Asp Asn Asn Pro Asn Ile Asn Glu Cys Ile Pro Tyr Asn
Cys Leu 1 5 10 15 Ser Asn Pro Glu Glu Val Leu Leu Asp Gly Glu Arg
Ile Ser Thr Gly 20 25 30 Asn Ser Ser Ile Asp Ile Ser Leu Ser Leu
Val Gln Phe Leu Val Ser 35
40 45 Asn Phe Val Pro Gly Gly Gly Phe Leu Val Gly Leu Ile Asp Phe
Val 50 55 60 Trp Gly Ile Val Gly Pro Ser Gln Trp Asp Ala Phe Leu
Val Gln Ile 65 70 75 80 Glu Gln Leu Ile Asn Glu Arg Ile Ala Glu Phe
Ala Arg Asn Ala Ala 85 90 95 Ile Ala Asn Leu Glu Gly Leu Gly Asn
Asn Phe Asn Ile Tyr Val Glu 100 105 110 Ala Phe Lys Glu Trp Glu Glu
Asp Pro Lys Asn Pro Ala Thr Arg Thr 115 120 125 Arg Val Ile Asp Arg
Phe Arg Ile Leu Asp Gly Leu Leu Glu Arg Asp 130 135 140 Ile Pro Ser
Phe Arg Ile Ser Gly Phe Glu Val Pro Leu Leu Ser Val 145 150 155 160
Tyr Ala Gln Ala Ala Asn Leu His Leu Ala Ile Leu Arg Asp Ser Val 165
170 175 Ile Phe Gly Glu Arg Trp Gly Leu Thr Thr Ile Asn Val Asn Glu
Asn 180 185 190 Tyr Asn Arg Leu Ile Arg His Ile Asp Glu Tyr Ala Asp
His Cys Ala 195 200 205 Asn Thr Tyr Asn Arg Gly Leu Asn Asn Leu Pro
Lys Ser Thr Tyr Gln 210 215 220 Asp Trp Ile Thr Tyr Asn Arg Leu Arg
Arg Asp Leu Thr Leu Thr Val 225 230 235 240 Leu Asp Ile Ala Ala Phe
Phe Pro Asn Tyr Asp Asn Arg Arg Tyr Pro 245 250 255 Ile Gln Pro Val
Gly Gln Leu Thr Arg Glu Val Tyr Thr Asp Pro Leu 260 265 270 Ile Asn
Phe Asn Pro Gln Leu Gln Ser Val Ala Gln Leu Pro Thr Phe 275 280 285
Asn Val Met Glu Asn Ser Ala Ile Arg Asn Pro His Leu Phe Asp Ile 290
295 300 Leu Asn Asn Leu Thr Ile Phe Thr Asp Trp Phe Ser Val Gly Arg
Asn 305 310 315 320 Phe Tyr Trp Gly Gly His Arg Val Ile Ser Ser Leu
Ile Gly Gly Gly 325 330 335 Asn Ile Thr Ser Pro Ile Tyr Gly Arg Glu
Ala Asn Gln Glu Pro Pro 340 345 350 Arg Ser Phe Thr Phe Asn Gly Pro
Val Phe Arg Thr Leu Ser Asn Pro 355 360 365 Thr Leu Arg Leu Leu Gln
Gln Pro Trp Pro Ala Pro Pro Phe Asn Leu 370 375 380 Arg Gly Val Glu
Gly Val Glu Phe Ser Thr Pro Thr Asn Ser Phe Thr 385 390 395 400 Tyr
Arg Gly Arg Gly Thr Val Asp Ser Leu Thr Glu Leu Pro Pro Glu 405 410
415 Asp Asn Ser Val Pro Pro Arg Glu Gly Tyr Ser His Arg Leu Cys His
420 425 430 Ala Thr Phe Val Gln Arg Ser Gly Thr Pro Phe Leu Thr Thr
Gly Val 435 440 445 Val Phe Ser Trp Thr His Arg Ser Ala Thr Leu Thr
Asn Thr Ile Asp 450 455 460 Pro Glu Arg Ile Asn Gln Ile Pro Leu Val
Lys Gly Phe Arg Val Trp 465 470 475 480 Gly Gly Thr Ser Val Ile Thr
Gly Pro Gly Phe Thr Gly Gly Asp Ile 485 490 495 Leu Arg Arg Asn Thr
Phe Gly Asp Phe Val Ser Leu Gln Val Asn Ile 500 505 510 Asn Ser Pro
Ile Thr Gln Arg Tyr Arg Leu Arg Phe Arg Tyr Ala Ser 515 520 525 Ser
Arg Asp Ala Arg Val Ile Val Leu Thr Gly Ala Ala Ser Thr Gly 530 535
540 Val Gly Gly Gln Val Ser Val Asn Met Pro Leu Gln Lys Thr Met Glu
545 550 555 560 Ile Gly Glu Asn Leu Thr Ser Arg Thr Phe Arg Tyr Thr
Asp Phe Ser 565 570 575 Asn Pro Phe Ser Phe Arg Ala Asn Pro Asp Ile
Ile Gly Ile Ser Glu 580 585 590 Gln Pro Leu Phe Gly Ala Gly Ser Ile
Ser Ser Gly Glu Leu Tyr Ile 595 600 605 Asp Lys Ile Glu Ile Ile Leu
Ala Asp Ala Thr Phe Glu 610 615 620 311428DNABacillus
thuringiensisCDS(1)..(1428)Native DNA sequence encoding Bacillus
thuringiensis Cry6Aa toxin 31atg att att gat agt aaa acg act tta
cct aga cat tca ctt att cat 48Met Ile Ile Asp Ser Lys Thr Thr Leu
Pro Arg His Ser Leu Ile His 1 5 10 15 aca att aaa tta aat tct aat
aag aaa tat ggt cct ggt gat atg act 96Thr Ile Lys Leu Asn Ser Asn
Lys Lys Tyr Gly Pro Gly Asp Met Thr 20 25 30 aat gga aat caa ttt
att att tca aaa caa gaa tgg gct acg att gga 144Asn Gly Asn Gln Phe
Ile Ile Ser Lys Gln Glu Trp Ala Thr Ile Gly 35 40 45 gca tat att
cag act gga tta ggt tta cca gta aat gaa caa caa tta 192Ala Tyr Ile
Gln Thr Gly Leu Gly Leu Pro Val Asn Glu Gln Gln Leu 50 55 60 aga
aca cat gtt aat tta agt cag gat ata tca ata cct agt gat ttt 240Arg
Thr His Val Asn Leu Ser Gln Asp Ile Ser Ile Pro Ser Asp Phe 65 70
75 80 tct caa tta tat gat gtt tat tgt tct gat aaa act tca gca gaa
tgg 288Ser Gln Leu Tyr Asp Val Tyr Cys Ser Asp Lys Thr Ser Ala Glu
Trp 85 90 95 tgg aat aaa aat tta tat cct tta att att aaa tct gct
aat gat att 336Trp Asn Lys Asn Leu Tyr Pro Leu Ile Ile Lys Ser Ala
Asn Asp Ile 100 105 110 gct tca tat ggt ttt aaa gtt gct ggt gat cct
tct att aag aaa gat 384Ala Ser Tyr Gly Phe Lys Val Ala Gly Asp Pro
Ser Ile Lys Lys Asp 115 120 125 gga tat ttt aaa aaa ttg caa gat gaa
tta gat aat att gtt gat aat 432Gly Tyr Phe Lys Lys Leu Gln Asp Glu
Leu Asp Asn Ile Val Asp Asn 130 135 140 aat tcc gat gat gat gca ata
gct aaa gct att aaa gat ttt aaa gcg 480Asn Ser Asp Asp Asp Ala Ile
Ala Lys Ala Ile Lys Asp Phe Lys Ala 145 150 155 160 cga tgt ggt att
tta att aaa gaa gct aaa caa tat gaa gaa gct gca 528Arg Cys Gly Ile
Leu Ile Lys Glu Ala Lys Gln Tyr Glu Glu Ala Ala 165 170 175 aaa aat
att gta aca tct tta gat caa ttt tta cat ggt gat cag aaa 576Lys Asn
Ile Val Thr Ser Leu Asp Gln Phe Leu His Gly Asp Gln Lys 180 185 190
aaa tta gaa ggt gtt atc aat att caa aaa cgt tta aaa gaa gtt caa
624Lys Leu Glu Gly Val Ile Asn Ile Gln Lys Arg Leu Lys Glu Val Gln
195 200 205 aca gct ctt aat caa gcc cat ggg gaa agt agt cca gct cat
aaa gag 672Thr Ala Leu Asn Gln Ala His Gly Glu Ser Ser Pro Ala His
Lys Glu 210 215 220 tta tta gaa aaa gta aaa aat tta aaa aca aca tta
gaa agg act att 720Leu Leu Glu Lys Val Lys Asn Leu Lys Thr Thr Leu
Glu Arg Thr Ile 225 230 235 240 aaa gct gaa caa gat tta gag aaa aaa
gta gaa tat agt ttt cta tta 768Lys Ala Glu Gln Asp Leu Glu Lys Lys
Val Glu Tyr Ser Phe Leu Leu 245 250 255 gga cca ttg tta gga ttt gtt
gtt tat gaa att ctt gaa aat act gct 816Gly Pro Leu Leu Gly Phe Val
Val Tyr Glu Ile Leu Glu Asn Thr Ala 260 265 270 gtt cag cat ata aaa
aat caa att gat gag ata aag aaa caa tta gat 864Val Gln His Ile Lys
Asn Gln Ile Asp Glu Ile Lys Lys Gln Leu Asp 275 280 285 tct gct cag
cat gat ttg gat aga gat gtt aaa att ata gga atg tta 912Ser Ala Gln
His Asp Leu Asp Arg Asp Val Lys Ile Ile Gly Met Leu 290 295 300 aat
agt att aat aca gat att gat aat tta tat agt caa gga caa gaa 960Asn
Ser Ile Asn Thr Asp Ile Asp Asn Leu Tyr Ser Gln Gly Gln Glu 305 310
315 320 gca att aaa gtt ttc caa aag tta caa ggt att tgg gct act att
gga 1008Ala Ile Lys Val Phe Gln Lys Leu Gln Gly Ile Trp Ala Thr Ile
Gly 325 330 335 gct caa ata gaa aat ctt aga aca acg tcg tta caa gaa
gtt caa gat 1056Ala Gln Ile Glu Asn Leu Arg Thr Thr Ser Leu Gln Glu
Val Gln Asp 340 345 350 tct gat gat gct gat gag ata caa att gaa ctt
gag gac gct tct gat 1104Ser Asp Asp Ala Asp Glu Ile Gln Ile Glu Leu
Glu Asp Ala Ser Asp 355 360 365 gct tgg tta gtt gtg gct caa gaa gct
cgt gat ttt aca cta aat gct 1152Ala Trp Leu Val Val Ala Gln Glu Ala
Arg Asp Phe Thr Leu Asn Ala 370 375 380 tat tca act aat agt aga caa
aat tta ccg att aat gtt ata tca gat 1200Tyr Ser Thr Asn Ser Arg Gln
Asn Leu Pro Ile Asn Val Ile Ser Asp 385 390 395 400 tca tgt aat tgt
tca aca aca aat atg aca tca aat caa tac agt aat 1248Ser Cys Asn Cys
Ser Thr Thr Asn Met Thr Ser Asn Gln Tyr Ser Asn 405 410 415 cca aca
aca aat atg aca tca aat caa tat atg att tca cat gaa tat 1296Pro Thr
Thr Asn Met Thr Ser Asn Gln Tyr Met Ile Ser His Glu Tyr 420 425 430
aca agt tta cca aat aat ttt atg tta tca aga aat agt aat tta gaa
1344Thr Ser Leu Pro Asn Asn Phe Met Leu Ser Arg Asn Ser Asn Leu Glu
435 440 445 tat aaa tgt cct gaa aat aat ttt atg ata tat tgg tat aat
aat tcg 1392Tyr Lys Cys Pro Glu Asn Asn Phe Met Ile Tyr Trp Tyr Asn
Asn Ser 450 455 460 gat tgg tat aat aat tcg gat tgg tat aat aat tga
1428Asp Trp Tyr Asn Asn Ser Asp Trp Tyr Asn Asn 465 470 475
32475PRTBacillus thuringiensis 32Met Ile Ile Asp Ser Lys Thr Thr
Leu Pro Arg His Ser Leu Ile His 1 5 10 15 Thr Ile Lys Leu Asn Ser
Asn Lys Lys Tyr Gly Pro Gly Asp Met Thr 20 25 30 Asn Gly Asn Gln
Phe Ile Ile Ser Lys Gln Glu Trp Ala Thr Ile Gly 35 40 45 Ala Tyr
Ile Gln Thr Gly Leu Gly Leu Pro Val Asn Glu Gln Gln Leu 50 55 60
Arg Thr His Val Asn Leu Ser Gln Asp Ile Ser Ile Pro Ser Asp Phe 65
70 75 80 Ser Gln Leu Tyr Asp Val Tyr Cys Ser Asp Lys Thr Ser Ala
Glu Trp 85 90 95 Trp Asn Lys Asn Leu Tyr Pro Leu Ile Ile Lys Ser
Ala Asn Asp Ile 100 105 110 Ala Ser Tyr Gly Phe Lys Val Ala Gly Asp
Pro Ser Ile Lys Lys Asp 115 120 125 Gly Tyr Phe Lys Lys Leu Gln Asp
Glu Leu Asp Asn Ile Val Asp Asn 130 135 140 Asn Ser Asp Asp Asp Ala
Ile Ala Lys Ala Ile Lys Asp Phe Lys Ala 145 150 155 160 Arg Cys Gly
Ile Leu Ile Lys Glu Ala Lys Gln Tyr Glu Glu Ala Ala 165 170 175 Lys
Asn Ile Val Thr Ser Leu Asp Gln Phe Leu His Gly Asp Gln Lys 180 185
190 Lys Leu Glu Gly Val Ile Asn Ile Gln Lys Arg Leu Lys Glu Val Gln
195 200 205 Thr Ala Leu Asn Gln Ala His Gly Glu Ser Ser Pro Ala His
Lys Glu 210 215 220 Leu Leu Glu Lys Val Lys Asn Leu Lys Thr Thr Leu
Glu Arg Thr Ile 225 230 235 240 Lys Ala Glu Gln Asp Leu Glu Lys Lys
Val Glu Tyr Ser Phe Leu Leu 245 250 255 Gly Pro Leu Leu Gly Phe Val
Val Tyr Glu Ile Leu Glu Asn Thr Ala 260 265 270 Val Gln His Ile Lys
Asn Gln Ile Asp Glu Ile Lys Lys Gln Leu Asp 275 280 285 Ser Ala Gln
His Asp Leu Asp Arg Asp Val Lys Ile Ile Gly Met Leu 290 295 300 Asn
Ser Ile Asn Thr Asp Ile Asp Asn Leu Tyr Ser Gln Gly Gln Glu 305 310
315 320 Ala Ile Lys Val Phe Gln Lys Leu Gln Gly Ile Trp Ala Thr Ile
Gly 325 330 335 Ala Gln Ile Glu Asn Leu Arg Thr Thr Ser Leu Gln Glu
Val Gln Asp 340 345 350 Ser Asp Asp Ala Asp Glu Ile Gln Ile Glu Leu
Glu Asp Ala Ser Asp 355 360 365 Ala Trp Leu Val Val Ala Gln Glu Ala
Arg Asp Phe Thr Leu Asn Ala 370 375 380 Tyr Ser Thr Asn Ser Arg Gln
Asn Leu Pro Ile Asn Val Ile Ser Asp 385 390 395 400 Ser Cys Asn Cys
Ser Thr Thr Asn Met Thr Ser Asn Gln Tyr Ser Asn 405 410 415 Pro Thr
Thr Asn Met Thr Ser Asn Gln Tyr Met Ile Ser His Glu Tyr 420 425 430
Thr Ser Leu Pro Asn Asn Phe Met Leu Ser Arg Asn Ser Asn Leu Glu 435
440 445 Tyr Lys Cys Pro Glu Asn Asn Phe Met Ile Tyr Trp Tyr Asn Asn
Ser 450 455 460 Asp Trp Tyr Asn Asn Ser Asp Trp Tyr Asn Asn 465 470
475 331428DNAArtificial SequenceSynthetic DNA sequence encoding
Bacillus thuringiensis Cry6Aa toxin using codons optimized for
maize and Table 1 sequences are maintainedCDS(1)..(1428) 33atg atc
atc gac tcc aag acg acc ctg cca cgg cac tcc ctt atc cac 48Met Ile
Ile Asp Ser Lys Thr Thr Leu Pro Arg His Ser Leu Ile His 1 5 10 15
aca att aaa tta aat agc aat aag aag tac ggt ccc ggt gat atg act
96Thr Ile Lys Leu Asn Ser Asn Lys Lys Tyr Gly Pro Gly Asp Met Thr
20 25 30 aac gga aat caa ttc att att tca aag caa gag tgg gct acc
atc gga 144Asn Gly Asn Gln Phe Ile Ile Ser Lys Gln Glu Trp Ala Thr
Ile Gly 35 40 45 gcg tac atc cag act ggg ctg ggc cta cca gta aat
gaa caa caa tta 192Ala Tyr Ile Gln Thr Gly Leu Gly Leu Pro Val Asn
Glu Gln Gln Leu 50 55 60 agg acc cat gtc aac ctc agc caa gat atc
agc atc cct agc gac ttt 240Arg Thr His Val Asn Leu Ser Gln Asp Ile
Ser Ile Pro Ser Asp Phe 65 70 75 80 tct cag ctc tac gac gtc tat tgc
agc gat aaa act tcc gca gaa tgg 288Ser Gln Leu Tyr Asp Val Tyr Cys
Ser Asp Lys Thr Ser Ala Glu Trp 85 90 95 tgg aat aaa aac ctg tac
ccc ctc atc att aaa tct gcc aac gat att 336Trp Asn Lys Asn Leu Tyr
Pro Leu Ile Ile Lys Ser Ala Asn Asp Ile 100 105 110 gcc agc tac ggc
ttc aag gtc gcg ggt gat cct tct att aag aag gac 384Ala Ser Tyr Gly
Phe Lys Val Ala Gly Asp Pro Ser Ile Lys Lys Asp 115 120 125 ggc tac
ttc aag aag ctg caa gat gag ctg gac aac att gtt gac aat 432Gly Tyr
Phe Lys Lys Leu Gln Asp Glu Leu Asp Asn Ile Val Asp Asn 130 135 140
aat tcc gat gat gat gca ata gcg aaa gcc att aaa gac ttc aag gcg
480Asn Ser Asp Asp Asp Ala Ile Ala Lys Ala Ile Lys Asp Phe Lys Ala
145 150 155 160 cga tgc ggc atc cta att aaa gaa gca aag cag tat gaa
gag gca gcg 528Arg Cys Gly Ile Leu Ile Lys Glu Ala Lys Gln Tyr Glu
Glu Ala Ala 165 170 175 aaa aat atc gta aca tcc ctc gac caa ttt ctg
cat ggc gat cag aag 576Lys Asn Ile Val Thr Ser Leu Asp Gln Phe Leu
His Gly Asp Gln Lys 180 185 190 aaa ttg gag ggt gtg atc aac atc caa
aaa cgt ctg aag gag gtg cag 624Lys Leu Glu Gly Val Ile Asn Ile Gln
Lys Arg Leu Lys Glu Val Gln 195 200 205 acg gct ctt aat caa gcc cac
ggg gaa agt tca cca gct cat aaa gag 672Thr Ala Leu Asn Gln Ala His
Gly Glu Ser Ser Pro Ala His Lys Glu 210 215 220 ctg tta gag aaa gtc
aag aat ctc aag acc aca ctt gag agg acc att 720Leu Leu Glu Lys Val
Lys Asn Leu Lys Thr Thr Leu Glu Arg Thr Ile 225 230 235 240 aaa gct
gag caa gac ctg gag aag aaa gtg gag tac agt ttc ctt ctc 768Lys Ala
Glu Gln Asp Leu Glu Lys Lys Val Glu Tyr Ser Phe Leu Leu
245 250 255 ggc ccc ttg ctg ggc ttc gtc gtt tat gaa atc ctt gaa aat
act gcc 816Gly Pro Leu Leu Gly Phe Val Val Tyr Glu Ile Leu Glu Asn
Thr Ala 260 265 270 gtc cag cat ata aaa aac caa att gac gag ata aag
aag caa ctg gac 864Val Gln His Ile Lys Asn Gln Ile Asp Glu Ile Lys
Lys Gln Leu Asp 275 280 285 tct gcc cag cac gac ttg gac aga gac gtt
aag atc ata ggg atg ctg 912Ser Ala Gln His Asp Leu Asp Arg Asp Val
Lys Ile Ile Gly Met Leu 290 295 300 aac agt att aat aca gac att gat
aac ttg tat agc caa gga caa gag 960Asn Ser Ile Asn Thr Asp Ile Asp
Asn Leu Tyr Ser Gln Gly Gln Glu 305 310 315 320 gca att aaa gtg ttc
caa aag ctc caa ggc atc tgg gca act atc gga 1008Ala Ile Lys Val Phe
Gln Lys Leu Gln Gly Ile Trp Ala Thr Ile Gly 325 330 335 gcg cag ata
gag aac ctt agg aca acg tcg ctc caa gaa gtg caa gac 1056Ala Gln Ile
Glu Asn Leu Arg Thr Thr Ser Leu Gln Glu Val Gln Asp 340 345 350 tct
gac gac gcc gat gag atc caa att gaa ctt gag gac gcg tct gat 1104Ser
Asp Asp Ala Asp Glu Ile Gln Ile Glu Leu Glu Asp Ala Ser Asp 355 360
365 gct tgg tta gtg gtg gcc caa gaa gct cgc gac ttc aca cta aat gcc
1152Ala Trp Leu Val Val Ala Gln Glu Ala Arg Asp Phe Thr Leu Asn Ala
370 375 380 tac tca act aac tcg cgt cag aat cta ccg att aat gtt ata
tcc gat 1200Tyr Ser Thr Asn Ser Arg Gln Asn Leu Pro Ile Asn Val Ile
Ser Asp 385 390 395 400 tcc tgc aac tgt tcc aca acg aac atg acc tca
aat caa tac agt aat 1248Ser Cys Asn Cys Ser Thr Thr Asn Met Thr Ser
Asn Gln Tyr Ser Asn 405 410 415 cca acc aca aat atg acc tca aat caa
tat atg atc tca cac gag tat 1296Pro Thr Thr Asn Met Thr Ser Asn Gln
Tyr Met Ile Ser His Glu Tyr 420 425 430 acc tcg ttg ccg aat aat ttc
atg ctc tca aga aat agc aat ctg gaa 1344Thr Ser Leu Pro Asn Asn Phe
Met Leu Ser Arg Asn Ser Asn Leu Glu 435 440 445 tat aag tgt cct gaa
aat aat ttc atg ata tac tgg tac aat aat tcg 1392Tyr Lys Cys Pro Glu
Asn Asn Phe Met Ile Tyr Trp Tyr Asn Asn Ser 450 455 460 gac tgg tac
aat aat tcg gat tgg tac aat aat tga 1428Asp Trp Tyr Asn Asn Ser Asp
Trp Tyr Asn Asn 465 470 475 34475PRTArtificial SequenceSynthetic
Construct 34Met Ile Ile Asp Ser Lys Thr Thr Leu Pro Arg His Ser Leu
Ile His 1 5 10 15 Thr Ile Lys Leu Asn Ser Asn Lys Lys Tyr Gly Pro
Gly Asp Met Thr 20 25 30 Asn Gly Asn Gln Phe Ile Ile Ser Lys Gln
Glu Trp Ala Thr Ile Gly 35 40 45 Ala Tyr Ile Gln Thr Gly Leu Gly
Leu Pro Val Asn Glu Gln Gln Leu 50 55 60 Arg Thr His Val Asn Leu
Ser Gln Asp Ile Ser Ile Pro Ser Asp Phe 65 70 75 80 Ser Gln Leu Tyr
Asp Val Tyr Cys Ser Asp Lys Thr Ser Ala Glu Trp 85 90 95 Trp Asn
Lys Asn Leu Tyr Pro Leu Ile Ile Lys Ser Ala Asn Asp Ile 100 105 110
Ala Ser Tyr Gly Phe Lys Val Ala Gly Asp Pro Ser Ile Lys Lys Asp 115
120 125 Gly Tyr Phe Lys Lys Leu Gln Asp Glu Leu Asp Asn Ile Val Asp
Asn 130 135 140 Asn Ser Asp Asp Asp Ala Ile Ala Lys Ala Ile Lys Asp
Phe Lys Ala 145 150 155 160 Arg Cys Gly Ile Leu Ile Lys Glu Ala Lys
Gln Tyr Glu Glu Ala Ala 165 170 175 Lys Asn Ile Val Thr Ser Leu Asp
Gln Phe Leu His Gly Asp Gln Lys 180 185 190 Lys Leu Glu Gly Val Ile
Asn Ile Gln Lys Arg Leu Lys Glu Val Gln 195 200 205 Thr Ala Leu Asn
Gln Ala His Gly Glu Ser Ser Pro Ala His Lys Glu 210 215 220 Leu Leu
Glu Lys Val Lys Asn Leu Lys Thr Thr Leu Glu Arg Thr Ile 225 230 235
240 Lys Ala Glu Gln Asp Leu Glu Lys Lys Val Glu Tyr Ser Phe Leu Leu
245 250 255 Gly Pro Leu Leu Gly Phe Val Val Tyr Glu Ile Leu Glu Asn
Thr Ala 260 265 270 Val Gln His Ile Lys Asn Gln Ile Asp Glu Ile Lys
Lys Gln Leu Asp 275 280 285 Ser Ala Gln His Asp Leu Asp Arg Asp Val
Lys Ile Ile Gly Met Leu 290 295 300 Asn Ser Ile Asn Thr Asp Ile Asp
Asn Leu Tyr Ser Gln Gly Gln Glu 305 310 315 320 Ala Ile Lys Val Phe
Gln Lys Leu Gln Gly Ile Trp Ala Thr Ile Gly 325 330 335 Ala Gln Ile
Glu Asn Leu Arg Thr Thr Ser Leu Gln Glu Val Gln Asp 340 345 350 Ser
Asp Asp Ala Asp Glu Ile Gln Ile Glu Leu Glu Asp Ala Ser Asp 355 360
365 Ala Trp Leu Val Val Ala Gln Glu Ala Arg Asp Phe Thr Leu Asn Ala
370 375 380 Tyr Ser Thr Asn Ser Arg Gln Asn Leu Pro Ile Asn Val Ile
Ser Asp 385 390 395 400 Ser Cys Asn Cys Ser Thr Thr Asn Met Thr Ser
Asn Gln Tyr Ser Asn 405 410 415 Pro Thr Thr Asn Met Thr Ser Asn Gln
Tyr Met Ile Ser His Glu Tyr 420 425 430 Thr Ser Leu Pro Asn Asn Phe
Met Leu Ser Arg Asn Ser Asn Leu Glu 435 440 445 Tyr Lys Cys Pro Glu
Asn Asn Phe Met Ile Tyr Trp Tyr Asn Asn Ser 450 455 460 Asp Trp Tyr
Asn Asn Ser Asp Trp Tyr Asn Asn 465 470 475 351428DNAArtificial
SequenceSynthetic DNA sequence in accordance with the invention
encoding Bacillus thuringiensis Cry6Aa toxin using codons optimized
for maize and with sequences identified in Table 2 removed and
Table 1 sequences are maintainedCDS(1)..(1428) 35atg atc atc gac
tcc aag acg acc ctg cca cgg cac tcc ctt atc cac 48Met Ile Ile Asp
Ser Lys Thr Thr Leu Pro Arg His Ser Leu Ile His 1 5 10 15 aca att
aaa tta aat agc aat aag aag tac ggt ccc ggt gat atg act 96Thr Ile
Lys Leu Asn Ser Asn Lys Lys Tyr Gly Pro Gly Asp Met Thr 20 25 30
aac gga aat caa ttc att atc tca aag caa gag tgg gct acc atc gga
144Asn Gly Asn Gln Phe Ile Ile Ser Lys Gln Glu Trp Ala Thr Ile Gly
35 40 45 gcg tac atc cag act ggg ctg ggc cta cca gta aat gaa caa
caa tta 192Ala Tyr Ile Gln Thr Gly Leu Gly Leu Pro Val Asn Glu Gln
Gln Leu 50 55 60 agg acc cat gtc aac ctc agc caa gat atc agc atc
cct agc gac ttt 240Arg Thr His Val Asn Leu Ser Gln Asp Ile Ser Ile
Pro Ser Asp Phe 65 70 75 80 tct cag ctc tac gac gtc tat tgc agc gat
aaa act tcc gca gaa tgg 288Ser Gln Leu Tyr Asp Val Tyr Cys Ser Asp
Lys Thr Ser Ala Glu Trp 85 90 95 tgg aat aaa aac ctg tac ccc ctc
atc att aaa tct gcc aac gat att 336Trp Asn Lys Asn Leu Tyr Pro Leu
Ile Ile Lys Ser Ala Asn Asp Ile 100 105 110 gcc agc tac ggc ttc aag
gtc gcg ggt gat cct tct att aag aag gac 384Ala Ser Tyr Gly Phe Lys
Val Ala Gly Asp Pro Ser Ile Lys Lys Asp 115 120 125 ggc tac ttc aag
aag ctg caa gat gag ctg gac aac att gtt gac aat 432Gly Tyr Phe Lys
Lys Leu Gln Asp Glu Leu Asp Asn Ile Val Asp Asn 130 135 140 aat tcc
gat gat gat gca ata gcg aaa gcc att aaa gac ttc aag gcg 480Asn Ser
Asp Asp Asp Ala Ile Ala Lys Ala Ile Lys Asp Phe Lys Ala 145 150 155
160 cga tgc ggc atc cta att aaa gaa gca aag cag tat gaa gag gca gcg
528Arg Cys Gly Ile Leu Ile Lys Glu Ala Lys Gln Tyr Glu Glu Ala Ala
165 170 175 aaa aat atc gta aca tcc ctc gac caa ttt ctg cat ggc gat
cag aag 576Lys Asn Ile Val Thr Ser Leu Asp Gln Phe Leu His Gly Asp
Gln Lys 180 185 190 aaa ttg gag ggt gtg atc aac atc caa aaa cgt ctg
aag gag gtg cag 624Lys Leu Glu Gly Val Ile Asn Ile Gln Lys Arg Leu
Lys Glu Val Gln 195 200 205 acg gct ctt aat caa gcc cac ggg gaa agt
tca cca gct cat aaa gag 672Thr Ala Leu Asn Gln Ala His Gly Glu Ser
Ser Pro Ala His Lys Glu 210 215 220 ctg tta gag aaa gtc aag aat ctc
aag acc aca ctt gag agg acc att 720Leu Leu Glu Lys Val Lys Asn Leu
Lys Thr Thr Leu Glu Arg Thr Ile 225 230 235 240 aaa gct gag caa gac
ctg gag aag aaa gtg gag tac agt ttc ctt ctc 768Lys Ala Glu Gln Asp
Leu Glu Lys Lys Val Glu Tyr Ser Phe Leu Leu 245 250 255 ggc ccc ttg
ctg ggc ttc gtc gtt tat gaa atc ctt gaa aat act gcc 816Gly Pro Leu
Leu Gly Phe Val Val Tyr Glu Ile Leu Glu Asn Thr Ala 260 265 270 gtc
cag cat ata aaa aac caa att gac gag ata aag aag caa ctg gac 864Val
Gln His Ile Lys Asn Gln Ile Asp Glu Ile Lys Lys Gln Leu Asp 275 280
285 tct gcc cag cac gac ttg gac aga gac gtt aag atc ata ggg atg ctg
912Ser Ala Gln His Asp Leu Asp Arg Asp Val Lys Ile Ile Gly Met Leu
290 295 300 aac agt att aat aca gac att gat aac ttg tat agc caa gga
caa gag 960Asn Ser Ile Asn Thr Asp Ile Asp Asn Leu Tyr Ser Gln Gly
Gln Glu 305 310 315 320 gca att aaa gtg ttc caa aag ctc caa ggc atc
tgg gca act atc gga 1008Ala Ile Lys Val Phe Gln Lys Leu Gln Gly Ile
Trp Ala Thr Ile Gly 325 330 335 gcg cag ata gag aac ctt agg aca acg
tcg ctc caa gaa gtg caa gac 1056Ala Gln Ile Glu Asn Leu Arg Thr Thr
Ser Leu Gln Glu Val Gln Asp 340 345 350 tct gac gac gcc gat gag atc
caa att gaa ctt gag gac gcg tct gat 1104Ser Asp Asp Ala Asp Glu Ile
Gln Ile Glu Leu Glu Asp Ala Ser Asp 355 360 365 gct tgg tta gtg gtg
gcc caa gaa gct cgc gac ttc aca cta aat gcc 1152Ala Trp Leu Val Val
Ala Gln Glu Ala Arg Asp Phe Thr Leu Asn Ala 370 375 380 tac tca act
aac tcg cgt cag aat cta ccg att aat gtt atc tcc gat 1200Tyr Ser Thr
Asn Ser Arg Gln Asn Leu Pro Ile Asn Val Ile Ser Asp 385 390 395 400
tcc tgc aac tgt tcc aca acg aac atg acc tca aat caa tac agt aat
1248Ser Cys Asn Cys Ser Thr Thr Asn Met Thr Ser Asn Gln Tyr Ser Asn
405 410 415 cca acc aca aat atg acc tca aat caa tac atg atc tca cac
gag tat 1296Pro Thr Thr Asn Met Thr Ser Asn Gln Tyr Met Ile Ser His
Glu Tyr 420 425 430 acc tcg ttg ccg aat aat ttc atg ctc tca aga aat
agc aat ctg gaa 1344Thr Ser Leu Pro Asn Asn Phe Met Leu Ser Arg Asn
Ser Asn Leu Glu 435 440 445 tat aag tgt cct gaa aat aat ttc atg ata
tac tgg tac aat aat tcg 1392Tyr Lys Cys Pro Glu Asn Asn Phe Met Ile
Tyr Trp Tyr Asn Asn Ser 450 455 460 gac tgg tac aat aat tcg gat tgg
tac aat aat tga 1428Asp Trp Tyr Asn Asn Ser Asp Trp Tyr Asn Asn 465
470 475 36475PRTArtificial SequenceSynthetic Construct 36Met Ile
Ile Asp Ser Lys Thr Thr Leu Pro Arg His Ser Leu Ile His 1 5 10 15
Thr Ile Lys Leu Asn Ser Asn Lys Lys Tyr Gly Pro Gly Asp Met Thr 20
25 30 Asn Gly Asn Gln Phe Ile Ile Ser Lys Gln Glu Trp Ala Thr Ile
Gly 35 40 45 Ala Tyr Ile Gln Thr Gly Leu Gly Leu Pro Val Asn Glu
Gln Gln Leu 50 55 60 Arg Thr His Val Asn Leu Ser Gln Asp Ile Ser
Ile Pro Ser Asp Phe 65 70 75 80 Ser Gln Leu Tyr Asp Val Tyr Cys Ser
Asp Lys Thr Ser Ala Glu Trp 85 90 95 Trp Asn Lys Asn Leu Tyr Pro
Leu Ile Ile Lys Ser Ala Asn Asp Ile 100 105 110 Ala Ser Tyr Gly Phe
Lys Val Ala Gly Asp Pro Ser Ile Lys Lys Asp 115 120 125 Gly Tyr Phe
Lys Lys Leu Gln Asp Glu Leu Asp Asn Ile Val Asp Asn 130 135 140 Asn
Ser Asp Asp Asp Ala Ile Ala Lys Ala Ile Lys Asp Phe Lys Ala 145 150
155 160 Arg Cys Gly Ile Leu Ile Lys Glu Ala Lys Gln Tyr Glu Glu Ala
Ala 165 170 175 Lys Asn Ile Val Thr Ser Leu Asp Gln Phe Leu His Gly
Asp Gln Lys 180 185 190 Lys Leu Glu Gly Val Ile Asn Ile Gln Lys Arg
Leu Lys Glu Val Gln 195 200 205 Thr Ala Leu Asn Gln Ala His Gly Glu
Ser Ser Pro Ala His Lys Glu 210 215 220 Leu Leu Glu Lys Val Lys Asn
Leu Lys Thr Thr Leu Glu Arg Thr Ile 225 230 235 240 Lys Ala Glu Gln
Asp Leu Glu Lys Lys Val Glu Tyr Ser Phe Leu Leu 245 250 255 Gly Pro
Leu Leu Gly Phe Val Val Tyr Glu Ile Leu Glu Asn Thr Ala 260 265 270
Val Gln His Ile Lys Asn Gln Ile Asp Glu Ile Lys Lys Gln Leu Asp 275
280 285 Ser Ala Gln His Asp Leu Asp Arg Asp Val Lys Ile Ile Gly Met
Leu 290 295 300 Asn Ser Ile Asn Thr Asp Ile Asp Asn Leu Tyr Ser Gln
Gly Gln Glu 305 310 315 320 Ala Ile Lys Val Phe Gln Lys Leu Gln Gly
Ile Trp Ala Thr Ile Gly 325 330 335 Ala Gln Ile Glu Asn Leu Arg Thr
Thr Ser Leu Gln Glu Val Gln Asp 340 345 350 Ser Asp Asp Ala Asp Glu
Ile Gln Ile Glu Leu Glu Asp Ala Ser Asp 355 360 365 Ala Trp Leu Val
Val Ala Gln Glu Ala Arg Asp Phe Thr Leu Asn Ala 370 375 380 Tyr Ser
Thr Asn Ser Arg Gln Asn Leu Pro Ile Asn Val Ile Ser Asp 385 390 395
400 Ser Cys Asn Cys Ser Thr Thr Asn Met Thr Ser Asn Gln Tyr Ser Asn
405 410 415 Pro Thr Thr Asn Met Thr Ser Asn Gln Tyr Met Ile Ser His
Glu Tyr 420 425 430 Thr Ser Leu Pro Asn Asn Phe Met Leu Ser Arg Asn
Ser Asn Leu Glu 435 440 445 Tyr Lys Cys Pro Glu Asn Asn Phe Met Ile
Tyr Trp Tyr Asn Asn Ser 450 455 460 Asp Trp Tyr Asn Asn Ser Asp Trp
Tyr Asn Asn 465 470 475 37888DNASphingobiurn
herbicidovoransCDS(1)..(888)Native DNA sequence encoding
Sphingobiurn herbicidovorans AAD1 protein 37atg cat gct gca ctg tcc
ccc ctc tcc cag cgc ttt gag cgc atc gcg 48Met His Ala Ala Leu Ser
Pro Leu Ser Gln Arg Phe Glu Arg Ile Ala 1 5 10 15 gtc cag ccg ctg
acc ggc gtc ctg ggc gcc gag atc acc ggc gtc gac 96Val Gln Pro Leu
Thr Gly Val Leu Gly Ala Glu Ile Thr Gly Val Asp 20 25 30 ctg cgc
gag ccg ctc gac gac agc acc tgg aac gaa atc ctc gac gcg 144Leu Arg
Glu Pro Leu Asp Asp Ser Thr Trp Asn Glu Ile Leu Asp Ala 35 40 45
ttc cac act tac cag gtc atc tat ttt ccc ggc cag gcg atc acc aac
192Phe His Thr Tyr Gln Val Ile Tyr Phe Pro Gly Gln Ala Ile Thr Asn
50 55 60 gaa cag cac atc gcc ttc agc cgg cgc ttc ggc ccc gtc gat
ccc gtg 240Glu Gln His Ile Ala Phe Ser Arg Arg Phe Gly Pro
Val Asp Pro Val 65 70 75 80 ccc ctg ctc aag agc atc gaa ggg tat cca
gag gtg cag atg atc cgc 288Pro Leu Leu Lys Ser Ile Glu Gly Tyr Pro
Glu Val Gln Met Ile Arg 85 90 95 cgc gaa gcc aac gaa agc ggg cgt
gtg atc ggt gat gac tgg cac acc 336Arg Glu Ala Asn Glu Ser Gly Arg
Val Ile Gly Asp Asp Trp His Thr 100 105 110 gac agc acc ttc ctg gac
gca ccg ccg gcc gcc gtg gtg atg cgc gcg 384Asp Ser Thr Phe Leu Asp
Ala Pro Pro Ala Ala Val Val Met Arg Ala 115 120 125 atc gac gtg ccc
gag cat ggc ggc gac acc ggt ttt ctg agc atg tac 432Ile Asp Val Pro
Glu His Gly Gly Asp Thr Gly Phe Leu Ser Met Tyr 130 135 140 acc gcg
tgg gag acg ctg tcg ccc acc atg cag gcc acc atc gaa ggg 480Thr Ala
Trp Glu Thr Leu Ser Pro Thr Met Gln Ala Thr Ile Glu Gly 145 150 155
160 ttg aac gta gtg cac agc gcc acg cgt gtg ttc ggc tcg ctc tac cag
528Leu Asn Val Val His Ser Ala Thr Arg Val Phe Gly Ser Leu Tyr Gln
165 170 175 gcc cag aac cgg cgc ttc agc aac acc agc gtc aag gtg atg
gac gtc 576Ala Gln Asn Arg Arg Phe Ser Asn Thr Ser Val Lys Val Met
Asp Val 180 185 190 gac gcg ggc gac cgt gaa acc gtg cac ccc ctg gtg
gtg acc cat ccg 624Asp Ala Gly Asp Arg Glu Thr Val His Pro Leu Val
Val Thr His Pro 195 200 205 ggc agc ggc cgc aag ggc ctg tac gtg aac
cag gtc tat tgc cag cgc 672Gly Ser Gly Arg Lys Gly Leu Tyr Val Asn
Gln Val Tyr Cys Gln Arg 210 215 220 atc gag ggc atg acc gat gcc gaa
agc aaa ccg ctg ctg cag ttc ctg 720Ile Glu Gly Met Thr Asp Ala Glu
Ser Lys Pro Leu Leu Gln Phe Leu 225 230 235 240 tac gag cat gcg aca
cgg ttc gat ttc acc tgc cgc gtg cgc tgg aag 768Tyr Glu His Ala Thr
Arg Phe Asp Phe Thr Cys Arg Val Arg Trp Lys 245 250 255 aag gac cag
gtc ctg gtc tgg gac aac ctg tgc acg atg cac cgg gcc 816Lys Asp Gln
Val Leu Val Trp Asp Asn Leu Cys Thr Met His Arg Ala 260 265 270 gta
ccc gac tac gcg ggc aag ttc cgc tac ctg acg cgc acc acg gtc 864Val
Pro Asp Tyr Ala Gly Lys Phe Arg Tyr Leu Thr Arg Thr Thr Val 275 280
285 ggt ggc gtg cgc ccg gcg cgc tag 888Gly Gly Val Arg Pro Ala Arg
290 295 38295PRTSphingobiurn herbicidovorans 38Met His Ala Ala Leu
Ser Pro Leu Ser Gln Arg Phe Glu Arg Ile Ala 1 5 10 15 Val Gln Pro
Leu Thr Gly Val Leu Gly Ala Glu Ile Thr Gly Val Asp 20 25 30 Leu
Arg Glu Pro Leu Asp Asp Ser Thr Trp Asn Glu Ile Leu Asp Ala 35 40
45 Phe His Thr Tyr Gln Val Ile Tyr Phe Pro Gly Gln Ala Ile Thr Asn
50 55 60 Glu Gln His Ile Ala Phe Ser Arg Arg Phe Gly Pro Val Asp
Pro Val 65 70 75 80 Pro Leu Leu Lys Ser Ile Glu Gly Tyr Pro Glu Val
Gln Met Ile Arg 85 90 95 Arg Glu Ala Asn Glu Ser Gly Arg Val Ile
Gly Asp Asp Trp His Thr 100 105 110 Asp Ser Thr Phe Leu Asp Ala Pro
Pro Ala Ala Val Val Met Arg Ala 115 120 125 Ile Asp Val Pro Glu His
Gly Gly Asp Thr Gly Phe Leu Ser Met Tyr 130 135 140 Thr Ala Trp Glu
Thr Leu Ser Pro Thr Met Gln Ala Thr Ile Glu Gly 145 150 155 160 Leu
Asn Val Val His Ser Ala Thr Arg Val Phe Gly Ser Leu Tyr Gln 165 170
175 Ala Gln Asn Arg Arg Phe Ser Asn Thr Ser Val Lys Val Met Asp Val
180 185 190 Asp Ala Gly Asp Arg Glu Thr Val His Pro Leu Val Val Thr
His Pro 195 200 205 Gly Ser Gly Arg Lys Gly Leu Tyr Val Asn Gln Val
Tyr Cys Gln Arg 210 215 220 Ile Glu Gly Met Thr Asp Ala Glu Ser Lys
Pro Leu Leu Gln Phe Leu 225 230 235 240 Tyr Glu His Ala Thr Arg Phe
Asp Phe Thr Cys Arg Val Arg Trp Lys 245 250 255 Lys Asp Gln Val Leu
Val Trp Asp Asn Leu Cys Thr Met His Arg Ala 260 265 270 Val Pro Asp
Tyr Ala Gly Lys Phe Arg Tyr Leu Thr Arg Thr Thr Val 275 280 285 Gly
Gly Val Arg Pro Ala Arg 290 295 39888DNAArtificial
SequenceSynthetic DNA sequence encoding the AAD1 protein using
codons optimized for maize and Table 1 and Table 2 sequences are
maintainedCDS(1)..(888) 39atg cac gct gca ctg tca cca ctc tca cag
cgc ttt gag aga att gcg 48Met His Ala Ala Leu Ser Pro Leu Ser Gln
Arg Phe Glu Arg Ile Ala 1 5 10 15 gtc cag ccg ctg act ggc gtc ttg
ggc gct gag atc acc ggc gtc gat 96Val Gln Pro Leu Thr Gly Val Leu
Gly Ala Glu Ile Thr Gly Val Asp 20 25 30 ctg agg gag cct ctc gac
gat tca acg tgg aac gaa att ctc gac gcg 144Leu Arg Glu Pro Leu Asp
Asp Ser Thr Trp Asn Glu Ile Leu Asp Ala 35 40 45 ttc cat act tac
caa gtc atc tat ttt ccc ggg caa gct att acc aac 192Phe His Thr Tyr
Gln Val Ile Tyr Phe Pro Gly Gln Ala Ile Thr Asn 50 55 60 gaa caa
cac atc gct ttc tct cgg cga ttc ggc ccc gtc gat cca gtg 240Glu Gln
His Ile Ala Phe Ser Arg Arg Phe Gly Pro Val Asp Pro Val 65 70 75 80
ccc tta ctc aag tct atc gaa ggc tac cca gag gtg cag atg ata aga
288Pro Leu Leu Lys Ser Ile Glu Gly Tyr Pro Glu Val Gln Met Ile Arg
85 90 95 agg gag gcc aac gaa agc ggg cgt gtg ata ggt gat gac tgg
cac act 336Arg Glu Ala Asn Glu Ser Gly Arg Val Ile Gly Asp Asp Trp
His Thr 100 105 110 gac agc aca ttc ctg gat gca ccg ccg gcc gct gtg
gtg atg agg gca 384Asp Ser Thr Phe Leu Asp Ala Pro Pro Ala Ala Val
Val Met Arg Ala 115 120 125 atc gac gtg ccc gag cac gga ggt gac act
ggt ttc ttg agt atg tac 432Ile Asp Val Pro Glu His Gly Gly Asp Thr
Gly Phe Leu Ser Met Tyr 130 135 140 act gct tgg gag acg ctt tcg cct
act atg caa gcc aca atc gag ggg 480Thr Ala Trp Glu Thr Leu Ser Pro
Thr Met Gln Ala Thr Ile Glu Gly 145 150 155 160 ttg aat gta gtt cac
agc gcc acg cgt gtg ttc gga tct ctc tat caa 528Leu Asn Val Val His
Ser Ala Thr Arg Val Phe Gly Ser Leu Tyr Gln 165 170 175 gcc caa aac
cgg cgc ttt tca aat acc tcc gtc aag gtg atg gac gtt 576Ala Gln Asn
Arg Arg Phe Ser Asn Thr Ser Val Lys Val Met Asp Val 180 185 190 gac
gcg ggc gac cgt gaa acc gtg cac cct ctt gtt gta acc cat ccg 624Asp
Ala Gly Asp Arg Glu Thr Val His Pro Leu Val Val Thr His Pro 195 200
205 ggc agt ggt cgc aag ggc cta tac gtt aac caa gtc tat tgc cag cgc
672Gly Ser Gly Arg Lys Gly Leu Tyr Val Asn Gln Val Tyr Cys Gln Arg
210 215 220 atc gag gga atg aca gac gca gag agt aag ccg ctc ctg caa
ttc ctg 720Ile Glu Gly Met Thr Asp Ala Glu Ser Lys Pro Leu Leu Gln
Phe Leu 225 230 235 240 tac gag cac gcg aca cgg ttc gat ttc acc tgc
cgc gtg cgc tgg aaa 768Tyr Glu His Ala Thr Arg Phe Asp Phe Thr Cys
Arg Val Arg Trp Lys 245 250 255 aag gat caa gtc ctt gta tgg gac aac
ctt tgt acg atg cac cgg gcc 816Lys Asp Gln Val Leu Val Trp Asp Asn
Leu Cys Thr Met His Arg Ala 260 265 270 gtt cct gac tac gcg ggc aag
ttc aga tac ctg acg agg acc acg gtc 864Val Pro Asp Tyr Ala Gly Lys
Phe Arg Tyr Leu Thr Arg Thr Thr Val 275 280 285 ggt gga gtt agg cca
gcg aga tga 888Gly Gly Val Arg Pro Ala Arg 290 295
40295PRTArtificial SequenceSynthetic Construct 40Met His Ala Ala
Leu Ser Pro Leu Ser Gln Arg Phe Glu Arg Ile Ala 1 5 10 15 Val Gln
Pro Leu Thr Gly Val Leu Gly Ala Glu Ile Thr Gly Val Asp 20 25 30
Leu Arg Glu Pro Leu Asp Asp Ser Thr Trp Asn Glu Ile Leu Asp Ala 35
40 45 Phe His Thr Tyr Gln Val Ile Tyr Phe Pro Gly Gln Ala Ile Thr
Asn 50 55 60 Glu Gln His Ile Ala Phe Ser Arg Arg Phe Gly Pro Val
Asp Pro Val 65 70 75 80 Pro Leu Leu Lys Ser Ile Glu Gly Tyr Pro Glu
Val Gln Met Ile Arg 85 90 95 Arg Glu Ala Asn Glu Ser Gly Arg Val
Ile Gly Asp Asp Trp His Thr 100 105 110 Asp Ser Thr Phe Leu Asp Ala
Pro Pro Ala Ala Val Val Met Arg Ala 115 120 125 Ile Asp Val Pro Glu
His Gly Gly Asp Thr Gly Phe Leu Ser Met Tyr 130 135 140 Thr Ala Trp
Glu Thr Leu Ser Pro Thr Met Gln Ala Thr Ile Glu Gly 145 150 155 160
Leu Asn Val Val His Ser Ala Thr Arg Val Phe Gly Ser Leu Tyr Gln 165
170 175 Ala Gln Asn Arg Arg Phe Ser Asn Thr Ser Val Lys Val Met Asp
Val 180 185 190 Asp Ala Gly Asp Arg Glu Thr Val His Pro Leu Val Val
Thr His Pro 195 200 205 Gly Ser Gly Arg Lys Gly Leu Tyr Val Asn Gln
Val Tyr Cys Gln Arg 210 215 220 Ile Glu Gly Met Thr Asp Ala Glu Ser
Lys Pro Leu Leu Gln Phe Leu 225 230 235 240 Tyr Glu His Ala Thr Arg
Phe Asp Phe Thr Cys Arg Val Arg Trp Lys 245 250 255 Lys Asp Gln Val
Leu Val Trp Asp Asn Leu Cys Thr Met His Arg Ala 260 265 270 Val Pro
Asp Tyr Ala Gly Lys Phe Arg Tyr Leu Thr Arg Thr Thr Val 275 280 285
Gly Gly Val Arg Pro Ala Arg 290 295 41888DNAArtificial
SequenceSynthetic DNA sequence encoding the AAD1 protein using
codons optimized for maize and with sequences identified in Table 2
removed and Table 1 sequences are maintainedCDS(1)..(888) 41atg cac
gct gca ctg tca cca ctc tca cag cgc ttt gag aga att gcg 48Met His
Ala Ala Leu Ser Pro Leu Ser Gln Arg Phe Glu Arg Ile Ala 1 5 10 15
gtc cag ccg ctg act ggc gtc ttg ggc gct gag atc acc ggc gtc gat
96Val Gln Pro Leu Thr Gly Val Leu Gly Ala Glu Ile Thr Gly Val Asp
20 25 30 ctg agg gag cct ctc gac gat tca acg tgg aac gaa att ctc
gac gcg 144Leu Arg Glu Pro Leu Asp Asp Ser Thr Trp Asn Glu Ile Leu
Asp Ala 35 40 45 ttc cat act tac caa gtc atc tac ttt ccc ggg caa
gct att acc aac 192Phe His Thr Tyr Gln Val Ile Tyr Phe Pro Gly Gln
Ala Ile Thr Asn 50 55 60 gaa caa cac atc gct ttc tct cgg cga ttc
ggc ccc gtc gat cca gtg 240Glu Gln His Ile Ala Phe Ser Arg Arg Phe
Gly Pro Val Asp Pro Val 65 70 75 80 ccc tta ctc aag tct atc gaa ggc
tac cca gag gtg cag atg ata aga 288Pro Leu Leu Lys Ser Ile Glu Gly
Tyr Pro Glu Val Gln Met Ile Arg 85 90 95 agg gag gcc aac gaa agc
ggg cgt gtg ata ggt gat gac tgg cac act 336Arg Glu Ala Asn Glu Ser
Gly Arg Val Ile Gly Asp Asp Trp His Thr 100 105 110 gac agc aca ttc
ctg gat gca ccg ccg gcc gct gtg gtg atg agg gca 384Asp Ser Thr Phe
Leu Asp Ala Pro Pro Ala Ala Val Val Met Arg Ala 115 120 125 atc gac
gtg ccc gag cac gga ggt gac act ggt ttc ttg agt atg tac 432Ile Asp
Val Pro Glu His Gly Gly Asp Thr Gly Phe Leu Ser Met Tyr 130 135 140
act gct tgg gag acg ctt tcg cct act atg caa gcc aca atc gag ggg
480Thr Ala Trp Glu Thr Leu Ser Pro Thr Met Gln Ala Thr Ile Glu Gly
145 150 155 160 ttg aat gta gtt cac agc gcc acg cgt gtg ttc gga tct
ctc tat caa 528Leu Asn Val Val His Ser Ala Thr Arg Val Phe Gly Ser
Leu Tyr Gln 165 170 175 gcc caa aac cgg cgc ttt tca aat acc tcc gtc
aag gtg atg gac gtt 576Ala Gln Asn Arg Arg Phe Ser Asn Thr Ser Val
Lys Val Met Asp Val 180 185 190 gac gcg ggc gac cgt gaa acc gtg cac
cct ctt gtt gta acc cat ccg 624Asp Ala Gly Asp Arg Glu Thr Val His
Pro Leu Val Val Thr His Pro 195 200 205 ggc agt ggt cgc aag ggc cta
tac gtt aac caa gtc tat tgc cag cgc 672Gly Ser Gly Arg Lys Gly Leu
Tyr Val Asn Gln Val Tyr Cys Gln Arg 210 215 220 atc gag gga atg aca
gac gca gag agt aag ccg ctc ctg caa ttc ctg 720Ile Glu Gly Met Thr
Asp Ala Glu Ser Lys Pro Leu Leu Gln Phe Leu 225 230 235 240 tac gag
cac gcg aca cgg ttc gat ttc acc tgc cgc gtg cgc tgg aaa 768Tyr Glu
His Ala Thr Arg Phe Asp Phe Thr Cys Arg Val Arg Trp Lys 245 250 255
aag gat caa gtc ctt gta tgg gac aac ctt tgt acg atg cac cgg gcc
816Lys Asp Gln Val Leu Val Trp Asp Asn Leu Cys Thr Met His Arg Ala
260 265 270 gtt cct gac tac gcg ggc aag ttc aga tac ctg acg agg acc
acg gtc 864Val Pro Asp Tyr Ala Gly Lys Phe Arg Tyr Leu Thr Arg Thr
Thr Val 275 280 285 ggt gga gtt agg cca gcg aga tga 888Gly Gly Val
Arg Pro Ala Arg 290 295 42295PRTArtificial SequenceSynthetic
Construct 42Met His Ala Ala Leu Ser Pro Leu Ser Gln Arg Phe Glu Arg
Ile Ala 1 5 10 15 Val Gln Pro Leu Thr Gly Val Leu Gly Ala Glu Ile
Thr Gly Val Asp 20 25 30 Leu Arg Glu Pro Leu Asp Asp Ser Thr Trp
Asn Glu Ile Leu Asp Ala 35 40 45 Phe His Thr Tyr Gln Val Ile Tyr
Phe Pro Gly Gln Ala Ile Thr Asn 50 55 60 Glu Gln His Ile Ala Phe
Ser Arg Arg Phe Gly Pro Val Asp Pro Val 65 70 75 80 Pro Leu Leu Lys
Ser Ile Glu Gly Tyr Pro Glu Val Gln Met Ile Arg 85 90 95 Arg Glu
Ala Asn Glu Ser Gly Arg Val Ile Gly Asp Asp Trp His Thr 100 105 110
Asp Ser Thr Phe Leu Asp Ala Pro Pro Ala Ala Val Val Met Arg Ala 115
120 125 Ile Asp Val Pro Glu His Gly Gly Asp Thr Gly Phe Leu Ser Met
Tyr 130 135 140 Thr Ala Trp Glu Thr Leu Ser Pro Thr Met Gln Ala Thr
Ile Glu Gly 145 150 155 160 Leu Asn Val Val His Ser Ala Thr Arg Val
Phe Gly Ser Leu Tyr Gln 165 170 175 Ala Gln Asn Arg Arg Phe Ser Asn
Thr Ser Val Lys Val Met Asp Val 180 185 190 Asp Ala Gly Asp Arg Glu
Thr Val His Pro Leu Val Val Thr His Pro 195 200 205 Gly Ser Gly Arg
Lys Gly Leu Tyr Val Asn Gln Val Tyr Cys Gln Arg 210 215 220 Ile Glu
Gly Met Thr Asp Ala Glu Ser Lys Pro Leu Leu Gln Phe Leu 225 230 235
240 Tyr Glu His Ala Thr Arg Phe Asp Phe Thr Cys Arg Val Arg Trp Lys
245
250 255 Lys Asp Gln Val Leu Val Trp Asp Asn Leu Cys Thr Met His Arg
Ala 260 265 270 Val Pro Asp Tyr Ala Gly Lys Phe Arg Tyr Leu Thr Arg
Thr Thr Val 275 280 285 Gly Gly Val Arg Pro Ala Arg 290 295
431368DNAAspergillus nidulansCDS(1)..(1368)Native DNA sequence
encoding Aspergillus nidulans delta-9 fatty acid desaturase protein
43atg tct gca cca acg gcg gac atc agg gct cgc gcc ccg gag gcc aaa
48Met Ser Ala Pro Thr Ala Asp Ile Arg Ala Arg Ala Pro Glu Ala Lys 1
5 10 15 aag gtt cac atc gct gac act gct atc aac cgc cat aac tgg tac
aag 96Lys Val His Ile Ala Asp Thr Ala Ile Asn Arg His Asn Trp Tyr
Lys 20 25 30 cat gtg aac tgg ctg aac gtt ttc ctg atc atc ggt atc
ccg ctt tat 144His Val Asn Trp Leu Asn Val Phe Leu Ile Ile Gly Ile
Pro Leu Tyr 35 40 45 ggg tgc att cag gcg ttc tgg gtg cca ctg cag
ctg aag act gcc atc 192Gly Cys Ile Gln Ala Phe Trp Val Pro Leu Gln
Leu Lys Thr Ala Ile 50 55 60 tgg gcc gtc atc tac tac ttt ttc acc
ggt ctc ggt atc aca gca ggt 240Trp Ala Val Ile Tyr Tyr Phe Phe Thr
Gly Leu Gly Ile Thr Ala Gly 65 70 75 80 tac cat cgt cta tgg gct cac
tgc tcg tac tcc gcc acc ctt cct ttg 288Tyr His Arg Leu Trp Ala His
Cys Ser Tyr Ser Ala Thr Leu Pro Leu 85 90 95 cgt atc tgg ctc gct
gcc gtt ggt ggt ggt gcc gtc gaa ggt tct atc 336Arg Ile Trp Leu Ala
Ala Val Gly Gly Gly Ala Val Glu Gly Ser Ile 100 105 110 cgc tgg tgg
gct cgt gac cac cgc gct cac cac cgc tac acc gat acc 384Arg Trp Trp
Ala Arg Asp His Arg Ala His His Arg Tyr Thr Asp Thr 115 120 125 gac
aaa gac ccg tac tcc gtt cgc aag ggt ctg ctc tac tct cac ctt 432Asp
Lys Asp Pro Tyr Ser Val Arg Lys Gly Leu Leu Tyr Ser His Leu 130 135
140 ggc tgg atg gtg atg aag cag aac cct aag cgt att ggc cgt acc gat
480Gly Trp Met Val Met Lys Gln Asn Pro Lys Arg Ile Gly Arg Thr Asp
145 150 155 160 att tcc gac ctg aac gag gac ccc gtc gtt gtc tgg cag
cac cgc aac 528Ile Ser Asp Leu Asn Glu Asp Pro Val Val Val Trp Gln
His Arg Asn 165 170 175 tac ctc aag gtc gtt ttc acg atg gga ttg gct
gtg cct atg ctt gtt 576Tyr Leu Lys Val Val Phe Thr Met Gly Leu Ala
Val Pro Met Leu Val 180 185 190 gct ggt ctt gga tgg ggt gac tgg ttg
ggc ggc ttc gtg tat gcc ggc 624Ala Gly Leu Gly Trp Gly Asp Trp Leu
Gly Gly Phe Val Tyr Ala Gly 195 200 205 att ctg cgt atc ttc ttc gtc
cag cag gcg act ttc tgc gtc aac tct 672Ile Leu Arg Ile Phe Phe Val
Gln Gln Ala Thr Phe Cys Val Asn Ser 210 215 220 ttg gcc cac tgg ctc
ggt gac cag ccc ttc gat gac cgc aac tca cct 720Leu Ala His Trp Leu
Gly Asp Gln Pro Phe Asp Asp Arg Asn Ser Pro 225 230 235 240 cgt gac
cac gtt atc acc gct ctc gtc acc ctt gga gag ggc tac cac 768Arg Asp
His Val Ile Thr Ala Leu Val Thr Leu Gly Glu Gly Tyr His 245 250 255
aac ttc cac cac gag ttc ccc tcg gac tac cgt aac gcc atc gaa tgg
816Asn Phe His His Glu Phe Pro Ser Asp Tyr Arg Asn Ala Ile Glu Trp
260 265 270 cac cag tat gat ccc acc aag tgg tcc atc tgg gcc tgg aag
cag ctt 864His Gln Tyr Asp Pro Thr Lys Trp Ser Ile Trp Ala Trp Lys
Gln Leu 275 280 285 ggt ctt gcc tac gac ctg aag aag ttc cgt gcc aac
gag att gag aag 912Gly Leu Ala Tyr Asp Leu Lys Lys Phe Arg Ala Asn
Glu Ile Glu Lys 290 295 300 ggt cgt gtc cag cag ctc cag aag aag ctt
gac cgt aag cgt gcc act 960Gly Arg Val Gln Gln Leu Gln Lys Lys Leu
Asp Arg Lys Arg Ala Thr 305 310 315 320 ctc gat tgg ggt act cct ctt
gac cag ctc ccc gtc atg gag tgg gac 1008Leu Asp Trp Gly Thr Pro Leu
Asp Gln Leu Pro Val Met Glu Trp Asp 325 330 335 gac tac gtc gag cag
gct aag aac ggc cgc ggt ctc gtg gct att gcc 1056Asp Tyr Val Glu Gln
Ala Lys Asn Gly Arg Gly Leu Val Ala Ile Ala 340 345 350 ggt gtt gtc
cac gat gtc acg gac ttc atc aaa gac cac ccc ggt ggc 1104Gly Val Val
His Asp Val Thr Asp Phe Ile Lys Asp His Pro Gly Gly 355 360 365 aag
gcc atg atc agc tcc ggt att ggg aag gac gcc acc gcc atg ttc 1152Lys
Ala Met Ile Ser Ser Gly Ile Gly Lys Asp Ala Thr Ala Met Phe 370 375
380 aac ggt ggt gtc tac tac cac tcc aac gcc gca cac aac ctc ctc tct
1200Asn Gly Gly Val Tyr Tyr His Ser Asn Ala Ala His Asn Leu Leu Ser
385 390 395 400 acc atg cgt gtt ggt gtt atc cgc ggc ggc tgt gaa gtc
gaa atc tgg 1248Thr Met Arg Val Gly Val Ile Arg Gly Gly Cys Glu Val
Glu Ile Trp 405 410 415 aag cgt gcc cag aag gag aac gtg gag tac gtg
cgt gat ggc tct ggc 1296Lys Arg Ala Gln Lys Glu Asn Val Glu Tyr Val
Arg Asp Gly Ser Gly 420 425 430 cag cgc gtc atc cgt gcc ggc gag cag
cca acc aag atc cca gaa ccc 1344Gln Arg Val Ile Arg Ala Gly Glu Gln
Pro Thr Lys Ile Pro Glu Pro 435 440 445 att ccc aca gcg gat gcg gcg
tga 1368Ile Pro Thr Ala Asp Ala Ala 450 455 44455PRTAspergillus
nidulans 44Met Ser Ala Pro Thr Ala Asp Ile Arg Ala Arg Ala Pro Glu
Ala Lys 1 5 10 15 Lys Val His Ile Ala Asp Thr Ala Ile Asn Arg His
Asn Trp Tyr Lys 20 25 30 His Val Asn Trp Leu Asn Val Phe Leu Ile
Ile Gly Ile Pro Leu Tyr 35 40 45 Gly Cys Ile Gln Ala Phe Trp Val
Pro Leu Gln Leu Lys Thr Ala Ile 50 55 60 Trp Ala Val Ile Tyr Tyr
Phe Phe Thr Gly Leu Gly Ile Thr Ala Gly 65 70 75 80 Tyr His Arg Leu
Trp Ala His Cys Ser Tyr Ser Ala Thr Leu Pro Leu 85 90 95 Arg Ile
Trp Leu Ala Ala Val Gly Gly Gly Ala Val Glu Gly Ser Ile 100 105 110
Arg Trp Trp Ala Arg Asp His Arg Ala His His Arg Tyr Thr Asp Thr 115
120 125 Asp Lys Asp Pro Tyr Ser Val Arg Lys Gly Leu Leu Tyr Ser His
Leu 130 135 140 Gly Trp Met Val Met Lys Gln Asn Pro Lys Arg Ile Gly
Arg Thr Asp 145 150 155 160 Ile Ser Asp Leu Asn Glu Asp Pro Val Val
Val Trp Gln His Arg Asn 165 170 175 Tyr Leu Lys Val Val Phe Thr Met
Gly Leu Ala Val Pro Met Leu Val 180 185 190 Ala Gly Leu Gly Trp Gly
Asp Trp Leu Gly Gly Phe Val Tyr Ala Gly 195 200 205 Ile Leu Arg Ile
Phe Phe Val Gln Gln Ala Thr Phe Cys Val Asn Ser 210 215 220 Leu Ala
His Trp Leu Gly Asp Gln Pro Phe Asp Asp Arg Asn Ser Pro 225 230 235
240 Arg Asp His Val Ile Thr Ala Leu Val Thr Leu Gly Glu Gly Tyr His
245 250 255 Asn Phe His His Glu Phe Pro Ser Asp Tyr Arg Asn Ala Ile
Glu Trp 260 265 270 His Gln Tyr Asp Pro Thr Lys Trp Ser Ile Trp Ala
Trp Lys Gln Leu 275 280 285 Gly Leu Ala Tyr Asp Leu Lys Lys Phe Arg
Ala Asn Glu Ile Glu Lys 290 295 300 Gly Arg Val Gln Gln Leu Gln Lys
Lys Leu Asp Arg Lys Arg Ala Thr 305 310 315 320 Leu Asp Trp Gly Thr
Pro Leu Asp Gln Leu Pro Val Met Glu Trp Asp 325 330 335 Asp Tyr Val
Glu Gln Ala Lys Asn Gly Arg Gly Leu Val Ala Ile Ala 340 345 350 Gly
Val Val His Asp Val Thr Asp Phe Ile Lys Asp His Pro Gly Gly 355 360
365 Lys Ala Met Ile Ser Ser Gly Ile Gly Lys Asp Ala Thr Ala Met Phe
370 375 380 Asn Gly Gly Val Tyr Tyr His Ser Asn Ala Ala His Asn Leu
Leu Ser 385 390 395 400 Thr Met Arg Val Gly Val Ile Arg Gly Gly Cys
Glu Val Glu Ile Trp 405 410 415 Lys Arg Ala Gln Lys Glu Asn Val Glu
Tyr Val Arg Asp Gly Ser Gly 420 425 430 Gln Arg Val Ile Arg Ala Gly
Glu Gln Pro Thr Lys Ile Pro Glu Pro 435 440 445 Ile Pro Thr Ala Asp
Ala Ala 450 455 451368DNAArtificial SequenceSynthetic DNA sequence
encoding Aspergillus nidulans delta-9 fatty acid desaturase protein
using codons optimized for maize and Table 1 & Table 2
sequences are maintainedCDS(1)..(1368) 45atg agt gca cca acg gcg
gac ata agg gcg cgc gcc ccg gag gca aaa 48Met Ser Ala Pro Thr Ala
Asp Ile Arg Ala Arg Ala Pro Glu Ala Lys 1 5 10 15 aag gtt cac att
gct gac act gct atc aat cgc cat aac tgg tat aag 96Lys Val His Ile
Ala Asp Thr Ala Ile Asn Arg His Asn Trp Tyr Lys 20 25 30 cat gtg
aat tgg ctg aac gtt ttt ctg atc atc ggc atc ccg ctt tat 144His Val
Asn Trp Leu Asn Val Phe Leu Ile Ile Gly Ile Pro Leu Tyr 35 40 45
ggg tgt att caa gcg ttc tgg gtg cca ctc cag ctc aag act gcc atc
192Gly Cys Ile Gln Ala Phe Trp Val Pro Leu Gln Leu Lys Thr Ala Ile
50 55 60 tgg gcc gta atc tac tac ttc ttt acc ggt ttg gga atc aca
gcg ggt 240Trp Ala Val Ile Tyr Tyr Phe Phe Thr Gly Leu Gly Ile Thr
Ala Gly 65 70 75 80 tat cac aga ttg tgg gca cac tgc tcg tac tcc gcc
acc ctt cct tta 288Tyr His Arg Leu Trp Ala His Cys Ser Tyr Ser Ala
Thr Leu Pro Leu 85 90 95 cgt ata tgg ctc gct gcc gta gga gga ggc
gcc gtc gaa ggt tca atc 336Arg Ile Trp Leu Ala Ala Val Gly Gly Gly
Ala Val Glu Gly Ser Ile 100 105 110 cgt tgg tgg gct aga gac cat cgt
gct cat cat aga tat acc gat aca 384Arg Trp Trp Ala Arg Asp His Arg
Ala His His Arg Tyr Thr Asp Thr 115 120 125 gac aaa gac ccg tac tcc
gtt cgc aag ggg ctg cta tac tct cac ctt 432Asp Lys Asp Pro Tyr Ser
Val Arg Lys Gly Leu Leu Tyr Ser His Leu 130 135 140 ggc tgg atg gtg
atg aag cag aac cct aag cgt att ggc aga acc gat 480Gly Trp Met Val
Met Lys Gln Asn Pro Lys Arg Ile Gly Arg Thr Asp 145 150 155 160 att
tcc gac ctg aac gag gac ccc gtc gtt gtc tgg cag cac cgg aac 528Ile
Ser Asp Leu Asn Glu Asp Pro Val Val Val Trp Gln His Arg Asn 165 170
175 tac ctc aag gtc gtt ttc acg atg gga ttg gct gtg cct atg ctt gtt
576Tyr Leu Lys Val Val Phe Thr Met Gly Leu Ala Val Pro Met Leu Val
180 185 190 gct ggg ctt ggc tgg gga gac tgg ttg ggc ggc ttc gtg tat
gcc ggc 624Ala Gly Leu Gly Trp Gly Asp Trp Leu Gly Gly Phe Val Tyr
Ala Gly 195 200 205 ata ctg aga atc ttt ttc gtc cag caa gcg act ttt
tgc gtc aac tct 672Ile Leu Arg Ile Phe Phe Val Gln Gln Ala Thr Phe
Cys Val Asn Ser 210 215 220 ttg gcc cac tgg ctc gga gat cag ccg ttc
gat gac cgg aac agt cct 720Leu Ala His Trp Leu Gly Asp Gln Pro Phe
Asp Asp Arg Asn Ser Pro 225 230 235 240 agg gac cac gtt atc act gct
ctc gtc acc cta gga gag ggc tac cac 768Arg Asp His Val Ile Thr Ala
Leu Val Thr Leu Gly Glu Gly Tyr His 245 250 255 aac ttc cat cac gag
ttc ccc tcg gac tac cgg aac gcc atc gaa tgg 816Asn Phe His His Glu
Phe Pro Ser Asp Tyr Arg Asn Ala Ile Glu Trp 260 265 270 cac cag tat
gat cca acg aag tgg agc atc tgg gcc tgg aag cag ctt 864His Gln Tyr
Asp Pro Thr Lys Trp Ser Ile Trp Ala Trp Lys Gln Leu 275 280 285 ggt
tta gcc tac gac ctg aag aaa ttc aga gcc aac gag att gag aaa 912Gly
Leu Ala Tyr Asp Leu Lys Lys Phe Arg Ala Asn Glu Ile Glu Lys 290 295
300 ggg cgt gtc caa cag ctg caa aag aaa ctg gac cgt aag cgg gcg act
960Gly Arg Val Gln Gln Leu Gln Lys Lys Leu Asp Arg Lys Arg Ala Thr
305 310 315 320 ctc gat tgg gga aca cct ctg gat cag ctc ccc gtc atg
gag tgg gac 1008Leu Asp Trp Gly Thr Pro Leu Asp Gln Leu Pro Val Met
Glu Trp Asp 325 330 335 gac tac gtg gag caa gca aag aac ggt cgc ggt
ctc gtg gca ata gcg 1056Asp Tyr Val Glu Gln Ala Lys Asn Gly Arg Gly
Leu Val Ala Ile Ala 340 345 350 ggc gtg gtg cac gat gtc acg gat ttc
atc aaa gat cac ccg ggg ggc 1104Gly Val Val His Asp Val Thr Asp Phe
Ile Lys Asp His Pro Gly Gly 355 360 365 aag gcc atg atc agc tcc ggg
att ggc aag gac gca acc gcc atg ttc 1152Lys Ala Met Ile Ser Ser Gly
Ile Gly Lys Asp Ala Thr Ala Met Phe 370 375 380 aat ggg gga gtc tac
tac cac agc aac gca gca cac aat ctc ttg tca 1200Asn Gly Gly Val Tyr
Tyr His Ser Asn Ala Ala His Asn Leu Leu Ser 385 390 395 400 aca atg
agg gtg ggt gtt att agg ggc ggc tgt gaa gtc gaa atc tgg 1248Thr Met
Arg Val Gly Val Ile Arg Gly Gly Cys Glu Val Glu Ile Trp 405 410 415
aag agg gcg caa aag gag aat gtg gag tac gtg cga gat ggc tct ggt
1296Lys Arg Ala Gln Lys Glu Asn Val Glu Tyr Val Arg Asp Gly Ser Gly
420 425 430 caa cgc gtg atc aga gcg ggc gag cag cca acc aag ata cca
gaa ccg 1344Gln Arg Val Ile Arg Ala Gly Glu Gln Pro Thr Lys Ile Pro
Glu Pro 435 440 445 att ccc aca gcg gat gcg gcg tag 1368Ile Pro Thr
Ala Asp Ala Ala 450 455 46455PRTArtificial SequenceSynthetic
Construct 46Met Ser Ala Pro Thr Ala Asp Ile Arg Ala Arg Ala Pro Glu
Ala Lys 1 5 10 15 Lys Val His Ile Ala Asp Thr Ala Ile Asn Arg His
Asn Trp Tyr Lys 20 25 30 His Val Asn Trp Leu Asn Val Phe Leu Ile
Ile Gly Ile Pro Leu Tyr 35 40 45 Gly Cys Ile Gln Ala Phe Trp Val
Pro Leu Gln Leu Lys Thr Ala Ile 50 55 60 Trp Ala Val Ile Tyr Tyr
Phe Phe Thr Gly Leu Gly Ile Thr Ala Gly 65 70 75 80 Tyr His Arg Leu
Trp Ala His Cys Ser Tyr Ser Ala Thr Leu Pro Leu 85 90 95 Arg Ile
Trp Leu Ala Ala Val Gly Gly Gly Ala Val Glu Gly Ser Ile 100 105 110
Arg Trp Trp Ala Arg Asp His Arg Ala His His Arg Tyr Thr Asp Thr 115
120 125 Asp Lys Asp Pro Tyr Ser Val Arg Lys Gly Leu Leu Tyr Ser His
Leu 130 135 140 Gly Trp Met Val Met Lys Gln Asn Pro Lys Arg Ile Gly
Arg Thr Asp 145 150 155 160 Ile Ser Asp Leu Asn Glu Asp Pro Val Val
Val Trp Gln His Arg Asn 165 170 175 Tyr Leu Lys Val Val Phe Thr Met
Gly Leu Ala Val Pro Met Leu Val 180 185 190 Ala Gly Leu Gly Trp Gly
Asp Trp Leu Gly Gly Phe Val Tyr Ala Gly 195 200 205 Ile Leu Arg Ile
Phe Phe Val Gln Gln Ala Thr Phe Cys
Val Asn Ser 210 215 220 Leu Ala His Trp Leu Gly Asp Gln Pro Phe Asp
Asp Arg Asn Ser Pro 225 230 235 240 Arg Asp His Val Ile Thr Ala Leu
Val Thr Leu Gly Glu Gly Tyr His 245 250 255 Asn Phe His His Glu Phe
Pro Ser Asp Tyr Arg Asn Ala Ile Glu Trp 260 265 270 His Gln Tyr Asp
Pro Thr Lys Trp Ser Ile Trp Ala Trp Lys Gln Leu 275 280 285 Gly Leu
Ala Tyr Asp Leu Lys Lys Phe Arg Ala Asn Glu Ile Glu Lys 290 295 300
Gly Arg Val Gln Gln Leu Gln Lys Lys Leu Asp Arg Lys Arg Ala Thr 305
310 315 320 Leu Asp Trp Gly Thr Pro Leu Asp Gln Leu Pro Val Met Glu
Trp Asp 325 330 335 Asp Tyr Val Glu Gln Ala Lys Asn Gly Arg Gly Leu
Val Ala Ile Ala 340 345 350 Gly Val Val His Asp Val Thr Asp Phe Ile
Lys Asp His Pro Gly Gly 355 360 365 Lys Ala Met Ile Ser Ser Gly Ile
Gly Lys Asp Ala Thr Ala Met Phe 370 375 380 Asn Gly Gly Val Tyr Tyr
His Ser Asn Ala Ala His Asn Leu Leu Ser 385 390 395 400 Thr Met Arg
Val Gly Val Ile Arg Gly Gly Cys Glu Val Glu Ile Trp 405 410 415 Lys
Arg Ala Gln Lys Glu Asn Val Glu Tyr Val Arg Asp Gly Ser Gly 420 425
430 Gln Arg Val Ile Arg Ala Gly Glu Gln Pro Thr Lys Ile Pro Glu Pro
435 440 445 Ile Pro Thr Ala Asp Ala Ala 450 455 471368DNAArtificial
SequenceSynthetic DNA sequence in accordance with the invention
encoding Aspergillus nidulans delta-9 fatty acid desaturase protein
using codons optimized for maize and with sequences identified in
Table 2 removed and Table 1 sequences are maintainedCDS(1)..(1368)
47atg agt gca cca acg gcg gac ata agg gcg cgc gcc ccg gag gca aaa
48Met Ser Ala Pro Thr Ala Asp Ile Arg Ala Arg Ala Pro Glu Ala Lys 1
5 10 15 aag gtt cac att gct gac act gct atc aat cgc cat aac tgg tat
aag 96Lys Val His Ile Ala Asp Thr Ala Ile Asn Arg His Asn Trp Tyr
Lys 20 25 30 cat gtg aat tgg ctg aac gtt ttt ctg atc atc ggc atc
ccg ctt tat 144His Val Asn Trp Leu Asn Val Phe Leu Ile Ile Gly Ile
Pro Leu Tyr 35 40 45 ggg tgt att caa gcg ttc tgg gtg cca ctc cag
ctc aag act gcc atc 192Gly Cys Ile Gln Ala Phe Trp Val Pro Leu Gln
Leu Lys Thr Ala Ile 50 55 60 tgg gcc gta atc tac tac ttc ttt acc
ggt ttg gga atc aca gcg ggt 240Trp Ala Val Ile Tyr Tyr Phe Phe Thr
Gly Leu Gly Ile Thr Ala Gly 65 70 75 80 tat cac aga ttg tgg gca cac
tgc tcg tac tcc gcc acc ctt cct tta 288Tyr His Arg Leu Trp Ala His
Cys Ser Tyr Ser Ala Thr Leu Pro Leu 85 90 95 cgt ata tgg ctc gct
gcc gta gga gga ggc gcc gtc gaa ggt tca atc 336Arg Ile Trp Leu Ala
Ala Val Gly Gly Gly Ala Val Glu Gly Ser Ile 100 105 110 cgt tgg tgg
gct aga gac cat cgt gct cat cat aga tat acc gat aca 384Arg Trp Trp
Ala Arg Asp His Arg Ala His His Arg Tyr Thr Asp Thr 115 120 125 gac
aaa gac ccg tac tcc gtt cgc aag ggg ctg cta tac tct cac ctt 432Asp
Lys Asp Pro Tyr Ser Val Arg Lys Gly Leu Leu Tyr Ser His Leu 130 135
140 ggc tgg atg gtg atg aag cag aac cct aag cgt att ggc aga acc gat
480Gly Trp Met Val Met Lys Gln Asn Pro Lys Arg Ile Gly Arg Thr Asp
145 150 155 160 att agc gac ctg aac gag gac ccc gtc gtt gtc tgg cag
cac cgg aac 528Ile Ser Asp Leu Asn Glu Asp Pro Val Val Val Trp Gln
His Arg Asn 165 170 175 tac ctc aag gtc gtt ttc acg atg gga ttg gct
gtg cct atg ctt gtt 576Tyr Leu Lys Val Val Phe Thr Met Gly Leu Ala
Val Pro Met Leu Val 180 185 190 gct ggg ctt ggc tgg gga gac tgg ttg
ggc ggc ttc gtg tat gcc ggc 624Ala Gly Leu Gly Trp Gly Asp Trp Leu
Gly Gly Phe Val Tyr Ala Gly 195 200 205 ata ctg aga atc ttt ttc gtc
cag caa gcg act ttt tgc gtc aac tct 672Ile Leu Arg Ile Phe Phe Val
Gln Gln Ala Thr Phe Cys Val Asn Ser 210 215 220 ttg gcc cac tgg ctc
gga gat cag ccg ttc gat gac cgg aac agt cct 720Leu Ala His Trp Leu
Gly Asp Gln Pro Phe Asp Asp Arg Asn Ser Pro 225 230 235 240 agg gac
cac gtt atc act gct ctc gtc acc cta gga gag ggc tac cac 768Arg Asp
His Val Ile Thr Ala Leu Val Thr Leu Gly Glu Gly Tyr His 245 250 255
aac ttc cat cac gag ttc ccc tcg gac tac cgg aac gcc atc gaa tgg
816Asn Phe His His Glu Phe Pro Ser Asp Tyr Arg Asn Ala Ile Glu Trp
260 265 270 cac cag tat gat cca acg aag tgg agc atc tgg gcc tgg aag
cag ctt 864His Gln Tyr Asp Pro Thr Lys Trp Ser Ile Trp Ala Trp Lys
Gln Leu 275 280 285 ggt tta gcc tac gac ctg aag aaa ttc aga gcc aac
gag att gag aaa 912Gly Leu Ala Tyr Asp Leu Lys Lys Phe Arg Ala Asn
Glu Ile Glu Lys 290 295 300 ggg cgt gtc caa cag ctg caa aag aaa ctg
gac cgt aag cgg gcg act 960Gly Arg Val Gln Gln Leu Gln Lys Lys Leu
Asp Arg Lys Arg Ala Thr 305 310 315 320 ctc gat tgg gga aca cct ctg
gat cag ctc ccc gtc atg gag tgg gac 1008Leu Asp Trp Gly Thr Pro Leu
Asp Gln Leu Pro Val Met Glu Trp Asp 325 330 335 gac tac gtg gag caa
gca aag aac ggt cgc ggt ctc gtg gca ata gcg 1056Asp Tyr Val Glu Gln
Ala Lys Asn Gly Arg Gly Leu Val Ala Ile Ala 340 345 350 ggc gtg gtg
cac gat gtc acg gat ttc atc aaa gat cac ccg ggg ggc 1104Gly Val Val
His Asp Val Thr Asp Phe Ile Lys Asp His Pro Gly Gly 355 360 365 aag
gcc atg atc agc tcc ggg att ggc aag gac gca acc gcc atg ttc 1152Lys
Ala Met Ile Ser Ser Gly Ile Gly Lys Asp Ala Thr Ala Met Phe 370 375
380 aat ggg gga gtc tac tac cac agc aac gca gca cac aat ctc ttg tca
1200Asn Gly Gly Val Tyr Tyr His Ser Asn Ala Ala His Asn Leu Leu Ser
385 390 395 400 aca atg agg gtg ggt gtt att agg ggc ggc tgt gaa gtc
gaa atc tgg 1248Thr Met Arg Val Gly Val Ile Arg Gly Gly Cys Glu Val
Glu Ile Trp 405 410 415 aag agg gcg caa aag gag aat gtg gag tac gtg
cga gat ggc tct ggt 1296Lys Arg Ala Gln Lys Glu Asn Val Glu Tyr Val
Arg Asp Gly Ser Gly 420 425 430 caa cgc gtg atc aga gcg ggc gag cag
cca acc aag ata cca gaa ccg 1344Gln Arg Val Ile Arg Ala Gly Glu Gln
Pro Thr Lys Ile Pro Glu Pro 435 440 445 att ccc aca gcg gat gcg gcg
tag 1368Ile Pro Thr Ala Asp Ala Ala 450 455 48455PRTArtificial
SequenceSynthetic Construct 48Met Ser Ala Pro Thr Ala Asp Ile Arg
Ala Arg Ala Pro Glu Ala Lys 1 5 10 15 Lys Val His Ile Ala Asp Thr
Ala Ile Asn Arg His Asn Trp Tyr Lys 20 25 30 His Val Asn Trp Leu
Asn Val Phe Leu Ile Ile Gly Ile Pro Leu Tyr 35 40 45 Gly Cys Ile
Gln Ala Phe Trp Val Pro Leu Gln Leu Lys Thr Ala Ile 50 55 60 Trp
Ala Val Ile Tyr Tyr Phe Phe Thr Gly Leu Gly Ile Thr Ala Gly 65 70
75 80 Tyr His Arg Leu Trp Ala His Cys Ser Tyr Ser Ala Thr Leu Pro
Leu 85 90 95 Arg Ile Trp Leu Ala Ala Val Gly Gly Gly Ala Val Glu
Gly Ser Ile 100 105 110 Arg Trp Trp Ala Arg Asp His Arg Ala His His
Arg Tyr Thr Asp Thr 115 120 125 Asp Lys Asp Pro Tyr Ser Val Arg Lys
Gly Leu Leu Tyr Ser His Leu 130 135 140 Gly Trp Met Val Met Lys Gln
Asn Pro Lys Arg Ile Gly Arg Thr Asp 145 150 155 160 Ile Ser Asp Leu
Asn Glu Asp Pro Val Val Val Trp Gln His Arg Asn 165 170 175 Tyr Leu
Lys Val Val Phe Thr Met Gly Leu Ala Val Pro Met Leu Val 180 185 190
Ala Gly Leu Gly Trp Gly Asp Trp Leu Gly Gly Phe Val Tyr Ala Gly 195
200 205 Ile Leu Arg Ile Phe Phe Val Gln Gln Ala Thr Phe Cys Val Asn
Ser 210 215 220 Leu Ala His Trp Leu Gly Asp Gln Pro Phe Asp Asp Arg
Asn Ser Pro 225 230 235 240 Arg Asp His Val Ile Thr Ala Leu Val Thr
Leu Gly Glu Gly Tyr His 245 250 255 Asn Phe His His Glu Phe Pro Ser
Asp Tyr Arg Asn Ala Ile Glu Trp 260 265 270 His Gln Tyr Asp Pro Thr
Lys Trp Ser Ile Trp Ala Trp Lys Gln Leu 275 280 285 Gly Leu Ala Tyr
Asp Leu Lys Lys Phe Arg Ala Asn Glu Ile Glu Lys 290 295 300 Gly Arg
Val Gln Gln Leu Gln Lys Lys Leu Asp Arg Lys Arg Ala Thr 305 310 315
320 Leu Asp Trp Gly Thr Pro Leu Asp Gln Leu Pro Val Met Glu Trp Asp
325 330 335 Asp Tyr Val Glu Gln Ala Lys Asn Gly Arg Gly Leu Val Ala
Ile Ala 340 345 350 Gly Val Val His Asp Val Thr Asp Phe Ile Lys Asp
His Pro Gly Gly 355 360 365 Lys Ala Met Ile Ser Ser Gly Ile Gly Lys
Asp Ala Thr Ala Met Phe 370 375 380 Asn Gly Gly Val Tyr Tyr His Ser
Asn Ala Ala His Asn Leu Leu Ser 385 390 395 400 Thr Met Arg Val Gly
Val Ile Arg Gly Gly Cys Glu Val Glu Ile Trp 405 410 415 Lys Arg Ala
Gln Lys Glu Asn Val Glu Tyr Val Arg Asp Gly Ser Gly 420 425 430 Gln
Arg Val Ile Arg Ala Gly Glu Gln Pro Thr Lys Ile Pro Glu Pro 435 440
445 Ile Pro Thr Ala Asp Ala Ala 450 455 49798DNAXerophyta
viscosaCDS(1)..(798)Native DNA sequence encoding Xerophyta viscosa
SAP1 protein 49atg agg aac gag ggt ttt ctg aaa atg aag acc gac gtt
gga gtc gcc 48Met Arg Asn Glu Gly Phe Leu Lys Met Lys Thr Asp Val
Gly Val Ala 1 5 10 15 gac gag gtg atc tcc gga gat ctc aag cag ctt
ggt gac gct gca aag 96Asp Glu Val Ile Ser Gly Asp Leu Lys Gln Leu
Gly Asp Ala Ala Lys 20 25 30 cgg cta gct aaa cat gcg atc aag ctc
ggc gcc agc ttc ggg gtt ggc 144Arg Leu Ala Lys His Ala Ile Lys Leu
Gly Ala Ser Phe Gly Val Gly 35 40 45 tct acc ata gtc cag gct att
gct tcg atc gct gct atc tat ttg ttg 192Ser Thr Ile Val Gln Ala Ile
Ala Ser Ile Ala Ala Ile Tyr Leu Leu 50 55 60 ata ttg gac cgg aca
aac tgg cgt aca aat atc ttg aca tca ctt cta 240Ile Leu Asp Arg Thr
Asn Trp Arg Thr Asn Ile Leu Thr Ser Leu Leu 65 70 75 80 att cca tat
gtt tac ttg agt ctt cct tca gtg ata ttc aac cta ttc 288Ile Pro Tyr
Val Tyr Leu Ser Leu Pro Ser Val Ile Phe Asn Leu Phe 85 90 95 agg
ggc gac ctg ggc aga tgg ctt tca ttc att ggc gta gta atg aag 336Arg
Gly Asp Leu Gly Arg Trp Leu Ser Phe Ile Gly Val Val Met Lys 100 105
110 ctc ttc ttc cac cga cac ttc cca gtt acc ttg gaa ctg ctt gtg tct
384Leu Phe Phe His Arg His Phe Pro Val Thr Leu Glu Leu Leu Val Ser
115 120 125 ctc att ctc ctg att gtg gtt tcc ccc act ttc att gcc cac
aca atc 432Leu Ile Leu Leu Ile Val Val Ser Pro Thr Phe Ile Ala His
Thr Ile 130 135 140 aga ggc agt ctc att gga gtc ttc atc ttc ctt gtc
atc gcc tgc tac 480Arg Gly Ser Leu Ile Gly Val Phe Ile Phe Leu Val
Ile Ala Cys Tyr 145 150 155 160 ctc ctc caa gag cac att aga tca gct
ggt ggc ttc aaa aac gcg ttc 528Leu Leu Gln Glu His Ile Arg Ser Ala
Gly Gly Phe Lys Asn Ala Phe 165 170 175 aca aag agc aat ggg att tca
aac agc gtc ggg atc atc att cta ctg 576Thr Lys Ser Asn Gly Ile Ser
Asn Ser Val Gly Ile Ile Ile Leu Leu 180 185 190 atc cac ccg atc tgg
agc ttg gtg gtg tat ttc ctc tac acg tct ttg 624Ile His Pro Ile Trp
Ser Leu Val Val Tyr Phe Leu Tyr Thr Ser Leu 195 200 205 ctg caa ctt
ctt gca tac tct cct tcc cct tgt tgt tgc ata tta tac 672Leu Gln Leu
Leu Ala Tyr Ser Pro Ser Pro Cys Cys Cys Ile Leu Tyr 210 215 220 aat
aag tgg ttt aat ttc atg cat gtt tgt aaa tgt gta agc ctt cat 720Asn
Lys Trp Phe Asn Phe Met His Val Cys Lys Cys Val Ser Leu His 225 230
235 240 atg tat tct cag tca att ggg tca tgc gtg tcc ata ttt ttc gtg
cag 768Met Tyr Ser Gln Ser Ile Gly Ser Cys Val Ser Ile Phe Phe Val
Gln 245 250 255 ttt gta ttc atc tat gaa gct gaa ttt taa 798Phe Val
Phe Ile Tyr Glu Ala Glu Phe 260 265 50265PRTXerophyta viscosa 50Met
Arg Asn Glu Gly Phe Leu Lys Met Lys Thr Asp Val Gly Val Ala 1 5 10
15 Asp Glu Val Ile Ser Gly Asp Leu Lys Gln Leu Gly Asp Ala Ala Lys
20 25 30 Arg Leu Ala Lys His Ala Ile Lys Leu Gly Ala Ser Phe Gly
Val Gly 35 40 45 Ser Thr Ile Val Gln Ala Ile Ala Ser Ile Ala Ala
Ile Tyr Leu Leu 50 55 60 Ile Leu Asp Arg Thr Asn Trp Arg Thr Asn
Ile Leu Thr Ser Leu Leu 65 70 75 80 Ile Pro Tyr Val Tyr Leu Ser Leu
Pro Ser Val Ile Phe Asn Leu Phe 85 90 95 Arg Gly Asp Leu Gly Arg
Trp Leu Ser Phe Ile Gly Val Val Met Lys 100 105 110 Leu Phe Phe His
Arg His Phe Pro Val Thr Leu Glu Leu Leu Val Ser 115 120 125 Leu Ile
Leu Leu Ile Val Val Ser Pro Thr Phe Ile Ala His Thr Ile 130 135 140
Arg Gly Ser Leu Ile Gly Val Phe Ile Phe Leu Val Ile Ala Cys Tyr 145
150 155 160 Leu Leu Gln Glu His Ile Arg Ser Ala Gly Gly Phe Lys Asn
Ala Phe 165 170 175 Thr Lys Ser Asn Gly Ile Ser Asn Ser Val Gly Ile
Ile Ile Leu Leu 180 185 190 Ile His Pro Ile Trp Ser Leu Val Val Tyr
Phe Leu Tyr Thr Ser Leu 195 200 205 Leu Gln Leu Leu Ala Tyr Ser Pro
Ser Pro Cys Cys Cys Ile Leu Tyr 210 215 220 Asn Lys Trp Phe Asn Phe
Met His Val Cys Lys Cys Val Ser Leu His 225 230 235 240 Met Tyr Ser
Gln Ser Ile Gly Ser Cys Val Ser Ile Phe Phe Val Gln 245 250 255 Phe
Val Phe Ile Tyr Glu Ala Glu Phe 260 265 51798DNAArtificial
SequenceSynthetic DNA sequence encoding Xerophyta viscosa SAP1
protein using codons optimized for maize and Table 1 & Table 2
sequences are maintainedCDS(1)..(798) 51atg aga aac gaa ggt ttt ctg
aag atg aaa acg gac gtt ggg gtt gct 48Met Arg Asn Glu Gly Phe Leu
Lys Met Lys Thr Asp Val Gly Val Ala 1 5 10 15 gac gaa gtc atc agc
ggt gat ttg aag cag ttg ggt gat gct gcc aaa 96Asp Glu Val Ile Ser
Gly
Asp Leu Lys Gln Leu Gly Asp Ala Ala Lys 20 25 30 cgc ctt gct aag
cac gct atc aaa ctg gga gcc agc ttt ggt gtt ggt 144Arg Leu Ala Lys
His Ala Ile Lys Leu Gly Ala Ser Phe Gly Val Gly 35 40 45 tca act
atc gtt caa gcc atc gca tca ata gca gcc atc tat ctt ctg 192Ser Thr
Ile Val Gln Ala Ile Ala Ser Ile Ala Ala Ile Tyr Leu Leu 50 55 60
att ctc gat agg acc aac tgg agg acc aac atc ttg acg tcc ctc ctc
240Ile Leu Asp Arg Thr Asn Trp Arg Thr Asn Ile Leu Thr Ser Leu Leu
65 70 75 80 att ccc tac gtg tat ctg tcc ctc ccg agc gtc atc ttc aat
ctc ttt 288Ile Pro Tyr Val Tyr Leu Ser Leu Pro Ser Val Ile Phe Asn
Leu Phe 85 90 95 cgt ggg gac ctc ggg aga tgg ctg tca ttc ata ggc
gtt gtg atg aag 336Arg Gly Asp Leu Gly Arg Trp Leu Ser Phe Ile Gly
Val Val Met Lys 100 105 110 ctg ttc ttt cat agg cac ttt cct gtt act
ttg gag ctg ctt gtg agc 384Leu Phe Phe His Arg His Phe Pro Val Thr
Leu Glu Leu Leu Val Ser 115 120 125 ctc att ctt ttg att gtc gtg tca
cct acc ttc ata gct cat aca att 432Leu Ile Leu Leu Ile Val Val Ser
Pro Thr Phe Ile Ala His Thr Ile 130 135 140 cgt gga tct ttg att ggg
gtg ttc atc ttc ttg gtg ata gca tgt tat 480Arg Gly Ser Leu Ile Gly
Val Phe Ile Phe Leu Val Ile Ala Cys Tyr 145 150 155 160 ctg ctt caa
gag cac att aga tca gct ggt ggc ttc aag aac gcc ttt 528Leu Leu Gln
Glu His Ile Arg Ser Ala Gly Gly Phe Lys Asn Ala Phe 165 170 175 aca
aag tct aat gga atc tcc aac agc gtg ggc atc atc atc ctt ctg 576Thr
Lys Ser Asn Gly Ile Ser Asn Ser Val Gly Ile Ile Ile Leu Leu 180 185
190 atc cac ccg att tgg tct ctc gtc gtc tac ttc ctc tac act tca ctt
624Ile His Pro Ile Trp Ser Leu Val Val Tyr Phe Leu Tyr Thr Ser Leu
195 200 205 ctc cag ctt ttg gcc tac tca cca tcc ccg tgc tgc tgc ata
tta tac 672Leu Gln Leu Leu Ala Tyr Ser Pro Ser Pro Cys Cys Cys Ile
Leu Tyr 210 215 220 aac aag tgg ttc aac ttc atg cat gtt tgc aag tgc
gtc tct ttg cac 720Asn Lys Trp Phe Asn Phe Met His Val Cys Lys Cys
Val Ser Leu His 225 230 235 240 atg tac tct cag tcc ata ggc tca tgt
gtt tca ata ttt ttc gtc cag 768Met Tyr Ser Gln Ser Ile Gly Ser Cys
Val Ser Ile Phe Phe Val Gln 245 250 255 ttc gtg ttc atc tat gag gct
gag ttt taa 798Phe Val Phe Ile Tyr Glu Ala Glu Phe 260 265
52265PRTArtificial SequenceSynthetic Construct 52Met Arg Asn Glu
Gly Phe Leu Lys Met Lys Thr Asp Val Gly Val Ala 1 5 10 15 Asp Glu
Val Ile Ser Gly Asp Leu Lys Gln Leu Gly Asp Ala Ala Lys 20 25 30
Arg Leu Ala Lys His Ala Ile Lys Leu Gly Ala Ser Phe Gly Val Gly 35
40 45 Ser Thr Ile Val Gln Ala Ile Ala Ser Ile Ala Ala Ile Tyr Leu
Leu 50 55 60 Ile Leu Asp Arg Thr Asn Trp Arg Thr Asn Ile Leu Thr
Ser Leu Leu 65 70 75 80 Ile Pro Tyr Val Tyr Leu Ser Leu Pro Ser Val
Ile Phe Asn Leu Phe 85 90 95 Arg Gly Asp Leu Gly Arg Trp Leu Ser
Phe Ile Gly Val Val Met Lys 100 105 110 Leu Phe Phe His Arg His Phe
Pro Val Thr Leu Glu Leu Leu Val Ser 115 120 125 Leu Ile Leu Leu Ile
Val Val Ser Pro Thr Phe Ile Ala His Thr Ile 130 135 140 Arg Gly Ser
Leu Ile Gly Val Phe Ile Phe Leu Val Ile Ala Cys Tyr 145 150 155 160
Leu Leu Gln Glu His Ile Arg Ser Ala Gly Gly Phe Lys Asn Ala Phe 165
170 175 Thr Lys Ser Asn Gly Ile Ser Asn Ser Val Gly Ile Ile Ile Leu
Leu 180 185 190 Ile His Pro Ile Trp Ser Leu Val Val Tyr Phe Leu Tyr
Thr Ser Leu 195 200 205 Leu Gln Leu Leu Ala Tyr Ser Pro Ser Pro Cys
Cys Cys Ile Leu Tyr 210 215 220 Asn Lys Trp Phe Asn Phe Met His Val
Cys Lys Cys Val Ser Leu His 225 230 235 240 Met Tyr Ser Gln Ser Ile
Gly Ser Cys Val Ser Ile Phe Phe Val Gln 245 250 255 Phe Val Phe Ile
Tyr Glu Ala Glu Phe 260 265 53798DNAArtificial SequenceSynthetic
DNA sequence in accordance with the invention encoding Xerophyta
viscosa SAP1 protein using codons optimized for maize and with
sequences identified in Table 2 removed and Table 1 sequences are
maintainedCDS(1)..(798) 53atg aga aac gaa ggt ttt ctg aag atg aaa
acg gac gtt ggg gtt gct 48Met Arg Asn Glu Gly Phe Leu Lys Met Lys
Thr Asp Val Gly Val Ala 1 5 10 15 gac gaa gtc atc agc ggt gat ttg
aag cag ttg ggt gat gct gcc aaa 96Asp Glu Val Ile Ser Gly Asp Leu
Lys Gln Leu Gly Asp Ala Ala Lys 20 25 30 cgc ctt gct aag cac gct
atc aaa ctg gga gcc agc ttt ggt gtt ggt 144Arg Leu Ala Lys His Ala
Ile Lys Leu Gly Ala Ser Phe Gly Val Gly 35 40 45 tca act atc gtt
caa gcc atc gca tca ata gca gcc atc tat ctt ctg 192Ser Thr Ile Val
Gln Ala Ile Ala Ser Ile Ala Ala Ile Tyr Leu Leu 50 55 60 att ctc
gat agg acc aac tgg agg acc aac atc ttg acg tcc ctc ctc 240Ile Leu
Asp Arg Thr Asn Trp Arg Thr Asn Ile Leu Thr Ser Leu Leu 65 70 75 80
att ccc tac gtg tat ctg tcc ctc ccg agc gtc atc ttc aat ctc ttt
288Ile Pro Tyr Val Tyr Leu Ser Leu Pro Ser Val Ile Phe Asn Leu Phe
85 90 95 cgt ggg gac ctc ggg aga tgg ctg tca ttc ata ggc gtt gtg
atg aag 336Arg Gly Asp Leu Gly Arg Trp Leu Ser Phe Ile Gly Val Val
Met Lys 100 105 110 ctg ttc ttt cat agg cac ttt cct gtt act ttg gag
ctg ctt gtg agc 384Leu Phe Phe His Arg His Phe Pro Val Thr Leu Glu
Leu Leu Val Ser 115 120 125 ctc att ctt ttg att gtc gtg tct cct acc
ttc ata gct cat aca att 432Leu Ile Leu Leu Ile Val Val Ser Pro Thr
Phe Ile Ala His Thr Ile 130 135 140 cgt gga tct ttg att ggg gtg ttc
atc ttc ttg gtg ata gca tgt tat 480Arg Gly Ser Leu Ile Gly Val Phe
Ile Phe Leu Val Ile Ala Cys Tyr 145 150 155 160 ctg ctt caa gag cac
att aga tca gct ggt ggc ttc aag aac gcc ttt 528Leu Leu Gln Glu His
Ile Arg Ser Ala Gly Gly Phe Lys Asn Ala Phe 165 170 175 aca aag tct
aat gga atc tcc aac agc gtg ggc atc atc atc ctt ctg 576Thr Lys Ser
Asn Gly Ile Ser Asn Ser Val Gly Ile Ile Ile Leu Leu 180 185 190 atc
cac ccg att tgg tct ctc gtc gtc tac ttc ctc tac act tca ctt 624Ile
His Pro Ile Trp Ser Leu Val Val Tyr Phe Leu Tyr Thr Ser Leu 195 200
205 ctc cag ctt ttg gcc tac tca cca tcc cca tgc tgc tgt att ctt tac
672Leu Gln Leu Leu Ala Tyr Ser Pro Ser Pro Cys Cys Cys Ile Leu Tyr
210 215 220 aac aaa tgg ttc aac ttc atg cac gtg tgc aag tgc gtc tct
ttg cac 720Asn Lys Trp Phe Asn Phe Met His Val Cys Lys Cys Val Ser
Leu His 225 230 235 240 atg tac tct cag tcc att ggc tca tgt gtt tca
atc ttc ttt gtc cag 768Met Tyr Ser Gln Ser Ile Gly Ser Cys Val Ser
Ile Phe Phe Val Gln 245 250 255 ttc gtg ttc atc tat gag gct gag ttt
taa 798Phe Val Phe Ile Tyr Glu Ala Glu Phe 260
26554265PRTArtificial SequenceSynthetic Construct 54Met Arg Asn Glu
Gly Phe Leu Lys Met Lys Thr Asp Val Gly Val Ala 1 5 10 15 Asp Glu
Val Ile Ser Gly Asp Leu Lys Gln Leu Gly Asp Ala Ala Lys 20 25 30
Arg Leu Ala Lys His Ala Ile Lys Leu Gly Ala Ser Phe Gly Val Gly 35
40 45 Ser Thr Ile Val Gln Ala Ile Ala Ser Ile Ala Ala Ile Tyr Leu
Leu 50 55 60 Ile Leu Asp Arg Thr Asn Trp Arg Thr Asn Ile Leu Thr
Ser Leu Leu 65 70 75 80 Ile Pro Tyr Val Tyr Leu Ser Leu Pro Ser Val
Ile Phe Asn Leu Phe 85 90 95 Arg Gly Asp Leu Gly Arg Trp Leu Ser
Phe Ile Gly Val Val Met Lys 100 105 110 Leu Phe Phe His Arg His Phe
Pro Val Thr Leu Glu Leu Leu Val Ser 115 120 125 Leu Ile Leu Leu Ile
Val Val Ser Pro Thr Phe Ile Ala His Thr Ile 130 135 140 Arg Gly Ser
Leu Ile Gly Val Phe Ile Phe Leu Val Ile Ala Cys Tyr 145 150 155 160
Leu Leu Gln Glu His Ile Arg Ser Ala Gly Gly Phe Lys Asn Ala Phe 165
170 175 Thr Lys Ser Asn Gly Ile Ser Asn Ser Val Gly Ile Ile Ile Leu
Leu 180 185 190 Ile His Pro Ile Trp Ser Leu Val Val Tyr Phe Leu Tyr
Thr Ser Leu 195 200 205 Leu Gln Leu Leu Ala Tyr Ser Pro Ser Pro Cys
Cys Cys Ile Leu Tyr 210 215 220 Asn Lys Trp Phe Asn Phe Met His Val
Cys Lys Cys Val Ser Leu His 225 230 235 240 Met Tyr Ser Gln Ser Ile
Gly Ser Cys Val Ser Ile Phe Phe Val Gln 245 250 255 Phe Val Phe Ile
Tyr Glu Ala Glu Phe 260 265 55717DNAAequorea
victoriaCDS(1)..(717)Native DNA sequence encoding Aequorea victoria
GFP1 protein 55atg agt aaa gga gaa gaa ctt ttc act gga gtg gtc cca
gtt ctt gtt 48Met Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro
Val Leu Val 1 5 10 15 gaa tta gat ggc gat gtt aat ggg caa aaa ttc
tct gtc agt gga gag 96Glu Leu Asp Gly Asp Val Asn Gly Gln Lys Phe
Ser Val Ser Gly Glu 20 25 30 ggt gaa ggt gat gca aca tac gga aaa
ctt acc ctt aat ttt att tgc 144Gly Glu Gly Asp Ala Thr Tyr Gly Lys
Leu Thr Leu Asn Phe Ile Cys 35 40 45 act act ggg aag cta cct gtt
cca tgg cca aca ctt gtc act act ttc 192Thr Thr Gly Lys Leu Pro Val
Pro Trp Pro Thr Leu Val Thr Thr Phe 50 55 60 tct tat ggt gtt caa
tgc ttc tca aga tac cca gat cat atg aaa cag 240Ser Tyr Gly Val Gln
Cys Phe Ser Arg Tyr Pro Asp His Met Lys Gln 65 70 75 80 cat gac ttt
ttc aag agt gcc atg ccc gaa ggt tat gta cag gaa aga 288His Asp Phe
Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu Arg 85 90 95 act
ata ttt tac aaa gat gac ggg aac tac aag aca cgt gct gaa gtc 336Thr
Ile Phe Tyr Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu Val 100 105
110 aag ttt gaa ggt gat acc ctt gtt aat aga atc gag tta aaa ggt att
384Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly Ile
115 120 125 gat ttt aaa gaa gat gga aac att ctt gga cac aaa atg gaa
tac aac 432Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Met Glu
Tyr Asn 130 135 140 tat aac tca cat aat gta tac atc atg gga gac aaa
cca aag aat ggc 480Tyr Asn Ser His Asn Val Tyr Ile Met Gly Asp Lys
Pro Lys Asn Gly 145 150 155 160 atc aaa gtt aac ttc aaa att aga cac
aac att aaa gat gga agc gtt 528Ile Lys Val Asn Phe Lys Ile Arg His
Asn Ile Lys Asp Gly Ser Val 165 170 175 caa tta gca gac cat tat caa
caa aat act cca att ggc gat ggc cct 576Gln Leu Ala Asp His Tyr Gln
Gln Asn Thr Pro Ile Gly Asp Gly Pro 180 185 190 gtc ctt tta cca gac
aac cat tac ctg tcc aca caa tct gcc ctt tcc 624Val Leu Leu Pro Asp
Asn His Tyr Leu Ser Thr Gln Ser Ala Leu Ser 195 200 205 aaa gat ccc
aac gaa aag aga gat cac atg atc ctt ctt gag ttt gta 672Lys Asp Pro
Asn Glu Lys Arg Asp His Met Ile Leu Leu Glu Phe Val 210 215 220 aca
gct gct agg att aca cat ggc atg gat gaa cta tac aaa taa 717Thr Ala
Ala Arg Ile Thr His Gly Met Asp Glu Leu Tyr Lys 225 230 235
56238PRTAequorea victoria 56Met Ser Lys Gly Glu Glu Leu Phe Thr Gly
Val Val Pro Val Leu Val 1 5 10 15 Glu Leu Asp Gly Asp Val Asn Gly
Gln Lys Phe Ser Val Ser Gly Glu 20 25 30 Gly Glu Gly Asp Ala Thr
Tyr Gly Lys Leu Thr Leu Asn Phe Ile Cys 35 40 45 Thr Thr Gly Lys
Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr Phe 50 55 60 Ser Tyr
Gly Val Gln Cys Phe Ser Arg Tyr Pro Asp His Met Lys Gln 65 70 75 80
His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu Arg 85
90 95 Thr Ile Phe Tyr Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu
Val 100 105 110 Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu
Lys Gly Ile 115 120 125 Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His
Lys Met Glu Tyr Asn 130 135 140 Tyr Asn Ser His Asn Val Tyr Ile Met
Gly Asp Lys Pro Lys Asn Gly 145 150 155 160 Ile Lys Val Asn Phe Lys
Ile Arg His Asn Ile Lys Asp Gly Ser Val 165 170 175 Gln Leu Ala Asp
His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly Pro 180 185 190 Val Leu
Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Ala Leu Ser 195 200 205
Lys Asp Pro Asn Glu Lys Arg Asp His Met Ile Leu Leu Glu Phe Val 210
215 220 Thr Ala Ala Arg Ile Thr His Gly Met Asp Glu Leu Tyr Lys 225
230 235 57717DNAArtificial SequenceSynthetic DNA sequence encoding
Aequorea victoria GFP1 protein using codons optimized for maize and
Table 1 & Table 2 sequences are maintainedCDS(1)..(717) 57atg
agt aaa ggg gaa gaa ctt ttc acc ggc gtg gtc cca gtc ctc gtt 48Met
Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Val Leu Val 1 5 10
15 gag ttg gat ggc gat gtg aat ggg caa aaa ttc tct gtc tcc ggg gag
96Glu Leu Asp Gly Asp Val Asn Gly Gln Lys Phe Ser Val Ser Gly Glu
20 25 30 ggt gag ggt gat gca acc tac gga aag ctg acc cta aat ttt
att tgc 144Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Asn Phe
Ile Cys 35 40 45 acg act ggg aag ttg cct gtg cct tgg ccg aca ctg
gtg acg acg ttc 192Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu
Val Thr Thr Phe 50 55 60 tct tat ggt gtg cag tgt ttc tca cgc tac
ccg gat cat atg aaa cag 240Ser Tyr Gly Val Gln Cys Phe Ser Arg Tyr
Pro Asp His Met Lys Gln 65 70 75 80 cat gac ttt ttc aag tcg gcc atg
cca gaa ggc tat gta caa gag aga 288His Asp Phe Phe Lys Ser Ala Met
Pro Glu Gly Tyr Val Gln Glu Arg 85 90 95 act ata ttt tac aag gac
gac ggg aac tac aag aca cgt gct gag gtg 336Thr Ile Phe Tyr Lys Asp
Asp Gly Asn Tyr Lys Thr Arg Ala Glu Val 100 105 110 aag ttc gag ggt
gat
acc ctt gtt aat cgg atc gag cta aag ggc att 384Lys Phe Glu Gly Asp
Thr Leu Val Asn Arg Ile Glu Leu Lys Gly Ile 115 120 125 gac ttt aag
gag gac gga aac att ctg gga cac aaa atg gaa tac aac 432Asp Phe Lys
Glu Asp Gly Asn Ile Leu Gly His Lys Met Glu Tyr Asn 130 135 140 tat
aac tcg cac aac gta tac atc atg gga gac aaa cca aag aat ggc 480Tyr
Asn Ser His Asn Val Tyr Ile Met Gly Asp Lys Pro Lys Asn Gly 145 150
155 160 ata aag gtt aac ttc aag att cga cac aac att aaa gac ggc agc
gtt 528Ile Lys Val Asn Phe Lys Ile Arg His Asn Ile Lys Asp Gly Ser
Val 165 170 175 cag ttg gcc gac cac tat caa caa aat act cca att ggc
gat ggc cct 576Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly
Asp Gly Pro 180 185 190 gtc ctc tta ccc gac aac cat tac ctg tcc acg
caa tca gcg ctc agc 624Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr
Gln Ser Ala Leu Ser 195 200 205 aag gac ccc aac gag aag agg gat cac
atg atc ctc ctt gag ttt gtc 672Lys Asp Pro Asn Glu Lys Arg Asp His
Met Ile Leu Leu Glu Phe Val 210 215 220 acc gca gct agg ata acc cac
ggc atg gat gaa ctg tac aag taa 717Thr Ala Ala Arg Ile Thr His Gly
Met Asp Glu Leu Tyr Lys 225 230 235 58238PRTArtificial
SequenceSynthetic Construct 58Met Ser Lys Gly Glu Glu Leu Phe Thr
Gly Val Val Pro Val Leu Val 1 5 10 15 Glu Leu Asp Gly Asp Val Asn
Gly Gln Lys Phe Ser Val Ser Gly Glu 20 25 30 Gly Glu Gly Asp Ala
Thr Tyr Gly Lys Leu Thr Leu Asn Phe Ile Cys 35 40 45 Thr Thr Gly
Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr Phe 50 55 60 Ser
Tyr Gly Val Gln Cys Phe Ser Arg Tyr Pro Asp His Met Lys Gln 65 70
75 80 His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu
Arg 85 90 95 Thr Ile Phe Tyr Lys Asp Asp Gly Asn Tyr Lys Thr Arg
Ala Glu Val 100 105 110 Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile
Glu Leu Lys Gly Ile 115 120 125 Asp Phe Lys Glu Asp Gly Asn Ile Leu
Gly His Lys Met Glu Tyr Asn 130 135 140 Tyr Asn Ser His Asn Val Tyr
Ile Met Gly Asp Lys Pro Lys Asn Gly 145 150 155 160 Ile Lys Val Asn
Phe Lys Ile Arg His Asn Ile Lys Asp Gly Ser Val 165 170 175 Gln Leu
Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly Pro 180 185 190
Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Ala Leu Ser 195
200 205 Lys Asp Pro Asn Glu Lys Arg Asp His Met Ile Leu Leu Glu Phe
Val 210 215 220 Thr Ala Ala Arg Ile Thr His Gly Met Asp Glu Leu Tyr
Lys 225 230 235 59717DNAArtificial SequenceSynthetic DNA sequence
in accordance with the invention encoding Aequorea victoria GFP1
protein using codons optimized for maize and with sequences
identified in Table 2 removed and Table 1 sequences are
maintainedCDS(1)..(717) 59atg agt aaa ggg gaa gaa ctt ttc acc ggc
gtg gtc cca gtc ctc gtt 48Met Ser Lys Gly Glu Glu Leu Phe Thr Gly
Val Val Pro Val Leu Val1 5 10 15 gag ttg gat ggc gat gtg aat ggg
caa aaa ttc tct gtc tcc ggg gag 96Glu Leu Asp Gly Asp Val Asn Gly
Gln Lys Phe Ser Val Ser Gly Glu 20 25 30 ggt gag ggt gat gca acc
tac gga aag ctg acc cta aat ttc atc tgc 144Gly Glu Gly Asp Ala Thr
Tyr Gly Lys Leu Thr Leu Asn Phe Ile Cys 35 40 45 acg act ggg aag
ttg cct gtg cct tgg ccg aca ctg gtg acg acg ttc 192Thr Thr Gly Lys
Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr Phe 50 55 60 tct tat
ggt gtg cag tgt ttc tca cgc tac ccg gat cat atg aaa cag 240Ser Tyr
Gly Val Gln Cys Phe Ser Arg Tyr Pro Asp His Met Lys Gln 65 70 75 80
cat gac ttt ttc aag tcg gcc atg cca gaa ggc tat gta caa gag aga
288His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu Arg
85 90 95 act atc ttt tac aag gac gac ggg aac tac aag aca cgt gct
gag gtg 336Thr Ile Phe Tyr Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala
Glu Val 100 105 110 aag ttc gag ggt gat acc ctt gtt aat cgg atc gag
cta aag ggc att 384Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu
Leu Lys Gly Ile 115 120 125 gac ttt aag gag gac gga aac att ctg gga
cac aaa atg gaa tac aac 432Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly
His Lys Met Glu Tyr Asn 130 135 140 tat aac tcg cac aac gta tac atc
atg gga gac aaa cca aag aat ggc 480Tyr Asn Ser His Asn Val Tyr Ile
Met Gly Asp Lys Pro Lys Asn Gly 145 150 155 160 ata aag gtt aac ttc
aag att cga cac aac att aaa gac ggc agc gtt 528Ile Lys Val Asn Phe
Lys Ile Arg His Asn Ile Lys Asp Gly Ser Val 165 170 175 cag ttg gcc
gac cac tat caa caa aat act cca att ggc gat ggc cct 576Gln Leu Ala
Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly Pro 180 185 190 gtc
ctc tta ccc gac aac cat tac ctg tcc acg caa tca gcg ctc agc 624Val
Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Ala Leu Ser 195 200
205 aag gac ccc aac gag aag agg gat cac atg atc ctc ctt gag ttt gtc
672Lys Asp Pro Asn Glu Lys Arg Asp His Met Ile Leu Leu Glu Phe Val
210 215 220 acc gca gct agg ata acc cac ggc atg gat gaa ctg tac aag
taa 717Thr Ala Ala Arg Ile Thr His Gly Met Asp Glu Leu Tyr Lys 225
230 235 60238PRTArtificial SequenceSynthetic Construct 60Met Ser
Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Val Leu Val 1 5 10 15
Glu Leu Asp Gly Asp Val Asn Gly Gln Lys Phe Ser Val Ser Gly Glu 20
25 30 Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Asn Phe Ile
Cys 35 40 45 Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val
Thr Thr Phe 50 55 60 Ser Tyr Gly Val Gln Cys Phe Ser Arg Tyr Pro
Asp His Met Lys Gln 65 70 75 80 His Asp Phe Phe Lys Ser Ala Met Pro
Glu Gly Tyr Val Gln Glu Arg 85 90 95 Thr Ile Phe Tyr Lys Asp Asp
Gly Asn Tyr Lys Thr Arg Ala Glu Val 100 105 110 Lys Phe Glu Gly Asp
Thr Leu Val Asn Arg Ile Glu Leu Lys Gly Ile 115 120 125 Asp Phe Lys
Glu Asp Gly Asn Ile Leu Gly His Lys Met Glu Tyr Asn 130 135 140 Tyr
Asn Ser His Asn Val Tyr Ile Met Gly Asp Lys Pro Lys Asn Gly 145 150
155 160 Ile Lys Val Asn Phe Lys Ile Arg His Asn Ile Lys Asp Gly Ser
Val 165 170 175 Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly
Asp Gly Pro 180 185 190 Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr
Gln Ser Ala Leu Ser 195 200 205 Lys Asp Pro Asn Glu Lys Arg Asp His
Met Ile Leu Leu Glu Phe Val 210 215 220 Thr Ala Ala Arg Ile Thr His
Gly Met Asp Glu Leu Tyr Lys 225 230 235 611350DNALeptosphaeria
nodorumCDS(1)..(1350)Native DNA sequence encoding Leptosphaeria
nodorum delta-9 fatty acid desaturase protein 61atg gcg gcc ttg gac
agc att cca gag gat aag gct acc tcg tcg aaa 48Met Ala Ala Leu Asp
Ser Ile Pro Glu Asp Lys Ala Thr Ser Ser Lys 1 5 10 15 tcg act cat
att caa tat caa gaa gta act ttt cgg aac tgg tat aag 96Ser Thr His
Ile Gln Tyr Gln Glu Val Thr Phe Arg Asn Trp Tyr Lys 20 25 30 aag
ata aat tgg ctc aac acg acg ctg gtg gtg ctc ata ccc gct ctt 144Lys
Ile Asn Trp Leu Asn Thr Thr Leu Val Val Leu Ile Pro Ala Leu 35 40
45 gga ctc tac cta aca cgc acc acg cca ctt aca cga cct acg ctc atc
192Gly Leu Tyr Leu Thr Arg Thr Thr Pro Leu Thr Arg Pro Thr Leu Ile
50 55 60 tgg tcc gtc ctg tac tac ttc tgc aca gct ttc ggc atc aca
ggc gga 240Trp Ser Val Leu Tyr Tyr Phe Cys Thr Ala Phe Gly Ile Thr
Gly Gly 65 70 75 80 tat cat cga cta tgg agt cat cgc agc tac tcc gct
cgt cta ccg cta 288Tyr His Arg Leu Trp Ser His Arg Ser Tyr Ser Ala
Arg Leu Pro Leu 85 90 95 cgc tta ttc cta gcc ttc aca ggc gcc gga
gcc atc caa ggt agt gct 336Arg Leu Phe Leu Ala Phe Thr Gly Ala Gly
Ala Ile Gln Gly Ser Ala 100 105 110 cga tgg tgg agc gca aat cac cgc
gcc cac cac cga tgg acc gac aca 384Arg Trp Trp Ser Ala Asn His Arg
Ala His His Arg Trp Thr Asp Thr 115 120 125 atg aag gac ccc tac tcc
gtt atg cgc ggc cta tta ttc tcg cac atc 432Met Lys Asp Pro Tyr Ser
Val Met Arg Gly Leu Leu Phe Ser His Ile 130 135 140 gga tgg atg gta
ttg aac agc gac ccc aaa gtc aaa ggc cga aca gac 480Gly Trp Met Val
Leu Asn Ser Asp Pro Lys Val Lys Gly Arg Thr Asp 145 150 155 160 gtc
agt gat ctc gac agc gac ccc gtc gta gtc tgg cag cac aag cac 528Val
Ser Asp Leu Asp Ser Asp Pro Val Val Val Trp Gln His Lys His 165 170
175 tac ggc aag tgc ctg ctg ttc gcc gcg tgg ata ttc ccc atg atc gta
576Tyr Gly Lys Cys Leu Leu Phe Ala Ala Trp Ile Phe Pro Met Ile Val
180 185 190 gcc ggc ctc gga tgg gga gat tgg tgg gga ggc ctt gtc tac
gcc ggc 624Ala Gly Leu Gly Trp Gly Asp Trp Trp Gly Gly Leu Val Tyr
Ala Gly 195 200 205 atc att cga gcg tgt ttc gtc cag cag gcg aca ttt
tgc gtg aac tct 672Ile Ile Arg Ala Cys Phe Val Gln Gln Ala Thr Phe
Cys Val Asn Ser 210 215 220 ctc gcg cat tgg atc ggc gag cag ccg ttc
gac gac aga cgc acg cct 720Leu Ala His Trp Ile Gly Glu Gln Pro Phe
Asp Asp Arg Arg Thr Pro 225 230 235 240 cga gac cac gtt ttg aca gcg
ttg gta acg atg gga gaa gga tat cat 768Arg Asp His Val Leu Thr Ala
Leu Val Thr Met Gly Glu Gly Tyr His 245 250 255 aac ttc cac cac gaa
ttc cca agc gat tat cgc aac gcg atc atc tgg 816Asn Phe His His Glu
Phe Pro Ser Asp Tyr Arg Asn Ala Ile Ile Trp 260 265 270 tac caa tac
gac cct acc aaa tgg ctc att tac ctc ttc tcc ctc ggc 864Tyr Gln Tyr
Asp Pro Thr Lys Trp Leu Ile Tyr Leu Phe Ser Leu Gly 275 280 285 ccc
ttc ccc ctc gca tac tcg ctc aaa acc ttc cgg tcc aat gag att 912Pro
Phe Pro Leu Ala Tyr Ser Leu Lys Thr Phe Arg Ser Asn Glu Ile 290 295
300 gaa aaa ggg cgg ttg caa caa caa caa aaa gcc ctg gac aag aag cgc
960Glu Lys Gly Arg Leu Gln Gln Gln Gln Lys Ala Leu Asp Lys Lys Arg
305 310 315 320 tca gga ctt gat tgg ggc cta ccc ctc ttc caa ctc cct
gtc ata tcg 1008Ser Gly Leu Asp Trp Gly Leu Pro Leu Phe Gln Leu Pro
Val Ile Ser 325 330 335 tgg gac gac ttc caa gcg cgt tgc aaa gag tcc
ggc gag atg ctg gtt 1056Trp Asp Asp Phe Gln Ala Arg Cys Lys Glu Ser
Gly Glu Met Leu Val 340 345 350 gct gtc gca ggt gtg att cac gac gtc
agc cag ttt att gaa gat cac 1104Ala Val Ala Gly Val Ile His Asp Val
Ser Gln Phe Ile Glu Asp His 355 360 365 cct gga ggc agg agt ttg att
cgg agt gcg gtg ggc aaa gat ggg aca 1152Pro Gly Gly Arg Ser Leu Ile
Arg Ser Ala Val Gly Lys Asp Gly Thr 370 375 380 ggg atg ttt aat gga
ggc gta tat gag cac agt aat gcg gcg cat aat 1200Gly Met Phe Asn Gly
Gly Val Tyr Glu His Ser Asn Ala Ala His Asn 385 390 395 400 ctg ttg
tcg aca atg agg gtg gga gtg ctt aga ggt ggg cag gag gtg 1248Leu Leu
Ser Thr Met Arg Val Gly Val Leu Arg Gly Gly Gln Glu Val 405 410 415
gag gtg tgg aag aag cag aga gtg gat gtt tta ggg aag agc gac att
1296Glu Val Trp Lys Lys Gln Arg Val Asp Val Leu Gly Lys Ser Asp Ile
420 425 430 ttg aga cag gtt acg cgg gtg gag agg ttg gtt gag ggg gct
gtg gct 1344Leu Arg Gln Val Thr Arg Val Glu Arg Leu Val Glu Gly Ala
Val Ala 435 440 445 gcg tag 1350Ala 62449PRTLeptosphaeria nodorum
62Met Ala Ala Leu Asp Ser Ile Pro Glu Asp Lys Ala Thr Ser Ser Lys 1
5 10 15 Ser Thr His Ile Gln Tyr Gln Glu Val Thr Phe Arg Asn Trp Tyr
Lys 20 25 30 Lys Ile Asn Trp Leu Asn Thr Thr Leu Val Val Leu Ile
Pro Ala Leu 35 40 45 Gly Leu Tyr Leu Thr Arg Thr Thr Pro Leu Thr
Arg Pro Thr Leu Ile 50 55 60 Trp Ser Val Leu Tyr Tyr Phe Cys Thr
Ala Phe Gly Ile Thr Gly Gly 65 70 75 80 Tyr His Arg Leu Trp Ser His
Arg Ser Tyr Ser Ala Arg Leu Pro Leu 85 90 95 Arg Leu Phe Leu Ala
Phe Thr Gly Ala Gly Ala Ile Gln Gly Ser Ala 100 105 110 Arg Trp Trp
Ser Ala Asn His Arg Ala His His Arg Trp Thr Asp Thr 115 120 125 Met
Lys Asp Pro Tyr Ser Val Met Arg Gly Leu Leu Phe Ser His Ile 130 135
140 Gly Trp Met Val Leu Asn Ser Asp Pro Lys Val Lys Gly Arg Thr Asp
145 150 155 160 Val Ser Asp Leu Asp Ser Asp Pro Val Val Val Trp Gln
His Lys His 165 170 175 Tyr Gly Lys Cys Leu Leu Phe Ala Ala Trp Ile
Phe Pro Met Ile Val 180 185 190 Ala Gly Leu Gly Trp Gly Asp Trp Trp
Gly Gly Leu Val Tyr Ala Gly 195 200 205 Ile Ile Arg Ala Cys Phe Val
Gln Gln Ala Thr Phe Cys Val Asn Ser 210 215 220 Leu Ala His Trp Ile
Gly Glu Gln Pro Phe Asp Asp Arg Arg Thr Pro 225 230 235 240 Arg Asp
His Val Leu Thr Ala Leu Val Thr Met Gly Glu Gly Tyr His 245 250 255
Asn Phe His His Glu Phe Pro Ser Asp Tyr Arg Asn Ala Ile Ile Trp 260
265 270 Tyr Gln Tyr Asp Pro Thr Lys Trp Leu Ile Tyr Leu Phe Ser Leu
Gly 275 280 285 Pro Phe Pro Leu Ala Tyr Ser Leu Lys Thr Phe Arg Ser
Asn Glu Ile 290 295 300 Glu Lys Gly Arg Leu Gln Gln Gln Gln Lys Ala
Leu Asp Lys Lys Arg 305 310 315 320 Ser Gly Leu Asp Trp Gly Leu Pro
Leu Phe Gln Leu Pro Val Ile Ser 325 330 335 Trp Asp Asp Phe Gln Ala
Arg Cys Lys Glu Ser Gly Glu Met Leu Val 340 345 350 Ala Val Ala Gly
Val Ile His Asp Val Ser Gln Phe Ile Glu Asp His 355 360 365 Pro Gly
Gly Arg Ser Leu Ile Arg Ser Ala Val Gly Lys Asp Gly Thr 370 375 380
Gly Met Phe Asn Gly Gly Val Tyr Glu His Ser Asn Ala Ala His Asn 385
390 395
400 Leu Leu Ser Thr Met Arg Val Gly Val Leu Arg Gly Gly Gln Glu Val
405 410 415 Glu Val Trp Lys Lys Gln Arg Val Asp Val Leu Gly Lys Ser
Asp Ile 420 425 430 Leu Arg Gln Val Thr Arg Val Glu Arg Leu Val Glu
Gly Ala Val Ala 435 440 445 Ala 631350DNAArtificial
SequenceSynthetic DNA sequence encoding Leptosphaeria nodorum
delta-9 fatty acid desaturase protein using codons optimized for
maize and Table 1 & Table 2 sequences are
maintainedCDS(1)..(1350) 63atg gca gcc ctt gac agc atc cca gag gat
aag gct acc tcg tct aaa 48Met Ala Ala Leu Asp Ser Ile Pro Glu Asp
Lys Ala Thr Ser Ser Lys 1 5 10 15 tcg act cat att cag tac caa gaa
gtg act ttt cgg aac tgg tac aaa 96Ser Thr His Ile Gln Tyr Gln Glu
Val Thr Phe Arg Asn Trp Tyr Lys 20 25 30 aag ata aac tgg ctc aac
acg acg ctg gtg gtg ctc ata cca gct ctt 144Lys Ile Asn Trp Leu Asn
Thr Thr Leu Val Val Leu Ile Pro Ala Leu 35 40 45 ggt ctt tac cta
aca agg acc acg cca ctt act agg cca acg ctc atc 192Gly Leu Tyr Leu
Thr Arg Thr Thr Pro Leu Thr Arg Pro Thr Leu Ile 50 55 60 tgg tcc
gtc ctg tac tac ttt tgc acc gct ttc ggc att acc ggc gga 240Trp Ser
Val Leu Tyr Tyr Phe Cys Thr Ala Phe Gly Ile Thr Gly Gly 65 70 75 80
tat cat aga cta tgg agt cat cgc agc tac tcc gct cgt cta ccg ctt
288Tyr His Arg Leu Trp Ser His Arg Ser Tyr Ser Ala Arg Leu Pro Leu
85 90 95 cgc ttg ttc ctg gcc ttc act ggc gcc ggg gcc atc caa ggt
tca gct 336Arg Leu Phe Leu Ala Phe Thr Gly Ala Gly Ala Ile Gln Gly
Ser Ala 100 105 110 agg tgg tgg agc gca aat cac cgc gcc cat cat agg
tgg acc gac aca 384Arg Trp Trp Ser Ala Asn His Arg Ala His His Arg
Trp Thr Asp Thr 115 120 125 atg aag gac ccc tac tcc gtt atg cgc ggt
cta tta ttc tcg cac atc 432Met Lys Asp Pro Tyr Ser Val Met Arg Gly
Leu Leu Phe Ser His Ile 130 135 140 ggt tgg atg gtt cta aac agc gac
ccc aaa gtc aaa ggc cgc act gac 480Gly Trp Met Val Leu Asn Ser Asp
Pro Lys Val Lys Gly Arg Thr Asp 145 150 155 160 gtc tca gac cta gat
agc gac ccc gtc gtt gtc tgg cag cac aag cac 528Val Ser Asp Leu Asp
Ser Asp Pro Val Val Val Trp Gln His Lys His 165 170 175 tac ggc aag
tgc ctg cta ttt gcc gca tgg ata ttc ccg atg atc gta 576Tyr Gly Lys
Cys Leu Leu Phe Ala Ala Trp Ile Phe Pro Met Ile Val 180 185 190 gcc
ggc ctc gga tgg gga gat tgg tgg gga ggc ctt gtc tac gcc ggc 624Ala
Gly Leu Gly Trp Gly Asp Trp Trp Gly Gly Leu Val Tyr Ala Gly 195 200
205 atc att agg gcg tgt ttc gtc cag caa gca acc ttt tgc gtg aac tct
672Ile Ile Arg Ala Cys Phe Val Gln Gln Ala Thr Phe Cys Val Asn Ser
210 215 220 ctc gcg cac tgg atc ggc gag cag ccg ttc gac gac aga cgc
acc cct 720Leu Ala His Trp Ile Gly Glu Gln Pro Phe Asp Asp Arg Arg
Thr Pro 225 230 235 240 aga gac cac gtt ttg acc gcg ttg gtc act atg
gga gaa ggt tat cac 768Arg Asp His Val Leu Thr Ala Leu Val Thr Met
Gly Glu Gly Tyr His 245 250 255 aac ttc cac cac gag ttc ccg tct gat
tat agg aac gcg atc atc tgg 816Asn Phe His His Glu Phe Pro Ser Asp
Tyr Arg Asn Ala Ile Ile Trp 260 265 270 tat cag tac gac cct acc aaa
tgg ctc ata tac ctc ttc tcc ctc ggc 864Tyr Gln Tyr Asp Pro Thr Lys
Trp Leu Ile Tyr Leu Phe Ser Leu Gly 275 280 285 ccg ttc cca ctg gca
tac tcg ctc aaa acc ttc cgg tct aac gag atc 912Pro Phe Pro Leu Ala
Tyr Ser Leu Lys Thr Phe Arg Ser Asn Glu Ile 290 295 300 gaa aag ggg
cgg ttg caa caa caa caa aag gcc ctg gat aag aag cgc 960Glu Lys Gly
Arg Leu Gln Gln Gln Gln Lys Ala Leu Asp Lys Lys Arg 305 310 315 320
tct ggc ctt gat tgg ggc ctg ccc ctc ttc cag ctc cct gtg ata tct
1008Ser Gly Leu Asp Trp Gly Leu Pro Leu Phe Gln Leu Pro Val Ile Ser
325 330 335 tgg gac gac ttc caa gcg cgt tgt aag gag tcc ggc gag atg
ctg gtt 1056Trp Asp Asp Phe Gln Ala Arg Cys Lys Glu Ser Gly Glu Met
Leu Val 340 345 350 gct gtc gcc ggt gtg att cac gac gtc tca cag ttt
att gaa gat cac 1104Ala Val Ala Gly Val Ile His Asp Val Ser Gln Phe
Ile Glu Asp His 355 360 365 cct gga ggg agg agt ctg att cgg tct gcg
gtg ggc aag gat ggg act 1152Pro Gly Gly Arg Ser Leu Ile Arg Ser Ala
Val Gly Lys Asp Gly Thr 370 375 380 ggg atg ttt aat gga ggc gtt tat
gag cac agt aat gcg gcg cac aat 1200Gly Met Phe Asn Gly Gly Val Tyr
Glu His Ser Asn Ala Ala His Asn 385 390 395 400 ctg ttg tca aca atg
agg gtg ggt gtg ctt aga ggt ggg caa gag gtg 1248Leu Leu Ser Thr Met
Arg Val Gly Val Leu Arg Gly Gly Gln Glu Val 405 410 415 gag gtg tgg
aag aag cag cgt gtg gat gtt tta ggg aag agc gat atc 1296Glu Val Trp
Lys Lys Gln Arg Val Asp Val Leu Gly Lys Ser Asp Ile 420 425 430 ttg
cgt caa gtt acg cgg gtg gag agg ctg gtt gag ggg gct gtg gct 1344Leu
Arg Gln Val Thr Arg Val Glu Arg Leu Val Glu Gly Ala Val Ala 435 440
445 gcc tag 1350Ala 64449PRTArtificial SequenceSynthetic Construct
64Met Ala Ala Leu Asp Ser Ile Pro Glu Asp Lys Ala Thr Ser Ser Lys 1
5 10 15 Ser Thr His Ile Gln Tyr Gln Glu Val Thr Phe Arg Asn Trp Tyr
Lys 20 25 30 Lys Ile Asn Trp Leu Asn Thr Thr Leu Val Val Leu Ile
Pro Ala Leu 35 40 45 Gly Leu Tyr Leu Thr Arg Thr Thr Pro Leu Thr
Arg Pro Thr Leu Ile 50 55 60 Trp Ser Val Leu Tyr Tyr Phe Cys Thr
Ala Phe Gly Ile Thr Gly Gly 65 70 75 80 Tyr His Arg Leu Trp Ser His
Arg Ser Tyr Ser Ala Arg Leu Pro Leu 85 90 95 Arg Leu Phe Leu Ala
Phe Thr Gly Ala Gly Ala Ile Gln Gly Ser Ala 100 105 110 Arg Trp Trp
Ser Ala Asn His Arg Ala His His Arg Trp Thr Asp Thr 115 120 125 Met
Lys Asp Pro Tyr Ser Val Met Arg Gly Leu Leu Phe Ser His Ile 130 135
140 Gly Trp Met Val Leu Asn Ser Asp Pro Lys Val Lys Gly Arg Thr Asp
145 150 155 160 Val Ser Asp Leu Asp Ser Asp Pro Val Val Val Trp Gln
His Lys His 165 170 175 Tyr Gly Lys Cys Leu Leu Phe Ala Ala Trp Ile
Phe Pro Met Ile Val 180 185 190 Ala Gly Leu Gly Trp Gly Asp Trp Trp
Gly Gly Leu Val Tyr Ala Gly 195 200 205 Ile Ile Arg Ala Cys Phe Val
Gln Gln Ala Thr Phe Cys Val Asn Ser 210 215 220 Leu Ala His Trp Ile
Gly Glu Gln Pro Phe Asp Asp Arg Arg Thr Pro 225 230 235 240 Arg Asp
His Val Leu Thr Ala Leu Val Thr Met Gly Glu Gly Tyr His 245 250 255
Asn Phe His His Glu Phe Pro Ser Asp Tyr Arg Asn Ala Ile Ile Trp 260
265 270 Tyr Gln Tyr Asp Pro Thr Lys Trp Leu Ile Tyr Leu Phe Ser Leu
Gly 275 280 285 Pro Phe Pro Leu Ala Tyr Ser Leu Lys Thr Phe Arg Ser
Asn Glu Ile 290 295 300 Glu Lys Gly Arg Leu Gln Gln Gln Gln Lys Ala
Leu Asp Lys Lys Arg 305 310 315 320 Ser Gly Leu Asp Trp Gly Leu Pro
Leu Phe Gln Leu Pro Val Ile Ser 325 330 335 Trp Asp Asp Phe Gln Ala
Arg Cys Lys Glu Ser Gly Glu Met Leu Val 340 345 350 Ala Val Ala Gly
Val Ile His Asp Val Ser Gln Phe Ile Glu Asp His 355 360 365 Pro Gly
Gly Arg Ser Leu Ile Arg Ser Ala Val Gly Lys Asp Gly Thr 370 375 380
Gly Met Phe Asn Gly Gly Val Tyr Glu His Ser Asn Ala Ala His Asn 385
390 395 400 Leu Leu Ser Thr Met Arg Val Gly Val Leu Arg Gly Gly Gln
Glu Val 405 410 415 Glu Val Trp Lys Lys Gln Arg Val Asp Val Leu Gly
Lys Ser Asp Ile 420 425 430 Leu Arg Gln Val Thr Arg Val Glu Arg Leu
Val Glu Gly Ala Val Ala 435 440 445 Ala 651350DNAArtificial
SequenceSynthetic DNA sequence in accordance with the invention
encoding Leptosphaeria nodorum delta-9 fatty acid desaturase
protein using codons optimized for maize and with sequences
identified in Table 2 removed and Table 1 sequences are
maintainedCDS(1)..(1350) 65atg gca gcc ctt gac agc atc cca gag gat
aag gct acc tcg tct aaa 48Met Ala Ala Leu Asp Ser Ile Pro Glu Asp
Lys Ala Thr Ser Ser Lys 1 5 10 15 tcg act cat att cag tac caa gaa
gtg act ttt cgg aac tgg tac aaa 96Ser Thr His Ile Gln Tyr Gln Glu
Val Thr Phe Arg Asn Trp Tyr Lys 20 25 30 aag ata aac tgg ctc aac
acg acg ctg gtg gtg ctc ata cca gct ctt 144Lys Ile Asn Trp Leu Asn
Thr Thr Leu Val Val Leu Ile Pro Ala Leu 35 40 45 ggt ctt tac cta
aca agg acc acg cca ctt act agg cca acg ctc atc 192Gly Leu Tyr Leu
Thr Arg Thr Thr Pro Leu Thr Arg Pro Thr Leu Ile 50 55 60 tgg tcc
gtc ctg tac tac ttt tgc acc gct ttc ggc att acc ggc gga 240Trp Ser
Val Leu Tyr Tyr Phe Cys Thr Ala Phe Gly Ile Thr Gly Gly 65 70 75 80
tat cat aga cta tgg agt cat cgc agc tac tcc gct cgt cta ccg ctt
288Tyr His Arg Leu Trp Ser His Arg Ser Tyr Ser Ala Arg Leu Pro Leu
85 90 95 cgc ttg ttc ctg gcc ttc act ggc gcc ggg gcc atc caa ggt
tca gct 336Arg Leu Phe Leu Ala Phe Thr Gly Ala Gly Ala Ile Gln Gly
Ser Ala 100 105 110 agg tgg tgg agc gca aat cac cgc gcc cat cat agg
tgg acc gac aca 384Arg Trp Trp Ser Ala Asn His Arg Ala His His Arg
Trp Thr Asp Thr 115 120 125 atg aag gac ccc tac tcc gtt atg cgc ggt
ctg tta ttc tcg cac atc 432Met Lys Asp Pro Tyr Ser Val Met Arg Gly
Leu Leu Phe Ser His Ile 130 135 140 ggt tgg atg gtt cta aac agc gac
ccc aaa gtc aaa ggc cgc act gac 480Gly Trp Met Val Leu Asn Ser Asp
Pro Lys Val Lys Gly Arg Thr Asp 145 150 155 160 gtc tca gac cta gat
agc gac ccc gtc gtt gtc tgg cag cac aag cac 528Val Ser Asp Leu Asp
Ser Asp Pro Val Val Val Trp Gln His Lys His 165 170 175 tac ggc aag
tgc ctg cta ttt gcc gca tgg ata ttc ccg atg atc gta 576Tyr Gly Lys
Cys Leu Leu Phe Ala Ala Trp Ile Phe Pro Met Ile Val 180 185 190 gcc
ggc ctc gga tgg gga gat tgg tgg gga ggc ctt gtc tac gcc ggc 624Ala
Gly Leu Gly Trp Gly Asp Trp Trp Gly Gly Leu Val Tyr Ala Gly 195 200
205 atc att agg gcg tgt ttc gtc cag caa gca acc ttt tgc gtg aac tct
672Ile Ile Arg Ala Cys Phe Val Gln Gln Ala Thr Phe Cys Val Asn Ser
210 215 220 ctc gcg cac tgg atc ggc gag cag ccg ttc gac gac aga cgc
acc cct 720Leu Ala His Trp Ile Gly Glu Gln Pro Phe Asp Asp Arg Arg
Thr Pro 225 230 235 240 aga gac cac gtt ttg acc gcg ttg gtc act atg
gga gaa ggt tat cac 768Arg Asp His Val Leu Thr Ala Leu Val Thr Met
Gly Glu Gly Tyr His 245 250 255 aac ttc cac cac gag ttc ccg tct gat
tat agg aac gcg atc atc tgg 816Asn Phe His His Glu Phe Pro Ser Asp
Tyr Arg Asn Ala Ile Ile Trp 260 265 270 tat cag tac gac cct acc aaa
tgg ctc ata tac ctc ttc tcc ctc ggc 864Tyr Gln Tyr Asp Pro Thr Lys
Trp Leu Ile Tyr Leu Phe Ser Leu Gly 275 280 285 ccg ttc cca ctg gca
tac tcg ctc aaa acc ttc cgg tct aac gag atc 912Pro Phe Pro Leu Ala
Tyr Ser Leu Lys Thr Phe Arg Ser Asn Glu Ile 290 295 300 gaa aag ggg
cgg ttg caa caa caa caa aag gcc ctg gat aag aag cgc 960Glu Lys Gly
Arg Leu Gln Gln Gln Gln Lys Ala Leu Asp Lys Lys Arg 305 310 315 320
tct ggc ctt gat tgg ggc ctg ccc ctc ttc cag ctc cct gtg ata tct
1008Ser Gly Leu Asp Trp Gly Leu Pro Leu Phe Gln Leu Pro Val Ile Ser
325 330 335 tgg gac gac ttc caa gcg cgt tgt aag gag tcc ggc gag atg
ctg gtt 1056Trp Asp Asp Phe Gln Ala Arg Cys Lys Glu Ser Gly Glu Met
Leu Val 340 345 350 gct gtc gcc ggt gtg att cac gac gtc tca cag ttc
att gaa gat cac 1104Ala Val Ala Gly Val Ile His Asp Val Ser Gln Phe
Ile Glu Asp His 355 360 365 cct gga ggg agg agt ctg att cgg tct gcg
gtg ggc aag gat ggg act 1152Pro Gly Gly Arg Ser Leu Ile Arg Ser Ala
Val Gly Lys Asp Gly Thr 370 375 380 ggg atg ttt aat gga ggc gtt tat
gag cac agt aat gcg gcg cac aat 1200Gly Met Phe Asn Gly Gly Val Tyr
Glu His Ser Asn Ala Ala His Asn 385 390 395 400 ctg ttg tca aca atg
agg gtg ggt gtg ctt aga ggt ggg caa gag gtg 1248Leu Leu Ser Thr Met
Arg Val Gly Val Leu Arg Gly Gly Gln Glu Val 405 410 415 gag gtg tgg
aag aag cag cgt gtg gat gta tta ggg aag agc gat atc 1296Glu Val Trp
Lys Lys Gln Arg Val Asp Val Leu Gly Lys Ser Asp Ile 420 425 430 ttg
cgt caa gtt acg cgg gtg gag agg ctg gtt gag ggg gct gtg gct 1344Leu
Arg Gln Val Thr Arg Val Glu Arg Leu Val Glu Gly Ala Val Ala 435 440
445 gcc tag 1350Ala 66449PRTArtificial SequenceSynthetic Construct
66Met Ala Ala Leu Asp Ser Ile Pro Glu Asp Lys Ala Thr Ser Ser Lys 1
5 10 15 Ser Thr His Ile Gln Tyr Gln Glu Val Thr Phe Arg Asn Trp Tyr
Lys 20 25 30 Lys Ile Asn Trp Leu Asn Thr Thr Leu Val Val Leu Ile
Pro Ala Leu 35 40 45 Gly Leu Tyr Leu Thr Arg Thr Thr Pro Leu Thr
Arg Pro Thr Leu Ile 50 55 60 Trp Ser Val Leu Tyr Tyr Phe Cys Thr
Ala Phe Gly Ile Thr Gly Gly 65 70 75 80 Tyr His Arg Leu Trp Ser His
Arg Ser Tyr Ser Ala Arg Leu Pro Leu 85 90 95 Arg Leu Phe Leu Ala
Phe Thr Gly Ala Gly Ala Ile Gln Gly Ser Ala 100 105 110 Arg Trp Trp
Ser Ala Asn His Arg Ala His His Arg Trp Thr Asp Thr 115 120 125 Met
Lys Asp Pro Tyr Ser Val Met Arg Gly Leu Leu Phe Ser His Ile 130 135
140 Gly Trp Met Val Leu Asn Ser Asp Pro Lys Val Lys Gly Arg Thr Asp
145 150 155 160 Val Ser Asp Leu Asp Ser Asp Pro Val Val Val Trp Gln
His Lys His 165 170 175 Tyr Gly Lys Cys Leu Leu Phe Ala Ala Trp Ile
Phe Pro Met Ile Val 180 185 190 Ala Gly Leu Gly Trp Gly Asp Trp Trp
Gly Gly Leu Val Tyr Ala Gly 195 200 205 Ile Ile Arg Ala Cys Phe Val
Gln Gln Ala Thr Phe Cys Val Asn Ser 210 215
220 Leu Ala His Trp Ile Gly Glu Gln Pro Phe Asp Asp Arg Arg Thr Pro
225 230 235 240 Arg Asp His Val Leu Thr Ala Leu Val Thr Met Gly Glu
Gly Tyr His 245 250 255 Asn Phe His His Glu Phe Pro Ser Asp Tyr Arg
Asn Ala Ile Ile Trp 260 265 270 Tyr Gln Tyr Asp Pro Thr Lys Trp Leu
Ile Tyr Leu Phe Ser Leu Gly 275 280 285 Pro Phe Pro Leu Ala Tyr Ser
Leu Lys Thr Phe Arg Ser Asn Glu Ile 290 295 300 Glu Lys Gly Arg Leu
Gln Gln Gln Gln Lys Ala Leu Asp Lys Lys Arg 305 310 315 320 Ser Gly
Leu Asp Trp Gly Leu Pro Leu Phe Gln Leu Pro Val Ile Ser 325 330 335
Trp Asp Asp Phe Gln Ala Arg Cys Lys Glu Ser Gly Glu Met Leu Val 340
345 350 Ala Val Ala Gly Val Ile His Asp Val Ser Gln Phe Ile Glu Asp
His 355 360 365 Pro Gly Gly Arg Ser Leu Ile Arg Ser Ala Val Gly Lys
Asp Gly Thr 370 375 380 Gly Met Phe Asn Gly Gly Val Tyr Glu His Ser
Asn Ala Ala His Asn 385 390 395 400 Leu Leu Ser Thr Met Arg Val Gly
Val Leu Arg Gly Gly Gln Glu Val 405 410 415 Glu Val Trp Lys Lys Gln
Arg Val Asp Val Leu Gly Lys Ser Asp Ile 420 425 430 Leu Arg Gln Val
Thr Arg Val Glu Arg Leu Val Glu Gly Ala Val Ala 435 440 445 Ala
67660DNAXerophyta viscosaCDS(1)..(660)Native DNA sequence encoding
Xerophyta viscosa PER1 protein 67atg ccg ggg ctc acc att ggc gac
acg atc ccc aac ctg gag ctt gac 48Met Pro Gly Leu Thr Ile Gly Asp
Thr Ile Pro Asn Leu Glu Leu Asp 1 5 10 15 acc acc cag ggt agg atc
aaa atc cac gat tac gtc ggc aac ggc tac 96Thr Thr Gln Gly Arg Ile
Lys Ile His Asp Tyr Val Gly Asn Gly Tyr 20 25 30 gtc atc ttg ttc
tca cac cct gga gac ttc act cct gtc tgc acc acc 144Val Ile Leu Phe
Ser His Pro Gly Asp Phe Thr Pro Val Cys Thr Thr 35 40 45 gaa ctt
gga aag atg gct gct tac gcc gac gag ttc agc aag cgc ggg 192Glu Leu
Gly Lys Met Ala Ala Tyr Ala Asp Glu Phe Ser Lys Arg Gly 50 55 60
gtt aag ctt ctt ggt ctt tcc tgc gac gat gta cag agc cac aag gag
240Val Lys Leu Leu Gly Leu Ser Cys Asp Asp Val Gln Ser His Lys Glu
65 70 75 80 tgg atc aag gat atc gaa gcc tat acg ccg gga tgt cac gta
aaa tat 288Trp Ile Lys Asp Ile Glu Ala Tyr Thr Pro Gly Cys His Val
Lys Tyr 85 90 95 cct atc gcg gcg gac cca acc cgc gag att atc cag
cag cta aac atg 336Pro Ile Ala Ala Asp Pro Thr Arg Glu Ile Ile Gln
Gln Leu Asn Met 100 105 110 gta gac cca gac gag aca gag tcc agc aaa
tgc gcc gtg cct tcg cga 384Val Asp Pro Asp Glu Thr Glu Ser Ser Lys
Cys Ala Val Pro Ser Arg 115 120 125 gct ctg cac atc att ggg ccc gac
aag agg atc aag ctg agt ttc ctg 432Ala Leu His Ile Ile Gly Pro Asp
Lys Arg Ile Lys Leu Ser Phe Leu 130 135 140 tac ccc gcg tcg acg ggg
cga aac atg gat gag gtg ctg agg gca gtg 480Tyr Pro Ala Ser Thr Gly
Arg Asn Met Asp Glu Val Leu Arg Ala Val 145 150 155 160 gag tcg ctc
cag cag gcg gca aag cac aag gtg gca acg ccg gcg aac 528Glu Ser Leu
Gln Gln Ala Ala Lys His Lys Val Ala Thr Pro Ala Asn 165 170 175 tgg
aag cct ggt gaa cct gtt gtg atc aag cct gat gtg tcc agc gag 576Trp
Lys Pro Gly Glu Pro Val Val Ile Lys Pro Asp Val Ser Ser Glu 180 185
190 gag gcc aag aag ctt ttc ccg cag ggt tat aaa agt gtt gat ctt cca
624Glu Ala Lys Lys Leu Phe Pro Gln Gly Tyr Lys Ser Val Asp Leu Pro
195 200 205 tcc aag aag gat tac ctt cgt ttt acg aac gtc tga 660Ser
Lys Lys Asp Tyr Leu Arg Phe Thr Asn Val 210 215 68219PRTXerophyta
viscosa 68Met Pro Gly Leu Thr Ile Gly Asp Thr Ile Pro Asn Leu Glu
Leu Asp 1 5 10 15 Thr Thr Gln Gly Arg Ile Lys Ile His Asp Tyr Val
Gly Asn Gly Tyr 20 25 30 Val Ile Leu Phe Ser His Pro Gly Asp Phe
Thr Pro Val Cys Thr Thr 35 40 45 Glu Leu Gly Lys Met Ala Ala Tyr
Ala Asp Glu Phe Ser Lys Arg Gly 50 55 60 Val Lys Leu Leu Gly Leu
Ser Cys Asp Asp Val Gln Ser His Lys Glu 65 70 75 80 Trp Ile Lys Asp
Ile Glu Ala Tyr Thr Pro Gly Cys His Val Lys Tyr 85 90 95 Pro Ile
Ala Ala Asp Pro Thr Arg Glu Ile Ile Gln Gln Leu Asn Met 100 105 110
Val Asp Pro Asp Glu Thr Glu Ser Ser Lys Cys Ala Val Pro Ser Arg 115
120 125 Ala Leu His Ile Ile Gly Pro Asp Lys Arg Ile Lys Leu Ser Phe
Leu 130 135 140 Tyr Pro Ala Ser Thr Gly Arg Asn Met Asp Glu Val Leu
Arg Ala Val 145 150 155 160 Glu Ser Leu Gln Gln Ala Ala Lys His Lys
Val Ala Thr Pro Ala Asn 165 170 175 Trp Lys Pro Gly Glu Pro Val Val
Ile Lys Pro Asp Val Ser Ser Glu 180 185 190 Glu Ala Lys Lys Leu Phe
Pro Gln Gly Tyr Lys Ser Val Asp Leu Pro 195 200 205 Ser Lys Lys Asp
Tyr Leu Arg Phe Thr Asn Val 210 215 69660DNAArtificial
SequenceSynthetic DNA sequence encoding Xerophyta viscosa PER1
protein using codons optimized for maize and Table 1 & Table 2
sequences are maintainedCDS(1)..(660) 69atg cct gga ttg act att ggt
gac aca att ccc aac ttg gag ctg gat 48Met Pro Gly Leu Thr Ile Gly
Asp Thr Ile Pro Asn Leu Glu Leu Asp 1 5 10 15 acg aca caa ggt cgc
atc aag atc cac gac tat gtc ggg aat gga tac 96Thr Thr Gln Gly Arg
Ile Lys Ile His Asp Tyr Val Gly Asn Gly Tyr 20 25 30 gtg att ctc
ttc tca cat cct ggt gat ttc act ccg gtg tgt acc acc 144Val Ile Leu
Phe Ser His Pro Gly Asp Phe Thr Pro Val Cys Thr Thr 35 40 45 gaa
ttg ggc aag atg gct gct tat gcc gac gag ttc tct aag cgt ggt 192Glu
Leu Gly Lys Met Ala Ala Tyr Ala Asp Glu Phe Ser Lys Arg Gly 50 55
60 gtg aag ctg ctt ggg ttg tcc tgt gat gat gtc caa tca cat aag gag
240Val Lys Leu Leu Gly Leu Ser Cys Asp Asp Val Gln Ser His Lys Glu
65 70 75 80 tgg atc aaa gac ata gag gct tac aca cct ggc tgt cac gta
aaa tat 288Trp Ile Lys Asp Ile Glu Ala Tyr Thr Pro Gly Cys His Val
Lys Tyr 85 90 95 ccg att gct gct gat cca acc aga gaa atc ata cag
cag ctg aac atg 336Pro Ile Ala Ala Asp Pro Thr Arg Glu Ile Ile Gln
Gln Leu Asn Met 100 105 110 gtg gac cct gat gag acg gaa agc tct aag
tgc gct gtg cct tct agg 384Val Asp Pro Asp Glu Thr Glu Ser Ser Lys
Cys Ala Val Pro Ser Arg 115 120 125 gca ctt cac atc ata gga cca gat
aag agg atc aag ctg tcc ttc ctc 432Ala Leu His Ile Ile Gly Pro Asp
Lys Arg Ile Lys Leu Ser Phe Leu 130 135 140 tac cct gcc tct act ggt
cgc aac atg gac gaa gtt ctt aga gcc gtt 480Tyr Pro Ala Ser Thr Gly
Arg Asn Met Asp Glu Val Leu Arg Ala Val 145 150 155 160 gag tct ctt
cag caa gca gct aaa cac aaa gtt gca act cct gct aac 528Glu Ser Leu
Gln Gln Ala Ala Lys His Lys Val Ala Thr Pro Ala Asn 165 170 175 tgg
aaa cct ggc gaa cca gtc gtc atc aaa cca gac gtc agc tcc gag 576Trp
Lys Pro Gly Glu Pro Val Val Ile Lys Pro Asp Val Ser Ser Glu 180 185
190 gag gcc aag aag ctc ttt cct caa ggt tat aaa agc gtt gat ttg cct
624Glu Ala Lys Lys Leu Phe Pro Gln Gly Tyr Lys Ser Val Asp Leu Pro
195 200 205 tca aag aag gac tac ttg agg ttc acc aat gtt tga 660Ser
Lys Lys Asp Tyr Leu Arg Phe Thr Asn Val 210 215 70219PRTArtificial
SequenceSynthetic Construct 70Met Pro Gly Leu Thr Ile Gly Asp Thr
Ile Pro Asn Leu Glu Leu Asp 1 5 10 15 Thr Thr Gln Gly Arg Ile Lys
Ile His Asp Tyr Val Gly Asn Gly Tyr 20 25 30 Val Ile Leu Phe Ser
His Pro Gly Asp Phe Thr Pro Val Cys Thr Thr 35 40 45 Glu Leu Gly
Lys Met Ala Ala Tyr Ala Asp Glu Phe Ser Lys Arg Gly 50 55 60 Val
Lys Leu Leu Gly Leu Ser Cys Asp Asp Val Gln Ser His Lys Glu 65 70
75 80 Trp Ile Lys Asp Ile Glu Ala Tyr Thr Pro Gly Cys His Val Lys
Tyr 85 90 95 Pro Ile Ala Ala Asp Pro Thr Arg Glu Ile Ile Gln Gln
Leu Asn Met 100 105 110 Val Asp Pro Asp Glu Thr Glu Ser Ser Lys Cys
Ala Val Pro Ser Arg 115 120 125 Ala Leu His Ile Ile Gly Pro Asp Lys
Arg Ile Lys Leu Ser Phe Leu 130 135 140 Tyr Pro Ala Ser Thr Gly Arg
Asn Met Asp Glu Val Leu Arg Ala Val 145 150 155 160 Glu Ser Leu Gln
Gln Ala Ala Lys His Lys Val Ala Thr Pro Ala Asn 165 170 175 Trp Lys
Pro Gly Glu Pro Val Val Ile Lys Pro Asp Val Ser Ser Glu 180 185 190
Glu Ala Lys Lys Leu Phe Pro Gln Gly Tyr Lys Ser Val Asp Leu Pro 195
200 205 Ser Lys Lys Asp Tyr Leu Arg Phe Thr Asn Val 210 215
71660DNAArtificial SequenceSynthetic DNA sequence in accordance
with the invention encoding Xerophyta viscosa PER1 protein using
codons optimized for maize and with sequences identified in Table 2
removed and Table 1 sequences are maintainedCDS(1)..(660) 71atg cct
gga ttg act att ggt gac aca att ccc aac ttg gag ctg gat 48Met Pro
Gly Leu Thr Ile Gly Asp Thr Ile Pro Asn Leu Glu Leu Asp 1 5 10 15
acg aca caa ggt cgc atc aag atc cac gac tat gtc ggg aat gga tac
96Thr Thr Gln Gly Arg Ile Lys Ile His Asp Tyr Val Gly Asn Gly Tyr
20 25 30 gtg att ctc ttc tca cat cct ggt gat ttc act ccg gtg tgt
acc acc 144Val Ile Leu Phe Ser His Pro Gly Asp Phe Thr Pro Val Cys
Thr Thr 35 40 45 gaa ttg ggc aag atg gct gct tat gcc gac gag ttc
tct aag cgt ggt 192Glu Leu Gly Lys Met Ala Ala Tyr Ala Asp Glu Phe
Ser Lys Arg Gly 50 55 60 gtg aag ctg ctt ggg ttg tcc tgt gat gat
gtc caa tca cat aag gag 240Val Lys Leu Leu Gly Leu Ser Cys Asp Asp
Val Gln Ser His Lys Glu 65 70 75 80 tgg atc aaa gac ata gag gct tac
aca cct ggc tgt cac gta aaa tat 288Trp Ile Lys Asp Ile Glu Ala Tyr
Thr Pro Gly Cys His Val Lys Tyr 85 90 95 ccg att gct gct gat cca
acc aga gaa atc ata cag cag ctg aac atg 336Pro Ile Ala Ala Asp Pro
Thr Arg Glu Ile Ile Gln Gln Leu Asn Met 100 105 110 gtg gac cct gat
gag acg gaa agc tct aag tgc gct gtg cct tct agg 384Val Asp Pro Asp
Glu Thr Glu Ser Ser Lys Cys Ala Val Pro Ser Arg 115 120 125 gca ctt
cac atc ata gga cca gat aag agg atc aag ctg tcc ttc ctc 432Ala Leu
His Ile Ile Gly Pro Asp Lys Arg Ile Lys Leu Ser Phe Leu 130 135 140
tac cct gcc tct act ggt cgc aac atg gac gaa gtt ctt aga gcc gtt
480Tyr Pro Ala Ser Thr Gly Arg Asn Met Asp Glu Val Leu Arg Ala Val
145 150 155 160 gag tct ctt cag caa gca gct aaa cac aaa gtt gca act
cct gct aac 528Glu Ser Leu Gln Gln Ala Ala Lys His Lys Val Ala Thr
Pro Ala Asn 165 170 175 tgg aaa cct ggc gaa cca gtc gtc atc aaa cca
gac gtc agc tcc gag 576Trp Lys Pro Gly Glu Pro Val Val Ile Lys Pro
Asp Val Ser Ser Glu 180 185 190 gag gcc aag aag ctc ttt cct caa ggt
tat aaa agc gtt gat ttg cct 624Glu Ala Lys Lys Leu Phe Pro Gln Gly
Tyr Lys Ser Val Asp Leu Pro 195 200 205 tca aag aag gac tac ttg agg
ttc acc aat gtt tga 660Ser Lys Lys Asp Tyr Leu Arg Phe Thr Asn Val
210 215 72219PRTArtificial SequenceSynthetic Construct 72Met Pro
Gly Leu Thr Ile Gly Asp Thr Ile Pro Asn Leu Glu Leu Asp 1 5 10 15
Thr Thr Gln Gly Arg Ile Lys Ile His Asp Tyr Val Gly Asn Gly Tyr 20
25 30 Val Ile Leu Phe Ser His Pro Gly Asp Phe Thr Pro Val Cys Thr
Thr 35 40 45 Glu Leu Gly Lys Met Ala Ala Tyr Ala Asp Glu Phe Ser
Lys Arg Gly 50 55 60 Val Lys Leu Leu Gly Leu Ser Cys Asp Asp Val
Gln Ser His Lys Glu 65 70 75 80 Trp Ile Lys Asp Ile Glu Ala Tyr Thr
Pro Gly Cys His Val Lys Tyr 85 90 95 Pro Ile Ala Ala Asp Pro Thr
Arg Glu Ile Ile Gln Gln Leu Asn Met 100 105 110 Val Asp Pro Asp Glu
Thr Glu Ser Ser Lys Cys Ala Val Pro Ser Arg 115 120 125 Ala Leu His
Ile Ile Gly Pro Asp Lys Arg Ile Lys Leu Ser Phe Leu 130 135 140 Tyr
Pro Ala Ser Thr Gly Arg Asn Met Asp Glu Val Leu Arg Ala Val 145 150
155 160 Glu Ser Leu Gln Gln Ala Ala Lys His Lys Val Ala Thr Pro Ala
Asn 165 170 175 Trp Lys Pro Gly Glu Pro Val Val Ile Lys Pro Asp Val
Ser Ser Glu 180 185 190 Glu Ala Lys Lys Leu Phe Pro Gln Gly Tyr Lys
Ser Val Asp Leu Pro 195 200 205 Ser Lys Lys Asp Tyr Leu Arg Phe Thr
Asn Val 210 215
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