Srm Methods In Alzheimer's Disease And Neurological Disease Assays

Abstract

Provided herein are methods for developing selected reaction monitoring mass spectrometry (LC-SRM-MS) assays.

Description

RELATED APPLICATIONS

[0001] This application is a continuation of U.S. Ser. No. 15/355,213, filed Nov. 18, 2016, which is a continuation of U.S. application Ser. No. 14/390,447, filed Oct. 3, 2014, which is a national stage application, filed under 35 U.S.C. .sctn. 371, of International Application No. PCT/US2013/031520, filed on Mar. 14, 2013, which claims priority and benefit of U.S. Provisional Application No. 61/620,770, filed Apr. 5, 2012, the contents of each of which are incorporated by reference in their entirety.

BACKGROUND

[0002] Liquid Chromatography Selected Reaction Monitoring Mass Spectrometry (LC-SRM-MS) has emerged as an alternative technology to immunoassays for quantification of target proteins in biological samples. LC-SRM-MS methods are highly desirable because LC-SRM-MS methods provide both absolute structural specificity for the target protein and relative or absolute measurement of the target protein concentration when suitable internal standards are utilized. In contrast to immunoassays, LC-SRM-MS does not involve the manufacturing of biologics. LC-SRM-MS protein assays can be rapidly and inexpensively developed in contrast to the development of immunoassays. LC-SRM-MS are highly multiplexed, with simultaneous assays for hundreds of proteins performed in a single sample analysis. Using LC-SRM-MS in contrast to other proteomic technologies allows for complex assays for the identification diagnostic proteins in complex diseases such as cancer, autoimmune, and metabolic disease. In particular, the development of a highly multiplexed LC-SRM-MS assay that reproducibly identifies a specific set of proteins relevant to a clinical disease presents diagnostic advantages and efficiencies. To date, proteomic techniques have not enabled such assays to exist where hundreds of proteins can be accurately quantified within a single sample. The present disclosure provides accurate measurement of hundreds of Alzheimer's disease associated proteins within a single sample using multiplexed techniques.

SUMMARY

[0003] The present disclosure provides a LC-SRM-MS assay for the measurement of at least 357 Alzheimer's disease associated proteins in a single sample and in a single LC-SRM-MS assay. The assay was optimized for protein quantification and minimal interference among proteins in the assay. This LC-SRM-MS assay is novel because measurement of a large number of proteins in a single sample specifically associated with Alzheimer's disease has not been accomplished. Simultaneous measurement of such a large number of proteins without interference among the proteins requires specific techniques to distinguish among the proteins. The current disclosure provides clinical utility as this assay was used for development of Alzheimer's disease diagnostic tests for the early detection of Alzheimer's disease, managing disease treatment, as well as testing for disease recurrence.

[0004] The object of the present disclosure is to provide improved methods for the use of LC-SRM-MS in the development of assays. Accordingly, provided herein is a method for developing peptides and transitions for a plurality of at least 200 proteins for a single sample selected reaction monitoring mass spectrometry (LC-SRM-MS) assay, including the steps of providing a set of 200 or more proteins; generating transitions for each protein; determining the Mascot score for SRM-triggered tandem mass spectrometry (MS/MS) spectra; performing collision energy optimization on the transitions; selecting peptides with transitions showing the greatest peak areas of their transitions; selecting a set of transitions for each peptide, wherein the transitions for each peptide have one of the four most intense b or y transition ions; the transitions for each peptide have m/z values of at least 30 m/z above or below those of the precursor ion; the transitions for each peptide do not interfere with transitions from other peptides; and the transitions represent transitions due to breakage of peptide bond at different sites of the protein.

[0005] In one embodiment of the method, each selected peptide in the set of peptides has a monoisotopic mass of 700-5000 Da; and does not contain a cysteine or a methionine or does not contain cysteine or methionine. In other embodiments, each selected peptide contains cysteine or methionine. In another embodiment, the transitions for each peptide have one of the four most intense b or y transition ions; have m/z values of at least 30 m/z above or below those of a precursor ion; do not interfere with transitions from other peptides; and represent transitions due to breakage of peptide bond at different sites of the protein.

[0006] In another embodiment of the method, the peptides do not include any peptide that is bounded by KK, KR, RK or RR (either upstream or downstream) in the corresponding protein sequence. Specifically, the amino acid is charged at pH 7.0. In another embodiment, each peptide of said set of peptides is unique to the corresponding protein. In yet another embodiment, the peptides do not include peptides which were observed in post-translational modified forms. In still another embodiment, each set of peptides is prioritized according to one or more of the following ordered set of criteria: unique peptides first, then non-unique; peptides with no observed post-translational modifications first, then those observed with post-translational modifications; peptides within the mass range 800-3500 Da first, then those outside of 800-3500 Da; and sorted by decreasing number of variant residues. In certain embodiments, the peptides are unique in that they only appear once among the peptides run in a single assay.

[0007] In one embodiment, each set of peptides is prioritized according to all of the ordered set of criteria. In another embodiment, each prioritized set of peptides contains 1-5 peptides.

[0008] In certain embodiments of the preceding methods, the two best peptides per protein and the two best transitions per peptide are selected based on experimental data resulting from LC-SRM-MS analysis of one or more of the following experimental samples: a biological disease sample, a biological control sample, and a mixture of synthetic peptides of interest. In a particular embodiment, the biological disease and biological control samples are processed using an immunodepletion method prior to LC-SRM-MS analysis. In another embodiment, the experimental samples contain internal standard peptides. In yet another embodiment, the LC-SRM-MS analysis method specifies a maximum of 7000 transitions, including transitions of the internal standard peptides and transitions. In other embodiments the method specifies a maximum of between 1000-7000, 2000-6000, 3000-5000 and about 3500 transitions.

[0009] In one embodiment of the method, the top two transitions per peptide are selected according to one or more of the following criteria the transitions exhibit the largest peak areas measured in either of the two biological experimental samples; the transitions are not interfered with by other ions; the transitions do not exhibit an elution profile that visually differs from those of other transitions of the same peptide; or the transitions are not beyond the detection limit of both of the two biological experimental samples.

[0010] In another embodiment of the method, the top two peptides per protein are selected according to one or more of the following criteria: one or more peptides exhibit two transitions and represent the largest combined peak areas of the two transitions; or one or more peptides exhibit one transition and represent the largest combined peak areas of the two transitions.

[0011] In another aspect, provided herein is an assay developed according to the foregoing method, and embodiments thereof.

[0012] In yet another aspect provided herein is the use of an assay developed according to the foregoing method, and embodiments thereof, to detect a plurality of at least 200 proteins in a single biological sample.

[0013] In another aspect, provided herein is an assay developed according to the foregoing method, and embodiments thereof.

[0014] The disclosure provides a use of a composition, as described above, for the development of an assay to detect a disease, disorder or condition in a mammal.

[0015] The disclosure provides a method comprising analyzing a composition, as described above, using mass spectrometry. The method can use selected reaction monitoring mass spectrometry.

DETAILED DESCRIPTION

[0016] The present disclosure relates to methods for developing peptides and transitions for a single sample selected reaction monitoring mass spectrometry (LC-SRM-MS) assay, generally comprising the steps of providing a set of proteins; identifying representative proteolytic peptides for each protein according to a set of criteria; identifying representative transitions for each peptide according to another set of criteria; and selecting the optimum peptides per protein and the optimum transitions per peptide.

[0017] Selected reaction monitoring mass spectrometry is capable of highly sensitive and accurate protein quantification based on the quantification of proteolytic peptides. In terms of clinical utility, mass spectrometry-based assays are often compared to immunoassays (e.g., Enzyme-Linked Immunosorbent Assay, or ELISA), which have the ability to quantify specific analytes in large sample sets (e.g., 96 or 384 samples in parallel microtitre plate-based format). Until recently, mass spectrometry-based protein assays were not able to match these sample sizes or quantitative accuracy. Considerable time and expense is required to generate and characterize antibodies required for immunoassays. Increasingly efficient LC-SRM-MS assays, therefore, may surpass immunoassays such as ELISA in the rapid development of clinically useful, multiplexed protein assays.

[0018] LC-SRM-MS is a highly selective method of tandem mass spectrometry which has the potential to effectively filter out all molecules and contaminants except the desired analyte(s). This is particularly beneficial if the analysis sample is a complex mixture which may comprise several isobaric species within a defined analytical window. LC-SRM-MS methods may utilize a triple quadrupole mass spectrometer which, as is known in the art, includes three quadrupole rod sets. A first stage of mass selection is performed in the first quadrupole rod set, and the selectively transmitted ions are fragmented in the second quadrupole rod set. The resultant transition (product) ions are conveyed to the third quadrupole rod set, which performs a second stage of mass selection. The product ions transmitted through the third quadrupole rod set are measured by a detector, which generates a signal representative of the numbers of selectively transmitted product ions. The RF and DC potentials applied to the first and third quadrupoles are tuned to select (respectively) precursor and product ions that have m/z values lying within narrow specified ranges. By specifying the appropriate transitions (m/z values of precursor and product ions), a peptide corresponding to a targeted protein may be measured with high degrees of sensitivity and selectivity. Signal-to-noise ratio in LC_SRM_MS is often superior to conventional tandem mass spectrometry (MS/MS) experiments, that do not selectively target (filter) particular analytes but rather aim to survey all analytes in the sample.

[0019] Accordingly, provided herein is a method for developing peptides and transitions for a plurality of proteins for use in selected reaction monitoring mass spectrometry (LC-SRM-MS) assay. In a preferred embodiment, the assay involves the analysis of a single sample containing all analytes of interest (e.g., a proteolytic digest of plasma proteins). As to the selection of the protease(s) used, trypsin, which cleaves exclusively C-terminal to arginine and lysine residues, is a preferred choice to generate peptides because the masses of generated peptides are compatible with the detection ability of most mass spectrometers (up to 2000 m/z), the number and average length of generated peptides, and also the availability of efficient algorithms for the generation of databases of theoretical trypsin-generated peptides. High cleavage specificity, availability, and cost are other advantages of trypsin. Other suitable proteases will be known to those of skill in the art. Miscleavage is a factor for failure or ambiguous protein identification. A miscleavage can be defined as partial enzymatic protein cleavages generating peptides with internal missed cleavage sites reflecting the allowed number of sites (targeted amino acids) per peptide that were not cut. The presence of post-translational modifications (PTMs) is also a potential contributor to the problem of miscleavages.

[0020] LC-SRM-MS mass spectrometry involves the fragmentation of gas phase ions and occurs between the different stages of mass analysis. There are many methods used to fragment the ions and these can result in different types of fragmentation and thus different information about the structure and composition of the molecule. The transition ions observed in an LC-SRM-MS spectrum result from several different factors, which include, but are not limited to, the primary sequence, the amount of internal energy, the means of introducing the energy, and charge state. Transitions must carry at least one charge to be detected. An ion is categorized as either a, b or c if the charge is on a transition comprising the original N terminus of the peptide, whereas the ion is categorized as either x, y or z if the charge is on a transition comprising the original C terminus of the peptide. A subscript indicates the number of residues in the transition (e.g., one peptide residue in x.sub.1, two peptide residues in y.sub.2, and three peptide residues in z.sub.3, etc.).

[0021] In a generic peptide repeat unit represented --N--C(O)--C--, an x ion and an a ion resulting from cleavage of the carbonyl-carbon bond (i.e., C(O)--C). The x ion is an acylium ion, and the a ion is an iminium ion. A y ion and a b ion result from cleavage of the carbonyl-nitrogen bond (i.e., C(O)--N, also known as the amide bond). In this case, the y ion is an ammonium ion and the b ion is an acylium ion. Finally, a z ion and a c ion result from cleavage of the nitrogen-carbon (i.e., C--N) bond. The z ion is a carbocation and the c ion is an ammonium ion.

[0022] Superscripts are sometimes used to indicate neutral losses in addition to the backbone fragmentation, for example, * for loss of ammonia and .degree. for loss of water. In addition to protons, c ions and y ions may abstract an additional proton from the precursor peptide. In electrospray ionization, tryptic peptides may carry more than one charge.

[0023] Internal transitions arise from double backbone cleavage. These may be formed by a combination of b-type and y-type cleavage (i.e., cleavage producing b and y ions). Internal cleavage ions may also be formed by a combination of a-type and y-type cleavage. An internal transition with a single side chain formed by a combination of a-type and y-type cleavage is called an iminium ion (sometimes also referred to as an imonium or immonium ion). These ions are labeled with the one letter code for the corresponding amino acid.

[0024] Low energy CID (i.e., collision induced dissociation in a triple quadrupole or an ion trap) involves the fragmentation of a peptide carrying a positive charge, primarily along its backbone, to generate primarily a, b and y ions.

[0025] In one aspect, provided herein is a method for developing peptides and transitions for a plurality of proteins for a single sample selected reaction monitoring mass spectrometry (LC-SRM-MS) assay: (a) providing a panel or plurality of proteins; (b) identifying a set of peptides for each protein, wherein (i) each peptide in the set of peptides corresponds to a transition of said protein; (ii) the peptides have a monoisotopic mass of 700-5000 Da; and (iii) the peptides do not contain cysteine or does not contain cysteine or methionine. In other embodiments, each selected peptide contains cysteine or methionine. ; and; (c) identifying a set of transitions for each peptide, wherein (i) the transitions for each peptide have one of the four most intense b or y transition ions; (ii) the transitions for each peptide have m/z values of at least 30 m/z above or below those of the precursor ion; (iii) the transitions for each peptide do not interfere with transitions from other peptides; and (iv) the transitions represent transitions due to breakage of peptide bond at different sites of the protein; and (d) selecting the peptides for each protein that best fit the criteria of step (b) and the transitions per peptide that best fit the criteria of step (c); thereby developing peptides and transitions for a LC-SRM-MS assay.

[0026] By plurality of proteins it is meant that at least 2, 3, 4, 5, 6, 7, 8, 9, 10, 15, 20, 25, 50, 100, 150, 200, 250, 300, 400, 450, 500 or more proteins. In certain embodiments, the plurality of proteins can encompass between 2 and 10, 10 and 20, 20 and 50, 50 and 100, 100 and 200 or 200 and 500 proteins. In other embodiments, the plurality of proteins can encompass between 250 and 450; or 300 and 400 proteins.

[0027] Trypsin-like proteases cleave peptide bonds following a positively charged amino acid (e.g., lysine (K) or arginine (R)). This specificity is driven by the residue which lies at the base of the enzyme's S1 pocket (generally a negatively charged aspartic acid or glutamic acid). Accordingly, in one embodiment of the method, the peptides do not include any peptide that is bounded by KK, KR, RK or RR, either upstream of downstream in the corresponding protein sequence. In another embodiment, each peptide of said set of peptides is unique to the corresponding protein.

[0028] Post-translational modification (PTM) is the chemical modification of a protein after its translation. It can include any modification following translation, including cleavage. It is one of the later steps in protein biosynthesis, and thus gene expression, for many proteins. It is desirable to avoid such peptides for the purpose of protein identification. Thus, in another embodiment, the peptides do not include peptides which were observed in post-translational modified forms.

[0029] In still another embodiment, each set of peptides is prioritized according to one or more of the following ordered set of criteria: (a) unique peptides first, then non-unique; (b) peptides with no observed post-translational modifications first, then those observed with post-translational modifications; (c) peptides within the mass range 800-3500 Da first, then those outside of 800-3500 Da; and (d) sorted by decreasing number of variant residues. In one embodiment, each set of peptides is prioritized according to all of the ordered set of criteria. In another embodiment, each prioritized set of peptides contains 1-5 peptides.

[0030] In certain embodiments, one or more liquid chromatography (LC) purification steps are performed prior to a subsequent LC-SRM-MS analysis step. Traditional LC analysis relies on the chemical interactions between sample components and column packing materials, where laminar flow of the sample through the column is the basis for separation of the analyte of interest from the test sample. The skilled artisan will understand that separation in such columns is a diffusional process. A variety of column packing materials are available for chromatographic separation of samples, and selection of an appropriate separation protocol is an empirical process that depends on the sample characteristics, the analyte of interest, the interfering substances present and their characteristics, etc. Various packing chemistries can be used depending on the needs (e.g., structure, polarity, and solubility of compounds being purified). In various embodiments the columns are polar, ion exchange (both cation and anion), hydrophobic interaction, phenyl, C-2, C-8, C-18 columns, polar coating on porous polymer, or others that are commercially available. During chromatography, the separation of materials is effected by variables such as choice of eluant (also known as a "mobile phase"), choice of gradient elution and the gradient conditions, temperature, etc. In certain embodiments, an analyte may be purified by applying a sample to a column under conditions where the analyte of interest is reversibly retained by the column packing material, while one or more other materials are not retained. In these embodiments, a first mobile phase condition can be employed where the analyte of interest is retained by the column, and a second mobile phase condition can subsequently be employed to remove retained material from the column, once the non-retained materials are washed through. Alternatively, an analyte may be purified by applying a sample to a column under mobile phase conditions where the analyte of interest elutes at a differential rate in comparison to one or more other materials. As discussed above, such procedures may enrich the amount of one or more analytes of interest relative to one or more other components of the sample.

[0031] The following parameters are used to specify an LC-SRM-MS assay of a protein under a particular LC-SRM-MS system: (1) a tryptic peptide of the protein; (2) the retention time (RT) of the peptide; (3) the m/z value of the peptide precursor ion; (4) the declustering potential used to ionize the precursor ion; (5) m/z value of a fragment ion generated from the peptide precursor ion; and (6) the collision energy (CE) used to fragment the peptide precursor ion that is optimized for the particular peptide.

[0032] In certain embodiments of the preceding methods, the two best peptides per protein and the two best transitions per peptide are selected based on experimental data resulting from LC-SRM-MS analysis of one or more of the following experimental samples: a biological disease sample, a biological control sample, and a mixture of synthetic peptides of interest. Biological samples include body fluids, tissue samples and cell samples. Body fluid samples can include blood, serum, sputum, genital secretions, cerebrospinal fluid, sweat or excreta such as urine. Body tissue samples can include lung, skin, brain, spine, bone, muscle, epithelial, liver, kidney, pancreas, gastrointestinal tract, cardiovascular tissue, heart or nervous tissue. Biological disease samples can include cancer, benign tumors, infected tissue and tissue subject to trauma. In a particular embodiment, the biological disease and biological control samples are processed using an immunodepletion method prior to LC-SRM-MS analysis. Immunodepletion involves removal of one or more proteins through the use of antibodies. Numerous immunodepletion techniques are known to those of skill in the art. In another embodiment, the biological disease and biological control samples are processed using an immunocapture method prior to LC-SRM-MS analysis. Immunocapture involves selection of one or more proteins through the use of antibodies. Numerous immunocapture techniques are known to those of skill in the art.

[0033] To facilitate accurate quantification of the peptide transitions by the methods disclosed herein, a set of isotopically-labeled synthetic versions of the peptides of interest may be added in known amounts to the sample for use as internal standards. Since the isotopically-labeled peptides have physical and chemical properties identical to the corresponding surrogate peptide, they co-elute from the chromatographic column and are easily identifiable on the resultant mass spectrum. The addition of the labeled standards may occur before or after proteolytic digestion. Methods of synthesizing isotopically-labeled peptides will be known to those of skill in the art. Thus, in another embodiment, the experimental samples contain internal standard peptides. Other embodiments may utilize external standards or other expedients for peptide quantification.

[0034] In yet another embodiment, the LC-SRM-MS analysis method specifies a maximum of 7000 transitions, including transitions of the internal standard peptides and transitions. As used herein, the term "transition" refers to the specific pair of m/z (mass-to-charge) values associated with the precursor and transition ions corresponding to a specific peptide and, therefore, to a specific protein.

[0035] In one embodiment of the method, the top two transitions per peptide are selected according to one or more of the following criteria (A): (1) the transitions exhibit the largest peak areas measured in either of the two biological experimental samples; (2) the transitions are not interfered with by other ions; (3) the transitions do not exhibit an elution profile that visually differs from those of other transitions of the same peptide; (4) the transitions are not beyond the detection limit of both of the two biological experimental samples; (5) the transitions do not exhibit interferences.

[0036] For the mass spectrometric analysis of a particular peptide, the quantities of the peptide transitions in the sample may be determined by integration of the relevant mass spectral peak areas, as known in the prior art. When isotopically-labeled internal standards are used, as described above, the quantities of the peptide transitions of interest are established via an empirically-derived or predicted relationship between peptide transition quantity (which may be expressed as concentration) and the area ratio of the peptide transition and internal standard peaks at specified transitions.

[0037] In another embodiment of the method, the top two peptides per protein are selected according to one or more of the following criteria (B): (1) one or more peptides exhibit two transitions according to criteria (A) and represent the largest combined peak areas of the two transitions according to criteria (A); and (2) one or more peptides exhibit one transition according to criteria (A) and represent the largest combined peak areas of the two transitions according to criteria (A).

Assays

[0038] The methods of the present disclosure allow the quantification of high abundance and low abundance plasma proteins that serve as detectable markers for various health states (including diseases and disorders), thus forming the basis for assays that can be used to determine the differences between normal levels of detectable markers and changes of such detectable markers that are indicative of changes in health status. In one aspect of the disclosure, provided herein is an assay developed according to the foregoing method, and embodiments thereof. In another aspect, provided herein is the use of an assay developed according to the foregoing method, and embodiments thereof, to detect a plurality of at least 200, 300 or more proteins in a single sample.

Definitions

[0039] As used herein, "transition" refers to a pair of m/z values associated with a peptide. Normally, labeled synthetic peptides are used as quality controls in SRM assays. However, for very large SRM assays, labeled peptides are not feasible. However, correlation techniques (Keary, Butler et al. 2008) were used to confirm the identity of protein transitions with high confidence. The correlation between a pair of transitions is obtained from their expression profile over all samples in the training set study detailed below. As expected, transitions from the same peptide are highly correlated. Similarly, transitions from different peptide fragments of the same protein are also highly correlated. In contrast, transitions form different proteins are not highly correlated. This methodology enables a statistical analysis of the quality of the protein's SRM assay. For example, if the correlation of the transitions from the two peptides from the same protein is above 0.5 then there is less than a 5% probability that the assay is false

[0040] As used herein, a "tryptic peptide" refers to the peptide that is formed by the treatment of a protein with trypsin.

[0041] As used herein, "RT" refers to "retention time", the elapsed time between injection and elution of an analyte.

[0042] As used herein, "m/z" indicates the mass-to-charge ratio of an ion.

[0043] As used herein "DP" refers to the "declustering potential", a voltage potential to dissolvate and dissociate ion clusters. It is also known as "fragmentor voltage" or "ion transfer capillary offset voltage" depending upon the manufacturer.

[0044] As used herein, "CE" refers to "collision energy", the amount of energy precursor ions receive as they are accelerated into the collision cell.

[0045] As used herein, "LC-SRM-MS" is an acronym for "selected reaction monitoring" and may be used interchangeably with "LC-MRM-MS".

[0046] As used herein, "MS/MS" represents tandem mass spectrometry, which is a type of mass spectrometry involving multiple stages of mass analysis with some form of fragmentation occurring in between the stages.

[0047] As used herein, "ISP" refers to "internal standard peptides".

[0048] As used herein, "HGS" refers to "human gold standard", which is comprised of a pool of plasma from healthy individuals.

[0049] As used herein, "MGF" refers to "Mascot generic file". Mascot is a search engine that uses mass spectrometry data to identify proteins from primary sequence databases. A Mascot generic file is a plain text (ASCII) file containing peak list information and, optionally, search parameters.

[0050] Mascot is a tool for assessing mass spectrometry data against protein sequences. This data can be acquired from any mass spectrometry technique including MALDI-TOF and electrospray ionization MS (including LC-SRM-MS) data. Mascot uses a `probability-based MOWSE` algorithm to estimate the significance of a match (i.e., that the observed transitions correspond to a particular protein). The total score is the absolute probability that the observed match is a random event. They are reported as -10.times.LOG10(P), where P is the absolute probability. Lower probabilities, therefore, are reported as higher scores. For example, if the absolute probability that an observed match is random is 1.times.10.sup.-12, Mascot reports it as 120.

[0051] The disclosure also provides compositions. These compositions can include any of the transition ions described in Table II. These transition ions exist while peptides derived from the proteins in Table II are undergoing analysis with LC-SRM-MS. In one embodiment, the composition includes any of the transition ions described in Table II. In another embodiment, the composition includes any two transition ions described in Table II. In other embodiments, the composition includes, any 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 100, 110, 120, 130, 140, 150, 160, 170, 180, 190, 200, 210, 220, 230, 240, 250, 260, 270, 280, 290, 300 or 331 transition ions described in Table II.

[0052] In another embodiment, each of the transition ions in the composition corresponds and/or is derived from a different protein. In another embodiment, 90% of the transition ions in the composition correspond with and/or are derived from a protein that no other transition ion in the composition corresponds. In other embodiments, 80, 70, 60, 50, 40, 30, 20, 10 or 0% of the transition ions in the composition correspond and/or are derived from a protein that no other transition ion in the composition corresponds.

[0053] The compositions described herein included synthetic peptides. Synthetic peptides can be used as controls for the abundance of proteins they are derived from and/or correspond. In certain embodiments, the abundance of the synthetic peptides is defined and the results are compared to LC-SRM-MS results from a peptide found in a sample to the LC-SRM-MS results in the corresponding synthetic peptide. This allows for the calculation of the abundance of the peptide in the sample. In certain embodiments, by knowing the abundance of a peptide in a sample, the abundance of the protein it corresponded to is determined.

[0054] Synthetic peptides can be generated using any method known in the art. These methods can include recombinant expression techniques such as expression in bacteria or in vitro expression in eukaryotic cell lysate. These methods can also include solid phase synthesis.

[0055] In one embodiment, the composition includes synthetic peptides selected from any of the peptides described in Table II. In another embodiment, the composition included any two peptides described in Table II. In other embodiments, the composition included, any 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 100, 110, 120, 130, 140, 150, 160, 170, 180, 190, 200, 210, 220, 230, 240, 250, 260, 270, 280, 290, 300, 331 or more peptides described in Table II.

[0056] In another embodiment, each of the peptides in the composition each corresponds with a different protein. In another embodiment, 90% of the peptides in the composition correspond with a protein that no other peptide in the composition corresponds with. In other embodiments, 80, 70, 60, 50, 40, 30, 20, 10 or 0% of the peptides in the composition correspond with from a protein that no other peptide in the composition corresponds with.

[0057] The peptides can be isotopically labeled. The isotopes with which they can be labeled include .sup.13C, .sup.2H, .sup.15N and .sup.18O. The peptides can also include a polar solvent. Polar solvents can include water and mixtures of ethanol and water.

[0058] In certain embodiments, the samples described herein are taken from mammals. These mammals include rats, mice, rabbits, dogs, non-human primates and humans. Samples can be isolated from any tissue or organ or from any bodily fluid. Organs from which samples can be taken include skin, heart, lung, brain, kidney, liver, pancreas, spleen, testes, ovaries, gall bladder, thymus, thyroid, eye, ear, nose, mouth, tongue, penis, vagina, bladder or larynx. Tissues include nervous tissue, vascular tissue, muscle, bone, gastrointestinal tract, epithelial tissue, fibroblastic tissue, mucous membranes, hair, skin, reproductive tissue and connective tissue. Body fluids and excretions include, blood, serum, saliva, urine, semen, vaginal secretions, excrement, bile, tears, lymph, ear wax, mucous, shed skin, finger nails, toe nails, skin oils, sweat and dandruff.

[0059] The relative abundance of one or more of the proteins represented by the transition ions and synthetic peptides described above can be used to diagnose, determine likelihood of the presence of, develop prognoses for and/or stage various diseases and pathologies. Often the organ, tissue or bodily fluid or excretion from which the sample is taken is distinct from the organ, tissue or bodily fluid or excretion involved with the disease or pathology. For example, the presence of Alzheimer's disease can be determined from a sample taken from blood. Any type of body fluid may be used in the assays.

[0060] Diseases and pathologies that status, diagnosis, presence or prognosis can be found using the transition ions and/or synthetic peptides described herein include cancer, metabolic diseases, neurological disorders, infectious diseases and cardiovascular disorders.

EXAMPLES

I. Exemplary Standard Operating Procedure

Protein Selection

[0061] Proteins known to be over-expressed or under-expressed in Alzheimer's disease patients were obtained (through literature searching, experimental data or proprietary databases) as shown in Table I. The set of proteins was reduced to a set of 357 proteins (see Table II) by prioritizing those proteins that have been previously detected my LC-MS/MS in blood (serum or plasma).

[0062] Selected proteins were then identified by their UniProt protein name and accession, their Entrez gene symbol and gene name, the isoform accession and their amino acid sequence. The canonical isoform in UniProt was selected if a protein has more than one isoform.

Peptide Selection for Synthesis

[0063] The five best peptides per protein for LC-SRM-MS assay were selected for as follows. Fully tryptic peptides having a monoisotopic mass of 800-3500 mass units, without miscleavages, not containing a cysteine (C) or a methionine (M), without having high miscleavage probability were selected. Further, any peptide that was bounded by KK, KR, RK or RR (either upstream or downstream) in the corresponding protein sequence was not selected.

[0064] Peptides were selected that were unique to the protein of interest. Peptides were only selected that match only one protein or protein family including analogues of the one protein, when searched in protein databases. Further, peptides which were observed in post-translational modified forms were not selected. Databases were assessed that showed expression of the proteins from which the peptides were isolated in human blood. Also databases of good quality MS peptides were searched. Peptides that appeared in human blood and were good quality MS peptides were favored. If these methods did not result in a sufficient number of peptides, rules were relaxed in a step wise manner to allow a greater number of peptides until a sufficient number was reached. The purity of the synthesized peptides was >75% and the amount of material was .gtoreq.25 .mu.g. Peptides did not need to be desalted.

[0065] The four best transitions per peptide are then selected and optimized based on experimental results from a mixture of synthetic peptides. LC-SRM-MS-triggered MS/MS spectra was acquired for each synthetic peptide, using a QTRAP 5500 instrument. One spectrum or the doubly--and one for the triply--charged precursor ion was collected for each of the identified peptides (Mascot score .gtoreq.15), retention time was recorded for the four most intense b or y transition ions. The selected transition ions possessed m/z values were at least 30 m/z above or below those of the precursor ions; they did not interfere with other synthetic peptides; and they were transition ions due to breakage of peptide bond at different sites.

[0066] If an insufficient percentage of the synthetic peptides were acquired, the steps were repeated. In some cases, the second transition with first with theoretical y+ ions with m/z values at least 30 m/z above those of the doubly charged precursor ion was selected if an insufficient percentage was acquired. Peptides that failed to trigger the acquisition of MS/MS spectrum were discarded.

II. Exemplary Protein list

[0067] The abundance of the following proteins can be assessed substantially simultaneously using the MS-LC-SRM-MS system described herein. Transitions from these proteins can be used to diagnose diseases including Alzheimer's disease when their abundance is measured in a biological specimen from a subject to be diagnosed for Alzheimer's disease. In one embodiment, the abundances of these proteins are measure in the blood serum of the subject.

III. Exemplary LC-SRM-MS-Triggered MS/MS Results (Table I)

TABLE-US-00001 [0068] Re- Declus- ten- tering Col- tion Poten- lision Protein Peptide Transition Q1 Q3 Time tial Energy ITIH1_HUMAN GSLVQASEANL GSLVQASEANLQAAQDFVR 1002.5 1232.6 23.1 130.3 49.6 QAAQDFVR _1002.5_1232.6 PEDF_HUMAN TSLEDFYLDEE TSLEDFYLDEER_758.8_ 758.8 1215.4 20.3 98.9 38.9 R 1215.4 PON1_HUMAN LIALTLLGMGL LIALTLLGMGLALFR_801.5_ 801.5 1191.6 23.7 104.4 37.8 ALFR 1191.6 GELS_HUMAN EVQGFESATFL EVQGFESATFLGYFK_861.9 861.9 1162.5 23.3 112.2 40.4 GYFK _1162.5 HEMO_HUMAN LYLVQGTQVYV LYLVQGTQVYVFLTK_886.5 886.5 1155.7 23.2 115.4 41.5 FLTK _1155.7 PEDF_HUMAN LQSLFDSPDFS LQSLFDSPDFSK_692.3_ 692.3 1142.5 20.4 90.3 33 K 1142.5 ARSA_HUMAN QSLFFYPSYPD QSLFFYPSYPDEVR_874.4_ 874.4 1125.6 23.1 113.8 44 EVR 1125.6 VTNC_HUMAN IYISGMAPRPS IYISGMAPRPSLAK_501.9_ 501.9 1114.5 22.5 65.8 26.1 LAK 1114.5 PEDF_HUMAN TSLEDFYLDEE TSLEDFYLDEER_758.8_ 758.8 1086.4 20.3 98.9 38.9 R 1086.4 F13A_HUMAN LIASMSSDSLR LIASMSSDSLR_590.3_1066.3 590.3 1066.3 14 77.1 28.5 MTOR_HUMAN LIHQLLTDIGR LIHQLLTDIGR_639.9_1052.6 639.9 1052.6 19.8 83.5 36.7 CBPN_HUMAN EALIQFLEQVH EALIQFLEQVHQGIK_585_ 585 1051.7 23.1 76.5 30.4 QGIK 1051.7 CLUS_HUMAN ELDESLQVAER ELDESLQVAER_644.8_ 644.8 1046.4 13.4 84.2 36.9 1046.4 APOA4_HUMAN ALVQQMEQLR ALVQQMEQLR_608.3_ 608.3 1031.6 17.4 79.5 29.3 1031.6 ITIH2_HUMAN FYNQVSTPLLR FYNQVSTPLLR_669.4_ 669.4 1027.6 18.3 87.3 35 1027.6 NMDE1_HUMAN FTYDLYLVTNG FTYDLYLVTNGK_717.4_ 717.4 1022.5 22 93.5 37.1 K 1022.5 KCC1G_HUMAN DIYPSVSLQIQ DIYPSVSLQIQK_695.88_ 695.88 999.6 17.14 90.8 33.1 K 999.6 P4K2A_HUMAN AYPFYWAWLPQ AYPFYWAWLPQAK_820.92 820.92 999.5 25.1 106.9 38.6 AK _999.5 TRFR_HUMAN ESDHFSTELDD ESDHFSTELDDITVTDTYLS 863.4 999.5 20.37 112.4 38.6 ITVTDTYLSAT ATK_863.4_999.5 K A1BG_HUMAN TPGAAANLELI TPGAAANLELIFVGPQHAG 766.07 999.5 21.76 99.8 42.6 FVGPQHAGNYR NYR_766.07_999.5 HCN2_HUMAN AEALPPEAADE AEALPPEAADEGGPR_ 740.36 998.5 10.48 96.5 41.1 GGPR 740.36_998.5 CSF1R_HUMAN VIPGPPALTLVP VIPGPPALTLVPAELVR_ 871.5 997.6 23.3 113.4 46.8 AELVR 871.5_997.6 TNF6B_HUMAN LLQALEAPEGWG LLQALEAPEGWGPTPR_ 867.96 996.5 19.64 113 40.7 PTPR 867.96_996.5 A1BG_HUMAN LETPDFQLFK LETPDFQLFK_619.3_995.6 619.3 995.6 23.1 80.9 29.7 TADBP_HUMAN FGGNPGGFGNQ FGGNPGGFGNQGGFGNSR_ 863.89 993.5 14.09 112.4 46.5 GGFGNSR 863.89_993.5 PVRL1_HUMAN VEFLRPSFTDG VEFLRPSFTDGTIR_546.63_ 546.63 993.5 18.3 71.5 31.4 TIR 993.5 LCN8_HUMAN IVGSEIDSTGK IVGSEIDSTGK_553.29_992.5 553.29 992.5 9.21 72.4 26.8 SLIK1_HUMAN DIDPGAFQDLN DIDPGAFQDLNK_666.83_ 666.83 989.5 15.66 87 31.8 K 989.5 NDF6_HUMAN NYIWALSEILR NYIWALSEILR_689.38_987.6 689.38 987.6 25.16 89.9 32.8 ANGT_HUMAN ADSQAQLLLST ADSQAQLLLSTVVGVFTAP 822.46 983.6 25.25 107.1 39.5 VVGVFTAPGLH GLHLK_822.46_983.6 LK SCN2A_HUMAN FDPDATQFIEF FDPDATQFIEFAK_764.87_ 764.87 983.5 20.36 99.7 36.2 AK 983.5 AT12A_HUMAN TPEIYSVELSG TPEIYSVELSGTK_712.37_ 712.37 983.5 15.53 92.9 36.8 TK 983.5 DIRA2_HUMAN ESYIPTVEDTY ESYIPTVEDTYR_736.85_ 736.85 980.5 15.41 96.1 31.9 R 980.5 FINC_HUMAN SYTITGLQPGT SYTITGLQPGTDYK_772.39_ 772.39 978.5 15.02 100.6 36.5 DYK 978.5 PON1_HUMAN LIALTLLGMGL LIALTLLGMGLALFR_801.5_ 801.5 977.5 23.7 104.4 37.8 ALFR 977.5 MMP17_HUMAN VLDGELEVAPG VLDGELEVAPGYPQSTAR_ 951.48 976.5 15.62 123.7 44.4 YPQSTAR 951.48_976.5 MAP4_HUMAN VALSSETEVAL VALSSETEVALAR_673.37_ 673.37 975.5 14.63 87.9 41.1 AR 975.5 AGAP2_HUMAN YEQLLFLAPL YEQLLFLAPLSTSEEPLGR_ 721.72 975.5 24.92 94.1 37.4 STSEEPLGR 721.72_975.5 CHAD_HUMAN NNFPVLAANS NNFPVLAANSFR_675.35_ 675.35 974.5 18.58 88.1 32.2 FR 974.5 LCK_HUMAN AQSLTTGQEG AQSLTTGQEGFIPFNFVAK_ 685.69 973.5 23.14 89.5 32.5 FIPFNFVAK 685.69_973.5 PSMG1_HUMAN TSESTGSLPS TSESTGSLPSPFLR_739.88_ 739.88 973.5 17.54 96.4 44.1 PFLR 973.5 ITIH4_HUMAN LPEGSVSLII LPEGSVSLIILLTDGDPTVGE 865.13 970.5 25.08 112.6 38.7 LLTDGDPTVG TNPR_865.13_970.5 ETNPR ATS1_HUMAN GAFYLLGEAY GAFYLLGEAYFIQPLPAASE 1157.1 968.5 25.09 150.3 50.4 FIQPLPAASE R_1157.1_968.5 R CADH7_HUMAN VVYSILQGQP VVYSILQGQPYFSVEPK_ 652.02 966.5 21.48 85.1 30.8 YFSVEPK 652.02_966.5 GRIA2_HUMAN GYHYIIANLG GYHYIIANLGFTDGDLLK_ 670.68 965.5 23.39 87.5 28.8 FTDGDLLK 670.68_965.5 CAD22_HUMAN LLEPESEFII LLEPESEFIIK_659.37_962.5 659.37 962.5 19.62 86.1 31.5 K ARSA_HUMAN QSLFFYPSYP QSLFFYPSYPDEVR_874.42_ 874.42 962.5 21.26 113.8 41 DEVR 962.5 ANO3_HUMAN AEQVNTEENK AEQVNTEENK_581.27_961.5 581.27 961.5 6.06 76 28.1 GAS6_HUMAN IAVAGDLFQP IAVAGDLFQPER_658.35_ 658.35 961.5 17.91 85.9 31.5 ER 961.5 PTPRT_HUMAN AVGSLDPSAD AVGSLDPSADLSSQR_ 751.88 960.5 13.34 98 35.6 LSSQR 751.88_960.5 PAQR6_HUMAN FLELESPGLS FLELESPGLSK_610.33_959.5 610.33 959.5 17.4 79.7 29.4 K HEMO_HUMAN NFPSPVDAAF NFPSPVDAAFR_610.81_ 610.81 959.5 17.76 79.8 29.4 R 959.5 CB085_HUMAN GSLALPFPAD GSLALPFPADVQGK_700.38 700.38 958.5 19.32 91.3 33.3 VQGK _958.5 CO4A_HUMAN VTASDPLDTL VTASDPLDTLGSEGALSPG 828.44 956.6 24.54 107.9 39.8 GSEGALSPGG GVASLLR_828.44_956.6 VASLLR HS3S5_HUMAN LITEPLPELQ LITEPLPELQLVEK_811.47_ 811.47 955.5 21.7 105.7 41.2 LVEK 955.5 F13A_HUMAN LIASMSSDSL LIASMSSDSLR_590.3_953.2 590.3 953.2 14 77.1 28.5 R MMP9_HUMAN SLGPALLLLQ SLGPALLLLQK_576.87_ 576.87 952.6 22.28 75.4 27.9 K 952.6 ITAM_HUMAN LFTALFPFEK LFTALFPFEK_606.84_952.5 606.84 952.5 24.87 79.3 29.2 KCC2A_HUMAN FYFENLWSR FYFENLWSR_631.3_951.5 631.3 951.5 23.34 82.4 30.3 AGAL_HUMAN NFADIDDSWK NFADIDDSWK_605.77_949.4 605.77 949.4 17.18 79.1 29.2 CAD19_HUMAN AVEPESEFVI AVEPESEFVIK_624.33_948.5 624.33 948.5 15.88 81.5 30 K SEPP1_HUMAN LVYHLGLPFS LVYHLGLPFSFLTFPYVEEA 861.8 948.5 25.31 112.2 41.5 AIFLTFPYVE IK_861.8_948.5 EK PCDA5_HUMAN LNASDADEGI LNASDADEGINK_623.8_ 623.8 948.4 9.07 81.5 29.9 NK 948.4 ADA12_HUMAN FGFGGSTDSG FGFGGSTDSGPIR_649.31_ 649.31 946.5 15.66 84.8 34.1 PIR 946.5 CHAD_HUMAN YLETLWLDNT YLETLWLDNTNLEK_876.45 876.45 946.5 21.97 114.1 41.1 NLEK _946.5 PDIA2_HUMAN ADFPVDEELG ADFPVDEELGLDLGDLSR_ 980.98 945.5 23.42 127.5 45.7 LDLGDLSR 980.98_945.5 41_HUMAN NFMESVPEPR NFMESVPEPR_603.3_944.4 603.3 944.4 16 78.8 32 PON1_HUMAN IQNILTEEPK IQNILTEEPK_592.83_943.5 592.83 943.5 14.3 77.5 28.6 KLK3_HUMAN LSEPAELTDA LSEPAELTDAVK_636.84_ 636.84 943.5 14.48 83.2 30.5 VK 943.5 LAMB2_HUMAN SLADVDAILA SLADVDAILAR_572.32_ 572.32 943.5 19.32 74.8 27.7 R 943.5 NFL_HUMAN VLEAELLVLR VLEAELLVLR_577.86_942.6 577.86 942.6 21.86 75.5 27.9 IPSP_HUMAN AAAATGTIFT AAAATGTIFTFR_613.83_ 613.83 942.5 18.99 80.2 29.5 FR 942.5 PEDF_HUMAN LQSLFDSPDF LQSLFDSPDFSK_692.3_ 692.3 942.4 20.4 90.3 33 SK 942.4 F123A_HUMAN APAAPDPASV APAAPDPASVDPPSDPSAD 966.95 941.4 11.35 125.7 51 RDPPSDPSAD R_966.95_941.4 GALR3_HUMAN FLWAAVGPAG FLWAAVGPAGAAAAEAR_ 814.9 941.2 20.2 106.1 41.4 AAAAEAR 814.9_941.2 SIG12_HUMAN NIPVATNNPA NIPVATNNPAR_583.82_ 583.82 939.5 9.32 76.3 28.2 R 939.5 FCGRN_HUMAN SGLPAPWISL SGLPAPWISLR_598.84_939.5 598.84 939.5 21.69 78.3 28.8 R CBPN_HUMAN EALIQFLEQV EALIQFLEQVHQGIK_585_ 585 938.6 23.1 76.5 30.4

HQGIK 938.6 LAT2_HUMAN DIFGGLAGFL DIFGGLAGFLR_583.32_ 583.32 937.5 25.16 76.2 28.2 R 937.5 MMP9_HUMAN AFALWSAVTP AFALWSAVTPLTFTR_ 840.96 934.5 25.11 109.5 36.5 LTFTR 840.96_934.5 APOL4_HUMAN NLTPYVAIED NLTPYVAIEDK_631.84_ 631.84 934.5 15.55 82.5 27.3 K 934.5 TRFR_HUMAN ESDHFSTELD ESDHFSTELDDITVTDTYLS 863.4 933.4 20.37 112.4 47.6 DITVTDTYLS ATK_863.4_933.4 ATK APOA4_HUMAN ALVQQMEQLR ALVQQMEQLR_608.3_932.5 608.3 932.5 17.4 79.5 29.3 HEMO_HUMAN SGAQATWTEL SGAQATWTELPWPHEK_ 613.3 932.4 19.54 80.1 25.8 PWPHEK 613.3_932.4 MAP4_HUMAN TDYIPLLDVD TDYIPLLDVDEK_710.86_ 710.86 928.5 20.87 92.7 33.8 EK 928.5 F13A_HUMAN STVLTIPEII STVLTIPEIIIK_663.9_926.6 663.9 926.6 23.2 86.6 31.7 IK LIPE_HUMAN DDFSLGNVHL DDFSLGNVHLIGYSLGAHV 974.49 926.5 24.54 126.7 47.2 IGYSLGAHVA AGYAGNFVK_974.49_926.5 GYAGNFVK PKDRE_HUMAN GAQAAVSGAP GAQAAVSGAPGGLLR_ 662.87 926.5 14.12 86.5 40.7 GGLLR 662.87_926.5 GALR3_HUMAN LLAGGGQGPE LLAGGGQGPEPR_576.31_ 576.31 925.4 9.45 75.3 27.9 PR 925.4 ITIH2_HUMAN SSALDMENFR SSALDMENFR_585.3_924.3 585.3 924.3 16.3 76.5 34.3 CLUS_HUMAN ASSIIDELFQ ASSIIDELFQDR_697.35_ 697.35 922.4 23.8 91 33.2 DR 922.4 TYRO_HUMAN LTGDENFTIP LTGDENFTIPYWDWR_ 956.95 922.4 23.81 124.4 44.6 YWDWR 956.95_922.4 NTRK2_HUMAN ITNISSDDSG ITNISSDDSGK_568.8_922.2 568.8 922.2 5.6 74.4 27.5 K WNK4_HUMAN VTSVSDQNDR VTSVSDQNDR_560.77_920.4 560.77 920.4 6.17 73.3 27.2 CAR14_HUMAN TASDQESGDE TASDQESGDEELNR_775.83 775.83 919.4 7.6 101.1 36.6 ELNR _919.4 ODP2_HUMAN LSEGDLLAEI LSEGDLLAEIETDK_766.89_ 766.89 918.5 20.65 99.9 36.2 ETDK 918.5 RGS20_HUMAN LFGLLSSPLS LFGLLSSPLSSLAR_730.93_ 730.93 917.5 25.08 95.3 34.7 SLAR 917.5 AGAP2_HUMAN SLDLDDWPR SLDLDDWPR_558.77_916.4 558.77 916.4 18.55 73.1 27.1 RELN_HUMAN IISVELPGDA IISVELPGDAK_571.33_915.5 571.33 915.5 16.13 74.7 27.6 K LTBP1_HUMAN TSTDLDVDVD TSTDLDVDVDQPK_716.84_ 716.84 915.4 12.25 93.5 34 QPK 915.4 SYTL4_HUMAN YEIPESLLAQ YEIPESLLAQR_659.85_913.5 659.85 913.5 17.79 86.1 31.5 R C1QT4_HUMAN SLVGSDAGPG SLVGSDAGPGPR_556.79_ 556.79 912.5 9.81 72.8 30 PR 912.5 IC1_HUMAN FQPTLLTLPR FQPTLLTLPR_593.35_910.6 593.35 910.6 20.26 77.5 28.6 CK041_HUMAN GTSSSPLAVA GTSSSPLAVASGPAK_665.35 665.35 910.5 11.51 86.8 31.8 SGPAK _910.5 LAMB2_HUMAN AMDYDLLLR AMDYDLLLR_555.3_907.5 555.3 907.5 22 72.6 29.9 EMIL2_HUMAN LIVPEPDVDF LIVPEPDVDFDAK_729.38_ 729.38 906.4 18.52 95.1 40.6 DAK 906.4 GRN_HUMAN EVVSAQPATF EVVSAQPATFLAR_694.88_ 694.88 903.5 16.12 90.6 33.1 LAR 903.5 ARSA_HUMAN DPGENYNLLG DPGENYNLLGGVAGATPEV 809.42 903.4 24.24 105.4 44.8 GVAGATPEVL LQALK_809.42_903.4 QALK ITIH3_HUMAN DYIFGNYIER DYIFGNYIER_645.31_898.4 645.31 898.4 19.24 84.2 30.9 ARSA_HUMAN DPGENYNLLG DPGENYNLLGGVAGATPEV 809.42 897.5 24.24 105.4 38.8 GVAGATPEVL LQALK_809.42_897.5 QALK NCAN_HUMAN VEAHGEATAT VEAHGEATATAPPSPAAET 645.65 897.5 8.33 84.3 30.5 APPSPAAETK K_645.65_897.5 ITIH4_HUMAN LPEGSVSLII LPEGSVSLIILLTDGDPTVGE 865.13 896.5 25.08 112.6 41.7 LLTDGDPTVG TNPR_865.13_896.5 ETNPR NEUM_HUMAN EEEPEADQEH EEEPEADQEHA_642.26_ 642.26 896.4 6.66 83.9 30.8 A 896.4 TADBP_HUMAN FTEYETQVK FTEYETQVK_572.78_896.4 572.78 896.4 10.48 74.9 27.7 DCBD1_HUMAN HEYALPLAPP HEYALPLAPPEPEYATPIVE 798.08 895.5 19.34 104 38.3 REPEYATPIV R_798.08_895.5 E A1BG_HUMAN LETPDFQLFK LETPDFQLFK_619.33_894.5 619.33 894.5 21.25 80.9 29.8 MMP16_HUMAN ILLTFSTGR ILLTFSTGR_504.3_894.4 504.3 894.4 18.9 66.1 27.7 HPCA_HUMAN LSLEEFIR LSLEEFIR_503.78_893.5 503.78 893.5 21.7 66 27.7 LCN8_HUMAN IVGSEIDSTG IVGSEIDSTGK_553.29_893.4 553.29 893.4 9.21 72.4 26.8 K ANO3_HUMAN IAYLLTNLEY IAYLLTNLEYPR_733.41_ 733.41 892.5 21.46 95.6 34.8 PR 892.5 VGFR3_HUMAN SGVDLADSNQ SGVDLADSNQK_567.28_ 567.28 890.4 8.61 74.2 27.5 K 890.4 TERA_HUMAN GILLYGPPGT GILLYGPPGTGK_586.84_ 586.84 889.5 16.52 76.7 28.3 GK 889.5 HCN1_HUMAN GVPPAPPPPA GVPPAPPPPAAALPR_704.41 704.41 889.5 13.39 91.9 42.5 AALPR _889.5 AACT_HUMAN ITLLSALVET ITLLSALVETR_608.4_888.4 608.4 888.4 23.3 79.5 32.3 R SEPP1_HUMAN LPTDSELAPR LPTDSELAPR_549.79_888.4 549.79 888.4 11.86 71.9 29.7 CSF1_HUMAN ISSLRPQGLS ISSLRPQGLSNPSTLSAQPQL 813.11 886.5 15.09 105.9 48 NPSTLSAQPQ SR_813.11_886.5 LSR S12A5_HUMAN DGIVPFLQVF DGIVPFLQVFGHGK_505.28_ 505.28 885.5 24.51 66.2 26.3 GHGK 885.5 SEMG1_HUMAN DVSQSSIYSQ DVSQSSIYSQTEEK_800.87_ 800.87 884.4 10.52 104.3 37.7 TEEK 884.4 GALR3_HUMAN FLWAAVGPAG FLWAAVGPAGAAAAEAR_ 814.9 884.2 20.2 106.1 41.4 AAAAEAR 814.9_884.2 AMPB_HUMAN ETFASTASQL ETFASTASQLHSNVVNYVQ 911.14 882.5 24.72 118.5 41 HSNVVNYVQQ QIVAPK_911.14_882.5 IVAPK AGAL_HUMAN SILDWTSFNQ SILDWTSFNQER_748.36_ 748.36 881.4 21.18 97.5 35.4 ER 881.4 CSF1R_HUMAN ALTFELTLR ALTFELTLR_532.3_879.4 532.3 879.4 23.2 69.7 28.9 OLIG1_HUMAN FPHLVPASLG FPHLVPASLGLAAVQAQFS 694.39 878.5 24.11 90.6 30 LAAVQAQFSK K_694.39_878.5 SPB5_HUMAN DVEDESTGLE DVEDESTGLEK_611.28_ 611.28 878.4 9.19 79.9 29.4 K 878.4 PCDAA_HUMAN LNASDSDEGI LNASDSDEGINK_631.8_ 631.8 877.4 7.92 82.5 30.3 NK 877.4 GP2_HUMAN NTLSLVNDFI NTLSLVNDFIIR_702.9_876.5 702.9 876.5 24.08 91.7 33.4 IR NMDE3_HUMAN SVEDALTSLK SVEDALTSLK_531.79_876.5 531.79 876.5 16.02 69.6 25.9 MTOR_HUMAN VLGLLGALDP VLGLLGALDPYK_629.87_ 629.87 876.5 24.65 82.3 30.2 YK 876.5 NRG3_HUMAN EYVPTDLVDS EYVPTDLVDSK_633.32_ 633.32 874.5 14.42 82.7 30.4 K 874.5 NMDE1_HUMAN GVEDALVSLK GVEDALVSLK_515.79_874.5 515.79 874.5 16.75 67.5 25.2 EPHA8_HUMAN WTAPEAIAFR WTAPEATAFR_581.31_874.5 581.31 874.5 18.23 76 25.1 P4K2A_HUMAN IYQGSSGSYF IYQGSSGSYFVK_668.33_ 668.33 874.4 13.39 87.2 31.9 VK 874.4 CLC4M_HUMAN AAVGELPDQS AAVGELPDQSK_557.79_ 557.79 873.4 9.74 73 27 K 873.4 HRG_HUMAN ADLFYDVEAL ADLFYDVEALDLESPK_ 912.95 872.5 23.8 118.8 45.7 DLESPK 912.95_872.5 SHSA7_HUMAN LVSQEHLLLS LVSQEHLLLSSPEALR_598_ 598 872.5 17.69 78.1 37 SPEALR 872.5 LAMB2_HUMAN SLADVDAILA SLADVDAILAR_572.3_872.4 572.3 872.4 20.9 74.8 27.7 R CAD11_HUMAN VLDVNDNAPK VLDVNDNAPK_542.79_ 542.79 872.4 9.67 71 26.4 872.4 PAR6B_HUMAN AVSTANPLLR AVSTANPLLR_521.31_871.5 521.31 871.5 13.55 68.2 25.4 S39A4_HUMAN AGLWASHADH AGLWASHADHLLALLESPK 677.03 870.5 24.55 88.3 35.1 LLALLESPK _677.03_870.5 CERU_HUMAN GAYPLSIEPI GAYPLSIEPIGVR_686.39_ 686.39 870.5 19.06 89.5 41.7 GVR 870.5 ARHG7_HUMAN SYYNVVLQNI SYYNVVLQNILETENEYSK_ 1153.56 870.4 24.95 149.8 50.3 LETENEYSK 1153.56_870.4 PTPRO_HUMAN LIGLDIPHFA LIGLDIPHFAADLPLNR_ 625.69 869.5 24.19 81.7 35.5 ADLPLNR 625.69_869.5 CO4A_HUMAN VTASDPLDTL VTASDPLDTLGSEGALSPG 828.44 869.5 24.54 107.9 39.8 GSEGALSPGG GVASLLR_828.44_869.5 VASLLR CF072_HUMAN VFFPVSEYK VFFPVSEYK_558.29_869.4 558.29 869.4 17.92 73 27.1 FEZ1_HUMAN VPTLLTDYIL VPTLLTDYILK_638.38_865.5 638.38 865.5 23.17 83.4 33.6 K LRTM2_HUMAN LDLSNNFLDR LDLSNNFLDR_603.81_865.4 603.81 865.4 18.13 78.9 29.1 CATD_HUMAN LSPEDYTLK LSPEDYTLK_533.28_865.4 533.28 865.4 13.66 69.8 26 APOC2_HUMAN TAAQNLYEK TAAQNLYEK_519.27_865.4 519.27 865.4 8.89 68 25.3 CO2_HUMAN HAFILQDTK HAFILQDTK_536.79_864.5 536.79 864.5 12.44 70.2 29.1 SPB5_HUMAN DVPFGFQTVT DVPFGFQTVTSDVNK_ 827.41 863.4 18.74 107.7 41.9 SDVNK 827.41_863.4

GOLM1_HUMAN DLSENNDQR DLSENNDQR_545.74_862.4 545.74 862.4 6.25 71.4 26.5 APOA4_HUMAN ISASAEELR ISASAEELR_488.26_862.4 488.26 862.4 10.18 64 24 NTRK2_HUMAN SNEIPSTDVT SNEIPSTDVTDK_653.31_ 653.31 862.4 10.04 85.3 31.2 DK 862.4 ITIH1_HUMAN EVAFDLEIPK EVAFDLEIPK_580.8_861.5 580.8 861.5 21.7 75.9 28.1 PGCB_HUMAN ELEAPSEDNS ELEAPSEDNSGR_652.29_ 652.29 861.4 8.33 85.1 31.2 861.4 A2MG_HUMAN LLIYAVLPTG LLIYAVLPTGDVIGDSAK_ 615.68 861.4 23.29 80.4 28.9 DVIGDSAKGR 615.68_861.4 PCDA5_HUMAN LNASDADEGI LNASDADEGINK_623.8_ 623.8 861.4 9.07 81.5 29.9 NK 861.4 TRPV5_HUMAN ASLALPTSSL ASLALPTSSLSR_601.84_ 601.84 860.5 15.54 78.6 38 SR 860.5 NETR_HUMAN VSAFVPWIK VSAFVPWIK_523.81_860.5 523.81 860.5 21.34 68.6 25.5 CERU_HUMAN GPEEEHLGIL GPEEEHLGILGPVIWAEVG 829.77 860.4 25.08 108 39.9 GPVIWAEVGD DTIR_829.77_860.4 TIR TM9S1_HUMAN SLSLGEVLDG SLSLGEVLDGDR_630.83_ 630.83 860.4 19.62 82.4 30.3 DR 860.4 GP125_HUMAN WDFDLLNGQG WDFDLLNGQGGWK_768.37 768.37 859.4 23.77 100.1 36.3 GWK _859.4 APC2_HUMAN LAAQEGPLSL LAAQEGPLSLSR_621.35_ 621.35 858.5 14.4 81.2 32.8 SR 858.5 OTOAN_HUMAN GSSGSFLQPD GSSGSFLQPDITER_747.37_ 747.37 858.4 15.66 97.4 35.4 ITER 858.4 ARSE_HUMAN LAGGEVPQDR LAGGEVPQDR_521.27_857.4 521.27 857.4 7.99 68.2 25.4 EDNRB_HUMAN LTLYNQNDPN LTLYNQNDPNR_674.34_ 674.34 857.4 11.47 88 35.2 R 857.4 PTN5_HUMAN VLQAEELHEK VLQAEELHEK_598.32_855.4 598.32 855.4 9.59 78.2 31.8 AFAM_HUMAN HELTDEELQS HELTDEELQSLFTNFANVV 817.07 854.4 25.08 106.4 42.2 LFTNFANVVD DK_817.07_854.4 K KALRN_HUMAN DFFLAELEK DFFLAELEK_556.29_849.5 556.29 849.5 22.36 72.8 27 LIPE_HUMAN DDFSLGNVHL DDFSLGNVHLIGYSLGAHV 974.49 848.4 24.54 126.7 44.2 IGYSLGAHVA AGYAGNFVK_974.49_848.4 GYAGNFVK NETO1_HUMAN DGPFGFSPII DGPFGFSPIIGR_631.83_ 631.83 846.5 21.4 82.5 36.3 GR 846.5 CSF1R_HUMAN VIPGPPALTL VIPGPPALTLVPAELVR_ 581.36 846.5 24.37 76 24.2 VPAELVR 581.36_846.5 ATS1_HUMAN GPEVTSNAAL GPEVTSNAALTLR_664.86_ 664.86 845.5 14.03 86.8 40.8 TLR 845.5 APOB_HUMAN IEGNLIFDPN IEGNLIFDPNNYLPK_873.96 873.96 845.5 22.93 113.7 44 NYLPK _845.5 MTOR_HUMAN TLDQSPELR TLDQSPELR_529.78_844.4 529.78 844.4 10.35 69.3 25.8 ITA3_HUMAN TVEDVGSPLK TVEDVGSPLK_522.78_844.4 522.78 844.4 11.35 68.4 25.5 BTNL8_HUMAN VQIGDTFFEP VQIGDTFFEPISWHLATK_ 697.03 842.5 24.32 90.9 39.1 ISWHLATK 697.03_842.5 IPSP_HUMAN AAAATGTIFT AAAATGTIFTFR_613.83_ 613.83 841.5 18.99 80.2 29.5 FR 841.5 DBC1_HUMAN ISDVQVFGYS ISDVQVFGYSLR_692.37_ 692.37 841.5 19.96 90.3 33 LR 841.5 KLKB1_HUMAN YSPGGTPTAI YSPGGTPTAIK_546.29_841.5 546.29 841.5 9.73 71.5 26.5 K VTDB_HUMAN VPTADLEDVL VPTADLEDVLPLAEDITNIL 789.43 841.4 25.27 102.8 46.8 PLAEDITNIL SK_789.43_841.4 SK ADA12_HUMAN NHPEVLNIR NHPEVLNIR_546.3_840.5 546.3 840.5 12.38 71.5 29.5 GP125_HUMAN SYGLNLAIQN SYGLNLAIQNGPIK_744.41_ 744.41 840.5 18.45 97 35.3 GPIK 840.5 CAD22_HUMAN LWAAGTPSPS LWAAGTPSPSAPGAR_ 719.88 839.4 12.93 93.9 37.2 APGAR 719.88_839.4 MMP1_HUMAN SQNPVQPIGP SQNPVQPIGPQTPK_745.9_ 745.9 837.5 10.67 97.2 35.3 QTPK 837.5 SYTL4_HUMAN ISAESPTGLP ISAESPTGLPSHK_441.9_ 441.9 836.5 9.66 58 23.1 SHK 836.5 A1AG1_HUMAN YVGGQEHFAH YVGGQEHFAHLLILR_ 584.99 835.6 19.31 76.5 36.4 LLILR 584.99_835.6 PICAL_HUMAN ITAAQHSVTG ITAAQHSVTGSAVSK_ 486.26 835.5 7.72 63.7 28.3 SAVSK 486.26_835.5 CSF1R_HUMAN HTFTLSLPR HTFTLSLPR_536.3_833.5 536.3 833.5 16.88 70.2 29.1 CHAD_HUMAN YLETLWLDNT YLETLWLDNTNLEK_876.45 876.45 833.4 21.97 114.1 41.1 NLEK _833.4 NTRK2_HUMAN NLTIVDSGLK NLTIVDSGLK_530.31_832.5 530.31 832.5 15.17 69.4 25.8 ABCA1_HUMAN FVSPLSWDLV FVSPLSWDLVGR_688.37_ 688.37 832.4 23.76 89.8 38.8 GR 832.4 CAR14_HUMAN TASDQESGDE TASDQESGDEELNR_775.83 775.83 832.4 7.6 101.1 39.6 ELNR _832.4 XLRS1_HUMAN VISGILTQGR VISGILTQGR_522.31_831.5 522.31 831.5 14.23 68.4 25.5 GRM5_HUMAN DSLISSEEEE DSLISSEEEEGLVR_781.88_ 781.88 831.4 16 101.9 36.9 GLVR 831.4 LAT2_HUMAN SGGDYSYVK SGGDYSYVK_488.22_831.4 488.22 831.4 9.01 64 24 KCNA5_HUMAN NRPSFDGILY NRPSFDGILYYYQSGGR_ 664.99 830.4 20.98 86.8 31.5 YYQSGGR 664.99_830.4 KCTD4_HUMAN YPDTFLEGIV YPDTFLEGIVNGK_726.87_ 726.87 829.5 21.72 94.8 37.5 NGK 829.5 KCNA5_HUMAN EEQGTQSQGP EEQGTQSQGPGLDR_751.35 751.35 829.4 7.7 97.9 44.6 GLDR _829.4 PON1_HUMAN IQNILTEEPK IQNILTEEPK_592.8_829.4 592.8 829.4 14.9 77.5 28.6 NKX62_HUMAN LAGPAPAGGV LAGPAPAGGVLDK_583.33_ 583.33 827.5 12.29 76.2 31.2 LDK 827.5 A1AG1_HUMAN WFYIASAFR WFYIASAFR_580.8_827.4 580.8 827.4 23.48 75.9 25.1 AFAM_HUMAN DADPDTFFAK DADPDTFFAK_563.76_825.4 563.76 825.4 15.58 73.7 27.3 PARD3_HUMAN ESVSTASDQP ESVSTASDQPSHSLER_ 577.27 825.4 8.68 75.5 33 SHSLER 577.27_825.4 PEDF_HUMAN LSYEGEVTK LSYEGEVTK_513.26_825.4 513.26 825.4 10.07 67.2 25.1 MOT2_HUMAN LVDLTGEYK LVDLTGEYK_519.28_825.4 519.28 825.4 13.84 68 25.3 MMP17_HUMAN ESVSPTAQPE ESVSPTAQPEEPPLLPEPPD 800.4 824.4 16.38 104.3 38.4 EPPLLPEPPD NR_800.4_824.4 NR APOD_HUMAN IPTTFENGR IPTTFENGR_517.77_824.4 517.77 824.4 11.08 67.8 31.3 SYN2_HUMAN VLLVVDEPHA VLLVVDEPHADWAK_ 531.29 824.4 17.62 69.5 27.6 DWAK 531.29_824.4 LTBP1_HUMAN EEPVEALTFS EEPVEALTFSR_639.32_823.4 639.32 823.4 16.79 83.5 39.6 R LCK_HUMAN AQSLTTGQEG AQSLTTGQEGFIPFNFVAK_ 685.69 822.5 23.14 89.5 32.5 FIPFNFVAK 685.69_822.5 CEL3A_HUMAN EGPEQVIPIN EGPEQVIPINSEELFVHPLW 868.45 822.4 24.05 113 44.8 SEELFVHPLW NR_868.45_822.4 NR AACT_HUMAN EIGELYLPK EIGELYLPK_531.3_819.5 531.3 819.5 18.4 69.5 25.9 FGFR3_HUMAN VGPDGTPYVT VGPDGTPYVTVLK_673.37__ 673.37 819.5 15.91 87.9 35.1 VLK 819.5 CK041_HUMAN DVAQDGSTIK DVAQDGSTIK_517.26_819.4 517.26 819.4 7.48 67.7 25.3 A4_HUMAN LVFFAEDVGS LVFFAEDVGSNK_663.34_ 663.34 819.4 19.15 86.6 31.7 NK 819.4 PCDAA_HUMAN ALDETDSPR ALDETDSPR_502.24_819.3 502.24 819.3 7.31 65.8 27.6 ACHA5_HUMAN GLSEPSSIAK GLSEPSSIAK_494.77_818.4 494.77 818.4 10.19 64.8 24.3 KIF5A_HUMAN LYLVDLAGSE LYLVDLAGSEK_604.33_ 604.33 818.4 18.66 79 29.1 K 818.4 LRTM2_HUMAN LSALPSWAFA LSALPSWAFANLSSLQR_ 621 817.5 24.55 81.1 26.2 NLSSLQR 621_817.5 PCSK1_HUMAN AEAQEAEDQQ AEAQEAEDQQAR_673.3_ 673.3 817.4 6.08 87.9 35.1 AR 817.4 CD3D_HUMAN LSGAADTQAL LSGAADTQALLR_608.34_ 608.34 816.5 14.11 79.5 29.3 LR 816.5 ARP21_HUMAN TASFGGITVL TASFGGITVLTR_611.84_ 611.84 816.5 18.32 79.9 29.4 TR 816.5 MPDZ_HUMAN HVLEDSPAGK HVLEDSPAGK_526.77_816.4 526.77 816.4 7.15 69 28.7 KCC1G_HUMAN DIYPSVSLQI DIYPSVSLQIQK_695.88_ 695.88 815.5 17.14 90.8 42.1 QK 815.5 CSF1_HUMAN FNSVPLTDTG FNSVPLTDTGHER_491.58_ 491.58 815.4 11.74 64.4 28.6 HER 815.4 CADH5_HUMAN YTFVVPEDTR YTFVVPEDTR_613.8_815.4 613.8 815.4 16.3 80.2 32.5 PCDA5_HUMAN LLENAPSGTL LLENAPSGTLVIK_677.9_ 677.9 814.5 15.78 88.4 32.3 VIK 814.5 TRPV5_HUMAN AEGPGSQLQK AEGPGSQLQK_507.76_814.4 507.76 814.4 7.63 66.5 24.8 APOA1_HUMAN AKPALEDLR AKPALEDLR_506.79_813.4 506.79 813.4 11.56 66.4 24.8 TYRO_HUMAN DLGYDYSYLQ DLGYDYSYLQDSDPDSFQD 883.05 813.4 21.17 114.9 45.6 DSDPDSFQDY YIK_883.05_813.4 IK 41_HUMAN NFMESVPEPR NFMESVPEPR_603.3_813.4 603.3 813.4 16 78.8 32 C1QT4 HUMAN SLVGSDAGPG SLVGSDAGPGPR_556.79_ 556.79 813.4 9.81 72.8 30 PR 813.4 ITAM_HUMAN VQSLVLGAPR VQSLVLGAPR_520.32_812.5 520.32 812.5 13.93 68.1 25.4 GRM5_HUMAN IYSNAGEQSF IYSNAGEQSFDK_679.82_ 679.82 810.4 10.33 88.7 32.4 DK 810.4 T151A_HUMAN LFGASSPPPG LFGASSPPPGAVPSGPPLSR_ 946.01 810.4 16.44 123 44.1 AVPSGPPLSR 946.01_810.4 PKDRE_HUMAN VIVIPNPVDL VIVIPNPVDLR_617.88_810.4 617.88 810.4 20.18 80.7 29.7 R

SEPT3_HUMAN YINEQYEK YINEQYEK_543.76_810.4 543.76 810.4 8.49 71.1 26.4 HAS1_HUMAN ILNPLDSWVS ILNPLDSWVSFLSSLR_ 924.01 809.5 25.49 120.2 49.2 FLSSLR 924.01_809.5 SNTB1_HUMAN GSPQAGVDLS GSPQAGVDLSFATR_703.36 703.36 809.4 15.9 91.7 36.4 FATR 809.4 SLIK1_HUMAN SLPVDVFAGV SLPVDVFAGVSLSK_709.9_ 709.9 808.5 22.04 92.6 36.7 SLSK 808.5 RRAGC_HUMAN AFLFDVVSK AFLFDVVSK_513.29_807.5 513.29 807.5 21 67.2 25.1 OLIG1_HUMAN FPHLVPASLG FPHLVPASLGLAAVQAQFS 694.39 807.4 24.11 90.6 33 LAAVQAQFSK K_694.39_807.4 TLR7_HUMAN LQELDLSQNF LQELDLSQNFLAK_759.91_ 759.91 807.4 19.89 99 35.9 LAK 807.4 CEL3A_HUMAN VVHGEDAVPY VVHGEDAVPYSWPWQVSL 873.1 807.4 23.3 113.6 45.1 SWPWQVSLQY QYEK_873.1_807.4 EK LGI1_HUMAN WGGSSFQDIQ WGGSSFQDIQR_640.8_806.4 640.8 806.4 14.47 83.7 30.7 R SCN2A_HUMAN ALIESNQTAR ALIESNQTAR_551.8_805.4 551.8 805.4 8.92 72.2 26.8 ODP2_HUMAN LSEGDLLAEI LSEGDLLAEIETDK_766.89_ 766.89 805.4 20.65 99.9 36.2 ETDK 805.4 FGFR2_HUMAN DAAVISWTK DAAVISWTK_495.77_804.5 495.77 804.5 14.9 65 24.3 KCC1G_HUMAN DLKPENLLYL DLKPENLLYLTPEENSK_ 668.35 804.4 19.4 87.2 31.6 TPEENSK 668.35_804.4 KCC2A_HUMAN FYFENLWSR FYFENLWSR_631.3_804.4 631.3 804.4 23.34 82.4 30.3 41_HUMAN SLDGAAAVD SLDGAAAVDSADR_624.3_ 624.3 804.4 10.15 81.5 30 SADR 804.4 KAIN_HUMAN WADLSGITK WADLSGITK_495.77_804.4 495.77 804.4 15.63 65 24.3 JPH3_HUMAN WDLTFSPPQK WDLTFSPPQK_609.81_804.4 609.81 804.4 19.12 79.7 29.3 LSHR_HUMAN LSLAYLPVK LSLAYLPVK_502.31_803.5 502.31 803.5 18.88 65.8 24.6 KIRR2_HUMAN FLVETFPAPE FLVETFPAPESR_696.86_ 696.86 803.4 17.78 90.9 33.2 SR 803.4 EPHA8_HUMAN WTAPEAIAFR WTAPEATAFR_581.31_803.4 581.31 803.4 18.23 76 28.1 S39A4_HUMAN ALTPGLSWLL ALTPGLSWLLQR_677.9_ 677.9 802.5 24.56 88.4 38.3 QR 802.5 CLUS_HUMAN ELDESLQVAE ELDESLQVAER_644.8_802.4 644.8 802.4 13.4 84.2 36.9 R KIF5A_HUMAN TGAEGAVLDE TGAEGAVLDEAK_580.79_ 580.79 802.4 10.99 75.9 28.1 AK 802.4 CP46A_HUMAN AEQLVEILEA AEQLVEILEAK_621.85_ 621.85 801.5 20.05 81.2 29.9 K 801.5 NMDE1_HUMAN DEAVLILSEA DEAVLILSEAR_608.33_ 608.33 801.5 17.94 79.5 29.3 R 801.5 NCAN_HUMAN VEAHGEATAT VEAHGEATATAPPSPAAET 645.65 800.4 8.33 84.3 33.5 KAPPSPAAET K_645.65_800.4 ITIH2_HUMAN AHVSFKPTVA AHVSFKPTVAQQR_490.27_ 490.27 799.4 9.87 64.2 25.6 QQR 799.4 ITB5_HUMAN LGFGSFVDK LGFGSFVDK_485.26_799.4 485.26 799.4 17.93 63.6 23.9 C1QL2_HUMAN IAFYVGLK IAFYVGLK_455.77_797.5 455.77 797.5 18.6 59.8 22.6 LRP8_HUMAN LYWVDSK LYWVDSK_455.7_797.4 455.7 797.4 15.9 59.8 22.6 ITB2_HUMAN VFLDHNALPD VFLDHNALPDTLK_494.94_ 494.94 797.4 16.62 64.8 22.8 TLK 797.4 CACB2_HUMAN ATHPPSSSLP ATHPPSSSLPNPLLSR_ 558.64 796.5 15.74 73.1 26 NPLLSR 558.64_796.5 RES18_HUMAN GFSGPLPTVG GFSGPLPTVGR_544.3_796.5 544.3 796.5 15.06 71.2 26.4 R SEPP1_HUMAN LVYHLGLPFS LVYHLGLPFSFLTFPYVEEA 861.8 796.5 25.31 112.2 44.5 FLTFPYVEEA IK_861.8_796.5 IK A4_HUMAN WYFDVTEGK WYFDVTEGK_572.77_795.4 572.77 795.4 18.68 74.9 27.7 MMP16_HUMAN YGYLPPTDPR YGYLPPTDPR_589.8_795.4 589.8 795.4 12.72 77.1 28.5 EMIL2_HUMAN LDNEFDR LDNEFDR_454.71_795.3 454.71 795.3 10.18 59.7 22.5 NMDE1_HUMAN FTYDLYLVTN FTYDLYLVTNGK_717.4_ 717.4 794.4 22 93.5 37.1 GK 794.4 TR11B_HUMAN HIGHANLTFE HIGHANLTFEQLR_512.61_ 512.61 793.4 14.55 67.1 23.7 QLR 793.4 AP1B1_HUMAN LHDINAQLVE LHDINAQLVEDQGFLDTLK 723.71 793.4 21.38 94.4 34.5 DQGFLDTLK _723.71_793.4 SCG3_HUMAN LNVEDVDSTK LNVEDVDSTK_560.28_793.4 560.28 793.4 11 73.3 27.2 FGFR2_HUMAN EIEVLYIR EIEVLYIR_517.8_792.5 517.8 792.5 18.55 67.8 25.3 AP1B1_HUMAN LHDINAQLVE LHDINAQLVEDQGFLDTLK 723.71 792.4 21.38 94.4 34.5 DQGFLDTLK _723.71_792.4 APOE_HUMAN LQAEAFQAR LQAEAFQAR_517.28_792.4 517.28 792.4 11.18 67.7 25.3 NMDZ1_HUMAN VLQFDPGTK VLQFDPGTK_502.77_792.4 502.77 792.4 13.24 65.9 24.6 CO4B_HUMAN YIYGKPVQGV YIYGKPVQGVAYVR_538.3_ 538.3 792.4 13.9 70.4 34 AYVR 792.4 PAK3_HUMAN LLQTSNITK LLQTSNITK_509.3_791.4 509.3 791.4 10.64 66.7 24.9 SCG3_HUMAN ELSAERPLNE ELSAERPLNEQIAEAEEDK_ 724.35 791.3 15.56 94.4 34.5 QIAEAEEDK 724.35_791.3 LAT2_HUMAN DIFGGLAGFL DIFGGLAGFLR_583.32_ 583.32 790.5 25.16 76.2 28.2 R 790.5 UGGG2_HUMAN INEENTAISR INEENTAISR_573.79_790.4 573.79 790.4 8.3 75 36.7 PLCB1_HUMAN ISEDSNHGSA ISEDSNHGSAPLSLSSDPGK 666.65 790.4 11.82 87 31.5 PLSLSSDPGK _666.65_790.4 NETR_HUMAN VSAFVPWIK VSAFVPWIK_523.81_789.5 523.81 789.5 21.34 68.6 25.5 CERU_HUMAN GPEEEHLGIL GPEEEHLGILGPVIWAEVG 829.77 789.4 25.08 108 39.9 GPVIWAEVGD DTIR_829.77_789.4 TIR PVRL1_HUMAN ITQVTWQK ITQVTWQK_502.28_789.4 502.28 789.4 11.81 65.8 24.6 AP1B1_HUMAN LLSTDPVAAK LLSTDPVAAK_507.8_788.4 507.8 788.4 11.18 66.5 24.8 APOA2_HUMAN SPELQAEAK SPELQAEAK_486.75_788.4 486.75 788.4 7.93 63.8 26.9 MTOR_HUMAN LIHQLLTDIG LIHQLLTDIGR_639.9_787.4 639.9 787.4 19.8 83.5 36.7 R SEPP1_HUMAN LPTDSELAPR LPTDSELAPR_549.79_787.4 549.79 787.4 11.86 71.9 35.7 E41L3_HUMAN TETISFGSVS TETISFGSVSPGGVK_733.38 733.38 787.4 16.02 95.6 34.8 PGGVK _787.4 FGL1_HUMAN HGEYWLGNK HGEYWLGNK_552.27_786.4 552.27 786.4 12.65 72.2 29.8 ITIH2_HUMAN FYNQVSTPLL FYNQVSTPLLR_669.4_785.5 669.4 785.5 18.3 87.3 35 R CO3_HUMAN LSINTHPSQK LSINTHPSQKPLSITVR_ 631.03 785.5 14.39 82.4 32.7 PLSITVR 631.03_785.5 ABCA1_HUMAN EGAFVELFHE EGAFVELFHEIDDR_559.6_ 559.6 784.4 23.86 73.2 35.1 IDDR 784.4 AT2B3_HUMAN ILDLLVHAIS ILDLLVHAISINSAYTTK_ 658.04 784.4 24.28 85.9 31.1 INSAYTTK 658.04_784.4 T151A_HUMAN LVLGPEAALA LVLGPEAALAR_555.34_ 555.34 784.4 16.86 72.6 29.9 R 784.4 APOA4_HUMAN LVPFATELHE LVPFATELHER_437.9_784.3 437.9 784.3 17.2 57.5 25.9 R E41L3_HUMAN IRPGEFEQFE IRPGEFEQFESTIGFK_628.99 628.99 781.4 20.89 82.1 29.6 STIGFK _781.4 GP158_HUMAN LLGFATVYGT LLGFATVYGTVTLK_741.93 741.93 781.4 23.26 96.7 35.1 VTLK _781.4 GELS_HUMAN TASDFITK TASDFITK_441.73_781.4 441.73 781.4 10.75 58 21.9 NMS_HUMAN TQEATHPVK TQEATHPVK_505.77_781.4 505.77 781.4 5.87 66.2 27.8 MMP16_HUMAN ILLTFSTGR ILLTFSTGR_504.3_781.3 504.3 781.3 18.9 66.1 27.7 ITAM_HUMAN LFTALFPFEK LFTALFPFEK_606.84_780.4 606.84 780.4 24.87 79.3 29.2 GPC6_HUMAN SAPENFNTR SAPENFNTR_518.25_780.4 518.25 780.4 8.44 67.9 31.3 AGAL_HUMAN SILDWTSFNQ SILDWTSFNQER_748.36_ 748.36 780.4 21.18 97.5 38.4 ER 780.4 CO1A2_HUMAN GEAGAAGPAG GEAGAAGPAGPAGPR_ 618.31 779.4 7.34 80.8 35.7 PAGPR 618.31_779.4 TAU_HUMAN TDHGAEIVYK TDHGAEIVYK_566.79_779.4 566.79 779.4 9.06 74.1 30.4 LRTM2_HUMAN LDLSNNFLDR LDLSNNFLDR_603.81_778.4 603.81 778.4 18.13 78.9 29.1 GP113_HUMAN WNLYEVVR WNLYEVVR_539.79_778.4 539.79 778.4 20.42 70.6 26.3 RUN3A_HUMAN FTQSYDYLTD FTQSYDYLTDEEER_898.39_ 898.39 778.3 15.16 116.9 42 EEER 778.3 TBB2B_HUMAN YLTVAAIFR YLTVAAIFR_527.31_777.5 527.31 777.5 22.63 69 25.7 GP2_HUMAN NTLSLVNDFI NTLSLVNDFIIR_702.9_777.4 702.9 777.4 24.08 91.7 33.4 IR ITIH4_HUMAN GPDVLTATVS GPDVLTATVSGK_572.81_ 572.81 776.5 13.31 74.9 27.7 GK 776.5 INHBA_HUMAN GHSPFANLK GHSPFANLK_485.76_776.4 485.76 776.4 12.42 63.7 23.9 TERA_HUMAN GILLYGPPGT GILLYGPPGTGK_586.84_ 586.84 776.4 16.52 76.7 28.3 GK 776.4 TNR19_HUMAN IASTASSPR IASTASSPR_445.24_776.4 445.24 776.4 6.17 58.4 25.1 TR11B_HUMAN NGDQDTLK NGDQDTLK_445.71_776.4 445.71 776.4 6.5 58.5 22.1 PDIA2_HUMAN ADFPVDEELG ADFPVDEELGLDLGDLSR_ 980.98 775.4 23.42 127.5 42.7 LDLGDLSR 980.98_775.4 GRN_HUMAN EVVSAQPATF EVVSAQPATFLAR_694.88_ 694.88 775.4 16.12 90.6 33.1 LAR 775.4 ITIH1_HUMAN FAHYVVTSQV FAHYVVTSQVVNTANEAR_ 669.34 775.4 14.4 87.3 31.7 VNTANEAR 669.34_775.4 APOA4_HUMAN ISASAEELR ISASAEELR_488.26_775.4 488.26 775.4 10.18 64 24 AACT_HUMAN ITLLSALVET ITLLSALVETR_608.37_775.4 608.37 775.4 23.62 79.5 29.3 R HEMO_HUMAN NFPSPVDAAF NFPSPVDAAFR_610.81_ 610.81 775.4 17.76 79.8 35.4 R 775.4 LGMN_HUMAN QLTEEIQR QLTEEIQR_508.77_775.4 508.77 775.4 9.87 66.6 33.9 TERA_HUMAN GGNIGDGGGA GGNIGDGGGAADR_558.76_ 558.76 775.3 6.49 73.1 27.1

ADR 775.3 MICA_HUMAN AKPQGQWAED AKPQGQWAEDVLGNK_ 547.62 774.4 15.39 71.6 25.5 VLGNK 547.62_774.4 KCNA5_HUMAN NRPSFDGILY NRPSFDGILYYYQSGGR_ 664.99 774.4 20.98 86.8 34.5 YYQSGGR 664.99_774.4 CNTN2_HUMAN VISDTEADIG VISDTEADIGSNLR_745.38_ 745.38 774.4 13.74 97.2 35.3 SNLR 774.4 JPH3_HUMAN AEAALTAAQK AEAALTAAQK_487.27_ 487.27 773.5 7.79 63.9 23.9 773.5 KCNA1_HUMAN VVINISGLR VVINISGLR_485.81_772.5 485.81 772.5 17.1 63.7 23.9 SCNNA_HUMAN DNNPQVDWK DNNPQVDWK_558.26_772.4 558.26 772.4 11.27 73 27.1 CO4A_HUMAN GSFEFPVGD GSFEFPVGDAVSK_670.33_ 670.33 772.4 17.53 87.5 32 AVSK 772.4 APOD_HUMAN VLNQELR VLNQELR_436.2_772.4 436.2 772.4 8.4 57.3 24.7 FCGRN_HUMAN SGLPAPWIS SGLPAPWISLR_598.84_771.5 598.84 771.5 21.69 78.3 31.8 LR FCGRN_HUMAN GDDTGVLLP GDDTGVLLPTPGEAQDADL 1006.49 771.4 16.76 130.8 43.8 TPGEAQDAD K_1006.49_771.4 LK APOC2_HUMAN TYLPAVDEK TYLPAVDEK_518.27_771.4 518.27 771.4 12.36 67.9 25.3 RRAGC_HUMAN VDGLSDDHK VDGLSDDHK_493.23_771.4 493.23 771.4 6.78 64.6 27.2 ACHB_HUMAN VPETSLSVP VPETSLSVPIIIK_698.43_ 698.43 769.5 19.99 91.1 36.2 IIIK 769.5 NRG3_HUMAN DLVGYSSTR DLVGYSSTR_499.25_769.4 499.25 769.4 10.78 65.4 24.5 VTNC_HUMAN IYISGMAPR IYISGMAPRPSLAK_501.9_ 501.9 768.5 22.5 65.8 26.1 PSLAK 768.5 GRIA2_HUMAN FAYLYDSDR FAYLYDSDR_575.26_768.4 575.26 768.4 14.83 75.2 27.8 TADBP_HUMAN FTEYETQVK FYEYETQVK_572.78_767.4 572.78 767.4 10.48 74.9 27.7 SO1B3_HUMAN GIGETPIVP GIGETPIVPLGISYIDDFAK_ 1053.06 767.4 25.06 136.8 45.8 LGISYIDDF 1053.06_767.4 AK PCDGE_HUMAN VTDANDNPP VTDANDNPPVFSQDVYR_ 646.31 767.4 15.33 84.4 30.5 VFSQDVYR 646.31_767.4 PERL_HUMAN FWWENPGVF FWWENPGVFTNEQK_ 891.42 766.4 22.67 116 38.7 TNEQK 891.42_766.4 I12R1_HUMAN AGVAYTVQV AGVAYTVQVR_532.3_765.4 532.3 765.4 12.09 69.7 25.9 R APOA4_HUMAN LEPYADQLR LEPYADQLR_552.79_765.4 552.79 765.4 12.59 72.3 32.8 IL1AP_HUMAN LYIEYGIQR LYIEYGIQR_577.81_765.4 577.81 765.4 15.79 75.5 27.9 PGCP_HUMAN AIINLAVYG AIINLAVYGK_531.32_764.4 531.32 764.4 17.88 69.5 22.9 K PGCB_HUMAN ELEAPSEDN ELEAPSEDNSGR_652.29_ 652.29 764.3 8.33 85.1 37.2 SGR 764.3 MTOR_HUMAN VLGLLGALD VLGLLGALDPYK_629.87_ 629.87 763.4 24.65 82.3 30.2 PYK 763.4 DBC1_HUMAN VNHALDLYN VNHALDLYNTEILK_548.3_ 548.3 763.4 17.87 71.7 25.5 TEILK 763.4 ACHA5_HUMAN EETESGSGP EETESGSGPK_510.73_762.4 510.73 762.4 5.83 66.9 28 K PTPRO_HUMAN IANLLPAWY IANLLPAWYYNFR_547.62_ 547.62 762.4 24.56 71.6 25.5 YNFR 762.4 ICAM3_HUMAN WEEELSR WEEELSR_474.72_762.4 474.72 762.4 11.56 62.2 32.4 KCNV2_HUMAN FSDYYSK FSDYYSK_455.2_762.3 455.2 762.3 9.95 59.7 22.5 PAR6B_HUMAN FEEFYGLLQ FEEFYGLLQHVHK_549.62_ 549.62 761.4 19.68 71.9 31.6 HVHK 761.4 GRIA2_HUMAN GYHYIIANL GYHYIIANLGFTDGDLLK_ 670.68 761.4 23.39 87.5 31.8 GFTDGDLLK 670.68_761.4 ZIC2_HUMAN LGLPGEVFGR LGLPGEVFGR_522.8_761.4 522.8 761.4 18.49 68.4 25.5 PCDBC_HUMAN VGATDHGSPA VGATDHGSPALSSEALVR_ 589.64 761.4 13.35 77.1 27.6 LSSEALVR 589.64_761.4 WNT8B_HUMAN VDLLQGAGNS VDLLQGAGNSAAGR_ 664.85 760.4 13.4 86.8 31.8 AAGR 664.85_760.4 DDR2_HUMAN NLYSGDYYR NLYSGDYYR_575.76_760.3 575.76 760.3 12.58 75.3 27.8 NMDZ1_HUMAN IPVLGLTTR IPVLGLTTR_485.31_759.5 485.31 759.5 17.9 63.6 23.9 CETP_HUMAN ASYPDITGEK ASYPDITGEK_540.76_759.4 540.76 759.4 11.23 70.8 26.3 SHSA7_HUMAN LVSQEHLLLS LVSQEHLLLSSPEALR_598_ 598 759.4 17.69 78.1 34 SPEALR 759.4 PTPRB_HUMAN SLVVSWSPPA SLVVSWSPPAGDWEQYR_ 988.98 759.4 21.21 128.6 43 GDWEQYR 988.98_759.4 KSYK_HUMAN YLEESNFVHR YLEESNFVHR_431.88_759.4 431.88 759.4 12.59 56.7 22.6 MPDZ_HUMAN SLGISIVGGR SLGISIVGGR_479.79_758.5 479.79 758.5 15.92 62.9 23.6 NETR_HUMAN WAEVPPFLER WAEVPPFLER_622.33_758.4 622.33 758.4 20.93 81.3 29.9 KSYK_HUMAN LIATTAHEK LIATTAHEK_492.28_757.4 492.28 757.4 6.84 64.5 24.2 NKX62_HUMAN LAGPAPAGGV LAGPAPAGGVLDK_583.33_ 583.33 756.4 12.29 76.2 31.2 LDK 756.4 OTOAN_HUMAN THSWQDAPAS THSWQDAPASAGPTR_ 527.92 756.4 9.56 69.1 24.5 AGPTR 527.92_756.4 MOT2_HUMAN YLDFSLFK YLDFSLFK_516.77_756.4 516.77 756.4 23.12 67.7 25.2 HCN2_HUMAN QFGALLQPGV QFGALLQPGVNK_636.36_ 636.36 755.4 16.27 83.1 30.5 NK 755.4 KALRN_HUMAN LTLSDFLIKP LTLSDFLIKPIQR_515.31_ 515.31 754.5 23.26 67.5 32.8 IQR 754.5 GABR2_HUMAN TVPSDNAVNP TVPSDNAVNPAILK_719.9_ 719.9 754.5 14.05 93.9 40.2 AILK 754.5 IGF1R_HUMAN AENGPGPGVL AENGPGPGVLVLR_639.86_ 639.86 753.5 16.44 83.5 39.7 VLR 753.5 CEL3B_HUMAN EGPEQVIPIN EGPEQVIPINSGDLFVHPLW 839.77 753.4 23.81 109.3 37.4 WSGDLFVHPL NR_839.77_753.4 NR TRFR_HUMAN ILFLNPIPSD ILFLNPIPSDPK_677.39_753.4 677.39 753.4 21.23 88.4 32.3 PK SHSA7_HUMAN SPALPPDPTA SPALPPDPTAR_561.3_753.4 561.3 753.4 10.3 73.4 27.2 R YQ051_HUMAN GGVDLPATPG GGVDLPATPGGPR_597.32_ 597.32 752.4 12.41 78.1 28.8 GPR 752.4 FEZ1_HUMAN VPTLLTDYIL VPTLLTDYILK_638.38_752.4 638.38 752.4 23.17 83.4 33.6 K ITIH3_HUMAN DYIFGNYIER DYIFGNYIER_645.31_751.4 645.31 751.4 19.24 84.2 30.9 PRIO_HUMAN QHTVTTTTK QHTVTTTTK_508.77_751.4 508.77 751.4 5.87 66.6 24.9 CETP_HUMAN VIQTAFQR VIQTAFQR_481.77_750.4 481.77 750.4 10.75 63.1 26.7 PTPRT_HUMAN VTLIETEPLA VTLIETEPLAEYVIR_582.66_ 582.66 750.4 23 76.2 27.3 EYVIR 750.4 ARSE_HUMAN VVHHDPPLLF VVHHDPPLLFDLSR_548.97_ 548.97 750.4 19.49 71.8 25.5 DLSR 750.4 TRPV5_HUMAN ASLALPTSSL ASLALPTSSLSR_601.84_ 601.84 747.4 15.54 78.6 29 SR 747.4 BDNF_HUMAN GLTSLADTFE GLTSLADTFEHVIEELLDED 834.75 747.4 25.61 108.7 37.1 HVIEELLDED QK_834.75_747.4 QK PACN1_HUMAN NNLLNEDLEK NNLLNEDLEK_601.31_747.4 601.31 747.4 14.37 78.6 29 CADH3_HUMAN STGTISVISS STGTISVISSGLDR_696.87_ 696.87 747.4 16.02 90.9 33.2 GLDR 747.4 CACB2_HUMAN TLATSSLPLS TLATSSLPLSPTLASNSQGS 839.42 747.3 16.73 109.3 37.4 PTLASNSQGS QGDQR_839.42_747.3 QGDQR NPTX1_HUMAN FQLTFPLR FQLTFPLR_511.3_746.5 511.3 746.5 21.84 67 25 GP125_HUMAN WDFDLLNGQG WDFDLLNGQGGWK_768.37 768.37 746.4 23.77 100.1 36.3 GWK _746.4 PCSK1_HUMAN AEAQEAEDQQ AEAQEAEDQQAR_673.3_ 673.3 746.3 6.08 87.9 35.1 AR 746.3 SREC_HUMAN GTQGSELDPK GTQGSELDPK_516.25_745.4 516.25 745.4 7.72 67.6 25.2 NMDE1_HUMAN GVEDALVSLK GVEDALVSLK_515.79_745.4 515.79 745.4 16.75 67.5 25.2 ODP2_HUMAN ILVAEGTR ILVAEGTR_429.76_745.4 429.76 745.4 9.62 56.4 24.4 GNAQ_HUMAN YYLNDLDR YYLNDLDR_536.26_745.4 536.26 745.4 14.39 70.2 26.1 APLP1_HUMAN DELAPAGTGV DELAPAGTGVSR_586.8_ 586.8 744.4 9.53 76.7 28.3 SR 744.4 GABR2_HUMAN NVSIPALNDS NVSIPALNDSK_579.31_ 579.31 744.4 14.46 75.7 28 K 744.4 TMPS2_HUMAN TSEVLNAAK TSEVLNAAK_466.76_744.4 466.76 744.4 8.64 61.2 23 KLKB1_HUMAN YSPGGTPTAI YSPGGTPTAIK_546.29_744.4 546.29 744.4 9.73 71.5 32.5 K IGF1R_HUMAN TTINNEYNYR TTINNEYNYR_644.3_744.3 644.3 744.3 9.54 84.1 30.8 PGCP_HUMAN LALLVDTVGP LALLVDTVGPR_577.35_ 577.35 743.4 18.84 75.5 27.9 R 743.4 SIG12_HUMAN NIPVATNNPA NIPVATNNPAR_583.82_ 583.82 743.4 9.32 76.3 37.2 R 743.4 DBC1_HUMAN ISDVQVFGYS ISDVQVFGYSLR_692.37_ 692.37 742.4 19.96 90.3 33 LR 742.4 CFAB_HUMAN YGLVTYATYP YGLVTYATYPK_638.33_ 638.33 742.4 15.87 83.3 30.6 K 742.4 CO4A4_HUMAN ADLQFSSAPA ADLQFSSAPAPDTLK_780.9 780.9 741.4 15.27 101.7 36.9 PDTLK _741.4 ACBD7_HUMAN ARPDDGELK ARPDDGELK_500.76_741.3 500.76 741.3 6.72 65.6 30.5 GOPC_HUMAN EHGVPILISE EHGVPILISEIHPGQPADR_ 689.03 740.4 17.44 89.9 41.7 IHPGQPADR 689.03_740.4 PKDRE_HUMAN GAQAAVSGAP GAQAAVSGAPGGLLR_ 662.87 740.4 14.12 86.5 31.7 GGLLR 662.87_740.4 RCN1_HUMAN HLVYESDK HLVYESDK_495.75_740.3 495.75 740.3 7.73 65 27.3 PZP_HUMAN AVGYLITGYQ AVGYLITGYQR_620.84_ 620.84 737.4 15.85 81.1 32.8

R 737.4 MMP20_HUMAN LAQAYLDK LAQAYLDK_461.26_737.4 461.26 737.4 10.6 60.5 22.8 P2RX1_HUMAN NLSPGFNFR NLSPGFNFR_526.27_737.4 526.27 737.4 16.65 68.9 25.7 SCRTl1HUMAN SDLGAPLHDK SDLGAPLHDK_526.77_737.4 526.77 737.4 9.54 69 31.7 CO9A2_HUMAN GEQGPPGIPG GEQGPPGIPGPQGLPGVK_ 842.95 736.4 16.02 109.7 39.6 PQGLPGVK 842.95_736.4 IL1R2_HUMAN QEYSENNENY QEYSENNENYIEVPLIFDPV 890.43 734.4 24.62 115.9 40 IEVPLIFDPV TR_890.43_734.4 TR ANO3_HUMAN AEQVNTEENK AEQVNTEENK_581.27_734.3 581.27 734.3 6.06 76 28.1 WNK4_HUMAN VTSVSDQNDR VTSVSDQNDR_560.77_734.3 560.77 734.3 6.17 73.3 27.2 FGFR2_HUMAN DAAVISWTK DAAVISWTK_495.77_733.4 495.77 733.4 14.9 65 24.3 ACE2_HUMAN SGENPYASID SGENPYASIDISK_690.84_ 690.84 733.4 13.96 90.1 38.9 ISK 733.4 41_HUMAN SLDGAAAVDS SLDGAAAVDSADR_624.3_ 624.3 733.3 10.15 81.5 30 ADR 733.3 PCSK1_HUMAN ALAHLLEAER ALAHLLEAER_374.88_730.4 374.88 730.4 14.09 49.4 19.7 SYN2_HUMAN TPALSPQRPL TPALSPQRPLTTQQPQSGTL 750.41 730.4 12.34 97.8 44.8 TTQQPQSGTL K_750.41_730.4 K A1BG_HUMAN ATWSGAVLAG ATWSGAVLAGR_544.8_ 544.8 730.3 15.9 71.3 32.5 R 730.3 PCDGE_HUMAN VTDANDNPPV VTDANDNPPVFSQDVYR_ 646.31 730.3 15.33 84.4 30.5 FSQDVYR 646.31_730.3 APC2_HUMAN LAAQEGPLSL LAAQEGPLSLSR_621.35_ 621.35 729.4 14.4 81.2 32.8 SR 729.4 SNTB1_HUMAN SGLLEVLVR SGLLEVLVR_493.31_728.5 493.31 728.5 21.74 64.6 24.2 SEM4A_HUMAN TLGSQPVLK TLGSQPVLK_471.78_728.4 471.78 728.4 10.96 61.9 23.3 FRS2_HUMAN FVLGPTPVQK FVLGPTPVQK_543.32_726.4 543.32 726.4 14.71 71.1 26.4 PZP_HUMAN LEAGINQLSF LEAGINQLSFPLSSEPIQGSY 1203.62 726.4 21.13 156.3 64.5 PLSSEPIQGS R_1203.62_726.4 YR SO1A2_HUMAN IYDSTTFR IYDSTTFR_501.75_726.3 501.75 726.3 11.46 65.7 24.6 CO8A_HUMAN LYYGDDEK LYYGDDEK_501.72_726.3 501.72 726.3 9.1 65.7 24.6 PAK3_HUMAN LAKPLSSLTP LAKPLSSLTPLIIAAK_818.52 818.52 725.5 21.28 106.6 47.5 LIIAAK _725.5 ANGT_HUMAN DPTFIPAPIQ DPTFIPAPIQAK_649.36_ 649.36 724.4 16.67 84.8 31.1 AK 724.4 FIBA_HUMAN TVIGPDGHK TVIGPDGHK_462.25_723.4 462.25 723.4 6.93 60.6 22.8 TMPS2_HUMAN VISHPNYDSK VISHPNYDSK_580.29_723.3 580.29 723.3 7.45 75.9 34 MMP24_HUMAN AAPGPPPPPP AAPGPPPPPPPPGQAPR_ 534.29 722.4 9.77 69.9 24.8 PPGQAPR 534.29_722.4 CAD19_HUMAN AVEPESEFVI AVEPESEFVIK_624.33_722.4 624.33 722.4 15.88 81.5 39 K PIN1_HUMAN EEALELINGY EEALELINGYIQK_507.27_ 507.27 722.4 20.61 66.4 23.4 IQK 722.4 CO1A2_HUMAN GEAGAAGPAG GEAGAAGPAGPAGPR_ 618.31 722.4 7.34 80.8 29.7 PAGPR 618.31_722.4 FGL1_HUMAN IDLADFEK IDLADFEK_475.75_722.4 475.75 722.4 17.24 62.4 23.4 CF072_HUMAN VFFPVSEYK VFFPVSEYK_558.29_722.4 558.29 722.4 17.92 73 27.1 COIA1_HUMAN AVGLAGTFR AVGLAGTFR_446.26_721.4 446.26 721.4 14.22 58.6 22.1 SEPT3_HUMAN STLVNTLFK STLVNTLFK_511.8_721.4 511.8 721.4 19.5 67 25 SEMG1_HUMAN DVSQSSIYSQ DVSQSSIYSQTEEK_800.87_ 800.87 721.3 10.52 104.3 37.7 TEEK 721.3 ACHA2_HUMAN SEDADSSVK SEDADSSVK_469.21_721.3 469.21 721.3 6 61.5 23.1 ALBU_HUMAN TYETTLEK TYETTLEK_492.75_720.4 492.75 720.4 9.43 64.6 24.2 KNG2_HUMAN YFIDFVAR YFIDFVAR_515.8_720.4 515.8 720.4 22.5 67.5 28.2 OLFL3_HUMAN TEADTISGR TEADTISGR_475.23_719.4 475.23 719.4 7.26 62.3 23.4 APOA1_HUMAN LAEYHAK LAEYHAK_416.22_718.4 416.22 718.4 6.59 54.7 20.8 KCTD4_HUMAN NGELLLPEGF NGELLLPEGFR_622.84_ 622.84 718.4 19.68 81.3 29.9 R 718.4 INADL_HUMAN SLVADGVAER SLVADGVAER_508.77_717.4 508.77 717.4 10.85 66.6 24.9 NCKX2_HUMAN TLLDLNDK TLLDLNDK_466.26_717.4 466.26 717.4 15.03 61.1 23 CRLF1_HUMAN VGGLEDQLSV VGGLEDQLSVR_586.82_ 586.82 717.4 14.23 76.7 31.3 R 717.4 DBC1_HUMAN VNHALDLYNT VNHALDLYNTEILK_548.3_ 548.3 717.4 17.87 71.7 25.5 EILK 717.4 PSMG1_HUMAN TSESTGSLPS TSESTGSLPSPFLR_739.88_ 739.88 716.4 17.54 96.4 35.1 PFLR 716.4 CADH5_HUMAN YTFVVPEDTR YTFVVPEDTR_613.81_716.4 613.81 716.4 15.34 80.2 29.5 IL1AP_HUMAN QIQVFEDEPA QIQVFEDEPAR_666.33_ 666.33 716.3 14.23 87 37.8 R 716.3 CAD19_HUMAN VSDINDNEPK VSDINDNEPK_565.77_716.3 565.77 716.3 7.2 74 27.4 ITIH2_HUMAN IQPSGGTNIN IQPSGGTNINEALLR_791.93 791.93 715.4 15.42 103.2 46.3 EALLR 715.4 ERMIN_HUMAN ITEQPLK I TQPLK_414.75_715.4 414.75 715.4 8.64 54.5 20.7 CO6_HUMAN ENPAVIDFEL ENPAVIDFELAPIVDLVR_ 1005.55 714.5 25.18 130.7 49.7 AVPIDLVR 1005.55_714.5 ITIH1_HUMAN EVAFDLEIPK EVAFDLEIPK_580.81_714.4 580.81 714.4 19.77 75.9 28.1 CAMKV_HUMAN LENLVYYNR LENLVYYNR_592.31_714.4 592.31 714.4 14.32 77.4 31.6 CSF1_HUMAN FNSVPLTDTG FNSVPLTDTGHER_491.58_ 491.58 714.3 11.74 64.4 31.6 HER 714.3 FIBA_HUMAN NSLFEYQK NSLFEYQK_514.76_714.3 514.76 714.3 14.01 67.4 25.1 PON1_HUMAN SFNPNSPGK SFNPNSPGK_474.23_713.4 474.23 713.4 7.72 62.2 23.4 F13A_HUMAN STVLTIPEII STVLTIPEIIIK_663.9_712.5 663.9 712.5 23.2 86.6 31.7 IK 41_HUMAN APIAAPEPEL APIAAPEPELK_568.32_712.4 568.32 712.4 12.69 74.3 27.5 K HEP2_HUMAN FAFNLYR FAFNLYR_465.75_712.4 465.75 712.4 19.02 61.1 23 AT2B3_HUMAN NVYDSISK NVYDSISK_463.24_712.4 463.24 712.4 10.5 60.8 22.9 S15A2_HUMAN FVNTLHK FVNTLHK_429.75_711.4 429.75 711.4 8.42 56.4 21.4 TRIM9_HUMAN NILVQTPESE NILVQTPESESPQSHR_ 607.97 711.4 11.95 79.4 37.6 SPQSHR 607.97_711.4 EDNRB_HUMAN SLAPAEVPK SLAPAEVPK_456.26_711.4 456.26 711.4 10.48 59.9 22.6 KAIN_HUMAN VGSALFLSHN VGSALFLSHNLK_429.2_ 429.2 711.4 17.1 56.4 25.4 LK 711.4 XLRS1_HUMAN VFYGNSDR VFYGNSDR_479.23_711.3 479.23 711.3 8.8 62.8 23.6 CP46A_HUMAN ALQTVFGER ALQTVFGER_510.78_708.4 510.78 708.4 14.69 66.9 25 CO4B_HUMAN LQETSNWLLS LQETSNWLLSQQQADGSFQ 1033.18 708.4 21.19 134.3 50.2 QQQADGSFQD DLSPVIHR_1033.18_708.4 LSPVIHR CEL3A_HUMAN VVHGEDAVPY VVHGEDAVPYSWPWQVSL 873.1 708.3 23.3 113.6 51.1 SWPWQVSLQY QYEK_873.1_708.3 EK UNC5C_HUMAN DFESDIIDSS DFESDIIDSSALNGGFQPVNI 789.39 707.3 22.67 102.8 37.8 ALNGGFQPVN K_789.39_707.3 IK HEP2_HUMAN IAIDLFK IAIDLFK_410.25_706.4 410.25 706.4 19.9 53.9 20.6 CADH7_HUMAN VVYSILQGQP VVYSILQGQPYFSVEPK_ 652.02 706.4 21.48 85.1 30.8 YFSVEPK 652.02_706.4 CRUM1_HUMAN EPEFLNISIQ EPEFLNISIQDSR_774.39_ 774.39 705.4 19.34 100.9 36.6 DSR 705.4 TNR19_HUMAN IASTASSPR IASTASSPR_445.24_705.4 445.24 705.4 6.17 58.4 22.1 RGS4_HUMAN LGFLLQK LGFLLQK_409.76_705.4 409.76 705.4 18.02 53.9 20.5 NOE3_HUMAN SIADFVSGAE SIADFVSGAESR_619.8_ 619.8 705.4 16.43 81 29.8 SR 705.4 ANT3_HUMAN DDLYVSDAFH DDLYVSDAFHK_655.31_ 655.31 704.3 14.4 85.5 34.3 K 704.3 A1AG2_HUMAN NWGLSFYADK NWGLSFYADKPETTK_ 586.29 703.4 18.43 76.6 36.5 PETTK 586.29_703.4 668.35 703.3 19.4 87.2 31.6 KCC1G_HUMAN DLKPENLLYL DLKPENLLYLTPEENSK_ TPEENSK 668.35_703.3 INADL_HUMAN DVQPGSVADR DVQPGSVADR_522.26_701.4 522.26 701.4 7.7 68.4 25.5 CACB2_HUMAN GVGVVDQALR GVGVVDQALR_507.3_701.4 507.3 701.4 11.8 66.4 27.8 NOE3_HUMAN ITGPVTVK ITGPVTVK_407.76_701.4 407.76 701.4 10.07 53.6 20.4 S12A6_HUMAN LNEVIVNK LNEVIVNK_464.78_701.4 464.78 701.4 10.4 61 23 CD3D_HUMAN LSGAADTQAL LSGAADTQALLR_608.34_ 608.34 701.4 14.11 79.5 38.3 LR 701.4 CLUS_HUMAN EIQNAVNGVK EIQNAVNGVK_536.3_701.3 536.3 701.3 7.9 70.2 32.1 VTDB_HUMAN VLEPTLK VLEPTLK_400.25_700.4 400.25 700.4 11.66 52.6 20.1 AMFR2_HUMAN VPLDLSPR VPLDLSPR_448.76_700.4 448.76 700.4 13.3 58.9 22.2 NCKX2_HUMAN VTAPEAQAKP VTAPEAQAKPSAAR_466.26 466.26 700.4 6.95 61.1 30.3 SAAR _700.4 RELN_HUMAN FVYLELPAAA FVYLELPAAAK_611.35_ 611.35 699.4 19.69 79.9 29.4 K 699.4 PARD3_HUMAN TPIIWFHVVP TPIIWFHVVPAANK_531.64_ 531.64 698.4 21.26 69.6 24.7 AANK 698.4 SO1A2_HUMAN YIYLGLPAAL YIYLGLPAALR_625.37_ 625.37 697.4 22.53 81.7 30 R 697.4 CNTN1_HUMAN AHSDGGDGVV AHSDGGDGVVSQVK_ 452.56 697.3 8.14 59.4 20.6 SQVK 452.56_697.3 GALR3_HUMAN EGPVHGGEAA EGPVHGGEAAR_540.27_ 540.27 697.3 6.1 70.7 35.3 R 697.3 IC1_HUMAN TLYSSSPR TLYSSSPR_455.74_696.3 455.74 696.3 8.24 59.8 22.6

SEPT3_HUMAN YINEQYEK YINEQYEK_543.76_696.3 543.76 696.3 8.49 71.1 26.4 ITIH2_HUMAN SSALDMENFR SSALDMENFR_585.3_696.2 585.3 696.2 16.3 76.5 34.3 RRAGC_HUMAN AFLFDVVSK AFLFDVVSK_513.29_694.4 513.29 694.4 21 67.2 25.1 TADBP_HUMAN FGGNPGGFGN FGGNPGGFGNQGGFGNSR_ 863.89 694.3 14.09 112.4 46.5 QGGFGNSR 863.89_694.3 GT253_HUMAN NLGVDLLPGY NLGVDLLPGYQDPYSGR_ 621.98 694.3 20.39 81.2 29.3 QDPYSGR 621.98_694.3 HPCA_HUMAN LSLEEFIR LSLEEFIR_503.78_693.4 503.78 693.4 21.7 66 24.7 TFR2_HUMAN VGVISFAQK VGVISFAQK_474.78_693.4 474.78 693.4 14.8 62.2 23.4 DIRA2_HUMAN VAVFGAGGVG VAVFGAGGVGK_481.28_ 481.28 692.4 13.16 63.1 23.7 K 692.4 GRN_HUMAN ENATTDLLTK ENATTDLLTK_553.29_690.4 553.29 690.4 12.44 72.4 26.8 NECP2_HUMAN AAEWQLDQPS AAEWQLDQPSWSGR_ 815.88 689.3 17.32 106.2 38.4 WSGR 815.88_689.3 SAA4_HUMAN GPGGVWAAK GPGGVWAAK_421.73_688.4 421.73 688.4 11.21 55.4 24.1 AACT_HUMAN ITLLSALVET ITLLSALVETR_608.37_688.4 608.37 688.4 23.62 79.5 26.3 R TRIM9_HUMAN VLEGVIDR VLEGVIDR_450.76_688.4 450.76 688.4 12.51 59.1 22.3 CLC4M_HUMAN AAVGELPDQS AAVGELPDQSK_557.79_ 557.79 687.4 9.74 73 27 K 687.4 OLIG1_HUMAN IATLLLAR IATLLLAR_435.79_686.5 435.79 686.5 17.19 57.2 21.7 ELAV3_HUMAN GLNGQKPLGA GLNGQKPLGAAEPITVK_ 564.99 686.4 13.41 73.9 26.4 AEPITVK 564.99_686.4 PSMG1_HUMAN LDLITVEAFK LDLITVEAFKPILSTR_606.02 606.02 686.4 23.92 79.2 34.5 PILSTR _686.4 CADH5_HUMAN YEIVVEAR YEIVVEAR_489.8_686.4 489.8 686.4 14 64.2 27 KSYK_HUMAN LIATTAHEK LIATTAHEK_492.28_686.3 492.28 686.3 6.84 64.5 24.2 CBPN_HUMAN SIPQVSPVR SIPQVSPVR_491.8_685.5 491.8 685.5 11.6 64.4 27.1 CEL3A_HUMAN EGPEQVIPIN EGPEQVIPINSEELFVHPLW 868.45 685.4 24.05 113 50.8 SEELFVHPLW NR_868.45_685.4 NR CEL3B_HUMAN EGPEQVIPIN EGPEQVIPINSGDLFVHPLW 839.77 685.4 23.81 109.3 49.4 SGDLFVHPLW NR_839.77_685.4 NR KCC2A_HUMAN ITQYLDAGGI ITQYLDAGGIPR_652.35_ 652.35 685.4 14.93 85.2 31.2 PR 685.4 PCDBC_HUMAN ALDYEERPEL ALDYEERPELSFILTALDGG 882.79 685.3 25.09 114.9 42.6 SFILTALDGG SPPR_882.79_685.3 SPPR PCDBF_HUMAN LTLTAVDGGS LTLTAVDGGSPPR_428.57_ 428.57 685.3 13.73 56.3 28.4 PPR 685.3 PCDB6_HUMAN SLDYEALQSF SLDYEALQSFEFR_802.88_ 802.88 685.3 21.86 104.6 37.8 EFR 685.3 LGI1_HUMAN FQELNVQAPR FQELNVQAPR_601.32_684.4 601.32 684.4 13.58 78.6 32 CSF1R_HUMAN VIPGPPALTL VIPGPPALTLVPAELVR_ 581.36 684.4 24.37 76 24.2 VPAELVR 581.36_684.4 PTN5_HUMAN QLSHQSPE QLSHQSPE_463.22_684.3 463.22 684.3 6.3 60.8 22.9 PERL_HUMAN IHGFDLAAI IHGFDLAAINTQR_485.93_ 485.93 683.4 17.09 63.7 22.3 NTQR 683.4 IL12B_HUMAN DIIKPDPPK DIIKPDPPK_511.8_682.4 511.8 682.4 9.7 67 25 NPTX1_HUMAN LENLEQYSR LENLEQYSR_576.29_682.3 576.29 682.3 12.21 75.3 27.9 PCDB6_HUMAN DLDAGSFGK DLDAGSFGK_455.22_681.3 455.22 681.3 11.75 59.7 22.5 PTN5_HUMAN QLSHQSPE QLSHQSPE_463.22_681.3 463.22 681.3 6.3 60.8 22.9 ALBU_HUMAN AEFAEVSK AEFAEVSK_440.72_680.4 440.72 680.4 9.69 57.9 21.9 FINC_HUMAN SYTITGLQP SYTITGLQPGTDYK_772.39_ 772.39 680.3 15.02 100.6 36.5 GTDYK 680.3 FEZ1_HUMAN GLSLQSSR GLSLQSSR_424.24_677.4 424.24 677.4 9.94 55.7 21.2 IL1R2_HUMAN TISASLGSR TISASLGSR_446.25_677.4 446.25 677.4 10.01 58.6 22.1 CD72_HUMAN VLEVTNSSL VLEVTNSSLR_559.31_677.4 559.31 677.4 12.4 73.2 27.1 R CD3D_HUMAN NDQVYQPLR NDQVYQPLR_566.79_676.4 566.79 676.4 11.59 74.1 27.4 SCN2A_HUMAN ALIESNQTA ALIESNQTAR_551.8_676.3 551.8 676.3 8.92 72.2 29.8 R RCN1_HUMAN TFDQLTPDE TFDQLTPDESK_640.8_676.3 640.8 676.3 12.13 83.7 30.7 SK OLFL3_HUMAN DPLGQTEK DPLGQIEK_444.23_675.4 444.23 675.4 7.6 58.3 22 5HT2C_HUMAN VAATALSGR VAATALSGR_423.25_675.4 423.25 675.4 8.46 55.6 21.1 VTDB_HUMAN VPTADLEDV VPTADLEDVLPLAEDITNIL 789.43 675.4 25.27 102.8 34.8 LPLAEDITN SK_789.43_675.4 ILSK CB080_HUMAN FSDTQPK FSDTQPK_411.7_675.3 411.7 675.3 6.65 54.1 20.6 KCNV2_HUMAN FSDYYSK FSDYYSK_455.2_675.3 455.2 675.3 9.95 59.7 22.5 OPCM_HUMAN NTGVSVGQK NTGVSVGQK_445.24_674.4 445.24 674.4 6.78 58.4 22.1 KIRR2_HUMAN AEAGLSGLR AEAGLSGLR_437.24_673.4 437.24 673.4 11.57 57.4 21.7 A2MG_HUMAN LLIYAVLPT LLIYAVLPTGDVIGDSAK_ 615.68 673.4 23.29 80.4 25.9 GDVIGDSAK 615.68_673.4 CO9A2_HUMAN VGDPGVAGL VGDPGVAGLPGEK_598.32_ 598.32 671.4 12.25 78.2 37.8 PGEK 671.4 APC2_HUMAN APATASATL APATASATLLE_522.78_ 522.78 671.3 14.37 68.4 25.5 LE 671.3 KCNA1_HUMAN DFTGTVHR DFTGTVHR_466.73_670.4 466.73 670.4 8.37 61.2 29 CO1A2_HUMAN GPAGPSGPA GPAGPSGPAGK_448.24_ 448.24 670.4 6.21 58.8 25.2 GK 670.4 NRG3_HUMAN DLVGYSSTR DLVGYSSTR_499.25_670.3 499.25 670.3 10.78 65.4 24.5 NEUM_HUMAN EEEPEADQE EEEPEADQEHA_642.26_ 642.26 670.3 6.66 83.9 39.8 HA 670.3 BEST1_HUMAN WWNQYENLP WWNQYENLPWPDR_902.42 902.42 670.3 21.96 117.4 42.2 WPDR _670.3 TAU_HUMAN TPSLPTPPT TPSLPTPPTR_533.8_668.4 533.8 668.4 12.13 69.9 29 R YQ051_HUMAN SLADPPSSS SLADPPSSSQALHSPAR_ 574.29 668.3 10.78 75.1 26.8 QALHSPAR 574.29_668.3 SYUB_HUMAN EGVLYVGSK EGVLYVGSK_476.2_666.4 476.2 666.4 11.9 62.4 23.5 FETUA_HUMAN FSVVYAK FSVVYAK_407.23_666.4 407.23 666.4 12.73 53.5 20.4 DCBD2_HUMAN NNFLPPIIA NNFLPPIIAR_577.84_666.4 577.84 666.4 19.88 75.5 27.9 R PARK7_HUMAN VTTHPLAK VTTHPLAK_433.76_666.4 433.76 666.4 6.73 57 21.6 CADH3_HUMAN FTQDTFR FTQDTFR_457.72_666.3 457.72 666.3 10.91 60 22.6 ANGT_HUMAN ADSQAQLLL ADSQAQLLLSTVVGVFTAP 822.46 664.4 25.25 107.1 48.5 STVVGVFTA GLHLK_822.46_664.4 PGLHLK ICAM3_HUMAN IALETSLSK IALETSLSK_481.28_664.4 481.28 664.4 13.28 63.1 23.7 FGFR2_HUMAN EIEVLYIR EIEVLYIR_517.8_663.4 517.8 663.4 18.55 67.8 25.3 CAR14_HUMAN EQTLLQFQK EQTLLQFQK_567.81_663.4 567.81 663.4 15.65 74.2 27.5 PAK3_HUMAN LLQTSNITK LLQTSNITK_509.3_663.4 509.3 663.4 10.64 66.7 24.9 PLCB1_HUMAN NDSIPQEDF NDSIPQEDFTPEVYR_905.42 905.42 663.3 16.36 117.8 51.3 TPEVYR _663.3 MICA_HUMAN ASGFYPWNI ASGFYPWNITLSWR_849.43 849.43 662.4 25.08 110.6 36.9 TLSWR _662.4 MAMC2_HUMAN FTSQPGYIG FTSQPGYIGR_563.29_662.4 563.29 662.4 11.01 73.7 27.3 R PACN1_HUMAN AGDELTK AGDELTK_367.19_662.3 367.19 662.3 6.7 48.4 18.7 VGFR3_HUMAN SGVDLADSN SGVDLADSNQK_567.28_ 567.28 662.3 8.61 74.2 27.5 QK 662.3 AACT_HUMAN ADLSGITGA ADLSGITGAR_480.76_661.4 480.76 661.4 11.68 63 23.7 R A1AG2_HUMAN EHVAHLLFL EHVAHLLFLR_412.24_661.4 412.24 661.4 17.82 54.2 24.6 R SLIK1_HUMAN SLPVDVFAG SLPVDVFAGVSLSK_709.9_ 709.9 661.4 22.04 92.6 39.7 VSLSK 661.4 HPT_HUMAN VGYVSGWGR VGYVSGWGR_490.75_661.3 490.75 661.3 13.84 64.3 24.1 TRPV5_HUMAN AEGPGSQLQ AEGPGSQLQK_507.76_660.4 507.76 660.4 7.63 66.5 33.8 K ALS_HUMAN SLALGTFAH SLALGTFAHTPALASLGLSN 737.73 660.3 21.95 96.2 32.2 TPALASLGL NR_737.73_660.3 SNNR F13A_HUMAN LSIQSSPK LSIQSSPK_430.25_659.4 430.25 659.4 9.07 56.5 21.4 ITB2_HUMAN LGAILTPND LGAILTPNDGR_563.81_ 563.81 659.3 13.18 73.7 27.3 GR 659.3 LAT2_HUMAN SGGDYSYVK SGGDYSYVK_488.22_659.3 488.22 659.3 9.01 64 27 CNTN2_HUMAN VIASNILGT VIASNILGTGEPSGPSSK_ 857.45 659.3 14.84 111.6 43.2 GEPSGPSSK 857.45_659.3 MMP17_HUMAN VLDGELEVA VLDGELEVAPGYPQSTAR_ 951.48 659.3 15.62 123.7 47.4 PGYPQSTAR 951.48_659.3 APOD_HUMAN VLNQELR VLNQELR_436.25_659.3 436.25 659.3 9.77 57.3 21.7 PCDGF_HUMAN DLGFGVGDL DLGFGVGDLPTR_623.83_ 623.83 658.4 18.4 81.5 29.9 PTR 658.4 LAMB2_HUMAN SLADVDAIL SLADVDAILAR_572.32_ 572.32 658.4 19.32 74.8 27.7 AR 658.4 GELS_HUMAN TGAQELLR TGAQELLR_444.2_658.4 444.2 658.4 11.1 58.3 25 TFR2_HUMAN YGDVVLR YGDVVLR_411.23_658.4 411.23 658.4 11.84 54 23.6 FCGRN_HUMAN GDDTGVLLP GDDTGVLLPTPGEAQDADL 1006.49 658.3 16.76 130.8 46.8 TPGEAQDAD K_1006.49_658.3 LK CAD11_HUMAN DPDAANSPI DPDAANSPIR_528.26_657.4 528.26 657.4 8.63 69.1 28.7 R S15A2_HUMAN IEDIPANK IEDIPANK_450.25_657.4 450.25 657.4 8.5 59.1 22.3 FINC_HUMAN SSPVVIDAS S SPVVIDASTAIDAPSNLR_ 957 657.4 17.46 124.5 53.6 TAIDAPSNL 957_657.4 R KIRR2_HUMAN FLVETFPAP FLVETFPAPESR_696.86_ 696.86 656.3 17.78 90.9 33.2

ESR 656.3 GRIA2_HUMAN FAYLYDSDR FAYLYDSDR_575.26_655.3 575.26 655.3 14.83 75.2 27.8 MMP1_HUMAN SQNPVQPIG SQNPVQPIGPQTPK_745.9_ 745.9 654.3 10.67 97.2 32.3 PQTPK 654.3 VTNC_HUMAN NGSLFAFR NGSLFAFR_456.2_653.4 456.2 653.4 19.7 59.9 25.6 ITB5_HUMAN LGFGSFVDK LGFGSFVDK_485.26_652.3 485.26 652.3 17.93 63.6 23.9 GPC6_HUMAN SAPENFNTR SAPENFNTR_518.25_651.3 518.25 651.3 8.44 67.9 34.3 PTPRB_HUMAN TVVLQTDPL TVVLQTDPLPPAR_703.9_ 703.9 650.4 14.83 91.8 42.5 PPAR 650.4 NECP2_HUMAN VRPASTGGL VRPASTGGLSLLPPPPGGK_ 601.02 649.4 16.62 78.5 28.2 SLLPPPPGG 601.02_649.4 K CRLF1_HUMAN LAGLKPGTV LAGLKPGTVYFVQVR_ 549.99 648.4 18.54 71.9 25.6 YFVQVR 549.99_648.4 RGS4_HUMAN LGFLLQK LGFLLQK_409.76_648.4 409.76 648.4 18.02 53.9 20.5 VTNC_HUMAN FEDGVLDPD FEDGVLDPDYPR_711.83_ 711.83 647.3 16.02 92.8 42.8 YPR 647.3 APOA1_HUMAN LAEYHAK LAEYHAK_416.22_647.3 416.22 647.3 6.59 54.7 20.8 CALL3_HUMAN DGNGFVSAA DGNGFVSAAELR_618.3_ 618.3 646.4 15.13 80.8 29.7 ELR 646.4 TNR19_HUMAN VLLEQEK VLLEQEK_429.75_646.3 429.75 646.3 9.97 56.4 21.4 GPR88_HUMAN SVLPGVGDA SVLPGVGDAAAAAVAATA 907.16 645.4 22.31 118 40.8 AAAAVAATA VPAVSQAQLGTR_907.16_ VPAVSQAQL 645.4 GTR GPC5_HUMAN LLEQVNR LLEQVNR_436.25_645.3 436.25 645.3 9.5 57.3 21.7 ACHA4_HUMAN APPPHLPLS APPPHLPLSPALTR_489.62_ 489.62 644.4 16.02 64.2 28.5 PALTR 644.4 S12A5_HUMAN ETTAGHLAL ETTAGHLALLVTK_451.93_ 451.93 644.4 15.98 59.3 23.6 LVTK 644.4 HS3S5_HUMAN IHPEVDPSV IHPEVDPSVITK_445.58_ 445.58 644.4 12.75 58.5 20.3 ITK 644.4 RGS20_HUMAN IIYEDYISI IIYEDYISIL SPK_777.43_ 777.43 644.4 22.75 101.3 33.7 LSPK 644.4 FIBB_HUMAN SILENLR SILENLR_422.75_644.4 422.75 644.4 15.36 55.5 21.1 A1BG_HUMAN ATWSGAVLA ATWSGAVLAGR_544.8_ 544.8 643.3 15.9 71.3 32.5 GR 643.3 BEST1_HUMAN FLGLQSHDH FLGLQSHDHHPPR_514.26_ 514.26 643.3 11.25 67.3 32.8 HPPR 643.3 KALRN_HUMAN LTLSDFLIK LTLSDFLIKPIQR_515.31_ 515.31 641.4 23.26 67.5 32.8 PIQR 641.4 PARD3_HUMAN ESVSTASDQ ESVSTASDQPSHSLER_ 577.27 641.3 8.68 75.5 36 PSHSLER 577.27_641.3 COIA1_HUMAN ADDILASPP ADDILASPPR_527.78_640.4 527.78 640.4 12.5 69.1 22.7 R EDNRB_HUMAN SLAPAEVPK SLAPAEVPK_456.26_640.4 456.26 640.4 10.48 59.9 22.6 LIPE_HUMAN VVGHSIAR VVGHSIAR_419.75_640.4 419.75 640.4 6.61 55.1 27 SIX3_HUMAN DLYHILENH DLYHILENHK_427.89_640.3 427.89 640.3 16.71 56.2 25.4 K CATD_HUMAN LSPEDYTLK LSPEDYTLK_533.28_639.3 533.28 639.3 13.66 69.8 35 PDYN_HUMAN LSGSFLK LSGSFLK_376.22_638.4 376.22 638.4 13.45 49.5 19.1 TM9S1_HUMAN IIHTDVFR IIHTDVFR_334.19_637.3 334.19 637.3 12.94 44.1 17.6 ARSE_HUMAN VVHHDPPLL VVHHDPPLLFDLSR_548.97_ 548.97 637.3 19.49 71.8 25.5 LSFDR 637.3 PAQR6_HUMAN LPQLLQVHQ LPQLLQVHQVPR_476.62_ 476.62 636.4 16.86 62.5 27.9 VPR 636.4 IL1AP_HUMAN LYIEYGIQR LYIEYGIQR_577.81_636.3 577.81 636.3 15.79 75.5 27.9 HEP2_HUMAN IAIDLFK IAIDLFK_410.25_635.4 410.25 635.4 19.9 53.9 20.6 C1QT4_HUMAN VPGAYFFSF VPGAYFFSFTAGK_464.57_ 464.57 635.3 22.38 60.9 21.2 TAGK 635.3 LRP8_HUMAN LYWVDSK LYWVDSK_455.7_634.3 455.7 634.3 15.9 59.8 22.6 CADH3_HUMAN STGTISVIS STGTISVISSGLDR_696.87_ 696.87 634.3 16.02 90.9 33.2 SGLDR 634.3 AACT_HUMAN EIGELYLPK EIGELYLPK_531.3_633.5 531.3 633.5 18.4 69.5 25.9 PCDB7_HUMAN ILVLDVNDN ILVLDVNDNAPDFVR_ 850.45 633.3 20.44 110.7 36.9 APDFVR 850.45_633.3 ICAM3_HUMAN WEEELSR WEEELSR_474.72_633.3 474.72 633.3 11.56 62.2 26.4 PDYN_HUMAN FLPSISTK FLPSISTK_446.76_632.4 446.76 632.4 13.97 58.6 22.2 ODP2_HUMAN ILVAEGTR ILVAEGTR_429.76_632.3 429.76 632.3 9.62 56.4 21.4 CBPN_HUMAN IVQLIQDTR IVQLIQDTR_543.32_632.3 543.32 632.3 14.84 71.1 35.4 WNT8B_HUMAN TLGLLGTEG TLGLLGTEGR_508.79_632.3 508.79 632.3 15.38 66.6 24.9 R CSF1R_HUMAN ALTFELTLR ALTFELTLR_532.3_631.3 532.3 631.3 23.2 69.7 28.9 ITB5_HUMAN DDLDNIR DDLDNIR_430.71_630.4 430.71 630.4 10.48 56.6 21.5 MMP20_HUMAN EDVVSVVK EDVVSVVK_437.75_630.4 437.75 630.4 11.29 57.5 21.8 ATS1_HUMAN GAFYLLGEA GAFYLLGEAYFIQPLPAASE 1157.1 630.3 25.09 150.3 50.4 YFIQPLPAA R_1157.1_630.3 SER AT2A2_HUMAN WGSNELPAE WGSNELPAEEGK_658.81_ 658.81 630.3 12.28 86 34.5 EGK 630.3 PRIO_HUMAN ESQAYYQR ESQAYYQR_522.74_629.3 522.74 629.3 7.39 68.4 28.5 ACHA4_HUMAN SPSDQLPPQ SPSDQLPPQQPLEAEK_ 882.44 628.3 11.76 114.8 41.3 LQPEAEK 882.44_628.3 KLKB1_HUMAN TGAVSGHSL TGAVSGHSLK_478.76_628.3 478.76 628.3 6.61 62.8 29.6 K ACHB_HUMAN AVALLPELR AVALLPELR_491.31_627.4 491.31 627.4 19.41 64.4 24.1 IPSP_HUMAN GFQQLLQEL GFQQLLQELNQPR_524.28_ 524.28 627.4 23.15 68.6 24.3 NQPR 627.4 ARHG7_HUMAN SYYNVVLQN SYYNVVLQNILETENEYSK_ 1153.56 627.3 24.95 149.8 50.3 ILETENEYS 1153.56_627.3 K F123A_HUMAN DPAGEPAVG DPAGEPAVGEEVPAPADR_ 888.92 626.3 11.94 115.7 44.6 EEVPAPADR 888.92_626.3 NPT4_HUMAN WGPPQER WGPPQER_435.22_626.3 435.22 626.3 10.39 57.1 24.6 KCNA3_HUMAN SPPPPSAR SPPPPSAR_404.72_624.3 404.72 624.3 6.02 53.2 20.3 FGFR3_HUMAN VGPDGTPYV VGPDGTPYVTVLK_673.37_ 673.37 624.3 15.91 87.9 32.1 TVLK 624.3 MYP2_HUMAN NTEISFK NTEISFK_419.72_623.3 419.72 623.3 11.93 55.1 21 SEPT3_HUMAN STLVNTLFK STLVNTLFK_511.8_622.4 511.8 622.4 19.5 67 25 PICAL_HUMAN ITAAQHSVT ITAAQHSVTGSAVSK_ 486.26 622.3 7.72 63.7 22.3 GSAVSK 486.26_622.3 CETP_HUMAN VIQTAFQR VIQTAFQR_481.77_622.3 481.77 622.3 10.75 63.1 23.7 CK041_HUMAN DVAQDGSTI DVAQDGSTIK_517.26_620.3 517.26 620.3 7.48 67.7 25.3 K FIBG_HUMAN IHLISTQSA IHLISTQSAIPYALR_561.66_ 561.66 619.4 18.84 73.5 26.2 IPYALR 619.4 SGCZ_HUMAN LEGISEFLL LEGISEFLLPLYVK_810.97_ 810.97 619.4 25.2 105.6 47.2 PLYVK 619.4 ACHA2_HUMAN LSPSYHWLE LSPSYHWLESNVDAEER_ 677.99 619.3 17.4 88.5 32.1 SNVDAEER 677.99_619.3 CD72_HUMAN NWQESQK NWQESQK_460.22_619.3 460.22 619.3 7.02 60.4 22.7 KCNQ1_HUMAN GQVFATSAI GQVFATSAIR_525.29_618.4 525.29 618.4 13.2 68.8 25.6 R TMPS2_HUMAN TSEVLNAAK TSEVLNAAK_466.76_615.4 466.76 615.4 8.64 61.2 26 DCBD1_HUMAN ASSSWQSVN ASSSWQSVNESGDQVHWS 834.05 615.3 14.41 108.6 49.1 ESGDQVHWS PGQAR_834.05_615.3 PGQAR CAMKV_HUMAN LENLVYYNR LENLVYYNR_592.31_615.3 592.31 615.3 14.32 77.4 28.6 UNC5C_HUMAN NEDIIDPVE NEDIIDPVEDR_657.81_615.3 657.81 615.3 13.99 85.9 40.4 DR MMP9_HUMAN SLGPALLLL SLGPALLLLQK_576.87_ 576.87 614.4 22.28 75.4 30.9 QK 614.4 KCNA5_HUMAN EEQGTQSQG EEQGTQSQGPGLDR_751.35 751.35 614.3 7.7 97.9 38.6 GPLDR _614.3 DCBD1_HUMAN HEYALPLAP HEYALPLAPPEPEYATPIVE 798.08 614.3 19.34 104 38.3 PEPEYATPI R_798.08_614.3 VER ARSE_HUMAN LAGGEVPQD LAGGEVPQDR_521.27_614.3 521.27 614.3 7.99 68.2 25.4 R LCN8_HUMAN FAFPGHR FAFPGHR_416.22_613.3 416.22 613.3 12.58 54.7 23.8 HPT_HUMAN FTDHLK FTDHLK_380.7_613.3 380.7 613.3 8.41 50.1 22.3 CO1A2_HUMAN GPAGPSGPA GPAGPSGPAGK_448.24_ 448.24 613.3 6.21 58.8 25.2 GK 613.3 RGS11_HUMAN WGFSFR WGFSFR_400.2_613.3 400.2 613.3 20.25 52.6 23.1 ACHB2_HUMAN IVPPTSLDV IVPPTSLDVPLVGK_717.93_ 717.93 612.4 19.09 93.6 34.1 PLVGK 612.4 SYNPR_HUMAN ETGWHSSGQ ETGWHSSGQR_572.76_611.3 572.76 611.3 7.62 74.9 36.7 R GPC6_HUMAN GFSLADIPY GFSLADIPYQEIAGEHLR_ 672.68 611.3 22.21 87.8 34.9 QEIAGEHLR 672.68_611.3 SO1A2_HUMAN IYDSTTFR IYDSTTFR_501.75_611.3 501.75 611.3 11.46 65.7 33.6 FIBA_HUMAN TVIGPDGHK TVIGPDGHK_462.25_610.3 462.25 610.3 6.93 60.6 25.8 CALL3_HUMAN EAFSLFDK EAFSLFDK_478.74_609.3 478.74 609.3 18.7 62.8 23.6 FGL1_HUMAN IDLADFEK IDLADFEK_475.75_609.3 475.75 609.3 17.24 62.4 23.4 MMP20_HUMAN LAQAYLDK LAQAYLDK_461.26_609.3 461.26 609.3 10.6 60.5 22.8 SCRT1_HUMAN SDLGAPLHD SDLGAPLHDK_526.77_609.3 526.77 609.3 9.54 69 28.7 K DCBD2_HUMAN YSSSEVNHL YSSSEVNHLSPR_459.23_ 459.23 609.3 9.61 60.2 27 SPR 609.3 PIN1_HUMAN EEALELING EEALELINGYIQK_507.27_ 507.27 608.3 20.61 66.4 23.4 YIQK 608.3 NEUG_HUMAN IQASFR IQASFR_361.2_608.3 361.2 608.3 9.77 47.6 21.4 LAMB2_HUMAN IQGTLQPHA IQGTLQPHAR_374.21_608.3 374.21 608.3 7.74 49.3 22.6

R CFAH_HUMAN NGFYPATR NGFYPATR_463.23_607.3 463.23 607.3 11.09 60.8 22.9 KNG1_HUMAN YFIDFVAR YFIDFVAR_515.8_607.3 515.8 607.3 22.5 67.5 28.2 FA20A_HUMAN HFPTISADY HFPTISADYSQDEK_546.59_ 546.59 606.3 13.78 71.5 25.4 SQDEK 606.3 CB080_HUMAN NATSISAK NATSISAK_396.22_606.3 396.22 606.3 6.49 52.1 19.9 ACHA2_HUMAN SEDADSSVK SEDADSSVK_469.21_606.3 469.21 606.3 6 61.5 26.1 ZIC1_HUMAN HHSAGDVAE HHSAGDVAER_360.17_ 360.17 605.2 5.9 47.5 18.9 R 605.2 GBRB2_HUMAN GDDNAVTGV GDDNAVTGVTK_538.76_ 538.76 604.4 7.83 70.5 26.2 TK 604.4 TR11B_HUMAN NGDQDTLK NGDQDTLK_445.71_604.3 445.71 604.3 6.5 58.5 25.1 NCKX2_HUMAN TLLDLNDK TLLDLNDK_466.26_604.3 466.26 604.3 15.03 61.1 23 TERA_HUMAN GGNIGDGGG GGNIGDGGGAADR_558.76_ 558.76 603.3 6.49 73.1 36.1 AADR 603.3 ALS_HUMAN SLALGTFAH SLALGTFAHTPALASLGLSN 737.73 603.3 21.95 96.2 32.2 TPALNASLG NR_737.73_603.3 LSNR KIRR2_HUMAN AEAGLSGLR AEAGLSGLR_437.24_602.4 437.24 602.4 11.57 57.4 21.7 I12R1_HUMAN AGVAYTVQV AGVAYTVQVR_532.3_602.4 532.3 602.4 12.09 69.7 25.9 R ACHA5_HUMAN GLSEPSSIA GLSEPSSIAK_494.77_602.4 494.77 602.4 10.19 64.8 24.3 K CACB2_HUMAN GVGVVDQAL GVGVVDQALR_507.3_602.3 507.3 602.3 11.8 66.4 27.8 R APOA4_HUMAN LEPYADQLR LEPYADQLR_552.79_602.3 552.79 602.3 12.59 72.3 32.8 ITIH3_HUMAN NHQLIVTR NHQLIVTR_490.79_601.4 490.79 601.4 9.24 64.3 33.1 TLR7_HUMAN NLETLDLSH NLETLDLSHNQLTTVPER_ 694.03 601.3 16.88 90.5 32.9 NQLTTVPER 694.03_601.3 BDNF_HUMAN THGTLESVN THGTLESVNGPK_413.88_ 413.88 601.3 8.23 54.4 18.7 GPK 601.3 MTOR_HUMAN TLDQSPELR TLDQSPELR_529.78_601.3 529.78 601.3 10.35 69.3 28.8 RRAGC_HUMAN VDGLSDDHK VDGLSDDHK_493.23_601.3 493.23 601.3 6.78 64.6 27.2 NOE3_HUMAN ITGPVTVK ITGPVTVK_407.76_600.4 407.76 600.4 10.07 53.6 20.4 GFRA2_HUMAN LASIFSGTG LASIFSGTGADPVVSAK_ 810.44 600.4 17.97 105.5 47.2 ADPVVSAK 810.44_600.4 TNF6B_HUMAN LLQALR LLQALR_357.24_600.4 357.24 600.4 12.48 47.1 21.2 SO1B3_HUMAN LSLVGIAK LSLVGIAK_400.77_600.4 400.77 600.4 15.8 52.7 20.1 CSF1_HUMAN ISSLRPQGL ISSLRPQGLSNPSTLSAQPQL 813.11 600.3 15.09 105.9 39 SNPSTLSAQ SR_813.11_600.3 PQLSR CO8A_HUMAN SLLQPNK SLLQPNK_400.24_599.4 400.24 599.4 10.77 52.6 20.1 PTPRO_HUMAN IANLLPAWY IANLLPAWYYNFR_547.62_ 547.62 599.3 24.56 71.6 22.5 YNFR 599.3 PON1_HUMAN SFNPNSPGK SFNPNSPGK_474.23_599.3 474.23 599.3 7.72 62.2 23.4 CNTN2_HUMAN VIASNILGT VIASNILGTGEPSGPSSK_ 857.45 598.4 14.84 111.6 40.2 GGEPSPSSK 857.45_598.4 MMP17_HUMAN ESVSPTAQP ESVSPTAQPEEPPLLPEPPD 800.4 598.3 16.38 104.3 41.4 LLEEPPPEP NR_800.4_598.3 PDNR KAIN_HUMAN VGSALFLSH VGSALFLSHNLK_429.2_ 429.2 598.3 17.1 56.4 25.4 NLK 598.3 ANGP1_HUMAN LEIQLLENS LEIQLLENSLSTYK_825.95_ 825.95 597.4 22.62 107.5 38.8 LSTYK 597.4 LRP8_HUMAN EPEDPAPAL EPEDPAPALK_533.77_596.4 533.77 596.4 10.24 69.9 26 K ANGP1_HUMAN DAPHVEPDF DAPHVEPDFSSQK_486.23_ 486.23 596.3 11 63.7 25.3 SSQK 596.3 SIX3_HUMAN AVVAFHTGN AVVAFHTGNFR_406.88_ 406.88 594.3 13.85 53.5 27.3 FR 594.3 PEDF_HUMAN LQSLFDSPD LQSLFDSPDFSK_692.34_ 692.34 593.3 18.9 90.3 42 FSK 593.3 APOE_HUMAN LQAEAFQAR LQAEAFQAR_517.28_592.3 517.28 592.3 11.18 67.7 31.3 SCG3_HUMAN ELSAERPLN ELSAERPLNEQIAEAEEDK_ 724.35 591.3 15.56 94.4 43.5 AEQIEAEED 724.35_591.3 K AMNLS_HUMAN ILDTFLGLP ILDTFLGLPQYHGLQVAVS 733.74 590.3 22.88 95.7 32 QYHKGLQVA K_733.74_590.3 VS LRTM2_HUMAN LSALPSWAF LSALPSWAFANLSSLQR_ 621 590.3 24.55 81.1 29.2 SANLSLQR 621_590.3 SYN2_HUMAN VLLVVDEPH VLLVVDEPHADWAK_ 531.29 590.3 17.62 69.5 33.6 ADWAK 531.29_590.3 PARK7_HUMAN DGLILTSR DGLILTSR_437.75_589.4 437.75 589.4 13.64 57.5 21.8 GRN_HUMAN ENATTDLLT ENATTDLLTK_553.29_589.4 553.29 589.4 12.44 72.4 26.8 K ARP21_HUMAN TASFGGITV TASFGGITVLTR_611.84_ 611.84 589.4 18.32 79.9 29.4 LTR 589.4 KALRN_HUMAN DFFLAELEK DFFLAELEK_556.29_589.3 556.29 589.3 22.36 72.8 27 GBRA2_HUMAN LNQYDLLGQ LNQYDLLGQSIGK_724.89_ 724.89 589.3 17.87 94.5 34.4 SIGK 589.3 SYUA_HUMAN EGVVAAAEK EGVVAAAEK_437.24_588.3 437.24 588.3 7.7 57.4 24.7 CB080_HUMAN FSDTQPK FSDTQPK_411.7_588.3 411.7 588.3 6.65 54.1 20.6 APOE_HUMAN LGPLVEQGR LGPLVEQGR_484.78_588.3 484.78 588.3 11.76 63.5 29.8 CLUS_HUMAN SGSGLVGR SGSGLVGR_366.7_588.3 366.7 588.3 7.52 48.3 21.6 MPDZ_HUMAN SLGISIVGG SLGISIVGGR_479.79_588.3 479.79 588.3 15.92 62.9 23.6 R OTOAN_HUMAN THSWQDAPA THSWQDAPASAGPTR_ 527.92 588.3 9.56 69.1 33.5 SAGPTR 527.92_588.3 PDIA2_HUMAN EDGILVLSR EDGILVLSR_501.29_587.4 501.29 587.4 16.44 65.7 24.6 ACE2_HUMAN SEPWTLALE SEPWTLALENVVGAK_ 538.62 587.4 23.88 70.5 25 NVVGAK 538.62_587.4 FGFR3_HUMAN DGTGLVPSE DGTGLVPSER_515.76_587.3 515.76 587.3 10.4 67.5 25.2 R VTDB_HUMAN VLEPTLK VLEPTLK_400.25_587.3 400.25 587.3 11.66 52.6 20.1 AMFR2_HUMAN VPLDLSPR VPLDLSPR_448.76_587.3 448.76 587.3 13.3 58.9 25.2 OPCM_HUMAN WSIDPR WSIDPR_387.2_587.3 387.2 587.3 13.74 50.9 19.5 MMP24_HUMAN ADEAEAPFA ADEAEAPFAGQNWLK_ 823.89 587.2 19.35 107.3 35.8 GQNWLK 823.89_587.2 GNAQ_HUMAN AHAQLVR AHAQLVR_397.74_586.4 397.74 586.4 6.97 52.3 23 CAD11_HUMAN DPDAANSPI DPDAANSPIR_528.26 586.3 528.26 586.3 8.63 69.1 28.7 R CRUM1_HUMAN GDVIYIGGL GDVIYIGGLPDK_623.84_ 623.84 586.3 17.2 81.5 29.9 PDK 586.3 CACB2_HUMAN ATHPPSSSL ATHPPSSSLPNPLLSR_ 558.64 585.4 15.74 73.1 29 PPNLLSR 558.64_585.4 SIA8C_HUMAN YASPGAPR YASPGAPR_409.71_584.3 409.71 584.3 7.03 53.9 20.5 ACHB_HUMAN VPETSLSVP VPETSLSVPIIIK_698.43_ 698.43 583.4 19.99 91.1 42.2 IIIK 583.4 ACHB2_HUMAN EGAGALFFR EGAGALFFR_484.25_582.3 484.25 582.3 18.49 63.5 23.8 FIBG_HUMAN LTIGEGQQH LTIGEGQQHHLGGAK_ 515.94 582.3 9.73 67.6 32.9 GHLGAK 515.94_582.3 IL12B_HUMAN YENYTSSFF YENYTSSFFIR_476.23_582.3 476.23 582.3 19.63 62.4 18.8 IR CFAH_HUMAN NDFTWFK NDFTWFK_479.23_581.3 479.23 581.3 19.79 62.8 23.6 TFR2_HUMAN VGVISFAQK VGVISFAQK_474.78_580.3 474.78 580.3 14.8 62.2 23.4 FETUA_HUMAN FSVVYAK FSVVYAK_407.23_579.4 407.23 579.4 12.73 53.5 20.4 GT253_HUMAN NLGVDLLPG NLGVDLLPGYQDPYSGR_ 621.98 579.3 20.39 81.2 38.3 YQDPYSGR 621.98_579.3 TBB2B_HUMAN YLTVAAIFR YLTVAAIFR_527.31_577.3 527.31 577.3 22.63 69 28.7 CO3_HUMAN LSINTHPSQ LSINTHPSQKPLSITVR_ 631.03 575.4 14.39 82.4 35.7 KPLSITVR 631.03_575.4 PCDAA_HUMAN ALDETDSPR ALDETDSPR_502.24_575.3 502.24 575.3 7.31 65.8 27.6 GOPC_HUMAN EAQLEAEVK EAQLEAEVK)508.77_575.3 508.77 575.3 10.15 66.6 24.9 TM9S1_HUMAN SLSLGEVLD SLSLGEVLDGDR_630.83_ 630.83 575.3 19.62 82.4 30.3 GDR 575.3 RCN1_HUMAN TFDQLTPDE TFDQLTPDESK_640.8_575.3 640.8 575.3 12.13 83.7 30.7 SK AACT_HUMAN ADLSGITGA ADLSGITGAR_480.76_574.3 480.76 574.3 11.68 63 23.7 R ANGT_HUMAN DPTFIPAPI DPTFIPAPIQAK_649.36_ 649.36 574.3 16.67 84.8 28.1 QAK 574.3 GP2_HUMAN FVGEGGVR FVGEGGVR_410.72_574.3 410.72 574.3 9.22 54 20.6 XLRS1_HUMAN VISGILTQG VISGILTQGR_522.31_574.3 522.31 574.3 14.23 68.4 25.5 R C04A4_HUMAN ADLQFSSAP ADLQFSSAPAPDTLK_780.9 780.9 573.3 15.27 101.7 45.9 APDTLK _573.3 EAA2_HUMAN DLEVVAR DLEVVAR_401.23_573.3 401.23 573.3 10.94 52.8 20.2 ITB2_HUMAN VFLDHNALP VFLDHNALPDTLK_494.94_ 494.94 573.3 16.62 64.8 22.8 DTLK 573.3 EAA2_HUMAN VTLAANGK VTLAANGK_387.23_573.3 387.23 573.3 7.55 51 19.5 CADH5_HUMAN YEIVVEAR YEIVVEAR_489.77_573.3 489.77 573.3 13.14 64.2 24 S12A6_HUMAN LNEVIVNK LNEVIVNK_464.78_572.4 464.78 572.4 10.4 61 23 WNK4_HUMAN AAEDSARPE AAEDSARPELPDSAVGPGS 661.32 572.3 11.97 86.3 28.3 LPDSAVGPG R_661.32_572.3 SR LGMN_HUMAN DYTGEDVTP DYTGEDVTPQNFLAVLR_ 646.66 571.4 24.14 84.4 27.5 QNFLAVLR 646.66_571.4 UNC5C_HUMAN DFESDIIDS DFESDIIDSSALNGGFQPVNI 789.39 570.4 22.67 102.8 37.8 LSANGGFQP K_789.39_570.4 VNIK KNG1_HUMAN DFVQPPTK DFVQPPTK_466.25_570.3 466.25 570.3 10.45 61.1 23 BTNL8_HUMAN VQIGDTFFE VQIGDTFFEPISWHLATK_ 697.03 569.3 24.32 90.9 42.1

PISWHLATK 697.03_569.3 CO4A_HUMAN GSFEFPVGD GSFEFPVGDAVSK_670.33_ 670.33 568.2 17.53 87.5 29 AVSK 568.2 PCDB6_HUMAN DLDAGSFGK DLDAGSFGK_455.22_566.3 455.22 566.3 11.75 59.7 22.5 SREC_HUMAN HFGSFQK HFGSFQK_425.71_566.3 425.71 566.3 9.6 55.9 21.2 EMIL2_HUMAN LDNEFDR LDNEFDR_454.71_566.3 454.71 566.3 10.18 59.7 25.5 SEMG1_HUMAN LPSEFSQFP LPSEFSQFPHGQK_501.25_ 501.25 566.3 14.66 65.7 26.1 HGQK 566.3 NPT4_HUMAN WGPPQER WGPPQER_435.22_566.3 435.22 566.3 10.39 57.1 27.6 APLP1_HUMAN HYQHVAAVD HYQHVAAVDPEK_465.23_ 465.23 566.2 7.77 61 24.3 PEK 566.2 CLUS_HUMAN ASSIIDELF ASSIIDELFQDR_697.35_ 697.35 565.3 23.8 91 30.2 QDR 565.3 5HT2C_HUMAN ELNVNIYR ELNVNIYR_510.78_565.3 510.78 565.3 14.34 66.9 25 HEP2_HUMAN FAFNLYR FAFNLYR_465.75_565.3 465.75 565.3 19.02 61.1 23 EMIL3_HUMAN LQHTVGHFD LQHTVGHFDQR_446.56_ 446.56 565.3 8.57 58.6 26.3 QR 565.3 PARK7_HUMAN VTTHPLAK VTTHPLAK_433.76_565.3 433.76 565.3 6.73 57 24.6 T151A_HUMAN LFGASSPPP LFGASSPPPGAVPSGPPLSR_ 946.01 563.3 16.44 123 44.1 GAVPSGPPL 946.01_563.3 SR CALL3_HUMAN ELGTVMR ELGTVMR_403.2_563.2 403.2 563.2 9.7 53 20.2 OLFL3_HUMAN DPLGQTEK DPLGQ IEK_444.23_562.3 444.23 562.3 7.6 58.3 25 PVRL1_HUMAN ITQVTWQK ITQVTWQK_502.28_562.3 502.28 562.3 11.81 65.8 27.6 HPT_HUMAN VGYVSGWGR VGYVSGWGR_490.75_562.3 490.75 562.3 13.84 64.3 24.1 RUN3A_HUMAN FTQSYDYLT SYDYLTDEEER_898.39_ 898.39 562.2 15.16 116.9 51 DEEER 562.2 PCD18_HUMAN LLGEGFSDL LLGEGFSDLFLTDGR_820.42 820.42 561.3 24.6 106.8 38.6 FLTDGR 561.3 CNTN1_HUMAN AHSDGGDGV AHSDGGDGVVSQVK_ 452.56 560.3 8.14 59.4 20.6 VSQVK 452.56_560.3 GALR3_HUMAN EGPVHGGEA EGPVHGGEAAR_540.27_ 540.27 560.3 6.1 70.7 32.3 AR 560.3 ITIH1_HUMAN FAHYVVTSQ FAHYVVTSQVVNTANEAR_ 669.34 560.3 14.4 87.3 28.7 NVVTANEAR 669.34_560.3 STH_HUMAN NGAEQGR NGAEQGR_366.18_560.3 366.18 560.3 5.65 48.2 21.6 CO3_HUMAN IHWESASLL IHWESASLLR_404.56_559.4 404.56 559.4 17.03 53.2 21.2 R PICAL_HUMAN VAEQVGIDR VAEQVGIDR_493.77_559.3 493.77 559.3 9.18 64.7 30.2 TRIM9_HUMAN VLEGVIDR VLEGVIDR_450.76_559.3 450.76 559.3 12.51 59.1 25.3 AT2A2_HUMAN EFDELNPSA EFDELNPSAQR_653.31_ 653.31 558.3 12.74 85.3 31.2 QR 558.3 HPCA_HUMAN FAEHVFR FAEHVFR_453.24_558.3 453.24 558.3 11.78 59.5 25.4 KSYK_HUMAN YLEESNFVH YLEESNFVHR_431.88_558.3 431.88 558.3 12.59 56.7 25.6 R DDR2_HUMAN YLSSLNFVH YLSSLNFVHR_412.56_558.3 412.56 558.3 16.07 54.2 24.6 R CBPN_HUMAN SIPQVSPVR SIPQVSPVR_491.8_557.4 491.8 557.4 11.6 64.4 27.1 ACHA4_HUMAN APPPHLPLS APPPHLPLSPALTR_489.62_ 489.62 557.3 16.02 64.2 25.5 PALTR 557.3 RGS11_HUMAN WGFSFR WGFSFR_400.2_556.3 400.2 556.3 20.25 52.6 20.1 NETO1_HUMAN DGPFGFSPI DGPFGFSPIIGR_631.83_ 631.83 555.4 21.4 82.5 33.3 IGR 555.4 MMP16_HUMAN LPNDDLQGI LPNDDLQGIQK_620.83_ 620.83 555.2 12.2 81.1 38.8 QK 555.2 APOA4_HUMAN LVPFATELH LVPFATELHER_437.9_554.3 437.9 554.3 17.2 57.5 25.9 ER SYUA_HUMAN EGVLYVGSK EGVLYVGSK_476.26_553.3 476.26 553.3 12.17 62.4 23.5 E41L3_HUMAN IRPGEFEQF IRPGEFEQFESTIGFK_628.99 628.99 553.3 20.89 82.1 38.6 ESTIGFK 553.3 SCRT1_HUMAN LDAFSSADL LDAFSSADLESAYGR_ 801.38 553.3 17.31 104.4 40.8 ESAYGR 801.38_553.3 NPTX1_HUMAN LENLEQYSR LENLEQYSR_576.29_553.3 576.29 553.3 12.21 75.3 27.9 GPR88_HUMAN SVLPGVGDA SVLPGVGDAAAAAVAATA 907.16 553.3 22.31 118 43.8 AAAAVAATA VPAVSQAQLGTR_907.16_ SVPAVQAQL 553.3 GTR PDYN_HUMAN LSGSFLK LSGSFLK_376.22_551.3 376.22 551.3 13.45 49.5 19.1 PRIO_HUMAN QHTVTTTTK QHTVTTTTK_508.77_551.3 508.77 551.3 5.87 66.6 33.9 A1AG1_HUMAN WFYIASAFR WFYIASAFR_580.8_551.3 580.8 551.3 23.48 75.9 28.1 COIA1_HUMAN AVGLAGTFR AVGLAGTFR_446.2_551.2 446.2 551.2 14.6 58.6 22.1 PTPRT_HUMAN VTLIETEPL VTLIETEPLAEYVIR_582.66_ 582.66 550.3 23 76.2 27.3 AEYVIR 550.3 PCDGF_HUMAN YGPQFTLQH YGPQFTLQHVPDYR_574.29 574.29 550.3 16.79 75.1 35.8 VPDYR _550.3 CO6_HUMAN ALNHLPLEY ALNHLPLEYNSALYSR_ 930.98 549.3 17.62 121.1 46.5 NSALYSR 930.98_549.3 AT2B3_HUMAN NVYDSISK NVYDSISK_463.24_549.3 463.24 549.3 10.5 60.8 28.9 CATD_HUMAN YSQAVPAVT YSQAVPAVTEGPIPEVLK_ 949.52 549.3 19.28 123.5 41.3 EGPIPEVLK 949.52_549.3 A1AG2_HUMAN EHVAHLLFL EHVAHLLFLR_412.24_548.4 412.24 548.4 17.82 54.2 24.6 R XLRS1_HUMAN VFYGNSDR VFYGNSDR_479.23_548.2 479.23 548.2 8.8 62.8 26.6 SAA4_HUMAN AEEWGR AEEWGR_374.17_547.3 374.17 547.3 8.8 49.3 19 NPT4_HUMAN EYIISSLK EYIISSLK_476.77_547.3 476.77 547.3 15.53 62.5 23.5 KCNQ1_HUMAN GQVFATSAI GQVFATSAIR_525.29_547.3 525.29 547.3 13.2 68.8 25.6 R NMDZ1_HUMAN IPVLGLTTR IPVLGLTTR_485.31_547.3 485.31 547.3 17.9 63.6 32.9 GP113_HUMAN NLLIATDK NLLIATDK_444.26_547.3 444.26 547.3 13.39 58.3 22 F13A_HUMAN LSIQSSPK LSIQSSPK_430.25_546.3 430.25 546.3 9.07 56.5 21.4 CPLX2_HUMAN QALGGATK QALGGATK_373.21_546.3 373.21 546.3 6.4 49.1 18.9 APOA2_HUMAN SPELQAEAK SPELQAEAK_486.75_546.3 486.75 546.3 7.93 63.8 26.9 CNTN2_HUMAN VISDTEADI VISDTEADIGSNLR_745.38_ 745.38 546.3 13.74 97.2 35.3 GSNLR 546.3 F123A_HUMAN APAAPDPAS APAAPDPASVDPPSDPSAD 966.95 545.3 11.35 125.7 54 VDPPSDPSA R_966.95_545.3 DR S12A5_HUMAN DGIVPFLQV DGIVPFLQVFGHGK_505.28_ 505.28 545.3 24.51 66.2 29.3 FGHGK 545.3 TR11B_HUMAN HIGHANLTF HIGHANLTFEQLR_512.61_ 512.61 545.3 14.55 67.1 26.7 EQLR 545.3 KLK3_HUMAN HSQPWQVLV HSQPWQVLVASR_469.92_ 469.92 545.3 17.51 61.6 21.5 ASR 545.3 IGF1R_HUMAN TTINNEYNY TTINNEYNYR_644.3_544.3 644.3 544.3 9.54 84.1 39.8 R KNG1_HUMAN TVGSDTFYS TVGSDTFYSFK_626.3_544.3 626.3 544.3 16.46 81.8 39.1 FK CAMKV_HUMAN YDLGQVIK YDLGQVIK_468.26_544.3 468.26 544.3 15.27 61.4 23.1 PTPRT_HUMAN AVGSLDPSA AVGSLDPSADLSSQR_ 751.88 543.3 13.34 98 35.6 DLSSQR 751.88_543.3 RGS11_HUMAN ELLEDPVGR ELLEDPVGR_514.27_543.3 514.27 543.3 13.83 67.3 25.1 TRFR_HUMAN ILFLNPIPS ILFLNPIPSDPK_677.39_543.3 677.39 543.3 21.23 88.4 41.3 DPK APOOL_HUMAN LGSSSEIEV LGSSSEIEVPAK_608.82_ 608.82 543.3 11.43 79.5 29.3 PAK 543.3 GABR2_HUMAN TVPSDNAVN TVPSDNAVNPAILK_719.9_ 719.9 541.4 14.05 93.9 40.2 PAILK 541.4 CERU_HUMAN GAYPLSIEP GAYPLSIEPIGVR_686.39_ 686.39 541.3 19.06 89.5 32.7 IGVR 541.3 PCDA5_HUMAN LLENAPSGT LLENAPSGTLVIK_677.9_ 677.9 541.3 15.78 88.4 29.3 LVIK 541.3 KLKB1_HUMAN TGAVSGHSL TGAVSGHSLK_478.76_541.3 478.76 541.3 6.61 62.8 26.6 K BCAS1_HUMAN DPTLLPPET DPTLLPPETGGAGGEAPSKP 673.68 540.3 12.9 87.9 28.9 GGAGGEAPS K_673.68_540.3 KPK FA20A_HUMAN HNAEIAAFH HNAEIAAFHLDR_465.24_ 465.24 540.3 14.27 61 24.3 LDR 540.3 VTNC_HUMAN NGSLFAFR NGSLFAFR_456.2_540.3 456.2 540.3 19.7 59.9 25.6 CO2_HUMAN TAVDHIR TAVDHIR_406.22_540.3 406.22 540.3 7.01 53.4 26.4 ARTN_HUMAN ALGLGHR ALGLGHR_362.22_539.3 362.22 539.3 8.67 47.7 21.4 CADH3_HUMAN FTQDTFR FTQDTFR_457.72_538.3 457.72 538.3 10.91 60 25.6 GBRA2_HUMAN DPVLSTISK DPVLSTISK_480.27_535.3 480.27 535.3 12.44 63 26.6 ICAM3_HUMAN IALETSLSK IALETSLSK_481.28_535.3 481.28 535.3 13.28 63.1 29.7 KCNA3_HUMAN SNSTLSK SNSTLSK_368.7_535.3 368.7 535.3 6 48.6 18.7 ANT3_HUMAN ANRPFLVFI ANRPFLVFIR_411.58_534.3 411.58 534.3 20.79 54.1 21.5 R SYNPR_HUMAN ETGWHSSGQ ETGWHSSGQR_572.76_534.3 572.76 534.3 7.62 74.9 27.7 R APOOL_HUMAN HSVPLPTEL HSVPLPTELSSEAK_498.93_ 498.93 534.3 13.72 65.4 23 SSEAK 534.3 SLIT1_HUMAN QIPLQDVAF QIPLQDVAFPDFR_773.41_ 773.41 534.3 22.44 100.8 36.5 PDFR 534.3 ALS_HUMAN SFEGLGQLE SFEGLGQLEVLTLDHNQLQ 833.1 534.3 23.16 108.5 37 VLTLDHNQL EVK_833.1_534.3 QEVK ALBU_HUMAN AEFAEVSK AEFAEVSK_440.72_533.3 440.72 533.3 9.69 57.9 24.9 KLK3_HUMAN LSEPAELT LSEPAELTDAVK_636.84_ 636.84 533.3 14.48 83.2 33.5 DAVK 533.3 PEDF_HUMAN LSYEGEVTK LSYEGEVTK_513.26_533.3 513.26 533.3 10.07 67.2 25.1 SNP25_HUMAN NLTDLGK NLTDLGK_380.71_533.3 380.71 533.3 10.83 50.1 19.3 OLFL3_HUMAN TEADTISGR TEADTISGR_475.23_533.3 475.23 533.3 7.26 62.3 32.4

IC1_HUMAN TLYSSSPR TLYSSSPR_455.74_533.3 455.74 533.3 8.24 59.8 28.6 TNR19_HUMAN VLLEQEK VLLEQEK_429.75_533.3 429.75 533.3 9.97 56.4 24.4 ACHA5_HUMAN EETESGSGP EETESGSGPK_510.73_532.3 510.73 532.3 5.83 66.9 25 K MMP20_HUMAN EDVVSVVK EDVVSVVK_437.75_531.4 437.75 531.4 11.29 57.5 21.8 KCNQ1_HUMAN DNGVTPGEK DNGVTPGEK_458.72_531.3 458.72 531.3 6.25 60.2 22.7 P2RX1_HUMAN FDILVDGK FDILVDGK_453.75_531.3 453.75 531.3 16.55 59.5 22.5 HRG_HUMAN HSHESQDLR HSHESQDLR_370.18_531.3 370.18 531.3 5.96 48.8 19.4 FIBB_HUMAN SILENLR SILENLR_422.75_531.3 422.75 531.3 15.36 55.5 21.1 INADL_HUMAN SLVADGVAE SLVADGVAER_508.77_531.3 508.77 531.3 10.85 66.6 33.9 R CK041_HUMAN GTSSSPLAV GTSSSPLAVASGPAK_665.35 665.35 530.3 11.51 86.8 31.8 ASGPAK _530.3 ERLN1_HUMAN LLIAAQK LLIAAQK_378.75_530.3 378.75 530.3 11.11 49.9 19.2 GELS_HUMAN TGAQELLR TGAQELLR_444.2_530.3 444.2 530.3 11.1 58.3 25 BAX_HUMAN VPELIR VPELIR_363.73_530.3 363.73 530.3 13.05 47.9 24.5 FRS2_HUMAN DPQILLEPE DPQILLEPEGVK_669.37_ 669.37 529.3 16.71 87.3 41 GVK 529.3 ADA12_HUMAN FGFGGSTDS FGFGGSTDSGPIR_649.31_ 649.31 529.3 15.66 84.8 40.1 GPIR 529.3 PIN1_HUMAN NGQGEPAR NGQGEPAR_414.7_529.3 414.7 529.3 5.8 54.5 23.7 MAP4_HUMAN VALSSETEV VALSSETEVALAR_673.37_ 673.37 529.3 14.63 87.9 38.1 ALAR 529.3 DCBD1_HUMAN ASSSWQSVN ASSSWQSVNESGDQVHWS 834.05 528.3 14.41 108.6 49.1 ESGDQVHWS PGQAR_834.05_528.3 PGQAR SIX3_HUMAN DLYHILENH DLYHILENHK_427.89_527.3 427.89 527.3 16.71 56.2 19.4 K APOB_HUMAN IEGNLIFDP IEGNLIFDPNNYLPK_873.96 873.96 527.3 22.93 113.7 41 NNYLPK _527.3 NCAN_HUMAN LSSAIIAAP LSSAIIAAPR_499.8_527.3 499.8 527.3 13 65.5 24.5 R KCNA3_HUMAN SPPPPSAR SPPPPSAR_404.72_527.3 404.72 527.3 6.02 53.2 29.3 SO1A2_HUMAN YIYLGLPAA YIYLGLPAALR_625.37_ 625.37 527.3 22.53 81.7 30 LR 527.3 SEM4A_HUMAN EAILALDIQ EAILALDIQDPGVPR_803.94 803.94 525.3 20.26 104.7 46.9 DPGVPR _525.3 PZP_HUMAN AVGYLITGY AVGYLITGYQR_620.84_ 620.84 523.3 15.85 81.1 29.8 QR 523.3 NOE3_HUMAN SIADFVSGA SIADFVSGAESR_619.8_ 619.8 519.3 16.43 81 26.8 ESR 519.3 IL1R2_HUMAN TISASLGSR TISASLGSR_446.25_519.3 446.25 519.3 10.01 58.6 25.1 JPH3_HUMAN AEAALTAAQ AEAALTAAQK_487.27_ 487.27 518.3 7.79 63.9 23.9 K 518.3 LYG2_HUMAN EHLSQATGI EHLSQATGILTER_485.59_ 485.59 518.3 13.74 63.6 22.3 LTER 518.3 OTOAN_HUMAN GSSGSFLQP GSSGSFLQPDITER_747.37_ 747.37 518.3 15.66 97.4 44.4 DITER 518.3 PERL_HUMAN IHGFDLAAI IHGFDLAAINTQR_485.93_ 485.93 518.3 17.09 63.7 22.3 NTQR 518.3 OPCM_HUMAN NTGVSVGQK NTGVSVGQK_445.24_518.3 445.24 518.3 6.78 58.4 25.1 ITB5_HUMAN DDLDNIR DDLDNIR_430.71_517.3 430.71 517.3 10.48 56.6 21.5 GPC5_HUMAN LLEQVNR LLEQVNR_436.25_516.3 436.25 516.3 9.5 57.3 21.7 ARTN_HUMAN SDELVR SDELVR_359.69_516.3 359.69 516.3 8.28 47.4 21.3 GNAQ_HUMAN AHAQLVR AHAQLVR_397.74_515.3 397.74 515.3 6.97 52.3 26 ERO1B_HUMAN SPAAQYVDL SPAAQYVDLLLNPER_ 562.63 515.3 22.72 73.6 26.2 LLNPER 562.63_515.3 ACHA4_HUMAN SPSDQLPPQ SPSDQLPPQQPLEAEK_ 882.44 515.2 11.76 114.8 47.3 QPLEAEK 882.44_515.2 LAMB2_HUMAN AMDYDLLLR AMDYDLLLR_555.3_514.4 555.3 514.4 22 72.6 29.9 A1AG1_HUMAN YVGGQEHFA YVGGQEHFAHLLILR_ 584.99 514.4 19.31 76.5 33.4 HLLILR 584.99_514.4 ACHB_HUMAN AVALLPELR AVALLPELR_491.31_514.3 491.31 514.3 19.41 64.4 24.1 HCN2_HUMAN QFGALLQPG QFGALLQPGVNK_636.36_ 636.36 514.3 16.27 83.1 39.5 VNK 514.3 ACHB2_HUMAN IVPPTSLDV IVPPTSLDVPLVGK_717.93_ 717.93 513.3 19.09 93.6 43.1 PLVGK 513.3 HPT_HUMAN FTDHLK FTDHLK_380.7_512.3 380.7 512.3 8.41 50.1 19.3 CO6_HUMAN ENPAVIDFE ENPAVIDFELAPIVDLVR_ 1005.55 511.3 25.18 130.7 43.7 LAPIVDLVR 1005.55_511.3 LTBP1_HUMAN EEPVEALTF EEPVEALTFSR_639.32_510.3 639.32 510.3 16.79 83.5 27.6 SR SLIT1_HUMAN NLYINNELQ NLYINNELQDFTK_806.4_ 806.4 510.3 19.78 105 38 DFTK 510.3 BDNF_HUMAN THGTLESVN THGTLESVNGPK_413.88_ 413.88 510.3 8.23 54.4 18.7 GPK 510.3 GELS_HUMAN TASDFITK TASDFITK_441.73_508.3 441.73 508.3 10.75 58 27.9 NETO1_HUMAN YNFTPDPDF YNFTPDPDFK_622.29_506.3 622.29 506.3 16.1 81.3 35.9 K GBRB2_HUMAN GDDNAVTGV GDDNAVTGVTK_538.76_ 538.76 505.3 7.83 70.5 26.2 TK 505.3 PCSK1_HUMAN ALAHLLEAE ALAHLLEAER_374.88_504.2 374.88 504.2 14.09 49.4 19.7 R ACHA2_HUMAN LSPSYHWLE LSPSYHWLESNVDAEER_ 677.99 504.2 17.4 88.5 35.1 SNVDAEER 677.99_504.2 HAS1_HUMAN VGAVGGDVR VGAVGGDVR_415.23_503.3 415.23 503.3 7.58 54.6 20.8 CNTN1_HUMAN DGEYVVEVR DGEYVVEVR_533.26_502.3 533.26 502.3 12.46 69.8 26 UGGG2_HUMAN FLGPLDEDF FLGPLDEDFYAEDFYLLEK_ 1162.55 502.3 25.15 151 56.7 YAEDFYLLE 1162.55_502.3 K GP113_HUMAN WNLYEVVR WNLYEVVR_539.79_502.3 539.79 502.3 20.42 70.6 29.3 GPR88_HUMAN YLLITR YLLITR_389.74_502.3 389.74 502.3 14.87 51.3 19.6 ELAV3_HUMAN DANLYVSGL DANLYVSGLPK_588.82_ 588.82 501.3 15.26 77 28.4 PK 501.3 PAQR6_HUMAN FLELESPGL FLELESPGLSK_610.33_501.3 610.33 501.3 17.4 79.7 38.4 SK CRLF1_HUMAN LAGLKPGTV LAGLKPGTVYFVQVR_ 549.99 501.3 18.54 71.9 25.6 YFVQVR 549.99_501.3 T151A_HUMAN LVLGPEAAL LVLGPEAALAR_555.34_ 555.34 501.3 16.86 72.6 35.9 AR 501.3 ITA3_HUMAN TVEDVGSPL TVEDVGSPLK_522.78_501.3 522.78 501.3 11.35 68.4 25.5 K NCKX2_HUMAN VTAPEAQAK VTAPEAQAKPSAAR_466.26 466.26 501.3 6.95 61.1 30.3 PSAAR _501.3 IGF1R_HUMAN AENGPGPGV AENGPGPGVLVLR_639.86_ 639.86 500.4 16.44 83.5 39.7 LVLR 500.4 NFL_HUMAN VLEAELLVL VLEAELLVLR_577.86 500.4 577.86 500.4 21.86 75.5 27.9 R PCD18_HUMAN GDITLVPTI GDITLVPTINGTLPIR_840.49 840.49 500.3 21.43 109.4 36.5 NGTLPIR _500.3 PARD3_HUMAN TPIIWFHVV TPIIWFHVVPAANK_531.64_ 531.64 500.3 21.26 69.6 27.7 PAANK 500.3 PTPRO_HUMAN LIGLDIPHF LIGLDIPHFAADLPLNR_ 625.69 499.3 24.19 81.7 38.5 AADLPLNR 625.69_499.3 BTNL8_HUMAN LNGEHLYFT LNGEHLYFTLNPR_525.28_ 525.28 499.3 18.04 68.8 24.3 LNPR 499.3 PAQR6_HUMAN LPQLLQVHQ LPQLLQVHQVPR_476.62_ 476.62 499.3 16.86 62.5 30.9 VPR 499.3 KCNV2_HUMAN AQAQVEEAE AQAQVEEAEELFR_760.37_ 760.37 498.3 18.64 99.1 36 ELFR 498.3 PAR6B_HUMAN AVSTANPLL AVSTANPLLR_521.31_498.3 521.31 498.3 13.55 68.2 31.4 R ANGP1_HUMAN LEIQLLENS LEIQLLENSLSTYK_825.95_ 825.95 498.3 22.62 107.5 38.8 LSTYK 498.3 ADA23_HUMAN IGQLQGEII IGQLQGEIIPTSFYHQGR_ 682.03 497.3 18.63 89 38.3 PTSFYHQGR 682.03_497.3 GT253_HUMAN LDLTGSSGH LDLTGSSGHSLQPQPR_ 564.96 497.3 12.28 73.9 35.4 SLQPQPR 564.96_497.3 SIA8C_HUMAN YASPGAPR YASPGAPR_409.71_497.3 409.71 497.3 7.03 53.9 20.5 ERMIN_HUMAN LFIVHK LFIVHK_378.74_496.3 378.74 496.3 11.75 49.9 19.2 CB085_HUMAN LNPGIYPQQ LNPGIYPQQV_564.81_495.3 564.81 495.3 15.23 73.9 24.4 V AMPB_HUMAN NDHQEDFWK NDHQEDFWK_609.76_495.2 609.76 495.2 13.22 79.7 29.3 SCN2A_HUMAN FDPDATQFI FDPDATQFIEFAK_764.87_ 764.87 494.3 20.36 99.7 45.2 EFAK 494.3 SIX3_HUMAN AVVAFHTGN AVVAFHTGNFR_406.88_ 406.88 493.3 13.85 53.5 24.3 FR 493.3 MYP2_HUMAN FLGTWK FLGTWK_376.21_491.3 376.21 491.3 15.38 49.5 19.1 PACN1_HUMAN AGDELTK AGDELTK_367.19_490.3 367.19 490.3 6.7 48.4 21.7 SYUA_HUMAN EGVVAAAEK EGVVAAAEK_437.24_489.3 437.24 489.3 7.7 57.4 21.7 KCNA2_HUMAN FETQLK FETQLK_383.21_489.3 383.21 489.3 9.93 50.4 19.4 STH_HUMAN NGAEQGR NGAEQGR_366.18_489.2 366.18 489.2 5.65 48.2 21.6 APOL4_HUMAN LTATSTDQL LTATSTDQLEALR_709.88_ 709.88 488.3 14.6 92.6 42.7 EALR 488.3 FGFR3_HUMAN DGTGLVPSE DGTGLVPSER_515.76_488.2 515.76 488.2 10.4 67.5 25.2 R NEUM_HUMAN IEQDGIKPE IEQDGIKPEDK_636.33_488.2 636.33 488.2 7.45 83.1 36.5 DK RELN_HUMAN IISVELPGD IISVELPGDAK_571.33_487.3 571.33 487.3 16.13 74.7 36.6 AK

TNF6B_HUMAN LLQALR LLQALR_357.24_487.3 357.24 487.3 12.48 47.1 18.2 SO1B3_HUMAN LSLVGIAK LSLVGIAK_400.77_487.3 400.77 487.3 15.8 52.7 20.1 FETUA_HUMAN AHYDLR AHYDLR_258.8_487.2 258.8 487.2 8.05 34.4 13.7 PCDGF_HUMAN DLGFGVGDL DLGFGVGDLPTR_623.83_ 623.83 486.3 18.4 81.5 38.9 PTR 486.3 PCDBF_HUMAN GFPALSSEA GFPALSSEALVR_623.84_ 623.84 486.3 18.44 81.5 38.9 LVR 486.3 SIG12_HUMAN EGADIPWDI EGADIPWDIPVATNTPSGK_ 984.49 486.2 19.61 128 42.8 PVATNTPSG 984.49_486.2 K AP1B1_HUMAN LLSTDPVAA LLSTDPVAAK_507.8_485.3 507.8 485.3 11.18 66.5 30.8 K CLC4M_HUMAN LQEIYQELT LQEIYQELTIELK_753.91_ 753.91 484.3 20.81 98.3 32.7 ELK 484.3 NMDE3_HUMAN LLDVVTLEL LLDVVTLELGPGGPR_ 768.44 483.3 21.92 100.1 33.3 GPGGPR 768.44_483.3 ARTN_HUMAN ALGLGHR ALGLGHR_362.22_482.3 362.22 482.3 8.67 47.7 24.4 LRMP_HUMAN EYSSLPLPR EYSSLPLPR_531.29_482.3 531.29 482.3 14.85 69.5 25.9 PZP_HUMAN LEAGINQLS LEAGINQLSFPLSSEPIQGSY 1203.62 482.2 21.13 156.3 52.5 FPLSSEPIQ R_1203.62_482.2 GSYR NDF6_HUMAN QEETLDYGK QEETLDYGK_541.75_482.2 541.75 482.2 9.34 70.9 26.3 VTNC_HUMAN AVRPGYPK AVRPGYPK_296.51_481.3 296.51 481.3 7.09 39.2 15.7 COIA1_HUMAN AVGLAGTFR AVGLAGTFR_446.26_480.3 446.26 480.3 14.22 58.6 22.1 KCNA2_HUMAN EDEGYIK EDEGYIK_427.2_480.3 427.2 480.3 8.47 56.1 21.3 ACBD7_HUMAN ELYGLYK ELYGLYK_443.24_480.3 443.24 480.3 14.58 58.2 22 LAMB2_HUMAN IQGTLQPHA IQGTLQPHAR_374.21_480.3 374.21 480.3 7.74 49.3 22.6 R AMPB_HUMAN NDHQEDFWK NDHQEDFWK_609.76_480.3 609.76 480.3 13.22 79.7 32.3 CORT_HUMAN NFFWK NFFWK_371.19_480.3 371.19 480.3 18.71 48.9 18.8 EMIL3_HUMAN VHGLALGHE VHGLALGHEAHLQR_769.42 769.42 478.3 11.14 100.3 45.4 AHLQR _478.3 FIBG_HUMAN IHLISTQSA IHLISTQSAIPYALR_561.66_ 561.66 477.3 18.84 73.5 32.2 IPYALR 477.3 FEZ1_HUMAN GLSLQSSR GLSLQSSR_424.24_477.2 424.24 477.2 9.94 55.7 21.2 MMP1_HUMAN DIYSSFGFP DIYSSFGFPR_396.86_476.3 396.86 476.3 20.37 52.2 17.8 R PCDGE_HUMAN EYFTVNPES EYFTVNPESGDLLVSDR_ 970.97 476.2 19.4 126.3 45.2 GDLLVSDR 970.97_476.2 SAA4_HUMAN GPGGVWAAK GPGGVWAAK_421.73_475.3 421.73 475.3 11.21 55.4 21.1 APOD_HUMAN IPTTFENGR IPTTFENGR_517.77_475.2 517.77 475.2 11.08 67.8 34.3 LYG2_HUMAN AIKPYQTLI AIKPYQTLIK_392.25_474.3 392.25 474.3 13.84 51.6 17.6 K PCDB7_HUMAN DLGLGVGEL DLGLGVGELR_514.79_474.3 514.79 474.3 17.2 67.4 25.2 R NFL_HUMAN YEEEVLSR YEEEVLSR_512.75_474.3 512.75 474.3 10.61 67.1 34.1 WNK4_HUMAN AAEDSARPE AAEDSARPELPDSAVGPGS 661.32 473.2 11.97 86.3 31.3 LPDSRAVGP R_661.32_473.2 GS VGFR3_HUMAN HATLSLSIP HATLSLSIPR_365.55_472.3 365.55 472.3 15.55 48.2 16.2 R CBPN_HUMAN HLYVLEFSD HLYVLEFSDHPGIHEPLEPE 862.44 472.3 19.33 112.3 47.5 HPGIVKHEPL VK_862.44_472.3 EPE OLIG1_HUMAN IATLLLAR IATLLLAR_435.79_472.3 435.79 472.3 17.19 57.2 21.7 ITIH2_HUMAN IQPSGGTNIN IQPSGGTNINEALLR_791.93 791.93 472.3 15.42 103.2 46.3 EALLR _472.3 IL1R2_HUMAN QEYSENNENY QEYSENNENYIEVPLIFDPV 890.43 472.3 24.62 115.9 52 IEVPLIFDPV TR_890.43_472.3 TR IL1AP_HUMAN QIQVFEDEPA QIQVFEDEPAR_666.33_ 666.33 472.3 14.23 87 40.8 R 472.3 FRS2_HUMAN FVLGPTPVQK FVLGPTPVQK_543.32_471.3 543.32 471.3 14.71 71.1 35.4 CB085_HUMAN LNPGIYPQQV LNPGIYPQQV_564.81_471.3 564.81 471.3 15.23 73.9 27.4 FINC_HUMAN SSPVVIDAST S SPVVIDASTAIDAPSNLR_ 957 470.3 17.46 124.5 47.6 AIDAPSNLR 957_470.3 F123A_HUMAN DPAGEPAVGE DPAGEPAVGEEVPAPADR_ 888.92 470.2 11.94 115.7 41.6 EVPAPADR 888.92_470.2 CO2 HUMAN HAFILQDTK HAFILQDTK_536.79_469.3 536.79 469.3 12.44 70.2 32.1 JPH3_HUMAN WDLTFSPPQK WDLTFSPPQK_609.81_469.3 609.81 469.3 19.12 79.7 38.3 SYTL4_HUMAN ISAESPTGLP ISAESPTGLPSHK_441.9_ 441.9 468.3 9.66 58 23.1 SHK 468.3 ARHG7_HUMAN FNFQQTNEDE FNFQQTNEDELSFSK_917.42 917.42 468.2 17.52 119.3 51.9 LSFSK _468.2 LCN8_HUMAN FAFPGHR FAFPGHR_416.22_466.3 416.22 466.3 12.58 54.7 23.8 PRIO_HUMAN ESQAYYQR ESQAYYQR_522.74_466.2 522.74 466.2 7.39 68.4 22.5 MAMC2_HUMAN FTSQPGYIGR FTSQPGYIGR_563.29_464.2 563.29 464.2 11.01 73.7 24.3 HCN1_HUMAN DDGNSVFPAK DDGNSVFPAK_525.25_462.3 525.25 462.3 10.92 68.8 25.6 KIF5A_HUMAN TGAEGAVLDE TGAEGAVLDEAK_580.79_ 580.79 462.2 10.99 75.9 37.1 AK 462.2 PICAL_HUMAN VAEQVGIDR VAEQVGIDR_493.77_460.3 493.77 460.3 9.18 64.7 24.2 EAA2_HUMAN VTLAANGK VTLAANGK_387.23_460.3 387.23 460.3 7.55 51 19.5 AMNLS_HUMAN SGDEAPGLFF SGDEAPGLFFVDAER_ 805.38 460.2 20.88 104.9 34.9 VDAER 805.38_460.2 MPDZ_HUMAN HVLEDSPAGK HVLEDSPAGK_526.77_459.3 526.77 459.3 7.15 69 31.7 LGMN_HUMAN DYTGEDVTPQ DYTGEDVTPQNFLAVLR_ 646.66 458.3 24.14 84.4 30.5 NFLAVLR 646.66_458.3 PCDBC_HUMAN VGATDHGSPA VGATDHGSPALSSEALVR_ 589.64 458.3 13.35 77.1 27.6 LSSEALVR 589.64_458.3 PGCB_HUMAN ALHPEEDPEG ALHPEEDPEGR_625.29_ 625.29 458.2 7.71 81.7 39 R 458.2 SO1B3_HUMAN GIGETPIVPL GIGETPIVPLGISYIDDFAK_ 1053.06 458.2 25.06 136.8 45.8 GISYIDDFAK 1053.06_458.2 RELN_HUMAN FVYLELPAAA FVYLELPAAAK_611.35_ 611.35 457.3 19.69 79.9 38.4 K 457.3 HS3S5_HUMAN LITEPLPELQ LITEPLPELQLVEK_811.47_ 811.47 457.3 21.7 105.7 35.2 LVEK 457.3 E41L3_HUMAN TETISFGSVS TETISFGSVSPGGVK_733.38 733.38 457.3 16.02 95.6 43.8 PGGVK _457.3 S12A6_HUMAN IDDIPGLSDT IDDIPGLSDTSPDLSSR_ 894.44 457.2 16.94 116.4 38.9 SPDLSSR 894.44_457.2 COIA1_HUMAN ADDILASPPR ADDILASPPR_527.78_456.3 527.78 456.3 12.5 69.1 25.7 GOLM1_HUMAN DQLVIPDGQE DQLVIPDGQEEEQEAAGEG 1085.5 456.2 14.4 141 50.3 EREQEAAGEG R_1085.5_456.2 NECP2_HUMAN VRPASTGGLS VRPASTGGLSLLPPPPGGK_ 601.02 455.3 16.62 78.5 37.2 LLPPPPGGK 601.02_455.3 ASGR1_HUMAN WVDGTDYETG WVDGTDYETGFK_709.32_ 709.32 452.3 15.35 92.5 42.7 FK 452.3 LCK_HUMAN ERPEDRPTFD ERPEDRPTFDYLR_565.28_ 565.28 451.3 14.43 73.9 35.4 YLR 451.3 CATD_HUMAN YSQAVPAVTE YSQAVPAVTEGPIPEVLK_ 949.52 450.2 19.28 123.5 47.3 IPGPEVLK 949.52_450.2 ANGP1_HUMAN DAPHVEPDFS DAPHVEPDFSSQK_486.23_ 486.23 449.2 11 63.7 22.3 SQK 449.2 NMDE3_HUMAN SVEDALTSLK SVEDALTSLK_531.79_448.3 531.79 448.3 16.02 69.6 34.9 PCD18_HUMAN LLGEGFSDLF LLGEGFSDLFLTDGR_820.42 820.42 448.2 24.6 106.8 38.6 LTDGR _448.2 LRMP_HUMAN DDSEPSGEET DDSEPSGEETVER_725.3_ 725.3 447.1 7.41 94.6 31.4 VER 447.1 SPB5_HUMAN DVEDESTGLE DVEDESTGLEK_611.28_ 611.28 446.3 9.19 79.9 38.4 K 446.3 GOPC_HUMAN EAQLEAEVK EAQLEAEVK_508.77_446.3 508.77 446.3 10.15 66.6 24.9 PVRL1_HUMAN VEFLRPSFTD VEFLRPSFTDGTIR_546.63_ 546.63 446.3 18.3 71.5 34.4 GTIR 446.3 LIPE_HUMAN VVGHSIAR VVGHSIAR_419.75_446.3 419.75 446.3 6.61 55.1 30 GELS_HUMAN AQPVQVAEGS AQPVQVAEGSEPDGFWEAL 758.03 445.3 21.24 98.8 39.2 EPDGFWEALG GGK_758.03_445.3 GK MMP16_HUMAN LPNDDLQGIQ LPNDDLQGIQK_620.83_ 620.83 445.3 12.2 81.1 38.8 K 445.3 EAA2_HUMAN DLEVVAR DLEVVAR_401.23_444.3 401.23 444.3 10.94 52.8 20.2 P4K2A_HUMAN AYPFYWAWLP AYPFYWAWLPQAK_820.92 820.92 443.3 25.1 106.9 47.6 QAK _443.3 ERLN1_HUMAN EALEPSGENV EALEPSGENVIQNK_764.39_ 764.39 443.2 11.75 99.6 33.1 IQNK 443.2 KNG1_HUMAN DFVQPPTK DFVQPPTK_466.25_442.3 466.25 442.3 10.45 61.1 29 AT12A_HUMAN DGPNSLTPPK DGPNSLTPPK_513.27_442.3 513.27 442.3 9.55 67.2 25.1 LRP8_HUMAN NPLSELPVVK NPLSELPVVK_548.32_442.3 548.32 442.3 17.49 71.7 35.6 AGAP2_HUMAN YEQLLFLAPL YEQLLFLAPLSTSEEPLGR_ 721.72 442.3 24.92 94.1 43.4 ESTSEPLGR 721.72_442.3 YQ051_HUMAN GGVDLPATPG GGVDLPATPGGPR_597.32_ 597.32 442.2 12.41 78.1 25.8 GPR 442.2 SNTB1_HUMAN GSPQAGVDLS GSPQAGVDLSFATR_703.36 703.36 441.2 15.9 91.7 30.4 FATR _441.2 PTPRB_HUMAN TVVLQTDPLP TVVLQTDPLPPAR_703.9_ 703.9 440.3 14.83 91.8 30.5 PAR 440.3

APOC2_HUMAN TAAQNLYEK TAAQNLYEK_519.27_439.2 519.27 439.2 8.89 68 34.3 TMPS2_HUMAN VISHPNYDSK VISHPNYDSK_580.29_437.3 580.29 437.3 7.45 75.9 31 CO4B_HUMAN YIYGKPVQGV YIYGKPVQGVAYVR_538.3_ 538.3 437.3 13.9 70.4 25 AYVR 437.3 CO6_HUMAN ALNHLPLEYN ALNHLPLEYNSALYSR_ 930.98 436.2 17.62 121.1 52.5 SALYSR 930.98_436.2 ANT3_HUMAN ANRPFLVFIR ANRPFLVFIR_411.58_435.3 411.58 435.3 20.79 54.1 21.5 IL12B_HUMAN YENYTSSFFI YENYTSSFFIR_476.23_435.3 476.23 435.3 19.63 62.4 21.8 R GAS6_HUMAN EVFENDPETD EVFENDPETDYFYPR_ 960.92 435.2 18.37 125 41.8 YFYPR 960.92_435.2 F13A_HUMAN GTYIPVPIVS GTYIPVPIVSELQSGK_ 844.47 435.2 21.87 109.9 36.7 ELQSGK 844.47_435.2 NPT4_HUMAN EYIISSLK EYIISSLK_476.77_434.3 476.77 434.3 15.53 62.5 20.5 A4_HUMAN WYFDVTEGK WYFDVTEGK_572.77_434.2 572.77 434.2 18.68 74.9 36.7 CPLX2_HUMAN QALGGATK QALGGATK_373.21_433.2 373.21 433.2 6.4 49.1 21.9 ARP21_HUMAN VAAYFGLDH VAAYFGLDHNVDQTGK_ 578.95 433.2 14.89 75.7 30.1 NVDQTGK 578.95_433.2 KLK3_HUMAN HSQPWQVLV HSQPWQVLVASR_469.92_ 469.92 432.3 17.51 61.6 21.5 ASR 432.3 KCNA1_HUMAN VVINISGLR VVINISGLR_485.81_432.3 485.81 432.3 17.1 63.7 23.9 SNP25_HUMAN NLTDLGK NLTDLGK_380.71_432.2 380.71 432.2 10.83 50.1 22.3 MICA_HUMAN AKPQGQWAE AKPQGQWAEDVLGNK_ 547.62 431.3 15.39 71.6 25.5 DVLGNK 547.62_431.3 KCNQ1_HUMAN DNGVTPGEK DNGVTPGEK_458.72_430.2 458.72 430.2 6.25 60.2 31.7 ERO1B_HUMAN SVYRPLNPL SVYRPLNPLAPSR_490.61_ 490.61 430.2 14.64 64.3 25.6 APSR 430.2 C09A2_HUMAN VGDPGVAGL VGDPGVAGLPGEK_598.32_ 598.32 430.2 12.25 78.2 37.8 PGEK 430.2 S15A2_HUMAN IEDIPANK IEDIPANK_450.25_429.2 450.25 429.2 8.5 59.1 31.3 AMFR2_HUMAN SVEITTDN SVEITTDNILEGR_723.88_ 723.88 429.2 17.05 94.4 31.4 ILEGR 429.2 RGS11_HUMAN ELLEDPVGR ELLEDPVGR_514.27_428.3 514.27 428.3 13.83 67.3 31.1 LRP8_HUMAN EPEDPAPAL EPEDPAPALK_533.77_428.3 533.77 428.3 10.24 69.9 35 K RES18_HUMAN LQLLFPSET LQLLFPSETHSPLAK_560.98 560.98 428.3 19.65 73.4 35.2 HSPLAK _428.3 TNF6B_HUMAN LLQALEAPE LLQALEAPEGWGPTPR_ 867.96 426.3 19.64 113 40.7 GWGPTPR 867.96_426.3 LSHR_HUMAN LQALPSYGL LQALPSYGLESIQR_787.93_ 787.93 426.3 17.89 102.6 34.2 ESIQR 426.3 GPC5D_HUMAN LSPQQDAGG LSPQQDAGGV_486.24_426.2 486.24 426.2 9.7 63.7 20.9 V CO4B_HUMAN LQETSNWLL LQETSNWLLSQQQADGSFQ 1033.18 425.3 21.19 134.3 53.2 SQQQADGSF DLSPVIHR_1033.18_425.3 QDLSPVIHR ZIC1_HUMAN LVNHIR LVNHIR_251.16_425.3 251.16 425.3 7.04 33.4 16.4 APOB_HUMAN SPAFTDLHL SPAFTDLHLR_386.21_425.3 386.21 425.3 15.75 50.8 23.2 R CO4A4_HUMAN GDPGPPGAE GDPGPPGAEGPPGLPGK_ 750.38 424.2 11.98 97.8 38.5 GPPGLPGK 750.38_424.2 CAR14_HUMAN EQTLLQFQK EQTLLQFQK_567.81_422.2 567.81 422.2 15.65 74.2 27.5 HCN1_HUMAN GVPPAPPPP GVPPAPPPPAAALPR_704.41 704.41 422.2 13.39 91.9 33.5 AAALPR _422.2 HPCA_HUMAN FAEHVFR FAEHVFR_453.24_421.3 453.24 421.3 11.78 59.5 31.4 TM9S1_HUMAN IIHTDVFR IIHTDVFR_334.19_421.3 334.19 421.3 12.94 44.1 20.6 ALS_HUMAN SFEGLGQLE SFEGLGQLEVLTLDHNQLQ 833.1 421.2 23.16 108.5 40 VLTLDHNQL EVK_833.1_421.2 QEVK AGAL_HUMAN NFADIDDSW NFADIDDSWK_605.77_420.2 605.77 420.2 17.18 79.1 38.2 K TBB2B_HUMAN INVYYNEAT INVYYNEATGNK_693.34_ 693.34 419.2 11.92 90.4 42 GNK 419.2 KAIN_HUMAN WADLSGITK WADLSGITK_495.77_418.3 495.77 418.3 15.63 65 33.3 SAA4_HUMAN AEEWGR AEEWGR_374.17_418.2 374.17 418.2 8.8 49.3 22 P2RX1_HUMAN FDILVDGK FDILVDGK_453.75_418.2 453.75 418.2 16.55 59.5 22.5 GPC5D_HUMAN LSPQQDAGG LSPQQDAGGV_486.24_418.2 486.24 418.2 9.7 63.7 20.9 V CLUS_HUMAN EIQNAVNGV EIQNAVNGVK_536.3_417.2 536.3 417.2 7.9 70.2 32.1 K ERLN1_HUMAN LLIAAQK LLIAAQK_378.75_417.2 378.75 417.2 11.11 49.9 22.2 C1QL2_HUMAN IAFYVGLK IAFYVGLK_455.77_416.3 455.77 416.3 18.6 59.8 31.6 NKX62_HUMAN LNGLASSAG LNGLASSAGVYFGPAAAVA 946.51 416.3 19.6 123.1 44.1 VYFGPAAAV R_946.51_416.3 AR LGMN_HUMAN QLIEEIQR QLTEEIQR_508.77_416.3 508.77 416.3 9.87 66.6 24.9 LGI1_HUMAN WGGSSFQDI WGGSSFQDIQR_640.8_416.3 640.8 416.3 14.47 83.7 39.7 QR HEMO_HUMAN SGAQATWIE SGAQATWTELPWPHEK_ 613.3 415.2 19.54 80.1 25.8 LPWPHEK 613.3_415.2 NCAN_HUMAN LSSAIIAAP LSSAIIAAPR_499.8_414.2 499.8 414.2 13 65.5 24.5 R PTN5_HUMAN VLQAEELHE VLQAEELHEK_598.32_413.2 598.32 413.2 9.59 78.2 37.8 K KCNA1_HUMAN DFTGTVHR DFTGTVHR_466.73_411.2 466.73 411.2 8.37 61.2 29 CALL3_HUMAN EAFSLFDK EAFSLFDK_478.74_409.2 478.74 409.2 18.7 62.8 23.6 NEUG_HUMAN IQASFR IQASFR_361.2_409.2 361.2 409.2 9.77 47.6 21.4 SYTL4_HUMAN YEIPESLLA YEIPESLLAQR_659.85_406.2 659.85 406.2 17.79 86.1 28.5 QR LYG2_HUMAN EHLSQATGI EHLSQATGILTER_485.59_ 485.59 405.2 13.74 63.6 22.3 LTER 405.2 P4K2A_HUMAN IYQGSSGSY IYQGSSGSYFVK_668.33_ 668.33 405.2 13.39 87.2 28.9 FVK 405.2 A4_HUMAN LVFFAEDVG LVFFAEDVGSNK_663.34_ 663.34 405.2 19.15 86.6 40.7 SNK 405.2 LTBP1_HUMAN TSTDLDVDV TSTDLDVDVDQPK_716.84_ 716.84 405.2 12.25 93.5 37 DQPK 405.2 NTRK2_HUMAN NLTIVDSGL NLTIVDSGLK_530.31_404.3 530.31 404.3 15.17 69.4 34.8 K FA20A_HUMAN HNAEIAAFH HNAEIAAFHLDR_465.24_ 465.24 403.2 14.27 61 27.3 LDR 403.2 GNAQ_HUMAN YYLNDLDR YYLNDLDR_536.26_403.2 536.26 403.2 14.39 70.2 35.1 NMDZ1_HUMAN VLQFDPGTK VLQFDPGTK_502.77_402.2 502.77 402.2 13.24 65.9 30.6 BAX_HUMAN VPELIR VPELIR_363.73_401.3 363.73 401.3 13.05 47.9 27.5 GAS6_HUMAN IAVAGDLFQ IAVAGDLFQPER_658.35_ 658.35 401.2 17.91 85.9 31.5 PER 401.2 TLR7_HUMAN NLETLDLSH NLETLDLSHNQLTTVPER_ 694.03 401.2 16.88 90.5 38.9 NQLTTVPER 694.03_401.2 ERO1B_HUMAN SPAAQYVDL SPAAQYVDLLLNPER_ 562.63 401.2 22.72 73.6 26.2 LLNPER 562.63_401.2 C09A2_HUMAN GEQGPPGIP GEQGPPGIPGPQGLPGVK_ 842.95 400.3 16.02 109.7 42.6 GPQGLPGVK 842.95_400.3 ITAM_HUMAN VQSLVLGAP VQSLVLGAPR_520.32_400.2 520.32 400.2 13.93 68.1 25.4 R ITA3_HUMAN YLLLAGAPR YLLLAGAPR_487.3_400.2 487.3 400.2 17.15 63.9 20.9 LAT3_HUMAN LSGLALDHK LSGLALDHK_318.52_399.2 318.52 399.2 11.36 42.1 19.8 TAU_HUMAN TPSLPTPPT TPSLPTPPTR_533.8_399.2 533.8 399.2 12.13 69.9 26 R SGCZ_HUMAN LGNLPTGSF LGNLPTGSFSSSSPSSSSSR_ 971.46 398.2 13.75 126.3 45.2 SSSSPSSSS 971.46_398.2 SR RUN3A_HUMAN TPVVIDYTP TPVVIDYTPYLK_704.89_ 704.89 397.2 18.83 91.9 33.5 YLK 397.2 SLIT1_HUMAN NLYINNELQ NLYINNELQDFTK_806.4_ 806.4 391.2 19.78 105 38 DFTK 391.2 DDR2_HUMAN NLYSGDYYR NLYSGDYYR_575.76_391.2 575.76 391.2 12.58 75.3 27.8 NDF6_HUMAN NYIWALSEI NYIWALSEILR_689.38_391.2 689.38 391.2 25.16 89.9 29.8 LR SCG1_HUMAN NYLNYGEEG NYLNYGEEGAPGK_706.33_ 706.33 391.2 12.31 92.1 33.6 APGK 391.2 SYUA_HUMAN EGVLYVGSK EGVLYVGSK_476.26_390.2 476.26 390.2 12.17 62.4 23.5 UGGG2_HUMAN FLGPLDEDF FLGPLDEDFYAEDFYLLEK_ 1162.55 389.2 25.15 151 56.7 FYAEDYLLE 1162.55_389.2 K ASGR1_HUMAN SLESQLEK SLESQLEK_467.25_389.2 467.25 389.2 11.96 61.3 29.1 GP2_HUMAN FVGEGGVR FVGEGGVR_410.72_388.2 410.72 388.2 9.22 54 29.6 ACE2_HUMAN SGENPYASI SGENPYASIDISK_690.84_ 690.84 388.1 13.96 90.1 32.9 DISK 388.1 ARTN_HUMAN SDELVR SDELVR_359.69_387.3 359.69 387.3 8.28 47.4 21.3 TFR2_HUMAN YGDVVLR YGDVVLR_411.23_387.3 411.23 387.3 11.84 54 20.6 PCD18_HUMAN GDITLVPTI GDITLVPTINGTLPIR_840.49 840.49 387.2 21.43 109.4 48.5 NGTLPIR _387.2 YQ051_HUMAN SLADPPSSS SLADPPSSSQALHSPAR_ 574.29 387.2 10.78 75.1 23.8 QALHSPAR 574.29_387.2 NETR_HUMAN WAEVPPFLE WAEVPPFLER_622.33_387.2 622.33 387.2 20.93 81.3 29.9 R BEST1_HUMAN WWNQYENLP WWNQYENLPWPDR_902.42 902.42 387.2 21.96 117.4 39.2 WPDR _387.2 SCG1_HUMAN GEAGAPGEE GEAGAPGEEDIQGPTK_ 778.37 386.2 9.56 101.4 33.7 DIQGPTK 778.37_386.2 BTNL8_HUMAN LNGEHLYFT LNGEHLYFTLNPR_525.28_ 525.28 386.2 18.04 68.8 24.3 LNPR 386.2 EDNRB_HUMAN LTLYNQNDP LTLYNQNDPNR_674.34_ 674.34 386.2 11.47 88 41.2 NR 386.2

KAIN_HUMAN FSISGSYVL FSISGSYVLDQILPR_565.64_ 565.64 385.3 24.79 74 23.4 DQILPR 385.3 HCN2_HUMAN AEALPPEAA AEALPPEAADEGGPR_ 740.36 385.2 10.48 96.5 32.1 DEGGPR 740.36_385.2 MMP16_HUMAN YGYLPPTDP YGYLPPTDPR_589.8_384.2 589.8 384.2 12.72 77.1 28.5 R ATS1_HUMAN GPEVTSNAA GPEVTSNAALTLR_664.86_ 664.86 383.2 14.03 86.8 31.8 LTLR 383.2 ZIC2_HUMAN LGLPGEVFG LGLPGEVFGR_522.8_381.3 522.8 381.3 18.49 68.4 25.5 R APOE_HUMAN LGPLVEQGR LGPLVEQGR_484.78_381.3 484.78 381.3 11.76 63.5 23.8 MYP2_HUMAN NTEISFK NTEISFK_419.72_381.2 419.72 381.2 11.93 55.1 27 KNG1_HUMAN TVGSDTFYS TVGSDTFYSFK_626.3_381.2 626.3 381.2 16.46 81.8 39.1 FK DIRA2_HUMAN ESYIPTVED ESYIPTVEDTYR_736.85_ 736.85 380.1 15.41 96.1 34.9 TYR 380.1 MAP4_HUMAN TDYIPLLDV TDYIPLLDVDEK_710.86_ 710.86 380.1 20.87 92.7 33.8 DEK 380.1 PCDGE_HUMAN EYFTVNPES EYFTVNPESGDLLVSDR_ 970.97 377.2 19.4 126.3 42.2 GDLLVSDR 970.97_377.2 SIA8C_HUMAN FLDPSFVPI FLDPSFVPITNSLTQELQEKP 873.46 376.2 23.48 113.7 39.1 TNSLTQELQ SK_873.46_376.2 EKPSK DCBD2_HUMAN NNFLPPIIA NNFLPPIIAR_577.84_376.2 577.84 376.2 19.88 75.5 27.9 R CHAD_HUMAN NNFPVLAAN NNFPVLAANSFR_675.35_ 675.35 376.2 18.58 88.1 29.2 SFR 376.2 C1QT4_HUMAN VPGAYFFSF VPGAYFFSFTAGK_464.57_ 464.57 376.2 22.38 60.9 21.2 TAGK 376.2 PDIA2_HUMAN EDGILVLSR EDGILVLSR_501.29_375.2 501.29 375.2 16.44 65.7 24.6 AT2A2_HUMAN EFDELNPSA EFDELNPSAQR_653.31_ 653.31 374.2 12.74 85.3 37.2 QR 374.2 A2MG_HUMAN IAQWQSFQL IAQWQSFQLEGGLK_802.93 802.93 374.2 19.82 104.6 40.8 EGGLK _374.2 ACE2_HUMAN SEPWTLALE SEPWTLALENVVGAK_ 538.62 374.2 23.88 70.5 28 NVVGAK 538.62_374.2 SCNNA_HUMAN NGSDVPVEN NGSDVPVENLYPSK_759.88 759.88 374.1 14.27 99 38.9 LYPSK _374.1 PCDBF_HUMAN GFPALSSEA GFPALSSEALVR_623.84_ 623.84 373.2 18.44 81.5 29.9 LVR 373.2 APLP1_HUMAN HYQHVAAVD HYQHVAAVDPEK_465.23_ 465.23 373.2 7.77 61 27.3 PEK 373.2 SIG12_HUMAN EGADIPWDI EGADIPWDIPVATNTPSGK_ 984.49 373.1 19.61 128 48.8 PVATNTPSG 984.49_373.1 K FIBB_HUMAN IRPFFPQQ IRPFFPQQ_516.79_372.2 516.79 372.2 16.47 67.7 22.2 BCAS1_HUMAN VDEVPGLSG VDEVPGLSGQSDDVPAGK_ 85.43 372.2 13.15 115.2 47.5 QSDDVPAGK 885.43_372.2 CLC4M_HUMAN LQEIYQELT LQEIYQELTELK_753.91_ 753.91 371.2 20.81 98.3 38.7 ELK 371.2 TLR7_HUMAN LQELDLSQN LQELDLSQNFLAK_759.91_ 759.91 371.2 19.89 99 35.9 FLAK 371.2 PCDBC_HUMAN ALDYEERPE ALDYEERPELSFILTALDGG 882.79 369.2 25.09 114.9 39.6 LSFILTALD SPPR_882.79_369.2 GGSPPR BEST1_HUMAN FLGLQSHDH FLGLQSHDHHPPR_514.26_ 514.26 369.2 11.25 67.3 32.8 HPPR 369.2 ITIH4_HUMAN GPDVLTATV GPDVLTATVSGK_572.81_ 572.81 369.2 13.31 74.9 27.7 SGK 369.2 PCDBF_HUMAN LTLTAVDGG LTLTAVDGGSPPR_428.57_ 428.57 369.2 13.73 56.3 19.4 SPPR 369.2 PGCP_HUMAN AIINLAVYG AIINLAVYGK_531.32_367.2 531.32 367.2 17.88 69.5 34.9 K NDF6_HUMAN QEETLDYGK QEETLDYGK_541.75_367.2 541.75 367.2 9.34 70.9 35.3 MICA_HUMAN ASGFYPWNI SWRASGFYPWNITLSWR_849.43 849.43 363.2 25.08 110.6 39.9 TL _363.2 INHBA_HUMAN SELLLSEK SELLLSEK_459.76_363.2 459.76 363.2 13.55 60.3 31.7 CP46A_HUMAN ALQTVFGER ALQTVFGER_510.78_361.2 510.78 361.2 14.69 66.9 25 AFAM_HUMAN HELTDEELQ HELTDEELQSLFTNFANVV 817.07 361.2 25.08 106.4 36.2 SLFTNFANV DK_817.07_361.2 VDK TYRO_HUMAN LTGDENFTI LTGDENFTIPYWDWR_ 956.95 361.2 23.81 124.4 53.6 PYWDWR 956.95_361.2 CRLF1_HUMAN VGGLEDQLS VGGLEDQLSVR_586.82_ 586.82 361.2 14.23 76.7 28.3 VR 361.2 CRUM1_HUMAN GDVIYIGGL GDVIYIGGLPDK_623.84_ 623.84 359.2 17.2 81.5 38.9 PDK 359.2 SREC_HUMAN GTQGSELDP GTQGSELDPK_516.25_359.2 516.25 359.2 7.72 67.6 34.2 K APOL4_HUMAN LTATSTDQL LTATSTDQLEALR_709.88_ 709.88 359.2 14.6 92.6 36.7 EALR 359.2 CEL3B_HUMAN LYTNGPLPD LYTNGPLPDK_559.3_359.2 559.3 359.2 11.76 73.1 36.1 K ERO1B_HUMAN SVYRPLNPL SVYRPLNPLAPSR_490.61_ 490.61 359.2 14.64 64.3 22.6 APSR 359.2 DCBD2_HUMAN YSSSEVNHL YSSSEVNHLSPR_459.23_ 459.23 359.2 9.61 60.2 27 SPR 359.2 UNC5C_HUMAN NEDIIDPVE NEDIIDPVEDR_657.81_359.1 657.81 359.1 13.99 85.9 37.4 DR APLP1_HUMAN DELAPAGTG DELAPAGTGVSR_586.8_ 586.8 358.2 9.53 76.7 28.3 VSR 358.2 CO8A_HUMAN SLLQPNK SLLQPNK_400.24_358.2 400.24 358.2 10.77 52.6 26.1 CD3D_HUMAN NDQVYQPLR NDQVYQPLR_566.79_358.1 566.79 358.1 11.59 74.1 27.4 ITIH1_HUMAN EVAFDLEIP EVAFDLEIPK_580.81_357.3 580.81 357.3 19.77 75.9 25.1 K ERMIN_HUMAN ITEQPLK ITEQPLK_414.75_357.3 414.75 357.3 8.64 54.5 29.7 EPHA8_HUMAN DLGASTQES DLGASTQESQFLK_712.36_ 712.36 357.2 14.58 92.9 30.8 QFLK 357.2 GOLM1_HUMAN DQLVIPDGQ DQLVIPDGQEEEQEAAGEG 1085.5 357.2 14.4 141 56.3 EEEQEAAGE R_1085.5_357.2 GR PON1_HUMAN IQNILTEEP IQNILTEEPK_592.83_356.2 592.83 356.2 14.3 77.5 28.6 K CAD22_HUMAN LLEPESEFI LLEPESEFIIK_659.37_356.2 659.37 356.2 19.62 86.1 28.5 IK GBRA2_HUMAN LNQYDLLGQ LNQYDLLGQSIGK_724.89_ 724.89 356.2 17.87 94.5 34.4 SIGK 356.2 ITB2_HUMAN LGAILTPND LGAILTPNDGR_563.81_ 563.81 355.2 13.18 73.7 27.3 GR 355.2 RES18_HUMAN LQLLFPSET LQLLFPSETHSPLAK_560.98 560.98 355.2 19.65 73.4 26.2 HSPLAK _355.2 41_HUMAN APIAAPEPE APIAAPEPELK_568.32_353.2 568.32 353.2 12.69 74.3 30.5 LK ASGR1_HUMAN WVDGTDYET WVDGTDYETGFK_709.32_ 709.32 351.2 15.35 92.5 42.7 GFK 351.2 CADH7_HUMAN FLSLGPFSD FLSLGPFSDTTTVK_756.9_ 756.9 348.2 21.28 98.6 35.8 TTTVK 348.2 ANO3_HUMAN IAYLLTNLE IAYLLTNLEYPR_733.41_ 733.41 348.2 21.46 95.6 37.8 YPR 348.2 S12A5_HUMAN ETTAGHLAL ETTAGHLALLVTK_451.93_ 451.93 347.2 15.98 59.3 26.6 LVTK 347.2 ELAV3_HUMAN GLNGQKPLG GLNGQKPLGAAEPITVK_ 564.99 347.2 13.41 73.9 35.4 AAEPITVK 564.99_347.2 KCNA3_HUMAN SNSTLSK SNSTLSK_368.7_347.2 368.7 347.2 6 48.6 24.7 A1BG_HUMAN LETPDFQLF LETPDFQLFK_619.33_344.2 619.33 344.2 21.25 80.9 26.8 K ANT3_HUMAN DDLYVSDAF DDLYVSDAFHK_655.31_ 655.31 344.1 14.4 85.5 34.3 HK 344.1 SLIK1_HUMAN DIDPGAFQD DIDPGAFQDLNK_666.83_ 666.83 344.1 15.66 87 28.8 LNK 344.1 S12A6_HUMAN IDDIPGLSD IDDIPGLSDTSPDLSSR_ 894.44 344.1 16.94 116.4 44.9 TSPDLSSR 894.44_344.1 BCAS1_HUMAN VDEVPGLSG VDEVPGLSGQSDDVPAGK_ 885.43 344.1 13.15 115.2 50.5 QSDDVPAGK 885.43_344.1 INADL_HUMAN DVQPGSVAD DVQPGSVADR_522.26_343.2 522.26 343.2 7.7 68.4 22.5 R LGI1_HUMAN FQELNVQAP FQELNVQAPR_601.32_343.2 601.32 343.2 13.58 78.6 29 R LSHR_HUMAN LSLAYLPVK LSLAYLPVK_502.31_343.2 502.31 343.2 18.88 65.8 33.6 PIN1_HUMAN NGQGEPAR NGQGEPAR_414.7_343.2 414.7 343.2 5.8 54.5 20.7 CF072_HUMAN NVETTVDED NVETTVDEDVLPGK_758.38 758.38 343.2 14.91 98.8 35.9 VLPGK _343.2 NMS_HUMAN TQEATHPVK TQEATHPVK_505.77_343.2 505.77 343.2 5.87 66.2 30.8 AT2B3_HUMAN ILDLLVHAI ILDLLVHAISINSAYTTK_ 658.04 342.2 24.28 85.9 37.1 SINSAYTTK 658.04_342.2 AMNLS_HUMAN ILDTFLGLP ILDTFLGLPQYHGLQVAVS 733.74 342.2 22.88 95.7 32 QYHGLQVAV K_733.74_342.2 SK PSMG1_HUMAN LDLITVEAF LDLITVEAFKPILSTR_606.02 606.02 342.2 23.92 79.2 25.5 KPILSTR _342.2 NMDE3_HUMAN LLDVVTLEL LLDVVTLELGPGGPR_ 768.44 342.2 21.92 100.1 45.3 GPGGPR 768.44_342.2 PACN1_HUMAN NNLLNEDLE NNLLNEDLEK_601.31_342.2 601.31 342.2 14.37 78.6 26 K CD72_HUMAN VLEVTNSSL VLEVTNSSLR_559.31_342.2 559.31 342.2 12.4 73.2 27.1 R AT12A_HUMAN DGPNSLTPP DGPNSLTPPK_513.27_341.2 513.27 341.2 9.55 67.2 28.1 K IL12B_HUMAN DIIKPDPPK DIIKPDPPK_511.8_341.2 511.8 341.2 9.7 67 28 HAS1_HUMAN ILNPLDSWV ILNPLDSWVSFLSSLR_ 924.01 341.2 25.49 120.2 40.2

SFLSSLR 924.01_341.2 TRIM9_HUMAN NILVQTPES NILVQTPESESPQSHR_ 607.97 341.2 11.95 79.4 28.6 ESPQSHR 607.97_341.2 FRS2_HUMAN DPQILLEPE DPQILLEPEGVK_669.37_ 669.37 341.1 16.71 87.3 32 GVK 341.1 5HT2C_HUMAN ELNVNIYR ELNVNIYR_510.78_338.2 510.78 338.2 14.34 66.9 22 PCDGF_HUMAN YGPQFTLQH YGPQFTLQHVPDYR_574.29 574.29 338.2 16.79 75.1 35.8 VPDYR _338.2 SCG3_HUMAN LNVEDVDST LNVEDVDSTK_560.28_335.2 560.28 335.2 11 73.3 36.2 K ABCA1_HUMAN FVSPLSWDL FVSPLSWDLVGR_688.37_ 688.37 334.2 23.76 89.8 29.8 VGR 334.2 PERL_HUMAN FWWENPGVF FWWENPGVFTNEQK_ 891.42 334.2 22.67 116 41.7 TNEQK 891.42_334.2 CFAB_HUMAN YGLVTYATY YGLVTYATYPK_638.33 638.33 334.2 15.87 83.3 30.6 PK _334.2 CETP_HUMAN ASYPDITGE ASYPDITGEK_540.76_333.2 540.76 333.2 11.23 70.8 35.3 K SCNNA_HUMAN DNNPQVDWK DNNPQVDWK_558.26_333.2 558.26 333.2 11.27 73 36.1 MYP2_HUMAN FLGTWK FLGTWK_376.21_333.2 376.21 333.2 15.38 49.5 25.1 CORT_HUMAN NFFWK NFFWK_371.19_333.2 371.19 333.2 18.71 48.9 18.8 SEMG1_HUMAN LPSEFSQFP LPSEFSQFPHGQK_501.25_ 501.25 332.2 14.66 65.7 29.1 HGQK 332.2 FIBG_HUMAN LTIGEGQQH LTIGEGQQHHLGGAK_ 515.94 332.2 9.73 67.6 32.9 HLGGAK 515.94_332.2 HRG_HUMAN ADLFYDVEA ADLFYDVEALDLESPK_ 912.95 331.2 23.8 118.8 42.7 LDLESPK 912.95_331.2 S39A4_HUMAN AGLWASHAD AGLWASHADHLLALLESPK 677.03 331.2 24.55 88.3 41.1 HLLALLESP _677.03_331.2 K SIA8C_HUMAN FLDPSFVPI FLDPSFVPITNSLTQELQEKP 873.46 331.2 23.48 113.7 51.1 TNSLTQELQ SK_873.46_331.2 EKPSK RES18_HUMAN GFSGPLPTV GFSGPLPTVGR_544.3_331.2 544.3 331.2 15.06 71.2 26.4 GR RGS20_HUMAN IIYEDYISI IIYEDYISIL SPK_777.43_ 777.43 331.2 22.75 101.3 33.7 LSPK 331.2 GFRA2_HUMAN NAIQAFGNG NAIQAFGNGTDVNVSPK_ 866.44 331.2 14.98 112.8 37.6 TDVNVSPK 866.44_331.2 SCNNA_HUMAN NGSDVPVEN NGSDVPVENLYPSK_759.88 759.88 331.2 14.27 99 32.9 LYPSK _331.2 CLUS_HUMAN SGSGLVGR SGSGLVGR_366.7_331.2 366.7 331.2 7.52 48.3 24.6 NTRK2_HUMAN SNEIPSTDV SNEIPSTDVTDK_653.31_ 653.31 331.1 10.04 85.3 34.2 TDK 331.1 AT2A2_HUMAN WGSNELPAE WGSNELPAEEGK_658.81_ 658.81 331.1 12.28 86 34.5 EGK 331.1 INHBA_HUMAN SELLLSEK SELLLSEK_459.76_330.2 459.76 330.2 13.55 60.3 22.7 CB085_HUMAN GSLALPFPA GSLALPFPADVQGK_700.38 700.38 329.2 19.32 91.3 33.3 DVQGK _329.2 PGCP_HUMAN LALLVDTVG LALLVDTVGPR_577.35_ 577.35 329.2 18.84 75.5 24.9 PR 329.2 PEDF_HUMAN LQSLFDSPD LQSLFDSPDFSK_692.34_ 692.34 329.2 18.9 90.3 33 FSK 329.2 APOL4_HUMAN NLTPYVAIE NLTPYVAIEDK_631.84_ 631.84 329.2 15.55 82.5 30.3 DK 329.2 ARSA_HUMAN QSLFFYPSY QSLFFYPSYPDEVR_874.42_ 874.42 329.2 21.26 113.8 47 PDEVR 329.2 CP46A HUMAN AEQLVEILE AEQLVEILEAK_621.85_ 621.85 329.1 20.05 81.2 29.9 AK 329.1 MOT2_HUMAN LVDLTGEYK LVDLTGEYK_519.28_328.2 519.28 328.2 13.84 68 25.3 GPC5_HUMAN NAAAFQETL NAAAFQETLETLIK_774.92_ 774.92 328.2 24.18 101 33.6 ETLIK 328.2 AT12A_HUMAN TPEIYSVEL TPEIYSVELSGTK_712.37_ 712.37 328.2 15.53 92.9 36.8 SGTK 328.2 WNT8B_HUMAN VDLLQGAGN VDLLQGAGNSAAGR_ 664.85 328.2 13.4 86.8 31.8 SAAGR 664.85_328.2 CAD11_HUMAN VLDVNDNAP VLDVNDNAPK_542.79_ 542.79 328.2 9.67 71 26.4 K 328.2 VTNC_HUMAN AVRPGYPK AVRPGYPK_296.51_327.2 296.51 327.2 7.09 39.2 18.7 TBB2B_HUMAN INVYYNEAT INVYYNEATGNK_693.34_ 693.34 327.2 11.92 90.4 33 GNK 327.2 A1BG_HUMAN TPGAAANLE TPGAAANLELIFVGPQHAG 766.07 327.2 21.76 99.8 39.6 LIFVGPQHA NYR_766.07_327.2 GNYR PCDB7_HUMAN ILVLDVNDN ILVLDVNDNAPDFVR_ 850.45 326.2 20.44 110.7 39.9 APDFVR 850.45_326.2 EMIL2 HUMAN LIVPEPDVD LIVPEPDVDFDAK_729.38_ 729.38 326.2 18.52 95.1 31.6 FDAK 326.2 LRP8_HUMAN NPLSELPVV NPLSELPVVK_548.32_325.2 548.32 325.2 17.49 71.7 26.6 K APOOL_HUMAN HSVPLPTEL HSVPLPTELSSEAK_498.93_ 498.93 324.2 13.72 65.4 32 SSEAK 324.2 GOPC_HUMAN EHGVPILIS EHGVPILISEIHPGQPADR_ 689.03 324.1 17.44 89.9 41.7 EIHPGQPAD 689.03_324.1 R PGCB_HUMAN ALHPEEDPE ALHPEEDPEGR_625.29_ 625.29 322.2 7.71 81.7 36 GR 322.2 ACHB2_HUMAN EGAGALFFR EGAGALFFR_484.25_322.2 484.25 322.2 18.49 63.5 20.8 SLIT1_HUMAN QIPLQDVAF QIPLQDVAFPDFR_773.41_ 773.41 322.2 22.44 100.8 45.5 PDFR 322.2 C1QL2_HUMAN SPHEGYEVL SPHEGYEVLK_386.86_322.2 386.86 322.2 10.69 50.9 23.3 K F13A_HUMAN GTYIPVPIV GTYIPVPIVSELQSGK_ 844.47 322.1 21.87 109.9 36.7 SELQSGK 844.47_322.1 NECP2_HUMAN AAEWQLDQP AAEWQLDQPSWSGR_ 815.88 319.2 17.32 106.2 35.4 SWSGR 815.88_319.2 5HT2C_HUMAN VAATALSGR VAATALSGR_423.25_319.2 423.25 319.2 8.46 55.6 30.1 CFAH_HUMAN NGFYPATR NGFYPATR_463.23_319.1 463.23 319.1 11.09 60.8 22.9 FGL1_HUMAN HGEYWLGNK HGEYWLGNK_552.27_318.2 552.27 318.2 12.65 72.2 32.8 RGS20_HUMAN LFGLLSSPL LFGLLSSPLSSLAR_730.93_ 730.93 318.2 25.08 95.3 40.7 SSLAR 318.2 KCTD4_HUMAN YPDTFLEGI YPDTFLEGIVNGK_726.87_ 726.87 318.2 21.72 94.8 43.5 VNGK 318.2 LRMP_HUMAN DDSEPSGEE DDSEPSGEETVER_725.3_ 725.3 318.1 7.41 94.6 34.4 TVER 318.1 PLCB1_HUMAN NDSIPQEDF NDSIPQEDFTPEVYR_905.42 905.42 317.1 16.36 117.8 42.3 TPEVYR _317.1 CFAB_HUMAN DISEVVTPR DISEVVTPR_339.19_316.2 339.19 316.2 12.19 44.8 17.8 GRM5_HUMAN DSLISSEEE DSLISSEEEEGLVR_781.88_ 781.88 316.2 16 101.9 33.9 EGLVR 316.2 AGAP2_HUMAN SLDLDDWPR SLDLDDWPR_558.77_316.2 558.77 316.2 18.55 73.1 27.1 PCDB6_HUMAN SLDYEALQS SLDYEALQSFEFR_802.88_ 802.88 316.2 21.86 104.6 43.8 FEFR 316.2 AMFR2_HUMAN SVEITTDNI SVEITTDNILEGR_723.88_ 723.88 316.2 17.05 94.4 34.4 LEGR 316.2 MMP24_HUMAN ADEAEAPFA ADEAEAPFAGQNWLK_ 823.89 316.1 19.35 107.3 47.8 GQNWLK 823.89_316.1 NMDE1_HUMAN DEAVLILSE DEAVLILSEAR_608.33_ 608.33 316.1 17.94 79.5 35.3 AR 316.1 HCN1_HUMAN DDGNSVFPA DDGNSVFPAK_525.25_315.2 525.25 315.2 10.92 68.8 28.6 K AMPB_HUMAN ETFASTASQ ETFASTASQLHSNVVNYVQ 911.14 315.2 24.72 118.5 41 LHSNVVNYV QIVAPK_911.14_315.2 QQIVAPK APOOL_HUMAN LGSSSEIEV LGSSSEIEVPAK_608.82_ 608.82 315.2 11.43 79.5 35.3 PAK 315.2 P2RX1_HUMAN NLSPGFNFR NLSPGFNFR_526.27_315.2 526.27 315.2 16.65 68.9 22.7 FIBA_HUMAN NSLFEYQK NSLFEYQK_514.76_315.2 514.76 315.2 14.01 67.4 22.1 ADA23_HUMAN AWGAAAPSA AWGAAAPSAPHWNETAEK 631.97 315.1 14.4 82.5 29.8 PHWNETAEK _631.97_315.1 BCAS1_HUMAN DPTLLPPET DPTLLPPETGGAGGEAPSKP 673.68 314.1 12.9 87.9 40.9 GGAGGEAPS K_673.68_314.1 KPK A2MG_HUMAN IAQWQSFQL IAQWQSFQLEGGLK_802.93 802.93 313.2 19.82 104.6 37.8 EGGLK _313.2 PAK3_HUMAN LAKPLSSLT LAKPLSSLTPLIIAAK_818.52 818.52 313.2 21.28 106.6 41.5 PLIIAAK _313.2 LSHR_HUMAN LQALPSYGL LQALPSYGLESIQR_787.93_ 787.93 313.2 17.89 102.6 40.2 ESIQR 313.2 GBRA2_HUMAN DPVLSTISK DPVLSTISK_480.27_312.2 480.27 312.2 12.44 63 23.6 PKDRE_HUMAN VIVIPNPVD VIVIPNPVDLR_617.88_312.2 617.88 312.2 20.18 80.7 29.7 LR DDR2 HUMAN YLSSLNFVH YLSSLNFVHR_412.56_312.2 412.56 312.2 16.07 54.2 21.6 R ACBD7_HUMAN ELYGLYK ELYGLYK_443.24_310.2 443.24 310.2 14.58 58.2 19 TAU_HUMAN TDHGAEIVY TDHGAEIVYK_566.79_310.2 566.79 310.2 9.06 74.1 33.4 K ITIH2_HUMAN AHVSFKPTV AHVSFKPTVAQQR_490.27_ 490.27 308.2 9.87 64.2 28.6 AQQR 308.2 GP125_HUMAN SYGLNLAIQ SYGLNLAIQNGPIK_744.41_ 744.41 308.1 18.45 97 38.3 NGPIK 308.1 CALL3_HUMAN ELGTVMR ELGTVMR_403.2_306.1 403.2 306.1 9.7 53 20.2 CB080_HUMAN NATSISAK NATSISAK_396.22_305.2 396.22 305.2 6.49 52.1 28.9 ARP21_HUMAN VAAYFGLDH VAAYFGLDHNVDQTGK_ 578.95 305.2 14.89 75.7 36.1 NVDQTGK 578.95_305.2 GOLM1_HUMAN DLSENNDQR DLSENNDQR_545.74_303.2 545.74 303.2 6.25 71.4 35.5 EMIL3_HUMAN LQHTVGHFDQ LQHTVGHFDQR_446.56_ 446.56 303.2 8.57 58.6 29.3 R 303.2 CACB2_HUMAN TLATSSLPLS TLATSSLPLSPTLASNSQGS 839.42 303.2 16.73 109.3 49.4

PTLASNSQGS QGDQR_839.42_303.2 QGDQR AFAM_HUMAN DADPDTFFAK DADPDTFFAK_563.76_302.1 563.76 302.1 15.58 73.7 24.3 CNTN1_HUMAN DGEYVVEVR DGEYVVEVR_533.26_302.1 533.26 302.1 12.46 69.8 26 CAD19_HUMAN VSDINDNEPK VSDINDNEPK_565.77_302.1 565.77 302.1 7.2 74 30.4 CO4A4_HUMAN GDPGPPGAEG GDPGPPGAEGPPGLPGK_ 750.38 301.2 11.98 97.8 41.5 PPGLPGK 750.38_301.2 PLCB1_HUMAN ISEDSNHGSA ISEDSNHGSAPLSLSSDPGK 666.65 301.2 11.82 87 40.5 PLSLSSDPGK _666.65_301.2 CF072_HUMAN NVETTVDEDV NVETTVDEDVLPGK_758.38 758.38 301.2 14.91 98.8 32.9 LPGK _301.2 GABR2_HUMAN NVSIPALNDS NVSIPALNDSK_579.31_ 579.31 301.2 14.46 75.7 28 K 301.2 SCG1_HUMAN NYLNYGEEGA NYLNYGEEGAPGK_706.33_ 706.33 301.2 12.31 92.1 42.6 PGK 301.2 GP158_HUMAN YELAGLPGK YELAGLPGK_474.26_301.2 474.26 301.2 14.14 62.2 32.4 ELAV3_HUMAN DANLYVSGLP DANLYVSGLPK_588.82_ 588.82 301.1 15.26 77 28.4 K 301.1 KCTD4_HUMAN NGELLLPEGF NGELLLPEGFR_622.84_ 622.84 301.1 19.68 81.3 29.9 R 301.1 A1AG2_HUMAN NWGLSFYADK NWGLSFYADKPETTK_ 586.29 301.1 18.43 76.6 24.5 PETTK 586.29_301.1 CD72_HUMAN NWQESQK NWQESQK_460.22_301.1 460.22 301.1 7.02 60.4 19.7 MAMC2_HUMAN IWSVLESPR IWSVLESPR_543.8_300.2 543.8 300.2 18.18 71.2 23.4 SCRT1_HUMAN LDAFSSADLE LDAFSSADLESAYGR_ 801.38 300.2 17.31 104.4 40.8 SAYGR 801.38_300.2 SGCZ_HUMAN LEGISEFLLP LEGISEFLLPLYVK_810.97_ 810.97 300.2 25.2 105.6 41.2 LYVK 300.2 CAD22_HUMAN LWAAGTPSPS LWAAGTPSPSAPGAR_ 719.88 300.2 12.93 93.9 40.2 APGAR 719.88_300.2 PTPRB_HUMAN SLVVSWSPPA SLVVSWSPPAGDWEQYR_ 988.98 300.2 21.21 128.6 46 GDWEQYR 988.98_300.2 ADA23_HUMAN IGQLQGEIIP IGQLQGEIIPTSFYHQGR_ 682.03 299.2 18.63 89 32.3 TSFYHQGR 682.03_299.2 PCDAA_HUMAN LNASDSDEGI LNASDSDEGINK_631.8_ 631.8 299.2 7.92 82.5 30.3 NK 299.2 GFRA2_HUMAN NAIQAFGNGT NAIQAFGNGTDVNVSPK_ 866.44 299.2 14.98 112.8 40.6 DVNVSPK 866.44_299.2 RUN3A_HUMAN TPVVIDYTPY TPVVIDYTPYLK_704.89_ 704.89 298.2 18.83 91.9 36.5 LK 298.2 CFAH_HUMAN NDFTWFK NDFTWFK_479.23_294.2 479.23 294.2 19.79 62.8 32.6 EMIL3 HUMAN VHGLALGHEA VHGLALGHEAHLQR_769.42 769.42 294.2 11.14 100.3 45.4 HLQR _294.2 MOT2_HUMAN YLDFSLFK YLDFSLFK_516.77_294.2 516.77 294.2 23.12 67.7 34.2 NRG3_HUMAN EYVPTDLVDS EYVPTDLVDSK_633.32_ 633.32 293.1 14.42 82.7 30.4 K 293.1 NFL_HUMAN YEEEVLSR YEEEVLSR_512.75_293 .1 512.75 293.1 10.61 67.1 25.1 CADH5_HUMAN YEIVVEAR YEIVVEAR_489.77_293.1 489.77 293.1 13.14 64.2 24 GP158_HUMAN YELAGLPGK YELAGLPGK_474.26_293.1 474.26 293.1 14.14 62.2 23.4 I12R1_HUMAN YEPPLGDIK YEPPLGDIK_516.27_293.1 516.27 293.1 14.41 67.6 22.2 GPC6_HUMAN GFSLADIPYQ GFSLADIPYQEIAGEHLR_ 672.68 292.1 22.21 87.8 28.9 EIAGEHLR 672.68_292.1 MMP9_HUMAN AFALWSAVTP AFALWSAVTPLTFTR_ 840.96 290.2 25.11 109.5 42.5 LTFTR 840.96_290.2 ABCA1_HUMAN EGAFVELFHE EGAFVELFHEIDDR_559.6_ 559.6 290.1 23.86 73.2 32.1 IDDR 290.1 FETUA_HUMAN AHYDLR AHYDLR_258.8_288.2 258.8 288.2 8.05 34.4 13.7 APOA1_HUMAN AKPALEDLR AKPALEDLR_506.79_288.2 506.79 288.2 11.56 66.4 33.8 CALL3_HUMAN DGNGFVSAAE DGNGFVSAAELR_618.3_ 618.3 288.2 15.13 80.8 35.7 LR 288.2 HRG_HUMAN HSHESQDLR HSHESQDLR_370.18_288.2 370.18 288.2 5.96 48.8 22.4 ZIC1_HUMAN LVNHIR LVNHIR_251.16_288.2 251.16 288.2 7.04 33.4 19.4 APOB_HUMAN SPAFTDLHLR SPAFTDLHLR_386.21_288.2 386.21 288.2 15.75 50.8 26.2 CO2_HUMAN TAVDHIR TAVDHIR_406.22_288.2 406.22 288.2 7.01 53.4 29.4 APOD_HUMAN VLNQELR VLNQELR_436.25_288.2 436.25 288.2 9.77 57.3 30.7 S39A4_HUMAN ALTPGLSWLL ALTPGLSWLLQR_677.9_ 677.9 286.2 24.56 88.4 29.3 QR 286.2 LCK HUMAN ERPEDRPTFD ERPEDRPTFDYLR_565.28_ 565.28 286.2 14.43 73.9 35.4 YLR 286.2 PARK7_HUMAN DGLILTSR DGLILTSR_437.75_286.1 437.75 286.1 13.64 57.5 21.8 EPHA8_HUMAN DLGASTQESQ DLGASTQESQFLK_712.36_ 712.36 286.1 14.58 92.9 36.8 FLK 286.1 PCDB7_HUMAN DLGLGVGELR DLGLGVGELR_514.79_286.1 514.79 286.1 17.2 67.4 28.2 TYRO_HUMAN DLGYDYSYLQ DLGYDYSYLQDSDPDSFQD 883.05 286.1 21.17 114.9 48.6 DSDPDSFQDY YIK_883.05_286.1 IK SGCZ_HUMAN LGNLPTGSFS LGNLPTGSFSSSSPSSSSSR_ 971.46 285.2 13.75 126.3 51.2 SSSPSSSSSR 971.46_285.2 NKX62_HUMAN LNGLASSAGV LNGLASSAGVYFGPAAAVA 946.51 285.2 19.6 123.1 53.1 YFGPAAAAVR R_946.51_285.2 SREC_HUMAN HFGSFQK HFGSFQK_425.71_285.1 425.71 285.1 9.6 55.9 24.2 FA20A_HUMAN HFPTISADYS HFPTISADYSQDEK_546.59_ 546.59 285.1 13.78 71.5 31.4 QDEK 285.1 S15A2_HUMAN FVNTLHK FVNTLHK_429.75_284.2 429.75 284.2 8.42 56.4 30.4 ERMIN_HUMAN LFIVHK LFIVHK_378.74_284.2 378.74 284.2 11.75 49.9 28.2 GP158_HUMAN LLGFATVYGT LLGFATVYGTVTLK_741.93 741.93 284.2 23.26 96.7 38.1 VTLK _284.2 LAT3_HUMAN LSGLALDHK LSGLALDHK_318.52_284.2 318.52 284.2 11.36 42.1 22.8 INHBA_HUMAN GHSPFANLK GHSPFANLK_485.76_282.1 485.76 282.1 12.42 63.7 23.9 CAMKV_HUMAN YDLGQVIK YDLGQVIK_468.26_279.1 468.26 279.1 15.27 61.4 23.1 NETO1_HUMAN YNFTPDPDFK YNFTPDPDFK_622.29_278.1 622.29 278.1 16.1 81.3 29.9 GRM5_HUMAN IYSNAGEQSF IYSNAGEQSFDK_679.82_ 679.82 277.2 10.33 88.7 32.4 DK 277.2 KIF5A_HUMAN LYLVDLAGSE LYLVDLAGSEK_604.33_ 604.33 277.2 18.66 79 29.1 K 277.2 CEL3B_HUMAN LYTNGPLPDK LYTNGPLPDK_559.3_277.2 559.3 277.2 11.76 73.1 24.1 CO8A_HUMAN LYYGDDEK LYYGDDEK_501.72_277.2 501.72 277.2 9.1 65.7 21.6 GPR88_HUMAN YLLITR YLLITR_389.74_277.2 389.74 277.2 14.87 51.3 19.6 ITA3_HUMAN YLLLAGAPR YLLLAGAPR_487.3_277.2 487.3 277.2 17.15 63.9 23.9 VTNC_HUMAN FEDGVLDPDY FEDGVLDPDYPR_711.83_ 711.83 277.1 16.02 92.8 33.8 PR 277.1 PAR6B_HUMAN FEEFYGLLQH FEEFYGLLQHVHK_549.62_ 549.62 277.1 19.68 71.9 25.6 VHK 277.1 KCNA2_HUMAN FETQLK FETQLK_383.21_277.1 383.21 277.1 9.93 50.4 19.4 CBPN_HUMAN IVQLIQDTR IVQLIQDTR_543.32_276.2 543.32 276.2 14.84 71.1 26.4 ASGR1_HUMAN SLESQLEK SLESQLEK_467.25_276.2 467.25 276.2 11.96 61.3 32.1 ALBU_HUMAN TYETTLEK TYETTLEK_492.75_276.2 492.75 276.2 9.43 64.6 33.2 CADH5_HUMAN YTFVVPEDTR YTFVVPEDTR_613.81_276.2 613.81 276.2 15.34 80.2 38.5 NPTX1_HUMAN FQLTFPLR FQLTFPLR_511.3_276.1 511.3 276.1 21.84 67 25 IC1_HUMAN FQPTLLTLPR FQPTLLTLPR_593.35_276.1 593.35 276.1 20.26 77.5 25.6 ZIC1_HUMAN HHSAGDVAER HHSAGDVAER_360.17_ 360.17 275.1 5.9 47.5 24.9 275.1 HAS1_HUMAN VGAVGGDVR VGAVGGDVR_415.23_274.2 415.23 274.2 7.58 54.6 29.8 MMP24_HUMAN AAPGPPPPPP AAPGPPPPPPPPGQAPR_ 534.29 272.2 9.77 69.9 24.8 PPGQAPR 534.29_272.2 CFAB_HUMAN DISEVVTPR DISEVVTPR_339.19_272.2 339.19 272.2 12.19 44.8 17.8 MMP1_HUMAN DIYSSFGFPR DIYSSFGFPR_396.86_272.2 396.86 272.2 20.37 52.2 17.8 SEM4A_HUMAN EAILALDIQD EAILALDIQDPGVPR_803.94 803.94 272.2 20.26 104.7 34.9 PGVPR _272.2 GAS6_HUMAN EVFENDPETD EVFENDPETDYFYPR_ 960.92 272.2 18.37 125 41.8 YFYPR 960.92_272.2 LRMP_HUMAN EYSSLPLPR EYSSLPLPR_531.29_272.2 531.29 272.2 14.85 69.5 25.9 KAIN_HUMAN FSISGSYVLD FSISGSYVLDQILPR_565.64_ 565.64 272.2 24.79 74 23.4 QILPR 272.2 IPSP_HUMAN GFQQLLQELN GFQQLLQELNQPR_524.28_ 524.28 272.2 23.15 68.6 24.3 QPR 272.2 BDNF_HUMAN GLTSLADTFE GLTSLADTFEHVIEELLDED 834.75 272.2 25.61 108.7 37.1 HVIEELLDED QK_834.75_272.2 QK VGFR3_HUMAN HATLSLSIPR HATLSLSIPR_365.55_272.2 365.55 272.2 15.55 48.2 16.2 CSF1R_HUMAN HTFTLSLPR HTFTLSLPR_536.3_272.2 536.3 272.2 16.88 70.2 35.1 KCC2A_HUMAN ITQYLDAGGI ITQYLDAGGIPR_652.35_ 652.35 272.2 14.93 85.2 28.2 PR 272.2 MAMC2_HUMAN IWSVLESPR IWSVLESPR_543.8_272.2 543.8 272.2 18.18 71.2 26.4 GFRA2_HUMAN LASIFSGTGA LASIFSGTGADPVVSAK_ 810.44 272.2 17.97 105.5 41.2 DPVVSAK 810.44_272.2 GT253_HUMAN LDLTGSSGHS LDLTGSSGHSLQPQPR_ 564.96 272.2 12.28 73.9 35.4 LQPQPR 564.96_272.2 GALR3_HUMAN LLAGGGQGPE LLAGGGQGPEPR_576.31_ 576.31 272.2 9.45 75.3 36.9 PR 272.2 WNT8B_HUMAN TLGLLGTEGR TLGLLGTEGR_508.79_272.2 508.79 272.2 15.38 66.6 27.9 OPCM_HUMAN WSIDPR WSIDPR_387.2_272.2 387.2 272.2 13.74 50.9 25.5 KCNV2_HUMAN AQAQVEEAEE AQAQVEEAEELFR_760.37_ 760.37 271.1 18.64 99.1 36 LFR 271.1 FIBB_HUMAN IRPFFPQQ IRPFFPQQ_516.79_270.2 516.79 270.2 16.47 67.7 22.2

DIRA2_HUMAN VAVFGAGGVG VAVFGAGGVGK_481.28_ 481.28 270.2 13.16 63.1 23.7 K 270.2 SYN2_HUMAN TPALSPQRPL TPALSPQRPLTTQQPQSGTL 750.41 270.1 12.34 97.8 38.8 TTQLKQPQSG K_750.41_270.1 T APOC2_HUMAN TYLPAVDEK TYLPAVDEK_518.27_265.1 518.27 265.1 12.36 67.9 25.3 CRUM1_HUMAN EPEFLNISIQ EPEFLNISIQDSR_774.39_ 774.39 262.2 19.34 100.9 42.6 DSR 262.2 UGGG2_HUMAN INEENTAISR INEENTAISR_573.79_262.2 573.79 262.2 8.3 75 27.7 ARHG7_HUMAN FNFQQTNEDE FNFQQTNEDELSFSK_917.42 917.42 262.1 17.52 119.3 48.9 LSFSK _262.1 NEUM_HUMAN IEQDGIKPED IEQDGIKPEDK_636.33_262.1 636.33 262.1 7.45 83.1 36.5 K GP113_HUMAN NLLIATDK NLLIATDK_444.26_262.1 444.26 262.1 13.39 58.3 31 GELS_HUMAN AQPVQVAEGS AQPVQVAEGSEPDGFWEAL 758.03 261.2 21.24 98.8 36.2 EPDGFWEALG GGK_758.03_261.2 GK SPB5_HUMAN DVPFGFQTVT DVPFGFQTVTSDVNK_ 827.41 261.2 18.74 107.7 47.9 SDVNK 827.41_261.2 ERLN1_HUMAN EALEPSGENV EALEPSGENVIQNK_764.39_ 764.39 261.2 11.75 99.6 36.1 IQNK 261.2 PDYN_HUMAN FLPSISTK FLPSISTK_446.76_261.2 446.76 261.2 13.97 58.6 19.2 CADH7_HUMAN FLSLGPFSDT FLSLGPFSDTTTVK_756.9_ 756.9 261.2 21.28 98.6 35.8 TTVK 261.2 APC2_HUMAN APATASATLL APATASATLLE_522.78_ 522.78 261.1 14.37 68.4 22.5 E 261.1 LYG2_HUMAN AIKPYQTLIK AIKPYQTLIK_392.25_260.2 392.25 260.2 13.84 51.6 26.6 ACBD7_HUMAN ARPDDGELK ARPDDGELK_500.76_260.2 500.76 260.2 6.72 65.6 27.5 KCNA2_HUMAN EDEGYIK EDEGYIK_427.2_260.2 427.2 260.2 8.47 56.1 21.3 C1QL2_HUMAN SPHEGYEVLK SPHEGYEVLK_386.86_260.2 386.86 260.2 10.69 50.9 23.3 SEM4A_HUMAN TLGSQPVLK TLGSQPVLK_471.78_260.2 471.78 260.2 10.96 61.9 32.3 I12R1_HUMAN YEPPLGDIK YEPPLGDIK_516.27_260.2 516.27 260.2 14.41 67.6 28.2 AMNLS_HUMAN SGDEAPGLFF SGDEAPGLFFVDAER_ 805.38 260.1 20.88 104.9 43.9 RVDAE 805.38_260.1 ADA23_HUMAN AWGAAAPSAP AWGAAAPSAPHWNETAEK 631.97 258.1 14.4 82.5 26.8 HWNEKTAE _631.97_258.1 SCG1_HUMAN GEAGAPGEED GEAGAPGEEDIQGPTK_ 778.37 258.1 9.56 101.4 39.7 IQGPTK 778.37_258.1 SNTB1_HUMAN SGLLEVLVR SGLLEVLVR_493.31_258.1 493.31 258.1 21.74 64.6 24.2 GPC5_HUMAN NAAAFQETLE NAAAFQETLETLIK_774.92_ 774.92 257.1 24.18 101 36.6 TLIK 257.1 SHSA7_HUMAN SPALPPDPTA SPALPPDPTAR_561.3_256.1 561.3 256.1 10.3 73.4 33.2 R ADA12_HUMAN NHPEVLNIR NHPEVLNIR_546.3_252.1 546.3 252.1 12.38 71.5 29.5 ITIH3_HUMAN NHQLIVTR NHQLIVTR_490.79_252.1 490.79 252.1 9.24 64.3 30.1 RCN1_HUMAN HLVYESDK HLVYESDK_495.75_251.2 495.75 251.2 7.73 65 30.3 CBPN_HUMAN HLYVLEFSDH HLYVLEFSDHPGIHEPLEPE 862.44 251.2 19.33 112.3 44.5 PGIHEPLEPE VK_862.44_251.2 VK HS3S5_HUMAN IHPEVDPSVI IHPEVDPSVITK_445.58_ 445.58 251.2 12.75 58.5 29.3 TK 251.2 CO3_HUMAN IHWESASLLR IHWESASLLR_404.56_251.2 404.56 251.2 17.03 53.2 27.2

IV. Reduced Set of Exemplary Protein Results (Table II)

TABLE-US-00002 [0069] Lengthy table referenced here US20180284128A1-20181004-T00001 Please refer to the end of the specification for access instructions.

V. Exemplary Protein/Peptide Selection Results

TABLE-US-00003 [0070] Lengthy table referenced here US20180284128A1-20181004-T00002 Please refer to the end of the specification for access instructions.

VI. Exemplary Assayed Protein

[0071] The expression of the following 357 proteins were assessed substantially simultaneously using the MS-LC-SRM-MS system described herein.

[0072] 41_HUMAN

[0073] 5HT2C_HUMAN

[0074] A1AG1_HUMAN

[0075] A1AG2_HUMAN

[0076] A1BG_HUMAN

[0077] A2MG_HUMAN

[0078] A4_HUMAN

[0079] AACT_HUMAN

[0080] ABCA1_HUMAN

[0081] ACBD7_HUMAN

[0082] ACE2_HUMAN

[0083] ACHA2_HUMAN

[0084] ACHA4_HUMAN

[0085] ACHA5_HUMAN

[0086] ACHB_HUMAN

[0087] ACHB2_HUMAN

[0088] ADA12_HUMAN

[0089] ADA23_HUMAN

[0090] AFAM_HUMAN

[0091] AGAL_HUMAN

[0092] AGAP2_HUMAN

[0093] ALBU_HUMAN

[0094] ALS_HUMAN

[0095] AMFR2_HUMAN

[0096] AMNLS_HUMAN

[0097] AMPB_HUMAN

[0098] ANGP1_HUMAN

[0099] ANGT_HUMAN

[0100] ANO3_HUMAN

[0101] ANT3_HUMAN

[0102] AP1B1_HUMAN

[0103] APC2_HUMAN

[0104] APLP1_HUMAN

[0105] APOA1_HUMAN

[0106] APOA2_HUMAN

[0107] APOA4_HUMAN

[0108] APOB_HUMAN

[0109] APOC2_HUMAN

[0110] APOD_HUMAN

[0111] APOE_HUMAN

[0112] APOL4_HUMAN

[0113] APOOL_HUMAN

[0114] ARHG7_HUMAN

[0115] ARP21_HUMAN

[0116] ARSA_HUMAN

[0117] ARSE_HUMAN

[0118] ARTN_HUMAN

[0119] ASGR1_HUMAN

[0120] AT12A_HUMAN

[0121] AT2A2_HUMAN

[0122] AT2B3_HUMAN

[0123] ATS1_HUMAN

[0124] BAX_HUMAN

[0125] BCAS1_HUMAN

[0126] BDNF_HUMAN

[0127] BEST1_HUMAN

[0128] BTNL8_HUMAN

[0129] C1QL2_HUMAN

[0130] C1QT4_HUMAN

[0131] CACB2_HUMAN

[0132] CAD11_HUMAN

[0133] CAD19_HUMAN

[0134] CAD22_HUMAN

[0135] CADH3_HUMAN

[0136] CADH5_HUMAN

[0137] CADH7_HUMAN

[0138] CALL3_HUMAN

[0139] CAMKV_HUMAN

[0140] CAR14_HUMAN

[0141] CATD_HUMAN

[0142] CB080_HUMAN

[0143] CB085_HUMAN

[0144] CBPN_HUMAN

[0145] CD3D_HUMAN

[0146] CD72_HUMAN

[0147] CEL3A_HUMAN

[0148] CEL3B_HUMAN

[0149] CERU_HUMAN

[0150] CETP_HUMAN

[0151] CF072_HUMAN

[0152] CFAB_HUMAN

[0153] CFAH_HUMAN

[0154] CHAD_HUMAN

[0155] CK041_HUMAN

[0156] CLC4M_HUMAN

[0157] CLUS_HUMAN

[0158] CNTN1_HUMAN

[0159] CNTN2_HUMAN

[0160] CO1A2_HUMAN

[0161] CO2_HUMAN

[0162] CO3_HUMAN

[0163] CO4A_HUMAN

[0164] CO4A4_HUMAN

[0165] CO4B_HUMAN

[0166] CO6_HUMAN

[0167] CO8A_HUMAN

[0168] CO9A2_HUMAN

[0169] COIA1_HUMAN

[0170] CORT_HUMAN

[0171] CP46A_HUMAN

[0172] CPLX2_HUMAN

[0173] CRLF1_HUMAN

[0174] CRUM1_HUMAN

[0175] CSF1_HUMAN

[0176] CSF1R_HUMAN

[0177] DBC1_HUMAN

[0178] DCBD1_HUMAN

[0179] DCBD2_HUMAN

[0180] DDR2_HUMAN

[0181] DIRA2_HUMAN

[0182] E41L3_HUMAN

[0183] EAA2_HUMAN

[0184] EDNRB_HUMAN

[0185] ELAV3_HUMAN

[0186] EMIL2_HUMAN

[0187] EMIL3_HUMAN

[0188] EPHA8_HUMAN

[0189] ERLN1_HUMAN

[0190] ERMIN_HUMAN

[0191] ERO1B_HUMAN

[0192] F123A_HUMAN

[0193] F13A_HUMAN

[0194] FA20A_HUMAN

[0195] FCGRN_HUMAN

[0196] FETUA_HUMAN

[0197] FEZ1_HUMAN

[0198] FGFR2_HUMAN

[0199] FGFR3_HUMAN

[0200] FGL1_HUMAN

[0201] FIBA_HUMAN

[0202] FIBB_HUMAN

[0203] FIBG_HUMAN

[0204] FINC_HUMAN

[0205] FRS2_HUMAN

[0206] GABR2_HUMAN

[0207] GALR3_HUMAN

[0208] GAS6_HUMAN

[0209] GBRA2_HUMAN

[0210] GBRB2_HUMAN

[0211] GELS_HUMAN

[0212] GFRA2_HUMAN

[0213] GNAQ_HUMAN

[0214] GOLM1_HUMAN

[0215] GOPC_HUMAN

[0216] GP113_HUMAN

[0217] GP125_HUMAN

[0218] GP158_HUMAN

[0219] GP2_HUMAN

[0220] GPC5_HUMAN

[0221] GPC5D_HUMAN

[0222] GPC6_HUMAN

[0223] GPR88_HUMAN

[0224] GRIA2_HUMAN

[0225] GRM5_HUMAN

[0226] GRN_HUMAN

[0227] GT253_HUMAN

[0228] HAS1_HUMAN

[0229] HCN1_HUMAN

[0230] HCN2_HUMAN

[0231] HEMO_HUMAN

[0232] HEP2_HUMAN

[0233] HPCA_HUMAN

[0234] HPT_HUMAN

[0235] HRG_HUMAN

[0236] HS3 S5_HUMAN

[0237] I12R1_HUMAN

[0238] IC1_HUMAN

[0239] ICAM3_HUMAN

[0240] IGF1R_HUMAN

[0241] IL12B_HUMAN

[0242] IL1AP_HUMAN

[0243] IL1R2_HUMAN

[0244] INADL_HUMAN

[0245] INHBA_HUMAN

[0246] IPSP_HUMAN

[0247] ITA3_HUMAN

[0248] ITAM_HUMAN

[0249] ITB2_HUMAN

[0250] ITB5_HUMAN

[0251] ITIH1_HUMAN

[0252] ITIH2_HUMAN

[0253] ITIH3_HUMAN

[0254] ITIH4_HUMAN

[0255] JPH3_HUMAN

[0256] KAIN_HUMAN

[0257] KALRN_HUMAN

[0258] KCC1G_HUMAN

[0259] KCC2A_HUMAN

[0260] KCNA1_HUMAN

[0261] KCNA2_HUMAN

[0262] KCNA3_HUMAN

[0263] KCNA5_HUMAN

[0264] KCNQ1_HUMAN

[0265] KCNV2_HUMAN

[0266] KCTD4_HUMAN

[0267] KIF5A_HUMAN

[0268] KIRR2_HUMAN

[0269] KLK3_HUMAN

[0270] KLKB1_HUMAN

[0271] KNG1_HUMAN

[0272] KSYK_HUMAN

[0273] LAMB2_HUMAN

[0274] LAT2_HUMAN

[0275] LAT3_HUMAN

[0276] LCK_HUMAN

[0277] LCN8_HUMAN

[0278] LGI1_HUMAN

[0279] LGMN_HUMAN

[0280] LIPE_HUMAN

[0281] LRMP_HUMAN

[0282] LRP8_HUMAN

[0283] LRTM2_HUMAN

[0284] LSHR_HUMAN

[0285] LTBP1_HUMAN

[0286] LYG2_HUMAN

[0287] MAMC2_HUMAN

[0288] MAP4_HUMAN

[0289] MICA_HUMAN

[0290] MMP1_HUMAN

[0291] MMP16_HUMAN

[0292] MMP17_HUMAN

[0293] MMP20_HUMAN

[0294] MMP24_HUMAN

[0295] MMP9_HUMAN

[0296] MOT2_HUMAN

[0297] MPDZ_HUMAN

[0298] MTOR_HUMAN

[0299] MYP2_HUMAN

[0300] NCAN_HUMAN

[0301] NCKX2_HUMAN

[0302] NDF6_HUMAN

[0303] NECP2_HUMAN

[0304] NETO1_HUMAN

[0305] NETR_HUMAN

[0306] NEUG_HUMAN

[0307] NEUM_HUMAN

[0308] NFL_HUMAN

[0309] NKX62_HUMAN

[0310] NMDE1_HUMAN

[0311] NMDE3_HUMAN

[0312] NMDZ1_HUMAN

[0313] NMS_HUMAN

[0314] NOE3_HUMAN

[0315] NPT4_HUMAN

[0316] NPTX1_HUMAN

[0317] NRG3_HUMAN

[0318] NTRK2_HUMAN

[0319] ODP2_HUMAN

[0320] OLFL3_HUMAN

[0321] OLIG1_HUMAN

[0322] OPCM_HUMAN

[0323] OTOAN_HUMAN

[0324] P2RX1_HUMAN

[0325] P4K2A_HUMAN

[0326] PACN1_HUMAN

[0327] PAK3_HUMAN

[0328] PAQR6_HUMAN

[0329] PAR6B_HUMAN

[0330] PARD3_HUMAN

[0331] PARK7_HUMAN

[0332] PCD18_HUMAN

[0333] PCDA5_HUMAN

[0334] PCDAA_HUMAN

[0335] PCDB6_HUMAN

[0336] PCDB7_HUMAN

[0337] PCDBC_HUMAN

[0338] PCDBF_HUMAN

[0339] PCDGE_HUMAN

[0340] PCDGF_HUMAN

[0341] PCSK1_HUMAN

[0342] PDIA2_HUMAN

[0343] PDYN_HUMAN

[0344] PEDF_HUMAN

[0345] PERL_HUMAN

[0346] PGCB_HUMAN

[0347] PGCP_HUMAN

[0348] PICAL_HUMAN

[0349] PIN1_HUMAN

[0350] PKDRE_HUMAN

[0351] PLCB1_HUMAN

[0352] PON1_HUMAN

[0353] PRIO_HUMAN

[0354] PSMG1_HUMAN

[0355] PTN5_HUMAN

[0356] PTPRB_HUMAN

[0357] PTPRO_HUMAN

[0358] PTPRT_HUMAN

[0359] PVRL1_HUMAN

[0360] PZP_HUMAN

[0361] RCN1_HUMAN

[0362] RELN_HUMAN

[0363] RES18_HUMAN

[0364] RGS11_HUMAN

[0365] RGS20_HUMAN

[0366] RGS4_HUMAN

[0367] RRAGC_HUMAN

[0368] RUN3A_HUMAN

[0369] S12A5_HUMAN

[0370] S12A6_HUMAN

[0371] S15A2_HUMAN

[0372] S39A4_HUMAN

[0373] SAA4_HUMAN

[0374] SCG1_HUMAN

[0375] SCG3_HUMAN

[0376] SCN2A_HUMAN

[0377] SCNNA_HUMAN

[0378] SCRT1_HUMAN

[0379] SEM4A_HUMAN

[0380] SEMG1_HUMAN

[0381] SEPP1_HUMAN

[0382] SEPT3_HUMAN

[0383] SGCZ_HUMAN

[0384] SHSA7_HUMAN

[0385] SIA8C_HUMAN

[0386] SIG12_HUMAN

[0387] SIX3_HUMAN

[0388] SLIK1_HUMAN

[0389] SLIT1_HUMAN

[0390] SNP25_HUMAN

[0391] SNTB1_HUMAN

[0392] SO1A2_HUMAN

[0393] SO1B3_HUMAN

[0394] SPB5_HUMAN

[0395] SREC_HUMAN

[0396] STH_HUMAN

[0397] SYN2_HUMAN

[0398] SYNPR_HUMAN

[0399] SYTL4_HUMAN

[0400] SYUA_HUMAN

[0401] SYUB_HUMAN

[0402] T151A_HUMAN

[0403] TADBP_HUMAN

[0404] TAU_HUMAN

[0405] TBB2B_HUMAN

[0406] TERA_HUMAN

[0407] TFR2_HUMAN

[0408] TLR7_HUMAN

[0409] TM9S1_HUMAN

[0410] TMPS2_HUMAN

[0411] TNF6B_HUMAN

[0412] TNR19_HUMAN

[0413] TR11B_HUMAN

[0414] TRFR_HUMAN

[0415] TRIM9_HUMAN

[0416] TRPV5_HUMAN

[0417] TYRO_HUMAN

[0418] UGGG2_HUMAN

[0419] UNC5 C_HUMAN

[0420] VGFR3_HUMAN

[0421] VTDB_HUMAN

[0422] VTNC_HUMAN

[0423] WNK4_HUMAN

[0424] WNT8B_HUMAN

[0425] XLRS1_HUMAN

[0426] YQ051_HUMAN

[0427] ZIC1_HUMAN

[0428] ZIC2_HUMAN

TABLE-US-LTS-00001 LENGTHY TABLES The patent application contains a lengthy table section. A copy of the table is available in electronic form from the USPTO web site (http://seqdata.uspto.gov/?pageRequest=docDetail&DocID=US20180284128A1). An electronic copy of the table will also be available from the USPTO upon request and payment of the fee set forth in 37 CFR 1.19(b)(3).

Claims

1. A multiplexed LC-SRM-MS assay for the measurement of a plurality of proteins in a single sample comprising: a) generating a set of optimal peptides and corresponding transitions for each protein monitored; b) optimizing the collision energy for each transition such that interference among the transitions monitored is avoided; c) selecting a set of transitions that have the greatest peak areas are monitored for each of the proteins, and wherein the selected transitions do not interfere with the ions in the sample; d) monitoring the detected set of transitions for each protein in the sample, thereby measuring a plurality of proteins in the sample.

2. The assay of claim 1, wherein each monitored peptide (i) has a monoisotopic mass of 700-5000 Da; and (ii) does not contain a cysteine or a methionine; and.

3. The assay of claim 1, wherein the transitions for each peptide (i) have one of the four most intense b or y transition ions; (ii) has m/z values of at least 30 m/z above or below those of a precursor ion; (iii) do not interfere with transitions from other peptides; and (iv) represent transitions due to breakage of peptide bond at different sites of the protein.

4. The assay according to claim 1, wherein the peptides do not include any peptide that is bounded by KK, KR, RK or RR, either upstream of downstream in the corresponding protein sequence.

5. The assay according to claim 1, wherein each peptide of said set of peptides is unique to the corresponding protein.

6. The assay according to claim 1, wherein the peptides do not include peptides which were observed in post-translational modified forms.

7. The assay according to claim 1, wherein each set of peptides is prioritized according to one or more of the following ordered set of criteria: (a) unique peptides first, then non-unique; (b) peptides with no observed post-translational modifications first, then those observed with post-translational modifications; (c) peptides within the mass range 800-3500 Da first, then those outside of 800-3500 Da; and (d) sorted by decreasing number of variant residues.

8. The assay according to claim 7, wherein each set of peptides is prioritized according to all of the ordered set of criteria.

9. The assay according to any one of claims 7 or 8, wherein each prioritized set of peptides contains 1-5 peptides.

10. The assay according to any one of claims 1-9, wherein the two best peptides per protein and the two best transitions per peptide are selected based on experimental data resulting from LC-SRM-MS analysis of one or more of the following experimental samples: a biological disease sample, a biological control sample, and a mixture of synthetic peptides of interest.

11. The assay according to claim 10, wherein the biological disease and biological control samples are processed using an immunodepletion method prior to LC-SRM-MS analysis.

12. The assay according to claim 11, wherein the experimental samples contain internal standard peptides.

13. The assay according to claim 11, wherein the LC-SRM-MS analysis method specifies a maximum of 7000 transitions, including transitions of the internal standard peptides and transitions.

14. The assay according to claim 1, wherein the top two transitions per peptide are selected according to one or more of the following criteria: (1) the transitions exhibit the largest peak areas measured in either of the two biological experimental samples; (2) the transitions are not interfered with by other ions; (3) the transitions do not exhibit an elution profile that visually differs from those of other transitions of the same peptide; (4) the transitions are not beyond the detection limit of both of the two biological experimental samples; and (5) the transitions do not exhibit interferences.

15. The assay according to claim 1, wherein the top two peptides per protein are selected according to one or more of the following criteria: (1) one or more peptides exhibit two transitions according to claim 12 and represent the largest combined peak areas of the two transitions according to claims 12; and (2) one or more peptides exhibit one transition according to claim 12 and represent the largest combined peak areas of the two transitions according to claim 12.

16. An assay developed according to the method of claim 1.