U.S. patent application number 15/861384 was filed with the patent office on 2018-08-23 for staphylococcal antigens.
This patent application is currently assigned to THE UNIVERSITY OF EDINBURGH. The applicant listed for this patent is JEANETTE BANNOEHR, NOURI L. BEN ZAKOUR, J. Ross FITZGERALD. Invention is credited to JEANETTE BANNOEHR, NOURI L. BEN ZAKOUR, J. Ross FITZGERALD.
Application Number | 20180237480 15/861384 |
Document ID | / |
Family ID | 43567607 |
Filed Date | 2018-08-23 |
United States Patent
Application |
20180237480 |
Kind Code |
A1 |
BANNOEHR; JEANETTE ; et
al. |
August 23, 2018 |
STAPHYLOCOCCAL ANTIGENS
Abstract
The present invention provides novel sequences encoding
Staphylococcus pseudintermedius proteins/nucleic acids potentially
useful in the treatment and/or prevention of canine disorders. In
particular, the various protein and/or nucleic acid sequences
described herein may find application as vaccines for use in
treating and/or preventing a variety of canine diseases and/or
conditions caused or contributed to by Staphylococcus
pseudintermedius.
Inventors: |
BANNOEHR; JEANETTE;
(EDINBURGH, GB) ; FITZGERALD; J. Ross; (EDINBURGH,
GB) ; BEN ZAKOUR; NOURI L.; (BRISBANE, AU) |
|
Applicant: |
Name |
City |
State |
Country |
Type |
BANNOEHR; JEANETTE
FITZGERALD; J. Ross
BEN ZAKOUR; NOURI L. |
EDINBURGH
EDINBURGH
BRISBANE |
|
GB
GB
AU |
|
|
Assignee: |
THE UNIVERSITY OF EDINBURGH
Edinburgh
GB
|
Family ID: |
43567607 |
Appl. No.: |
15/861384 |
Filed: |
January 3, 2018 |
Related U.S. Patent Documents
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Application
Number |
Filing Date |
Patent Number |
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13446958 |
Apr 13, 2012 |
9879054 |
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15861384 |
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PCT/GB2010/001916 |
Oct 15, 2010 |
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13446958 |
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61252026 |
Oct 15, 2009 |
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Current U.S.
Class: |
1/1 |
Current CPC
Class: |
C07K 14/31 20130101;
A61K 39/00 20130101; A61K 39/085 20130101; G01N 33/56938 20130101;
A61K 2039/552 20130101; A61P 31/04 20180101 |
International
Class: |
C07K 14/31 20060101
C07K014/31; A61K 39/085 20060101 A61K039/085; G01N 33/569 20060101
G01N033/569 |
Claims
1. A Staphylococcus pseudintermedius protein or nucleic acid
comprising a sequence at least 65% homologous or identical to SEQ
ID NOS: 1-38 or fragments, portions, mutants, derivatives and/or
homologoues/orthologues thereof.
2. The Staphylococcus pseudintermedius protein or nucleic acid of
claim 1, comprising a sequence at least 65% homologous or identical
to (i) the nucleic acid of SEQ ID NO: 5 or a fragment thereof or
(ii) the amino acid sequence of SEQ ID NO: 6 or an antigenic
fragment thereof, for use in raising an immune response in a
subject.
3. The Staphylococcus pseudintermedius protein of claim 2,
comprising an antigenic fragment of the amino acid sequence of SEQ
ID NO: 6, wherein said fragment comprises SEQ ID NO: 37.
4. The Staphylococcus pseudintermedius nucleic acid of claim 2,
comprising a fragment of the nucleic acid, wherein said fragment
comprises SEQ ID NO: 38.
5. The Staphylococcus pseudintermedius protein of claim 1, wherein
the protein is a cell-wall anchored (CWA) or microbial surface
components recognising adhesive matrix molecule (MSCRAMM).
6. The Staphylococcus pseudintermedius protein of claim 1,
comprising a replicable expression vector and nucleic acid.
7. A host cell transformed with the nucleic acid of claim 6.
8. The Staphylococcus pseudintermedius protein of claim 1,
comprising an amino acid sequence having at least 80% identity to
SEQ ID NO: 5.
9. A Staphylococcus pseudintermedius protein comprising an amino
acid sequence having at least 80% identity to SEQ ID NO: 37.
10. The Staphylococcus pseudintermedius protein of claim 9,
comprising an amino acid sequence having at least 90% identity to
SEQ ID NO: 37.
11. A method of raising an immune response to Staphylococcus
pseudintermedius in a canine, said method comprising immunising
said canine with, or administering said canine a Staphylococcus
pseudintermedius protein according to claim 1.
12. An immunogenic composition comprising the protein of claim 1
and a pharmaceutically acceptable carrier.
13. The immunogenic composition of claim 12, further comprising one
or more adjuvant(s) and/or antigens for use in treating or
preventing other diseases and/or conditions.
14. A method of treating or preventing a Staphylococcus
pseudintermedius infection or disease in a canine, said method
comprising administering to the canine the immunogenic composition
of claim 12.
15. A method of treating or preventing canine pyoderma comprising
administering to a dog the immunogenic composition of claim 12.
16. A process for the recombinant production of a Staphylococcus
pseudintermedius protein of claim 1, said process comprising the
steps of: (a) transforming a host cell with the nucleic acid of
claim 1; (b) culturing the cells obtained in (a) under conditions
in which expression of a peptide/protein encoded by the nucleic
acid occurs; and (c) isolating the expressed peptide/protein from
the cell culture or/and a culture supernatant derived
therefrom.
17. An antibody that selectively and/or specifically binds to the
Staphylococcus pseudintermedius protein of claim 1.
18. An antibody that selectively and/or specifically binds to the
Staphylococcus pseudintermedius protein of claim 9.
19. A method of diagnosing an S. pseudintermedius infection, said
method comprising the steps of taking a sample from a canine,
identifying in the sample a level of a protein or nucleic acid of
claim 1, wherein the subject is diagnosed with S. pseudintermedius
infection by identification of the protein, peptide or nucleic in
the sample.
20. A kit for diagnosing, detecting and/or evaluating possible S.
pseudintermedius infections, diseases and/or conditions, said kit
comprising one or more components selected from the group
consisting of: (i) a substrate having the Staphylococcus
pseudintermedius protein of claim 1 bound thereto; (ii) antibodies
which exhibit specificity and/or selectivity for the protein; (iii)
and instructions for diagnosing, detecting and/or evaluating
possible S. pseudintermedius infections, diseases and/or
conditions.
Description
CROSS-REFERENCE TO RELATED APPLICATIONS
[0001] This application is a divisional of U.S. patent application
Ser. No. 13/446,958, filed Apr. 13, 2012, which is a
continuation-in-part of PCT/GB2010/001916, filed Oct. 15, 2010,
which claims the benefit under 35 U.S.C. .sctn. 119(e) to U.S.
Provisional Application No. 61/252,026, filed Oct. 15, 2009, each
of which are hereby incorporated by reference in their
entirety.
FIELD OF THE INVENTION
[0002] The present invention provides novel staphylococcal cell
wall associated proteins, genes encoding the same and vaccines for
use in treating/preventing Staphylococcal infections.
BACKGROUND OF THE INVENTION
[0003] Skin diseases are a major cause of morbidity in dogs and an
important animal welfare issue (Hill et al, 2006). In particular,
superficial bacterial folliculitis (pyoderma) caused by
Staphylococcus pseudintermedius (formerly known as Staphylococcus
intermedius) is one of the most common diseases seen in small
animal veterinary practice, worldwide (Hill et al, 2006).
Superficial pyoderma is characterized by the formation of
follicular pustules and is often associated with pruritus,
alopecia, erythema and swelling. This may develop into deep
pyoderma which typically includes pain, crusting, odor, and
exudation of blood and pus. The disease often occurs as a secondary
infection in dogs with atopic dermatitis (AD) resulting from a type
I hypersensitivity reaction (IgE antibody-associated) to
environmental allergens (Hill et al, 2006). Treatment of canine
pyoderma is often difficult without resorting to aggressive,
medium-term administration of systemic antibacterial agents to
prevent relapse of infection, and such therapy predisposes to the
development of bacterial resistance that may be transferred to
bacteria infecting humans (Guardabassi et al, 2004). Worringly,
methicillin-resistant S. pseudintermedius has recently emerged as a
major problem in veterinary clinics worldwide (Bannoehr et al,
2007). Although rare, several episodes of life-threatening
infections of humans by S. pseudintermedius have been reported with
the typical route of transmission being through dog bite wounds
(Bannoehr et al, 2007). Previously, crude vaccine preparations
based on Staphylococcus aureus phage lysate or S. pseudintermedius
autogenous bacterin have shown promise as adjunctive therapies for
treatment of pyoderma (Curtis et al, 2006), and a
rationally-designed effective vaccine would be a highly desireable
means to reducing or eliminating the suffering associated with the
disease.
[0004] Accordingly, the present invention aims to obviate one or
more of the problems associated with the prior art.
SUMMARY OF THE INVENTION
[0005] The present invention is based upon the identification of
novel gene sequences encoding proteins potentially useful in the
treatment and/or prevention of canine disorders. In particular, the
proteins encoded by the genes described herein, may find
application in the treatment and/or prevention of diseases caused
or contributed to by the bacterial pathogen Staphylococcus
pseudintermedius.
[0006] The inventors have identified a number of Staphylococcus
pseudintermedius genes encoding proteins which may broadly be
classed as members of the cell-wall anchored (CWA) family of
proteins. In certain embodiments, these CWA proteins may be further
grouped as surface proteins known as Microbial Surface Components
Recognising Adhesive Matrix Molecules (MSCRAMM). It should be
understood that while a number microbial organisms may be known to
express MSCRAMM type proteins, the term "MSCRAMM" describes the
phenotypic function of a wide range of diverse surface-associated
proteins of Gram-positive bacteria. As such, while MSCRAMM proteins
may all possess cell-wall anchor motifs and signal sequences for
cell wall transportation, the proteins belonging to this group may
otherwise be structurally diverse. Furthermore, bacterial species
within a particular genus, for example the genus Staphylococcus,
may possess unique MSCRAMM profiles.
[0007] In view of the above, the present invention relates to a
group of surface expressed proteins derived from Staphylococcus
pseudintermedius that may be referred to either as CWA or MSCRAMM
proteins.
[0008] As such, a first aspect of this invention provides an
isolated and/or substantially purified Staphylococcus
pseudintermedius CWA or MSCRAMM nucleic acid or protein sequence
comprising a nucleic acid or amino acid sequence homologous or
identical to any one of the nucleic acid or amino acid sequences
provided as SEQ ID NOS: 1-38 below.
TABLE-US-00001 SEQ ID NO: 1
atggaaaacaaaaacttttttagtattcgtaaactatctattggtgtagg
ttcttgcttaatcgcgagttctttacttgtaaacacgccaagttttgctg
aagaaacagataatgcgaacattaatgacgcacaacaaaacgccttttat
gaaattttacatttgccaaacttaactgaagagcaacaaaatggattcat
ccaaagtcttaaagatgatccaagtgtgagcaacgacattttagtagaag
ctaagaaattaaatgacactcaagctaaacctgattacagtgaagcacaa
caaaatgcattttatgaaattttacatttgtcaaacttaactgaagagca
acaaaatggattcatccaaagtcttaaagatgatccaagtgtgagcaacg
acattttagtagaagctaagaagttaaatgacactcaagctaaacctgat
tacagtgaagcacaacaaaatgcattttatgaaattttacatttgtcaaa
cttaactgaagagcaacaaaatgggttcatccaaagccttaaagatgatc
caagtgtaagtaaagaaattttagcagaagctaagaagttaaatgatagt
caagcacctaaagttgataaagctaaaaaaactgacaaagctgaagcgaa
agcagatgataaagctaaaggtgaagaagccaaaaaatctgaagacaaaa
aagatagcaaagcagataaggcaaaatcgaaaaacgctacacatgttgtt
aaacctggtgaaactttagataatattgctaaagatcatcatacaactgt
tgataaaattgctaaagataacaaaataaaagataaaaatgtgattaaac
taggtcaaaaacttgttgttgataaacaaaaagcaactcaaggaaaacaa
gaagctgtagcgaaaaatgaagtgaaggctttacctaatactggtgaaaa
tgatgatatcgcattattcagcacaacagttgcgggtggcgtaagtatcg
ctttaggttcattattattaggaagaaacagaaaaactagctaa
The protein sequence translated from SEQ ID NO 1 is designated SEQ
ID NO: 2 and is shown below:
TABLE-US-00002 SEQ ID NO: 2
MENKNFFSIRKLSIGVGSCLIASSLLVNTPSFAEETDNANINDAQQNAFY
EILHLPNLTEEQQNGFIQSLKDDPSVSNDILVEAKKLNDTQAKPDYSEAQ
QNAFYEILHLSNLTEEQQNGFIQSLKDDPSVSNDILVEAKKLNDTQAKPD
YSEAQQNAFYEILHLSNLTEEQQNGFIQSLKDDPSVSKEILAEAKKLNDS
QAPKVDKAKKTDKAEAKADDKAKGEEAKKSEDKKDSKADKAKSKNATHVV
KPGETLDNIAKDHHTTVDKIAKDNKIKDKNVIKLGQKLVVDKQKATQGKQ
EAVAKNEVKALPNTGENDDIALFSTTVAGGVSIALGSLLLGRNRKTS SEQ ID NO: 3
atggaaaacaaaaactttttcagcattcgtaaattatcaattggggtggg
ttcatgtttaatcgcgagctctttacttgtgaatacaccaagtttcgcag
aagaaggagataataacgcagaagcgcaacaaaacgctttctctgaggta
gtaaaattacctaaccttagcgaagaacaacgtaatggtttcattcaaag
ccttaaagatgatccaagtacaagtcaagatgtgcttaatgaagctaaaa
aattaaatgatagtcaagagggatctcaacctgctcctgattacagtgat
gaacaacaaaatgcattttatgaaattttacaccttccaaacttaactga
agaacaacgcaatggctatattcaaagtcttaaagatgacccaagtgtaa
gcgctaatattcttgttgaagctaaaaatatgaatgttaaccaaacacct
acacaacctgcgccaagtttcgatgaagcgcaacaaaatgcattctatga
gattgtaaacttaccaaatcttactgaagagcaacgtaacggtttcatcc
aaagccttaaagacgatccaagtgtaagtaaagatatccttgttgaagct
aaaaagttaaatgacagccaagcaaaacctgattacagtgaagcgcaaca
aaatgcattttatgaaattttacaccttccaaacttaactgaagaacaac
gtaacggtttcatccaaagccttaaagacgatccgagtgtaagtagtgat
attcttgctgaagctaagaaattaaatgacagccaagcgcctaaagaaga
caacaacgtaaaagacaataattcaggtgaaaacaaagctgaagacaaag
gcaacaaagaaaacaaagctgaagataaaggcagcaaagaagacaaagct
gaagataaaggcagcaaagaagacaaagctgaagataaaggcagcaaaga
agacaaagctgaagataaaggcagcaaagaagacaaagctgaagataaag
gcagcatagaagataaagctaaagacaaagacaacaaagaaggcaaagct
gcagacaaaggtatggacaaagcgaaagatgcaatgcatgtcgttcaacc
tggtgaaacagtagaaaaaattgctaaagctaataacacaactgtagaac
aaatcgctaaagataatcatttagaagataaaaacatgattttaccaggt
caaaaacttgttgttgacaaccaaaaagcaatgaaagacagccaagaagc
taaagcaaaccacgaaatgaaagctttacctgaaacaggtgaagaaaacg
atatggcattattcggtacatcacttacaggtggtcttagcttagcatta
ggtttatacatcttaggacgtggcagaaaaacaaactaa
The protein sequence translated from SEQ ID NO 3 is designated SEQ
ID NO: 4 and is shown below:
TABLE-US-00003 SEQ ID NO: 4
MENKNFFSIRKLSIGVGSCLIASSLLVNTPSFAEEGDNNAEAQQNAFSEVVKLPNLSEEQRNGFIQSLKDD
PSTSQDVLNEAKKLNDSQEGSQPAPDYSDEQQNAFYEILHLPNLTEEQRNGYIQSLKDDPSVSANILVEAK
NMNVNQTPTQPAPSFDEAQQNAFYEIVNLPNLTEEQRNGFIQSLKDDPSVSKDILVEAKKLNDSQAKPDYS
EAQQNAFYEILHLPNLTEEQRNGFIQSLKDDPSVSSDILAEAKKLNDSQAPKEDNNVKDNNSGENKAEDKG
NKENKAEDKGSKEDKAEDKGSKEDKAEDKGSKEDKAEDKGSKEDKAEDKGSIEDKAKDKDNKEGKAADKGM
DKAKDAMHVVQPGETVEKIAKANNTTVEQIAKDNHLEDKNMILPGQKLVVDNQKAMKDSQEAKANHEMKAL
PETGEENDMALFGTSLTGGLSLALGLYILGRGRKTN SEQ ID NO: 5
gtgtacaaaaatgaagaagaaaagcattcaataagaaagttatctataggagccgcatct
gtcattgttgggggactcatgtatggtgttttgggaaatgatgaagctcaagcgaatgaa
gatgtcactgaaacaactgggagaaattcagtgacaacgcaagcttctgagcaacatttg
caagtggaagcagtacctcaagaaggcaataatgtaaatgtatcctctgtaaaagtacct
acgaatacggcaacgcaagcacaagaagatgttgcaagtgtatccgatgttaaagcacat
gctgatgatgcattacaagtacaagaaagtagtcatactgatggtgtttcttcagaattc
aagcaggagacagcttatgcgaatcctcaaacagctgagacagttaaacctaatagtgaa
gcagtgcatcagtctgaatacgaggataagcaaaaacccgtatcatctagccgcaaagaa
gatgagactatgcttcagcagcaacaagttgaagccaaaaatgttgtgagtgcggaggaa
gtgtctaaagaagaaaatactcaagtgatgcaatcccctcaagacgttgaacaacatgta
ggtggtaaagatatctctaatgaggttgtagtggataggagtgatatcaaaggatttaac
agcgaaactactattcgacctcatcagggacaaggtggtaggttgaattatcaattaaag
tttcctagcaatgtaaagccaggcgatcagtttactataaaattatctgacaatatcaat
acacatggtgtttctgttgaaagaaccgcaccgagaatcatggctaaaaatactgaaggt
gcgacggatgtaattgctgaaggtctagtgttggaagatggtaaaaccatcgtatataca
tttaaagactatgtaaatggcaagcaaaatttgactgctgagttatcagtgagctatttc
gtaagtccggaaaaagtcttgactactgggacacaaacattcacgacgatgatcggtaat
cattcaacgcaatccaatattgacgtttattatgataatagtcattatgtagatggacgt
atttcgcaagtgaacaaaaaagaagctaaatttcaacaaatagcatacattaaccctaat
ggctatttaaatggcagggggacaattgcagttaatggtgaagtggtcagtggtacgact
aaagacttaatgcaacctacagtgcgtgtatatcaatataaaggacaaggtgttcctcct
gaaagtattactatagaccctaatatgtgggaagaaatcagcataaacgatactatggta
agaaaatatgatggtggctatagcttgaatctggataccagcaagaatcaaaaatatgcc
atctattatgaaggggcatatgatgcgcaagctgacacactgttgtatagaacatatata
cagtcattaaacagttactatccgttcagttaccaaaaaatgaacggtgtgaagttttac
gaaaacagtgcgagtggaagcggtgagttgaaaccgaaaccacctgaacaaccaaaacca
gaacctgaaattcaagctgatgtagtagatattattgaagatagccatgtgattgatata
ggatggaatacagcagttggagaagaaagtggagcaaaccaaggccctcaagaagaaatc
acggaaaatcacgacatcgaagtcattgaggaaaacaacttggtggaaatgacagaagat
acagcagttggagaagaaagtggagcaaaccaaggccctcaagaagaaatcacggaaaat
cacgacatcgaagtcattgaagaaaacaacttagtggaaatgacagaagatacagcgttg
gaagaagaaagtggagcaaatcaaggtcctcaagaagagatcacagaaaaccacgatatc
gaagtcattgaagaaaacaacttggtggaaatgacagaagatacagcgttggaagaagaa
agtggagcaaatcaaggtcctcaagaagagatcacagaaaaccacgacatcgaagtcatt
gaagaaaataacttagtagaaatgacagaagatacagcagttggagaagaaagtggagca
aatcaaggtcctcaagaagagatcacagaaaaccacgatatcgaagtcattgaggaaaac
aacttagtggaaatgacagaagatacagcagttggagaagaaagtggagcaaaccaaggt
cctcaagaagaaatcacggaaaatcacgacatcgaagtcattgaagaaaacaacttggtg
gaaatgacagaagatacagcgttggaagaagaaagtggagcaaatcaaggtcctcaagaa
gagatcacagaaaaccacaacatcgaagtcattgaagaaaacaacttggtggaaatgaca
gaagatacagcagttggagaagaaagtggagcaaacccaggacctcaagaagaagtaaca
gagaatcaacctcagcaagaagaaatcatggaaaaccaagaagtcgaaaagaaaggcgat
agtaacttggtagaaagtacaaaaactccaaaggccgaagaatcagttagcgttcagcca
actttagaagacaaaaacacaaagaaccacgttaacacagtagtagtgaatacgaaggta
tctgaagttaaagaaaaggatccccaccatacaaaagcactaccagatacggggacaacc
tctcgaagtcattccatgatgattcctctccttcttgttgctgggtcagtagtgttgtta
cgtcgaaagaaaaagcatagtaaggtgaattaa The protein sequence translated
from SEQ ID NO 5 is designated SEQ ID NO: 6 and is shown below: SEQ
ID NO: 6
VYKNEEEKHSIRKLSIGAASVIVGGLMYGVLGNDEAQANEDVTETTGRNSVTTQASEQHLQVEAVPQEGNN
VNVSSVKVPTNTATQAQEDVASVSDVKAHADDALQVQESSHTDGVSSEFKQETAYANPQTAETVKPNSEAV
HQSEYEDKQKPVSSSRKEDETMLQQQQVEAKNVVSAEEVSKEENTQVMQSPQDVEQHVGGKDISNEVVVDR
SDIKGFNSETTIRPHQGQGGRLNYQLKFPSNVKPGDQFTIKLSDNINTHGVSVERTAPRIMAKNTEGATDV
IAEGLVLEDGKTIVYTFKDYVNGKQNLTAELSVSYFVSPEKVLTTGTQTFTTMIGNHSTQSNIDVYYDNSH
YVDGRISQVNKKEAKFQQIAYINPNGYLNGRGTIAVNGEVVSGTTKDLMQPTVRVYQYKGQGVPPESITID
PNMWEEISINDTMVRKYDGGYSLNLDTSKNQKYAIYYEGAYDAQADTLLYRTYIQSLNSYYPFSYQKMNGV
KFYENSASGSGELKPKPPEQPKPEPEIQADVVDIIEDSHVIDIGWNTAVGEESGANQGPQEEITENHDIEV
IEENNLVEMTEDTAVGEESGANQGPQEEITENHDIEVIEENNLVEMTEDTALEEESGANQGPQEEITENHD
IEVIEENNLVEMTEDTALEEESGANQGPQEEITENHDIEVIEENNLVEMTEDTAVGEESGANQGPQEEITE
NHDIEVIEENNLVEMTEDTAVGEESGANQGPQEEITENHDIEVIEENNLVEMTEDTALEEESGANQGPQEE
ITENHNIEVIEENNLVEMTEDTAVGEESGANPGPQEEVTENQPQQEEIMENQEVEKKGDSNLVESTKTPKA
EESVSVQPTLEDKNTKNHVNTVVVNTKVSEVKEKDPHHTKALPDTGTTSRSHSMMIPLLLVAGSVVLLRRK
KKHSKVN SEQ ID NO: 7
atgaataaatcaagaactaaacattttaattttttatcaaaacgtcagaatcggtatgct
attcgccacttttcagctggtactgtgtcagtgcttgtaggagcagctttcttgctaggt
gtccatacgagtgatgcatctgctgcagaacaagatcaaacatctgaagcaaagcaaaac
ctctttgatgcttccgctatttttggcgctttaacagagacgaacgaaaaggtagcacaa
gtgacgccaacagaaaaaaatctttcatcagttgaagaaatgagagataaaggcgcaact
ggaaatggaccatcaataacatcactacaaactgtagaacaaaataatgcagtacaacct
acagcaacacctattaatgacacagaaaattcaaccgaagcccctatgaaagaacaatcg
aatgatgcacaaacgactgacgaaagtaacaatgccactcagaaaaataatactgaaccc
caagcaaacaatgaaatatcagcgcgtaatgcaaaaacaacagcatatttaacaagtgaa
acctttacaacagcaacgtctacaactgatatgcctacacagaaacaagaatatccatct
ttagaaaatccaacaaatcaatcgcaaacgaacagagcacaaccaccaacaatggaagca
cccaaactggcagaaggattagacaatctattaaaaaaatcaactttcgaaagtatgtac
gtgacaaaaagaaatcaatttgacaaagagacggcttctaaaacaaaagcatggccgagt
gatgttgttccagaaaatcaagtagagatacttgctgatgcaattcaaaatggctatatc
aaatctgtaaatgatgtgaccaataaagcacatacgttatctggacgtgcatggatgtta
gaccacggaacaccaacgacaatagctaatggtttaacacctgttccagagggcactaaa
gtttatttgcggtggatagatcaagatggtgccacttcgccaatgtatacagcaaaaacg
acaagtagattaagcgctgcggatggtaatcaagtgggtccaggtgcttatgctttcgat
ttacgcacaggttggatagatgctaaaggaaaacaccacgtatatagagcagtaaagggt
caatattataaaatatggatcaatgattttagaactaaagacggtaatatcgctacaatg
ttacgtgttgcaggaggatatgttccgggaacgtacgtggattctgtgacatacaacaat
atgggccaatttccattaattggtacaaatatgcaacgtacaggtatctttatgacaacg
ataccttcagaaaaatatttaatatcaaaacattacgtgaaagatacaaaaggtgctgca
gcaaatccagccgtcacgataattgaaaataactttgtgagcggcaaagtttggatagaa
acaggtgctggagattatgtgaactcagcgacaggtccaaaccacaatgcgaaagatgtc
gttgcctctggatacaaagtggtcatgtcatcattaacagatcaaggtgctaaagcctac
gatgcgcaagtcaatcgcttgccgaagaaagatcgagcagaagcagcacgtcaattatta
ataaaacatccagaatatatcgcagcaactgtagaagggataacgaatgagtgggggaga
tatacattgcgtttccctaaaggcacattcaacaaagaccatctttacggttacgtattg
gattttgatggtgaaattgtaaaaacttattcaggttttacttcaccagagttccggaga
ccgaattataatttgaccgttacaccgcaaacagctccctattatagacccgttcgacgt
gcatgggtcaatgttaattttgcggttattgaagcaccacaatctcaaatcgaaataaaa
gaatttgatgcaacctctaaccctgcgcatcgtgggcaaacagcaactattgatatcata
ggtatgcctaaaacttcattacttacacgtgtacaatggaaagattcatcgggcagtatt
gttgaggatagtggtcctgtttttacggaagaagaggctgaacatatagcggaatttgta
ataccgtctagcgcaaaatcaggcgaagtgtatactgtacaactcgtggtaggtaatcat
atcgtagcttcggactctcttattgtacatgtcaatgaagaagcggcgacatatcatccg
atatacccatcgacaacagtagaatcaggtcaaagagtaacgattccagcacctaagaat
atggatggcaaacctttactagatggcacaacttttgaaaaaggtcatcacgtaccaact
tgggctttagtgaatggtgatggctcgattacagtaaaacctggagaaaaagtagcagag
ggtgagtatgatattccagtgattgtgacatatccagatggttctaaaaacacaatcttt
gcacctgtgaccgttgaagaaaaacaaccaatggcatcgcaatatgagccaataacaact
ggagtatcgaaaccatttggaaacccagtaatgccaactgatgtaacagattcaattcaa
gtaccgaactatccattggaagggcaacaaccgacagtaacagtggatgatgaaagtcaa
ttaccagatggaacaacagaaggttacaaggatatagatgtaacagtgacatacccagac
ggaacaaaggatcgtgtcaaagttccagtcgtaacggaacaacaattagatagtgataaa
tatgatccggtcgcaacaggtatcttgaaaccgtttggtactccaacaacagaggaagac
gttataaaattagtggagataccgaaatatccaacagacttaacacaaccaaaagtaaca
gtgacggttccaaatactttaccggatgggcaaacgccaggtaaagtagacgttgatgtg
acagtaacgtatccagatggttccacagatcacatttcagttccagtttggacaaacaag
catctggataaagacaaatataacccaataacgactggggtatcgaaaccatttggaatc
ccagtaacgccaactgatgtaacagattcaattcaagtaccgaactatccattggaaggg
caacaactgacagtaacagtggatgatgaaacacaattaccagatggaacaacagaaggt
cacaaggatatagatgtaacagtgacatacccagacggaacaaaggatcatatcaaagtt
ccagtcgtaacggaaaaacaatcagataatgaaaaatatgagccaacaactaacggaatc
acgaaaaagtacggtatccctacgacagaggatgaagtgatagatatagttcgaattcca
tattttccagtagatggcgtgcaacctattgtaacggtaaatgatcctagactattgcca
aatggtcaaaaagaaggtcaaatcaatgttccagtcacagtgacgtatccggatggcaca
aaagatctcatgacagttccggttattacaggtaagcaagcagaaaatgaaaaatacgat
ccaatcacattaggagtaactaaagattatggtgatcctacaactgcaaacgatgtgaca
aagtcaatccaaataccaacatatccagcaggtggcgaacaaccaatcgcaacagcggat
gatgaaagtcaattaccggatggcacagtagaaggtaaagtggatattccagtcacagtg
acgtatccggatggtactcaggatcatatcactgtcccagtatttaccaatcaacaacga
gataatcaaaaagccagtaaagctgtgacgaaaatacatggtatatcggtaacaggcact
gatatgacagatactaagaaaaatcataactatccagcaggtggtgaacaacctaaagtt
actgtgaaagatgacgatcaattatcagagggtaaagtcgattcaacagtgggtgcggat
aatgtgacaactacagatgatttatcaagcgtaactgcggtatctcatggtcatcaaaca
agtgtacaaacaacaaaagagaaccaatcagtgcatgatgaagaggtgacgatcccaaca
gttgcacatgtgtctacaataatgacaggtgtggtaaagggtgagcaagaagcgacggat
atcgtggctagaccacatgttgaaacaactcaactcccatcaatttcagctcaagcaaca
gttaaaaaactaccagaaacgggtgaaaacaatgaacaatcaggtgttttattaggtgga
tttattgcgttcatgggtagcttacttttattcggcagacgtcgcaaaccaaagaaagat
taa
The protein sequence translated from SEQ ID NO 7 is designated SEQ
ID NO: 8 and is shown below:
TABLE-US-00004 SEQ ID NO: 8 MNKSRTKHFN FLSKRQNRYA IRHFSAGTVS
VLVGAAFLLG VHTSDASAAE QDQTSEAKQN LFDASAIFGA LTETNEKVAQ VTPTEKNLSS
VEEMRDKGAT GNGPSITSLQ TVEQNNAVQP TATPINDTEN STEAPMKEQS NDAQTTDESN
NATQKNNTEP QANNEISARN AKTTAYLTSE TFTTATSTTD MPTQKQEYPS LENPTNQSQT
NRAQPPTMEA PKLAEGLDNL LKKSTFESMY VTKRNQFDKE TASKTKAWPS DVVPENQVEI
LADAIQNGYI KSVNDVTNKA HTLSGRAWML DHGTPTTIAN GLTPVPEGTK VYLRWIDQDG
ATSPMYTAKT TSRLSAADGN QVGPGAYAFD LRTGWIDAKG KHHVYRAVKG QYYKIWINDF
RTKDGNIATM LRVAGGYVPG TYVDSVTYNN MGQFPLIGTN MQRTGIFMTT IPSEKYLISK
HYVKDTKGAA ANPAVTIIEN NFVSGKVWIE TGAGDYVNSA TGPNHNAKDV VASGYKVVMS
SLTDQGAKAY DAQVNRLPKK DRAEAARQLL IKHPEYIAAT VEGITNEWGR YTLRFPKGTF
NKDHLYGYVL DFDGEIVKTY SGFTSPEFRR PNYNLTVTPQ TAPYYRPVRR AWVNVNFAVI
EAPQSQIEIK EFDATSNPAH RGQTATIDII GMPKTSLLTR VQWKDSSGSI VEDSGPVFTE
EEAEHIAEFV IPSSAKSGEV YTVQLVVGNH IVASDSLIVH VNEEAATYHP IYPSTTVESG
QRVTIPAPKN MDGKPLLDGT TFEKGHHVPT WALVNGDGSI TVKPGEKVAE GEYDIPVIVT
YPDGSKNTIF APVTVEEKQP MASQYEPITT GVSKPFGNPV MPTDVTDSIQ VPNYPLEGQQ
PTVTVDDESQ LPDGTTEGYK DIDVTVTYPD GTKDRVKVPV VTEQQLDSDK YDPVATGILK
PFGTPTTEED VIKLVEIPKY PTDLTQPKVT VTVPNTLPDG QTPGKVDVDV TVTYPDGSTD
HISVPVWTNK HLDKDKYNPI TTGVSKPFGI PVTPTDVTDS IQVPNYPLEG QQLTVTVDDE
TQLPDGTTEG HKDIDVTVTY PDGTKDHIKV PVVTEKQSDN EKYEPTTNGI TKKYGIPTTE
DEVIDIVRIP YFPVDGVQPI VTVNDPRLLP NGQKEGQINV PVTVTYPDGT KDLMTVPVIT
GKQAENEKYD PITLGVTKDY GDPTTANDVT KSIQIPTYPA GGEQPIATAD DESQLPDGTV
EGKVDIPVTV TYPDGTQDHI TVPVFTNQQR DNQKASKAVT KIHGISVTGT DMTDTKKNHN
YPAGGEQPKV TVKDDDQLSE GKVDSTVGAD NVTTTDDLSS VTAVSHGHQT SVQTTKENQS
VHDEEVTIPT VAHVSTIMTG VVKGEQEATD IVARPHVETT QLPSISAQAT VKKLPETGEN
NEQSGVLLGG FIAFMGSLLL FGRRRKPKKD SEQ ID NO: 9
atgtttaatcaacaaaaacaacactatggtatccggaaatatgcaatcgggacttcatca
gtattattaggcatgacattatttatcacacatgacgcaactgcatctgcagctgaaaac
aatacaactgcaaagacagagacaaatcaagcagcaacaatttcttctcgcacttcgcca
accgacgtcgctcaacctaatgcagacacgaatgctacaacggcgactaaagagacaaca
ccacaatcagattcaacagcattaccgcaagcagcagcgcaacctcaaacgggccaaaca
gcatcgaaagacacagtagatacaaataaaacgcaaacagcagattccacaaccgctcct
cctgtgacagacgcgccaaaagctaatgacgacacaacacagccagaagctgcgactgta
gccaaaaaagaagatgctcagacaccatcgactgcagaccctacaccacaagcgcaacaa
ccgcctcagtcaaaagcacctcaagaaacgcaacaacaatcaacagttgaagatacaacg
ccacaacaaaacgcatcaactgaagcacaccctaaaaatgtagataccgcttcaacaaaa
caacaacaaacaacgccatcaaccgcaccgacaccttacacacaacaagcagacgaagca
atgacagatgtcacaacaaccagtgtcgacagcaacgtacagccgttagcccctgcagaa
gatcaacctaaaaatacgaacacagctgacaaagcaaccgttgcgacaccaccacgtgac
aatgctaagactgctgatccgaacaaaaagatgacacgtgcagcaacgacacaacaagat
gatgccgtcgatacattgaagtcaaaagaaatgacagcaacgatcgataaaagttttcca
gccgttaaatattacacgttgaaaaatggtaaaaaagtcgatgcacaactgacggatgca
cgtcaaatcatcgtcaatggtgaagtcattacaccaacagtcaaatacaacaaaattgat
gatcatacggctgaatatgacttaacagcacaaaatgattcacgttcgattgatgccaat
tttaaatttcgtttatcagttgaaggtaagaccgttgatttacaaatgacagattacacg
aacaacaacacagatccacaaaacgtcattcgcaactttagctttgtaagtcaatcgctc
gtatctgtaaacaatcaacagaaaaatgccaaactgcaaacatcgaaactgtctacaaat
acaatgaaaagcggcgataaatcatatcatatcgatgaaaatttcaaaaacgacttcaac
gactttatgatgtacggtttcgtgtcaaatgatgattacagtgcaggattgtggagtaac
gcacaaattggcgtcggcattggtgaacaagacttcttacgtgtctacgcacagtctata
caaacagatatcggggtcgctgtcggtttaggctcaatgccatggtttatccaaaaagac
gctgcacatccagatgcgaaaaatcaaggactactcccacatgtcaaagttgcaattgcg
gaagatgaaaatcaagatggtgaaattaactggcaagacggtgcaattgcttatcgtagc
attatgaacaatccatatggtgccgaagaagtacctgaccttgttgggtaccgtatcgcg
atgaactttggttctcaagcgcaaaacccatttttaaagacgttagatggtgtgaaaaaa
ttctatctcaatacagatggtttagggcaatccattttattaaaaggttataacagtgaa
ggccacgactctggtcatttagattacgcgaatattggtcaacgtataggtggcgtgaaa
gactttaaaacgttacttcaaaaaggggcagattatggcgcacgtttcggtcttcatgtg
aatgcatctgaaacatatccagagtctcaagcattcaatcctgccctcttacgtaaagat
gcgaatggaaactatatgtatggctggaactggctcgatcaaggctttaacatcgatgca
gattacgatttaatacacgggcgtaaagaacgcttcgaagcactcaaacaaattgtcggt
gatgacctcgactttatttatgtcgatgtatgggggaatggacaatccggcgacaataca
gcttggccatcacatcaattagccaaagaaatcaacgacttaggatggcgcgtcggtgtc
gaatggggtcacggtatggaatatgactccacgttccaacattgggcagccgacttaacg
tatggatcgtaccaaaataaagggattaactcagaggtagcacgcttcttacgcaaccat
caaaaagattcatgggtcggtaactatccaaaatactcaggtgcagctgacttcccattg
ctcggcggttatgacatgaaagattttgaaggttggcaaggtcgtaacgattactctgct
tacattaaaaatattttcaatgttgatgtaccaacaaagtttttacaacattataaagtg
atgcgtattgtcgatggtgagcctgttaaaatgactgccaatggtcaaacgattgactgg
acaccagaaatgcaagtcgatttacaaaatgaagccggtgatcaagtcactgttaaacgt
aaatctaacgactatgaaaacgacactgacaactaccgctcacgtacaatcgaattgaat
ggtcgcacagtactcgatggcgattcataccttttaccatggaattgggatgcgaacggc
caaccattaactggcgataacgaaaaattatatcactggaataaaaaaggcggttcaacg
acttggacactgcctgaatcatgggatacagaccaagtcgtgctatacgaattatctgaa
acgggtcgtaagtcaccacgtacagtggcagtgaaagaccatcaagtgacactcgataat
attaaagcagacacaccgtatgtcgtttataaagtcgcacaaccagacaacacagatgtg
aactggagcgaagacatgcacgtgaaagatgccggcttcaactcacaacaactgacacct
tggacaatcgaaggcaatcgagataaagtgagcatcgaaaagtcgacaacatcaaatgaa
atgctaaaaatcgatagtccaacaaaaacaacgcaattaacgcaacaattgacaggttta
gtgccaggacaacgttacgctgtctatgttggcatcgataaccgcagtgatgcagcggcg
catattgcagtgacacataacggtaaaacgctcgcaagtaacgaaacaggtcaatcgatc
gcgaaaaactatgtgaaagcagatgcacatagtaacaatgctgcgacgtttaaaaatggc
ggtagttacttccaaaacatgtacgtgtacttcgttgcgccagaagatggtaaagcagac
ttgacgattcaacgcgacccaggtgaaggggccacttatttcgatgatattcgtgtgtta
gaaaataacgcgaatctccttcaaaacggcacattcaaccaagacttcgaaaatgtacca
caagggttattcccgttcgtcgtgtcagaagttgaaggcgttgaagataatcgcgttcac
ttatctgaaaagcacgcaccgtatacacaacgcggatggaataataaacgtgtcgatgat
gtcattgatggcaaatggtcacttaaagtaaacggtcaaacaggtaaagataaaatggtc
atccaaacgattccgcaaaacttctacttcgaaccaggaaaaacgtatgaagtgtcattt
gattatgaagcaggttctgatgatacgtatgcatttgcgacaggtagtggggacatttct
aaaaatcgtaactttgaaaagacaccattgaaaaatacagtcgatggtggcaaagcgaaa
cgggtgacatttaaagtgacgggtgatgaaaatggtcaaacttggatcggtatttactca
acgaaaacacccaatgatccacgaggcgtgaaaaatggcaatcaaatcaacttcgaaggg
acgaaagatttcattctagacaacctttctatccgtgaaattgacgcaccgaagcctgat
gccacacaagaaagcggtgatagcgcaccaatgaatgaaacagatgagcgtaacgtcaat
tcaaacggtacattagccgatcatagtgagacaactgatgtcaatgtcagtgcaacggca
gatgatacagcagtcaaaggcgaaatgacgacaaacagaacagatgcaccaactgttaca
ctgcctgaagcaacgatagtagatgaaggcacgtcaaatcctgtcactacaacaccaacg
aatacaacacaagctatgacaaataaggctgatgagatgccacaaacgatgaacaatgtt
cctttaactagcatcgctaccgatatgatgcagtctcatgcggtggattccatggcagca
acactagcagctacaaatcaagtggcggcacctgtgcgtcaaacagcaggacctatgcaa
catggtatggacagtgcttcaacgcaacacgcacccatacaagttgacaatgtcacagca
ccaccattaccagatgaacagtttgccgaattacctaaaactggggatacgactccaaat
acacgtggacctttaatggcgatgatagttggcgcagtcttaacagcattcggattcaga
cgccaacgtaaagaaaaatag
The protein sequence translated from SEQ ID NO 9 is designated SEQ
ID NO: 10 and is shown below:
TABLE-US-00005 SEQ ID NO: 10
MFNQQKQHYGIRKYAIGTSSVLLGMTLFITHDATASAAENNTTAKTETNQAATISSRTSPTDVAQPNADTN
ATTATKETTPQSDSTALPQAAAQPQTGQTASKDTVDTNKTQTADSTTAPPVTDAPKANDDTTQPEAATVAK
KEDAQTPSTADPTPQAQQPPQSKAPQETQQQSTVEDTTPQQNASTEAHPKNVDTASTKQQQTTPSTAPTPY
TQQADEAMTDVTTTSVDSNVQPLAPAEDQPKNTNTADKATVATPPRDNAKTADPNKKMTRAATTQQDDAVD
TLKSKEMTATIDKSFPAVKYYTLKNGKKVDAQLTDARQIIVNGEVITPTVKYNKIDDHTAEYDLTAQNDSR
SIDANFKFRLSVEGKTVDLQMTDYTNNNTDPQNVIRNFSFVSQSLVSVNNQQKNAKLQTSKLSTNTMKSGD
KSYHIDENFKNDFNDFMMYGFVSNDDYSAGLWSNAQIGVGIGEQDFLRVYAQSIQTDIGVAVGLGSMPWFI
QKDAAHPDAKNQGLLPHVKVAIAEDENQDGEINWQDGAIAYRSIMNNPYGAEEVPDLVGYRIAMNFGSQAQ
NPFLKTLDGVKKFYLNTDGLGQSILLKGYNSEGHDSGHLDYANIGQRIGGVKDFKTLLQKGADYGARFGLH
VNASETYPESQAFNPALLRKDANGNYMYGWNWLDQGFNIDADYDLIHGRKERFEALKQIVGDDLDFIYVDV
WGNGQSGDNTAWPSHQLAKEINDLGWRVGVEWGHGMEYDSTFQHWAADLTYGSYQNKGINSEVARFLRNHQ
KDSWVGNYPKYSGAADFPLLGGYDMKDFEGWQGRNDYSAYIKNIFNVDVPTKFLQHYKVMRIVDGEPVKMT
ANGQTIDWTPEMQVDLQNEAGDQVTVKRKSNDYENDTDNYRSRTIELNGRTVLDGDSYLLPWNWDANGQPL
TGDNEKLYHWNKKGGSTTWTLPESWDTDQVVLYELSETGRKSPRTVAVKDHQVTLDNIKADTPYVVYKVAQ
PDNTDVNWSEDMHVKDAGFNSQQLTPWTIEGNRDKVSIEKSTTSNEMLKIDSPTKTTQLTQQLTGLVPGQR
YAVYVGIDNRSDAAAHIAVTHNGKTLASNETGQSIAKNYVKADAHSNNAATFKNGGSYFQNMYVYFVAPED
GKADLTIQRDPGEGATYFDDIRVLENNANLLQNGTFNQDFENVPQGLFPFVVSEVEGVEDNRVHLSEKHAP
YTQRGWNNKRVDDVIDGKWSLKVNGQTGKDKMVIQTIPQNFYFEPGKTYEVSFDYEAGSDDTYAFATGSGD
ISKNRNFEKTPLKNTVDGGKAKRVTFKVTGDENGQTWIGIYSTKTPNDPRGVKNGNQINFEGTKDFILDNL
SIREIDAPKPDATQESGDSAPMNETDERNVNSNGTLADHSETTDVNVSATADDTAVKGEMTTNRTDAPTVT
LPEATIVDEGTSNPVTTTPTNTTQAMTNKADEMPQTMNNVPLTSIATDMMQSHAVDSMAATLAATNQVAAP
VRQTAGPMQHGMDSASTQHAPIQVDNVTAPPLPDEQFAELPKTGDTTPNTRGPLMAMIVGAVLTAFGFRRQ
RKEK SEQ ID NO: 11
atgacaagaaaatttagggaatttaagaaaagtttaagtgaagaaaaagcaagagtgaaa
ctttacaagtcaggtaaaaactgggttaaagctggaattaaagaatttcagttattaaaa
gcattaggcttatcttttttaagccatgacattgtaaaggatgaaaatggagaagtaacg
acacaatttggggaacagttgaagaaaaatgcattaagaacaactgcttttgcgggtgga
atgttcacagttaatatgttgcatgaccaacaagcatttgcggcgtcggatgcacctata
acttctgaactggcaaccaaaagtcaaactattggcgatcaaacatcaattgttattgaa
aagtctacatcgtcagatcaatcaacgaacccaataacagaaagtgaaagtaaacacgat
tctgaaagtatctcattatctgagcatcaaacatcagagtcaacaagtctttcaacgtca
acttccaaatcaatatcaacttcagtagaggaatcagaatcaacatcaaaagattctcat
actaaaactcaagatagtcaatcagatagtcatcagtcaacaagtcaagaggtaaatggc
tcttccaaccacgagcaatcaacaccacacactgcacaaagtcttacgagcctatctatt
gagagccaaacgtcgacttcaaatacatcattgaaggaaactaaagaaggggaattgtca
aaaaacctttcgaagttatctcaaaatcaaaacatcaaacttcatgaagaacatacgatg
cgttcagcagatttgagctcaggttatacaggatttagagcggcttactatgtaccaaga
tcaagaacaacaccaacgacaaaagtctacacagggcaaggaagcttcagaggtagaggt
agaattaaatataatattttctacaaagttgtcgttacaagtaatggcaaagaaatgaag
atccgctatacattgagtcaagatgatccaaacacgtctaatgttgaaaaacctaggtgg
gcaggacagaaacgatttggtattcataatacttgggatgaaggtcctggtcgcgggcaa
ttaaagttagggtcggcattcggcaaaccaacagttatacaaggagaaactagaccgaat
tatggtagctgggttggcacacctataacgaaatatgtttcaggcgatcgtacaaatggt
ttttactggcaagctgctgtacttgcaccgagacatggagagaagggagaaggaatcaca
gcagaaattacagttcctattgttaacccttctggaagatttaattgggaattccatcct
gtcggtcaacaggacggagttggtggcaaaactgactactttgaaaatgtatggattcga
gactatgacccatattacaaatatattcaaactaaggaaggcagagcctcagtttcgcac
tctatttctcaggtgaaagcaagtgaatcgagatcgacatcgctcatacaatcggagtct
attagaagatcacagtccatatctgagagtgaatctattgtagccgcaagtcattcggca
agtgtagcaaaatcgcaatccatctcgagaagtcaatctgtggcgaaatcacaatcgatc
tcaagaagtcagtcgatcgcacacagccgatcagcaagtgtggcaaaatcgcaatccatc
tcaagaagtcagtcgatcgcacacagccgatcagcaagtgtggcaaaatcacaatcgatt
tcaagaagtcagtcgatcgcacacagccgatcagcaagtgtggcgaaatctcaatcgatt
tcaagaagtcagtcaattgcgcagagccaatcagcaagtgtggcaaaatcacagtcgatt
tcaagaagtcagtcaattgcgcagagccaatcagcaagtgtggcgaaatcgcaatcgatt
tcaagaagtcagtcgattgcacatagccgatcagcaagtgtagcggaatcacagtcgatt
tcaagaagtcagtcgattgcgaatagccaatctgtagcagcgagtgaatcagagagtcta
tcaatatcattgtctaaaaagcagtcaatatcgatgagtaattctgaaagtgcagcaaaa
tcacactcgctttcggtgaaaaggtctaactggattaaaaagtcaaaagcggcttcagta
agaaagtcacattcactttcggtaagaaaatctaattcggcgaaaaggtcacatgctatt
tcggtaagaaagtcaaagtcattatcagttaaaaagtcaatttcgcagagccaatcagca
agtgtggcgaaatcgcaatcgatttcaagaagtcaatcagtagcagcgagtgagtcggca
tcgctaagtaagtcgaagagcacatcgctcagtaactcagtgagtgcagagaaatcgacg
tcattaagtcgttcagcaagtgtagcaaaatcgcaatcgatttcaagaagccaatcagta
gtagcgagcgaatcggcatcgttaagtaagtcgaagagcacatcgctcagtaactcagtg
agtgcagagaaatcgacgtcattaagtcgatcagcaagtgtagcaaaatcgcaatcgatt
tcaagaagccaatcggtggcagcgagcgaatcggcatcgttaagtaagtcgaagagcaca
tcgctcagtaactcagtgagtgcagagaaatcgacgtcattaagtcgatcagcaagtgta
gcaaaatcgcaatcgatttcaagaagccaatcggtggcagcgagcgaatcggcatcgtta
agtaagtcgaagagcacatcgctcagtaactcagtgagtgcagagaaatcgacgtcatta
agtcgatcagcaagtgtggcaaaatcgcaatcgatttcaagaagccaatcagtagtagcg
agcgaatcggcatcgttaagtaagtcgaagagcacatcgctcagtaactcagtgagtgca
gagaaatcgacgtcattaagtcgatcagcaagtgtagcaaaatcgcaatcgatttcaaga
agccaatcggtggcagcgagcgaatcggcatcgttaagtaagtcgaagagcacatcgctc
agtaactcagtgagtgcagagaaatcgacgtcattaagtcgatcagcaagtgtggcaaaa
tcgcaatcgatttcaagaagccagtcagtagcagcaagtgagtcggcatcattaagtaag
tcgaagagcacatctttaagcaactcagtgagtgtagagaaatcgacgtcattaagtcga
tcagcaagtgtggcgaaatcgcaatcgatttcaagaagtcaatcagtagcagcgagtgag
tcggcatcgctaagtaagtcgaagagcacatcgctcagtaactcagtgagtgcagagaaa
tcgacgtcattaagtcgttcagcaagtgtagcaaaatcgcaatcgatttcaagaagccag
tcagtagcagcaagtgagtcggcatcattgagtaaatcaacaagtacgtcaacaagtgac
tcagatagcgcgtcaacatcaacatctgtatcagatagcgattcagcttcattgagtaag
tcgactagtacatcaacaagcgattcagacagcgcgtcagcatcattgagcaagtcaaca
agtacatcaacgagcgactcagatagcgcatcgacatcaacatcagtatcagatagcgac
tccgcatcgttgagtaaatcgacaagcacgtcaacaagtgattcagacagcacgtctact
tcattgagtaagtcgacaagtacatcgacaagtgattcagatagtgcgtcaaaatcaacg
tcagtatcagacagtacgtccgcatcattgagtaaatcgacaagcacgtcaacaagtgat
tcagatagtgcatcaaaatcaacgtcggtatcagatagcacgtcagcatcattaagaaag
tcggcaagtacgtcaacgagtgactcagacagcacgtctacttcattgagtaagtcgaca
agtacatcgacaagtgattcagatagtgcatcaaaatcaacatcagtatcagatagcgat
tcagcttcattgagtaagtcgactagtacatcaacaagcgattcagatagtgcgtcaaaa
tcaacgtcggtatcagatagcgactccgcatcgttgagtaagtcgacaagtacgtcaaca
agcgattcagacagtgcatcaaaatcaacgtcggtatcagacagtacgtcaacatcatta
agtaagtcgacaagtacatcaacaagcgattcagatagtgcgtcaacatcgacatcagta
tcggacagtacgtctgcatcattgagtaagtcgacaagcacatcgacaagtgattcagat
agcgcatcaacatcagtgtcagatagcgattcagcatcactaagcaagtcaacaagtaca
tcgacaagcgattcagacagcgtatcaacatcaacatcagtatcagatagtgattccgcg
tcattaagtaagtcgacaagtacgtcaacaagcgattcagatagtgcgtcaaaatcaaca
tcagtatcagatagcacgtcaacatcattgagtaaatcaacaagtacatcgacaagtgac
tcagatagtgcgtcaacatcggtatcagacagtacgtccgcatcattgagtaaatcgaca
agcacgtcaacaagtgattcagatagtgcatcaaaatcaacatcagtatcagatagcgat
tcagcatcattaagcaagtcgacaagtacatcgacaagtgattcagatagtgcgtcaaca
tcaacgtcagtgtcagatagcgattcagcttcattaagcaaatcaacaagtacgtcaaca
agtgactcagatagcgcatcaacatcattaagcaagtcaacaagtacatcgacaagcgat
tcagacagtacgtctacatcattaagtaagtcaacaagtacatcaacaagtgattcggat
agtgcgtcaaaatcaacatcagtatcagatagcgactcagcttcattaagcaagtcgaca
agtacgtcaacaagtgactcagacagtgcgtcaaaatcaacatctgtgtcagatagcgac
tccgcatcgttgagtaagtcgacaagtacgtcaacgagcgattcggatagtgcgtcaaaa
tcaacatcagtatcagatagtgaatccgcgtcattaagcaagtcgacaagcacatcgaca
agtgactcagatagtgcgtcaacatcgacatcggtatcagacagcacatcagtttcatta
agcaagtcgacaagcacgtcaacaagcgattcagacagtacgtctacttcattaagcaag
tcgacaagcacgtcaacaagtgactcagatagtgactcagcttcgttgagtaaatcgaca
agcacgtcaacgagcgattcagatagcgtgtcaacatcaacatctgtgtcagatagcgat
tcagcttcattaagcaaatcgacaagtacatcaacaagcgattcagatagtgcgtcaaca
tcaacgtcggtatcagatagcggctccgcatcgttgagtaagtcgacaagtacgtcaacg
agcgattcagacagtgcatcaaaatcaacgtcggtatcagatagtgattcagcatcacta
agcaaatcgacaagcacgtcaacaagtgactcagacagtgcgtcaacatcgacatcggta
tcagatagcacatccgcgtcgttaagcaagtcgacaagtacgtcaacaagtgattcagac
agcgcatcgacatcaacatcagtatcagatagcgactccgcatcgttgagtaaatcgaca
agcacgtcaacaagtgattcggacagtgcgtcaaaatcaacatcagtgtcagatagcgat
tcagcttcattgagtaagtcgacaagcacgtcaacaagcgaatcagacagcgcgtcaaaa
tcaacgtcagtgtcagatagcgattccgcatcattaagtaaatcgacaagcacgtcaaca
agtgactcagatagtgcatcgacatcaacgtcagtatcagatagtgattccgcgtcatta
agcaagtcgacaagtacgtcaacaagtgactcagacagtgcgtcaaaatcaacatcagta
tcagatagcgattccgcatcattgagtaagtcgacaagcacgtcaacaagcgaatcagac
agtgcgtcaacatcgacattagtatcggatagtacgtcggtttcattgagccaatcaaca
agtgtggataaagatagtacagcgaagggatcgacagaattagtaaatgttgcatcactt
tcaatcagtgcgagtcaatcaagtagtttatctgcttcaacatccacatcgattgaaaag
tctgagtctacatcaacaagtggctcaaattcaactaatgcgtcgttaagtagctcatct
tcacttagtacatcagcaagtacttctgtaagcgaagtgacatctgtcacacattctgaa
aatgatttaagtgcatctaacgatagagatacatccggatcagtaagtcaatttgcttct
gaaaatacatcattaagtgattctgcatcaattagtggcgaagtttctagtagtacgtcc
gcgtcaacttcgaaatcatcatcactttcagcaagcgcgttacatgataagcatgtatca
gaaagcacttctgcatcattaagtagtggagattcaagtcgtgcttcggcatcagtgtca
acgtcattatcagaatcagatagtgcgttaatagactctgaatcaattagcgtttccgag
cacacatcaacattacaatcaggtagtcattcactatcacaacaacaatcagcagaatta
tcacaatcagagcaaacatcacaatcacaacgcatttcaacaagtgcgtcagtatcggct
atgaaatcagaaagtgctgctaaggtatctgaatcgctatctacgtctcaatcaaaagta
gatagtcaatcacaatcggtatctgaatcagcgagcaactcacgagtgtcaagagattca
aaatcaacaagcgcttcaatgcatcgatcattgtcagagtcagtatctcaaagtatgtca
cttattgatcagtcagaaagtgattcaacatctatatcgatttcgacgtcaatcagtgat
gaagactctatgctgtattctatgagtgattccgcatcgatcagtactaaggcatcaagt
agtatgtctacttcgacaagcgaagagcatgccaacagtcattctcagtctgaatcgaca
gcatcggttgaagtatctcaagaaatgagtgcatcggcttcaacaagcaaatctgagtct
caatcagagtcagtatcagtaagtaacgaagaatcaaatatctcatctatgcaagagtct
tttgtagagagtgcaaaagcatcgcgtagtgcatctatgagcgttgcaaaatctgaagcc
tctgaatcacagctattaagtgagtctaatgcttcggtaagccaatcagcaagcacaagt
agtaaagcatcagcaagtacgtcagaatctatttcaacgtcactcagcgtatctgaagca
actcatggaaaaccgagaaatcattcagaaagtgcatcagcaagtcaattattagaagaa
aatgagtcattaagcgattcagcatcaacaagtgttgaagattcagaaagtgcatcagca
tctctgtcggtgtatcaatcacaatcagcaagtgcattgaaatcaacacatgcatcagaa
aaagcttcagtgaatacaagtgcaaacgcatcgaagcgtgcatcagcatcgacatctatc
tctaactcgaaatctaaagtcattgcgagtgaatcgaagtcaacaagcatatcaacatat
gaatcgttgtcaatatcgactagtaaagaacaatcaacgcgtgtatcagtgagtgagtcg
acatcaacgtctaaagtgaagtcagaaagcgactcggcatcaacgtcgacatctgaatca
atctcaattagcgcaaatcgttcaggttacacatcgtctaaacgttcggtacaaatgagt
gaagcacaatcaacgagcgattcattatcagtaatgcaatctgaaggttcagtaagtgta
tcgcaatctttaagtatatcagataagacatcacagtccttatcggaatcaatatcgcat
tcagaaagtgactctgatagtaactcagtgtctattagtcaagagacatctgaacaacat
tcggtgtcagacagtgactcgatgtcaatttcggaaagcgaatctattgcatatagtcaa
tcagcgagtgaatcagaatcaacaagtatcgcaaaatctgatagtatttcgaactcatta
tctgtttcattaagtgaatcagaaagtgaagcaagcacatcagcttcagtgagtacatct
gaaagtacgtctgtaaagggttctctatcaacaagtatcttgaacagtcaatcagcatct
actcatcaatcaacagaagcttctcaaagtacatcaacttcaaaagttgaggaagcatca
ttgagtgactctgcttctgtatcagattcacaatcactttcaatgagtcatgagaaatca
caaagtgcatcgacttcaaaatctacgagtctgtcaaaaactatttctgagtcagagtct
gtgagtgcatcaacatcaacaagtgaagctgtaagtacagaagcaagcgaatttgtatca
gcagtagactcattgagtcaagtaacttctaacggaagcacaacgaaagaagatgcgagt
acatttgtatccacagtagattcattgaaagacaaagcatcaaataatggtacaccatca
gagtttgcgtcagcagtgaaatcaacacacgcatcagtgagtgtgtcagcatcagaaagt
acgtcagcatcaacatcaacaagtgaagctgtaagtacagaagcaagcgaatttgtatca
gcagtgaattcgttgagtgaagcgacttctaacggaagcacaacgaaagaagatgcgagt
acatttgtatccacagtagattcattgaaagacaaagcatcaaataatggtacaccatca
gagtttgcgtcagcagtgaaatcaacacacgcatcagtgagtgtgtcagcatcagaaagt
acatcagcatcaacatcaacaagtgaagctgtaagtacagaagcaagcgaatttgtatca
gcagtagactcattgagtcaagtaacttctaacggaagcacaacgaaagaagatgcaagc
acatttgtatccacagtagattcattgaaagataaagcatcaaacaatggtacaccatca
gaatttgaatcagttgtgaaatcagtacacggatcaatgagtgcatcagcaagtgcgtca
acatcagcatctacatcagcatctacatctacaagtgaagctgcaagtgcagaagcaagc
gaattagaatcagtaaggaaatcattatccaatggagcatcaaacggtagcacagcaaga
gaaggtgcaagcacatttgtatcaacggtagattcattgaaagataaagcatcaaacaat
ggtacagcatcagaatttgaatcagttgtgaagtcagtacacggatcaacaagtgcatca
gcaagtgcgtcaacgtcagcatcaacatcagcaagtgaatcagcaagtacagaagcaagt
gaatttgtatcagcagtggcatcattaagcagttcagcatggaacggaagcactacagga
gaaggtgcaagcacatttgtatcaacagttgattcatcgaaagattcagcgtcagacaaa
gcttcaccatcagaatcagaatcagttgtgaagtcagtacacggatcaacgagtacatca
gcaagtgtgtcagcgtcggcaagtacatcagcatcgacatcaacaagtgaagctgtaagc
acagaagcaagtgagtttgtatcagcagtgaactcattaagcagtgaagcatcgaacggc
agcacaacaagagaaggtgcaagcacatttgtatcaacagtagattcattgaaagacaaa
gcatcaaacaatggtacagcatcagaatttgaatcagttgtgaagtcagtacacggatca
atgagtacatcagcaagtgtgtcagcatcagaaagtacgtcggcatcgacatcgacaagt
gaagctgtaagtacagaagcaagcgagtcagcatcgataagtgtatcaatgtcagtgagc
gcatcaacaagtgcttcaatgagcgtatcagtgtcaaacagtgtgtcagtgagtgactct
atttcagtaagtgcatcaacaagtgaacctaactcggtaagcacttctatgagtagttct
ctttcaacatcggcatcaacgccatcagaaattacttcaagttcgtcatcaagcgattca
gcgacagttcaaaaagtagtttctaaagatgaacagcacgctacaaataaagttgaaaaa
ttacctgacacaggtcaatcaacgacacaaactggtttattgggtggagtaggtgcttta
cttacaggccttggtttactcaaaaaatcaagaaaacaaaaagatgaagaaacatcatca
catgaataa
The protein sequence translated from SEQ ID NO 11 is designated SEQ
ID NO: 12 and is shown below:
TABLE-US-00006 SEQ ID NO: 12 MTRKFREFKK SLSEEKARVK LYKSGKNWVK
AGIKEFQLLK ALGLSFLSHD IVKDENGEVT TQFGEQLKKN ALRTTAFAGG MFTVNMLHDQ
QAFAASDAPI TSELATKSQT IGDQTSIVIE KSTSSDQSTN PITESESKHD SESISLSEHQ
TSESTSLSTS TSKSISTSVE ESESTSKDSH TKTQDSQSDS HQSTSQEVNG SSNHEQSTPH
TAQSLTSLSI ESQTSTSNTS LKETKEGELS KNLSKLSQNQ NIKLHEEHTM RSADLSSGYT
GFRAAYYVPR SRTTPTTKVY TGQGSFRGRG RIKYNIFYKV VVTSNGKEMK IRYTLSQDDP
NTSNVEKPRW AGQKRFGIHN TWDEGPGRGQ LKLGSAFGKP TVIQGETRPN YGSWVGTPIT
KYVSGDRTNG FYWQAAVLAP RHGEKGEGIT AEITVPIVNP SGRFNWEFHP VGQQDGVGGK
TDYFENVWIR DYDPYYKYIQ TKEGRASVSH SISQVKASES RSTSLIQSES IRRSQSISES
ESIVAASHSA SVAKSQSISR SQSVAKSQSI SRSQSIAHSR SASVAKSQSI SRSQSIAHSR
SASVAKSQSI SRSQSIAHSR SASVAKSQSI SRSQSIAQSQ SASVAKSQSI SRSQSIAQSQ
SASVAKSQSI SRSQSIAHSR SASVAESQSI SRSQSIANSQ SVAASESESL SISLSKKQSI
SMSNSESAAK SHSLSVKRSN WIKKSKAASV RKSHSLSVRK SNSAKRSHAI SVRKSKSLSV
KKSISQSQSA SVAKSQSISR SQSVAASESA SLSKSKSTSL SNSVSAEKST SLSRSASVAK
SQSISRSQSV VASESASLSK SKSTSLSNSV SAEKSTSLSR SASVAKSQSI SRSQSVAASE
SASLSKSKST SLSNSVSAEK STSLSRSASV AKSQSISRSQ SVAASESASL SKSKSTSLSN
SVSAEKSTSL SRSASVAKSQ SISRSQSVVA SESASLSKSK STSLSNSVSA EKSTSLSRSA
SVAKSQSISR SQSVAASESA SLSKSKSTSL SNSVSAEKST SLSRSASVAK SQSISRSQSV
AASESASLSK SKSTSLSNSV SVEKSTSLSR SASVAKSQSI SRSQSVAASE SASLSKSKST
SLSNSVSAEK STSLSRSASV AKSQSISRSQ SVAASESASL SKSTSTSTSD SDSASTSTSV
SDSDSASLSK STSTSTSDSD SASASLSKST STSTSDSDSA STSTSVSDSD SASLSKSTST
STSDSDSTST SLSKSTSTST SDSDSASKST SVSDSTSASL SKSTSTSTSD SDSASKSTSV
SDSTSASLRK SASTSTSDSD STSTSLSKST STSTSDSDSA SKSTSVSDSD SASLSKSTST
STSDSDSASK STSVSDSDSA SLSKSTSTST SDSDSASKST SVSDSTSTSL SKSTSTSTSD
SDSASTSTSV SDSTSASLSK STSTSTSDSD SASTSVSDSD SASLSKSTST STSDSDSVST
STSVSDSDSA SLSKSTSTST SDSDSASKST SVSDSTSTSL SKSTSTSTSD SDSASTSVSD
STSASLSKST STSTSDSDSA SKSTSVSDSD SASLSKSTST STSDSDSAST STSVSDSDSA
SLSKSTSTST SDSDSASTSL SKSTSTSTSD SDSTSTSLSK STSTSTSDSD SASKSTSVSD
SDSASLSKST STSTSDSDSA SKSTSVSDSD SASLSKSTST STSDSDSASK STSVSDSESA
SLSKSTSTST SDSDSASTST SVSDSTSVSL SKSTSTSTSD SDSTSTSLSK STSTSTSDSD
SDSASLSKST STSTSDSDSV STSTSVSDSD SASLSKSTST STSDSDSAST STSVSDSGSA
SLSKSTSTST SDSDSASKST SVSDSDSASL SKSTSTSTSD SDSASTSTSV SDSTSASLSK
STSTSTSDSD SASTSTSVSD SDSASLSKST STSTSDSDSA SKSTSVSDSD SASLSKSTST
STSESDSASK STSVSDSDSA SLSKSTSTST SDSDSASTST SVSDSDSASL SKSTSTSTSD
SDSASKSTSV SDSDSASLSK STSTSTSESD SASTSTLVSD STSVSLSQST SVDKDSTAKG
STELVNVASL SISASQSSSL SASTSTSIEK SESTSTSGSN STNASLSSSS SLSTSASTSV
SEVTSVTHSE NDLSASNDRD TSGSVSQFAS ENTSLSDSAS ISGEVSSSTS ASTSKSSSLS
ASALHDKHVS ESTSASLSSG DSSRASASVS TSLSESDSAL IDSESISVSE HTSTLQSGSH
SLSQQQSAEL SQSEQTSQSQ RISTSASVSA MKSESAAKVS ESLSTSQSKV DSQSQSVSES
ASNSRVSRDS KSTSASMHRS LSESVSQSMS LIDQSESDST SISISTSISD EDSMLYSMSD
SASISTKASS SMSTSTSEEH ANSHSQSEST ASVEVSQEMS ASASTSKSES QSESVSVSNE
ESNISSMQES FVESAKASRS ASMSVAKSEA SESQLLSESN ASVSQSASTS SKASASTSES
ISTSLSVSEA THGKPRNHSE SASASQLLEE NESLSDSAST SVEDSESASA SLSVYQSQSA
SALKSTHASE KASVNTSANA SKRASASTSI SNSKSKVIAS ESKSTSISTY ESLSISTSKE
QSTRVSVSES TSTSKVKSES DSASTSTSES ISISANRSGY TSSKRSVQMS EAQSTSDSLS
VMQSEGSVSV SQSLSISDKT SQSLSESISH SESDSDSNSV SISQETSEQH SVSDSDSMSI
SESESIAYSQ SASESESTSI AKSDSISNSL SVSLSESESE ASTSASVSTS ESTSVKGSLS
TSILNSQSAS THQSTEASQS TSTSKVEEAS LSDSASVSDS QSLSMSHEKS QSASTSKSTS
LSKTISESES VSASTSTSEA VSTEASEFVS AVDSLSQVTS NGSTTKEDAS TFVSTVDSLK
DKASNNGTPS EFASAVKSTH ASVSVSASES TSASTSTSEA VSTEASEFVS AVNSLSEATS
NGSTTKEDAS TFVSTVDSLK DKASNNGTPS EFASAVKSTH ASVSVSASES TSASTSTSEA
VSTEASEFVS AVDSLSQVTS NGSTTKEDAS TFVSTVDSLK DKASNNGTPS EFESVVKSVH
GSMSASASAS TSASTSASTS TSEAASAEAS ELESVRKSLS NGASNGSTAR EGASTFVSTV
DSLKDKASNN GTASEFESVV KSVHGSTSAS ASASTSASTS ASESASTEAS EFVSAVASLS
SSAWNGSTTG EGASTFVSTV DSSKDSASDK ASPSESESVV KSVHGSTSTS ASVSASASTS
ASTSTSEAVS TEASEFVSAV NSLSSEASNG STTREGASTF VSTVDSLKDK ASNNGTASEF
ESVVKSVHGS MSTSASVSAS ESTSASTSTS EAVSTEASES ASISVSMSVS ASTSASMSVS
VSNSVSVSDS ISVSASTSEP NSVSTSMSSS LSTSASTPSE ITSSSSSSDS ATVQKVVSKD
EQHATNKVEK LPDTGQSTTQ TGLLGGVGAL LTGLGLLKKS RKQKDEETSS HE SEQ ID
NO: 13 atgaaaaagtctagaaaaaagcgtatcgattttttacctaaccgtcaaaatcgatatgcg
atacgtcgtttttcagtaggcactgcgtcaattctcgttggagcaacattaatttttgga
attcattcaaatgatgcatcggcagcagtagaagacgcaacatctcaagaagcaggaaca
actaacgaaaattcaaatagtacagaagaagcaacaacaaacgaaagtacaactgttgaa
gcaccaacaagtgaagaagcaacaacggaagagcaatcagtagaggcgccaacaagtgaa
gaagtaacaacggaagagcaatcagtagaggcaccaacaagtgaagaagtaacaacggaa
gagcaatcagtagaagcgccaacaagtgaagaagtaacaacggaagagcaatcagtagaa
gcgccaacaagtgaagaagtaacaacggaagagcaatcagtagaggcaccaacaagtgaa
gaagtaacaacggaagagcaatcagtagaggcaccaactagtgaagaagtaactacggaa
gagcaatcagtagaagcaccaacaagtgaagaagcaacaacggaagagcaatcagtagaa
gcaccaacaagtgaagaagcaactacaaaaactcctgtaaaagaagaaacatcctcaaca
caagaaaattcacccacgactacactagaagaacaattttcaaatgaattcaatcagtta
acatctacagaagataaaacaaactacacacgtgaatatttaactcaaaacacaaatctt
tcggcagaacaagtggaagcaacagttgaacgcttgaatttaagtcaagaaaatgtaaca
gcccaagatatctatttcgcattacttaaagatttagctgatcaacaagatgccttatta
ccacgtgtaacacttttggccgctagagattctgagctcacaaacgaagcgtctatcgct
ttaactgaaaatagtccaatgttccgcgcagcattagcgaatagtccttctggcaatgat
gtggtgtcagaagaagataatattattgtggctgatgcactcgcaaatggatacatcaat
tcacaaacagatgcaacaaatgcggcaaatacattgtctggtcgtgcatgggttgtggat
acagggacaccagcgacaatgtcaaacggcttaacagctgttccagaaggcacaaaagtc
tacatgcaatggattgatacagatggcgcggtttcaccagtgtatcaagcaagcacaaca
aataaattgagttcaagtggtggtagccaagtaggtccaggtgcatatgcatttgattta
cgtgaagcatggatagactcaaatggcaaagcgcacagatatgaagcgtcaagtggccaa
tattatcgtttatggattgatgactacaaaacagtagatgggaatacggcaaccatgtta
cgccaagcaggtggtttcttccctggttcatatgttaattcggtgacaggtaacaatatt
ggtcaattcccacttatcggaacgaacatgcaacgtacaggtatctttatgggtgtgata
ccaacgaacgattacatgactacagatacaagcaattggattcaagataatgaaggacct
atttcaaacccagcagtaacgagcacaagtgaatttgtcagtggtaaagtatggtctgag
acaggttcaggtgactatgcgaactctgcgacaggtccaaactttaactcaggtgatatt
gcacgtgaaggttatcaagttgtcatgtcttcattaacaagtgctggtgcccaagcgtat
aaagcacaagtcgaatcgttgccaacagaccaacaagcggcagcagcacaccaattattc
aaagaccacccagaatttatttctgcgacagtgacgggtaaaactgatgcaaacggtgcg
tatacattacgtttcccttcaggctcattgagtaaagattatctttatggttatgtgatg
gataataagggcaacttggttaagggctattcatcattcacgtcacctttattccgttcg
cctaacagtaacttatctttcgcgccacaaacagcgccatatcatagaccagccaaaaat
gcttgggtgaatgtgaactttgcgcttgtagaaacaattgaaacaagtatagacatcacg
aactttgatgtgacagccaacccagcgcaacgtggtgatacggctatcattgatgtgact
tctacagcattgtcaccattacctacgcatgttgagtggagagattcaaaagggaatgtc
gttcaaaaaagtggagatgtcactacggtagaagaagctgaaacggcaggcacatttact
attcctgatgatgcgaaaacaggtgaaatctatacagtttatattgtttcaggaggcaat
gaagttgcagcagactcactgattgtccaagtgcaagaaaatgcggcaacctatgaacct
gtatatccaacaacaacagttgaacaagaccaaactgtaacaattcctacacctacaaat
gaagatggtttagcattaccagacggaacaaagttcgaaggtggcaacaatgtacctgaa
tgggcaactgtgaatgaagatggttctatttcaatttcaccaaatcaagatgtggaaaaa
ggtaactataatgtgcctgttgtcgtcacatatccagatggttcaaaagaaacagtattt
gcaccagttttagttcaagaagctgttccaactgcagaacaatacgatccaacaattgaa
acaattaataaggaatatggtactactgcaacagaagatgaaattaaaggcgcaatcaca
attccggattacccaacagatggagatcaaccaacaatcacgattgacgacccaactcaa
attccaaatggaacagaagaaggcacagtgaatgtaggtgtcactgtcacttatccagat
ggttcaacagacaaattaacagtaccagtcgttacaggtaagcaagcggataacgataag
tacacaccagaaacaacaccaattacgaaagacttcggtacaggtgtaacagaagacgaa
gtgaaaggtgcagtcactgttccggattacccaacagatggagaccaaccaacaattacg
attgacgacccaagtcagttgcctgatggttcaaaagaaggaacaacggatgtcgacgta
acagtggaatatccagacggcacaacagatcacatcacagttccagtgactgttggaaag
caagcggataatgataagtacacaccagaaacaacaccaattacgaaagacttcggtaca
ggtgtaacagaagacgaagtgaaaggtgcagtcactgttccggattacccaacagacggt
gaccaaccaacaattacaattgatgatccaaatcaattaccggacggttcacaagaaggt
acgactgatgtaaatgtaacagtggaatatccagatggcacaacagatcacatcacagtt
ccagtgactgttggaaagcaagcggataatgataagtacacaccagaaacaacaccaatt
acgaaagacttcggtacaggtgtaacagaagacgaagtgaaaggtgcagtcactgttccg
gattacccaacagatggagatcaaccaacggttacaattgatgatccaaatcaattaccg
gacggttcacaagaaggtacgactgatgtaaatgtaacagtggaatatccagacggcaca
acagatcacatcacagttccagtgactgttggaaagcaagcggataatgataagtacaca
ccagaaacaacaccaattacgaaagacttcggtacaggtgtaacagaagacgaagtgaaa
ggtgcagtcactgttccggattacccaacagacggtgaccaaccaacggttacaattgat
gatccaaatcaattaccggacggttcacaagaaggtacgactgatgtaaatgtaacagtg
gaatatccagatggcacaacagatcacatcacagttccagtgactgttggaaagcaagcg
gataacgataagtacacaccagaaacaacaccaattacgaaagacttcggtacaggtgta
acagaagacgaagtgaaaggtgcagtcactgttccggattacccaacagatggagatcaa
ccaacggttacaattgacgatccgagtcagttaccagatggctcacaagaaggcacaaca
gatgtgaatgtaacagtggaatatccagatggcacaacagaccacatcacagttccagtg
actgttggtaagcaagcagataacgataagtacacgccagaaacaacaccaattacgaaa
gacttcggtacaggtgtaacagaagacgaagtgaaaggtgcagtcactgttccggattac
ccaacagatggagaccaaccaacaattacaattgacgatccgagtcagttaccagacggt
tcacaagaaggtacgactgatgtaaatgtaacagtggaatatccagatggcacaacagat
cacatcacagttccagtgactgttggtaagcaagcagataacgataagtacacaccagaa
acaacaccaattacgaaagacttcggtacaggtgtaacagaagacgaagtgaaaggtgca
gtcactgttccggattacccaacagatggagaccaaccaacaattacaattgacgatccg
agtcagttaccagacggttcacaagaaggtacgactgatgtaaatgtaacagtggaatat
ccagatggcacaacagatcacatcacagttccagtgactgttggaaagcaagcagataac
gataagtacacaccagaaacaacaccaattacgaaagacttcggtacaggtgtaacagaa
ggcgaagtgaaagattcaatcacaattcccggttacccaacagatggagaccaaccaaca
attacaattgacgacccaagtcagttaccagatggttcacaagaaggtacgactgatgtc
gatgtaacagtggaatatccagacggcacaacagatcacattacagttccagtgactgtt
ggaaagcaagcagataacgataagtacacaccagaaacagaaggtgtcaacaaagatcat
ggtacgtcagtaacagaagatgaagtgaaaggtgcagtcactgttccgggatacccaaca
gatggagatcaaccaacggttacaattgatgatccaagtcaattgccggacggttcacaa
gaaggtacgactgatgtaaatgtaacagtggaatatccagacggcacaacagaccacatt
acagtcccagtaactgttggtaaacaacctactaaagataacggggctacagataatgat
ggcgacatgaatcaaggcacagatgaaggaaatagtgctactgatcatggcgacaatgta
aaacaagattcaaacggaaactatacgccggttgaacaacgtgacaatcatgcgacttca
cctgcaacagatatggatccaatgccaagcaatagccaaacaacttttgatggcataaat
gcaaaaggttcaacttcagagaaagcaaaccataaacaacagtctgagcaattaccagac
acaggtgaaagcaatacacaaaatggtgcacttttaggcggattatttgcagcacttgga
ggcttattcttaatcggcagacgtcgtaaagaaaaagaaggcaaataa
The protein sequence translated from SEQ ID NO 13 is designated SEQ
ID NO: 14 and is shown below:
TABLE-US-00007 SEQ ID NO: 14 MKKSRKKRID FLPNRQNRYA IRRFSVGTAS
ILVGATLIFG IHSNDASAAV EDATSQEAGT TNENSNSTEE ATTNESTTVE APTSEEATTE
EQSVEAPTSE EVTTEEQSVE APTSEEVTTE EQSVEAPTSE EVTTEEQSVE APTSEEVTTE
EQSVEAPTSE EVTTEEQSVE APTSEEVTTE EQSVEAPTSE EATTEEQSVE APTSEEATTK
TPVKEETSST QENSPTTTLE EQFSNEFNQL TSTEDKTNYT REYLTQNTNL SAEQVEATVE
RLNLSQENVT AQDIYFALLK DLADQQDALL PRVTLLAARD SELTNEASIA LTENSPMFRA
ALANSPSGND VVSEEDNIIV ADALANGYIN SQTDATNAAN TLSGRAWVVD TGTPATMSNG
LTAVPEGTKV YMQWIDTDGA VSPVYQASTT NKLSSSGGSQ VGPGAYAFDL REAWIDSNGK
AHRYEASSGQ YYRLWIDDYK TVDGNTATML RQAGGFFPGS YVNSVTGNNI GQFPLIGTNM
QRTGIFMGVI PTNDYMTTDT SNWIQDNEGP ISNPAVTSTS EFVSGKVWSE TGSGDYANSA
TGPNFNSGDI AREGYQVVMS SLTSAGAQAY KAQVESLPTD QQAAAAHQLF KDHPEFISAT
VTGKTDANGA YTLRFPSGSL SKDYLYGYVM DNKGNLVKGY SSFTSPLFRS PNSNLSFAPQ
TAPYHRPAKN AWVNVNFALV ETIETSIDIT NFDVTANPAQ RGDTAIIDVT STALSPLPTH
VEWRDSKGNV VQKSGDVTTV EEAETAGTFT IPDDAKTGEI YTVYIVSGGN EVAADSLIVQ
VQENAATYEP VYPTTTVEQD QTVTIPTPTN EDGLALPDGT KFEGGNNVPE WATVNEDGSI
SISPNQDVEK GNYNVPVVVT YPDGSKETVF APVLVQEAVP TAEQYDPTIE TINKEYGTTA
TEDEIKGAIT IPDYPTDGDQ PTITIDDPTQ IPNGTEEGTV NVGVTVTYPD GSTDKLTVPV
VTGKQADNDK YTPETTPITK DFGTGVTEDE VKGAVTVPDY PTDGDQPTIT IDDPSQLPDG
SKEGTTDVDV TVEYPDGTTD HITVPVTVGK QADNDKYTPE TTPITKDFGT GVTEDEVKGA
VTVPDYPTDG DQPTITIDDP NQLPDGSQEG TTDVNVTVEY PDGTTDHITV PVTVGKQADN
DKYTPETTPI TKDFGTGVTE DEVKGAVTVP DYPTDGDQPT VTIDDPNQLP DGSQEGTTDV
NVTVEYPDGT TDHITVPVTV GKQADNDKYT PETTPITKDF GTGVTEDEVK GAVTVPDYPT
DGDQPTVTID DPNQLPDGSQ EGTTDVNVTV EYPDGTTDHI TVPVTVGKQA DNDKYTPETT
PITKDFGTGV TEDEVKGAVT VPDYPTDGDQ PTVTIDDPSQ LPDGSQEGTT DVNVTVEYPD
GTTDHITVPV TVGKQADNDK YTPETTPITK DFGTGVTEDE VKGAVTVPDY PTDGDQPTIT
IDDPSQLPDG SQEGTTDVNV TVEYPDGTTD HITVPVTVGK QADNDKYTPE TTPITKDFGT
GVTEDEVKGA VTVPDYPTDG DQPTITIDDP SQLPDGSQEG TTDVNVTVEY PDGTTDHITV
PVTVGKQADN DKYTPETTPI TKDFGTGVTE GEVKDSITIP GYPTDGDQPT ITIDDPSQLP
DGSQEGTTDV DVTVEYPDGT TDHITVPVTV GKQADNDKYT PETEGVNKDH GTSVTEDEVK
GAVTVPGYPT DGDQPTVTID DPSQLPDGSQ EGTTDVNVTV EYPDGTTDHI TVPVTVGKQP
TKDNGATDND GDMNQGTDEG NSATDHGDNV KQDSNGNYTP VEQRDNHATS PATDMDPMPS
NSQTTFDGIN AKGSTSEKAN HKQQSEQLPD TGESNTQNGA LLGGLFAALG GLFLIGRRRK
EKEGK SEQ ID NO: 15
atgacagaacgaaaatccccttcatctcaaaacatgcgtcatcgtttagtcaaagctggt
actgtccttttattggttggtagtggactgcaaatgccttcaacattgtcacacgaaatg
acagcgatagctcagacagatgcgactgatgatttgaaaacattacgtgaaaatgcagat
aaaaaagtgaaagcgttacaatatttaaatacggattataaaaatgaatttcttgcgtta
attcgtgaatatgatacgtcgtcaaaaaatattgaagtggttgttgacgaagcagaagca
gccaatcgtctagctcatgacgctcaatcggacgatgaaatacaacctgaattagatgcc
attgatgaaaaaattagcgcgttaaaggcaaaggttgatgaaggtcaacgagaatcaact
gaagcgcgtcaagatgtaacgtcaacagagacaaagagtgctgaatcagaaggaagagag
ccatccactgaaggcgagagcaaagtaaaggagtcatcttcagcacaaacgattgtagca
cctcatcatggtcaacaagatgtgagcgcactgaaagaccatattaagaacgatgtcgat
acacttaaacaagactatgcaacgcaagacaagcaagtgacaccactccagggcattgac
agtgcaatcacacgcattgaccatttcgtttcagaaagcgtggatcacaagtctgacaat
tattttgaagaaaaacgtcaacatttacaaaactttgaacaagacattaaaaaacgtacg
gacatttctgggactgagaaggcgactttgcttgatgatgcgaaaacggtagccaaccaa
ctgaacgcgcaaaatgatacgattttaactgaacttcaacagcatgacgataaacgtgca
gcagttgaatcgatattaggtgagatttttaatgcacaagaagcggctgaacgtgcgaaa
cagatagatgttaaaggtaaaacagatcaacaattggcaaacgaaattcatcaacaagcg
gacggacttatcaaaacgtcgagtgatgatttattgttaggaatgttggaaaataattca
aatacacaaggtctagtggaaagcattttacgaacacgctttgacaaacaagaagcgcac
aaaattgccggcgaaatcatgcaaggcaagccttcaaatacagcgatactcgaccgcttg
aaagaccattttaaagcgaatggtaaggcgagtggagatgatattttaaatgcgttaatt
aataatacggatgcagatgctgaagtgattgaatcaattctagggggccgtcttaatgca
gaaaatgcaaaattgattgccgatcgtgtacagcaagataaaaagaagacacatcaaaac
ttaaaggcgattgaagacgaacttagtgcgcaagcgaatcgattgttaacgttacggaag
caattgcaacaaatccgtcataatacgcaaacagatatgaatgacttgtttgcaccactg
cgtcgtattgcaaatattctcggtggtggtttaaatcgtgacgacattcactcttcaggt
cgtacgaatgacaaattgcagcaactgttaaatcgtgatcattcgttgttaggtcgtggt
ggtgatttattcaaacatgattttgcgccaaagccgaatatcgatccatatcaagcgatt
aatagtcaaacggcatcagaaggttttttagatggtttatttgatcaaaatggcgatttc
aatttaccgaatacaggtgaaatagtgaagcggacttggctaccgttgggtattttagtc
gttgcaatcggtgtactgatcttaacggtgagatttcataaaaaaacacgcaaacaataa
The protein sequence translated from SEQ ID NO 15 is designated SEQ
ID NO: 16 and is shown below:
TABLE-US-00008 SEQ ID NO: 16 MTERKSPSSQ NMRHRLVKAG TVLLLVGSGL
QMPSTLSHEM TAIAQTDATD DLKTLRENAD KKVKALQYLN TDYKNEFLAL IREYDTSSKN
IEVVVDEAEA ANRLAHDAQS DDEIQPELDA IDEKISALKA KVDEGQREST EARQDVTSTE
TKSAESEGRE PSTEGESKVK ESSSAQTIVA PHHGQQDVSA LKDHIKNDVD TLKQDYATQD
KQVTPLQGID SAITRIDHFV SESVDHKSDN YFEEKRQHLQ NFEQDIKKRT DISGTEKATL
LDDAKTVANQ LNAQNDTILT ELQQHDDKRA AVESILGEIF NAQEAAERAK QIDVKGKTDQ
QLANEIHQQA DGLIKTSSDD LLLGMLENNS NTQGLVESIL RTRFDKQEAH KIAGEIMQGK
PSNTAILDRL KDHFKANGKA SGDDILNALI NNTDADAEVI ESILGGRLNA ENAKLIADRV
QQDKKKTHQN LKAIEDELSA QANRLLTLRK QLQQIRHNTQ TDMNDLFAPL RRIANILGGG
LNRDDIHSSG RTNDKLQQLL NRDHSLLGRG GDLFKHDFAP KPNIDPYQAI NSQTASEGFL
DGLFDQNGDF NLPNTGEIVK RTWLPLGILV VAIGVLILTV RFHKKTRKQ SEQ ID NO: 17
atgttaaaaaaattaattgttacaggtttgattgctacagcggcgacacaagtttatgcg
catgacacgcaagcggcggaaaagggtgctacagatgctccgaatgtgatggttaaggat
gaggcgaaaaaagaagtgacaccgataatccataaaccgacttgcatttacccgcatcta
gaaggcgaagatgatgctgcgtatttaaaacgtatggcaacgaatccaccagaaggcgca
gtgccgtacggtgtattgaataaagatggatcgattacagaaccgaatacaaatccacat
tttgatgttttaaaaattgaagatccaaatgcgatgaaagatttggttgatacaccggca
gatgatcaagatacggtaccgagtgatttacaaattgaaccaccagcattaataggacca
gctactaaacatacggatggtacgggagacgcaaaatctaatgatgaccacaaagtaaca
aaatcttcgggagcgtcagcccaagatatgaagaaaaaagacgtgacaacacaaactgca
caaccaaaagcagataaaaagatggcgactgcaaaagtagcaccagcgaaacaacaagat
aaagcagccaaaatgttaccagcagcaggggaaccacaagtgaatgcaatcagtcaaaca
gcacttgcactttcaatgatcgcattaggtgtcatcgcgttctttacacgacgacgcaaa
acaaattaa
The protein sequence translated from SEQ ID NO 17 is designated SEQ
ID NO: 18 and is shown below:
TABLE-US-00009 SEQ ID NO: 18 MLKKLIVTGL IATAATQVYA HDTQAAEKGA
TDAPNVMVKD EAKKEVTPII HKPTCIYPHL EGEDDAAYLK RMATNPPEGA VPYGVLNKDG
SITEPNTNPH FDVLKIEDPN AMKDLVDTPA DDQDTVPSDL QIEPPALIGP ATKHTDGTGD
AKSNDDHKVT KSSGASAQDM KKKDVTTQTA QPKADKKMAT AKVAPAKQQD KAAKMLPAAG
EPQVNAISQT ALALSMIALG VIAFFTRRRK TN SEQ ID NO: 19
atggtagaatataaaaaagaacatagcgtaaagcgactattaaaattaggaatcggttca
acgagtattttatgtgttgtatcacctcttttattaacacatgacgttgttcaagcagca
gatatcaataacaggatgccagctttgaatacattgaagaccacttcttcatatgatcaa
agggcacacatggatgaattacgaaacgccattacttcagatagtgacactactcaaaca
ccatcattcaatgagataactgtgtcttcaactaatgaaacggatgcagcgtcaacggaa
aatgtgaacccgagtgatgaggtcccggcaaaggatgaaagtgaatcaacgacaccgagt
acagaacaagacacatctatagaagaaacgggtactgaagaagtgccatctcatgaagac
aatcatcacaacaccccaagtcaagaagagcaaccgtctccgcctgatcaaccaggaaca
aacaaagatgaagagagtggagaaaaaccgaataaagaaaatcatcggaagccgaatcaa
ccgaacaaagaccaaccttcaaaagatgagaataaaaaacctgacaaaggaaacaaacca
gcaccaccgtctaaaatgccaaatcgcccggatcaaaaggaagatggttcaaacaacacc
ccaccacctgccactgataacggtggaaacagtaatgacggtacaacaacgggtcccaat
ggtggaggtggcagtgaagcaagtccaccaccgaatgagcaaccgtcaaatggcaatgca
agcgatacccatcaaaacggttcagtttcaagcaccaatcattcgaatcagtatggtaca
tcggcttatgatgaatacgcaggtttattgaataataattataaatataatccattgttt
aaagaagaggttgcgcgtttaagtcaatttggaagtcaagatcaacatgatattgcaagt
ttgagtcgtaaagaacaattttctcaaaatgcatttttagatgacttgcaacaaagtaca
gattattttagatatcaatattttaacccgctttccacagagcaatactatcatcgttta
gataaacaagtattagcactcgttacgggggaatttggttcgatgccagatttcaagaaa
agtggtgataagtcattggttaataagcatcagcaagataaagtgaagaaaattgaacag
caaggagaaaatattaatacgcatcatatgaaaaatacgaaagaagatacaggaaaatca
ttaagttacaagccgatgatatatattggcattgtcatggtcggttttgtcggcctgatc
agtatgattttatggaaacgactgcatcatttttggaaataa
The protein sequence translated from SEQ ID NO 19 is designated SEQ
ID NO: 20 and is shown below:
TABLE-US-00010 SEQ ID NO: 20 MVEYKKEHSV KRLLKLGIGS TSILCVVSPL
LLTHDVVQAA DINNRMPALN TLKTTSSYDQ RAHMDELRNA ITSDSDTTQT PSFNEITVSS
TNETDAASTE NVNPSDEVPA KDESESTTPS TEQDTSIEET GTEEVPSHED NHHNTPSQEE
QPSPPDQPGT NKDEESGEKP NKENHRKPNQ PNKDQPSKDE NKKPDKGNKP APPSKMPNRP
DQKEDGSNNT PPPATDNGGN SNDGTTTGPN GGGGSEASPP PNEQPSNGNA SDTHQNGSVS
STNHSNQYGT SAYDEYAGLL NNNYKYNPLF KEEVARLSQF GSQDQHDIAS LSRKEQFSQN
AFLDDLQQST DYFRYQYFNP LSTEQYYHRL DKQVLALVTG EFGSMPDFKK SGDKSLVNKH
QQDKVKKIEQ QGENINTHHM KNTKEDTGKS LSYKPMIYIG IVMVGFVGLI SMILWKRLHH
FWK SEQ ID NO: 21
gtgattacaaataaaaatatatatagtattcgaaagcataaacttggcgtggcatcattc
ttattggggacattatttgttgtagggcatgcaaataatgctgaagcttcagaagtgagc
gcaacaacacaagaacataatgtcgagactgagcaaacaaaaactgagggcgaactaaca
actgaggtagcacaacaagcagtcagcgaatcagcacctatagctgaaaacatgcagaaa
acaacatcagtggcaagtgaaaatgcgaaagaggttacagcttctgatagcacacaagaa
gtcacaaaaactgaagcaaaagatacagcaacaatgaaagattcagaaattgcacaacct
gtatcagaagtgaataaacctgttactcaaacagctgcacccgtagcagaaccatcaaca
gcaaacaaacaaacttcaccacgacaagtacaagaacttactgcaccaatggacacaaaa
gtaattaatgtagaaaacggaacagatgtgacaagtaaagtgaaagttgaaaaatcgtca
attacagggcatcagaataaagataaaacatatcatcaatcgaacactgtaaatccacat
aaagctgaacgtgtgacattaaattatgattggtcatttgaaaatggaattaaagctggt
gattattttgacttccaattaagcgataatgtcgatacaaatggaatatcaacaataaaa
aaagtcccacacattatggatagtcaaaatagcgaacaaattattgcttacggggaaatt
aatgaaaacaaccgtgtccgttaccgatttatggactatgtaaatcaaaaagaaaattta
aaaggtaaattgtcattaaacttatttattaaaccagataaagttcaagatgaaggaaaa
atcactgtcacttcacaattgggcaaggaaatgacaagtcaggaatttgacattaaatat
attgatggtgtaaaaagcccttcaggtatcacattaaacggtcgtcttgatgaattatca
aaagcagatcaatcatttacgcattattctatatttaaacctaagcataataacttaact
aatgtaactttaagaggcacagtttcaaataacgcacagcaaaatgaaaaaaatggtcaa
gttaatgtttacgaatatattggtcaaggagaattgccacaaagtgcttatgccaatgta
aatgatacgaagcagttcaatgacattactaagagtatgaaatcaatcaaaaataacagt
aatggctatgaaattacttttgacatgaacaaagacaatcatccttatatcatagtatat
caaggtcactttaacaataatgcaaaagactttgatttctcaacaaatgcgacaggttat
caaaatttaaatcaatcggaatatagttattattggccttacaattattcattcaattta
acatgggataatggtgttgctttctactctaataatgcaagtggggaagggaacgacaaa
cctgtaccgccgacttatggatatagtccgacagtaaatacaattcaagatactcatgcg
gattatcctgtaatgactttccaacaacctggaactctagaggagacagaagacagtatg
ccaatcactacacttaccgaatctggtgaggatcgtggtgaaaatacttctccaattatc
gagacaacagaagattcacagcctgttgagtttgaagaagagacaaatcatggcattcaa
gacgtgacacttcatgcagatgctgttgattttgaggaagaaacaaaccatggtgaacaa
gacacggtacaccactctgatgtcgttgaatacgacgaagatacgacaactggcatgtta
acaggtgccatttctgaccatacaacagaagaaggcacgatggagtacacaactgatggc
ttattgattgagtttgatgatgaaatgaatcctaatgtgagcggtcagtacgatgacatc
acaacggatacgatagaggaatcatctcatattgacacattcactgaacttgaatctgaa
tttggtcaacatgacggtatagtgacatttgaagaagatactatcgttgagaagccgaaa
acagaaaagggtaaccgagtaccacttgtaattgatttatcaacaccaaaacataaccat
cagttcaatattcaacctaccgatccaaatattgatacctctgctacgtatcgaattggc
aattttgtatggcgcgatgaagatcacaatggcgtacaaaatgatggtgaacatggtctt
gaaggtgttcttgtcacacttaaaacagctgatggtgtcgttttaaatacaacgacaagt
gatgccaatggacactaccagttcactaatgttcaaaaaggaaaatatattgttgaattc
actacacctgaaggttatgaagcaacaagcaaacatactacagcgaatactgaaaaagac
tctgatgggttaatcgcaaatatcgatgttactcaagatgatatgtcaatcgatgctggt
ttcttcccgttagaaaactggaatcctcagccagagccgaaaaaccctgatgatagagag
aaaccggcacctgagcaacctgatgtacctcagccagaaccgaaaaaccctgatgataga
gagaaaccggcacctgagcaacctgatgtacctcagccagaaccgaaaaatcctgatgat
agagagaaaccggcacctgagcaacctgatgtacctcaaccagagccgaaaaatcctgat
gataaagagaaaccggcacctgagcaacctgatgtacctcaaccagagccgaaaaatcct
gatgataaagagaaaccggcacctgagcaacctgatgcacctcaaccaaagccgatgctc
ccaggtgaaaaggtgaaacccaaaccaactcatcccggtgaagctatgcaaacaacacct
caggacaaatcaacatctcaaacagatgaagcacttcctaaaacaggtgaatcatcatca
caatcatctgctttaatcttcggtggtttactcagtctattaggacttggtttattacgt
cgatcatctaaacaaaaccgttcttcaatgaaataa
The protein sequence translated from SEQ ID NO 21 is designated SEQ
ID NO: 22 and is shown below:
TABLE-US-00011 SEQ ID NO: 22 VITNKNIYSI RKHKLGVASF LLGTLFVVGH
ANNAEASEVS ATTQEHNVET EQTKTEGELT TEVAQQAVSE SAPIAENMQK TTSVASENAK
EVTASDSTQE VTKTEAKDTA TMKDSEIAQP VSEVNKPVTQ TAAPVAEPST ANKQTSPRQV
QELTAPMDTK VINVENGTDV TSKVKVEKSS ITGHQNKDKT YHQSNTVNPH KAERVTLNYD
WSFENGIKAG DYFDFQLSDN VDTNGISTIK KVPHIMDSQN SEQIIAYGEI NENNRVRYRF
MDYVNQKENL KGKLSLNLFI KPDKVQDEGK ITVTSQLGKE MTSQEFDIKY IDGVKSPSGI
TLNGRLDELS KADQSFTHYS IFKPKHNNLT NVTLRGTVSN NAQQNEKNGQ VNVYEYIGQG
ELPQSAYANV NDTKQFNDIT KSMKSIKNNS NGYEITFDMN KDNHPYIIVY QGHFNNNAKD
FDFSTNATGY QNLNQSEYSY YWPYNYSFNL TWDNGVAFYS NNASGEGNDK PVPPTYGYSP
TVNTIQDTHA DYPVMTFQQP GTLEETEDSM PITTLTESGE DRGENTSPII ETTEDSQPVE
FEEETNHGIQ DVTLHADAVD FEEETNHGEQ DTVHHSDVVE YDEDTTTGML TGAISDHTTE
EGTMEYTTDG LLIEFDDEMN PNVSGQYDDI TTDTIEESSH IDTFTELESE FGQHDGIVTF
EEDTIVEKPK TEKGNRVPLV IDLSTPKHNH QFNIQPTDPN IDTSATYRIG NFVWRDEDHN
GVQNDGEHGL EGVLVTLKTA DGVVLNTTTS DANGHYQFTN VQKGKYIVEF TTPEGYEATS
KHTTANTEKD SDGLIANIDV TQDDMSIDAG FFPLENWNPQ PEPKNPDDRE KPAPEQPDVP
QPEPKNPDDR EKPAPEQPDV PQPEPKNPDD REKPAPEQPD VPQPEPKNPD DKEKPAPEQP
DVPQPEPKNP DDKEKPAPEQ PDAPQPKPML PGEKVKPKPT HPGEAMQTTP QDKSTSQTDE
ALPKTGESSS QSSALIFGGL LSLLGLGLLR RSSKQNRSSM K SEQ ID NO: 23
atggcatttgatggtatgtttacaagaaaaatggtagaagatttacaatttctcgtttct
gggcgtattcataaaatcaatcaaccggaaaacgatacaatcatcatggttataagacag
caacgccaaaatcatcaattgttgttgtcgattcacccgaattttgcacggattcacctc
actacaaaaaaatatgataatccatttgaaccgccgatgtttgcgcgcgtctttcgtaaa
catttagaaggtggacgtatccttgccattcgccaaatcggaaatgaccgtcgcatcgaa
atggacgtggaaagtaaagatgaaattggtgacacgattcatcgtacagtgattttagaa
attatgggcaaacatagtaatctcattctcgttaatgaagaacgtaaaattttagaaggt
tttaaacaccttacaccaaatacgaatcaatttagaaccgtgatgccaggttttcaatat
gaagtgccgccaacacaacataaacagaacccttatgcatatactggtgcgcaagtgctc
caacatattgatttcaatgcgggcaaaattgatcgccaactgcttcaaacgtttgaaggt
ttttcaccgttaatcacaaaagaaatcacatcaagacgccattttatgaccacacaaact
ttacctgaagcttttgacgaagtgatggccgaaacgaaagcgacaccccaaccggtattt
cataaaaataacgaaacaggtaaagaagacttttattttatgaagttacatcagttttac
gatgattgcgtcacatatgattcactccatgaactgctcgaccgtttttatgatgcacgc
ggtgaacgtgaacgcgtcaaacaacgtgcaaacgatttagtcaaactcgtccaacaatta
cttcaaaaatatcaaaataaattaagtaagctcgtcgatgaacaagcggggactgaagaa
aaagaaaatcaacaattgtacggcgagttaatcacagcgaatatttatcaactcaaacct
ggagatcgccagttagaaacagtaaattattatacaggagaaaacgtgactattccgtta
aatccacaaaagtcacctgctgaaaatgcgcaatactattacaagcaatacaaccgaatg
aaaacacgtgagcgcgaattgacccatcaaattactttaacggaagaaaatatcgcttat
tttgaaaatatcgagcaacagttgtcacacattcaagttcatgaaattgacgatattcgt
gaagaactagcagaacaaggctttatcaaacaaaagaaacagcagaaaaagaaaaagcaa
caaaaaatccagttacaatcctacgtttcgactgatggcgatacgattttagtcggtaaa
aataataagcaaaatgattatttaacgaataaacgtgcgcaaaaatcgcatttatggttc
catacaaaagatatcccaggaagccatgtcgtgattttaaatgatgcgccaagtgacaaa
acgattgaagaagcggcgatgattgcagcgtacttttcaaaggcggggcaatcgggacaa
attccagtggattatacaacaattcgcaatgtgcataagccgagtggcagtaaacctgga
tttgtaacgtacgataaccagaagacgctttacgcaacgccggattatgacatgattcgt
cgattgaaagctgaagaagcgtaa
The protein sequence translated from SEQ ID NO 23 is designated SEQ
ID NO: 24 and is shown below:
TABLE-US-00012 SEQ ID NO: 24 MAFDGMFTRK MVEDLQFLVS GRIHKINQPE
NDTIIMVIRQ QRQNHQLLLS IHPNFARIHL TTKKYDNPFE PPMFARVFRK HLEGGRILAI
RQIGNDRRIE MDVESKDEIG DTIHRTVILE IMGKHSNLIL VNEERKILEG FKHLTPNTNQ
FRTVMPGFQY EVPPTQHKQN PYAYTGAQVL QHIDFNAGKI DRQLLQTFEG FSPLITKEIT
SRRHFMTTQT LPEAFDEVMA ETKATPQPVF HKNNETGKED FYFMKLHQFY DDCVTYDSLH
ELLDRFYDAR GERERVKQRA NDLVKLVQQL LQKYQNKLSK LVDEQAGTEE KENQQLYGEL
ITANIYQLKP GDRQLETVNY YTGENVTIPL NPQKSPAENA QYYYKQYNRM KTRERELTHQ
ITLTEENIAY FENIEQQLSH IQVHEIDDIR EELAEQGFIK QKKQQKKKKQ QKIQLQSYVS
TDGDTILVGK NNKQNDYLTN KRAQKSHLWF HTKDIPGSHV VILNDAPSDK TIEEAAMIAA
YFSKAGQSGQ IPVDYTTIRN VHKPSGSKPG FVTYDNQKTL YATPDYDMIR RLKAEEA SEQ
ID NO: 25
atggtcaaaaaatttggttataaaacacctacaatcgttgcacttactttggctggaact
gcattttctgcacaccaagccaatgccgctgaacaagttgcacctgaaaaaacacctacg
aatgtacttgatgatcaatacgcattaaaacaagctgatgatgcgaaacaaacgacacaa
ggaacaacacttgcaggttcaaaagaatacaaggatccttcacaaattgatacgactcaa
gtcgatacagcagcacaaactgaaacgcccgtagaaggagggcaacaagacgcacaacaa
cctactacaactgatgaagcgacatcaacagatcatactgtatcaaaaggtacaaacgaa
agtgcatcacctgcaacagcttctatagatgaaggaacattaaacgcacaagtcaattca
gatgaaacggctactaaccgtacacaagacgtcactgaaaatgtgacaaaatatccttat
cattcaagtgaaatcgatacacatgaagacgcaactgtgtcaccagatacatatcatgca
ctggacacgcatgcgcaacaaccttcagcaatggatgtaagcgattcaacatcagcacaa
actgaagcgacgcaagtaaatacgtcaacaaatgtaaatgacaaagaggccgtttcgaca
acagaagatgcacctactacacaacttcaagcagctgtacaatctgaagccaacaaagaa
gcgaaggcaactactgaaacagctcaaaataaaacacctcaagttgaaaagaaagcaaca
gcaactcaaaatacagcacagttagcaacggggcatcaggatattactgacaaagtctca
aaacgcgtagcagtgacaaatgaaacgaaagcggatgccacaacagcgaaaacacaagca
cctacttcagtgacacatcaagctgatacacaagcaaaaacgataacagacaagaaggca
acaacttacagtgcacaaaccgcaactgaccaagacataaatgcgaatccggacggtcca
acacctccacgcgttggcggtaaagggggtccccctgcttcactttcactccaatcgact
ggtcaaacagcattccgttcagctgtcgctagtaaaccgagtgcatatcaacctaaagtg
aaatcgtctattaatgactatattcgtaagcaaaactacaaagtgcctgtatatgaagaa
gattattcaagttacttccctaaatacggttatcgtaatggtgtcggtaaacctgagggc
atcatcgtgcatgatacagcaaatgacaactctacaattgatggcgaaatcagttacatg
aaaagaaattatcaaaatgctttcgtacatggctttattaatggtcaacgtattgttgaa
acgcaacctacagattatttagcatggggtgcaggtgcgattgcgaatgaacgctttatt
catatcgaactcgttcatgttcacagtaaagaagatttcgcacgtcaaatgaacaatatg
gcagattatgcggcgacgaacttacaatattatggcctttctccagatagtgcggaatat
gatggtcgtgggacagtttggacacatgatgctgtttctagatttttaggtggtacagac
cataccgatccgcacggctatttaaaacaacatggttattcctttgatgcgttgtatgat
ttaatcaatgaaaaatatcaagtgaaaatgggttatgcctcacctgctaactcgtcttca
aaaccatcaacaaatactggcttaacagttaaaaacacaacaggtttcggccgtattaac
acaacaaatagcggtttatatacgaccgtttatgatcaaaaaggtaaagcgacgaatcaa
acgaatcaaacgttaaaagttacaaaagaagcgacgttaaatggcaacaaattctattta
atgagtgatgcaaaatctaatcaaacactcggttgggtcaaatcaaacgacgcaacatat
caagctgcccaagctgagaaaaaagtaacgaaaacgtatactgtcaaaccaggaacaaca
gtatatcaagtgccttggggtgcctcatctcaaacagtaggcaaagctccaggtacgtca
aaccaatcattcaaatcaacgaaagaacaaactgttgcgaaaacgaaatggctttatggg
acagttggcaaagtgacaggctggattaatgcaagtagtgttgtagcaaatgatcaaaaa
ccatcgacgaataccgcactaaaagtaacaactgacactggtctcggtcgcattaaagac
aaaaatagtggtttatacgcaacggtatatgataaaactggtaaaagcacttcagccact
aaccaaacattaaaagtaacgaaaaaagcaagtgtcaatggccaatcattctatttagta
tcagattatgctaaaggtacaaatgttggttgggtgaaacagtcagatgtcgaatatcaa
acaagtaaagccccttctaaagtgaatcaaaattatacgattaaatcgggtgcgaaattg
tatcaagtgccttggggtacaagtaaacaagttgccggtacagtgacaggtgctgcgaca
caaacatttaaggcaacacaatctcaaactgtaggtaaagcaacatacttgtatgggaca
gttggcaaattatctggttggattaattcaacagcattagcagctcaaaaaacaacaacg
aatgttactaaaacaatttctcaaatcggtcaactgaacacgaaaaatagcggtgtcaaa
gcttctatttatgacaaaacagcaaaagatgcatccaaatgggcaggtcaaacttataaa
attactaaaacagcttctgccaataacgaagactatgtattactgcaaaatagtacagga
ggcacgccactcggttggttcaatgttaaagacgtcacaacacgcaacttaggtgctgaa
acagctgttaaagggcggtacactgttaatagtaaaacatctggactctacgctatgcct
tggggtacaacgaagcaacgtgtcgatacattaaaaaatgccacaagtcgtttatttaca
gcttcaaaatcagttaaagtcggtaatgatacattcttattcggtacagtgaatcaaaaa
ttgggctggattaatcaaaaagacttaacagctgtagcagcaaaagttgcaaacatgaaa
actgcatcgaatagcgcagtcaaaggtgccgcaatcacaactttgaaaaaagtagaagat
tatgtgattacgaataaaaatggttattattacactaaagttggagattcaaaaacagct
ggtgctttaaaaggtttttatcaacaaatttttaaagtcgaaaaaacatctttactgaac
ggcattacttggtactatggcgcattccaaaacgggacgaaaggatggattaaagcagct
gacatacgttcatcattcattcaacatactgcggtcagtagcacattgaaagcagcactc
gataaacaaatggcgctgacttacccgcctcaagttcaacgtgtagccggtaaatgggtc
aatgcgaatcgtgcagaaactgaaaaagcaatgaataccgcagcaattgaaaaagatccg
actctcatttaccaatttttaaaacttgataaataccaaggtcttggcgtagaagaactt
aataaattgttaagaggcaaaggcattttagaaggtcaaggtgccgcatttaaagaagcc
gcacaaaaacacaatattaatgaggtttacttaatgtctcacgcatttttagaaacaggt
aacgggacttctcaattagccaatggcggtcacgtagataaaaataataaagtcgtaaca
aacggtaaaccgaagtattacaacatgttcggtatcggggcaattgatacagacgcttta
cgcaatggctttaaaactgctgaaaaatatggttggaatacggtcagcaaagcgattatc
ggtggcgcaaaattcatccgtgatcagtacatcggttcaggacaaaacacattgtatcgt
atgcgttggaatccagaacaccctgccacacatcagtatgcgactgatattaattgggca
aatgtaaacgcacaacgcatgaaatatttctatgatcaaattggtgaaacaggtaaatat
ttcgacgtcgatgtatataagaagtag
The protein sequence translated from SEQ ID NO 25 is designated SEQ
ID NO: 26 and is shown below:
TABLE-US-00013 SEQ ID NO: 26 MVKKFGYKTP TIVALTLAGT AFSAHQANAA
EQVAPEKTPT NVLDDQYALK QADDAKQTTQ GTTLAGSKEY KDPSQIDTTQ VDTAAQTETP
VEGGQQDAQQ PTTTDEATST DHTVSKGTNE SASPATASID EGTLNAQVNS DETATNRTQD
VTENVTKYPY HSSEIDTHED ATVSPDTYHA LDTHAQQPSA MDVSDSTSAQ TEATQVNTST
NVNDKEAVST TEDAPTTQLQ AAVQSEANKE AKATTETAQN KTPQVEKKAT ATQNTAQLAT
GHQDITDKVS KRVAVTNETK ADATTAKTQA PTSVTHQADT QAKTITDKKA TTYSAQTATD
QDINANPDGP TPPRVGGKGG PPASLSLQST GQTAFRSAVA SKPSAYQPKV KSSINDYIRK
QNYKVPVYEE DYSSYFPKYG YRNGVGKPEG IIVHDTANDN STIDGEISYM KRNYQNAFVH
GFINGQRIVE TQPTDYLAWG AGAIANERFI HIELVHVHSK EDFARQMNNM ADYAATNLQY
YGLSPDSAEY DGRGTVWTHD AVSRFLGGTD HTDPHGYLKQ HGYSFDALYD LINEKYQVKM
GYASPANSSS KPSTNTGLTV KNTTGFGRIN TTNSGLYTTV YDQKGKATNQ TNQTLKVTKE
ATLNGNKFYL MSDAKSNQTL GWVKSNDATY QAAQAEKKVT KTYTVKPGTT VYQVPWGASS
QTVGKAPGTS NQSFKSTKEQ TVAKTKWLYG TVGKVTGWIN ASSVVANDQK PSTNTALKVT
TDTGLGRIKD KNSGLYATVY DKTGKSTSAT NQTLKVTKKA SVNGQSFYLV SDYAKGTNVG
WVKQSDVEYQ TSKAPSKVNQ NYTIKSGAKL YQVPWGTSKQ VAGTVTGAAT QTFKATQSQT
VGKATYLYGT VGKLSGWINS TALAAQKTTT NVTKTISQIG QLNTKNSGVK ASIYDKTAKD
ASKWAGQTYK ITKTASANNE DYVLLQNSTG GTPLGWFNVK DVTTRNLGAE TAVKGRYTVN
SKTSGLYAMP WGTTKQRVDT LKNATSRLFT ASKSVKVGND TFLFGTVNQK LGWINQKDLT
AVAAKVANMK TASNSAVKGA AITTLKKVED YVITNKNGYY YTKVGDSKTA GALKGFYQQI
FKVEKTSLLN GITWYYGAFQ NGTKGWIKAA DIRSSFIQHT AVSSTLKAAL DKQMALTYPP
QVQRVAGKWV NANRAETEKA MNTAAIEKDP TLIYQFLKLD KYQGLGVEEL NKLLRGKGIL
EGQGAAFKEA AQKHNINEVY LMSHAFLETG NGTSQLANGG HVDKNNKVVT NGKPKYYNMF
GIGAIDTDAL RNGFKTAEKY GWNTVSKAII GGAKFIRDQY IGSGQNTLYR MRWNPEHPAT
HQYATDINWA NVNAQRMKYF YDQIGETGKY FDVDVYKK SEQ ID NO: 27
gtgtcgacagaaaaacaagatgatacacaagcaaaagcgaatgcactttctacagatgat
tcaacacctacaacagaacaatcaaaaagtgataccgaaccaacgcaaaatcaagaagtg
aatgaaaaagaagcaacacaagttgagcaaactccagataatgcatcatcagaatttaaa
gacagtgcagcacaagatgaaacaacatcgaaagacgctgacattgctcaaacaaaagaa
gcaaaaaatgaagcattgcaaagtgactcatcagcaaacctatcaaatcaagaagcagaa
aaagaaaacacaactaacagtgaatctcaagtaaatgaacaacctaaagcagatacaact
tctgattcacaagtttcaaatacacctcaacaagatcctacatcgacagtaccttcacca
gaaacatcagaagacaatcgaccttcaacagaattaaaaaatagtgaaacaactgcttct
caaacaactttaaacgaacaacctactgaatcaacatccaatcaaactgaaacgacaaaa
gcaccaacaaatacaacagtcgcaaacaaaaaagcacctgcacaattaaaagacattaaa
ggtacaactcaacttcgcgcagtcagtgcaagtcaacctactgctgttgcagctggtggg
acaaacgtaaatgacaaagtaacagcatcaaatatgaaaataactgaatcttatatcgag
ccaaacaactcaggaaacttttatttaaaaagtaactttaacgtaaacgggactgttaaa
gaaggtgactactttactgtaaaaatgcctgacactgtcaatacttttggtgacacgcgc
cattcacctgactttagagaaaaaattacaaatcaaaaaggtgaagttgtggctttaggt
gaatatgatgttgccaaccatactatgacatacacgttcactaatgtcgttaataattta
gaaaatgtgtccggttcgtttaacttgactcaatttatggatcgtaaagtggcaacagat
tctcaaacatatccattaaaatacgacattgcaggcgaatctttagatacacaaattaaa
gtgaattacggtcaatattacagtgaaggtgattctaacttaaaatcaatgatcacttca
gaagatcctaaaactggggaatatgatcaatacatttatgtcaacccattacaaaaaacg
gcaaacggtacagttgtaagagttcaagggttccaagttgatccaactaagagtaatggg
caagtgaaaccagatacaacgcagatcaagattttaaaagttgctgatggtcaaccactt
aatagtagtttcggtgtgaatgacagtgaatatgaagatgtcacaaaacaatttaatatt
gtttatcgtgataataatttggcagatatttactttggaaacttaaatgggcaacgctat
atcgttaaagtgacgagcaaagaaaatttggattctaaagaggatttaaacttgcgtgct
attatggccactcaaaaccgatatggtcaatataactatattacttgggataacgatatt
gtgaaaagctcttctggtggtacagccgacggaaatgaagcatcatatcaattaggcgac
aaagtttggaatgatgtgaataaaaatggtatccaagatcaaggtgaaactggtattgct
gatgtaaaggttactttaaaagatcttgatggcaacattttggatacaacttatacaaac
acgaatggtaaatatatctttgataatttaaaaaatggtaattatcaagtgggttttgaa
acaccggaaggctatgctgcaagtccatccaaccaaggtaatgacgcccttgactctgat
ggtcctacaaatgtacaagctgtcattagtgatgggaacaacttaactatcgaccaaggt
ttttaccaaactgaaacaccaacacacaacgtcggcgacaaagtttgggaagacttaaat
aaagatggcatccaagaccaaaatgaaccaggtatcgctaacgttaaggtcactttaaaa
gacgcggatggtaacgttgtggatacacgtacgactgatgataaagggaattacttattc
gaaaaagttaaagaaggcgaatatacaattgaatttgaaacgcctgaaggttatacaccg
acacaaacaggccaaggcagagtcagcactgactctaatgggacatcttcccttatttta
gtcgaaggtaacgatgacttaacaatcgatagcggtttctacaaagaacctgttacacac
aaagttggcgacaaagtttgggatgacttaaataaagacggtatccaagatgacaatgaa
ccaggcatctctgacgttaaagtcactttaaaagatgcggatggtaacgtcgtagataca
cgtacaactgatgctaacggtaactatttatttgaaaacgtgaaagaaggcgactatacg
attgaatttgaaacgcctgaaggttacacaccgactgttacaggtcaaggtacagctgat
aatgactctaacggtacatctacaaaagttacagttaaagatggcgatgacttaacaatt
gacagtggtttcactcaagttacacctgagccaccgacacataatgttggcgacaaagtt
tgggatgacttaaataaagacggtatccaagatgacaatgaaccaggcatctctgacgtt
aaagtcactttaaaagatgcggatggtaacgtcgtagatacacgtacaactgatgctaac
ggtaactatttatttgaaaacgtgaaagaaggcgactatacgattgaatttgaaacgcct
gaaggttacacaccgactgttacaggtcaaggtacagctgataatgactctaacggtaca
tctacaaaagttacagttaaagatggcgatgacttaacaattgacagtggtttcactcaa
gttacacctgagccaccgactgaacctgaaaaccctagtccagagcaaccttctgaaccg
ggtcaacctgaaaatcctagtccagagcaaccttctgaaccaggtcaacctgaaaatcct
agtccagagcaaccttctgaaccaggtcaacctgaaaatcctagtccagaacaaccttct
gaaccgggtcaacctgaaaatcctagtccagaacagccttctgagccaggacaacctaaa
aatcctagtccagaacagccaaataatccaagtgtgccaggtgttcaaaatcctgaaaaa
ccaagcttaactccagtcacacaaccggttcattcaaacggcaataaagcaaaaccatct
caacaacaaaaagctttacctgaaacaggtgaaactgaatcacatcaaggtacattattc
ggtggtattttagctgctttaggcgcattactctttgcacgtaaaaaacgccacgataaa
aaacaatcacactaa
The protein sequence translated from SEQ ID NO 27 is designated SEQ
ID NO: 28 and is shown below:
TABLE-US-00014 SEQ ID NO: 28 VSTEKQDDTQ AKANALSTDD STPTTEQSKS
DTEPTQNQEV NEKEATQVEQ TPDNASSEFK DSAAQDETTS KDADIAQTKE AKNEALQSDS
SANLSNQEAE KENTTNSESQ VNEQPKADTT SDSQVSNTPQ QDPTSTVPSP ETSEDNRPST
ELKNSETTAS QTTLNEQPTE STSNQTETTK APTNTTVANK KAPAQLKDIK GTTQLRAVSA
SQPTAVAAGG TNVNDKVTAS NMKITESYIE PNNSGNFYLK SNFNVNGTVK EGDYFTVKMP
DTVNTFGDTR HSPDFREKIT NQKGEVVALG EYDVANHTMT YTFTNVVNNL ENVSGSFNLT
QFMDRKVATD SQTYPLKYDI AGESLDTQIK VNYGQYYSEG DSNLKSMITS EDPKTGEYDQ
YIYVNPLQKT ANGTVVRVQG FQVDPTKSNG QVKPDTTQIK ILKVADGQPL NSSFGVNDSE
YEDVTKQFNI VYRDNNLADI YFGNLNGQRY IVKVTSKENL DSKEDLNLRA IMATQNRYGQ
YNYITWDNDI VKSSSGGTAD GNEASYQLGD KVWNDVNKNG IQDQGETGIA DVKVTLKDLD
GNILDTTYTN TNGKYIFDNL KNGNYQVGFE TPEGYAASPS NQGNDALDSD GPTNVQAVIS
DGNNLTIDQG FYQTETPTHN VGDKVWEDLN KDGIQDQNEP GIANVKVTLK DADGNVVDTR
TTDDKGNYLF EKVKEGEYTI EFETPEGYTP TQTGQGRVST DSNGTSSLIL VEGNDDLTID
SGFYKEPVTH KVGDKVWDDL NKDGIQDDNE PGISDVKVTL KDADGNVVDT RTTDANGNYL
FENVKEGDYT IEFETPEGYT PTVTGQGTAD NDSNGTSTKV TVKDGDDLTI DSGFTQVTPE
PPTHNVGDKV WDDLNKDGIQ DDNEPGISDV KVTLKDADGN VVDTRTTDAN GNYLFENVKE
GDYTIEFETP EGYTPTVTGQ GTADNDSNGT STKVTVKDGD DLTIDSGFTQ VTPEPPTEPE
NPSPEQPSEP GQPENPSPEQ PSEPGQPENP SPEQPSEPGQ PENPSPEQPS EPGQPENPSP
EQPSEPGQPK NPSPEQPNNP SVPGVQNPEK PSLTPVTQPV HSNGNKAKPS QQQKALPETG
ETESHQGTLF GGILAALGAL LFARKKRHDK KQSH SEQ ID NO: 29
atgaagaaaacaatttcagtacttggtctagggctattagcaacattttttgtaagtaac
gaatcatatgccgcagaaacgattcaaaacaatacgtcatcaagtgaaacgaatcaaaat
tcagatcagacgccgttagatcattatattcgaaaagcagatggcacactggttgaaccg
aacgtgtacccacataaagattatgtagagaatgaaggacctttaccagagtttaaattt
caagttgactctaagaaagattcatctgatccaaatcaagcaccgttagatcattatatt
cgaaaagcggatggcacgttggttgaaccgaatgtatatccacacaaagattatgtcgaa
aatgaagggcctttaccagagtttaaatttatgtatgctgacaaacaaaatcatcatgac
caacagagtaaaaacaacaaggataagcagcgtgcaaattacagtgacaaaaagcataat
gatcagccgggtcatccaaaagcagtcacgccagctgtacaacatgataaagcagtcact
tcaaacgctactgtaaaagcattgccaaacacaggtgaatctgataaaacaacacaatta
ccaatcgtattatcattgttatctgtggggattttagttttattaaaattgagaaaataa
The protein sequence translated from SEQ ID NO 29 is designated SEQ
ID NO: 30 and is shown below:
TABLE-US-00015 SEQ ID NO: 30 MKKTISVLGL GLLATFFVSN ESYAAETIQN
NTSSSETNQN SDQTPLDHYI RKADGTLVEP NVYPHKDYVE NEGPLPEFKF QVDSKKDSSD
PNQAPLDHYI RKADGTLVEP NVYPHKDYVE NEGPLPEFKF MYADKQNHHD QQSKNNKDKQ
RANYSDKKHN DQPGHPKAVT PAVQHDKAVT SNATVKALPN TGESDKTTQL PIVLSLLSVG
ILVLLKLRK SEQ ID NO: 31
atgaaaagtaaatatgattttttacctaatagacttaataaattttctatacgaaaattt
actgttggtagtgtatcagtgctaataggagccactttattattcgggtttgtagaagga
gaagcatcagcatcagtaaaagaaggtcaacaaagtataaattctagtgagaaagaaagc
gccgatcctacagtagttgatttaattagtaagaaagaaacaaatttagatggactagat
gtatcaagagaagaaacgaccaaagtaccaataaatgaaaacaaaagaggtgaggaacaa
agtatttctgataaagctataacagaaaaagctgatacaccagtaagcaatttatcaagt
aaggaagttgaggagcaaggtgtttctgataaagctataacagaaaaagctgatacacca
gtaaccaatttatcaagtaaggaagctaaggagcaaggtgcttctgatagagttataaca
gaaaaagctgatacaccagtaagcaatttatcaagtaaggaagctaaggagcaaggtgct
tctgatagagttataacagaaaaagctgatacaccagtaagcaatttatcaagtaaggaa
gttgaggagcaaggtgtttctgataaagctatagagaaaatagctgatgcatcagctact
gatttgtcaagtaaggaagaagtagaacaagatatatctacacaaggtaaagtaaaatca
aaggaagcagtacaagtagaaagtagtcagttacaaaatttaaatagtgaaataaatgct
gaacctaatgaaattaaggcaatagatagaagttcaatattacctttaaatttaaatgat
gaagaaaataacaaaaaagttaataaagggactcgggttccagaagctacattaagaaat
gcctctaataaccaactcaatacacgaatgagatcagtgagtttatttagagttgctaga
ctaacagaaatcaatagaaatgttaatgataaagtaaaggtttcggatatcgacatcgca
atagccccaccgcatactaaccctaaaactggaaaagaagaattttgggcgacatcttct
tcagttttaaagttaaaggcaagctatgaattggataatagcatttctaaaggggatcaa
tttactattcaatttggtcaaaatattcgtccaggtggattaaatttaccaagaccttat
aattttttatatgataaggataaaaaattagttgcaactggccgttacaataaagaatca
aatacaatcacatatacatttacggattatgtagataaacatcaaaacattaaaggtagt
tttgagatgaatgcattttctagaaaggaaaatgctactactgacaaaacagcatatcca
atggatgttactattgcgaatcaaaaatatagtgaaaatattattgtagactatggtaat
aaaaagaatgctgctattatttcaagtacagaatatattgatttagatggtagtagaaaa
atgacaacatatattaatcaaaatggtagtaaaaattccatctatcgtgctgatatgcaa
attgatttgaacggttataaatttgatccatccaaaaacaattttaaaatttatgaagtg
gaaaatagcagtgactttgtggatagcttttcaccagatgtgagcaagttaagggatgtt
acgagtcaatttaatattcaatatacaaataataatacaatggcaaaagtggattttggt
actaacctttggaggggtaaaaaatatattattcagcaagtggcgaatatagacgacagt
aaattagtgaaaaatgcttcaatcaattatacattgaataaaatggattttaataataaa
agaacggtagaaacacataacaatacttattctacagtgaaagataaatcaacagcacta
ggtgacgtacaggaaagtcaatctattagtgagagccaatcagttagtgaaagcgagtca
ctaagtgagagccaatcaatcagtgaaagcgaatcattaagtgagagccaatcaatcagt
gaaagcgaatcattaagtgaaagtcaatcaatctcagagagcgaatcactaagtgaaagt
cagtcaatttcagaaagcgaatcattaagtgaaagccaatcaatctcagagagtgaatca
ttaagtgaaagtcagtcaatttcagagagtgaatcactaagtgaaagtcagtcaatttca
gaaagcgaatcattaagcgagagtcagtcaatttcagaaagcgaatcattaagcgagagt
cagtcaatttcagaaagcgaatcattaagtgaaagccaatcaatcagtgaaagcgaatca
ctaagcgagagccaatcaatctcagagagtgaatcattaagcgagagtcaatcaatctca
gagagcgaatcattaagtgagagtcaatcaatcagtgaaagcgagtcactaagtgagagt
caatcaatttcagagagcgaatcattaagtgaaagccaatcaatctcagagagtgaatca
ctaagtgagagccaatcaatctcagagagtgaatcattaagtgagagccaatcaatctca
gagagcgagtcactaagcgagagccaatcaatttcagagagtgaatcactaagtgaaagt
caatcaatttcagagagcgaatcactaagtgagagccaatcaatctcagagagcgaatca
ctaagtgaaagtcaatcaatttcagagagtgaatcactaagcgagagccaatcaatctca
gagagtgaatcattaagtgaaagtcagtcaatttcagagagtgaatcactaagtgaaagt
cagtcaatttcagaaagcgaatcattaagtgaaagccaatcaatcagtgaaagcgaatca
ctaagcgagagtcaatcaatctcagagagcgaatcattaagtgaaagtcaatcaatttca
gaaagcgagtcattaagcgagagtcagtcaatctcagagagcgaatcactaagcgagagt
caatcaatctcagagagtgaatcattaagtgagagccaatcagttagtgaaagcgaatca
ctaagtgaaagtcagtcaatttcagaaagcgaatcattaagtgagagtcaatcaatttca
gaaagcgaatcattaagtgaaagccaatcaatcagtgaaagcgaatcactaagcgagagc
caatcaatcagtgaaagcgaatcattaagtgagagtcaatcaatctcagaaagcgaatca
ttaagtgagagtcaatcaatcagtgaaagcgaatcactaagcgagagccaatcaatctca
gagagcgaatcactaagcgagagccaatcaatctcagagagcgagtcactaagcgagagc
caatcaatcagtgaaagcgaatcattaagtgagagtcaatcaatcagtgaaagcgagtca
ctaagtgagagccaatcaatctcagagagtgaatcattgagtgagagccaatcaatctca
gagagcgagtcactaagtgagagtcaatcaatttcagagagcgaatcattaagtgaaagc
caatcaatctcagagagtgaatcattgagtgagagccaatcagttagtgaaagcgagtca
ctaagtgagagtcaatcaatcagtgaaagcgagtcactaagtgagagtcaatcaatttca
gagagcgaatcattaagcgagagtcagtcaatctcagagagtgaatcactaagtgagagc
caatcaatctcagagagtgaatcattaagtgagagccaatcaatctcagagagtgaatca
ctaagtgagagtcaatcaatcagtgaaagcgaatcactaagcgagagccaatcaatttca
gagagtgaatcattaagtgagagccaatcagttagtgaaagcgaatcactaagcgagagc
caatcaatctcagagagcgaatcattgagtgagagccaatcaatctcagagagtgaatca
ttgagtgagagtcaatcaatcagtgaaagcgaatcactaagcgaaagtcaatcaatttca
gagagtgaatcattgagtgagagccaatcaatttcagagagtgaatcactaagtgaaagt
cagtcaatttcagaaagcgaatcactaagcgagagccaatcaatctcagagagcgaatca
ctaagtgaaagtcagtcaatttcagaaagcgaatcattaagtgaaagccaatcaatctca
gagagtgaatcattaagtgaaagtcagtcaatttcagagagtgaatcactaagtgaaagt
cagtcaatttcagaaagcgaatcattaagcgagagtcagtcaatttcagaaagcgaatca
ttaagtgaaagccaatcaatcagtgaaagcgaatcactaagcgagagccaatcaatctca
gagagcgaatcactaagcgagagccaatcaatctcagagagcgaatcactaagtgaaagt
caatcaatttcagagagtgaatcattgagtgagagtcaatcaatttcagagagtgaatca
ctaagtgaaagtcaatcaatttcagagagtgaatcactaagcgagagccaatcaatctca
gagagtgaatcattaagtgaaagtcagtcaatttcagagagggaatcactaagtgaaagt
cagtcaatttcagaaagcgaatcattaagtgaaagccaatcaatcagtgaaagcgaatca
ctaagtgaaagtcaatcaatctcagagagtgaatcactaagtgagagccaatcaatctca
gagagtgaatcattgagtgagagccaatcaatctcagagagcgaatcactaagtgaaagt
caatcaatttcagaaagcgagtcattaagcgagagtcagtcaatctcagagagtgaatca
ctaagtgagagccaatcaatctcagagagtgaatcactaagtgagagtcaatcaatcagt
gaaagcgaatcactaagcgagagccaatcaatttcagagagtgaatcattaagtgagagc
caatcagttagtgaaagcgaatcactaagcgagagccaatcaatctcagagagcgagtca
ctaagcgagagtcaatcaatctcagagagtgaatcactaagtgaaagtcagtcaatttca
gaaagcgagtcactaagcgagagtcaatcaatctcagagagtgaatcattgagtgagagc
caatcaatctcagagagcgaatcattgagtgagagccaatcaatctcagagagtgaatca
ttgagtgagagccaatcaatttcagagagcgaatcactaagcgagagccaatcaatcagt
gaaagcgaatcattaagtgagagtcagtcaattagcgaaagcgaatcactaagtgagagt
caatcaatctcagagagtgaatcactaagtgaaagtcagtcaatcagcgaaagcgaatct
aaatctttacctaataccggtactggagaaaagatttctaattatccaggtattttagga
ggattattaagcatattaggtataagtttgcttaaaagaaaagacagagagaaaaaatta
ggacaaaaatctaataagtag
The protein sequence translated from SEQ ID NO 31 is designated SEQ
ID NO: 32 and is shown below:
TABLE-US-00016 SEQ ID NO: 32 MKSKYDFLPN RLNKFSIRKF TVGSVSVLIG
ATLLFGFVEG EASASVKEGQ QSINSSEKES ADPTVVDLIS KKETNLDGLD VSREETTKVP
INENKRGEEQ SISDKAITEK ADTPVSNLSS KEVEEQGVSD KAITEKADTP VTNLSSKEAK
EQGASDRVIT EKADTPVSNL SSKEAKEQGA SDRVITEKAD TPVSNLSSKE VEEQGVSDKA
IEKIADASAT DLSSKEEVEQ DISTQGKVKS KEAVQVESSQ LQNLNSEINA EPNEIKAIDR
SSILPLNLND EENNKKVNKG TRVPEATLRN ASNNQLNTRM RSVSLFRVAR LTEINRNVND
KVKVSDIDIA IAPPHTNPKT GKEEFWATSS SVLKLKASYE LDNSISKGDQ FTIQFGQNIR
PGGLNLPRPY NFLYDKDKKL VATGRYNKES NTITYTFTDY VDKHQNIKGS FEMNAFSRKE
NATTDKTAYP MDVTIANQKY SENIIVDYGN KKNAAIISST EYIDLDGSRK MTTYINQNGS
KNSIYRADMQ IDLNGYKFDP SKNNFKIYEV ENSSDFVDSF SPDVSKLRDV TSQFNIQYTN
NNTMAKVDFG TNLWRGKKYI IQQVANIDDS KLVKNASINY TLNKMDFNNK RTVETHNNTY
STVKDKSTAL GDVQESQSIS ESQSVSESES LSESQSISES ESLSESQSIS ESESLSESQS
ISESESLSES QSISESESLS ESQSISESES LSESQSISES ESLSESQSIS ESESLSESQS
ISESESLSES QSISESESLS ESQSISESES LSESQSISES ESLSESQSIS ESESLSESQS
ISESESLSES QSISESESLS ESQSISESES LSESQSISES ESLSESQSIS ESESLSESQS
ISESESLSES QSISESESLS ESQSISESES LSESQSISES ESLSESQSIS ESESLSESQS
ISESESLSES QSISESESLS ESQSISESES LSESQSISES ESLSESQSIS ESESLSESQS
ISESESLSES QSISESESLS ESQSVSESES LSESQSISES ESLSESQSIS ESESLSESQS
ISESESLSES QSISESESLS ESQSISESES LSESQSISES ESLSESQSIS ESESLSESQS
ISESESLSES QSISESESLS ESQSISESES LSESQSISES ESLSESQSIS ESESLSESQS
ISESESLSES QSISESESLS ESQSVSESES LSESQSISES ESLSESQSIS ESESLSESQS
ISESESLSES QSISESESLS ESQSISESES LSESQSISES ESLSESQSIS ESESLSESQS
VSESESLSES QSISESESLS ESQSISESES LSESQSISES ESLSESQSIS ESESLSESQS
ISESESLSES QSISESESLS ESQSISESES LSESQSISES ESLSESQSIS ESESLSESQS
ISESESLSES QSISESESLS ESQSISESES LSESQSISES ESLSESQSIS ESESLSESQS
ISESESLSES QSISESESLS ESQSISESES LSESQSISES ESLSESQSIS ESESLSESQS
ISERESLSES QSISESESLS ESQSISESES LSESQSISES ESLSESQSIS ESESLSESQS
ISESESLSES QSISESESLS ESQSISESES LSESQSISES ESLSESQSIS ESESLSESQS
ISESESLSES QSVSESESLS ESQSISESES LSESQSISES ESLSESQSIS ESESLSESQS
ISESESLSES QSISESESLS ESQSISESES LSESQSISES ESLSESQSIS ESESLSESQS
ISESESLSES QSISESESLS ESQSISESES KSLPNTGTGE KISNYPGILG GLLSILGISL
LKRKDREKKL GQKSNK SEQ ID NO: 33
atgttaagaacaaattataaactaagaaagcttaaagtaggtttagtatcgacaggtgtg
gcgttgacttttgtgatggcaagtgggaatgcagaggcgtcggagaacgagcagactgaa
gtaaaaggggaggcgcaagttgcttctgtgaatgaaaaagagagtgaagcagaattacct
gtagcgcaacaagaagcatctattcaactagacaaagtacaaccaggcgatgcacagctt
tcaggctatacacagccaaacaaagcgatttctgtaaagatcgacaataaagatattgtg
tctgtagatgatggctatgaagaggtattatcggatgatacaggtaaatttgtatatgat
ttgaaagggcgtcaaattgtttacaatcaaaaagttgatgttgaagcgatgacgccattt
aattttgaagattttgatgaatcagcacttgagagcgaagaggcattggaggcgttaggt
caattggaagacgaagaaacagcgacagcttctgtgacgacgcctagatatgaaggtgcg
tatacagttcctgaagaacgcttgacacccattcaaggccaacagcaagtattcatcgaa
cctattttagaaggggcaagtaaaatcaaaggacatacatctgtacaaggtaaagtcgcg
ttagcaatcaatcaagaacatgtgcacctaggtgatacgttagaagaacaagcagcactc
actgatcaagagtggcaaggtcgttatgacgggatttggcgccatattgatgatcaaggg
tttttcgagtttgacttgaaccgtctttacaataaatcttacccattgaagtctggcgat
ttagtgactttatcttttaaatctaatgacgaagtaggcccattattcaatgtgaacgtt
gagcctttcgaacgtgtggcacaagctaaaacaaagtatgagcagaatgacagtccagta
gtcaacaaattggatgatactaaaagtgacttggaggttcaacctatctatggagacctt
acacaagcagcagtacatggcgagtcgaaagtgttgataccggggacgtcaaaagttgaa
ggacgtacgaattatgcacatgcatggatagagatggcatctaatttaggggaatatcgt
agtttccctaaattacaagctgatgcgacaggtgcgtttatatttgatttaaaagcggca
gacatacaattgttaaacggagaacgtttgacattcagagccgttgacccacatacaaaa
caacagttagctgaaactacatcagaagtacgcccagtagatatgcaagatgaagagtca
gaggttgtgcagacttcaagcactgagaaatcagcacttgcggatgaaattcttcgttct
atgacaattgacaaatcatttaatcctgaagttaccgagataccgggtcatgtatatcct
aagaaaacagaggataaaggtgctgaaaatacagaacaagcctcagagaattctgagaag
ccatctcagactacagaatctcaaaatgatgccgtacaagatgtagagaaatcctctgtt
aatgaggaggttacgccaccttcaacagaatctgctcaagttgaaaaggggcaaaataca
gaaggggctttgcttccaaaaaatgtagaacaacatgtagagagtataccataccaaaaa
cgtaaagcgttgataggactgacaaaacatcaaggatcagggcacatgccgccattttct
ttaagctttaataataaagaagatgacgtatccacaaaggttaacgaagcaaacgagcat
gaacgtaagcagggtacagtttatccagagcaaatagaacaattacctcaaacaggttta
actgaaaaatcgccattctgggcattgttatttgttgtatcaggcacaggtttattatta
ttcaaacgttctagacgacaacgccaatcttaa
The protein sequence translated from SEQ ID NO 33 is designated SEQ
ID NO: 34 and is shown below:
TABLE-US-00017 SEQ ID NO: 34
MLRTNYKLRKLKVGLVSTGVALTFVMASGNAEASENEQTEVKGEAQVASV
NEKESEAELPVAQQEASIQLDKVQPGDAQLSGYTQPNKAISVKIDNKDIV
SVDDGYEEVLSDDTGKFVYDLKGRQIVYNQKVDVEAMTPFNFEDFDESAL
ESEEALEALGQLEDEETATASVTTPRYEGAYTVPEERLTPIQGQQQVFIE
PILEGASKIKGHTSVQGKVALAINQEHVHLGDTLEEQAALTDQEWQGRYD
GIWRHIDDQGFFEFDLNRLYNKSYPLKSGDLVTLSFKSNDEVGPLFNVNV
EPFERVAQAKTKYEQNDSPVVNKLDDTKSDLEVQPIYGDLTQAAVHGESK
VLIPGTSKVEGRTNYAHAWIEMASNLGEYRSFPKLQADATGAFIFDLKAA
DIQLLNGERLTFRAVDPHTKQQLAETTSEVRPVDMQDEESEVVQTSSTEK
SALADEILRSMTIDKSFNPEVTEIPGHVYPKKTEDKGAENTEQASENSEK
PSQTTESQNDAVQDVEKSSVNEEVTPPSTESAQVEKGQNTEGALLPKNVE
QHVESIPYQKRKALIGLTKHQGSGHMPPFSLSFNNKEDDVSTKVNEANEH
ERKQGTVYPEQIEQLPQTGLTEKSPFWALLFVVSGTGLLLFKRSRRQRQS SEQ ID NO: 35
atgaaaactaaatacacagcaaaattattaattggggcagcaacaatatctttagcaaca
tttatttcacaagggaacgcacatgcgagcgaacaaactacaggactcgcaccggcacaa
cctgtcaactttgattcaatcaatgtaacgccagaccaaaaaacattctatcaagtctta
catatggaaggcatttcagaagaccaacgtgaacaatatttgaaacaattgcacgaagac
ccaagtagcgcacaaaatgttttttcagaatcaattaaagatgccatccacccggaacgt
cgtgttgcgcaacaaaatgcgttttacagcgtattacacaacgatgacttatccgaagag
caacgtgatgcatacattggtagaattaaagaagatccagatcaaagccaagaagtattt
gttgagtctttaaatgtggcacctaaagcagaatcacatgaagatcgcctcattgaatta
caaaacaaaaatttaatggaagcgaatgaagcacttaaagcgttacaacaagaagacagc
attcagaatagacgtgcggctcaacgtgctgtcaacaaattgacgccggatagcgcgaac
gcattccaaaaagaattagatcaaatcaatgccccacgcgacgctaaaattaaagctgac
gctgaagcaaaaaaacaagcacctgaagtaagcgcaccacaaattgaagatgcacctact
actgaagttgcaccatctccaaaacaagatatgccaaaagtagataaaaaagaagaagat
aaagtagaaagtgatactgaggtcaaagaagtacctaaagctgatacagagaaaaaccct
caatctaaagacacttctaaaactgaacaagctaaagaaacacctaaagtagagcaatca
cctaaaacagaaaaggctgaagaagcacctaaagcagaaacacctcaaaatggaaataaa
gcacaaactgaagaagctaaaccagaagtaaaagacaatgtgaaaaacactccatctgca
cctgtgttacctgaaacaggaaaagcaacaacttcaacacttgaaagctactggaattct
ttcaaagacagtgtgaataaaggttatacttacattaaacaaagcttagaaagtggttat
caatatttaaaaggtcaatacgactatatcactaaaaaatacaatgatgcgaaatactat
acaaaaatgtattcaaatcataagtctacaattgatcagtctgtattagctatattaggt
aaaactggatctagcgcatatatcaagccattaaatatcgaagaaaattcaaacgtattt
tacaaagcttatgcaaaaacaagaaactttgctacagaaagcattaacacaggaaaagta
ttatacacattatatcaaaaccctactgtagttaaatctgctttcactgcaattgaaaca
gcaaatacagtaaaaaatgcaataagcaatcttttctctctcttcaaataa
The protein sequence translated from SEQ ID NO 35 is designated SEQ
ID NO: 36 and is shown below:
TABLE-US-00018 SEQ ID NO: 36
MKTKYTAKLLIGAATISLATFISQGNAHASEQTTGLAPAQPVNFDSINVT
PDQKTFYQVLHMEGISEDQREQYLKQLHEDPSSAQNVFSESIKDAIHPER
RVAQQNAFYSVLHNDDLSEEQRDAYIGRIKEDPDQSQEVFVESLNVAPKA
ESHEDRLIELQNKNLMEANEALKALQQEDSIQNRRAAQRAVNKLTPDSAN
AFQKELDQINAPRDAKIKADAEAKKQAPEVSAPQIEDAPTTEVAPSPKQD
MPKVDKKEEDKVESDTEVKEVPKADTEKNPQSKDTSKTEQAKETPKVEQS
PKTEKAEEAPKAETPQNGNKAQTEEAKPEVKDNVKNTPSAPVLPETGKAT
TSTLESYWNSFKDSVNKGYTYIKQSLESGYQYLKGQYDYITKKYNDAKYY
TKMYSNHKSTIDQSVLAILGKTGSSAYIKPLNIEENSNVFYKAYAKTRNF
ATESINTGKVLYTLYQNPTVVKSAFTAIETANTVKNAISNLFSLFK
An active domain from the protein of SEQ ID NO: 6 is designated SEQ
ID NO: 37
TABLE-US-00019 SEQ ID NO: 37
NEDVTETTGRNSVTTQASEQHLQVEAVPQEGNNVNVSSVKVPTNTATQAQ
EDVASVSDVKAHADDALQVQESSHTDGVSSEFKQETAYANPQTAETVKPN
SEAVHQSEYEDKQKPVSSSRKEDETMLQQQQVEAKNVVSAEEVSKEENTQ
VMQSPQDVEQHVGGKDISNEVVVDRSDIKGFNSETTIRPHQGQGGRLNYQ
LKFPSNVKPGDQFTIKLSDNINTHGVSVERTAPRIMAKNTEGATDVIAEG
LVLEDGKTIVYTFKDYVNGKQNLTAELSVSYFVSPEKVLTTGTQTFTTMI
GNHSTQSNIDVYYDNSHYVDGRISQVNKKEAKFQQIAYINPNGYLNGRGT
IAVNGEVVSGTTKDLMQPTVRVYQYKGQGVPPESITIDPNMWEEISINDT
MVRKYDGGYSLNLDTSKNQKYAIYYEGAYDAQADTLLYRTYIQSLNSYYP
FSYQKMNGVKFYENSASGSGELKPKPPEQPKPEPEIQADVVDIIEDSHVI DIGW
An spsl gene fragment corresponding to A domain is designated SEQ
ID NO: 38, which encodes the protein of SEQ ID NO: 37
TABLE-US-00020 SEQ ID NO: 38
AATGAAGATGTCACTGAAACAACTGGGAGAAATTCAGTGACAACGCAAGC
TTCTGAGCAACATTTGCAAGTGGAAGCAGTACCTCAAGAAGGCAATAATG
TAAATGTATCCTCTGTAAAAGTACCTACGAATACGGCAACGCAAGCACAA
GAAGATGTTGCAAGTGTATCCGATGTTAAAGCACATGCTGATGATGCATT
ACAAGTACAAGAAAGTAGTCATACTGATGGTGTTTCTTCAGAATTCAAGC
AGGAGACAGCTTATGCGAATCCTCAAACAGCTGAGACAGTTAAACCTAAT
AGTGAAGCAGTGCATCAGTCTGAATACGAGGATAAGCAAAAACCCGTATC
ATCTAGCCGCAAAGAAGATGAGACTATGCTTCAGCAGCAACAAGTTGAAG
CCAAAAATGTTGTGAGTGCGGAGGAAGTGTCTAAAGAAGAAAATACTCAA
GTGATGCAATCCCCTCAAGACGTTGAACAACATGTAGGTGGTAAAGATAT
CTCTAATGAGGTTGTAGTGGATAGGAGTGATATCAAAGGATTTAACAGCG
AAACTACTATTCGACCTCATCAGGGACAAGGTGGTAGGTTGAATTATCAA
TTAAAGTTTCCTAGCAATGTAAAGCCAGGCGATCAGTTTACTATAAAATT
ATCTGACAATATCAATACACATGGTGTTTCTGTTGAAAGAACCGCACCGA
GAATCATGGCTAAAAATACTGAAGGTGCGACGGATGTAATTGCTGAAGGT
CTAGTGTTGGAAGATGGTAAAACCATCGTATATACATTTAAAGACTATGT
AAATGGCAAGCAAAATTTGACTGCTGAGTTATCAGTGAGCTATTTCGTAA
GTCCGGAAAAAGTCTTGACTACTGGGACACAAACATTCACGACGATGATC
GGTAATCATTCAACGCAATCCAATATTGACGTTTATTATGATAATAGTCA
TTATGTAGATGGACGTATTTCGCAAGTGAACAAAAAAGAAGCTAAATTTC
AACAAATAGCATACATTAACCCTAATGGCTATTTAAATGGCAGGGGGACA
ATTGCAGTTAATGGTGAAGTGGTCAGTGGTACGACTAAAGACTTAATGCA
ACCTACAGTGCGTGTATATCAATATAAAGGACAAGGTGTTCCTCCTGAAA
GTATTACTATAGACCCTAATATGTGGGAAGAAATCAGCATAAACGATACT
ATGGTAAGAAAATATGATGGTGGCTATAGCTTGAATCTGGATACCAGCAA
GAATCAAAAATATGCCATCTATTATGAAGGGGCATATGATGCGCAAGCTG
ACACACTGTTGTATAGAACATATATACAGTCATTAAACAGTTACTATCCG
TTCAGTTACCAAAAAATGAACGGTGTGAAGTTTTACGAAAACAGTGCGAG
TGGAAGCGGTGAGTTGAAACCGAAACCACCTGAACAACCAAAACCAGAAC
CTGAAATTCAAGCTGATGTAGTAGATATTATTGAAGATAGCCATGTGATT GATATAGGATGG
[0009] Since each of the abovementioned proteins/nucleic acid
sequences is derived from Staphylococcus pseudintermedius, the
inventors have designated these (and the corresponding protein
sequences) Staphylococcus pseudintermedius surface genes/nucleic
acids/proteins (Sps). For simplicity, the bulk of this
specification will use the term "Sps" or "Sps genes" or "Sps
nucleic acids" which are intended to encompass all of the nucleic
acid sequences described above (i.e. SEQ ID NOS: 1, 3, 5, 7, 9, 11,
13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33 and 35.
[0010] Furthermore, in addition to encompassing the entire or
complete gene/nucleic sequences listed above, it is to be
understood that the designation "Sps" also encompasses fragments,
portions, mutants, derivatives and/or homologoues/orthologues of
any of these genes.
[0011] In addition, the term "Sps" or "Sps proteins" encompasses
the proteinaceous products of the Sps genes/nucleic acids or
fragments, portions, analogues, variants or derivatives thereof
(for example short peptide fragments). In particular, the term "Sps
proteins" encompasses the sequences given as SEQ ID NOS: 2, 4, 6,
8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36 and 37
above.
[0012] Typically, the gene/nucleic acid fragments, portions,
mutants, variants, derivatives and/or homologues/orthologues of the
invention are functional or active--that is, they retain the
function and/or activity of the wild type or native Sps
genes/nucleic acids. Advantageously, fragments, portions, mutants,
variants, derivatives and/or homologues/orthologues of any of the
Sps genes/nucleic acids provided by this invention, encode proteins
(or peptides, peptide fragments) retaining the ability to bind to
or associate with extracellular matrix proteins such as, for
example, fibrinogen, fibronectin and/or collagen. In other
embodiments, the proteins and/or peptides encoded by the nucleic
acid sequences described herein are immunogenic or antigenic.
Furthermore, fragments, portions, variants or derivatives of any of
the proteins encoded by the nucleic acid sequences described herein
may also retain the immunogenicity and/or antigenicity of a
corresponding wild type Sps protein (for example the proteins
listed above). Where the invention relates to immunogenic
compositions and/or vaccines, the use of proteins and/or peptides
which are immunogenic (or antigenic) is important.
[0013] The term "mutants" may encompass naturally occurring mutants
or those artificially created by the introduction of one or more
nucleic acid additions, deletions, substitutions or inversions.
[0014] Homologous or identical genes, nucleic acid or protein
sequences may exhibit as little as approximately 20 or 30% sequence
homology or identity to certain reference sequences, however, in
other cases, homologous or identical genes/nucleic acids and/or
proteins may exhibit at least 40, 50, 60, 65, 70, 75, 80, 85, 90,
91, 92, 93, 94, 95, 96, 97, 98, 99% homology or identity to the
various sequences given above as SEQ ID NOS: 1-36 or 37-38. It
should be understood that mutant, variant, derivative and/or
orthologuous sequences may exhibit similar levels of
homology/identity to each other and/or to the Sps genes/nucleic
acids shown as SEQ ID NOS 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21,
23, 25, 27, 29, 31, 33, 35 and/or 38 above.
[0015] One of skill in this field will readily understand that
genes/nucleic acids homologous/identical to the Sps genes detailed
herein may be found in other bacterial species. As such, homologous
genes from other species may be included within the scope of this
invention. Using the various nucleic acid and amino acid sequences
described herein, one of skill in the art could readily identify
related sequences in other microbial (particularly bacterial)
species. For example, nucleic acid obtained from a particular
bacterial species may be probed using the probes derived from the
sequences of this invention, to identify homologous or closely
related sequences.
[0016] It should be understood that Sps nucleic acid sequences of
this invention may be single-stranded or double-stranded and a
single-stranded nucleic acid molecule may include a polynucleotide
fragment having a nucleotide sequence that is complementary to a
nucleotide sequence that encodes a Sps protein or fragment thereof.
As used herein, the term "complementary" refers to the ability of
two single stranded polynucleotide fragments to base pair with each
other.
[0017] A single-stranded nucleic acid molecule of the invention may
further include a polynucleotide fragment having a nucleotide
sequence that is substantially complementary to a nucleotide
sequence that encodes a Sps protein or fragment thereof according
to the invention, or to the complement of the nucleotide sequence
that encodes said Sps protein or fragment thereof. Substantially
complementary polynucleotide fragments can include at least one
base pair mismatch, such that at least one nucleotide present on a
first polynucleotide fragment will not base pair to at least one
nucleotide present on a second polynucleotide fragment, however the
two polynucleotide fragments will still have the capacity to
hybridize. The present invention therefore encompasses
polynucleotide fragments which are substantially complementary. Two
polynucleotide fragments are substantially complementary if they
hybridize under hybridization conditions exemplified by 2.times.SSC
(SSC: 150 mM NaCl, 15 mM trisodium citrate, pH 7.6) at 55.degree.
C. Substantially complementary polynucleotide fragments for
purposes of the present invention may preferably share at least
about 60, 65, 70, 75, 80 or 85% nucleotide identity, preferably at
least about 90%, 95% or 99% nucleotide identity. Locations and
levels of nucleotide sequence identity between two nucleotide
sequences can be readily determined using, for example, CLUSTALW
multiple sequence alignment software.
[0018] In addition, it should be understood that the present
invention also relates to the products of the genes/nucleic acids
encompassed by this invention and in particular to proteins or
peptides homologous/identical to those having sequences provided by
SEQ ID NOS: 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30,
32, 34, 36 and 37. Furthermore, fragments, portions, analogues,
variants, derivatives of any of these or homologous and/or
identical or modified proteins are also within the scope of this
invention. Typically, fragments, portions, derivatives, variants
and/or homologous or modified proteins or peptides of the invention
are functional or active--that is they retain the function of a
wild type Sps protein. In certain embodiments fragments, portions,
derivatives or variants of, and/or modified sequences or sequences
with homology or identity to, the amino acid sequences provided by
this invention, retain the ability to bind to or associate with
extracellular matrix proteins such as, for example, fibrinogen,
fibronectin and/or collagen.
[0019] Additionally or alternatively, fragments, portions, mutants,
variants, derivatives and/or homologues/orthologues of the Sps
genes provided by this invention, may encode proteins (or peptide
fragments) that are antigenically similar or identical to the
proteins encoded by the genes described herein. Similarly,
fragments, portions, derivatives and/or variants of and/or modified
sequences or sequences with homology or identity to, the amino acid
sequences provided by this invention are also antigenically similar
or identical to the proteins encoded by the genes described herein.
It should be understood that the term "antigenically similar or
identical" may encompass proteins or peptides eliciting an immune
response similar or identical to the immune response elicited by
any of the Sps proteins described herein. In certain embodiments
fragments, portions, derivatives and/or variants of and/or modified
sequences or sequences with homology or identity to, the amino acid
sequences described herein, elicit immune responses which protect
against Staphylococcus pseudintermedius infection and/or prevent,
reduce or neutralise Staphylococcus pseudintermedius cell/tissue
adhesion and/or colonisation. One of skill will readily understand
that the antigenicity of a polypeptide can be evaluated in vitro
by, for example, performing a Western blot on the purified
polypeptide (for example, an affinity purified polypeptide) using
polyclonal antisera from an animal, such as a rabbit that was
vaccinated with at least an antigenic portion of an Sps protein of
the present invention.
[0020] One of skill in this field will readily understand that for
the various nucleic acid sequences and polypeptides described
herein, natural variations due to, for example, polymorphisms, may
exist between Sps genes and proteins isolated from different
microbial species and even different strains of the same species.
Gene or protein variants may manifest as proteins and/or genes that
exhibit one or more amino acid/nucleic acid substitutions,
additions, deletions and/or inversions relative to a reference
sequence (for example any of the sequences described above). As
such, it is to be understood that all such natural variants,
especially those that are functional or display the desired
activity, are to be included within the scope of this
invention.
[0021] In another embodiment, the invention relates to derivatives
of any of the Sps sequences described herein. The term
"derivatives" may encompass Sps genes or peptide sequences which,
relative to those described herein, comprise one or more amino acid
substitutions, deletions, additions and/or inversions.
[0022] Additionally, or alternatively, analogues of the various
peptides described herein may be produced by introducing one or
more conservative amino acid substitutions into the primary
sequence. One of skill in this field will understand that the term
"conservative substitution" is intended to embrace the act of
replacing one or more amino acids of a protein or peptide with an
alternate amino acid with similar properties and which does not
substantially alter the physcio-chemical properties and/or
structure or function of the native (or wild type) protein.
Analogues of this type are also encompassed with the scope of this
invention. In one embodiment, substitute amino acids may be
selected from other members of the class to which the amino acid
belongs. For example, nonpolar (hydrophobic) amino acids include
alanine, leucine, isoleucine, valine, praline, phenylalanine,
tryptophan, and tyrosine. Polar neutral amino acids include
glycine, serine, threonine, cysteine, tyrosine, asparagine and
glutamine. The positively charged (basic) amino acids include
arginine, lysine and histidine. The negatively charged (acidic)
amino acids include aspartic acid and glutamic acid. Examples of
preferred conservative substitutions include Lys for Arg and vice
versa to maintain a positive charge; Glu for Asp and vice versa to
maintain a negative charge; Ser for Thr so that a free --OH is
maintained; and Gln for Asn to maintain a free NH.sub.2.
[0023] As is well known in the art, the degeneracy of the genetic
code permits substitution of one or more bases in a codon without
changing the primary amino acid sequence. Consequently, although
the sequences described in this application are known to encode the
Sps proteins described herein, the degeneracy of the code may be
exploited to yield variant nucleic acid sequences which encode the
same primary amino acid sequences.
[0024] The present invention may further provide modified Sps
proteins. For example, a "modified" Sps protein may be chemically
and/or enzymatically derivatised at one or more constituent amino
acids, including side chain modifications, backbone modifications,
and N- and C-terminal modifications including acetylation,
hydroxylation, methylation, amidation, phosphorylation and the
attachment of carbohydrate or lipid moieties, cofactors, and the
like.
[0025] One of skill in this field will appreciate that the amino
acid and/or nucleic acid sequences described herein may be used to
generate recombinant Sps genes/proteins and as such, the present
invention further contemplates methods of generating and/or
expressing recombinant Sps genes and/or proteins, and products for
use in such methods. Accordingly, in addition to providing
substantially purified or isolated recombinant Sps sequences, a
second aspect of this invention provides DNA constructs comprising
a replicable expression vector and nucleic acid encoding one or
more of the Sps protein(s) described herein.
[0026] Expression vectors for the production of the molecules of
the invention include plasmids, phagemids, viruses, bacteriophages,
integratable DNA fragments, and other vehicles, which enable the
integration of DNA fragments into the genome of the host.
Expression vectors are typically self-replicating DNA or RNA
constructs containing the desired gene or its fragments, and
operably linked genetic control elements that are recognised in a
suitable host cell to effect expression of the desired genes.
[0027] Generally, the genetic control elements can include a
prokaryotic promoter system or a eukaryotic promoter expression
control system. Such systems typically include a transcriptional
promoter, an optional operator to control the onset of
transcription, transcription enhancers to elevate the level of RNA
expression, a sequence that encodes a suitable ribosome binding
site, RNA splice junctions, sequences that terminate transcription
and translation and so forth. Expression vectors usually contain an
origin of replication that allows the vector to replicate
independently of the host cell.
[0028] A vector may additionally include appropriate restriction
sites, antibiotic resistance or other markers for selection of
vector containing cells.
[0029] Plasmids are the most commonly used form of vector but other
forms of vectors which serve an equivalent function and which are,
or become, known in the art are suitable for use herein. See, e.g.,
Pouwels et al. Cloning Vectors: a Laboratory Manual (1985 and
supplements), Elsevier, N.Y.; and Rodriquez, et al. (ads.) Vectors:
a Survey of Molecular Cloning Vectors and their Uses, Buttersworth,
Boston, Mass. (1988).
[0030] In general, such vectors may contain specific genes, which
are capable of providing phenotypic selection in transformed cells.
The use of prokaryotic and eukaryotic viral expression vectors to
express the nucleic acid sequences coding for the recombinant
proteins of the present invention are also contemplated.
[0031] The vector is introduced into a host cell by methods known
to those of skill in the art. Introduction of the vector into the
host cell can be accomplished by any method that introduces the
construct into the cell, including, for example, electroporation,
heat shock, chemical compounds such, for example, calcium
phosphate, stronitium phosphate, microinjection techniques and/or
gene guns. See, e.g., Current Protocols in Molecular Biology,
Ausuble, F. M., ea., John Wiley & Sons, N.Y. (1989).
[0032] Another aspect relates to a host cell transformed with any
one of the nucleic acid constructs of the present invention.
Suitable host cells include prokaryote cells, lower eukaryotic and
higher eukaryotic cells. Prokaryotes include Gram negative and Gram
positive organisms, e.g., E. coli and B. subtilis. Lower eukaryotes
include yeast, S. cerevisiae and Pichia, and species of the genus
Diclyostelium.
[0033] "Host cell" as used herein refers to cell which can be
recombinantly transformed with vectors constructed using
recombinant DNA techniques.
[0034] A drug resistance or other selectable marker is intended in
part to facilitate the selection of the transformants.
Additionally, the presence of a selectable marker, such as a drug
resistance marker may be of use in keeping contaminating
microorganisms from multiplying in the culture medium. Such a pure
culture of the transformed host cells would be obtained by
culturing the cells under conditions which require the induced
phenotype for survival.
[0035] PCR techniques may be exploited to selectively obtain Sps
gene sequences from samples of Staphylococcal DNA. These amplified
sequences may be introduced into any of the vectors described
above. In one embodiment, the vector may further comprise a
nucleotide sequence of a tag or label to assist in protein
purification procedures.
[0036] Techniques used to purify recombinant proteins generated in
this way are known and, where the recombinant protein is tagged or
labelled, these may include the use of, for example, affinity
chromatography techniques.
[0037] In view of the above, a fourth aspect of this invention
provides a process for the production of recombinant Sps protein(s)
or peptide(s) of the invention, said process comprising the steps
of (a) transforming a host cell with the nucleotide sequence of the
invention or transfecting a host cell with a nucleic acid construct
of the invention; (b) culturing the cells obtained in (a) under
conditions in which expression of the protein takes place; and (c)
isolating the expressed recombinant protein or peptide from the
cell culture or/and the culture supernatant.
[0038] The polypeptide may be partially purified from the host and
where the polypeptide is secreted from the host cell, the cells may
be separated from the media by centrifugation, the cells being
pelleted. Alternatively, the polypeptide may be partially purified
from this supernatant, for example using affinity
chromatography.
[0039] A fifth aspect of this invention provides monoclonal or
polyclonal antibodies, whether derived from rodents, mammals,
avians, ungulates, or other organisms, that bind to the Sps
proteins described herein. Production and isolation of monoclonal
and polyclonal antibodies to a selected polypeptide sequence is
routine in the art see for example "Basic methods in Antibody
production and characterisation" Howard & Bethell, 2000, Taylor
& Francis Ltd. Such antibodies may be used in diagnostic
procedures, as well as for passive immunisation.
[0040] Staphylococcus pseudintermedius is known to cause cutaneous
inflammatory diseases in a variety of animals. One such cutaneous
inflammatory disease is canine pyoderma which is a major cause or
morbidity in dogs. Pydoderma associated with Staphylococcus
pseudintermedius infection is common among dogs and is often
associated with puritis, alopecia, erythema and swelling. At
present, the treatment of this infection is difficult, requiring
the use of aggressive, systemically administered antibiotics. The
present inventors have discovered that Sps genes (and their protein
products) play a role in Staphylococcus pseudintermedius
colonisation and pathogenesis. As such, the Sps genes and proteins
described herein may find application in the treatment and/or
prevention of cutaneous disorders such as canine pyoderma.
[0041] Accordingly, a sixth aspect of this invention provides an
Sps protein or gene as substantially defined above, for use in
raising an immune response in an organism. The proteins and genes
described herein may find particular application as a vaccine, but
could also be used to obtain an immune serum potentially useful in
passive vaccination techniques.
[0042] Advantageously, the invention may provide a vaccine for use
in preventing or controlling disease in canine species caused or
contributed to by Staphylococcus pseudintermedius. In other
embodiments, the vaccines provided by this invention may be used to
protect against the development of infections caused or contributed
to by Staphylococcus pseudintermedius. In other embodiments, the
vaccines may be used to protect against instances of canine
pyoderma.
[0043] In one embodiment, the vaccine may be a polypeptide and/or
polynucleotide vaccine.
[0044] A polynucleotide vaccine may comprise a polynucleotide
fragment, preferably a DNA fragment, having a nucleotide sequence
encoding an antigenic polypeptide comprising at least an antigenic
portion any one or more of the Sps proteins described herein.
Vaccines of this type may otherwise be referred to as "DNA
vaccines"--such vaccines may be introduced to host cells (such as
mammalian, for example, canine cells) where they express antigens
which elicit immune responses.
[0045] A polypeptide or protein vaccine may comprises one or more
of the Sps proteins (or antigentic fragments or portions) described
herein. One of skill will appreciate that the one or more Sps
protein(s) may be naturally occurring and isolated from
Staphylococcus pseudintermedius, or recombinant.
[0046] A protein vaccine may be administered by any suitable route.
Advantageously, a protein vaccine may be administered orally (by
ingestion), topically or by direct injection--preferably
intraperitoneal or intramuscular injection. A protein subunit
vaccine formulated for oral administration can contain the
polypeptide encapsulated in for example, a biodegradable polymer as
described hereinafter.
[0047] In view of the above, the invention further provides a
method of immunising a dog against Staphylococcus pseudintermedius,
said method comprising administering to the dog a DNA or protein
vaccine of the invention.
[0048] Conveniently, the protein vaccines described herein may
further include or comprise one or more adjuvant(s). Further, one
or more booster vaccinations are preferably administered at time
periods subsequent to the initial administration to create a higher
level of immune response in the animal.
[0049] In yet another aspect, the vaccine of the invention may
comprise a fusion protein comprising a carrier polypeptide and one
or more Sps protein(s) of the invention. The Sps protein(s) for use
in this aspect of the invention can itself be antigenic or
non-antigenic; in embodiments wherein the protein is non-antigenic,
the carrier polypeptide is antigenic, stimulating the immune system
to react to the fusion protein thereby generating an immune
response in an organism--such as, for example a canine immune
response to Staphylococcus pseudintermedius. A non-antigenic
protein thus functions as a hapten. An example of an antigenic
carrier polypeptide is keyhole limpet hemocyanim (KLH).
Conventional fusion constructs between carriers such as glutathione
sulfotransferase (GST) and said Sps protein(s) of the invention are
also included as protein vaccines according to the invention, as
are fusions of the Sps protein(s) and an affinity tag such as a
polyhistidine sequence. A fusion construct may be preferred for use
as a protein vaccine when the antigenic Sps analog, fragment, or
modification thereof is small.
[0050] In a seventh aspect, the present invention provides a method
for immunising dogs against Staphylococcus pseudintermedius, said
method comprising administering to the dog a vaccine of the
invention.
[0051] A polynucleotide vaccine may further comprises a promoter,
such as the CMV promoter, operably linked to the coding sequence
for the Sps polypeptide or antigenic fragment thereof (e.g., U.S.
Pat. No. 5,780,44, Davis). The polynucleotide may be cloned within
a vector such as a plasmid. There are numerous plasmids known to
those of ordinary skill in the art useful for the production of
polynucleotide vaccines.
[0052] Other possible additions to the polynucleotide vaccine
constructs include nucleotide sequences encoding cytokines, such as
granulocyte macrophage colony stimulating factor (GM-CSF),
interleukin-12 (IL-12) and co-stimulatory molecules such B7-1,
B7-2, CD40. The cytokines can be used in various combinations to
fine-tune the response of the animal's immune system, including
both antibody and cytotoxic T lymphocyte responses, to bring out
the specific level of response needed to affect the animal's
reproductive system. A polynucleotide vaccine of the invention can
also encode a fusion product containing the antigenic polypeptide
and a molecule, such as CTLA-4, that directs the fusion product to
antigen-presenting cells inside the host.
[0053] Plasmid DNA can also be delivered using attenuated bacteria
as delivery system, a method that is suitable for DNA vaccines that
are administered orally. Bacteria are transformed with an
independently replicating plasmid, which becomes released into the
host cell cytoplasm following the death of the attenuated bacterium
in the host cell. An alternative approach to delivering the
polynucleotide to an animal involves the use of a viral or
bacterial vector. Examples of suitable viral vectors include
adenovirus, polio virus, pox viruses such as vaccinia, canary pox,
and fowl pox, herpes viruses, including catfish herpes virus,
adenovirus-associated vector, retroviruses and bacteriophage.
Exemplary bacterial vectors include attenuated forms of Salmonella,
Shigella, Edwardsiella ictaluri, and Yersinia ruckeri. Preferably,
the polynucleotide is a vector, such as a plasmid, that is capable
of autologous expression of the nucleotide sequence encoding said
Sps protein or fragment thereof.
[0054] In one embodiment, the vaccine may be a DNA vaccine
comprising a DNA fragment having a nucleotide sequence that encodes
a polypeptide having an amino acid sequence homologous or identic
to a sequence selected from the group consisting of SEQ ID NOS: 2,
4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36 or
37 or an antigenic analog, fragment, or modified version
thereof.
[0055] Polynucleotide-based immunisation induces an immune response
to an antigen expressed in vivo from a heterologous polynucleotide
fragment introduced into a cell. DNA vaccine may be particularly
useful as the heterologous nucleic acid expression may continue for
a length of time sufficient to induce a relatively strong and
sustained immune response without the need for subsequent "booster"
vaccinations, as may be required when using protein based vaccines.
A polynucleotide vaccine comprising a polynucleotide fragment
having a nucleotide sequence encoding said Sps can be administered
to dog (or rather to a particular tissue or cells thereof) using
biolistic bombardment, ingestion or direct injection, as described
for example, in U.S. Pat. No. 5,780,448 (Davis), preferably
intraperitoneal or intramuscular injection. A preferred method of
administration is biolistic bombardment, as with a "gene gun". A
polynucleotide vaccine formulated for oral administration
preferably contains DNA encapsulated in a biodegradable polymer.
Examples of a suitable biodegradable polymer include chitosan and
homo- or co-polyers of polylactic acid and polyglycolic acid.
Accordingly, the present invention further provides a method for
immunising dogs against Staphylococcus pseudintermedius by
administering to the dog a polynucleotide vaccine of the invention,
preferably a DNA vaccine.
[0056] Other methods of administering nucleic acid vaccines of the
type described herein may include, for example, use of the
technology described in WO02/076498.
[0057] The amount of protein/polynucleotide vaccine to be
administered to an animal depends on the type and size of animal,
the condition being treated, and the nature of the
protein/polynucleotide, and can be readily determined by one of
skill in the art. In some applications, one or more booster
administrations of the protein/polynucleotide vaccine at time
periods subsequent to the initial administration are useful to
create a higher level of immune response in the animal.
[0058] In one embodiment of the vaccine of the invention and/or Sps
proteins described herein (including antigenic fragments, analogs
or modified version thereof) may be linked, for example, at its
carboxy-terminus, to a further component. The further component may
serve to facilitate uptake of the Sps protein, or enhance its
immunogenicity/processing.
[0059] The immune-stimulating compositions of the invention may be
optionally mixed with excipients or diluents that are
pharmaceutically acceptable as carriers and compatible with the
active component(s). The term "pharmaceutically acceptable carrier"
refers to a carrier(s) that is "acceptable" in the sense of being
compatible with the other ingredients of a composition and not
deleterious to the recipient thereof. Suitable excipients are well
known to the person skilled in the art. Examples include; water,
saline (e.g. 0.85% sodium chloride; see Ph.Eur. monograph
2001:0062), buffered saline, fish oil with an emulsifier (e.g. a
lecithin, Bolec MT), inactivant (e.g. formaldehyde; see Ph.Eur.
monograph 1997:0193), mineral oils, such as light mineral oils,
alhydrogel, aluminium hydroxide. Where used herein, the term "oil
adjuvant" to embraces both mineral oils and synthetic oils. A
preferred adjuvant is Montanide ISA 711 (SeppicQuai D'Orsay, 75321
Paris, France) which is a manide oleate in an oil suspension. In
addition, if desired, the immune-stimulating composition (including
vaccine) may contain minor amounts of auxiliary substances such as
wetting or emulsifying agents, pH buffering agents, and/or
adjuvants which enhance the effectiveness of the immune-stimulating
composition.
[0060] A vaccine composition may be administered as a course of a
number of discrete doses over a period of time. For example it may
be administered over a period of around 2-21 days.
[0061] Vaccination may be repeated at daily, twice-weekly, weekly
or monthly intervals. For example a boost vaccination may be
administered after the initial dose. For example a boost may be
administered at around 4-14 weeks after the vaccination. The
initial vaccination and any boost may be carried out using the same
or different modes of administration. For example, the initial may
be by injection and the boost may be by oral administration. An
example regime includes a first vaccination by injection, followed
by a course of orally administered boost vaccine, or a booster
prior to an expected outbreak. However, it will be appreciated that
any suitable route of administration(s) and/or regime(s) may be
employed.
[0062] Additionally, knowledge of the Sps protein nucleotide and
amino acid sequences set forth herein opens up new possibilities
for detecting, diagnosing and characterising Staphylococcus
pseudintermedius in canine populations. For example, an
oligonucleotide probe or primer based on a conserved region of one
or more of the Sps proteins described herein, may be used to detect
the presence of the Sps protein in or on a canine host.
[0063] Vaccines may contain one or more of the Sps proteins/nucleic
acids/genes described herein (i.e. those shown as SEQ ID NOS:
1-38). In one embodiment, the vaccine may comprise a cocktail of
Sps proteins/peptides and or nucleic acids. Typically, a cocktail
may comprise 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16,
17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33,
34, 35, 36, 37 or 38 Sps nucleic acid and/or protein/peptide
components (for example (2 or more) components having sequences
homologous or identical to any of SEQ ID NOS: 1-38).
[0064] Furthermore, the vaccines may contain bacterial antigens
used to control other diseases, for example diseases caused by
other Staphylococcal species and/or antigens to treat, prevent or
control diseases and/or conditions with other aetiologies or caused
or contributed to by other pathogens. As such, the vaccine
compositions described herein may find application in multivalent
vaccines including antigens against other canine diseases.
[0065] In addition to vaccines and/or immunogenic compositions
comprising one or more of the Sps proteins described herein, the
present invention further provides compounds for treating
infections caused or contributed to by Staphylococcus
pseudintermedius or compounds for the preparation of medicaments
for treating the same.
[0066] In one embodiment, the compound may be a small organic
molecule, antibody, peptide or carbohydrate which antagonises the
interaction between the Sps protein and its ligand (an
extracellular matrix (ECM) protein). For example, the compound may
be a synthetic peptide comprising or based on, the sequence of an
ECM protein known to interact with a particular Sps protein, or the
sequence of a protein given above which may interfere with binding
between the wild type S. psedintermedius protein and its ligand.
Additionally or alternatively, binding agents, such as for example,
antibodies with specificity or affinity for one or more Sps protein
ligands, may also be used to antagonise the Sps/ligand interaction.
Therapeutic approaches of this type may prevent Staphylococcus
pseudintermedius colonising or binding/adhering to cells.
[0067] In view of the above, the invention may relate to methods of
treating infections caused or contributed to by Staphylococcus
pseudintermedius, said method comprising administering to an animal
a therapeutically effective amount of a compound which antagonises
Sps/ligand interactions.
[0068] In a further aspect, the present invention provides
pharmaceutical compositions comprising a compound which antagonises
Sps/ligand interactions together with a pharmaceutical excipient,
carrier or diluent.
[0069] One of skill will appreciate that the vaccines, methods,
uses or medicaments comprising any of the Sps genes/nucleic acids
and/or proteins and/or antagonistic compounds (for example Sps
protein/nucleic acid fragments and/or antibodies) described herein
may be combined with one or more other compounds for treating one
or more other conditions--in particular one or more other skin
conditions. Said other skin condition may be, for example, atopic
dermatitis.
[0070] In a further aspect, the present invention provides methods
of diagnosing infections, diseases and/or conditions caused or
contributed to by S. pseudintermedius, said methods comprising the
steps of identifying in a sample provided by a subject suspected of
suffering from an infection, disease and/or conditions S.
pseudintermedius caused or contributed to by S. pseudintermedius, a
level of a protein, peptide or nucleic acid (for example a gene)
encoded by a sequence provided by SEQ ID NOS: 1-38 or a fragment,
portion, mutant, derivative and/or homologoue/orthologue
thereof.
[0071] It should be understood that all methods of diagnosis or
detection described herein, may include an optional step in which
the results are compared with the results of a control sample,
which does not comprise sequences derived from S. pseudintermedius,
in particular sequences corresponding to those provided as SEQ ID
NOS: 1-38 disclosed herein.
[0072] The term "sample" may be taken to mean any sample comprising
protein and/or nucleic acid. For example, a "sample" may comprise a
bodily fluids such as whole blood, plasma, serum, saliva, sweat
and/or semen. In other instances "samples" such as tissue biopsies
and/or scrapings may be used. In particular, cutaneous (i.e. skin)
tissue biopsies and/or scrapings may be used. Advantageously such
biopsies may comprise cells obtained from lesions suspected of
resulting from or being associated with a S. pseudintermedius.
Specifically, a biopsy, tissue sample or scraping may comprise
cells derived from lesions exhibiting pathology characteristic of
the S. pseudintermedius disease, pyoderma (particularly caninine
pyoderma).
[0073] In addition, a sample may comprise a tissue or gland
secretion and washing protocols may be used to obtain samples of
fluid secreted into or onto various tissues, including, for
example, the skin. One of skill in this field will appreciate that
the samples described above may yield or comprise quantities of
nucleic acid (i.e. DNA or RNA) encoding all or part of the various
proteins described herein as well as quantities of proteins or
peptides (or fragments thereof) encoded thereby. In one embodiment,
the sample may comprise quantities of nucleic acid/peptide having
or comprising the sequences given as SEQ ID NOS: 1-38.
[0074] One of skill in the art will be familiar with the techniques
that may be used to identify levels of certain nucleic acid
sequences and/or proteins, such as, for example, levels of the
sequences given as SEQ ID NOS: 1-38 described herein (or a
fragment, portion, mutant, derivative and/or homologoue/orthologue
thereof).
[0075] For example, PCR based techniques may be used to detect
levels of gene expression or gene quantity in a sample. Useful
techniques may include, for example, polymerase chain reaction
(PCR) or reverse transcriptase (RT)-PCR based techniques in
combination with real-time PCR (otherwise known as quantitative
PCR).
[0076] Additionally, or alternatively, a level of gene/protein
expression may be identified by way of microarray analysis. Such a
method would involve the use of a DNA micro-array which comprises
nucleic acid derived from one or more of the nucleic acid sequences
described herein (for example SEQ ID NOS: 1, 3, 5, 7, 9, 11, 13,
15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 38). To identify a
level of gene expression, one of skill in the art may extract
nucleic acid, preferably mRNA, from a sample and subject it to an
amplification protocol such as, for example RT-PCR to generate
cDNA. Preferably, primers specific for a certain mRNA sequence--in
this case a S. pseudintermedius sequence comprised with any of, for
example, SEQ ID NOS: 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25,
27, 29, 31, 33, 35, 38.
[0077] The amplified cDNA may be subjected to a further
amplification step, optionally in the presence of labelled
nucleotides (as described above). Thereafter, the optionally
labelled amplified cDNA may be contacted with the microarray under
conditions which permit binding with the DNA of the microarray. In
this way, it may be possible to identify a level of S.
pseudintermedius gene expression.
[0078] Further information regarding the PCR based techniques
described herein may be found in, for example, PCR Primer: A
Laboratory Manual, Second Edition Edited by Carl W. Dieffenbach
& Gabriela S. Dveksler: Cold Spring Harbour Laboratory Press
and Molecular Cloning: A Laboratory Manual by Joseph Sambrook &
David Russell: Cold Spring Harbour Laboratory Press.
[0079] In addition, other techniques such as deep sequencing and/or
pyrosequencing may be used to detect cSCC sequences in any of the
samples described above. Further information on these techniques
may be found in "Applications of next-generation sequencing
technologies in functional genomics", Olena Morozovaa and Marco A.
Marra, Genomics Volume 92, Issue 5, November 2008, Pages 255-264
and "Pyrosequencing sheds light on DNA sequencing", Ronaghi, Genome
Research, Vol. 11, 2001, pages 3-11.
[0080] In addition to the molecular detection methods described
above, one of skill will also appreciate that immunological
detection techniques such as, for example, enzyme-linked
immunosorbent assays (ELISAs) may be used to identify levels of S.
pseudintermedius proteins in samples. In other embodiments,
ELISPOT, dot blot and/or Western blot techniques may also be used.
In this way, samples provided by subjects suffering from S.
pseudintermedius related diseases and/or infections (for example
canine subjects suffereing from canine pyoderma), may be probed for
levels of one or more S. pseudintermedius proteins, particularly
those encoded by SEQ ID NOS: 2, 4, 6, 8, 10, 12, 14, 16, 18, 20,
22, 24, 26, 28, 30, 32, 34, 36, or 37 so as to detect the presence
of such proteins in a sample which may indicate a S.
pseudintermedius infection.
[0081] Immunological detection techniques, may require the use of a
substrate to which an antibody and/or antigen may be bound,
conjugated or otherwise immobilised.
[0082] Suitable substrates may comprise, for example, glass,
nitrocellulose, paper, agarose and/or plastic. A substrate which
comprises, for example, a plastic material, may take the form of a
microtitre plate.
[0083] Further information regarding ELISA procedures and protocols
relating to the other immunological techniques described herein may
be found in Using Antibodies: A Laboratory Manual by Harlow &
Lane (CSHLP: 1999) and Antibodies: A Laboratory Manual by Harlow
& Lane (CSHLP: 1988).
[0084] The present invention also extends to kits comprising
reagents and compositions suitable for diagnosing, detecting or
evaluating possible S. pseudintermedius infections, diseases and/or
conditions. Kits according to this invention may be used to
identify and/or detect levels of S. pseudintermedius gene(s)/S.
pseudintermedius protein(s) in samples. Depending on whether or not
the kits are intended to be used to identify levels of S.
pseudintermedius genes and/or S. pseudintermedius proteins in
samples, the kits may comprise substrates having S.
pseudintermedius proteins or agents capable of binding S.
pseudintermedius proteins, bound thereto. In addition, the kits may
comprise agents capable of binding S. pseudintermedius
proteins--particularly where the kit is to be used to identify
levels of one or more S. pseudintermedius proteins in samples. In
other embodiments, the kit may comprise polyclonal antibodies or
monoclonal antibodies which exhibit specificity and/or selectivity
for one or more S. pseudintermedius proteins. Antibodies for
inclusion in the kits provided by this invention may be conjugated
to detectable moieties. Kits for use in detecting the expression of
genes encoding S. pseudintermedius proteins may comprise one or
more oligonucleotides/primers for detecting/amplifying/probing
samples for S. pseudintermedius protein encoding sequences. The
kits may also comprise other reagents to facilitate, for example,
sequencing, PCR and/or RFLP analysis. In one embodiment, the kits
may comprise one or more oligonucleotides/primers for
detecting/amplifying/probing nucleic acid samples (for example
nucleic acid derived from canine skin) for levels of sequences
corresponding to all or part of those described as SEQ ID NOS: 1-38
herein.
BRIEF DESCRIPTION OF THE DRAWINGS
[0085] FIG. 1: Genomic location of the 17 genes encoding putative
CWA proteins in S. pseudintermedius strain ED99.
[0086] FIG. 2: Distribution of the genes encoding putative CWA
proteins among 20 S. pseudintermedius strains, representatives of
the closely related S. delphini and S. intermedius, and other
staphylococcal species associated with animal skin disease.
[0087] FIG. 3: SDS-PAGE analysis (A) and Western blot analysis (B)
of cell wall-associated proteins of S. Pseudintermedius ED99 and L.
lactis expressing SpsD, SpsL, and SpsO with sera from dogs
diagnosed with pyoderma.
[0088] FIG. 4. Adherence of L. lactis expressing specified MSCRAMMs
to human Fn.
[0089] FIG. 5. Adherence of L. lactis expressing specified MSCRAMMs
to Fg from different animal sources.
[0090] FIG. 6. Adherence of L. lactis expressing specified MSCRAMMs
to CK10.
[0091] FIG. 7. Adherence of L. lactis expressing different MSCRAMMs
to canine corneocytes of five dogs.
[0092] FIG. 8: SDS-PAGE analysis (A) and Western blot analysis (B)
of Sps D, Sps L and Sps O recombinant A domain with canine
convalescent serum from pyoderma cases.
[0093] FIG. 9: Inhibition of adherence of L. lactis expressing SpsD
(A) and SpsL (B), and S. aureus Newman (C) to fibrinogen by canine
convalescent serum from pyoderma cases.
DETAILED DESCRIPTION
[0094] The invention will now be described in more detail with
reference to the following Figures which show:
[0095] FIG. 1. Genomic location of the 17 genes encoding putative
CWA proteins in S. pseudintermedius strain ED99. Eight genes are
situated in the oriC environ, indicated in orange, and nine are
located in the core genome. sps=S. pseudintermedius surface
protein.
[0096] FIG. 2. Distribution of the genes encoding putative CWA
proteins among 20 S. pseudintermedius strains, representatives of
the closely related S. delphini and S. intermedius, and other
staphylococcal species associated with animal skin disease. The
diversity of strains is represented a phylogenetic tree; grey
squares indicate that the gene is present, blank squares that the
gene is absent based on Southern blot analysis (for spsA to spsO),
or PCR amplification (for spsP and spsQ).
[0097] FIG. 3. Western blot analysis of cell wall-associated
proteins of S. pseudintermedius ED99 and L. lactis expressing SpsD,
SpsL, and SpsO with sera from dogs diagnosed with pyoderma. (A) SDS
PAGE analysis and (B) Western blot analysis of protein fractions
from S. pseudintermedius ED99 in exponential phase of growth (lane
1); L. lactis expressing SpsL (lane 2); L. lactis expressing SpsD
(lane 3); L. lactis expressing SpsO (lane 4); and L. lactis with
pOri23 alone (lane 5).
[0098] FIG. 4. Adherence of L. lactis expressing specified MSCRAMMs
to human Fn. Plates were coated with 1 .mu.g of human Fn or 1 .mu.g
of BSA per well. Absorbance was measured at 590 nm and results are
expressed as mean values of triplicate samples. Error bars indicate
standard deviation. L. lactis expressing FnbpA from S. aureus and
PBS were included as controls.
[0099] FIG. 5. Adherence of L. lactis expressing specified MSCRAMMs
to Fg from different animal sources. Plates were coated with 1
.mu.g of Fg or 1 .mu.g of BSA per well. Absorbance was measured at
590 nm and results are expressed as mean values of triplicate
samples. Error bars indicate standard deviation. L. lactis
expressing FnbpA from S. aureus and PBS were included as
controls.
[0100] FIG. 6. Adherence of L. lactis expressing specified MSCRAMMs
to CK10. Plates were coated with 1 .mu.g of recombinant CK10 or 1
.mu.g of BSA per well. Absorbance was measured at 590 nm and
results are expressed as mean values of triplicate samples. Error
bars indicate standard deviation. S. aureus strain SH1000 in
exponential and stationary phases of growth and PBS were included
as controls.
[0101] FIG. 7. Adherence of L. lactis expressing different MSCRAMMs
to canine corneocytes of five dogs. Bacterial adherence is
calculated as percentage area covered with bacterial cells per
field of corneocytes (ROI=500 .mu.m.sup.2). Results are based on
the arithmetic mean of duplicate experiments. The bottom of each
box represents the first quartile (Q1), the top of the box the
third quartile (Q3), the bold lines the median, and the black
circles the mean values. The whiskers define the range of the
data.
[0102] FIG. 8: Reactivity of canine convalescent serum from
pyoderma cases to Sps D, Sps L and Sps O recombinant A domain. 1 ug
aliquots of rSps D and rSps L, and 10 .mu.l volumes of purified
rSps O were subjected to SDS-PAGE under standard conditions and
Coomassie stained (A) or Western blot transferred onto a
nitrocellulose membrane. Membranes were probed with a 1:1000
dilution of canine serum, followed by a 1:3000 dilution of
HRP-conjugated sheep anti-canine IgG. Reactive bands were
visualized on Chemi-luminescent Film (B). 5 .mu.l aliquots of
recombinant ClfB and the superantigen SEI from S. aureus were
included in the terminal lanes of each gel as negative
controls.
[0103] FIG. 9: Inhibition of adherence of L. lactis expressing SpsD
(A) and SpsL (B) to fibrinogen (2 .mu.g per well) by canine
convalescent serum from pyoderma cases. Bacterial cultures,
normalised to an OD600 of 1 in PBS were pre-incubated for 2 h with
doubling dilutions of serum ranging from 2% to .about.0.01% (v/v),
prior to inoculation into fibrinogen coated wells. Results (n=3,
.+-.SD) are expressed as absorbance readings at 590 nm minus
background levels of fluorescence. Background fluorescence was
measured by inoculating control cultures, incubated for 2 h in the
absence of serum, into wells coated with BSA (2 .mu.g per well).
Incubations of S. aureus Newman (C) were included as a negative
control.
EXAMPLES
Example 1
Materials and Methods
Genome-Wide Screen for Genes Encoding for Cell-Wall Anchored
Proteins
[0104] The S. pseudintermedius strain ED99 draft genome was
interrogated for homologous sequences using position specific
iterative basic local alignment search tool (PSI-BLAST), available
from the National Center for Biotechnology Information (NCBI), USA
(http://blast.ncbi.nlm.nih.gov/Blast.cgi), and for the presence of
a LPX[TSA][GANS] motif pattern by pattern hit initiated basic local
alignment search tool (PHI-BLAST), available from NCBI
(http://blast.ncbi.nlm.nih.gov/Blast.cgi). Signal sequences were
predicted by employing the SignalP server
(http://www.cbs.dtu.dk/services/SignalP/), provided by the Center
for Biological Sequence Analysis (CBS), Technical University of
Denmark.
In Silico Structural Analysis of Cell-Wall Anchored Proteins
[0105] The predicted CWA proteins were searched for functional
domains using EMBL-EBI InterPro Scan
(http://www.ebi.ac.uk/interpro). Structural analysis was carried
out with the PHYRE (Protein Homology/analogY Recognition Engine)
fold recognition server, available from the Structural
Bioinformatics Group, Imperial College London, UK
(http://www.sbg.bio.ic.ac.uk/phyre/). Repeat sequences were
predicted by generating nucleic acid dot plots, using software
available from Colorado State University, USA
(http://www.vivo.colostate.edu/molkit/dnadot/), applying tandem
repeats finder software from Boston University, USA
(http://tandem.bu.edu/trf/trf.html), and variable sequence tandem
repeats extraction and architecture modelling (XSTREAM), available
from the University of California, USA
(http://jimcooperlab.mcdb.ucsb.edu/xstream/). Sequence alignments
and pair-wise sequence comparisons were generated with ClustalW2
(http://www.ebi.ac.uk/Tools/clustalw2). Amino acid composition and
molecular weight predictions were generated using ProtParam on the
ExPASy Proteomics Server
(http://www.expasy.ch/tools/protparam.html).
Cloning of Selected Genes Encoding Putative MSCRAMMs of S.
pseudintermedius ED99 into L. lactis MG1363
[0106] Oligonucleotides were designed for PCR amplification of the
full-length spsD, spsL and spsO genes and either PstI, SalI or
BamHI specific restriction sites were inserted on both sides of the
DNA fragments. 50 .mu.l PCR reactions contained 2 .mu.l
(approximately 100 ng) genomic DNA template, 0.25 .mu.M forward
primer, 0.25 .mu.M reverse primer, 1.times.PfuUltra.TM. II reaction
buffer (Stratagene, USA), 0.25 mM dNTP's (Promega, USA) and 1 .mu.l
PfuUltra.TM. II Fusion HS DNA polymerase (Stratagene, USA). The
thermocycler programme included an initial denaturation step at
95.degree. C. for 2 min, followed by 30 cycles of denaturation at
95.degree. C. for 20 s, annealing at 50.degree. C. for 20 s and
extension at 72.degree. C. for 2 min, followed by a final extension
step at 72.degree. C. for 3 min. PCR products were visualised on
0.8% (w/v) agarose gels, gel extracted under avoidance of UV light
exposure and purified using QIAquick Gel Extraction Kit (Qiagen,
UK) according to the manufacturer's instructions. Purified DNA
fragments were cloned into the StrataClone.TM. Blunt PCR cloning
vector pSC-B (Stratagene, USA) using the StrataClone Ultra.TM.
Blunt PCR Cloning Kit (Stratagene, USA) according to the
manufacturer's instructions. Each cloning reaction consisted of 3
.mu.l Strataclone Buffer Blunt (Stratagene, USA), 2 .mu.l purified
PCR product and 1 .mu.l Strataclone.TM. Blunt Vector Mix
(Stratagene, USA). StrataClone.TM. SoloPack.RTM. competent cells
(Stratagene, USA) were transformed according to the manufacturer's
instructions and colonies selected using blue-white screening on
LB-ampicillin (100 .mu.g/ml)-X-gal plates. White colonies were
transferred into 5 ml LB-ampicillin (100 m/ml) broth and grown
overnight at 37.degree. C. with shaking at 200 rpm. Plasmid was
isolated using QIAprep Spin Miniprep Kit (Qiagen, UK) according to
the manufacturer's instructions. Purified plasmids were digested
using appropriate restriction endonucleases (New England Biolabs,
UK), and diagnostic digests were analysed on 0.8% (w/v) agarose
gels. For generating DNA constructs, the E. coli-L. lactis shuttle
vector pOri23 (kindly provided by P. Moreillon, University of
Lausanne, Switzerland) was used. The pOri23 vector carries the
ermAM gene for erythromycin resistance, the high-copy-number
oriColE1 replicon for autonomous replication in E. coli and the
constitutive lactococcal promoter P23 (Que et al., 2000). The
multiple cloning site of pOri23 consists of restriction sites for
endonucleases PstI, SalI and BamHI (Que et al., 2000).
[0107] StrataClone.TM. plasmids containing the DNA inserts of
interest and the E. coli-L. lactis shuttle vector pOri23 were each
digested in a 100 .mu.l total reaction volume containing 10 .mu.l
plasmid (approximately 2.5 .mu.g), 20 units appropriate restriction
endonucleases (New England Biolabs, UK), and suitable buffers (New
England Biolabs, UK) according to the manufacturer's instructions.
Restriction digestions were performed at 37.degree. C. for 16 h.
The restriction fragments to be cloned were extracted from 0.8%
(w/v) agarose gels without UV exposure as described in the general
Material and Methods and purified using QIAquick Gel Extraction Kit
(Qiagen, UK) according to the manufacturer's instructions. DNA
inserts and restriction-digested pOri23 plasmid were quantified
using spectrophotometry (NanoDrop ND-1000, Thermo Scientific, USA)
and ligation reactions were carried out with a plasmid to insert
ratio of 1:3 in a 10 .mu.l total ligation reaction volume,
consisting of 1 .mu.l vector (approximately 10 ng), 400 units T4
DNA ligase (New England Biolabs, UK), lx T4 DNA ligase reaction
buffer (New England Biolabs, UK), x ill DNA insert (depending on
DNA concentration), and x .mu.l sterile water (depending on the
volume of DNA insert). Ligations were incubated at 16.degree. C.
for 16 h.
[0108] One 50 .mu.l aliquot of electrocompetent L. lactis cells was
thawed on ice and 2 .mu.l (.about.20 ng) pOri23 plasmid carrying
the DNA insert of interest was added. Electroporation cuvettes
(Sigma-Aldrich, UK) were pre-chilled and L. lactis cells plus
plasmid were transferred into the cuvettes. Electroporation was
performed at standard settings (25 .mu.F, 2.5 kV, 200 Ohm) and 1 ml
GM17 was added immediately. Cells were incubated at 30.degree. C.
in a static incubator for 2 h prior to spreading 250 .mu.l of cell
suspension per plate onto GM17 plates containing 5 .mu.g/ml
erythromycin. Plates were incubated overnight at 30.degree. C.
[0109] For screening of L. lactis transformants, plasmid was
isolated using the Qiagen MiniPrep Kit (Qiagen, UK) with addition
of 100 U/ml mutanolysin (Sigma-Aldrich, UK) and 100 m/ml lysozyme
(Sigma-Aldrich, UK) to buffer P1. Diagnostic digests of purified
plasmids were carried out with appropriate restriction enzymes and
analysed on 0.8% (w/v) agarose gels.
[0110] Additionally, colony PCR was performed for pOri23 carrying
spsD and spsO using gene-specific oligonucleotides (Table 5.3). L.
lactis colonies were resuspended in 10 .mu.l 10% (v/v) IGEPAL
(Sigma-Aldrich, UK) and incubated for 10 min at 95.degree. C. in a
thermocycler machine. 40 .mu.l master mix containing 0.3 .mu.M
forward primer, 0.3 .mu.M reverse primer, 0.2 mM dNTP's (Promega,
USA), 1.times. reaction buffer (Promega, USA), 1.5 mM MgCl.sub.2
(Promega, USA) and 0.025 u/.mu.l taq polymerase (Promega, USA) was
added. The thermocycler programme included an initial denaturation
step at 95.degree. C. for 2 min, followed by 30 cycles of
denaturation at 95.degree. C. for 1 min, annealing at 50.degree. C.
for 1 min and extension at 72.degree. C. for 1 min, followed by a
final extension step at 72.degree. C. for 7 min. PCR products were
visualised on 0.8% (w/v) agarose gels.
Western Blot Analysis of L. lactis Constructs
[0111] Samples were dissolved in 1.times. Laemmli sample buffer
(Sigma-Aldrich, UK), boiled for 10 min and resolved by SDS-PAGE in
10% polyacrylamide gels by standard procedures, and Western blot
analysis was carried out as described in the general Materials and
Methods. Three canine sera samples from pyoderma cases (obtained
from patients at the Hospital for Small Animals, The Royal (Dick)
School of Veterinary Studies, The University of Edinburgh) were
pooled and used as primary antibody in a 1:1000 dilution.
HRP-conjugated sheep anti-dog antibody was used as a secondary
antibody in a 1:5000 dilution (Bethyl Laboratories Inc., USA).
Canine Corneocyte Adherence Assay
[0112] For preliminary experiments to confirm adherence of S.
pseudintermedius ED99 and non-adherence of L. lactis, corneocytes
were obtained from a seven-year-old male neutered Border collie
cross-breed with no history or physical signs of systemic or
cutaneous disease. Corneocytes for the L. lactis adherence study
were obtained from five dogs of different breeds (one Labrador
retriever, two Border collies and two cross-breeds). Three dogs
were ovariohysterectomised females and two were entire males. The
median age was seven years (range one to twelve years). The dogs
showed no abnormalities on general physical examination and had no
history or physical signs of skin disease at the time of corneocyte
collection. All dogs were privately owned by staff or students of
the Royal (Dick) School of Veterinary Studies, The University of
Edinburgh. None of the dogs had received topical or systemic drug
treatments for at least three weeks prior to the day of corneocyte
collection.
[0113] Samples were taken from the ventral abdomen and inner thigh.
If necessary, sample sites were clipped with Oster clippers (Oster
Cryotech, USA) using a number 40 blade. For collection of
corneocytes, the method described by Forsythe et al. (2002) was
used. Briefly, the area was cleaned of surface debris and commensal
bacteria by applying four strips of single sided adhesive tape
(Cellux, Henkel Consumer Adhesives, UK), using each strip once. To
collect corneocytes, double-sided, clear, adhesive wig tape
(Tropical Tape Super Grip, USA) was mounted onto a microscope slide
in 1 cm.sup.2 pieces and applied to the same area of skin surface
10 times with gentle force. Slides were investigated by microscopic
examination and only slides with at least 75% corneocyte coverage
were used in the study.
[0114] The corneocyte slides were positioned in moisture chambers
(Nunc.TM., Thermo Fisher Scientific, Denmark) as described by
Forsythe et al. (2002). The moisture chambers consisted of 30
cm.times.30 cm plastic trays with lids and were prepared by lining
the trays with moistened paper towels. S. pseudintermedius ED99
stationary or exponential (OD.sub.600 of 0.5) phase cultures and L.
lactis exponential phase cultures (OD.sub.600 0.6 to 0.8) were
centrifuged at 4000 rpm for 5 min, washed with PBS and resuspended
in PBS to a final OD.sub.600 of 0.5. The moisture chambers were
placed in a static incubator and 250 .mu.l of bacterial suspension
was added to each 1 cm.sup.2 of tape, forming a meniscus on the
tape. Slides incubated with 250 .mu.l of sterile PBS were included
as a control. The slides were incubated at 37.degree. C. for 90 min
and washed in PBS. Each slide was stained with 0.5% (w/v) crystal
violet (Sigma-Aldrich, UK) for 90 s before rinsing off with PBS.
The slides were air-dried and a drop of immersion oil (Cargille
Laboratories Inc., USA) and a cover slip (Scientific Laboratory
Supplies, UK) were added before microscopic quantification. All
slides were prepared in duplicate on the same day and incubated at
the same time. Prior to incubation with bacterial suspensions or
PBS, each slide was labelled with a letter code to allow
identification after the microscopic analysis. The identification
code on each slide was hidden by a third party for subsequent image
acquisition so that the investigator was blinded to the origin of
the slide. For quantification of adherent bacteria, computerised
image analysis was used as described previously by Forsythe et al.
(2002) with minor modifications. For each slide, bright field
images of 1000.times. oil-immersion fields were acquired with a
Sony DXC-390P 3CCD colour video camera (Scion Corporation, USA)
connected to a Leica Laborlux S microscope (Leica Microsystems UK
Ltd., UK). The RGB video signal from the camera was digitised using
Scion Image (Scion Corporation, USA) installed in a G4 Macintosh
computer (Apple Computer, USA) fitted with a CG-7 frame grabber
(Scion Corporation, USA). For image acquisition, fields equivalent
to 14.4 mm.sup.2 were selected randomly by starting in the bottom
left corner of each slide and moving through the slide in a defined
way using the scale on the microscope stage. A field was discarded
if the corneocyte layer was not confluent, the bacteria were poorly
stained against the background or the field could not be focused
properly. The software used for quantification of bacterial
adherence was set to calculate the percentage area that was covered
by bacteria per confluent layer of corneocytes in a defined region
of interest (ROI) of 500 .mu.m.sup.2 within each image field
acquired. Previous studies by Forsythe et al. (2002) using the same
technique and software have demonstrated that 15 replicates of each
duplicate slide resulted in acceptable coefficients of variation of
approximately 10%. In this study, 25 replicates of each slide were
acquired and the overall mean percentage area of adherence was
determined by calculating the mean of all replicates.
Results
Identification of Genes Encoding 17 Putative Cell-Wall-Anchored
Proteins in the S. Pseudintermedius ED99 Genome
[0115] The initial search for putative CWA proteins identified 34
sequences that fulfilled at least one of the search criteria
(homology to characterised MSCRAMMs in the database, predicted
LPXTG motif or variant near the C terminus, predicted signal
peptide at the N terminus). After gap closure and combination of
incomplete sequences, a total of 17 ORFs encoding putative CWA
proteins with a predicted minimum length of approximately 250 amino
acids was determined. The 17 predicted CWA proteins were designated
`Sps` for Staphylococcus pseudintermedius surface proteins`,
followed by a capital letter (SpsA to SpsQ). Their position in the
S. pseudintermedius ED99 genome is indicated in FIG. 1. Of note,
eight genes encoding putative CWA proteins are located near the
oriC environ (FIG. 1). Homology searches in the database resulted
in sequence identities with known staphylococcal proteins ranging
from .about.30% to .about.80% (Table 1). Signal sequences,
necessary for Sec-dependent protein secretion (Foster and Hook,
1998), were predicted for 14 putative Sps proteins, consisting of
29 aa for SpsC and SpsK, 33 aa for SpsN, SpsP, and SpsQ, 36 aa for
SpsD, 37 for SpsG, 38 aa for SpsA, SpsB, and SpsL, 39 aa for SpsH,
44 aa for SpsO, and 48 aa for SpsF and SpsM. No signal sequence was
predicted for SpsE, SpsI, and SpsJ (FIG. 4.3).
The Putative CWA Proteins SpsD, SpsL, and SpsO have Several MSCRAMM
Features.
[0116] Out of the 17 putative CWA proteins of S. pseudintermedius
ED99, SpsD, SpsL, and SpsO contained each of the MSCRAMM features
screened for, including a signal sequence at the N-terminus,
followed by a non-repeated A domain with two IgG-like folds,
dividing the A domain into N1, N2, and N3 subdomains, a tandemly
repeated domain at the C-terminus (and at the N-terminus for SpsO),
and a C-terminal LPXTG-anchor motif. The main characteristics of
SpsD, SpsL, and SpsO are summarised in Table 2. Of interest, a
TYTFTDYVD motif or variant, important for the `dock, lock and
latch` ligand-binding mechanism (Ponnuraj et al., 2003), was found
in SpsD, SpsL, and SpsO, and putative latching sequences were
identified (Table 2). Further, putative Fn-binding motifs with weak
homology to FnbpA-10 of FnbpA in S. aureus were detected in the
repeat region of SpsL (24% identity in pair-wise alignments for
SpsL1-SpsL6, and 21% for SpsL-7). No homology to Fn-binding motifs
of FnbpA was detected in the repeat regions of SpsD and SpsO. Of
note, the genes encoding for SpsD, SpsL, and SpsO in the S.
pseudintermedius ED99 genome are situated in different genomic
contexts. While spsD is located in a well-conserved region of the
core genome, spsL is part of the oriC environ (Takeuchi et al.,
2005) (FIG. 1). The spsO gene appears to be species-specific as it
is not present in the genomes of other staphylococcal species. The
region contains two putative transposases, suggesting that the
whole region might be subjected to horizontal gene transfer.
Distribution of the 17 Genes Encoding Putative Cell-Wall-Anchored
Proteins Among the S. intermedius Group
[0117] In order to investigate the distribution of the 17 genes
encoding putative CWA proteins identified in the S.
pseudintermedius ED99 genome among other members of the SIG and
closely related staphylococcal species, Southern blot analysis and
PCR amplification were performed. A total of 20 S. pseudintermedius
strains representing the breadth of diversity within the species,
representatives of the closely related S. delphini and S.
intermedius species, and other staphylococcal species associated
with animal hosts (FIG. 2) were screened for the presence of the
putative CWA encoding genes by Southern blot analysis (spsA to
spsO). For the S. aureus spa orthologues spsP and spsQ, PCR
amplification was employed, as the genes share 70% nucleotide
identity which precluded design of gene-specific probes for
Southern blot analysis. For similar reasons, the primers designed
for PCR amplification were located upstream of spsP (spsP-F), in
the non-repeated region of spsP (spsP-R), in the unique region
between spsP and spsQ (spsQ-F), and in a region unique for spsQ
(spsQ-R).
[0118] Of the 17 genes examined, 13 were found in all S.
pseudintermedius strains investigated. The remaining 4 (spsF, spsO,
and the S. aureus spa orthologues spsP and spsQ) were present in
11, 6, 7, and 11 of the 20 S. pseudintermedius strains,
respectively. Furthermore, 8 of the 17 genes were detected in S.
delphini and 6 in S. intermedius, and 9 genes were exclusive to S.
pseudintermedius. None of the genes encoding putative CWA proteins
was detected in the non-SIG staphylococcal species examined.
Results are summarised in FIG. 2. Of note, it cannot be excluded at
this point that DNA sequence variation in PCR primer annealing
sites for spsP and spsQ, and weak homology (less than approximate
70%) for spsA to spsO among different strains have influenced the
results.
Expression of CWA Proteins on the S. pseudintermedius Bacterial
Cell Surface.
[0119] The in silico identification of 17 putative CWA proteins in
S. pseudintermedius ED99 raises questions about the expression of
these proteins and their role in colonisation and disease. Surface
proteome analysis of early-, mid-, and late exponential phase S.
pseudintermedius ED99 was performed in collaboration with the
Moredun Research Institute, Penicuik, Scotland, UK, using liquid
chromatography-electrospray ionisation-tandem mass spectrometry
(LC-ESI-MS-MS). Six out of the 17 putative CWA proteins predicted
in the S. pseudintermedius ED99 genome were detected on the
bacterial surface, including SpsD, SpsK, SpsL, SpsN, SpsO, and
SpsQ. The putative CWA proteins SpsL, SpsN, and SpsQ were
identified in all three phases of growth; SpsK was lacking in
early-, SpsO in mid-, and SpsD in late exponential phase. The 11
undetected CWA proteins might not have been expressed under the
conditions tested, or the expression level might have been below
the detection threshold of the LC-ESI-MS-MS method used.
Cloning and Expression of SpsD, SpsL, and SpsO in L. lactis.
[0120] In order to examine the role of putative selected MSCRAMMs
independently on the bacterial cell surface, the full-length spsD
(3096 bp), spsL (2793 bp) and spsO (5538 bp) genes were cloned into
L. lactis using the shuttle vector pOri23 (Que et al., 2000).
Positive clones were identified by restriction digestion of
purified pOri23 plasmids from single colonies of transformed L.
lactis cells (data not shown). The pOri23 construct inserts were
verified by DNA sequencing for spsL and spsD. For spsO, DNA
sequence was generated for approximately 3000 bp of the total
length of 5538 bp. A segment of the repeat region corresponding to
.about.2500 bp could not be determined due to the existence of
identical tandem repeats which did not allow directed sequencing.
As a negative control for subsequent MSCRAMM characterisation
studies, L. lactis was transformed with the empty vector pOri23,
confirmed by restriction digestion analysis. The predicted
molecular weights were 115 kDa for SpsD, 103 kDa for SpsL, and 198
kDa for SpsO.
[0121] L. lactis Expressing SpsD and SpsL Demonstrated
Seroreactivity with Canine Sera from Pyoderma Cases.
[0122] The potential antibody response to SpsD, SpsL, and SpsO in
vivo was investigated by Western blot analysis employing canine
sera from staphylococcal pyoderma cases. The pyoderma was
clinically manifested at the time of blood sampling and the dogs
were also diagnosed with AD (Neuber et al., 2008). Cell
wall-associated protein fractions of the L. lactis constructs and
of S. pseudintermedius ED99 were subjected to SDS-PAGE, transferred
to nitrocellulose membrane and incubated with pooled canine sera
from three pyoderma cases as described in Materials and Methods. An
array of immunoreactive bands was detected for S. pseudintermedius
ED99, ranging from 24 kDa to 102 kDa in molecular weight (FIG. 3).
For L. lactis expressing SpsD and L. lactis expressing SpsL,
multiple seroreactive bands in the range of 38 kDa to 225 kDa for
SpsD, and 38 kDa and 52 kDa for SpsL were detected, which were
absent in the protein fractions of L. lactis carrying pOri23 alone
(FIG. 3). In contrast, L. lactis expressing SpsO did not
demonstrate seroreactivity with sera from dogs diagnosed with
pyoderma (FIG. 3).
Adherence of L. lactis Constructs to Extracellular Matrix
Proteins.
[0123] L. lactis expressing SpsO, SpsD, SpsL, and L. lactis
carrying the vector pOri23 alone were tested for their ability to
adhere to human Fn, human, canine, feline, and bovine Fg, and to
recombinant mouse CK10 in solid phase assays.
The Putative MSCRAMMs SpsD and SpsL Mediate Binding of L. lactis to
Fibronectin.
[0124] L. lactis expressing SpsD and SpsL demonstrated adherence to
human Fn, whereas L. lactis expressing SpsO demonstrated increased
binding to Fn, but also to BSA, indicative of a non-specific
interaction (FIG. 4).
The Putative MSCRAMMs SpsD and SpsL Mediate Binding of L. lactis to
Fibrinogen, and SpsL Demonstrates Canine Host-Specificity.
[0125] L. lactis expressing SpsD strongly adhered to Fg from
several animal sources (FIG. 5). In contrast, L. lactis expressing
SpsL adhered to canine and feline Fg only, and did not bind to
human and bovine Fg (FIG. 5), indicating a host-specific
interaction. L. lactis expressing SpsO did not bind to Fg from any
source compared to L. lactis with the pOri23 vector alone (FIG.
5).
The Putative MSCRAMM SpsD Mediates Binding of L. lactis to
Cytokeratin 10.
[0126] L. lactis expressing SpsD demonstrated strong adherence to
CK10, whereas L. lactis expressing SpsO and SpsL did not show
increased binding compared to L. lactis with the vector pOri23
alone (FIG. 6).
The Putative MSCRAMMs SpsD and SpsO, but not SpsL, Mediate
Adherence of L. lactis to Ex Vivo Canine Corneocytes.
[0127] L. lactis expressing SpsD, SpsL, and SpsO were tested for
their ability to adhere to ex vivo canine corneocytes in comparison
to L. lactis with the empty vector pOri23 and S. pseudintermedius
ED99. L. lactis carrying the empty vector pOri23 adhered poorly to
canine corneocytes (FIG. 7). For S. pseudintermedius ED99, the mean
percentage adherence to canine corneocytes was 4.24% which was
significantly different to L. lactis carrying pOri23 alone
(P=0.001) (FIG. 7). L. lactis expressing SpsD and L. lactis
expressing SpsO adhered to ex vivo canine corneocytes (FIG. 7). The
increase in adherence was approaching significance for SpsD
(P=0.050), and was significant for SpsO when expressed in L. lactis
compared to L. lactis carrying pOri23 alone (P=0.004). Binding of
L. lactis expressing SpsL was not significantly different to L.
lactis carrying pOri23 alone (P=0.108), indicating that SpsL does
not promote adherence to canine corneocytes (FIG. 7).
Purified Recombinant Sps D, Sps L and Sps O Demonstrate Reactivity
with Canine Convalescent Serum.
[0128] Reactivity of recombinant A domain from Sps D, Sps L and Sps
O with canine convalescent serum from pyoderma cases was examined
by Western affinity blot analysis (FIG. 8). rSpsD, rSpsL and rSpsO
all crossreacted with IgG present in the canine serum (FIG. 8).
Pre-Incubation with Canine Convalescent Serum Inhibits
SpsL-Mediated Binding to Fibrinogen.
[0129] The ability of the reactive antibody present in convalescent
serum to inhibit SpsD and SpsL ligand binding was investigated
using a modified solid phase adherence assay. Prior to inoculation
into fibrinogen coated wells, PBS normalised cultures of L. lactis
expressing SpsD and SpsL were incubated for 2 h with doubling
dilutions of convalescent serum at 28.degree. C. (FIG. 9).
Convalescent serum inhibited binding of L. lactis expressing SpsL,
but not SpsD to canine fibrinogen, with complete inhibition at a
final concentration of 2% v/v (FIG. 9).
Discussion
[0130] In summary, genome-wide analysis of S. pseudintermedius ED99
revealed the presence of 17 genes encoding putative CWA proteins
based on typical MSCRAMM features. All MSCRAMM characteristics
searched for were identified for SpsD, SpsL, and SpsO, including a
signal sequence, a non-repeated A domain with two IgG-like folds,
tandemly repeated regions, and a C-terminal LPXTG-anchor motif.
Interestingly, SpsD, SpsL, and SpsO belong to different groups
based on Southern blot analysis, with SpsD being present in all SIG
members, SpsL in S. pseudintermedius only, and SpsO in only six of
the S. pseudintermedius strains investigated, and not in the other
SIG species tested. Based on in silico analysis and in vitro
expression data, SpsD, SpsL, and SpsO were selected for functional
characterisation.
[0131] All CWA proteins and in particular, SpsD, SpsL, and SpsO
could be employed in passive and active immunisation studies to
test their antigenic properties, either singular or in combination,
in a similar fashion as proposed for S. aureus ClfA (Josefsson et
al., 2001; Hall et al., 2003; Patti, 2004; Nanra et al., 2009).
Further, a combinatory vaccine of S. aureus surface proteins IsdA,
iron-regulated surface determinant protein B (IsdB), SdrD, and SdrE
has proven to be highly protective in a mouse infection model
(Stranger-Jones et al., 2006), demonstrating the promising
potential of vaccine preparations containing multiple
staphylococcal CWA proteins.
[0132] In addition, MSCRAMMs with known ligands could be targets of
anti-staphylococcal drug development, e.g. by generating synthetic
peptides based on the interacting ECM proteins, which antagonise
the MSCRAMM-host protein interaction, but do not interfere
profoundly with physiological processes in the host. An excellent
example is provided by Ganesh et al. (2008) who demonstrated that
synthetic peptides, based on the Fg-binding site for ClfA, hinder
the ClfA interaction, but do not block binding of the platelet
integrin .alpha..sub.IIb.beta..sub.3 to Fg. Recently,
Stranger-Jones et al screened the genome of the human pathogen S.
aureus for all genes predicted to encode CWA proteins, and
immunized mice with each protein to determine their capacity to
protect against lethal or invasive infection (Stranger-Jones et al,
2006). Four of the proteins were combined into a multiple protein
vaccine which induced high levels of protection against S. aureus
invasive disease of mice. These data have stimulated renewed
optimism in a vaccine for the prevention of human S. aureus
infections. A similar approach could be used to design an effective
vaccine for the prevention of S. pseudintermedius canine
pyoderma.
Example 2
[0133] Staphylococcus pseudintermedius Surface Protein Vaccination
Experiment.
[0134] S. pseudintermedius surface antigens were divided into 2
pools of 3 and 4 antigens, respectively. Vaccine pool 1 contained
antigens SpsC, IsaA, and SpsN and vaccine pool 2 contained SpsD A
domain, N2, N3 subdomains, SpsL A domain (SEQ ID NO: 37), and
SpsA.
[0135] Groups of 8 or 9 BalbC mice were vaccinated subcutaneously
with pool 1 or pool 2 or PBS, each with complete Freund's adjuvant,
followed by additional vaccinations at day 8 and day 23 with
incomplete Freund's adjuvant. On day 32, mice were challenged
through a subcutaneous route with 10.sup.7 cfu S. pseudintermedius
ED99. Mice were then examined for abscess formation, and weight
loss.
[0136] Mice vaccinated with pool 2 (comprising the protein having
amino acid sequence provided in SEQ ID NO: 37) had significantly
reduced lesion size (.about.50% reduction), and significantly
reduced weight loss (.about.50%) compared to PBS control mock
vaccinated animals.
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TABLE-US-00021 [0155] TABLE 1 Sequence homology of the 17 predicted
cell-wall-anchored proteins against known proteins in the public
domain. Putative CWA Identity Similarity protein Best Hit (BLAST)
(%) (%) SpsA LPXTG cell-wall surface anchor family protein of S.
aureus COL 31.2 56.9 SpsB RodA, a rod shape determining protein of
S. epidermidis ATCC 12228 69.7 87.8 SpsC bifunctional autolysin
precursor of S. epidermidis ATCC 12228 50.7 65.9 SpsD Fnbp protein
homolog of S. aureus Mu50 40.7 59.1 SpsE Fibrinogen binding protein
of S. epidermidis ATCC 12228 78.6 90.1 SpsF hypothetical protein,
similar to the putative cell-surface adhesin SdrF of S.
haemolyticus JCSC1435 52.8 69.3 SpsG hypothetical protein,
cell-wall surface anchor family of Streptococcus pneumoniae D39
47.7 63.6 SpsH Sdr-repeat family protein SdrH, S. aureus USA300
36.0 53.1 SpsI serine-aspartate rich, fibrinogen-binding, bone
sialoprotein-binding protein S. epidermidis ATCC 12228 37.3 55.5
SpsJ precursor of a serine-rich adhesin for platelets of S.
haemolyticus JCSC143S 52.2 61.2 SpsK IgG-binding protein of S.
aureus COL 50.4 71.1 SpsL Fnbp protein homolog of S. aureus Mu50
33.4 51.7 SpsM hypothetical protein, similar to the putative
cell-surface adhesin SdrF, S. haemolyticus JCSC1435 44.4 61.7 SpsN
probable exported protein of S. aureus RF122 38.0 60.0 SpsO
serine-aspartate repeat-containing protein C precursor of
Staphylococcus warneri L37603 50.0 68 SpsP LPXTG-motif cell wall
anchor domain of S. aureus JH9 60.6 74.3 SpsQ IgG-binding protein A
precursor of S. aureus MRSA252 57.0 71.7
TABLE-US-00022 TABLE 2 Main characteristics of the predicted CWA
proteins SpsD, SpsL, and SpsO of S. pseudintermedius ED99.
TYTFTDYVD- Putative latching Repeat Copy Amino MW Signal LPXTG
Ig-like fold.sup.b like motif sequence region number acids
(kDa).sup.a peptide motif (position) (position).sup.b
(position).sup.b (position) repeats SpsD 1031 115 36 aa LPDTG
167-320 aa RYRFMDYVN NNASGEG 867-959 aa 5 322-519 aa (267-275 aa)
(491-497 aa) SpsL 930 103 38 aa LPKTG 220-363 aa VYTFKDYVN NSASGSG
543-818 aa 7 364-531 aa (298-306 aa) (502-508 aa) SpsO 1846 198 44
aa LPNTG 339-492 aa TYTFTDYVD DKSTALG 661-1800 aa 96 487-659 aa
(424-432 aa) (635-641 aa) .sup. 97-216 aa.sup.c .sup. 4.sup.c aa =
amino acids; .sup.aMW = predicted approximate molecular weight in
kDa (kilo dalton); .sup.bwithin the A domain; .sup.cN-terminal
repeats
Sequence CWU 1
1
3811044DNAStaphylococcus pseudintermedius 1atggaaaaca aaaacttttt
tagtattcgt aaactatcta ttggtgtagg ttcttgctta 60atcgcgagtt ctttacttgt
aaacacgcca agttttgctg aagaaacaga taatgcgaac 120attaatgacg
cacaacaaaa cgccttttat gaaattttac atttgccaaa cttaactgaa
180gagcaacaaa atggattcat ccaaagtctt aaagatgatc caagtgtgag
caacgacatt 240ttagtagaag ctaagaaatt aaatgacact caagctaaac
ctgattacag tgaagcacaa 300caaaatgcat tttatgaaat tttacatttg
tcaaacttaa ctgaagagca acaaaatgga 360ttcatccaaa gtcttaaaga
tgatccaagt gtgagcaacg acattttagt agaagctaag 420aagttaaatg
acactcaagc taaacctgat tacagtgaag cacaacaaaa tgcattttat
480gaaattttac atttgtcaaa cttaactgaa gagcaacaaa atgggttcat
ccaaagcctt 540aaagatgatc caagtgtaag taaagaaatt ttagcagaag
ctaagaagtt aaatgatagt 600caagcaccta aagttgataa agctaaaaaa
actgacaaag ctgaagcgaa agcagatgat 660aaagctaaag gtgaagaagc
caaaaaatct gaagacaaaa aagatagcaa agcagataag 720gcaaaatcga
aaaacgctac acatgttgtt aaacctggtg aaactttaga taatattgct
780aaagatcatc atacaactgt tgataaaatt gctaaagata acaaaataaa
agataaaaat 840gtgattaaac taggtcaaaa acttgttgtt gataaacaaa
aagcaactca aggaaaacaa 900gaagctgtag cgaaaaatga agtgaaggct
ttacctaata ctggtgaaaa tgatgatatc 960gcattattca gcacaacagt
tgcgggtggc gtaagtatcg ctttaggttc attattatta 1020ggaagaaaca
gaaaaactag ctaa 10442347PRTStaphylococcus pseudintermedius 2Met Glu
Asn Lys Asn Phe Phe Ser Ile Arg Lys Leu Ser Ile Gly Val 1 5 10 15
Gly Ser Cys Leu Ile Ala Ser Ser Leu Leu Val Asn Thr Pro Ser Phe 20
25 30 Ala Glu Glu Thr Asp Asn Ala Asn Ile Asn Asp Ala Gln Gln Asn
Ala 35 40 45 Phe Tyr Glu Ile Leu His Leu Pro Asn Leu Thr Glu Glu
Gln Gln Asn 50 55 60 Gly Phe Ile Gln Ser Leu Lys Asp Asp Pro Ser
Val Ser Asn Asp Ile 65 70 75 80 Leu Val Glu Ala Lys Lys Leu Asn Asp
Thr Gln Ala Lys Pro Asp Tyr 85 90 95 Ser Glu Ala Gln Gln Asn Ala
Phe Tyr Glu Ile Leu His Leu Ser Asn 100 105 110 Leu Thr Glu Glu Gln
Gln Asn Gly Phe Ile Gln Ser Leu Lys Asp Asp 115 120 125 Pro Ser Val
Ser Asn Asp Ile Leu Val Glu Ala Lys Lys Leu Asn Asp 130 135 140 Thr
Gln Ala Lys Pro Asp Tyr Ser Glu Ala Gln Gln Asn Ala Phe Tyr 145 150
155 160 Glu Ile Leu His Leu Ser Asn Leu Thr Glu Glu Gln Gln Asn Gly
Phe 165 170 175 Ile Gln Ser Leu Lys Asp Asp Pro Ser Val Ser Lys Glu
Ile Leu Ala 180 185 190 Glu Ala Lys Lys Leu Asn Asp Ser Gln Ala Pro
Lys Val Asp Lys Ala 195 200 205 Lys Lys Thr Asp Lys Ala Glu Ala Lys
Ala Asp Asp Lys Ala Lys Gly 210 215 220 Glu Glu Ala Lys Lys Ser Glu
Asp Lys Lys Asp Ser Lys Ala Asp Lys 225 230 235 240 Ala Lys Ser Lys
Asn Ala Thr His Val Val Lys Pro Gly Glu Thr Leu 245 250 255 Asp Asn
Ile Ala Lys Asp His His Thr Thr Val Asp Lys Ile Ala Lys 260 265 270
Asp Asn Lys Ile Lys Asp Lys Asn Val Ile Lys Leu Gly Gln Lys Leu 275
280 285 Val Val Asp Lys Gln Lys Ala Thr Gln Gly Lys Gln Glu Ala Val
Ala 290 295 300 Lys Asn Glu Val Lys Ala Leu Pro Asn Thr Gly Glu Asn
Asp Asp Ile 305 310 315 320 Ala Leu Phe Ser Thr Thr Val Ala Gly Gly
Val Ser Ile Ala Leu Gly 325 330 335 Ser Leu Leu Leu Gly Arg Asn Arg
Lys Thr Ser 340 345 31389DNAStaphylococcus pseudintermedius
3atggaaaaca aaaacttttt cagcattcgt aaattatcaa ttggggtggg ttcatgttta
60atcgcgagct ctttacttgt gaatacacca agtttcgcag aagaaggaga taataacgca
120gaagcgcaac aaaacgcttt ctctgaggta gtaaaattac ctaaccttag
cgaagaacaa 180cgtaatggtt tcattcaaag ccttaaagat gatccaagta
caagtcaaga tgtgcttaat 240gaagctaaaa aattaaatga tagtcaagag
ggatctcaac ctgctcctga ttacagtgat 300gaacaacaaa atgcatttta
tgaaatttta caccttccaa acttaactga agaacaacgc 360aatggctata
ttcaaagtct taaagatgac ccaagtgtaa gcgctaatat tcttgttgaa
420gctaaaaata tgaatgttaa ccaaacacct acacaacctg cgccaagttt
cgatgaagcg 480caacaaaatg cattctatga gattgtaaac ttaccaaatc
ttactgaaga gcaacgtaac 540ggtttcatcc aaagccttaa agacgatcca
agtgtaagta aagatatcct tgttgaagct 600aaaaagttaa atgacagcca
agcaaaacct gattacagtg aagcgcaaca aaatgcattt 660tatgaaattt
tacaccttcc aaacttaact gaagaacaac gtaacggttt catccaaagc
720cttaaagacg atccgagtgt aagtagtgat attcttgctg aagctaagaa
attaaatgac 780agccaagcgc ctaaagaaga caacaacgta aaagacaata
attcaggtga aaacaaagct 840gaagacaaag gcaacaaaga aaacaaagct
gaagataaag gcagcaaaga agacaaagct 900gaagataaag gcagcaaaga
agacaaagct gaagataaag gcagcaaaga agacaaagct 960gaagataaag
gcagcaaaga agacaaagct gaagataaag gcagcataga agataaagct
1020aaagacaaag acaacaaaga aggcaaagct gcagacaaag gtatggacaa
agcgaaagat 1080gcaatgcatg tcgttcaacc tggtgaaaca gtagaaaaaa
ttgctaaagc taataacaca 1140actgtagaac aaatcgctaa agataatcat
ttagaagata aaaacatgat tttaccaggt 1200caaaaacttg ttgttgacaa
ccaaaaagca atgaaagaca gccaagaagc taaagcaaac 1260cacgaaatga
aagctttacc tgaaacaggt gaagaaaacg atatggcatt attcggtaca
1320tcacttacag gtggtcttag cttagcatta ggtttataca tcttaggacg
tggcagaaaa 1380acaaactaa 13894462PRTStaphylococcus pseudintermedius
4Met Glu Asn Lys Asn Phe Phe Ser Ile Arg Lys Leu Ser Ile Gly Val 1
5 10 15 Gly Ser Cys Leu Ile Ala Ser Ser Leu Leu Val Asn Thr Pro Ser
Phe 20 25 30 Ala Glu Glu Gly Asp Asn Asn Ala Glu Ala Gln Gln Asn
Ala Phe Ser 35 40 45 Glu Val Val Lys Leu Pro Asn Leu Ser Glu Glu
Gln Arg Asn Gly Phe 50 55 60 Ile Gln Ser Leu Lys Asp Asp Pro Ser
Thr Ser Gln Asp Val Leu Asn 65 70 75 80 Glu Ala Lys Lys Leu Asn Asp
Ser Gln Glu Gly Ser Gln Pro Ala Pro 85 90 95 Asp Tyr Ser Asp Glu
Gln Gln Asn Ala Phe Tyr Glu Ile Leu His Leu 100 105 110 Pro Asn Leu
Thr Glu Glu Gln Arg Asn Gly Tyr Ile Gln Ser Leu Lys 115 120 125 Asp
Asp Pro Ser Val Ser Ala Asn Ile Leu Val Glu Ala Lys Asn Met 130 135
140 Asn Val Asn Gln Thr Pro Thr Gln Pro Ala Pro Ser Phe Asp Glu Ala
145 150 155 160 Gln Gln Asn Ala Phe Tyr Glu Ile Val Asn Leu Pro Asn
Leu Thr Glu 165 170 175 Glu Gln Arg Asn Gly Phe Ile Gln Ser Leu Lys
Asp Asp Pro Ser Val 180 185 190 Ser Lys Asp Ile Leu Val Glu Ala Lys
Lys Leu Asn Asp Ser Gln Ala 195 200 205 Lys Pro Asp Tyr Ser Glu Ala
Gln Gln Asn Ala Phe Tyr Glu Ile Leu 210 215 220 His Leu Pro Asn Leu
Thr Glu Glu Gln Arg Asn Gly Phe Ile Gln Ser 225 230 235 240 Leu Lys
Asp Asp Pro Ser Val Ser Ser Asp Ile Leu Ala Glu Ala Lys 245 250 255
Lys Leu Asn Asp Ser Gln Ala Pro Lys Glu Asp Asn Asn Val Lys Asp 260
265 270 Asn Asn Ser Gly Glu Asn Lys Ala Glu Asp Lys Gly Asn Lys Glu
Asn 275 280 285 Lys Ala Glu Asp Lys Gly Ser Lys Glu Asp Lys Ala Glu
Asp Lys Gly 290 295 300 Ser Lys Glu Asp Lys Ala Glu Asp Lys Gly Ser
Lys Glu Asp Lys Ala 305 310 315 320 Glu Asp Lys Gly Ser Lys Glu Asp
Lys Ala Glu Asp Lys Gly Ser Ile 325 330 335 Glu Asp Lys Ala Lys Asp
Lys Asp Asn Lys Glu Gly Lys Ala Ala Asp 340 345 350 Lys Gly Met Asp
Lys Ala Lys Asp Ala Met His Val Val Gln Pro Gly 355 360 365 Glu Thr
Val Glu Lys Ile Ala Lys Ala Asn Asn Thr Thr Val Glu Gln 370 375 380
Ile Ala Lys Asp Asn His Leu Glu Asp Lys Asn Met Ile Leu Pro Gly 385
390 395 400 Gln Lys Leu Val Val Asp Asn Gln Lys Ala Met Lys Asp Ser
Gln Glu 405 410 415 Ala Lys Ala Asn His Glu Met Lys Ala Leu Pro Glu
Thr Gly Glu Glu 420 425 430 Asn Asp Met Ala Leu Phe Gly Thr Ser Leu
Thr Gly Gly Leu Ser Leu 435 440 445 Ala Leu Gly Leu Tyr Ile Leu Gly
Arg Gly Arg Lys Thr Asn 450 455 460 52793DNAStaphylococcus
pseudintermedius 5gtgtacaaaa atgaagaaga aaagcattca ataagaaagt
tatctatagg agccgcatct 60gtcattgttg ggggactcat gtatggtgtt ttgggaaatg
atgaagctca agcgaatgaa 120gatgtcactg aaacaactgg gagaaattca
gtgacaacgc aagcttctga gcaacatttg 180caagtggaag cagtacctca
agaaggcaat aatgtaaatg tatcctctgt aaaagtacct 240acgaatacgg
caacgcaagc acaagaagat gttgcaagtg tatccgatgt taaagcacat
300gctgatgatg cattacaagt acaagaaagt agtcatactg atggtgtttc
ttcagaattc 360aagcaggaga cagcttatgc gaatcctcaa acagctgaga
cagttaaacc taatagtgaa 420gcagtgcatc agtctgaata cgaggataag
caaaaacccg tatcatctag ccgcaaagaa 480gatgagacta tgcttcagca
gcaacaagtt gaagccaaaa atgttgtgag tgcggaggaa 540gtgtctaaag
aagaaaatac tcaagtgatg caatcccctc aagacgttga acaacatgta
600ggtggtaaag atatctctaa tgaggttgta gtggatagga gtgatatcaa
aggatttaac 660agcgaaacta ctattcgacc tcatcaggga caaggtggta
ggttgaatta tcaattaaag 720tttcctagca atgtaaagcc aggcgatcag
tttactataa aattatctga caatatcaat 780acacatggtg tttctgttga
aagaaccgca ccgagaatca tggctaaaaa tactgaaggt 840gcgacggatg
taattgctga aggtctagtg ttggaagatg gtaaaaccat cgtatataca
900tttaaagact atgtaaatgg caagcaaaat ttgactgctg agttatcagt
gagctatttc 960gtaagtccgg aaaaagtctt gactactggg acacaaacat
tcacgacgat gatcggtaat 1020cattcaacgc aatccaatat tgacgtttat
tatgataata gtcattatgt agatggacgt 1080atttcgcaag tgaacaaaaa
agaagctaaa tttcaacaaa tagcatacat taaccctaat 1140ggctatttaa
atggcagggg gacaattgca gttaatggtg aagtggtcag tggtacgact
1200aaagacttaa tgcaacctac agtgcgtgta tatcaatata aaggacaagg
tgttcctcct 1260gaaagtatta ctatagaccc taatatgtgg gaagaaatca
gcataaacga tactatggta 1320agaaaatatg atggtggcta tagcttgaat
ctggatacca gcaagaatca aaaatatgcc 1380atctattatg aaggggcata
tgatgcgcaa gctgacacac tgttgtatag aacatatata 1440cagtcattaa
acagttacta tccgttcagt taccaaaaaa tgaacggtgt gaagttttac
1500gaaaacagtg cgagtggaag cggtgagttg aaaccgaaac cacctgaaca
accaaaacca 1560gaacctgaaa ttcaagctga tgtagtagat attattgaag
atagccatgt gattgatata 1620ggatggaata cagcagttgg agaagaaagt
ggagcaaacc aaggccctca agaagaaatc 1680acggaaaatc acgacatcga
agtcattgag gaaaacaact tggtggaaat gacagaagat 1740acagcagttg
gagaagaaag tggagcaaac caaggccctc aagaagaaat cacggaaaat
1800cacgacatcg aagtcattga agaaaacaac ttagtggaaa tgacagaaga
tacagcgttg 1860gaagaagaaa gtggagcaaa tcaaggtcct caagaagaga
tcacagaaaa ccacgatatc 1920gaagtcattg aagaaaacaa cttggtggaa
atgacagaag atacagcgtt ggaagaagaa 1980agtggagcaa atcaaggtcc
tcaagaagag atcacagaaa accacgacat cgaagtcatt 2040gaagaaaata
acttagtaga aatgacagaa gatacagcag ttggagaaga aagtggagca
2100aatcaaggtc ctcaagaaga gatcacagaa aaccacgata tcgaagtcat
tgaggaaaac 2160aacttagtgg aaatgacaga agatacagca gttggagaag
aaagtggagc aaaccaaggt 2220cctcaagaag aaatcacgga aaatcacgac
atcgaagtca ttgaagaaaa caacttggtg 2280gaaatgacag aagatacagc
gttggaagaa gaaagtggag caaatcaagg tcctcaagaa 2340gagatcacag
aaaaccacaa catcgaagtc attgaagaaa acaacttggt ggaaatgaca
2400gaagatacag cagttggaga agaaagtgga gcaaacccag gacctcaaga
agaagtaaca 2460gagaatcaac ctcagcaaga agaaatcatg gaaaaccaag
aagtcgaaaa gaaaggcgat 2520agtaacttgg tagaaagtac aaaaactcca
aaggccgaag aatcagttag cgttcagcca 2580actttagaag acaaaaacac
aaagaaccac gttaacacag tagtagtgaa tacgaaggta 2640tctgaagtta
aagaaaagga tccccaccat acaaaagcac taccagatac ggggacaacc
2700tctcgaagtc attccatgat gattcctctc cttcttgttg ctgggtcagt
agtgttgtta 2760cgtcgaaaga aaaagcatag taaggtgaat taa
27936930PRTStaphylococcus pseudintermedius 6Val Tyr Lys Asn Glu Glu
Glu Lys His Ser Ile Arg Lys Leu Ser Ile 1 5 10 15 Gly Ala Ala Ser
Val Ile Val Gly Gly Leu Met Tyr Gly Val Leu Gly 20 25 30 Asn Asp
Glu Ala Gln Ala Asn Glu Asp Val Thr Glu Thr Thr Gly Arg 35 40 45
Asn Ser Val Thr Thr Gln Ala Ser Glu Gln His Leu Gln Val Glu Ala 50
55 60 Val Pro Gln Glu Gly Asn Asn Val Asn Val Ser Ser Val Lys Val
Pro 65 70 75 80 Thr Asn Thr Ala Thr Gln Ala Gln Glu Asp Val Ala Ser
Val Ser Asp 85 90 95 Val Lys Ala His Ala Asp Asp Ala Leu Gln Val
Gln Glu Ser Ser His 100 105 110 Thr Asp Gly Val Ser Ser Glu Phe Lys
Gln Glu Thr Ala Tyr Ala Asn 115 120 125 Pro Gln Thr Ala Glu Thr Val
Lys Pro Asn Ser Glu Ala Val His Gln 130 135 140 Ser Glu Tyr Glu Asp
Lys Gln Lys Pro Val Ser Ser Ser Arg Lys Glu 145 150 155 160 Asp Glu
Thr Met Leu Gln Gln Gln Gln Val Glu Ala Lys Asn Val Val 165 170 175
Ser Ala Glu Glu Val Ser Lys Glu Glu Asn Thr Gln Val Met Gln Ser 180
185 190 Pro Gln Asp Val Glu Gln His Val Gly Gly Lys Asp Ile Ser Asn
Glu 195 200 205 Val Val Val Asp Arg Ser Asp Ile Lys Gly Phe Asn Ser
Glu Thr Thr 210 215 220 Ile Arg Pro His Gln Gly Gln Gly Gly Arg Leu
Asn Tyr Gln Leu Lys 225 230 235 240 Phe Pro Ser Asn Val Lys Pro Gly
Asp Gln Phe Thr Ile Lys Leu Ser 245 250 255 Asp Asn Ile Asn Thr His
Gly Val Ser Val Glu Arg Thr Ala Pro Arg 260 265 270 Ile Met Ala Lys
Asn Thr Glu Gly Ala Thr Asp Val Ile Ala Glu Gly 275 280 285 Leu Val
Leu Glu Asp Gly Lys Thr Ile Val Tyr Thr Phe Lys Asp Tyr 290 295 300
Val Asn Gly Lys Gln Asn Leu Thr Ala Glu Leu Ser Val Ser Tyr Phe 305
310 315 320 Val Ser Pro Glu Lys Val Leu Thr Thr Gly Thr Gln Thr Phe
Thr Thr 325 330 335 Met Ile Gly Asn His Ser Thr Gln Ser Asn Ile Asp
Val Tyr Tyr Asp 340 345 350 Asn Ser His Tyr Val Asp Gly Arg Ile Ser
Gln Val Asn Lys Lys Glu 355 360 365 Ala Lys Phe Gln Gln Ile Ala Tyr
Ile Asn Pro Asn Gly Tyr Leu Asn 370 375 380 Gly Arg Gly Thr Ile Ala
Val Asn Gly Glu Val Val Ser Gly Thr Thr 385 390 395 400 Lys Asp Leu
Met Gln Pro Thr Val Arg Val Tyr Gln Tyr Lys Gly Gln 405 410 415 Gly
Val Pro Pro Glu Ser Ile Thr Ile Asp Pro Asn Met Trp Glu Glu 420 425
430 Ile Ser Ile Asn Asp Thr Met Val Arg Lys Tyr Asp Gly Gly Tyr Ser
435 440 445 Leu Asn Leu Asp Thr Ser Lys Asn Gln Lys Tyr Ala Ile Tyr
Tyr Glu 450 455 460 Gly Ala Tyr Asp Ala Gln Ala Asp Thr Leu Leu Tyr
Arg Thr Tyr Ile 465 470 475 480 Gln Ser Leu Asn Ser Tyr Tyr Pro Phe
Ser Tyr Gln Lys Met Asn Gly 485 490 495 Val Lys Phe Tyr Glu Asn Ser
Ala Ser Gly Ser Gly Glu Leu Lys Pro 500 505 510 Lys Pro Pro Glu Gln
Pro Lys Pro Glu Pro Glu Ile Gln Ala Asp Val 515 520 525 Val Asp Ile
Ile Glu Asp Ser His Val Ile Asp Ile Gly Trp Asn Thr 530 535 540 Ala
Val Gly Glu Glu Ser Gly Ala Asn Gln Gly Pro Gln Glu Glu Ile 545 550
555 560 Thr Glu Asn His Asp Ile Glu Val Ile Glu Glu Asn Asn Leu Val
Glu 565 570 575 Met Thr Glu Asp Thr Ala Val Gly Glu Glu Ser Gly Ala
Asn Gln Gly 580 585 590 Pro Gln Glu Glu Ile Thr Glu Asn His Asp Ile
Glu Val Ile Glu Glu 595 600 605 Asn Asn Leu Val Glu Met Thr Glu Asp
Thr Ala Leu Glu Glu Glu Ser 610 615 620 Gly Ala Asn Gln Gly Pro Gln
Glu Glu Ile Thr Glu Asn His Asp Ile 625 630 635 640 Glu Val Ile Glu
Glu Asn Asn Leu Val Glu Met
Thr Glu Asp Thr Ala 645 650 655 Leu Glu Glu Glu Ser Gly Ala Asn Gln
Gly Pro Gln Glu Glu Ile Thr 660 665 670 Glu Asn His Asp Ile Glu Val
Ile Glu Glu Asn Asn Leu Val Glu Met 675 680 685 Thr Glu Asp Thr Ala
Val Gly Glu Glu Ser Gly Ala Asn Gln Gly Pro 690 695 700 Gln Glu Glu
Ile Thr Glu Asn His Asp Ile Glu Val Ile Glu Glu Asn 705 710 715 720
Asn Leu Val Glu Met Thr Glu Asp Thr Ala Val Gly Glu Glu Ser Gly 725
730 735 Ala Asn Gln Gly Pro Gln Glu Glu Ile Thr Glu Asn His Asp Ile
Glu 740 745 750 Val Ile Glu Glu Asn Asn Leu Val Glu Met Thr Glu Asp
Thr Ala Leu 755 760 765 Glu Glu Glu Ser Gly Ala Asn Gln Gly Pro Gln
Glu Glu Ile Thr Glu 770 775 780 Asn His Asn Ile Glu Val Ile Glu Glu
Asn Asn Leu Val Glu Met Thr 785 790 795 800 Glu Asp Thr Ala Val Gly
Glu Glu Ser Gly Ala Asn Pro Gly Pro Gln 805 810 815 Glu Glu Val Thr
Glu Asn Gln Pro Gln Gln Glu Glu Ile Met Glu Asn 820 825 830 Gln Glu
Val Glu Lys Lys Gly Asp Ser Asn Leu Val Glu Ser Thr Lys 835 840 845
Thr Pro Lys Ala Glu Glu Ser Val Ser Val Gln Pro Thr Leu Glu Asp 850
855 860 Lys Asn Thr Lys Asn His Val Asn Thr Val Val Val Asn Thr Lys
Val 865 870 875 880 Ser Glu Val Lys Glu Lys Asp Pro His His Thr Lys
Ala Leu Pro Asp 885 890 895 Thr Gly Thr Thr Ser Arg Ser His Ser Met
Met Ile Pro Leu Leu Leu 900 905 910 Val Ala Gly Ser Val Val Leu Leu
Arg Arg Lys Lys Lys His Ser Lys 915 920 925 Val Asn 930
74323DNAStaphylococcus pseudintermedius 7atgaataaat caagaactaa
acattttaat tttttatcaa aacgtcagaa tcggtatgct 60attcgccact tttcagctgg
tactgtgtca gtgcttgtag gagcagcttt cttgctaggt 120gtccatacga
gtgatgcatc tgctgcagaa caagatcaaa catctgaagc aaagcaaaac
180ctctttgatg cttccgctat ttttggcgct ttaacagaga cgaacgaaaa
ggtagcacaa 240gtgacgccaa cagaaaaaaa tctttcatca gttgaagaaa
tgagagataa aggcgcaact 300ggaaatggac catcaataac atcactacaa
actgtagaac aaaataatgc agtacaacct 360acagcaacac ctattaatga
cacagaaaat tcaaccgaag cccctatgaa agaacaatcg 420aatgatgcac
aaacgactga cgaaagtaac aatgccactc agaaaaataa tactgaaccc
480caagcaaaca atgaaatatc agcgcgtaat gcaaaaacaa cagcatattt
aacaagtgaa 540acctttacaa cagcaacgtc tacaactgat atgcctacac
agaaacaaga atatccatct 600ttagaaaatc caacaaatca atcgcaaacg
aacagagcac aaccaccaac aatggaagca 660cccaaactgg cagaaggatt
agacaatcta ttaaaaaaat caactttcga aagtatgtac 720gtgacaaaaa
gaaatcaatt tgacaaagag acggcttcta aaacaaaagc atggccgagt
780gatgttgttc cagaaaatca agtagagata cttgctgatg caattcaaaa
tggctatatc 840aaatctgtaa atgatgtgac caataaagca catacgttat
ctggacgtgc atggatgtta 900gaccacggaa caccaacgac aatagctaat
ggtttaacac ctgttccaga gggcactaaa 960gtttatttgc ggtggataga
tcaagatggt gccacttcgc caatgtatac agcaaaaacg 1020acaagtagat
taagcgctgc ggatggtaat caagtgggtc caggtgctta tgctttcgat
1080ttacgcacag gttggataga tgctaaagga aaacaccacg tatatagagc
agtaaagggt 1140caatattata aaatatggat caatgatttt agaactaaag
acggtaatat cgctacaatg 1200ttacgtgttg caggaggata tgttccggga
acgtacgtgg attctgtgac atacaacaat 1260atgggccaat ttccattaat
tggtacaaat atgcaacgta caggtatctt tatgacaacg 1320ataccttcag
aaaaatattt aatatcaaaa cattacgtga aagatacaaa aggtgctgca
1380gcaaatccag ccgtcacgat aattgaaaat aactttgtga gcggcaaagt
ttggatagaa 1440acaggtgctg gagattatgt gaactcagcg acaggtccaa
accacaatgc gaaagatgtc 1500gttgcctctg gatacaaagt ggtcatgtca
tcattaacag atcaaggtgc taaagcctac 1560gatgcgcaag tcaatcgctt
gccgaagaaa gatcgagcag aagcagcacg tcaattatta 1620ataaaacatc
cagaatatat cgcagcaact gtagaaggga taacgaatga gtgggggaga
1680tatacattgc gtttccctaa aggcacattc aacaaagacc atctttacgg
ttacgtattg 1740gattttgatg gtgaaattgt aaaaacttat tcaggtttta
cttcaccaga gttccggaga 1800ccgaattata atttgaccgt tacaccgcaa
acagctccct attatagacc cgttcgacgt 1860gcatgggtca atgttaattt
tgcggttatt gaagcaccac aatctcaaat cgaaataaaa 1920gaatttgatg
caacctctaa ccctgcgcat cgtgggcaaa cagcaactat tgatatcata
1980ggtatgccta aaacttcatt acttacacgt gtacaatgga aagattcatc
gggcagtatt 2040gttgaggata gtggtcctgt ttttacggaa gaagaggctg
aacatatagc ggaatttgta 2100ataccgtcta gcgcaaaatc aggcgaagtg
tatactgtac aactcgtggt aggtaatcat 2160atcgtagctt cggactctct
tattgtacat gtcaatgaag aagcggcgac atatcatccg 2220atatacccat
cgacaacagt agaatcaggt caaagagtaa cgattccagc acctaagaat
2280atggatggca aacctttact agatggcaca acttttgaaa aaggtcatca
cgtaccaact 2340tgggctttag tgaatggtga tggctcgatt acagtaaaac
ctggagaaaa agtagcagag 2400ggtgagtatg atattccagt gattgtgaca
tatccagatg gttctaaaaa cacaatcttt 2460gcacctgtga ccgttgaaga
aaaacaacca atggcatcgc aatatgagcc aataacaact 2520ggagtatcga
aaccatttgg aaacccagta atgccaactg atgtaacaga ttcaattcaa
2580gtaccgaact atccattgga agggcaacaa ccgacagtaa cagtggatga
tgaaagtcaa 2640ttaccagatg gaacaacaga aggttacaag gatatagatg
taacagtgac atacccagac 2700ggaacaaagg atcgtgtcaa agttccagtc
gtaacggaac aacaattaga tagtgataaa 2760tatgatccgg tcgcaacagg
tatcttgaaa ccgtttggta ctccaacaac agaggaagac 2820gttataaaat
tagtggagat accgaaatat ccaacagact taacacaacc aaaagtaaca
2880gtgacggttc caaatacttt accggatggg caaacgccag gtaaagtaga
cgttgatgtg 2940acagtaacgt atccagatgg ttccacagat cacatttcag
ttccagtttg gacaaacaag 3000catctggata aagacaaata taacccaata
acgactgggg tatcgaaacc atttggaatc 3060ccagtaacgc caactgatgt
aacagattca attcaagtac cgaactatcc attggaaggg 3120caacaactga
cagtaacagt ggatgatgaa acacaattac cagatggaac aacagaaggt
3180cacaaggata tagatgtaac agtgacatac ccagacggaa caaaggatca
tatcaaagtt 3240ccagtcgtaa cggaaaaaca atcagataat gaaaaatatg
agccaacaac taacggaatc 3300acgaaaaagt acggtatccc tacgacagag
gatgaagtga tagatatagt tcgaattcca 3360tattttccag tagatggcgt
gcaacctatt gtaacggtaa atgatcctag actattgcca 3420aatggtcaaa
aagaaggtca aatcaatgtt ccagtcacag tgacgtatcc ggatggcaca
3480aaagatctca tgacagttcc ggttattaca ggtaagcaag cagaaaatga
aaaatacgat 3540ccaatcacat taggagtaac taaagattat ggtgatccta
caactgcaaa cgatgtgaca 3600aagtcaatcc aaataccaac atatccagca
ggtggcgaac aaccaatcgc aacagcggat 3660gatgaaagtc aattaccgga
tggcacagta gaaggtaaag tggatattcc agtcacagtg 3720acgtatccgg
atggtactca ggatcatatc actgtcccag tatttaccaa tcaacaacga
3780gataatcaaa aagccagtaa agctgtgacg aaaatacatg gtatatcggt
aacaggcact 3840gatatgacag atactaagaa aaatcataac tatccagcag
gtggtgaaca acctaaagtt 3900actgtgaaag atgacgatca attatcagag
ggtaaagtcg attcaacagt gggtgcggat 3960aatgtgacaa ctacagatga
tttatcaagc gtaactgcgg tatctcatgg tcatcaaaca 4020agtgtacaaa
caacaaaaga gaaccaatca gtgcatgatg aagaggtgac gatcccaaca
4080gttgcacatg tgtctacaat aatgacaggt gtggtaaagg gtgagcaaga
agcgacggat 4140atcgtggcta gaccacatgt tgaaacaact caactcccat
caatttcagc tcaagcaaca 4200gttaaaaaac taccagaaac gggtgaaaac
aatgaacaat caggtgtttt attaggtgga 4260tttattgcgt tcatgggtag
cttactttta ttcggcagac gtcgcaaacc aaagaaagat 4320taa
432381440PRTStaphylococcus pseudintermedius 8Met Asn Lys Ser Arg
Thr Lys His Phe Asn Phe Leu Ser Lys Arg Gln 1 5 10 15 Asn Arg Tyr
Ala Ile Arg His Phe Ser Ala Gly Thr Val Ser Val Leu 20 25 30 Val
Gly Ala Ala Phe Leu Leu Gly Val His Thr Ser Asp Ala Ser Ala 35 40
45 Ala Glu Gln Asp Gln Thr Ser Glu Ala Lys Gln Asn Leu Phe Asp Ala
50 55 60 Ser Ala Ile Phe Gly Ala Leu Thr Glu Thr Asn Glu Lys Val
Ala Gln 65 70 75 80 Val Thr Pro Thr Glu Lys Asn Leu Ser Ser Val Glu
Glu Met Arg Asp 85 90 95 Lys Gly Ala Thr Gly Asn Gly Pro Ser Ile
Thr Ser Leu Gln Thr Val 100 105 110 Glu Gln Asn Asn Ala Val Gln Pro
Thr Ala Thr Pro Ile Asn Asp Thr 115 120 125 Glu Asn Ser Thr Glu Ala
Pro Met Lys Glu Gln Ser Asn Asp Ala Gln 130 135 140 Thr Thr Asp Glu
Ser Asn Asn Ala Thr Gln Lys Asn Asn Thr Glu Pro 145 150 155 160 Gln
Ala Asn Asn Glu Ile Ser Ala Arg Asn Ala Lys Thr Thr Ala Tyr 165 170
175 Leu Thr Ser Glu Thr Phe Thr Thr Ala Thr Ser Thr Thr Asp Met Pro
180 185 190 Thr Gln Lys Gln Glu Tyr Pro Ser Leu Glu Asn Pro Thr Asn
Gln Ser 195 200 205 Gln Thr Asn Arg Ala Gln Pro Pro Thr Met Glu Ala
Pro Lys Leu Ala 210 215 220 Glu Gly Leu Asp Asn Leu Leu Lys Lys Ser
Thr Phe Glu Ser Met Tyr 225 230 235 240 Val Thr Lys Arg Asn Gln Phe
Asp Lys Glu Thr Ala Ser Lys Thr Lys 245 250 255 Ala Trp Pro Ser Asp
Val Val Pro Glu Asn Gln Val Glu Ile Leu Ala 260 265 270 Asp Ala Ile
Gln Asn Gly Tyr Ile Lys Ser Val Asn Asp Val Thr Asn 275 280 285 Lys
Ala His Thr Leu Ser Gly Arg Ala Trp Met Leu Asp His Gly Thr 290 295
300 Pro Thr Thr Ile Ala Asn Gly Leu Thr Pro Val Pro Glu Gly Thr Lys
305 310 315 320 Val Tyr Leu Arg Trp Ile Asp Gln Asp Gly Ala Thr Ser
Pro Met Tyr 325 330 335 Thr Ala Lys Thr Thr Ser Arg Leu Ser Ala Ala
Asp Gly Asn Gln Val 340 345 350 Gly Pro Gly Ala Tyr Ala Phe Asp Leu
Arg Thr Gly Trp Ile Asp Ala 355 360 365 Lys Gly Lys His His Val Tyr
Arg Ala Val Lys Gly Gln Tyr Tyr Lys 370 375 380 Ile Trp Ile Asn Asp
Phe Arg Thr Lys Asp Gly Asn Ile Ala Thr Met 385 390 395 400 Leu Arg
Val Ala Gly Gly Tyr Val Pro Gly Thr Tyr Val Asp Ser Val 405 410 415
Thr Tyr Asn Asn Met Gly Gln Phe Pro Leu Ile Gly Thr Asn Met Gln 420
425 430 Arg Thr Gly Ile Phe Met Thr Thr Ile Pro Ser Glu Lys Tyr Leu
Ile 435 440 445 Ser Lys His Tyr Val Lys Asp Thr Lys Gly Ala Ala Ala
Asn Pro Ala 450 455 460 Val Thr Ile Ile Glu Asn Asn Phe Val Ser Gly
Lys Val Trp Ile Glu 465 470 475 480 Thr Gly Ala Gly Asp Tyr Val Asn
Ser Ala Thr Gly Pro Asn His Asn 485 490 495 Ala Lys Asp Val Val Ala
Ser Gly Tyr Lys Val Val Met Ser Ser Leu 500 505 510 Thr Asp Gln Gly
Ala Lys Ala Tyr Asp Ala Gln Val Asn Arg Leu Pro 515 520 525 Lys Lys
Asp Arg Ala Glu Ala Ala Arg Gln Leu Leu Ile Lys His Pro 530 535 540
Glu Tyr Ile Ala Ala Thr Val Glu Gly Ile Thr Asn Glu Trp Gly Arg 545
550 555 560 Tyr Thr Leu Arg Phe Pro Lys Gly Thr Phe Asn Lys Asp His
Leu Tyr 565 570 575 Gly Tyr Val Leu Asp Phe Asp Gly Glu Ile Val Lys
Thr Tyr Ser Gly 580 585 590 Phe Thr Ser Pro Glu Phe Arg Arg Pro Asn
Tyr Asn Leu Thr Val Thr 595 600 605 Pro Gln Thr Ala Pro Tyr Tyr Arg
Pro Val Arg Arg Ala Trp Val Asn 610 615 620 Val Asn Phe Ala Val Ile
Glu Ala Pro Gln Ser Gln Ile Glu Ile Lys 625 630 635 640 Glu Phe Asp
Ala Thr Ser Asn Pro Ala His Arg Gly Gln Thr Ala Thr 645 650 655 Ile
Asp Ile Ile Gly Met Pro Lys Thr Ser Leu Leu Thr Arg Val Gln 660 665
670 Trp Lys Asp Ser Ser Gly Ser Ile Val Glu Asp Ser Gly Pro Val Phe
675 680 685 Thr Glu Glu Glu Ala Glu His Ile Ala Glu Phe Val Ile Pro
Ser Ser 690 695 700 Ala Lys Ser Gly Glu Val Tyr Thr Val Gln Leu Val
Val Gly Asn His 705 710 715 720 Ile Val Ala Ser Asp Ser Leu Ile Val
His Val Asn Glu Glu Ala Ala 725 730 735 Thr Tyr His Pro Ile Tyr Pro
Ser Thr Thr Val Glu Ser Gly Gln Arg 740 745 750 Val Thr Ile Pro Ala
Pro Lys Asn Met Asp Gly Lys Pro Leu Leu Asp 755 760 765 Gly Thr Thr
Phe Glu Lys Gly His His Val Pro Thr Trp Ala Leu Val 770 775 780 Asn
Gly Asp Gly Ser Ile Thr Val Lys Pro Gly Glu Lys Val Ala Glu 785 790
795 800 Gly Glu Tyr Asp Ile Pro Val Ile Val Thr Tyr Pro Asp Gly Ser
Lys 805 810 815 Asn Thr Ile Phe Ala Pro Val Thr Val Glu Glu Lys Gln
Pro Met Ala 820 825 830 Ser Gln Tyr Glu Pro Ile Thr Thr Gly Val Ser
Lys Pro Phe Gly Asn 835 840 845 Pro Val Met Pro Thr Asp Val Thr Asp
Ser Ile Gln Val Pro Asn Tyr 850 855 860 Pro Leu Glu Gly Gln Gln Pro
Thr Val Thr Val Asp Asp Glu Ser Gln 865 870 875 880 Leu Pro Asp Gly
Thr Thr Glu Gly Tyr Lys Asp Ile Asp Val Thr Val 885 890 895 Thr Tyr
Pro Asp Gly Thr Lys Asp Arg Val Lys Val Pro Val Val Thr 900 905 910
Glu Gln Gln Leu Asp Ser Asp Lys Tyr Asp Pro Val Ala Thr Gly Ile 915
920 925 Leu Lys Pro Phe Gly Thr Pro Thr Thr Glu Glu Asp Val Ile Lys
Leu 930 935 940 Val Glu Ile Pro Lys Tyr Pro Thr Asp Leu Thr Gln Pro
Lys Val Thr 945 950 955 960 Val Thr Val Pro Asn Thr Leu Pro Asp Gly
Gln Thr Pro Gly Lys Val 965 970 975 Asp Val Asp Val Thr Val Thr Tyr
Pro Asp Gly Ser Thr Asp His Ile 980 985 990 Ser Val Pro Val Trp Thr
Asn Lys His Leu Asp Lys Asp Lys Tyr Asn 995 1000 1005 Pro Ile Thr
Thr Gly Val Ser Lys Pro Phe Gly Ile Pro Val Thr 1010 1015 1020 Pro
Thr Asp Val Thr Asp Ser Ile Gln Val Pro Asn Tyr Pro Leu 1025 1030
1035 Glu Gly Gln Gln Leu Thr Val Thr Val Asp Asp Glu Thr Gln Leu
1040 1045 1050 Pro Asp Gly Thr Thr Glu Gly His Lys Asp Ile Asp Val
Thr Val 1055 1060 1065 Thr Tyr Pro Asp Gly Thr Lys Asp His Ile Lys
Val Pro Val Val 1070 1075 1080 Thr Glu Lys Gln Ser Asp Asn Glu Lys
Tyr Glu Pro Thr Thr Asn 1085 1090 1095 Gly Ile Thr Lys Lys Tyr Gly
Ile Pro Thr Thr Glu Asp Glu Val 1100 1105 1110 Ile Asp Ile Val Arg
Ile Pro Tyr Phe Pro Val Asp Gly Val Gln 1115 1120 1125 Pro Ile Val
Thr Val Asn Asp Pro Arg Leu Leu Pro Asn Gly Gln 1130 1135 1140 Lys
Glu Gly Gln Ile Asn Val Pro Val Thr Val Thr Tyr Pro Asp 1145 1150
1155 Gly Thr Lys Asp Leu Met Thr Val Pro Val Ile Thr Gly Lys Gln
1160 1165 1170 Ala Glu Asn Glu Lys Tyr Asp Pro Ile Thr Leu Gly Val
Thr Lys 1175 1180 1185 Asp Tyr Gly Asp Pro Thr Thr Ala Asn Asp Val
Thr Lys Ser Ile 1190 1195 1200 Gln Ile Pro Thr Tyr Pro Ala Gly Gly
Glu Gln Pro Ile Ala Thr 1205 1210 1215 Ala Asp Asp Glu Ser Gln Leu
Pro Asp Gly Thr Val Glu Gly Lys 1220 1225 1230 Val Asp Ile Pro Val
Thr Val Thr Tyr Pro Asp Gly Thr Gln Asp 1235 1240 1245 His Ile Thr
Val Pro Val Phe Thr Asn Gln Gln Arg Asp Asn Gln 1250 1255 1260 Lys
Ala Ser Lys Ala Val Thr Lys Ile His Gly Ile Ser Val Thr 1265 1270
1275 Gly Thr Asp Met Thr Asp Thr Lys Lys Asn His Asn Tyr Pro Ala
1280 1285 1290 Gly Gly Glu Gln Pro Lys Val Thr Val Lys Asp Asp Asp
Gln Leu 1295 1300 1305 Ser Glu Gly Lys Val Asp Ser Thr Val Gly Ala
Asp Asn Val Thr 1310 1315 1320
Thr Thr Asp Asp Leu Ser Ser Val Thr Ala Val Ser His Gly His 1325
1330 1335 Gln Thr Ser Val Gln Thr Thr Lys Glu Asn Gln Ser Val His
Asp 1340 1345 1350 Glu Glu Val Thr Ile Pro Thr Val Ala His Val Ser
Thr Ile Met 1355 1360 1365 Thr Gly Val Val Lys Gly Glu Gln Glu Ala
Thr Asp Ile Val Ala 1370 1375 1380 Arg Pro His Val Glu Thr Thr Gln
Leu Pro Ser Ile Ser Ala Gln 1385 1390 1395 Ala Thr Val Lys Lys Leu
Pro Glu Thr Gly Glu Asn Asn Glu Gln 1400 1405 1410 Ser Gly Val Leu
Leu Gly Gly Phe Ile Ala Phe Met Gly Ser Leu 1415 1420 1425 Leu Leu
Phe Gly Arg Arg Arg Lys Pro Lys Lys Asp 1430 1435 1440
94701DNAStaphylococcus pseudintermedius 9atgtttaatc aacaaaaaca
acactatggt atccggaaat atgcaatcgg gacttcatca 60gtattattag gcatgacatt
atttatcaca catgacgcaa ctgcatctgc agctgaaaac 120aatacaactg
caaagacaga gacaaatcaa gcagcaacaa tttcttctcg cacttcgcca
180accgacgtcg ctcaacctaa tgcagacacg aatgctacaa cggcgactaa
agagacaaca 240ccacaatcag attcaacagc attaccgcaa gcagcagcgc
aacctcaaac gggccaaaca 300gcatcgaaag acacagtaga tacaaataaa
acgcaaacag cagattccac aaccgctcct 360cctgtgacag acgcgccaaa
agctaatgac gacacaacac agccagaagc tgcgactgta 420gccaaaaaag
aagatgctca gacaccatcg actgcagacc ctacaccaca agcgcaacaa
480ccgcctcagt caaaagcacc tcaagaaacg caacaacaat caacagttga
agatacaacg 540ccacaacaaa acgcatcaac tgaagcacac cctaaaaatg
tagataccgc ttcaacaaaa 600caacaacaaa caacgccatc aaccgcaccg
acaccttaca cacaacaagc agacgaagca 660atgacagatg tcacaacaac
cagtgtcgac agcaacgtac agccgttagc ccctgcagaa 720gatcaaccta
aaaatacgaa cacagctgac aaagcaaccg ttgcgacacc accacgtgac
780aatgctaaga ctgctgatcc gaacaaaaag atgacacgtg cagcaacgac
acaacaagat 840gatgccgtcg atacattgaa gtcaaaagaa atgacagcaa
cgatcgataa aagttttcca 900gccgttaaat attacacgtt gaaaaatggt
aaaaaagtcg atgcacaact gacggatgca 960cgtcaaatca tcgtcaatgg
tgaagtcatt acaccaacag tcaaatacaa caaaattgat 1020gatcatacgg
ctgaatatga cttaacagca caaaatgatt cacgttcgat tgatgccaat
1080tttaaatttc gtttatcagt tgaaggtaag accgttgatt tacaaatgac
agattacacg 1140aacaacaaca cagatccaca aaacgtcatt cgcaacttta
gctttgtaag tcaatcgctc 1200gtatctgtaa acaatcaaca gaaaaatgcc
aaactgcaaa catcgaaact gtctacaaat 1260acaatgaaaa gcggcgataa
atcatatcat atcgatgaaa atttcaaaaa cgacttcaac 1320gactttatga
tgtacggttt cgtgtcaaat gatgattaca gtgcaggatt gtggagtaac
1380gcacaaattg gcgtcggcat tggtgaacaa gacttcttac gtgtctacgc
acagtctata 1440caaacagata tcggggtcgc tgtcggttta ggctcaatgc
catggtttat ccaaaaagac 1500gctgcacatc cagatgcgaa aaatcaagga
ctactcccac atgtcaaagt tgcaattgcg 1560gaagatgaaa atcaagatgg
tgaaattaac tggcaagacg gtgcaattgc ttatcgtagc 1620attatgaaca
atccatatgg tgccgaagaa gtacctgacc ttgttgggta ccgtatcgcg
1680atgaactttg gttctcaagc gcaaaaccca tttttaaaga cgttagatgg
tgtgaaaaaa 1740ttctatctca atacagatgg tttagggcaa tccattttat
taaaaggtta taacagtgaa 1800ggccacgact ctggtcattt agattacgcg
aatattggtc aacgtatagg tggcgtgaaa 1860gactttaaaa cgttacttca
aaaaggggca gattatggcg cacgtttcgg tcttcatgtg 1920aatgcatctg
aaacatatcc agagtctcaa gcattcaatc ctgccctctt acgtaaagat
1980gcgaatggaa actatatgta tggctggaac tggctcgatc aaggctttaa
catcgatgca 2040gattacgatt taatacacgg gcgtaaagaa cgcttcgaag
cactcaaaca aattgtcggt 2100gatgacctcg actttattta tgtcgatgta
tgggggaatg gacaatccgg cgacaataca 2160gcttggccat cacatcaatt
agccaaagaa atcaacgact taggatggcg cgtcggtgtc 2220gaatggggtc
acggtatgga atatgactcc acgttccaac attgggcagc cgacttaacg
2280tatggatcgt accaaaataa agggattaac tcagaggtag cacgcttctt
acgcaaccat 2340caaaaagatt catgggtcgg taactatcca aaatactcag
gtgcagctga cttcccattg 2400ctcggcggtt atgacatgaa agattttgaa
ggttggcaag gtcgtaacga ttactctgct 2460tacattaaaa atattttcaa
tgttgatgta ccaacaaagt ttttacaaca ttataaagtg 2520atgcgtattg
tcgatggtga gcctgttaaa atgactgcca atggtcaaac gattgactgg
2580acaccagaaa tgcaagtcga tttacaaaat gaagccggtg atcaagtcac
tgttaaacgt 2640aaatctaacg actatgaaaa cgacactgac aactaccgct
cacgtacaat cgaattgaat 2700ggtcgcacag tactcgatgg cgattcatac
cttttaccat ggaattggga tgcgaacggc 2760caaccattaa ctggcgataa
cgaaaaatta tatcactgga ataaaaaagg cggttcaacg 2820acttggacac
tgcctgaatc atgggataca gaccaagtcg tgctatacga attatctgaa
2880acgggtcgta agtcaccacg tacagtggca gtgaaagacc atcaagtgac
actcgataat 2940attaaagcag acacaccgta tgtcgtttat aaagtcgcac
aaccagacaa cacagatgtg 3000aactggagcg aagacatgca cgtgaaagat
gccggcttca actcacaaca actgacacct 3060tggacaatcg aaggcaatcg
agataaagtg agcatcgaaa agtcgacaac atcaaatgaa 3120atgctaaaaa
tcgatagtcc aacaaaaaca acgcaattaa cgcaacaatt gacaggttta
3180gtgccaggac aacgttacgc tgtctatgtt ggcatcgata accgcagtga
tgcagcggcg 3240catattgcag tgacacataa cggtaaaacg ctcgcaagta
acgaaacagg tcaatcgatc 3300gcgaaaaact atgtgaaagc agatgcacat
agtaacaatg ctgcgacgtt taaaaatggc 3360ggtagttact tccaaaacat
gtacgtgtac ttcgttgcgc cagaagatgg taaagcagac 3420ttgacgattc
aacgcgaccc aggtgaaggg gccacttatt tcgatgatat tcgtgtgtta
3480gaaaataacg cgaatctcct tcaaaacggc acattcaacc aagacttcga
aaatgtacca 3540caagggttat tcccgttcgt cgtgtcagaa gttgaaggcg
ttgaagataa tcgcgttcac 3600ttatctgaaa agcacgcacc gtatacacaa
cgcggatgga ataataaacg tgtcgatgat 3660gtcattgatg gcaaatggtc
acttaaagta aacggtcaaa caggtaaaga taaaatggtc 3720atccaaacga
ttccgcaaaa cttctacttc gaaccaggaa aaacgtatga agtgtcattt
3780gattatgaag caggttctga tgatacgtat gcatttgcga caggtagtgg
ggacatttct 3840aaaaatcgta actttgaaaa gacaccattg aaaaatacag
tcgatggtgg caaagcgaaa 3900cgggtgacat ttaaagtgac gggtgatgaa
aatggtcaaa cttggatcgg tatttactca 3960acgaaaacac ccaatgatcc
acgaggcgtg aaaaatggca atcaaatcaa cttcgaaggg 4020acgaaagatt
tcattctaga caacctttct atccgtgaaa ttgacgcacc gaagcctgat
4080gccacacaag aaagcggtga tagcgcacca atgaatgaaa cagatgagcg
taacgtcaat 4140tcaaacggta cattagccga tcatagtgag acaactgatg
tcaatgtcag tgcaacggca 4200gatgatacag cagtcaaagg cgaaatgacg
acaaacagaa cagatgcacc aactgttaca 4260ctgcctgaag caacgatagt
agatgaaggc acgtcaaatc ctgtcactac aacaccaacg 4320aatacaacac
aagctatgac aaataaggct gatgagatgc cacaaacgat gaacaatgtt
4380cctttaacta gcatcgctac cgatatgatg cagtctcatg cggtggattc
catggcagca 4440acactagcag ctacaaatca agtggcggca cctgtgcgtc
aaacagcagg acctatgcaa 4500catggtatgg acagtgcttc aacgcaacac
gcacccatac aagttgacaa tgtcacagca 4560ccaccattac cagatgaaca
gtttgccgaa ttacctaaaa ctggggatac gactccaaat 4620acacgtggac
ctttaatggc gatgatagtt ggcgcagtct taacagcatt cggattcaga
4680cgccaacgta aagaaaaata g 4701101566PRTStaphylococcus
pseudintermedius 10Met Phe Asn Gln Gln Lys Gln His Tyr Gly Ile Arg
Lys Tyr Ala Ile 1 5 10 15 Gly Thr Ser Ser Val Leu Leu Gly Met Thr
Leu Phe Ile Thr His Asp 20 25 30 Ala Thr Ala Ser Ala Ala Glu Asn
Asn Thr Thr Ala Lys Thr Glu Thr 35 40 45 Asn Gln Ala Ala Thr Ile
Ser Ser Arg Thr Ser Pro Thr Asp Val Ala 50 55 60 Gln Pro Asn Ala
Asp Thr Asn Ala Thr Thr Ala Thr Lys Glu Thr Thr 65 70 75 80 Pro Gln
Ser Asp Ser Thr Ala Leu Pro Gln Ala Ala Ala Gln Pro Gln 85 90 95
Thr Gly Gln Thr Ala Ser Lys Asp Thr Val Asp Thr Asn Lys Thr Gln 100
105 110 Thr Ala Asp Ser Thr Thr Ala Pro Pro Val Thr Asp Ala Pro Lys
Ala 115 120 125 Asn Asp Asp Thr Thr Gln Pro Glu Ala Ala Thr Val Ala
Lys Lys Glu 130 135 140 Asp Ala Gln Thr Pro Ser Thr Ala Asp Pro Thr
Pro Gln Ala Gln Gln 145 150 155 160 Pro Pro Gln Ser Lys Ala Pro Gln
Glu Thr Gln Gln Gln Ser Thr Val 165 170 175 Glu Asp Thr Thr Pro Gln
Gln Asn Ala Ser Thr Glu Ala His Pro Lys 180 185 190 Asn Val Asp Thr
Ala Ser Thr Lys Gln Gln Gln Thr Thr Pro Ser Thr 195 200 205 Ala Pro
Thr Pro Tyr Thr Gln Gln Ala Asp Glu Ala Met Thr Asp Val 210 215 220
Thr Thr Thr Ser Val Asp Ser Asn Val Gln Pro Leu Ala Pro Ala Glu 225
230 235 240 Asp Gln Pro Lys Asn Thr Asn Thr Ala Asp Lys Ala Thr Val
Ala Thr 245 250 255 Pro Pro Arg Asp Asn Ala Lys Thr Ala Asp Pro Asn
Lys Lys Met Thr 260 265 270 Arg Ala Ala Thr Thr Gln Gln Asp Asp Ala
Val Asp Thr Leu Lys Ser 275 280 285 Lys Glu Met Thr Ala Thr Ile Asp
Lys Ser Phe Pro Ala Val Lys Tyr 290 295 300 Tyr Thr Leu Lys Asn Gly
Lys Lys Val Asp Ala Gln Leu Thr Asp Ala 305 310 315 320 Arg Gln Ile
Ile Val Asn Gly Glu Val Ile Thr Pro Thr Val Lys Tyr 325 330 335 Asn
Lys Ile Asp Asp His Thr Ala Glu Tyr Asp Leu Thr Ala Gln Asn 340 345
350 Asp Ser Arg Ser Ile Asp Ala Asn Phe Lys Phe Arg Leu Ser Val Glu
355 360 365 Gly Lys Thr Val Asp Leu Gln Met Thr Asp Tyr Thr Asn Asn
Asn Thr 370 375 380 Asp Pro Gln Asn Val Ile Arg Asn Phe Ser Phe Val
Ser Gln Ser Leu 385 390 395 400 Val Ser Val Asn Asn Gln Gln Lys Asn
Ala Lys Leu Gln Thr Ser Lys 405 410 415 Leu Ser Thr Asn Thr Met Lys
Ser Gly Asp Lys Ser Tyr His Ile Asp 420 425 430 Glu Asn Phe Lys Asn
Asp Phe Asn Asp Phe Met Met Tyr Gly Phe Val 435 440 445 Ser Asn Asp
Asp Tyr Ser Ala Gly Leu Trp Ser Asn Ala Gln Ile Gly 450 455 460 Val
Gly Ile Gly Glu Gln Asp Phe Leu Arg Val Tyr Ala Gln Ser Ile 465 470
475 480 Gln Thr Asp Ile Gly Val Ala Val Gly Leu Gly Ser Met Pro Trp
Phe 485 490 495 Ile Gln Lys Asp Ala Ala His Pro Asp Ala Lys Asn Gln
Gly Leu Leu 500 505 510 Pro His Val Lys Val Ala Ile Ala Glu Asp Glu
Asn Gln Asp Gly Glu 515 520 525 Ile Asn Trp Gln Asp Gly Ala Ile Ala
Tyr Arg Ser Ile Met Asn Asn 530 535 540 Pro Tyr Gly Ala Glu Glu Val
Pro Asp Leu Val Gly Tyr Arg Ile Ala 545 550 555 560 Met Asn Phe Gly
Ser Gln Ala Gln Asn Pro Phe Leu Lys Thr Leu Asp 565 570 575 Gly Val
Lys Lys Phe Tyr Leu Asn Thr Asp Gly Leu Gly Gln Ser Ile 580 585 590
Leu Leu Lys Gly Tyr Asn Ser Glu Gly His Asp Ser Gly His Leu Asp 595
600 605 Tyr Ala Asn Ile Gly Gln Arg Ile Gly Gly Val Lys Asp Phe Lys
Thr 610 615 620 Leu Leu Gln Lys Gly Ala Asp Tyr Gly Ala Arg Phe Gly
Leu His Val 625 630 635 640 Asn Ala Ser Glu Thr Tyr Pro Glu Ser Gln
Ala Phe Asn Pro Ala Leu 645 650 655 Leu Arg Lys Asp Ala Asn Gly Asn
Tyr Met Tyr Gly Trp Asn Trp Leu 660 665 670 Asp Gln Gly Phe Asn Ile
Asp Ala Asp Tyr Asp Leu Ile His Gly Arg 675 680 685 Lys Glu Arg Phe
Glu Ala Leu Lys Gln Ile Val Gly Asp Asp Leu Asp 690 695 700 Phe Ile
Tyr Val Asp Val Trp Gly Asn Gly Gln Ser Gly Asp Asn Thr 705 710 715
720 Ala Trp Pro Ser His Gln Leu Ala Lys Glu Ile Asn Asp Leu Gly Trp
725 730 735 Arg Val Gly Val Glu Trp Gly His Gly Met Glu Tyr Asp Ser
Thr Phe 740 745 750 Gln His Trp Ala Ala Asp Leu Thr Tyr Gly Ser Tyr
Gln Asn Lys Gly 755 760 765 Ile Asn Ser Glu Val Ala Arg Phe Leu Arg
Asn His Gln Lys Asp Ser 770 775 780 Trp Val Gly Asn Tyr Pro Lys Tyr
Ser Gly Ala Ala Asp Phe Pro Leu 785 790 795 800 Leu Gly Gly Tyr Asp
Met Lys Asp Phe Glu Gly Trp Gln Gly Arg Asn 805 810 815 Asp Tyr Ser
Ala Tyr Ile Lys Asn Ile Phe Asn Val Asp Val Pro Thr 820 825 830 Lys
Phe Leu Gln His Tyr Lys Val Met Arg Ile Val Asp Gly Glu Pro 835 840
845 Val Lys Met Thr Ala Asn Gly Gln Thr Ile Asp Trp Thr Pro Glu Met
850 855 860 Gln Val Asp Leu Gln Asn Glu Ala Gly Asp Gln Val Thr Val
Lys Arg 865 870 875 880 Lys Ser Asn Asp Tyr Glu Asn Asp Thr Asp Asn
Tyr Arg Ser Arg Thr 885 890 895 Ile Glu Leu Asn Gly Arg Thr Val Leu
Asp Gly Asp Ser Tyr Leu Leu 900 905 910 Pro Trp Asn Trp Asp Ala Asn
Gly Gln Pro Leu Thr Gly Asp Asn Glu 915 920 925 Lys Leu Tyr His Trp
Asn Lys Lys Gly Gly Ser Thr Thr Trp Thr Leu 930 935 940 Pro Glu Ser
Trp Asp Thr Asp Gln Val Val Leu Tyr Glu Leu Ser Glu 945 950 955 960
Thr Gly Arg Lys Ser Pro Arg Thr Val Ala Val Lys Asp His Gln Val 965
970 975 Thr Leu Asp Asn Ile Lys Ala Asp Thr Pro Tyr Val Val Tyr Lys
Val 980 985 990 Ala Gln Pro Asp Asn Thr Asp Val Asn Trp Ser Glu Asp
Met His Val 995 1000 1005 Lys Asp Ala Gly Phe Asn Ser Gln Gln Leu
Thr Pro Trp Thr Ile 1010 1015 1020 Glu Gly Asn Arg Asp Lys Val Ser
Ile Glu Lys Ser Thr Thr Ser 1025 1030 1035 Asn Glu Met Leu Lys Ile
Asp Ser Pro Thr Lys Thr Thr Gln Leu 1040 1045 1050 Thr Gln Gln Leu
Thr Gly Leu Val Pro Gly Gln Arg Tyr Ala Val 1055 1060 1065 Tyr Val
Gly Ile Asp Asn Arg Ser Asp Ala Ala Ala His Ile Ala 1070 1075 1080
Val Thr His Asn Gly Lys Thr Leu Ala Ser Asn Glu Thr Gly Gln 1085
1090 1095 Ser Ile Ala Lys Asn Tyr Val Lys Ala Asp Ala His Ser Asn
Asn 1100 1105 1110 Ala Ala Thr Phe Lys Asn Gly Gly Ser Tyr Phe Gln
Asn Met Tyr 1115 1120 1125 Val Tyr Phe Val Ala Pro Glu Asp Gly Lys
Ala Asp Leu Thr Ile 1130 1135 1140 Gln Arg Asp Pro Gly Glu Gly Ala
Thr Tyr Phe Asp Asp Ile Arg 1145 1150 1155 Val Leu Glu Asn Asn Ala
Asn Leu Leu Gln Asn Gly Thr Phe Asn 1160 1165 1170 Gln Asp Phe Glu
Asn Val Pro Gln Gly Leu Phe Pro Phe Val Val 1175 1180 1185 Ser Glu
Val Glu Gly Val Glu Asp Asn Arg Val His Leu Ser Glu 1190 1195 1200
Lys His Ala Pro Tyr Thr Gln Arg Gly Trp Asn Asn Lys Arg Val 1205
1210 1215 Asp Asp Val Ile Asp Gly Lys Trp Ser Leu Lys Val Asn Gly
Gln 1220 1225 1230 Thr Gly Lys Asp Lys Met Val Ile Gln Thr Ile Pro
Gln Asn Phe 1235 1240 1245 Tyr Phe Glu Pro Gly Lys Thr Tyr Glu Val
Ser Phe Asp Tyr Glu 1250 1255 1260 Ala Gly Ser Asp Asp Thr Tyr Ala
Phe Ala Thr Gly Ser Gly Asp 1265 1270 1275 Ile Ser Lys Asn Arg Asn
Phe Glu Lys Thr Pro Leu Lys Asn Thr 1280 1285 1290 Val Asp Gly Gly
Lys Ala Lys Arg Val Thr Phe Lys Val Thr Gly 1295 1300 1305 Asp Glu
Asn Gly Gln Thr Trp Ile Gly Ile Tyr Ser Thr Lys Thr 1310 1315 1320
Pro Asn Asp Pro Arg Gly Val Lys Asn Gly Asn Gln Ile Asn Phe 1325
1330 1335 Glu Gly Thr Lys Asp Phe Ile Leu Asp Asn Leu Ser Ile Arg
Glu 1340 1345 1350 Ile Asp Ala Pro Lys Pro Asp Ala Thr Gln Glu Ser
Gly Asp Ser 1355 1360 1365 Ala Pro Met Asn Glu Thr Asp Glu Arg Asn
Val Asn Ser Asn Gly 1370 1375 1380 Thr Leu Ala Asp His Ser Glu Thr
Thr Asp Val Asn Val Ser Ala 1385 1390 1395 Thr Ala Asp Asp Thr Ala
Val Lys Gly Glu Met Thr Thr Asn Arg 1400 1405 1410 Thr Asp Ala Pro
Thr Val Thr Leu Pro Glu Ala Thr Ile Val Asp 1415 1420
1425 Glu Gly Thr Ser Asn Pro Val Thr Thr Thr Pro Thr Asn Thr Thr
1430 1435 1440 Gln Ala Met Thr Asn Lys Ala Asp Glu Met Pro Gln Thr
Met Asn 1445 1450 1455 Asn Val Pro Leu Thr Ser Ile Ala Thr Asp Met
Met Gln Ser His 1460 1465 1470 Ala Val Asp Ser Met Ala Ala Thr Leu
Ala Ala Thr Asn Gln Val 1475 1480 1485 Ala Ala Pro Val Arg Gln Thr
Ala Gly Pro Met Gln His Gly Met 1490 1495 1500 Asp Ser Ala Ser Thr
Gln His Ala Pro Ile Gln Val Asp Asn Val 1505 1510 1515 Thr Ala Pro
Pro Leu Pro Asp Glu Gln Phe Ala Glu Leu Pro Lys 1520 1525 1530 Thr
Gly Asp Thr Thr Pro Asn Thr Arg Gly Pro Leu Met Ala Met 1535 1540
1545 Ile Val Gly Ala Val Leu Thr Ala Phe Gly Phe Arg Arg Gln Arg
1550 1555 1560 Lys Glu Lys 1565 1110509DNAStaphylococcus
pseudintermedius 11atgacaagaa aatttaggga atttaagaaa agtttaagtg
aagaaaaagc aagagtgaaa 60ctttacaagt caggtaaaaa ctgggttaaa gctggaatta
aagaatttca gttattaaaa 120gcattaggct tatctttttt aagccatgac
attgtaaagg atgaaaatgg agaagtaacg 180acacaatttg gggaacagtt
gaagaaaaat gcattaagaa caactgcttt tgcgggtgga 240atgttcacag
ttaatatgtt gcatgaccaa caagcatttg cggcgtcgga tgcacctata
300acttctgaac tggcaaccaa aagtcaaact attggcgatc aaacatcaat
tgttattgaa 360aagtctacat cgtcagatca atcaacgaac ccaataacag
aaagtgaaag taaacacgat 420tctgaaagta tctcattatc tgagcatcaa
acatcagagt caacaagtct ttcaacgtca 480acttccaaat caatatcaac
ttcagtagag gaatcagaat caacatcaaa agattctcat 540actaaaactc
aagatagtca atcagatagt catcagtcaa caagtcaaga ggtaaatggc
600tcttccaacc acgagcaatc aacaccacac actgcacaaa gtcttacgag
cctatctatt 660gagagccaaa cgtcgacttc aaatacatca ttgaaggaaa
ctaaagaagg ggaattgtca 720aaaaaccttt cgaagttatc tcaaaatcaa
aacatcaaac ttcatgaaga acatacgatg 780cgttcagcag atttgagctc
aggttataca ggatttagag cggcttacta tgtaccaaga 840tcaagaacaa
caccaacgac aaaagtctac acagggcaag gaagcttcag aggtagaggt
900agaattaaat ataatatttt ctacaaagtt gtcgttacaa gtaatggcaa
agaaatgaag 960atccgctata cattgagtca agatgatcca aacacgtcta
atgttgaaaa acctaggtgg 1020gcaggacaga aacgatttgg tattcataat
acttgggatg aaggtcctgg tcgcgggcaa 1080ttaaagttag ggtcggcatt
cggcaaacca acagttatac aaggagaaac tagaccgaat 1140tatggtagct
gggttggcac acctataacg aaatatgttt caggcgatcg tacaaatggt
1200ttttactggc aagctgctgt acttgcaccg agacatggag agaagggaga
aggaatcaca 1260gcagaaatta cagttcctat tgttaaccct tctggaagat
ttaattggga attccatcct 1320gtcggtcaac aggacggagt tggtggcaaa
actgactact ttgaaaatgt atggattcga 1380gactatgacc catattacaa
atatattcaa actaaggaag gcagagcctc agtttcgcac 1440tctatttctc
aggtgaaagc aagtgaatcg agatcgacat cgctcataca atcggagtct
1500attagaagat cacagtccat atctgagagt gaatctattg tagccgcaag
tcattcggca 1560agtgtagcaa aatcgcaatc catctcgaga agtcaatctg
tggcgaaatc acaatcgatc 1620tcaagaagtc agtcgatcgc acacagccga
tcagcaagtg tggcaaaatc gcaatccatc 1680tcaagaagtc agtcgatcgc
acacagccga tcagcaagtg tggcaaaatc acaatcgatt 1740tcaagaagtc
agtcgatcgc acacagccga tcagcaagtg tggcgaaatc tcaatcgatt
1800tcaagaagtc agtcaattgc gcagagccaa tcagcaagtg tggcaaaatc
acagtcgatt 1860tcaagaagtc agtcaattgc gcagagccaa tcagcaagtg
tggcgaaatc gcaatcgatt 1920tcaagaagtc agtcgattgc acatagccga
tcagcaagtg tagcggaatc acagtcgatt 1980tcaagaagtc agtcgattgc
gaatagccaa tctgtagcag cgagtgaatc agagagtcta 2040tcaatatcat
tgtctaaaaa gcagtcaata tcgatgagta attctgaaag tgcagcaaaa
2100tcacactcgc tttcggtgaa aaggtctaac tggattaaaa agtcaaaagc
ggcttcagta 2160agaaagtcac attcactttc ggtaagaaaa tctaattcgg
cgaaaaggtc acatgctatt 2220tcggtaagaa agtcaaagtc attatcagtt
aaaaagtcaa tttcgcagag ccaatcagca 2280agtgtggcga aatcgcaatc
gatttcaaga agtcaatcag tagcagcgag tgagtcggca 2340tcgctaagta
agtcgaagag cacatcgctc agtaactcag tgagtgcaga gaaatcgacg
2400tcattaagtc gttcagcaag tgtagcaaaa tcgcaatcga tttcaagaag
ccaatcagta 2460gtagcgagcg aatcggcatc gttaagtaag tcgaagagca
catcgctcag taactcagtg 2520agtgcagaga aatcgacgtc attaagtcga
tcagcaagtg tagcaaaatc gcaatcgatt 2580tcaagaagcc aatcggtggc
agcgagcgaa tcggcatcgt taagtaagtc gaagagcaca 2640tcgctcagta
actcagtgag tgcagagaaa tcgacgtcat taagtcgatc agcaagtgta
2700gcaaaatcgc aatcgatttc aagaagccaa tcggtggcag cgagcgaatc
ggcatcgtta 2760agtaagtcga agagcacatc gctcagtaac tcagtgagtg
cagagaaatc gacgtcatta 2820agtcgatcag caagtgtggc aaaatcgcaa
tcgatttcaa gaagccaatc agtagtagcg 2880agcgaatcgg catcgttaag
taagtcgaag agcacatcgc tcagtaactc agtgagtgca 2940gagaaatcga
cgtcattaag tcgatcagca agtgtagcaa aatcgcaatc gatttcaaga
3000agccaatcgg tggcagcgag cgaatcggca tcgttaagta agtcgaagag
cacatcgctc 3060agtaactcag tgagtgcaga gaaatcgacg tcattaagtc
gatcagcaag tgtggcaaaa 3120tcgcaatcga tttcaagaag ccagtcagta
gcagcaagtg agtcggcatc attaagtaag 3180tcgaagagca catctttaag
caactcagtg agtgtagaga aatcgacgtc attaagtcga 3240tcagcaagtg
tggcgaaatc gcaatcgatt tcaagaagtc aatcagtagc agcgagtgag
3300tcggcatcgc taagtaagtc gaagagcaca tcgctcagta actcagtgag
tgcagagaaa 3360tcgacgtcat taagtcgttc agcaagtgta gcaaaatcgc
aatcgatttc aagaagccag 3420tcagtagcag caagtgagtc ggcatcattg
agtaaatcaa caagtacgtc aacaagtgac 3480tcagatagcg cgtcaacatc
aacatctgta tcagatagcg attcagcttc attgagtaag 3540tcgactagta
catcaacaag cgattcagac agcgcgtcag catcattgag caagtcaaca
3600agtacatcaa cgagcgactc agatagcgca tcgacatcaa catcagtatc
agatagcgac 3660tccgcatcgt tgagtaaatc gacaagcacg tcaacaagtg
attcagacag cacgtctact 3720tcattgagta agtcgacaag tacatcgaca
agtgattcag atagtgcgtc aaaatcaacg 3780tcagtatcag acagtacgtc
cgcatcattg agtaaatcga caagcacgtc aacaagtgat 3840tcagatagtg
catcaaaatc aacgtcggta tcagatagca cgtcagcatc attaagaaag
3900tcggcaagta cgtcaacgag tgactcagac agcacgtcta cttcattgag
taagtcgaca 3960agtacatcga caagtgattc agatagtgca tcaaaatcaa
catcagtatc agatagcgat 4020tcagcttcat tgagtaagtc gactagtaca
tcaacaagcg attcagatag tgcgtcaaaa 4080tcaacgtcgg tatcagatag
cgactccgca tcgttgagta agtcgacaag tacgtcaaca 4140agcgattcag
acagtgcatc aaaatcaacg tcggtatcag acagtacgtc aacatcatta
4200agtaagtcga caagtacatc aacaagcgat tcagatagtg cgtcaacatc
gacatcagta 4260tcggacagta cgtctgcatc attgagtaag tcgacaagca
catcgacaag tgattcagat 4320agcgcatcaa catcagtgtc agatagcgat
tcagcatcac taagcaagtc aacaagtaca 4380tcgacaagcg attcagacag
cgtatcaaca tcaacatcag tatcagatag tgattccgcg 4440tcattaagta
agtcgacaag tacgtcaaca agcgattcag atagtgcgtc aaaatcaaca
4500tcagtatcag atagcacgtc aacatcattg agtaaatcaa caagtacatc
gacaagtgac 4560tcagatagtg cgtcaacatc ggtatcagac agtacgtccg
catcattgag taaatcgaca 4620agcacgtcaa caagtgattc agatagtgca
tcaaaatcaa catcagtatc agatagcgat 4680tcagcatcat taagcaagtc
gacaagtaca tcgacaagtg attcagatag tgcgtcaaca 4740tcaacgtcag
tgtcagatag cgattcagct tcattaagca aatcaacaag tacgtcaaca
4800agtgactcag atagcgcatc aacatcatta agcaagtcaa caagtacatc
gacaagcgat 4860tcagacagta cgtctacatc attaagtaag tcaacaagta
catcaacaag tgattcggat 4920agtgcgtcaa aatcaacatc agtatcagat
agcgactcag cttcattaag caagtcgaca 4980agtacgtcaa caagtgactc
agacagtgcg tcaaaatcaa catctgtgtc agatagcgac 5040tccgcatcgt
tgagtaagtc gacaagtacg tcaacgagcg attcggatag tgcgtcaaaa
5100tcaacatcag tatcagatag tgaatccgcg tcattaagca agtcgacaag
cacatcgaca 5160agtgactcag atagtgcgtc aacatcgaca tcggtatcag
acagcacatc agtttcatta 5220agcaagtcga caagcacgtc aacaagcgat
tcagacagta cgtctacttc attaagcaag 5280tcgacaagca cgtcaacaag
tgactcagat agtgactcag cttcgttgag taaatcgaca 5340agcacgtcaa
cgagcgattc agatagcgtg tcaacatcaa catctgtgtc agatagcgat
5400tcagcttcat taagcaaatc gacaagtaca tcaacaagcg attcagatag
tgcgtcaaca 5460tcaacgtcgg tatcagatag cggctccgca tcgttgagta
agtcgacaag tacgtcaacg 5520agcgattcag acagtgcatc aaaatcaacg
tcggtatcag atagtgattc agcatcacta 5580agcaaatcga caagcacgtc
aacaagtgac tcagacagtg cgtcaacatc gacatcggta 5640tcagatagca
catccgcgtc gttaagcaag tcgacaagta cgtcaacaag tgattcagac
5700agcgcatcga catcaacatc agtatcagat agcgactccg catcgttgag
taaatcgaca 5760agcacgtcaa caagtgattc ggacagtgcg tcaaaatcaa
catcagtgtc agatagcgat 5820tcagcttcat tgagtaagtc gacaagcacg
tcaacaagcg aatcagacag cgcgtcaaaa 5880tcaacgtcag tgtcagatag
cgattccgca tcattaagta aatcgacaag cacgtcaaca 5940agtgactcag
atagtgcatc gacatcaacg tcagtatcag atagtgattc cgcgtcatta
6000agcaagtcga caagtacgtc aacaagtgac tcagacagtg cgtcaaaatc
aacatcagta 6060tcagatagcg attccgcatc attgagtaag tcgacaagca
cgtcaacaag cgaatcagac 6120agtgcgtcaa catcgacatt agtatcggat
agtacgtcgg tttcattgag ccaatcaaca 6180agtgtggata aagatagtac
agcgaaggga tcgacagaat tagtaaatgt tgcatcactt 6240tcaatcagtg
cgagtcaatc aagtagttta tctgcttcaa catccacatc gattgaaaag
6300tctgagtcta catcaacaag tggctcaaat tcaactaatg cgtcgttaag
tagctcatct 6360tcacttagta catcagcaag tacttctgta agcgaagtga
catctgtcac acattctgaa 6420aatgatttaa gtgcatctaa cgatagagat
acatccggat cagtaagtca atttgcttct 6480gaaaatacat cattaagtga
ttctgcatca attagtggcg aagtttctag tagtacgtcc 6540gcgtcaactt
cgaaatcatc atcactttca gcaagcgcgt tacatgataa gcatgtatca
6600gaaagcactt ctgcatcatt aagtagtgga gattcaagtc gtgcttcggc
atcagtgtca 6660acgtcattat cagaatcaga tagtgcgtta atagactctg
aatcaattag cgtttccgag 6720cacacatcaa cattacaatc aggtagtcat
tcactatcac aacaacaatc agcagaatta 6780tcacaatcag agcaaacatc
acaatcacaa cgcatttcaa caagtgcgtc agtatcggct 6840atgaaatcag
aaagtgctgc taaggtatct gaatcgctat ctacgtctca atcaaaagta
6900gatagtcaat cacaatcggt atctgaatca gcgagcaact cacgagtgtc
aagagattca 6960aaatcaacaa gcgcttcaat gcatcgatca ttgtcagagt
cagtatctca aagtatgtca 7020cttattgatc agtcagaaag tgattcaaca
tctatatcga tttcgacgtc aatcagtgat 7080gaagactcta tgctgtattc
tatgagtgat tccgcatcga tcagtactaa ggcatcaagt 7140agtatgtcta
cttcgacaag cgaagagcat gccaacagtc attctcagtc tgaatcgaca
7200gcatcggttg aagtatctca agaaatgagt gcatcggctt caacaagcaa
atctgagtct 7260caatcagagt cagtatcagt aagtaacgaa gaatcaaata
tctcatctat gcaagagtct 7320tttgtagaga gtgcaaaagc atcgcgtagt
gcatctatga gcgttgcaaa atctgaagcc 7380tctgaatcac agctattaag
tgagtctaat gcttcggtaa gccaatcagc aagcacaagt 7440agtaaagcat
cagcaagtac gtcagaatct atttcaacgt cactcagcgt atctgaagca
7500actcatggaa aaccgagaaa tcattcagaa agtgcatcag caagtcaatt
attagaagaa 7560aatgagtcat taagcgattc agcatcaaca agtgttgaag
attcagaaag tgcatcagca 7620tctctgtcgg tgtatcaatc acaatcagca
agtgcattga aatcaacaca tgcatcagaa 7680aaagcttcag tgaatacaag
tgcaaacgca tcgaagcgtg catcagcatc gacatctatc 7740tctaactcga
aatctaaagt cattgcgagt gaatcgaagt caacaagcat atcaacatat
7800gaatcgttgt caatatcgac tagtaaagaa caatcaacgc gtgtatcagt
gagtgagtcg 7860acatcaacgt ctaaagtgaa gtcagaaagc gactcggcat
caacgtcgac atctgaatca 7920atctcaatta gcgcaaatcg ttcaggttac
acatcgtcta aacgttcggt acaaatgagt 7980gaagcacaat caacgagcga
ttcattatca gtaatgcaat ctgaaggttc agtaagtgta 8040tcgcaatctt
taagtatatc agataagaca tcacagtcct tatcggaatc aatatcgcat
8100tcagaaagtg actctgatag taactcagtg tctattagtc aagagacatc
tgaacaacat 8160tcggtgtcag acagtgactc gatgtcaatt tcggaaagcg
aatctattgc atatagtcaa 8220tcagcgagtg aatcagaatc aacaagtatc
gcaaaatctg atagtatttc gaactcatta 8280tctgtttcat taagtgaatc
agaaagtgaa gcaagcacat cagcttcagt gagtacatct 8340gaaagtacgt
ctgtaaaggg ttctctatca acaagtatct tgaacagtca atcagcatct
8400actcatcaat caacagaagc ttctcaaagt acatcaactt caaaagttga
ggaagcatca 8460ttgagtgact ctgcttctgt atcagattca caatcacttt
caatgagtca tgagaaatca 8520caaagtgcat cgacttcaaa atctacgagt
ctgtcaaaaa ctatttctga gtcagagtct 8580gtgagtgcat caacatcaac
aagtgaagct gtaagtacag aagcaagcga atttgtatca 8640gcagtagact
cattgagtca agtaacttct aacggaagca caacgaaaga agatgcgagt
8700acatttgtat ccacagtaga ttcattgaaa gacaaagcat caaataatgg
tacaccatca 8760gagtttgcgt cagcagtgaa atcaacacac gcatcagtga
gtgtgtcagc atcagaaagt 8820acgtcagcat caacatcaac aagtgaagct
gtaagtacag aagcaagcga atttgtatca 8880gcagtgaatt cgttgagtga
agcgacttct aacggaagca caacgaaaga agatgcgagt 8940acatttgtat
ccacagtaga ttcattgaaa gacaaagcat caaataatgg tacaccatca
9000gagtttgcgt cagcagtgaa atcaacacac gcatcagtga gtgtgtcagc
atcagaaagt 9060acatcagcat caacatcaac aagtgaagct gtaagtacag
aagcaagcga atttgtatca 9120gcagtagact cattgagtca agtaacttct
aacggaagca caacgaaaga agatgcaagc 9180acatttgtat ccacagtaga
ttcattgaaa gataaagcat caaacaatgg tacaccatca 9240gaatttgaat
cagttgtgaa atcagtacac ggatcaatga gtgcatcagc aagtgcgtca
9300acatcagcat ctacatcagc atctacatct acaagtgaag ctgcaagtgc
agaagcaagc 9360gaattagaat cagtaaggaa atcattatcc aatggagcat
caaacggtag cacagcaaga 9420gaaggtgcaa gcacatttgt atcaacggta
gattcattga aagataaagc atcaaacaat 9480ggtacagcat cagaatttga
atcagttgtg aagtcagtac acggatcaac aagtgcatca 9540gcaagtgcgt
caacgtcagc atcaacatca gcaagtgaat cagcaagtac agaagcaagt
9600gaatttgtat cagcagtggc atcattaagc agttcagcat ggaacggaag
cactacagga 9660gaaggtgcaa gcacatttgt atcaacagtt gattcatcga
aagattcagc gtcagacaaa 9720gcttcaccat cagaatcaga atcagttgtg
aagtcagtac acggatcaac gagtacatca 9780gcaagtgtgt cagcgtcggc
aagtacatca gcatcgacat caacaagtga agctgtaagc 9840acagaagcaa
gtgagtttgt atcagcagtg aactcattaa gcagtgaagc atcgaacggc
9900agcacaacaa gagaaggtgc aagcacattt gtatcaacag tagattcatt
gaaagacaaa 9960gcatcaaaca atggtacagc atcagaattt gaatcagttg
tgaagtcagt acacggatca 10020atgagtacat cagcaagtgt gtcagcatca
gaaagtacgt cggcatcgac atcgacaagt 10080gaagctgtaa gtacagaagc
aagcgagtca gcatcgataa gtgtatcaat gtcagtgagc 10140gcatcaacaa
gtgcttcaat gagcgtatca gtgtcaaaca gtgtgtcagt gagtgactct
10200atttcagtaa gtgcatcaac aagtgaacct aactcggtaa gcacttctat
gagtagttct 10260ctttcaacat cggcatcaac gccatcagaa attacttcaa
gttcgtcatc aagcgattca 10320gcgacagttc aaaaagtagt ttctaaagat
gaacagcacg ctacaaataa agttgaaaaa 10380ttacctgaca caggtcaatc
aacgacacaa actggtttat tgggtggagt aggtgcttta 10440cttacaggcc
ttggtttact caaaaaatca agaaaacaaa aagatgaaga aacatcatca
10500catgaataa 10509123502PRTStaphylococcus pseudintermedius 12Met
Thr Arg Lys Phe Arg Glu Phe Lys Lys Ser Leu Ser Glu Glu Lys 1 5 10
15 Ala Arg Val Lys Leu Tyr Lys Ser Gly Lys Asn Trp Val Lys Ala Gly
20 25 30 Ile Lys Glu Phe Gln Leu Leu Lys Ala Leu Gly Leu Ser Phe
Leu Ser 35 40 45 His Asp Ile Val Lys Asp Glu Asn Gly Glu Val Thr
Thr Gln Phe Gly 50 55 60 Glu Gln Leu Lys Lys Asn Ala Leu Arg Thr
Thr Ala Phe Ala Gly Gly 65 70 75 80 Met Phe Thr Val Asn Met Leu His
Asp Gln Gln Ala Phe Ala Ala Ser 85 90 95 Asp Ala Pro Ile Thr Ser
Glu Leu Ala Thr Lys Ser Gln Thr Ile Gly 100 105 110 Asp Gln Thr Ser
Ile Val Ile Glu Lys Ser Thr Ser Ser Asp Gln Ser 115 120 125 Thr Asn
Pro Ile Thr Glu Ser Glu Ser Lys His Asp Ser Glu Ser Ile 130 135 140
Ser Leu Ser Glu His Gln Thr Ser Glu Ser Thr Ser Leu Ser Thr Ser 145
150 155 160 Thr Ser Lys Ser Ile Ser Thr Ser Val Glu Glu Ser Glu Ser
Thr Ser 165 170 175 Lys Asp Ser His Thr Lys Thr Gln Asp Ser Gln Ser
Asp Ser His Gln 180 185 190 Ser Thr Ser Gln Glu Val Asn Gly Ser Ser
Asn His Glu Gln Ser Thr 195 200 205 Pro His Thr Ala Gln Ser Leu Thr
Ser Leu Ser Ile Glu Ser Gln Thr 210 215 220 Ser Thr Ser Asn Thr Ser
Leu Lys Glu Thr Lys Glu Gly Glu Leu Ser 225 230 235 240 Lys Asn Leu
Ser Lys Leu Ser Gln Asn Gln Asn Ile Lys Leu His Glu 245 250 255 Glu
His Thr Met Arg Ser Ala Asp Leu Ser Ser Gly Tyr Thr Gly Phe 260 265
270 Arg Ala Ala Tyr Tyr Val Pro Arg Ser Arg Thr Thr Pro Thr Thr Lys
275 280 285 Val Tyr Thr Gly Gln Gly Ser Phe Arg Gly Arg Gly Arg Ile
Lys Tyr 290 295 300 Asn Ile Phe Tyr Lys Val Val Val Thr Ser Asn Gly
Lys Glu Met Lys 305 310 315 320 Ile Arg Tyr Thr Leu Ser Gln Asp Asp
Pro Asn Thr Ser Asn Val Glu 325 330 335 Lys Pro Arg Trp Ala Gly Gln
Lys Arg Phe Gly Ile His Asn Thr Trp 340 345 350 Asp Glu Gly Pro Gly
Arg Gly Gln Leu Lys Leu Gly Ser Ala Phe Gly 355 360 365 Lys Pro Thr
Val Ile Gln Gly Glu Thr Arg Pro Asn Tyr Gly Ser Trp 370 375 380 Val
Gly Thr Pro Ile Thr Lys Tyr Val Ser Gly Asp Arg Thr Asn Gly 385 390
395 400 Phe Tyr Trp Gln Ala Ala Val Leu Ala Pro Arg His Gly Glu Lys
Gly 405 410 415 Glu Gly Ile Thr Ala Glu Ile Thr Val Pro Ile Val Asn
Pro Ser Gly 420 425 430 Arg Phe Asn Trp Glu Phe His Pro Val Gly Gln
Gln Asp Gly Val Gly 435 440 445 Gly Lys Thr Asp Tyr Phe Glu Asn Val
Trp Ile Arg Asp Tyr Asp Pro 450 455 460 Tyr Tyr Lys Tyr Ile Gln Thr
Lys Glu Gly Arg Ala Ser Val Ser His 465 470 475 480 Ser Ile Ser Gln
Val Lys Ala Ser Glu Ser Arg Ser Thr Ser Leu Ile 485 490 495 Gln Ser
Glu Ser Ile Arg Arg Ser Gln Ser Ile Ser Glu Ser Glu Ser 500 505 510
Ile Val Ala Ala Ser His Ser Ala Ser Val Ala Lys Ser Gln Ser Ile 515
520 525
Ser Arg Ser Gln Ser Val Ala Lys Ser Gln Ser Ile Ser Arg Ser Gln 530
535 540 Ser Ile Ala His Ser Arg Ser Ala Ser Val Ala Lys Ser Gln Ser
Ile 545 550 555 560 Ser Arg Ser Gln Ser Ile Ala His Ser Arg Ser Ala
Ser Val Ala Lys 565 570 575 Ser Gln Ser Ile Ser Arg Ser Gln Ser Ile
Ala His Ser Arg Ser Ala 580 585 590 Ser Val Ala Lys Ser Gln Ser Ile
Ser Arg Ser Gln Ser Ile Ala Gln 595 600 605 Ser Gln Ser Ala Ser Val
Ala Lys Ser Gln Ser Ile Ser Arg Ser Gln 610 615 620 Ser Ile Ala Gln
Ser Gln Ser Ala Ser Val Ala Lys Ser Gln Ser Ile 625 630 635 640 Ser
Arg Ser Gln Ser Ile Ala His Ser Arg Ser Ala Ser Val Ala Glu 645 650
655 Ser Gln Ser Ile Ser Arg Ser Gln Ser Ile Ala Asn Ser Gln Ser Val
660 665 670 Ala Ala Ser Glu Ser Glu Ser Leu Ser Ile Ser Leu Ser Lys
Lys Gln 675 680 685 Ser Ile Ser Met Ser Asn Ser Glu Ser Ala Ala Lys
Ser His Ser Leu 690 695 700 Ser Val Lys Arg Ser Asn Trp Ile Lys Lys
Ser Lys Ala Ala Ser Val 705 710 715 720 Arg Lys Ser His Ser Leu Ser
Val Arg Lys Ser Asn Ser Ala Lys Arg 725 730 735 Ser His Ala Ile Ser
Val Arg Lys Ser Lys Ser Leu Ser Val Lys Lys 740 745 750 Ser Ile Ser
Gln Ser Gln Ser Ala Ser Val Ala Lys Ser Gln Ser Ile 755 760 765 Ser
Arg Ser Gln Ser Val Ala Ala Ser Glu Ser Ala Ser Leu Ser Lys 770 775
780 Ser Lys Ser Thr Ser Leu Ser Asn Ser Val Ser Ala Glu Lys Ser Thr
785 790 795 800 Ser Leu Ser Arg Ser Ala Ser Val Ala Lys Ser Gln Ser
Ile Ser Arg 805 810 815 Ser Gln Ser Val Val Ala Ser Glu Ser Ala Ser
Leu Ser Lys Ser Lys 820 825 830 Ser Thr Ser Leu Ser Asn Ser Val Ser
Ala Glu Lys Ser Thr Ser Leu 835 840 845 Ser Arg Ser Ala Ser Val Ala
Lys Ser Gln Ser Ile Ser Arg Ser Gln 850 855 860 Ser Val Ala Ala Ser
Glu Ser Ala Ser Leu Ser Lys Ser Lys Ser Thr 865 870 875 880 Ser Leu
Ser Asn Ser Val Ser Ala Glu Lys Ser Thr Ser Leu Ser Arg 885 890 895
Ser Ala Ser Val Ala Lys Ser Gln Ser Ile Ser Arg Ser Gln Ser Val 900
905 910 Ala Ala Ser Glu Ser Ala Ser Leu Ser Lys Ser Lys Ser Thr Ser
Leu 915 920 925 Ser Asn Ser Val Ser Ala Glu Lys Ser Thr Ser Leu Ser
Arg Ser Ala 930 935 940 Ser Val Ala Lys Ser Gln Ser Ile Ser Arg Ser
Gln Ser Val Val Ala 945 950 955 960 Ser Glu Ser Ala Ser Leu Ser Lys
Ser Lys Ser Thr Ser Leu Ser Asn 965 970 975 Ser Val Ser Ala Glu Lys
Ser Thr Ser Leu Ser Arg Ser Ala Ser Val 980 985 990 Ala Lys Ser Gln
Ser Ile Ser Arg Ser Gln Ser Val Ala Ala Ser Glu 995 1000 1005 Ser
Ala Ser Leu Ser Lys Ser Lys Ser Thr Ser Leu Ser Asn Ser 1010 1015
1020 Val Ser Ala Glu Lys Ser Thr Ser Leu Ser Arg Ser Ala Ser Val
1025 1030 1035 Ala Lys Ser Gln Ser Ile Ser Arg Ser Gln Ser Val Ala
Ala Ser 1040 1045 1050 Glu Ser Ala Ser Leu Ser Lys Ser Lys Ser Thr
Ser Leu Ser Asn 1055 1060 1065 Ser Val Ser Val Glu Lys Ser Thr Ser
Leu Ser Arg Ser Ala Ser 1070 1075 1080 Val Ala Lys Ser Gln Ser Ile
Ser Arg Ser Gln Ser Val Ala Ala 1085 1090 1095 Ser Glu Ser Ala Ser
Leu Ser Lys Ser Lys Ser Thr Ser Leu Ser 1100 1105 1110 Asn Ser Val
Ser Ala Glu Lys Ser Thr Ser Leu Ser Arg Ser Ala 1115 1120 1125 Ser
Val Ala Lys Ser Gln Ser Ile Ser Arg Ser Gln Ser Val Ala 1130 1135
1140 Ala Ser Glu Ser Ala Ser Leu Ser Lys Ser Thr Ser Thr Ser Thr
1145 1150 1155 Ser Asp Ser Asp Ser Ala Ser Thr Ser Thr Ser Val Ser
Asp Ser 1160 1165 1170 Asp Ser Ala Ser Leu Ser Lys Ser Thr Ser Thr
Ser Thr Ser Asp 1175 1180 1185 Ser Asp Ser Ala Ser Ala Ser Leu Ser
Lys Ser Thr Ser Thr Ser 1190 1195 1200 Thr Ser Asp Ser Asp Ser Ala
Ser Thr Ser Thr Ser Val Ser Asp 1205 1210 1215 Ser Asp Ser Ala Ser
Leu Ser Lys Ser Thr Ser Thr Ser Thr Ser 1220 1225 1230 Asp Ser Asp
Ser Thr Ser Thr Ser Leu Ser Lys Ser Thr Ser Thr 1235 1240 1245 Ser
Thr Ser Asp Ser Asp Ser Ala Ser Lys Ser Thr Ser Val Ser 1250 1255
1260 Asp Ser Thr Ser Ala Ser Leu Ser Lys Ser Thr Ser Thr Ser Thr
1265 1270 1275 Ser Asp Ser Asp Ser Ala Ser Lys Ser Thr Ser Val Ser
Asp Ser 1280 1285 1290 Thr Ser Ala Ser Leu Arg Lys Ser Ala Ser Thr
Ser Thr Ser Asp 1295 1300 1305 Ser Asp Ser Thr Ser Thr Ser Leu Ser
Lys Ser Thr Ser Thr Ser 1310 1315 1320 Thr Ser Asp Ser Asp Ser Ala
Ser Lys Ser Thr Ser Val Ser Asp 1325 1330 1335 Ser Asp Ser Ala Ser
Leu Ser Lys Ser Thr Ser Thr Ser Thr Ser 1340 1345 1350 Asp Ser Asp
Ser Ala Ser Lys Ser Thr Ser Val Ser Asp Ser Asp 1355 1360 1365 Ser
Ala Ser Leu Ser Lys Ser Thr Ser Thr Ser Thr Ser Asp Ser 1370 1375
1380 Asp Ser Ala Ser Lys Ser Thr Ser Val Ser Asp Ser Thr Ser Thr
1385 1390 1395 Ser Leu Ser Lys Ser Thr Ser Thr Ser Thr Ser Asp Ser
Asp Ser 1400 1405 1410 Ala Ser Thr Ser Thr Ser Val Ser Asp Ser Thr
Ser Ala Ser Leu 1415 1420 1425 Ser Lys Ser Thr Ser Thr Ser Thr Ser
Asp Ser Asp Ser Ala Ser 1430 1435 1440 Thr Ser Val Ser Asp Ser Asp
Ser Ala Ser Leu Ser Lys Ser Thr 1445 1450 1455 Ser Thr Ser Thr Ser
Asp Ser Asp Ser Val Ser Thr Ser Thr Ser 1460 1465 1470 Val Ser Asp
Ser Asp Ser Ala Ser Leu Ser Lys Ser Thr Ser Thr 1475 1480 1485 Ser
Thr Ser Asp Ser Asp Ser Ala Ser Lys Ser Thr Ser Val Ser 1490 1495
1500 Asp Ser Thr Ser Thr Ser Leu Ser Lys Ser Thr Ser Thr Ser Thr
1505 1510 1515 Ser Asp Ser Asp Ser Ala Ser Thr Ser Val Ser Asp Ser
Thr Ser 1520 1525 1530 Ala Ser Leu Ser Lys Ser Thr Ser Thr Ser Thr
Ser Asp Ser Asp 1535 1540 1545 Ser Ala Ser Lys Ser Thr Ser Val Ser
Asp Ser Asp Ser Ala Ser 1550 1555 1560 Leu Ser Lys Ser Thr Ser Thr
Ser Thr Ser Asp Ser Asp Ser Ala 1565 1570 1575 Ser Thr Ser Thr Ser
Val Ser Asp Ser Asp Ser Ala Ser Leu Ser 1580 1585 1590 Lys Ser Thr
Ser Thr Ser Thr Ser Asp Ser Asp Ser Ala Ser Thr 1595 1600 1605 Ser
Leu Ser Lys Ser Thr Ser Thr Ser Thr Ser Asp Ser Asp Ser 1610 1615
1620 Thr Ser Thr Ser Leu Ser Lys Ser Thr Ser Thr Ser Thr Ser Asp
1625 1630 1635 Ser Asp Ser Ala Ser Lys Ser Thr Ser Val Ser Asp Ser
Asp Ser 1640 1645 1650 Ala Ser Leu Ser Lys Ser Thr Ser Thr Ser Thr
Ser Asp Ser Asp 1655 1660 1665 Ser Ala Ser Lys Ser Thr Ser Val Ser
Asp Ser Asp Ser Ala Ser 1670 1675 1680 Leu Ser Lys Ser Thr Ser Thr
Ser Thr Ser Asp Ser Asp Ser Ala 1685 1690 1695 Ser Lys Ser Thr Ser
Val Ser Asp Ser Glu Ser Ala Ser Leu Ser 1700 1705 1710 Lys Ser Thr
Ser Thr Ser Thr Ser Asp Ser Asp Ser Ala Ser Thr 1715 1720 1725 Ser
Thr Ser Val Ser Asp Ser Thr Ser Val Ser Leu Ser Lys Ser 1730 1735
1740 Thr Ser Thr Ser Thr Ser Asp Ser Asp Ser Thr Ser Thr Ser Leu
1745 1750 1755 Ser Lys Ser Thr Ser Thr Ser Thr Ser Asp Ser Asp Ser
Asp Ser 1760 1765 1770 Ala Ser Leu Ser Lys Ser Thr Ser Thr Ser Thr
Ser Asp Ser Asp 1775 1780 1785 Ser Val Ser Thr Ser Thr Ser Val Ser
Asp Ser Asp Ser Ala Ser 1790 1795 1800 Leu Ser Lys Ser Thr Ser Thr
Ser Thr Ser Asp Ser Asp Ser Ala 1805 1810 1815 Ser Thr Ser Thr Ser
Val Ser Asp Ser Gly Ser Ala Ser Leu Ser 1820 1825 1830 Lys Ser Thr
Ser Thr Ser Thr Ser Asp Ser Asp Ser Ala Ser Lys 1835 1840 1845 Ser
Thr Ser Val Ser Asp Ser Asp Ser Ala Ser Leu Ser Lys Ser 1850 1855
1860 Thr Ser Thr Ser Thr Ser Asp Ser Asp Ser Ala Ser Thr Ser Thr
1865 1870 1875 Ser Val Ser Asp Ser Thr Ser Ala Ser Leu Ser Lys Ser
Thr Ser 1880 1885 1890 Thr Ser Thr Ser Asp Ser Asp Ser Ala Ser Thr
Ser Thr Ser Val 1895 1900 1905 Ser Asp Ser Asp Ser Ala Ser Leu Ser
Lys Ser Thr Ser Thr Ser 1910 1915 1920 Thr Ser Asp Ser Asp Ser Ala
Ser Lys Ser Thr Ser Val Ser Asp 1925 1930 1935 Ser Asp Ser Ala Ser
Leu Ser Lys Ser Thr Ser Thr Ser Thr Ser 1940 1945 1950 Glu Ser Asp
Ser Ala Ser Lys Ser Thr Ser Val Ser Asp Ser Asp 1955 1960 1965 Ser
Ala Ser Leu Ser Lys Ser Thr Ser Thr Ser Thr Ser Asp Ser 1970 1975
1980 Asp Ser Ala Ser Thr Ser Thr Ser Val Ser Asp Ser Asp Ser Ala
1985 1990 1995 Ser Leu Ser Lys Ser Thr Ser Thr Ser Thr Ser Asp Ser
Asp Ser 2000 2005 2010 Ala Ser Lys Ser Thr Ser Val Ser Asp Ser Asp
Ser Ala Ser Leu 2015 2020 2025 Ser Lys Ser Thr Ser Thr Ser Thr Ser
Glu Ser Asp Ser Ala Ser 2030 2035 2040 Thr Ser Thr Leu Val Ser Asp
Ser Thr Ser Val Ser Leu Ser Gln 2045 2050 2055 Ser Thr Ser Val Asp
Lys Asp Ser Thr Ala Lys Gly Ser Thr Glu 2060 2065 2070 Leu Val Asn
Val Ala Ser Leu Ser Ile Ser Ala Ser Gln Ser Ser 2075 2080 2085 Ser
Leu Ser Ala Ser Thr Ser Thr Ser Ile Glu Lys Ser Glu Ser 2090 2095
2100 Thr Ser Thr Ser Gly Ser Asn Ser Thr Asn Ala Ser Leu Ser Ser
2105 2110 2115 Ser Ser Ser Leu Ser Thr Ser Ala Ser Thr Ser Val Ser
Glu Val 2120 2125 2130 Thr Ser Val Thr His Ser Glu Asn Asp Leu Ser
Ala Ser Asn Asp 2135 2140 2145 Arg Asp Thr Ser Gly Ser Val Ser Gln
Phe Ala Ser Glu Asn Thr 2150 2155 2160 Ser Leu Ser Asp Ser Ala Ser
Ile Ser Gly Glu Val Ser Ser Ser 2165 2170 2175 Thr Ser Ala Ser Thr
Ser Lys Ser Ser Ser Leu Ser Ala Ser Ala 2180 2185 2190 Leu His Asp
Lys His Val Ser Glu Ser Thr Ser Ala Ser Leu Ser 2195 2200 2205 Ser
Gly Asp Ser Ser Arg Ala Ser Ala Ser Val Ser Thr Ser Leu 2210 2215
2220 Ser Glu Ser Asp Ser Ala Leu Ile Asp Ser Glu Ser Ile Ser Val
2225 2230 2235 Ser Glu His Thr Ser Thr Leu Gln Ser Gly Ser His Ser
Leu Ser 2240 2245 2250 Gln Gln Gln Ser Ala Glu Leu Ser Gln Ser Glu
Gln Thr Ser Gln 2255 2260 2265 Ser Gln Arg Ile Ser Thr Ser Ala Ser
Val Ser Ala Met Lys Ser 2270 2275 2280 Glu Ser Ala Ala Lys Val Ser
Glu Ser Leu Ser Thr Ser Gln Ser 2285 2290 2295 Lys Val Asp Ser Gln
Ser Gln Ser Val Ser Glu Ser Ala Ser Asn 2300 2305 2310 Ser Arg Val
Ser Arg Asp Ser Lys Ser Thr Ser Ala Ser Met His 2315 2320 2325 Arg
Ser Leu Ser Glu Ser Val Ser Gln Ser Met Ser Leu Ile Asp 2330 2335
2340 Gln Ser Glu Ser Asp Ser Thr Ser Ile Ser Ile Ser Thr Ser Ile
2345 2350 2355 Ser Asp Glu Asp Ser Met Leu Tyr Ser Met Ser Asp Ser
Ala Ser 2360 2365 2370 Ile Ser Thr Lys Ala Ser Ser Ser Met Ser Thr
Ser Thr Ser Glu 2375 2380 2385 Glu His Ala Asn Ser His Ser Gln Ser
Glu Ser Thr Ala Ser Val 2390 2395 2400 Glu Val Ser Gln Glu Met Ser
Ala Ser Ala Ser Thr Ser Lys Ser 2405 2410 2415 Glu Ser Gln Ser Glu
Ser Val Ser Val Ser Asn Glu Glu Ser Asn 2420 2425 2430 Ile Ser Ser
Met Gln Glu Ser Phe Val Glu Ser Ala Lys Ala Ser 2435 2440 2445 Arg
Ser Ala Ser Met Ser Val Ala Lys Ser Glu Ala Ser Glu Ser 2450 2455
2460 Gln Leu Leu Ser Glu Ser Asn Ala Ser Val Ser Gln Ser Ala Ser
2465 2470 2475 Thr Ser Ser Lys Ala Ser Ala Ser Thr Ser Glu Ser Ile
Ser Thr 2480 2485 2490 Ser Leu Ser Val Ser Glu Ala Thr His Gly Lys
Pro Arg Asn His 2495 2500 2505 Ser Glu Ser Ala Ser Ala Ser Gln Leu
Leu Glu Glu Asn Glu Ser 2510 2515 2520 Leu Ser Asp Ser Ala Ser Thr
Ser Val Glu Asp Ser Glu Ser Ala 2525 2530 2535 Ser Ala Ser Leu Ser
Val Tyr Gln Ser Gln Ser Ala Ser Ala Leu 2540 2545 2550 Lys Ser Thr
His Ala Ser Glu Lys Ala Ser Val Asn Thr Ser Ala 2555 2560 2565 Asn
Ala Ser Lys Arg Ala Ser Ala Ser Thr Ser Ile Ser Asn Ser 2570 2575
2580 Lys Ser Lys Val Ile Ala Ser Glu Ser Lys Ser Thr Ser Ile Ser
2585 2590 2595 Thr Tyr Glu Ser Leu Ser Ile Ser Thr Ser Lys Glu Gln
Ser Thr 2600 2605 2610 Arg Val Ser Val Ser Glu Ser Thr Ser Thr Ser
Lys Val Lys Ser 2615 2620 2625 Glu Ser Asp Ser Ala Ser Thr Ser Thr
Ser Glu Ser Ile Ser Ile 2630 2635 2640 Ser Ala Asn Arg Ser Gly Tyr
Thr Ser Ser Lys Arg Ser Val Gln 2645 2650 2655 Met Ser Glu Ala Gln
Ser Thr Ser Asp Ser Leu Ser Val Met Gln 2660 2665 2670 Ser Glu Gly
Ser Val Ser Val Ser Gln Ser Leu Ser Ile Ser Asp 2675 2680 2685 Lys
Thr Ser Gln Ser Leu Ser Glu Ser Ile Ser His Ser Glu Ser 2690 2695
2700 Asp Ser Asp Ser Asn Ser Val Ser Ile Ser Gln Glu Thr Ser Glu
2705 2710 2715 Gln His Ser Val Ser Asp Ser Asp Ser Met Ser Ile Ser
Glu Ser 2720 2725 2730 Glu Ser Ile Ala Tyr Ser Gln Ser Ala Ser Glu
Ser Glu Ser Thr 2735 2740
2745 Ser Ile Ala Lys Ser Asp Ser Ile Ser Asn Ser Leu Ser Val Ser
2750 2755 2760 Leu Ser Glu Ser Glu Ser Glu Ala Ser Thr Ser Ala Ser
Val Ser 2765 2770 2775 Thr Ser Glu Ser Thr Ser Val Lys Gly Ser Leu
Ser Thr Ser Ile 2780 2785 2790 Leu Asn Ser Gln Ser Ala Ser Thr His
Gln Ser Thr Glu Ala Ser 2795 2800 2805 Gln Ser Thr Ser Thr Ser Lys
Val Glu Glu Ala Ser Leu Ser Asp 2810 2815 2820 Ser Ala Ser Val Ser
Asp Ser Gln Ser Leu Ser Met Ser His Glu 2825 2830 2835 Lys Ser Gln
Ser Ala Ser Thr Ser Lys Ser Thr Ser Leu Ser Lys 2840 2845 2850 Thr
Ile Ser Glu Ser Glu Ser Val Ser Ala Ser Thr Ser Thr Ser 2855 2860
2865 Glu Ala Val Ser Thr Glu Ala Ser Glu Phe Val Ser Ala Val Asp
2870 2875 2880 Ser Leu Ser Gln Val Thr Ser Asn Gly Ser Thr Thr Lys
Glu Asp 2885 2890 2895 Ala Ser Thr Phe Val Ser Thr Val Asp Ser Leu
Lys Asp Lys Ala 2900 2905 2910 Ser Asn Asn Gly Thr Pro Ser Glu Phe
Ala Ser Ala Val Lys Ser 2915 2920 2925 Thr His Ala Ser Val Ser Val
Ser Ala Ser Glu Ser Thr Ser Ala 2930 2935 2940 Ser Thr Ser Thr Ser
Glu Ala Val Ser Thr Glu Ala Ser Glu Phe 2945 2950 2955 Val Ser Ala
Val Asn Ser Leu Ser Glu Ala Thr Ser Asn Gly Ser 2960 2965 2970 Thr
Thr Lys Glu Asp Ala Ser Thr Phe Val Ser Thr Val Asp Ser 2975 2980
2985 Leu Lys Asp Lys Ala Ser Asn Asn Gly Thr Pro Ser Glu Phe Ala
2990 2995 3000 Ser Ala Val Lys Ser Thr His Ala Ser Val Ser Val Ser
Ala Ser 3005 3010 3015 Glu Ser Thr Ser Ala Ser Thr Ser Thr Ser Glu
Ala Val Ser Thr 3020 3025 3030 Glu Ala Ser Glu Phe Val Ser Ala Val
Asp Ser Leu Ser Gln Val 3035 3040 3045 Thr Ser Asn Gly Ser Thr Thr
Lys Glu Asp Ala Ser Thr Phe Val 3050 3055 3060 Ser Thr Val Asp Ser
Leu Lys Asp Lys Ala Ser Asn Asn Gly Thr 3065 3070 3075 Pro Ser Glu
Phe Glu Ser Val Val Lys Ser Val His Gly Ser Met 3080 3085 3090 Ser
Ala Ser Ala Ser Ala Ser Thr Ser Ala Ser Thr Ser Ala Ser 3095 3100
3105 Thr Ser Thr Ser Glu Ala Ala Ser Ala Glu Ala Ser Glu Leu Glu
3110 3115 3120 Ser Val Arg Lys Ser Leu Ser Asn Gly Ala Ser Asn Gly
Ser Thr 3125 3130 3135 Ala Arg Glu Gly Ala Ser Thr Phe Val Ser Thr
Val Asp Ser Leu 3140 3145 3150 Lys Asp Lys Ala Ser Asn Asn Gly Thr
Ala Ser Glu Phe Glu Ser 3155 3160 3165 Val Val Lys Ser Val His Gly
Ser Thr Ser Ala Ser Ala Ser Ala 3170 3175 3180 Ser Thr Ser Ala Ser
Thr Ser Ala Ser Glu Ser Ala Ser Thr Glu 3185 3190 3195 Ala Ser Glu
Phe Val Ser Ala Val Ala Ser Leu Ser Ser Ser Ala 3200 3205 3210 Trp
Asn Gly Ser Thr Thr Gly Glu Gly Ala Ser Thr Phe Val Ser 3215 3220
3225 Thr Val Asp Ser Ser Lys Asp Ser Ala Ser Asp Lys Ala Ser Pro
3230 3235 3240 Ser Glu Ser Glu Ser Val Val Lys Ser Val His Gly Ser
Thr Ser 3245 3250 3255 Thr Ser Ala Ser Val Ser Ala Ser Ala Ser Thr
Ser Ala Ser Thr 3260 3265 3270 Ser Thr Ser Glu Ala Val Ser Thr Glu
Ala Ser Glu Phe Val Ser 3275 3280 3285 Ala Val Asn Ser Leu Ser Ser
Glu Ala Ser Asn Gly Ser Thr Thr 3290 3295 3300 Arg Glu Gly Ala Ser
Thr Phe Val Ser Thr Val Asp Ser Leu Lys 3305 3310 3315 Asp Lys Ala
Ser Asn Asn Gly Thr Ala Ser Glu Phe Glu Ser Val 3320 3325 3330 Val
Lys Ser Val His Gly Ser Met Ser Thr Ser Ala Ser Val Ser 3335 3340
3345 Ala Ser Glu Ser Thr Ser Ala Ser Thr Ser Thr Ser Glu Ala Val
3350 3355 3360 Ser Thr Glu Ala Ser Glu Ser Ala Ser Ile Ser Val Ser
Met Ser 3365 3370 3375 Val Ser Ala Ser Thr Ser Ala Ser Met Ser Val
Ser Val Ser Asn 3380 3385 3390 Ser Val Ser Val Ser Asp Ser Ile Ser
Val Ser Ala Ser Thr Ser 3395 3400 3405 Glu Pro Asn Ser Val Ser Thr
Ser Met Ser Ser Ser Leu Ser Thr 3410 3415 3420 Ser Ala Ser Thr Pro
Ser Glu Ile Thr Ser Ser Ser Ser Ser Ser 3425 3430 3435 Asp Ser Ala
Thr Val Gln Lys Val Val Ser Lys Asp Glu Gln His 3440 3445 3450 Ala
Thr Asn Lys Val Glu Lys Leu Pro Asp Thr Gly Gln Ser Thr 3455 3460
3465 Thr Gln Thr Gly Leu Leu Gly Gly Val Gly Ala Leu Leu Thr Gly
3470 3475 3480 Leu Gly Leu Leu Lys Lys Ser Arg Lys Gln Lys Asp Glu
Glu Thr 3485 3490 3495 Ser Ser His Glu 3500 135688DNAStaphylococcus
pseudintermedius 13atgaaaaagt ctagaaaaaa gcgtatcgat tttttaccta
accgtcaaaa tcgatatgcg 60atacgtcgtt tttcagtagg cactgcgtca attctcgttg
gagcaacatt aatttttgga 120attcattcaa atgatgcatc ggcagcagta
gaagacgcaa catctcaaga agcaggaaca 180actaacgaaa attcaaatag
tacagaagaa gcaacaacaa acgaaagtac aactgttgaa 240gcaccaacaa
gtgaagaagc aacaacggaa gagcaatcag tagaggcgcc aacaagtgaa
300gaagtaacaa cggaagagca atcagtagag gcaccaacaa gtgaagaagt
aacaacggaa 360gagcaatcag tagaagcgcc aacaagtgaa gaagtaacaa
cggaagagca atcagtagaa 420gcgccaacaa gtgaagaagt aacaacggaa
gagcaatcag tagaggcacc aacaagtgaa 480gaagtaacaa cggaagagca
atcagtagag gcaccaacta gtgaagaagt aactacggaa 540gagcaatcag
tagaagcacc aacaagtgaa gaagcaacaa cggaagagca atcagtagaa
600gcaccaacaa gtgaagaagc aactacaaaa actcctgtaa aagaagaaac
atcctcaaca 660caagaaaatt cacccacgac tacactagaa gaacaatttt
caaatgaatt caatcagtta 720acatctacag aagataaaac aaactacaca
cgtgaatatt taactcaaaa cacaaatctt 780tcggcagaac aagtggaagc
aacagttgaa cgcttgaatt taagtcaaga aaatgtaaca 840gcccaagata
tctatttcgc attacttaaa gatttagctg atcaacaaga tgccttatta
900ccacgtgtaa cacttttggc cgctagagat tctgagctca caaacgaagc
gtctatcgct 960ttaactgaaa atagtccaat gttccgcgca gcattagcga
atagtccttc tggcaatgat 1020gtggtgtcag aagaagataa tattattgtg
gctgatgcac tcgcaaatgg atacatcaat 1080tcacaaacag atgcaacaaa
tgcggcaaat acattgtctg gtcgtgcatg ggttgtggat 1140acagggacac
cagcgacaat gtcaaacggc ttaacagctg ttccagaagg cacaaaagtc
1200tacatgcaat ggattgatac agatggcgcg gtttcaccag tgtatcaagc
aagcacaaca 1260aataaattga gttcaagtgg tggtagccaa gtaggtccag
gtgcatatgc atttgattta 1320cgtgaagcat ggatagactc aaatggcaaa
gcgcacagat atgaagcgtc aagtggccaa 1380tattatcgtt tatggattga
tgactacaaa acagtagatg ggaatacggc aaccatgtta 1440cgccaagcag
gtggtttctt ccctggttca tatgttaatt cggtgacagg taacaatatt
1500ggtcaattcc cacttatcgg aacgaacatg caacgtacag gtatctttat
gggtgtgata 1560ccaacgaacg attacatgac tacagataca agcaattgga
ttcaagataa tgaaggacct 1620atttcaaacc cagcagtaac gagcacaagt
gaatttgtca gtggtaaagt atggtctgag 1680acaggttcag gtgactatgc
gaactctgcg acaggtccaa actttaactc aggtgatatt 1740gcacgtgaag
gttatcaagt tgtcatgtct tcattaacaa gtgctggtgc ccaagcgtat
1800aaagcacaag tcgaatcgtt gccaacagac caacaagcgg cagcagcaca
ccaattattc 1860aaagaccacc cagaatttat ttctgcgaca gtgacgggta
aaactgatgc aaacggtgcg 1920tatacattac gtttcccttc aggctcattg
agtaaagatt atctttatgg ttatgtgatg 1980gataataagg gcaacttggt
taagggctat tcatcattca cgtcaccttt attccgttcg 2040cctaacagta
acttatcttt cgcgccacaa acagcgccat atcatagacc agccaaaaat
2100gcttgggtga atgtgaactt tgcgcttgta gaaacaattg aaacaagtat
agacatcacg 2160aactttgatg tgacagccaa cccagcgcaa cgtggtgata
cggctatcat tgatgtgact 2220tctacagcat tgtcaccatt acctacgcat
gttgagtgga gagattcaaa agggaatgtc 2280gttcaaaaaa gtggagatgt
cactacggta gaagaagctg aaacggcagg cacatttact 2340attcctgatg
atgcgaaaac aggtgaaatc tatacagttt atattgtttc aggaggcaat
2400gaagttgcag cagactcact gattgtccaa gtgcaagaaa atgcggcaac
ctatgaacct 2460gtatatccaa caacaacagt tgaacaagac caaactgtaa
caattcctac acctacaaat 2520gaagatggtt tagcattacc agacggaaca
aagttcgaag gtggcaacaa tgtacctgaa 2580tgggcaactg tgaatgaaga
tggttctatt tcaatttcac caaatcaaga tgtggaaaaa 2640ggtaactata
atgtgcctgt tgtcgtcaca tatccagatg gttcaaaaga aacagtattt
2700gcaccagttt tagttcaaga agctgttcca actgcagaac aatacgatcc
aacaattgaa 2760acaattaata aggaatatgg tactactgca acagaagatg
aaattaaagg cgcaatcaca 2820attccggatt acccaacaga tggagatcaa
ccaacaatca cgattgacga cccaactcaa 2880attccaaatg gaacagaaga
aggcacagtg aatgtaggtg tcactgtcac ttatccagat 2940ggttcaacag
acaaattaac agtaccagtc gttacaggta agcaagcgga taacgataag
3000tacacaccag aaacaacacc aattacgaaa gacttcggta caggtgtaac
agaagacgaa 3060gtgaaaggtg cagtcactgt tccggattac ccaacagatg
gagaccaacc aacaattacg 3120attgacgacc caagtcagtt gcctgatggt
tcaaaagaag gaacaacgga tgtcgacgta 3180acagtggaat atccagacgg
cacaacagat cacatcacag ttccagtgac tgttggaaag 3240caagcggata
atgataagta cacaccagaa acaacaccaa ttacgaaaga cttcggtaca
3300ggtgtaacag aagacgaagt gaaaggtgca gtcactgttc cggattaccc
aacagacggt 3360gaccaaccaa caattacaat tgatgatcca aatcaattac
cggacggttc acaagaaggt 3420acgactgatg taaatgtaac agtggaatat
ccagatggca caacagatca catcacagtt 3480ccagtgactg ttggaaagca
agcggataat gataagtaca caccagaaac aacaccaatt 3540acgaaagact
tcggtacagg tgtaacagaa gacgaagtga aaggtgcagt cactgttccg
3600gattacccaa cagatggaga tcaaccaacg gttacaattg atgatccaaa
tcaattaccg 3660gacggttcac aagaaggtac gactgatgta aatgtaacag
tggaatatcc agacggcaca 3720acagatcaca tcacagttcc agtgactgtt
ggaaagcaag cggataatga taagtacaca 3780ccagaaacaa caccaattac
gaaagacttc ggtacaggtg taacagaaga cgaagtgaaa 3840ggtgcagtca
ctgttccgga ttacccaaca gacggtgacc aaccaacggt tacaattgat
3900gatccaaatc aattaccgga cggttcacaa gaaggtacga ctgatgtaaa
tgtaacagtg 3960gaatatccag atggcacaac agatcacatc acagttccag
tgactgttgg aaagcaagcg 4020gataacgata agtacacacc agaaacaaca
ccaattacga aagacttcgg tacaggtgta 4080acagaagacg aagtgaaagg
tgcagtcact gttccggatt acccaacaga tggagatcaa 4140ccaacggtta
caattgacga tccgagtcag ttaccagatg gctcacaaga aggcacaaca
4200gatgtgaatg taacagtgga atatccagat ggcacaacag accacatcac
agttccagtg 4260actgttggta agcaagcaga taacgataag tacacgccag
aaacaacacc aattacgaaa 4320gacttcggta caggtgtaac agaagacgaa
gtgaaaggtg cagtcactgt tccggattac 4380ccaacagatg gagaccaacc
aacaattaca attgacgatc cgagtcagtt accagacggt 4440tcacaagaag
gtacgactga tgtaaatgta acagtggaat atccagatgg cacaacagat
4500cacatcacag ttccagtgac tgttggtaag caagcagata acgataagta
cacaccagaa 4560acaacaccaa ttacgaaaga cttcggtaca ggtgtaacag
aagacgaagt gaaaggtgca 4620gtcactgttc cggattaccc aacagatgga
gaccaaccaa caattacaat tgacgatccg 4680agtcagttac cagacggttc
acaagaaggt acgactgatg taaatgtaac agtggaatat 4740ccagatggca
caacagatca catcacagtt ccagtgactg ttggaaagca agcagataac
4800gataagtaca caccagaaac aacaccaatt acgaaagact tcggtacagg
tgtaacagaa 4860ggcgaagtga aagattcaat cacaattccc ggttacccaa
cagatggaga ccaaccaaca 4920attacaattg acgacccaag tcagttacca
gatggttcac aagaaggtac gactgatgtc 4980gatgtaacag tggaatatcc
agacggcaca acagatcaca ttacagttcc agtgactgtt 5040ggaaagcaag
cagataacga taagtacaca ccagaaacag aaggtgtcaa caaagatcat
5100ggtacgtcag taacagaaga tgaagtgaaa ggtgcagtca ctgttccggg
atacccaaca 5160gatggagatc aaccaacggt tacaattgat gatccaagtc
aattgccgga cggttcacaa 5220gaaggtacga ctgatgtaaa tgtaacagtg
gaatatccag acggcacaac agaccacatt 5280acagtcccag taactgttgg
taaacaacct actaaagata acggggctac agataatgat 5340ggcgacatga
atcaaggcac agatgaagga aatagtgcta ctgatcatgg cgacaatgta
5400aaacaagatt caaacggaaa ctatacgccg gttgaacaac gtgacaatca
tgcgacttca 5460cctgcaacag atatggatcc aatgccaagc aatagccaaa
caacttttga tggcataaat 5520gcaaaaggtt caacttcaga gaaagcaaac
cataaacaac agtctgagca attaccagac 5580acaggtgaaa gcaatacaca
aaatggtgca cttttaggcg gattatttgc agcacttgga 5640ggcttattct
taatcggcag acgtcgtaaa gaaaaagaag gcaaataa
5688141895PRTStaphylococcus pseudintermedius 14Met Lys Lys Ser Arg
Lys Lys Arg Ile Asp Phe Leu Pro Asn Arg Gln 1 5 10 15 Asn Arg Tyr
Ala Ile Arg Arg Phe Ser Val Gly Thr Ala Ser Ile Leu 20 25 30 Val
Gly Ala Thr Leu Ile Phe Gly Ile His Ser Asn Asp Ala Ser Ala 35 40
45 Ala Val Glu Asp Ala Thr Ser Gln Glu Ala Gly Thr Thr Asn Glu Asn
50 55 60 Ser Asn Ser Thr Glu Glu Ala Thr Thr Asn Glu Ser Thr Thr
Val Glu 65 70 75 80 Ala Pro Thr Ser Glu Glu Ala Thr Thr Glu Glu Gln
Ser Val Glu Ala 85 90 95 Pro Thr Ser Glu Glu Val Thr Thr Glu Glu
Gln Ser Val Glu Ala Pro 100 105 110 Thr Ser Glu Glu Val Thr Thr Glu
Glu Gln Ser Val Glu Ala Pro Thr 115 120 125 Ser Glu Glu Val Thr Thr
Glu Glu Gln Ser Val Glu Ala Pro Thr Ser 130 135 140 Glu Glu Val Thr
Thr Glu Glu Gln Ser Val Glu Ala Pro Thr Ser Glu 145 150 155 160 Glu
Val Thr Thr Glu Glu Gln Ser Val Glu Ala Pro Thr Ser Glu Glu 165 170
175 Val Thr Thr Glu Glu Gln Ser Val Glu Ala Pro Thr Ser Glu Glu Ala
180 185 190 Thr Thr Glu Glu Gln Ser Val Glu Ala Pro Thr Ser Glu Glu
Ala Thr 195 200 205 Thr Lys Thr Pro Val Lys Glu Glu Thr Ser Ser Thr
Gln Glu Asn Ser 210 215 220 Pro Thr Thr Thr Leu Glu Glu Gln Phe Ser
Asn Glu Phe Asn Gln Leu 225 230 235 240 Thr Ser Thr Glu Asp Lys Thr
Asn Tyr Thr Arg Glu Tyr Leu Thr Gln 245 250 255 Asn Thr Asn Leu Ser
Ala Glu Gln Val Glu Ala Thr Val Glu Arg Leu 260 265 270 Asn Leu Ser
Gln Glu Asn Val Thr Ala Gln Asp Ile Tyr Phe Ala Leu 275 280 285 Leu
Lys Asp Leu Ala Asp Gln Gln Asp Ala Leu Leu Pro Arg Val Thr 290 295
300 Leu Leu Ala Ala Arg Asp Ser Glu Leu Thr Asn Glu Ala Ser Ile Ala
305 310 315 320 Leu Thr Glu Asn Ser Pro Met Phe Arg Ala Ala Leu Ala
Asn Ser Pro 325 330 335 Ser Gly Asn Asp Val Val Ser Glu Glu Asp Asn
Ile Ile Val Ala Asp 340 345 350 Ala Leu Ala Asn Gly Tyr Ile Asn Ser
Gln Thr Asp Ala Thr Asn Ala 355 360 365 Ala Asn Thr Leu Ser Gly Arg
Ala Trp Val Val Asp Thr Gly Thr Pro 370 375 380 Ala Thr Met Ser Asn
Gly Leu Thr Ala Val Pro Glu Gly Thr Lys Val 385 390 395 400 Tyr Met
Gln Trp Ile Asp Thr Asp Gly Ala Val Ser Pro Val Tyr Gln 405 410 415
Ala Ser Thr Thr Asn Lys Leu Ser Ser Ser Gly Gly Ser Gln Val Gly 420
425 430 Pro Gly Ala Tyr Ala Phe Asp Leu Arg Glu Ala Trp Ile Asp Ser
Asn 435 440 445 Gly Lys Ala His Arg Tyr Glu Ala Ser Ser Gly Gln Tyr
Tyr Arg Leu 450 455 460 Trp Ile Asp Asp Tyr Lys Thr Val Asp Gly Asn
Thr Ala Thr Met Leu 465 470 475 480 Arg Gln Ala Gly Gly Phe Phe Pro
Gly Ser Tyr Val Asn Ser Val Thr 485 490 495 Gly Asn Asn Ile Gly Gln
Phe Pro Leu Ile Gly Thr Asn Met Gln Arg 500 505 510 Thr Gly Ile Phe
Met Gly Val Ile Pro Thr Asn Asp Tyr Met Thr Thr 515 520 525 Asp Thr
Ser Asn Trp Ile Gln Asp Asn Glu Gly Pro Ile Ser Asn Pro 530 535 540
Ala Val Thr Ser Thr Ser Glu Phe Val Ser Gly Lys Val Trp Ser Glu 545
550 555 560 Thr Gly Ser Gly Asp Tyr Ala Asn Ser Ala Thr Gly Pro Asn
Phe Asn 565 570 575 Ser Gly Asp Ile Ala Arg Glu Gly Tyr Gln Val Val
Met Ser Ser Leu 580 585 590 Thr Ser Ala Gly Ala Gln Ala Tyr Lys Ala
Gln Val Glu Ser Leu Pro 595 600 605 Thr Asp Gln Gln Ala Ala Ala Ala
His Gln Leu Phe Lys Asp His Pro 610 615 620 Glu Phe Ile Ser Ala
Thr
Val Thr Gly Lys Thr Asp Ala Asn Gly Ala 625 630 635 640 Tyr Thr Leu
Arg Phe Pro Ser Gly Ser Leu Ser Lys Asp Tyr Leu Tyr 645 650 655 Gly
Tyr Val Met Asp Asn Lys Gly Asn Leu Val Lys Gly Tyr Ser Ser 660 665
670 Phe Thr Ser Pro Leu Phe Arg Ser Pro Asn Ser Asn Leu Ser Phe Ala
675 680 685 Pro Gln Thr Ala Pro Tyr His Arg Pro Ala Lys Asn Ala Trp
Val Asn 690 695 700 Val Asn Phe Ala Leu Val Glu Thr Ile Glu Thr Ser
Ile Asp Ile Thr 705 710 715 720 Asn Phe Asp Val Thr Ala Asn Pro Ala
Gln Arg Gly Asp Thr Ala Ile 725 730 735 Ile Asp Val Thr Ser Thr Ala
Leu Ser Pro Leu Pro Thr His Val Glu 740 745 750 Trp Arg Asp Ser Lys
Gly Asn Val Val Gln Lys Ser Gly Asp Val Thr 755 760 765 Thr Val Glu
Glu Ala Glu Thr Ala Gly Thr Phe Thr Ile Pro Asp Asp 770 775 780 Ala
Lys Thr Gly Glu Ile Tyr Thr Val Tyr Ile Val Ser Gly Gly Asn 785 790
795 800 Glu Val Ala Ala Asp Ser Leu Ile Val Gln Val Gln Glu Asn Ala
Ala 805 810 815 Thr Tyr Glu Pro Val Tyr Pro Thr Thr Thr Val Glu Gln
Asp Gln Thr 820 825 830 Val Thr Ile Pro Thr Pro Thr Asn Glu Asp Gly
Leu Ala Leu Pro Asp 835 840 845 Gly Thr Lys Phe Glu Gly Gly Asn Asn
Val Pro Glu Trp Ala Thr Val 850 855 860 Asn Glu Asp Gly Ser Ile Ser
Ile Ser Pro Asn Gln Asp Val Glu Lys 865 870 875 880 Gly Asn Tyr Asn
Val Pro Val Val Val Thr Tyr Pro Asp Gly Ser Lys 885 890 895 Glu Thr
Val Phe Ala Pro Val Leu Val Gln Glu Ala Val Pro Thr Ala 900 905 910
Glu Gln Tyr Asp Pro Thr Ile Glu Thr Ile Asn Lys Glu Tyr Gly Thr 915
920 925 Thr Ala Thr Glu Asp Glu Ile Lys Gly Ala Ile Thr Ile Pro Asp
Tyr 930 935 940 Pro Thr Asp Gly Asp Gln Pro Thr Ile Thr Ile Asp Asp
Pro Thr Gln 945 950 955 960 Ile Pro Asn Gly Thr Glu Glu Gly Thr Val
Asn Val Gly Val Thr Val 965 970 975 Thr Tyr Pro Asp Gly Ser Thr Asp
Lys Leu Thr Val Pro Val Val Thr 980 985 990 Gly Lys Gln Ala Asp Asn
Asp Lys Tyr Thr Pro Glu Thr Thr Pro Ile 995 1000 1005 Thr Lys Asp
Phe Gly Thr Gly Val Thr Glu Asp Glu Val Lys Gly 1010 1015 1020 Ala
Val Thr Val Pro Asp Tyr Pro Thr Asp Gly Asp Gln Pro Thr 1025 1030
1035 Ile Thr Ile Asp Asp Pro Ser Gln Leu Pro Asp Gly Ser Lys Glu
1040 1045 1050 Gly Thr Thr Asp Val Asp Val Thr Val Glu Tyr Pro Asp
Gly Thr 1055 1060 1065 Thr Asp His Ile Thr Val Pro Val Thr Val Gly
Lys Gln Ala Asp 1070 1075 1080 Asn Asp Lys Tyr Thr Pro Glu Thr Thr
Pro Ile Thr Lys Asp Phe 1085 1090 1095 Gly Thr Gly Val Thr Glu Asp
Glu Val Lys Gly Ala Val Thr Val 1100 1105 1110 Pro Asp Tyr Pro Thr
Asp Gly Asp Gln Pro Thr Ile Thr Ile Asp 1115 1120 1125 Asp Pro Asn
Gln Leu Pro Asp Gly Ser Gln Glu Gly Thr Thr Asp 1130 1135 1140 Val
Asn Val Thr Val Glu Tyr Pro Asp Gly Thr Thr Asp His Ile 1145 1150
1155 Thr Val Pro Val Thr Val Gly Lys Gln Ala Asp Asn Asp Lys Tyr
1160 1165 1170 Thr Pro Glu Thr Thr Pro Ile Thr Lys Asp Phe Gly Thr
Gly Val 1175 1180 1185 Thr Glu Asp Glu Val Lys Gly Ala Val Thr Val
Pro Asp Tyr Pro 1190 1195 1200 Thr Asp Gly Asp Gln Pro Thr Val Thr
Ile Asp Asp Pro Asn Gln 1205 1210 1215 Leu Pro Asp Gly Ser Gln Glu
Gly Thr Thr Asp Val Asn Val Thr 1220 1225 1230 Val Glu Tyr Pro Asp
Gly Thr Thr Asp His Ile Thr Val Pro Val 1235 1240 1245 Thr Val Gly
Lys Gln Ala Asp Asn Asp Lys Tyr Thr Pro Glu Thr 1250 1255 1260 Thr
Pro Ile Thr Lys Asp Phe Gly Thr Gly Val Thr Glu Asp Glu 1265 1270
1275 Val Lys Gly Ala Val Thr Val Pro Asp Tyr Pro Thr Asp Gly Asp
1280 1285 1290 Gln Pro Thr Val Thr Ile Asp Asp Pro Asn Gln Leu Pro
Asp Gly 1295 1300 1305 Ser Gln Glu Gly Thr Thr Asp Val Asn Val Thr
Val Glu Tyr Pro 1310 1315 1320 Asp Gly Thr Thr Asp His Ile Thr Val
Pro Val Thr Val Gly Lys 1325 1330 1335 Gln Ala Asp Asn Asp Lys Tyr
Thr Pro Glu Thr Thr Pro Ile Thr 1340 1345 1350 Lys Asp Phe Gly Thr
Gly Val Thr Glu Asp Glu Val Lys Gly Ala 1355 1360 1365 Val Thr Val
Pro Asp Tyr Pro Thr Asp Gly Asp Gln Pro Thr Val 1370 1375 1380 Thr
Ile Asp Asp Pro Ser Gln Leu Pro Asp Gly Ser Gln Glu Gly 1385 1390
1395 Thr Thr Asp Val Asn Val Thr Val Glu Tyr Pro Asp Gly Thr Thr
1400 1405 1410 Asp His Ile Thr Val Pro Val Thr Val Gly Lys Gln Ala
Asp Asn 1415 1420 1425 Asp Lys Tyr Thr Pro Glu Thr Thr Pro Ile Thr
Lys Asp Phe Gly 1430 1435 1440 Thr Gly Val Thr Glu Asp Glu Val Lys
Gly Ala Val Thr Val Pro 1445 1450 1455 Asp Tyr Pro Thr Asp Gly Asp
Gln Pro Thr Ile Thr Ile Asp Asp 1460 1465 1470 Pro Ser Gln Leu Pro
Asp Gly Ser Gln Glu Gly Thr Thr Asp Val 1475 1480 1485 Asn Val Thr
Val Glu Tyr Pro Asp Gly Thr Thr Asp His Ile Thr 1490 1495 1500 Val
Pro Val Thr Val Gly Lys Gln Ala Asp Asn Asp Lys Tyr Thr 1505 1510
1515 Pro Glu Thr Thr Pro Ile Thr Lys Asp Phe Gly Thr Gly Val Thr
1520 1525 1530 Glu Asp Glu Val Lys Gly Ala Val Thr Val Pro Asp Tyr
Pro Thr 1535 1540 1545 Asp Gly Asp Gln Pro Thr Ile Thr Ile Asp Asp
Pro Ser Gln Leu 1550 1555 1560 Pro Asp Gly Ser Gln Glu Gly Thr Thr
Asp Val Asn Val Thr Val 1565 1570 1575 Glu Tyr Pro Asp Gly Thr Thr
Asp His Ile Thr Val Pro Val Thr 1580 1585 1590 Val Gly Lys Gln Ala
Asp Asn Asp Lys Tyr Thr Pro Glu Thr Thr 1595 1600 1605 Pro Ile Thr
Lys Asp Phe Gly Thr Gly Val Thr Glu Gly Glu Val 1610 1615 1620 Lys
Asp Ser Ile Thr Ile Pro Gly Tyr Pro Thr Asp Gly Asp Gln 1625 1630
1635 Pro Thr Ile Thr Ile Asp Asp Pro Ser Gln Leu Pro Asp Gly Ser
1640 1645 1650 Gln Glu Gly Thr Thr Asp Val Asp Val Thr Val Glu Tyr
Pro Asp 1655 1660 1665 Gly Thr Thr Asp His Ile Thr Val Pro Val Thr
Val Gly Lys Gln 1670 1675 1680 Ala Asp Asn Asp Lys Tyr Thr Pro Glu
Thr Glu Gly Val Asn Lys 1685 1690 1695 Asp His Gly Thr Ser Val Thr
Glu Asp Glu Val Lys Gly Ala Val 1700 1705 1710 Thr Val Pro Gly Tyr
Pro Thr Asp Gly Asp Gln Pro Thr Val Thr 1715 1720 1725 Ile Asp Asp
Pro Ser Gln Leu Pro Asp Gly Ser Gln Glu Gly Thr 1730 1735 1740 Thr
Asp Val Asn Val Thr Val Glu Tyr Pro Asp Gly Thr Thr Asp 1745 1750
1755 His Ile Thr Val Pro Val Thr Val Gly Lys Gln Pro Thr Lys Asp
1760 1765 1770 Asn Gly Ala Thr Asp Asn Asp Gly Asp Met Asn Gln Gly
Thr Asp 1775 1780 1785 Glu Gly Asn Ser Ala Thr Asp His Gly Asp Asn
Val Lys Gln Asp 1790 1795 1800 Ser Asn Gly Asn Tyr Thr Pro Val Glu
Gln Arg Asp Asn His Ala 1805 1810 1815 Thr Ser Pro Ala Thr Asp Met
Asp Pro Met Pro Ser Asn Ser Gln 1820 1825 1830 Thr Thr Phe Asp Gly
Ile Asn Ala Lys Gly Ser Thr Ser Glu Lys 1835 1840 1845 Ala Asn His
Lys Gln Gln Ser Glu Gln Leu Pro Asp Thr Gly Glu 1850 1855 1860 Ser
Asn Thr Gln Asn Gly Ala Leu Leu Gly Gly Leu Phe Ala Ala 1865 1870
1875 Leu Gly Gly Leu Phe Leu Ile Gly Arg Arg Arg Lys Glu Lys Glu
1880 1885 1890 Gly Lys 1895 151860DNAStaphylococcus
pseudintermedius 15atgacagaac gaaaatcccc ttcatctcaa aacatgcgtc
atcgtttagt caaagctggt 60actgtccttt tattggttgg tagtggactg caaatgcctt
caacattgtc acacgaaatg 120acagcgatag ctcagacaga tgcgactgat
gatttgaaaa cattacgtga aaatgcagat 180aaaaaagtga aagcgttaca
atatttaaat acggattata aaaatgaatt tcttgcgtta 240attcgtgaat
atgatacgtc gtcaaaaaat attgaagtgg ttgttgacga agcagaagca
300gccaatcgtc tagctcatga cgctcaatcg gacgatgaaa tacaacctga
attagatgcc 360attgatgaaa aaattagcgc gttaaaggca aaggttgatg
aaggtcaacg agaatcaact 420gaagcgcgtc aagatgtaac gtcaacagag
acaaagagtg ctgaatcaga aggaagagag 480ccatccactg aaggcgagag
caaagtaaag gagtcatctt cagcacaaac gattgtagca 540cctcatcatg
gtcaacaaga tgtgagcgca ctgaaagacc atattaagaa cgatgtcgat
600acacttaaac aagactatgc aacgcaagac aagcaagtga caccactcca
gggcattgac 660agtgcaatca cacgcattga ccatttcgtt tcagaaagcg
tggatcacaa gtctgacaat 720tattttgaag aaaaacgtca acatttacaa
aactttgaac aagacattaa aaaacgtacg 780gacatttctg ggactgagaa
ggcgactttg cttgatgatg cgaaaacggt agccaaccaa 840ctgaacgcgc
aaaatgatac gattttaact gaacttcaac agcatgacga taaacgtgca
900gcagttgaat cgatattagg tgagattttt aatgcacaag aagcggctga
acgtgcgaaa 960cagatagatg ttaaaggtaa aacagatcaa caattggcaa
acgaaattca tcaacaagcg 1020gacggactta tcaaaacgtc gagtgatgat
ttattgttag gaatgttgga aaataattca 1080aatacacaag gtctagtgga
aagcatttta cgaacacgct ttgacaaaca agaagcgcac 1140aaaattgccg
gcgaaatcat gcaaggcaag ccttcaaata cagcgatact cgaccgcttg
1200aaagaccatt ttaaagcgaa tggtaaggcg agtggagatg atattttaaa
tgcgttaatt 1260aataatacgg atgcagatgc tgaagtgatt gaatcaattc
tagggggccg tcttaatgca 1320gaaaatgcaa aattgattgc cgatcgtgta
cagcaagata aaaagaagac acatcaaaac 1380ttaaaggcga ttgaagacga
acttagtgcg caagcgaatc gattgttaac gttacggaag 1440caattgcaac
aaatccgtca taatacgcaa acagatatga atgacttgtt tgcaccactg
1500cgtcgtattg caaatattct cggtggtggt ttaaatcgtg acgacattca
ctcttcaggt 1560cgtacgaatg acaaattgca gcaactgtta aatcgtgatc
attcgttgtt aggtcgtggt 1620ggtgatttat tcaaacatga ttttgcgcca
aagccgaata tcgatccata tcaagcgatt 1680aatagtcaaa cggcatcaga
aggtttttta gatggtttat ttgatcaaaa tggcgatttc 1740aatttaccga
atacaggtga aatagtgaag cggacttggc taccgttggg tattttagtc
1800gttgcaatcg gtgtactgat cttaacggtg agatttcata aaaaaacacg
caaacaataa 186016619PRTStaphylococcus pseudintermedius 16Met Thr
Glu Arg Lys Ser Pro Ser Ser Gln Asn Met Arg His Arg Leu 1 5 10 15
Val Lys Ala Gly Thr Val Leu Leu Leu Val Gly Ser Gly Leu Gln Met 20
25 30 Pro Ser Thr Leu Ser His Glu Met Thr Ala Ile Ala Gln Thr Asp
Ala 35 40 45 Thr Asp Asp Leu Lys Thr Leu Arg Glu Asn Ala Asp Lys
Lys Val Lys 50 55 60 Ala Leu Gln Tyr Leu Asn Thr Asp Tyr Lys Asn
Glu Phe Leu Ala Leu 65 70 75 80 Ile Arg Glu Tyr Asp Thr Ser Ser Lys
Asn Ile Glu Val Val Val Asp 85 90 95 Glu Ala Glu Ala Ala Asn Arg
Leu Ala His Asp Ala Gln Ser Asp Asp 100 105 110 Glu Ile Gln Pro Glu
Leu Asp Ala Ile Asp Glu Lys Ile Ser Ala Leu 115 120 125 Lys Ala Lys
Val Asp Glu Gly Gln Arg Glu Ser Thr Glu Ala Arg Gln 130 135 140 Asp
Val Thr Ser Thr Glu Thr Lys Ser Ala Glu Ser Glu Gly Arg Glu 145 150
155 160 Pro Ser Thr Glu Gly Glu Ser Lys Val Lys Glu Ser Ser Ser Ala
Gln 165 170 175 Thr Ile Val Ala Pro His His Gly Gln Gln Asp Val Ser
Ala Leu Lys 180 185 190 Asp His Ile Lys Asn Asp Val Asp Thr Leu Lys
Gln Asp Tyr Ala Thr 195 200 205 Gln Asp Lys Gln Val Thr Pro Leu Gln
Gly Ile Asp Ser Ala Ile Thr 210 215 220 Arg Ile Asp His Phe Val Ser
Glu Ser Val Asp His Lys Ser Asp Asn 225 230 235 240 Tyr Phe Glu Glu
Lys Arg Gln His Leu Gln Asn Phe Glu Gln Asp Ile 245 250 255 Lys Lys
Arg Thr Asp Ile Ser Gly Thr Glu Lys Ala Thr Leu Leu Asp 260 265 270
Asp Ala Lys Thr Val Ala Asn Gln Leu Asn Ala Gln Asn Asp Thr Ile 275
280 285 Leu Thr Glu Leu Gln Gln His Asp Asp Lys Arg Ala Ala Val Glu
Ser 290 295 300 Ile Leu Gly Glu Ile Phe Asn Ala Gln Glu Ala Ala Glu
Arg Ala Lys 305 310 315 320 Gln Ile Asp Val Lys Gly Lys Thr Asp Gln
Gln Leu Ala Asn Glu Ile 325 330 335 His Gln Gln Ala Asp Gly Leu Ile
Lys Thr Ser Ser Asp Asp Leu Leu 340 345 350 Leu Gly Met Leu Glu Asn
Asn Ser Asn Thr Gln Gly Leu Val Glu Ser 355 360 365 Ile Leu Arg Thr
Arg Phe Asp Lys Gln Glu Ala His Lys Ile Ala Gly 370 375 380 Glu Ile
Met Gln Gly Lys Pro Ser Asn Thr Ala Ile Leu Asp Arg Leu 385 390 395
400 Lys Asp His Phe Lys Ala Asn Gly Lys Ala Ser Gly Asp Asp Ile Leu
405 410 415 Asn Ala Leu Ile Asn Asn Thr Asp Ala Asp Ala Glu Val Ile
Glu Ser 420 425 430 Ile Leu Gly Gly Arg Leu Asn Ala Glu Asn Ala Lys
Leu Ile Ala Asp 435 440 445 Arg Val Gln Gln Asp Lys Lys Lys Thr His
Gln Asn Leu Lys Ala Ile 450 455 460 Glu Asp Glu Leu Ser Ala Gln Ala
Asn Arg Leu Leu Thr Leu Arg Lys 465 470 475 480 Gln Leu Gln Gln Ile
Arg His Asn Thr Gln Thr Asp Met Asn Asp Leu 485 490 495 Phe Ala Pro
Leu Arg Arg Ile Ala Asn Ile Leu Gly Gly Gly Leu Asn 500 505 510 Arg
Asp Asp Ile His Ser Ser Gly Arg Thr Asn Asp Lys Leu Gln Gln 515 520
525 Leu Leu Asn Arg Asp His Ser Leu Leu Gly Arg Gly Gly Asp Leu Phe
530 535 540 Lys His Asp Phe Ala Pro Lys Pro Asn Ile Asp Pro Tyr Gln
Ala Ile 545 550 555 560 Asn Ser Gln Thr Ala Ser Glu Gly Phe Leu Asp
Gly Leu Phe Asp Gln 565 570 575 Asn Gly Asp Phe Asn Leu Pro Asn Thr
Gly Glu Ile Val Lys Arg Thr 580 585 590 Trp Leu Pro Leu Gly Ile Leu
Val Val Ala Ile Gly Val Leu Ile Leu 595 600 605 Thr Val Arg Phe His
Lys Lys Thr Arg Lys Gln 610 615 17729DNAStaphylococcus
pseudintermedius 17atgttaaaaa aattaattgt tacaggtttg attgctacag
cggcgacaca agtttatgcg 60catgacacgc aagcggcgga aaagggtgct acagatgctc
cgaatgtgat ggttaaggat 120gaggcgaaaa aagaagtgac accgataatc
cataaaccga cttgcattta cccgcatcta 180gaaggcgaag atgatgctgc
gtatttaaaa cgtatggcaa cgaatccacc agaaggcgca 240gtgccgtacg
gtgtattgaa taaagatgga tcgattacag aaccgaatac aaatccacat
300tttgatgttt taaaaattga agatccaaat gcgatgaaag atttggttga
tacaccggca 360gatgatcaag atacggtacc gagtgattta caaattgaac
caccagcatt aataggacca 420gctactaaac atacggatgg tacgggagac
gcaaaatcta atgatgacca
caaagtaaca 480aaatcttcgg gagcgtcagc ccaagatatg aagaaaaaag
acgtgacaac acaaactgca 540caaccaaaag cagataaaaa gatggcgact
gcaaaagtag caccagcgaa acaacaagat 600aaagcagcca aaatgttacc
agcagcaggg gaaccacaag tgaatgcaat cagtcaaaca 660gcacttgcac
tttcaatgat cgcattaggt gtcatcgcgt tctttacacg acgacgcaaa 720acaaattaa
72918242PRTStaphylococcus pseudintermedius 18Met Leu Lys Lys Leu
Ile Val Thr Gly Leu Ile Ala Thr Ala Ala Thr 1 5 10 15 Gln Val Tyr
Ala His Asp Thr Gln Ala Ala Glu Lys Gly Ala Thr Asp 20 25 30 Ala
Pro Asn Val Met Val Lys Asp Glu Ala Lys Lys Glu Val Thr Pro 35 40
45 Ile Ile His Lys Pro Thr Cys Ile Tyr Pro His Leu Glu Gly Glu Asp
50 55 60 Asp Ala Ala Tyr Leu Lys Arg Met Ala Thr Asn Pro Pro Glu
Gly Ala 65 70 75 80 Val Pro Tyr Gly Val Leu Asn Lys Asp Gly Ser Ile
Thr Glu Pro Asn 85 90 95 Thr Asn Pro His Phe Asp Val Leu Lys Ile
Glu Asp Pro Asn Ala Met 100 105 110 Lys Asp Leu Val Asp Thr Pro Ala
Asp Asp Gln Asp Thr Val Pro Ser 115 120 125 Asp Leu Gln Ile Glu Pro
Pro Ala Leu Ile Gly Pro Ala Thr Lys His 130 135 140 Thr Asp Gly Thr
Gly Asp Ala Lys Ser Asn Asp Asp His Lys Val Thr 145 150 155 160 Lys
Ser Ser Gly Ala Ser Ala Gln Asp Met Lys Lys Lys Asp Val Thr 165 170
175 Thr Gln Thr Ala Gln Pro Lys Ala Asp Lys Lys Met Ala Thr Ala Lys
180 185 190 Val Ala Pro Ala Lys Gln Gln Asp Lys Ala Ala Lys Met Leu
Pro Ala 195 200 205 Ala Gly Glu Pro Gln Val Asn Ala Ile Ser Gln Thr
Ala Leu Ala Leu 210 215 220 Ser Met Ile Ala Leu Gly Val Ile Ala Phe
Phe Thr Arg Arg Arg Lys 225 230 235 240 Thr Asn
191362DNAStaphylococcus pseudintermedius 19atggtagaat ataaaaaaga
acatagcgta aagcgactat taaaattagg aatcggttca 60acgagtattt tatgtgttgt
atcacctctt ttattaacac atgacgttgt tcaagcagca 120gatatcaata
acaggatgcc agctttgaat acattgaaga ccacttcttc atatgatcaa
180agggcacaca tggatgaatt acgaaacgcc attacttcag atagtgacac
tactcaaaca 240ccatcattca atgagataac tgtgtcttca actaatgaaa
cggatgcagc gtcaacggaa 300aatgtgaacc cgagtgatga ggtcccggca
aaggatgaaa gtgaatcaac gacaccgagt 360acagaacaag acacatctat
agaagaaacg ggtactgaag aagtgccatc tcatgaagac 420aatcatcaca
acaccccaag tcaagaagag caaccgtctc cgcctgatca accaggaaca
480aacaaagatg aagagagtgg agaaaaaccg aataaagaaa atcatcggaa
gccgaatcaa 540ccgaacaaag accaaccttc aaaagatgag aataaaaaac
ctgacaaagg aaacaaacca 600gcaccaccgt ctaaaatgcc aaatcgcccg
gatcaaaagg aagatggttc aaacaacacc 660ccaccacctg ccactgataa
cggtggaaac agtaatgacg gtacaacaac gggtcccaat 720ggtggaggtg
gcagtgaagc aagtccacca ccgaatgagc aaccgtcaaa tggcaatgca
780agcgataccc atcaaaacgg ttcagtttca agcaccaatc attcgaatca
gtatggtaca 840tcggcttatg atgaatacgc aggtttattg aataataatt
ataaatataa tccattgttt 900aaagaagagg ttgcgcgttt aagtcaattt
ggaagtcaag atcaacatga tattgcaagt 960ttgagtcgta aagaacaatt
ttctcaaaat gcatttttag atgacttgca acaaagtaca 1020gattatttta
gatatcaata ttttaacccg ctttccacag agcaatacta tcatcgttta
1080gataaacaag tattagcact cgttacgggg gaatttggtt cgatgccaga
tttcaagaaa 1140agtggtgata agtcattggt taataagcat cagcaagata
aagtgaagaa aattgaacag 1200caaggagaaa atattaatac gcatcatatg
aaaaatacga aagaagatac aggaaaatca 1260ttaagttaca agccgatgat
atatattggc attgtcatgg tcggttttgt cggcctgatc 1320agtatgattt
tatggaaacg actgcatcat ttttggaaat aa 136220453PRTStaphylococcus
pseudintermedius 20Met Val Glu Tyr Lys Lys Glu His Ser Val Lys Arg
Leu Leu Lys Leu 1 5 10 15 Gly Ile Gly Ser Thr Ser Ile Leu Cys Val
Val Ser Pro Leu Leu Leu 20 25 30 Thr His Asp Val Val Gln Ala Ala
Asp Ile Asn Asn Arg Met Pro Ala 35 40 45 Leu Asn Thr Leu Lys Thr
Thr Ser Ser Tyr Asp Gln Arg Ala His Met 50 55 60 Asp Glu Leu Arg
Asn Ala Ile Thr Ser Asp Ser Asp Thr Thr Gln Thr 65 70 75 80 Pro Ser
Phe Asn Glu Ile Thr Val Ser Ser Thr Asn Glu Thr Asp Ala 85 90 95
Ala Ser Thr Glu Asn Val Asn Pro Ser Asp Glu Val Pro Ala Lys Asp 100
105 110 Glu Ser Glu Ser Thr Thr Pro Ser Thr Glu Gln Asp Thr Ser Ile
Glu 115 120 125 Glu Thr Gly Thr Glu Glu Val Pro Ser His Glu Asp Asn
His His Asn 130 135 140 Thr Pro Ser Gln Glu Glu Gln Pro Ser Pro Pro
Asp Gln Pro Gly Thr 145 150 155 160 Asn Lys Asp Glu Glu Ser Gly Glu
Lys Pro Asn Lys Glu Asn His Arg 165 170 175 Lys Pro Asn Gln Pro Asn
Lys Asp Gln Pro Ser Lys Asp Glu Asn Lys 180 185 190 Lys Pro Asp Lys
Gly Asn Lys Pro Ala Pro Pro Ser Lys Met Pro Asn 195 200 205 Arg Pro
Asp Gln Lys Glu Asp Gly Ser Asn Asn Thr Pro Pro Pro Ala 210 215 220
Thr Asp Asn Gly Gly Asn Ser Asn Asp Gly Thr Thr Thr Gly Pro Asn 225
230 235 240 Gly Gly Gly Gly Ser Glu Ala Ser Pro Pro Pro Asn Glu Gln
Pro Ser 245 250 255 Asn Gly Asn Ala Ser Asp Thr His Gln Asn Gly Ser
Val Ser Ser Thr 260 265 270 Asn His Ser Asn Gln Tyr Gly Thr Ser Ala
Tyr Asp Glu Tyr Ala Gly 275 280 285 Leu Leu Asn Asn Asn Tyr Lys Tyr
Asn Pro Leu Phe Lys Glu Glu Val 290 295 300 Ala Arg Leu Ser Gln Phe
Gly Ser Gln Asp Gln His Asp Ile Ala Ser 305 310 315 320 Leu Ser Arg
Lys Glu Gln Phe Ser Gln Asn Ala Phe Leu Asp Asp Leu 325 330 335 Gln
Gln Ser Thr Asp Tyr Phe Arg Tyr Gln Tyr Phe Asn Pro Leu Ser 340 345
350 Thr Glu Gln Tyr Tyr His Arg Leu Asp Lys Gln Val Leu Ala Leu Val
355 360 365 Thr Gly Glu Phe Gly Ser Met Pro Asp Phe Lys Lys Ser Gly
Asp Lys 370 375 380 Ser Leu Val Asn Lys His Gln Gln Asp Lys Val Lys
Lys Ile Glu Gln 385 390 395 400 Gln Gly Glu Asn Ile Asn Thr His His
Met Lys Asn Thr Lys Glu Asp 405 410 415 Thr Gly Lys Ser Leu Ser Tyr
Lys Pro Met Ile Tyr Ile Gly Ile Val 420 425 430 Met Val Gly Phe Val
Gly Leu Ile Ser Met Ile Leu Trp Lys Arg Leu 435 440 445 His His Phe
Trp Lys 450 213096DNAStaphylococcus pseudintermedius 21gtgattacaa
ataaaaatat atatagtatt cgaaagcata aacttggcgt ggcatcattc 60ttattgggga
cattatttgt tgtagggcat gcaaataatg ctgaagcttc agaagtgagc
120gcaacaacac aagaacataa tgtcgagact gagcaaacaa aaactgaggg
cgaactaaca 180actgaggtag cacaacaagc agtcagcgaa tcagcaccta
tagctgaaaa catgcagaaa 240acaacatcag tggcaagtga aaatgcgaaa
gaggttacag cttctgatag cacacaagaa 300gtcacaaaaa ctgaagcaaa
agatacagca acaatgaaag attcagaaat tgcacaacct 360gtatcagaag
tgaataaacc tgttactcaa acagctgcac ccgtagcaga accatcaaca
420gcaaacaaac aaacttcacc acgacaagta caagaactta ctgcaccaat
ggacacaaaa 480gtaattaatg tagaaaacgg aacagatgtg acaagtaaag
tgaaagttga aaaatcgtca 540attacagggc atcagaataa agataaaaca
tatcatcaat cgaacactgt aaatccacat 600aaagctgaac gtgtgacatt
aaattatgat tggtcatttg aaaatggaat taaagctggt 660gattattttg
acttccaatt aagcgataat gtcgatacaa atggaatatc aacaataaaa
720aaagtcccac acattatgga tagtcaaaat agcgaacaaa ttattgctta
cggggaaatt 780aatgaaaaca accgtgtccg ttaccgattt atggactatg
taaatcaaaa agaaaattta 840aaaggtaaat tgtcattaaa cttatttatt
aaaccagata aagttcaaga tgaaggaaaa 900atcactgtca cttcacaatt
gggcaaggaa atgacaagtc aggaatttga cattaaatat 960attgatggtg
taaaaagccc ttcaggtatc acattaaacg gtcgtcttga tgaattatca
1020aaagcagatc aatcatttac gcattattct atatttaaac ctaagcataa
taacttaact 1080aatgtaactt taagaggcac agtttcaaat aacgcacagc
aaaatgaaaa aaatggtcaa 1140gttaatgttt acgaatatat tggtcaagga
gaattgccac aaagtgctta tgccaatgta 1200aatgatacga agcagttcaa
tgacattact aagagtatga aatcaatcaa aaataacagt 1260aatggctatg
aaattacttt tgacatgaac aaagacaatc atccttatat catagtatat
1320caaggtcact ttaacaataa tgcaaaagac tttgatttct caacaaatgc
gacaggttat 1380caaaatttaa atcaatcgga atatagttat tattggcctt
acaattattc attcaattta 1440acatgggata atggtgttgc tttctactct
aataatgcaa gtggggaagg gaacgacaaa 1500cctgtaccgc cgacttatgg
atatagtccg acagtaaata caattcaaga tactcatgcg 1560gattatcctg
taatgacttt ccaacaacct ggaactctag aggagacaga agacagtatg
1620ccaatcacta cacttaccga atctggtgag gatcgtggtg aaaatacttc
tccaattatc 1680gagacaacag aagattcaca gcctgttgag tttgaagaag
agacaaatca tggcattcaa 1740gacgtgacac ttcatgcaga tgctgttgat
tttgaggaag aaacaaacca tggtgaacaa 1800gacacggtac accactctga
tgtcgttgaa tacgacgaag atacgacaac tggcatgtta 1860acaggtgcca
tttctgacca tacaacagaa gaaggcacga tggagtacac aactgatggc
1920ttattgattg agtttgatga tgaaatgaat cctaatgtga gcggtcagta
cgatgacatc 1980acaacggata cgatagagga atcatctcat attgacacat
tcactgaact tgaatctgaa 2040tttggtcaac atgacggtat agtgacattt
gaagaagata ctatcgttga gaagccgaaa 2100acagaaaagg gtaaccgagt
accacttgta attgatttat caacaccaaa acataaccat 2160cagttcaata
ttcaacctac cgatccaaat attgatacct ctgctacgta tcgaattggc
2220aattttgtat ggcgcgatga agatcacaat ggcgtacaaa atgatggtga
acatggtctt 2280gaaggtgttc ttgtcacact taaaacagct gatggtgtcg
ttttaaatac aacgacaagt 2340gatgccaatg gacactacca gttcactaat
gttcaaaaag gaaaatatat tgttgaattc 2400actacacctg aaggttatga
agcaacaagc aaacatacta cagcgaatac tgaaaaagac 2460tctgatgggt
taatcgcaaa tatcgatgtt actcaagatg atatgtcaat cgatgctggt
2520ttcttcccgt tagaaaactg gaatcctcag ccagagccga aaaaccctga
tgatagagag 2580aaaccggcac ctgagcaacc tgatgtacct cagccagaac
cgaaaaaccc tgatgataga 2640gagaaaccgg cacctgagca acctgatgta
cctcagccag aaccgaaaaa tcctgatgat 2700agagagaaac cggcacctga
gcaacctgat gtacctcaac cagagccgaa aaatcctgat 2760gataaagaga
aaccggcacc tgagcaacct gatgtacctc aaccagagcc gaaaaatcct
2820gatgataaag agaaaccggc acctgagcaa cctgatgcac ctcaaccaaa
gccgatgctc 2880ccaggtgaaa aggtgaaacc caaaccaact catcccggtg
aagctatgca aacaacacct 2940caggacaaat caacatctca aacagatgaa
gcacttccta aaacaggtga atcatcatca 3000caatcatctg ctttaatctt
cggtggttta ctcagtctat taggacttgg tttattacgt 3060cgatcatcta
aacaaaaccg ttcttcaatg aaataa 3096221031PRTStaphylococcus
pseudintermedius 22Val Ile Thr Asn Lys Asn Ile Tyr Ser Ile Arg Lys
His Lys Leu Gly 1 5 10 15 Val Ala Ser Phe Leu Leu Gly Thr Leu Phe
Val Val Gly His Ala Asn 20 25 30 Asn Ala Glu Ala Ser Glu Val Ser
Ala Thr Thr Gln Glu His Asn Val 35 40 45 Glu Thr Glu Gln Thr Lys
Thr Glu Gly Glu Leu Thr Thr Glu Val Ala 50 55 60 Gln Gln Ala Val
Ser Glu Ser Ala Pro Ile Ala Glu Asn Met Gln Lys 65 70 75 80 Thr Thr
Ser Val Ala Ser Glu Asn Ala Lys Glu Val Thr Ala Ser Asp 85 90 95
Ser Thr Gln Glu Val Thr Lys Thr Glu Ala Lys Asp Thr Ala Thr Met 100
105 110 Lys Asp Ser Glu Ile Ala Gln Pro Val Ser Glu Val Asn Lys Pro
Val 115 120 125 Thr Gln Thr Ala Ala Pro Val Ala Glu Pro Ser Thr Ala
Asn Lys Gln 130 135 140 Thr Ser Pro Arg Gln Val Gln Glu Leu Thr Ala
Pro Met Asp Thr Lys 145 150 155 160 Val Ile Asn Val Glu Asn Gly Thr
Asp Val Thr Ser Lys Val Lys Val 165 170 175 Glu Lys Ser Ser Ile Thr
Gly His Gln Asn Lys Asp Lys Thr Tyr His 180 185 190 Gln Ser Asn Thr
Val Asn Pro His Lys Ala Glu Arg Val Thr Leu Asn 195 200 205 Tyr Asp
Trp Ser Phe Glu Asn Gly Ile Lys Ala Gly Asp Tyr Phe Asp 210 215 220
Phe Gln Leu Ser Asp Asn Val Asp Thr Asn Gly Ile Ser Thr Ile Lys 225
230 235 240 Lys Val Pro His Ile Met Asp Ser Gln Asn Ser Glu Gln Ile
Ile Ala 245 250 255 Tyr Gly Glu Ile Asn Glu Asn Asn Arg Val Arg Tyr
Arg Phe Met Asp 260 265 270 Tyr Val Asn Gln Lys Glu Asn Leu Lys Gly
Lys Leu Ser Leu Asn Leu 275 280 285 Phe Ile Lys Pro Asp Lys Val Gln
Asp Glu Gly Lys Ile Thr Val Thr 290 295 300 Ser Gln Leu Gly Lys Glu
Met Thr Ser Gln Glu Phe Asp Ile Lys Tyr 305 310 315 320 Ile Asp Gly
Val Lys Ser Pro Ser Gly Ile Thr Leu Asn Gly Arg Leu 325 330 335 Asp
Glu Leu Ser Lys Ala Asp Gln Ser Phe Thr His Tyr Ser Ile Phe 340 345
350 Lys Pro Lys His Asn Asn Leu Thr Asn Val Thr Leu Arg Gly Thr Val
355 360 365 Ser Asn Asn Ala Gln Gln Asn Glu Lys Asn Gly Gln Val Asn
Val Tyr 370 375 380 Glu Tyr Ile Gly Gln Gly Glu Leu Pro Gln Ser Ala
Tyr Ala Asn Val 385 390 395 400 Asn Asp Thr Lys Gln Phe Asn Asp Ile
Thr Lys Ser Met Lys Ser Ile 405 410 415 Lys Asn Asn Ser Asn Gly Tyr
Glu Ile Thr Phe Asp Met Asn Lys Asp 420 425 430 Asn His Pro Tyr Ile
Ile Val Tyr Gln Gly His Phe Asn Asn Asn Ala 435 440 445 Lys Asp Phe
Asp Phe Ser Thr Asn Ala Thr Gly Tyr Gln Asn Leu Asn 450 455 460 Gln
Ser Glu Tyr Ser Tyr Tyr Trp Pro Tyr Asn Tyr Ser Phe Asn Leu 465 470
475 480 Thr Trp Asp Asn Gly Val Ala Phe Tyr Ser Asn Asn Ala Ser Gly
Glu 485 490 495 Gly Asn Asp Lys Pro Val Pro Pro Thr Tyr Gly Tyr Ser
Pro Thr Val 500 505 510 Asn Thr Ile Gln Asp Thr His Ala Asp Tyr Pro
Val Met Thr Phe Gln 515 520 525 Gln Pro Gly Thr Leu Glu Glu Thr Glu
Asp Ser Met Pro Ile Thr Thr 530 535 540 Leu Thr Glu Ser Gly Glu Asp
Arg Gly Glu Asn Thr Ser Pro Ile Ile 545 550 555 560 Glu Thr Thr Glu
Asp Ser Gln Pro Val Glu Phe Glu Glu Glu Thr Asn 565 570 575 His Gly
Ile Gln Asp Val Thr Leu His Ala Asp Ala Val Asp Phe Glu 580 585 590
Glu Glu Thr Asn His Gly Glu Gln Asp Thr Val His His Ser Asp Val 595
600 605 Val Glu Tyr Asp Glu Asp Thr Thr Thr Gly Met Leu Thr Gly Ala
Ile 610 615 620 Ser Asp His Thr Thr Glu Glu Gly Thr Met Glu Tyr Thr
Thr Asp Gly 625 630 635 640 Leu Leu Ile Glu Phe Asp Asp Glu Met Asn
Pro Asn Val Ser Gly Gln 645 650 655 Tyr Asp Asp Ile Thr Thr Asp Thr
Ile Glu Glu Ser Ser His Ile Asp 660 665 670 Thr Phe Thr Glu Leu Glu
Ser Glu Phe Gly Gln His Asp Gly Ile Val 675 680 685 Thr Phe Glu Glu
Asp Thr Ile Val Glu Lys Pro Lys Thr Glu Lys Gly 690 695 700 Asn Arg
Val Pro Leu Val Ile Asp Leu Ser Thr Pro Lys His Asn His 705 710 715
720 Gln Phe Asn Ile Gln Pro Thr Asp Pro Asn Ile Asp Thr Ser Ala Thr
725 730 735 Tyr Arg Ile Gly Asn Phe Val Trp Arg Asp Glu Asp His Asn
Gly Val 740 745 750 Gln Asn Asp Gly Glu His Gly Leu Glu Gly Val Leu
Val Thr Leu Lys 755 760 765 Thr Ala Asp Gly Val Val Leu Asn Thr Thr
Thr Ser Asp Ala Asn Gly 770 775 780 His Tyr Gln Phe Thr Asn Val Gln
Lys Gly Lys Tyr Ile Val Glu Phe 785 790 795 800 Thr Thr Pro Glu Gly
Tyr Glu Ala Thr Ser Lys His Thr Thr Ala Asn 805 810 815 Thr Glu Lys
Asp Ser Asp Gly Leu Ile Ala Asn Ile Asp Val Thr Gln 820 825 830 Asp
Asp Met Ser Ile Asp Ala Gly Phe Phe Pro Leu Glu Asn Trp Asn 835 840
845
Pro Gln Pro Glu Pro Lys Asn Pro Asp Asp Arg Glu Lys Pro Ala Pro 850
855 860 Glu Gln Pro Asp Val Pro Gln Pro Glu Pro Lys Asn Pro Asp Asp
Arg 865 870 875 880 Glu Lys Pro Ala Pro Glu Gln Pro Asp Val Pro Gln
Pro Glu Pro Lys 885 890 895 Asn Pro Asp Asp Arg Glu Lys Pro Ala Pro
Glu Gln Pro Asp Val Pro 900 905 910 Gln Pro Glu Pro Lys Asn Pro Asp
Asp Lys Glu Lys Pro Ala Pro Glu 915 920 925 Gln Pro Asp Val Pro Gln
Pro Glu Pro Lys Asn Pro Asp Asp Lys Glu 930 935 940 Lys Pro Ala Pro
Glu Gln Pro Asp Ala Pro Gln Pro Lys Pro Met Leu 945 950 955 960 Pro
Gly Glu Lys Val Lys Pro Lys Pro Thr His Pro Gly Glu Ala Met 965 970
975 Gln Thr Thr Pro Gln Asp Lys Ser Thr Ser Gln Thr Asp Glu Ala Leu
980 985 990 Pro Lys Thr Gly Glu Ser Ser Ser Gln Ser Ser Ala Leu Ile
Phe Gly 995 1000 1005 Gly Leu Leu Ser Leu Leu Gly Leu Gly Leu Leu
Arg Arg Ser Ser 1010 1015 1020 Lys Gln Asn Arg Ser Ser Met Lys 1025
1030 231704DNAStaphylococcus pseudintermedius 23atggcatttg
atggtatgtt tacaagaaaa atggtagaag atttacaatt tctcgtttct 60gggcgtattc
ataaaatcaa tcaaccggaa aacgatacaa tcatcatggt tataagacag
120caacgccaaa atcatcaatt gttgttgtcg attcacccga attttgcacg
gattcacctc 180actacaaaaa aatatgataa tccatttgaa ccgccgatgt
ttgcgcgcgt ctttcgtaaa 240catttagaag gtggacgtat ccttgccatt
cgccaaatcg gaaatgaccg tcgcatcgaa 300atggacgtgg aaagtaaaga
tgaaattggt gacacgattc atcgtacagt gattttagaa 360attatgggca
aacatagtaa tctcattctc gttaatgaag aacgtaaaat tttagaaggt
420tttaaacacc ttacaccaaa tacgaatcaa tttagaaccg tgatgccagg
ttttcaatat 480gaagtgccgc caacacaaca taaacagaac ccttatgcat
atactggtgc gcaagtgctc 540caacatattg atttcaatgc gggcaaaatt
gatcgccaac tgcttcaaac gtttgaaggt 600ttttcaccgt taatcacaaa
agaaatcaca tcaagacgcc attttatgac cacacaaact 660ttacctgaag
cttttgacga agtgatggcc gaaacgaaag cgacacccca accggtattt
720cataaaaata acgaaacagg taaagaagac ttttatttta tgaagttaca
tcagttttac 780gatgattgcg tcacatatga ttcactccat gaactgctcg
accgttttta tgatgcacgc 840ggtgaacgtg aacgcgtcaa acaacgtgca
aacgatttag tcaaactcgt ccaacaatta 900cttcaaaaat atcaaaataa
attaagtaag ctcgtcgatg aacaagcggg gactgaagaa 960aaagaaaatc
aacaattgta cggcgagtta atcacagcga atatttatca actcaaacct
1020ggagatcgcc agttagaaac agtaaattat tatacaggag aaaacgtgac
tattccgtta 1080aatccacaaa agtcacctgc tgaaaatgcg caatactatt
acaagcaata caaccgaatg 1140aaaacacgtg agcgcgaatt gacccatcaa
attactttaa cggaagaaaa tatcgcttat 1200tttgaaaata tcgagcaaca
gttgtcacac attcaagttc atgaaattga cgatattcgt 1260gaagaactag
cagaacaagg ctttatcaaa caaaagaaac agcagaaaaa gaaaaagcaa
1320caaaaaatcc agttacaatc ctacgtttcg actgatggcg atacgatttt
agtcggtaaa 1380aataataagc aaaatgatta tttaacgaat aaacgtgcgc
aaaaatcgca tttatggttc 1440catacaaaag atatcccagg aagccatgtc
gtgattttaa atgatgcgcc aagtgacaaa 1500acgattgaag aagcggcgat
gattgcagcg tacttttcaa aggcggggca atcgggacaa 1560attccagtgg
attatacaac aattcgcaat gtgcataagc cgagtggcag taaacctgga
1620tttgtaacgt acgataacca gaagacgctt tacgcaacgc cggattatga
catgattcgt 1680cgattgaaag ctgaagaagc gtaa
170424567PRTStaphylococcus pseudintermedius 24Met Ala Phe Asp Gly
Met Phe Thr Arg Lys Met Val Glu Asp Leu Gln 1 5 10 15 Phe Leu Val
Ser Gly Arg Ile His Lys Ile Asn Gln Pro Glu Asn Asp 20 25 30 Thr
Ile Ile Met Val Ile Arg Gln Gln Arg Gln Asn His Gln Leu Leu 35 40
45 Leu Ser Ile His Pro Asn Phe Ala Arg Ile His Leu Thr Thr Lys Lys
50 55 60 Tyr Asp Asn Pro Phe Glu Pro Pro Met Phe Ala Arg Val Phe
Arg Lys 65 70 75 80 His Leu Glu Gly Gly Arg Ile Leu Ala Ile Arg Gln
Ile Gly Asn Asp 85 90 95 Arg Arg Ile Glu Met Asp Val Glu Ser Lys
Asp Glu Ile Gly Asp Thr 100 105 110 Ile His Arg Thr Val Ile Leu Glu
Ile Met Gly Lys His Ser Asn Leu 115 120 125 Ile Leu Val Asn Glu Glu
Arg Lys Ile Leu Glu Gly Phe Lys His Leu 130 135 140 Thr Pro Asn Thr
Asn Gln Phe Arg Thr Val Met Pro Gly Phe Gln Tyr 145 150 155 160 Glu
Val Pro Pro Thr Gln His Lys Gln Asn Pro Tyr Ala Tyr Thr Gly 165 170
175 Ala Gln Val Leu Gln His Ile Asp Phe Asn Ala Gly Lys Ile Asp Arg
180 185 190 Gln Leu Leu Gln Thr Phe Glu Gly Phe Ser Pro Leu Ile Thr
Lys Glu 195 200 205 Ile Thr Ser Arg Arg His Phe Met Thr Thr Gln Thr
Leu Pro Glu Ala 210 215 220 Phe Asp Glu Val Met Ala Glu Thr Lys Ala
Thr Pro Gln Pro Val Phe 225 230 235 240 His Lys Asn Asn Glu Thr Gly
Lys Glu Asp Phe Tyr Phe Met Lys Leu 245 250 255 His Gln Phe Tyr Asp
Asp Cys Val Thr Tyr Asp Ser Leu His Glu Leu 260 265 270 Leu Asp Arg
Phe Tyr Asp Ala Arg Gly Glu Arg Glu Arg Val Lys Gln 275 280 285 Arg
Ala Asn Asp Leu Val Lys Leu Val Gln Gln Leu Leu Gln Lys Tyr 290 295
300 Gln Asn Lys Leu Ser Lys Leu Val Asp Glu Gln Ala Gly Thr Glu Glu
305 310 315 320 Lys Glu Asn Gln Gln Leu Tyr Gly Glu Leu Ile Thr Ala
Asn Ile Tyr 325 330 335 Gln Leu Lys Pro Gly Asp Arg Gln Leu Glu Thr
Val Asn Tyr Tyr Thr 340 345 350 Gly Glu Asn Val Thr Ile Pro Leu Asn
Pro Gln Lys Ser Pro Ala Glu 355 360 365 Asn Ala Gln Tyr Tyr Tyr Lys
Gln Tyr Asn Arg Met Lys Thr Arg Glu 370 375 380 Arg Glu Leu Thr His
Gln Ile Thr Leu Thr Glu Glu Asn Ile Ala Tyr 385 390 395 400 Phe Glu
Asn Ile Glu Gln Gln Leu Ser His Ile Gln Val His Glu Ile 405 410 415
Asp Asp Ile Arg Glu Glu Leu Ala Glu Gln Gly Phe Ile Lys Gln Lys 420
425 430 Lys Gln Gln Lys Lys Lys Lys Gln Gln Lys Ile Gln Leu Gln Ser
Tyr 435 440 445 Val Ser Thr Asp Gly Asp Thr Ile Leu Val Gly Lys Asn
Asn Lys Gln 450 455 460 Asn Asp Tyr Leu Thr Asn Lys Arg Ala Gln Lys
Ser His Leu Trp Phe 465 470 475 480 His Thr Lys Asp Ile Pro Gly Ser
His Val Val Ile Leu Asn Asp Ala 485 490 495 Pro Ser Asp Lys Thr Ile
Glu Glu Ala Ala Met Ile Ala Ala Tyr Phe 500 505 510 Ser Lys Ala Gly
Gln Ser Gly Gln Ile Pro Val Asp Tyr Thr Thr Ile 515 520 525 Arg Asn
Val His Lys Pro Ser Gly Ser Lys Pro Gly Phe Val Thr Tyr 530 535 540
Asp Asn Gln Lys Thr Leu Tyr Ala Thr Pro Asp Tyr Asp Met Ile Arg 545
550 555 560 Arg Leu Lys Ala Glu Glu Ala 565 254167DNAStaphylococcus
pseudintermedius 25atggtcaaaa aatttggtta taaaacacct acaatcgttg
cacttacttt ggctggaact 60gcattttctg cacaccaagc caatgccgct gaacaagttg
cacctgaaaa aacacctacg 120aatgtacttg atgatcaata cgcattaaaa
caagctgatg atgcgaaaca aacgacacaa 180ggaacaacac ttgcaggttc
aaaagaatac aaggatcctt cacaaattga tacgactcaa 240gtcgatacag
cagcacaaac tgaaacgccc gtagaaggag ggcaacaaga cgcacaacaa
300cctactacaa ctgatgaagc gacatcaaca gatcatactg tatcaaaagg
tacaaacgaa 360agtgcatcac ctgcaacagc ttctatagat gaaggaacat
taaacgcaca agtcaattca 420gatgaaacgg ctactaaccg tacacaagac
gtcactgaaa atgtgacaaa atatccttat 480cattcaagtg aaatcgatac
acatgaagac gcaactgtgt caccagatac atatcatgca 540ctggacacgc
atgcgcaaca accttcagca atggatgtaa gcgattcaac atcagcacaa
600actgaagcga cgcaagtaaa tacgtcaaca aatgtaaatg acaaagaggc
cgtttcgaca 660acagaagatg cacctactac acaacttcaa gcagctgtac
aatctgaagc caacaaagaa 720gcgaaggcaa ctactgaaac agctcaaaat
aaaacacctc aagttgaaaa gaaagcaaca 780gcaactcaaa atacagcaca
gttagcaacg gggcatcagg atattactga caaagtctca 840aaacgcgtag
cagtgacaaa tgaaacgaaa gcggatgcca caacagcgaa aacacaagca
900cctacttcag tgacacatca agctgataca caagcaaaaa cgataacaga
caagaaggca 960acaacttaca gtgcacaaac cgcaactgac caagacataa
atgcgaatcc ggacggtcca 1020acacctccac gcgttggcgg taaagggggt
ccccctgctt cactttcact ccaatcgact 1080ggtcaaacag cattccgttc
agctgtcgct agtaaaccga gtgcatatca acctaaagtg 1140aaatcgtcta
ttaatgacta tattcgtaag caaaactaca aagtgcctgt atatgaagaa
1200gattattcaa gttacttccc taaatacggt tatcgtaatg gtgtcggtaa
acctgagggc 1260atcatcgtgc atgatacagc aaatgacaac tctacaattg
atggcgaaat cagttacatg 1320aaaagaaatt atcaaaatgc tttcgtacat
ggctttatta atggtcaacg tattgttgaa 1380acgcaaccta cagattattt
agcatggggt gcaggtgcga ttgcgaatga acgctttatt 1440catatcgaac
tcgttcatgt tcacagtaaa gaagatttcg cacgtcaaat gaacaatatg
1500gcagattatg cggcgacgaa cttacaatat tatggccttt ctccagatag
tgcggaatat 1560gatggtcgtg ggacagtttg gacacatgat gctgtttcta
gatttttagg tggtacagac 1620cataccgatc cgcacggcta tttaaaacaa
catggttatt cctttgatgc gttgtatgat 1680ttaatcaatg aaaaatatca
agtgaaaatg ggttatgcct cacctgctaa ctcgtcttca 1740aaaccatcaa
caaatactgg cttaacagtt aaaaacacaa caggtttcgg ccgtattaac
1800acaacaaata gcggtttata tacgaccgtt tatgatcaaa aaggtaaagc
gacgaatcaa 1860acgaatcaaa cgttaaaagt tacaaaagaa gcgacgttaa
atggcaacaa attctattta 1920atgagtgatg caaaatctaa tcaaacactc
ggttgggtca aatcaaacga cgcaacatat 1980caagctgccc aagctgagaa
aaaagtaacg aaaacgtata ctgtcaaacc aggaacaaca 2040gtatatcaag
tgccttgggg tgcctcatct caaacagtag gcaaagctcc aggtacgtca
2100aaccaatcat tcaaatcaac gaaagaacaa actgttgcga aaacgaaatg
gctttatggg 2160acagttggca aagtgacagg ctggattaat gcaagtagtg
ttgtagcaaa tgatcaaaaa 2220ccatcgacga ataccgcact aaaagtaaca
actgacactg gtctcggtcg cattaaagac 2280aaaaatagtg gtttatacgc
aacggtatat gataaaactg gtaaaagcac ttcagccact 2340aaccaaacat
taaaagtaac gaaaaaagca agtgtcaatg gccaatcatt ctatttagta
2400tcagattatg ctaaaggtac aaatgttggt tgggtgaaac agtcagatgt
cgaatatcaa 2460acaagtaaag ccccttctaa agtgaatcaa aattatacga
ttaaatcggg tgcgaaattg 2520tatcaagtgc cttggggtac aagtaaacaa
gttgccggta cagtgacagg tgctgcgaca 2580caaacattta aggcaacaca
atctcaaact gtaggtaaag caacatactt gtatgggaca 2640gttggcaaat
tatctggttg gattaattca acagcattag cagctcaaaa aacaacaacg
2700aatgttacta aaacaatttc tcaaatcggt caactgaaca cgaaaaatag
cggtgtcaaa 2760gcttctattt atgacaaaac agcaaaagat gcatccaaat
gggcaggtca aacttataaa 2820attactaaaa cagcttctgc caataacgaa
gactatgtat tactgcaaaa tagtacagga 2880ggcacgccac tcggttggtt
caatgttaaa gacgtcacaa cacgcaactt aggtgctgaa 2940acagctgtta
aagggcggta cactgttaat agtaaaacat ctggactcta cgctatgcct
3000tggggtacaa cgaagcaacg tgtcgataca ttaaaaaatg ccacaagtcg
tttatttaca 3060gcttcaaaat cagttaaagt cggtaatgat acattcttat
tcggtacagt gaatcaaaaa 3120ttgggctgga ttaatcaaaa agacttaaca
gctgtagcag caaaagttgc aaacatgaaa 3180actgcatcga atagcgcagt
caaaggtgcc gcaatcacaa ctttgaaaaa agtagaagat 3240tatgtgatta
cgaataaaaa tggttattat tacactaaag ttggagattc aaaaacagct
3300ggtgctttaa aaggttttta tcaacaaatt tttaaagtcg aaaaaacatc
tttactgaac 3360ggcattactt ggtactatgg cgcattccaa aacgggacga
aaggatggat taaagcagct 3420gacatacgtt catcattcat tcaacatact
gcggtcagta gcacattgaa agcagcactc 3480gataaacaaa tggcgctgac
ttacccgcct caagttcaac gtgtagccgg taaatgggtc 3540aatgcgaatc
gtgcagaaac tgaaaaagca atgaataccg cagcaattga aaaagatccg
3600actctcattt accaattttt aaaacttgat aaataccaag gtcttggcgt
agaagaactt 3660aataaattgt taagaggcaa aggcatttta gaaggtcaag
gtgccgcatt taaagaagcc 3720gcacaaaaac acaatattaa tgaggtttac
ttaatgtctc acgcattttt agaaacaggt 3780aacgggactt ctcaattagc
caatggcggt cacgtagata aaaataataa agtcgtaaca 3840aacggtaaac
cgaagtatta caacatgttc ggtatcgggg caattgatac agacgcttta
3900cgcaatggct ttaaaactgc tgaaaaatat ggttggaata cggtcagcaa
agcgattatc 3960ggtggcgcaa aattcatccg tgatcagtac atcggttcag
gacaaaacac attgtatcgt 4020atgcgttgga atccagaaca ccctgccaca
catcagtatg cgactgatat taattgggca 4080aatgtaaacg cacaacgcat
gaaatatttc tatgatcaaa ttggtgaaac aggtaaatat 4140ttcgacgtcg
atgtatataa gaagtag 4167261388PRTStaphylococcus pseudintermedius
26Met Val Lys Lys Phe Gly Tyr Lys Thr Pro Thr Ile Val Ala Leu Thr 1
5 10 15 Leu Ala Gly Thr Ala Phe Ser Ala His Gln Ala Asn Ala Ala Glu
Gln 20 25 30 Val Ala Pro Glu Lys Thr Pro Thr Asn Val Leu Asp Asp
Gln Tyr Ala 35 40 45 Leu Lys Gln Ala Asp Asp Ala Lys Gln Thr Thr
Gln Gly Thr Thr Leu 50 55 60 Ala Gly Ser Lys Glu Tyr Lys Asp Pro
Ser Gln Ile Asp Thr Thr Gln 65 70 75 80 Val Asp Thr Ala Ala Gln Thr
Glu Thr Pro Val Glu Gly Gly Gln Gln 85 90 95 Asp Ala Gln Gln Pro
Thr Thr Thr Asp Glu Ala Thr Ser Thr Asp His 100 105 110 Thr Val Ser
Lys Gly Thr Asn Glu Ser Ala Ser Pro Ala Thr Ala Ser 115 120 125 Ile
Asp Glu Gly Thr Leu Asn Ala Gln Val Asn Ser Asp Glu Thr Ala 130 135
140 Thr Asn Arg Thr Gln Asp Val Thr Glu Asn Val Thr Lys Tyr Pro Tyr
145 150 155 160 His Ser Ser Glu Ile Asp Thr His Glu Asp Ala Thr Val
Ser Pro Asp 165 170 175 Thr Tyr His Ala Leu Asp Thr His Ala Gln Gln
Pro Ser Ala Met Asp 180 185 190 Val Ser Asp Ser Thr Ser Ala Gln Thr
Glu Ala Thr Gln Val Asn Thr 195 200 205 Ser Thr Asn Val Asn Asp Lys
Glu Ala Val Ser Thr Thr Glu Asp Ala 210 215 220 Pro Thr Thr Gln Leu
Gln Ala Ala Val Gln Ser Glu Ala Asn Lys Glu 225 230 235 240 Ala Lys
Ala Thr Thr Glu Thr Ala Gln Asn Lys Thr Pro Gln Val Glu 245 250 255
Lys Lys Ala Thr Ala Thr Gln Asn Thr Ala Gln Leu Ala Thr Gly His 260
265 270 Gln Asp Ile Thr Asp Lys Val Ser Lys Arg Val Ala Val Thr Asn
Glu 275 280 285 Thr Lys Ala Asp Ala Thr Thr Ala Lys Thr Gln Ala Pro
Thr Ser Val 290 295 300 Thr His Gln Ala Asp Thr Gln Ala Lys Thr Ile
Thr Asp Lys Lys Ala 305 310 315 320 Thr Thr Tyr Ser Ala Gln Thr Ala
Thr Asp Gln Asp Ile Asn Ala Asn 325 330 335 Pro Asp Gly Pro Thr Pro
Pro Arg Val Gly Gly Lys Gly Gly Pro Pro 340 345 350 Ala Ser Leu Ser
Leu Gln Ser Thr Gly Gln Thr Ala Phe Arg Ser Ala 355 360 365 Val Ala
Ser Lys Pro Ser Ala Tyr Gln Pro Lys Val Lys Ser Ser Ile 370 375 380
Asn Asp Tyr Ile Arg Lys Gln Asn Tyr Lys Val Pro Val Tyr Glu Glu 385
390 395 400 Asp Tyr Ser Ser Tyr Phe Pro Lys Tyr Gly Tyr Arg Asn Gly
Val Gly 405 410 415 Lys Pro Glu Gly Ile Ile Val His Asp Thr Ala Asn
Asp Asn Ser Thr 420 425 430 Ile Asp Gly Glu Ile Ser Tyr Met Lys Arg
Asn Tyr Gln Asn Ala Phe 435 440 445 Val His Gly Phe Ile Asn Gly Gln
Arg Ile Val Glu Thr Gln Pro Thr 450 455 460 Asp Tyr Leu Ala Trp Gly
Ala Gly Ala Ile Ala Asn Glu Arg Phe Ile 465 470 475 480 His Ile Glu
Leu Val His Val His Ser Lys Glu Asp Phe Ala Arg Gln 485 490 495 Met
Asn Asn Met Ala Asp Tyr Ala Ala Thr Asn Leu Gln Tyr Tyr Gly 500 505
510 Leu Ser Pro Asp Ser Ala Glu Tyr Asp Gly Arg Gly Thr Val Trp Thr
515 520 525 His Asp Ala Val Ser Arg Phe Leu Gly Gly Thr Asp His Thr
Asp Pro 530 535 540 His Gly Tyr Leu Lys Gln His Gly Tyr Ser Phe Asp
Ala Leu Tyr Asp 545 550 555 560 Leu Ile Asn Glu Lys Tyr Gln Val Lys
Met Gly Tyr Ala Ser Pro Ala 565 570 575 Asn Ser Ser Ser Lys Pro Ser
Thr Asn Thr Gly Leu Thr Val Lys Asn 580 585 590 Thr Thr Gly Phe Gly
Arg Ile Asn Thr Thr Asn Ser Gly Leu Tyr Thr 595 600 605 Thr Val Tyr
Asp Gln Lys Gly Lys Ala Thr Asn Gln Thr Asn Gln Thr 610 615 620
Leu Lys Val Thr Lys Glu Ala Thr Leu Asn Gly Asn Lys Phe Tyr Leu 625
630 635 640 Met Ser Asp Ala Lys Ser Asn Gln Thr Leu Gly Trp Val Lys
Ser Asn 645 650 655 Asp Ala Thr Tyr Gln Ala Ala Gln Ala Glu Lys Lys
Val Thr Lys Thr 660 665 670 Tyr Thr Val Lys Pro Gly Thr Thr Val Tyr
Gln Val Pro Trp Gly Ala 675 680 685 Ser Ser Gln Thr Val Gly Lys Ala
Pro Gly Thr Ser Asn Gln Ser Phe 690 695 700 Lys Ser Thr Lys Glu Gln
Thr Val Ala Lys Thr Lys Trp Leu Tyr Gly 705 710 715 720 Thr Val Gly
Lys Val Thr Gly Trp Ile Asn Ala Ser Ser Val Val Ala 725 730 735 Asn
Asp Gln Lys Pro Ser Thr Asn Thr Ala Leu Lys Val Thr Thr Asp 740 745
750 Thr Gly Leu Gly Arg Ile Lys Asp Lys Asn Ser Gly Leu Tyr Ala Thr
755 760 765 Val Tyr Asp Lys Thr Gly Lys Ser Thr Ser Ala Thr Asn Gln
Thr Leu 770 775 780 Lys Val Thr Lys Lys Ala Ser Val Asn Gly Gln Ser
Phe Tyr Leu Val 785 790 795 800 Ser Asp Tyr Ala Lys Gly Thr Asn Val
Gly Trp Val Lys Gln Ser Asp 805 810 815 Val Glu Tyr Gln Thr Ser Lys
Ala Pro Ser Lys Val Asn Gln Asn Tyr 820 825 830 Thr Ile Lys Ser Gly
Ala Lys Leu Tyr Gln Val Pro Trp Gly Thr Ser 835 840 845 Lys Gln Val
Ala Gly Thr Val Thr Gly Ala Ala Thr Gln Thr Phe Lys 850 855 860 Ala
Thr Gln Ser Gln Thr Val Gly Lys Ala Thr Tyr Leu Tyr Gly Thr 865 870
875 880 Val Gly Lys Leu Ser Gly Trp Ile Asn Ser Thr Ala Leu Ala Ala
Gln 885 890 895 Lys Thr Thr Thr Asn Val Thr Lys Thr Ile Ser Gln Ile
Gly Gln Leu 900 905 910 Asn Thr Lys Asn Ser Gly Val Lys Ala Ser Ile
Tyr Asp Lys Thr Ala 915 920 925 Lys Asp Ala Ser Lys Trp Ala Gly Gln
Thr Tyr Lys Ile Thr Lys Thr 930 935 940 Ala Ser Ala Asn Asn Glu Asp
Tyr Val Leu Leu Gln Asn Ser Thr Gly 945 950 955 960 Gly Thr Pro Leu
Gly Trp Phe Asn Val Lys Asp Val Thr Thr Arg Asn 965 970 975 Leu Gly
Ala Glu Thr Ala Val Lys Gly Arg Tyr Thr Val Asn Ser Lys 980 985 990
Thr Ser Gly Leu Tyr Ala Met Pro Trp Gly Thr Thr Lys Gln Arg Val 995
1000 1005 Asp Thr Leu Lys Asn Ala Thr Ser Arg Leu Phe Thr Ala Ser
Lys 1010 1015 1020 Ser Val Lys Val Gly Asn Asp Thr Phe Leu Phe Gly
Thr Val Asn 1025 1030 1035 Gln Lys Leu Gly Trp Ile Asn Gln Lys Asp
Leu Thr Ala Val Ala 1040 1045 1050 Ala Lys Val Ala Asn Met Lys Thr
Ala Ser Asn Ser Ala Val Lys 1055 1060 1065 Gly Ala Ala Ile Thr Thr
Leu Lys Lys Val Glu Asp Tyr Val Ile 1070 1075 1080 Thr Asn Lys Asn
Gly Tyr Tyr Tyr Thr Lys Val Gly Asp Ser Lys 1085 1090 1095 Thr Ala
Gly Ala Leu Lys Gly Phe Tyr Gln Gln Ile Phe Lys Val 1100 1105 1110
Glu Lys Thr Ser Leu Leu Asn Gly Ile Thr Trp Tyr Tyr Gly Ala 1115
1120 1125 Phe Gln Asn Gly Thr Lys Gly Trp Ile Lys Ala Ala Asp Ile
Arg 1130 1135 1140 Ser Ser Phe Ile Gln His Thr Ala Val Ser Ser Thr
Leu Lys Ala 1145 1150 1155 Ala Leu Asp Lys Gln Met Ala Leu Thr Tyr
Pro Pro Gln Val Gln 1160 1165 1170 Arg Val Ala Gly Lys Trp Val Asn
Ala Asn Arg Ala Glu Thr Glu 1175 1180 1185 Lys Ala Met Asn Thr Ala
Ala Ile Glu Lys Asp Pro Thr Leu Ile 1190 1195 1200 Tyr Gln Phe Leu
Lys Leu Asp Lys Tyr Gln Gly Leu Gly Val Glu 1205 1210 1215 Glu Leu
Asn Lys Leu Leu Arg Gly Lys Gly Ile Leu Glu Gly Gln 1220 1225 1230
Gly Ala Ala Phe Lys Glu Ala Ala Gln Lys His Asn Ile Asn Glu 1235
1240 1245 Val Tyr Leu Met Ser His Ala Phe Leu Glu Thr Gly Asn Gly
Thr 1250 1255 1260 Ser Gln Leu Ala Asn Gly Gly His Val Asp Lys Asn
Asn Lys Val 1265 1270 1275 Val Thr Asn Gly Lys Pro Lys Tyr Tyr Asn
Met Phe Gly Ile Gly 1280 1285 1290 Ala Ile Asp Thr Asp Ala Leu Arg
Asn Gly Phe Lys Thr Ala Glu 1295 1300 1305 Lys Tyr Gly Trp Asn Thr
Val Ser Lys Ala Ile Ile Gly Gly Ala 1310 1315 1320 Lys Phe Ile Arg
Asp Gln Tyr Ile Gly Ser Gly Gln Asn Thr Leu 1325 1330 1335 Tyr Arg
Met Arg Trp Asn Pro Glu His Pro Ala Thr His Gln Tyr 1340 1345 1350
Ala Thr Asp Ile Asn Trp Ala Asn Val Asn Ala Gln Arg Met Lys 1355
1360 1365 Tyr Phe Tyr Asp Gln Ile Gly Glu Thr Gly Lys Tyr Phe Asp
Val 1370 1375 1380 Asp Val Tyr Lys Lys 1385 273435DNAStaphylococcus
pseudintermedius 27gtgtcgacag aaaaacaaga tgatacacaa gcaaaagcga
atgcactttc tacagatgat 60tcaacaccta caacagaaca atcaaaaagt gataccgaac
caacgcaaaa tcaagaagtg 120aatgaaaaag aagcaacaca agttgagcaa
actccagata atgcatcatc agaatttaaa 180gacagtgcag cacaagatga
aacaacatcg aaagacgctg acattgctca aacaaaagaa 240gcaaaaaatg
aagcattgca aagtgactca tcagcaaacc tatcaaatca agaagcagaa
300aaagaaaaca caactaacag tgaatctcaa gtaaatgaac aacctaaagc
agatacaact 360tctgattcac aagtttcaaa tacacctcaa caagatccta
catcgacagt accttcacca 420gaaacatcag aagacaatcg accttcaaca
gaattaaaaa atagtgaaac aactgcttct 480caaacaactt taaacgaaca
acctactgaa tcaacatcca atcaaactga aacgacaaaa 540gcaccaacaa
atacaacagt cgcaaacaaa aaagcacctg cacaattaaa agacattaaa
600ggtacaactc aacttcgcgc agtcagtgca agtcaaccta ctgctgttgc
agctggtggg 660acaaacgtaa atgacaaagt aacagcatca aatatgaaaa
taactgaatc ttatatcgag 720ccaaacaact caggaaactt ttatttaaaa
agtaacttta acgtaaacgg gactgttaaa 780gaaggtgact actttactgt
aaaaatgcct gacactgtca atacttttgg tgacacgcgc 840cattcacctg
actttagaga aaaaattaca aatcaaaaag gtgaagttgt ggctttaggt
900gaatatgatg ttgccaacca tactatgaca tacacgttca ctaatgtcgt
taataattta 960gaaaatgtgt ccggttcgtt taacttgact caatttatgg
atcgtaaagt ggcaacagat 1020tctcaaacat atccattaaa atacgacatt
gcaggcgaat ctttagatac acaaattaaa 1080gtgaattacg gtcaatatta
cagtgaaggt gattctaact taaaatcaat gatcacttca 1140gaagatccta
aaactgggga atatgatcaa tacatttatg tcaacccatt acaaaaaacg
1200gcaaacggta cagttgtaag agttcaaggg ttccaagttg atccaactaa
gagtaatggg 1260caagtgaaac cagatacaac gcagatcaag attttaaaag
ttgctgatgg tcaaccactt 1320aatagtagtt tcggtgtgaa tgacagtgaa
tatgaagatg tcacaaaaca atttaatatt 1380gtttatcgtg ataataattt
ggcagatatt tactttggaa acttaaatgg gcaacgctat 1440atcgttaaag
tgacgagcaa agaaaatttg gattctaaag aggatttaaa cttgcgtgct
1500attatggcca ctcaaaaccg atatggtcaa tataactata ttacttggga
taacgatatt 1560gtgaaaagct cttctggtgg tacagccgac ggaaatgaag
catcatatca attaggcgac 1620aaagtttgga atgatgtgaa taaaaatggt
atccaagatc aaggtgaaac tggtattgct 1680gatgtaaagg ttactttaaa
agatcttgat ggcaacattt tggatacaac ttatacaaac 1740acgaatggta
aatatatctt tgataattta aaaaatggta attatcaagt gggttttgaa
1800acaccggaag gctatgctgc aagtccatcc aaccaaggta atgacgccct
tgactctgat 1860ggtcctacaa atgtacaagc tgtcattagt gatgggaaca
acttaactat cgaccaaggt 1920ttttaccaaa ctgaaacacc aacacacaac
gtcggcgaca aagtttggga agacttaaat 1980aaagatggca tccaagacca
aaatgaacca ggtatcgcta acgttaaggt cactttaaaa 2040gacgcggatg
gtaacgttgt ggatacacgt acgactgatg ataaagggaa ttacttattc
2100gaaaaagtta aagaaggcga atatacaatt gaatttgaaa cgcctgaagg
ttatacaccg 2160acacaaacag gccaaggcag agtcagcact gactctaatg
ggacatcttc ccttatttta 2220gtcgaaggta acgatgactt aacaatcgat
agcggtttct acaaagaacc tgttacacac 2280aaagttggcg acaaagtttg
ggatgactta aataaagacg gtatccaaga tgacaatgaa 2340ccaggcatct
ctgacgttaa agtcacttta aaagatgcgg atggtaacgt cgtagataca
2400cgtacaactg atgctaacgg taactattta tttgaaaacg tgaaagaagg
cgactatacg 2460attgaatttg aaacgcctga aggttacaca ccgactgtta
caggtcaagg tacagctgat 2520aatgactcta acggtacatc tacaaaagtt
acagttaaag atggcgatga cttaacaatt 2580gacagtggtt tcactcaagt
tacacctgag ccaccgacac ataatgttgg cgacaaagtt 2640tgggatgact
taaataaaga cggtatccaa gatgacaatg aaccaggcat ctctgacgtt
2700aaagtcactt taaaagatgc ggatggtaac gtcgtagata cacgtacaac
tgatgctaac 2760ggtaactatt tatttgaaaa cgtgaaagaa ggcgactata
cgattgaatt tgaaacgcct 2820gaaggttaca caccgactgt tacaggtcaa
ggtacagctg ataatgactc taacggtaca 2880tctacaaaag ttacagttaa
agatggcgat gacttaacaa ttgacagtgg tttcactcaa 2940gttacacctg
agccaccgac tgaacctgaa aaccctagtc cagagcaacc ttctgaaccg
3000ggtcaacctg aaaatcctag tccagagcaa ccttctgaac caggtcaacc
tgaaaatcct 3060agtccagagc aaccttctga accaggtcaa cctgaaaatc
ctagtccaga acaaccttct 3120gaaccgggtc aacctgaaaa tcctagtcca
gaacagcctt ctgagccagg acaacctaaa 3180aatcctagtc cagaacagcc
aaataatcca agtgtgccag gtgttcaaaa tcctgaaaaa 3240ccaagcttaa
ctccagtcac acaaccggtt cattcaaacg gcaataaagc aaaaccatct
3300caacaacaaa aagctttacc tgaaacaggt gaaactgaat cacatcaagg
tacattattc 3360ggtggtattt tagctgcttt aggcgcatta ctctttgcac
gtaaaaaacg ccacgataaa 3420aaacaatcac actaa
3435281144PRTStaphylococcus pseudintermedius 28Val Ser Thr Glu Lys
Gln Asp Asp Thr Gln Ala Lys Ala Asn Ala Leu 1 5 10 15 Ser Thr Asp
Asp Ser Thr Pro Thr Thr Glu Gln Ser Lys Ser Asp Thr 20 25 30 Glu
Pro Thr Gln Asn Gln Glu Val Asn Glu Lys Glu Ala Thr Gln Val 35 40
45 Glu Gln Thr Pro Asp Asn Ala Ser Ser Glu Phe Lys Asp Ser Ala Ala
50 55 60 Gln Asp Glu Thr Thr Ser Lys Asp Ala Asp Ile Ala Gln Thr
Lys Glu 65 70 75 80 Ala Lys Asn Glu Ala Leu Gln Ser Asp Ser Ser Ala
Asn Leu Ser Asn 85 90 95 Gln Glu Ala Glu Lys Glu Asn Thr Thr Asn
Ser Glu Ser Gln Val Asn 100 105 110 Glu Gln Pro Lys Ala Asp Thr Thr
Ser Asp Ser Gln Val Ser Asn Thr 115 120 125 Pro Gln Gln Asp Pro Thr
Ser Thr Val Pro Ser Pro Glu Thr Ser Glu 130 135 140 Asp Asn Arg Pro
Ser Thr Glu Leu Lys Asn Ser Glu Thr Thr Ala Ser 145 150 155 160 Gln
Thr Thr Leu Asn Glu Gln Pro Thr Glu Ser Thr Ser Asn Gln Thr 165 170
175 Glu Thr Thr Lys Ala Pro Thr Asn Thr Thr Val Ala Asn Lys Lys Ala
180 185 190 Pro Ala Gln Leu Lys Asp Ile Lys Gly Thr Thr Gln Leu Arg
Ala Val 195 200 205 Ser Ala Ser Gln Pro Thr Ala Val Ala Ala Gly Gly
Thr Asn Val Asn 210 215 220 Asp Lys Val Thr Ala Ser Asn Met Lys Ile
Thr Glu Ser Tyr Ile Glu 225 230 235 240 Pro Asn Asn Ser Gly Asn Phe
Tyr Leu Lys Ser Asn Phe Asn Val Asn 245 250 255 Gly Thr Val Lys Glu
Gly Asp Tyr Phe Thr Val Lys Met Pro Asp Thr 260 265 270 Val Asn Thr
Phe Gly Asp Thr Arg His Ser Pro Asp Phe Arg Glu Lys 275 280 285 Ile
Thr Asn Gln Lys Gly Glu Val Val Ala Leu Gly Glu Tyr Asp Val 290 295
300 Ala Asn His Thr Met Thr Tyr Thr Phe Thr Asn Val Val Asn Asn Leu
305 310 315 320 Glu Asn Val Ser Gly Ser Phe Asn Leu Thr Gln Phe Met
Asp Arg Lys 325 330 335 Val Ala Thr Asp Ser Gln Thr Tyr Pro Leu Lys
Tyr Asp Ile Ala Gly 340 345 350 Glu Ser Leu Asp Thr Gln Ile Lys Val
Asn Tyr Gly Gln Tyr Tyr Ser 355 360 365 Glu Gly Asp Ser Asn Leu Lys
Ser Met Ile Thr Ser Glu Asp Pro Lys 370 375 380 Thr Gly Glu Tyr Asp
Gln Tyr Ile Tyr Val Asn Pro Leu Gln Lys Thr 385 390 395 400 Ala Asn
Gly Thr Val Val Arg Val Gln Gly Phe Gln Val Asp Pro Thr 405 410 415
Lys Ser Asn Gly Gln Val Lys Pro Asp Thr Thr Gln Ile Lys Ile Leu 420
425 430 Lys Val Ala Asp Gly Gln Pro Leu Asn Ser Ser Phe Gly Val Asn
Asp 435 440 445 Ser Glu Tyr Glu Asp Val Thr Lys Gln Phe Asn Ile Val
Tyr Arg Asp 450 455 460 Asn Asn Leu Ala Asp Ile Tyr Phe Gly Asn Leu
Asn Gly Gln Arg Tyr 465 470 475 480 Ile Val Lys Val Thr Ser Lys Glu
Asn Leu Asp Ser Lys Glu Asp Leu 485 490 495 Asn Leu Arg Ala Ile Met
Ala Thr Gln Asn Arg Tyr Gly Gln Tyr Asn 500 505 510 Tyr Ile Thr Trp
Asp Asn Asp Ile Val Lys Ser Ser Ser Gly Gly Thr 515 520 525 Ala Asp
Gly Asn Glu Ala Ser Tyr Gln Leu Gly Asp Lys Val Trp Asn 530 535 540
Asp Val Asn Lys Asn Gly Ile Gln Asp Gln Gly Glu Thr Gly Ile Ala 545
550 555 560 Asp Val Lys Val Thr Leu Lys Asp Leu Asp Gly Asn Ile Leu
Asp Thr 565 570 575 Thr Tyr Thr Asn Thr Asn Gly Lys Tyr Ile Phe Asp
Asn Leu Lys Asn 580 585 590 Gly Asn Tyr Gln Val Gly Phe Glu Thr Pro
Glu Gly Tyr Ala Ala Ser 595 600 605 Pro Ser Asn Gln Gly Asn Asp Ala
Leu Asp Ser Asp Gly Pro Thr Asn 610 615 620 Val Gln Ala Val Ile Ser
Asp Gly Asn Asn Leu Thr Ile Asp Gln Gly 625 630 635 640 Phe Tyr Gln
Thr Glu Thr Pro Thr His Asn Val Gly Asp Lys Val Trp 645 650 655 Glu
Asp Leu Asn Lys Asp Gly Ile Gln Asp Gln Asn Glu Pro Gly Ile 660 665
670 Ala Asn Val Lys Val Thr Leu Lys Asp Ala Asp Gly Asn Val Val Asp
675 680 685 Thr Arg Thr Thr Asp Asp Lys Gly Asn Tyr Leu Phe Glu Lys
Val Lys 690 695 700 Glu Gly Glu Tyr Thr Ile Glu Phe Glu Thr Pro Glu
Gly Tyr Thr Pro 705 710 715 720 Thr Gln Thr Gly Gln Gly Arg Val Ser
Thr Asp Ser Asn Gly Thr Ser 725 730 735 Ser Leu Ile Leu Val Glu Gly
Asn Asp Asp Leu Thr Ile Asp Ser Gly 740 745 750 Phe Tyr Lys Glu Pro
Val Thr His Lys Val Gly Asp Lys Val Trp Asp 755 760 765 Asp Leu Asn
Lys Asp Gly Ile Gln Asp Asp Asn Glu Pro Gly Ile Ser 770 775 780 Asp
Val Lys Val Thr Leu Lys Asp Ala Asp Gly Asn Val Val Asp Thr 785 790
795 800 Arg Thr Thr Asp Ala Asn Gly Asn Tyr Leu Phe Glu Asn Val Lys
Glu 805 810 815 Gly Asp Tyr Thr Ile Glu Phe Glu Thr Pro Glu Gly Tyr
Thr Pro Thr 820 825 830 Val Thr Gly Gln Gly Thr Ala Asp Asn Asp Ser
Asn Gly Thr Ser Thr 835 840 845 Lys Val Thr Val Lys Asp Gly Asp Asp
Leu Thr Ile Asp Ser Gly Phe 850 855 860 Thr Gln Val Thr Pro Glu Pro
Pro Thr His Asn Val Gly Asp Lys Val 865 870 875 880 Trp Asp Asp Leu
Asn Lys Asp Gly Ile Gln Asp Asp Asn Glu Pro Gly 885 890 895 Ile Ser
Asp Val Lys Val Thr Leu Lys Asp Ala Asp Gly Asn Val Val 900 905 910
Asp Thr Arg Thr Thr Asp Ala Asn Gly Asn Tyr Leu Phe Glu Asn Val 915
920 925 Lys Glu Gly Asp Tyr Thr Ile Glu Phe Glu Thr Pro Glu Gly Tyr
Thr 930 935 940 Pro Thr Val Thr Gly Gln Gly Thr Ala Asp Asn Asp Ser
Asn Gly Thr 945 950 955 960 Ser Thr Lys Val Thr Val Lys Asp Gly Asp
Asp Leu Thr Ile Asp Ser 965 970 975 Gly Phe Thr
Gln Val Thr Pro Glu Pro Pro Thr Glu Pro Glu Asn Pro 980 985 990 Ser
Pro Glu Gln Pro Ser Glu Pro Gly Gln Pro Glu Asn Pro Ser Pro 995
1000 1005 Glu Gln Pro Ser Glu Pro Gly Gln Pro Glu Asn Pro Ser Pro
Glu 1010 1015 1020 Gln Pro Ser Glu Pro Gly Gln Pro Glu Asn Pro Ser
Pro Glu Gln 1025 1030 1035 Pro Ser Glu Pro Gly Gln Pro Glu Asn Pro
Ser Pro Glu Gln Pro 1040 1045 1050 Ser Glu Pro Gly Gln Pro Lys Asn
Pro Ser Pro Glu Gln Pro Asn 1055 1060 1065 Asn Pro Ser Val Pro Gly
Val Gln Asn Pro Glu Lys Pro Ser Leu 1070 1075 1080 Thr Pro Val Thr
Gln Pro Val His Ser Asn Gly Asn Lys Ala Lys 1085 1090 1095 Pro Ser
Gln Gln Gln Lys Ala Leu Pro Glu Thr Gly Glu Thr Glu 1100 1105 1110
Ser His Gln Gly Thr Leu Phe Gly Gly Ile Leu Ala Ala Leu Gly 1115
1120 1125 Ala Leu Leu Phe Ala Arg Lys Lys Arg His Asp Lys Lys Gln
Ser 1130 1135 1140 His 29660DNAStaphylococcus pseudintermedius
29atgaagaaaa caatttcagt acttggtcta gggctattag caacattttt tgtaagtaac
60gaatcatatg ccgcagaaac gattcaaaac aatacgtcat caagtgaaac gaatcaaaat
120tcagatcaga cgccgttaga tcattatatt cgaaaagcag atggcacact
ggttgaaccg 180aacgtgtacc cacataaaga ttatgtagag aatgaaggac
ctttaccaga gtttaaattt 240caagttgact ctaagaaaga ttcatctgat
ccaaatcaag caccgttaga tcattatatt 300cgaaaagcgg atggcacgtt
ggttgaaccg aatgtatatc cacacaaaga ttatgtcgaa 360aatgaagggc
ctttaccaga gtttaaattt atgtatgctg acaaacaaaa tcatcatgac
420caacagagta aaaacaacaa ggataagcag cgtgcaaatt acagtgacaa
aaagcataat 480gatcagccgg gtcatccaaa agcagtcacg ccagctgtac
aacatgataa agcagtcact 540tcaaacgcta ctgtaaaagc attgccaaac
acaggtgaat ctgataaaac aacacaatta 600ccaatcgtat tatcattgtt
atctgtgggg attttagttt tattaaaatt gagaaaataa
66030219PRTStaphylococcus pseudintermedius 30Met Lys Lys Thr Ile
Ser Val Leu Gly Leu Gly Leu Leu Ala Thr Phe 1 5 10 15 Phe Val Ser
Asn Glu Ser Tyr Ala Ala Glu Thr Ile Gln Asn Asn Thr 20 25 30 Ser
Ser Ser Glu Thr Asn Gln Asn Ser Asp Gln Thr Pro Leu Asp His 35 40
45 Tyr Ile Arg Lys Ala Asp Gly Thr Leu Val Glu Pro Asn Val Tyr Pro
50 55 60 His Lys Asp Tyr Val Glu Asn Glu Gly Pro Leu Pro Glu Phe
Lys Phe 65 70 75 80 Gln Val Asp Ser Lys Lys Asp Ser Ser Asp Pro Asn
Gln Ala Pro Leu 85 90 95 Asp His Tyr Ile Arg Lys Ala Asp Gly Thr
Leu Val Glu Pro Asn Val 100 105 110 Tyr Pro His Lys Asp Tyr Val Glu
Asn Glu Gly Pro Leu Pro Glu Phe 115 120 125 Lys Phe Met Tyr Ala Asp
Lys Gln Asn His His Asp Gln Gln Ser Lys 130 135 140 Asn Asn Lys Asp
Lys Gln Arg Ala Asn Tyr Ser Asp Lys Lys His Asn 145 150 155 160 Asp
Gln Pro Gly His Pro Lys Ala Val Thr Pro Ala Val Gln His Asp 165 170
175 Lys Ala Val Thr Ser Asn Ala Thr Val Lys Ala Leu Pro Asn Thr Gly
180 185 190 Glu Ser Asp Lys Thr Thr Gln Leu Pro Ile Val Leu Ser Leu
Leu Ser 195 200 205 Val Gly Ile Leu Val Leu Leu Lys Leu Arg Lys 210
215 315541DNAStaphylococcus pseudintermedius 31atgaaaagta
aatatgattt tttacctaat agacttaata aattttctat acgaaaattt 60actgttggta
gtgtatcagt gctaatagga gccactttat tattcgggtt tgtagaagga
120gaagcatcag catcagtaaa agaaggtcaa caaagtataa attctagtga
gaaagaaagc 180gccgatccta cagtagttga tttaattagt aagaaagaaa
caaatttaga tggactagat 240gtatcaagag aagaaacgac caaagtacca
ataaatgaaa acaaaagagg tgaggaacaa 300agtatttctg ataaagctat
aacagaaaaa gctgatacac cagtaagcaa tttatcaagt 360aaggaagttg
aggagcaagg tgtttctgat aaagctataa cagaaaaagc tgatacacca
420gtaaccaatt tatcaagtaa ggaagctaag gagcaaggtg cttctgatag
agttataaca 480gaaaaagctg atacaccagt aagcaattta tcaagtaagg
aagctaagga gcaaggtgct 540tctgatagag ttataacaga aaaagctgat
acaccagtaa gcaatttatc aagtaaggaa 600gttgaggagc aaggtgtttc
tgataaagct atagagaaaa tagctgatgc atcagctact 660gatttgtcaa
gtaaggaaga agtagaacaa gatatatcta cacaaggtaa agtaaaatca
720aaggaagcag tacaagtaga aagtagtcag ttacaaaatt taaatagtga
aataaatgct 780gaacctaatg aaattaaggc aatagataga agttcaatat
tacctttaaa tttaaatgat 840gaagaaaata acaaaaaagt taataaaggg
actcgggttc cagaagctac attaagaaat 900gcctctaata accaactcaa
tacacgaatg agatcagtga gtttatttag agttgctaga 960ctaacagaaa
tcaatagaaa tgttaatgat aaagtaaagg tttcggatat cgacatcgca
1020atagccccac cgcatactaa ccctaaaact ggaaaagaag aattttgggc
gacatcttct 1080tcagttttaa agttaaaggc aagctatgaa ttggataata
gcatttctaa aggggatcaa 1140tttactattc aatttggtca aaatattcgt
ccaggtggat taaatttacc aagaccttat 1200aattttttat atgataagga
taaaaaatta gttgcaactg gccgttacaa taaagaatca 1260aatacaatca
catatacatt tacggattat gtagataaac atcaaaacat taaaggtagt
1320tttgagatga atgcattttc tagaaaggaa aatgctacta ctgacaaaac
agcatatcca 1380atggatgtta ctattgcgaa tcaaaaatat agtgaaaata
ttattgtaga ctatggtaat 1440aaaaagaatg ctgctattat ttcaagtaca
gaatatattg atttagatgg tagtagaaaa 1500atgacaacat atattaatca
aaatggtagt aaaaattcca tctatcgtgc tgatatgcaa 1560attgatttga
acggttataa atttgatcca tccaaaaaca attttaaaat ttatgaagtg
1620gaaaatagca gtgactttgt ggatagcttt tcaccagatg tgagcaagtt
aagggatgtt 1680acgagtcaat ttaatattca atatacaaat aataatacaa
tggcaaaagt ggattttggt 1740actaaccttt ggaggggtaa aaaatatatt
attcagcaag tggcgaatat agacgacagt 1800aaattagtga aaaatgcttc
aatcaattat acattgaata aaatggattt taataataaa 1860agaacggtag
aaacacataa caatacttat tctacagtga aagataaatc aacagcacta
1920ggtgacgtac aggaaagtca atctattagt gagagccaat cagttagtga
aagcgagtca 1980ctaagtgaga gccaatcaat cagtgaaagc gaatcattaa
gtgagagcca atcaatcagt 2040gaaagcgaat cattaagtga aagtcaatca
atctcagaga gcgaatcact aagtgaaagt 2100cagtcaattt cagaaagcga
atcattaagt gaaagccaat caatctcaga gagtgaatca 2160ttaagtgaaa
gtcagtcaat ttcagagagt gaatcactaa gtgaaagtca gtcaatttca
2220gaaagcgaat cattaagcga gagtcagtca atttcagaaa gcgaatcatt
aagcgagagt 2280cagtcaattt cagaaagcga atcattaagt gaaagccaat
caatcagtga aagcgaatca 2340ctaagcgaga gccaatcaat ctcagagagt
gaatcattaa gcgagagtca atcaatctca 2400gagagcgaat cattaagtga
gagtcaatca atcagtgaaa gcgagtcact aagtgagagt 2460caatcaattt
cagagagcga atcattaagt gaaagccaat caatctcaga gagtgaatca
2520ctaagtgaga gccaatcaat ctcagagagt gaatcattaa gtgagagcca
atcaatctca 2580gagagcgagt cactaagcga gagccaatca atttcagaga
gtgaatcact aagtgaaagt 2640caatcaattt cagagagcga atcactaagt
gagagccaat caatctcaga gagcgaatca 2700ctaagtgaaa gtcaatcaat
ttcagagagt gaatcactaa gcgagagcca atcaatctca 2760gagagtgaat
cattaagtga aagtcagtca atttcagaga gtgaatcact aagtgaaagt
2820cagtcaattt cagaaagcga atcattaagt gaaagccaat caatcagtga
aagcgaatca 2880ctaagcgaga gtcaatcaat ctcagagagc gaatcattaa
gtgaaagtca atcaatttca 2940gaaagcgagt cattaagcga gagtcagtca
atctcagaga gcgaatcact aagcgagagt 3000caatcaatct cagagagtga
atcattaagt gagagccaat cagttagtga aagcgaatca 3060ctaagtgaaa
gtcagtcaat ttcagaaagc gaatcattaa gtgagagtca atcaatttca
3120gaaagcgaat cattaagtga aagccaatca atcagtgaaa gcgaatcact
aagcgagagc 3180caatcaatca gtgaaagcga atcattaagt gagagtcaat
caatctcaga aagcgaatca 3240ttaagtgaga gtcaatcaat cagtgaaagc
gaatcactaa gcgagagcca atcaatctca 3300gagagcgaat cactaagcga
gagccaatca atctcagaga gcgagtcact aagcgagagc 3360caatcaatca
gtgaaagcga atcattaagt gagagtcaat caatcagtga aagcgagtca
3420ctaagtgaga gccaatcaat ctcagagagt gaatcattga gtgagagcca
atcaatctca 3480gagagcgagt cactaagtga gagtcaatca atttcagaga
gcgaatcatt aagtgaaagc 3540caatcaatct cagagagtga atcattgagt
gagagccaat cagttagtga aagcgagtca 3600ctaagtgaga gtcaatcaat
cagtgaaagc gagtcactaa gtgagagtca atcaatttca 3660gagagcgaat
cattaagcga gagtcagtca atctcagaga gtgaatcact aagtgagagc
3720caatcaatct cagagagtga atcattaagt gagagccaat caatctcaga
gagtgaatca 3780ctaagtgaga gtcaatcaat cagtgaaagc gaatcactaa
gcgagagcca atcaatttca 3840gagagtgaat cattaagtga gagccaatca
gttagtgaaa gcgaatcact aagcgagagc 3900caatcaatct cagagagcga
atcattgagt gagagccaat caatctcaga gagtgaatca 3960ttgagtgaga
gtcaatcaat cagtgaaagc gaatcactaa gcgaaagtca atcaatttca
4020gagagtgaat cattgagtga gagccaatca atttcagaga gtgaatcact
aagtgaaagt 4080cagtcaattt cagaaagcga atcactaagc gagagccaat
caatctcaga gagcgaatca 4140ctaagtgaaa gtcagtcaat ttcagaaagc
gaatcattaa gtgaaagcca atcaatctca 4200gagagtgaat cattaagtga
aagtcagtca atttcagaga gtgaatcact aagtgaaagt 4260cagtcaattt
cagaaagcga atcattaagc gagagtcagt caatttcaga aagcgaatca
4320ttaagtgaaa gccaatcaat cagtgaaagc gaatcactaa gcgagagcca
atcaatctca 4380gagagcgaat cactaagcga gagccaatca atctcagaga
gcgaatcact aagtgaaagt 4440caatcaattt cagagagtga atcattgagt
gagagtcaat caatttcaga gagtgaatca 4500ctaagtgaaa gtcaatcaat
ttcagagagt gaatcactaa gcgagagcca atcaatctca 4560gagagtgaat
cattaagtga aagtcagtca atttcagaga gggaatcact aagtgaaagt
4620cagtcaattt cagaaagcga atcattaagt gaaagccaat caatcagtga
aagcgaatca 4680ctaagtgaaa gtcaatcaat ctcagagagt gaatcactaa
gtgagagcca atcaatctca 4740gagagtgaat cattgagtga gagccaatca
atctcagaga gcgaatcact aagtgaaagt 4800caatcaattt cagaaagcga
gtcattaagc gagagtcagt caatctcaga gagtgaatca 4860ctaagtgaga
gccaatcaat ctcagagagt gaatcactaa gtgagagtca atcaatcagt
4920gaaagcgaat cactaagcga gagccaatca atttcagaga gtgaatcatt
aagtgagagc 4980caatcagtta gtgaaagcga atcactaagc gagagccaat
caatctcaga gagcgagtca 5040ctaagcgaga gtcaatcaat ctcagagagt
gaatcactaa gtgaaagtca gtcaatttca 5100gaaagcgagt cactaagcga
gagtcaatca atctcagaga gtgaatcatt gagtgagagc 5160caatcaatct
cagagagcga atcattgagt gagagccaat caatctcaga gagtgaatca
5220ttgagtgaga gccaatcaat ttcagagagc gaatcactaa gcgagagcca
atcaatcagt 5280gaaagcgaat cattaagtga gagtcagtca attagcgaaa
gcgaatcact aagtgagagt 5340caatcaatct cagagagtga atcactaagt
gaaagtcagt caatcagcga aagcgaatct 5400aaatctttac ctaataccgg
tactggagaa aagatttcta attatccagg tattttagga 5460ggattattaa
gcatattagg tataagtttg cttaaaagaa aagacagaga gaaaaaatta
5520ggacaaaaat ctaataagta g 5541321846PRTStaphylococcus
pseudintermedius 32Met Lys Ser Lys Tyr Asp Phe Leu Pro Asn Arg Leu
Asn Lys Phe Ser 1 5 10 15 Ile Arg Lys Phe Thr Val Gly Ser Val Ser
Val Leu Ile Gly Ala Thr 20 25 30 Leu Leu Phe Gly Phe Val Glu Gly
Glu Ala Ser Ala Ser Val Lys Glu 35 40 45 Gly Gln Gln Ser Ile Asn
Ser Ser Glu Lys Glu Ser Ala Asp Pro Thr 50 55 60 Val Val Asp Leu
Ile Ser Lys Lys Glu Thr Asn Leu Asp Gly Leu Asp 65 70 75 80 Val Ser
Arg Glu Glu Thr Thr Lys Val Pro Ile Asn Glu Asn Lys Arg 85 90 95
Gly Glu Glu Gln Ser Ile Ser Asp Lys Ala Ile Thr Glu Lys Ala Asp 100
105 110 Thr Pro Val Ser Asn Leu Ser Ser Lys Glu Val Glu Glu Gln Gly
Val 115 120 125 Ser Asp Lys Ala Ile Thr Glu Lys Ala Asp Thr Pro Val
Thr Asn Leu 130 135 140 Ser Ser Lys Glu Ala Lys Glu Gln Gly Ala Ser
Asp Arg Val Ile Thr 145 150 155 160 Glu Lys Ala Asp Thr Pro Val Ser
Asn Leu Ser Ser Lys Glu Ala Lys 165 170 175 Glu Gln Gly Ala Ser Asp
Arg Val Ile Thr Glu Lys Ala Asp Thr Pro 180 185 190 Val Ser Asn Leu
Ser Ser Lys Glu Val Glu Glu Gln Gly Val Ser Asp 195 200 205 Lys Ala
Ile Glu Lys Ile Ala Asp Ala Ser Ala Thr Asp Leu Ser Ser 210 215 220
Lys Glu Glu Val Glu Gln Asp Ile Ser Thr Gln Gly Lys Val Lys Ser 225
230 235 240 Lys Glu Ala Val Gln Val Glu Ser Ser Gln Leu Gln Asn Leu
Asn Ser 245 250 255 Glu Ile Asn Ala Glu Pro Asn Glu Ile Lys Ala Ile
Asp Arg Ser Ser 260 265 270 Ile Leu Pro Leu Asn Leu Asn Asp Glu Glu
Asn Asn Lys Lys Val Asn 275 280 285 Lys Gly Thr Arg Val Pro Glu Ala
Thr Leu Arg Asn Ala Ser Asn Asn 290 295 300 Gln Leu Asn Thr Arg Met
Arg Ser Val Ser Leu Phe Arg Val Ala Arg 305 310 315 320 Leu Thr Glu
Ile Asn Arg Asn Val Asn Asp Lys Val Lys Val Ser Asp 325 330 335 Ile
Asp Ile Ala Ile Ala Pro Pro His Thr Asn Pro Lys Thr Gly Lys 340 345
350 Glu Glu Phe Trp Ala Thr Ser Ser Ser Val Leu Lys Leu Lys Ala Ser
355 360 365 Tyr Glu Leu Asp Asn Ser Ile Ser Lys Gly Asp Gln Phe Thr
Ile Gln 370 375 380 Phe Gly Gln Asn Ile Arg Pro Gly Gly Leu Asn Leu
Pro Arg Pro Tyr 385 390 395 400 Asn Phe Leu Tyr Asp Lys Asp Lys Lys
Leu Val Ala Thr Gly Arg Tyr 405 410 415 Asn Lys Glu Ser Asn Thr Ile
Thr Tyr Thr Phe Thr Asp Tyr Val Asp 420 425 430 Lys His Gln Asn Ile
Lys Gly Ser Phe Glu Met Asn Ala Phe Ser Arg 435 440 445 Lys Glu Asn
Ala Thr Thr Asp Lys Thr Ala Tyr Pro Met Asp Val Thr 450 455 460 Ile
Ala Asn Gln Lys Tyr Ser Glu Asn Ile Ile Val Asp Tyr Gly Asn 465 470
475 480 Lys Lys Asn Ala Ala Ile Ile Ser Ser Thr Glu Tyr Ile Asp Leu
Asp 485 490 495 Gly Ser Arg Lys Met Thr Thr Tyr Ile Asn Gln Asn Gly
Ser Lys Asn 500 505 510 Ser Ile Tyr Arg Ala Asp Met Gln Ile Asp Leu
Asn Gly Tyr Lys Phe 515 520 525 Asp Pro Ser Lys Asn Asn Phe Lys Ile
Tyr Glu Val Glu Asn Ser Ser 530 535 540 Asp Phe Val Asp Ser Phe Ser
Pro Asp Val Ser Lys Leu Arg Asp Val 545 550 555 560 Thr Ser Gln Phe
Asn Ile Gln Tyr Thr Asn Asn Asn Thr Met Ala Lys 565 570 575 Val Asp
Phe Gly Thr Asn Leu Trp Arg Gly Lys Lys Tyr Ile Ile Gln 580 585 590
Gln Val Ala Asn Ile Asp Asp Ser Lys Leu Val Lys Asn Ala Ser Ile 595
600 605 Asn Tyr Thr Leu Asn Lys Met Asp Phe Asn Asn Lys Arg Thr Val
Glu 610 615 620 Thr His Asn Asn Thr Tyr Ser Thr Val Lys Asp Lys Ser
Thr Ala Leu 625 630 635 640 Gly Asp Val Gln Glu Ser Gln Ser Ile Ser
Glu Ser Gln Ser Val Ser 645 650 655 Glu Ser Glu Ser Leu Ser Glu Ser
Gln Ser Ile Ser Glu Ser Glu Ser 660 665 670 Leu Ser Glu Ser Gln Ser
Ile Ser Glu Ser Glu Ser Leu Ser Glu Ser 675 680 685 Gln Ser Ile Ser
Glu Ser Glu Ser Leu Ser Glu Ser Gln Ser Ile Ser 690 695 700 Glu Ser
Glu Ser Leu Ser Glu Ser Gln Ser Ile Ser Glu Ser Glu Ser 705 710 715
720 Leu Ser Glu Ser Gln Ser Ile Ser Glu Ser Glu Ser Leu Ser Glu Ser
725 730 735 Gln Ser Ile Ser Glu Ser Glu Ser Leu Ser Glu Ser Gln Ser
Ile Ser 740 745 750 Glu Ser Glu Ser Leu Ser Glu Ser Gln Ser Ile Ser
Glu Ser Glu Ser 755 760 765 Leu Ser Glu Ser Gln Ser Ile Ser Glu Ser
Glu Ser Leu Ser Glu Ser 770 775 780 Gln Ser Ile Ser Glu Ser Glu Ser
Leu Ser Glu Ser Gln Ser Ile Ser 785 790 795 800 Glu Ser Glu Ser Leu
Ser Glu Ser Gln Ser Ile Ser Glu Ser Glu Ser 805 810 815 Leu Ser Glu
Ser Gln Ser Ile Ser Glu Ser Glu Ser Leu Ser Glu Ser 820 825 830 Gln
Ser Ile Ser Glu Ser Glu Ser Leu Ser Glu Ser Gln Ser Ile Ser 835 840
845 Glu Ser Glu Ser Leu Ser Glu Ser Gln Ser Ile Ser Glu Ser Glu Ser
850 855 860 Leu Ser Glu Ser Gln Ser Ile Ser Glu Ser Glu Ser Leu Ser
Glu Ser 865 870 875 880 Gln Ser Ile Ser Glu Ser Glu Ser Leu Ser Glu
Ser Gln Ser Ile Ser 885 890 895 Glu Ser Glu Ser Leu Ser Glu Ser Gln
Ser Ile Ser Glu Ser Glu Ser 900 905 910 Leu Ser Glu Ser Gln Ser Ile
Ser Glu Ser Glu Ser Leu Ser Glu Ser 915 920 925 Gln Ser Ile Ser Glu
Ser Glu
Ser Leu Ser Glu Ser Gln Ser Ile Ser 930 935 940 Glu Ser Glu Ser Leu
Ser Glu Ser Gln Ser Ile Ser Glu Ser Glu Ser 945 950 955 960 Leu Ser
Glu Ser Gln Ser Ile Ser Glu Ser Glu Ser Leu Ser Glu Ser 965 970 975
Gln Ser Ile Ser Glu Ser Glu Ser Leu Ser Glu Ser Gln Ser Ile Ser 980
985 990 Glu Ser Glu Ser Leu Ser Glu Ser Gln Ser Ile Ser Glu Ser Glu
Ser 995 1000 1005 Leu Ser Glu Ser Gln Ser Val Ser Glu Ser Glu Ser
Leu Ser Glu 1010 1015 1020 Ser Gln Ser Ile Ser Glu Ser Glu Ser Leu
Ser Glu Ser Gln Ser 1025 1030 1035 Ile Ser Glu Ser Glu Ser Leu Ser
Glu Ser Gln Ser Ile Ser Glu 1040 1045 1050 Ser Glu Ser Leu Ser Glu
Ser Gln Ser Ile Ser Glu Ser Glu Ser 1055 1060 1065 Leu Ser Glu Ser
Gln Ser Ile Ser Glu Ser Glu Ser Leu Ser Glu 1070 1075 1080 Ser Gln
Ser Ile Ser Glu Ser Glu Ser Leu Ser Glu Ser Gln Ser 1085 1090 1095
Ile Ser Glu Ser Glu Ser Leu Ser Glu Ser Gln Ser Ile Ser Glu 1100
1105 1110 Ser Glu Ser Leu Ser Glu Ser Gln Ser Ile Ser Glu Ser Glu
Ser 1115 1120 1125 Leu Ser Glu Ser Gln Ser Ile Ser Glu Ser Glu Ser
Leu Ser Glu 1130 1135 1140 Ser Gln Ser Ile Ser Glu Ser Glu Ser Leu
Ser Glu Ser Gln Ser 1145 1150 1155 Ile Ser Glu Ser Glu Ser Leu Ser
Glu Ser Gln Ser Ile Ser Glu 1160 1165 1170 Ser Glu Ser Leu Ser Glu
Ser Gln Ser Ile Ser Glu Ser Glu Ser 1175 1180 1185 Leu Ser Glu Ser
Gln Ser Val Ser Glu Ser Glu Ser Leu Ser Glu 1190 1195 1200 Ser Gln
Ser Ile Ser Glu Ser Glu Ser Leu Ser Glu Ser Gln Ser 1205 1210 1215
Ile Ser Glu Ser Glu Ser Leu Ser Glu Ser Gln Ser Ile Ser Glu 1220
1225 1230 Ser Glu Ser Leu Ser Glu Ser Gln Ser Ile Ser Glu Ser Glu
Ser 1235 1240 1245 Leu Ser Glu Ser Gln Ser Ile Ser Glu Ser Glu Ser
Leu Ser Glu 1250 1255 1260 Ser Gln Ser Ile Ser Glu Ser Glu Ser Leu
Ser Glu Ser Gln Ser 1265 1270 1275 Ile Ser Glu Ser Glu Ser Leu Ser
Glu Ser Gln Ser Val Ser Glu 1280 1285 1290 Ser Glu Ser Leu Ser Glu
Ser Gln Ser Ile Ser Glu Ser Glu Ser 1295 1300 1305 Leu Ser Glu Ser
Gln Ser Ile Ser Glu Ser Glu Ser Leu Ser Glu 1310 1315 1320 Ser Gln
Ser Ile Ser Glu Ser Glu Ser Leu Ser Glu Ser Gln Ser 1325 1330 1335
Ile Ser Glu Ser Glu Ser Leu Ser Glu Ser Gln Ser Ile Ser Glu 1340
1345 1350 Ser Glu Ser Leu Ser Glu Ser Gln Ser Ile Ser Glu Ser Glu
Ser 1355 1360 1365 Leu Ser Glu Ser Gln Ser Ile Ser Glu Ser Glu Ser
Leu Ser Glu 1370 1375 1380 Ser Gln Ser Ile Ser Glu Ser Glu Ser Leu
Ser Glu Ser Gln Ser 1385 1390 1395 Ile Ser Glu Ser Glu Ser Leu Ser
Glu Ser Gln Ser Ile Ser Glu 1400 1405 1410 Ser Glu Ser Leu Ser Glu
Ser Gln Ser Ile Ser Glu Ser Glu Ser 1415 1420 1425 Leu Ser Glu Ser
Gln Ser Ile Ser Glu Ser Glu Ser Leu Ser Glu 1430 1435 1440 Ser Gln
Ser Ile Ser Glu Ser Glu Ser Leu Ser Glu Ser Gln Ser 1445 1450 1455
Ile Ser Glu Ser Glu Ser Leu Ser Glu Ser Gln Ser Ile Ser Glu 1460
1465 1470 Ser Glu Ser Leu Ser Glu Ser Gln Ser Ile Ser Glu Ser Glu
Ser 1475 1480 1485 Leu Ser Glu Ser Gln Ser Ile Ser Glu Ser Glu Ser
Leu Ser Glu 1490 1495 1500 Ser Gln Ser Ile Ser Glu Ser Glu Ser Leu
Ser Glu Ser Gln Ser 1505 1510 1515 Ile Ser Glu Ser Glu Ser Leu Ser
Glu Ser Gln Ser Ile Ser Glu 1520 1525 1530 Arg Glu Ser Leu Ser Glu
Ser Gln Ser Ile Ser Glu Ser Glu Ser 1535 1540 1545 Leu Ser Glu Ser
Gln Ser Ile Ser Glu Ser Glu Ser Leu Ser Glu 1550 1555 1560 Ser Gln
Ser Ile Ser Glu Ser Glu Ser Leu Ser Glu Ser Gln Ser 1565 1570 1575
Ile Ser Glu Ser Glu Ser Leu Ser Glu Ser Gln Ser Ile Ser Glu 1580
1585 1590 Ser Glu Ser Leu Ser Glu Ser Gln Ser Ile Ser Glu Ser Glu
Ser 1595 1600 1605 Leu Ser Glu Ser Gln Ser Ile Ser Glu Ser Glu Ser
Leu Ser Glu 1610 1615 1620 Ser Gln Ser Ile Ser Glu Ser Glu Ser Leu
Ser Glu Ser Gln Ser 1625 1630 1635 Ile Ser Glu Ser Glu Ser Leu Ser
Glu Ser Gln Ser Ile Ser Glu 1640 1645 1650 Ser Glu Ser Leu Ser Glu
Ser Gln Ser Val Ser Glu Ser Glu Ser 1655 1660 1665 Leu Ser Glu Ser
Gln Ser Ile Ser Glu Ser Glu Ser Leu Ser Glu 1670 1675 1680 Ser Gln
Ser Ile Ser Glu Ser Glu Ser Leu Ser Glu Ser Gln Ser 1685 1690 1695
Ile Ser Glu Ser Glu Ser Leu Ser Glu Ser Gln Ser Ile Ser Glu 1700
1705 1710 Ser Glu Ser Leu Ser Glu Ser Gln Ser Ile Ser Glu Ser Glu
Ser 1715 1720 1725 Leu Ser Glu Ser Gln Ser Ile Ser Glu Ser Glu Ser
Leu Ser Glu 1730 1735 1740 Ser Gln Ser Ile Ser Glu Ser Glu Ser Leu
Ser Glu Ser Gln Ser 1745 1750 1755 Ile Ser Glu Ser Glu Ser Leu Ser
Glu Ser Gln Ser Ile Ser Glu 1760 1765 1770 Ser Glu Ser Leu Ser Glu
Ser Gln Ser Ile Ser Glu Ser Glu Ser 1775 1780 1785 Leu Ser Glu Ser
Gln Ser Ile Ser Glu Ser Glu Ser Lys Ser Leu 1790 1795 1800 Pro Asn
Thr Gly Thr Gly Glu Lys Ile Ser Asn Tyr Pro Gly Ile 1805 1810 1815
Leu Gly Gly Leu Leu Ser Ile Leu Gly Ile Ser Leu Leu Lys Arg 1820
1825 1830 Lys Asp Arg Glu Lys Lys Leu Gly Gln Lys Ser Asn Lys 1835
1840 1845 331953DNAStaphylococcus pseudintermedius 33atgttaagaa
caaattataa actaagaaag cttaaagtag gtttagtatc gacaggtgtg 60gcgttgactt
ttgtgatggc aagtgggaat gcagaggcgt cggagaacga gcagactgaa
120gtaaaagggg aggcgcaagt tgcttctgtg aatgaaaaag agagtgaagc
agaattacct 180gtagcgcaac aagaagcatc tattcaacta gacaaagtac
aaccaggcga tgcacagctt 240tcaggctata cacagccaaa caaagcgatt
tctgtaaaga tcgacaataa agatattgtg 300tctgtagatg atggctatga
agaggtatta tcggatgata caggtaaatt tgtatatgat 360ttgaaagggc
gtcaaattgt ttacaatcaa aaagttgatg ttgaagcgat gacgccattt
420aattttgaag attttgatga atcagcactt gagagcgaag aggcattgga
ggcgttaggt 480caattggaag acgaagaaac agcgacagct tctgtgacga
cgcctagata tgaaggtgcg 540tatacagttc ctgaagaacg cttgacaccc
attcaaggcc aacagcaagt attcatcgaa 600cctattttag aaggggcaag
taaaatcaaa ggacatacat ctgtacaagg taaagtcgcg 660ttagcaatca
atcaagaaca tgtgcaccta ggtgatacgt tagaagaaca agcagcactc
720actgatcaag agtggcaagg tcgttatgac gggatttggc gccatattga
tgatcaaggg 780tttttcgagt ttgacttgaa ccgtctttac aataaatctt
acccattgaa gtctggcgat 840ttagtgactt tatcttttaa atctaatgac
gaagtaggcc cattattcaa tgtgaacgtt 900gagcctttcg aacgtgtggc
acaagctaaa acaaagtatg agcagaatga cagtccagta 960gtcaacaaat
tggatgatac taaaagtgac ttggaggttc aacctatcta tggagacctt
1020acacaagcag cagtacatgg cgagtcgaaa gtgttgatac cggggacgtc
aaaagttgaa 1080ggacgtacga attatgcaca tgcatggata gagatggcat
ctaatttagg ggaatatcgt 1140agtttcccta aattacaagc tgatgcgaca
ggtgcgttta tatttgattt aaaagcggca 1200gacatacaat tgttaaacgg
agaacgtttg acattcagag ccgttgaccc acatacaaaa 1260caacagttag
ctgaaactac atcagaagta cgcccagtag atatgcaaga tgaagagtca
1320gaggttgtgc agacttcaag cactgagaaa tcagcacttg cggatgaaat
tcttcgttct 1380atgacaattg acaaatcatt taatcctgaa gttaccgaga
taccgggtca tgtatatcct 1440aagaaaacag aggataaagg tgctgaaaat
acagaacaag cctcagagaa ttctgagaag 1500ccatctcaga ctacagaatc
tcaaaatgat gccgtacaag atgtagagaa atcctctgtt 1560aatgaggagg
ttacgccacc ttcaacagaa tctgctcaag ttgaaaaggg gcaaaataca
1620gaaggggctt tgcttccaaa aaatgtagaa caacatgtag agagtatacc
ataccaaaaa 1680cgtaaagcgt tgataggact gacaaaacat caaggatcag
ggcacatgcc gccattttct 1740ttaagcttta ataataaaga agatgacgta
tccacaaagg ttaacgaagc aaacgagcat 1800gaacgtaagc agggtacagt
ttatccagag caaatagaac aattacctca aacaggttta 1860actgaaaaat
cgccattctg ggcattgtta tttgttgtat caggcacagg tttattatta
1920ttcaaacgtt ctagacgaca acgccaatct taa 195334650PRTStaphylococcus
pseudintermedius 34Met Leu Arg Thr Asn Tyr Lys Leu Arg Lys Leu Lys
Val Gly Leu Val 1 5 10 15 Ser Thr Gly Val Ala Leu Thr Phe Val Met
Ala Ser Gly Asn Ala Glu 20 25 30 Ala Ser Glu Asn Glu Gln Thr Glu
Val Lys Gly Glu Ala Gln Val Ala 35 40 45 Ser Val Asn Glu Lys Glu
Ser Glu Ala Glu Leu Pro Val Ala Gln Gln 50 55 60 Glu Ala Ser Ile
Gln Leu Asp Lys Val Gln Pro Gly Asp Ala Gln Leu 65 70 75 80 Ser Gly
Tyr Thr Gln Pro Asn Lys Ala Ile Ser Val Lys Ile Asp Asn 85 90 95
Lys Asp Ile Val Ser Val Asp Asp Gly Tyr Glu Glu Val Leu Ser Asp 100
105 110 Asp Thr Gly Lys Phe Val Tyr Asp Leu Lys Gly Arg Gln Ile Val
Tyr 115 120 125 Asn Gln Lys Val Asp Val Glu Ala Met Thr Pro Phe Asn
Phe Glu Asp 130 135 140 Phe Asp Glu Ser Ala Leu Glu Ser Glu Glu Ala
Leu Glu Ala Leu Gly 145 150 155 160 Gln Leu Glu Asp Glu Glu Thr Ala
Thr Ala Ser Val Thr Thr Pro Arg 165 170 175 Tyr Glu Gly Ala Tyr Thr
Val Pro Glu Glu Arg Leu Thr Pro Ile Gln 180 185 190 Gly Gln Gln Gln
Val Phe Ile Glu Pro Ile Leu Glu Gly Ala Ser Lys 195 200 205 Ile Lys
Gly His Thr Ser Val Gln Gly Lys Val Ala Leu Ala Ile Asn 210 215 220
Gln Glu His Val His Leu Gly Asp Thr Leu Glu Glu Gln Ala Ala Leu 225
230 235 240 Thr Asp Gln Glu Trp Gln Gly Arg Tyr Asp Gly Ile Trp Arg
His Ile 245 250 255 Asp Asp Gln Gly Phe Phe Glu Phe Asp Leu Asn Arg
Leu Tyr Asn Lys 260 265 270 Ser Tyr Pro Leu Lys Ser Gly Asp Leu Val
Thr Leu Ser Phe Lys Ser 275 280 285 Asn Asp Glu Val Gly Pro Leu Phe
Asn Val Asn Val Glu Pro Phe Glu 290 295 300 Arg Val Ala Gln Ala Lys
Thr Lys Tyr Glu Gln Asn Asp Ser Pro Val 305 310 315 320 Val Asn Lys
Leu Asp Asp Thr Lys Ser Asp Leu Glu Val Gln Pro Ile 325 330 335 Tyr
Gly Asp Leu Thr Gln Ala Ala Val His Gly Glu Ser Lys Val Leu 340 345
350 Ile Pro Gly Thr Ser Lys Val Glu Gly Arg Thr Asn Tyr Ala His Ala
355 360 365 Trp Ile Glu Met Ala Ser Asn Leu Gly Glu Tyr Arg Ser Phe
Pro Lys 370 375 380 Leu Gln Ala Asp Ala Thr Gly Ala Phe Ile Phe Asp
Leu Lys Ala Ala 385 390 395 400 Asp Ile Gln Leu Leu Asn Gly Glu Arg
Leu Thr Phe Arg Ala Val Asp 405 410 415 Pro His Thr Lys Gln Gln Leu
Ala Glu Thr Thr Ser Glu Val Arg Pro 420 425 430 Val Asp Met Gln Asp
Glu Glu Ser Glu Val Val Gln Thr Ser Ser Thr 435 440 445 Glu Lys Ser
Ala Leu Ala Asp Glu Ile Leu Arg Ser Met Thr Ile Asp 450 455 460 Lys
Ser Phe Asn Pro Glu Val Thr Glu Ile Pro Gly His Val Tyr Pro 465 470
475 480 Lys Lys Thr Glu Asp Lys Gly Ala Glu Asn Thr Glu Gln Ala Ser
Glu 485 490 495 Asn Ser Glu Lys Pro Ser Gln Thr Thr Glu Ser Gln Asn
Asp Ala Val 500 505 510 Gln Asp Val Glu Lys Ser Ser Val Asn Glu Glu
Val Thr Pro Pro Ser 515 520 525 Thr Glu Ser Ala Gln Val Glu Lys Gly
Gln Asn Thr Glu Gly Ala Leu 530 535 540 Leu Pro Lys Asn Val Glu Gln
His Val Glu Ser Ile Pro Tyr Gln Lys 545 550 555 560 Arg Lys Ala Leu
Ile Gly Leu Thr Lys His Gln Gly Ser Gly His Met 565 570 575 Pro Pro
Phe Ser Leu Ser Phe Asn Asn Lys Glu Asp Asp Val Ser Thr 580 585 590
Lys Val Asn Glu Ala Asn Glu His Glu Arg Lys Gln Gly Thr Val Tyr 595
600 605 Pro Glu Gln Ile Glu Gln Leu Pro Gln Thr Gly Leu Thr Glu Lys
Ser 610 615 620 Pro Phe Trp Ala Leu Leu Phe Val Val Ser Gly Thr Gly
Leu Leu Leu 625 630 635 640 Phe Lys Arg Ser Arg Arg Gln Arg Gln Ser
645 650 351491DNAStaphylococcus pseudintermedius 35atgaaaacta
aatacacagc aaaattatta attggggcag caacaatatc tttagcaaca 60tttatttcac
aagggaacgc acatgcgagc gaacaaacta caggactcgc accggcacaa
120cctgtcaact ttgattcaat caatgtaacg ccagaccaaa aaacattcta
tcaagtctta 180catatggaag gcatttcaga agaccaacgt gaacaatatt
tgaaacaatt gcacgaagac 240ccaagtagcg cacaaaatgt tttttcagaa
tcaattaaag atgccatcca cccggaacgt 300cgtgttgcgc aacaaaatgc
gttttacagc gtattacaca acgatgactt atccgaagag 360caacgtgatg
catacattgg tagaattaaa gaagatccag atcaaagcca agaagtattt
420gttgagtctt taaatgtggc acctaaagca gaatcacatg aagatcgcct
cattgaatta 480caaaacaaaa atttaatgga agcgaatgaa gcacttaaag
cgttacaaca agaagacagc 540attcagaata gacgtgcggc tcaacgtgct
gtcaacaaat tgacgccgga tagcgcgaac 600gcattccaaa aagaattaga
tcaaatcaat gccccacgcg acgctaaaat taaagctgac 660gctgaagcaa
aaaaacaagc acctgaagta agcgcaccac aaattgaaga tgcacctact
720actgaagttg caccatctcc aaaacaagat atgccaaaag tagataaaaa
agaagaagat 780aaagtagaaa gtgatactga ggtcaaagaa gtacctaaag
ctgatacaga gaaaaaccct 840caatctaaag acacttctaa aactgaacaa
gctaaagaaa cacctaaagt agagcaatca 900cctaaaacag aaaaggctga
agaagcacct aaagcagaaa cacctcaaaa tggaaataaa 960gcacaaactg
aagaagctaa accagaagta aaagacaatg tgaaaaacac tccatctgca
1020cctgtgttac ctgaaacagg aaaagcaaca acttcaacac ttgaaagcta
ctggaattct 1080ttcaaagaca gtgtgaataa aggttatact tacattaaac
aaagcttaga aagtggttat 1140caatatttaa aaggtcaata cgactatatc
actaaaaaat acaatgatgc gaaatactat 1200acaaaaatgt attcaaatca
taagtctaca attgatcagt ctgtattagc tatattaggt 1260aaaactggat
ctagcgcata tatcaagcca ttaaatatcg aagaaaattc aaacgtattt
1320tacaaagctt atgcaaaaac aagaaacttt gctacagaaa gcattaacac
aggaaaagta 1380ttatacacat tatatcaaaa ccctactgta gttaaatctg
ctttcactgc aattgaaaca 1440gcaaatacag taaaaaatgc aataagcaat
cttttctctc tcttcaaata a 149136496PRTStaphylococcus pseudintermedius
36Met Lys Thr Lys Tyr Thr Ala Lys Leu Leu Ile Gly Ala Ala Thr Ile 1
5 10 15 Ser Leu Ala Thr Phe Ile Ser Gln Gly Asn Ala His Ala Ser Glu
Gln 20 25 30 Thr Thr Gly Leu Ala Pro Ala Gln Pro Val Asn Phe Asp
Ser Ile Asn 35 40 45 Val Thr Pro Asp Gln Lys Thr Phe Tyr Gln Val
Leu His Met Glu Gly 50 55 60 Ile Ser Glu Asp Gln Arg Glu Gln Tyr
Leu Lys Gln Leu His Glu Asp 65 70 75 80 Pro Ser Ser Ala Gln Asn Val
Phe Ser Glu Ser Ile Lys Asp Ala Ile 85 90 95 His Pro Glu Arg Arg
Val Ala Gln Gln Asn Ala Phe Tyr Ser Val Leu 100 105 110 His Asn Asp
Asp Leu Ser Glu Glu Gln Arg Asp Ala Tyr Ile Gly Arg 115 120 125 Ile
Lys Glu Asp Pro Asp Gln Ser Gln Glu Val Phe Val Glu Ser Leu 130
135
140 Asn Val Ala Pro Lys Ala Glu Ser His Glu Asp Arg Leu Ile Glu Leu
145 150 155 160 Gln Asn Lys Asn Leu Met Glu Ala Asn Glu Ala Leu Lys
Ala Leu Gln 165 170 175 Gln Glu Asp Ser Ile Gln Asn Arg Arg Ala Ala
Gln Arg Ala Val Asn 180 185 190 Lys Leu Thr Pro Asp Ser Ala Asn Ala
Phe Gln Lys Glu Leu Asp Gln 195 200 205 Ile Asn Ala Pro Arg Asp Ala
Lys Ile Lys Ala Asp Ala Glu Ala Lys 210 215 220 Lys Gln Ala Pro Glu
Val Ser Ala Pro Gln Ile Glu Asp Ala Pro Thr 225 230 235 240 Thr Glu
Val Ala Pro Ser Pro Lys Gln Asp Met Pro Lys Val Asp Lys 245 250 255
Lys Glu Glu Asp Lys Val Glu Ser Asp Thr Glu Val Lys Glu Val Pro 260
265 270 Lys Ala Asp Thr Glu Lys Asn Pro Gln Ser Lys Asp Thr Ser Lys
Thr 275 280 285 Glu Gln Ala Lys Glu Thr Pro Lys Val Glu Gln Ser Pro
Lys Thr Glu 290 295 300 Lys Ala Glu Glu Ala Pro Lys Ala Glu Thr Pro
Gln Asn Gly Asn Lys 305 310 315 320 Ala Gln Thr Glu Glu Ala Lys Pro
Glu Val Lys Asp Asn Val Lys Asn 325 330 335 Thr Pro Ser Ala Pro Val
Leu Pro Glu Thr Gly Lys Ala Thr Thr Ser 340 345 350 Thr Leu Glu Ser
Tyr Trp Asn Ser Phe Lys Asp Ser Val Asn Lys Gly 355 360 365 Tyr Thr
Tyr Ile Lys Gln Ser Leu Glu Ser Gly Tyr Gln Tyr Leu Lys 370 375 380
Gly Gln Tyr Asp Tyr Ile Thr Lys Lys Tyr Asn Asp Ala Lys Tyr Tyr 385
390 395 400 Thr Lys Met Tyr Ser Asn His Lys Ser Thr Ile Asp Gln Ser
Val Leu 405 410 415 Ala Ile Leu Gly Lys Thr Gly Ser Ser Ala Tyr Ile
Lys Pro Leu Asn 420 425 430 Ile Glu Glu Asn Ser Asn Val Phe Tyr Lys
Ala Tyr Ala Lys Thr Arg 435 440 445 Asn Phe Ala Thr Glu Ser Ile Asn
Thr Gly Lys Val Leu Tyr Thr Leu 450 455 460 Tyr Gln Asn Pro Thr Val
Val Lys Ser Ala Phe Thr Ala Ile Glu Thr 465 470 475 480 Ala Asn Thr
Val Lys Asn Ala Ile Ser Asn Leu Phe Ser Leu Phe Lys 485 490 495
37504PRTStaphylococcus pseudintermedius 37Asn Glu Asp Val Thr Glu
Thr Thr Gly Arg Asn Ser Val Thr Thr Gln 1 5 10 15 Ala Ser Glu Gln
His Leu Gln Val Glu Ala Val Pro Gln Glu Gly Asn 20 25 30 Asn Val
Asn Val Ser Ser Val Lys Val Pro Thr Asn Thr Ala Thr Gln 35 40 45
Ala Gln Glu Asp Val Ala Ser Val Ser Asp Val Lys Ala His Ala Asp 50
55 60 Asp Ala Leu Gln Val Gln Glu Ser Ser His Thr Asp Gly Val Ser
Ser 65 70 75 80 Glu Phe Lys Gln Glu Thr Ala Tyr Ala Asn Pro Gln Thr
Ala Glu Thr 85 90 95 Val Lys Pro Asn Ser Glu Ala Val His Gln Ser
Glu Tyr Glu Asp Lys 100 105 110 Gln Lys Pro Val Ser Ser Ser Arg Lys
Glu Asp Glu Thr Met Leu Gln 115 120 125 Gln Gln Gln Val Glu Ala Lys
Asn Val Val Ser Ala Glu Glu Val Ser 130 135 140 Lys Glu Glu Asn Thr
Gln Val Met Gln Ser Pro Gln Asp Val Glu Gln 145 150 155 160 His Val
Gly Gly Lys Asp Ile Ser Asn Glu Val Val Val Asp Arg Ser 165 170 175
Asp Ile Lys Gly Phe Asn Ser Glu Thr Thr Ile Arg Pro His Gln Gly 180
185 190 Gln Gly Gly Arg Leu Asn Tyr Gln Leu Lys Phe Pro Ser Asn Val
Lys 195 200 205 Pro Gly Asp Gln Phe Thr Ile Lys Leu Ser Asp Asn Ile
Asn Thr His 210 215 220 Gly Val Ser Val Glu Arg Thr Ala Pro Arg Ile
Met Ala Lys Asn Thr 225 230 235 240 Glu Gly Ala Thr Asp Val Ile Ala
Glu Gly Leu Val Leu Glu Asp Gly 245 250 255 Lys Thr Ile Val Tyr Thr
Phe Lys Asp Tyr Val Asn Gly Lys Gln Asn 260 265 270 Leu Thr Ala Glu
Leu Ser Val Ser Tyr Phe Val Ser Pro Glu Lys Val 275 280 285 Leu Thr
Thr Gly Thr Gln Thr Phe Thr Thr Met Ile Gly Asn His Ser 290 295 300
Thr Gln Ser Asn Ile Asp Val Tyr Tyr Asp Asn Ser His Tyr Val Asp 305
310 315 320 Gly Arg Ile Ser Gln Val Asn Lys Lys Glu Ala Lys Phe Gln
Gln Ile 325 330 335 Ala Tyr Ile Asn Pro Asn Gly Tyr Leu Asn Gly Arg
Gly Thr Ile Ala 340 345 350 Val Asn Gly Glu Val Val Ser Gly Thr Thr
Lys Asp Leu Met Gln Pro 355 360 365 Thr Val Arg Val Tyr Gln Tyr Lys
Gly Gln Gly Val Pro Pro Glu Ser 370 375 380 Ile Thr Ile Asp Pro Asn
Met Trp Glu Glu Ile Ser Ile Asn Asp Thr 385 390 395 400 Met Val Arg
Lys Tyr Asp Gly Gly Tyr Ser Leu Asn Leu Asp Thr Ser 405 410 415 Lys
Asn Gln Lys Tyr Ala Ile Tyr Tyr Glu Gly Ala Tyr Asp Ala Gln 420 425
430 Ala Asp Thr Leu Leu Tyr Arg Thr Tyr Ile Gln Ser Leu Asn Ser Tyr
435 440 445 Tyr Pro Phe Ser Tyr Gln Lys Met Asn Gly Val Lys Phe Tyr
Glu Asn 450 455 460 Ser Ala Ser Gly Ser Gly Glu Leu Lys Pro Lys Pro
Pro Glu Gln Pro 465 470 475 480 Lys Pro Glu Pro Glu Ile Gln Ala Asp
Val Val Asp Ile Ile Glu Asp 485 490 495 Ser His Val Ile Asp Ile Gly
Trp 500 381512DNAStaphylococcus pseudintermedius 38aatgaagatg
tcactgaaac aactgggaga aattcagtga caacgcaagc ttctgagcaa 60catttgcaag
tggaagcagt acctcaagaa ggcaataatg taaatgtatc ctctgtaaaa
120gtacctacga atacggcaac gcaagcacaa gaagatgttg caagtgtatc
cgatgttaaa 180gcacatgctg atgatgcatt acaagtacaa gaaagtagtc
atactgatgg tgtttcttca 240gaattcaagc aggagacagc ttatgcgaat
cctcaaacag ctgagacagt taaacctaat 300agtgaagcag tgcatcagtc
tgaatacgag gataagcaaa aacccgtatc atctagccgc 360aaagaagatg
agactatgct tcagcagcaa caagttgaag ccaaaaatgt tgtgagtgcg
420gaggaagtgt ctaaagaaga aaatactcaa gtgatgcaat cccctcaaga
cgttgaacaa 480catgtaggtg gtaaagatat ctctaatgag gttgtagtgg
ataggagtga tatcaaagga 540tttaacagcg aaactactat tcgacctcat
cagggacaag gtggtaggtt gaattatcaa 600ttaaagtttc ctagcaatgt
aaagccaggc gatcagttta ctataaaatt atctgacaat 660atcaatacac
atggtgtttc tgttgaaaga accgcaccga gaatcatggc taaaaatact
720gaaggtgcga cggatgtaat tgctgaaggt ctagtgttgg aagatggtaa
aaccatcgta 780tatacattta aagactatgt aaatggcaag caaaatttga
ctgctgagtt atcagtgagc 840tatttcgtaa gtccggaaaa agtcttgact
actgggacac aaacattcac gacgatgatc 900ggtaatcatt caacgcaatc
caatattgac gtttattatg ataatagtca ttatgtagat 960ggacgtattt
cgcaagtgaa caaaaaagaa gctaaatttc aacaaatagc atacattaac
1020cctaatggct atttaaatgg cagggggaca attgcagtta atggtgaagt
ggtcagtggt 1080acgactaaag acttaatgca acctacagtg cgtgtatatc
aatataaagg acaaggtgtt 1140cctcctgaaa gtattactat agaccctaat
atgtgggaag aaatcagcat aaacgatact 1200atggtaagaa aatatgatgg
tggctatagc ttgaatctgg ataccagcaa gaatcaaaaa 1260tatgccatct
attatgaagg ggcatatgat gcgcaagctg acacactgtt gtatagaaca
1320tatatacagt cattaaacag ttactatccg ttcagttacc aaaaaatgaa
cggtgtgaag 1380ttttacgaaa acagtgcgag tggaagcggt gagttgaaac
cgaaaccacc tgaacaacca 1440aaaccagaac ctgaaattca agctgatgta
gtagatatta ttgaagatag ccatgtgatt 1500gatataggat gg 1512
* * * * *
References