U.S. patent application number 15/892661 was filed with the patent office on 2018-08-02 for methods and compositions for inhibiting cd32b expressing cells.
This patent application is currently assigned to XENCOR, INC.. The applicant listed for this patent is XENCOR, INC.. Invention is credited to Seung Yup Chu, John R. Desjarlais, Sher Bahadur Karki, Gregory Alan Lazar, Gregory L. Moore, Igor Vostiar.
Application Number | 20180215821 15/892661 |
Document ID | / |
Family ID | 39772872 |
Filed Date | 2018-08-02 |
United States Patent
Application |
20180215821 |
Kind Code |
A1 |
Chu; Seung Yup ; et
al. |
August 2, 2018 |
METHODS AND COMPOSITIONS FOR INHIBITING CD32B EXPRESSING CELLS
Abstract
The present invention relates to immunoglobulins that bind
Fc.gamma.RIIb+ cells and coengage the antigen on the cell's surface
and an Fc.gamma.RIIb on the cell's surface, methods for their
generation, and methods for using the immunoglobulins.
Inventors: |
Chu; Seung Yup; (Cypress,
CA) ; Desjarlais; John R.; (Pasadena, CA) ;
Karki; Sher Bahadur; (Pomona, CA) ; Lazar; Gregory
Alan; (Monrovia, CA) ; Moore; Gregory L.;
(Monrovia, CA) ; Vostiar; Igor; (Monrovia,
CA) |
|
Applicant: |
Name |
City |
State |
Country |
Type |
XENCOR, INC. |
Monrovia |
CA |
US |
|
|
Assignee: |
XENCOR, INC.
Monrovia
CA
|
Family ID: |
39772872 |
Appl. No.: |
15/892661 |
Filed: |
February 9, 2018 |
Related U.S. Patent Documents
|
|
|
|
|
|
Application
Number |
Filing Date |
Patent Number |
|
|
14328593 |
Jul 10, 2014 |
9914778 |
|
|
15892661 |
|
|
|
|
13594619 |
Aug 24, 2012 |
9079960 |
|
|
14328593 |
|
|
|
|
13301464 |
Nov 21, 2011 |
|
|
|
13594619 |
|
|
|
|
12156183 |
May 30, 2008 |
8063187 |
|
|
13301464 |
|
|
|
|
60940776 |
May 30, 2007 |
|
|
|
60953174 |
Jul 31, 2007 |
|
|
|
60970413 |
Sep 6, 2007 |
|
|
|
60976279 |
Sep 28, 2007 |
|
|
|
60990509 |
Nov 27, 2007 |
|
|
|
61012035 |
Dec 6, 2007 |
|
|
|
61013775 |
Dec 14, 2007 |
|
|
|
61019395 |
Jan 7, 2008 |
|
|
|
61032059 |
Feb 27, 2008 |
|
|
|
61043585 |
Apr 9, 2008 |
|
|
|
61046397 |
Apr 18, 2008 |
|
|
|
Current U.S.
Class: |
1/1 |
Current CPC
Class: |
A61P 19/02 20180101;
C07K 2317/24 20130101; C07K 2317/524 20130101; C07K 2317/734
20130101; C07K 16/00 20130101; C07K 2317/53 20130101; C07K 16/2851
20130101; C07K 2317/73 20130101; C07K 2317/77 20130101; A61P 35/00
20180101; A61P 29/00 20180101; C07K 2317/71 20130101; A61P 1/00
20180101; C07K 2317/732 20130101; C07K 16/2878 20130101; A61K
2039/505 20130101; C07K 2317/92 20130101; A61P 37/06 20180101; C07K
2317/31 20130101; C07K 16/2803 20130101; C07K 2317/72 20130101;
A61P 3/10 20180101; A61P 37/08 20180101; C07K 2317/76 20130101;
C07K 16/283 20130101; C07K 2317/52 20130101 |
International
Class: |
C07K 16/28 20060101
C07K016/28; C07K 16/00 20060101 C07K016/00 |
Claims
1. (canceled)
2. (canceled)
3. A nucleic acid encoding an Fc variant comprising at least amino
acid modifications G236N and S267E as compared to a parent Fc
polypeptide, wherein numbering is according to the EU index
according to Kabat.
4. An expression vector comprising the nucleic acid of claim 3.
5. A host cell comprising the vector of claim 4.
6. A method of treating an autoimmune disease comprising
administering a protein comprising an Fc variant comprising at
least amino acid modifications G236N and S267E as compared to a
parent Fc polypeptide, wherein numbering is according to the EU
index according to Kabat.
7. (canceled)
8. A nucleic acid encoding a heavy chain of an antibody, the heavy
chain comprising an Fc variant comprising at least amino acid
modifications G236N and S267E as compared to a parent Fc
polypeptide, wherein numbering is according to the EU index
according to Kabat.
9. An expression vector comprising the nucleic acid of claim 8.
10. A host cell comprising the vector of claim 9.
Description
RELATED APPLICATIONS
[0001] This application claims benefit under 35 U.S.C .sctn. 120 to
U.S. application Ser. No. 13/594,619 (filed Aug. 24, 2012); which
claims benefit under 35 U.S.C .sctn. 120 to U.S. application Ser.
No. 13/301,464 (filed Nov. 21, 2011); which claims benefit under 35
U.S.C .sctn. 120 to U.S. application Ser. No. 12/156,183 (filed May
30, 2008), now U.S. Pat. No. 8,063,187; which claims benefit under
35 U.S.C .sctn. 119 (e) to U.S. Provisional Application Nos.
60/940,776 (filed May 30, 2007); 60/953,174 (filed Jul. 31, 2007);
60/970,413 (filed Sep. 6, 2007); 60/976,279 (filed Sep. 28, 2007);
60/990,509 (filed Nov. 27, 2007); 61/012,035 (filed Dec. 6, 2007);
61/013,775 (filed Dec. 14, 2007), 61/019,395 (filed Jan. 7, 2008),
61/032,059 (filed Feb. 27, 2008), 61/043,585 (filed Apr. 9, 2008),
and 61/046,397 (filed Apr. 18, 2008). Each of the above
applications is incorporated in its entirety by reference.
SEQUENCE LISTING
[0002] A Sequence Listing submitted in computer readable form (CRF)
is hereby incorporated by reference.
TECHNICAL FIELD
[0003] The present disclosure relates to methods of inhibiting
cells that express the Fc gamma receptor CD32b (Fc.gamma.RIIb),
immunoglobulin compositions that may be useful for such methods,
and application of such compositions for treating immune disorders
and hematological malignancies.
BACKGROUND
[0004] Antigen recognition by B cells is mediated by the B cell
receptor (BCR), a surface-bound immunoglobulin in complex with
signaling components CD79a (Ig.alpha.) and CD79b (Ig.beta.).
Crosslinking of BCR upon engagement of antigen results in
phosphorylation of immunoreceptor tyrosine-based activation motifs
(ITAMs) within CD79a and CD79b, initiating a cascade of
intracellular signaling events that recruit downstream molecules to
the membrane and stimulate calcium mobilization. This leads to the
induction of diverse B cell responses (e.g., cell survival,
proliferation, antibody production, antigen presentation,
differentiation, etc.) which lead to a humoral immune response
(DeFranco, A. L., 1997, Curr. Opin. Immunol. 9, 296-308; Pierce, S.
K., 2002, Nat. Rev. Immunol. 2, 96-105; Ravetch, J. V. &
Lanier, L. L., 2000, Science 290, 84-89). Other components of the
BCR coreceptor complex enhance (e.g., CD19, CD21, and CD81) or
suppress (e.g., CD22 and CD72) BCR activation signals (Doody, G. M.
et al., 1996, Curr. Opin. Immunol. 8, 378-382; Li, D. H. et al.,
2006, J. Immunol. 176, 5321-5328). In this way, the immune system
maintains multiple BCR regulatory mechanisms to ensure that B cell
responses are tightly controlled.
[0005] When antibodies are produced to an antigen, the circulating
level of immune complexes (e.g., antigen bound to antibody)
increases. These immune complexes downregulate antigen-induced B
cell activation. It is believed that these immune complexes
downregulate antigen-induced B cell activation by coengaging
cognate BCR with the low-affinity inhibitory receptor
Fc.gamma.RIIb, the only IgG receptor on B cells (Heyman, B., 2003,
Immunol. Lett. 88, 157-161). It is also believed that this negative
feedback of antibody production requires interaction of the
antibody Fc domain with Fc.gamma.RIIb since immune complexes
containing F(ab').sub.2 antibody fragments are not inhibitory
(Chan, P. L. & Sinclair, N. R., 1973, Immunology 24, 289-301).
The intracellular immunoreceptor tyrosine-based inhibitory motif
(ITIM) of Fc.gamma.RIIb is necessary to inhibit BCR-induced
intracellular signals (Amigorena, S. et al., 1992, Science 256,
1808-1812; Muta, T., et al., 1994, Nature 368, 70-73). This
inhibitory effect occurs through phosphorylation of the
Fc.gamma.RIIb ITIM, which recruits SH2-containing inositol
polyphosphate 5-phosphatase (SHIP) to neutralize ITAM-induced
intracellular calcium mobilization (Kiener, P. A., et al., 1997, J.
Biol. Chem. 272, 3838-3844; Ono, M., et al., 1996, Nature 383,
263-266; Ravetch, J. V. & Lanier, L. L., 2000, Science 290,
84-89). In addition, Fc.gamma.RIIb-mediated SHIP phosphorylation
inhibits the downstream Ras-MAPK proliferation pathway
(Tridandapani, S. et al., 1998, Immunol. 35, 1135-1146).
SUMMARY OF EXEMPLARY EMBODIMENTS
[0006] The present disclosure provides novel immunoglobulins,
compositions comprising such immunoglobulins, and methods of using
the immunoglobulin to inhibit cells that express Fc.gamma.RIIb. The
Fc.gamma.RIIb.sup.+ cell inhibitory methods disclosed herein
comprise contacting Fc.gamma.RIIb.sup.+ cells with an
immunoglobulin that binds Fc.gamma.RIIb and coengages a target
antigen on the cell's surface and an Fc.gamma.RIIb on the cell's
surface. In one embodiment, the immunoglobulin binds with
Fc.gamma.RIIb, wherein the affinity of said binding has a Kd less
than about 100 nM, e.g., less than or equal to about 95 nM, less
than or equal to about 90 nM, less than or equal to about 85 nM,
less than or equal to about 80 nM, less than or equal to about 75
nM, less than or equal to about 74 nM. In one embodiment, the
immunoglobulin comprises an Fc region, wherein said Fc region
comprises one or more modifications compared to a parent Fc region,
wherein said modifications are at positions selected from the group
consisting of 234, 235, 236, 237, 239, 265, 266, 267, 268, 298,
325, 326, 327, 328, 329, 330, 331, and 332, wherein numbering is
according to the EU index. In another embodiment, the
immunoglobulin is a bispecific antibody comprising a first Fv
region and a second Fv region, wherein said first Fv region binds
the target antigen, and said second Fv region binds Fc.gamma.RIIb
with a Kd of less than about 100 nM. In another embodiment, the
immunoglobulin is an Fc fusion comprising an Fc region, wherein
said Fc region binds Fc.gamma.RIIb with a Kd of less than about 100
nM. Fc.gamma.RIIb.sup.+ cells as disclosed herein may be cancer
cells, B cells, plasma cells, dendritic cells, macrophages,
neutrophils, mast cells, basophils, eosinophils, and a combination
thereof.
[0007] Also disclosed herein are novel methods of inhibiting
activation of B cells. The B cell inhibitory methods disclosed
herein comprise contacting B cells with an immunoglobulin that
binds Fc.gamma.RIIb and coengages a target antigen on the B cell's
surface and an Fc.gamma.RIIb on the B cell's surface. In one
embodiment, the immunoglobulin binds with Fc.gamma.RIIb, wherein
the affinity of said binding has a Kd less than about 100 nM, e.g.,
less than or equal to about 95 nM, less than or equal to about 90
nM, less than or equal to about 85 nM, less than or equal to about
80 nM, less than or equal to about 75 nM, less than or equal to
about 74 nM. In one embodiment, the immunoglobulin comprises an Fc
region, wherein said Fc region comprises one or more modifications
compared to a parent Fc region, wherein said modifications are at
positions selected from the group consisting of 234, 235, 236, 237,
239, 265, 266, 267, 268, 298, 325, 326, 327, 328, 329, 330, 331,
and 332, wherein numbering is according to the EU index. In another
embodiment, the immunoglobulin is a bispecific antibody comprising
a first Fv region and a second Fv region, wherein said first Fv
region binds the target antigen, and said second Fv region binds
Fc.gamma.RIIb with a Kd of less than about 100 nM. In another
embodiment, the immunoglobulin is an Fc fusion comprising an Fc
region, wherein said Fc region binds Fc.gamma.RIIb with a Kd of
less than about 100 nM. In one embodiment, the immunoglobulin binds
at least two B cell proteins, .e.g., at least two proteins bound,
or that may be bound, on the surface of B cells. In one embodiment,
the first of said B cell proteins is Fc.gamma.RIIb and the second
of said B cell proteins is part of the B cell receptor (BCR)
complex. In another embodiment, the second of said B cell proteins
is not involved directly in antigen recognition. In another
embodiment, the second of said B cell proteins is an antigen bound
to the BCR complex. In some embodiments, the immunoglobulins
inhibit release of calcium from the B cells upon their stimulation
through the B cell receptor. In another embodiment, an
immunoglobulin disclosed herein binds at least two B cell proteins
bound on the surface of the same B cell.
[0008] Also disclosed herein are novel methods of treating B
cell-mediated disorders, e.g., autoimmune diseases, inflammatory
diseases, hematological malignancies, etc. The treatment methods
disclosed herein comprise administration to a patient in need of
such administration a therapeutic amount of an immunoglobulin that
binds Fc.gamma.RIIb.sup.+ cells and coengages a target antigen on
the cell's surface and an Fc.gamma.RIIb on cell's surface. In one
embodiment, the immunoglobulin binds with Fc.gamma.RIIb, wherein
the affinity of said binding has a Kd less than about 100 nM, e.g.,
less than or equal to about 95 nM, less than or equal to about 90
nM, less than or equal to about 85 nM, less than or equal to about
80 nM, less than or equal to about 75 nM, less than or equal to
about 74 nM. In one embodiment, the immunoglobulin comprises an Fc
region, wherein said Fc region comprises one or more modifications
compared to a parent Fc region, wherein said modifications are at
positions selected from the group consisting of 234, 235, 236, 237,
239, 265, 266, 267, 268, 298, 325, 326, 327, 328, 329, 330, 331,
and 332, wherein numbering is according to the EU index. In another
embodiment, the immunoglobulin is a bispecific antibody comprising
a first Fv region and a second Fv region, wherein said first Fv
region binds the target antigen, and said second Fv region binds
Fc.gamma.RIIb with a Kd of less than about 100 nM. In another
embodiment, the immunoglobulin is an Fc fusion comprising an Fc
region, wherein said Fc region binds Fc.gamma.RIIb with a Kd of
less than about 100 nM. In some embodiments, autoimmune and
inflammatory diseases that may be treated by the methods disclosed
herein include Systemic Lupus Erythematosus, Rheumatoid arthritis,
Sjogren's syndrome, Multiple sclerosis, Idiopathic thrombocytopenic
purpura (ITP), Graves disease, Inflammatory bowel disease,
Psoriasis, Type I diabetes, and Asthma.
[0009] Disclosed herein are novel Fc.gamma.RIIb+ cell inhibitory
immunoglobulin compositions. The compositions disclosed herein
include immunoglobulins that bind Fc.gamma.RIIb+ cells and coengage
a target antigen on the cell's surface and an Fc.gamma.RIIb on
cell's surface. In one embodiment, the immunoglobulin binds with
Fc.gamma.RIIb, wherein the affinity of said binding has a Kd less
than about 100 nM, e.g., less than or equal to about 95 nM, less
than or equal to about 90 nM, less than or equal to about 85 nM,
less than or equal to about 80 nM, less than or equal to about 75
nM, less than or equal to about 74 nM. In one embodiment, the
immunoglobulin comprises an Fc region, wherein said Fc region
comprises one or more modifications compared to a parent Fc region,
wherein said modifications are at positions selected from the group
consisting of 234, 235, 236, 237, 239, 265, 266, 267, 268, 298,
325, 326, 327, 328, 329, 330, 331, and 332, wherein numbering is
according to the EU index. In another embodiment, the
immunoglobulin is a bispecific antibody comprising a first Fv
region and a second Fv region, wherein said first Fv region binds
the target antigen, and said second Fv region binds Fc.gamma.RIIb
with a Kd of less than about 100 nM. In another embodiment, the
immunoglobulin is an Fc fusion comprising an Fc region, wherein
said Fc region binds Fc.gamma.RIIb with a Kd of less than about 100
nM.
[0010] In some embodiments, the immunoglobulins that bind
Fc.gamma.RIIb+ cells and coengage a target antigen on the cell's
surface and an Fc.gamma.RIIb on cell's surface disclosed herein may
bind and/or coengage a target antigen selected from the group
consisting of: CD19, CD20, CD21 (CR2), CD22, CD23/Fc.epsilon.RII,
Fc.epsilon.RI, (.alpha., .beta., and .gamma. subunits),
CD24/BBA-1/HSA, CD27, CD35 (CR1), CD38, CD40, CD45RA,
CD52/CAMPATH-1/HE5, CD72, CD79a (Ig.alpha.), CD79b (Ig.beta., IgM
(.mu.), CD80, CD81, CD86, Leu13, HLA-DR, -DP, -DQ, CD138,
CD317/HM1.24, CD11a, CD11b, CD11c, CD14, CD68, CD163, CD172a,
CD200R, and CD206. In other embodiments, the immunoglobulins that
bind Fc.gamma.RIIb+ cells and coengage a target antigen on the
cell's surface and an Fc.gamma.RIIb on cell's surface disclosed
herein may bind and/or coengage a target antigen selected from the
group consisting of: IgM (.mu.), CD19, CD20, CD21, CD22, CD23,
CD24, CD35, CD40, CD45RA, CD72, CD79a, CD79b, CD80, CD81, CD86, and
HLA-DR. In one embodiment, immunoglobulins that bind Fc.gamma.RIIb+
cells and coengage a target antigen on the cell's surface and an
Fc.gamma.RIIb on cell's surface disclosed herein may bind and/or
coengage a target antigen selected from the group consisting of:
IgM (.mu.), CD79a, CD79b, CD19, CD21, CD22, CD72, CD81, and Leu13.
In one embodiment, immunoglobulins that bind Fc.gamma.RIIb+ cells
and coengage a target antigen on the cell's surface and an
Fc.gamma.RIIb on cell's surface disclosed herein may bind and/or
coengage a target antigen selected from the group consisting of:
IgM (.mu.), CD19, CD79a, CD79b, CD81, and HLA-DR. In another
embodiment, immunoglobulins that bind Fc.gamma.RIIb+ cells and
coengage a target antigen on the cell's surface and an
Fc.gamma.RIIb on cell's surface disclosed herein may bind and/or
coengage a target antigen selected from the group consisting of:
CD22, CD40, and CD72.
[0011] In one embodiment, the immunoglobulins that bind
Fc.gamma.RIIb+ cells and coengage a target antigen on the cell's
surface and an Fc.gamma.RIIb on cell's surface disclosed herein may
bind and/or coengage an autoantigen or allergen. In an alternate
embodiment, an immunoglobulin disclosed herein may be an Fc fusion
that is covalently linked to an autoantigen or allergen. In one
embodiment, the autoantigen is selected from the group consisting
citrullinated proteins and peptides such as CCP-1, CCP-2 (cyclical
citrullinated peptides), fibrinogen, fibrin, vimentin, fillaggrin,
collagen I and II peptides, alpha-enolase, translation initiation
factor 4G1, perinuclear factor, keratin, Sa (cytoskeletal protein
vimentin), components of articular cartilage such as collagen II,
IX, and XI, circulating serum proteins such as RFs (IgG, IgM),
fibrinogen, plasminogen, ferritin, nuclear components such as
RA33/hnRNP A2, Sm, eukaryotic trasnlation elogation factor 1 alpha
1, stress proteins such as HSP-65, -70, -90, BiP,
inflammatory/immune factors such as B7-H1, IL-1 alpha, and IL-8,
enzymes such as calpastatin, alpha-enolase, aldolase-A, dipeptidyl
peptidase, osteopontin, glucose-6-phosphate isomerase, receptors
such as lipocortin 1, neutrophil nuclear proteins such as
lactoferrin and 25-35 kD nuclear protein, granular proteins such as
bactericidal permeability increasing protein (BPI), elastase,
cathepsin G, myeloperoxidase, proteinase 3, platelet antigens,
myelin protein antigen, islet cell antigen, rheumatoid factor,
histones, ribosomal P proteins, cardiolipin, vimentin, nucleic
acids such as dsDNA, ssDNA, and RNA, ribonuclear particles and
proteins such as Sm antigens (including but not limited to SmD's
and SmB'/B), U1RNP, A2/B1 hnRNP, Ro (SSA), and La (SSB)
antigens.
[0012] In one embodiment, immunoglobulins that bind Fc.gamma.RIIb+
cells and coengage a target antigen on the cell's surface and an
Fc.gamma.RIIb on cell's surface disclosed herein may be variant
immunoglobulins relative to a parent immunoglobulin. In one
embodiment, the variant immunoglobulin comprises a variant Fc
region, wherein said variant Fc region comprises one or more (e.g.,
two or more) modification(s) compared to a parent Fc region,
wherein said modification(s) are at positions selected from the
group consisting of 234, 235, 236, 237, 239, 265, 266, 267, 268,
298, 325, 326, 327, 328, 329, 330, 331, and 332, wherein numbering
is according to the EU index. In one embodiment, the variant
immunoglobulin comprises a variant Fc region, wherein said variant
Fc region comprises one or more (e.g., two or more) modification(s)
compared to a parent Fc region, wherein said modification(s) are at
positions selected from the group consisting of 234, 235, 236, 237,
239, 266, 267, 268, 325, 326, 327, 328, and 332, according to the
EU index. In one embodiment, the variant immunoglobulin comprises a
variant Fc region, wherein said variant Fc region comprises one or
more (e.g., two or more) modification(s) compared to a parent Fc
region, wherein said modification(s) are at positions selected from
the group consisting of 234, 235, 236, 237, 266, 267, 268, 327,
328, according to the EU index. In one embodiment, the variant
immunoglobulin comprises a variant Fc region, wherein said variant
Fc region comprises one or more (e.g., two or more) modification(s)
compared to a parent Fc region, wherein said modification(s) are at
positions selected from the group consisting of 235, 236, 266, 267,
268, 328, according to the EU index. In one embodiment, the variant
immunoglobulin comprises a variant Fc region, wherein said variant
Fc region comprises one or more (e.g., two or more) modification(s)
compared to a parent Fc region, wherein said modification(s) are at
positions selected from the group consisting of 235, 236, 239, 266,
267, 268, and 328, according to the EU index. In one embodiment,
the variant immunoglobulin comprises a variant Fc region, wherein
said variant Fc region comprises one or more (e.g., two or more)
modification(s) compared to a parent Fc region, wherein said
modification(s) are at positions selected from the group consisting
of 234, 235, 236, 237, 266, 267, 268, 327, 328, according to the EU
index
[0013] In one embodiment, said modification(s) is at least one
substitution (e.g., one or more substitution(s), two or more
substitution(s), etc.) selected from the group consisting of 234F,
234G, 234I, 234K, 234N, 234P, 234Q, 234S, 234V, 234W, 234Y, 234D,
234E, 235A, 235E, 235H, 235I, 235N, 235P, 235Q, 235R, 235S, 235W,
235Y, 235D, 235F, 235T, 236D, 236F, 236H, 236I, 236K, 236L, 236M,
236P, 236Q, 236R, 236S, 236T, 236V, 236W, 236Y, 236A, 236E, 236N,
237A, 237E, 237H, 237K, 237L, 237P, 237Q, 237S, 237V, 237Y, 237D,
237N, 239D, 239E, 239N, 239Q, 265E, 266D, 266I, 266M, 267A, 267D,
267E, 267G, 268D, 268E, 268N, 268Q, 298D, 298E, 298L, 298M, 298Q,
325L, 326A, 326E, 326W, 326D, 327D, 327G, 327L, 327N, 327Q, 327E,
328E, 328F, 328Y, 328H, 328I, 328Q, 328W, 329E, 330D, 330H, 330K,
330S, 331S, and 332E, wherein numbering is according to an EU
index. In one embodiment, said modification(s) is at least one
substitution (e.g., one or more substitution(s), two or more
substitution(s), etc.) selected from the group consisting of 234N,
234F, 234D, 234E, 234W, 235Q, 235R, 235W, 235Y, 235D, 235F, 235T,
236D, 236H, 236I, 236L, 236S, 236Y, 236E, 236N, 237H, 237L, 237D,
237N, 239D, 239N, 239E, 266I, 266M, 267A, 267D, 267E, 267G, 268D,
268E, 268N, 268Q, 298E, 298L, 298M, 298Q, 325L, 326A, 326E, 326W,
326D, 327D, 327L, 327E, 328E, 328F, 328Y, 328H, 328I, 328Q, 328W,
330D, 330H, 330K, and 332E, wherein numbering is according to an EU
index. In one embodiment, said modification(s) is at least one
substitution (e.g., one or more substitution(s), two or more
substitution(s), etc.) selected from the group consisting of 234D,
234E, 234W, 235D, 235F, 235R, 235Y, 236D, 236N, 237D, 237N, 239D,
239E, 266M, 267D, 267E, 268D, 268E, 327D, 327E, 328F, 328W, 328Y,
and 332E, wherein numbering is according to an EU index. In one
embodiment, said modification(s) is at least one substitution
(e.g., one or more substitution(s), two or more substitution(s),
etc.) selected from the group consisting of L234E, L235Y, L235R,
G236D, G236N, G237N, V266M, S267E, H268E, H268D, A327D, A327E,
L328F, L328Y, L328W, wherein numbering is according to an EU index.
In one embodiment, said modification(s) is at least one
substitution (e.g., one or more substitution(s), two or more
substitution(s), etc.) selected from the group consisting of 235Y,
236D, 239D, 266M, 267E, 268D, 268E, 328F, 328W, and 328Y, wherein
numbering is according to an EU index. In one embodiment, said
modification(s) is at least one substitution (e.g., one or more
substitution(s), two or more substitution(s), etc.) selected from
the group consisting of L235Y, G236D, V266M, S267E, H268E, H268D,
L328F, L328Y, and L328W, wherein numbering is according to an EU
index.
[0014] In one embodiment, said modification(s) is at least two
modifications (e.g., a combination of modifications) at positions
selected from the group consisting of 234/239, 234/267, 234/328,
235/236, 235/239, 235/267, 235/268, 235/328, 236/239, 236/267,
236/268, 236/328, 237/267, 239/267, 239/268, 239/327, 239/328,
239/332, 266/267, 267/268, 267/325, 267/327, 267/328, 267/332,
268/327, 268/328, 268/332, 326/328, 327/328, and 328/332, wherein
numbering is according to an EU index. In one embodiment, said
modification(s) is at least two modifications (e.g., a combination
of modifications) at positions selected from the group consisting
of 235/267, 236/267, 239/268, 239/267, 267/268, and 267/328,
wherein numbering is according to an EU index. In one embodiment,
said modification(s) is at least two substitutions (e.g., a
combination of substitutions) selected from the group consisting of
234D/267E, 234E/267E, 234F/267E, 234E/328F, 234W/239D, 234W/239E,
234W/267E, 234W/328Y, 235D/267E, 235D/328F, 235F/239D, 235F/267E,
235F/328Y, 235Y/236D, 235Y/239D, 235Y/267D, 235Y/267E, 235Y/268E,
235Y/328F, 236D/239D, 236D/267E, 236D/268E, 236D/328F, 236N/267E,
237D/267E, 237N/267E, 239D/267D, 239D/267E, 239D/268D, 239D/268E,
239D/327D, 239D/328F, 239D/328W, 239D/328Y, 239D/332E, 239E/267E,
266M/267E, 267D/268E, 267E/268D, 267E/268E, 267E/325L, 267E/327D,
267E/327E, 267E/328F, 267E/328I, 267E/328Y, 267E/332E, 268D/327D,
268D/328F, 268D/328W, 268D/328Y, 268D/332E, 268E/328F, 268E/328Y,
327D/328Y, 328F/332E, 328W/332E, and 328Y/332E, wherein numbering
is according to an EU index.
[0015] In one embodiment, said modification(s) result in at least
one of the following substitutions, or combinations of
substitutions: 234F/236N, 234F/236D, 236A/237A, 236S/237A,
235D/239D, 234D/267E, 234E/267E, 234F/267E, 235D/267E, 235F/267E,
235S/267E, 235T/267E, 235Y/267D, 235Y/267E, 236D/267E, 236E/267E,
236N/267E, 237D/267E, 237N/267E, 239D/267D, 239D/267E, 266M/267E,
234E/268D, 236D/268D, 239D/268D, 267D/268D, 267D/268E, 267E/268D,
267E/268E, 267E/325L, 267D/327D, 267D/327E, 267E/327D, 267E/327E,
268D/327D, 239D/328Y, 267E/328F, 267E/328H, 267E/328I, 267E/328Q,
267E/328Y, 268D/328Y, 239D/332E, 328Y/332E, 234D/236N/267E,
235Y/236D/267E, 234W/239E/267E, 235Y/239D/267E, 236D/239D/267E,
235Y/267E/268E, 236D/267E/268E, 239D/267E/268E, 234W/239D/328Y,
235F/239D/328Y, 234E/267E/328F, 235D/267E/328F, 235Y/267E/328F,
236D/267E/328F, 239D/267A/328Y, 239D/267E/328F, 234W/268D/328Y,
235F/268D/328Y, 239D/268D/328F, 239D/268D/328W, 239D/268D/328Y,
239D/268E/328Y, 267A/268D/328Y, 267E/268E/328F, 239D/326D/328Y,
268D/326D/328Y, 239D/327D/328Y, 268D/327D/328Y, 239D/267E/332E,
234W/328Y/332E, 235F/328Y/332E, 239D/328F/332E, 239D/328Y/332E,
267A/328Y/332E, 268D/328F/332E, 268D/328W/332E, 268D/328Y/332E,
268E/328Y/332E, 326D/328Y/332E, 327D/328Y/332E,
234W/236D/239E/267E, 239D/268D/328F/332E, 239D/268D/328W/332E, and
239D/268D/328Y/332E, wherein numbering is according to an EU index.
In one embodiment, said modification(s) result in at least one of
the following substitutions, or combinations of substitutions:
266D, 234F/236N, 234F/236D, 236A/237A, 236S/237A, 235D/239D,
234D/267E, 234E/267E, 234F/267E, 235D/267E, 235F/267E, 235S/267E,
235T/267E, 235Y/267D, 236D/267E, 236E/267E, 236N/267E, 237D/267E,
237N/267E, 266M/267E, 234E/268D, 236D/268D, 267D/268D, 267D/268E,
267E/268D, 267E/268E, 267E/325L, 267D/327D, 267D/327E, 267E/327E,
268D/327D, 239D/328Y, 267E/328F, 267E/328H, 267E/328I, 267E/328Q,
267E/328Y, 268D/328Y, 234D/236N/267E, 235Y/236D/267E,
234W/239E/267E, 235Y/239D/267E, 236D/239D/267E, 235Y/267E/268E,
236D/267E/268E, 234W/239D/328Y, 235F/239D/328Y, 234E/267E/328F,
235D/267E/328F, 235Y/267E/328F, 236D/267E/328F, 239D/267A/328Y,
239D/267E/328F, 234W/268D/328Y, 235F/268D/328Y, 239D/268D/328F,
239D/268D/328W, 239D/268D/328Y, 239D/268E/328Y, 267A/268D/328Y,
267E/268E/328F, 239D/326D/328Y, 268D/326D/328Y, 239D/327D/328Y,
268D/327D/328Y, 234W/328Y/332E, 235F/328Y/332E, 239D/328F/332E,
239D/328Y/332E, 267A/328Y/332E, 268D/328F/332E, 268D/328W/332E,
268D/328Y/332E, 268E/328Y/332E, 326D/328Y/332E, 327D/328Y/332E,
234W/236D/239E/267E, 239D/268D/328F/332E, 239D/268D/328W/332E, and
239D/268D/328Y/332E, wherein numbering is according to an EU index.
In one embodiment, said modification(s) result in at least one of
the following substitutions, or combinations of substitutions:
234N, 235Q, 235R, 235W, 235Y, 236D, 236H, 236I, 236L, 236S, 236Y,
237H, 237L, 239D, 239N, 266I, 266M, 267A, 267D, 267E, 267G, 268D,
268E, 268N, 268Q, 298E, 298L, 298M, 298Q, 326A, 326E, 326W, 327D,
327L, 328E, 328F, 330D, 330H, 330K, 234F/236N, 234F/236D,
235D/239D, 234D/267E, 234E/267E, 234F/267E, 235D/267E, 235F/267E,
235T/267E, 235Y/267D, 235Y/267E, 236D/267E, 236E/267E, 236N/267E,
237D/267E, 237N/267E, 239D/267D, 239D/267E, 266M/267E, 234E/268D,
236D/268D, 239D/268D, 267D/268D, 267D/268E, 267E/268D, 267E/268E,
267E/325L, 267D/327D, 267D/327E, 267E/327D, 267E/327E, 268D/327D,
239D/328Y, 267E/328F, 267E/328H, 267E/328I, 267E/328Q, 267E/328Y,
268D/328Y, 239D/332E, 328Y/332E, 234D/236N/267E, 235Y/236D/267E,
234W/239E/267E, 235Y/239D/267E, 236D/239D/267E, 235Y/267E/268E,
236D/267E/268E, 239D/267E/268E, 234W/239D/328Y, 235F/239D/328Y,
234E/267E/328F, 235D/267E/328F, 235Y/267E/328F, 236D/267E/328F,
239D/267A/328Y, 239D/267E/328F, 234W/268D/328Y, 235F/268D/328Y,
239D/268D/328F, 239D/268D/328W, 239D/268D/328Y, 239D/268E/328Y,
267A/268D/328Y, 267E/268E/328F, 239D/326D/328Y, 268D/326D/328Y,
239D/327D/328Y, 268D/327D/328Y, 239D/267E/332E, 234W/328Y/332E,
235F/328Y/332E, 239D/328F/332E, 239D/328Y/332E, 267A/328Y/332E,
268D/328F/332E, 268D/328W/332E, 268D/328Y/332E, 268E/328Y/332E,
326D/328Y/332E, 327D/328Y/332E, 234W/236D/239E/267E,
239D/268D/328F/332E, 239D/268D/328W/332E, and
239D/268D/328Y/332E
[0016] In one embodiment, said modification(s) result in at least
one of the following substitutions, or combinations of
substitutions: 235Y/267E, 236D/267E, 239D/268D, 239D/267E,
267E/268D, 267E/268E, and 267E/328F, wherein numbering is according
to an EU index.
[0017] In one embodiment, the modifications disclosed herein reduce
affinity to at least one receptor relative to the parent
immunoglobulin, wherein said receptor is selected from the group
consisting of Fc.gamma.RI, Fc.gamma.RIIa, and Fc.gamma.RIIIa. In an
alternate embodiment, immunoglobulin variants disclosed herein
mediate reduced ADCC or ADCP relative to the parent
immunoglobulin.
[0018] Also disclosed herein are methods for engineering the novel
immunoglobulin compositions.
[0019] Also disclosed herein are methods for screening target
antigens for their capacity to mediate cellular inhibition via an
Fc.gamma.RIIb-dependent mechanism. In one embodiment, the antigen
screening methods disclosed herein comprise the step of binding a
cell that expresses the target antigen and Fc.gamma.RIIb with an
immunoglobulin that binds with enhanced affinity, e.g., the Kd of
the immunoglobulin may be less than about 100 nM to at least
Fc.gamma.RIIb. In another embodiment, simultaneous binding of both
target antigen and Fc.gamma.RIIb by the immunoglobulin results in
an inhibitory cellular response. In one embodiment of the screening
methods disclosed herein, the cell is selected from the group
consisting of: B cells, plasma cells, dendritic cells, macrophages,
neutrophils, mast cells, basophils, or eosinophils. In another some
screening methods disclosed herein, the immunoglobulin may be
specific for the target antigen. In an alternate embodiment,
immunoglobulin is specific for an antibody, wherein said antibody
is specific for the target antigen. In an alternate embodiment, the
immunoglobulin is specific for a hapten, and wherein either the
target antigen, or an antibody or protein that is specific for the
target antigen is haptenized.
[0020] Also disclosed herein are isolated nucleic acids encoding
the immunoglobulins described herein. Also disclosed herein are
vectors comprising the nucleic acids, optionally, operably linked
to control sequences. Also disclosed herein are host cells
containing the vectors, and methods for producing and optionally
recovering the immunoglobulin compositions.
[0021] Also disclosed herein are immunoglobulin polypeptides, that
comprise the immunoglobulins disclosed herein. The immunoglobulin
polypeptides may find use in a therapeutic product. In one
embodiment, the immunoglobulin polypeptides disclosed herein may be
antibodies.
[0022] Also disclosed are compositions comprising immunoglobulin
polypeptides described herein, and a physiologically or
pharmaceutically acceptable carrier or diluent.
[0023] Also described are therapeutic and diagnostic uses for the
immunoglobulin polypeptides disclosed herein. In one embodiment,
the immunoglobulins disclosed herein are used to treat one or more
autoimmune disease or inflammatory disease. In an alternate
embodiment, the immunoglobulins disclosed herein are used to treat
one or more hematological malignancies.
BRIEF DESCRIPTION OF THE DRAWINGS
[0024] FIGS. 1A-1B. Alignment of the amino acid sequences of the
human IgG immunoglobulins IgG1, IgG2, IgG3, and IgG4. FIG. 1A
provides the sequences of the CH1 (C.gamma.1) and hinge domains,
and FIG. 1B provides the sequences of the CH2 (C.gamma.2) and CH3
(C.gamma.3) domains. Positions are numbered according to the EU
index of the IgG1 sequence, and differences between IgG1 and the
other immunoglobulins IgG2, IgG3, and IgG4 are shown in gray.
Allotypic polymorphisms exist at a number of positions, and thus
slight differences between the presented sequences and sequences in
the prior art may exist. The possible beginnings of the Fc region
are labeled, defined herein as either EU position 226 or 230.
[0025] FIGS. 2A-2B. Common haplotypes of the human gamma1 (FIG. 2A)
and gamma2 (FIG. 2B) chains.
[0026] FIG. 3. Novel methods of inhibiting B cell activation. Here
CR represents a co-receptor of the BCR complex, but could be any
antigen expressed on any Fc.gamma.RIIb+ cell.
[0027] FIG. 4. Fc.gamma.R positions that contribute to
Fc.gamma.RIIb and Fc.gamma.RIIIa binding selectivity. Positions
were identified by evaluating proximity to the Fc.gamma.R/Fc
interface and amino acid dissimilarity between Fc.gamma.RIIb and
Fc.gamma.RIIIa.
[0028] FIG. 5. Fc positions proximal to Fc.gamma.R positions
contributing to Fc.gamma.RIIb and Fc.gamma.RIIIa binding
selectivity, as listed in FIG. 6.
[0029] FIG. 6. Biacore surface plasmon resonance sensorgrams
showing binding of Fc variant anti-CD19 antibodies to human
Fc.gamma.RIIb.
[0030] FIGS. 7A-7D. Affinities of Fc variant antibodies for human
Fc.gamma.Rs as determined by Biacore surface plasmon resonance.
FIG. 7A is a table listing the dissociation constant (Kd) for
binding anti-CD19 variant antibodies to human Fc.gamma.RI,
Fc.gamma.RIIa (131R), Fc.gamma.RIIa (131H), Fc.gamma.RIIb,
Fc.gamma.RIIIa (158V), and Fc.gamma.RIIIa (158F). FIG. 7B is a
continuation of the list in FIG. 7A. FIG. 7C is a continuation of
the list in FIG. 7A and FIG. 7B. FIG. 7D is a continuation of the
list in FIG. 7A, FIG. 7B, and FIG. 7C. Multiple observations have
been averaged. n.d.=no detectable binding.
[0031] FIGS. 8A-8D. Fold affinities of Fc variant antibodies for
human Fc.gamma.Rs as determined by Biacore surface plasmon
resonance. FIG. 8A is a table listing the fold improvement or
reduction in affinity relative to WT IgG1 for binding of anti-CD19
variant antibodies to human Fc.gamma.RI, Fc.gamma.RIIa (131R),
Fc.gamma.RIIa (131H), Fc.gamma.RIIb, Fc.gamma.RIIIa (158V), and
Fc.gamma.RIIIa (158F). FIG. 8B is a continuation of the list in
FIG. 8A. FIG. 8C is a continuation of the list in FIG. 8A and FIG.
8B.
[0032] FIG. 8D is a continuation of the list in FIG. 8A, FIG. 8B,
and FIG. 8C. Fold=KD(Native IgG1)/KD(variant). n.d.=no detectable
binding.
[0033] FIG. 9. Affinities of Fc variant antibodies for human
Fc.gamma.Rs as determined by Biacore surface plasmon resonance. The
graph shows the -log(KD) for binding of anti-CD19 variant and WT
IgG1 antibodies to human Fc.gamma.RI (I), R131 Fc.gamma.RIIa
(RIIa), H131 Fc.gamma.RIIa (HIIa), Fc.gamma.RIIb (IIb), and V158
Fc.gamma.RIIIa (VIIIa). Binding of L235Y/S267E, G236D/S267E, and
S267E/L328F to V158 Fc.gamma.RIIIa was not detectable.
[0034] FIG. 10. Affinities of Fc variant antibodies for human
Fc.gamma.Rs as determined by Biacore surface plasmon resonance. The
graph shows the -log(KD) for binding of anti-CD19 variant and WT
IgG1 antibodies to human Fc.gamma.RI (I), R131 Fc.gamma.RIIa
(RIIa), H131 Fc.gamma.RIIa (HIIa), Fc.gamma.RIIb (IIb), and V158
Fc.gamma.RIIIa (VIIIa).
[0035] FIGS. 11A-11E. Analysis of combination variants (doubles,
triples) for synergistic and non-additive effects in binding to
human Fc.gamma.RIIb (FIG. 11A), Fc.gamma.RI (FIG. 11B), R131
Fc.gamma.RIIa (FIG. 11C), H131 Fc.gamma.RIIa (FIG. 11D), and V158
Fc.gamma.RIIIa (FIG. 11E). The ratio between actual fold
improvement measured by SPR and expected fold improvement
calculated by multiplying the fold improvements of the single
substitution variants is plotted. Ratios greater than one indicate
a synergistic effect.
[0036] FIG. 12. Binding of Fc variant antibodies to human
Fc.gamma.Rs relative to WT IgG1 as measured by cell surface
binding. Antibodies (variant and WT IgG1) were added to HEK293T
cells transfected with Fc.gamma.RIIb to assess cell surface
binding. The binding curves were constructed by plotting MFI as a
function of Fc variant concentration.
[0037] FIGS. 13A-13D. Affinities of Fc variant antibodies for mouse
and cynologous monkey (Macaca fascicularis) Fc.gamma.Rs as
determined by Biacore surface plasmon resonance, either by
dissociation constant (Kd) or off-rate determination as indicated.
FIG. 13A is a table listing the fold improvement relative to WT
IgG1 for binding of anti-CD19 antibody variants to mouse
Fc.gamma.RI, mouse Fc.gamma.RII, mouse Fc.gamma.RII, mouse
Fc.gamma.RIV, cynomolgus monkey Fc.gamma.RI, cynomolgus monkey
Fc.gamma.RIIa, cynomolgus monkey Fc.gamma.RIIb, and cynomolgus
monkey Fc.gamma.RIIIa. FIG. 13B is a continuation of the list in
FIG. 13A. FIG. 13C is a continuation of the list in FIG. 13A and
FIG. 13B.
[0038] FIG. 13D is a continuation of the list in FIG. 13A, FIG.
13B, and FIG. 13C. NB=no detectable binding.
[0039] FIG. 14. Affinities of Fc variant antibodies for human
Fc.gamma.Rs as determined by Biacore surface plasmon resonance. The
graph shows the -log(KD) for binding of anti-CD19 variant and WT
IgG1 antibodies to human Fc.gamma.RI (I), R131 Fc.gamma.RIIa
(RIIa), H131 Fc.gamma.RIIa (HIIa), Fc.gamma.RIIb (IIb), and V158
Fc.gamma.RIIIa (VIIIa).
[0040] FIGS. 15A-15B. ATP-dependent B cell viability assay
demonstrating the survival of primary human B cells upon BCR
activation, here carried out by crosslinking with anti-mu (FIG.
15A) or anti-CD79b (FIG. 15B) antibodies.
[0041] FIG. 16. Inhibition of B cell proliferation by Fc variant
anti-CD19 antibodies. Anti-RSV (Respiratory Syncytial Virus)
S267E/L328F is used as a control (RSV is not expressed on B cells).
An ATP-dependent luminescence assay was used to measure B cell
proliferation in the presence of 10 .mu.g/ml anti-CD79b activating
antibody, and the effect of anti-CD19-S267E/L328F was compared to
anti-CD19-IgG1 (native IgG1 Fv control) and anti-RSV-S267E/L328F
(non-CD19 Fc control). To assess the importance of CD19 and
Fc.gamma.RIIb coengagement, anti-RSV-S267E/L328F alone or in
combination with anti-CD19-IgG1 was used.
[0042] FIG. 17. Inhibition of B cell proliferation by Fc variant
anti-CD19 antibodies. An ATP-dependent luminescence assay was used
to measure proliferation of primary human B cells in the presence
of 1 .mu.g/ml anti-CD79b activating antibody, and varying
concentrations of the indicated anti-CD19 or anti-RSV control
antibodies.
[0043] FIG. 18. Inhibition of B cell proliferation by Fc variant
anti-CD19 antibodies. An ATP-dependent luminescence assay was used
to measure proliferation of primary human B cells in the presence
of 2 .mu.g/ml anti-.mu. (mu) antibody and varying concentrations of
the indicated anti-CD19 antibodies.
[0044] FIG. 19. Coengagement of Fc.gamma.RIIb and CD19 by IIbE
variants inhibits BCR activation-induced calcium mobilization in
primary human B cells. Calcium mobilization was induced with 10
.mu.g/ml anti-CD79b BCR-activating antibody. Calcium mobilization
was measured in the presence of 10 .mu.g/ml fixed concentration of
anti-CD19 IIbE variants, .alpha.-CD19-IgG1 (native IgG1 Fv
control), .alpha.-FITC-S267E/L328F (non-CD19 Fc control), or PBS
vehicle. The data are plotted as the change of MFI over time, or
the area under the response curve normalized to the maximum
measured signal intensity.
[0045] FIG. 20. Coengagement of Fc.gamma.RIIb and CD19 by IIbE
variants inhibits BCR activation-induced calcium mobilization in
primary human B cells. Calcium mobilization was induced with 10
.mu.g/ml anti-CD79b BCR-activating antibody. Calcium mobilization
was measured at multiple antibody concentrations for anti-CD19-IgG1
and three IIbE variants, and the areas under the curves were
plotted to obtain dose-response relationships.
[0046] FIG. 21. Correlation between affinity for Fc.gamma.RIIb and
inhibition of calcium release. EC50 data are from FIG. 20, and
symbols are the same as indicated in FIG. 22. Affinities are from
Biacore data presented in FIG. 7A-FIG. 7D.
[0047] FIG. 22. Coengagement of Fc.gamma.RIIb and CD19 by IIbE
variants inhibits BCR activation-induced calcium mobilization in
primary human B cells. Calcium mobilization was induced with 10
.mu.g/ml anti-CD79b BCR-activating antibody. Soluble Fc.gamma.RI
(50 .quadrature..mu.g/ml) added to 10 .mu.g/ml
.alpha.-CD19-S267E/L328F completely abolished the IIbE variant's
inhibitory effect on calcium mobilization, confirming the
importance of Fc.gamma.RIIb engagement by anti-CD19 antibody.
[0048] FIG. 23. IIbE variant anti-CD19-S267E/L328F activates
Fc.gamma.RIIb-mediated SHIP phosphorylation in primary human B
cells. Anti-CD19-S267E/L328F, anti-CD19-IgG1 (Fv control),
anti-RSV-S267E/L328F (Fc control), anti-CD19-Fc KO (Fv control), or
anti-Fc.gamma.RII (10 .mu.g/ml each) were added to B cells in the
presence of 20 .mu.g/ml anti-CD79b antibody. As a positive control,
20 .mu.g/ml goat anti-mouse IgG F(ab')2 fragment was used to
crosslink anti-CD79b and anti-Fc.gamma.RII antibodies. A blot of
total cellular extracts was probed with anti-pSHIP, with anti-GAPDH
used as a loading control. Relative to negative controls,
anti-CD19-S267E/L328F induced greater SHIP1 phosphorylation than
direct crosslinking of BCR and Fc.gamma.RIIb by CD79b and
Fc.gamma.RIIb antibodies.
[0049] FIG. 24. Anti-CD19-S267E/L328F inhibits the anti-apoptotic
effects of BCR activation on primary human B cells. Inhibition of
BCR-mediated survival signals by Fc.gamma.RIIb and CD19
coengagement was examined using annexin-V staining in the presence
of 10 .mu.g/ml anti-CD79b. B cell apoptosis was stimulated by
anti-CD19-S267E/L328F, but not anti-CD19-IgG1 (Fv control),
anti-RSV-S267E/L328F (Fc control), or the two controls
combined.
[0050] FIG. 25. NK-cell mediated ADCC activity of Fc variant
antibodies against Ramos B cells.
[0051] FIG. 26. Macrophage mediated phagocytosis (ADCP) activity of
Fc variant antibodies against RS4;11 B cells.
[0052] FIG. 27. Fc variant anti-CD19 antibodies do not mediate CDC
activity against Raji B cells.
[0053] FIGS. 28A-28B. Evaluation of the capacity of co-engagement
of CD19 and Fc.gamma.RIIb to inhibit human B cell activation in
vivo. (FIG. 28A) Schematic representation of the experimental
protocol. (FIG. 28B) Titer of anti-tetanus toxoid (TT) specific
antibody in huPBL-SCID mice after TT immunization and treatment
with vehicle (PBS), anti-CD19 IgG1 WT, anti-CD19 with enhanced
Fc.gamma.RIIb affinity (.alpha.-CD19 S267E/L328F), or anti-CD20
(Rituximab).
[0054] FIG. 29. Target antigens that may be effective Fc.gamma.RIIb
co-targets for modulation of cellular activity. B=B cells,
Plasma=plasma cells, DC=dendritic cells, M.PHI.=macrophages,
PMN=neutrophils, Baso=basophils, Eos=eosinophils, and Mast=mast
cells.
[0055] FIG. 30. ATP-dependent luminescence assay measuring B cell
proliferation in the presence of 1 .mu.g/ml
anti-CD79b-SN8-G236R/L328R antibody, and varying concentrations of
either enhanced Fc.gamma.RIIb variant (S267E/L328F) or Fc.gamma.R
knockout variant (G236R/L328R or 236R/L328R) versions of anti-CD20
(clone PR070769), -CD52 (Campath), and -CD19 (HuAM4G7)
antibodies.
[0056] FIG. 31. ATP-dependent luminescence assay measuring B cell
proliferation in the presence of 2 .mu.g/ml anti-.mu. antibody, and
varying concentrations of either enhanced Fc.gamma.RIIb variant
(S267E/L328F), Fc.gamma.R knockout variant (G236R/L328R), or WT
IgG1 versions of anti-CD23 antibodies (clone 5E8 or C11).
[0057] FIG. 32. ATP-dependent luminescence assay measuring B cell
proliferation in the presence of 2 .mu.g/ml anti-.mu. antibody, and
either enhanced Fc.gamma.RIIb variant (S267E/L328F), Fc.gamma.R
knockout variant (G236R/L328R), or WT IgG1 versions of the
anti-CD79b antibody SN8.
[0058] FIG. 33. ATP-dependent luminescence assay measuring B cell
proliferation in the presence of 2 .mu.g/ml anti-.mu. antibody, and
varying concentrations of either enhanced Fc.gamma.RIIb variant
(S267E/L328F), Fc.gamma.R knockout variant (G236R/L328R), or WT
IgG1 versions of anti-CD22 antibody.
[0059] FIGS. 34A-34B. ATP-dependent luminescence assay measuring B
cell proliferation in the presence of BCR stimulation by (FIG. 34A)
1 .mu.g/ml anti-CD79b-SN8-G236R/L328R antibody or (FIG. 34B) 2
.mu.g/ml anti-.mu. antibody, and varying concentrations of either
enhanced Fc.gamma.RIIb variant (S267E/L328F), Fc.gamma.R knockout
variant (G236R/L328R), or WT IgG1 versions of anti-CD40 antibodies
(clones PFCD40, S2C6, G28.5, and 5D12).
[0060] FIG. 35. ATP-dependent luminescence assay measuring B cell
proliferation in the presence of 1 .mu.g/ml
anti-CD79b-SN8-G236R/L328R antibody, and varying concentrations of
either enhanced Fc.gamma.RIIb variant (S267E/L328F) or Fc.gamma.R
knockout variant (G236R/L328R or 236R/L328R) versions of anti-CD19
antibodies (clones HD37, 21D4, or HuAM4G7.
[0061] FIGS. 36A-36D. Calcium release assay measuring inhibition
capacity of variant antibodies with specificity for CD22 (FIG.
36A), CD23 (FIG. 36B), CD40 (FIG. 36C), and CD79b (FIG. 36D).
Calcium mobilization was induced with 10 .mu.g/ml
anti-CD79b-SN8-G236R/L328R antibody, and monitored in the presence
of either enhanced Fc.gamma.RIIb variant (S267E/L328F) or
Fc.gamma.R knockout variant (G236R/L328R) versions of anti-CD22,
-CD23, -CD40, and CD79b antibodies.
[0062] FIG. 37. Hapten approach to screening target antigens for
capacity to modulate cellular activity upon high affinity
co-targeting with Fc.gamma.RIIb.
[0063] FIG. 38. ATP-dependent luminescence assay measuring B cell
proliferation in the presence of 2 .mu.g/ml FITCylated anti-.mu.
F(ab')2 and varying concentrations of either enhanced Fc.gamma.RIIb
variant (S267E/L328F), Fc.gamma.R knockout variant (G236R/L328R or
236R/L328R), or WT IgG1 versions of anti-FITC antibody (clone
4-4-20). Anti-RSV was included as a control.
[0064] FIG. 39. ATP-dependent luminescence assay measuring B cell
proliferation in the presence of 2 .mu.g/ml anti-.mu. F(ab')2, 0.5
.mu.g/ml FITC-labeled anti-CD19 (clone murine 4G7 IgG1), and
varying concentrations of either enhanced Fc.gamma.RIIb variant
(S267E/L328F), Fc.gamma.R knockout variant ( 236R/L328R), or WT
IgG1 versions of anti-FITC antibody.
[0065] FIGS. 40A-40B. ATP-dependent luminescence assay measuring B
cell proliferation in the presence of 2 .mu.g/ml anti-.mu. F(ab')2,
0.5 .mu.g/ml FITC-labeled anti-CD20 clone PDR-79 (FIG. 40A) or 1
.mu.g/ml FITC-labeled Rituxan (FIG. 40B), and varying
concentrations of either enhanced Fc.gamma.RIIb variant
(S267E/L328F), Fc.gamma.R knockout variant ( 236R/L328R), or WT
IgG1 versions of anti-FITC antibody. FITC-labeled anti-mu at 2
.mu.g/ml is also included in (FIG. 40B) as a control.
[0066] FIG. 41. ATP-dependent luminescence assay measuring B cell
proliferation in the presence of 1 .mu.g/ml anti-CD79b (SN8)
antibody, 0.5 .mu.g/ml FITC-labeled anti-CD21, and varying
concentrations of either enhanced Fc.gamma.RIIb variant
(S267E/L328F) or WT IgG1 versions of anti-FITC antibody.
[0067] FIG. 42. ATP-dependent luminescence assay measuring B cell
proliferation in the presence of 1 .mu.g/ml anti-CD79b (SN8)
antibody, 0.5 .mu.g/ml FITC-labeled anti-CD24, and varying
concentrations of either enhanced Fc.gamma.RIIb variant
(S267E/L328F) or WT IgG1 versions of anti-FITC antibody.
[0068] FIG. 43. ATP-dependent luminescence assay measuring B cell
proliferation in the presence of 2 .mu.g/ml anti-.mu. F(ab')2, 0.25
.mu.g/ml FITC-labeled anti-CD1 or 0.5 .mu.g/ml FITC-labeled
anti-CD24, and varying concentrations of either enhanced
Fc.gamma.RIIb variant (S267E/L328F) or WT IgG1 versions of
anti-FITC antibody.
[0069] FIG. 44. ATP-dependent luminescence assay measuring B cell
proliferation in the presence of 1 .mu.g/ml anti-CD79b (SN8)
antibody, FITC-labeled anti-CD35, and varying concentrations of
either enhanced Fc.gamma.RIIb variant (S267E/L328F) or Fc.gamma.R
knockout (G236R/L328R) versions of anti-FITC antibody.
[0070] FIG. 45. ATP-dependent luminescence assay measuring B cell
proliferation in the presence of 1 .mu.g/ml anti-CD79b (SN8)
antibody, FITC-labeled anti-CD45RA, and varying concentrations of
either enhanced Fc.gamma.RIIb variant (S267E/L328F) or Fc.gamma.R
knockout (G236R/L328R) versions of anti-FITC antibody.
[0071] FIG. 46. ATP-dependent luminescence assay measuring B cell
proliferation in the presence of 1 .mu.g/ml anti-CD79b (SN8)
antibody, FITC-labeled anti-CD72, and varying concentrations of
either enhanced Fc.gamma.RIIb variant (S267E/L328F) or Fc.gamma.R
knockout (G236R/L328R) versions of anti-FITC antibody.
[0072] FIG. 47. ATP-dependent luminescence assay measuring B cell
proliferation in the presence of 2 .mu.g/ml anti-.mu. F(ab')2, 2
.mu.g/ml FITC-labeled anti-CD79a (clone ZL7-4), and varying
concentrations of either enhanced Fc.gamma.RIIb variant
(S267E/L328F), Fc.gamma.R knockout ( 236R/L328R) or WT IgG1
versions of anti-FITC antibody.
[0073] FIG. 48. ATP-dependent luminescence assay measuring B cell
proliferation in the presence of 2 .mu.g/ml anti-.mu. F(ab')2, 1.8
.mu.g/ml FITC-labeled anti-CD79b (clone ZL9-3), and varying
concentrations of either enhanced Fc.gamma.RIIb variant
(S267E/L328F), Fc.gamma.R knockout ( 236R/L328R) or WT IgG1
versions of anti-FITC antibody.
[0074] FIG. 49. ATP-dependent luminescence assay measuring B cell
proliferation in the presence of 1 .mu.g/ml anti-CD79b (SN8)
antibody, FITC-labeled anti-CD80, and varying concentrations of
either enhanced Fc.gamma.RIIb variant (S267E/L328F) or Fc.gamma.R
knockout (G236R/L328R) versions of anti-FITC antibody.
[0075] FIG. 50. ATP-dependent luminescence assay measuring B cell
proliferation in the presence of FITC-labeled anti-CD81, varying
concentrations of either enhanced Fc.gamma.RIIb variant
(S267E/L328F), Fc.gamma.R knockout variant (G236R/L328R), or WT
IgG1 versions of anti-FITC antibody, and 2 .mu.g/ml anti-.mu.
antibody.
[0076] FIG. 51. ATP-dependent luminescence assay measuring B cell
proliferation in the presence of 1 .mu.g/ml
anti-CD79b(SN8)-G236R/L328R antibody, FITC-labeled anti-CD86, and
varying concentrations of either enhanced Fc.gamma.RIIb variant
(S267E/L328F) or Fc.gamma.R knockout (G236R/L328R) versions of
anti-FITC antibody.
[0077] FIG. 52. ATP-dependent luminescence assay measuring B cell
proliferation in the presence of 1 .mu.g/ml
anti-CD79b(SN8)-G236R/L328R antibody, FITC-labeled anti-HLA-DR, and
varying concentrations of either enhanced Fc.gamma.RIIb variant
(S267E/L328F) or Fc.gamma.R knockout (G236R/L328R) versions of
anti-FITC antibody.
[0078] FIG. 53. Summary of results from target antigen screening
for capacity of antigens to modulate B cell activation when
co-targeted with high affinity Fc.gamma.RIIb binding. Results are
from the ATP-dependence luminscence B cell viability assay or
calcium mobilization assay using either Fc variant versions of
antibodies with specificity for the indicated target antigens (Fc
variant approach) or Fc variant versions of the anti-FITC antibody
together with commercial antibodies with specificity for the
indicated target antigens (Hapten approach)
[0079] FIGS. 54A-54D. FIG. 54A lists the amino acid sequences of
various variable regions, heavy chain constant regions, and full
length antibodies. FIG. 54B is a continuation of the list in FIG.
54A. FIG. 54C is a continuation of the list in FIG. 54A and FIG.
54B. FIG. 54D is a continuation of the list in FIG. 54A, FIG. 54B,
and FIG. 54C.
DETAILED DESCRIPTION OF EXEMPLARY EMBODIMENTS
[0080] The humoral immune response (e.g., the result of diverse B
cell responses) may be initiated when B cells are activated by an
antigen and subsequently differentiated into plasma cells. Binding
of membrane bound B cell receptor (BCR) on B cells by an antigen
activates an intracellular signaling cascade, including calcium
mobilization, which leads to cell proliferation and
differentiation. Coengagement of cognate BCR) with the inhibitory
Fc receptor (Fc.gamma.RIIb) inhibits B cell activation signals
through a negative feedback loop.
[0081] The importance of Fc.gamma.RIIb in negative regulation of B
cell responses has been demonstrated using Fc.gamma.RIIb-deficient
mice, which fail to regulate humoral responses (Wernersson, S. et
al., 1999, J. Immunol. 163, 618-622), are sensitized to
collagen-induced arthritis (Yuasa, T. et al., 1999, J. Exp. Med.
189, 187-194), and develop lupus-like disease (Fukuyama, H. et al.,
J. V., 2005, Nat. Immunol. 6, 99-106; McGaha, T. L. et al., 2005,
Science 307, 590-593) and Goodpasture's syndrome (Nakamura, A. et
al., 2000, J. Exp. Med. 191, 899-906). Fc.gamma.RIIb dysregulation
has also been associated with human autoimmune disease. For
example, polymorphisms in the promoter (Blank, M. C. et al., 2005,
Hum. Genet. 117, 220-227; Olferiev, M. et al., 2007, J. Biol. Chem.
282, 1738-1746) and transmembrane domain (Chen, J. Y. et al., 2006,
Arthritis Rheum. 54, 3908-3917; Floto, R. A. et al., Nat. Med. 11,
1056-1058; Li, X. et al., 2003, Arthritis Rheum. 48, 3242-3252) of
Fc.gamma.RIIb have been linked with increased prevalence of
systemic lupus erythematosus (SLE). SLE patients also show reduced
Fc.gamma.RIIb surface expression on B cells (Mackay, M. et al.,
2006, J. Exp. Med. 203, 2157-2164; Su, K. et al., 2007, J. Immunol.
178, 3272-3280) and, as a consequence, exhibit dysregulated calcium
signaling (Mackay, M. et al., 2006, J. Exp. Med. 203, 2157-2164).
The pivotal role of Fc.gamma.RIIb in regulating B cells, supported
by mouse models and clinical evidence, makes it an attractive
therapeutic target for controlling autoimmune and inflammatory
disorders (Pritchard, N. R. & Smith, K. G., 2003, Immunology
108, 263-273; Ravetch, J. V. & Lanier, L. L., 2000, Science
290, 84-89; Stefanescu, R. N. et al., 2004, J. Clin. Immunol. 24,
315-326).
[0082] Described herein are antibodies that mimic the inhibitory
effects of coengagement of cognate BCR with Fc.gamma.RIIb on B
cells. For example, describe herein are variant anti-CD19
antibodies engineered such that the Fc domain binds to
Fc.gamma.RIIb with up to 430-fold greater affinity. Relative to
native IgG1, the Fc.gamma.RIIb binding-enhanced (IIbE) variants
strongly inhibit BCR-induced calcium mobilization and viability in
primary human B cells. Inhibitory effects involved phosphorylation
of SH2-containing inositol polyphosphate 5-phosphatase (SHIP),
which is known to be involved in Fc.gamma.RIIb-induced negative
feedback of B cell activation. Coengagement of BCR and
Fc.gamma.RIIb by IIbE variants also overcame the anti-apoptotic
effects of BCR activation. The use of a single antibody to suppress
B cell functions by coengagement of cognate BCR and Fc.gamma.RIIb
may represent a novel approach in the treatment of B cell-mediated
diseases. Nonlimiting examples of B cell-mediated diseases include
hematological malignancies, autoimmunity, allergic responses,
etc.
[0083] Described herein are several definitions. Such definitions
are meant to encompass grammatical equivalents.
[0084] By "ADCC" or "antibody dependent cell-mediated cytotoxicity"
as used herein is meant the cell-mediated reaction wherein
nonspecific cytotoxic cells that express Fc.gamma.Rs recognize
bound antibody on a target cell and subsequently cause lysis of the
target cell.
[0085] By "ADCP" or antibody dependent cell-mediated phagocytosis
as used herein is meant the cell-mediated reaction wherein
nonspecific cytotoxic cells that express Fc.gamma.Rs recognize
bound antibody on a target cell and subsequently cause phagocytosis
of the target cell.
[0086] By "antibody" herein is meant a protein consisting of one or
more polypeptides substantially encoded by all or part of the
recognized immunoglobulin genes. The recognized immunoglobulin
genes, for example in humans, include the kappa (.kappa.), lambda
(.lamda.), and heavy chain genetic loci, which together comprise
the myriad variable region genes, and the constant region genes mu
(.upsilon.), delta (.delta.), gamma (.gamma.), sigma (.sigma.), and
alpha (.alpha.) which encode the IgM, IgD, IgG (IgG1, IgG2, IgG3,
and IgG4), IgE, and IgA (IgA1 and IgA2) isotypes respectively.
Antibody herein is meant to include full length antibodies and
antibody fragments, and may refer to a natural antibody from any
organism, an engineered antibody, or an antibody generated
recombinantly for experimental, therapeutic, or other purposes.
[0087] By "amino acid" and "amino acid identity" as used herein is
meant one of the 20 naturally occurring amino acids or any
non-natural analogues that may be present at a specific, defined
position.
[0088] By "CD32b+ cell" or "Fc.gamma.RIIb.sup.+ cell" as used
herein is meant any cell or cell type that expresses CD32b
(Fc.gamma.RIIb). CD32b+ cells include but are not limited to B
cells, plasma cells, dendritic cells, macrophages, neutrophils,
mast cells, basophils, or eosinophils.
[0089] By "CDC" or "complement dependent cytotoxicity" as used
herein is meant the reaction wherein one or more complement protein
components recognize bound antibody on a target cell and
subsequently cause lysis of the target cell.
[0090] By "constant region" of an antibody as defined herein is
meant the region of the antibody that is encoded by one of the
light or heavy chain immunoglobulin constant region genes. By
"constant light chain" or "light chain constant region" as used
herein is meant the region of an antibody encoded by the kappa
(C.kappa.) or lambda (C.lamda.) light chains. The constant light
chain typically comprises a single domain, and as defined herein
refers to positions 108-214 of C.kappa. or C.lamda., wherein
numbering is according to the EU index. By "constant heavy chain"
or "heavy chain constant region" as used herein is meant the region
of an antibody encoded by the mu, delta, gamma, alpha, or epsilon
genes to define the antibody's isotype as IgM, IgD, IgG, IgA, or
IgE, respectively. For full length IgG antibodies, the constant
heavy chain, as defined herein, refers to the N-terminus of the CH1
domain to the C-terminus of the CH3 domain, thus comprising
positions 118-447, wherein numbering is according to the EU
index.
[0091] By "effector function" as used herein is meant a biochemical
event that results from the interaction of an antibody Fc region
with an Fc receptor or ligand. Effector functions include
Fc.gamma.R-mediated effector functions such as ADCC and ADCP, and
complement-mediated effector functions such as CDC. Further,
effector functions include Fc.gamma.RIIb-mediated effector
functions, such as inhibitory functions (e.g., downregulating,
reducing, inhibiting etc., B cell responses, e.g., a humoral immune
response).
[0092] By "effector cell" as used herein is meant a cell of the
immune system that expresses one or more Fc and/or complement
receptors and mediates one or more effector functions.
[0093] Effector cells include but are not limited to monocytes,
macrophages, neutrophils, dendritic cells, eosinophils, mast cells,
platelets, B cells, large granular lymphocytes, Langerhans' cells,
natural killer (NK) cells, and .gamma..delta. T cells, and may be
from any organism including but not limited to humans, mice, rats,
rabbits, and monkeys.
[0094] By "Fab" or "Fab region" as used herein is meant the
polypeptides that comprise the V.sub.H, CH1, V.sub.H, and C.sub.L
immunoglobulin domains. Fab may refer to this region in isolation,
or this region in the context of a full length antibody or antibody
fragment.
[0095] By "Fc" or "Fc region", as used herein is meant the
polypeptide comprising the constant region of an antibody excluding
the first constant region immunoglobulin domain and in some cases,
part of the hinge. Thus Fc refers to the last two constant region
immunoglobulin domains of IgA, IgD, and IgG, and the last three
constant region immunoglobulin domains of IgE and IgM, and the
flexible hinge N-terminal to these domains. For IgA and IgM, Fc may
include the J chain. For IgG, Fc comprises immunoglobulin domains
Cgamma2 and Cgamma3 (C.gamma.2 and C.gamma.3) and the hinge between
Cgamma1 (C.gamma.1) and Cgamma2 (C.gamma.2). Although the
boundaries of the Fc region may vary, the human IgG heavy chain Fc
region is usually defined to comprise residues C226 or P230 to its
carboxyl-terminus, wherein the numbering is according to the EU
index as in Kabat. Fc may refer to this region in isolation, or
this region in the context of an Fc polypeptide, as described
below.
[0096] By "Fc polypeptide" as used herein is meant a polypeptide
that comprises all or part of an Fc region. Fc polypeptides include
antibodies, Fc fusions, isolated Fcs, and Fc fragments.
Immunoglobulins may be Fc polypeptides.
[0097] By "Fc fusion" as used herein is meant a protein wherein one
or more polypeptides is operably linked to Fc. Fc fusion is herein
meant to be synonymous with the terms "immunoadhesin", "Ig fusion",
"Ig chimera", and "receptor globulin" (sometimes with dashes) as
used in the prior art (Chamow et al., 1996, Trends Biotechnol
14:52-60; Ashkenazi et al., 1997, Curr Opin Immunol 9:195-200, both
hereby entirely incorporated by reference). An Fc fusion combines
the Fc region of an immunoglobulin with a fusion partner, which in
general may be any protein, polypeptide or small molecule. The role
of the non-Fc part of an Fc fusion, i.e., the fusion partner, is to
mediate target binding, and thus it is functionally analogous to
the variable regions of an antibody. Virtually any protein or small
molecule may be linked to Fc to generate an Fc fusion. Protein
fusion partners may include, but are not limited to, the
target-binding region of a receptor, an adhesion molecule, a
ligand, an enzyme, a cytokine, a chemokine, or some other protein
or protein domain. Small molecule fusion partners may include any
therapeutic agent that directs the Fc fusion to a therapeutic
target. Such targets may be any molecule, e.g., an extracellular
receptor that is implicated in disease.
[0098] By "Fc gamma receptor" or "Fc.gamma.R" as used herein is
meant any member of the family of proteins that bind the IgG
antibody Fc region and are substantially encoded by the Fc.gamma.R
genes. In humans this family includes but is not limited to
Fc.gamma.RI (CD64), including isoforms Fc.gamma.RIa, Fc.gamma.RIb,
and Fc.gamma.RIc; Fc.gamma.RII (CD32), including isoforms
Fc.gamma.RIIa (including allotypes H131 and R131), Fc.gamma.RIIb
(including Fc.gamma.RIIb-1 and Fc.gamma.RIIb-2), and Fc.gamma.RIIc;
and Fc.gamma.RIII (CD16), including isoforms Fc.gamma.RIIIa
(including allotypes V158 and F158) and Fc.gamma.RIIIb (including
allotypes Fc.gamma.RIIIb-NA1 and Fc.gamma.RIIIb-NA2) (Jefferis et
al., 2002, Immunol Lett 82:57-65, incorporated entirely by
reference), as well as any undiscovered human Fc.gamma.Rs or
Fc.gamma.R isoforms or allotypes. An Fc.gamma.R may be from any
organism, including but not limited to humans, mice, rats, rabbits,
and monkeys. Mouse Fc.gamma.Rs include but are not limited to
Fc.gamma.RI (CD64), Fc.gamma.RII (CD32), Fc.gamma.RIII (CD16), and
Fc.gamma.RIII-2 (CD16-2), as well as any undiscovered mouse
Fc.gamma.Rs or Fc.gamma.R isoforms or allotypes.
[0099] By "Fc ligand" or "Fc receptor" as used herein is meant a
molecule, e.g., a polypeptide, from any organism that binds to the
Fc region of an antibody to form an Fc-ligand complex. Fc ligands
include but are not limited to Fc.gamma.Rs, Fc.gamma.Rs,
Fc.gamma.Rs, FcRn, C1q, C3, mannan binding lectin, mannose
receptor, staphylococcal protein A, streptococcal protein G, and
viral Fc.gamma.R. Fc ligands also include Fc receptor homologs
(FcRH), which are a family of Fc receptors that are homologous to
the Fc.gamma.Rs (Davis et al., 2002, Immunological Reviews
190:123-136). Fc ligands may include undiscovered molecules that
bind Fc.
[0100] By "full length antibody" as used herein is meant the
structure that constitutes the natural biological form of an
antibody, including variable and constant regions. For example, in
most mammals, including humans and mice, the full length antibody
of the IgG isotype is a tetramer and consists of two identical
pairs of two immunoglobulin chains, each pair having one light and
one heavy chain, each light chain comprising immunoglobulin domains
VL and CL, and each heavy chain comprising immunoglobulin domains
VH, C.gamma.1, C.gamma.2, and C.gamma.3. In some mammals, for
example in camels and llamas, IgG antibodies may consist of only
two heavy chains, each heavy chain comprising a variable domain
attached to the Fc region.
[0101] By "immunoglobulin" herein is meant a protein comprising one
or more polypeptides substantially encoded by immunoglobulin genes.
Immunoglobulins include but are not limited to antibodies
(including bispecific antibodies) and Fc fusions. Immunoglobulins
may have a number of structural forms, including but not limited to
full length antibodies, antibody fragments, and individual
immunoglobulin domains.
[0102] By "immunoglobulin (Ig) domain" as used herein is meant a
region of an immunoglobulin that exists as a distinct structural
entity as ascertained by one skilled in the art of protein
structure. Ig domains typically have a characteristic
.beta.-sandwich folding topology. The known Ig domains in the IgG
isotype of antibodies are VH C.gamma.1, C.gamma.2, C.gamma.3, VL,
and CL.
[0103] By "IgG" or "IgG immunoglobulin" as used herein is meant a
polypeptide belonging to the class of antibodies that are
substantially encoded by a recognized immunoglobulin gamma gene. In
humans this class comprises the subclasses or isotypes IgG1, IgG2,
IgG3, and IgG4. By "isotype" as used herein is meant any of the
subclasses of immunoglobulins defined by the chemical and antigenic
characteristics of their constant regions. The known human
immunoglobulin isotypes are IgG1, IgG2, IgG3, IgG4, IgA1, IgA2,
IgM, IgD, and IgE.
[0104] By "modification" herein is meant an alteration in the
physical, chemical, or sequence properties of a protein,
polypeptide, antibody, or immunoglobulin. Modifications described
herein include amino acid modifications and glycoform
modifications.
[0105] By "amino acid modification" herein is meant an amino acid
substitution, insertion, and/or deletion in a polypeptide sequence.
By "amino acid substitution" or "substitution" herein is meant the
replacement of an amino acid at a particular position in a parent
polypeptide sequence with another amino acid. For example, the
substitution S267E refers to a variant polypeptide, in this case a
constant heavy chain variant, in which the serine at position 267
is replaced with glutamic acid. By "amino acid insertion" or
"insertion" as used herein is meant the addition of an amino acid
at a particular position in a parent polypeptide sequence. By
"amino acid deletion" or "deletion" as used herein is meant the
removal of an amino acid at a particular position in a parent
polypeptide sequence.
[0106] By "glycoform modification" or "modified glycoform" or
"engineered glycoform" as used herein is meant a carbohydrate
composition that is covalently attached to a protein, for example
an antibody, wherein said carbohydrate composition differs
chemically from that of a parent protein. Modified glycoform
typically refers to the different carbohydrate or oligosaccharide;
thus for example an Fc variant may comprise a modified glycoform.
Alternatively, modified glycoform may refer to the Fc variant that
comprises the different carbohydrate or oligosaccharide.
[0107] By "parent polypeptide", "parent protein", "parent
immunogloblin", "precursor polypeptide", "precursor protein", or
"precursor immunoglobulin" as used herein is meant an unmodified
polypeptide, protein, or immunoglobulin that is subsequently
modified to generate a variant, e.g., any polypeptide, protein or
immunoglobulin which serves as a template and/or basis for at least
one amino acid modification described herein. The parent
polypeptide may be a naturally occurring polypeptide, or a variant
or engineered version of a naturally occurring polypeptide. Parent
polypeptide may refer to the polypeptide itself, compositions that
comprise the parent polypeptide, or the amino acid sequence that
encodes it. Accordingly, by "parent Fc polypeptide" as used herein
is meant an Fc polypeptide that is modified to generate a variant
Fc polypeptide, and by "parent antibody" as used herein is meant an
antibody that is modified to generate a variant antibody (e.g., a
parent antibody may include, but is not limited to, a protein
comprising the constant region of a naturally occurring Ig).
[0108] By "position" as used herein is meant a location in the
sequence of a protein. Positions may be numbered sequentially, or
according to an established format, for example the EU index as in
Kabat. For example, position 297 is a position in the human
antibody IgG1.
[0109] By "polypeptide" or "protein" as used herein is meant at
least two covalently attached amino acids, which includes proteins,
polypeptides, oligopeptides and peptides.
[0110] By "residue" as used herein is meant a position in a protein
and its associated amino acid identity. For example, Asparagine 297
(also referred to as Asn297, also referred to as N297) is a residue
in the human antibody IgG1.
[0111] By "target antigen" as used herein is meant the molecule
that is bound by the variable region of a given antibody, or the
fusion partner of an Fc fusion. A target antigen may be a protein,
carbohydrate, lipid, or other chemical compound. An antibody or Fc
fusion is said to be "specific" for a given target antigen based on
having affinity for the target antigen.
[0112] By "target cell" as used herein is meant a cell that
expresses a target antigen.
[0113] By "variable region" as used herein is meant the region of
an immunoglobulin that comprises one or more Ig domains
substantially encoded by any of the V.kappa., V.lamda., and/or VH
genes that make up the kappa, lambda, and heavy chain
immunoglobulin genetic loci respectively.
[0114] By "variant polypeptide", "polypeptide variant", or
"variant" as used herein is meant a polypeptide sequence that
differs from that of a parent polypeptide sequence by virtue of at
least one amino acid modification. The parent polypeptide may be a
naturally occurring or wild-type (WT) polypeptide, or may be a
modified version of a WT polypeptide. Variant polypeptide may refer
to the polypeptide itself, a composition comprising the
polypeptide, or the amino sequence that encodes it. In some
embodiments, variant polypeptides disclosed herein (e.g., variant
immunoglobulins) may have at least one amino acid modification
compared to the parent polypeptide, e.g. from about one to about
ten amino acid modifications, from about one to about five amino
acid modifications, etc. compared to the parent. The variant
polypeptide sequence herein may possess at least about 80% homology
with a parent polypeptide sequence, e.g., at least about 90%
homology, 95% homology, etc. Accordingly, by "Fc variant" or
"variant Fc" as used herein is meant an Fc sequence that differs
from that of a parent Fc sequence by virtue of at least one amino
acid modification. An Fc variant may only encompass an Fc region,
or may exist in the context of an antibody, Fc fusion, isolated Fc,
Fc fragment, or other polypeptide that is substantially encoded by
Fc. Fc variant may refer to the Fc polypeptide itself, compositions
comprising the Fc variant polypeptide, or the amino acid sequence
that encodes it. By "Fc polypeptide variant" or "variant Fc
polypeptide" as used herein is meant an Fc polypeptide that differs
from a parent Fc polypeptide by virtue of at least one amino acid
modification. By "protein variant" or "variant protein" as used
herein is meant a protein that differs from a parent protein by
virtue of at least one amino acid modification. By "antibody
variant" or "variant antibody" as used herein is meant an antibody
that differs from a parent antibody by virtue of at least one amino
acid modification. By "IgG variant" or "variant IgG" as used herein
is meant an antibody that differs from a parent IgG by virtue of at
least one amino acid modification. By "immunoglobulin variant" or
"variant immunoglobulin" as used herein is meant an immunoglobulin
sequence that differs from that of a parent immunoglobulin sequence
by virtue of at least one amino acid modification.
[0115] By "wild type" or "WT" herein is meant an amino acid
sequence or a nucleotide sequence that is found in nature,
including allelic variations. A WT protein, polypeptide, antibody,
immunoglobulin, IgG, etc. has an amino acid sequence or a
nucleotide sequence that has not been intentionally modified.
[0116] Immunoqlobulins
[0117] As described herein, an immunoglobulin may be an antibody,
an Fc fusion, an isolated Fc, an Fc fragment, or an Fc polypeptide.
In one embodiment, an immunoglobulin is an antibody.
[0118] Antibodies are immunological proteins that bind a specific
antigen. In most mammals, including humans and mice, antibodies are
constructed from paired heavy and light polypeptide chains. The
light and heavy chain variable regions show significant sequence
diversity between antibodies, and are responsible for binding the
target antigen. Each chain is made up of individual immunoglobulin
(Ig) domains, and thus the generic term immunoglobulin is used for
such proteins.
[0119] Traditional antibody structural units typically comprise a
tetramer. Each tetramer is typically composed of two identical
pairs of polypeptide chains, each pair having one "light"
(typically having a molecular weight of about 25 kDa) and one
"heavy" chain (typically having a molecular weight of about 50-70
kDa). Human light chains are classified as kappa and lambda light
chains. Heavy chains are classified as mu, delta, gamma, alpha, or
epsilon, and define the antibody's isotype as IgM, IgD, IgG, IgA,
and IgE, respectively. IgG has several subclasses, including, but
not limited to IgG1, IgG2, IgG3, and IgG4. IgM has subclasses,
including, but not limited to, IgM1 and IgM2. IgA has several
subclasses, including but not limited to IgA1 and IgA2. Thus,
"isotype" as used herein is meant any of the classes and subclasses
of immunoglobulins defined by the chemical and antigenic
characteristics of their constant regions. The known human
immunoglobulin isotypes are IgG1, IgG2, IgG3, IgG4, IgA1, IgA2,
IgM1, IgM2, IgD, and IgE.
[0120] Each of the light and heavy chains are made up of two
distinct regions, referred to as the variable and constant regions.
The IgG heavy chain is composed of four immunoglobulin domains
linked from N- to C-terminus in the order VH-CH1-CH2-CH3, referring
to the heavy chain variable domain, heavy chain constant domain 1,
heavy chain constant domain 2, and heavy chain constant domain 3
respectively (also referred to as VH-C.gamma.1-C.gamma.2-C.gamma.3,
referring to the heavy chain variable domain, constant gamma 1
domain, constant gamma 2 domain, and constant gamma 3 domain
respectively). The IgG light chain is composed of two
immunoglobulin domains linked from N- to C-terminus in the order
VL-CL, referring to the light chain variable domain and the light
chain constant domain respectively. The constant regions show less
sequence diversity, and are responsible for binding a number of
natural proteins to elicit important biochemical events. The
distinguishing features between these antibody classes are their
constant regions, although subtler differences may exist in the
variable region.
[0121] The variable region of an antibody contains the antigen
binding determinants of the molecule, and thus determines the
specificity of an antibody for its target antigen. The variable
region is so named because it is the most distinct in sequence from
other antibodies within the same class. The amino-terminal portion
of each chain includes a variable region of about 100 to 110 or
more amino acids primarily responsible for antigen recognition. In
the variable region, three loops are gathered for each of the V
domains of the heavy chain and light chain to form an
antigen-binding site. Each of the loops is referred to as a
complementarity-determining region (hereinafter referred to as a
"CDR"), in which the variation in the amino acid sequence is most
significant. There are 6 CDRs total, three each per heavy and light
chain, designated VH CDR1, VH CDR2, VH CDR3, VL CDR1, VL CDR2, and
VL CDR3. The variable region outside of the CDRs is referred to as
the framework (FR) region. Although not as diverse as the CDRs,
sequence variability does occur in the FR region between different
antibodies. Overall, this characteristic architecture of antibodies
provides a stable scaffold (the FR region) upon which substantial
antigen binding diversity (the CDRs) can be explored by the immune
system to obtain specificity for a broad array of antigens. A
number of high-resolution structures are available for a variety of
variable region fragments from different organisms, some unbound
and some in complex with antigen. Sequence and structural features
of antibody variable regions are disclosed, for example, in Morea
et al., 1997, Biophys Chem 68:9-16; Morea et al., 2000, Methods
20:267-279, hereby entirely incorporated by reference, and the
conserved features of antibodies are disclosed, for example, in
Maynard et al., 2000, Annu Rev Biomed Eng 2:339-376, hereby
entirely incorporated by reference.
[0122] The carboxy-terminal portion of each chain defines a
constant region primarily responsible for effector function. In the
IgG subclass of immunoglobulins, there are several immunoglobulin
domains in the heavy chain. By "immunoglobulin (Ig) domain" herein
is meant a region of an immunoglobulin having a distinct tertiary
structure. Of interest in embodiments described herein are the
heavy chain domains, including, the constant heavy (CH) domains and
the hinge region. In the context of IgG antibodies, the IgG
isotypes each have three CH regions. Accordingly, "CH" domains in
the context of IgG are as follows: "CH1" refers to positions
118-220 according to the EU index as in Kabat. "CH2" refers to
positions 237-340 according to the EU index as in Kabat, and "CH3"
refers to positions 341-447 according to the EU index as in
Kabat.
[0123] Another important region of the heavy chain is the hinge
region. By "hinge" or "hinge region" or "antibody hinge region" or
"immunoglobulin hinge region" herein is meant the flexible
polypeptide comprising the amino acids between the first and second
constant domains of an antibody. Structurally, the IgG CH1 domain
ends at EU position 220, and the IgG CH2 domain begins at residue
EU position 237. Thus for IgG the antibody hinge is herein defined
to include positions 221 (D221 in IgG1) to 236 (G236 in IgG1),
wherein the numbering is according to the EU index as in Kabat. In
some embodiments, for example in the context of an Fc region, the
lower hinge is included, with the "lower hinge" generally referring
to positions 226 or 230 to 236.
[0124] Of interest in embodiments described herein are the Fc
regions. By "Fc" or "Fc region", as used herein is meant the
polypeptide comprising the constant region of an antibody excluding
the first constant region immunoglobulin domain and in some cases,
part of the hinge. Thus Fc refers to the last two constant region
immunoglobulin domains of IgA, IgD, and IgG, and the last three
constant region immunoglobulin domains of IgE and IgM, and the
flexible hinge N-terminal to these domains. For IgA and IgM, Fc may
include the J chain. For IgG, Fc comprises immunoglobulin domains
Cgamma2 and Cgamma3 (C.gamma.2 and C.gamma.3) and the lower hinge
region between Cgamma1 (C.gamma.1) and Cgamma2 (C.gamma.2).
Although the boundaries of the Fc region may vary, the human IgG
heavy chain Fc region is usually defined to include residues C226
or P230 to its carboxyl-terminus, wherein the numbering is
according to the EU index as in Kabat. Fc may refer to this region
in isolation, or this region in the context of an Fc polypeptide,
as described below. By "Fc polypeptide" as used herein is meant a
polypeptide that comprises all or part of an Fc region. Fc
polypeptides include antibodies, Fc fusions, isolated Fcs, and Fc
fragments.
[0125] The Fc region of an antibody interacts with a number of Fc
receptors and ligands, imparting an array of important functional
capabilities referred to as effector functions. For IgG the Fc
region, Fc comprises Ig domains C.gamma.2 and C.gamma.3 and the
N-terminal hinge leading into C.gamma.2. An important family of Fc
receptors for the IgG class are the Fc gamma receptors
(Fc.gamma.Rs). These receptors mediate communication between
antibodies and the cellular arm of the immune system (Raghavan et
al., 1996, Annu Rev Cell Dev Biol 12:181-220; Ravetch et al., 2001,
Annu Rev Immunol 19:275-290, both hereby entirely incorporated by
reference). In humans this protein family includes Fc.gamma.RI
(CD64), including isoforms Fc.gamma.RIa, Fc.gamma.RIb, and
Fc.gamma.RIc; Fc.gamma.RII (CD32), including isoforms Fc.gamma.RIIa
(including allotypes H131 and R131), Fc.gamma.RIIb (including
Fc.gamma.RIIb-1 and Fc.gamma.RIIb-2), and Fc.gamma.RIIc; and
Fc.gamma.RII (CD16), including isoforms Fc.gamma.RIIIa (including
allotypes V158 and F158) and Fc.gamma.RIIIb (including allotypes
Fc.gamma.RIIIb-NA1 and Fc.gamma.RIIIb-NA2) (Jefferis et al., 2002,
Immunol Lett 82:57-65, hereby entirely incorporated by reference).
These receptors typically have an extracellular domain that
mediates binding to Fc, a membrane spanning region, and an
intracellular domain that may mediate some signaling event within
the cell. These receptors are expressed in a variety of immune
cells including monocytes, macrophages, neutrophils, dendritic
cells, eosinophils, mast cells, platelets, B cells, large granular
lymphocytes, Langerhans' cells, natural killer (NK) cells, and
.gamma..gamma. T cells. Formation of the Fc/Fc.gamma.R complex
recruits these effector cells to sites of bound antigen, typically
resulting in signaling events within the cells and important
subsequent immune responses such as release of inflammation
mediators, B cell activation, endocytosis, phagocytosis, and
cytotoxic attack. The ability to mediate cytotoxic and phagocytic
effector functions is a potential mechanism by which antibodies
destroy targeted cells. The cell-mediated reaction wherein
nonspecific cytotoxic cells that express Fc.gamma.Rs recognize
bound antibody on a target cell and subsequently cause lysis of the
target cell is referred to as antibody dependent cell-mediated
cytotoxicity (ADCC) (Raghavan et al., 1996, Annu Rev Cell Dev Biol
12:181-220; Ghetie et al., 2000, Annu Rev Immunol 18:739-766;
Ravetch et al., 2001, Annu Rev Immunol 19:275-290, both hereby
entirely incorporated by reference). The cell-mediated reaction
wherein nonspecific cytotoxic cells that express Fc.gamma.Rs
recognize bound antibody on a target cell and subsequently cause
phagocytosis of the target cell is referred to as antibody
dependent cell-mediated phagocytosis (ADCP).
[0126] The different IgG subclasses have different affinities for
the Fc.gamma.Rs, with IgG1 and IgG3 typically binding substantially
better to the receptors than IgG2 and IgG4 (Jefferis et al., 2002,
Immunol Lett 82:57-65, hereby entirely incorporated by reference).
The Fc.gamma.Rs bind the IgG Fc region with different affinities.
The extracellular domains of Fc.gamma.RIIIa and Fc.gamma.RIIIb are
96% identical, however Fc.gamma.RIIIb does not have a intracellular
signaling domain. Furthermore, whereas Fc.gamma.RI,
Fc.gamma.RIIa/c, and Fc.gamma.RIIIa are positive regulators of
immune complex-triggered activation, characterized by having an
intracellular domain that has an immunoreceptor tyrosine-based
activation motif (ITAM), Fc.gamma.RIIb has an immunoreceptor
tyrosine-based inhibition motif (ITIM) and is therefore inhibitory.
Thus the former are referred to as activation receptors, and
Fc.gamma.RIIb is referred to as an inhibitory receptor. Despite
these differences in affinities and activities, all Fc.gamma.Rs
bind the same region on Fc, at the N-terminal end of the C.gamma.2
domain and the preceding hinge. This interaction is well
characterized structurally (Sondermann et al., 2001, J Mol Biol
309:737-749, hereby entirely incorporated by reference), and
several structures of the human Fc bound to the extracellular
domain of human Fc.gamma.RIIIb have been solved (pdb accession code
1 E4K) (Sondermann et al., 2000, Nature 406:267-273, hereby
entirely incorporated by reference) (pdb accession codes 1IIS and
1IIX) (Radaev et al., 2001, J Biol Chem 276:16469-16477, hereby
entirely incorporated by reference).
[0127] An overlapping but separate site on Fc serves as the
interface for the complement protein C1q. In the same way that
Fc/Fc.gamma.R binding mediates ADCC, Fc/C1q binding mediates
complement dependent cytotoxicity (CDC). A site on Fc between the
C.gamma.2 and C.gamma.3 domains mediates interaction with the
neonatal receptor FcRn, the binding of which recycles endocytosed
antibody from the endosome back to the bloodstream (Raghavan et
al., 1996, Annu Rev Cell Dev Biol 12:181-220; Ghetie et al., 2000,
Annu Rev Immunol 18:739-766, both hereby entirely incorporated by
reference). This process, coupled with preclusion of kidney
filtration due to the large size of the full length molecule,
results in favorable antibody serum half-lives ranging from one to
three weeks. Binding of Fc to FcRn also plays a key role in
antibody transport. The binding site for FcRn on Fc is also the
site at which the bacterial proteins A and G bind. The tight
binding by these proteins is typically exploited as a means to
purify antibodies by employing protein A or protein G affinity
chromatography during protein purification. The fidelity of these
regions, the complement and FcRn/protein A binding regions are
important for both the clinical properties of antibodies and their
development.
[0128] A key feature of the Fc region is the conserved N-linked
glycosylation that occurs at N297. This carbohydrate, or
oligosaccharide as it is sometimes referred, plays a critical
structural and functional role for the antibody, and is one of the
principle reasons that antibodies must be produced using mammalian
expression systems. Efficient Fc binding to Fc.gamma.R and C1q
requires this modification, and alterations in the composition of
the N297 carbohydrate or its elimination affect binding to these
proteins (Umana et al., 1999, Nat Biotechnol 17:176-180; Davies et
al., 2001, Biotechnol Bioeng 74:288-294; Mimura et al., 2001, J
Biol Chem 276:45539-45547.; Radaev et al., 2001, J Biol Chem
276:16478-16483; Shields et al., 2001, J Biol Chem 276:6591-6604;
Shields et al., 2002, J Biol Chem 277:26733-26740; Simmons et al.,
2002, J Immunol Methods 263:133-147, all hereby entirely
incorporated by reference).
[0129] Immunoglobulins of embodiments described herein may also be
an antibody-like protein referred to as an Fc fusion (Chamow et
al., 1996, Trends Biotechnol 14:52-60; Ashkenazi et al., 1997, Curr
Opin Immunol 9:195-200, both incorporated entirely by reference).
"Fc fusion" is herein meant to be synonymous with the terms
"immunoadhesin", "Ig fusion", "Ig chimera", and "receptor globulin"
(sometimes with dashes) as used in the prior art (Chamow et al.,
1996, Trends Biotechnol 14:52-60; Ashkenazi et al., 1997, Curr Opin
Immunol 9:195-200). An Fc fusion is a protein wherein one or more
polypeptides, herein referred to as a "fusion partner", is operably
linked to Fc. An Fc fusion combines the Fc region of an antibody,
and thus its favorable effector functions and pharmacokinetics,
with the target-binding region of a receptor, ligand, or some other
protein or protein domain. The role of the latter is to mediate
target recognition, and thus it is functionally analogous to the
antibody variable region. Because of the structural and functional
overlap of Fc fusions with antibodies, the discussion on antibodies
in the present disclosure extends also to Fc fusions.
[0130] Virtually any protein or small molecule may be linked to Fc
to generate an Fc fusion. Protein fusion partners may include, but
are not limited to, the variable region of any antibody, the
target-binding region of a receptor, an adhesion molecule, a
ligand, an enzyme, a cytokine, a chemokine, or some other protein
or protein domain. Small molecule fusion partners may include any
agent that directs the Fc fusion to a target antigen. Such target
antigen may be any molecule, e.g., an extracellular receptor, that
is implicated in disease. Fc fusions of embodiments described
herein may target virtually antigen that is expressed on CD32b+
cells.
[0131] Fusion partners may be linked to any region of an Fc region,
including at the N- or C-termini, or at some residue in-between the
termini. In one embodiment, a fusion partner is linked at the N- or
C-terminus of the Fc region. A variety of linkers may find use in
some embodiments described herein to covalently link Fc regions to
a fusion partner. By "linker", "linker sequence", "spacer",
"tethering sequence" or grammatical equivalents thereof, herein is
meant a molecule or group of molecules (such as a monomer or
polymer) that connects two molecules and often serves to place the
two molecules in a configuration. Linkers are known in the art; for
example, homo- or hetero-bifunctional linkers as are well known
(see, 1994 Pierce Chemical Company catalog, technical section on
cross-linkers, pages 155-200, incorporated entirely by reference).
A number of strategies may be used to covalently link molecules
together. These include, but are not limited to polypeptide
linkages between N- and C-termini of proteins or protein domains,
linkage via disulfide bonds, and linkage via chemical cross-linking
reagents. In one aspect of this embodiment, the linker is a peptide
bond, generated by recombinant techniques or peptide synthesis. The
linker peptide may predominantly include the following amino acid
residues: Gly, Ser, Ala, or Thr. The linker peptide should have a
length that is adequate to link two molecules in such a way that
they assume the correct conformation relative to one another so
that they retain the desired activity. Suitable lengths for this
purpose include at least one and not more than 50 amino acid
residues. In one embodiment, the linker is from about 1 to 30 amino
acids in length. In one embodiment, h linkers of 1 to 20 amino
acids in length may be used. Useful linkers include glycine-serine
polymers (including, for example, (GS)n, (GSGGS)n (set forth as SEQ
ID NO:1), (GGGGS)n (set forth as SEQ ID NO:2), and (GGGS)n (set
forth as SEQ ID NO:3), where n is an integer of at least one),
glycine-alanine polymers, alanine-serine polymers, and other
flexible linkers, as will be appreciated by those in the art.
Alternatively, a variety of nonproteinaceous polymers, including
but not limited to polyethylene glycol (PEG), polypropylene glycol,
polyoxyalkylenes, or copolymers of polyethylene glycol and
polypropylene glycol, may find use as linkers, that is may find use
to link an Fc regions to a fusion partner.
[0132] Also contemplated as fusion partners are Fc polypeptides.
Thus an immunoglobulin as described herein may be a multimeric Fc
polypeptide, comprising two or more Fc regions. The advantage of
such a molecule is that it provides multiple binding sites for Fc
receptors with a single protein molecule. In one embodiment, Fc
regions may be linked using a chemical engineering approach. For
example, Fab's and Fc's may be linked by thioether bonds
originating at cysteine residues in the hinges, generating
molecules such as FabFc.sub.2. Fc regions may be linked using
disulfide engineering and/or chemical cross-linking. In one
embodiment, Fc regions may be linked genetically. In one
embodiment, Fc regions in an immunoglobulin are linked genetically
to generated tandemly linked Fc regions as described in U.S. Ser.
No. 11/022,289, filed Dec. 21, 2004, entitled "Fc polypeptides with
novel Fc ligand binding sites," incorporated entirely by reference.
Tandemly linked Fc polypeptides may comprise two or more Fc
regions, e.g., one to three Fc regions, two Fc regions. It may be
advantageous to explore a number of engineering constructs in order
to obtain homo- or hetero-tandemly linked Fc regions with the most
favorable structural and functional properties. Tandemly linked Fc
regions may be homo-tandemly linked Fc regions, that is an Fc
region of one isotype is fused genetically to another Fc region of
the same isotype. It is anticipated that because there are multiple
Fc.gamma.R, C1q, and/or FcRn binding sites on tandemly linked Fc
polypeptides, effector functions and/or pharmacokinetics may be
enhanced. In an alternate embodiment, Fc regions from different
isotypes may be tandemly linked, referred to as hetero-tandemly
linked Fc regions. For example, because of the capacity to target
Fc.gamma.R and Fc.alpha.RI receptors, an immunoglobulin that binds
both Fc.gamma.Rs and Fc.alpha.RI may provide a significant clinical
improvement.
[0133] The immunoglobulins of embodiments disclosed herein may be
substantially encoded by immunoglobulin genes belonging to any of
the antibody classes. In certain embodiments, the immunoglobulins
disclosed herein find use in antibodies or Fc fusions that comprise
sequences belonging to the IgG class of antibodies, including IgG1,
IgG2, IgG3, or IgG4. FIG. 1 provides an alignment of these human
IgG sequences. In alternate embodiments, immunoglobulins disclosed
herein find use in antibodies or Fc fusions that comprise sequences
belonging to the IgA (including subclasses IgA1 and IgA2), IgD,
IgE, IgG, or IgM classes of antibodies. The immunoglobulins
disclosed herein may comprise more than one protein chain, e.g.,
may be an antibody or Fc fusion that is a monomer or an oligomer,
including a homo- or hetero-oligomer.
[0134] Immunoglobulins disclosed herein may be substantially
encoded by genes from any organism, e.g., mammals (including, but
not limited to humans, rodents (including but not limited to mice
and rats), lagomorpha (including but not limited to rabbits and
hares), camelidae (including but not limited to camels, llamas, and
dromedaries), and non-human primates, including but not limited to
Prosimians, Platyrrhini (New World monkeys), Cercopithecoidea (Old
World monkeys), and Hominoidea including the Gibbons and Lesser and
Great Apes. In a certain embodiments, the immunoglobulins disclosed
herein may be substantially human.
[0135] As is well known in the art, immunoglobulin polymorphisms
exist in the human population. Gm polymorphism is determined by the
IGHG1, IGHG2 and IGHG3 genes which have alleles encoding allotypic
antigenic determinants referred to as G1 m, G2m, and G3m allotypes
for markers of the human IgG1, IgG2 and IgG3 molecules (no Gm
allotypes have been found on the gamma 4 chain). Markers may be
classified into `allotypes` and `isoallotypes`. These are
distinguished on different serological bases dependent upon the
strong sequence homologies between isotypes. Allotypes are
antigenic determinants specified by allelic forms of the Ig genes.
Allotypes represent slight differences in the amino acid sequences
of heavy or light chains of different individuals. Even a single
amino acid difference can give rise to an allotypic determinant,
although in many cases there are several amino acid substitutions
that have occurred. Allotypes are sequence differences between
alleles of a subclass whereby the antisera recognize only the
allelic differences. An isoallotype is an allele in one isotype
which produces an epitope which is shared with a non-polymorphic
homologous region of one or more other isotypes and because of this
the antisera will react with both the relevant allotypes and the
relevant homologous isotypes (Clark, 1997, IgG effector mechanisms,
Chem Immunol. 65:88-110; Gorman & Clark, 1990, Semin Immunol
2(6):457-66, both hereby entirely incorporated by reference).
[0136] Allelic forms of human immunoglobulins have been
well-characterized (WHO Review of the notation for the allotypic
and related markers of human immunoglobulins. J Immunogen 1976, 3:
357-362; WHO Review of the notation for the allotypic and related
markers of human immunoglobulins. 1976, Eur. J. Immunol. 6,
599-601; Loghem E van, 1986, Allotypic markers, Monogr Allergy 19:
40-51, all hereby entirely incorporated by reference).
Additionally, other polymorphisms have been characterized (Kim et
al., 2001, J. Mol. Evol. 54:1-9, hereby entirely incorporated by
reference). At present, 18 Gm allotypes are known: G1 m (1, 2, 3,
17) or G1 m (a, x, f, z), G2m (23) or G2m (n), G3m (5, 6, 10, 11,
13, 14, 15, 16, 21, 24, 26, 27, 28) or G3m (b1, c3, b5, b0, b3, b4,
s, t, g1, c5, u, v, g5) (Lefranc, et al., The human IgG subclasses:
molecular analysis of structure, function and regulation. Pergamon,
Oxford, pp. 43-78 (1990); Lefranc, G. et al., 1979, Hum. Genet.:
50, 199-211, both hereby entirely incorporated by reference).
Allotypes that are inherited in fixed combinations are called Gm
haplotypes. FIG. 2 shows common haplotypes of the gamma chain of
human IgG1 (FIG. 2A) and IgG2 (FIG. 2A) showing the positions and
the relevant amino acid substitutions. The immunoglobulins
disclosed herein may be substantially encoded by any allotype,
isoallotype, or haplotype of any immunoglobulin gene.
[0137] The immunoglobulins disclosed herein may compose an Fc
polypeptide, including but not limited to antibodies, isolated Fcs,
Fc fragments, and Fc fusions. In one embodiment, an immunoglobulin
disclosed herein is a full length antibody, constituting the
natural biological form of an antibody, including variable and
constant regions. For the IgG isotype full length antibody is a
tetramer and consists of two identical pairs of two immunoglobulin
chains, each pair having one light and one heavy chain, each light
chain comprising immunoglobulin domains VL and CL, and each heavy
chain comprising immunoglobulin domains VH, C.gamma.1, C.gamma.2,
and C.gamma.3. In another embodiment, immunoglobulins disclosed
herein are isolated Fc regions or Fc fragments.
[0138] Immunoglobulins disclosed herein may be a variety of
structures, including, but not limited antibody fragments,
bispecific antibodies, minibodies, domain antibodies, synthetic
antibodies (sometimes referred to herein as "antibody mimetics"),
chimeric antibodies, humanized antibodies, antibody fusions
(sometimes referred to as "antibody conjugates"), and fragments of
each, respectively.
[0139] In one embodiment, the antibody is an antibody fragment.
Specific antibody fragments include, but are not limited to, (i)
the Fab fragment consisting of VL, VH, CL and CH1 domains, (ii) the
Fd fragment consisting of the VH and CH1 domains, (iii) the Fv
fragment consisting of the VL and VH domains of a single antibody;
(iv) the dAb fragment, which consists of a single variable, (v)
isolated CDR regions, (vi) F(ab')2 fragments, a bivalent fragment
comprising two linked Fab fragments (vii) single chain Fv molecules
(scFv), wherein a VH domain and a VL domain are linked by a peptide
linker which allows the two domains to associate to form an antigen
binding site, (viii) bispecific single chain Fv dimers, and (ix)
"diabodies" or "triabodies", multivalent or multispecific fragments
constructed by gene fusion. The antibody fragments may be modified.
For example, the molecules may be stabilized by the incorporation
of disulphide bridges linking the VH and VL domains. Examples of
antibody formats and architectures are described in Holliger &
Hudson, 2006, Nature Biotechnology 23(9):1126-1136, and Carter
2006, Nature Reviews Immunology 6:343-357 and references cited
therein, all expressly incorporated by reference.
[0140] In one embodiment, an antibody disclosed herein may be a
multispecific antibody, and notably a bispecific antibody, also
sometimes referred to as "diabodies". These are antibodies that
bind to two (or more) different antigens. Diabodies can be
manufactured in a variety of ways known in the art, e.g., prepared
chemically or from hybrid hybridomas. In one embodiment, the
antibody is a minibody. Minibodies are minimized antibody-like
proteins comprising a scFv joined to a CH3 domain. In some cases,
the scFv can be joined to the Fc region, and may include some or
all of the hinge region. For a description of multispecific
antibodies see Holliger & Hudson, 2006, Nature Biotechnology
23(9):1126-1136 and references cited therein, all expressly
incorporated by reference.
[0141] Nonhuman, Chimeric, Humanized, and Fully Human
Antibodies
[0142] The variable region of an antibody, as is well known in the
art, can compose sequences from a variety of species. In some
embodiments, the antibody variable region can be from a nonhuman
source, including but not limited to mice, rats, rabbits, camels,
llamas, and monkeys. In some embodiments, the scaffold components
can be a mixture from different species. As such, an antibody
disclosed herein may be a chimeric antibody and/or a humanized
antibody. In general, both "chimeric antibodies" and "humanized
antibodies" refer to antibodies that combine regions from more than
one species. For example, "chimeric antibodies" traditionally
comprise variable region(s) from a mouse or other nonhuman species
and the constant region(s) from a human.
[0143] "Humanized antibodies" generally refer to non-human
antibodies that have had the variable-domain framework regions
swapped for sequences found in human antibodies. Generally, in a
humanized antibody, the entire antibody, except the CDRs, is
encoded by a polynucleotide of human origin or is identical to such
an antibody except within its CDRs. The CDRs, some or all of which
are encoded by nucleic acids originating in a non-human organism,
are grafted into the beta-sheet framework of a human antibody
variable region to create an antibody, the specificity of which is
determined by the engrafted CDRs. The creation of such antibodies
is described in, e.g., WO 92/11018, Jones, 1986, Nature
321:522-525, Verhoeyen et al., 1988, Science 239:1534-1536.
"Backmutation" of selected acceptor framework residues to the
corresponding donor residues is often required to regain affinity
that is lost in the initial grafted construct (U.S. Pat. No.
5,693,762, incorporated entirely by reference. The humanized
antibody optimally also will comprise at least a portion of an
immunoglobulin constant region, typically that of a human
immunoglobulin, and thus will typically comprise a human Fc region.
Humanized antibodies can also be generated using mice with a
genetically engineered immune system. Roque et al., 2004,
Biotechnol. Prog. 20:639-654. A variety of techniques and methods
for humanizing and reshaping non-human antibodies are well known in
the art (See Tsurushita & Vasquez, 2004, Humanization of
Monoclonal Antibodies, Molecular Biology of B Cells, 533-545,
Elsevier Science (USA), and references cited therein). Humanization
or other methods of reducing the immunogenicity of nonhuman
antibody variable regions may include resurfacing methods, as
described for example in Roguska et al., 1994, Proc. Natl. Acad.
Sci. USA 91:969-973. In one embodiment, the parent antibody has
been affinity matured, as is known in the art. Structure-based
methods may be employed for humanization and affinity maturation,
for example as described in U.S. Ser. No. 11/004,590. Selection
based methods may be employed to humanize and/or affinity mature
antibody variable regions, that is, to increase the affinity of the
variable region for its target antigen. Other humanization methods
may involve the grafting of only parts of the CDRs, including but
not limited to methods described in U.S. Ser. No. 09/810,502; Tan
et al., 2002, J. Immunol. 169:1119-1125; De Pascalis et al., 2002,
J. Immunol. 169:3076-3084. Structure-based methods may be employed
for humanization and affinity maturation, for example as described
in U.S. Ser. No. 10/153,159 and related applications, all
incorporated entirely by reference. In certain variations, the
immunogenicity of the antibody is reduced using a method described
in U.S. Ser. No. 11/004,590, entitled "Methods of Generating
Variant Proteins with Increased Host String Content and
Compositions Thereof", filed on Dec. 3, 2004, incorporated entirely
by reference.
[0144] In one embodiment, the antibody is a fully human antibody
with at least one modification as outlined herein. "Fully human
antibody" or "complete human antibody" refers to a human antibody
having the gene sequence of an antibody derived from a human
chromosome with the modifications outlined herein. Fully human
antibodies may be obtained, for example, using transgenic mice
(Bruggemann et al., 1997, Curr Opin Biotechnol 8:455-458) or human
antibody libraries coupled with selection methods (Griffiths et
al., 1998, Curr Opin Biotechnol 9:102-108).
[0145] Target Antigens
[0146] Virtually any antigen may be targeted by the immunoglobulins
disclosed herein, including but not limited to proteins, subunits,
domains, motifs, and/or epitopes belonging to the following list of
targets: 17-IA, 4-1BB, 4Dc, 6-keto-PGF1 a, 8-iso-PGF2a, 8-oxo-dG,
A1 Adenosine Receptor, A33, ACE, ACE-2, Activin, Activin A, Activin
AB, Activin B, Activin C, Activin RIA, Activin RIA ALK-2, Activin
RIB ALK-4, Activin RIIA, Activin RIIB, ADAM, ADAM10, ADAM12,
ADAM15, ADAM17/TACE, ADAM8, ADAM9, ADAMTS, ADAMTS4, ADAMTS5,
Addressins, aFGF, ALCAM, ALK, ALK-1, ALK-7, alpha-1-antitrypsin,
alpha-V/beta-1 antagonist, ANG, Ang, APAF-1, APE, APJ, APP, APRIL,
AR, ARC, ART, Artemin, anti-Id, ASPARTIC, Atrial natriuretic
factor, av/b3 integrin, Axl, b2M, B7-1, B7-2, B7-H, B-lymphocyte
Stimulator (BlyS), BACE, BACE-1, Bad, BAFF, BAFF-R, Bag-1, BAK,
Bax, BCA-1, BCAM, Bcl, BCMA, BDNF, b-ECGF, bFGF, BID, Bik, BIM,
BLC, BL-CAM, BLK, BMP, BMP-2 BMP-2a, BMP-3 Osteogenin, BMP-4
BMP-2b, BMP-5, BMP-6 Vgr-1, BMP-7 (OP-1), BMP-8 (BMP-8a, OP-2),
BMPR, BMPR-IA (ALK-3), BMPR-IB (ALK-6), BRK-2, RPK-1, BMPR-II
(BRK-3), BMPs, b-NGF, BOK, Bombesin, Bone-derived neurotrophic
factor, BPDE, BPDE-DNA, BTC, complement factor 3 (C3), C3a, C4, C5,
C5a, C10, CA125, CAD-8, Calcitonin, cAMP, carcinoembryonic antigen
(CEA), carcinoma-associated antigen, Cathepsin A, Cathepsin B,
Cathepsin C/DPPI, Cathepsin D, Cathepsin E, Cathepsin H, Cathepsin
L, Cathepsin O, Cathepsin S, Cathepsin V, Cathepsin X/Z/P, CBL,
CCI, CCK2, CCL, CCL1, CCL11, CCL12, CCL13, CCL14, CCL15, CCL16,
CCL17, CCL18, CCL19, CCL2, CCL20, CCL21, CCL22, CCL23, CCL24,
CCL25, CCL26, CCL27, CCL28, CCL3, CCL4, CCL5, CCL6, CCL7, CCL8,
CCL9/10, CCR, CCR1, CCR10, CCR10, CCR2, CCR3, CCR4, CCR5, CCR6,
CCR7, CCR8, CCR9, CD1, CD2, CD3, CD3E, CD4, CD5, CD6, CD7, CD8,
CD10, CD11a, CD11b, CD11c, CD13, CD14, CD15, CD16, CD18, CD19,
CD20, CD21, CD22, CD23, CD25, CD27L, CD28, CD29, CD30, CD30L, CD32,
CD33 (p67 proteins), CD34, CD38, CD40, CD40L, CD44, CD45, CD46,
CD49a, CD52, CD54, CD55, CD56, CD61, CD64, CD66e, CD74, CD80
(B7-1), CD89, CD95, CD123, CD137, CD138, CD140a, CD146, CD147,
CD148, CD152, CD164, CEACAM5, CFTR, cGMP, CINC, Clostridium
botulinum toxin, Clostridium perfringens toxin, CKb8-1, CLC, CMV,
CMV UL, CNTF, CNTN-1, COX, C-Ret, CRG-2, CT-1, CTACK, CTGF, CTLA-4,
CX3CL1, CX3CR1, CXCL, CXCL1, CXCL2, CXCL3, CXCL4, CXCL5, CXCL6,
CXCL7, CXCL8, CXCL9, CXCL10, CXCL11, CXCL12, CXCL13, CXCL14,
CXCL15, CXCL16, CXCR, CXCR1, CXCR2, CXCR3, CXCR4, CXCR5, CXCR6,
cytokeratin tumor-associated antigen, DAN, DCC, DcR3, DC-SIGN,
Decay accelerating factor, des(1-3)-IGF-I (brain IGF-1), Dhh,
digoxin, DNAM-1, Dnase, Dpp, DPPIV/CD26, Dtk, ECAD, EDA, EDA-A1,
EDA-A2, EDAR, EGF, EGFR (ErbB-1), EMA, EMMPRIN, ENA, endothelin
receptor, Enkephalinase, eNOS, Eot, eotaxinl, EpCAM, Ephrin
B2/EphB4, EPO, ERCC, E-selectin, ET-1, Factor IIa, Factor VII,
Factor VIIIc, Factor IX, fibroblast activation protein (FAP), Fas,
FcR1, FEN-1, Ferritin, FGF, FGF-19, FGF-2, FGF3, FGF-8, FGFR,
FGFR-3, Fibrin, FL, FLIP, Flt-3, Flt-4, Follicle stimulating
hormone, Fractalkine, FZD1, FZD2, FZD3, FZD4, FZD5, FZD6, FZD7,
FZD8, FZD9, FZD10, G250, Gas 6, GCP-2, GCSF, GD2, GD3, GDF, GDF-1,
GDF-3 (Vgr-2), GDF-5 (BMP-14, CDMP-1), GDF-6 (BMP-13, CDMP-2),
GDF-7 (BMP-12, CDMP-3), GDF-8 (Myostatin), GDF-9, GDF-15 (MIC-1),
GDNF, GDNF, GFAP, GFRa-1, GFR-alphal, GFR-alpha2, GFR-alpha3, GITR,
Glucagon, Glut 4, glycoprotein IIb/IIIa (GP IIb/IIIa), GM-CSF,
gp130, gp72, GRO, Growth hormone releasing factor, Hapten (NP-cap
or NIP-cap), HB-EGF, HCC, HCMV gB envelope glycoprotein, HCMV) gH
envelope glycoprotein, HCMV UL, Hemopoietic growth factor (HGF),
Hep B gp120, heparanase, Her2, Her2/neu (ErbB-2), Her3 (ErbB-3),
Her4 (ErbB-4), herpes simplex virus (HSV) gB glycoprotein, HSV gD
glycoprotein, HGFA, High molecular weight melanoma-associated
antigen (HMW-MAA), HIV gp120, HIV IIIB gp 120 V3 loop, HLA, HLA-DR,
HLA-DP, HLA-DQ, CD317/HM1.24, HMFG PEM, HRG, Hrk, human cardiac
myosin, human cytomegalovirus (HCMV), human growth hormone (HGH),
HVEM, 1-309, IAP, ICAM, ICAM-1, ICAM-3, ICE, ICOS, IFNg, Ig, IgA
receptor, IgE, IGF, IGF binding proteins, IGF-1R, IGFBP, IGF-I,
IGF-II, IL, IL-1, IL-1R, IL-2, IL-2R, IL-4, IL-4R, IL-5, IL-5R,
IL-6, IL-6R, IL-8, IL-9, IL-10, IL-12, IL-13, IL-15, IL-18, IL-18R,
IL-23, interferon (INF)-alpha, INF-beta, INF-gamma, Inhibin, iNOS,
Insulin A-chain, Insulin B-chain, Insulin-like growth factor 1,
integrin alpha2, integrin alpha3, integrin alpha4, integrin
alpha4/beta1, integrin alpha4/beta7, integrin alpha5 (alphaV),
integrin alpha5/beta1, integrin alpha5/beta3, integrin alpha6,
integrin beta1, integrin beta2, interferon gamma, IP-10, I-TAC, JE,
Kallikrein 2, Kallikrein 5, Kallikrein 6, Kallikrein 11, Kallikrein
12, Kallikrein 14, Kallikrein 15, Kallikrein L1, Kallikrein L2,
Kallikrein L3, Kallikrein L4, KC, KDR, Keratinocyte Growth Factor
(KGF), laminin 5, LAMP, LAP, LAP (TGF-1), Latent TGF-1, Latent
TGF-1 bp1, LBP, LDGF, LECT2, Lefty, Lewis-Y antigen, Lewis-Y
related antigen, LFA-1, LFA-3, Lfo, LIF, LIGHT, lipoproteins, LIX,
LKN, Lptn, L-Selectin, LT-a, LT-b, LTB4, LTBP-1, Lung surfactant,
Luteinizing hormone, Lymphotoxin Beta Receptor, Mac-1, MAdCAM, MAG,
MAP2, MARC, MCAM, MCAM, MCK-2, MCP, M-CSF, MDC, Mer,
METALLOPROTEASES, MGDF receptor, MGMT, MHC (HLA-DR), MIF, MIG, MIP,
MIP-1-alpha, MK, MMAC1, MMP, MMP-1, MMP-10, MMP-11, MMP-12, MMP-13,
MMP-14, MMP-15, MMP-2, MMP-24, MMP-3, MMP-7, MMP-8, MMP-9, MPIF,
Mpo, MSK, MSP, mucin (Mucl), MUC18, Muellerian-inhibitin substance,
Mug, MuSK, NAIP, NAP, NCAD, N-Cadherin, NCA 90, NCAM, NCAM,
Neprilysin, Neurotrophin-3, -4, or -6, Neurturin, Neuronal growth
factor (NGF), NGFR, NGF-beta, nNOS, NO, NOS, Npn, NRG-3, NT, NTN,
OB, OGG1, OPG, OPN, OSM, OX40L, OX40R, p150, p95, PADPr,
Parathyroid hormone, PARC, PARP, PBR, PBSF, PCAD, P-Cadherin, PCNA,
PDGF, PDGF, PDK-1, PECAM, PEM, PF4, PGE, PGF, PGI2, PGJ2, PIN,
PLA2, placental alkaline phosphatase (PLAP), PIGF, PLP, PP14,
Proinsulin, Prorelaxin, Protein C, PS, PSA, PSCA, prostate specific
membrane antigen (PSMA), PTEN, PTHrp, Ptk, PTN, R51, RANK, RANKL,
RANTES, RANTES, Relaxin A-chain, Relaxin B-chain, renin,
respiratory syncytial virus (RSV) F, RSV Fgp, Ret, Rheumatoid
factors, RLIP76, RPA2, RSK, S100, SCF/KL, SDF-1, SERINE, Serum
albumin, sFRP-3, Shh, SIGIRR, SK-1, SLAM, SLPI, SMAC, SMDF, SMOH,
SOD, SPARC, Stat, STEAP, STEAP-II, TACE, TACI, TAG-72
(tumor-associated glycoprotein-72), TARC, TCA-3, T-cell receptors
(e.g., T-cell receptor alpha/beta), TdT, TECK, TEM1, TEM5, TEM7,
TEM8, TERT, testicular PLAP-like alkaline phosphatase, TfR, TGF,
TGF-alpha, TGF-beta, TGF-beta Pan Specific, TGF-beta RI (ALK-5),
TGF-beta RII, TGF-beta RIIb, TGF-beta RIII, TGF-beta1, TGF-beta2,
TGF-beta3, TGF-beta4, TGF-beta5, Thrombin, Thymus Ck-1, Thyroid
stimulating hormone, Tie, TIMP, TIQ, Tissue Factor, TMEFF2, Tmpo,
TMPRSS2, TNF, TNF-alpha, TNF-alpha beta, TNF-beta2, TNFc, TNF-RI,
TNF-RII, TNFRSF10A (TRAIL R1 Apo-2, DR4), TNFRSF10B (TRAIL R2 DR5,
KILLER, TRICK-2A, TRICK-B), TNFRSF10C (TRAIL R3 DcR1, LIT, TRID),
TNFRSF10D (TRAIL R4 DcR2, TRUNDD), TNFRSF11A (RANK ODF R, TRANCE
R), TNFRSF11B (OPG OCIF, TR1), TNFRSF12 (TWEAK R FN14), TNFRSF13B
(TACI), TNFRSF13C (BAFF R), TNFRSF14 (HVEM ATAR, HveA, LIGHT R,
TR2), TNFRSF16 (NGFR p75NTR), TNFRSF17 (BCMA), TNFRSF18 (GITR
AITR), TNFRSF19 (TROY TAJ, TRADE), TNFRSF19L (RELT), TNFRSF1A (TNF
RI CD120a, p55-60), TNFRSF1B (TNF RII CD120b, p75-80), TNFRSF26
(TNFRH3), TNFRSF3 (LTbR TNF RIII, TNFC R), TNFRSF4 (OX40 ACT35,
TXGP1R), TNFRSF5 (CD40 p50), TNFRSF6 (Fas Apo-1, APT1, CD95),
TNFRSF6B (DcR3 M68, TR6), TNFRSF7 (CD27), TNFRSF8 (CD30), TNFRSF9
(4-1BB CD137, ILA), TNFRSF21 (DR6), TNFRSF22 (DcTRAIL R2 TNFRH2),
TNFRST23 (DcTRAIL R1 TNFRH1), TNFRSF25 (DR3 Apo-3, LARD, TR-3,
TRAMP, WSL-1), TNFSF10 (TRAIL Apo-2 Ligand, TL2), TNFSF11
(TRANCE/RANK Ligand ODF, OPG Ligand), TNFSF12 (TWEAK Apo-3 Ligand,
DR3 Ligand), TNFSF13 (APRIL TALL2), TNFSF13B (BAFF BLYS, TALL1,
THANK, TNFSF20), TNFSF14 (LIGHT HVEM Ligand, LTg), TNFSF15
(TL1A/VEGI), TNFSF18 (GITR Ligand AITR Ligand, TL6), TNFSF1A (TNF-a
Conectin, DIF, TNFSF2), TNFSF1B (TNF-b LTa, TNFSF1), TNFSF3 (LTb
TNFC, p33), TNFSF4 (OX40 Ligand gp34, TXGP1), TNFSF5 (CD40 Ligand
CD154, gp39, HIGM1, IMD3, TRAP), TNFSF6 (Fas Ligand Apo-1 Ligand,
APT1 Ligand), TNFSF7 (CD27 Ligand CD70), TNFSF8 (CD30 Ligand
CD153), TNFSF9 (4-1BB Ligand CD137 Ligand), TP-1, t-PA, Tpo, TRAIL,
TRAIL R, TRAIL-R1, TRAIL-R2, TRANCE, transferring receptor, TRF,
Trk, TROP-2, TSG, TSLP, tumor-associated antigen CA 125,
tumor-associated antigen expressing Lewis Y related carbohydrate,
TWEAK, TXB2, Ung, uPAR, uPAR-1, Urokinase, VCAM, VCAM-1, VECAD,
VE-Cadherin, VE-cadherin-2, VEFGR-1 (flt-1), VEGF, VEGFR, VEGFR-3
(flt-4), VEGI, VIM, Viral antigens, VLA, VLA-1, VLA-4, VNR
integrin, von Willebrands factor, WIF-1, WNT1, WNT2, WNT2B/13,
WNT3, WNT3A, WNT4, WNT5A, WNT5B, WNT6, WNT7A, WNT7B, WNT8A, WNT8B,
WNT9A, WNT9A, WNT9B, WNT10A, WNT10B, WNT11, WNT16, XCL1, XCL2,
XCR1, XCR1, XEDAR, XIAP, XPD, and receptors for hormones and growth
factors, etc.
[0147] In one embodiment, antigens are those that are expressed on
CD32b+ cells, e.g., B cell proteins, e.g., one or more proteins of
the B cell receptor complex. Target antigens include, but are not
limited to, CD19, CD20, CD21 (CR2), CD22, CD23/Fc.epsilon.RII,
Fc.epsilon.RI, (.alpha., .beta., and .gamma. subunits),
CD24/BBA-1/HSA, CD27, CD35 (CR1), CD38, CD40, CD45RA,
CD52/CAMPATH-1/HE5, CD72, CD79a (Ig.alpha.), CD79b (Ig(.beta.), IgM
(.mu.), CD80, CD81, CD86, Leu13, HLA-DR, -DP, -DQ, CD138,
CD317/HM1.24, CD11a, CD11b, CD11c, CD14, CD68, CD163, CD172a,
CD200R, and CD206. In one embodiment, the immunoglobulins disclosed
herein are also specific for a target antigen selected from the
group consisting of: IgM (.mu.), CD19, CD20, CD21, CD22, CD23,
CD24, CD35, CD40, CD45RA, CD72, CD79a, CD79b, CD80, CD81, CD86, and
HLA-DR. In one embodiment, immunoglobulins disclosed herein are
also specific for a target antigen selected from the group
consisting of: IgM (.mu.), CD79a, CD79b, CD19, CD21, CD22, CD72,
CD81, and Leu13. In one embodiment, immunoglobulins disclosed
herein are also specific for a target antigen selected from the
group consisting of: p, CD19, CD79a, Cd79b, CD81, and HLA-DR. In
another embodiment, immunoglobulins disclosed herein are also
specific for a target antigen selected from the group consisting
of: CD22, CD40, and CD72.
[0148] In another embodiment, target antigens may include those
that are bound, or may be bound, to the surface of B cells. For
example, immunoglobulins disclosed herein may also target
autoimmune antigens (i.e., autoantigens) or allergens. In one
embodiment, autoimmune antigens that may be targeted by the
immunoglobulins disclosed herein include but are not limited to
double-stranded DNA, platelet antigens, myelin protein antigen, Sm
antigens in snRNPs, islet cell antigen, Rheumatoid factor, and
anticitrullinated protein. citrullinated proteins and peptides such
as CCP-1, CCP-2 (cyclical citrullinated peptides), fibrinogen,
fibrin, vimentin, fillaggrin, collagen I and II peptides,
alpha-enolase, translation initiation factor 4G1, perinuclear
factor, keratin, Sa (cytoskeletal protein vimentin), components of
articular cartilage such as collagen II, IX, and XI, circulating
serum proteins such as RFs (IgG, IgM), fibrinogen, plasminogen,
ferritin, nuclear components such as RA33/hnRNP A2, Sm, eukaryotic
trasnlation elogation factor 1 alpha 1, stress proteins such as
HSP-65, -70, -90, BiP, inflammatory/immune factors such as B7-H1,
IL-1 alpha, and IL-8, enzymes such as calpastatin, alpha-enolase,
aldolase-A, dipeptidyl peptidase, osteopontin, glucose-6-phosphate
isomerase, receptors such as lipocortin 1, neutrophil nuclear
proteins such as lactoferrin and 25-35 kD nuclear protein, granular
proteins such as bactericidal permeability increasing protein
(BPI), elastase, cathepsin G, myeloperoxidase, proteinase 3,
platelet antigens, myelin protein antigen, islet cell antigen,
rheumatoid factor, histones, ribosomal P proteins, cardiolipin,
vimentin, nucleic acids such as dsDNA, ssDNA, and RNA, ribonuclear
particles and proteins such as Sm antigens (including but not
limited to SmD's and SmB'/B), U1RNP, A2/B1 hnRNP, Ro (SSA), and La
(SSB) antigens.
[0149] Fc Variants and Fc Receptor Binding Properties
[0150] Immunoglobulins disclosed herein may comprise an Fc variant.
An Fc variant comprises one or more amino acid modifications
relative to a parent Fc polypeptide, wherein the amino acid
modification(s) provide one or more optimized properties. An Fc
variant disclosed herein differs in amino acid sequence from its
parent by virtue of at least one amino acid modification. Thus Fc
variants disclosed herein have at least one amino acid modification
compared to the parent. Alternatively, the Fc variants disclosed
herein may have more than one amino acid modification as compared
to the parent, for example from about one to fifty amino acid
modifications, e.g., from about one to ten amino acid
modifications, from about one to about five amino acid
modifications, etc. compared to the parent. Thus the sequences of
the Fc variants and those of the parent Fc polypeptide are
substantially homologous. For example, the variant Fc variant
sequences herein will possess about 80% homology with the parent Fc
variant sequence, e.g., at least about 90% homology, at least about
95% homology, at least about 98% homology, at least about 99%
homology, etc. Modifications disclosed herein include amino acid
modifications, including insertions, deletions, and substitutions.
Modifications disclosed herein also include glycoform
modifications. Modifications may be made genetically using
molecular biology, or may be made enzymatically or chemically.
[0151] Fc variants disclosed herein are defined according to the
amino acid modifications that compose them. Thus, for example,
S267E is an Fc variant with the substitution S267E relative to the
parent Fc polypeptide. Likewise, S267E/L328F defines an Fc variant
with the substitutions S267E and L328F relative to the parent Fc
polypeptide. The identity of the WT amino acid may be unspecified,
in which case the aforementioned variant is referred to as
267E/328F. It is noted that the order in which substitutions are
provided is arbitrary, that is to say that, for example, 267E/328F
is the same Fc variant as 328F/267E, and so on. Unless otherwise
noted, positions discussed herein are numbered according to the EU
index or EU numbering scheme (Kabat et al., 1991, Sequences of
Proteins of Immunological Interest, 5th Ed., United States Public
Health Service, National Institutes of Health, Bethesda, hereby
entirely incorporated by reference). The EU index or EU index as in
Kabat or EU numbering scheme refers to the numbering of the EU
antibody (Edelman et al., 1969, Proc Natl Acad Sci USA 63:78-85,
hereby entirely incorporated by reference).
[0152] In certain embodiments, the Fc variants disclosed herein are
based on human IgG sequences, and thus human IgG sequences are used
as the "base" sequences against which other sequences are compared,
including but not limited to sequences from other organisms, for
example rodent and primate sequences. Immunoglobulins may also
comprise sequences from other immunoglobulin classes such as IgA,
IgE, IgGD, IgGM, and the like. It is contemplated that, although
the Fc variants disclosed herein are engineered in the context of
one parent IgG, the variants may be engineered in or "transferred"
to the context of another, second parent IgG. This is done by
determining the "equivalent" or "corresponding" residues and
substitutions between the first and second IgG, typically based on
sequence or structural homology between the sequences of the first
and second IgGs. In order to establish homology, the amino acid
sequence of a first IgG outlined herein is directly compared to the
sequence of a second IgG. After aligning the sequences, using one
or more of the homology alignment programs known in the art (for
example using conserved residues as between species), allowing for
necessary insertions and deletions in order to maintain alignment
(i.e., avoiding the elimination of conserved residues through
arbitrary deletion and insertion), the residues equivalent to
particular amino acids in the primary sequence of the first
immunoglobulin are defined. Alignment of conserved residues may
conserve 100% of such residues. However, alignment of greater than
75% or as little as 50% of conserved residues is also adequate to
define equivalent residues. Equivalent residues may also be defined
by determining structural homology between a first and second IgG
that is at the level of tertiary structure for IgGs whose
structures have been determined. In this case, equivalent residues
are defined as those for which the atomic coordinates of two or
more of the main chain atoms of a particular amino acid residue of
the parent or precursor (N on N, CA on CA, C on C and O on O) are
within about 0.13 nm, after alignment. In another embodiment,
equivalent residues are within about 0.1 nm after alignment.
Alignment is achieved after the best model has been oriented and
positioned to give the maximum overlap of atomic coordinates of
non-hydrogen protein atoms of the proteins. Regardless of how
equivalent or corresponding residues are determined, and regardless
of the identity of the parent IgG in which the IgGs are made, what
is meant to be conveyed is that the Fc variants discovered as
disclosed herein may be engineered into any second parent IgG that
has significant sequence or structural homology with the Fc
variant. Thus for example, if a variant antibody is generated
wherein the parent antibody is human IgG1, by using the methods
described above or other methods for determining equivalent
residues, the variant antibody may be engineered in another IgG1
parent antibody that binds a different antigen, a human IgG2 parent
antibody, a human IgA parent antibody, a mouse IgG2a or IgG2b
parent antibody, and the like. Again, as described above, the
context of the parent Fc variant does not affect the ability to
transfer the Fc variants disclosed herein to other parent IgGs.
[0153] The Fc variants disclosed herein may be optimized for a
variety of Fc receptor binding properties. An Fc variant that is
engineered or predicted to display one or more optimized properties
is herein referred to as an "optimized Fc variant". Properties that
may be optimized include but are not limited to enhanced or reduced
affinity for an Fc.gamma.R. In one embodiment, the Fc variants
disclosed herein are optimized to possess enhanced affinity for an
inhibitory receptor Fc.gamma.RIIb. In other embodiments,
immunoglobulins disclosed herein provide enhanced affinity for
Fc.gamma.RIIb, yet reduced affinity for one or more activating
Fc.gamma.Rs, including for example Fc.gamma.RI, Fc.gamma.RIIa,
Fc.gamma.RIIIa, and/or Fc.gamma.RIIIb. The Fc.gamma.R receptors may
be expressed on cells from any organism, including but not limited
to human, cynomolgus monkeys, and mice. The Fc variants disclosed
herein may be optimized to possess enhanced affinity for human
Fc.gamma.RIIb.
[0154] By "greater affinity" or "improved affinity" or "enhanced
affinity" or "better affinity" than a parent Fc polypeptide, as
used herein is meant that an Fc variant binds to an Fc receptor
with a significantly higher equilibrium constant of association
(K.sub.A or Ka) or lower equilibrium constant of dissociation
(K.sub.D or Kd) than the parent Fc polypeptide when the amounts of
variant and parent polypeptide in the binding assay are essentially
the same. For example, the Fc variant with improved Fc receptor
binding affinity may display from about 5 fold to about 1000 fold,
e.g. from about 10 fold to about 500 fold improvement in Fc
receptor binding affinity compared to the parent Fc polypeptide,
where Fc receptor binding affinity is determined, for example, by
the binding methods disclosed herein, including but not limited to
Biacore, by one skilled in the art.
[0155] Accordingly, by "reduced affinity" as compared to a parent
Fc polypeptide as used herein is meant that an Fc variant binds an
Fc receptor with significantly lower K.sub.A or higher K.sub.D than
the parent Fc polypeptide. Greater or reduced affinity can also be
defined relative to an absolute level of affinity. For example,
according to the data herein, WT (native) IgG1 binds Fc.gamma.RIIb
with an affinity of about 1.5 .mu.M, or about 1500 nM. Furthermore,
some Fc variants described herein bind Fc.gamma.RIIb with an
affinity about 10-fold greater to WT IgG1. As disclosed herein,
greater or enhanced affinity means having a K.sub.D lower than
about 100 nM, for example between about 10 nM-about 100 nM, between
about 1-about 100 nM, or less than about 1 nM.
[0156] In one embodiment, the Fc variants provide selectively
enhanced affinity to Fc.gamma.RIIb relative to one or more
activating receptors. Selectively enhanced affinity means either
that the Fc variant has improved affinity for Fc.gamma.RIIb
relative to the activating receptor(s) as compared to the parent Fc
polypeptide but has reduced affinity for the activating receptor(s)
as compared to the parent Fc polypeptide, or it means that the Fc
variant has improved affinity for both Fc.gamma.RIIb and activating
receptor(s) as compared to the parent Fc polypeptide, however the
improvement in affinity is greater for Fc.gamma.RIIb than it is for
the activating receptor(s). In alternate embodiments, the Fc
variants reduce or ablate binding to one or more activating
Fc.gamma.Rs, reduce or ablate binding to one or more complement
proteins, reduce or ablate one or more Fc.gamma.R-mediated effector
functions, and/or reduce or ablate one or more complement-mediated
effector functions.
[0157] The presence of different polymorphic forms of Fc.gamma.Rs
provides yet another parameter that impacts the therapeutic utility
of the Fc variants disclosed herein. Whereas the specificity and
selectivity of a given Fc variant for the different classes of
Fc.gamma.Rs significantly affects the capacity of an Fc variant to
target a given antigen for treatment of a given disease, the
specificity or selectivity of an Fc variant for different
polymorphic forms of these receptors may in part determine which
research or pre-clinical experiments may be appropriate for
testing, and ultimately which patient populations may or may not
respond to treatment. Thus the specificity or selectivity of Fc
variants disclosed herein to Fc receptor polymorphisms, including
but not limited to Fc.gamma.RIIa, Fc.gamma.RIIIa, and the like, may
be used to guide the selection of valid research and pre-clinical
experiments, clinical trial design, patient selection, dosing
dependence, and/or other aspects concerning clinical trials.
[0158] Fc variants disclosed herein may comprise modifications that
modulate interaction with Fc receptors other than Fc.gamma.Rs,
including but not limited to complement proteins, FcRn, and Fc
receptor homologs (FcRHs). FcRHs include but are not limited to
FcRH1, FcRH2, FcRH3, FcRH4, FcRH5, and FcRH6 (Davis et al., 2002,
Immunol. Reviews 190:123-136).
[0159] An important parameter that determines the most beneficial
selectivity of a given Fc variant to treat a given disease is the
context of the Fc variant. Thus the Fc receptor selectivity or
specificity of a given Fc variant will provide different properties
depending on whether it composes an antibody, Fc fusion, or Fc
variants with a coupled fusion partner. In one embodiment, an Fc
receptor specificity of the Fc variant disclosed herein will
determine its therapeutic utility. The utility of a given Fc
variant for therapeutic purposes will depend on the epitope or form
of the target antigen and the disease or indication being treated.
For some targets and indications, greater Fc.gamma.RIIb affinity
and reduced activating Fc.gamma.R-mediated effector functions may
be beneficial. For other target antigens and therapeutic
applications, it may be beneficial to increase affinity for
Fc.gamma.RIIb, or increase affinity for both Fc.gamma.RIIb and
activating receptors.
[0160] Inhibitory Properties and Methods of Inhibiting CD32b+
Cells
[0161] Target antigens of immunoglobulins disclosed herein may be
expressed on a variety of cell types. In some embodiments,
immunoglobulins disclosed herein are specific for antigens
expressed on CD32b+ cells. Cell types that may be targeted by the
immunoglobulins disclosed herein include, but are not limited to, B
cells, plasma cells, dendritic cells, macrophages, neutrophils,
mast cells, basophils, and eosinophils. In alternative embodiments,
the immunoglobulins disclosed herein may inhibit CD32b+ cells by
targeting an antigen not expressed on CD32b+ cells. In some
embodiments, target antigens include those that are not expressed
by CD32b+ cells, but may be bound to CD32b+ cells, e.g., via the
BCR. For example, in certain embodiments, the immunoglobulins may
target an autoimmune antigen or allergen. Autoimmune antigens that
may be targeted by the immunoglobulins disclosed herein include but
are not limited to citrullinated proteins and peptides such as
CCP-1, CCP-2 (cyclical citrullinated peptides), fibrinogen, fibrin,
vimentin, fillaggrin, collagen I and II peptides, alpha-enolase,
translation initiation factor 4G1, perinuclear factor, keratin, Sa
(cytoskeletal protein vimentin), components of articular cartilage
such as collagen II, IX, and XI, circulating serum proteins such as
RFs (IgG, IgM), fibrinogen, plasminogen, ferritin, nuclear
components such as RA33/hnRNP A2, Sm, eukaryotic trasnlation
elogation factor 1 alpha 1, stress proteins such as HSP-65, -70,
-90, BiP, inflammatory/immune factors such as B7-H1, IL-1 alpha,
and IL-8, enzymes such as calpastatin, alpha-enolase, aldolase-A,
dipeptidyl peptidase, osteopontin, glucose-6-phosphate isomerase,
receptors such as lipocortin 1, neutrophil nuclear proteins such as
lactoferrin and 25-35 kD nuclear protein, granular proteins such as
bactericidal permeability increasing protein (BPI), elastase,
cathepsin G, myeloperoxidase, proteinase 3, platelet antigens,
myelin protein antigen, islet cell antigen, rheumatoid factor,
histones, ribosomal P proteins, cardiolipin, vimentin, nucleic
acids such as dsDNA, ssDNA, and RNA, ribonuclear particles and
proteins such as Sm antigens (including but not limited to SmD's
and SmB'/B), U1RNP, A2/B1 hnRNP, Ro (SSA), and La (SSB)
antigens.
[0162] Disclosed herein are methods of inhibiting CD32b+ cells.
Without being limited thereto, FIG. 3 is a schematic representation
of a proposed mechanism by which immunoglobulins disclosed herein
inhibit CD32b+ cells (See also Example 3; see also FIG. 3).
Accordingly, disclosed herein are methods of inhibiting CD32b+
cells comprising contacting a CD32b+ cell with an immunoglobulin
comprising an Fc region with enhanced affinity to Fc.gamma.RIIb. In
one embodiment, the immunoglobulin binds at least two B cell
proteins, .e.g., at least to proteins bound to the surface B cells.
In one embodiment, the first of said B cell proteins is
Fc.gamma.RIIb. In a another embodiment, the second of said B cell
proteins is part of the B cell receptor (BCR) complex, which may
include an antigen bound to BCR. In another embodiment, the second
of said B cell proteins is not involved directly in antigen
recognition. In another embodiment, said the second of said B cell
proteins is expressed on the surface of the B cell, but is not part
of the B cell receptor. Nonlimiting examples of the second of said
B cell proteins include BCR proteins (e.g., IgM (.mu.), CD79a,
CD79b, CD19, CD21, CD22, CD72, CD81, Leu13, etc.), antigens bound
to the BCR (e.g., autoantigens, allergens, etc.), or other proteins
bound to the surface of B cells (e.g., CD20, CD23, CD24, CD35,
CD40, CD45RA, CD80, CD86, HLA-DR, etc.). In some embodiments, the
immunoglobulins inhibit release of calcium from the B cells upon
their stimulation through the B cell receptor. In another
embodiment, an immunoglobulin disclosed herein binds at least two B
cell proteins on the surface of the same B cell (see, .e.g., FIG.
3).
[0163] Modifications for Optimizing Inhibitory Function
[0164] Disclosed herein is directed to immunoglobulins comprising
modifications, wherein said modifications alter affinity to the
Fc.gamma.RIIb receptor, and/or alter the ability of the
immunoglobulin to mediate one or more Fc.gamma.RIIb-mediated
effector functions. Modifications of the invention include amino
acid modifications and glycoform modifications.
[0165] Amino Acid Modifications
[0166] As described herein (see, e.g., Example 9), simultaneous
high affinity coengagement of cognate BCR and Fc.gamma.RIIb may be
used to inhibit Fc.gamma.RIIb+ cells. Such coengagment may occur
via the use of an immunoglobulin described herein, e.g., an
immunoglobulin used to coengage both Fc.gamma.RIIb via its Fc
region, and a target antigen on the surface of the Fc.gamma.RIIb+
cell (e.g., one or more cognate BCR proteins and/or an antigen
bound to cognate BCR) via its Fv region. Amino acid modifications
at heavy chain constant region positions: 234, 235, 236, 237, 239,
265, 266, 267, 268, 298, 325, 326, 327, 328, 329, 330, 331 and 332
allow modification of immunoglobulin Fc.gamma.RIIb binding
properties, effector function, and potentially clinical properties
of antibodies
[0167] In one embodiment, immunoglobulins that bind Fc.gamma.RIIb+
cells and coengage a target antigen on the cell's surface and an
Fc.gamma.RIIb on cell's surface disclosed herein may be variant
immunoglobulins relative to a parent immunoglobulin. In one
embodiment, the variant immunoglobulin comprises a variant Fc
region, wherein said variant Fc region comprises one or more (e.g.,
two or more) modification(s) compared to a parent Fc region,
wherein said modification(s) are at positions selected from the
group consisting of 234, 235, 236, 237, 239, 265, 266, 267, 268,
298, 325, 326, 327, 328, 329, 330, 331, and 332, wherein numbering
is according to the EU index. In one embodiment, the variant
immunoglobulin comprises a variant Fc region, wherein said variant
Fc region comprises one or more (e.g., two or more) modification(s)
compared to a parent Fc region, wherein said modification(s) are at
positions selected from the group consisting of 234, 235, 236, 237,
239, 266, 267, 268, 325, 326, 327, 328, and 332, according to the
EU index. In one embodiment, the variant immunoglobulin comprises a
variant Fc region, wherein said variant Fc region comprises one or
more (e.g., two or more) modification(s) compared to a parent Fc
region, wherein said modification(s) are at positions selected from
the group consisting of 235, 236, 239, 266, 267, 268, and 328,
according to the EU index.
[0168] In one embodiment, said modification(s) is at least one
substitution (e.g., one or more substitution(s), two or more
substitution(s), etc.) selected from the group consisting of 234F,
234G, 234I, 234K, 234N, 234P, 234Q, 234S, 234V, 234W, 234Y, 234D,
234E, 235A, 235E, 235H, 235I, 235N, 235P, 235Q, 235R, 235S, 235W,
235Y, 235D, 235F, 235T, 236D, 236F, 236H, 236I, 236K, 236L, 236M,
236P, 236Q, 236R, 236S, 236T, 236V, 236W, 236Y, 236A, 236E, 236N,
237A, 237E, 237H, 237K, 237L, 237P, 237Q, 237S, 237V, 237Y, 237D,
237N, 239D, 239E, 239N, 239Q, 265E, 266D, 266I, 266M, 267A, 267D,
267E, 267G, 268D, 268E, 268N, 268Q, 298D, 298E, 298L, 298M, 298Q,
325L, 326A, 326E, 326W, 326D, 327D, 327G, 327L, 327N, 327Q, 327E,
328E, 328F, 328Y, 328H, 328I, 328Q, 328W, 329E, 330D, 330H, 330K,
330S, 331S, and 332E, wherein numbering is according to an EU
index. In one embodiment, said modification(s) is at least one
substitution (e.g., one or more substitution(s), two or more
substitution(s), etc.) selected from the group consisting of 234N,
234F, 234D, 234E, 234W, 235Q, 235R, 235W, 235Y, 235D, 235F, 235T,
236D, 236H, 236I, 236L, 236S, 236Y, 236E, 236N, 237H, 237L, 237D,
237N, 239D, 239N, 239E, 266I, 266M, 267A, 267D, 267E, 267G, 268D,
268E, 268N, 268Q, 298E, 298L, 298M, 298Q, 325L, 326A, 326E, 326W,
326D, 327D, 327L, 327E, 328E, 328F, 328Y, 328H, 328I, 328Q, 328W,
330D, 330H, 330K, and 332E, wherein numbering is according to an EU
index. In one embodiment, said modification(s) is at least one
substitution (e.g., one or more substitution(s), two or more
substitution(s), etc.) selected from the group consisting of 234D,
234E, 234W, 235D, 235F, 235R, 235Y, 236D, 236N, 237D, 237N, 239D,
239E, 266M, 267D, 267E, 268D, 268E, 327D, 327E, 328F, 328W, 328Y,
and 332E, wherein numbering is according to an EU index. In one
embodiment, said modification(s) is at least one substitution
(e.g., one or more substitution(s), two or more substitution(s),
etc.) selected from the group consisting of 235Y, 236D, 239D, 266M,
267E, 268D, 268E, 328F, 328W, and 328Y, wherein numbering is
according to an EU index.
[0169] In one embodiment, said modification(s) is at least two
modifications (e.g., a combination of modifications) at positions
selected from the group consisting of 234/239, 234/267, 234/328,
235/236, 235/239, 235/267, 235/268, 235/328, 236/239, 236/267,
236/268, 236/328, 237/267, 239/267, 239/268, 239/327, 239/328,
239/332, 266/267, 267/268, 267/325, 267/327, 267/328, 267/332,
268/327, 268/328, 268/332, 326/328, 327/328, and 328/332, wherein
numbering is according to an EU index. In one embodiment, said
modification(s) is at least two modifications (e.g., a combination
of modifications) at positions selected from the group consisting
of 235/267, 236/267, 239/268, 239/267, 267/268, and 267/328,
wherein numbering is according to an EU index. In one embodiment,
said modification(s) is at least two substitutions (e.g., a
combination of substitutions) selected from the group consisting of
234D/267E, 234E/267E, 234F/267E, 234E/328F, 234W/239D, 234W/239E,
234W/267E, 234W/328Y, 235D/267E, 235D/328F, 235F/239D, 235F/267E,
235F/328Y, 235Y/236D, 235Y/239D, 235Y/267D, 235Y/267E, 235Y/268E,
235Y/328F, 236D/239D, 236D/267E, 236D/268E, 236D/328F, 236N/267E,
237D/267E, 237N/267E, 239D/267D, 239D/267E, 239D/268D, 239D/268E,
239D/327D, 239D/328F, 239D/328W, 239D/328Y, 239D/332E, 239E/267E,
266M/267E, 267D/268E, 267E/268D, 267E/268E, 267E/325L, 267E/327D,
267E/327E, 267E/328F, 267E/328I, 267E/328Y, 267E/332E, 268D/327D,
268D/328F, 268D/328W, 268D/328Y, 268D/332E, 268E/328F, 268E/328Y,
327D/328Y, 328F/332E, 328W/332E, and 328Y/332E, wherein numbering
is according to an EU index.
[0170] In one embodiment, said modification(s) result in at least
one of the following substitutions, or combinations of
substitutions: 234F/236N, 234F/236D, 236A/237A, 236S/237A,
235D/239D, 234D/267E, 234E/267E, 234F/267E, 235D/267E, 235F/267E,
235S/267E, 235T/267E, 235Y/267D, 235Y/267E, 236D/267E, 236E/267E,
236N/267E, 237D/267E, 237N/267E, 239D/267D, 239D/267E, 266/267E,
234E/268D, 236D/268D, 239D/268D, 267D/268D, 267D/268E, 267E/268D,
267E/268E, 267E/325L, 267D/327D, 267D/327E, 267E/327D, 267E/327E,
268D/327D, 239D/328Y, 267E/328F, 267E/328H, 267E/328I, 267E/328Q,
267E/328Y, 268D/328Y, 239D/332E, 328Y/332E, 234D/236N/267E,
235Y/236D/267E, 234W/239E/267E, 235Y/239D/267E, 236D/239D/267E,
235Y/267E/268E, 236D/267E/268E, 239D/267E/268E, 234W/239D/328Y,
235F/239D/328Y, 234E/267E/328F, 235D/267E/328F, 235Y/267E/328F,
236D/267E/328F, 239D/267A/328Y, 239D/267E/328F, 234W/268D/328Y,
235F/268D/328Y, 239D/268D/328F, 239D/268D/328W, 239D/268D/328Y,
239D/268E/328Y, 267A/268D/328Y, 267E/268E/328F, 239D/326D/328Y,
268D/326D/328Y, 239D/327D/328Y, 268D/327D/328Y, 239D/267E/332E,
234W/328Y/332E, 235F/328Y/332E, 239D/328F/332E, 239D/328Y/332E,
267A/328Y/332E, 268D/328F/332E, 268D/328W/332E, 268D/328Y/332E,
268E/328Y/332E, 326D/328Y/332E, 327D/328Y/332E,
234W/236D/239E/267E, 239D/268D/328F/332E, 239D/268D/328W/332E, and
239D/268D/328Y/332E, wherein numbering is according to an EU index.
In one embodiment, said modification(s) result in at least one of
the following substitutions, or combinations of substitutions:
266D, 234F/236N, 234F/236D, 236A/237A, 236S/237A, 235D/239D,
234D/267E, 234E/267E, 234F/267E, 235D/267E, 235F/267E, 235S/267E,
235T/267E, 235Y/267D, 236D/267E, 236E/267E, 236N/267E, 237D/267E,
237N/267E, 266M/267E, 234E/268D, 236D/268D, 267D/268D, 267D/268E,
267E/268D, 267E/268E, 267E/325L, 267D/327D, 267D/327E, 267E/327E,
268D/327D, 239D/328Y, 267E/328F, 267E/328H, 267E/328I, 267E/328Q,
267E/328Y, 268D/328Y, 234D/236N/267E, 235Y/236D/267E,
234W/239E/267E, 235Y/239D/267E, 236D/239D/267E, 235Y/267E/268E,
236D/267E/268E, 234W/239D/328Y, 235F/239D/328Y, 234E/267E/328F,
235D/267E/328F, 235Y/267E/328F, 236D/267E/328F, 239D/267A/328Y,
239D/267E/328F, 234W/268D/328Y, 235F/268D/328Y, 239D/268D/328F,
239D/268D/328W, 239D/268D/328Y, 239D/268E/328Y, 267A/268D/328Y,
267E/268E/328F, 239D/326D/328Y, 268D/326D/328Y, 239D/327D/328Y,
268D/327D/328Y, 234W/328Y/332E, 235F/328Y/332E, 239D/328F/332E,
239D/328Y/332E, 267A/328Y/332E, 268D/328F/332E, 268D/328W/332E,
268D/328Y/332E, 268E/328Y/332E, 326D/328Y/332E, 327D/328Y/332E,
234W/236D/239E/267E, 239D/268D/328F/332E, 239D/268D/328W/332E, and
239D/268D/328Y/332E, wherein numbering is according to an EU index.
In one embodiment, said modification(s) result in at least one of
the following substitutions, or combinations of substitutions:
234N, 235Q, 235R, 235W, 235Y, 236D, 236H, 236I, 236L, 236S, 236Y,
237H, 237L, 239D, 239N, 266I, 266M, 267A, 267D, 267E, 267G, 268D,
268E, 268N, 268Q, 298E, 298L, 298M, 298Q, 326A, 326E, 326W, 327D,
327L, 328E, 328F, 330D, 330H, 330K, 234F/236N, 234F/236D,
235D/239D, 234D/267E, 234E/267E, 234F/267E, 235D/267E, 235F/267E,
235T/267E, 235Y/267D, 235Y/267E, 236D/267E, 236E/267E, 236N/267E,
237D/267E, 237N/267E, 239D/267D, 239D/267E, 266M/267E, 234E/268D,
236D/268D, 239D/268D, 267D/268D, 267D/268E, 267E/268D, 267E/268E,
267E/325L, 267D/327D, 267D/327E, 267E/327D, 267E/327E, 268D/327D,
239D/328Y, 267E/328F, 267E/328H, 267E/328I, 267E/328Q, 267E/328Y,
268D/328Y, 239D/332E, 328Y/332E, 234D/236N/267E, 235Y/236D/267E,
234W/239E/267E, 235Y/239D/267E, 236D/239D/267E, 235Y/267E/268E,
236D/267E/268E, 239D/267E/268E, 234W/239D/328Y, 235F/239D/328Y,
234E/267E/328F, 235D/267E/328F, 235Y/267E/328F, 236D/267E/328F,
239D/267A/328Y, 239D/267E/328F, 234W/268D/328Y, 235F/268D/328Y,
239D/268D/328F, 239D/268D/328W, 239D/268D/328Y, 239D/268E/328Y,
267A/268D/328Y, 267E/268E/328F, 239D/326D/328Y, 268D/326D/328Y,
239D/327D/328Y, 268D/327D/328Y, 239D/267E/332E, 234W/328Y/332E,
235F/328Y/332E, 239D/328F/332E, 239D/328Y/332E, 267A/328Y/332E,
268D/328F/332E, 268D/328W/332E, 268D/328Y/332E, 268E/328Y/332E,
326D/328Y/332E, 327D/328Y/332E, 234W/236D/239E/267E,
239D/268D/328F/332E, 239D/268D/328W/332E, and
239D/268D/328Y/332E
[0171] In one embodiment, said modification(s) result in at least
one of the following substitutions, or combinations of
substitutions: 235Y/267E, 236D/267E, 239D/268D, 239D/267E,
267E/268D, 267E/268E, and 267E/328F, wherein numbering is according
to an EU index.
[0172] In some embodiments, antibodies may comprise isotypic
modifications, that is, modifications in a parent IgG to the amino
acid type in an alternate IgG. For example as illustrated in FIG.
1, an IgG1/IgG3 hybrid variant may be constructed by substituting
IgG1 positions in the CH2 and/or CH3 region with the amino acids
from IgG3 at positions where the two isotypes differ. Thus a hybrid
variant IgG antibody may be constructed that comprises one or more
substitutions selected from the group consisting of: 274Q, 276K,
300F, 339T, 356E, 358M, 384S, 392N, 397M, 4221, 435R, and 436F. In
other embodiments of the invention, an IgG1/IgG2 hybrid variant may
be constructed by substituting IgG2 positions in the CH2 and/or CH3
region with amino acids from IgG1 at positions where the two
isotypes differ. Thus a hybrid variant IgG antibody may be
constructed that comprises one or more modifications selected from
the group consisting of 233E, 234L, 235L, -236G (referring to an
insertion of a glycine at position 236), and 327A.
[0173] Means for Optimizing Effector Function
[0174] Described herein are immunoglobulins comprising means for
alter affinity to the Fc.gamma.RIIb receptor, and/or alter the
ability of the immunoglobulin to mediate one or more
Fc.gamma.RIIb-mediated effector functions. Means of the invention
include amino acid modifications (e.g., positional means for
optimizing effector function, substitutional means for optimizing
effector function, etc.) and glycoform modifications (e.g., means
for glycoform modifications).
[0175] Amino Acid Modifications
[0176] As described herein, positional means for optimizing
effector function include but is not limited to, modification of an
amino acid at one or more heavy chain constant region positions
(e.g., at positions: 234, 235, 236, 237, 239, 265, 266, 267, 268,
298, 325, 326, 327, 328, 329, 330, 331, and 332) which allow
modification of immunoglobulin Fc.gamma.RIIb binding properties,
effector function, and potentially clinical properties of
antibodies.
[0177] In particular, substitutional means for optimizing
Fc.gamma.RIIb effector functions, e.g., by altering affinity to
Fc.gamma.RIIb, include, but is not limited to, a substitution of an
amino acid at one or more heavy chain constant region positions,
e.g., one or more of the amino acid substitutions in the following
heavy chain constant region positions: 234, 235, 236, 237, 239,
265, 266, 267, 268, 298, 325, 326, 327, 328, 329, 330, 331, and
332, wherein numbering is according to the EU index. In one
embodiment, substitutional means include at least one (e.g., two or
more) substitution(s) compared to a parent Fc region, wherein said
modification(s) are at positions selected from the group consisting
of 234, 235, 236, 237, 239, 266, 267, 268, 325, 326, 327, 328, and
332, according to the EU index. In one embodiment, substitional
means include one or more (e.g., two or more) substitions(s) at
positions selected from the group consisting of 235, 236, 239, 266,
267, 268, and 328, according to the EU index.
[0178] In one embodiment, said substitional means is at least one
substitution (e.g., one or more substitution(s), two or more
substitution(s), etc.) selected from the group consisting of 234F,
234G, 234I, 234K, 234N, 234P, 234Q, 234S, 234V, 234W, 234Y, 234D,
234E, 235A, 235E, 235H, 235I, 235N, 235P, 235Q, 235R, 235S, 235W,
235Y, 235D, 235F, 235T, 236D, 236F, 236H, 236I, 236K, 236L, 236M,
236P, 236Q, 236R, 236S, 236T, 236V, 236W, 236Y, 236A, 236E, 236N,
237A, 237E, 237H, 237K, 237L, 237P, 237Q, 237S, 237V, 237Y, 237D,
237N, 239D, 239E, 239N, 239Q, 265E, 266D, 266I, 266M, 267A, 267D,
267E, 267G, 268D, 268E, 268N, 268Q, 298D, 298E, 298L, 298M, 298Q,
325L, 326A, 326E, 326W, 326D, 327D, 327G, 327L, 327N, 327Q, 327E,
328E, 328F, 328Y, 328H, 328I, 328Q, 328W, 329E, 330D, 330H, 330K,
330S, 331S, and 332E, wherein numbering is according to an EU
index. In one embodiment, said substitional means is at least one
substitution (e.g., one or more substitution(s), two or more
substitution(s), etc.) selected from the group consisting of 234N,
234F, 234D, 234E, 234W, 235Q, 235R, 235W, 235Y, 235D, 235F, 235T,
236D, 236H, 236I, 236L, 236S, 236Y, 236E, 236N, 237H, 237L, 237D,
237N, 239D, 239N, 239E, 266I, 266M, 267A, 267D, 267E, 267G, 268D,
268E, 268N, 268Q, 298E, 298L, 298M, 298Q, 325L, 326A, 326E, 326W,
326D, 327D, 327L, 327E, 328E, 328F, 328Y, 328H, 328I, 328Q, 328W,
330D, 330H, 330K, and 332E, wherein numbering is according to an EU
index. In one embodiment, said substitional means is at least one
substitution (e.g., one or more substitution(s), two or more
substitution(s), etc.) selected from the group consisting of 234D,
234E, 234W, 235D, 235F, 235R, 235Y, 236D, 236N, 237D, 237N, 239D,
239E, 266M, 267D, 267E, 268D, 268E, 327D, 327E, 328F, 328W, 328Y,
and 332E, wherein numbering is according to an EU index. In one
embodiment, said substitional means is at least one substitution
(e.g., one or more substitution(s), two or more substitution(s),
etc.) selected from the group consisting of 235Y, 236D, 239D, 266M,
267E, 268D, 268E, 328F, 328W, and 328Y, wherein numbering is
according to an EU index.
[0179] In one embodiment, said substitional means is at least two
substitutions (e.g., a combination of modifications) at positions
selected from the group consisting of 234/239, 234/267, 234/328,
235/236, 235/239, 235/267, 235/268, 235/328, 236/239, 236/267,
236/268, 236/328, 237/267, 239/267, 239/268, 239/327, 239/328,
239/332, 266/267, 267/268, 267/325, 267/327, 267/328, 267/332,
268/327, 268/328, 268/332, 326/328, 327/328, and 328/332, wherein
numbering is according to an EU index. In one embodiment, said
substitional means is at least two substitutions (e.g., a
combination of modifications) at positions selected from the group
consisting of 235/267, 236/267, 239/268, 239/267, 267/268, and
267/328, wherein numbering is according to an EU index. In one
embodiment, said substitional means is at least two substitutions
(e.g., a combination of substitutions) selected from the group
consisting of 234D/267E, 234E/267E, 234F/267E, 234E/328F,
234W/239D, 234W/239E, 234W/267E, 234W/328Y, 235D/267E, 235D/328F,
235F/239D, 235F/267E, 235F/328Y, 235Y/236D, 235Y/239D, 235Y/267D,
235Y/267E, 235Y/268E, 235Y/328F, 236D/239D, 236D/267E, 236D/268E,
236D/328F, 236N/267E, 237D/267E, 237N/267E, 239D/267D, 239D/267E,
239D/268D, 239D/268E, 239D/327D, 239D/328F, 239D/328W, 239D/328Y,
239D/332E, 239E/267E, 266M/267E, 267D/268E, 267E/268D, 267E/268E,
267E/325L, 267E/327D, 267E/327E, 267E/328F, 267E/328I, 267E/328Y,
267E/332E, 268D/327D, 268D/328F, 268D/328W, 268D/328Y, 268D/332E,
268E/328F, 268E/328Y, 327D/328Y, 328F/332E, 328W/332E, and
328Y/332E, wherein numbering is according to an EU index.
[0180] In one embodiment, said substitional means result in at
least one of the following substitutions, or combinations of
substitutions: 234F/236N, 234F/236D, 236A/237A, 236S/237A,
235D/239D, 234D/267E, 234E/267E, 234F/267E, 235D/267E, 235F/267E,
235S/267E, 235T/267E, 235Y/267D, 235Y/267E, 236D/267E, 236E/267E,
236N/267E, 237D/267E, 237N/267E, 239D/267D, 239D/267E, 266/267E,
234E/268D, 236D/268D, 239D/268D, 267D/268D, 267D/268E, 267E/268D,
267E/268E, 267E/325L, 267D/327D, 267D/327E, 267E/327D, 267E/327E,
268D/327D, 239D/328Y, 267E/328F, 267E/328H, 267E/328I, 267E/328Q,
267E/328Y, 268D/328Y, 239D/332E, 328Y/332E, 234D/236N/267E,
235Y/236D/267E, 234W/239E/267E, 235Y/239D/267E, 236D/239D/267E,
235Y/267E/268E, 236D/267E/268E, 239D/267E/268E, 234W/239D/328Y,
235F/239D/328Y, 234E/267E/328F, 235D/267E/328F, 235Y/267E/328F,
236D/267E/328F, 239D/267A/328Y, 239D/267E/328F, 234W/268D/328Y,
235F/268D/328Y, 239D/268D/328F, 239D/268D/328W, 239D/268D/328Y,
239D/268E/328Y, 267A/268D/328Y, 267E/268E/328F, 239D/326D/328Y,
268D/326D/328Y, 239D/327D/328Y, 268D/327D/328Y, 239D/267E/332E,
234W/328Y/332E, 235F/328Y/332E, 239D/328F/332E, 239D/328Y/332E,
267A/328Y/332E, 268D/328F/332E, 268D/328W/332E, 268D/328Y/332E,
268E/328Y/332E, 326D/328Y/332E, 327D/328Y/332E,
234W/236D/239E/267E, 239D/268D/328F/332E, 239D/268D/328W/332E, and
239D/268D/328Y/332E, wherein numbering is according to an EU index.
In one embodiment, said substitional means result in at least one
of the following substitutions, or combinations of substitutions:
266D, 234F/236N, 234F/236D, 236A/237A, 236S/237A, 235D/239D,
234D/267E, 234E/267E, 234F/267E, 235D/267E, 235F/267E, 235S/267E,
235T/267E, 235Y/267D, 236D/267E, 236E/267E, 236N/267E, 237D/267E,
237N/267E, 266M/267E, 234E/268D, 236D/268D, 267D/268D, 267D/268E,
267E/268D, 267E/268E, 267E/325L, 267D/327D, 267D/327E, 267E/327E,
268D/327D, 239D/328Y, 267E/328F, 267E/328H, 267E/328I, 267E/328Q,
267E/328Y, 268D/328Y, 234D/236N/267E, 235Y/236D/267E,
234W/239E/267E, 235Y/239D/267E, 236D/239D/267E, 235Y/267E/268E,
236D/267E/268E, 234W/239D/328Y, 235F/239D/328Y, 234E/267E/328F,
235D/267E/328F, 235Y/267E/328F, 236D/267E/328F, 239D/267A/328Y,
239D/267E/328F, 234W/268D/328Y, 235F/268D/328Y, 239D/268D/328F,
239D/268D/328W, 239D/268D/328Y, 239D/268E/328Y, 267A/268D/328Y,
267E/268E/328F, 239D/326D/328Y, 268D/326D/328Y, 239D/327D/328Y,
268D/327D/328Y, 234W/328Y/332E, 235F/328Y/332E, 239D/328F/332E,
239D/328Y/332E, 267A/328Y/332E, 268D/328F/332E, 268D/328W/332E,
268D/328Y/332E, 268E/328Y/332E, 326D/328Y/332E, 327D/328Y/332E,
234W/236D/239E/267E, 239D/268D/328F/332E, 239D/268D/328W/332E, and
239D/268D/328Y/332E, wherein numbering is according to an EU index.
In one embodiment, said substitional means result in at least one
of the following substitutions, or combinations of substitutions:
234N, 235Q, 235R, 235W, 235Y, 236D, 236H, 236I, 236L, 236S, 236Y,
237H, 237L, 239D, 239N, 266I, 266M, 267A, 267D, 267E, 267G, 268D,
268E, 268N, 268Q, 298E, 298L, 298M, 298Q, 326A, 326E, 326W, 327D,
327L, 328E, 328F, 330D, 330H, 330K, 234F/236N, 234F/236D,
235D/239D, 234D/267E, 234E/267E, 234F/267E, 235D/267E, 235F/267E,
235T/267E, 235Y/267D, 235Y/267E, 236D/267E, 236E/267E, 236N/267E,
237D/267E, 237N/267E, 239D/267D, 239D/267E, 266M/267E, 234E/268D,
236D/268D, 239D/268D, 267D/268D, 267D/268E, 267E/268D, 267E/268E,
267E/325L, 267D/327D, 267D/327E, 267E/327D, 267E/327E, 268D/327D,
239D/328Y, 267E/328F, 267E/328H, 267E/328I, 267E/328Q, 267E/328Y,
268D/328Y, 239D/332E, 328Y/332E, 234D/236N/267E, 235Y/236D/267E,
234W/239E/267E, 235Y/239D/267E, 236D/239D/267E, 235Y/267E/268E,
236D/267E/268E, 239D/267E/268E, 234W/239D/328Y, 235F/239D/328Y,
234E/267E/328F, 235D/267E/328F, 235Y/267E/328F, 236D/267E/328F,
239D/267A/328Y, 239D/267E/328F, 234W/268D/328Y, 235F/268D/328Y,
239D/268D/328F, 239D/268D/328W, 239D/268D/328Y, 239D/268E/328Y,
267A/268D/328Y, 267E/268E/328F, 239D/326D/328Y, 268D/326D/328Y,
239D/327D/328Y, 268D/327D/328Y, 239D/267E/332E, 234W/328Y/332E,
235F/328Y/332E, 239D/328F/332E, 239D/328Y/332E, 267A/328Y/332E,
268D/328F/332E, 268D/328W/332E, 268D/328Y/332E, 268E/328Y/332E,
326D/328Y/332E, 327D/328Y/332E, 234W/236D/239E/267E,
239D/268D/328F/332E, 239D/268D/328W/332E, and
239D/268D/328Y/332E
[0181] In one embodiment, said substitional means result in at
least one of the following substitutions, or combinations of
substitutions: 235Y/267E, 236D/267E, 239D/268D, 239D/267E,
267E/268D, 267E/268E, and 267E/328F, wherein numbering is according
to an EU index.
[0182] In some embodiments of the invention, immunoglobulin may
comprise means for isotypic modifications, that is, modifications
in a parent IgG to the amino acid type in an alternate IgG. For
example as illustrated in FIG. 2A, an IgG1/IgG3 hybrid variant may
be constructed by a substitutional means for substituting IgG1
positions in the CH2 and/or CH3 region with the amino acids from
IgG3 at positions where the two isotypes differ. Thus a hybrid
variant IgG antibody may be constructed that comprises one or more
substitutional means, e.g., 274Q, 276K, 300F, 339T, 356E, 358M,
384S, 392N, 397M, 4221, 435R, and 436F. In other embodiments of the
invention, an IgG1/IgG2 hybrid variant may be constructed by a
substitutional means for substituting IgG2 positions in the CH2
and/or CH3 region with amino acids from IgG1 at positions where the
two isotypes differ. Thus a hybrid variant IgG antibody may be
constructed that comprises one or more substitutional means, e.g.,
one or more of the following amino acid substations: 233E, 234L,
235L, -236G (referring to an insertion of a glycine at position
236), and 327A.
[0183] Glycoform Modifications
[0184] Many polypeptides, including antibodies, are subjected to a
variety of post-translational modifications involving carbohydrate
moieties, such as glycosylation with oligosaccharides. There are
several factors that can influence glycosylation. The species,
tissue and cell type have all been shown to be important in the way
that glycosylation occurs. In addition, the extracellular
environment, through altered culture conditions such as serum
concentration, may have a direct effect on glycosylation (Lifely et
al., 1995, Glycobiology 5(8): 813-822).
[0185] All antibodies contain carbohydrate at conserved positions
in the constant regions of the heavy chain. Each antibody isotype
has a distinct variety of N-linked carbohydrate structures. Aside
from the carbohydrate attached to the heavy chain, up to 30% of
human IgGs have a glycosylated Fab region. IgG has a single
N-linked biantennary carbohydrate at Asn297 of the CH2 domain. For
IgG from either serum or produced ex vivo in hybridomas or
engineered cells, the IgG are heterogeneous with respect to the
Asn297 linked carbohydrate (Jefferis et al., 1998, Immunol. Rev.
163:59-76; Wright et al., 1997, Trends Biotech 15:26-32). For human
IgG, the core oligosaccharide normally consists of
GlcNAc.sub.2Man.sub.3GlcNAc, with differing numbers of outer
residues.
[0186] The carbohydrate moieties of immunoglobulins disclosed
herein will be described with reference to commonly used
nomenclature for the description of oligosaccharides. A review of
carbohydrate chemistry which uses this nomenclature is found in
Hubbard et al. 1981, Ann.
[0187] Rev. Biochem. 50:555-583. This nomenclature includes, for
instance, Man, which represents mannose; GlcNAc, which represents
2-N-acetylglucosamine; Gal which represents galactose; Fuc for
fucose; and Glc, which represents glucose. Sialic acids are
described by the shorthand notation NeuNAc, for
5-N-acetylneuraminic acid, and NeuNGc for 5-glycolylneuraminic.
[0188] The term "glycosylation" means the attachment of
oligosaccharides (carbohydrates containing two or more simple
sugars linked together e.g. from two to about twelve simple sugars
linked together) to a glycoprotein. The oligosaccharide side chains
are typically linked to the backbone of the glycoprotein through
either N- or O-linkages. The oligosaccharides of immunoglobulins
disclosed herein occur generally are attached to a CH2 domain of an
Fc region as N-linked oligosaccharides. "N-linked glycosylation"
refers to the attachment of the carbohydrate moiety to an
asparagine residue in a glycoprotein chain. The skilled artisan
will recognize that, for example, each of murine IgG1, IgG2a, IgG2b
and IgG3 as well as human IgG1, IgG2, IgG3, IgG4, IgA and IgD CH2
domains have a single site for N-linked glycosylation at amino acid
residue 297 (Kabat et al. Sequences of Proteins of Immunological
Interest, 1991).
[0189] For the purposes herein, a "mature core carbohydrate
structure" refers to a processed core carbohydrate structure
attached to an Fc region which generally consists of the following
carbohydrate structure GlcNAc(Fucose)-GlcNAc-Man-(Man-GlcNAc).sub.2
typical of biantennary oligosaccharides. The mature core
carbohydrate structure is attached to the Fc region of the
glycoprotein, generally via N-linkage to Asn297 of a CH2 domain of
the Fc region. A "bisecting GlcNAc" is a GlcNAc residue attached to
the .beta.1,4 mannose of the mature core carbohydrate structure.
The bisecting GlcNAc can be enzymatically attached to the mature
core carbohydrate structure by a
.beta.(1,4)-N-acetylglucosaminyltransferase III enzyme (GnTIII).
CHO cells do not normally express GnTIII (Stanley et al., 1984, J.
Biol. Chem. 261:13370-13378), but may be engineered to do so (Umana
et al., 1999, Nature Biotech. 17:176-180).
[0190] Described herein are Fc variants that comprise modified
glycoforms or engineered glycoforms. By "modified glycoform" or
"engineered glycoform" as used herein is meant a carbohydrate
composition that is covalently attached to a protein, for example
an antibody, wherein said carbohydrate composition differs
chemically from that of a parent protein. Engineered glycoforms may
be useful for a variety of purposes, including but not limited to
enhancing or reducing Fc.gamma.R-mediated effector function. In one
embodiment, the immunoglobulins disclosed herein are modified to
control the level of fucosylated and/or bisecting oligosaccharides
that are covalently attached to the Fc region.
[0191] A variety of methods are well known in the art for
generating modified glycoforms (Umana et al., 1999, Nat Biotechnol
17:176-180; Davies et al., 2001, Biotechnol Bioeng 74:288-294;
Shields et al., 2002, J Biol Chem 277:26733-26740; Shinkawa et al.,
2003, J Biol Chem 278:3466-3473); (U.S. Pat. No. 6,602,684; U.S.
Ser. No. 10/277,370; U.S. Ser. No. 10/113,929; PCT WO 00/61739A1;
PCT WO 01/29246A1; PCT WO 02/31140A1; PCT WO 02/30954A1);
(Potelligent.TM. technology [Biowa, Inc., Princeton, N.J.];
GlycoMAb.TM. glycosylation engineering technology [GLYCART
biotechnology AG, Zurich, Switzerland]; all of which are expressly
incorporated by reference).
[0192] These techniques control the level of fucosylated and/or
bisecting oligosaccharides that are covalently attached to the Fc
region, for example by expressing an IgG in various organisms or
cell lines, engineered or otherwise (for example Lec-13 CHO cells
or rat hybridoma YB2/0 cells), by regulating enzymes involved in
the glycosylation pathway (for example FUT8
[.alpha.1,6-fucosyltranserase] and/or
.beta.1-4-N-acetylglucosaminyltransferase III [GnTIII]), or by
modifying carbohydrate(s) after the IgG has been expressed. Other
methods for modifying glycoforms of the immunoglobulins disclosed
herein include using glycoengineered strains of yeast (Li et al.,
2006, Nature Biotechnology 24(2):210-215), moss (Nechansky et al.,
2007, Mol Immunjol 44(7):1826-8), and plants (Cox et al., 2006, Nat
Biotechnol 24(12):1591-7). The use of a particular method to
generate a modified glycoform is not meant to constrain embodiments
to that method. Rather, embodiments disclosed herein encompass Fc
variants with modified glycoforms irrespective of how they are
produced.
[0193] In one embodiment, immunoglobulins disclosed herein are
glycoengineered to alter the level of sialylation. Higher levels of
sialylated Fc glycans in immunoglobulin G molecules can adversely
impact functionality (Scallon et al., 2007, Mol Immunol.
44(7):1524-34), and differences in levels of Fc sialylation can
result in modified anti-inflammatory activity (Kaneko et al., 2006,
Science 313:670-673). Because antibodies may acquire
anti-inflammatory properties upon sialylation of Fc core
polysaccharide, it may be advantageous to glycoengineer the
immunoglobulins disclosed herein for greater or reduced Fc sialic
acid content.
[0194] Engineered glycoform typically refers to the different
carbohydrate or oligosaccharide; thus for example an immuoglobulin
may comprise an engineered glycoform. Alternatively, engineered
glycoform may refer to the immunoglobulin that comprises the
different carbohydrate or oligosaccharide. In one embodiment, a
composition disclosed herein comprises a glycosylated Fc variant
having an Fc region, wherein about 51-100% of the glycosylated
antibody, e.g., 80-100%, 90-100%, 95-100%, etc. of the antibody in
the composition comprises a mature core carbohydrate structure
which lacks fucose. In another embodiment, the antibody in the
composition both comprises a mature core carbohydrate structure
that lacks fucose and additionally comprises at least one amino
acid modification in the Fc region. In an alternative embodiment, a
composition comprises a glycosylated Fc variant having an Fc
region, wherein about 51-100% of the glycosylated antibody,
80-100%, or 90-100%, of the antibody in the composition comprises a
mature core carbohydrate structure which lacks sialic acid. In
another embodiment, the antibody in the composition both comprises
a mature core carbohydrate structure that lacks sialic acid and
additionally comprises at least one amino acid modification in the
Fc region. In yet another embodiment, a composition comprises a
glycosylated Fc variant having an Fc region, wherein about 51-100%
of the glycosylated antibody, 80-100%, or 90-100%, of the antibody
in the composition comprises a mature core carbohydrate structure
which contains sialic acid. In another embodiment, the antibody in
the composition both comprises a mature core carbohydrate structure
that contains sialic acid and additionally comprises at least one
amino acid modification in the Fc region. In another embodiment,
the combination of engineered glycoform and amino acid modification
provides optimal Fc receptor binding properties to the
antibody.
[0195] Other Modifications
[0196] Immunoglobulins disclosed herein may comprise one or more
modifications that provide optimized properties that are not
specifically related to Fc.gamma.R- or complement-mediated effector
functions per se. Said modifications may be amino acid
modifications, or may be modifications that are made enzymatically
or chemically. Such modification(s) likely provide some improvement
in the immunoglobulin, for example an enhancement in its stability,
solubility, function, or clinical use. Disclosed herein are a
variety of improvements that may be made by coupling the
immunoglobulins disclosed herein with additional modifications.
[0197] In one embodiment, the variable region of an antibody
disclosed herein may be affinity matured, that is to say that amino
acid modifications have been made in the VH and/or VL domains of
the antibody to enhance binding of the antibody to its target
antigen. Such types of modifications may improve the association
and/or the dissociation kinetics for binding to the target antigen.
Other modifications include those that improve selectivity for
target antigen vs. alternative targets. These include modifications
that improve selectivity for antigen expressed on target vs.
non-target cells. Other improvements to the target recognition
properties may be provided by additional modifications. Such
properties may include, but are not limited to, specific kinetic
properties (i.e. association and dissociation kinetics),
selectivity for the particular target versus alternative targets,
and selectivity for a specific form of target versus alternative
forms. Examples include full-length versus splice variants,
cell-surface vs. soluble forms, selectivity for various polymorphic
variants, or selectivity for specific conformational forms of the
target antigen. Immunoglobulins disclosed herein may comprise one
or more modifications that provide reduced or enhanced
internalization of an immunoglobulin.
[0198] In one embodiment, modifications are made to improve
biophysical properties of the immunoglobulins disclosed herein,
including but not limited to stability, solubility, and oligomeric
state. Modifications can include, for example, substitutions that
provide more favorable intramolecular interactions in the
immunoglobulin such as to provide greater stability, or
substitution of exposed nonpolar amino acids with polar amino acids
for higher solubility. A number of optimization goals and methods
are described in U.S. Ser. No. 10/379,392, incorporated entirely by
reference, that may find use for engineering additional
modifications to further optimize the immunoglobulins disclosed
herein. The immunoglobulins disclosed herein can also be combined
with additional modifications that reduce oligomeric state or size,
such that tumor penetration is enhanced, or in vivo clearance rates
are increased as desired.
[0199] Other modifications to the immunoglobulins disclosed herein
include those that enable the specific formation or homodimeric or
homomultimeric molecules. Such modifications include but are not
limited to engineered disulfides, as well as chemical modifications
or aggregation methods which may provide a mechanism for generating
covalent homodimeric or homomultimers. For example, methods of
engineering and compositions of such molecules are described in Kan
et al., 2001, J. Immunol., 2001, 166: 1320-1326; Stevenson et al.,
2002, Recent Results Cancer Res. 159: 104-12; U.S. Pat. No.
5,681,566; Caron et al., 1992, J. Exp. Med. 176:1191-1195, and
Shopes, 1992, J. Immunol. 148(9):2918-22, all incorporated enirely
by reference. Additional modifications to the variants disclosed
herein include those that enable the specific formation or
heterodimeric, heteromultimeric, bifunctional, and/or
multifunctional molecules. Such modifications include, but are not
limited to, one or more amino acid substitutions in the CH3 domain,
in which the substitutions reduce homodimer formation and increase
heterodimer formation. For example, methods of engineering and
compositions of such molecules are described in Atwell et al.,
1997, J. Mol. Biol. 270(1):26-35, and Carter et al., 2001, J.
Immunol. Methods 248:7-15, both incorporated entirely by reference.
Additional modifications include modifications in the hinge and CH3
domains, in which the modifications reduce the propensity to form
dimers.
[0200] In further embodiments, the immunoglobulins disclosed herein
comprise modifications that remove proteolytic degradation sites.
These may include, for example, protease sites that reduce
production yields, as well as protease sites that degrade the
administered protein in vivo. In one embodiment, additional
modifications are made to remove covalent degradation sites such as
deamidation (i.e. deamidation of glutaminyl and asparaginyl
residues to the corresponding glutamyl and aspartyl residues),
oxidation, and proteolytic degradation sites. Deamidation sites
that are particular useful to remove are those that have enhance
propensity for deamidation, including, but not limited to
asparaginyl and gltuamyl residues followed by glycines (NG and QG
motifs, respectively). In such cases, substitution of either
residue can significantly reduce the tendency for deamidation.
Common oxidation sites include methionine and cysteine residues.
Other covalent modifications, that can either be introduced or
removed, include hydroxylation of proline and lysine,
phosphorylation of hydroxyl groups of seryl or threonyl residues,
methylation of the "-amino groups of lysine, arginine, and
histidine side chains (T. E. Creighton, Proteins: Structure and
Molecular Properties, W.H. Freeman & Co., San Francisco, pp.
79-86 (1983), incorporated entirely by reference), acetylation of
the N-terminal amine, and amidation of any C-terminal carboxyl
group. Additional modifications also may include but are not
limited to posttranslational modifications such as N-linked or
O-linked glycosylation and phosphorylation.
[0201] Modifications may include those that improve expression
and/or purification yields from hosts or host cells commonly used
for production of biologics. These include, but are not limited to
various mammalian cell lines (e.g. CHO), yeast cell lines,
bacterial cell lines, and plants. Additional modifications include
modifications that remove or reduce the ability of heavy chains to
form inter-chain disulfide linkages. Additional modifications
include modifications that remove or reduce the ability of heavy
chains to form intra-chain disulfide linkages.
[0202] The immunoglobulins disclosed herein may comprise
modifications that include the use of unnatural amino acids
incorporated using, for example, the technologies developed by
Schultz and colleagues, including but not limited to methods
described by Cropp & Shultz, 2004, Trends Genet. 20(12):625-30,
Anderson et al., 2004, Proc. Natl. Acad. Sci. U.S.A. 101
(2):7566-71, Zhang et al., 2003, 303(5656):371-3, and Chin et al.,
2003, Science 301(5635):964-7, all incorporated entirely by
reference. In some embodiments, these modifications enable
manipulation of various functional, biophysical, immunological, or
manufacturing properties discussed above. In additional
embodiments, these modifications enable additional chemical
modification for other purposes. Other modifications are
contemplated herein. For example, the immunoglobulin may be linked
to one of a variety of nonproteinaceous polymers, e.g.,
polyethylene glycol (PEG), polypropylene glycol, polyoxyalkylenes,
or copolymers of polyethylene glycol and polypropylene glycol.
Additional amino acid modifications may be made to enable specific
or non-specific chemical or posttranslational modification of the
immunoglobulins. Such modifications, include, but are not limited
to PEGylation and glycosylation. Specific substitutions that can be
utilized to enable PEGylation include, but are not limited to,
introduction of novel cysteine residues or unnatural amino acids
such that efficient and specific coupling chemistries can be used
to attach a PEG or otherwise polymeric moiety. Introduction of
specific glycosylation sites can be achieved by introducing novel
N-X-T/S sequences into the immunoglobulins disclosed herein.
[0203] Modifications to reduce immunogenicity may include
modifications that reduce binding of processed peptides derived
from the parent sequence to MHC proteins. For example, amino acid
modifications would be engineered such that there are no or a
minimal number of immune epitopes that are predicted to bind, with
high affinity, to any prevalent MHC alleles. Several methods of
identifying MHC-binding epitopes in protein sequences are known in
the art and may be used to score epitopes in an antibody disclosed
herein. See for example U.S. Ser. No. 09/903,378, U.S. Ser. No.
10/754,296, U.S. Ser. No. 11/249,692, and references cited therein,
all expressly incorporated by reference.
[0204] In some embodiments, immunoglobulins disclosed herein may be
combined with immunoglobulins that alter FcRn binding. Such
variants may provide improved pharmacokinetic properties to the
immunoglobulins. In particular, variants that increase Fc binding
to FcRn include but are not limited to: 250E, 250Q, 428L, 428F,
250Q/428L (Hinton et al., 2004, J. Biol. Chem. 279(8): 6213-6216,
Hinton et al. 2006 Journal of Immunology 176:346-356, U.S. Ser. No.
11/102,621, PCT/US2003/033037, PCT/US2004/011213, U.S. Ser. No.
10/822,300, U.S. Ser. No. 10/687,118, PCT/US2004/034440, U.S. Ser.
No. 10/966,673 all entirely incorporated by reference), 256A, 272A,
286A, 305A, 307A, 311A, 312A, 376A, 378Q, 380A, 382A, 434A (Shields
et al, Journal of Biological Chemistry, 2001, 276(9):6591-6604,
U.S. Ser. No. 10/982,470, U.S. Pat. No. 6,737,056, U.S. Ser. No.
11/429,793, U.S. Ser. No. 11/429,786, PCT/US2005/029511, U.S. Ser.
No. 11/208,422, all entirely incorporated by reference), 252F,
252T, 252Y, 252W, 254T, 256S, 256R, 256Q, 256E, 256D, 256T, 309P,
311S, 433R, 433S, 4331, 433P, 433Q, 434H, 434F, 434Y,
252Y/254T/256E, 433K/434F/436H, 308T/309P/311 S (Dall Acqua et al.
Journal of Immunology, 2002, 169:5171-5180, U.S. Pat. No.
7,083,784, PCT/US97/03321, U.S. Pat. No. 6,821,505, PCT/US01/48432,
U.S. Ser. No. 11/397,328, all entirely incorporated by reference),
257C, 257M, 257L, 257N, 257Y, 279E, 279Q, 279Y, insertion of Ser
after 281, 283F, 284E, 306Y, 307V, 308F, 308Y 311 V, 385H, 385N,
(PCT/US2005/041220, U.S. Ser. No. 11/274,065, U.S. Ser. No.
11/436,266 all entirely incorporated by reference) 204D, 284E,
285E, 286D, and 290E (PCT/US2004/037929 entirely incorporated by
reference).
[0205] Covalent modifications of antibodies are included within the
scope of immunoglobulins disclosed herein, and are generally, but
not always, done post-translationally. For example, several types
of covalent modifications of the antibody are introduced into the
molecule by reacting specific amino acid residues of the antibody
with an organic derivatizing agent that is capable of reacting with
selected side chains or the N- or C-terminal residues.
[0206] In some embodiments, the covalent modification of the
antibodies disclosed herein comprises the addition of one or more
labels. The term "labeling group" means any detectable label. In
some embodiments, the labeling group is coupled to the antibody via
spacer arms of various lengths to reduce potential steric
hindrance. Various methods for labeling proteins are known in the
art and may be used in generating immunoglobulins disclosed herein.
In general, labels fall into a variety of classes, depending on the
assay in which they are to be detected: a) isotopic labels, which
may be radioactive or heavy isotopes; b) magnetic labels (e.g.,
magnetic particles); c) redox active moieties; d) optical dyes;
enzymatic groups (e.g. horseradish peroxidase,
.beta.-galactosidase, luciferase, alkaline phosphatase); e)
biotinylated groups; and f) predetermined polypeptide epitopes
recognized by a secondary reporter (e.g., leucine zipper pair
sequences, binding sites for secondary antibodies, metal binding
domains, epitope tags, etc.). In some embodiments, the labeling
group is coupled to the antibody via spacer arms of various lengths
to reduce potential steric hindrance. Various methods for labeling
proteins are known in the art and may be used in generating
immunoglobulins disclosed herein. Specific labels include optical
dyes, including, but not limited to, chromophores, phosphors and
fluorophores, with the latter being specific in many instances.
Fluorophores can be either "small molecule" fluores, or
proteinaceous fluores. By "fluorescent label" is meant any molecule
that may be detected via its inherent fluorescent properties.
[0207] Coniuqates
[0208] In one embodiment, the immunoglobulins disclosed herein are
antibody "fusion proteins", sometimes referred to herein as
"antibody conjugates". The fusion partner or conjugate partner can
be proteinaceous or non-proteinaceous; the latter generally being
generated using functional groups on the antibody and on the
conjugate partner. Conjugate and fusion partners may be any
molecule, including small molecule chemical compounds and
polypeptides. For example, a variety of antibody conjugates and
methods are described in Trail et al., 1999, Curr. Opin. Immunol.
11:584-588, incorporated entirely by reference. Possible conjugate
partners include but are not limited to cytokines, cytotoxic
agents, toxins, radioisotopes, chemotherapeutic agent,
anti-angiogenic agents, a tyrosine kinase inhibitors, and other
therapeutically active agents. In some embodiments, conjugate
partners may be thought of more as payloads, that is to say that
the goal of a conjugate is targeted delivery of the conjugate
partner to a targeted cell, for example a cancer cell or immune
cell, by the immunoglobulin. Thus, for example, the conjugation of
a toxin to an immunoglobulin targets the delivery of said toxin to
cells expressing the target antigen. As will be appreciated by one
skilled in the art, in reality the concepts and definitions of
fusion and conjugate are overlapping. The designation of a fusion
or conjugate is not meant to constrain it to any particular
embodiment disclosed herein. Rather, these terms are used loosely
to convey the broad concept that any immunoglobulin disclosed
herein may be linked genetically, chemically, or otherwise, to one
or more polypeptides or molecules to provide some desirable
property.
[0209] Suitable conjugates include, but are not limited to, labels
as described below, drugs and cytotoxic agents including, but not
limited to, cytotoxic drugs (e.g., chemotherapeutic agents) or
toxins or active fragments of such toxins. Suitable toxins and
their corresponding fragments include diptheria A chain, exotoxin A
chain, ricin A chain, abrin A chain, curcin, crotin, phenomycin,
enomycin and the like. Cytotoxic agents also include radiochemicals
made by conjugating radioisotopes to antibodies, or binding of a
radionuclide to a chelating agent that has been covalently attached
to the antibody. Additional embodiments utilize calicheamicin,
auristatins, geldanamycin, maytansine, and duocarmycins and
analogs; for the latter, see U.S. 2003/0050331, incorporated
enirely by reference.
[0210] In one embodiment, the immunoglobulins disclosed herein are
fused or conjugated to a cytokine. By "cytokine" as used herein is
meant a generic term for proteins released by one cell population
that act on another cell as intercellular mediators. For example,
as described in Penichet et al., 2001, J. Immunol. Methods
248:91-101, incorporated entirely by reference, cytokines may be
fused to antibody to provide an array of desirable properties.
Examples of such cytokines are lymphokines, monokines, and
traditional polypeptide hormones. Included among the cytokines are
growth hormone such as human growth hormone, N-methionyl human
growth hormone, and bovine growth hormone; parathyroid hormone;
thyroxine; insulin; proinsulin; relaxin; prorelaxin; glycoprotein
hormones such as follicle stimulating hormone (FSH), thyroid
stimulating hormone (TSH), and luteinizing hormone (LH); hepatic
growth factor; fibroblast growth factor; prolactin; placental
lactogen; tumor necrosis factor-alpha and -beta;
mullerian-inhibiting substance; mouse gonadotropin-associated
peptide; inhibin; activin; vascular endothelial growth factor;
integrin; thrombopoietin (TPO); nerve growth factors such as
NGF-beta; platelet-growth factor; transforming growth factors
(TGFs) such as TGF-alpha and TGF-beta; insulin-like growth factor-I
and -II; erythropoietin (EPO); osteoinductive factors; interferons
such as interferon-alpha, beta, and -gamma; colony stimulating
factors (CSFs) such as macrophage-CSF (M-CSF);
granulocyte-macrophage-CSF (GM-CSF); and granulocyte-CSF (G-CSF);
interleukins (ILs) such as IL-1, IL-1alpha, IL-2, IL-3, IL-4, IL-5,
IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12; IL-15, a tumor
necrosis factor such as TNF-alpha or TNF-beta; C5a; and other
polypeptide factors including LIF and kit ligand (KL). As used
herein, the term cytokine includes proteins from natural sources or
from recombinant cell culture, and biologically active equivalents
of the native sequence cytokines.
[0211] In an alternate embodiment, the immunoglobulins disclosed
herein are fused, conjugated, or operably linked to a toxin,
including but not limited to small molecule toxins and
enzymatically active toxins of bacterial, fungal, plant or animal
origin, including fragments and/or variants thereof. For example, a
variety of immunotoxins and immunotoxin methods are described in
Thrush et al., 1996, Ann. Rev. Immunol. 14:49-71, incorporated
entirely by reference. Small molecule toxins include but are not
limited to calicheamicin, maytansine (U.S. Pat. No. 5,208,020,
incorporated entirely by reference), trichothene, and CC1065. In
one embodiment, an immunoglobulin disclosed herein may be
conjugated to one or more maytansine molecules (e.g. about 1 to
about 10 maytansine molecules per antibody molecule). Maytansine
may, for example, be converted to May-SS-Me which may be reduced to
May-SH3 and reacted with modified antibody (Chari et al., 1992,
Cancer Research 52: 127-131, incorporated entirely by reference) to
generate a maytansinoid-antibody conjugate. Another conjugate of
interest comprises an immunoglobulin conjugated to one or more
calicheamicin molecules. The calicheamicin family of antibiotics
are capable of producing double-stranded DNA breaks at
sub-picomolar concentrations. Structural analogues of calicheamicin
that may be used include but are not limited to
.gamma..sub.1.sup.1, .alpha..sub.2.sup.1, .alpha..sub.3,
N-acetyl-.gamma..sub.1.sup.1, PSAG, and .THETA..sub.1.sup.1,
(Hinman et al., 1993, Cancer Research 53:3336-3342; Lode et al.,
1998, Cancer Research 58:2925-2928) (U.S. Pat. No. 5,714,586; U.S.
Pat. No. 5,712,374; U.S. Pat. No. 5,264,586; U.S. Pat. No.
5,773,001, all incorporated enirely by reference). Dolastatin 10
analogs such as auristatin E (AE) and monomethylauristatin E (MMAE)
may find use as conjugates for the immunoglobulins disclosed herein
(Doronina et al., 2003, Nat Biotechnol 21(7):778-84; Francisco et
al., 2003 Blood 102(4):1458-65, both incorporated entirely by
reference). Useful enyzmatically active toxins include but are not
limited to diphtheria A chain, nonbinding active fragments of
diphtheria toxin, exotoxin A chain (from Pseudomonas aeruginosa),
ricin A chain, abrin A chain, modeccin A chain, alpha-sarcin,
Aleurites fordii proteins, dianthin proteins, Phytolaca americana
proteins (PAPI, PAPII, and PAP-S), momordica charantia inhibitor,
curcin, crotin, sapaonaria officinalis inhibitor, gelonin,
mitogellin, restrictocin, phenomycin, enomycin and the
tricothecenes. See, for example, PCT WO 93/21232, incorporated
entirely by reference. Embodiments further encompass a conjugate
between an immunoglobulin disclosed herein and a compound with
nucleolytic activity, for example a ribonuclease or DNA
endonuclease such as a deoxyribonuclease (DNase).
[0212] In an alternate embodiment, an immunoglobulin disclosed
herein may be fused, conjugated, or operably linked to a
radioisotope to form a radioconjugate. A variety of radioactive
isotopes are available for the production of radioconjugate
antibodies. Examples include, but are not limited to, At211, I131,
I125, Y90, Re186, Re188, Sm153, Bi212, P32, and radioactive
isotopes of Lu. See for example, reference.
[0213] In yet another embodiment, an immunoglobulin disclosed
herein may be conjugated to a "receptor" (such streptavidin) for
utilization in tumor pretargeting wherein the
immunoglobulin-receptor conjugate is administered to the patient,
followed by removal of unbound conjugate from the circulation using
a clearing agent and then administration of a "ligand" (e.g.
avidin) which is conjugated to a cytotoxic agent (e.g. a
radionucleotide). In an alternate embodiment, the immunoglobulin is
conjugated or operably linked to an enzyme in order to employ
Antibody Dependent Enzyme Mediated Prodrug Therapy (ADEPT). ADEPT
may be used by conjugating or operably linking the immunoglobulin
to a prodrug-activating enzyme that converts a prodrug (e.g. a
peptidyl chemotherapeutic agent, see PCT WO 81/01145, incorporated
entirely by reference) to an active anti-cancer drug. See, for
example, PCT WO 88/07378 and U.S. Pat. No. 4,975,278, both
incorporated entirely by reference. The enzyme component of the
immunoconjugate useful for ADEPT includes any enzyme capable of
acting on a prodrug in such a way so as to covert it into its more
active, cytotoxic form. Enzymes that are useful in the method
disclosed herein include but are not limited to alkaline
phosphatase useful for converting phosphate-containing prodrugs
into free drugs; arylsulfatase useful for converting
sulfate-containing prodrugs into free drugs; cytosine deaminase
useful for converting non-toxic 5-fluorocytosine into the
anti-cancer drug, 5-fluorouracil; proteases, such as serratia
protease, thermolysin, subtilisin, carboxypeptidases and cathepsins
(such as cathepsins B and L), that are useful for converting
peptide-containing prodrugs into free drugs;
D-alanylcarboxypeptidases, useful for converting prodrugs that
contain D-amino acid substituents; carbohydrate-cleaving enzymes
such as .beta.-galactosidase and neuramimidase useful for
converting glycosylated prodrugs into free drugs; beta-lactamase
useful for converting drugs derivatized with .alpha.-lactams into
free drugs; and penicillin amidases, such as penicillin V amidase
or penicillin G amidase, useful for converting drugs derivatized at
their amine nitrogens with phenoxyacetyl or phenylacetyl groups,
respectively, into free drugs. Alternatively, antibodies with
enzymatic activity, also known in the art as "abzymes", can be used
to convert the prodrugs disclosed herein into free active drugs
(see, for example, Massey, 1987, Nature 328: 457-458, incorporated
entirely by reference). immunoglobulin-abzyme conjugates can be
prepared for delivery of the abzyme to a tumor cell population. A
variety of additional conjugates are contemplated for the
immunoglobulins disclosed herein. A variety of chemotherapeutic
agents, anti-angiogenic agents, tyrosine kinase inhibitors, and
other therapeutic agents are described below, which may find use as
immunoglobulin conjugates.
[0214] Conjugate partners may be linked to any region of an
immunoglobulin disclosed herein, including at the N- or C-termini,
or at some residue in-between the termini. A variety of linkers may
find use in immunoglobulins disclosed herein to covalently link
conjugate partners to an immunoglobulin. By "linker", "linker
sequence", "spacer", "tethering sequence" or grammatical
equivalents thereof, herein is meant a molecule or group of
molecules (such as a monomer or polymer) that connects two
molecules and often serves to place the two molecules in one
configuration. Linkers are known in the art; for example, homo- or
hetero-bifunctional linkers as are well known (see, 1994 Pierce
Chemical Company catalog, technical section on cross-linkers, pages
155-200, incorporated entirely by reference). A number of
strategies may be used to covalently link molecules together. These
include, but are not limited to polypeptide linkages between N- and
C-termini of proteins or protein domains, linkage via disulfide
bonds, and linkage via chemical cross-linking reagents. In one
aspect of this embodiment, the linker is a peptide bond, generated
by recombinant techniques or peptide synthesis. The linker peptide
may predominantly include the following amino acid residues: Gly,
Ser, Ala, or Thr. The linker peptide should have a length that is
adequate to link two molecules in such a way that they assume the
correct conformation relative to one another so that they retain
the desired activity. Suitable lengths for this purpose include at
least one and not more than 50 amino acid residues. In one
embodiment, the linker is from about 1 to 30 amino acids in length,
e.g., a linker may be 1 to 20 amino acids in length. Useful linkers
include glycine-serine polymers (including, for example, (GS)n,
(GSGGS)n (Set forth as SEQ ID NO:1), (GGGGS)n (Set forth as SEQ ID
NO:2) and (GGGS)n (Set forth as SEQ ID NO:3), where n is an integer
of at least one), glycine-alanine polymers, alanine-serine
polymers, and other flexible linkers, as will be appreciated by
those in the art. Alternatively, a variety of nonproteinaceous
polymers, including but not limited to polyethylene glycol (PEG),
polypropylene glycol, polyoxyalkylenes, or copolymers of
polyethylene glycol and polypropylene glycol, may find use as
linkers.
[0215] Production of Immunoqlobulins
[0216] Also disclosed herein are methods for producing and
experimentally testing immunoglobulins. The disclosed methods are
not meant to constrain embodiments to any particular application or
theory of operation. Rather, the provided methods are meant to
illustrate generally that one or more immunoglobulins may be
produced and experimentally tested to obtain immunoglobulins.
General methods for antibody molecular biology, expression,
purification, and screening are described in Antibody Engineering,
edited by Duebel & Kontermann, Springer-Verlag, Heidelberg,
2001; and Hayhurst & Georgiou, 2001, Curr Opin Chem Biol
5:683-689; Maynard & Georgiou, 2000, Annu Rev Biomed Eng
2:339-76; Antibodies: A Laboratory Manual by Harlow & Lane, New
York: Cold Spring Harbor Laboratory Press, 1988, all incorporated
enirely by reference.
[0217] In one embodiment disclosed herein, nucleic acids are
created that encode the immunoglobulins, and that may then be
cloned into host cells, expressed and assayed, if desired. Thus,
nucleic acids, and particularly DNA, may be made that encode each
protein sequence. These practices are carried out using well-known
procedures. For example, a variety of methods that may find use in
generating immunoglobulins disclosed herein are described in
Molecular Cloning--A Laboratory Manual, 3.sup.rd Ed. (Maniatis,
Cold Spring Harbor Laboratory Press, New York, 2001), and Current
Protocols in Molecular Biology (John Wiley & Sons), both
incorporated entirely by reference. As will be appreciated by those
skilled in the art, the generation of exact sequences for a library
comprising a large number of sequences is potentially expensive and
time consuming. By "library" herein is meant a set of variants in
any form, including but not limited to a list of nucleic acid or
amino acid sequences, a list of nucleic acid or amino acid
substitutions at variable positions, a physical library comprising
nucleic acids that encode the library sequences, or a physical
library comprising the variant proteins, either in purified or
unpurified form. Accordingly, there are a variety of techniques
that may be used to efficiently generate libraries disclosed
herein. Such methods that may find use for generating
immunoglobulins disclosed herein are described or referenced in
U.S. Pat. No. 6,403,312; U.S. Ser. No. 09/782,004; U.S. Ser. No.
09/927,790; U.S. Ser. No. 10/218,102; PCT WO 01/40091; and PCT WO
02/25588,all incorporated entirely by reference. Such methods
include but are not limited to gene assembly methods, PCR-based
method and methods which use variations of PCR, ligase chain
reaction-based methods, pooled oligo methods such as those used in
synthetic shuffling, error-prone amplification methods and methods
which use oligos with random mutations, classical site-directed
mutagenesis methods, cassette mutagenesis, and other amplification
and gene synthesis methods. As is known in the art, there are a
variety of commercially available kits and methods for gene
assembly, mutagenesis, vector subcloning, and the like, and such
commercial products find use in for generating nucleic acids that
encode immunoglobulins.
[0218] The immunoglobulins disclosed herein may be produced by
culturing a host cell transformed with nucleic acid, e.g., an
expression vector, containing nucleic acid encoding the
immunoglobulins, under the appropriate conditions to induce or
cause expression of the protein.
[0219] The conditions appropriate for expression will vary with the
choice of the expression vector and the host cell, and will be
easily ascertained by one skilled in the art through routine
experimentation. A wide variety of appropriate host cells may be
used, including but not limited to mammalian cells, bacteria,
insect cells, and yeast. For example, a variety of cell lines that
may find use in generating immunoglobulins disclosed herein are
described in the ATCC.RTM. cell line catalog, available from the
American Type Culture Collection.
[0220] In one embodiment, the immunoglobulins are expressed in
mammalian expression systems, including systems in which the
expression constructs are introduced into the mammalian cells using
virus such as retrovirus or adenovirus. Any mammalian cells may be
used, e.g., human, mouse, rat, hamster, and primate cells. Suitable
cells also include known research cells, including but not limited
to Jurkat T cells, NIH3T3, CHO, BHK, COS, HEK293, PER C.6, HeLa,
Sp2/0, NS0 cells and variants thereof. In an alternate embodiment,
library proteins are expressed in bacterial cells. Bacterial
expression systems are well known in the art, and include
Escherichia coli (E. coli), Bacillus subtilis, Streptococcus
cremoris, and Streptococcus lividans. In alternate embodiments,
immunoglobulins are produced in insect cells (e.g. Sf21/Sf9,
Trichoplusia ni Bti-Tn5b1-4) or yeast cells (e.g. S. cerevisiae,
Pichia, etc). In an alternate embodiment, immunoglobulins are
expressed in vitro using cell free translation systems. In vitro
translation systems derived from both prokaryotic (e.g. E. coli)
and eukaryotic (e.g. wheat germ, rabbit reticulocytes) cells are
available and may be chosen based on the expression levels and
functional properties of the protein of interest. For example, as
appreciated by those skilled in the art, in vitro translation is
required for some display technologies, for example ribosome
display. In addition, the immunoglobulins may be produced by
chemical synthesis methods. Also transgenic expression systems both
animal (e.g. cow, sheep or goat milk, embryonated hen's eggs, whole
insect larvae, etc.) and plant (e.g. corn, tobacco, duckweed,
etc.)
[0221] The nucleic acids that encode the immunoglobulins disclosed
herein may be incorporated into an expression vector in order to
express the protein. A variety of expression vectors may be
utilized for protein expression. Expression vectors may comprise
self-replicating extra-chromosomal vectors or vectors which
integrate into a host genome. Expression vectors are constructed to
be compatible with the host cell type. Thus expression vectors
which find use in generating immunoglobulins disclosed herein
include but are not limited to those which enable protein
expression in mammalian cells, bacteria, insect cells, yeast, and
in in vitro systems. As is known in the art, a variety of
expression vectors are available, commercially or otherwise, that
may find use for expressing immunoglobulins disclosed herein.
[0222] Expression vectors typically comprise a protein operably
linked with control or regulatory sequences, selectable markers,
any fusion partners, and/or additional elements. By "operably
linked" herein is meant that the nucleic acid is placed into a
functional relationship with another nucleic acid sequence.
Generally, these expression vectors include transcriptional and
translational regulatory nucleic acid operably linked to the
nucleic acid encoding the immunoglobulin, and are typically
appropriate to the host cell used to express the protein. In
general, the transcriptional and translational regulatory sequences
may include promoter sequences, ribosomal binding sites,
transcriptional start and stop sequences, translational start and
stop sequences, and enhancer or activator sequences. As is also
known in the art, expression vectors typically contain a selection
gene or marker to allow the selection of transformed host cells
containing the expression vector. Selection genes are well known in
the art and will vary with the host cell used.
[0223] Immunoglobulins may be operably linked to a fusion partner
to enable targeting of the expressed protein, purification,
screening, display, and the like. Fusion partners may be linked to
the immunoglobulin sequence via a linker sequences. The linker
sequence will generally comprise a small number of amino acids,
typically less than ten, although longer linkers may also be used.
Typically, linker sequences are selected to be flexible and
resistant to degradation. As will be appreciated by those skilled
in the art, any of a wide variety of sequences may be used as
linkers. For example, a common linker sequence comprises the amino
acid sequence GGGGS. A fusion partner may be a targeting or signal
sequence that directs immunoglobulin and any associated fusion
partners to a desired cellular location or to the extracellular
media. As is known in the art, certain signaling sequences may
target a protein to be either secreted into the growth media, or
into the periplasmic space, located between the inner and outer
membrane of the cell. A fusion partner may also be a sequence that
encodes a peptide or protein that enables purification and/or
screening. Such fusion partners include but are not limited to
polyhistidine tags (His-tags) (for example H.sub.6 and H.sub.10 or
other tags for use with Immobilized Metal Affinity Chromatography
(IMAC) systems (e.g. Ni.sup.+2 affinity columns)), GST fusions, MBP
fusions, Strep-tag, the BSP biotinylation target sequence of the
bacterial enzyme BirA, and epitope tags which are targeted by
antibodies (for example c-myc tags, flag-tags, and the like). As
will be appreciated by those skilled in the art, such tags may be
useful for purification, for screening, or both. For example, an
immunoglobulin may be purified using a His-tag by immobilizing it
to a Ni.sup.+2 affinity column, and then after purification the
same His-tag may be used to immobilize the antibody to a Ni.sup.+2
coated plate to perform an ELISA or other binding assay (as
described below). A fusion partner may enable the use of a
selection method to screen immunoglobulins (see below). Fusion
partners that enable a variety of selection methods are well-known
in the art. For example, by fusing the members of an immunoglobulin
library to the gene III protein, phage display can be employed (Kay
et al., Phage display of peptides and proteins: a laboratory
manual, Academic Press, San Diego, Calif., 1996; Lowman et al.,
1991, Biochemistry 30:10832-10838; Smith, 1985, Science
228:1315-1317, incorporated entirely by reference). Fusion partners
may enable immunoglobulins to be labeled. Alternatively, a fusion
partner may bind to a specific sequence on the expression vector,
enabling the fusion partner and associated immunoglobulin to be
linked covalently or noncovalently with the nucleic acid that
encodes them. The methods of introducing exogenous nucleic acid
into host cells are well known in the art, and will vary with the
host cell used. Techniques include but are not limited to
dextran-mediated transfection, calcium phosphate precipitation,
calcium chloride treatment, polybrene mediated transfection,
protoplast fusion, electroporation, viral or phage infection,
encapsulation of the polynucleotide(s) in liposomes, and direct
microinjection of the DNA into nuclei. In the case of mammalian
cells, transfection may be either transient or stable.
[0224] In one embodiment, immunoglobulins are purified or isolated
after expression. Proteins may be isolated or purified in a variety
of ways known to those skilled in the art. Standard purification
methods include chromatographic techniques, including ion exchange,
hydrophobic interaction, affinity, sizing or gel filtration, and
reversed-phase, carried out at atmospheric pressure or at high
pressure using systems such as FPLC and HPLC. Purification methods
also include electrophoretic, immunological, precipitation,
dialysis, and chromatofocusing techniques. Ultrafiltration and
diafiltration techniques, in conjunction with protein
concentration, are also useful. As is well known in the art, a
variety of natural proteins bind Fc and antibodies, and these
proteins can find use for purification of immunoglobulins disclosed
herein. For example, the bacterial proteins A and G bind to the Fc
region. Likewise, the bacterial protein L binds to the Fab region
of some antibodies, as of course does the antibody's target
antigen. Purification can often be enabled by a particular fusion
partner. For example, immunoglobulins may be purified using
glutathione resin if a GST fusion is employed, Ni.sup.+2 affinity
chromatography if a His-tag is employed, or immobilized anti-flag
antibody if a flag-tag is used. For general guidance in suitable
purification techniques, see, e.g. incorporated entirely by
reference Protein Purification: Principles and Practice, 3.sup.rd
Ed., Scopes, Springer-Verlag, NY, 1994, incorporated entirely by
reference. The degree of purification necessary will vary depending
on the screen or use of the immunoglobulins. In some instances no
purification is necessary. For example in one embodiment, if the
immunoglobulins are secreted, screening may take place directly
from the media. As is well known in the art, some methods of
selection do not involve purification of proteins. Thus, for
example, if a library of immunoglobulins is made into a phage
display library, protein purification may not be performed.
[0225] In Vitro Experimentation
[0226] Immunoglobulins may be screened using a variety of methods,
including but not limited to those that use in vitro assays, in
vivo and cell-based assays, and selection technologies.
[0227] Automation and high-throughput screening technologies may be
utilized in the screening procedures. Screening may employ the use
of a fusion partner or label. The use of fusion partners has been
discussed above. By "labeled" herein is meant that the
immunoglobulins disclosed herein have one or more elements,
isotopes, or chemical compounds attached to enable the detection in
a screen. In general, labels fall into three classes: a) immune
labels, which may be an epitope incorporated as a fusion partner
that is recognized by an antibody, b) isotopic labels, which may be
radioactive or heavy isotopes, and c) small molecule labels, which
may include fluorescent and colorimetric dyes, or molecules such as
biotin that enable other labeling methods. Labels may be
incorporated into the compound at any position and may be
incorporated in vitro or in vivo during protein expression.
[0228] In one embodiment, the functional and/or biophysical
properties of immunoglobulins are screened in an in vitro assay. In
vitro assays may allow a broad dynamic range for screening
properties of interest. Properties of immunoglobulins that may be
screened include but are not limited to stability, solubility, and
affinity for Fc ligands, for example Fc.gamma.Rs. Multiple
properties may be screened simultaneously or individually. Proteins
may be purified or unpurified, depending on the requirements of the
assay. In one embodiment, the screen is a qualitative or
quantitative binding assay for binding of immunoglobulins to a
protein or nonprotein molecule that is known or thought to bind the
immunoglobulin. In one embodiment, the screen is a binding assay
for measuring binding to the target antigen. In an alternate
embodiment, the screen is an assay for binding of immunoglobulins
to an Fc ligand, including but are not limited to the family of
Fc.gamma.Rs, the neonatal receptor FcRn, the complement protein
C1q, and the bacterial proteins A and G. Said Fc ligands may be
from any organism. In one embodiment, Fc ligands are from humans,
mice, rats, rabbits, and/or monkeys. Binding assays can be carried
out using a variety of methods known in the art, including but not
limited to FRET (Fluorescence Resonance Energy Transfer) and BRET
(Bioluminescence Resonance Energy Transfer) -based assays,
AlphaScreen.TM. (Amplified Luminescent Proximity Homogeneous
Assay), Scintillation Proximity Assay, ELISA (Enzyme-Linked
Immunosorbent Assay), SPR (Surface Plasmon Resonance, also known as
BIACORE.RTM.), isothermal titration calorimetry, differential
scanning calorimetry, gel electrophoresis, and chromatography
including gel filtration. These and other methods may take
advantage of some fusion partner or label of the immunoglobulin.
Assays may employ a variety of detection methods including but not
limited to chromogenic, fluorescent, luminescent, or isotopic
labels.
[0229] The biophysical properties of immunoglobulins, for example
stability and solubility, may be screened using a variety of
methods known in the art. Protein stability may be determined by
measuring the thermodynamic equilibrium between folded and unfolded
states. For example, immunoglobulins disclosed herein may be
unfolded using chemical denaturant, heat, or pH, and this
transition may be monitored using methods including but not limited
to circular dichroism spectroscopy, fluorescence spectroscopy,
absorbance spectroscopy, NMR spectroscopy, calorimetry, and
proteolysis. As will be appreciated by those skilled in the art,
the kinetic parameters of the folding and unfolding transitions may
also be monitored using these and other techniques. The solubility
and overall structural integrity of an immunoglobulin may be
quantitatively or qualitatively determined using a wide range of
methods that are known in the art. Methods which may find use for
characterizing the biophysical properties of immunoglobulins
disclosed herein include gel electrophoresis, isoelectric focusing,
capillary electrophoresis, chromatography such as size exclusion
chromatography, ion-exchange chromatography, and reversed-phase
high performance liquid chromatography, peptide mapping,
oligosaccharide mapping, mass spectrometry, ultraviolet absorbance
spectroscopy, fluorescence spectroscopy, circular dichroism
spectroscopy, isothermal titration calorimetry, differential
scanning calorimetry, analytical ultra-centrifugation, dynamic
light scattering, proteolysis, and cross-linking, turbidity
measurement, filter retardation assays, immunological assays,
fluorescent dye binding assays, protein-staining assays,
microscopy, and detection of aggregates via ELISA or other binding
assay. Structural analysis employing X-ray crystallographic
techniques and NMR spectroscopy may also find use. In one
embodiment, stability and/or solubility may be measured by
determining the amount of protein solution after some defined
period of time. In this assay, the protein may or may not be
exposed to some extreme condition, for example elevated
temperature, low pH, or the presence of denaturant. Because
function typically requires a stable, soluble, and/or
well-folded/structured protein, the aforementioned functional and
binding assays also provide ways to perform such a measurement. For
example, a solution comprising an immunoglobulin could be assayed
for its ability to bind target antigen, then exposed to elevated
temperature for one or more defined periods of time, then assayed
for antigen binding again. Because unfolded and aggregated protein
is not expected to be capable of binding antigen, the amount of
activity remaining provides a measure of the immunoglobulin's
stability and solubility.
[0230] In one embodiment, the library is screened using one or more
cell-based or in vitro assays. For such assays, immunoglobulins,
purified or unpurified, are typically added exogenously such that
cells are exposed to individual variants or groups of variants
belonging to a library. These assays are typically, but not always,
based on the biology of the ability of the immunoglobulin to bind
to the target antigen and mediate some biochemical event, for
example effector functions like cellular lysis, phagocytosis,
ligand/receptor binding inhibition, inhibition of growth and/or
proliferation, apoptosisand the like. Such assays often involve
monitoring the response of cells to immunoglobulin, for example
cell survival, cell death, cellular phagocytosis, cell lysis,
change in cellular morphology, or transcriptional activation such
as cellular expression of a natural gene or reporter gene. For
example, such assays may measure the ability of immunoglobulins to
elicit ADCC, ADCP, or CDC. For some assays additional cells or
components, that is in addition to the target cells, may need to be
added, for example serum complement, or effector cells such as
peripheral blood monocytes (PBMCs), NK cells, macrophages, and the
like. Such additional cells may be from any organism, e.g., humans,
mice, rat, rabbit, and monkey. Crosslinked or monomeric antibodies
may cause apoptosis of certain cell lines expressing the antibody's
target antigen, or they may mediate attack on target cells by
immune cells which have been added to the assay. Methods for
monitoring cell death or viability are known in the art, and
include the use of dyes, fluorophores, immunochemical,
cytochemical, and radioactive reagents. For example, caspase assays
or annexin-flourconjugates may enable apoptosis to be measured, and
uptake or release of radioactive substrates (e.g. Chromium-51
release assays) or the metabolic reduction of fluorescent dyes such
as alamar blue may enable cell growth, proliferation or activation
to be monitored. In one embodiment, the DELFIA.RTM. EuTDA-based
cytotoxicity assay (Perkin Elmer, MA) is used. Alternatively, dead
or damaged target cells may be monitored by measuring the release
of one or more natural intracellular proteins, for example lactate
dehydrogenase. Transcriptional activation may also serve as a
method for assaying function in cell-based assays. In this case,
response may be monitored by assaying for natural genes or proteins
which may be upregulated or down-regulated, for example the release
of certain interleukins may be measured, or alternatively readout
may be via a luciferase or GFP-reporter construct. Cell-based
assays may also involve the measure of morphological changes of
cells as a response to the presence of an immunoglobulin. Cell
types for such assays may be prokaryotic or eukaryotic, and a
variety of cell lines that are known in the art may be employed.
Alternatively, cell-based screens are performed using cells that
have been transformed or transfected with nucleic acids encoding
the immunoglobulins.
[0231] In vitro assays include but are not limited to binding
assays, ADCC, CDC, cytotoxicity, proliferation, peroxide/ozone
release, chemotaxis of effector cells, inhibition of such assays by
reduced effector function antibodies; ranges of activities such as
>100.times. improvement or >100.times. reduction, blends of
receptor activation and the assay outcomes that are expected from
such receptor profiles.
[0232] In Vivo Experimentation
[0233] The biological properties of the immunoglobulins disclosed
herein may be characterized in cell, tissue, and whole organism
experiments. As is known in the art, drugs are often tested in
animals, including but not limited to mice, rats, rabbits, dogs,
cats, pigs, and monkeys, in order to measure a drug's efficacy for
treatment against a disease or disease model, or to measure a
drug's pharmacokinetics, toxicity, and other properties. Said
animals may be referred to as disease models. With respect to the
immunoglobulins disclosed herein, a particular challenge arises
when using animal models to evaluate the potential for in-human
efficacy of candidate polypeptides--this is due, at least in part,
to the fact that immunoglobulins that have a specific effect on the
affinity for a human Fc receptor may not have a similar affinity
effect with the orthologous animal receptor. These problems can be
further exacerbated by the inevitable ambiguities associated with
correct assignment of true orthologues (Mechetina et al.,
Immunogenetics, 2002 54:463-468, incorporated entirely by
reference), and the fact that some orthologues simply do not exist
in the animal (e.g. humans possess an Fc.gamma.RIIa whereas mice do
not). Therapeutics are often tested in mice, including but not
limited to mouse strains NZB, NOD, BXSB, MRL/Ipr, K/B.times.N and
transgenics (including knockins and knockouts). Such mice can
develop various autoimmune conditions that resemble human organ
specific, systemic autoimmune or inflammatory disease pathologies
such as systemic lupus erythematosus (SLE) and rheumatoid arthritis
(RA). For example, an immunoglobulin disclosed herein intended for
autoimmune diseases may be tested in such mouse models by treating
the mice to determine the ability of the immunoglobulin to reduce
or inhibit the development of the disease pathology.
[0234] Because of the incompatibility between the mouse and human
Fc.gamma. receptor system, an alternative approach is to use a
murine SCID model in which immune deficient mice are engrafted with
human PBLs or PBMCs (huPBL-SCID, huPBMC-SCID) providing a
semi-functional human immune system with human effector cells and
Fc receptors. In such a model, an antigen challenge (such as
tetanus toxoid) activates the B cells to differentiate into plasma
cells and secrete immunoglobulins, thus reconstituting antigen
specific humoral immunity. Therefore, a dual targeting
immunoglobulin disclosed herein that specifically binds to an
antigen (such as CD19 or CD79a/b) and Fc.gamma.RIIb on B cells may
be tested to examine the ability to specifically inhibit B cell
differentiation. Such experimentation may provide meaningful data
for determination of the potential of said immunoglobulin to be
used as a therapeutic. Other organisms, e.g., mammals, may also be
used for testing. For example, because of their genetic similarity
to humans, monkeys can be suitable therapeutic models, and thus may
be used to test the efficacy, toxicity, pharmacokinetics, or other
property of the immunoglobulins disclosed herein. Tests of the
immunoglobulins disclosed herein in humans are ultimately required
for approval as drugs, and thus of course these experiments are
contemplated. Thus the immunoglobulins disclosed herein may be
tested in humans to determine their therapeutic efficacy, toxicity,
pharmacokinetics, and/or other clinical properties.
[0235] The immunoglobulins disclosed herein may confer superior
performance on Fc-containing therapeutics in animal models or in
humans. The receptor binding profiles of such immunoglobulins, as
described in this specification, may, for example, be selected to
increase the potency of cytotoxic drugs or to target specific
effector functions or effector cells to improve the selectivity of
the drug's action. Further, receptor binding profiles can be
selected that may reduce some or all effector functions thereby
reducing the side-effects or toxicity of such Fc-containing drug.
For example, an immunoglobulin with reduced binding to
Fc.gamma.RIIIa, Fc.gamma.RI and Fc.gamma.RIIa can be selected to
eliminate most cell-mediated effector function, or an
immunoglobulin with reduced binding to C1q may be selected to limit
complement-mediated effector functions. In some contexts, such
effector functions are known to have potential toxic effects.
Therefore eliminating them may increase the safety of the
Fc-bearing drug and such improved safety may be characterized in
animal models. In some contexts, such effector functions are known
to mediate the desirable therapeutic activity. Therefore enhancing
them may increase the activity or potency of the Fc-bearing drug
and such improved activity or potency may be characterized in
animal models.
[0236] In some embodiments, immunoglobulins disclosed herein may be
assessed for efficacy in clinically relevant animal models of
various human diseases. In many cases, relevant models include
various transgenic animals for specific antigens and receptors.
[0237] Relevant transgenic models such as those that express human
Fc receptors (e.g., CD32b) could be used to evaluate and test
immunoglobulins and Fc-fusions in their efficacy. The evaluation of
immunoglobulins by the introduction of human genes that directly or
indirectly mediate effector function in mice or other rodents may
enable physiological studies of efficacy in autoimmune disorders
and RA. Human Fc receptors such as Fc.gamma.RIIb may possess
polymorphisms such as that in gene promoter (-343 from G to C) or
transmembrane domain of the receptor 187 I or T which would further
enable the introduction of specific and combinations of human
polymorphisms into rodents. The various studies involving
polymorphism-specific FcRs is not limited to this section, however
encompasses all discussions and applications of FcRs in general as
specified in throughout this application. Immunoglobulins disclosed
herein may confer superior activity on Fc-containing drugs in such
transgenic models, in particular variants with binding profiles
optimized for human Fc.gamma.RIIb mediated activity may show
superior activity in transgenic CD32b mice. Similar improvements in
efficacy in mice transgenic for the other human Fc receptors, e.g.
Fc.gamma.RIIa, Fc.gamma.RI, etc., may be observed for
immunoglobulins with binding profiles optimized for the respective
receptors. Mice transgenic for multiple human receptors would show
improved activity for immunoglobulins with binding profiles
optimized for the corresponding multiple receptors.
[0238] Because of the difficulties and ambiguities associated with
using animal models to characterize the potential efficacy of
candidate therapeutic antibodies in a human patient, some variant
polypeptides disclosed herein may find utility as proxies for
assessing potential in-human efficacy. Such proxy molecules may
mimic--in the animal system--the FcR and/or complement biology of a
corresponding candidate human immunoglobulin. This mimicry is most
likely to be manifested by relative association affinities between
specific immunoglobulins and animal vs. human receptors. For
example, if one were using a mouse model to assess the potential
in-human efficacy of an Fc variant that has reduced affinity for
the inhibitory human Fc.gamma.RIIb, an appropriate proxy variant
would have reduced affinity for mouse Fc.gamma.RII. It should also
be noted that the proxy Fc variants could be created in the context
of a human Fc variant, an animal Fc variant, or both.
[0239] In one embodiment, the testing of immunoglobulins may
include study of efficacy in primates (e.g. cynomolgus monkey
model) to facilitate the evaluation of depletion of specific target
cells harboring the target antigen. Additional primate models
include but are not limited to use of the rhesus monkey to assess
Fc polypeptides in therapeutic studies of autoimmune,
transplantation and cancer.
[0240] Toxicity studies are performed to determine antibody or
Fc-fusion related-effects that cannot be evaluated in standard
pharmacology profiles, or occur only after repeated administration
of the agent. Most toxicity tests are performed in two species--a
rodent and a non-rodent--to ensure that any unexpected adverse
effects are not overlooked before new therapeutic entities are
introduced into man. In general, these models may measure a variety
of toxicities including genotoxicity, chronic toxicity,
immunogenicity, reproductive/developmental toxicity and
carcinogenicity. Included within the aforementioned parameters are
standard measurement of food consumption, bodyweight, antibody
formation, clinical chemistry, and macro- and microscopic
examination of standard organs/tissues (e.g. cardiotoxicity).
Additional parameters of measurement are injection site trauma and
the measurement of neutralizing antibodies, if any. Traditionally,
monoclonal antibody therapeutics, naked or conjugated, are
evaluated for cross-reactivity with normal tissues,
immunogenicity/antibody production, conjugate or linker toxicity
and "bystander" toxicity of radiolabelled species. Nonetheless,
such studies may have to be individualized to address specific
concerns and following the guidance set by ICH S6 (Safety studies
for biotechnological products, also noted above). As such, the
general principles are that the products are sufficiently well
characterized, impurities/contaminants have been removed, that the
test material is comparable throughout development, that GLP
compliance is maintained.
[0241] The pharmacokinetics (PK) of the immunoglobulins disclosed
herein may be studied in a variety of animal systems, with the most
relevant being non-human primates such as the cynomolgus and rhesus
monkeys. Single or repeated i.v./s.c. administrations over a dose
range of 6000-fold (0.05-300 mg/kg) can be evaluated for half-life
(days to weeks) using plasma concentration and clearance. Volume of
distribution at a steady state and level of systemic absorbance can
also be measured. Examples of such parameters of measurement
generally include maximum observed plasma concentration (Cmax), the
time to reach Cmax (Tmax), the area under the plasma
concentration-time curve from time 0 to infinity [AUC(0-inf] and
apparent elimination half-life (T1/2). Additional measured
parameters could include compartmental analysis of
concentration-time data obtained following i.v. administration and
bioavailability. Examples of pharmacological/toxicological studies
using cynomolgus monkeys have been established for Rituxan and
Zevalin in which monoclonal antibodies to CD20 are cross-reactive.
Biodistribution, dosimetry (for radiolabelled antibodies), and PK
studies can also be done in rodent models. Such studies would
evaluate tolerance at all doses administered, toxicity to local
tissues, preferential localization to rodent xenograft animal
models, and depletion of target cells (e.g. CD20 positive
cells).
[0242] The immunoglobulins disclosed herein may confer superior
pharmacokinetics on Fc-containing therapeutics in animal systems or
in humans. For example, increased binding to FcRn may increase the
half-life and exposure of the Fc-containing drug. Alternatively,
decreased binding to FcRn may decrease the half-life and exposure
of the Fc-containing drug in cases where reduced exposure is
favorable such as when such drug has side-effects.
[0243] It is known in the art that the array of Fc receptors is
differentially expressed on various immune cell types, as well as
in different tissues. Differential tissue distribution of Fc
receptors may ultimately have an impact on the pharmacodynamic (PD)
and pharmacokinetic (PK) properties of immunoglobulins disclosed
herein. Because immunoglobulins of the presentation have varying
affinities for the array of Fc receptors, further screening of the
polypeptides for PD and/or PK properties may be extremely useful
for defining the optimal balance of PD, PK, and therapeutic
efficacy conferred by each candidate polypeptide.
[0244] Pharmacodynamic studies may include, but are not limited to,
targeting specific cells or blocking signaling mechanisms,
measuring inhibition of antigen-specific antibodies etc. The
immunoglobulins disclosed herein may target particular effector
cell populations and thereby direct Fc-containing drugs to induce
certain activities to improve potency or to increase penetration
into a particularly favorable physiological compartment. For
example, neutrophil activity and localization can be targeted by an
immunoglobulin that targets Fc.gamma.RIIIb. Such pharmacodynamic
effects may be demonstrated in animal models or in humans.
[0245] Clinical Use
[0246] The immunoglobulins disclosed herein may find use in a wide
range of products. In one embodiment an immunoglobulin disclosed
herein is a therapeutic, a diagnostic, or a research reagent. The
immunoglobulins may find use in a composition that is monoclonal or
polyclonal. The immunoglobulins disclosed herein may be used for
therapeutic purposes. As will be appreciated by those in the art,
the immunoglobulins disclosed herein may be used for any
therapeutic purpose that antibodies, and the like may be used for.
The immunoglobulins may be administered to a patient to treat
disorders including but not limited to autoimmune and inflammatory
diseases, infectious diseases, and cancer.
[0247] A "patient" for the purposes disclosed herein includes both
humans and other animals, e.g., other mammals. Thus the
immunoglobulins disclosed herein have both human therapy and
veterinary applications. The term "treatment" or "treating" as
disclosed herein is meant to include therapeutic treatment, as well
as prophylactic, or suppressive measures for a disease or disorder.
Thus, for example, successful administration of an immunoglobulin
prior to onset of the disease results in treatment of the disease.
As another example, successful administration of an optimized
immunoglobulin after clinical manifestation of the disease to
combat the symptoms of the disease comprises treatment of the
disease. "Treatment" and "treating" also encompasses administration
of an optimized immunoglobulin after the appearance of the disease
in order to eradicate the disease. Successful administration of an
agent after onset and after clinical symptoms have developed, with
possible abatement of clinical symptoms and perhaps amelioration of
the disease, comprises treatment of the disease. Those "in need of
treatment" include mammals already having the disease or disorder,
as well as those prone to having the disease or disorder, including
those in which the disease or disorder is to be prevented.
[0248] In one embodiment, an immunoglobulin disclosed herein is
administered to a patient having a disease involving inappropriate
expression of a protein or other molecule. Within the scope
disclosed herein this is meant to include diseases and disorders
characterized by aberrant proteins, due for example to alterations
in the amount of a protein present, protein localization,
posttranslational modification, conformational state, the presence
of a mutant or pathogen protein, etc. Similarly, the disease or
disorder may be characterized by alterations molecules including
but not limited to polysaccharides and gangliosides. An
overabundance may be due to any cause, including but not limited to
overexpression at the molecular level, prolonged or accumulated
appearance at the site of action, or increased activity of a
protein relative to normal. Included within this definition are
diseases and disorders characterized by a reduction of a protein.
This reduction may be due to any cause, including but not limited
to reduced expression at the molecular level, shortened or reduced
appearance at the site of action, mutant forms of a protein, or
decreased activity of a protein relative to normal. Such an
overabundance or reduction of a protein can be measured relative to
normal expression, appearance, or activity of a protein, and said
measurement may play an important role in the development and/or
clinical testing of the immunoglobulins disclosed herein.
[0249] By "cancer" and "cancerous" herein refer to or describe the
physiological condition in mammals that is typically characterized
by unregulated cell growth. Examples of cancer include but are not
limited to carcinoma, lymphoma, blastoma, sarcoma (including
liposarcoma), neuroendocrine tumors, mesothelioma, schwanoma,
meningioma, adenocarcinoma, melanoma, and leukemia or lymphoid
malignancies.
[0250] More particular examples of such cancers include hematologic
malignancies, such as Hodgkin's lymphoma; non-Hodgkin's lymphomas
(Burkitt's lymphoma, small lymphocytic lymphoma/chronic lymphocytic
leukemia, mycosis fungoides, mantle cell lymphoma, follicular
lymphoma, diffuse large B-cell lymphoma, marginal zone lymphoma,
hairy cell leukemia and lymphoplasmacytic leukemia), tumors of
lymphocyte precursor cells, including B-cell acute lymphoblastic
leukemia/lymphoma, and T-cell acute lymphoblastic
leukemia/lymphoma, thymoma, tumors of the mature T and NK cells,
including peripheral T-cell leukemias, adult T-cell leukemia/T-cell
lymphomas and large granular lymphocytic leukemia, Langerhans cell
histocytosis, myeloid neoplasias such as acute myelogenous
leukemias, including AML with maturation, AML without
differentiation, acute promyelocytic leukemia, acute myelomonocytic
leukemia, and acute monocytic leukemias, myelodysplastic syndromes,
and chronic myeloproliferative disorders, including chronic
myelogenous leukemia; tumors of the central nervous system such as
glioma, glioblastoma, neuroblastoma, astrocytoma, medulloblastoma,
ependymoma, and retinoblastoma; solid tumors of the head and neck
(eg. nasopharyngeal cancer, salivary gland carcinoma, and
esophagael cancer), lung (eg. small-cell lung cancer, non-small
cell lung cancer, adenocarcinoma of the lung and squamous carcinoma
of the lung), digestive system (eg. gastric or stomach cancer
including gastrointestinal cancer, cancer of the bile duct or
biliary tract, colon cancer, rectal cancer, colorectal cancer, and
anal carcinoma), reproductive system (eg. testicular, penile, or
prostate cancer, uterine, vaginal, vulval, cervical, ovarian, and
endometrial cancer), skin (eg. melanoma, basal cell carcinoma,
squamous cell cancer, actinic keratosis), liver (eg. liver cancer,
hepatic carcinoma, hepatocellular cancer, and hepatoma), bone (eg.
osteoclastoma, and osteolytic bone cancers) additional tissues and
organs (eg. pancreatic cancer, bladder cancer, kidney or renal
cancer, thyroid cancer, breast cancer, cancer of the peritoneum,
and Kaposi's sarcoma), and tumors of the vascular system (eg.
angiosarcoma and hemagiopericytoma).
[0251] By "autoimmune diseases" herein include allogenic islet
graft rejection, alopecia areata, ankylosing spondylitis,
antiphospholipid syndrome, autoimmune Addison's disease,
antineutrophil cytoplasmic autoantibodies (ANCA), autoimmune
diseases of the adrenal gland, autoimmune hemolytic anemia,
autoimmune hepatitis, autoimmune myocarditis, autoimmune
neutropenia, autoimmune oophoritis and orchitis, autoimmune
thrombocytopenia, autoimmune urticaria, Behcet's disease, bullous
pemphigoid, cardiomyopathy, Castleman's syndrome, celiac
spruce-dermatitis, chronic fatigue immune disfunction syndrome,
chronic inflammatory demyelinating polyneuropathy, Churg-Strauss
syndrome, cicatrical pemphigoid, CREST syndrome, cold agglutinin
disease, Crohn's disease, dermatomyositis, discoid lupus, essential
mixed cryoglobulinemia, factor VIII deficiency,
fibromyalgia-fibromyositis, glomerulonephritis, Grave's disease,
Guillain-Barre, Goodpasture's syndrome, graft-versus-host disease
(GVHD), Hashimoto's thyroiditis, hemophilia A, idiopathic pulmonary
fibrosis, idiopathic thrombocytopenia purpura (ITP), IgA
neuropathy, IgM polyneuropathies, immune mediated thrombocytopenia,
juvenile arthritis, Kawasaki's disease, lichen plantus, lupus
erthematosis, Meniere's disease, mixed connective tissue disease,
multiple sclerosis, type 1 diabetes mellitus, myasthenia gravis,
pemphigus vulgaris, pernicious anemia, polyarteritis nodosa,
polychrondritis, polyglandular syndromes, polymyalgia rheumatica,
polymyositis and dermatomyositis, primary agammaglobinulinemia,
primary biliary cirrhosis, psoriasis, psoriatic arthritis,
Reynauld's phenomenon, Reiter's syndrome, rheumatoid arthritis,
sarcoidosis, scleroderma, Sjorgen's syndrome, solid organ
transplant rejection, stiff-man syndrome, systemic lupus
erythematosus, takayasu arteritis, temporal arteristis/giant cell
arteritis, thrombotic thrombocytopenia purpura, ulcerative colitis,
uveitis, vasculitides such as dermatitis herpetiformis vasculitis,
vitiligo, and Wegner's granulomatosis.
[0252] By "inflammatory disorders" herein include acute respiratory
distress syndrome (ARDS), acute septic arthritis, adjuvant
arthritis (Prakken et al., Springer Semin Immunopathol., 2003
August; 25(1):47-63, incorporated entirely by reference), juvenile
idiopathic arthritis (de Kleer et al., Arthritis Rheum. 2003 July;
47(7):2001-10, incorporated entirely by reference), allergic
encephalomyelitis, allergic rhinitis, allergic vasculitis, allergy,
asthma, atherosclerosis, chronic inflammation due to chronic
bacterial or viral infectionis, chronic obstructive pulmonary
disease (COPD), coronary artery disease, encephalitis, inflammatory
bowel disease, inflammatory osteolysis, inflammation associated
with acute and delayed hypersensitivity reactions, inflammation
associated with tumors, peripheral nerve injury or demyelinating
diseases, inflammation associated with tissue trauma such as burns
and ischemia, inflammation due to meningitis, multiple organ injury
syndrome, pulmonary fibrosis, sepsis and septic shock,
Stevens-Johnson syndrome, undifferentiated arthropy, and
undifferentiated spondyloarth ropathy.
[0253] Some autoimmune and inflammatory diseases that may be
targeted by the immunoglobulins disclosed herein include Systemic
Lupus Erythematosus, Rheumatoid arthritis, Sjogren's syndrome,
Multiple sclerosis, Idiopathic thrombocytopenic purpura (ITP),
Graves disease, Inflammatory bowel disease, Psoriasis, Type I
diabetes, and Asthma.
[0254] Immunoglobulins disclosed herein may be utilized to modulate
the activity of the immune system, and in some cases to mimic the
effects of IVIg therapy in a more controlled, specific, and
efficient manner. Thus immunoglobulins disclosed herein may be used
as immunomodulatory therapeutics. IVIg is effectively a high dose
of immunoglobulins delivered intravenously. In general, IVIg has
been used to downregulate autoimmune conditions. It has been
hypothesized that the therapeutic mechanism of action of IVIg
involves ligation of Fc receptors at high frequency (J. Bayry et
al., 2003, Transfusion Clinique et Biologique 10: 165-169; Binstadt
et al., 2003, J Allergy Clin. Immunol, 697-704). Indeed animal
models of Ithrombocytopenia purpura (ITP) show that the isolated Fc
are the active portion of IVIg (Samuelsson et al, 2001, Pediatric
Research 50(5), 551). For use in therapy, immunoglobulin's are
harvested from thousands of donors, with all of the concomitant
problems associated with non-recombinant biotherapeutics collected
from humans. An immunoglobulin disclosed herein should serve all of
the roles of IVIg while being manufactured as a recombinant protein
rather than harvested from donors.
[0255] The immunomodulatory effects of IVIg may be dependent on
productive interaction with one or more Fc ligands, including but
not limited to Fc.gamma.Rs, complement proteins, and FcRn. In some
embodiments, immunoglobulins disclosed herein may be used to
promote anti-inflammatory activity (Samuelsson et al., 2001,
Science 291: 484-486) and or to reduce autoimmunity (Hogarth, 2002,
Current Opinion in Immunology, 14:798-802). In one embodiment, Fc
variants that provide enhanced binding to the inhibitory receptor
Fc.gamma.RIIb provide an enhancement to the IVIg therapeutic
approach. Such Fc variants would thus function as Fc.gamma.RIIb
agonists, and would be expected to enhance the beneficial effects
of IVIg as an autoimmune disease therapeutic and also as a
modulator of B-cell proliferation. In addition, such
Fc.gamma.RIIb-enhanced Fc variants may also be further modified to
have the same or limited binding to other receptors. In additional
embodiments, the Fc variants with enhanced Fc.gamma.RIIb affinity
may be combined with mutations that reduce or ablate to other
receptors, thereby potentially further minimizing side effects
during therapeutic use.
[0256] Binding to or blocking Fc receptors on immune system cells
may be used to influence immune response in immunological
conditions including but not limited to idiopathic thrombocytopenia
purpura (ITP) and rheumatoid arthritis (RA) among others. By use of
the affinity enhanced Fc variants disclosed herein, the dosages
required in typical IVIg applications may be reduced while
obtaining a substantially similar therapeutic effect. Binding
enhancements to Fc.gamma.RIIb would increase expression or
inhibitory activity, as needed, of that receptor and improve
efficacy. In addition, modulated affinity of the Fc variants for
activating Fc.gamma.Rs, FcRn, and/or also complement may also
provide benefits.
[0257] Such immunomodulatory applications of the immunoglobulins
disclosed herein may also be utilized in the treatment of
oncological indications, especially those for which therapy
involves antibody-dependant cytotoxic mechanisms. For example, an
Fc variant that enhances affinity to Fc.gamma.RIIb may be used to
antagonize this inhibitory receptor, for example by binding to the
Fc/Fc.gamma.RIIb binding site but failing to trigger, or reducing
cell signaling, potentially enhancing the effect of antibody-based
anti-cancer therapy. Such Fc variants, functioning as Fc.gamma.RIIb
antagonists, may either block the inhibitory properties of
Fc.gamma.RIIb, or induce its inhibitory function as in the case of
IVIg. An Fc.gamma.RIIb antagonist may be used as co-therapy in
combination with any other therapeutic, including but not limited
to antibodies, acting on the basis of ADCC related cytotoxicity.
Fc.gamma.RIIb antagonistic Fc variants of this type may be isolated
Fc or Fc fragments, although in alternate embodiments
immunoglobulins may be used.
[0258] By "infectious diseases" herein include diseases caused by
pathogens such as viruses, bacteria, fungi, protozoa, and
parasites. Infectious diseases may be caused by viruses including
adenovirus, cytomegalovirus, dengue, Epstein-Barr, hanta, hepatitis
A, hepatitis B, hepatitis C, herpes simplex type I, herpes simplex
type II, human immunodeficiency virus, (HIV), human papilloma virus
(HPV), influenza, measles, mumps, papova virus, polio, respiratory
syncytial virus, rinderpest, rhinovirus, rotavirus, rubella, SARS
virus, smallpox, viral meningitis, and the like. Infections
diseases may also be caused by bacteria including Bacillus
antracis, Borrelia burgdorferi, Campylobacter jejuni, Chlamydia
trachomatis, Clostridium botulinum, Clostridium tetani, Diptheria,
E. coli, Legionella, Helicobacter pylori, Mycobacterium rickettsia,
Mycoplasma nesisseria, Pertussis, Pseudomonas aeruginosa, S.
pneumonia, Streptococcus, Staphylococcus, Vibria cholerae, Yersinia
pestis, and the like. Infectious diseases may also be caused by
fungi such as Aspergillus fumigatus, Blastomyces dermatitidis,
Candida albicans, Coccidioides immitis, Cryptococcus neoformans,
Histoplasma capsulatum, Penicillium marneffei, and the like.
Infectious diseases may also be caused by protozoa and parasites
such as chlamydia, kokzidioa, leishmania, malaria, rickettsia,
trypanosoma, and the like.
[0259] Furthermore, antibodies disclosed herein may be used to
prevent or treat additional conditions including but not limited to
heart conditions such as congestive heart failure (CHF),
myocarditis and other conditions of the myocardium; skin conditions
such as rosecea, acne, and eczema; bone and tooth conditions such
as bone loss, osteoporosis, Paget's disease, Langerhans' cell
histiocytosis, periodontal disease, disuse osteopenia,
osteomalacia, monostotic fibrous dysplasia, polyostotic fibrous
dysplasia, bone metastasis, bone pain management, humoral malignant
hypercalcemia, periodontal reconstruction, spinal cord injury, and
bone fractures; metabolic conditions such as Gaucher's disease;
endocrine conditions such as Cushing's syndrome; and neurological
conditions.
[0260] A number of the receptors that may interact with the
immunoglobulins disclosed herein are polymorphic in the human
population. For a given patient or population of patients, the
efficacy of the immunoglobulins disclosed herein may be affected by
the presence or absence of specific polymorphisms in proteins. For
example, Fc.gamma.RIIIa is polymorphic at position 158, which is
commonly either V (high affinity) or F (low affinity). Patients
with the V/V homozygous genotype are observed to have a better
clinical response to treatment with the anti-CD20 antibody
Rituxan.RTM. (rituximab), likely because these patients mount a
stronger NK response (Dall'Ozzo et. al. (2004) Cancer Res.
64:4664-9, incorporated entirely by reference). Additional
polymorphisms include but are not limited to Fc.gamma.RIIa R131 or
H131, and such polymorphisms are known to either increase or
decrease Fc binding and subsequent biological activity, depending
on the polymorphism. immunoglobulins disclosed herein may bind
preferentially to a particular polymorphic form of a receptor, for
example Fc.gamma.RIIIa 158 V, or to bind with equivalent affinity
to all of the polymorphisms at a particular position in the
receptor, for example both the 158V and 158F polymorphisms of
Fc.gamma.RIIIa. In one embodiment, immunoglobulins disclosed herein
may have equivalent binding to polymorphisms may be used in an
antibody to eliminate the differential efficacy seen in patients
with different polymorphisms. Such a property may give greater
consistency in therapeutic response and reduce non-responding
patient populations. Such variant Fc with indentical binding to
receptor polymorphisms may have increased biological activity, such
as ADCC, CDC or circulating half-life, or alternatively decreased
activity, via modulation of the binding to the relevant Fc
receptors. In one embodiment, immunoglobulins disclosed herein may
bind with higher or lower affinity to one of the polymorphisms of a
receptor, either accentuating the existing difference in binding or
reversing the difference. Such a property may allow creation of
therapeutics particularly tailored for efficacy with a patient
population possessing such polymorphism. For example, a patient
population possessing a polymorphism with a higher affinity for an
inhibitory receptor such as Fc.gamma.RIIb could receive a drug
containing an Fc variant with reduced binding to such polymorphic
form of the receptor, creating a more efficacious drug.
[0261] In one embodiment, patients are screened for one or more
polymorphisms in order to predict the efficacy of the
immunoglobulins disclosed herein. This information may be used, for
example, to select patients to include or exclude from clinical
trials or, post-approval, to provide guidance to physicians and
patients regarding appropriate dosages and treatment options. For
example, in patients that are homozygous or heterozygous for
Fc.gamma.RIIIa 158F antibody drugs such as the anti-CD20 mAb,
Rituximab are minimially effective (Carton 2002 Blood 99: 754-758;
Weng 2003 J. Clin. Oncol. 21:3940-3947, both incorporated entirely
by reference); such patients may show a much better clinical
response to the antibodies disclosed herein. In one embodiment,
patients are selected for inclusion in clinical trials for an
immunoglobulin disclosed herein if their genotype indicates that
they are likely to respond significantly better to an
immunoglobulin disclosed herein as compared to one or more
currently used immunoglobulin therapeutics. In another embodiment,
appropriate dosages and treatment regimens are determined using
such genotype information. In another embodiment, patients are
selected for inclusion in a clinical trial or for receipt of
therapy post-approval based on their polymorphism genotype, where
such therapy contains an immunoglobulin engineered to be
specifically efficacious for such population, or alternatively
where such therapy contains an Fc variant that does not show
differential activity to the different forms of the
polymorphism.
[0262] Also disclosed are diagnostic tests to identify patients who
are likely to show a favorable clinical response to an
immunoglobulin disclosed herein, or who are likely to exhibit a
significantly better response when treated with an immunoglobulin
disclosed herein versus one or more currently used immunoglobulin
therapeutics. Any of a number of methods for determining Fc.gamma.R
polymorphisms in humans known in the art may be used.
[0263] Furthermore, also disclosed are prognostic tests performed
on clinical samples such as blood and tissue samples. Such tests
may assay for effector function activity, including but not limited
to ADCC, CDC, phagocytosis, and opsonization, or for killing,
regardless of mechanism, of cancerous or otherwise pathogenic
cells. In one embodiment, ADCC assays, such as those described
previously, are used to predict, for a specific patient, the
efficacy of a given immunoglobulin disclosed herein. Such
information may be used to identify patients for inclusion or
exclusion in clinical trials, or to inform decisions regarding
appropriate dosages and treatment regemins. Such information may
also be used to select a drug that contains a particular
immunoglobulin that shows superior activity in such assay.
[0264] Formulation
[0265] Pharmaceutical compositions are contemplated wherein an
immunoglobulin disclosed herein and one or more therapeutically
active agents are formulated. Formulations of the immunoglobulins
disclosed herein are prepared for storage by mixing said
immunoglobulin having the desired degree of purity with optional
pharmaceutically acceptable carriers, excipients or stabilizers
(Remington's Pharmaceutical Sciences 16th edition, Osol, A. Ed.,
1980, incorporated entirely by reference), in the form of
lyophilized formulations or aqueous solutions. Acceptable carriers,
excipients, or stabilizers are nontoxic to recipients at the
dosages and concentrations employed, and include buffers such as
phosphate, citrate, acetate, and other organic acids; antioxidants
including ascorbic acid and methionine; preservatives (such as
octadecyldimethylbenzyl ammonium chloride; hexamethonium chloride;
benzalkonium chloride, benzethonium chloride; phenol, butyl
orbenzyl alcohol; alkyl parabens such as methyl or propyl paraben;
catechol; resorcinol; cyclohexanol; 3-pentanol; and m-cresol); low
molecular weight (less than about 10 residues) polypeptides;
proteins, such as serum albumin, gelatin, or immunoglobulins;
hydrophilic polymers such as polyvinylpyrrolidone; amino acids such
as glycine, glutamine, asparagine, histidine, arginine, or lysine;
monosaccharides, disaccharides, and other carbohydrates including
glucose, mannose, or dextrins; chelating agents such as EDTA;
sugars such as sucrose, mannitol, trehalose or sorbitol; sweeteners
and other flavoring agents; fillers such as microcrystalline
cellulose, lactose, corn and other starches; binding agents;
additives; coloring agents; salt-forming counter-ions such as
sodium; metal complexes (e.g. Zn-protein complexes); and/or
non-ionic surfactants such as TWEEN.TM., PLURONICS.TM. or
polyethylene glycol (PEG). In one embodiment, the pharmaceutical
composition that comprises the immunoglobulin disclosed herein may
be in a water-soluble form, such as being present as
pharmaceutically acceptable salts, which is meant to include both
acid and base addition salts. "Pharmaceutically acceptable acid
addition salt" refers to those salts that retain the biological
effectiveness of the free bases and that are not biologically or
otherwise undesirable, formed with inorganic acids such as
hydrochloric acid, hydrobromic acid, sulfuric acid, nitric acid,
phosphoric acid and the like, and organic acids such as acetic
acid, propionic acid, glycolic acid, pyruvic acid, oxalic acid,
maleic acid, malonic acid, succinic acid, fumaric acid, tartaric
acid, citric acid, benzoic acid, cinnamic acid, mandelic acid,
methanesulfonic acid, ethanesulfonic acid, p-toluenesulfonic acid,
salicylic acid and the like. "Pharmaceutically acceptable base
addition salts" include those derived from inorganic bases such as
sodium, potassium, lithium, ammonium, calcium, magnesium, iron,
zinc, copper, manganese, aluminum salts and the like.
[0266] Some embodiments include at least one of the ammonium,
potassium, sodium, calcium, and magnesium salts. Salts derived from
pharmaceutically acceptable organic non-toxic bases include salts
of primary, secondary, and tertiary amines, substituted amines
including naturally occurring substituted amines, cyclic amines and
basic ion exchange resins, such as isopropylamine, trimethylamine,
diethylamine, triethylamine, tripropylamine, and ethanolamine. The
formulations to be used for in vivo administration may be sterile.
This is readily accomplished by filtration through sterile
filtration membranes or other methods.
[0267] The immunoglobulins disclosed herein may also be formulated
as immunoliposomes. A liposome is a small vesicle comprising
various types of lipids, phospholipids and/or surfactant that is
useful for delivery of a therapeutic agent to a mammal. Liposomes
containing the immunoglobulin are prepared by methods known in the
art, such as described in Epstein et al., 1985, Proc Natl Acad Sci
USA, 82:3688; Hwang et al., 1980, Proc Natl Acad Sci USA, 77:4030;
U.S. Pat. No. 4,485,045; U.S. Pat. No. 4,544,545; and PCT WO
97/38731, all incorporated entirely by reference. Liposomes with
enhanced circulation time are disclosed in U.S. Pat. No. 5,013,556,
incorporated entirely by reference. The components of the liposome
are commonly arranged in a bilayer formation, similar to the lipid
arrangement of biological membranes. Particularly useful liposomes
can be generated by the reverse phase evaporation method with a
lipid composition comprising phosphatidylcholine, cholesterol and
PEG-derivatized phosphatidylethanolamine (PEG-PE). Liposomes are
extruded through filters of defined pore size to yield liposomes
with the desired diameter. A chemotherapeutic agent or other
therapeutically active agent is optionally contained within the
liposome (Gabizon et al., 1989, J National Cancer Inst 81:1484,
incorporated entirely by reference).
[0268] The immunoglobulin and other therapeutically active agents
may also be entrapped in microcapsules prepared by methods
including but not limited to coacervation techniques, interfacial
polymerization (for example using hydroxymethylcellulose or
gelatin-microcapsules, or poly-(methylmethacylate) microcapsules),
colloidal drug delivery systems (for example, liposomes, albumin
microspheres, microemulsions, nano-particles and nanocapsules), and
macroemulsions. Such techniques are disclosed in Remington's
Pharmaceutical Sciences 16th edition, Osol, A. Ed., 1980,
incorporated entirely by reference. Sustained-release preparations
may be prepared. Suitable examples of sustained-release
preparations include semipermeable matrices of solid hydrophobic
polymer, which matrices are in the form of shaped articles, e.g.
films, or microcapsules. Examples of sustained-release matrices
include polyesters, hydrogels (for example
poly(2-hydroxyethyl-methacrylate), or poly(vinylalcohol)),
polylactides (U.S. Pat. No. 3,773,919, incorporated entirely by
reference), copolymers of L-glutamic acid and gamma
ethyl-L-glutamate, non-degradable ethylene-vinyl acetate,
degradable lactic acid-glycolic acid copolymers such as the Lupron
Depot.RTM. (which are injectable microspheres composed of lactic
acid-glycolic acid copolymer and leuprolide acetate),
poly-D-(-)-3-hydroxybutyric acid, and ProLease.RTM. (commercially
available from Alkermes), which is a microsphere-based delivery
system composed of the desired bioactive molecule incorporated into
a matrix of poly-DL-lactide-co-glycolide (PLG).
[0269] Administration
[0270] Administration of the pharmaceutical composition comprising
an immunoglobulin disclosed herein, e.g., in the form of a sterile
aqueous solution, may be done in a variety of ways, including, but
not limited to orally, subcutaneously, intravenously, intranasally,
intraotically, transdermally, topically (e.g., gels, salves,
lotions, creams, etc.), intraperitoneally, intramuscularly,
intrapulmonary, vaginally, parenterally, rectally, or
intraocularly. In some instances, for example for the treatment of
wounds, inflammation, etc., the immunoglobulin may be directly
applied as a solution or spray. As is known in the art, the
pharmaceutical composition may be formulated accordingly depending
upon the manner of introduction.
[0271] Subcutaneous administration may be used in circumstances
where the patient may self-administer the pharmaceutical
composition. Many protein therapeutics are not sufficiently potent
to allow for formulation of a therapeutically effective dose in the
maximum acceptable volume for subcutaneous administration. This
problem may be addressed in part by the use of protein formulations
comprising arginine-HCl, histidine, and polysorbate (see WO
04091658, incorporated entirely by reference). Antibodies disclosed
herein may be more amenable to subcutaneous administration due to,
for example, increased potency, improved serum half-life, or
enhanced solubility.
[0272] As is known in the art, protein therapeutics are often
delivered by IV infusion or bolus. The antibodies disclosed herein
may also be delivered using such methods. For example,
administration may be by intravenous infusion with 0.9% sodium
chloride as an infusion vehicle.
[0273] Pulmonary delivery may be accomplished using an inhaler or
nebulizer and a formulation comprising an aerosolizing agent. For
example, AERx.RTM. inhalable technology commercially available from
Aradigm, or Inhance.TM. pulmonary delivery system commercially
available from Nektar Therapeutics may be used. Antibodies
disclosed herein may be more amenable to intrapulmonary delivery.
FcRn is present in the lung, and may promote transport from the
lung to the bloodstream (e.g. Syntonix WO 04004798, Bitonti et al.
(2004) Proc. Nat. Acad. Sci. 101:9763-8, both incorporated entirely
by reference). Accordingly, antibodies that bind FcRn more
effectively in the lung or that are released more efficiently in
the bloodstream may have improved bioavailability following
intrapulmonary administration. Antibodies disclosed herein may also
be more amenable to intrapulmonary administration due to, for
example, improved solubility or altered isoelectric point.
[0274] Furthermore, immunoglobulins disclosed herein may be more
amenable to oral delivery due to, for example, improved stability
at gastric pH and increased resistance to proteolysis. Furthermore,
FcRn appears to be expressed in the intestinal epithelia of adults
(Dickinson et al. (1999) J. Clin. Invest. 104:903-11, incorporated
entirely by reference), so antibodies disclosed herein with
improved FcRn interaction profiles may show enhanced
bioavailability following oral administration. FcRn mediated
transport of antibodies may also occur at other mucus membranes
such as those in the gastrointestinal, respiratory, and genital
tracts (Yoshida et al. (2004) Immunity 20:769-83, incorporated
entirely by reference).
[0275] In addition, any of a number of delivery systems are known
in the art and may be used to administer the antibodies disclosed
herein. Examples include, but are not limited to, encapsulation in
liposomes, microparticles, microspheres (e.g., PLA/PGA
microspheres), and the like. Alternatively, an implant of a porous,
non-porous, or gelatinous material, including membranes or fibers,
may be used. Sustained release systems may comprise a polymeric
material or matrix such as polyesters, hydrogels,
poly(vinylalcohol),polylactides, copolymers of L-glutamic acid and
ethyl-L-gutamate, ethylene-vinyl acetate, lactic acid-glycolic acid
copolymers such as the Lupron Depot.RTM., and
poly-D-(-)-3-hydroxyburyric acid. It is also possible to administer
a nucleic acid encoding an immunoglobulin disclosed herein, for
example by retroviral infection, direct injection, or coating with
lipids, cell surface receptors, or other transfection agents. In
all cases, controlled release systems may be used to release the
immunoglobulin at or close to the desired location of action.
[0276] Dosing
[0277] The dosing amounts and frequencies of administration are, in
one embodiment, selected to be therapeutically or prophylactically
effective. As is known in the art, adjustments for protein
degradation, systemic versus localized delivery, and rate of new
protease synthesis, as well as the age, body weight, general
health, sex, diet, time of administration, drug interaction and the
severity of the condition may be necessary, and will be
ascertainable with routine experimentation by those skilled in the
art.
[0278] The concentration of the therapeutically active
immunoglobulin in the formulation may vary from about 0.1 to 100
weight %. In one embodiment, the concentration of the
immunoglobulin is in the range of 0.003 to 1.0 molar. In order to
treat a patient, a therapeutically effective dose of the
immunoglobulin disclosed herein may be administered. By
"therapeutically effective dose" herein is meant a dose that
produces the effects for which it is administered. The exact dose
will depend on the purpose of the treatment, and will be
ascertainable by one skilled in the art using known techniques.
Dosages may range from 0.0001 to 100 mg/kg of body weight or
greater, for example 0.1, 1, 10, or 50 mg/kg of body weight. In one
embodiment, dosages range from 1 to 10 mg/kg.
[0279] In some embodiments, only a single dose of the
immunoglobulin is used. In other embodiments, multiple doses of the
immunoglobulin are administered. The elapsed time between
administrations may be less than 1 hour, about 1 hour, about 1-2
hours, about 2-3 hours, about 3-4 hours, about 6 hours, about 12
hours, about 24 hours, about 48 hours, about 2-4 days, about 4-6
days, about 1 week, about 2 weeks, or more than 2 weeks.
[0280] In other embodiments the antibodies disclosed herein are
administered in metronomic dosing regimes, either by continuous
infusion or frequent administration without extended rest periods.
Such metronomic administration may involve dosing at constant
intervals without rest periods. Typically such regimens encompass
chronic low-dose or continuous infusion for an extended period of
time, for example 1-2 days, 1-2 weeks, 1-2 months, or up to 6
months or more. The use of lower doses may minimize side effects
and the need for rest periods.
[0281] In certain embodiments the immunoglobulin disclosed herein
and one or more other prophylactic or therapeutic agents are
cyclically administered to the patient. Cycling therapy involves
administration of a first agent at one time, a second agent at a
second time, optionally additional agents at additional times,
optionally a rest period, and then repeating this sequence of
administration one or more times. The number of cycles is typically
from 2-10. Cycling therapy may reduce the development of resistance
to one or more agents, may minimize side effects, or may improve
treatment efficacy.
[0282] Combination Therapies
[0283] The antibodies disclosed herein may be administered
concomitantly with one or more other therapeutic regimens or
agents. The additional therapeutic regimes or agents may be used to
improve the efficacy or safety of the immunoglobulin. Also, the
additional therapeutic regimes or agents may be used to treat the
same disease or a comorbidity rather than to alter the action of
the immunoglobulin. For example, an immunoglobulin disclosed herein
may be administered to the patient along with chemotherapy,
radiation therapy, or both chemotherapy and radiation therapy. The
immunoglobulin disclosed herein may be administered in combination
with one or more other prophylactic or therapeutic agents,
including but not limited to cytotoxic agents, chemotherapeutic
agents, cytokines, growth inhibitory agents, anti-hormonal agents,
kinase inhibitors, anti-angiogenic agents, cardioprotectants,
immunostimulatory agents, immunosuppressive agents, agents that
promote proliferation of hematological cells, angiogenesis
inhibitors, protein tyrosine kinase (PTK) inhibitors, additional
antibodies, Fc.gamma.RIIb or other Fc receptor inhibitors, or other
therapeutic agents.
[0284] The terms "in combination with" and "co-administration" are
not limited to the administration of said prophylactic or
therapeutic agents at exactly the same time. Instead, it is meant
that the immunoglobulin disclosed herein and the other agent or
agents are administered in a sequence and within a time interval
such that they may act together to provide a benefit that is
increased versus treatment with only either the immunoglobulin
disclosed herein or the other agent or agents. In some embodiments,
immunoglobulins disclosed herein and the other agent or agents act
additively, and sometimes synergistically. Such molecules are
suitably present in combination in amounts that are effective for
the purpose intended. The skilled medical practitioner can
determine empirically, or by considering the pharmacokinetics and
modes of action of the agents, the appropriate dose or doses of
each therapeutic agent, as well as the appropriate timings and
methods of administration.
[0285] In one embodiment, the antibodies disclosed herein are
administered with one or more additional molecules comprising
antibodies or Fc. The antibodies disclosed herein may be
co-administered with one or more other antibodies that have
efficacy in treating the same disease or an additional comorbidity;
for example two antibodies may be administered that recognize two
antigens that are overexpressed in a given type of cancer, or two
antigens that mediate pathogenesis of an autoimmune or infectious
disease.
[0286] Examples of anti-cancer antibodies that may be
co-administered include, but are not limited to, anti-17-1A cell
surface antigen antibodies such as Panorex.TM. (edrecolomab);
anti-4-1 BB antibodies; anti-4Dc antibodies; anti-A33 antibodies
such as A33 and CDP-833; anti-.alpha.4.beta.1 integrin antibodies
such as natalizumab; anti-.alpha.4.beta.7 integrin antibodies such
as LDP-02; anti-.alpha.V.beta.1 integrin antibodies such as F-200,
M-200, and SJ-749; anti-.alpha.V.beta.3 integrin antibodies such as
abciximab, CNTO-95, Mab-17E6, and Vitaxin.TM.; anti-complement
factor 5 (C5) antibodies such as 5G1.1; anti-CA125 antibodies such
as OvaRex.RTM. (oregovomab); anti-CD3 antibodies such as
Nuvion.RTM. (visilizumab) and Rexomab; anti-CD4 antibodies such as
IDEC-151, MDX-CD4, OKT4A; anti-CD6 antibodies such as Oncolysin B
and Oncolysin CD6; anti-CD7 antibodies such as HB2; anti-CD19
antibodies such as B43, MT-103, and Oncolysin B; anti-CD20
antibodies such as 2H7, 2H7.v16, 2H7.v114, 2H7.v115, Bexxar.RTM.
(tositumomab, 1-131 labeled anti-CD20), Rituxan.RTM. (rituximab),
and Zevalin.RTM. (Ibritumomab tiuxetan, Y-90 labeled anti-CD20);
anti-CD22 antibodies such as Lymphocide.TM. (epratuzumab, Y-90
labeled anti-CD22); anti-CD23 antibodies such as IDEC-152;
anti-CD25 antibodies such as basiliximab and Zenapax.RTM.
(daclizumab); anti-CD30 antibodies such as AC10, MDX-060, and
SGN-30; anti-CD33 antibodies such as Mylotarg.RTM. (gemtuzumab
ozogamicin), Oncolysin M, and Smart M195; anti-CD38 antibodies;
anti-CD40 antibodies such as SGN-40 and toralizumab; anti-CD40L
antibodies such as 5c8, Antova.TM., and IDEC-131; anti-CD44
antibodies such as bivatuzumab; anti-CD46 antibodies; anti-CD52
antibodies such as Campath.RTM. (alemtuzumab); anti-CD55 antibodies
such as SC-1; anti-CD56 antibodies such as huN901-DM1; anti-CD64
antibodies such as MDX-33; anti-CD66e antibodies such as XR-303;
anti-CD74 antibodies such as IMMU-110; anti-CD80 antibodies such as
galiximab and IDEC-114; anti-CD89 antibodies such as MDX-214;
anti-CD123 antibodies; anti-CD138 antibodies such as B-B4-DM1;
anti-CD146 antibodies such as AA-98; anti-CD148 antibodies;
anti-CEA antibodies such as cT84.66, labetuzumab, and Pentacea.TM.;
anti-CTLA-4 antibodies such as MDX-101; anti-CXCR4 antibodies;
anti-EGFR antibodies such as ABX-EGF, Erbitux.RTM. (cetuximab),
IMC-C225, and Merck Mab 425; anti-EpCAM antibodies such as
Crucell's anti-EpCAM, ING-1, and IS-IL-2; anti-ephrin B2/EphB4
antibodies; anti-Her2 antibodies such as Herceptin.RTM., MDX-210;
anti-FAP (fibroblast activation protein) antibodies such as
sibrotuzumab; anti-ferritin antibodies such as NXT-211; anti-FGF-1
antibodies; anti-FGF-3 antibodies; anti-FGF-8 antibodies; anti-FGFR
antibodies, anti-fibrin antibodies; anti-G250 antibodies such as
WX-G250 and Rencarex.RTM.; anti-GD2 ganglioside antibodies such as
EMD-273063 and TriGem; anti-GD3 ganglioside antibodies such as
BEC2, KW-2871, and mitumomab; anti-gpIIb/IIIa antibodies such as
ReoPro; anti-heparinase antibodies; anti-Her2/ErbB2 antibodies such
as Herceptin.RTM. (trastuzumab), MDX-210, and pertuzumab; anti-HLA
antibodies such as Oncolym.RTM., Smart 1D10; anti-HM1.24
antibodies; anti-ICAM antibodies such as ICM3; anti-IgA receptor
antibodies; anti-IGF-1 antibodies such as CP-751871 and EM-164;
anti-IGF-1R antibodies such as IMC-A12; anti-IL-6 antibodies such
as CNTO-328 and elsilimomab; anti-IL-15 antibodies such as
HuMax.TM.-IL15; anti-KDR antibodies; anti-laminin 5 antibodies;
anti-Lewis Y antigen antibodies such as Hu3S193 and IGN-311;
anti-MCAM antibodies; anti-Muc1 antibodies such as BravaRex and
TriAb; anti-NCAM antibodies such as ERIC-1 and ICRT; anti-PEM
antigen antibodies such as Theragyn and Therex; anti-PSA
antibodies; anti-PSCA antibodies such as IG8; anti-Ptk antibodies;
anti-PTN antibodies; anti-RANKL antibodies such as AMG-162;
anti-RLIP76 antibodies; anti-SK-1 antigen antibodies such as
Monopharm C; anti-STEAP antibodies; anti-TAG72 antibodies such as
CC49-SCA and MDX-220; anti-TGF-.beta. antibodies such as CAT-152;
anti-TNF-.alpha. antibodies such as CDP571, CDP870, D2E7,
Humira.RTM. (adalimumab), and Remicade.RTM. (infliximab);
anti-TRAIL-R1 and TRAIL-R2 antibodies; anti-VE-cadherin-2
antibodies; and anti-VLA-4 antibodies such as Antegren.TM..
Furthermore, anti-idiotype antibodies including but not limited to
the GD3 epitope antibody BEC2 and the gp72 epitope antibody 105AD7,
may be used. In addition, bispecific antibodies including but not
limited to the anti-CD3/CD20 antibody Bi20 may be used.
[0287] Examples of antibodies that may be co-administered to treat
autoimmune or inflammatory disease, transplant rejection, GVHD, and
the like include, but are not limited to, anti-.alpha.4.beta.7
integrin antibodies such as LDP-02, anti-beta2 integrin antibodies
such as LDP-01, anti-complement (C5) antibodies such as 5G1.1,
anti-CD2 antibodies such as BTI-322, MEDI-507, anti-CD3 antibodies
such as OKT3, SMART anti-CD3, anti-CD4 antibodies such as IDEC-151,
MDX-CD4, OKT4A, anti-CD11 a antibodies, anti-CD14 antibodies such
as IC14, anti-CD18 antibodies, anti-CD23 antibodies such as IDEC
152, anti-CD25 antibodies such as Zenapax, anti-CD40L antibodies
such as 5c8, Antova, IDEC-131, anti-CD64 antibodies such as MDX-33,
anti-CD80 antibodies such as IDEC-114, anti-CD147 antibodies such
as ABX-CBL, anti-E-selectin antibodies such as CDP850,
anti-gpIIb/IIIa antibodies such as ReoPro/Abcixima, anti-ICAM-3
antibodies such as ICM3, anti-ICE antibodies such as VX-740,
anti-Fc.gamma.R1 antibodies such as MDX-33, anti-IgE antibodies
such as rhuMab-E25, anti-IL-4 antibodies such as SB-240683,
anti-IL-5 antibodies such as SB-240563, SCH55700, anti-IL-8
antibodies such as ABX-IL8, anti-interferon gamma antibodies, and
anti-TNFa antibodies such as CDP571, CDP870, D2E7, Infliximab,
MAK-195F, anti-VLA-4 antibodies such as Antegren. Examples of other
Fc-containing molecules that may be co-administered to treat
autoimmune or inflammatory disease, transplant rejection, GVHD, and
the like include, but are not limited to, the p75 TNF receptor/Fc
fusion Enbrel.RTM. (etanercept) and Regeneron's IL-1 trap.
[0288] Examples of antibodies that may be co-administered to treat
infectious diseases include, but are not limited to, anti-anthrax
antibodies such as ABthrax, anti-CMV antibodies such as CytoGam and
sevirumab, anti-cryptosporidium antibodies such as CryptoGAM,
Sporidin-G, anti-helicobacter antibodies such as Pyloran,
anti-hepatitis B antibodies such as HepeX-B, Nabi-HB, anti-HIV
antibodies such as HRG-214, anti-RSV antibodies such as felvizumab,
HNK-20, palivizumab, RespiGam, and anti-staphylococcus antibodies
such as Aurexis, Aurograb, BSYX-A110, and SE-Mab.
[0289] Alternatively, the antibodies disclosed herein may be
co-administered or with one or more other molecules that compete
for binding to one or more Fc receptors. For example,
co-administering inhibitors of the inhibitory receptor
Fc.gamma.RIIb may result in increased effector function. Similarly,
co-administering inhibitors of the activating receptors such as
Fc.gamma.RIIa may minimize unwanted effector function. Fc receptor
inhibitors include, but are not limited to, Fc molecules that are
engineered to act as competitive inhibitors for binding to
Fc.gamma.RIIb Fc.gamma.RIIa, or other Fc receptors, as well as
other immunoglobulins and specifically the treatment called IVIg
(intravenous immunoglobulin). In one embodiment, the inhibitor is
administered and allowed to act before the immunoglobulin is
administered. An alternative way of achieving the effect of
sequential dosing would be to provide an immediate release dosage
form of the Fc receptor inhibitor and then a sustained release
formulation of an immunoglobulin disclosed herein. The immediate
release and controlled release formulations could be administered
separately or be combined into one unit dosage form. Administration
of an Fc.gamma.RIIb inhibitor may also be used to limit unwanted
immune responses, for example anti-Factor VIII antibody response
following Factor VIII administration to hemophiliacs.
[0290] In one embodiment, the antibodies disclosed herein are
administered with a chemotherapeutic agent. By "chemotherapeutic
agent" as used herein is meant a chemical compound useful in the
treatment of cancer. Examples of chemotherapeutic agents include
but are not limited to alkylating agents such as thiotepa and
cyclosphosphamide (CYTOXAN.TM.); alkyl sulfonates such as busulfan,
improsulfan and piposulfan; androgens such as calusterone,
dromostanolone propionate, epitiostanol, mepitiostane,
testolactone; anti-adrenals such as aminoglutethimide, mitotane,
trilostane; anti-androgens such as flutamide, nilutamide,
bicalutamide, leuprolide, and goserelin; antibiotics such as
aclacinomysins, actinomycin, authramycin, azaserine, bleomycins,
cactinomycin, calicheamicin, carabicin, caminomycin, carzinophilin,
chromomycins, dactinomycin, daunorubicin, detorubicin,
6-diazo-5-oxo-L-norleucine, doxorubicin, epirubicin, esorubicin,
idarubicin, marcellomycin, mitomycins, mycophenolic acid,
nogalamycin, olivomycins, peplomycin, potfiromycin, puromycin,
quelamycin, rodorubicin, streptonigrin, streptozocin, tubercidin,
ubenimex, zinostatin, zorubicin; anti estrogens including for
example tamoxifen, raloxifene, aromatase inhibiting
4(5)-imidazoles, 4-hydroxytamoxifen, trioxifene, keoxifene, LY
117018, onapristone, and toremifene (Fareston); anti-metabolites
such as methotrexate and 5-fluorouracil (5-FU); folic acid
analogues such as denopterin, methotrexate, pteropterin,
trimetrexate; aziridines such as benzodopa, carboquone, meturedopa,
and uredopa; ethylenimines and methylamelamines including
altretamine, triethylenemelamine, trietylenephosphoramide,
triethylenethiophosphaoramide and trimethylolomelamine; folic acid
replenisher such as frolinic acid; nitrogen mustards such as
chlorambucil, chlornaphazine, cholophosphamide, estramustine,
ifosfamide, mechlorethamine, mechlorethamine oxide hydrochloride,
melphalan, novembichin, phenesterine, prednimustine, trofosfamide,
uracil mustard; nitrosureas such as carmustine, chlorozotocin,
fotemustine, lomustine, nimustine, ranimustine; platinum analogs
such as cisplatin and carboplatin; vinblastine; platinum; proteins
such as arginine deiminase and asparaginase; purine analogs such as
fludarabine, 6-mercaptopurine, thiamiprine, thioguanine; pyrimidine
analogs such as ancitabine, azacitidine, 6-azauridine, carmofur,
cytarabine, dideoxyuridine, doxifluridine, enocitabine,
floxuridine, 5-FU; taxanes, e.g. paclitaxel (TAXOL.RTM.,
Bristol-Myers Squibb Oncology, Princeton, N.J.) and docetaxel
(TAXOTERE.RTM., Rhne-Poulenc Rorer, Antony, France); topoisomerase
inhibitor RFS 2000; thymidylate synthase inhibitor (such as
Tomudex); additional chemotherapeutics including aceglatone;
aldophosphamide glycoside; aminolevulinic acid; amsacrine;
bestrabucil; bisantrene; edatraxate; defofamine; demecolcine;
diaziquone; difluoromethylornithine (DMFO); elformithine;
elliptinium acetate; etoglucid; gallium nitrate; hydroxyurea;
lentinan; lonidamine; mitoguazone; mitoxantrone; mopidamol;
nitracrine; pentostatin; phenamet; pirarubicin; podophyllinic acid;
2-ethylhydrazide; procarbazine; PSK.RTM.; razoxane; sizofuran;
spirogermanium; tenuazonic acid; triaziquone; 2,
2',2''-trichlorotriethylamine; urethan; vindesine; dacarbazine;
mannomustine; mitobronitol; mitolactol; pipobroman; gacytosine;
arabinoside ("Ara-C"); cyclophosphamide; thiotepa; chlorambucil;
gemcitabine; 6-thioguanine; mercaptopurine; methotrexate; etoposide
(VP-16); ifosfamide; mitomycin C; mitoxantrone; vincristine;
vinorelbine; navelbine; novantrone; teniposide; daunomycin;
aminopterin; xeloda; ibandronate; CPT-11; retinoic acid;
esperamicins; capecitabine. Pharmaceutically acceptable salts,
acids or derivatives of any of the above may also be used.
[0291] A chemotherapeutic or other cytotoxic agent may be
administered as a prodrug. By "prodrug" as used herein is meant a
precursor or derivative form of a pharmaceutically active substance
that is less cytotoxic to tumor cells compared to the parent drug
and is capable of being enzymatically activated or converted into
the more active parent form. See, for example Wilman, 1986,
Biochemical Society Transactions, 615th Meeting Belfast,
14:375-382; Stella et al., "Prodrugs: A Chemical Approach to
Targeted Drug Delivery," Directed Drug Delivery; and Borchardt et
al., (ed.): 247-267, Humana Press, 1985, all incorporated entirely
by reference. The prodrugs that may find use with immunoglobulins
disclosed herein include but are not limited to
phosphate-containing prodrugs, thiophosphate-containing prodrugs,
sulfate-containing prodrugs, peptide-containing prodrugs, D-amino
acid-modified prodrugs, glycosylated prodrugs,
beta-lactam-containing prodrugs, optionally substituted
phenoxyacetamide-containing prodrugs or optionally substituted
phenylacetamide-containing prodrugs, 5-fluorocytosine and other
5-fluorouridine prodrugs which can be converted into the more
active cytotoxic free drug. Examples of cytotoxic drugs that can be
derivatized into a prodrug form for use with the antibodies
disclosed herein include but are not limited to any of the
aforementioned chemotherapeutic agents.
[0292] A variety of other therapeutic agents may find use for
administration with the antibodies disclosed herein. In one
embodiment, the immunoglobulin is administered with an
anti-angiogenic agent. By "anti-angiogenic agent" as used herein is
meant a compound that blocks, or interferes to some degree, the
development of blood vessels. The anti-angiogenic factor may, for
instance, be a small molecule or a protein, for example an
antibody, Fc fusion, or cytokine, that binds to a growth factor or
growth factor receptor involved in promoting angiogenesis. In one
embodiment, an anti-angiogenic factor may be an antibody that binds
to Vascular Endothelial Growth Factor (VEGF). Other agents that
inhibit signaling through VEGF may also be used, for example
RNA-based therapeutics that reduce levels of VEGF or VEGF-R
expression, VEGF-toxin fusions, Regeneron's VEGF-trap, and
antibodies that bind VEGF-R. In an alternate embodiment, the
antibody is administered with a therapeutic agent that induces or
enhances adaptive immune response, for example an antibody that
targets CTLA-4. Additional anti-angiogenesis agents include, but
are not limited to, angiostatin (plasminogen fragment),
antithrombin III, angiozyme, ABT-627, Bay 12-9566, benefin,
bevacizumab, bisphosphonates, BMS-275291, cartilage-derived
inhibitor (CDI), CAI, CD59 complement fragment, CEP-7055, Col 3,
combretastatin A-4, endostatin (collagen XVIII fragment), farnesyl
transferase inhibitors, fibronectin fragment, gro-beta,
halofuginone, heparinases, heparin hexasaccharide fragment, HMV833,
human chorionic gonadotropin (hCG), IM-862, interferon alpha,
interferon beta, interferon gamma, interferon inducible protein 10
(IP-10), interleukin-12, kringle 5 (plasminogen fragment),
marimastat, metalloproteinase inhibitors (eg. TIMPs),
2-methodyestradiol, MMI 270 (CGS 27023A), plasminogen activiator
inhibitor (PAl), platelet factor-4 (PF4), prinomastat, prolactin 16
kDa fragment, proliferin-related protein (PRP), PTK 787/ZK 222594,
retinoids, solimastat, squalamine, SS3304, SU5416, SU6668, SU11248,
tetrahydrocortisol-S, tetrathiomolybdate, thalidomide,
thrombospondin-1 (TSP-1), TNP-470, transforming growth factor beta
(TGF-.beta.), vasculostatin, vasostatin (calreticulin fragment),
ZS6126,and ZD6474.
[0293] In one embodiment, the immunoglobulin is administered with a
tyrosine kinase inhibitor. By "tyrosine kinase inhibitor" as used
herein is meant a molecule that inhibits to some extent tyrosine
kinase activity of a tyrosine kinase. Examples of such inhibitors
include but are not limited to quinazolines, such as PD 153035,
4-(3-chloroanilino) quinazoline; pyridopyrimidines;
pyrimidopyrimidines; pyrrolopyrimidines, such as CGP 59326, CGP
60261 and CGP 62706; pyrazolopyrimidines,
4-(phenylamino)-7H-pyrrolo(2,3-d) pyrimidines; curcumin (diferuloyl
methane, 4,5-bis (4-fluoroanilino)phthalimide); tyrphostines
containing nitrothiophene moieties; PD-0183805 (Warner-Lambert);
antisense molecules (e.g. those that bind to ErbB-encoding nucleic
acid); quinoxalines (U.S. Pat. No. 5,804,396); tryphostins (U.S.
Pat. No. 5,804,396); ZD6474 (Astra Zeneca); PTK-787
(Novartis/Schering A G); pan-ErbB inhibitors such as C1-1033
(Pfizer); Affinitac (ISIS 3521; Isis/Lilly); Imatinib mesylate
(ST1571,Gleevec.RTM.; Novartis); PKI 166 (Novartis); GW2016 (Glaxo
SmithKline); C1-1033 (Pfizer); EKB-569 (Wyeth); Semaxinib (Sugen);
ZD6474 (AstraZeneca); PTK-787 (Novartis/Schering AG); INC-1C11
(Imclone); or as described in any of the following patent
publications: U.S. Pat. No. 5,804,396; PCT WO 99/09016 (American
Cyanimid); PCT WO 98/43960 (American Cyanamid); PCT WO 97/38983
(Warner-Lambert); PCT WO 99/06378 (Warner-Lambert); PCT WO 99/06396
(Warner-Lambert); PCT WO 96/30347 (Pfizer, Inc); PCT WO 96/33978
(AstraZeneca); PCT WO96/3397 (AstraZeneca); PCT WO 96/33980
(AstraZeneca), gefitinib (IRESSA.TM., ZD1839, AstraZeneca), and
OSI-774 (Tarceva.TM., OSI Pharmaceuticals/Genentech), all patent
publications incorporated entirely by reference.
[0294] In another embodiment, the immunoglobulin is administered
with one or more immunomodulatory agents. Such agents may increase
or decrease production of one or more cytokines, up- or
down-regulate self-antigen presentation, mask MHC antigens, or
promote the proliferation, differentiation, migration, or
activation state of one or more types of immune cells.
Immunomodulatory agents include but not limited to: non-steroidal
anti-inflammatory drugs (NSAIDs) such as asprin, ibuprofed,
celecoxib, diclofenac, etodolac, fenoprofen, indomethacin,
ketoralac, oxaprozin, nabumentone, sulindac, tolmentin, rofecoxib,
naproxen, ketoprofen, and nabumetone; steroids (eg.
glucocorticoids, dexamethasone, cortisone, hydroxycortisone,
methylprednisolone, prednisone, prednisolone, trimcinolone,
azulfidineicosanoids such as prostaglandins, thromboxanes, and
leukotrienes; as well as topical steroids such as anthralin,
calcipotriene, clobetasol, and tazarotene); cytokines such as TGFb,
IFNa, IFNb, IFNg, IL-2, IL-4, IL-10; cytokine, chemokine, or
receptor antagonists including antibodies, soluble receptors, and
receptor-Fc fusions against BAFF, B7, CCR2, CCR5, CD2, CD3, CD4,
CD6, CD7, CD8, CD11, CD14, CD15, CD17, CD18, CD20, CD23, CD28,
CD40, CD40L, CD44, CD45, CD52, CD64, CD80, CD86, CD147, CD152,
complement factors (C5, D) CTLA4, eotaxin, Fas, ICAM, ICOS,
IFN.alpha., IFN.beta., IFNy, IFNAR, IgE, IL-1, IL-2, IL-2R, IL-4,
IL-5R, IL-6, IL-8, IL-9 IL-12, IL-13, IL-13R1, IL-15, IL-18R,
IL-23, integrins, LFA-1, LFA-3, MHC, selectins, TGF.beta.,
TNF.alpha., TNF.beta., TNF-R1, T-cell receptor, including
Enbrel.RTM. (etanercept), Humira.RTM. (adalimumab), and
Remicade.RTM. (infliximab); heterologous anti-lymphocyte globulin;
other immunomodulatory molecules such as 2-amino-6-aryl-5
substituted pyrimidines, anti-idiotypic antibodies for MHC binding
peptides and MHC fragments, azathioprine, brequinar, bromocryptine,
cyclophosphamide, cyclosporine A, D-penicillamine, deoxyspergualin,
FK506, glutaraldehyde, gold, hydroxychloroquine, leflunomide,
malononitriloamides (eg. leflunomide), methotrexate, minocycline,
mizoribine, mycophenolate mofetil, rapamycin, and
sulfasasazine.
[0295] In an alternate embodiment, immunoglobulins disclosed herein
are administered with a cytokine. By "cytokine" as used herein is
meant a generic term for proteins released by one cell population
that act on another cell as intercellular mediators. Examples of
such cytokines are lymphokines, monokines, and traditional
polypeptide hormones. Included among the cytokines are growth
hormone such as human growth hormone, N-methionyl human growth
hormone, and bovine growth hormone; parathyroid hormone; thyroxine;
insulin; proinsulin; relaxin; prorelaxin; glycoprotein hormones
such as follicle stimulating hormone (FSH), thyroid stimulating
hormone (TSH), and luteinizing hormone (LH); hepatic growth factor;
fibroblast growth factor; prolactin; placental lactogen; tumor
necrosis factor-alpha and -beta; mullerian-inhibiting substance;
mouse gonadotropin-associated peptide; inhibin; activin; vascular
endothelial growth factor; integrin; thrombopoietin (TPO); nerve
growth factors such as NGF-beta; platelet-growth factor;
transforming growth factors (TGFs) such as TGF-alpha and TGF-beta;
insulin-like growth factor-I and --II; erythropoietin (EPO);
osteoinductive factors; interferons such as interferon-alpha, beta,
and -gamma; colony stimulating factors (CSFs) such as
macrophage-CSF (M-CSF); granulocyte-macrophage-CSF (GM-CSF); and
granulocyte-CSF (G-CSF); interleukins (ILs) such as IL-1,
IL-1alpha, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10,
IL-11, IL-12; IL-15, a tumor necrosis factor such as TNF-alpha or
TNF-beta; and other polypeptide factors including LIF and kit
ligand (KL). As used herein, the term cytokine includes proteins
from natural sources or from recombinant cell culture, and
biologically active equivalents of the native sequence
cytokines.
[0296] In one embodiment, cytokines or other agents that stimulate
cells of the immune system are co-administered with the
immunoglobulin disclosed herein. Such a mode of treatment may
enhance desired effector function. For example, agents that
stimulate NK cells, including but not limited to IL-2 may be
co-administered. In another embodiment, agents that stimulate
macrophages, including but not limited to C5a, formyl peptides such
as N-formyl-methionyl-leucyl-phenylalanine (Beigier-Bompadre et al.
(2003) Scand. J. Immunol. 57: 221-8, incorporated entirely by
reference), may be co-administered. Also, agents that stimulate
neutrophils, including but not limited to G-CSF, GM-CSF, and the
like may be administered. Furthermore, agents that promote
migration of such immunostimulatory cytokines may be used. Also
additional agents including but not limited to interferon gamma,
IL-3 and IL-7 may promote one or more effector functions.
[0297] In an alternate embodiment, cytokines or other agents that
inhibit effector cell function are co-administered with the
immunoglobulin disclosed herein. Such a mode of treatment may limit
unwanted effector function.
[0298] In an additional embodiment, the immunoglobulin is
administered with one or more antibiotics, including but not
limited to: aminoglycoside antibiotics (e.g. apramycin, arbekacin,
bambermycins, butirosin, dibekacin, gentamicin, kanamycin,
neomycin, netilmicin, paromomycin, ribostamycin, sisomycin,
spectrinomycin), aminocyclitols (eg. sprctinomycin), amphenicol
antibiotics (eg. azidamfenicol, chloramphenicol, florfrnicol, and
thiamphemicol), ansamycin antibiotics (eg. rifamide and rifampin),
carbapenems (eg. imipenem, meropenem, panipenem); cephalosporins
(eg. cefaclor, cefadroxil, cefamandole, cefatrizine, cefazedone,
cefozopran, cefpimizole, cefpiramide, cefpirome, cefprozil,
cefuroxine, cefixime, cephalexin, cephradine ), cephamycins
(cefbuperazone, cefoxitin, cefminox, cefmetazole, and cefotetan);
lincosamides (eg. clindamycin, lincomycin); macrolide (eg.
azithromycin, brefeldin A, clarithromycin, erythromycin,
roxithromycin, tobramycin), monobactams (eg. aztreonam, carumonam,
and tigernonam); mupirocin; oxacephems (eg. flomoxef, latamoxef,
and moxalactam); penicillins (eg. amdinocillin, amdinocillin
pivoxil, amoxicillin, bacampicillin, bexzylpenicillinic acid,
benzylpenicillin sodium, epicillin, fenbenicillin, floxacillin,
penamecillin, penethamate hydriodide, penicillin o-benethamine,
penicillin O, penicillin V, penicillin V benzoate, penicillin V
hydrabamine, penimepicycline, and phencihicillin potassium);
polypeptides (eg. bacitracin, colistin, polymixin B, teicoplanin,
vancomycin); quinolones (amifloxacin, cinoxacin, ciprofloxacin,
enoxacin, enrofloxacin, feroxacin, flumequine, gatifloxacin,
gemifloxacin, grepafloxacin, lomefloxacin, moxifloxacin, nalidixic
acid, norfloxacin, ofloxacin, oxolinic acid, pefloxacin, pipemidic
acid, rosoxacin, rufloxacin, sparfloxacin, temafloxacin,
tosufloxacin, trovafloxacin); rifampin; streptogramins (eg.
quinupristin, dalfopristin); sulfonamides (sulfanilamide,
sulfamethoxazole); tetracyclenes (chlortetracycline, demeclocycline
hydrochloride, demethylchlortetracycline, doxycycline, duramycin,
minocycline, neomycin, oxytetracycline, streptomycin, tetracycline,
vancomycin).
[0299] Anti-fungal agents such as amphotericin B, ciclopirox,
clotrimazole, econazole, fluconazole, flucytosine, itraconazole,
ketoconazole, niconazole, nystatin, terbinafine, terconazole, and
tioconazole may also be used.
[0300] Antiviral agents including protease inhibitors, reverse
transcriptase inhibitors, and others, including type I interferons,
viral fusion inhibitors, and neuramidase inhibitors, may also be
used.
[0301] Examples of antiviral agents include, but are not limited
to, acyclovir, adefovir, amantadine, amprenavir, clevadine,
enfuvirtide, entecavir, foscarnet, gangcyclovir, idoxuridine,
indinavir, lopinavir, pleconaril, ribavirin, rimantadine,
ritonavir, saquinavir, trifluridine, vidarabine, and zidovudine,
may be used.
[0302] The antibodies disclosed herein may be combined with other
therapeutic regimens. For example, in one embodiment, the patient
to be treated with an immunoglobulin disclosed herein may also
receive radiation therapy. Radiation therapy can be administered
according to protocols commonly employed in the art and known to
the skilled artisan. Such therapy includes but is not limited to
cesium, iridium, iodine, or cobalt radiation. The radiation therapy
may be whole body irradiation, or may be directed locally to a
specific site or tissue in or on the body, such as the lung,
bladder, or prostate. Typically, radiation therapy is administered
in pulses over a period of time from about 1 to 2 weeks. The
radiation therapy may, however, be administered over longer periods
of time. For instance, radiation therapy may be administered to
patients having head and neck cancer for about 6 to about 7 weeks.
Optionally, the radiation therapy may be administered as a single
dose or as multiple, sequential doses. The skilled medical
practitioner can determine empirically the appropriate dose or
doses of radiation therapy useful herein. In accordance with
another, an immunoglobulin disclosed herein and one or more other
anti-cancer therapies are employed to treat cancer cells ex vivo.
It is contemplated that such ex vivo treatment may be useful in
bone marrow transplantation and particularly, autologous bone
marrow transplantation. For instance, treatment of cells or
tissue(s) containing cancer cells with immunoglobulin and one or
more other anti-cancer therapies, such as described above, can be
employed to deplete or substantially deplete the cancer cells prior
to transplantation in a recipient patient.
[0303] It is of course contemplated that the antibodies disclosed
herein may employ in combination with still other therapeutic
techniques such as surgery or phototherapy.
EXAMPLES
[0304] Examples are provided below are for illustrative purposes
only. These examples are not meant to constrain any embodiment
disclosed herein to any particular application or theory of
operation.
Example 1. Novel Methods for Inhibiting Fc.gamma.RIIb.sup.+
Cells
[0305] Fc.gamma.RIIb is expressed on a variety of immune cells,
including B cells, plasma cells, dendritic cells, monocytes, and
macrophages, where it plays a critical role in immune regulation.
In its normal role on B cells, Fc.gamma.RIIb serves as a feedback
mechanism to modulate B cell activation through the B cell receptor
(BCR). Engagement of B cell antigen receptor (BCR) by immune
complexed antigen on mature B cells activates an intracellular
signaling cascade, including calcium mobilization, which leads to
cell proliferation and differentiation. However, as IgG antibodies
with specificity to the antigen are produced, the associated immune
complexes (ICs) can crosslink the BCR with Fc.gamma.RIIb, whereupon
the activation of BCR is inhibited by engagement of Fc.gamma.RIIb
and associated intracellular signaling pathways that interfere with
the downstream pathways of BCR activation.
[0306] B cells function not only to produce antibodies and
cytokines that control immune response, they are also antigen
presenting cells (APCs). Internalization of antigen by BCR into a B
cell can play a role in presentation to and activation of T cells.
Regulation of B cell activation through the BCR is also potentially
regulated by antibody engagement of Fc.gamma.RIIb. Other APCs such
as dendritic cells, macrophages, and monocytes, are capable of
internalizing antibody-bound antigen through activating receptors
such as Fc.gamma.RIIa, Fc.gamma.RIIIa, and Fc.gamma.RI. Expression
of Fc.gamma.RIIb on these cell types, particularly dendritic cells,
can inhibit activation of these cell types and subsequent
presentation to and activation of T cells (Desai et al., 2007, J
Immunol).
[0307] A novel strategy for inhibiting activation of the
aforementioned cell types it to use a single immunoglobulin to
coengage Fc.gamma.RIIb with surface antigen present on the
Fc.gamma.RIIb+ cell. In the case of B cells, based on the natural
biological mechanism, this would potentially involve dual targeting
of Fc.gamma.RIIb and BCR, with the goal of mimicking immune
complex-mediated suppression of B cell activation. FIG. 3
illustrates one such potential mechanism, in which an antibody is
used to coengage both Fc.gamma.RIIb via its Fc region, and a target
antigen associated with BCR complex, in this example CD19, via its
Fv region.
Example 2. Engineering Immunoglobulins with Selectively Enhanced
Affinity for Fc.gamma.RIIb
[0308] Under physiological conditions, bridging of the BCR with
Fc.gamma.RIIb and subsequent B cell suppression occurs via immune
complexes of IgGs and cognate antigen. The design strategy was to
reproduce this effect using a single crosslinking antibody. Human
IgG binds human Fc.gamma.RIIb with weak affinity (approximately 1
.mu.M for IgG1), and Fc.gamma.RIIb-mediated inhibition occurs in
response to immune-complexed but not monomeric IgG. It was reasoned
that increasing Fc affinity to this receptor would be required for
maximal inhibition of B cell activation. Protein engineering
methods were used to design and screen Fc variants for enhanced
Fc.gamma.RIIb binding.
[0309] In addition to this primary design goal (maximal Fc affinity
to Fc.gamma.RIIb), a secondary design goal was to reduce
interaction of the Fc region with activating Fc.gamma.Rs.
Fc.gamma.R affinity profiles that may be optimal for inhibiting
Fc.gamma.RIIb cells include not only high affinity for the
inhibitory receptor Fc.gamma.RIIb, but also potentially high
Fc.gamma.RIIb affinity coupled with reduced affinity for one or
more activating receptors, including, for example, Fc.gamma.RI,
Fc.gamma.RIIIa, and/or Fc.gamma.RIIa. Reduced affinity to
activating receptors may lead to reduced toxicity associated with
an antibody treatment. For example, reduced affinity to
Fc.gamma.RIIIa, present on NK cells, should reduce the level of NK
cell-mediated ADCC. Similarly, reduced affinity to Fc.gamma.RIIa,
present on a variety of effector cells including macrophages and
neutrophils, should reduce the level of phagocytosis (ADCP)
mediated by these cells. In addition, for monocytes, macrophages,
dendritic cells, and the like, reduced interaction with activating
Fc.gamma.Rs would mean that immunoglobulin would be more free to
interact with Fc.gamma.RIIb on the cell surface.
[0310] Using solved structures of the human Fc/Fc.gamma.RIIIb
complex (and the sequences of the human Fc.gamma.Rs, structural and
sequence analysis were used to identify Fc.gamma.R positions that
contribute to Fc.gamma.RIIb affinity and selectivity relative to
the activating receptors. The design strategy employed two steps.
First, Fc.gamma.R positions that are determinants of Fc.gamma.RIIb
and Fc.gamma.RIIIa binding selectivity were identified by
accounting for proximity to the Fc.gamma.R/Fc interface and amino
acid dissimilarity between Fc.gamma.RIIb and Fc.gamma.RIIIa. The
results of this analysis are presented in FIG. 4. Second, sequence
positions in the Fc region proximal to these Fc.gamma.R positions
were identified. The results of this analysis are presented in FIG.
5. Fc variants were designed that incorporate substitutions at
these positions.
[0311] A library of Fc variants was generated and screened to
explore amino acid modifications at these positions. Variants were
generated and screened in the context of an antibody targeting the
antigen CD19, a regulatory component of the BCR coreceptor complex.
The Fv region of the this antibody is a humanized and affinity
matured version of antibody 4G7, and is referred to herein as
HuAM4G7. The amino acid sequences of this antibody are provided in
FIG. 54A-FIG. 54D. The Fv genes for this antibody were subcloned
into the mammalian expression vector pTT5 (National Research
Council Canada). Mutations in the Fc domain were introduced using
site-directed mutagenesis (QuikChange, Stratagene, Cedar Creek,
Tex.). In addition, control knock out variants with ablated
affinity for Fc receptors were generated that comprise the
substitution L328R, and either a G236R substitution or an Arg
inserted after position 236. These variants (G236R/L328R and
236R/L328R) are referred to as Fc-KO or Fc.gamma.R knockout. To
serve as non-CD19 Fc isotype controls, anti-respiratory syncytial
virus (RSV) and anti-FITC antibodies were constructed in the pTT5
vector by fusing the appropriate V.sub.L and V.sub.H regions to the
C.sub.L.kappa. and C.sub.H1-3 domains with Fc changes. Heavy and
light chain constructs were cotransfected into HEK293E cells for
expression, and antibodies were purified using protein A affinity
chromatography (Pierce Biotechnology, Rockford, III.).
[0312] Human Fc receptor proteins Fc.gamma.RI and Fc.gamma.RIIb for
binding and competition studies were obtained from R&D Systems
(Minneapolis, Minn.). Genes encoding Fc.gamma.RIIa and
Fc.gamma.RIIIa receptor proteins were obtained from the Mammalian
Gene Collection (ATCC), and subcloned into pTT5 vector (National
Research Council Canada) containing 6.times. His and GST-tags.
Allelic forms of the receptors (H131 and R131 for Fc.gamma.RIIa and
V158 and F158 for Fc.gamma.RIIIa) were generated using QuikChange
mutagenesis. Vectors encoding the receptors were transfected into
HEK293T cells, and proteins were purified using nickel affinity
chromatography.
[0313] Variants were screened for receptor affinity using
Biacore.TM. technology, also referred to as Biacore herein, a
surface plasmon resonance (SPR) based technology for studying
biomolecular interactions in real time. SPR measurements were
performed using a Biacore 3000 instrument (Biacore, Piscataway,
N.J.). A protein A/G (Pierce Biotechnology) CM5 biosensor chip
(Biacore) was generated using a standard primary amine coupling
protocol. All measurements were performed using HBS-EP buffer (10
mM HEPES pH 7.4, 0.15 M NaCl, 3 mM EDTA, 0.005% vol/vol surfactant
P20, Biacore). Antibodies at 20 nM or 50 nM in HBS-EP buffer were
immobilized on the protein A/G surface and Fc.gamma.Rs were
injected. After each cycle, the surface was regenerated by
injecting glycine buffer (10 mM, pH 1.5). Data were processed by
zeroing time and response before the injection of Fc.gamma.R and by
subtracting appropriate nonspecific signals (response of reference
channel and injection of running buffer). Kinetic analyses were
performed by global fitting of binding data with a 1:1 Langmuir
binding model using BIAevaluation software (Biacore).
[0314] A representative set of sensorgrams for binding of select
variant anti-CD19 antibodies to Fc.gamma.RIIb is shown in FIG. 6.
The affinities (equilibrium Kds) of all variants and WT (native)
IgG1 to all of the Fc.gamma.Rs, obtained from fits of the Biacore
binding data, are presented in FIG. 7A-FIG. 7D. Whereas WT IgG1 Fc
binds with Fc.gamma.RIIb with M affinity (Kd=1467 nM in FIG. 7), a
large number of variants have been engineered that bind more
tightly. Because all of the antibodies tested have specificity for
CD19 (via their Fv region), the binding results in FIG. 7A-FIG. 7D
are due solely to binding to Fc.gamma.RIIb by the Fc region. This
is supported by the lack of detectable binding by the Fc-KO
variants (G236R/L328R and 236R/L328R), which are ablated for
binding to all Fc.gamma.Rs.
[0315] A useful quantity for analysis of the variants is their fold
affinity relative to WT IgG1, which is generated by dividing the Kd
for binding of WT IgG1 by the Kd for binding of variant for each
receptor. These fold affinity results are provided in FIG. 8A-FIG.
8D. A number of variants have Fc.gamma.RIIb binding enhancements
over 2 logs, and substantially reduced or ablated affinities for
the activating receptors. In particular, S267E (single
substitution) as well as L235Y/S267E, G236D/S267E, S239D/S267E,
S267E/H268E, and S267E/L328F (double substitutions) have markedly
higher affinity for Fc.gamma.RIIb. In addition, these variant have
affinity for the activating receptor Fc.gamma.RIIIa that is either
comparable to native IgG1, modestly enhanced, or even significantly
reduced.
[0316] FIG. 9 shows a plot of the Fc.gamma.R affinities of select
variants on a log scale, compared with those of WT IgG1. The
variant with the highest affinity for Fc.gamma.RIIb, S267E/L328F,
shows over 2 orders of magnitude improvement in affinity to
Fc.gamma.RIIb, and significantly reduced affinity to the activating
receptors, including Fc.gamma.RIIIa, Fc.gamma.RI, and H131
Fc.gamma.RIIa.
[0317] The data in FIGS. 9 and 10 indicate that the properties of
the variants are highly dependent not only on the position that is
mutated, but also the precise amino acid that is substituted at
each position. For example, one of the strongest positions for
controlling Fc.gamma.RIIb affinity and selectivity relative to
activating Fc.gamma.Rs is position 267. Yet modification at this
position can yield dramatically different results depending on the
particular amino acid that is substituted In particular, as shown
in FIG. 10, whereas affinity of S267E for Fc.gamma.RIIb is greatly
enhanced relative to WT IgG1 and provides substantial selectivity
improvement relative to Fc.gamma.RIIIa, other substitutions such as
S267A and S267G provide either marginal or no Fc.gamma.RIIb
enhancement, and/or little or no selectivity improvement relative
to Fc.gamma.RIIIa. The importance of the precise modification is
further supported by the fact that two of the best positions for
selectively enhancing Fc.gamma.RIIb affinity, 236 and 328 (for
example 236D and 328F) are also the same positions that are
modified to generate the Fc-KO variant (236R and 328R).These
results illustrate the complexity of the Fc.gamma.R interface, and
highlight the challenge of engineering modifications that precisely
control desired Fc.gamma.R properties.
[0318] Many of the Fc combination variants, including double and
triple combinations of single substitutions, exhibited unexpected
synergy (non-additivity) when compared against the single
substitutions alone. This was determined (for all combination
variants for which data was available) by comparing the actual fold
improvement in affinity as measured by Biacore versus the expected
fold improvement in affinity as calculated by the product of the
fold improvements of the single substitutions (FIG. 11). As can be
seen from the data, double substitutions at the following pairs of
positions resulted in a greater than expected affinity for one or
more Fc.gamma.Rs: 234/267, 235/267, 236/267, 236/268, 239/267,
239/268, 266/267, 267/328, and 268/327.
[0319] In order to validate the Biacore data and evaluate receptor
binding of the variants on the cell surface, binding of select
antibodies to cells expressing Fc.gamma.RIIb was measured. Since
HEK293T cells do not express CD19 or Fc.gamma.Rs, transfection of
these cells with Fc.gamma.RIIb allowed an analysis of antibody
binding to Fc receptors in an isolated system on a cell surface.
HEK293T cells in DMEM with 10% FBS were transfected with human
Fc.gamma.RIIb cDNA in pCMV6 expression vector (Origene
Technologies, Rockville, Md.), cultured for 3 days, harvested,
washed twice in PBS, resuspended in PBS with 0.1% BSA (PBS/BSA),
and aliquoted at 2.times.105 cells per well into 96-well microtiter
plates. Fc variant antibodies were serially diluted in PBS/BSA then
added to the cells and incubated with mixing for 1 h at room
temperature. After extensive washing with PBS/BSA, phycoerythrin
(PE)-labeled anti-human-Fab-specific goat F(ab')2 fragment was
added for detection. Cells were incubated for 30 min at room
temperature, washed, and resuspended in PBS/BSA. Binding was
evaluated using a FACSCanto II flow cytometer (BD Biosciences, San
Jose, Calif.), and the mean fluorescence intensity (MFI) was
plotted as a function of antibody concentration using GraphPad
Prism software (GraphPad Software, San Diego, Calif.) from which
half-maximal binding (EC50) values were determined by sigmoidal
dose response modeling.
[0320] Receptor expression levels were assessed prior to binding of
antibodies, and half-maximal effective concentration (EC50) values
of the MFI at different antibody concentrations were determined.
FIG. 12 shows the results of this experiment. The EC50 values of
the variants tested showed a similar rank order as the Biacore
results. The cell-surface binding confirmed that the S267E/L328F
variant of those tested has the highest affinity for Fc.gamma.RIIb,
with an EC50 approximately 320-fold relative to WT IgG1. The strong
agreement between these cell surface binding data and the Biacore
binding data support the accuracy of the affinity measurements.
[0321] Because of the importance of animal models in drug
development, select variants were screened further for binding to
mouse and cynomolgous monkey receptors. The extracellular regions
of mouse and cynologous monkey (Macaca fascicularis) Fc.gamma.Rs
were expressed and purified. The extracellular regions of these
receptors were obtained by PCR from clones obtained from the
Mammalian Gene Collection (MGC), or generated de novo using
recursive PCR. To enable purification and screening, receptors were
fused C-terminally with a His- and GST-tag. Tagged Fc.gamma.Rs were
transfected into 293T cells, and media containing secreted receptor
were harvested 3 days later and purified using Nickel
chromatography.
[0322] Variant antibodies were tested for their affinity to mouse
or cynologous monkey Fc.gamma.Rs using Biacore SPR as described
above. Specifically, antibodies were first immobilized on a protein
A/G chip to high density, and then followed by injections of the
extracellular domain of the mouse or cynologous monkey Fc.gamma.R
of interest. Both association and dissociation phases were tracked
in real time using the Biacore technology. FIG. 13A-FIG. 13D show
the fold improvements (compared to WT IgG1) for binding of select
variants to mouse and cynologous monkey Fc.gamma.Rs as determined
from Biacore.
[0323] Although the variants were screened in the context of human
IgG1, it is contemplated that the variants could be used in the
context of other antibody isotypes, for example including but not
limited to human IgG2, human IgG3, and human IgG4 (FIG. 1). In
order to explore the transferability of the variants to other
antibody isotypes, the S267E/L328F variant was constructed and
tested in the context of a IgG1/2 ELLGG antibody, which is a
variant of an IgG2 Fc region (U.S. Ser. No. 11/256,060, herein
expressly incorporated by reference). The mutations were
constructed, antibodies purified, and binding data carried out as
described above. FIG. 14 shows affinities of the IgG1 and IgG1/2
variant antibodies to the human Fc.gamma.Rs as determined by
Biacore. The data indicate that the greatly enhanced Fc.gamma.RIIb
affinity and the overall Fc.gamma.R binding profile are maintained
in the variant IgG2 Fc region, thus supporting the use of the
variants in other isotype contexts.
[0324] Collectively, the above data indicate that a number of
engineered variants, at specific Fc positions, provide the targeted
properties, namely enhanced affinity for Fc.gamma.RIIb, and
selectively enhanced Fc.gamma.RIIb affinity relative to the
activating receptors Fc.gamma.RI, Fc.gamma.RIIa, and
Fc.gamma.RIIIa. Substitutions to enhance affinity to Fc.gamma.RIIb
include: 234, 235, 236, 237, 239, 266, 267, 268, 325, 326, 327,
328, and 332. In some embodiments, substitutions are made to at
least one or more of the nonlimiting following positions to enhance
affinity to Fc.gamma.RIIb: 235, 236, 239, 266, 267, 268, and
328.
[0325] Nonlimiting combinations of positions for making
substitutions to enhance affinity to Fc.gamma.RIIb include:
234/239, 234/267, 234/328, 235/236, 235/239, 235/267, 235/268,
235/328, 236/239, 236/267, 236/268, 236/328, 237/267, 239/267,
239/268, 239/327, 239/328, 239/332, 266/267, 267/268, 267/325,
267/327, 267/328, 267/332, 268/327, 268/328, 268/332, 326/328,
327/328, and 328/332. In some embodiments, combinations of
positions for making substitutions to enhance affinity to
Fc.gamma.RIIb include, but are not limited to: 235/267, 236/267,
239/268, 239/267, 267/268, and 267/328.
[0326] Substitutions for enhancing affinity to Fc.gamma.RIIb
include: L234D, L234E, L234W, L235D, L235F, L235R, L235Y, G236D,
G236N, G237D, G237N, S239D, S239E, V266M, S267D, S267E, H268D,
H268E, A327D, A327E, L328F, L328W, L328Y, and 1332E. In some
embodiments, combination of positions for making substitutions for
enhancing affinity to Fc.gamma.RIIb include, but are not limited
to: L235Y, G236D, S239D, V266M, S267E, H268D, H268E, L328F, L328W,
and L328Y.
[0327] Combinations of substitutions for enhancing affinity to
Fc.gamma.RIIb include: L234D/S267E, L234E/S267E, L234F/S267E,
L234E/L328F, L234W/S239D, L234W/S239E, L234W/S267E, L234W/L328Y,
L235D/S267E, L235D/L328F, L235F/S239D, L235F/S267E, L235F/L328Y,
L235Y/G236D, L235Y/S239D, L235Y/S267D, L235Y/S267E, L235Y/H268E,
L235Y/L328F, G236D/S239D, G236D/S267E, G236D/H268E, G236D/L328F,
G236N/S267E, G237D/S267E, G237N/S267E, S239D/S267D, S239D/S267E,
S239D/H268D, S239D/H268E, S239D/A327D, S239D/L328F, S239D/L328W,
S239D/L328Y, S239D/1332E, S239E/S267E, V266M/S267E, S267D/H268E,
S267E/H268D, S267E/H268E, S267E/N325L, S267E/A327D, S267E/A327E,
S267E/L328F, S267E/L328I, S267E/L328Y, S267E/1332E, H268D/A327D,
H268D/L328F, H268D/L328W, H268D/L328Y, H268D/1332E, H268E/L328F,
H268E/L328Y, A327D/L328Y, L328F/1332E, L328W/1332E, and
L328Y/1332E. In some embodiments, combinations of substitutions for
enhancing affinity to Fc.gamma.RIIb include, but are not limited
to: L235Y/S267E, G236D/S267E, S239D/H268D, S239D/S267E,
S267E/H268D, S267E/H268E, and S267E/L328F.
Example 3. Immunoqlobulins Inhibit BCR-Mediated Primary Human B
Cell Viability
[0328] Although normal B cells have a long in vivo half life of
approximately five weeks, their lifespan is greatly reduced in
vitro. BCR stimulation by crosslinking antibodies such as anti-IgM
or anti-CD79b counteracts this in vitro predisposition towards
apoptosis, leading to B cell activation and increased B cell
viability. To demonstrate this, an ATP-dependent B cell viability
assay was performed. Human peripheral blood mononuclear cells
(PBMCs) were purified from leukapheresis of anonymous healthy
volunteers (HemaCare, Van Nuys, Calif.) using Ficoll-Paque Plus
density gradients (Amersham Biosciences, Newark, N.J.). Primary
human B cells were purified from PBMCs using a B cell enrichment
kit (StemCell Technologies, Vancouver, British Columbia). Murine
anti-human CD79b (clone SN8) was purchased from Santa Cruz
Biotechnology (Santa Cruz, Calif.). Polyclonal anti-mu F(ab')2 was
purchased from Jackson Immunoresearch Lab (West Grove, Pa.).
Anti-mu or anti-CD79b antibody serial dilutions were performed in
triplicate in 96-well microtiter plates containing RPMI1640 with
10% FBS. Purified primary human B cells (5-7.5.times.104 per well)
were added to a final volume of 100 p1, and incubated at 37.degree.
C. for 3 days. ATP-dependent luminescence was quantified to
determine cell viability (Cell Titer-Glo Cell Viability Assay,
Promega, Madison, Wis.) and a Topcount luminometer (PerkinElmer,
Waltham, Mass.) was used for data acquisition. FIGS. 15A and 15B
show the results of the assay, demonstrating the survival of
primary human B cells upon BCR activation, here carried out by
crosslinking with anti-mu (A) or anti-CD79b (B) antibodies. In vivo
such activation would occur via immune complexed antigen, which for
example could be an infectious agent, or in the cause of an
autoimmune or allergic reaction could be an anutoimmune antigen or
allergen.
[0329] The ATP-dependent luminescence assay was used to examine if
BCR activation-mediated viability of primary human B cells could be
suppressed by an anti-CD19 antibody having enhanced Fc affinity for
Fc.gamma.RIIb. The above experiment was repeated, except that
antibody serial dilutions of WT, variant, and control antibodies
were performed in triplicate in 96-well microtiter plates
containing RPMI1640 with 10% FBS, plus anti-CD79b at 1 .mu.g/ml to
stimulate BCR. The results are shown in FIG. 16. Again, B cells
possessed low viability in the absence of BCR crosslinking, and
addition of 10 .mu.g/ml anti-CD79b antibody stimulated survival by
about 6-fold (cells alone vs. anti-CD79b). Anti-CD19-S267E/L328F,
the variant with the highest Fc.gamma.RIIb affinity, inhibited
BCR-stimulated viability in a dose-dependent manner. In contrast,
control antibodies including anti-CD19-IgG1 (Fv control) and
anti-RSV-S267E/L328F (Fc control) minimally suppressed viability.
To assess if this inhibitory effect required coengagement of CD19
and Fc.gamma.RIIb, as opposed to simultaneous binding of each
receptor by different antibodies, the anti-CD19-S267E/L328F variant
was compared to a combination of anti-CD19-IgG1 and
anti-RSV-S267E/L328F controls at equal concentrations. The
combination of these antibodies should simultaneously bind to both
CD19 and Fc.gamma.RIIb but, unlike anti-CD19-S267E/L328F, is unable
to crosslink these receptors. As shown in FIG. 16, the combination
failed to suppress BCR activation-induced survival, indicating that
coengagement of Fc.gamma.RIIb and CD19 by a single molecule is
required to inhibit BCR-mediated viability. Not all variants were
capable of inhibiting B cell activation. As demonstrated in FIG.
17, variants with moderately increased affinity relative to WT IgG1
(S267A, 408 nM, 3.6-fold relative to native IgG1) do not inhibit B
cell activation. In contrast, that data in FIG. 18 demonstrate that
variants with high affinity, here the weakest affinity being the
S267E variant (71.9 nM, 20.4-fold relative to native IgG1), do
indeed inhibit activation. Together the results in FIGS. 18, 19,
and 20 suggest that a certain high affinity for Fc.gamma.RIIb,
about 100 nM, is needed to mediate inhibitory activity upon
coengagement of Fc.gamma.RIIb and BCR co-receptor target
antigen.
Example 4. Immunoqlobulins Inhibit BCR Activation of Calcium
Mobilization in Primary Human B Cells Via Coenqaqement of
Fc.gamma.RIIb and CD19
[0330] Signals through the B-cell receptor complex ultimately
result in calcium release, and this pathway can be inhibited by
Fc.gamma.RIIb (Nielsen et al., 2005, Transfus Med Hemother
32:339-347, incorporated entirely by reference). Intracellular
calcium mobilization was used as a quantitative measure of
BCR-mediated B cell activation to further evaluate the impact of
the immunoglobulins. The current study used primary B cells from
normal human donors as a more physiologically relevant model of
calcium signaling. In addition, rather than stimulating primarily
naive B cells via an anti-IgM antibody, an anti-human CD79b
(Ig.beta.) antibody was used in order to induce BCR activation in
both naive and memory B cells.
[0331] Intracellular free calcium concentration ([Ca2+]) was
measured by flow cytometry using a Fluo-4 NW calcium assay
(Molecular Probes, Eugene, Oreg.). Purified human B cells were
resuspended at 5.times.105 cells/ml in calcium assay buffer and
pre-loaded with Fluo-4 dye for 30 min at room temperature. After
incubation with anti-CD19 or Fc and Fv control antibodies, cells
were stimulated by addition of 10 .mu.g/ml of anti-CD79b antibody.
Calcium flux kinetics was recorded using a FACSCanto II flow
cytometer and data were analyzed using FlowJo software (Tree Star,
Ashland, Oreg.).
[0332] Calcium mobilization in the presence of 10 .mu.g/ml
anti-CD19 native IgG1 Fc antibody (a-CD19-native-IgG1) was
increased relative to the vehicle control (FIG. 19), as expected
from coengagement of CD19 and BCR. In contrast, IIbE variants of
anti-CD19 IgG1 (also at 10 .mu.g/ml) inhibited calcium mobilization
induced by BCR crosslinking, with the two highest-affinity variants
showing greatest activity. To determine the importance of CD19
binding for this effect, an Fc isotype control antibody was used
that binds with high affinity to Fc.gamma.RIIb but not to CD19;
this antibody, referred to as .alpha.-FITC-S267E/L328F in FIG. 19,
has the S267E/L328F IgG1 heavy chain, but an Fv region that binds
the hapten FITC (which is not on B cells). Relative to vehicle,
this antibody had minimal effect on calcium mobilization,
indicating that CD19 binding is required to inhibit calcium
mobilization. A dose-response extension of this experiment was
carried out in which each point represents the area under the curve
of a single calcium mobilization response as in FIG. 19. The data
show that potency and efficacy of IIbE variants correlated with
affinity for Fc.gamma.RIIb, consistent with the B cell viability
assay, with anti-CD19-Native-IgG1 showing no dose response (FIG.
20). The relationship between the EC50 of calcium inhibition and
affinity for Fc.gamma.RIIb is shown in FIG. 21.
[0333] To assess if the observed inhibition of calcium flux
required engagement of both Fc.gamma.RIIb and CD19 by a single
antibody, a competition experiment was performed. Because
Fc.gamma.RI has the highest affinity among all the Fc.gamma.Rs
(FIG. 9) and competes with Fc.gamma.RIIb for IgG binding (data not
shown), a 24-fold molar excess soluble Fc.gamma.RI (soIFc.gamma.RI)
to block the interaction of the highest affinity antibody
(.alpha.-CD19-S267E/L328F) with Fc.gamma.RIIb (FIG. 22).
BCR-induced calcium mobilization was again effectively inhibited by
10 .mu.g/ml .alpha.-CD19-S267E/L328F, but not by
a-CD19-Native-IgG1. Notably, inhibition by the IIbE variant was
completely abolished in the presence of soluble Fc.gamma.RI,
indicating that Fc.gamma.RIIb engagement is required. These results
indicate that BCR-induced calcium mobilization can be inhibited by
a single antibody that binds with high affinity to both
Fc.gamma.RIIb and CD19 surface receptors.
[0334] Together the B cell viability and calcium mobilization
results suggest that Fc variant antibodies with high affinity for
Fc.gamma.RIIb may be useful in methods for inhibiting activation of
B cells. The data provided indicate that amino acid modification at
positions 234, 235, 236, 237, 239, 266, 267, 268, 325, 326, 327,
328, and 332 may be useful for such inhibitory methods. In
particular, the data provided indicate that substitutions 234D,
234E, 234W, 235D, 235F, 235R, 235Y, 236D, 236N, 237D, 237N, 239D,
239E, 266M, 267D, 267E, 268D, 268E, 327D, 327E, 328F, 328W, 328Y,
and I332E may be useful for such inhibitory methods.
Example 5. Immunoqlobulins Induce SHIP Phosphorylation
[0335] SHIP activation is an Fc.gamma.RIIb-dependent downstream
component of ITIM-associated signaling. The capacity of the
anti-CD19-S267E/L328F antibody to stimulate SHIP phosphorylation
(pSHIP) in the context of BCR activation by anti-CD79b crosslinking
antibody was assessed using western analysis. Purified primary
human B cells (1.times.107) were incubated for 10 min at 22.degree.
C. with 20 .mu.g/ml anti-CD79b and 10 .mu.g/ml anti-CD19
antibodies, and then ice-cold PBS was added. For the positive
control, 10 .mu.g/ml of anti-Fc.gamma.RII-specific antibody (AT10)
(AbD Serotec (Raleigh, N.C.) was used to crosslink Fc.gamma.RIIb,
and 20 .mu.g/ml anti-mouse IgG Fc.gamma.-specific antibody was used
to crosslink AT10 and anti-CD79b. Cells were lysed in cold RIPA
buffer (Cell Signaling, Beverly, Mass.) containing protease (Roche,
Indianapolis, Ind.) and phosphatase (Calbiochem, San Diego, Calif.)
inhibitor cocktails with 2 nM microcystin (Calbiochem), and
incubated for 30 min on ice. Lysates were centrifuged at 10,000 g
for 30 min at 4.degree. C. to remove debris, fractionated by
SDS-PAGE (NuPAGE Novex, Invitrogen Life Technologies, Carlsbad,
Calif.) and transferred to polyvinylidene difluoride membrane
(Invitrogen Life Technologies). Western analysis was performed with
phospho-SHIP (Cell Signaling Technologies, Beverly, Mass.) and
GAPDH-specific primary antibodies (Biovision, Mountain View,
Calif.) using HRP-conjugated anti-mouse IgG secondary antibody with
enhanced chemiluminescence imaging (Amersham Bioscience, Newark,
N.J.) and a UVP Bioimaging image capturing system (Upland,
Calif.).
[0336] The data are presented in FIG. 23. The western blot of cell
extracts from purified primary human B cells showed that the
anti-CD19 IIbE variant stimulated a substantial increase in pSHIP
level compared to anti-CD19 IgG1 and other controls
(anti-RSV-S267E/L328F and anti-CD19-Fc-KO) (FIG. 23, lane 1 vs.
lanes 2-4). As expected, direct crosslinking of Fc.gamma.RIIb with
BCR by anti-Fc.gamma.RII antibody also showed an increase in pSHIP
level (lane 5). These results indicate that suppression of B cell
function by the anti-CD19 IIbE antibody stimulates SHIP
phosphorylation, which is consistent with a known signaling pathway
of BCR-Fc.gamma.RIIb coengagement.
Example 6. Immunoqlobulins Inhibit BCR-Dependent Anti-Apoptotic
Effect in Primary Human B Cells
[0337] Although normal B cells in vivo have a long half life of
approximately 5 weeks, in vitro this lifespan is greatly reduced,
with increased apoptosis due to the lack of appropriate niche. B
cell activation via stimulation via the BCR induces an
anti-apoptotic effect and prolongs viability, as demonstrated in
FIG. 15. In order to determine whether the antiproliferative
activity of the IIbE variant was a result of neutralizing
BCR-mediated survival signals, thereby allowing in vitro apoptosis
to proceed, an annexin-V staining assay was performed. 1.times.105
purified primary human B cells were incubated for 24 h at
37.degree. C. in triplicate with 1 .mu.g/ml anti-CD79b and serial
dilutions of anti-CD19 or control antibodies in 100 .mu.l RPMI1640
with 10% FBS. After incubation, cells were harvested and stained
with PE-conjugated annexin-V (Biovision, Mountain View, Calif.) and
7-amino-actinomycin D (7-AAD, Invitrogen, Carlsbad, Calif.) at 5
.mu.g/ml. The annexin-V-positive/7-AAD-negative cells were acquired
using a FACSCanto II flow cytometer, and analyzed with FACSDiva 5
analysis software (BD Biosciences).
[0338] The data are shown in FIG. 24. Annexin-V staining of primary
human B cells cultured in the presence or absence of anti-CD79b
confirmed that apoptosis was suppressed by BCR activation (FIG. 24,
cells alone vs. anti-CD79b). This survival signal was neutralized
in a dose-dependent manner by anti-CD19-S267E/L328F, but not by
anti-RSV-S267E/L328F Fc control or anti-CD19-IgG1 Fv control
antibodies. Inhibition of the anti-apoptotic effect, like
inhibition of calcium mobilization and cell proliferation, requires
coengagement of CD19 and Fc.gamma.RIIb by a single antibody,
because the combination of anti-CD19-IgG1 and anti-RSV-S267E/L328F
(Fv and Fc controls, respectively) did not stimulate apoptosis.
These data indicate that the anti-CD19 IIbE variant inhibits
BCR-induced B cell proliferation by suppressing anti-apoptotic
survival signals.
Example 7. Immunoqlobulins do not Mediate Effector Functions
[0339] In order to evaluate the effect of modulating Fc.gamma.RIIIa
affinity, the immunoglobulins were examined for their ADCC
activity. Antibody serial dilutions were carried out in 96 well
microtiter plates in triplicates and incubated with Ramos target
cells (10,000 total) to opsonize the target cells for .about.15
minutes. Ramos is an immortal huma B cell line derived from
Burkitt's lymphoma cells. Purified NK cells (50,000 total) using
negative selection kit from frozen PBMC prepared from leukophoresis
pack using standard Ficoll density gradient were added to
appropriate concentration. The final working ADCC reaction was in
100 ul of 1% FBS/RPMI1640 for 4 hours at 37.degree. C. after which,
the amount of LDH released from the target cells was detected using
fluorescent detection system. The percentage of ADCC was determined
by normalizing the background LDH activity (target and NK together
without antibody) adjusted experimental LDH activity against the
total LDH activity present in the target cells (spontaneous LDH
activity present in the target cells alone adjusted TritonX100
lysed target cells). As shown in FIG. 25, many of the variants with
enhanced Fc.gamma.RIIb affinity, yet lower or equivalent
Fc.gamma.RIIIa affinity compared with wild-type IgG1, including
S267E, G236D/S267E, and S267E/L328F, lack ADCC activity. This is
attributed to their reduced or ablated affinity for the activating
Fc.gamma.Rs, particularly Fc.gamma.RIIIa which is the sole
Fc.gamma.R expressed on NK cells.
[0340] Immunoglobulins were also tested for their capacity to
mediate phagocytosis by macrophages. Target cells were RS4;11cells,
an immortal human B cell line derived from leukemia cells.
Macrophages express a variety of Fc.gamma.Rs, including
Fc.gamma.RI, Fc.gamma.RIIa, Fc.gamma.RIIb, and Fc.gamma.RIIa.
Purified monocytes were differentiated in the presence of
macrophage-colony stimulating factor for 5 days into macrophages.
Macrophages were mixed with fluorescently labeled (PKH26)
RS4;11cells in 10% human AB serum in RPMI followed by the addition
of anti-CD19 antibodies and incubated for 4 hours at 37.degree. C.
APC conjugated antibodies to CD14 and CD11b were added to the cell
mixture, washed and fixed. Phagocytosis was determined by the
percentage CD14+CD11b+ and PKH26 double positive population divided
by the total number of stained tumors. The data are shown in FIG.
26. Anti-CD19 IgG1 and the variant S239D/I332E demonstrated
phagocytosis. In contrast, variants with enhanced Fc.gamma.RIIb
affinity yet reduced affinity for activating receptors, including
S267E/L328F and G236D/S267E, had little or not phagocytic activity,
comparable to control antibody that targeted RSV.
[0341] Immunoglobulins were also tested for their capacity to
mediate CDC. Release of Alamar Blue was used to monitor lysis of a
target B cell line by human serum complement. Raji cells (an
immortal B cell line) were washed in 10% FBS medium by
centrifugation and resuspension, and loaded into 96-well plates at
40,000 cells per well. Variant anti-CD19 antibodies or Rituxan
anti-CD20 control were added in 1/2 fold dilutions to the indicated
final concentrations. Human serum complement (Quidel) was diluted 1
to 5 with medium and added to antibody-opsonized target cells.
Plates were incubated for 2 hrs at 37.degree. C., Alamar Blue was
added, cells were cultured overnight, and fluorescence was
measured. Data from this assay are shown in FIG. 27. In contrast to
the anti-CD20 control, the variant anti-CD19 antibodies do not
mediate CDC activity against B cells.
Example 8. In Vivo Data Demonstrating Potential for Treating
Autoimmune or Inflammatory Disorder
[0342] A hallmark of autoimmunity in mouse and human is
dysregulation of Fc.gamma.RIIb expression resulting in lower
surface level of this inhibitory receptor, leading to an elevated
level of B cell activation and consequential failure of
self-reactive B cell inhibition and production of plasma cells
secreting self-antigen specific immunoglobulins. Such self-reactive
immunoglobulin immune complexes are etiologic agents in various
organ failures in systemic autoimmunity and other arthritic
inflammations such as systemic lupus erythematosus (SLE) and
rheumatoid arthritis (RA. The immunoglobulins disclosed herein were
assessed using a huPBL-SCID mouse model as a proxy, by examining B
cell activity measured by the number of B cells and plasma cell
development by detecting the antigen specific immunoglobulins. In
this method, human PBLs from normal or diseased (e.g., SLE or RA)
donors are engrafted to immune-deficient SCID mice and treated with
the inhibitory immunoglobulin described herein, then challenged
with an antigen to examine the course of B cell development into
plasma cells. In such case, the production of antigen-specific
immunoglobulins is inhibited from which can be inferred inhibition
of both B cell activation and differentiation.
[0343] The protocol for this study is provided in FIG. 28A. Four
different groups of mice with five mice in each group were
engrafted with human PBLs from a healthy donor. At day 16, test
articles consisting of PBS (vehicle control), anti-CD19 with native
IgG1 Fc (anti-CD19 IgG1 WT), anti-CD19 with IgG1 Fc of enhanced
affinity for Fc.gamma.RIIb (anti-CD19 S267E/L328F) or Rituximab
IgG1 anti-CD20 were given 10 mg/kg twice weekly for a total of 6
doses. At day 24, antigen challenge with tetanus toxoid fragment C
was given, and mice were sacrificed at days 31 and 38. Tetanus
toxoid (TT) specific antibody production was examined. The results
of this experiment are shown in FIG. 28B. The data shows that
before the antigen challenge, the level of anti-TT specific
antibody was very low in all the groups. After immunization, the
untreated PBS control group showed the highest level of anti-TT
specific antibody level. In comparison, the B cell depleting
anti-CD20 antibody produced low level of antigen specific antibody
level. After immunization, the anti-CD19 S267E/L328F group showed
the lowest level of antigen specific antibodies, whereas the
anti-CD19 IgG1 WT produced a higher level of antigen specific
antibody. These in vivo data show that the anti-CD19 antibody with
enhanced Fc.gamma.RIIb affinity is capable of inhibiting B cell
activation and immunoglobulin secreting plasma cell
differentiation, and thus support the potential of the
immunoglobulins disclosed herein for treating autoimmune and
inflammatory disorders.
Example 9. Co-Engagement of Fc.gamma.RIIb and Other Target
Antigens
[0344] The use of antibodies to coengage CD19 and Fc.gamma.RIIb is
an example of how simultaneous high affinity engagement of a B cell
antigen and Fc.gamma.RIIb may be used to inhibit activation or
proliferation of Fc.gamma.RIIb+ cells. As discussed above,
Fc.gamma.RIIb is a negative regulator of a number of cell types,
including but not limited to B cells, plasma cells, monocytes,
macrophages, dendritic cells, neutrophils, basophils, eosinophils,
and mast cells. A variety of antigens expressed on these
Fc.gamma.RIIb+ cell types may be also be co-targeted with high
affinity Fc.gamma.RIIb binding to inhibit cellular activation
and/or proliferation. FIG. 29 provides a number of examples of
antigens and cell types that may be targeted by the immunoglobulins
disclosed herein.
[0345] At the outset, it is not clear which antigens may serve as
effective co-targets with Fc.gamma.RIIb for modulation of cellular
activity. A likely key aspect of a potential co-target is its
functional role in the cell, and in particular whether its
intracellular signaling pathways (if any) overlap with those of
Fc.gamma.RIIb. CD19 is a co-receptor of the BCR complex, and thus
the capacity of high affinity co-engagement of CD19 and
Fc.gamma.RIIb to inhibit B cell activation is likely related to the
association of CD19 with BCR and the negative regulatory role of
Fc.gamma.RIIb in inhibiting BCR-stimulated activation. Importantly,
however, CD19 is not involved in antigen recognition, which is the
specific function of the (IgM) component of the BCR. Rather CD19,
and other proteins such as CD21, CD22, CD72, CD81, and Leu13, are
BCR co-receptors. Of course, targeting of other components of the
BCR, including the antigen recognition domain (, also referred to
as IgM), and the signaling domains CD79a (Ig.alpha.) and CD79b
(Ig(3), is also supported by the data herein. However, given the
complex biochemical pathways involved in regulating cellular
activation and proliferation of these cell types, evaluating which
antigens (FIG. 29) may serve as effective co-targets with
Fc.gamma.RIIb for modulation of cellular activity requires
experimentation.
[0346] In order to evaluate which antigens may be effective
co-targets with Fc.gamma.RIIb for modulating cellular activity, the
S267E/L328F (high Fc.gamma.RIIb affinity) variant, along with WT
IgG1 and Fc-KO variant(s) ( 236R/L328R and/or G236R/L328R) were
cloned into antibodies specific for a variety of other antigens
expressed on Fc.gamma.RIIb+ cells, including CD19, CD20, CD22,
CD23, CD40, CD52, and CD79b. In several cases, multiple Fv's
targeting the same antigen were constructed in order to assess the
epitope-dependence of the effects. FIG. 54A-FIG. 54D list the heavy
and light chain variable regions (VH and VL) of the antibodies
used. The VH and VL genes targeting these antigens were constructed
by gene synthesis, and variants were constructed, expressed, and
purified as described above.
[0347] The effect of high affinity co-engagement of these antigens
with Fc.gamma.RIIb was evaluated using the ATP-dependent
luminescence B cell viability assay as described above. FIGS. 30
-35 show the results of these experiments. The data indicate that
CD79b is also an effective co-target for using high affinity
Fc.gamma.RIIb co-engagement to inhibit B cell activation. This is
consistent with its role as the signaling component of the BCR
complex. Results using two additional anti-CD19 antibodies again
confirmed the amenability of this antigen to controlling B cell
activation using high affinity Fc.gamma.RIIb co-ligation,
irrespective of the specific epitope targeted. In contrast, no
effect of high affinity Fc.gamma.RIIb co-engagement was observed
for antibodies with specificity for CD20, CD23, and CD52.
Unexpectedly, dual targeting of Fc.gamma.RIIb using antibodies
having specificity for CD22 and CD40 resulted in enhanced B cell
activation. In the case of CD22, this may be the result of its role
as a negative regulator of BCR activation. In the case of CD40,
this may be the result of its role as a positive regulator of B
cell activation via engagement at the T cell interface. It is known
that some of the antibodies used are agonist, that is to say that
their binding of CD40 on B cells and other cells promotes positive
signaling and activation of B cells. In a sense these antibodies
are mimicking the co-activation signal of a T cell. The antibody
(and thus epitope) dependence of this activation is likely related
to the capacity of the antibodies to agonize. The reason for the
enhanced agonism and stimulation of the B cells upon high affinity
(S267E/L328F) engagement of Fc.gamma.RIIb, but not using WT IgG1 or
Fc-KO, is not currently clear, and requires further study.
[0348] Select antibodies targeting other antigens were tested
further for their capacity to inhibit intracellular calcium
mobilization using the assay described above. The results in FIG.
36 agree well with the data from the B cell viability assay.
Whereas high affinity co-ligation of Fc.gamma.RIIb and CD23 had no
effect on calcium mobilization, CD79b is an effective co-target for
inhibition of calcium. High affinity Fc.gamma.RIIb co-ligation with
CD22 and CD40 resulted in an increase in calcium mobilization,
again consistent with the viability results.
[0349] In order to screen a larger set of antigens using commercial
reagents, a novel method was developed for evaluating the capacity
of Fc.gamma.RIIb/antigen co-engagement to inhibit of cellular
activity. This approach uses a haptenized version of an antibody or
ligand that has specificity for the target antigen, together with
variant versions of an anti-hapten antibody. This concept is
illustrated in FIG. 37. A variety of haptens are known in the art
that may be used for this approach, including but not limited to
FITC, biotin, and nitrophenyl.
[0350] The VH and VL genes of the anti-FITC antibody 4-4-20 were
constructed by gene synthesis, and variants were constructed with
enhanced affinity for Fc.gamma.RIIb (S267E/L328F), along with WT
IgG1 and Fc.gamma.R knockout variant(s) ( 236R/L328R and/or
G236R/L328F). Antibodies were constructed, expressed and purified
as described above. Commercial antibodies targeting antigens mu
(.mu.), CD19, CD20, CD21, CD24, CD35, CD45RA, CD72, CD79a, CD79b,
CD80, CD81, CD86, and HLA-DR were purchased from Beckman Coulter
(Fullerton, Calif.), BD Pharmingen (San Jose, Calif.), AbD Serotec
(Raleigh, N.C.), or GenTex, Inc. (San Antonio, Tex.). FITC labeling
reagent (Pierce Biotech, Inc., Rockford, III.) was used to label
commercial antibodies according to the supplied protocol at either
room temperature or 37.degree. C. for 1 hour. After labeling,
un-reacted label was removed using BioSpin P-6 or P-30 columns from
BioRad (Hercules, Calif.) and used with varying concentrations of
anti-FITC antibodies in proliferation experiments as described
above.
[0351] The effectiveness of the hapten approach for screening
antigens was first confirmed using anti-.mu. and anti-CD19
antibodies, two antigens that are known to mediate inhibitory
activity upon high affinity co-engagement with Fc.gamma.RIIb. FIGS.
38 and 39 show anti-FITC antibody variants with high affinity for
Fc.gamma.RIIb, but not WT IgG1 or Fc-KO variants, are able to
inhibit B cell activation in the presence of FITC-labeled anti-mu
and anti-CD19 antibodies. These data are consistent with the above
approach wherein variants were incorporated directly into the
antibody with specificity for CD19 or mu, and thus confirm the use
of the hapten approach for screening target antigens for capacity
to modulate cellular activity upon high affinity co-engagement with
Fc.gamma.RIIb.
[0352] FIGS. 40-52 show data from the ATP-dependent luminescence B
cell viability using Fc variant versions of anti-FITC antibodies
and antibodies targeting CD20, CD21, CD24, CD35, CD45RA, CD72,
CD79a, CD79b, CD80, CD81, CD86, and HLA-DR. Inhibitory activity was
observed for targeting of CD79a and CD79b, consistent with their
role in BCR signaling.
[0353] Targeting of CD81 and HLA-DR resulted in possible
inhibition. The role of CD81 as a BCR co-receptor would seem to
support the result for this antigen. The amenability of these
antigens as co-targets for controlling cellular activation using
high affinity Fc.gamma.RIIb binding requires further study.
Stimulatory activity was observed for co-targeting of CD72 with
high affinity Fc.gamma.RIIb affinity.
[0354] FIG. 53 provides a summary of the results from the target
antigen screening by both the Fc variant and hapten approaches. The
data indicate that immunoglobulins that coengage with high affinity
both Fc.gamma.RIIb and .mu., CD19, CD79a, Cd79b, CD81, and HLA-DR
have potential for inhibiting the activation of Fc.gamma.RIIb+
cells. The data also indicate that immunoglobulins that coengage
with high affinity both Fc.gamma.RIIb and CD22, CD40, and CD72 have
potential for stimulating Fc.gamma.RIIb+ cells. Overall, the
results of this work suggest that simultaneous high affinity
engagement of Fc.gamma.RIIb and antigens involved or associated
with the BCR complex, including .mu., CD79a, CD79b, CD19, CD21,
CD22, CD72, CD81, and Leu13, are methods for controlling the
activation, proliferation, and/or viability of B cells.
[0355] All cited references are herein expressly incorporated by
reference in their entirety.
[0356] Whereas particular embodiments have been described above for
purposes of illustration, it will be appreciated by those skilled
in the art that numerous variations of the details may be made
without departing from the invention as described in the appended
claims.
Sequence CWU 1 SEQUENCE LISTING <160> NUMBER OF SEQ ID
NOS: 40 <210> SEQ ID NO 1 <211> LENGTH: 112 <212>
TYPE: PRT <213> ORGANISM: Artificial Sequence <220>
FEATURE: <223> OTHER INFORMATION: Synthesized HuAM4G7 VL
<400> SEQUENCE: 1 Asp Ile Val Met Thr Gln Ser Pro Ala Thr Leu
Ser Leu Ser Pro Gly 1 5 10 15 Glu Arg Ala Thr Leu Ser Cys Arg Ser
Ser Lys Ser Leu Gln Asn Val 20 25 30 Asn Gly Asn Thr Tyr Leu Tyr
Trp Phe Gln Gln Lys Pro Gly Gln Ser 35 40 45 Pro Gln Leu Leu Ile
Tyr Arg Met Ser Asn Leu Asn Ser Gly Val Pro 50 55 60 Asp Arg Phe
Ser Gly Ser Gly Ser Gly Thr Glu Phe Thr Leu Thr Ile 65 70 75 80 Ser
Ser Leu Glu Pro Glu Asp Phe Ala Val Tyr Tyr Cys Met Gln His 85 90
95 Leu Glu Tyr Pro Ile Thr Phe Gly Ala Gly Thr Lys Leu Glu Ile Lys
100 105 110 <210> SEQ ID NO 2 <211> LENGTH: 121
<212> TYPE: PRT <213> ORGANISM: Artificial Sequence
<220> FEATURE: <223> OTHER INFORMATION: Synthesized
HuAM4G7 VH <400> SEQUENCE: 2 Glu Val Gln Leu Val Glu Ser Gly
Gly Gly Leu Val Lys Pro Gly Gly 1 5 10 15 Ser Leu Lys Leu Ser Cys
Ala Ala Ser Gly Tyr Thr Phe Thr Ser Tyr 20 25 30 Val Met His Trp
Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Ile 35 40 45 Gly Tyr
Ile Asn Pro Tyr Asn Asp Gly Thr Lys Tyr Asn Glu Lys Phe 50 55 60
Gln Gly Arg Val Thr Ile Ser Ser Asp Lys Ser Ile Ser Thr Ala Tyr 65
70 75 80 Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Met Tyr
Tyr Cys 85 90 95 Ala Arg Gly Thr Tyr Tyr Tyr Gly Thr Arg Val Phe
Asp Tyr Trp Gly 100 105 110 Gln Gly Thr Leu Val Thr Val Ser Ser 115
120 <210> SEQ ID NO 3 <211> LENGTH: 107 <212>
TYPE: PRT <213> ORGANISM: Homo sapiens <400> SEQUENCE:
3 Arg Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu 1
5 10 15 Gln Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn
Phe 20 25 30 Tyr Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn
Ala Leu Gln 35 40 45 Ser Gly Asn Ser Gln Glu Ser Val Thr Glu Gln
Asp Ser Lys Asp Ser 50 55 60 Thr Tyr Ser Leu Ser Ser Thr Leu Thr
Leu Ser Lys Ala Asp Tyr Glu 65 70 75 80 Lys His Lys Val Tyr Ala Cys
Glu Val Thr His Gln Gly Leu Ser Ser 85 90 95 Pro Val Thr Lys Ser
Phe Asn Arg Gly Glu Cys 100 105 <210> SEQ ID NO 4 <211>
LENGTH: 330 <212> TYPE: PRT <213> ORGANISM: Homo
sapiens <400> SEQUENCE: 4 Ala Ser Thr Lys Gly Pro Ser Val Phe
Pro Leu Ala Pro Ser Ser Lys 1 5 10 15 Ser Thr Ser Gly Gly Thr Ala
Ala Leu Gly Cys Leu Val Lys Asp Tyr 20 25 30 Phe Pro Glu Pro Val
Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 35 40 45 Gly Val His
Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 50 55 60 Leu
Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr 65 70
75 80 Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp
Lys 85 90 95 Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys
Pro Pro Cys 100 105 110 Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val
Phe Leu Phe Pro Pro 115 120 125 Lys Pro Lys Asp Thr Leu Met Ile Ser
Arg Thr Pro Glu Val Thr Cys 130 135 140 Val Val Val Asp Val Ser His
Glu Asp Pro Glu Val Lys Phe Asn Trp 145 150 155 160 Tyr Val Asp Gly
Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu 165 170 175 Glu Gln
Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu 180 185 190
His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn 195
200 205 Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys
Gly 210 215 220 Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser
Arg Glu Glu 225 230 235 240 Met Thr Lys Asn Gln Val Ser Leu Thr Cys
Leu Val Lys Gly Phe Tyr 245 250 255 Pro Ser Asp Ile Ala Val Glu Trp
Glu Ser Asn Gly Gln Pro Glu Asn 260 265 270 Asn Tyr Lys Thr Thr Pro
Pro Val Leu Asp Ser Asp Gly Ser Phe Phe 275 280 285 Leu Tyr Ser Lys
Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn 290 295 300 Val Phe
Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr 305 310 315
320 Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 325 330 <210> SEQ
ID NO 5 <211> LENGTH: 330 <212> TYPE: PRT <213>
ORGANISM: Artificial Sequence <220> FEATURE: <223>
OTHER INFORMATION: Synthesized S267E/L328F IgG1 constant chain
<400> SEQUENCE: 5 Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu
Ala Pro Ser Ser Lys 1 5 10 15 Ser Thr Ser Gly Gly Thr Ala Ala Leu
Gly Cys Leu Val Lys Asp Tyr 20 25 30 Phe Pro Glu Pro Val Thr Val
Ser Trp Asn Ser Gly Ala Leu Thr Ser 35 40 45 Gly Val His Thr Phe
Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 50 55 60 Leu Ser Ser
Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr 65 70 75 80 Tyr
Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys 85 90
95 Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys
100 105 110 Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe
Pro Pro 115 120 125 Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro
Glu Val Thr Cys 130 135 140 Val Val Val Asp Val Glu His Glu Asp Pro
Glu Val Lys Phe Asn Trp 145 150 155 160 Tyr Val Asp Gly Val Glu Val
His Asn Ala Lys Thr Lys Pro Arg Glu 165 170 175 Glu Gln Tyr Asn Ser
Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu 180 185 190 His Gln Asp
Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn 195 200 205 Lys
Ala Phe Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly 210 215
220 Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu
225 230 235 240 Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys
Gly Phe Tyr 245 250 255 Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn
Gly Gln Pro Glu Asn 260 265 270 Asn Tyr Lys Thr Thr Pro Pro Val Leu
Asp Ser Asp Gly Ser Phe Phe 275 280 285 Leu Tyr Ser Lys Leu Thr Val
Asp Lys Ser Arg Trp Gln Gln Gly Asn 290 295 300 Val Phe Ser Cys Ser
Val Met His Glu Ala Leu His Asn His Tyr Thr 305 310 315 320 Gln Lys
Ser Leu Ser Leu Ser Pro Gly Lys 325 330 <210> SEQ ID NO 6
<211> LENGTH: 330 <212> TYPE: PRT <213> ORGANISM:
Artificial Sequence <220> FEATURE: <223> OTHER
INFORMATION: Synthesized G236D/S267E IgG1 constant chain
<400> SEQUENCE: 6 Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu
Ala Pro Ser Ser Lys 1 5 10 15 Ser Thr Ser Gly Gly Thr Ala Ala Leu
Gly Cys Leu Val Lys Asp Tyr 20 25 30 Phe Pro Glu Pro Val Thr Val
Ser Trp Asn Ser Gly Ala Leu Thr Ser 35 40 45 Gly Val His Thr Phe
Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 50 55 60 Leu Ser Ser
Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr 65 70 75 80 Tyr
Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys 85 90
95 Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys
100 105 110 Pro Ala Pro Glu Leu Leu Asp Gly Pro Ser Val Phe Leu Phe
Pro Pro 115 120 125 Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro
Glu Val Thr Cys 130 135 140 Val Val Val Asp Val Glu His Glu Asp Pro
Glu Val Lys Phe Asn Trp 145 150 155 160 Tyr Val Asp Gly Val Glu Val
His Asn Ala Lys Thr Lys Pro Arg Glu 165 170 175 Glu Gln Tyr Asn Ser
Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu 180 185 190 His Gln Asp
Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn 195 200 205 Lys
Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly 210 215
220 Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu
225 230 235 240 Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys
Gly Phe Tyr 245 250 255 Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn
Gly Gln Pro Glu Asn 260 265 270 Asn Tyr Lys Thr Thr Pro Pro Val Leu
Asp Ser Asp Gly Ser Phe Phe 275 280 285 Leu Tyr Ser Lys Leu Thr Val
Asp Lys Ser Arg Trp Gln Gln Gly Asn 290 295 300 Val Phe Ser Cys Ser
Val Met His Glu Ala Leu His Asn His Tyr Thr 305 310 315 320 Gln Lys
Ser Leu Ser Leu Ser Pro Gly Lys 325 330 <210> SEQ ID NO 7
<211> LENGTH: 219 <212> TYPE: PRT <213> ORGANISM:
Artificial Sequence <220> FEATURE: <223> OTHER
INFORMATION: Synthesized HuAM4G7 light chain (VH-C ) <400>
SEQUENCE: 7 Asp Ile Val Met Thr Gln Ser Pro Ala Thr Leu Ser Leu Ser
Pro Gly 1 5 10 15 Glu Arg Ala Thr Leu Ser Cys Arg Ser Ser Lys Ser
Leu Gln Asn Val 20 25 30 Asn Gly Asn Thr Tyr Leu Tyr Trp Phe Gln
Gln Lys Pro Gly Gln Ser 35 40 45 Pro Gln Leu Leu Ile Tyr Arg Met
Ser Asn Leu Asn Ser Gly Val Pro 50 55 60 Asp Arg Phe Ser Gly Ser
Gly Ser Gly Thr Glu Phe Thr Leu Thr Ile 65 70 75 80 Ser Ser Leu Glu
Pro Glu Asp Phe Ala Val Tyr Tyr Cys Met Gln His 85 90 95 Leu Glu
Tyr Pro Ile Thr Phe Gly Ala Gly Thr Lys Leu Glu Ile Lys 100 105 110
Arg Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu 115
120 125 Gln Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn
Phe 130 135 140 Tyr Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn
Ala Leu Gln 145 150 155 160 Ser Gly Asn Ser Gln Glu Ser Val Thr Glu
Gln Asp Ser Lys Asp Ser 165 170 175 Thr Tyr Ser Leu Ser Ser Thr Leu
Thr Leu Ser Lys Ala Asp Tyr Glu 180 185 190 Lys His Lys Val Tyr Ala
Cys Glu Val Thr His Gln Gly Leu Ser Ser 195 200 205 Pro Val Thr Lys
Ser Phe Asn Arg Gly Glu Cys 210 215 <210> SEQ ID NO 8
<211> LENGTH: 451 <212> TYPE: PRT <213> ORGANISM:
Artificial Sequence <220> FEATURE: <223> OTHER
INFORMATION: Synthesized HuAM4G7 IgG1 heavy chain <400>
SEQUENCE: 8 Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Lys Pro
Gly Gly 1 5 10 15 Ser Leu Lys Leu Ser Cys Ala Ala Ser Gly Tyr Thr
Phe Thr Ser Tyr 20 25 30 Val Met His Trp Val Arg Gln Ala Pro Gly
Lys Gly Leu Glu Trp Ile 35 40 45 Gly Tyr Ile Asn Pro Tyr Asn Asp
Gly Thr Lys Tyr Asn Glu Lys Phe 50 55 60 Gln Gly Arg Val Thr Ile
Ser Ser Asp Lys Ser Ile Ser Thr Ala Tyr 65 70 75 80 Met Glu Leu Ser
Ser Leu Arg Ser Glu Asp Thr Ala Met Tyr Tyr Cys 85 90 95 Ala Arg
Gly Thr Tyr Tyr Tyr Gly Thr Arg Val Phe Asp Tyr Trp Gly 100 105 110
Gln Gly Thr Leu Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser 115
120 125 Val Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr
Ala 130 135 140 Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro
Val Thr Val 145 150 155 160 Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly
Val His Thr Phe Pro Ala 165 170 175 Val Leu Gln Ser Ser Gly Leu Tyr
Ser Leu Ser Ser Val Val Thr Val 180 185 190 Pro Ser Ser Ser Leu Gly
Thr Gln Thr Tyr Ile Cys Asn Val Asn His 195 200 205 Lys Pro Ser Asn
Thr Lys Val Asp Lys Lys Val Glu Pro Lys Ser Cys 210 215 220 Asp Lys
Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly 225 230 235
240 Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met
245 250 255 Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val
Ser His 260 265 270 Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp
Gly Val Glu Val 275 280 285 His Asn Ala Lys Thr Lys Pro Arg Glu Glu
Gln Tyr Asn Ser Thr Tyr 290 295 300 Arg Val Val Ser Val Leu Thr Val
Leu His Gln Asp Trp Leu Asn Gly 305 310 315 320 Lys Glu Tyr Lys Cys
Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile 325 330 335 Glu Lys Thr
Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val 340 345 350 Tyr
Thr Leu Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser 355 360
365 Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu
370 375 380 Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr
Pro Pro 385 390 395 400 Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr
Ser Lys Leu Thr Val 405 410 415 Asp Lys Ser Arg Trp Gln Gln Gly Asn
Val Phe Ser Cys Ser Val Met 420 425 430 His Glu Ala Leu His Asn His
Tyr Thr Gln Lys Ser Leu Ser Leu Ser 435 440 445 Pro Gly Lys 450
<210> SEQ ID NO 9 <211> LENGTH: 451 <212> TYPE:
PRT <213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: Synthesized HuAM4G7 S267E/L328F
heavy chain <400> SEQUENCE: 9 Glu Val Gln Leu Val Glu Ser Gly
Gly Gly Leu Val Lys Pro Gly Gly 1 5 10 15 Ser Leu Lys Leu Ser Cys
Ala Ala Ser Gly Tyr Thr Phe Thr Ser Tyr 20 25 30 Val Met His Trp
Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Ile 35 40 45 Gly Tyr
Ile Asn Pro Tyr Asn Asp Gly Thr Lys Tyr Asn Glu Lys Phe 50 55 60
Gln Gly Arg Val Thr Ile Ser Ser Asp Lys Ser Ile Ser Thr Ala Tyr 65
70 75 80 Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Met Tyr
Tyr Cys 85 90 95 Ala Arg Gly Thr Tyr Tyr Tyr Gly Thr Arg Val Phe
Asp Tyr Trp Gly 100 105 110 Gln Gly Thr Leu Val Thr Val Ser Ser Ala
Ser Thr Lys Gly Pro Ser 115 120 125 Val Phe Pro Leu Ala Pro Ser Ser
Lys Ser Thr Ser Gly Gly Thr Ala 130 135 140 Ala Leu Gly Cys Leu Val
Lys Asp Tyr Phe Pro Glu Pro Val Thr Val 145 150 155 160 Ser Trp Asn
Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala 165 170 175 Val
Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val 180 185
190 Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His
195 200 205 Lys Pro Ser Asn Thr Lys Val Asp Lys Lys Val Glu Pro Lys
Ser Cys 210 215 220 Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro
Glu Leu Leu Gly 225 230 235 240 Gly Pro Ser Val Phe Leu Phe Pro Pro
Lys Pro Lys Asp Thr Leu Met 245 250 255 Ile Ser Arg Thr Pro Glu Val
Thr Cys Val Val Val Asp Val Glu His 260 265 270 Glu Asp Pro Glu Val
Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val 275 280 285 His Asn Ala
Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr 290 295 300 Arg
Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly 305 310
315 320 Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Phe Pro Ala Pro
Ile 325 330 335 Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu
Pro Gln Val 340 345 350 Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Thr
Lys Asn Gln Val Ser 355 360 365 Leu Thr Cys Leu Val Lys Gly Phe Tyr
Pro Ser Asp Ile Ala Val Glu 370 375 380 Trp Glu Ser Asn Gly Gln Pro
Glu Asn Asn Tyr Lys Thr Thr Pro Pro 385 390 395 400 Val Leu Asp Ser
Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val 405 410 415 Asp Lys
Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met 420 425 430
His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser 435
440 445 Pro Gly Lys 450 <210> SEQ ID NO 10 <211>
LENGTH: 451 <212> TYPE: PRT <213> ORGANISM: Artificial
Sequence <220> FEATURE: <223> OTHER INFORMATION:
Synthesized HuAM4G7 G236D/S267E heavy chain <400> SEQUENCE:
10 Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Lys Pro Gly Gly
1 5 10 15 Ser Leu Lys Leu Ser Cys Ala Ala Ser Gly Tyr Thr Phe Thr
Ser Tyr 20 25 30 Val Met His Trp Val Arg Gln Ala Pro Gly Lys Gly
Leu Glu Trp Ile 35 40 45 Gly Tyr Ile Asn Pro Tyr Asn Asp Gly Thr
Lys Tyr Asn Glu Lys Phe 50 55 60 Gln Gly Arg Val Thr Ile Ser Ser
Asp Lys Ser Ile Ser Thr Ala Tyr 65 70 75 80 Met Glu Leu Ser Ser Leu
Arg Ser Glu Asp Thr Ala Met Tyr Tyr Cys 85 90 95 Ala Arg Gly Thr
Tyr Tyr Tyr Gly Thr Arg Val Phe Asp Tyr Trp Gly 100 105 110 Gln Gly
Thr Leu Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser 115 120 125
Val Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala 130
135 140 Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr
Val 145 150 155 160 Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His
Thr Phe Pro Ala 165 170 175 Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu
Ser Ser Val Val Thr Val 180 185 190 Pro Ser Ser Ser Leu Gly Thr Gln
Thr Tyr Ile Cys Asn Val Asn His 195 200 205 Lys Pro Ser Asn Thr Lys
Val Asp Lys Lys Val Glu Pro Lys Ser Cys 210 215 220 Asp Lys Thr His
Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Asp 225 230 235 240 Gly
Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met 245 250
255 Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Glu His
260 265 270 Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val
Glu Val 275 280 285 His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr
Asn Ser Thr Tyr 290 295 300 Arg Val Val Ser Val Leu Thr Val Leu His
Gln Asp Trp Leu Asn Gly 305 310 315 320 Lys Glu Tyr Lys Cys Lys Val
Ser Asn Lys Ala Leu Pro Ala Pro Ile 325 330 335 Glu Lys Thr Ile Ser
Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val 340 345 350 Tyr Thr Leu
Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser 355 360 365 Leu
Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu 370 375
380 Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro
385 390 395 400 Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys
Leu Thr Val 405 410 415 Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe
Ser Cys Ser Val Met 420 425 430 His Glu Ala Leu His Asn His Tyr Thr
Gln Lys Ser Leu Ser Leu Ser 435 440 445 Pro Gly Lys 450 <210>
SEQ ID NO 11 <211> LENGTH: 106 <212> TYPE: PRT
<213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: Synthesized anti-RSV Numax VL
<400> SEQUENCE: 11 Asp Ile Gln Met Thr Gln Ser Pro Ser Thr
Leu Ser Ala Ser Val Gly 1 5 10 15 Asp Arg Val Thr Ile Thr Cys Ser
Ala Ser Ser Arg Val Gly Tyr Met 20 25 30 His Trp Tyr Gln Gln Lys
Pro Gly Lys Ala Pro Lys Leu Leu Ile Tyr 35 40 45 Asp Thr Ser Lys
Leu Ala Ser Gly Val Pro Ser Arg Phe Ser Gly Ser 50 55 60 Gly Ser
Gly Thr Glu Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro Asp 65 70 75 80
Asp Phe Ala Thr Tyr Tyr Cys Phe Gln Gly Ser Gly Tyr Pro Phe Thr 85
90 95 Phe Gly Gly Gly Thr Lys Val Glu Ile Lys 100 105 <210>
SEQ ID NO 12 <211> LENGTH: 120 <212> TYPE: PRT
<213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: Synthesized anti-RSV Numax VH
<400> SEQUENCE: 12 Gln Val Thr Leu Arg Glu Ser Gly Pro Ala
Leu Val Lys Pro Thr Gln 1 5 10 15 Thr Leu Thr Leu Thr Cys Thr Phe
Ser Gly Phe Ser Leu Ser Thr Ala 20 25 30 Gly Met Ser Val Gly Trp
Ile Arg Gln Pro Pro Gly Lys Ala Leu Glu 35 40 45 Trp Leu Ala Asp
Ile Trp Trp Asp Asp Lys Lys His Tyr Asn Pro Ser 50 55 60 Leu Lys
Asp Arg Leu Thr Ile Ser Lys Asp Thr Ser Lys Asn Gln Val 65 70 75 80
Val Leu Lys Val Thr Asn Met Asp Pro Ala Asp Thr Ala Thr Tyr Tyr 85
90 95 Cys Ala Arg Asp Met Ile Phe Asn Phe Tyr Phe Asp Val Trp Gly
Gln 100 105 110 Gly Thr Thr Val Thr Val Ser Ser 115 120 <210>
SEQ ID NO 13 <211> LENGTH: 112 <212> TYPE: PRT
<213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: Synthesized anti-FITC VL <400>
SEQUENCE: 13 Asp Val Val Met Thr Gln Thr Pro Leu Ser Leu Pro Val
Ser Leu Gly 1 5 10 15 Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln
Ser Leu Val His Ser 20 25 30 Asn Gly Asn Thr Tyr Leu Arg Trp Tyr
Leu Gln Lys Pro Gly Gln Ser 35 40 45 Pro Lys Val Leu Ile Tyr Lys
Val Ser Asn Arg Phe Ser Gly Val Pro 50 55 60 Asp Arg Phe Ser Gly
Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile 65 70 75 80 Ser Arg Val
Glu Ala Glu Asp Leu Gly Val Tyr Phe Cys Ser Gln Ser 85 90 95 Thr
His Val Pro Trp Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys 100 105
110 <210> SEQ ID NO 14 <211> LENGTH: 118 <212>
TYPE: PRT <213> ORGANISM: Artificial Sequence <220>
FEATURE: <223> OTHER INFORMATION: Synthesized anti-FITC VH
<400> SEQUENCE: 14 Glu Val Lys Leu Asp Glu Thr Gly Gly Gly
Leu Val Gln Pro Gly Arg 1 5 10 15 Pro Met Lys Leu Ser Cys Val Ala
Ser Gly Phe Thr Phe Ser Asp Tyr 20 25 30 Trp Met Asn Trp Val Arg
Gln Ser Pro Glu Lys Gly Leu Glu Trp Val 35 40 45 Ala Gln Ile Arg
Asn Lys Pro Tyr Asn Tyr Glu Thr Tyr Tyr Ser Asp 50 55 60 Ser Val
Lys Gly Arg Phe Thr Ile Ser Arg Asp Asp Ser Lys Ser Ser 65 70 75 80
Val Tyr Leu Gln Met Asn Asn Leu Arg Val Glu Asp Met Gly Ile Tyr 85
90 95 Tyr Cys Thr Gly Ser Tyr Tyr Gly Met Asp Tyr Trp Gly Gln Gly
Thr 100 105 110 Ser Val Thr Val Ser Ser 115 <210> SEQ ID NO
15 <211> LENGTH: 111 <212> TYPE: PRT <213>
ORGANISM: Mus musculus <400> SEQUENCE: 15 Asp Ile Val Leu Thr
Gln Ser Pro Ala Ser Leu Ala Val Ser Leu Gly 1 5 10 15 Gln Arg Ala
Thr Ile Ser Cys Lys Ala Ser Gln Ser Val Asp Tyr Asp 20 25 30 Gly
Asp Ser Phe Leu Asn Trp Tyr Gln Gln Lys Pro Gly Gln Pro Pro 35 40
45 Lys Leu Phe Ile Tyr Ala Ala Ser Asn Leu Glu Ser Gly Ile Pro Ala
50 55 60 Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Asn
Ile His 65 70 75 80 Pro Val Glu Glu Glu Asp Ala Ala Thr Tyr Tyr Cys
Gln Gln Ser Asn 85 90 95 Glu Asp Pro Leu Thr Phe Gly Ala Gly Thr
Glu Leu Glu Leu Lys 100 105 110 <210> SEQ ID NO 16
<211> LENGTH: 117 <212> TYPE: PRT <213> ORGANISM:
Mus musculus <400> SEQUENCE: 16 Glu Val Gln Leu Gln Gln Ser
Gly Ala Glu Leu Met Lys Pro Gly Ala 1 5 10 15 Ser Val Lys Ile Ser
Cys Lys Ala Thr Gly Tyr Thr Phe Ser Ser Tyr 20 25 30 Trp Ile Glu
Trp Val Lys Gln Arg Pro Gly His Gly Leu Glu Trp Ile 35 40 45 Gly
Glu Ile Leu Pro Gly Gly Gly Asp Thr Asn Tyr Asn Glu Ile Phe 50 55
60 Lys Gly Lys Ala Thr Phe Thr Ala Asp Thr Ser Ser Asn Thr Ala Tyr
65 70 75 80 Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr
Tyr Cys 85 90 95 Thr Arg Arg Val Pro Val Tyr Phe Asp Tyr Trp Gly
Gln Gly Thr Ser 100 105 110 Val Thr Val Ser Ser 115 <210> SEQ
ID NO 17 <211> LENGTH: 106 <212> TYPE: PRT <213>
ORGANISM: Artificial Sequence <220> FEATURE: <223>
OTHER INFORMATION: Synthesized anti-CD20 Pro70769 VL <400>
SEQUENCE: 17 Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala
Ser Val Gly 1 5 10 15 Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Ser
Ser Val Ser Tyr Met 20 25 30 His Trp Tyr Gln Gln Lys Pro Gly Lys
Ala Pro Lys Pro Leu Ile Tyr 35 40 45 Ala Pro Ser Asn Leu Ala Ser
Gly Val Pro Ser Arg Phe Ser Gly Ser 50 55 60 Gly Ser Gly Thr Asp
Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro Glu 65 70 75 80 Asp Phe Ala
Thr Tyr Tyr Cys Gln Gln Trp Ser Phe Asn Pro Pro Thr 85 90 95 Phe
Gly Gln Gly Thr Lys Val Glu Ile Lys 100 105 <210> SEQ ID NO
18 <211> LENGTH: 122 <212> TYPE: PRT <213>
ORGANISM: Artificial Sequence <220> FEATURE: <223>
OTHER INFORMATION: Synthesized anti-CD20 Pro70769 VH <400>
SEQUENCE: 18 Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln
Pro Gly Gly 1 5 10 15 Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Tyr
Thr Phe Thr Ser Tyr 20 25 30 Asn Met His Trp Val Arg Gln Ala Pro
Gly Lys Gly Leu Glu Trp Val 35 40 45 Gly Ala Ile Tyr Pro Gly Asn
Gly Asp Thr Ser Tyr Asn Gln Lys Phe 50 55 60 Lys Gly Arg Phe Thr
Ile Ser Val Asp Lys Ser Lys Asn Thr Leu Tyr 65 70 75 80 Leu Gln Met
Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 85 90 95 Ala
Arg Val Val Tyr Tyr Ser Asn Ser Tyr Trp Tyr Phe Asp Val Trp 100 105
110 Gly Gln Gly Thr Leu Val Thr Val Ser Ser 115 120 <210> SEQ
ID NO 19 <211> LENGTH: 107 <212> TYPE: PRT <213>
ORGANISM: Artificial Sequence <220> FEATURE: <223>
OTHER INFORMATION: Synthesized anti-CD52 Campath VL <400>
SEQUENCE: 19 Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala
Ser Val Gly 1 5 10 15 Asp Arg Val Thr Ile Thr Cys Lys Ala Ser Gln
Asn Ile Asp Lys Tyr 20 25 30 Leu Asn Trp Tyr Gln Gln Lys Pro Gly
Lys Ala Pro Lys Leu Leu Ile 35 40 45 Tyr Asn Thr Asn Asn Leu Gln
Thr Gly Val Pro Ser Arg Phe Ser Gly 50 55 60 Ser Gly Ser Gly Thr
Asp Phe Thr Phe Thr Ile Ser Ser Leu Gln Pro 65 70 75 80 Glu Asp Ile
Ala Thr Tyr Tyr Cys Leu Gln His Ile Ser Arg Pro Arg 85 90 95 Thr
Phe Gly Gln Gly Thr Lys Val Glu Ile Lys 100 105 <210> SEQ ID
NO 20 <211> LENGTH: 121 <212> TYPE: PRT <213>
ORGANISM: Artificial Sequence <220> FEATURE: <223>
OTHER INFORMATION: Synthesized anti-CD52 Campath VH <400>
SEQUENCE: 20 Gln Val Gln Leu Gln Glu Ser Gly Pro Gly Leu Val Arg
Pro Ser Gln 1 5 10 15 Thr Leu Ser Leu Thr Cys Thr Val Ser Gly Phe
Thr Phe Thr Asp Phe 20 25 30 Tyr Met Asn Trp Val Arg Gln Pro Pro
Gly Arg Gly Leu Glu Trp Ile 35 40 45 Gly Phe Ile Arg Asp Lys Ala
Lys Gly Tyr Thr Thr Glu Tyr Asn Pro 50 55 60 Ser Val Lys Gly Arg
Val Thr Met Leu Val Asp Thr Ser Lys Asn Gln 65 70 75 80 Phe Ser Leu
Arg Leu Ser Ser Val Thr Ala Ala Asp Thr Ala Val Tyr 85 90 95 Tyr
Cys Ala Arg Glu Gly His Thr Ala Ala Pro Phe Asp Tyr Trp Gly 100 105
110 Gln Gly Ser Leu Val Thr Val Ser Ser 115 120 <210> SEQ ID
NO 21 <211> LENGTH: 107 <212> TYPE: PRT <213>
ORGANISM: Mus musculus <400> SEQUENCE: 21 Asp Ile Gln Met Thr
Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 1 5 10 15 Asp Arg Val
Thr Ile Thr Cys Arg Ala Ser Gln Asp Ile Arg Tyr Tyr 20 25 30 Leu
Asn Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 35 40
45 Tyr Val Ala Ser Ser Leu Gln Ser Gly Val Pro Ser Arg Phe Ser Gly
50 55 60 Ser Gly Ser Gly Thr Glu Phe Thr Leu Thr Val Ser Ser Leu
Gln Pro 65 70 75 80 Glu Asp Phe Ala Thr Tyr Tyr Cys Leu Gln Val Tyr
Ser Thr Pro Arg 85 90 95 Thr Phe Gly Gln Gly Thr Lys Val Glu Ile
Lys 100 105 <210> SEQ ID NO 22 <211> LENGTH: 118
<212> TYPE: PRT <213> ORGANISM: Mus musculus
<400> SEQUENCE: 22 Glu Val Gln Leu Val Glu Ser Gly Gly Gly
Leu Ala Lys Pro Gly Gly 1 5 10 15 Ser Leu Arg Leu Ser Cys Ala Ala
Ser Gly Phe Arg Phe Thr Phe Asn 20 25 30 Asn Tyr Tyr Met Asp Trp
Val Arg Gln Ala Pro Gly Gln Gly Leu Glu 35 40 45 Trp Val Ser Arg
Ile Ser Ser Ser Gly Asp Pro Thr Trp Tyr Ala Asp 50 55 60 Ser Val
Lys Gly Arg Phe Thr Ile Ser Arg Glu Asn Ala Asn Asn Thr 65 70 75 80
Leu Phe Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr 85
90 95 Tyr Cys Ala Ser Leu Thr Thr Gly Ser Asp Ser Trp Gly Gln Gly
Val 100 105 110 Leu Val Thr Val Ser Ser 115 <210> SEQ ID NO
23 <211> LENGTH: 112 <212> TYPE: PRT <213>
ORGANISM: Mus musculus <400> SEQUENCE: 23 Asp Ile Val Ile Thr
Gln Asp Glu Leu Ser Asn Pro Val Thr Ser Gly 1 5 10 15 Glu Ser Val
Ser Ile Ser Cys Arg Ser Ser Lys Ser Leu Leu Tyr Lys 20 25 30 Asp
Gly Lys Thr Tyr Leu Asn Trp Phe Leu Gln Arg Pro Gly Gln Ser 35 40
45 Pro Gln Leu Leu Met Tyr Leu Met Ser Thr Arg Ala Ser Gly Val Ser
50 55 60 Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu
Glu Ile 65 70 75 80 Ser Arg Val Lys Ala Glu Asp Val Gly Val Tyr Tyr
Cys Gln Gln Leu 85 90 95 Val Glu Tyr Pro Phe Thr Phe Gly Ser Gly
Thr Lys Leu Glu Ile Lys 100 105 110 <210> SEQ ID NO 24
<211> LENGTH: 114 <212> TYPE: PRT <213> ORGANISM:
Mus musculus <400> SEQUENCE: 24 Glu Val Lys Leu Glu Glu Ser
Gly Gly Gly Leu Val Gln Pro Gly Gly 1 5 10 15 Ser Met Lys Leu Ser
Cys Val Ala Ser Gly Phe Thr Phe Ser Gly Tyr 20 25 30 Trp Met Ser
Trp Val Arg Gln Ser Pro Glu Lys Gly Leu Glu Trp Val 35 40 45 Ala
Glu Ile Arg Leu Lys Ser Asp Asn Tyr Ala Thr His Tyr Ala Glu 50 55
60 Ser Val Lys Gly Lys Phe Thr Ile Ser Arg Asp Asp Ser Lys Ser Arg
65 70 75 80 Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Ser Gly
Val Tyr 85 90 95 Tyr Cys Thr Asp Phe Ile Asp Trp Gly Gln Gly Thr
Leu Val Thr Val 100 105 110 Ser Ser <210> SEQ ID NO 25
<211> LENGTH: 107 <212> TYPE: PRT <213> ORGANISM:
Mus musculus <400> SEQUENCE: 25 Asp Ile Gln Met Thr Gln Thr
Thr Ser Ser Leu Ser Ala Ser Leu Gly 1 5 10 15 Asp Arg Val Thr Ile
Ser Cys Arg Ala Ser Gln Asp Ile Ser Asn Tyr 20 25 30 Leu Asn Trp
Tyr Gln Gln Lys Pro Asp Gly Thr Val Lys Leu Leu Ile 35 40 45 Tyr
Tyr Thr Ser Ile Leu His Ser Gly Val Pro Ser Arg Phe Ser Gly 50 55
60 Ser Gly Ser Gly Thr Asp Tyr Ser Leu Thr Ile Ser Asn Leu Glu Gln
65 70 75 80 Glu Asp Phe Ala Thr Tyr Phe Cys Gln Gln Gly Asn Thr Leu
Pro Trp 85 90 95 Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys 100
105 <210> SEQ ID NO 26 <211> LENGTH: 123 <212>
TYPE: PRT <213> ORGANISM: Mus musculus <400> SEQUENCE:
26 Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Lys Pro Gly Gly
1 5 10 15 Ser Leu Lys Leu Ser Cys Ala Ala Ser Gly Phe Ala Phe Ser
Ile Tyr 20 25 30 Asp Met Ser Trp Val Arg Gln Thr Pro Glu Lys Arg
Leu Glu Trp Val 35 40 45 Ala Tyr Ile Ser Ser Gly Gly Gly Thr Thr
Tyr Tyr Pro Asp Thr Val 50 55 60 Lys Gly Arg Phe Thr Ile Ser Arg
Asp Asn Ala Lys Asn Thr Leu Tyr 65 70 75 80 Leu Gln Met Ser Ser Leu
Lys Ser Glu Asp Thr Ala Met Tyr Tyr Cys 85 90 95 Ala Arg His Ser
Gly Tyr Gly Ser Ser Tyr Gly Val Leu Phe Ala Tyr 100 105 110 Trp Gly
Gln Gly Thr Leu Val Thr Thr Ser Ala 115 120 <210> SEQ ID NO
27 <211> LENGTH: 107 <212> TYPE: PRT <213>
ORGANISM: Homo sapiens <400> SEQUENCE: 27 Asp Ile Gln Met Thr
Gln Ser Pro Ser Ser Val Ser Ala Ser Val Gly 1 5 10 15 Asp Arg Val
Thr Ile Thr Cys Arg Ala Ser Gln Gly Ile Tyr Ser Trp 20 25 30 Leu
Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Asn Leu Leu Ile 35 40
45 Tyr Thr Ala Ser Thr Leu Gln Ser Gly Val Pro Ser Arg Phe Ser Gly
50 55 60 Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu
Gln Pro 65 70 75 80 Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Ala Asn
Ile Phe Pro Leu 85 90 95 Thr Phe Gly Gly Gly Thr Lys Val Glu Ile
Lys 100 105 <210> SEQ ID NO 28 <211> LENGTH: 126
<212> TYPE: PRT <213> ORGANISM: Homo sapiens
<400> SEQUENCE: 28 Gln Val Gln Leu Val Gln Ser Gly Ala Glu
Val Lys Lys Pro Gly Ala 1 5 10 15 Ser Val Lys Val Ser Cys Lys Ala
Ser Gly Tyr Thr Phe Thr Gly Tyr 20 25 30 Tyr Met His Trp Val Arg
Gln Ala Pro Gly Gln Gly Leu Glu Trp Met 35 40 45 Gly Trp Ile Asn
Pro Asp Ser Gly Gly Thr Asn Tyr Ala Gln Lys Phe 50 55 60 Gln Gly
Arg Val Thr Met Thr Arg Asp Thr Ser Ile Ser Thr Ala Tyr 65 70 75 80
Met Glu Leu Asn Arg Leu Arg Ser Asp Asp Thr Ala Val Tyr Tyr Cys 85
90 95 Ala Arg Asp Gln Pro Leu Gly Tyr Cys Thr Asn Gly Val Cys Ser
Tyr 100 105 110 Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser
Ser 115 120 125 <210> SEQ ID NO 29 <211> LENGTH: 112
<212> TYPE: PRT <213> ORGANISM: Mus musculus
<400> SEQUENCE: 29 Asp Val Val Val Thr Gln Thr Pro Leu Ser
Leu Pro Val Ser Leu Gly 1 5 10 15 Ala Gln Ala Ser Ile Ser Cys Arg
Ser Ser Gln Ser Leu Val His Ser 20 25 30 Asn Gly Asn Thr Phe Leu
His Trp Tyr Leu Gln Lys Pro Gly Gln Ser 35 40 45 Pro Lys Leu Leu
Ile Tyr Thr Val Ser Asn Arg Phe Ser Gly Val Pro 50 55 60 Asp Arg
Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile 65 70 75 80
Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr Phe Cys Ser Gln Thr 85
90 95 Thr His Val Pro Trp Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile
Gln 100 105 110 <210> SEQ ID NO 30 <211> LENGTH: 114
<212> TYPE: PRT <213> ORGANISM: Mus musculus
<400> SEQUENCE: 30 Glu Val Gln Leu Gln Gln Ser Gly Pro Asp
Leu Val Lys Pro Gly Ala 1 5 10 15 Ser Val Lys Ile Ser Cys Lys Ala
Ser Gly Tyr Ser Phe Thr Gly Tyr 20 25 30 Tyr Ile His Trp Val Lys
Gln Ser His Gly Lys Ser Leu Glu Trp Ile 35 40 45 Gly Arg Val Ile
Pro Asn Asn Gly Gly Thr Ser Tyr Asn Gln Lys Phe 50 55 60 Lys Gly
Lys Ala Ile Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Tyr 65 70 75 80
Met Glu Leu Arg Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys 85
90 95 Ala Arg Glu Gly Ile Tyr Trp Trp Gly His Gly Thr Thr Leu Thr
Val 100 105 110 Ser Ser <210> SEQ ID NO 31 <211>
LENGTH: 112 <212> TYPE: PRT <213> ORGANISM: Mus
musculus <400> SEQUENCE: 31 Asp Ala Val Met Thr Gln Asn Pro
Leu Ser Leu Pro Val Ser Leu Gly 1 5 10 15 Asp Glu Ala Ser Ile Ser
Cys Arg Ser Ser Gln Ser Leu Glu Asn Ser 20 25 30 Asn Gly Asn Thr
Phe Leu Asn Trp Phe Phe Gln Lys Pro Gly Gln Ser 35 40 45 Pro Gln
Leu Leu Ile Tyr Arg Val Ser Asn Arg Phe Ser Gly Val Pro 50 55 60
Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile 65
70 75 80 Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr Phe Cys Leu
Gln Val 85 90 95 Thr His Val Pro Tyr Thr Phe Gly Gly Gly Thr Thr
Leu Glu Ile Lys 100 105 110 <210> SEQ ID NO 32 <211>
LENGTH: 112 <212> TYPE: PRT <213> ORGANISM: Mus
musculus <400> SEQUENCE: 32 Asp Ile Gln Leu Gln Gln Ser Gly
Pro Gly Leu Val Lys Pro Ser Gln 1 5 10 15 Ser Leu Ser Leu Thr Cys
Ser Val Thr Gly Tyr Ser Ile Thr Thr Asn 20 25 30 Tyr Asn Trp Asn
Trp Ile Arg Gln Phe Pro Gly Asn Lys Leu Glu Trp 35 40 45 Met Gly
Tyr Ile Arg Tyr Asp Gly Thr Ser Glu Tyr Thr Pro Ser Leu 50 55 60
Lys Asn Arg Val Ser Ile Thr Arg Asp Thr Ser Met Asn Gln Phe Phe 65
70 75 80 Leu Arg Leu Thr Ser Val Thr Pro Glu Asp Thr Ala Thr Tyr
Tyr Cys 85 90 95 Ala Arg Leu Asp Tyr Trp Gly Gln Gly Thr Ser Val
Thr Val Ser Ser 100 105 110 <210> SEQ ID NO 33 <211>
LENGTH: 112 <212> TYPE: PRT <213> ORGANISM: Mus
musculus <400> SEQUENCE: 33 Glu Leu Gln Leu Thr Gln Ser Pro
Leu Ser Leu Pro Val Ser Leu Gly 1 5 10 15 Asp Gln Ala Ser Ile Ser
Cys Arg Ser Ser Gln Ser Leu Val Asn Ser 20 25 30 Asn Gly Asn Thr
Tyr Leu His Trp Tyr Leu Gln Lys Pro Gly Gln Ser 35 40 45 Pro Lys
Leu Leu Ile Tyr Lys Val Ser Asn Arg Phe Ser Gly Val Pro 50 55 60
Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile 65
70 75 80 Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr Phe Cys Ser
Gln Ser 85 90 95 Thr His Val Pro Trp Thr Phe Gly Gly Gly Thr Lys
Leu Glu Ile Lys 100 105 110 <210> SEQ ID NO 34 <211>
LENGTH: 113 <212> TYPE: PRT <213> ORGANISM: Mus
musculus <400> SEQUENCE: 34 Gln Val Lys Leu Glu Glu Ser Gly
Pro Gly Leu Val Ala Pro Ser Gln 1 5 10 15 Ser Leu Ser Ile Thr Cys
Thr Val Ser Gly Phe Ser Leu Ser Arg Tyr 20 25 30 Ser Val Tyr Trp
Val Arg Gln Pro Pro Gly Lys Gly Leu Glu Trp Leu 35 40 45 Gly Met
Met Trp Gly Gly Gly Ser Thr Asp Tyr Asn Ser Ala Leu Lys 50 55 60
Ser Arg Leu Ser Ile Ser Lys Asp Thr Ser Lys Ser Gln Val Phe Leu 65
70 75 80 Lys Met Asn Ser Leu Gln Thr Asp Asp Thr Ala Met Tyr Tyr
Cys Val 85 90 95 Arg Thr Asp Gly Asp Tyr Trp Gly Gln Gly Thr Ser
Val Thr Val Ser 100 105 110 Ser <210> SEQ ID NO 35
<211> LENGTH: 111 <212> TYPE: PRT <213> ORGANISM:
Mus musculus <400> SEQUENCE: 35 Asp Ile Leu Leu Thr Gln Thr
Pro Ala Ser Leu Ala Val Ser Leu Gly 1 5 10 15 Gln Arg Ala Thr Ile
Ser Cys Lys Ala Ser Gln Ser Val Asp Tyr Asp 20 25 30 Gly Asp Ser
Tyr Leu Asn Trp Tyr Gln Gln Ile Pro Gly Gln Pro Pro 35 40 45 Lys
Leu Leu Ile Tyr Asp Ala Ser Asn Leu Val Ser Gly Ile Pro Pro 50 55
60 Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Asn Ile His
65 70 75 80 Pro Val Glu Lys Val Asp Ala Ala Thr Tyr His Cys Gln Gln
Ser Thr 85 90 95 Glu Asp Pro Trp Thr Phe Gly Gly Gly Thr Lys Leu
Glu Ile Lys 100 105 110 <210> SEQ ID NO 36 <211>
LENGTH: 124 <212> TYPE: PRT <213> ORGANISM: Mus
musculus <400> SEQUENCE: 36 Gln Val Gln Leu Gln Gln Ser Gly
Ala Glu Leu Val Arg Pro Gly Ser 1 5 10 15 Ser Val Lys Ile Ser Cys
Lys Ala Ser Gly Tyr Ala Phe Ser Ser Tyr 20 25 30 Trp Met Asn Trp
Val Lys Gln Arg Pro Gly Gln Gly Leu Glu Trp Ile 35 40 45 Gly Gln
Ile Trp Pro Gly Asp Gly Asp Thr Asn Tyr Asn Gly Lys Phe 50 55 60
Lys Gly Lys Ala Thr Leu Thr Ala Asp Glu Ser Ser Ser Thr Ala Tyr 65
70 75 80 Met Gln Leu Ser Ser Leu Ala Ser Glu Asp Ser Ala Val Tyr
Phe Cys 85 90 95 Ala Arg Arg Glu Thr Thr Thr Val Gly Arg Tyr Tyr
Tyr Ala Met Asp 100 105 110 Tyr Trp Gly Gln Gly Thr Ser Val Thr Val
Ser Ser 115 120 <210> SEQ ID NO 37 <211> LENGTH: 107
<212> TYPE: PRT <213> ORGANISM: Mus musculus
<400> SEQUENCE: 37 Ala Ile Gln Leu Thr Gln Ser Pro Ser Ser
Leu Ser Ala Ser Val Gly 1 5 10 15 Asp Arg Val Thr Ile Thr Cys Arg
Ala Ser Gln Gly Ile Ser Ser Ala 20 25 30 Leu Ala Trp Tyr Gln Gln
Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 35 40 45 Tyr Asp Ala Ser
Ser Leu Glu Ser Gly Val Pro Ser Arg Phe Ser Gly 50 55 60 Ser Gly
Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 65 70 75 80
Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Phe Asn Ser Tyr Pro Tyr 85
90 95 Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys 100 105
<210> SEQ ID NO 38 <211> LENGTH: 120 <212> TYPE:
PRT <213> ORGANISM: Mus musculus <400> SEQUENCE: 38 Glu
Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Glu 1 5 10
15 Ser Leu Lys Ile Ser Cys Lys Gly Ser Gly Tyr Ser Phe Ser Ser Ser
20 25 30 Trp Ile Gly Trp Val Arg Gln Met Pro Gly Lys Gly Leu Glu
Trp Met 35 40 45 Gly Ile Ile Tyr Pro Asp Asp Ser Asp Thr Arg Tyr
Ser Pro Ser Phe 50 55 60 Gln Gly Gln Val Thr Ile Ser Ala Asp Lys
Ser Ile Arg Thr Ala Tyr 65 70 75 80 Leu Gln Trp Ser Ser Leu Lys Ala
Ser Asp Thr Ala Met Tyr Tyr Cys 85 90 95 Ala Arg His Val Thr Met
Trp Gly Val Ile Ile Asp Phe Trp Gly Gln 100 105 110 Gly Thr Leu Val
Thr Val Ser Ser 115 120 <210> SEQ ID NO 39 <211>
LENGTH: 112 <212> TYPE: PRT <213> ORGANISM: Mus
musculus <400> SEQUENCE: 39 Asp Ile Val Met Thr Gln Ala Ala
Pro Ser Ile Pro Val Thr Pro Gly 1 5 10 15 Glu Ser Val Ser Ile Ser
Cys Arg Ser Ser Lys Ser Leu Leu Asn Ser 20 25 30 Asn Gly Asn Thr
Tyr Leu Tyr Trp Phe Leu Gln Arg Pro Gly Gln Ser 35 40 45 Pro Gln
Leu Leu Ile Tyr Arg Met Ser Asn Leu Ala Ser Gly Val Pro 50 55 60
Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Ala Phe Thr Leu Arg Ile 65
70 75 80 Ser Arg Val Glu Ala Glu Asp Val Gly Val Tyr Tyr Cys Met
Gln His 85 90 95 Leu Glu Tyr Pro Phe Thr Phe Gly Ala Gly Thr Lys
Leu Glu Leu Lys 100 105 110 <210> SEQ ID NO 40 <211>
LENGTH: 121 <212> TYPE: PRT <213> ORGANISM: Mus
musculus <400> SEQUENCE: 40 Glu Val Gln Leu Gln Gln Ser Gly
Pro Glu Leu Ile Lys Pro Gly Ala 1 5 10 15 Ser Val Lys Met Ser Cys
Lys Ala Ser Gly Tyr Thr Phe Thr Ser Tyr 20 25 30 Val Met His Trp
Val Lys Gln Lys Pro Gly Gln Gly Leu Glu Trp Ile 35 40 45 Gly Tyr
Ile Asn Pro Tyr Asn Asp Gly Thr Lys Tyr Asn Glu Lys Phe 50 55 60
Lys Gly Lys Ala Thr Leu Thr Ser Asp Lys Ser Ser Ser Thr Ala Tyr 65
70 75 80 Met Glu Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr
Tyr Cys 85 90 95 Ala Arg Gly Thr Tyr Tyr Tyr Gly Ser Arg Val Phe
Asp Tyr Trp Gly 100 105 110 Gln Gly Thr Thr Leu Thr Val Ser Ser 115
120
1 SEQUENCE LISTING <160> NUMBER OF SEQ ID NOS: 40 <210>
SEQ ID NO 1 <211> LENGTH: 112 <212> TYPE: PRT
<213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: Synthesized HuAM4G7 VL <400>
SEQUENCE: 1 Asp Ile Val Met Thr Gln Ser Pro Ala Thr Leu Ser Leu Ser
Pro Gly 1 5 10 15 Glu Arg Ala Thr Leu Ser Cys Arg Ser Ser Lys Ser
Leu Gln Asn Val 20 25 30 Asn Gly Asn Thr Tyr Leu Tyr Trp Phe Gln
Gln Lys Pro Gly Gln Ser 35 40 45 Pro Gln Leu Leu Ile Tyr Arg Met
Ser Asn Leu Asn Ser Gly Val Pro 50 55 60 Asp Arg Phe Ser Gly Ser
Gly Ser Gly Thr Glu Phe Thr Leu Thr Ile 65 70 75 80 Ser Ser Leu Glu
Pro Glu Asp Phe Ala Val Tyr Tyr Cys Met Gln His 85 90 95 Leu Glu
Tyr Pro Ile Thr Phe Gly Ala Gly Thr Lys Leu Glu Ile Lys 100 105 110
<210> SEQ ID NO 2 <211> LENGTH: 121 <212> TYPE:
PRT <213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: Synthesized HuAM4G7 VH <400>
SEQUENCE: 2 Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Lys Pro
Gly Gly 1 5 10 15 Ser Leu Lys Leu Ser Cys Ala Ala Ser Gly Tyr Thr
Phe Thr Ser Tyr 20 25 30 Val Met His Trp Val Arg Gln Ala Pro Gly
Lys Gly Leu Glu Trp Ile 35 40 45 Gly Tyr Ile Asn Pro Tyr Asn Asp
Gly Thr Lys Tyr Asn Glu Lys Phe 50 55 60 Gln Gly Arg Val Thr Ile
Ser Ser Asp Lys Ser Ile Ser Thr Ala Tyr 65 70 75 80 Met Glu Leu Ser
Ser Leu Arg Ser Glu Asp Thr Ala Met Tyr Tyr Cys 85 90 95 Ala Arg
Gly Thr Tyr Tyr Tyr Gly Thr Arg Val Phe Asp Tyr Trp Gly 100 105 110
Gln Gly Thr Leu Val Thr Val Ser Ser 115 120 <210> SEQ ID NO 3
<211> LENGTH: 107 <212> TYPE: PRT <213> ORGANISM:
Homo sapiens <400> SEQUENCE: 3 Arg Thr Val Ala Ala Pro Ser
Val Phe Ile Phe Pro Pro Ser Asp Glu 1 5 10 15 Gln Leu Lys Ser Gly
Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe 20 25 30 Tyr Pro Arg
Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln 35 40 45 Ser
Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser 50 55
60 Thr Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu
65 70 75 80 Lys His Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu
Ser Ser 85 90 95 Pro Val Thr Lys Ser Phe Asn Arg Gly Glu Cys 100
105 <210> SEQ ID NO 4 <211> LENGTH: 330 <212>
TYPE: PRT <213> ORGANISM: Homo sapiens <400> SEQUENCE:
4 Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys 1
5 10 15 Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp
Tyr 20 25 30 Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala
Leu Thr Ser 35 40 45 Gly Val His Thr Phe Pro Ala Val Leu Gln Ser
Ser Gly Leu Tyr Ser 50 55 60 Leu Ser Ser Val Val Thr Val Pro Ser
Ser Ser Leu Gly Thr Gln Thr 65 70 75 80 Tyr Ile Cys Asn Val Asn His
Lys Pro Ser Asn Thr Lys Val Asp Lys 85 90 95 Lys Val Glu Pro Lys
Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys 100 105 110 Pro Ala Pro
Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro 115 120 125 Lys
Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys 130 135
140 Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp
145 150 155 160 Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys
Pro Arg Glu 165 170 175 Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser
Val Leu Thr Val Leu 180 185 190 His Gln Asp Trp Leu Asn Gly Lys Glu
Tyr Lys Cys Lys Val Ser Asn 195 200 205 Lys Ala Leu Pro Ala Pro Ile
Glu Lys Thr Ile Ser Lys Ala Lys Gly 210 215 220 Gln Pro Arg Glu Pro
Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu 225 230 235 240 Met Thr
Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr 245 250 255
Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn 260
265 270 Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe
Phe 275 280 285 Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln
Gln Gly Asn 290 295 300 Val Phe Ser Cys Ser Val Met His Glu Ala Leu
His Asn His Tyr Thr 305 310 315 320 Gln Lys Ser Leu Ser Leu Ser Pro
Gly Lys 325 330 <210> SEQ ID NO 5 <211> LENGTH: 330
<212> TYPE: PRT <213> ORGANISM: Artificial Sequence
<220> FEATURE: <223> OTHER INFORMATION: Synthesized
S267E/L328F IgG1 constant chain <400> SEQUENCE: 5 Ala Ser Thr
Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys 1 5 10 15 Ser
Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 20 25
30 Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser
35 40 45 Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu
Tyr Ser 50 55 60 Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu
Gly Thr Gln Thr 65 70 75 80 Tyr Ile Cys Asn Val Asn His Lys Pro Ser
Asn Thr Lys Val Asp Lys 85 90 95 Lys Val Glu Pro Lys Ser Cys Asp
Lys Thr His Thr Cys Pro Pro Cys 100 105 110 Pro Ala Pro Glu Leu Leu
Gly Gly Pro Ser Val Phe Leu Phe Pro Pro 115 120 125 Lys Pro Lys Asp
Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys 130 135 140 Val Val
Val Asp Val Glu His Glu Asp Pro Glu Val Lys Phe Asn Trp 145 150 155
160 Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu
165 170 175 Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr
Val Leu 180 185 190 His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys
Lys Val Ser Asn 195 200 205 Lys Ala Phe Pro Ala Pro Ile Glu Lys Thr
Ile Ser Lys Ala Lys Gly 210 215 220 Gln Pro Arg Glu Pro Gln Val Tyr
Thr Leu Pro Pro Ser Arg Glu Glu 225 230 235 240 Met Thr Lys Asn Gln
Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr 245 250 255 Pro Ser Asp
Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn 260 265 270 Asn
Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe 275 280
285 Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn
290 295 300 Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His
Tyr Thr 305 310 315 320 Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 325
330 <210> SEQ ID NO 6 <211> LENGTH: 330 <212>
TYPE: PRT <213> ORGANISM: Artificial Sequence <220>
FEATURE: <223> OTHER INFORMATION: Synthesized G236D/S267E
IgG1 constant chain
<400> SEQUENCE: 6 Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu
Ala Pro Ser Ser Lys 1 5 10 15 Ser Thr Ser Gly Gly Thr Ala Ala Leu
Gly Cys Leu Val Lys Asp Tyr 20 25 30 Phe Pro Glu Pro Val Thr Val
Ser Trp Asn Ser Gly Ala Leu Thr Ser 35 40 45 Gly Val His Thr Phe
Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 50 55 60 Leu Ser Ser
Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr 65 70 75 80 Tyr
Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys 85 90
95 Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys
100 105 110 Pro Ala Pro Glu Leu Leu Asp Gly Pro Ser Val Phe Leu Phe
Pro Pro 115 120 125 Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro
Glu Val Thr Cys 130 135 140 Val Val Val Asp Val Glu His Glu Asp Pro
Glu Val Lys Phe Asn Trp 145 150 155 160 Tyr Val Asp Gly Val Glu Val
His Asn Ala Lys Thr Lys Pro Arg Glu 165 170 175 Glu Gln Tyr Asn Ser
Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu 180 185 190 His Gln Asp
Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn 195 200 205 Lys
Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly 210 215
220 Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu
225 230 235 240 Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys
Gly Phe Tyr 245 250 255 Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn
Gly Gln Pro Glu Asn 260 265 270 Asn Tyr Lys Thr Thr Pro Pro Val Leu
Asp Ser Asp Gly Ser Phe Phe 275 280 285 Leu Tyr Ser Lys Leu Thr Val
Asp Lys Ser Arg Trp Gln Gln Gly Asn 290 295 300 Val Phe Ser Cys Ser
Val Met His Glu Ala Leu His Asn His Tyr Thr 305 310 315 320 Gln Lys
Ser Leu Ser Leu Ser Pro Gly Lys 325 330 <210> SEQ ID NO 7
<211> LENGTH: 219 <212> TYPE: PRT <213> ORGANISM:
Artificial Sequence <220> FEATURE: <223> OTHER
INFORMATION: Synthesized HuAM4G7 light chain (VH-C ) <400>
SEQUENCE: 7 Asp Ile Val Met Thr Gln Ser Pro Ala Thr Leu Ser Leu Ser
Pro Gly 1 5 10 15 Glu Arg Ala Thr Leu Ser Cys Arg Ser Ser Lys Ser
Leu Gln Asn Val 20 25 30 Asn Gly Asn Thr Tyr Leu Tyr Trp Phe Gln
Gln Lys Pro Gly Gln Ser 35 40 45 Pro Gln Leu Leu Ile Tyr Arg Met
Ser Asn Leu Asn Ser Gly Val Pro 50 55 60 Asp Arg Phe Ser Gly Ser
Gly Ser Gly Thr Glu Phe Thr Leu Thr Ile 65 70 75 80 Ser Ser Leu Glu
Pro Glu Asp Phe Ala Val Tyr Tyr Cys Met Gln His 85 90 95 Leu Glu
Tyr Pro Ile Thr Phe Gly Ala Gly Thr Lys Leu Glu Ile Lys 100 105 110
Arg Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu 115
120 125 Gln Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn
Phe 130 135 140 Tyr Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn
Ala Leu Gln 145 150 155 160 Ser Gly Asn Ser Gln Glu Ser Val Thr Glu
Gln Asp Ser Lys Asp Ser 165 170 175 Thr Tyr Ser Leu Ser Ser Thr Leu
Thr Leu Ser Lys Ala Asp Tyr Glu 180 185 190 Lys His Lys Val Tyr Ala
Cys Glu Val Thr His Gln Gly Leu Ser Ser 195 200 205 Pro Val Thr Lys
Ser Phe Asn Arg Gly Glu Cys 210 215 <210> SEQ ID NO 8
<211> LENGTH: 451 <212> TYPE: PRT <213> ORGANISM:
Artificial Sequence <220> FEATURE: <223> OTHER
INFORMATION: Synthesized HuAM4G7 IgG1 heavy chain <400>
SEQUENCE: 8 Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Lys Pro
Gly Gly 1 5 10 15 Ser Leu Lys Leu Ser Cys Ala Ala Ser Gly Tyr Thr
Phe Thr Ser Tyr 20 25 30 Val Met His Trp Val Arg Gln Ala Pro Gly
Lys Gly Leu Glu Trp Ile 35 40 45 Gly Tyr Ile Asn Pro Tyr Asn Asp
Gly Thr Lys Tyr Asn Glu Lys Phe 50 55 60 Gln Gly Arg Val Thr Ile
Ser Ser Asp Lys Ser Ile Ser Thr Ala Tyr 65 70 75 80 Met Glu Leu Ser
Ser Leu Arg Ser Glu Asp Thr Ala Met Tyr Tyr Cys 85 90 95 Ala Arg
Gly Thr Tyr Tyr Tyr Gly Thr Arg Val Phe Asp Tyr Trp Gly 100 105 110
Gln Gly Thr Leu Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser 115
120 125 Val Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr
Ala 130 135 140 Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro
Val Thr Val 145 150 155 160 Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly
Val His Thr Phe Pro Ala 165 170 175 Val Leu Gln Ser Ser Gly Leu Tyr
Ser Leu Ser Ser Val Val Thr Val 180 185 190 Pro Ser Ser Ser Leu Gly
Thr Gln Thr Tyr Ile Cys Asn Val Asn His 195 200 205 Lys Pro Ser Asn
Thr Lys Val Asp Lys Lys Val Glu Pro Lys Ser Cys 210 215 220 Asp Lys
Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly 225 230 235
240 Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met
245 250 255 Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val
Ser His 260 265 270 Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp
Gly Val Glu Val 275 280 285 His Asn Ala Lys Thr Lys Pro Arg Glu Glu
Gln Tyr Asn Ser Thr Tyr 290 295 300 Arg Val Val Ser Val Leu Thr Val
Leu His Gln Asp Trp Leu Asn Gly 305 310 315 320 Lys Glu Tyr Lys Cys
Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile 325 330 335 Glu Lys Thr
Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val 340 345 350 Tyr
Thr Leu Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser 355 360
365 Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu
370 375 380 Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr
Pro Pro 385 390 395 400 Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr
Ser Lys Leu Thr Val 405 410 415 Asp Lys Ser Arg Trp Gln Gln Gly Asn
Val Phe Ser Cys Ser Val Met 420 425 430 His Glu Ala Leu His Asn His
Tyr Thr Gln Lys Ser Leu Ser Leu Ser 435 440 445 Pro Gly Lys 450
<210> SEQ ID NO 9 <211> LENGTH: 451 <212> TYPE:
PRT <213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: Synthesized HuAM4G7 S267E/L328F
heavy chain <400> SEQUENCE: 9 Glu Val Gln Leu Val Glu Ser Gly
Gly Gly Leu Val Lys Pro Gly Gly 1 5 10 15 Ser Leu Lys Leu Ser Cys
Ala Ala Ser Gly Tyr Thr Phe Thr Ser Tyr 20 25 30 Val Met His Trp
Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Ile 35 40 45 Gly Tyr
Ile Asn Pro Tyr Asn Asp Gly Thr Lys Tyr Asn Glu Lys Phe 50 55 60
Gln Gly Arg Val Thr Ile Ser Ser Asp Lys Ser Ile Ser Thr Ala Tyr 65
70 75 80 Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Met Tyr
Tyr Cys 85 90 95 Ala Arg Gly Thr Tyr Tyr Tyr Gly Thr Arg Val Phe
Asp Tyr Trp Gly 100 105 110 Gln Gly Thr Leu Val Thr Val Ser Ser Ala
Ser Thr Lys Gly Pro Ser 115 120 125 Val Phe Pro Leu Ala Pro Ser Ser
Lys Ser Thr Ser Gly Gly Thr Ala
130 135 140 Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val
Thr Val 145 150 155 160 Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val
His Thr Phe Pro Ala 165 170 175 Val Leu Gln Ser Ser Gly Leu Tyr Ser
Leu Ser Ser Val Val Thr Val 180 185 190 Pro Ser Ser Ser Leu Gly Thr
Gln Thr Tyr Ile Cys Asn Val Asn His 195 200 205 Lys Pro Ser Asn Thr
Lys Val Asp Lys Lys Val Glu Pro Lys Ser Cys 210 215 220 Asp Lys Thr
His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly 225 230 235 240
Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met 245
250 255 Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Glu
His 260 265 270 Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly
Val Glu Val 275 280 285 His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln
Tyr Asn Ser Thr Tyr 290 295 300 Arg Val Val Ser Val Leu Thr Val Leu
His Gln Asp Trp Leu Asn Gly 305 310 315 320 Lys Glu Tyr Lys Cys Lys
Val Ser Asn Lys Ala Phe Pro Ala Pro Ile 325 330 335 Glu Lys Thr Ile
Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val 340 345 350 Tyr Thr
Leu Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser 355 360 365
Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu 370
375 380 Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro
Pro 385 390 395 400 Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser
Lys Leu Thr Val 405 410 415 Asp Lys Ser Arg Trp Gln Gln Gly Asn Val
Phe Ser Cys Ser Val Met 420 425 430 His Glu Ala Leu His Asn His Tyr
Thr Gln Lys Ser Leu Ser Leu Ser 435 440 445 Pro Gly Lys 450
<210> SEQ ID NO 10 <211> LENGTH: 451 <212> TYPE:
PRT <213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: Synthesized HuAM4G7 G236D/S267E
heavy chain <400> SEQUENCE: 10 Glu Val Gln Leu Val Glu Ser
Gly Gly Gly Leu Val Lys Pro Gly Gly 1 5 10 15 Ser Leu Lys Leu Ser
Cys Ala Ala Ser Gly Tyr Thr Phe Thr Ser Tyr 20 25 30 Val Met His
Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Ile 35 40 45 Gly
Tyr Ile Asn Pro Tyr Asn Asp Gly Thr Lys Tyr Asn Glu Lys Phe 50 55
60 Gln Gly Arg Val Thr Ile Ser Ser Asp Lys Ser Ile Ser Thr Ala Tyr
65 70 75 80 Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Met Tyr
Tyr Cys 85 90 95 Ala Arg Gly Thr Tyr Tyr Tyr Gly Thr Arg Val Phe
Asp Tyr Trp Gly 100 105 110 Gln Gly Thr Leu Val Thr Val Ser Ser Ala
Ser Thr Lys Gly Pro Ser 115 120 125 Val Phe Pro Leu Ala Pro Ser Ser
Lys Ser Thr Ser Gly Gly Thr Ala 130 135 140 Ala Leu Gly Cys Leu Val
Lys Asp Tyr Phe Pro Glu Pro Val Thr Val 145 150 155 160 Ser Trp Asn
Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala 165 170 175 Val
Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val 180 185
190 Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His
195 200 205 Lys Pro Ser Asn Thr Lys Val Asp Lys Lys Val Glu Pro Lys
Ser Cys 210 215 220 Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro
Glu Leu Leu Asp 225 230 235 240 Gly Pro Ser Val Phe Leu Phe Pro Pro
Lys Pro Lys Asp Thr Leu Met 245 250 255 Ile Ser Arg Thr Pro Glu Val
Thr Cys Val Val Val Asp Val Glu His 260 265 270 Glu Asp Pro Glu Val
Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val 275 280 285 His Asn Ala
Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr 290 295 300 Arg
Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly 305 310
315 320 Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro
Ile 325 330 335 Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu
Pro Gln Val 340 345 350 Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Thr
Lys Asn Gln Val Ser 355 360 365 Leu Thr Cys Leu Val Lys Gly Phe Tyr
Pro Ser Asp Ile Ala Val Glu 370 375 380 Trp Glu Ser Asn Gly Gln Pro
Glu Asn Asn Tyr Lys Thr Thr Pro Pro 385 390 395 400 Val Leu Asp Ser
Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val 405 410 415 Asp Lys
Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met 420 425 430
His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser 435
440 445 Pro Gly Lys 450 <210> SEQ ID NO 11 <211>
LENGTH: 106 <212> TYPE: PRT <213> ORGANISM: Artificial
Sequence <220> FEATURE: <223> OTHER INFORMATION:
Synthesized anti-RSV Numax VL <400> SEQUENCE: 11 Asp Ile Gln
Met Thr Gln Ser Pro Ser Thr Leu Ser Ala Ser Val Gly 1 5 10 15 Asp
Arg Val Thr Ile Thr Cys Ser Ala Ser Ser Arg Val Gly Tyr Met 20 25
30 His Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile Tyr
35 40 45 Asp Thr Ser Lys Leu Ala Ser Gly Val Pro Ser Arg Phe Ser
Gly Ser 50 55 60 Gly Ser Gly Thr Glu Phe Thr Leu Thr Ile Ser Ser
Leu Gln Pro Asp 65 70 75 80 Asp Phe Ala Thr Tyr Tyr Cys Phe Gln Gly
Ser Gly Tyr Pro Phe Thr 85 90 95 Phe Gly Gly Gly Thr Lys Val Glu
Ile Lys 100 105 <210> SEQ ID NO 12 <211> LENGTH: 120
<212> TYPE: PRT <213> ORGANISM: Artificial Sequence
<220> FEATURE: <223> OTHER INFORMATION: Synthesized
anti-RSV Numax VH <400> SEQUENCE: 12 Gln Val Thr Leu Arg Glu
Ser Gly Pro Ala Leu Val Lys Pro Thr Gln 1 5 10 15 Thr Leu Thr Leu
Thr Cys Thr Phe Ser Gly Phe Ser Leu Ser Thr Ala 20 25 30 Gly Met
Ser Val Gly Trp Ile Arg Gln Pro Pro Gly Lys Ala Leu Glu 35 40 45
Trp Leu Ala Asp Ile Trp Trp Asp Asp Lys Lys His Tyr Asn Pro Ser 50
55 60 Leu Lys Asp Arg Leu Thr Ile Ser Lys Asp Thr Ser Lys Asn Gln
Val 65 70 75 80 Val Leu Lys Val Thr Asn Met Asp Pro Ala Asp Thr Ala
Thr Tyr Tyr 85 90 95 Cys Ala Arg Asp Met Ile Phe Asn Phe Tyr Phe
Asp Val Trp Gly Gln 100 105 110 Gly Thr Thr Val Thr Val Ser Ser 115
120 <210> SEQ ID NO 13 <211> LENGTH: 112 <212>
TYPE: PRT <213> ORGANISM: Artificial Sequence <220>
FEATURE: <223> OTHER INFORMATION: Synthesized anti-FITC VL
<400> SEQUENCE: 13 Asp Val Val Met Thr Gln Thr Pro Leu Ser
Leu Pro Val Ser Leu Gly 1 5 10 15 Asp Gln Ala Ser Ile Ser Cys Arg
Ser Ser Gln Ser Leu Val His Ser 20 25 30 Asn Gly Asn Thr Tyr Leu
Arg Trp Tyr Leu Gln Lys Pro Gly Gln Ser 35 40 45 Pro Lys Val Leu
Ile Tyr Lys Val Ser Asn Arg Phe Ser Gly Val Pro 50 55 60 Asp Arg
Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile 65 70 75 80
Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr Phe Cys Ser Gln Ser
85 90 95 Thr His Val Pro Trp Thr Phe Gly Gly Gly Thr Lys Leu Glu
Ile Lys 100 105 110 <210> SEQ ID NO 14 <211> LENGTH:
118 <212> TYPE: PRT <213> ORGANISM: Artificial Sequence
<220> FEATURE: <223> OTHER INFORMATION: Synthesized
anti-FITC VH <400> SEQUENCE: 14 Glu Val Lys Leu Asp Glu Thr
Gly Gly Gly Leu Val Gln Pro Gly Arg 1 5 10 15 Pro Met Lys Leu Ser
Cys Val Ala Ser Gly Phe Thr Phe Ser Asp Tyr 20 25 30 Trp Met Asn
Trp Val Arg Gln Ser Pro Glu Lys Gly Leu Glu Trp Val 35 40 45 Ala
Gln Ile Arg Asn Lys Pro Tyr Asn Tyr Glu Thr Tyr Tyr Ser Asp 50 55
60 Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asp Ser Lys Ser Ser
65 70 75 80 Val Tyr Leu Gln Met Asn Asn Leu Arg Val Glu Asp Met Gly
Ile Tyr 85 90 95 Tyr Cys Thr Gly Ser Tyr Tyr Gly Met Asp Tyr Trp
Gly Gln Gly Thr 100 105 110 Ser Val Thr Val Ser Ser 115 <210>
SEQ ID NO 15 <211> LENGTH: 111 <212> TYPE: PRT
<213> ORGANISM: Mus musculus <400> SEQUENCE: 15 Asp Ile
Val Leu Thr Gln Ser Pro Ala Ser Leu Ala Val Ser Leu Gly 1 5 10 15
Gln Arg Ala Thr Ile Ser Cys Lys Ala Ser Gln Ser Val Asp Tyr Asp 20
25 30 Gly Asp Ser Phe Leu Asn Trp Tyr Gln Gln Lys Pro Gly Gln Pro
Pro 35 40 45 Lys Leu Phe Ile Tyr Ala Ala Ser Asn Leu Glu Ser Gly
Ile Pro Ala 50 55 60 Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe
Thr Leu Asn Ile His 65 70 75 80 Pro Val Glu Glu Glu Asp Ala Ala Thr
Tyr Tyr Cys Gln Gln Ser Asn 85 90 95 Glu Asp Pro Leu Thr Phe Gly
Ala Gly Thr Glu Leu Glu Leu Lys 100 105 110 <210> SEQ ID NO
16 <211> LENGTH: 117 <212> TYPE: PRT <213>
ORGANISM: Mus musculus <400> SEQUENCE: 16 Glu Val Gln Leu Gln
Gln Ser Gly Ala Glu Leu Met Lys Pro Gly Ala 1 5 10 15 Ser Val Lys
Ile Ser Cys Lys Ala Thr Gly Tyr Thr Phe Ser Ser Tyr 20 25 30 Trp
Ile Glu Trp Val Lys Gln Arg Pro Gly His Gly Leu Glu Trp Ile 35 40
45 Gly Glu Ile Leu Pro Gly Gly Gly Asp Thr Asn Tyr Asn Glu Ile Phe
50 55 60 Lys Gly Lys Ala Thr Phe Thr Ala Asp Thr Ser Ser Asn Thr
Ala Tyr 65 70 75 80 Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala
Val Tyr Tyr Cys 85 90 95 Thr Arg Arg Val Pro Val Tyr Phe Asp Tyr
Trp Gly Gln Gly Thr Ser 100 105 110 Val Thr Val Ser Ser 115
<210> SEQ ID NO 17 <211> LENGTH: 106 <212> TYPE:
PRT <213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: Synthesized anti-CD20 Pro70769 VL
<400> SEQUENCE: 17 Asp Ile Gln Met Thr Gln Ser Pro Ser Ser
Leu Ser Ala Ser Val Gly 1 5 10 15 Asp Arg Val Thr Ile Thr Cys Arg
Ala Ser Ser Ser Val Ser Tyr Met 20 25 30 His Trp Tyr Gln Gln Lys
Pro Gly Lys Ala Pro Lys Pro Leu Ile Tyr 35 40 45 Ala Pro Ser Asn
Leu Ala Ser Gly Val Pro Ser Arg Phe Ser Gly Ser 50 55 60 Gly Ser
Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro Glu 65 70 75 80
Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Trp Ser Phe Asn Pro Pro Thr 85
90 95 Phe Gly Gln Gly Thr Lys Val Glu Ile Lys 100 105 <210>
SEQ ID NO 18 <211> LENGTH: 122 <212> TYPE: PRT
<213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: Synthesized anti-CD20 Pro70769 VH
<400> SEQUENCE: 18 Glu Val Gln Leu Val Glu Ser Gly Gly Gly
Leu Val Gln Pro Gly Gly 1 5 10 15 Ser Leu Arg Leu Ser Cys Ala Ala
Ser Gly Tyr Thr Phe Thr Ser Tyr 20 25 30 Asn Met His Trp Val Arg
Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 35 40 45 Gly Ala Ile Tyr
Pro Gly Asn Gly Asp Thr Ser Tyr Asn Gln Lys Phe 50 55 60 Lys Gly
Arg Phe Thr Ile Ser Val Asp Lys Ser Lys Asn Thr Leu Tyr 65 70 75 80
Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 85
90 95 Ala Arg Val Val Tyr Tyr Ser Asn Ser Tyr Trp Tyr Phe Asp Val
Trp 100 105 110 Gly Gln Gly Thr Leu Val Thr Val Ser Ser 115 120
<210> SEQ ID NO 19 <211> LENGTH: 107 <212> TYPE:
PRT <213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: Synthesized anti-CD52 Campath VL
<400> SEQUENCE: 19 Asp Ile Gln Met Thr Gln Ser Pro Ser Ser
Leu Ser Ala Ser Val Gly 1 5 10 15 Asp Arg Val Thr Ile Thr Cys Lys
Ala Ser Gln Asn Ile Asp Lys Tyr 20 25 30 Leu Asn Trp Tyr Gln Gln
Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 35 40 45 Tyr Asn Thr Asn
Asn Leu Gln Thr Gly Val Pro Ser Arg Phe Ser Gly 50 55 60 Ser Gly
Ser Gly Thr Asp Phe Thr Phe Thr Ile Ser Ser Leu Gln Pro 65 70 75 80
Glu Asp Ile Ala Thr Tyr Tyr Cys Leu Gln His Ile Ser Arg Pro Arg 85
90 95 Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys 100 105
<210> SEQ ID NO 20 <211> LENGTH: 121 <212> TYPE:
PRT <213> ORGANISM: Artificial Sequence <220> FEATURE:
<223> OTHER INFORMATION: Synthesized anti-CD52 Campath VH
<400> SEQUENCE: 20 Gln Val Gln Leu Gln Glu Ser Gly Pro Gly
Leu Val Arg Pro Ser Gln 1 5 10 15 Thr Leu Ser Leu Thr Cys Thr Val
Ser Gly Phe Thr Phe Thr Asp Phe 20 25 30 Tyr Met Asn Trp Val Arg
Gln Pro Pro Gly Arg Gly Leu Glu Trp Ile 35 40 45 Gly Phe Ile Arg
Asp Lys Ala Lys Gly Tyr Thr Thr Glu Tyr Asn Pro 50 55 60 Ser Val
Lys Gly Arg Val Thr Met Leu Val Asp Thr Ser Lys Asn Gln 65 70 75 80
Phe Ser Leu Arg Leu Ser Ser Val Thr Ala Ala Asp Thr Ala Val Tyr 85
90 95 Tyr Cys Ala Arg Glu Gly His Thr Ala Ala Pro Phe Asp Tyr Trp
Gly 100 105 110 Gln Gly Ser Leu Val Thr Val Ser Ser 115 120
<210> SEQ ID NO 21 <211> LENGTH: 107 <212> TYPE:
PRT <213> ORGANISM: Mus musculus <400> SEQUENCE: 21 Asp
Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 1 5 10
15 Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Asp Ile Arg Tyr Tyr
20 25 30 Leu Asn Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu
Leu Ile 35 40 45 Tyr Val Ala Ser Ser Leu Gln Ser Gly Val Pro Ser
Arg Phe Ser Gly 50 55 60 Ser Gly Ser Gly Thr Glu Phe Thr Leu Thr
Val Ser Ser Leu Gln Pro 65 70 75 80 Glu Asp Phe Ala Thr Tyr Tyr Cys
Leu Gln Val Tyr Ser Thr Pro Arg 85 90 95 Thr Phe Gly Gln Gly Thr
Lys Val Glu Ile Lys
100 105 <210> SEQ ID NO 22 <211> LENGTH: 118
<212> TYPE: PRT <213> ORGANISM: Mus musculus
<400> SEQUENCE: 22 Glu Val Gln Leu Val Glu Ser Gly Gly Gly
Leu Ala Lys Pro Gly Gly 1 5 10 15 Ser Leu Arg Leu Ser Cys Ala Ala
Ser Gly Phe Arg Phe Thr Phe Asn 20 25 30 Asn Tyr Tyr Met Asp Trp
Val Arg Gln Ala Pro Gly Gln Gly Leu Glu 35 40 45 Trp Val Ser Arg
Ile Ser Ser Ser Gly Asp Pro Thr Trp Tyr Ala Asp 50 55 60 Ser Val
Lys Gly Arg Phe Thr Ile Ser Arg Glu Asn Ala Asn Asn Thr 65 70 75 80
Leu Phe Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr 85
90 95 Tyr Cys Ala Ser Leu Thr Thr Gly Ser Asp Ser Trp Gly Gln Gly
Val 100 105 110 Leu Val Thr Val Ser Ser 115 <210> SEQ ID NO
23 <211> LENGTH: 112 <212> TYPE: PRT <213>
ORGANISM: Mus musculus <400> SEQUENCE: 23 Asp Ile Val Ile Thr
Gln Asp Glu Leu Ser Asn Pro Val Thr Ser Gly 1 5 10 15 Glu Ser Val
Ser Ile Ser Cys Arg Ser Ser Lys Ser Leu Leu Tyr Lys 20 25 30 Asp
Gly Lys Thr Tyr Leu Asn Trp Phe Leu Gln Arg Pro Gly Gln Ser 35 40
45 Pro Gln Leu Leu Met Tyr Leu Met Ser Thr Arg Ala Ser Gly Val Ser
50 55 60 Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu
Glu Ile 65 70 75 80 Ser Arg Val Lys Ala Glu Asp Val Gly Val Tyr Tyr
Cys Gln Gln Leu 85 90 95 Val Glu Tyr Pro Phe Thr Phe Gly Ser Gly
Thr Lys Leu Glu Ile Lys 100 105 110 <210> SEQ ID NO 24
<211> LENGTH: 114 <212> TYPE: PRT <213> ORGANISM:
Mus musculus <400> SEQUENCE: 24 Glu Val Lys Leu Glu Glu Ser
Gly Gly Gly Leu Val Gln Pro Gly Gly 1 5 10 15 Ser Met Lys Leu Ser
Cys Val Ala Ser Gly Phe Thr Phe Ser Gly Tyr 20 25 30 Trp Met Ser
Trp Val Arg Gln Ser Pro Glu Lys Gly Leu Glu Trp Val 35 40 45 Ala
Glu Ile Arg Leu Lys Ser Asp Asn Tyr Ala Thr His Tyr Ala Glu 50 55
60 Ser Val Lys Gly Lys Phe Thr Ile Ser Arg Asp Asp Ser Lys Ser Arg
65 70 75 80 Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Ser Gly
Val Tyr 85 90 95 Tyr Cys Thr Asp Phe Ile Asp Trp Gly Gln Gly Thr
Leu Val Thr Val 100 105 110 Ser Ser <210> SEQ ID NO 25
<211> LENGTH: 107 <212> TYPE: PRT <213> ORGANISM:
Mus musculus <400> SEQUENCE: 25 Asp Ile Gln Met Thr Gln Thr
Thr Ser Ser Leu Ser Ala Ser Leu Gly 1 5 10 15 Asp Arg Val Thr Ile
Ser Cys Arg Ala Ser Gln Asp Ile Ser Asn Tyr 20 25 30 Leu Asn Trp
Tyr Gln Gln Lys Pro Asp Gly Thr Val Lys Leu Leu Ile 35 40 45 Tyr
Tyr Thr Ser Ile Leu His Ser Gly Val Pro Ser Arg Phe Ser Gly 50 55
60 Ser Gly Ser Gly Thr Asp Tyr Ser Leu Thr Ile Ser Asn Leu Glu Gln
65 70 75 80 Glu Asp Phe Ala Thr Tyr Phe Cys Gln Gln Gly Asn Thr Leu
Pro Trp 85 90 95 Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys 100
105 <210> SEQ ID NO 26 <211> LENGTH: 123 <212>
TYPE: PRT <213> ORGANISM: Mus musculus <400> SEQUENCE:
26 Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Lys Pro Gly Gly
1 5 10 15 Ser Leu Lys Leu Ser Cys Ala Ala Ser Gly Phe Ala Phe Ser
Ile Tyr 20 25 30 Asp Met Ser Trp Val Arg Gln Thr Pro Glu Lys Arg
Leu Glu Trp Val 35 40 45 Ala Tyr Ile Ser Ser Gly Gly Gly Thr Thr
Tyr Tyr Pro Asp Thr Val 50 55 60 Lys Gly Arg Phe Thr Ile Ser Arg
Asp Asn Ala Lys Asn Thr Leu Tyr 65 70 75 80 Leu Gln Met Ser Ser Leu
Lys Ser Glu Asp Thr Ala Met Tyr Tyr Cys 85 90 95 Ala Arg His Ser
Gly Tyr Gly Ser Ser Tyr Gly Val Leu Phe Ala Tyr 100 105 110 Trp Gly
Gln Gly Thr Leu Val Thr Thr Ser Ala 115 120 <210> SEQ ID NO
27 <211> LENGTH: 107 <212> TYPE: PRT <213>
ORGANISM: Homo sapiens <400> SEQUENCE: 27 Asp Ile Gln Met Thr
Gln Ser Pro Ser Ser Val Ser Ala Ser Val Gly 1 5 10 15 Asp Arg Val
Thr Ile Thr Cys Arg Ala Ser Gln Gly Ile Tyr Ser Trp 20 25 30 Leu
Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Asn Leu Leu Ile 35 40
45 Tyr Thr Ala Ser Thr Leu Gln Ser Gly Val Pro Ser Arg Phe Ser Gly
50 55 60 Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu
Gln Pro 65 70 75 80 Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Ala Asn
Ile Phe Pro Leu 85 90 95 Thr Phe Gly Gly Gly Thr Lys Val Glu Ile
Lys 100 105 <210> SEQ ID NO 28 <211> LENGTH: 126
<212> TYPE: PRT <213> ORGANISM: Homo sapiens
<400> SEQUENCE: 28 Gln Val Gln Leu Val Gln Ser Gly Ala Glu
Val Lys Lys Pro Gly Ala 1 5 10 15 Ser Val Lys Val Ser Cys Lys Ala
Ser Gly Tyr Thr Phe Thr Gly Tyr 20 25 30 Tyr Met His Trp Val Arg
Gln Ala Pro Gly Gln Gly Leu Glu Trp Met 35 40 45 Gly Trp Ile Asn
Pro Asp Ser Gly Gly Thr Asn Tyr Ala Gln Lys Phe 50 55 60 Gln Gly
Arg Val Thr Met Thr Arg Asp Thr Ser Ile Ser Thr Ala Tyr 65 70 75 80
Met Glu Leu Asn Arg Leu Arg Ser Asp Asp Thr Ala Val Tyr Tyr Cys 85
90 95 Ala Arg Asp Gln Pro Leu Gly Tyr Cys Thr Asn Gly Val Cys Ser
Tyr 100 105 110 Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser
Ser 115 120 125 <210> SEQ ID NO 29 <211> LENGTH: 112
<212> TYPE: PRT <213> ORGANISM: Mus musculus
<400> SEQUENCE: 29 Asp Val Val Val Thr Gln Thr Pro Leu Ser
Leu Pro Val Ser Leu Gly 1 5 10 15 Ala Gln Ala Ser Ile Ser Cys Arg
Ser Ser Gln Ser Leu Val His Ser 20 25 30 Asn Gly Asn Thr Phe Leu
His Trp Tyr Leu Gln Lys Pro Gly Gln Ser 35 40 45 Pro Lys Leu Leu
Ile Tyr Thr Val Ser Asn Arg Phe Ser Gly Val Pro 50 55 60 Asp Arg
Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile 65 70 75 80
Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr Phe Cys Ser Gln Thr 85
90 95 Thr His Val Pro Trp Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile
Gln 100 105 110 <210> SEQ ID NO 30 <211> LENGTH: 114
<212> TYPE: PRT <213> ORGANISM: Mus musculus
<400> SEQUENCE: 30
Glu Val Gln Leu Gln Gln Ser Gly Pro Asp Leu Val Lys Pro Gly Ala 1 5
10 15 Ser Val Lys Ile Ser Cys Lys Ala Ser Gly Tyr Ser Phe Thr Gly
Tyr 20 25 30 Tyr Ile His Trp Val Lys Gln Ser His Gly Lys Ser Leu
Glu Trp Ile 35 40 45 Gly Arg Val Ile Pro Asn Asn Gly Gly Thr Ser
Tyr Asn Gln Lys Phe 50 55 60 Lys Gly Lys Ala Ile Leu Thr Val Asp
Lys Ser Ser Ser Thr Ala Tyr 65 70 75 80 Met Glu Leu Arg Ser Leu Thr
Ser Glu Asp Ser Ala Val Tyr Tyr Cys 85 90 95 Ala Arg Glu Gly Ile
Tyr Trp Trp Gly His Gly Thr Thr Leu Thr Val 100 105 110 Ser Ser
<210> SEQ ID NO 31 <211> LENGTH: 112 <212> TYPE:
PRT <213> ORGANISM: Mus musculus <400> SEQUENCE: 31 Asp
Ala Val Met Thr Gln Asn Pro Leu Ser Leu Pro Val Ser Leu Gly 1 5 10
15 Asp Glu Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Leu Glu Asn Ser
20 25 30 Asn Gly Asn Thr Phe Leu Asn Trp Phe Phe Gln Lys Pro Gly
Gln Ser 35 40 45 Pro Gln Leu Leu Ile Tyr Arg Val Ser Asn Arg Phe
Ser Gly Val Pro 50 55 60 Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr
Asp Phe Thr Leu Lys Ile 65 70 75 80 Ser Arg Val Glu Ala Glu Asp Leu
Gly Val Tyr Phe Cys Leu Gln Val 85 90 95 Thr His Val Pro Tyr Thr
Phe Gly Gly Gly Thr Thr Leu Glu Ile Lys 100 105 110 <210> SEQ
ID NO 32 <211> LENGTH: 112 <212> TYPE: PRT <213>
ORGANISM: Mus musculus <400> SEQUENCE: 32 Asp Ile Gln Leu Gln
Gln Ser Gly Pro Gly Leu Val Lys Pro Ser Gln 1 5 10 15 Ser Leu Ser
Leu Thr Cys Ser Val Thr Gly Tyr Ser Ile Thr Thr Asn 20 25 30 Tyr
Asn Trp Asn Trp Ile Arg Gln Phe Pro Gly Asn Lys Leu Glu Trp 35 40
45 Met Gly Tyr Ile Arg Tyr Asp Gly Thr Ser Glu Tyr Thr Pro Ser Leu
50 55 60 Lys Asn Arg Val Ser Ile Thr Arg Asp Thr Ser Met Asn Gln
Phe Phe 65 70 75 80 Leu Arg Leu Thr Ser Val Thr Pro Glu Asp Thr Ala
Thr Tyr Tyr Cys 85 90 95 Ala Arg Leu Asp Tyr Trp Gly Gln Gly Thr
Ser Val Thr Val Ser Ser 100 105 110 <210> SEQ ID NO 33
<211> LENGTH: 112 <212> TYPE: PRT <213> ORGANISM:
Mus musculus <400> SEQUENCE: 33 Glu Leu Gln Leu Thr Gln Ser
Pro Leu Ser Leu Pro Val Ser Leu Gly 1 5 10 15 Asp Gln Ala Ser Ile
Ser Cys Arg Ser Ser Gln Ser Leu Val Asn Ser 20 25 30 Asn Gly Asn
Thr Tyr Leu His Trp Tyr Leu Gln Lys Pro Gly Gln Ser 35 40 45 Pro
Lys Leu Leu Ile Tyr Lys Val Ser Asn Arg Phe Ser Gly Val Pro 50 55
60 Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile
65 70 75 80 Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr Phe Cys Ser
Gln Ser 85 90 95 Thr His Val Pro Trp Thr Phe Gly Gly Gly Thr Lys
Leu Glu Ile Lys 100 105 110 <210> SEQ ID NO 34 <211>
LENGTH: 113 <212> TYPE: PRT <213> ORGANISM: Mus
musculus <400> SEQUENCE: 34 Gln Val Lys Leu Glu Glu Ser Gly
Pro Gly Leu Val Ala Pro Ser Gln 1 5 10 15 Ser Leu Ser Ile Thr Cys
Thr Val Ser Gly Phe Ser Leu Ser Arg Tyr 20 25 30 Ser Val Tyr Trp
Val Arg Gln Pro Pro Gly Lys Gly Leu Glu Trp Leu 35 40 45 Gly Met
Met Trp Gly Gly Gly Ser Thr Asp Tyr Asn Ser Ala Leu Lys 50 55 60
Ser Arg Leu Ser Ile Ser Lys Asp Thr Ser Lys Ser Gln Val Phe Leu 65
70 75 80 Lys Met Asn Ser Leu Gln Thr Asp Asp Thr Ala Met Tyr Tyr
Cys Val 85 90 95 Arg Thr Asp Gly Asp Tyr Trp Gly Gln Gly Thr Ser
Val Thr Val Ser 100 105 110 Ser <210> SEQ ID NO 35
<211> LENGTH: 111 <212> TYPE: PRT <213> ORGANISM:
Mus musculus <400> SEQUENCE: 35 Asp Ile Leu Leu Thr Gln Thr
Pro Ala Ser Leu Ala Val Ser Leu Gly 1 5 10 15 Gln Arg Ala Thr Ile
Ser Cys Lys Ala Ser Gln Ser Val Asp Tyr Asp 20 25 30 Gly Asp Ser
Tyr Leu Asn Trp Tyr Gln Gln Ile Pro Gly Gln Pro Pro 35 40 45 Lys
Leu Leu Ile Tyr Asp Ala Ser Asn Leu Val Ser Gly Ile Pro Pro 50 55
60 Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Asn Ile His
65 70 75 80 Pro Val Glu Lys Val Asp Ala Ala Thr Tyr His Cys Gln Gln
Ser Thr 85 90 95 Glu Asp Pro Trp Thr Phe Gly Gly Gly Thr Lys Leu
Glu Ile Lys 100 105 110 <210> SEQ ID NO 36 <211>
LENGTH: 124 <212> TYPE: PRT <213> ORGANISM: Mus
musculus <400> SEQUENCE: 36 Gln Val Gln Leu Gln Gln Ser Gly
Ala Glu Leu Val Arg Pro Gly Ser 1 5 10 15 Ser Val Lys Ile Ser Cys
Lys Ala Ser Gly Tyr Ala Phe Ser Ser Tyr 20 25 30 Trp Met Asn Trp
Val Lys Gln Arg Pro Gly Gln Gly Leu Glu Trp Ile 35 40 45 Gly Gln
Ile Trp Pro Gly Asp Gly Asp Thr Asn Tyr Asn Gly Lys Phe 50 55 60
Lys Gly Lys Ala Thr Leu Thr Ala Asp Glu Ser Ser Ser Thr Ala Tyr 65
70 75 80 Met Gln Leu Ser Ser Leu Ala Ser Glu Asp Ser Ala Val Tyr
Phe Cys 85 90 95 Ala Arg Arg Glu Thr Thr Thr Val Gly Arg Tyr Tyr
Tyr Ala Met Asp 100 105 110 Tyr Trp Gly Gln Gly Thr Ser Val Thr Val
Ser Ser 115 120 <210> SEQ ID NO 37 <211> LENGTH: 107
<212> TYPE: PRT <213> ORGANISM: Mus musculus
<400> SEQUENCE: 37 Ala Ile Gln Leu Thr Gln Ser Pro Ser Ser
Leu Ser Ala Ser Val Gly 1 5 10 15 Asp Arg Val Thr Ile Thr Cys Arg
Ala Ser Gln Gly Ile Ser Ser Ala 20 25 30 Leu Ala Trp Tyr Gln Gln
Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 35 40 45 Tyr Asp Ala Ser
Ser Leu Glu Ser Gly Val Pro Ser Arg Phe Ser Gly 50 55 60 Ser Gly
Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 65 70 75 80
Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Phe Asn Ser Tyr Pro Tyr 85
90 95 Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys 100 105
<210> SEQ ID NO 38 <211> LENGTH: 120 <212> TYPE:
PRT <213> ORGANISM: Mus musculus <400> SEQUENCE: 38 Glu
Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Glu 1 5 10
15 Ser Leu Lys Ile Ser Cys Lys Gly Ser Gly Tyr Ser Phe Ser Ser Ser
20 25 30 Trp Ile Gly Trp Val Arg Gln Met Pro Gly Lys Gly Leu Glu
Trp Met 35 40 45 Gly Ile Ile Tyr Pro Asp Asp Ser Asp Thr Arg Tyr
Ser Pro Ser Phe 50 55 60 Gln Gly Gln Val Thr Ile Ser Ala Asp Lys
Ser Ile Arg Thr Ala Tyr
65 70 75 80 Leu Gln Trp Ser Ser Leu Lys Ala Ser Asp Thr Ala Met Tyr
Tyr Cys 85 90 95 Ala Arg His Val Thr Met Trp Gly Val Ile Ile Asp
Phe Trp Gly Gln 100 105 110 Gly Thr Leu Val Thr Val Ser Ser 115 120
<210> SEQ ID NO 39 <211> LENGTH: 112 <212> TYPE:
PRT <213> ORGANISM: Mus musculus <400> SEQUENCE: 39 Asp
Ile Val Met Thr Gln Ala Ala Pro Ser Ile Pro Val Thr Pro Gly 1 5 10
15 Glu Ser Val Ser Ile Ser Cys Arg Ser Ser Lys Ser Leu Leu Asn Ser
20 25 30 Asn Gly Asn Thr Tyr Leu Tyr Trp Phe Leu Gln Arg Pro Gly
Gln Ser 35 40 45 Pro Gln Leu Leu Ile Tyr Arg Met Ser Asn Leu Ala
Ser Gly Val Pro 50 55 60 Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr
Ala Phe Thr Leu Arg Ile 65 70 75 80 Ser Arg Val Glu Ala Glu Asp Val
Gly Val Tyr Tyr Cys Met Gln His 85 90 95 Leu Glu Tyr Pro Phe Thr
Phe Gly Ala Gly Thr Lys Leu Glu Leu Lys 100 105 110 <210> SEQ
ID NO 40 <211> LENGTH: 121 <212> TYPE: PRT <213>
ORGANISM: Mus musculus <400> SEQUENCE: 40 Glu Val Gln Leu Gln
Gln Ser Gly Pro Glu Leu Ile Lys Pro Gly Ala 1 5 10 15 Ser Val Lys
Met Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Ser Tyr 20 25 30 Val
Met His Trp Val Lys Gln Lys Pro Gly Gln Gly Leu Glu Trp Ile 35 40
45 Gly Tyr Ile Asn Pro Tyr Asn Asp Gly Thr Lys Tyr Asn Glu Lys Phe
50 55 60 Lys Gly Lys Ala Thr Leu Thr Ser Asp Lys Ser Ser Ser Thr
Ala Tyr 65 70 75 80 Met Glu Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala
Val Tyr Tyr Cys 85 90 95 Ala Arg Gly Thr Tyr Tyr Tyr Gly Ser Arg
Val Phe Asp Tyr Trp Gly 100 105 110 Gln Gly Thr Thr Leu Thr Val Ser
Ser 115 120
* * * * *