U.S. patent application number 15/745949 was filed with the patent office on 2018-07-12 for ultraviolet blocking agent and cosmetic product.
This patent application is currently assigned to SHODOSHIMA HEALTHYLAND CO., LTD.. The applicant listed for this patent is SHODOSHIMA HEALTHYLAND CO., LTD.. Invention is credited to Kana IWATA, Norihito KISHIMOTO, Toshihiro YAGYU.
Application Number | 20180193251 15/745949 |
Document ID | / |
Family ID | 58797163 |
Filed Date | 2018-07-12 |
United States Patent
Application |
20180193251 |
Kind Code |
A1 |
YAGYU; Toshihiro ; et
al. |
July 12, 2018 |
ULTRAVIOLET BLOCKING AGENT AND COSMETIC PRODUCT
Abstract
A new ultraviolet blocking agent and a cosmetic product having a
high ultraviolet blocking action. The inventors have found that the
extract obtained from branches of olive has a high effect of
absorbing ultraviolet, in particular UV-A and UV-B and has a high
ultraviolet absorbing action, and completed. The ultraviolet
blocking agent contains an extract of branches of olive. The
branches of olive may be bark of olive. The ultraviolet blocking
agent may be in the form of a cosmetic product.
Inventors: |
YAGYU; Toshihiro;
(Shozu-gun, JP) ; KISHIMOTO; Norihito; (Shozu-gun,
JP) ; IWATA; Kana; (Shozu-gun, JP) |
|
Applicant: |
Name |
City |
State |
Country |
Type |
SHODOSHIMA HEALTHYLAND CO., LTD. |
Shozu-gun, Kagawa |
|
JP |
|
|
Assignee: |
SHODOSHIMA HEALTHYLAND CO.,
LTD.
Shozu-gun, Kagawa
JP
|
Family ID: |
58797163 |
Appl. No.: |
15/745949 |
Filed: |
December 1, 2016 |
PCT Filed: |
December 1, 2016 |
PCT NO: |
PCT/JP2016/084502 |
371 Date: |
January 18, 2018 |
Current U.S.
Class: |
1/1 |
Current CPC
Class: |
A23L 33/105 20160801;
A61P 39/00 20180101; A61P 17/00 20180101; A61K 8/9789 20170801;
A23V 2002/00 20130101; A61P 17/16 20180101; A61K 36/63 20130101;
A23L 33/10 20160801; A61K 8/602 20130101; A61Q 17/04 20130101 |
International
Class: |
A61K 8/9789 20060101
A61K008/9789; A61K 36/63 20060101 A61K036/63; A61Q 17/04 20060101
A61Q017/04; A23L 33/105 20060101 A23L033/105 |
Foreign Application Data
Date |
Code |
Application Number |
Dec 1, 2015 |
JP |
2015-234470 |
Claims
1. An ultraviolet blocking agent comprising an extract of branches
of olive.
2. The ultraviolet blocking agent according to claim 1, wherein a
total polyphenol content per 100 g of the ultraviolet blocking
agent is not less than 100 mg.
3. The ultraviolet blocking agent according to claim 1 wherein the
branches of olive are bark of olive.
4. The ultraviolet blocking agent according to claim 1, wherein the
ultraviolet blocking agent is in a form of a cosmetic product.
5. A method of blocking ultraviolet radiation to a target, the
method comprising administering an extract of branches of
olive.
6. A method of blocking ultraviolet radiation to a target, the
method comprising administering an extract of branches of olive to
skin.
7. The ultraviolet blocking agent according to claim 2, wherein the
branches of olive are bark of olive.
8. The ultraviolet blocking agent according to claim 2, wherein the
ultraviolet blocking agent is in a form of a cosmetic product.
9. The ultraviolet blocking agent according to claim 3, wherein the
ultraviolet blocking agent is in a form of a cosmetic product.
10. The ultraviolet blocking agent according to claim 7, wherein
the ultraviolet blocking agent is in a form of a cosmetic product.
Description
TECHNICAL FIELD
[0001] The present invention relates to an ultraviolet blocking
agent and a cosmetic product.
BACKGROUND ART
[0002] Ultraviolet is electromagnetic waves of invisible light rays
with wavelengths of 10 nm to 400 nm and has various chemical
effects. In particular, near-ultraviolet with wavelengths of about
380 nm to 200 nm is classified into UV-A (320 nm to 400 nm), UV-B
(290 nm to 320 nm), and UV-C (200 nm to 290 nm) in terms of the
effects on human health and environment. UV-A and UV-B included in
sunlight pass through the ozone layer to reach the earth's surface.
UV-A is known to reach the dermis layer of skin to denature
protein, causing the skin to lose its elasticity and accelerating
aging. UV-B is known to act on the epidermis layer of skin to
generate melanin in pigment cells and cause sunburn. Ultraviolet
may cause diseases such as skin cancer.
[0003] In order to suppress these effects by ultraviolet, a variety
of substances having the ultraviolet blocking action are disclosed.
For example, Patent Document 1 describes a fiber or a fiber
structure having tea polyphenols fixed thereon to absorb and
suppress ultraviolet in the invisible wavelength range included in
sunlight.
[0004] Olive is a plant in the family of Oleaceae, originated from
the Mediterranean Coast and traditionally cultivated for eating and
for obtaining oil. The olive fruit ripe around December contains
about 15% to 30% of oil, and olive oil is obtained by pressing the
olive fruit in this season. The thus obtained olive fruit and olive
oil are known to have various excellent effects such as alleviating
arteriosclerosis, gastric ulcer, and obstipation, strengthening
bones, preventing aging, and skin-beautifying actions.
[0005] Olive leaves are known to have a vitamin A content much
higher than the olive fruit and be rich in vitamin E as an
antioxidant as well as chlorophyll and others having an
anti-inflammatory action and deodorant and antibacterial
actions.
[0006] The olive fruit and leaves contain polyphenols, and, for
example, their health enhancing actions have also drawn attention.
Oleuropein, which is a kind of polyphenols contained in olive
leaves, particularly has a very high antioxidant potency, and the
effect of preventing and ameliorating various diseases have been
noted.
[0007] As a method of extracting an olive leaf extract, for
example, Patent Document 2 discloses a method of producing an olive
leaf extract including oleuropein by drying and grinding olive
leaves, followed by extraction using water, water containing citric
acid, or water containing peptide as an extraction solvent.
PRIOR ART DOCUMENTS
[0008] Patent Document
[0009] Patent Document 1: Japanese Patent Application Publication
No. 2001-336064 (JP 2001-336064 A)
[0010] Patent Document 2: Japanese Patent Application Publication
No. 2011-125301 (JP 2011-125301 A)
SUMMARY OF THE INVENTION
Problem to be Solved by the Invention
[0011] An object of the present invention is to provide a new
ultraviolet blocking agent and a cosmetic product having a high
ultraviolet blocking action.
Means for Solving the Problem
[0012] The inventors of the present invention have found that the
extract obtained from branches of olive has a high effect of
absorbing ultraviolet, in particular, UV-A and UV-B and has a high
ultraviolet absorbing action. The inventors of the present
invention have completed the invention based on this finding.
[0013] The present invention provides an ultraviolet blocking agent
comprising an extract of branches of olive.
[0014] The present invention provides an ultraviolet blocking
agent, in which a total polyphenol content per 100 g of the
ultraviolet blocking agent is not less than 100 mg.
[0015] The present invention provides an ultraviolet blocking
agent, in which the branches of olive are bark of olive.
[0016] The present invention provides an ultraviolet blocking agent
in a form of a cosmetic product.
[0017] The present invention further provides a method of blocking
ultraviolet radiation to a target, comprising applying an extract
of branches of olive to skin.
Effects of the Invention
[0018] The present invention can provide an ultraviolet blocking
agent and a cosmetic product having a high ultraviolet blocking
action.
BRIEF DESCRIPTION OF THE DRAWINGS
[0019] FIG. 1 is a graph illustrating the total polyphenol content
(mg/100 g) and the oleuropein content (mg/100 g) of olive branch
extracts extracted using a variety of extraction solvents.
[0020] FIG. 2 is a graph illustrating the total polyphenol content
(mg/100 g) and the oleuropein content (mg/100 g) of olive branch
extracts extracted from a variety of cultivars of olive
branches.
[0021] FIG. 3 is a graph illustrating the total polyphenol content
(mg/100 g) and the oleuropein content (mg/100 g) of olive branch
extracts extracted at a variety of extraction temperatures.
[0022] FIG. 4 is a graph illustrating the total polyphenol content
(mg/100 g) and the oleuropein content (mg/100 g) of olive branch
extracts extracted with a variety of extraction times.
[0023] FIG. 5 is a graph illustrating the total polyphenol content
(mg/100 g) and the oleuropein content (mg/100 g) of olive branch
extracts extracted with a variety of amounts of branch added.
MODES FOR CARRYING OUT THE INVENTION
[0024] The present invention provides an ultraviolet blocking agent
containing an extract of branches of olive (which hereinafter may
be referred to as "olive branch extract").
[0025] The olive branch extract is an extract derived from branches
of olive. In the present description, "branches of olive" or "olive
branches" includes branches, stems, and bark of olive trees. That
is, in the present description, "olive branch extract" includes
olive branch extract, olive stem extract, and olive bark extract.
The extract of branches of olive contains many polyphenols such as
oleuropein and hydroxytyrosol.
[0026] Examples of the cultivars of branches of olive that may be
used in the present invention include Lucca, Mission, Nevadillo
Blanco, Manzanillo, Amellenque, Arbequina, Ascolana Terena,
Ascolano, Azapa, Barnea, Barouni, Biancolilla, Bidh El Hamman,
Blanqueta, Caillet Blane, Carolea, Cayonne, Chemilali, Chitoni,
Cipressino, Coratina, Cornicabra, Correggiola, Cucco, Gigante di
Cerignola, Frantoio, Glappolo, Gordal, Hardy's Mammoth, Hojiblanca,
Itrana, Jumbo Kalamata, Kalamata, Koroneiki, Leccino, Leccio del
Corno, Liani, Lucques, Manzanilla, Maurino, Michellenque, Moraiolo,
Nabali Mohassan, Nab Tamri, Negral, Nocellara del Belice, Obliza,
Oblonga, Paragon, Pendolino, Picual, Redding picholine, Redounan,
Saurin large leaf, Saurin medium leaf, Saurin small leaf,
Sevillano, Sorani, South Australian Verdale, St. Catherin,
Taggiasca, Tanche, Tiny Oil Kalamata, Tsunati, Verdale, Wagga
Verdale, Zarza, Oliviere, and FS17. In the present invention, the
cultivars of branches of olive may be used singly or in combination
of two or more.
[0027] The total polyphenol content per 100 g of the ultraviolet
blocking agent according to the present invention is, for example,
not less than 100 mg, preferably not less than 200 mg, more
preferably not less than 300 mg. The oleuropein content per 100 g
of the ultraviolet blocking agent according to the present
invention is not less than 30 mg, preferably not less than 50 mg,
more preferably not less than 100 mg.
[0028] As illustrated in Examples described later, the olive branch
extract has the effect of absorbing, in particular, UV-A and UV-B
of ultraviolet. Thus, the ultraviolet blocking agent according to
the present invention can effectively block UV-A and UV-B and
therefore can prevent the effects by ultraviolet, such as aging of
skin, sunburn, and skin cancer. The ultraviolet blocking agent
according to the present invention can be used for pharmaceutical
drugs, medicines, quasi-drugs, cosmetics, foods, and others having
an ultraviolet blocking action and, for example, can be used for
cosmetic products and skin drugs for external use having sunscreen
effects.
[0029] The ultraviolet blocking agent according to the present
invention can be in the form of a cosmetic product. The ultraviolet
blocking agent according to the present invention can be, for
example, basic skin care products such as skin toner, skin milk,
cream, serum, lotion, essence, hand cream, lip balm, and facial
mask; makeup cosmetic products such as lipstick, lip gloss,
foundation, makeup base, liquid foundation, makeup pressed powder,
blusher, face powder, eye shadow, mascara, eye liner, and eyebrow
makeup; cleansing cosmetic products such as facial wash, body
shampoo, shampoo, and soap; cosmetic products for hair or scalp
such as hair tonic, hair cream, rinse, conditioner, and
hairdressing; and bath products.
[0030] The ultraviolet blocking agent according to the present
invention can be in any of liquid, solid, emulsion, cream, gel, and
paste forms.
[0031] The ultraviolet blocking agent according to the present
invention may contain the olive branch extract alone or may further
contain another ingredient. Examples of another ingredient include
oily ingredients, surfactants (synthetic and natural),
moisturizers, thickeners, preservatives, germicides, powder
ingredients, ultraviolet absorbers, antioxidants, pigments, and
fragrance. Other physiologically active ingredients may be further
contained as long as the effects of the ultraviolet blocking agent
according to the present invention can be retained.
[0032] Here, the oily ingredients that can be used include, for
example, plant-derived oils and fats such as olive oil, jojoba oil,
castor oil, soybean oil, rice oil, rice germ oil, coconut oil, palm
oil, cacao oil, meadowfoam oil, shea butter, tea tree oil, avocado
oil, macadamia nut oil, and plant-derived squalane; animal-derived
oils and fats such as mink oil and turtle oil; waxes such as
beeswax, carnauba wax, rice wax, and lanolin; hydrocarbons such as
liquid paraffin, Vaseline, paraffin wax, and squalane; fatty acids
such as myristic acid, palmitic acid, stearic acid, oleic acid,
isostearic acid, and cis-11-eicosenoic acid; higher alcohols such
as lauryl alcohol, cetanol, and stearyl alcohol; synthetic esters
and synthetic triglycerides such as isopropyl myristate, isopropyl
palmitate, butyl oleate, 2-ethylhexyl glyceride, and higher fatty
acid octyldodecyl (such as octyldodecyl stearate).
[0033] The surfactants that can be used include, for example,
nonionic surfactants such as polyoxyethylene alkyl ethers,
polyoxyethylene fatty esters, polyoxyethylene sorbitan fatty
esters, glycerol fatty esters, polyglycerol fatty esters,
polyoxyethylene glycerol fatty esters, polyoxyethylene hydrogenated
castor oils, and polyoxyethylene sorbitol fatty esters; anionic
surfactants such as fatty acid salts, alkyl sulfates, alkyl benzene
sulfonates, polyoxyethylene alkyl ether sulfates, polyoxyethylene
fatty amine sulfates, polyoxyethylene alkyl phenyl ether sulfates,
polyoxyethylene alkyl ether phosphates, .alpha.-sulfonated fatty
acid alkyl ester salts, and polyoxyethylene alkyl phenyl ether
phosphates; cationic surfactants such as quaternary ammonium salts,
primary to tertiary fatty amine salts, trialkylbenzylammonium
salts, alkyl pyridinium salts, 2-alkyl-1-alkyl-1-hydroxy ethyl
imidazolinium salts, N,N-dialkylmorpholinium salts, and
polyethylene polyamine fatty acid amide salts; and amphoteric
surfactants such as N,N-dimethyl-N-alkyl-N-carboxymethyl
ammoniobetaines, N,N,N-trialkyl-N-alkylene ammoniocarboxybetaines,
and N-acylamidopropyl-N',N'-dimethyl-N'.beta.-hydroxypropyl
ammoniosulfobetaines.
[0034] Furthermore, as an emulsifier or an emulsifier assistant,
for example, stevia derivatives such as enzymatically modified
stevia, lecithin and derivatives thereof, lacto-fermented rice,
lacto-fermented germinated rice, lacto-fermented grain (for
example, wheat, beans, and cereals), and Rhamnaceae zizyphus
joazeiro extract may be blended.
[0035] The moisturizers that can be used include, for example,
glycerol, propylene glycol, dipropylene glycol, 1,3-butylene
glycol, polyethylene glycols, sorbitols, xylitol, and sodium
pyrrolidone carboxylate. Other examples include saccharides such as
trehalose, lacto-fermented rice, mucopolysaccharides (for example,
hyaluronic acid and derivatives thereof, chondroitin and
derivatives thereof, and heparin and derivatives thereof), elastin
and derivatives thereof, collagen and derivatives thereof,
NMF-related substances, lactic acid, urea, higher fatty acid
octyldodecyl, seaweed extracts, urn orchid extract, seafood-derived
collagen and derivatives thereof, and a variety of amino acids and
derivatives thereof.
[0036] The thickeners that can be used include, for example, brown
algae, green algae, or red algae-derived ingredients such as
alginic acid, agar, carrageenan, and fucoidan; polysaccharides such
as urn orchid extract, pectin, locust bean gum, and aloe
polysaccharides; gums such as xanthan gum, tragacanth gum, and guar
gum; cellulose derivatives such as carboxymethyl cellulose and
hydroxyethyl cellulose; synthetic polymers such as polyvinyl
alcohols, polyvinyl pyrrolidones, carboxyvinyl polymers, and
acrylate/methacrylate copolymers; hyaluronic acid and derivatives
thereof, and polyglutamic acids and derivatives thereof.
[0037] The preservatives and the germicides that can be used
include, for example, urea; p-oxybenzoates such as methyl
p-oxybenzoate, ethyl p-oxybenzoate, propyl p-oxybenzoate, and butyl
p-oxybenzoate; phenoxyethanol, dichlorophen, hexachlorophene,
chlorhexidine hydrochloride, benzalkonium chloride, salicylic acid,
ethanol, undecylenic acid, phenols, Germall (imidazolidinyl urea),
1,2-pentanediol, various essential oils, and birch tar.
[0038] The powder ingredients that can be used include, for
example, sericite, titanium oxide, talc, kaoline, bentonite, zinc
oxide, magnesium carbonate, magnesium oxide, zirconium oxide,
barium sulfate, silicic acid anhydride, mica, nylon powder,
polyethylene powder, silk powder, cellulose powder, grain (for
example, rice, wheat, corn, and proso millet) powder, and bean (for
example, soybean and Adzuki bean) powder.
[0039] The ultraviolet absorbers that can be used include, for
example, ethyl p-aminobenzoate, ethylhexyl p-dimethyl
aminobenzoate, amyl salicylate and derivatives thereof,
2-ethylhexyl p-methoxycinnamate, octyl cinnamate, oxybenzone,
2,4-dihydroxybenzophenone,
2-hydroxy-4-methoxybenzophenone-5-sulfonate,
4-tertiarybutyl-4-methoxybenzoylmethane,
2-(2-hydroxy-5-methylphenyl)benzotriazole, urocanic acid, ethyl
urocanate, and aloe extract.
[0040] The antioxidants that can be used include, for example,
butyl hydroxyanisole, butyl hydroxytoluene, propyl gallate, vitamin
E and derivatives thereof, urn orchid extract, and Asian rice
extract.
[0041] The physiologically active ingredients that can be used
include, for example, as whitening ingredients, t-cycloamino acid
derivatives, kojic acid and derivatives thereof, ascorbic acid and
derivatives thereof, hydroquinone derivatives, ellagic acid and
derivatives thereof, resorcinol derivatives, mulberry bark extract,
Saxifraga stolonifera extract, rice bran extract, rice bran extract
hydrolysate, lacto-fermented rice, lacto-fermented germinated rice,
lacto-fermented grain (wheat, beans, cereals), white mustard
hydrolysis extract, Callicarpa japonica extract, Pandanus
amaryllifolius Roxb. extract, Arcangelicia flava Merrilli extract,
chamomile extract (tradename; CHAMOMILLA ET), extract of seaweed
such as Laminaria, extract of seagrass such as Zosteraceae,
linoleic acid and derivatives or processed products thereof (for
example, liposomal linoleic acid), and 2,5-dihydroxybenzoic acid
derivatives. Other examples include, as skin aging-preventing and
skin-beautifying ingredients, animal or fish-derived collagen and
derivatives thereof, elastin and derivatives thereof, nicotinic
acid and derivatives thereof, glycyrrhizic acid and derivatives
thereof (for example, dipotassium salt), t-cycloamino acid
derivatives, vitamin A and derivatives thereof, vitamin E and
derivatives thereof, allantoin, .alpha.-hydroxy acids,
diisopropylamine dichloroacetate,
.gamma.-amino-.beta.-hydroxybutyric acid, crude drug extracts such
as Gentiana lutea extract, Glycyrrhiza extract, Job's tears
extract, chamomile extract, Kudzu root extract, Shiso extract,
carrot extract, and aloe extract, rice extract hydrolysate, rice
bran extract hydrolysate, rice ferment extract, chlorella extract,
brown algae extract, Laminaria angustata extract, Ulva pertusa
extract, extract of seagrass such as Zostera marina, mulberry bark
extract, and Rhamnaceae zizyphus joazeiro extract.
[0042] Examples of the kojic acid derivatives include kojic acid
esters such as kojic acid monobutylate, kojic acid monocaprate,
kojic acid monopalmitate and kojic acid dibutylate, kojic acid
ethers, and kojic acid saccharide derivatives such as kojic acid
glucoside. Examples of the ascorbic acid derivatives include
ascorbic acid ester salts such as sodium L-ascorbic
acid-2-phosphate, magnesium L-ascorbic acid-2-phosphate, sodium
L-ascorbic acid-2-sulfate, and magnesium L-ascorbic acid-2-sulfate,
ascorbic acid saccharide derivatives such as L-ascorbic
acid-2-glucoside (2-0-.alpha.-D-glucopyranosyl-L-ascorbic acid) and
L-ascorbic acid-5-glucoside
(5-0-.alpha.-D-glucopyranosyl-L-ascorbic acid), the 6-position
acylated products (acyl group is, for example, hexanoyl group,
octanoyl group, or decanoyl group) of these ascorbic acid
saccharide derivatives, L-ascorbic acid tetra fatty acid esters
such as L-ascorbyl tetraisopalmitate and L-ascorbyl tetralaurate,
3-0-ethyl ascorbic acid, and sodium
L-ascorbyl-2-phosphate-6-0-palmitate. Examples of the hydroquinone
derivatives include arbutin (hydroquinone-.beta.-D-glucopyranoside)
and .alpha.-arbutin (hydroquinone-.alpha.-D-glucopyranoside).
Examples of the resorcinol derivatives include 4-n-butylresorcinol
and 4-isoamylresorcinol. Examples of the 2,5-dihydroxybenzoic acid
derivatives include 2,5-diacethoxybenzoic acid,
2-acethoxy-5-hydroxybenzoic acid, and
2-hydroxy-5-propionyloxybenzoic acid. Examples of the nicotinic
acid derivatives include nicotinamide and benzyl nicotinate.
Examples of the vitamin E derivatives include vitamin E nicotinate
and vitamin E linoleate. Examples of the .alpha.-hydroxy acid
include lactic acid, malic acid, succinic acid, citric acid, and
.alpha.-hydroxyoctanoic acid.
[0043] (Extraction Method of Olive Branch Extract)
[0044] The olive branch extract can be an extract derived from
branches of olive by any method. The olive branch extract may be
extracted, for example, from branches of olive using an extraction
solvent. The extraction solvent may be, for example, water and/or
alcohol.
[0045] An embodiment of the method of extracting an olive branch
extract for use in the ultraviolet blocking agent according to the
present invention will be described below.
[0046] The extract of branches of olive can be produced by, for
example, a production method comprising a first step of grinding
dried olive branches and a second step of extracting an olive
branch extract from the ground olive branches using an extraction
solvent.
[0047] The first step is a step of grinding dried olive branches.
In the present description, "grinding" refers to physically
crushing an object to reduce the size of the object, for example,
physically crushing an object into fine particles, small pieces,
powder, or the like. The method of drying olive branches and the
method of grinding olive branches are not limited to particular
methods, and any method can be used.
[0048] The picking seasons for olive branches are preferably, for
example, but not limited to, the times around December when the
olive fruit contains a high oil content in regions such as Japan in
the Northern Hemisphere, or preferably the times after the olive
fruit is fully ripe and harvested.
[0049] The second step is a step of extracting an olive branch
extract from the ground olive branches using an extraction solvent.
The extraction solvent includes water and/or alcohol. The alcohol
includes, for example, methanol, ethanol, isopropyl alcohol,
butanol, glycerol, ethylene glycol, propylene glycol, 1,3-butylene
glycol, and polyethylene glycols. The extraction solvent may be
water, may be 100% of alcohol, or may be alcohol diluted with
water. The extraction solvent may contain 10% to 50% of alcohol.
The extraction solvent may contain one alcohol alone or may include
two or more alcohols. The extraction solvent may contain
ingredients other than water and alcohol and may be, for example,
alcohol beverages such as sake and shochu (distilled spirit). The
extraction solvent may be, for example, water, ethanol or
1,3-butylene glycol, or a combination thereof.
[0050] In the second step, the extraction temperature may be
70.degree. C. or higher. Extraction at 70.degree. C. or higher can
yield an olive branch extract having a high polyphenol content and
a high ultraviolet absorption capacity, as illustrated in Examples
later. The extraction temperature may be preferably 80.degree. C.
or higher, more preferably 90.degree. C. or higher. The upper limit
of the extraction temperature may be, for example, but not limited
to, 100.degree. C. or lower.
[0051] The amount of olive branches added with respect to the
extraction solvent may be, for example, but not limited to, not
less than 20% by weight, preferably not less than 30% by weight.
The upper limit of the amount of branches added may be, for
example, but not limited to, not more than 50% by weight. This
amount of branches added can yield an olive branch extract having a
high polyphenol content and a high ultraviolet absorption capacity,
as illustrated in Examples later.
[0052] The extract of branches of olive in the ultraviolet blocking
agent according to the present invention can be produced by the
production method as described above to have an ultraviolet
absorption capacity higher than conventional ones. The ultraviolet
blocking agent according to the present invention therefore can be
used in a variety of pharmaceutical drugs, medicines, foods, and
cosmetics utilizing this ultraviolet absorption capacity.
[0053] The present invention also provides a method of blocking
ultraviolet radiation to a target, including administering the
ultraviolet blocking agent to the target's skin. The present
invention also provides a method of suppressing adverse effects on
a target caused by ultraviolet, including applying the ultraviolet
blocking agent to the target's skin. The adverse effects on a
target caused by ultraviolet refer to any symptom caused by
ultraviolet, in particular UV-A and UV-B, such as sunburn,
freckles, inflammation of skin, and skin photoaging.
[0054] The target to which the method according to the present
invention is applied includes mammals such as humans, mice, rats,
rabbits, cats, dogs, cows, horses, and monkeys.
[0055] In the method according to the present invention, the
ultraviolet blocking agent can be any ultraviolet blocking agent as
described above. In the method according to the present invention,
the ultraviolet blocking agent may be provided additionally with
any other ingredients. In the method according to the present
invention, any other ingredients include, for example, the
above-noted pharmaceutically acceptable bases, carriers,
excipients, binders, disintegrants, lubricants, and colorants.
[0056] In the method according to the present invention, the
ultraviolet blocking agent may be applied to a target through
dermal administration such as liniments and patches. In the method
according to the present invention, the ultraviolet blocking agent
may be administered such that a daily intake is 0.1 mg to 2,000 mg
per adult. In the method according to the present invention, the
ultraviolet blocking agent may be applied, but not limited to, once
to seven days a week. For example, in the method according to the
present invention, the ultraviolet blocking agent may be applied
daily, or five or six times a week. For example, when there is a
possibility of ultraviolet radiation outdoors, the ultraviolet
blocking agent in the form of dermal administration can be applied
to the target's skin as appropriate.
EXAMPLES
[0057] (Measurement Method)
[0058] In measuring the total polyphenol content, the
Folin-Ciocalteu method was used. This method uses the Folin's
phenol reagent, which is reduced by phenolic hydroxy group to
change color. In measuring the oleuropein content, HPLC analysis
was conducted. This is called high-performance liquid
chromatography and is a process for separating a certain substance
in a system including a stationary phase and a mobile phase.
[0059] In measuring the antioxidant potency, ORAC (Oxygen Radical
Absorbance Capacity) was determined (reference: Wu, X. et al., J.
Agric. Food Chem., m 2004, 52, 4026-4037. The activity exhibited by
1 .mu.mol of Trolox was used as a unit).
[0060] In measuring the ultraviolet absorption capacity, a
spectrophotometer was used to measure the absorption spectra. The
areas of the absorption spectra of ultraviolet regions (UV-A waves:
320 nm-400 nm, UV-B waves: 290 nm-320 nm) were measured and
determined as ultraviolet absorption capacity.
[0061] [Olive Branch Extract]
[0062] (Extraction Solvent)
[0063] Olive branch extracts were extracted from dried and ground
olive branches using water, 10% of 1,3-butylene glycol (BG), 30% of
BG, 50% of BG, 80% of BG, 10% of ethanol (EtOH), 30% of EtOH, 50%
of EtOH, and 100% of EtOH as extraction solvents. The cultivar of
olive branches used was Mission. The olive branches were added in
the amount of 10% by weight with respect to the extraction solvent
and subjected to extraction at extraction temperatures of
50.degree. C. to 60.degree. C. for 3 hours. The total polyphenol
content (mg/100 g), the oleuropein content (mg/100 g), and the
ultraviolet absorption capacity of the resultant olive branch
extract were measured and listed in Table 1 and illustrated in FIG.
1.
[0064] The result indicates that an olive branch extract having a
high total polyphenol content, a high oleuropein content, and a
high ultraviolet absorption capacity can be obtained using any of
water, 1,3-butylene glycol, and ethanol as an extraction solvent.
When 1,3-butylene glycol or ethanol is used as an extraction
solvent, the total polyphenol content, the oleuropein content, and
the ultraviolet absorption capacity are higher when using 10% to
50% of 1,3-butylene glycol or ethanol.
TABLE-US-00001 TABLE 1 Total UV-A waves UV-B waves polyphenol (320
nm-400 (290 nm-320 Extraction content nm) absorption nm) absorption
solvent (mg/100 g) capacity capacity Water 50 434 350 10% of BG 66
410 368 30% of BG 75 438 397 50% of BG 68 378 375 80% of BG 52 213
245 10% of EtOH 70 372 344 30% of EtOH 99 625 558 50% of EtOH 86
554 531 100% of EtOH 27 121 162
[0065] (Cultivar of Olive Branch)
[0066] Olive branch extracts were extracted from dried and ground
olive branches using 30% of 1,3-butylene glycol as an extraction
solvent. The cultivars of olive branches used were Mission, Lucca,
Nevadillo Banco, and Manzanillo. The olive branches were added in
the amount of 10% by weight with respect to the extraction solvent
and subjected to extraction at extraction temperatures of
50.degree. C. to 60.degree. C. for 3 hours. The total polyphenol
content (mg/100 g) and the oleuropein content (mg/100 g) of the
resultant olive branch extract were measured and illustrated in
FIG. 2.
[0067] The result indicates that an olive branch extract with a
high total polyphenol content can be obtained for any of the
cultivars of Mission, Lucca, Nevadillo Blanco, and Manzanillo.
[0068] (Extraction Temperature)
[0069] Olive branch extracts were extracted from dried and ground
olive branches using 30% of 1,3-butylene glycol as an extraction
solvent. The cultivar of olive branches used was Mission. The olive
branches were added in the amount of 10% by weight with respect to
the extraction solvent and subjected to extraction for 3 hours with
the extraction temperature changed stepwise in the range of
30.degree. C. to 100.degree. C. The total polyphenol content
(mg/100 g), the total amount of polyphenol per heat quantity (cal)
(mg/heat quantity cal), the oleuropein content (mg/100 g), and the
ultraviolet absorption capacity of the resultant olive branch
extract were measured and listed in Table 2 and illustrated in FIG.
3.
[0070] The result indicates that when the extraction temperature is
70.degree. C. or higher, an olive branch extract having a high
total polyphenol content, a high oleuropein content, and a high
ultraviolet absorption capacity can be extracted. It is also
demonstrated that when the extraction temperature is 80.degree. C.
or higher, preferably 90.degree. C. or higher, an olive branch
extract having a higher total polyphenol content and a higher
ultraviolet absorption capacity can be extracted.
TABLE-US-00002 TABLE 2 Total Total amount UV-A waves UV-B waves
polyphenol of polyphenol (320 nm-400 (290 nm-320 Extraction content
(mg/heat nm) absorption nm) absorption temperature (mg/100 g)
quantity cal) capacity capacity 30.degree. C.-40.degree. C. 60
0.015 320 310 40.degree. C.-50.degree. C. 59 0.012 293 277
50.degree. C.-60.degree. C. 75 0.012 438 397 60.degree.
C.-70.degree. C. 93 0.013 575 523 70.degree. C.-80.degree. C. 110
0.014 655 594 80.degree. C.-90.degree. C. 190 0.021 969 891
90.degree. C.-100.degree. C. 296 0.030 2183 1720
[0071] (Extraction Time)
[0072] Olive branch extracts were extracted from dried and ground
olive branches using 30% of 1,3-butylene glycol as an extraction
solvent. The cultivar of olive branches used was Mission. The olive
branches were added in the amount of 10% by weight with respect to
the extraction solvent and subjected to extraction at extraction
temperatures of 50.degree. C. to 60.degree. C. The extraction times
were 0.5 hour, 1 hour, 2 hours, 3 hours, and 5 hours. The total
polyphenol content (mg/100 g), the total amount of polyphenol per
the extraction time (mg/time), the oleuropein content (mg/100 g),
and the ultraviolet absorption capacity of the resultant olive
branch extract were measured and listed in Table 3 and illustrated
in FIG. 4.
[0073] The result indicates that the longer the extraction time is,
the higher the total polyphenol content and the ultraviolet
absorption capacity are, but the lower the total amount of
polyphenol per the extraction time is.
TABLE-US-00003 TABLE 3 Total UV-A waves UV-B waves polyphenol Total
amount (320 nm-400 (290 nm-320 Extraction content of polyphenol nm)
absorption nm) absorption time (mg/100 g) (mg/time) capacity
capacity 0.5 hour .sup. 40 238 141 160 1 hour.sup. 43 129 208 208 2
hours 65 98 361 332 3 hours 75 75 438 397 5 hours 92 55 498 461
[0074] (Amount of Branches Added)
[0075] Olive branch extracts were extracted from dried and ground
olive branches using 30% of 1,3-butylene glycol as an extraction
solvent. The cultivar of olive branches used was Mission. The olive
branches were added in the amount of 5%, 10%, 20%, 30%, 40%, or 44%
by weight with respect to the extraction solvent and subjected to
extraction at extraction temperatures of 50.degree. C. to
60.degree. C. for 3 hours. The total polyphenol content (mg/100 g),
the total amount of polyphenol per the amount of branches added (g)
(mg/added branches g), the oleuropein content (mg/100 g), and the
ultraviolet absorption capacity of the resultant olive branch
extract were measured and listed in Table 4 and illustrated in FIG.
5.
[0076] The result indicates that when the amount of branches added
is not less than 30% by weight, an olive branch extract having a
high total polyphenol content, a high oleuropein content, and a
high ultraviolet absorption capacity can be extracted.
TABLE-US-00004 TABLE 4 Total Total amount UV-A waves UV-B waves
polyphenol of polyphenol (320 nm-400 (290 nm-320 Amount of content
(mg/added nm) absorption nm) absorption branches added (mg/100 g)
branches g) capacity capacity 5% 33 6.6 119 142 10% 75 7.5 438 397
20% 154 7.7 965 802 30% 255 8.5 1692 1381 40% 330 8.3 2506 2041 44%
334 7.6 2162 1836
[0077] (Comparison of Extraction Conditions)
[0078] Olive branch extracts were extracted from dried and ground
olive branches using 30% of 1,3-butylene glycol as an extraction
solvent under two different extraction conditions. The cultivar of
olive branches used was Mission. In Extraction Condition 1
(Comparative Example), the olive branches were added in the amount
of 10% by weight with respect to the extraction solvent and
subjected to extraction at 50.degree. C. to 60.degree. C. for 3
hours. In Extraction Condition 2 (Example), the olive branches were
added in the amount of 30% by weight with respect to the extraction
solvent and subjected to extraction at 80.degree. C. to 90.degree.
C. for 2 hours. The total polyphenol content (mg/100 g), the
oleuropein content (mg/100 g), the ultraviolet absorption capacity,
and the antioxidant potency of the resultant olive branch extract
were measured and listed in Table 5.
[0079] The result indicates that an olive branch extract having a
significantly high total polyphenol content, a significantly high
oleuropein content, a significantly high ultraviolet absorption
capacity, and a significantly high antioxidant potency can be
obtained with Extraction Condition 2, compared with Extraction
Condition 1.
TABLE-US-00005 TABLE 5 Total UV-A waves UV-B waves antioxidant
polyphenol Oleuropein (320 nm-400 (290 nm-320 potency Condition
content content nm) absorption nm) absorption ORAC comparison
(mg/100 g) (mg/100 g) capacity capacity (.mu.mol TE/g) Extraction
75 18.6 438 397 14 Condition 1 Extraction 759 122.9 3249 2535 93
Condition 2
[0080] [Olive Bark Extract]
[0081] (Extraction Solvent)
[0082] Olive bark extracts were extracted from dried and ground
olive bark using water, 30% of 1,3-butylene glycol (BG), and 50% of
ethanol (EtOH) as extraction solvents. The cultivar of olive bark
used was Mission. The olive bark was added in the amount of 10% by
weight with respect to the extraction solvent and subjected to
extraction at extraction temperatures of 50.degree. C. to
60.degree. C. for 5 hours. The total polyphenol content (mg/100 g)
of the resultant olive bark extract was measured and listed in
Table 6.
[0083] The result indicates that an olive bark extract having a
high total polyphenol content can be extracted using any of water,
1,3-butylene glycol, and ethanol as an extraction solvent.
TABLE-US-00006 TABLE 6 Extraction Total polyphenol content solvent
(mg/100 g) Water 83 30% of BG 143 50% of EtOH 162
[0084] (Amount of Bark Added)
[0085] Olive bark extracts were extracted from dried and ground
olive bark using 30% of 1,3-butylene glycol (BG) as an extraction
solvent. The cultivar of olive bark used was Mission. The olive
bark was added in the amount of 5%, 10%, 20%, or 30% by weight with
respect to the extraction solvent and subjected to extraction at
extraction temperatures of 50.degree. C. to 60.degree. C. for 5
hours. The total polyphenol content (mg/100 g), the total amount of
polyphenol per the amount of bark added (g) (mg/added bark g), and
the ultraviolet absorption capacity of the resultant olive bark
extract were measured and listed in Table 7.
[0086] The result indicates that when the amount of bark added is
not less than 20% by weight, an olive bark extract having a high
total polyphenol content and a high ultraviolet absorption capacity
can be extracted.
TABLE-US-00007 TABLE 7 Total Total amount UV- A waves UV-B waves
polyphenol of polyphenol (320 nm-400 (290 nm-320 Amount of content
(mg/added nm) absorption nm) absorption bark added (mg/100 g) bark
g) capacity capacity 5% 86 17.3 712 510 10% 143 14.3 1113 814 20%
272 13.6 2419 1719 30% 284 9.5 2531 1876
[0087] (Extraction Temperature)
[0088] Olive bark extracts were extracted from dried and ground
olive bark using 30% of 1,3-butylene glycol as an extraction
solvent. The cultivar of olive bark used was Mission. The olive
bark was added in the amount of 10% by weight with respect to the
extraction solvent and subjected to extraction for 5 hours with
extraction temperature changed stepwise in the range of 40.degree.
C. to 90.degree. C. The total polyphenol content (mg/100 g), the
total amount of polyphenol per heat quantity (cal) (mg/heat
quantity cal), and the ultraviolet absorption capacity of the
resultant olive bark extract were measured and listed in Table
8.
[0089] The result indicates that when the extraction temperature is
70.degree. C. or higher, an olive bark extract having high total
polyphenol content and ultraviolet absorption capacity can be
extracted.
TABLE-US-00008 TABLE 8 Total Total amount UV-A waves UV-B waves
polyphenol of polyphenol (320 nm-400 (290 nm-320 Extraction content
(mg/heat nm) absorption nm) absorption temperature (mg/100 g)
quantity cal) capacity capacity 40.degree. C.-50.degree. C. 106
0.021 896 663 50.degree. C.-60.degree. C. 143 0.024 1113 814
60.degree. C.-70.degree. C. 178 0.025 1387 1062 70.degree.
C.-80.degree. C. 315 0.039 1733 1243 80.degree. C.-90.degree. C.
275 0.031 1750 1275
[0090] (Extraction Time)
[0091] Olive bark extracts were extracted from dried and ground
olive bark using 30% of 1,3-butylene glycol as an extraction
solvent. The cultivar of olive bark used was Mission. The olive
bark was added in the amount of 10% by weight with respect to the
extraction solvent and subjected to extraction at extraction
temperatures of 50.degree. C. to 60.degree. C. The extraction times
were 0.5 hour, 1 hour, 2 hours, 3 hours, and 5 hours. The total
polyphenol content (mg/100 g), the total amount of polyphenol per
the extraction time (mg/time), and the ultraviolet absorption
capacity of the resultant olive bark extract were measured and
listed in Table 9.
[0092] The result indicates that the longer the extraction time is,
the higher the total polyphenol content and the ultraviolet
absorption capacity are, but the lower the total amount of
polyphenol per the extraction time is.
TABLE-US-00009 TABLE 9 Total UV-A waves UV-B waves polyphenol Total
amount (320 nm-400 (290 nm-320 Extraction content of polyphenol nm)
absorption nm) absorption time (mg/100 g) (mg/time) capacity
capacity 0.5 hour .sup. 66 397 830 600 1 hour.sup. 84 252 993 714 2
hours 120 180 1142 818 3 hours 134 134 1044 763 5 hours 143 86 1113
814
[0093] (Comparison of Extraction Solvents Under Optimum
Conditions)
[0094] Olive bark extracts were extracted from olive bark using a
variety of extraction solvents under the optimum conditions derived
from the results above. Water, 30% of 1,3-butylene glycol, and 50%
of ethanol were used as extraction solvents. The extraction
temperatures were 70.degree. C. to 80.degree. C., the extraction
time was 5 hours, and the amount of bark added was 20% by weight
with respect to the extraction solvent. The total polyphenol
content (mg/100 g) and the ultraviolet absorption capacity of the
resultant olive bark extract were measured and listed in Table
10.
[0095] The result indicates that when 30% of 1,3-butylene glycol is
used as an extraction solvent, the total polyphenol content and the
ultraviolet absorption capacity are highest.
TABLE-US-00010 TABLE 10 Total UV-A waves UV-B waves polyphenol (320
nm-400 (290 nm-320 Extraction content nm) absorption nm) absorption
condition (mg/100 g) capacity capacity Water 151 2436 1851 30% of
BG 479 3766 2652 50% of EtOH 336 2672 1855
[0096] (Comparison of Extraction Conditions)
[0097] Olive bark extracts were extracted from dried and ground
olive bark using 30% of 1,3-butylene glycol as an extraction
solvent under two different extraction conditions. The cultivar of
olive bark used was Mission. In Extraction Condition 3 (Comparative
Example), the olive bark was added in the amount of 10% by weight
with respect to the extraction solvent and subjected to extraction
at 50.degree. C. to 60.degree. C. for 5 hours. In Extraction
Condition 4 (Example), the olive bark was added in the amount of
20% by weight with respect to the extraction solvent and subjected
to extraction at 70.degree. C. to 80.degree. C. for 5 hours. The
antioxidant potency of the resultant olive bark extract was
measured and listed in Table 11.
[0098] The result indicates that an olive bark extract having a
significantly high antioxidant potency can be obtained with
Extraction Condition 4, compared with Extraction Condition 3.
TABLE-US-00011 TABLE 11 Antioxidant potency Antioxidant potency
ORAC (.mu.mol TE/g) Extraction Condition 3 11 Extraction Condition
4 33
[0099] Although the embodiment according to the present invention
has been described above, it is needless to say that the present
invention is not limited thereto and is susceptible of various
modifications and changes without departing from the spirit of the
invention.
INDUSTRIAL APPLICABILITY
[0100] The ultraviolet blocking agent according to the present
invention can be suitably used in pharmaceutical drugs, medicines,
cosmetics, and foods having an ultraviolet blocking action.
* * * * *